LOCUS       DAOSYN010000001        19889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2236_length_19889_cov_4.479984, whole genome shotgun sequence.
ACCESSION   DAOSYN010000001 DAOSYN010000000
VERSION     DAOSYN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 19889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19889
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2236_length_19889_cov_4.479984"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PLJ11_00005"
     CDS             complement(<1..1145)
                     /locus_tag="PLJ11_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO03089.1"
                     /translation="MTPGTRKGREGRGHGRPGQRAKSFREAWRRMLAYMRPYLPAIIL
                     ASVLVVIGTVFTVIGPDRLRAMTDLIAEGMMTGTMDMEAIAGIGTFLVTIYLLAAVMT
                     YGQGYIVTTVVQKLSKRLRTDISRKINRLPLRYFDRTSYGDVLSRVTNDVDTLGQSMN
                     QSIGTLMSATALLIGALVMMLLTDGVMTAAAVLSALAGFALIAIIMSKSQKYFERQQL
                     HLGKLNGYVEETYTGHVIVKAYNGEAAAQREFDSMNDTLFDSAFRSLFLSGMMMPIMG
                     FIGNLGYVAVCIVGAVRVLDGAITIGTIVAFMVYVRLFTQPLSQLGQAMTSLQSVAAA
                     AERVFEFLDEPELEGEDHKVLVLKDVKGDVECRNVSFGYTPEREVIH"
     gene            complement(1142..>2627)
                     /locus_tag="PLJ11_00010"
     CDS             complement(1142..>2627)
                     /locus_tag="PLJ11_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO03090.1"
                     /translation="LEFSRVQAFSAGEINRFSTASLITRATNDVTQVQMAVAIGLQVV
                     IKAPILAGWAILKIAGKSWQWTTATAVAIAVLIAVVSVLMVFVVPRFKKVQWLTDEVN
                     RYMREGLKGIRVIRAFNAEEYQERKFERANEDLTANNLFAGRAMALMMPVMSAVMSLL
                     SLSIYWIGAIIIEATVGIGAQIARFSEMVTFLAYAMQVVMGFMMLVIVFIIMPRAMVA
                     AKRIEEVLDTEPSIKDGSVAAPEGPAAGEIVFDRVGFKYPGAVDYVLKDVSFTARKGE
                     TIAFIGSTGSGKSTIVNLILRLYDVTEGSIRVDGVDVRDYTQRALRRKIGYVPQRPVL
                     FSGTVASNVAYGDTAAERTLEDVKRAVAIAQAADFVEAMEGGYEASISQGGINISGGQ
                     KQRLSIARAICRRPEIYIFDDSFSALDYRTDRLLRSALKKETAGVTSIIVAQRVGTIM
                     DADRIVVLDSGMVVGIGRHSELLGTCPVYREIVNSQLSEEDLRR"
     assembly_gap    2628..2637
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2638..2894)
                     /locus_tag="PLJ11_00015"
     CDS             complement(<2638..2894)
                     /locus_tag="PLJ11_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO03091.1"
                     /translation="MILRYLKAREWALVGACAALITLQVWLDLEIPDYMYAITTLIQT
                     GGSVAQVMDQGWPMLACALGSLLTALAVGCMAAYIAASLAKR"
     gene            complement(2965..3471)
                     /locus_tag="PLJ11_00020"
     CDS             complement(2965..3471)
                     /locus_tag="PLJ11_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOO03092.1"
                     /translation="MVGKPHMYKGNRISPSQLIMMIELAERPMYGYELIKVLREEYDG
                     VWVPKTGAVYPALRRLQEHGLLTVRNIEGKDHYSLSPEGQAWLDETLAGMTSQVLFMA
                     RTTEIIGRAYLRRKGKVEEFIPPEERPPEVLLEELREVRKAMLANLAMLDGQIKELEG
                     ELEREGES"
     gene            3586..4359
                     /locus_tag="PLJ11_00025"
     CDS             3586..4359
                     /locus_tag="PLJ11_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03093.1"
                     /translation="MDLTFEEIPEDLWEDWVWLVSPAGLMWAVEEAAQPLLSTPYLLT
                     PIYTVNMPKMVLFHMAWSGDFDIDGDGKVRTEEPYPPVRMDADIALRGLLFLLRNYPL
                     VLRWKLEPDRLASLAPNVWDDVLEPPEALWHVPPELEGEPVDLENISIEFFNPFIQSL
                     RFRGVHRSLIGVVSPMRSIARATAALVPEAESDWREAMAMAIQELERRGLIEAVDAGL
                     YRFTERGARMTATEPVSDCLCCRCRIEEVVEYELGGDED"
     gene            4507..5430
                     /locus_tag="PLJ11_00030"
     CDS             4507..5430
                     /locus_tag="PLJ11_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03094.1"
                     /translation="MKLWDFGRVKDGKVRIGDKVRRRLGLEDGSQVYSTLFRYEQDGA
                     RGHEIVLSTFGPENYRELCNLTFYMIDAPGACAQAAKFLADRNVDILNSVSVSMTCGI
                     SMVWRMLVDLSYYGDSTQLMEDFASVRRASPAALSKVEGLEISHSHISDRYTKGMTGP
                     QPGRIKVKPLRKVARSPSLIKDESFTIPKDYLEQLEGVRDGTPVMMVGDVDSWVLSIS
                     FLPEDCHLAEFSFEIPDRPGSIYRTTDALATQGVNLLAVQSKVLVYGQRMTLDVVADL
                     KDAGELDKVVERTLMALGGEYRLRCKGPVTV"
     gene            5441..6199
                     /locus_tag="PLJ11_00035"
     CDS             5441..6199
                     /locus_tag="PLJ11_00035"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOO03095.1"
                     /translation="MLIGRKVLVKDEVDSTNDVAKALAVEGEPEGTVVTAARQTCGRG
                     RTGRRWESPRGGLYLSVILRPELPAPDLTLFTVLSCLPVAQAMEEVCRVVPSIKWPND
                     VRVEGKKVAGILGEVCYRGGEPRFMVLGIGIDLNTDPAALTAPEAGSLREICKRDLDP
                     ERFLNVLLFRLDDMYARFRKGERELDLYARYSESIGRDVEVRLGDQRVRGRGMRLDAD
                     GALVLRSEDGMIYRATSVHDVVPLDRGQVRFEGG"
     gene            6238..7785
                     /locus_tag="PLJ11_00040"
     CDS             6238..7785
                     /locus_tag="PLJ11_00040"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOO03096.1"
                     /translation="MGEDRIKELREMKKRSRAGGGAERVEAHHKQGKMTARERVDALL
                     DPGSFSELDTFVSHQAYHFGMDKQKVPGDGVVTGRGTIEGRQVFVYAQDFTVFAGSVG
                     RMHATKICKVIDLAVKTGCPVIALYDSGGARLQEGVDSLAGYGEVMLRHTLASGLIPQ
                     IAMVMGPCAGAGAYAPILSDFVFMVKDKSFMFMVGPDVIKAVQNETVTFEELGGASVH
                     ASRSGSAHFVAKDDMDCIKQVRRLLAYLPSNNLEQPPRRDMGDDPERREEALDTIIPE
                     DPTKPYDMKEVVRRIVDRGEALEVQEQYARNMITAFARMDGRTVGIVANQPMALAGTL
                     DAGSSLKAARFVRFCDSFNIPVVTLVDVPGYLPSVEQENASLLRDATKLLYAYAEATV
                     PKVTLITRKAIGGAYCAMGSKALRSDVNLAWPTAEIAIVGPEGAINIVFKQELIMADD
                     PQEKRDVLISEYRKLYASAFFSAERGYLDDIIEPNSTRMRVIEALTMLDRKREARPAK
                     KHGNMPL"
     gene            7795..9303
                     /locus_tag="PLJ11_00045"
     CDS             7795..9303
                     /locus_tag="PLJ11_00045"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/oxaloacetate carboxyltransferase"
                     /protein_id="HOO03097.1"
                     /translation="MRIKITDTVLRDAHQSLLATRMRTEDMLPAAAMLDEVGFWSVEM
                     WGGATFDTCLRFLREDPWERITKLKKEMPNTPFQMLLRGQNIVGYRHYPDDILEKFVK
                     NACRRGIDVFRIFDALNDPRNMQMAMRSVKKYGGVVEAAISYTISPVHTIDGYVAFAR
                     KLQEMGADTLCIKDMAGMLAPMDAYELVKQLREEIGLPVHLHCHATSGMVMATYMKGI
                     EAGAEIVDTAISSMSLGTSQPPTEAVVAMLRGTEYDTGLDLKRLTEISDYFQSVRRKY
                     HAFESGFTGIDTNVLVYQIPGGMISNLISQLREAKKEDRLADVYDEVPRVKADLGHVP
                     LVTPTSQIVGTQAVLNVITGERYKMVTNETKNLVRGLYGRTPAEIDPEIRRKIIGDEK
                     PITVRPADLLEPEFERLCKEIEEYACKEGVSCSEEDVLSYALFPQVALDYFKHRKMMA
                     NGVNLRDLAAVAVLYMRSTERHALAKVQGTGEADAWKIAALNEAVGRGGWPL"
     gene            9291..9803
                     /locus_tag="PLJ11_00050"
     CDS             9291..9803
                     /locus_tag="PLJ11_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOO03098.1"
                     /translation="MAAVKLRMTVNGQEYEVTVEKNGGDYRVDIGGNVYKCLVKDDKV
                     LVNGEVVPVAIEGDLEEGAKVISADRTLELKLEQVREIESFALEEPEERGKADSASGA
                     VVAPMPGKIMAVKVRPGDTVKANQVVLVLEAMKMENEVQTEVAGKVKEVKVKAGETVE
                     GGQVLLVVEP"
     gene            complement(9800..11101)
                     /locus_tag="PLJ11_00055"
     CDS             complement(9800..11101)
                     /locus_tag="PLJ11_00055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO03099.1"
                     /translation="MEQRDRDDRSGYEGIEVGSTPGGIEVIVESVPHVRTVALSVSAI
                     VGSRDERKGESGVSHFLEHMLFRGTRTRDYREVNERIEASGGYLNAYTTHEHTCFYAF
                     TIDESLAIAEDLLSDVFLNPRMDPALVDLERNIVKQEIGMHMNDPATHLRTRMAEAMF
                     KGHPLARSILGTPESLDGFDPAFLLDYHRAHYCPPRVVVCAVGNVSFGRVLDWARRFD
                     DVPGRAAPKRRRRPVMQRGVNLFDTGSDRAYIGIGVPGHPASSEMALPQTLLTTVLCG
                     GTSSRLSHRIREVEGLVYSIGMYPLTFRDCGALMTYCSTSMEQVPRLFDTFGQELRRL
                     KAEGLEEGELQRAKNGLKGSLLRSACRPDQNMSAHLMRYLERGDVQGIEGQLREINAV
                     TEEQVAKAAEGLLRSDRACVTICAGQAMRAEMVEAAEALDF"
     gene            complement(11142..11336)
                     /locus_tag="PLJ11_00060"
     CDS             complement(11142..11336)
                     /locus_tag="PLJ11_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03100.1"
                     /translation="MRAERTSERTCEACGHRFPQRWRGWNAVDVRYERCPRCGHENGY
                     ESDLYRWLRQRKGCGREHKV"
     gene            complement(11333..11914)
                     /locus_tag="PLJ11_00065"
     CDS             complement(11333..11914)
                     /locus_tag="PLJ11_00065"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOO03101.1"
                     /translation="MSGLGDIVGKEVITADAIIIGTVEGVAVDTENWKIPAVRVKVRK
                     GNEGALGLNKRLVGAHKVYVATPQVASVSDTVTLAVKKDQVSEVLVDGKRLPVTASIM
                     LHKKVVGKDGKRIGIIDNVHFDAARGWEVTKLGIKLDKDAKQALELQKGLAPASQITI
                     LTKDVKSVGDMVMLRLDLEGLKEYINKKPVSKK"
     gene            12043..12549
                     /locus_tag="PLJ11_00070"
     CDS             12043..12549
                     /locus_tag="PLJ11_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO03102.1"
                     /translation="MTLTADVQRIKVLVLDDEADFLNLCREFLARDGDLEADYVASPR
                     EAMELMASREYHAVVSDHDLPGMDGMQFLRALRSMNMHIPFIMITGHGREGLAIEALQ
                     NGADFYMEKAGSPASMFEQLLRTLKAVTCLKRKASECLQDPFRVYSEVGVLLVSNGGE
                     RVMAASAR"
     gene            complement(12546..14138)
                     /locus_tag="PLJ11_00075"
     CDS             complement(12546..14138)
                     /locus_tag="PLJ11_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOO03103.1"
                     /translation="MEGERGRGFRNQRTYETMVGAGEEAAFHSRYERALERFAQSLEG
                     RNVHESIIDGRAVATGRYVTDTSPIDTELVIGEFSIGAKREASSALAAARRAFQTWSV
                     MDLDKRLAIFRKAAEIMHGELFELAAAITFDNGKNRYEAVAEVDEAIDFISYYTEAMG
                     LRNGFQEAMPPAFPGERPAVAMRPYGVWAVLCPFNFPISISTGMLTAAIITGNTAVLK
                     PSTPAPLAVHMLYDVYERAGVPPGVINLLSGSGAVAGEALVSSEKVDGIAFTGSKEVG
                     YRLLASALRRHPIPVIAEMGGKNPAIVTEKADLARAVAGVAASAFGYSGQKCSACSRA
                     FVHELVHDEFVGALLKHLARLPMGDPRERATFMGPVIHRKAVEAYGGYVAMAAKDGIV
                     HVGGEVAKGGSARGFYAPPAVVTGLPEGHPLLKNELFLPILCVQRFTDLKDAVRRAND
                     TDYGLTAGIYSRERDEVRFFFENIEAGVCYANRARGATTGAMVGSQPFGGWKGSGSTG
                     VGSGTALYLMQFMRQQSRTLVD"
     gene            14181..17210
                     /gene="acsB"
                     /locus_tag="PLJ11_00080"
     CDS             14181..17210
                     /gene="acsB"
                     /locus_tag="PLJ11_00080"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF007078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HOO03104.1"
                     /translation="MPFAPAGTNAAIYIHCNPMVFSIIGAAPLSPIPPSSERLTADAL
                     LHMGTAKRRALCSEVARAEAGPLGGTTQRLIAALSEPDLWKVDLAACLDIVDHDIARG
                     AGVEPMAVRERREWERCGLLAPLEHVPEGQAYALLLALREALACALLGFCLDALQGAV
                     EPPAMPMGDLKEGVIRGWALDEGRGWEAFRADAPDAPWTGPGLDLRSFRAVRRWLCRG
                     LGTVSSRLPLALRTRDPVTAMGEALMGTPVVLDGATLDPFCGEMLGNMLRDLFGGCLV
                     PEEGLAREVERRRWACGLPHRYEVPAPARIGSAEIIEMGLRGAEALAALAMVGTLAAL
                     SRRGDCPVEYPETAYSLPCTLGWLGEDVATLRKLLEVLERHTGLPSGRGLPDALEAGR
                     TAMVAAEALEALRYLEGDPHAGTATVGFVPDKVLRELGLAFVDDTIPGAAVLMGMPRD
                     RGQLVATVRELQARGMLIMAVDDVVRVLQEGEVQMGLGMMLYPLGGFTQLVHALDFLV
                     RAALSFGGVQAGDAERLSAYLAKRPKAFVLHYGPLDACRTAIALAALLHHVPIVTDQL
                     VEDIPDLLFHKEPQDMLQGGLEARDIRTAVTLVDIPVPFGPAFEGETVRRPDTYFEAG
                     GGRTVSYELLRMRPEDAVRDGAITVIGPDVDRLPEGSQSPLAILVDVYGKRMQEDFEP
                     VMERRIHLYLNFAEGVWHTGQRNMNWLRLSKKAFRAGLRLEHLGRILVTKLKEEFGNI
                     VSRVAVTLITDEAEMRRRMPEALAVYQQREERMAGLTDDSVDTFYSCLMCQSFAPDHV
                     CVITPERPGLCGAINWLDARTGKEIVPTGPNQPIAKGEPEDLTKGSWKGVNEAIAALT
                     HGKIVRFCAYSLMEDPMTSCGCFECVVAMSPDMQSVIVVNREYAGMTPLGMRFSSLAG
                     NIGGGRQTPGFIGIGRRYLVSRKFISADGGFLRIAWMPSSLKEGMREELINRAKELGA
                     PDFLDKVADETVVTDAEGLMEWMIKVGHPALGMPPLL"
     gene            17222..18169
                     /locus_tag="PLJ11_00085"
     CDS             17222..18169
                     /locus_tag="PLJ11_00085"
                     /inference="COORDINATES: protein motif:HMM:NF003376.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HOO03105.1"
                     /translation="MVEVPVPKEKWTGSIGTIELGRADGRDGSRPLRLGGARGLPFLS
                     FEDPEARTVALAGWVADSLEDVPRPVQDAFGERVNDPVSWAMDWKERGADLICLHLTS
                     TNPEEGDASPERAAETAMAVLRAVGLPLIVYGSGHGEKDAKVLERVGEMAKGERLLLG
                     HAEEGAYKAAAAAAMANGHAVIGFSNLDINLAKQIVILLTDFGVGRGDVVIDPLMAAL
                     GMGLEYSYSVNERIRISALAGDSMLQVPMVCDCTPAWKAREATDDVPNGGPTLERGIW
                     WESVTALAALLSGADIVVLGHPEAMAIVRDAVRGLRGEV"
     gene            18169..19488
                     /gene="acsC"
                     /locus_tag="PLJ11_00090"
     CDS             18169..19488
                     /gene="acsC"
                     /locus_tag="PLJ11_00090"
                     /inference="COORDINATES: protein motif:HMM:NF003195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HOO03106.1"
                     /translation="MPTAIEIFKHLPKKNCGECRYPTCLAFAMQLANGKAKLDDCPHL
                     SEEGRSALASSSAPPIRSVKVGVGKGAVEMGGETELYRHDKRFFHPTAFALRVTDERD
                     EEEVDGRLAYARSLRFERVGQEMGLDLLELEYRSGDPGRFIGLVRKVADGTDLPLVLR
                     CHDPAVMAAAVAEVRERRPLLHGADAVNLEQMAEVAKTAGCPLVLCADDLGRLADLVG
                     MARKKGVEDLVLDPMPADPKETLERCTIIRRSAIRRTFRGLGYPIYLSIPEGRRGMVM
                     AATAVMKYGSLLVFEELPAAQALPLMVLRQNIYTDPQVPIQVRPDLYPVNGPGPDSPL
                     LFTTNFSLTFFTVMSDLEKSKVPVWLQVVDTEGLSVLTAYSAGKLTPESVNAALQASG
                     AKERSRAGVLIIPGMVARMSVKLNEATGLRIVVGPKESSGLPRFLRS"
     gene            19572..19772
                     /locus_tag="PLJ11_00095"
     CDS             19572..19772
                     /locus_tag="PLJ11_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03107.1"
                     /translation="MQQGPYDPNKAGPQGYQGRQQGPQQWPQQTPPNQYPSNQYQQQP
                     PQPQYQQYPPQYQQPYYPEFDS"
BASE COUNT         3414 a         6350 c         6666 g         3449 t
ORIGIN      
        1 ttgtggatga cctccctttc gggggtgtac ccgaaggaca cgttcctgca ctccacgtcc
       61 cccttcacgt ccttgaggac cagcaccttg tggtcctcgc cttccagctc tggctcgtcg
      121 aggaactcga acacgcgctc cgcggccgcg gcgacggact gcaggctggt catcgcctgc
      181 cccagctgcg agaggggctg ggtgaagagg cgcacgtaca ccatgaacgc gacgatcgtc
      241 ccaatggtga tcgccccgtc caggacccgc accgcgccga cgatgcacac ggccacgtac
      301 cccaggttgc cgatgaaccc catgatgggc atcatcatgc ccgagaggaa gagcgaccgg
      361 aacgcgctgt cgaagagcgt gtcgttcatc gaatcgaact ccctctgcgc ggccgcctcg
      421 ccgttgtacg ccttgacgat cacatgcccc gtgtacgtct cctccacata tccgttcagc
      481 ttaccgaggt ggagctgctg gcgctcgaag tatttttgcg atttggacat gatgatggct
      541 atgagcgcga accccgccag cgcggagagg acggcggccg cggtcatgac gccgtccgtg
      601 aggagcatca tcaccagcgc gccgatcagg agcgccgtgg cgctcatcag cgtcccgatg
      661 ctctggttca ttgattgccc gagggtgtcc acgtcgttcg tcacccggct caggacatcg
      721 ccatagctcg tccggtcgaa gtacctcagc ggcaggcggt tgatcttcct cgagatatcg
      781 gtgcggaggc gcttcgacag cttctgaacc acggtggtca cgatgtaccc ctggccgtag
      841 gtcatgaccg ccgccaggag gtagatcgtc actaggaacg ttccgatccc tgcgatcgcc
      901 tccatgtcca tcgttccggt catcatccct tcggcgatga ggtcggtcat ggcgcggagc
      961 ctgtccgggc cgatgacggt gaacaccgtc cctatgacca cgagcacgga ggcgaggatg
     1021 atggccggga ggtaggggcg catgtaggca agcatcctgc gccacgcctc cctgaagctt
     1081 tttgccctct gccctgggcg gccgtgcccc ctgccctctc gtcctttgcg cgttccaggg
     1141 gtcatctcct caggtcctcc tccgacagct gggaattgac tatctccctg tacacaggac
     1201 atgtgccaag gagctcgctg tgccggccga tccccaccac catcccgctg tccaggacca
     1261 cgatccggtc ggcgtccatt atcgtgccga ccctctgggc gacgattatg ctcgtcaccc
     1321 ccgccgtctc cttcttgagg gcgcttcgga gcaggcggtc cgtcctgtag tccaaggcgg
     1381 agaaggagtc gtcgaagatg tagatctccg gcctccggca gatcgccctg gcgatggaga
     1441 ggcgctgctt ctgcccgccg gagatgttaa tcccgccctg agagatcgag gcctcgtacc
     1501 ccccttccat cgcctccacg aagtccgcgg cctgcgcgat cgccaccgcc ctcttgacat
     1561 cctccagcgt gcgttcggcc gccgtgtccc cgtaggcgac gttggaggcg acggtgccgg
     1621 agaacaggac cggcctctgc ggcacgtacc cgatcttcct gcgcagcgcc cgctgcgtgt
     1681 agtcgcggac gtccaccccg tccacgcgga tgctcccctc ggtgacgtcg tacaggcgca
     1741 ggatgaggtt gacgatggtg ctcttcccgc tccccgtgga gccgatgaac gcgatggtct
     1801 cacccttcct agctgtgaag ctgacgtcct tcaagacata gtcgaccgcc ccgggatatt
     1861 tgaagccgac ccggtcgaag acgatctcgc cggcggccgg cccctccggt gcggctacgc
     1921 tcccgtcctt tatcgacggt tcggtgtcga ggacctcctc gatgcgcttg gctgccacca
     1981 tggcccgggg catgatgatg aacacgatca cgagcatcat gaaccccatg acgacctgca
     2041 tggcataggc gaggaacgtg accatctccg agaaccgcgc tatctgcgcc ccgatgccga
     2101 ccgtggcctc gatgatgatc gccccgatcc agtagatgga gagggagagg aggctcatga
     2161 ccgctgacat cacgggcatc atcagggcca tcgcccgccc tgcgaacagg ttgttggcgg
     2221 tgaggtcctc gttggccctc tcgaacttcc tttcctgata ttcctccgcg ttgaaggccc
     2281 ggatgacccg gatgcccttg agcccctccc tcatgtagcg attgacctcg tcggtgagcc
     2341 attggacctt cttgaagcga gggacgacga agaccatcag caccgagacg accgcgatca
     2401 ggacggcgat ggcgacggcg gtggccgtgg tccattgcca gctcttcccc gcgatcttga
     2461 ggatcgccca tccggcgagg atgggcgcct tgatcaccac ctggagacct atggcgaccg
     2521 ccatctgcac ctgtgtgaca tcgttggtcg cgcgggtgat caggctcgcg gtggagaacc
     2581 tgttgatctc ccctgcggag aacgcctgca ccctgctgaa ctccaggnnn nnnnnnncgc
     2641 ttcgccaggg acgccgcgat gtaggcggcc atgcagccga cggccaacgc cgtgaggagg
     2701 ctgccgagcg cgcaggcgag catcggccat ccctggtcca tcacctgggc cacggacccg
     2761 ccggtctgga tcagggtggt tatggcatac atgtagtccg ggatctccag gtccaaccat
     2821 acctggagcg tgatgagcgc cgcgcacgcg cccaccagcg cccattccct ggccttgagg
     2881 tatcttagga tcatcttgca tcaacaccgt tcgatgggct ggtccccgcg gacccgctgg
     2941 ggtccctggg gcggagttct ccgctcagct ctcgccctcc ctttccagct ctccctcgag
     3001 ctccttgatc tgcccatcca gcatggccag gttggcaagc atggccttcc ggacctcccg
     3061 tagctcctcc aatagcacct cgggaggcct ttcctcgggg gggatgaact cctccacctt
     3121 gcccttccgc ctgaggtagg cccggccgat gatctccgtc gtccgggcca tgaaaagcac
     3181 ctgggaggtc atgccggcca gggtctcgtc cagccaggcc tgcccctcgg ggctcaggga
     3241 gtagtggtcc tttccctcga tgttccgtac cgtcaggagc ccatgttcct gcagccggcg
     3301 cagagccgga tatacagccc ccgtcttcgg gacccatacc ccgtcatact cctcacgcag
     3361 gaccttgatc agctcgtacc cgtacatcgg cctctccgcc agttcgatca tcatgatcaa
     3421 ctgcgagggg ctgatgcggt tgcccttgta catgtgcggc ttgcctacca tgctgtacct
     3481 ctaggtagat atctactagg tagagcggta ctgcagaacc cttatttcaa tccatttgtc
     3541 cgcttggagg catgttttaa atcgggggag gcttttggtt ggaacatgga cctgaccttc
     3601 gaggagatcc ccgaggacct ctgggaggac tgggtatggc tcgtctcccc ggccgggctc
     3661 atgtgggcgg tggaggaggc cgcccagccc ctgctcagca ccccttacct gttgacgccc
     3721 atctacacgg tcaacatgcc caagatggtg ctgttccaca tggcctggag cggcgacttt
     3781 gacatcgacg gggatgggaa ggtgcggacc gaggaaccgt atcccccggt acggatggac
     3841 gccgacatcg ccctgagggg gctgctgttc ctgctgcgca actatccatt ggtccttcgc
     3901 tggaagctcg agcctgatag gctggcatcg cttgccccca acgtctggga cgacgtcctc
     3961 gagccgcccg aggccctttg gcatgtgccc cctgagctcg agggggagcc tgtggacttg
     4021 gaaaacattt ccatcgagtt cttcaatccc ttcatacaat cgctgcggtt caggggggtg
     4081 caccgctcgc tcatcggggt cgtttccccc atgcggagca tcgcccgggc caccgccgcc
     4141 ctcgtcccag aggcggagag cgattggagg gaggcaatgg ccatggccat tcaggagctg
     4201 gagcggcgag gcctcatcga ggcggtggac gccgggctct accgcttcac cgaacggggg
     4261 gccaggatga ccgccacgga gcctgtgtcc gactgccttt gctgccgctg ccggatcgag
     4321 gaggttgtgg agtatgagct cggcggggac gaggactgag cggcggtgct atagacgtag
     4381 gccccgcggg gatgacccaa agccccttgg gcggggaccg tggtcacaag gaatattttg
     4441 agccctgaga gggttcgagt gccaagcttt atcaggcgat acaatatttg gcgccttggt
     4501 cagcagatga agctttggga cttcggcagg gtaaaggacg ggaaggtcag gataggggac
     4561 aaggtccgca ggcggttagg cctagaggac ggaagccagg tctactccac cctgttcagg
     4621 tacgaacagg acggggcccg ggggcacgag atcgtcctgt ccacgttcgg cccggagaac
     4681 tatcgggagc tctgcaacct caccttctac atgatcgacg ccccgggggc ctgcgcccag
     4741 gccgccaagt tcctcgcgga ccgcaacgtg gacatattga actcggtctc ggtcagcatg
     4801 acctgcggca tctccatggt ctggaggatg ctggtggacc tctcctacta cggggactcg
     4861 acgcagctca tggaggactt cgcctcggtg agacgggcca gcccggccgc cctctccaag
     4921 gtggaggggc tggagatcag ccattcccat atcagcgacc gctacaccaa gggcatgacc
     4981 ggcccgcagc ccgggcgcat caaggtcaag ccgctgcgca aggtggcccg ctccccttcc
     5041 cttatcaagg acgagagctt caccattccc aaggactacc tggagcaatt ggagggcgtg
     5101 agggacggca cccccgtcat gatggtgggg gacgtggact cctgggtcct ctccatatcc
     5161 tttttgccgg aggactgcca tctggccgag ttctccttcg agatcccgga caggcccggc
     5221 tcgatctaca ggaccacgga cgccctagcc actcagggcg tcaacctgct ggcggtgcag
     5281 tccaaggtgc tggtctacgg gcagaggatg acgttggacg tcgtggccga cctgaaggac
     5341 gcgggcgagc tggacaaggt cgtggagagg acgctcatgg ccctgggcgg ggagtacagg
     5401 ctccgctgca aggggccggt gaccgtctga gggctgcctg atgctcatag gccgaaaggt
     5461 gctggtcaag gacgaggtag attccaccaa cgacgtcgcc aaggccttgg ccgtcgaagg
     5521 ggagccggag ggcacggtgg tcaccgccgc caggcagacc tgcggccggg gccggaccgg
     5581 ccggaggtgg gagtccccgc ggggcggcct ctacctttct gtcatccttc ggccggagct
     5641 gccggccccg gacctcactt tgttcacggt cctctcctgc cttccggtgg cccaggccat
     5701 ggaggaggtg tgccgcgtgg tgcccagcat caaatggccg aacgacgtgc gggtcgaagg
     5761 gaagaaggtc gcaggcatcc tcggggaggt ctgctatcgg ggcggggagc cgaggttcat
     5821 ggtactgggc atcggcatcg acctcaacac cgacccggcg gcgctgaccg cgccggaggc
     5881 gggcagcctc agggagatct gcaaaaggga cctggacccc gagcgcttcc ttaacgtcct
     5941 cctgttcagg ctggacgaca tgtacgccag gttcaggaag ggggagcggg agctggacct
     6001 ctacgcgcgg tactccgagt ccatcggaag ggacgtcgag gtgcgcctgg gggaccagcg
     6061 ggtgcgggga aggggcatgc gcctggacgc cgacggcgcc ctggtgctcc gctccgagga
     6121 cgggatgatc tatcgggcga cctcggtgca cgacgtcgtt cccttggacc gggggcaggt
     6181 gaggttcgag ggcgggtaac gttaattagc tatgagcacg aataggagag gaacagcatg
     6241 ggcgaggaca ggatcaagga actccgggag atgaagaagc ggtcccgggc gggcggcggg
     6301 gcggagaggg tcgaggccca ccacaaacag ggcaagatga cggcccgcga acgggtggac
     6361 gccctcctgg acccgggttc gttctccgag ctggacacgt tcgtatcgca tcaggcgtac
     6421 catttcggca tggacaagca gaaggtccca ggggacgggg tggtgaccgg gcgcggcacc
     6481 atcgagggaa ggcaggtgtt cgtctacgcc caggacttca ccgtcttcgc cggctccgtg
     6541 ggacggatgc acgccacgaa gatctgcaag gtcatcgacc tggcggtgaa gacgggctgt
     6601 ccggtgatcg ccctctacga ttccggcggg gcgaggctgc aggagggggt cgactccctg
     6661 gccggctatg gcgaggtcat gctgcgccac acgctcgcct cgggcctcat cccccagatc
     6721 gccatggtca tgggcccctg cgccggtgcc ggcgcctacg ccccgatcct cagcgacttc
     6781 gtgttcatgg tcaaggacaa gtccttcatg ttcatggtag gcccggacgt catcaaggcc
     6841 gtccagaacg agacggtgac cttcgaggag ctcggcgggg ccagcgtcca cgccagccgc
     6901 tccgggtccg cgcatttcgt ggccaaggac gacatggact gcatcaagca ggtcaggagg
     6961 ctgctggcgt acctgcccag caacaacctc gagcagccgc cgaggaggga catgggcgat
     7021 gaccccgagc ggcgggagga ggccctggac accatcatcc ccgaggaccc cacgaagccg
     7081 tacgacatga aggaggtcgt gcgcaggatc gtggacaggg gcgaggccct cgaggtccag
     7141 gagcagtacg cgcgcaacat gatcaccgcc ttcgcccgga tggacgggag gacggtgggc
     7201 atcgtggcga accagcccat ggcgttggcg ggaacgctcg acgccggctc ctccctcaag
     7261 gccgcccgct tcgtgcgctt ctgcgattcg ttcaacatcc ccgtggtcac cctggtggac
     7321 gtcccgggct acctgcccag cgtggagcag gagaacgcct ccctgctgag ggacgcgacc
     7381 aagctgctct acgcgtacgc ggaggcgacc gtcccgaagg tcacgctcat cacccggaag
     7441 gccatagggg gcgcgtactg cgccatgggc tccaaggccc tccgctccga cgtgaacctg
     7501 gcgtggccga ccgcggagat cgccatcgtg ggcccggagg gggccataaa tatcgtgttc
     7561 aagcaggagc tgatcatggc cgatgacccc caggagaagc gggacgtgct gatctcggag
     7621 taccggaagc tgtacgcctc ggccttcttc tccgctgagc gcgggtattt ggacgacatc
     7681 atcgaaccga acagcacgcg catgcgcgtg atcgaggcgc tgaccatgct cgaccgcaag
     7741 cgcgaggccc gtccggccaa gaagcatggc aacatgccgc tctgaggtgc catcatgagg
     7801 atcaagatca cggacaccgt actgagggac gctcatcagt ccctcctggc gaccaggatg
     7861 cgcaccgagg acatgctccc ggcggcggca atgctggacg aggtcggctt ctggtcggtg
     7921 gagatgtggg ggggcgcgac gttcgacacc tgcctgcgct tcctgaggga ggacccttgg
     7981 gagcgcatca ccaagctgaa gaaggagatg cccaacactc ccttccagat gctgctccgc
     8041 ggccagaaca tcgtcggcta caggcactac ccggacgaca tcctggagaa gttcgtcaag
     8101 aacgcctgcc gccggggcat cgacgtcttc cgcatcttcg acgcgctcaa cgacccgcgc
     8161 aacatgcaga tggccatgcg ctcggtcaag aagtacggcg gggtggtgga ggcggccatc
     8221 tcctatacca tctccccggt gcacaccatc gacgggtatg tcgccttcgc ccgcaagctg
     8281 caggagatgg gagcggacac gctctgcatc aaggacatgg cggggatgct ggcgcccatg
     8341 gacgcctacg agctcgtgaa gcagctccgg gaggagatcg ggctgccggt gcatctgcac
     8401 tgccatgcca cctccggcat ggtcatggcc acatacatga aggggataga ggcgggggcg
     8461 gagatcgtgg acaccgcgat ctccagcatg tccctgggca cctcccagcc cccgacggag
     8521 gccgtggtgg cgatgctgcg gggcacggag tacgacaccg ggctggacct caagcggctg
     8581 accgagatct ccgactattt ccagtcggtg cggaggaagt accacgcctt cgagagcggg
     8641 ttcaccggca tcgacaccaa tgtgctggtg taccagatcc cggggggaat gatctccaac
     8701 ctcatctccc agctgcggga ggcgaagaag gaggaccggc tggcggacgt gtacgatgag
     8761 gtgccgaggg tgaaggccga cctcgggcac gtgcccctgg tcaccccgac ctcgcagatc
     8821 gtggggacgc aggccgtgct gaacgtcatc accggggaaa gatacaagat ggtcaccaac
     8881 gagacgaaga acctggtccg agggctgtac ggcaggacgc cggcggagat cgatcccgag
     8941 atccgccgga agatcattgg ggacgagaag cccatcacgg tgcgtcccgc cgatctcctg
     9001 gagccggagt tcgaaaggct gtgcaaggag atcgaggagt acgcgtgcaa ggagggcgtg
     9061 agctgctcgg aggaggacgt gctctcctac gccctcttcc cccaggtcgc gcttgactac
     9121 ttcaagcacc gcaagatgat ggcgaacggg gtgaacctcc gggacctggc cgcggtggcc
     9181 gtgctgtaca tgcgctccac ggagaggcac gccctcgcca aggtccaggg gacgggcgag
     9241 gccgatgcct ggaagatcgc ggccctgaac gaggcggtcg ggcgcggggg gtggccgctg
     9301 tgaagctgcg catgacggtg aacgggcagg agtacgaggt gacggtcgag aagaacggcg
     9361 gggactaccg tgtggacatc gggggcaatg tctacaagtg cctcgtcaag gacgacaagg
     9421 tgctcgtgaa cggcgaggtc gtcccggtgg ccatcgaggg ggacctggag gagggggcca
     9481 aggtcatctc cgcggatcgc acgttggagc tcaagctgga gcaggtgagg gagatcgaga
     9541 gcttcgccct ggaggagccc gaggagaggg ggaaggccga cagcgcctcg ggggccgtgg
     9601 tcgcgcccat gcccggcaag attatggccg tcaaggtgag gccgggggac accgtcaagg
     9661 ccaatcaggt ggtcctcgtc ctggaggcca tgaagatgga gaacgaggtt cagaccgagg
     9721 tggccgggaa ggtcaaggag gtcaaggtca aggccgggga gaccgtcgag ggcggccagg
     9781 tgctcctcgt cgtggagcct tagaagtcca gggcctcggc ggcctccacc atctccgccc
     9841 tcatcgcctg gccggcgcag atggtgacgc acgccctgtc gctgcgcagc agcccctcgg
     9901 ccgccttggc gacctgctcc tccgtcaccg cgtttatctc ccggagctgt ccctctatgc
     9961 cttgcacatc gcccctttcc aggtagcgca tcaggtgcgc gctcatgttc tggtccgggc
    10021 ggcaggcgct ccgcagcagc gagcccttca gcccattctt cgcccgttgc agctccccct
    10081 cctccagtcc ctctgccttg aggcgacgga gctcctggcc aaaagtgtcg aataggcggg
    10141 ggacctgctc cattgatgtg gagcagtagg tcatcagcgc cccgcagtcc ctgaaggtga
    10201 gggggtacat gcctatcgag tataccagcc cctccacctc cctgatgcgg tggctgaggc
    10261 gggaggaggt cccgccgcac agcaccgtgg tcagcagcgt ctgcgggagc gccatctcac
    10321 tggaggcggg gtgcccgggg acccctatgc cgatgtacgc tctgtcggaa ccggtatcga
    10381 agaggttgac gcccctctgc atgaccggcc tccggcgccg cttgggcgct gccctgccgg
    10441 gcacatcgtc gaacctgcgg gcccagtcga gcacgcggcc gaagctcacg ttgcccacgg
    10501 cgcacaccac cacgcgcgga gggcagtagt gggcgcgatg atagtccagt aggaaggccg
    10561 ggtcgaagcc gtccaggctc tcgggggtgc cgaggatgga gcgcgcgagc ggatggccct
    10621 tgaacatggc ctcggccatc cgtgtgcgta ggtgagtggc cgggtcgttc atgtgcatcc
    10681 cgatctcctg cttcacgatg ttccgttcca ggtccacaag ggcagggtcc atcctggggt
    10741 tcaggaacac gtcggagagc aggtcctccg cgatggctag cgactcgtcg atggtgaacg
    10801 cgtagaagca ggtgtgctca tgcgtggtgt acgcgttcaa gtacccgccc gaggcctcga
    10861 tcctttcgtt cacctcccgg tagtccctcg tgcgggtccc ccggaacagc atgtgctcga
    10921 ggaagtgcga cacgccgctc tccccctttc gctcgtcgcg ggagcctacg atggcggaga
    10981 cggagagcgc gaccgtgcgg acgtggggga cgctctccac gatgacctcg atgcctccag
    11041 gcgtgctccc gacctctatg ccctcgtatc ccgaacggtc gtccctatcc cgctgttcca
    11101 tgggggcggg catatgccct ctaagagata ggcttgtcgc ctcagacctt atgctcccgc
    11161 ccgcacccct tcctctggcg gagccagcgg tacaggtcgg actcgtagcc gttctcgtgc
    11221 ccgcaccggg ggcatcgttc gtacctgaca tccacggcgt tccagccccg ccatcgctga
    11281 gggaagcggt gcccgcatgc ctcgcacgtg cgctcggacg tgcgctcggc tctcatttct
    11341 tggacacggg cttcttgttg atgtactcct tcaacccctc caagtcgagg cggagcatga
    11401 ccatgtcgcc cacgctcttg acgtccttgg tcaggatggt gatctgcgag gccggggcca
    11461 gtcccttctg gagctccaac gcctgcttcg cgtccttgtc cagcttgatc cccagcttgg
    11521 tgacctccca gccccgggcg gcatcgaaat ggacgttgtc tatgatcccg atccgcttgc
    11581 cgtccttgcc caccaccttc ttgtggagca tgatggacgc tgtcaccggc agcctcttgc
    11641 catccaccag gacctcgctg acctggtcct tcttgaccgc caaggtgacg gtgtcgctga
    11701 cggaggccac ctgaggcgtg gcgacgtaga ccttgtgcgc gccgaccagc cgcttgttca
    11761 ggcccaaggc gccctcgttg cccttccgca ccttgaccct gaccgccggt atcttccaat
    11821 tctcggtgtc cactgccacg ccctccaccg ttcctattat gatagcgtcg gcggtgatga
    11881 cctccttgcc cactatgtcc cctaaccctg acatgttgtc ccctgcgcta cacctcctag
    11941 aattatatta attctatcgt cttgggaagc ggatcgcgaa gctgataaag ggcaccgtag
    12001 gttaaaatag caccgcagcc cgtattgggg cggatggagc gcatgaccct gaccgcagac
    12061 gttcaacgga tcaaggtgct ggtgctggac gatgaggctg atttcctgaa cctttgccgg
    12121 gagttcctcg cccgggacgg ggacctggag gctgattatg tcgcgtcccc gcgggaggcc
    12181 atggagctga tggcctcgag ggagtaccat gcggttgtgt ccgaccatga cctgcctggc
    12241 atggatggaa tgcagttcct ccgagccctg cggtcgatga acatgcacat ccccttcatc
    12301 atgatcaccg gccatggccg ggaagggctg gccattgagg cgctgcagaa cggcgctgac
    12361 ttctacatgg agaaggccgg cagcccggcc agtatgttcg agcagctcct gcgcaccctg
    12421 aaggcggtga cctgcttgaa gaggaaggcc tccgagtgcc tccaggatcc gttccgggtc
    12481 tacagcgagg tgggggtgct gctggtcagc aacggggggg agcgggtcat ggccgcctcc
    12541 gcgcgctagt cgacgagcgt cctgctctgc tgccgcatga actgcatcag atagagggcg
    12601 gtcccgctgc ccacgcccgt ggagccgctg cccttccagc ccccgaacgg ctgggatccg
    12661 accatagccc cggtggtggc ccccctggcc cggttggcgt agcacacccc ggcctctatg
    12721 ttctcgaaga agaaccgcac ctcgtccctt tcccgggagt agatcccggc cgtcaggccg
    12781 tagtcggtgt cgttcgcccg gcgcaccgca tccttcaggt cggtgaagcg ctgcacgcag
    12841 aggatgggga ggaagagttc gttctttaac aacgggtggc cttcgggaag cccggtcact
    12901 acggcgggcg gggcatagaa ccccctggcc gatccaccct tcgcgacctc cccgccgaca
    12961 tggacgatgc cgtccttggc ggccatggcc acatatcccc cgtaggcctc cacggccttg
    13021 cggtggatga ccgggcccat gaaggtggcc cgctcccggg ggtcccccat aggcagccgc
    13081 gccaggtgct tgaggagcgc ccccacgaac tcgtcgtgga ccagctcgtg cacgaacgcc
    13141 cgggagcagg cgctgcactt ctgcccggaa tagccgaagg cggaggcggc cactccggcg
    13201 acggcgcggg ccaggtcggc cttctcggtg actatggcgg ggttcttgcc tcccatctcg
    13261 gcgatgaccg ggatgggatg gcggcgcagc gccgacgcaa gcagcctgta ccccacctcc
    13321 ttggagccgg tgaacgctat cccgtccacc ttctcgctcg agacgagggc ttccccggcc
    13381 accgccccgc tgcccgacag gaggttgatg accccgggcg gcacccccgc cctctcgtac
    13441 acgtcgtaga gcatgtgcac ggccaggggg gcgggcgtgg agggcttgag cacggccgtg
    13501 ttcccggtga tgatggccgc ggtgagcatc ccggtcgata tggagatggg gaagttgaac
    13561 gggcacagca ccgcccacac accatagggg cgcatggcga cggccggccg ctcacctggg
    13621 aacgctggcg gcatggcctc ctggaacccg ttccgcaatc ccatcgcctc ggtgtaatag
    13681 gagatgaagt cgatcgcctc gtccacctcc gccaccgcct cgtagcggtt cttcccgttg
    13741 tcgaaggtga tcgcggccgc gagctcgaag agttcgccat gcattatctc cgccgccttg
    13801 cggaagatcg ccaaacgctt gtccaggtcc atcactgacc aggtttggaa ggcccggcgg
    13861 gccgcagcca gcgcggagga ggcctccctc ttcgcgccta tcgagaactc tccgatcacc
    13921 agttcggtat cgatagggct cgtgtccgtg acatacctcc cggtcgccac cgcccttccg
    13981 tcgatgatgc tttcgtgaac attcctccct tccagggatt gtgcgaacct ttccagcgcc
    14041 cgctcgtacc ggctgtggaa cgcggcctct tcccccgctc cgaccatggt ctcataggtg
    14101 cgctggttcc gaaagcccct tccccgctct ccttccatgg atgaatatag gggggagggg
    14161 cgcctataag ggcatcgccg atgcccttcg ccccggccgg aacgaacgcc gcaatctata
    14221 tccactgcaa tccgatggtc ttctccatca taggtgctgc tcccttgtcg ccgatccccc
    14281 cgtcctcaga gcgcctgacc gccgatgcct tgctgcatat gggaaccgcc aagcgccgcg
    14341 ccctatgctc agaggtcgcg agggccgagg cggggccgct aggggggacg acccaaagat
    14401 tgatcgccgc cctttccgag cccgacctct ggaaggtgga cctggcagcc tgcctggaca
    14461 tcgtcgacca tgacatcgcc aggggggccg gcgtggaacc gatggccgtg cgcgagcgcc
    14521 gggagtggga gagatgcggg ctcctcgctc cactggagca cgtccctgag gggcaggctt
    14581 acgccctcct cctcgccctc agggaggccc tggcctgcgc gctcctgggc ttctgcctgg
    14641 atgccctgca gggagcggtc gagcctccgg ccatgcccat gggcgacctg aaggaggggg
    14701 tgatcagggg atgggcattg gacgagggga gaggctggga ggccttccgc gccgatgccc
    14761 ccgatgcccc gtggacgggg ccgggtctcg accttcgctc gttccgagcg gtgcgccgat
    14821 ggctgtgccg gggcctgggc acggtgtcct cccggttgcc cctagcccta cgcacgaggg
    14881 acccggttac ggccatgggc gaagcgctga tggggacgcc ggtggtcctc gacggggcga
    14941 ccctggaccc tttctgcggt gagatgctcg gcaatatgct ccgggacctc ttcggcggct
    15001 gcctggtccc cgaggagggg ctggcgaggg aggtggagcg gaggcgctgg gcctgcggcc
    15061 tgccgcatcg gtacgaggtg cctgcgccgg cgaggatcgg gagcgctgag atcatagaga
    15121 tggggctcag gggggcggag gccctcgcag ccctggcaat ggtcgggacc ctcgccgccc
    15181 tctcgcgccg gggggactgc cccgtggagt acccggagac cgcctactcc ctgccgtgca
    15241 ccttgggatg gctcggggag gacgtcgcca ccctccggaa gctgttggag gtgctggagc
    15301 gccataccgg tcttcccagc gggagggggc tgcctgacgc gctggaggcg ggcaggacgg
    15361 cgatggtggc cgccgaggcc ctggaggcct tgaggtacct tgaaggggac ccgcacgccg
    15421 gcacggccac cgtaggcttc gtcccggaca aggtgctcag ggagctgggg ctggcctttg
    15481 tggacgacac catccccggg gccgcggtgc tgatgggcat gccccgggac cgcgggcagc
    15541 tggtggccac tgtccgcgag ctgcaggcca ggggaatgct gatcatggcc gtggatgatg
    15601 tggtaagggt gctccaggaa ggggaggtcc agatggggct tgggatgatg ctctaccccc
    15661 tcgggggctt cacccagctg gtgcatgccc tggatttcct ggtcagggcg gcgctctcgt
    15721 tcggaggggt ccaggcaggg gacgccgaac ggctgtccgc gtacctggcc aagcgcccca
    15781 aggccttcgt gctgcactat ggacctctgg acgcgtgccg gacggccata gccctggccg
    15841 cgctcctcca ccacgtgccg atcgttaccg accagctggt ggaggacatt cctgacctgc
    15901 tgttccacaa ggagccgcag gacatgctcc agggcgggct ggaggcgcgg gacatccgca
    15961 ctgcggtgac gctggtggac atccccgtcc cgttcggccc ggccttcgag ggcgagacgg
    16021 tgaggcgtcc ggacacctac ttcgaggcgg ggggagggcg caccgtgtcc tacgagctgc
    16081 tgcgcatgcg cccagaggat gcggtcaggg acggcgccat caccgtcatc ggcccggacg
    16141 tggaccgcct gccggagggc tcgcagtccc ccttggccat cctcgtggac gtctacggga
    16201 agaggatgca ggaggacttc gagccggtga tggagcgccg catccacctc tacctgaact
    16261 tcgcggaggg cgtctggcac acggggcagc gcaacatgaa ctggctgcgg ctgagcaaga
    16321 aggccttccg cgccgggctc cgcctggagc acctgggccg gatcctggtg accaagctca
    16381 aggaggaatt cgggaacatc gtgtcgcggg tggcggtgac gctcatcacc gacgaggccg
    16441 agatgaggag aaggatgccg gaggcgctcg ccgtctacca gcagcgggag gagcgcatgg
    16501 ccgggctcac cgatgacagc gtggacacgt tctattcctg cctgatgtgc caatccttcg
    16561 ccccggacca cgtgtgcgtc atcaccccgg agcgcccggg actgtgcggg gcgatcaact
    16621 ggctggatgc caggaccggg aaggagatcg tgcccaccgg ccccaaccag ccgatagcca
    16681 agggcgagcc ggaggacctg acgaaggggt cctggaaggg ggtcaacgag gcgatcgccg
    16741 ccctcaccca tgggaagatc gtgcggttct gcgcctatag cctgatggag gacccgatga
    16801 cctcctgcgg ctgcttcgag tgcgtggtgg ccatgagccc ggacatgcag tcggtcatag
    16861 tggtgaacag ggaatatgcg ggcatgaccc cgttggggat gcgcttctcc agccttgccg
    16921 ggaacatcgg cggggggagg cagacgccgg ggttcatcgg catcgggcgg aggtacctcg
    16981 tcagcaggaa gttcatctct gcggacggcg gcttcctgcg catagcctgg atgccctcct
    17041 cgctcaagga gggcatgcgg gaggagctta ttaatagggc caaggaattg ggagcgccgg
    17101 acttcctgga caaggtggcc gacgaaacgg tcgtcaccga tgccgagggt ctgatggagt
    17161 ggatgatcaa ggtgggccat cccgccctgg gaatgccccc gctgctctga ggtggtagtg
    17221 aatggtcgag gtgccggtgc cgaaggagaa atggacggga tcgataggaa cgatcgagct
    17281 ggggcgcgcc gatgggaggg atggctcccg cccattgcgg ctcgggggag cccgagggct
    17341 gccgttcctc tcgttcgagg acccggaggc ccggacggtg gccctggccg ggtgggtggc
    17401 cgattccctc gaggacgtcc ctcgaccggt ccaggacgcc ttcggcgaga gggtgaacga
    17461 cccggtgagc tgggcgatgg attggaagga gcggggcgcg gacctcatct gcctacacct
    17521 tacctccacc aatccagagg agggggatgc ctccccggag cgcgccgccg agacggccat
    17581 ggcagtgctg cgggcggtcg gccttcccct gatagtctac ggcagcgggc acggggagaa
    17641 ggacgccaag gtgctggaga gggtggggga gatggccaag ggggagcgcc tcctgctcgg
    17701 gcatgcggag gagggcgcct acaaggcggc cgcggccgcg gcgatggcca acgggcatgc
    17761 ggtgataggg ttctcgaacc tggacatcaa cctcgccaag cagatagtga tcctgctcac
    17821 cgacttcggc gttgggcggg gcgacgtcgt gatcgacccg cttatggccg ccctggggat
    17881 ggggctcgag tactcttact cggtgaacga gcggatccgg atctccgccc tggcgggcga
    17941 cagcatgctc caggtgccga tggtctgcga ctgcaccccg gcctggaagg cgcgggaggc
    18001 gaccgacgac gtgccgaacg ggggcccgac ccttgaaagg gggatatggt gggagagcgt
    18061 caccgccctg gcggccctgc tctccggggc cgacatagtg gtgctggggc acccggaggc
    18121 gatggcgatc gtcagggacg cggtcagagg gctgaggggg gaggtctgat gcccaccgcc
    18181 atcgagatat tcaagcacct ccccaagaag aactgcgggg agtgcaggta cccgacatgc
    18241 ctggccttcg ccatgcagct ggccaacggc aaggccaagc tggacgactg cccccacctc
    18301 agcgaggagg ggaggagcgc cctggcaagc tcctccgccc ctcccatccg ctcggtcaag
    18361 gtgggcgtgg gcaagggcgc ggtggagatg gggggcgaga ccgagctgta ccggcacgac
    18421 aagcgcttct tccacccgac ggcgttcgcc ctccgggtca cggacgagag ggacgaggag
    18481 gaggtggacg gaaggctggc ctatgcccgg tccctgcgct tcgagcgtgt cgggcaggaa
    18541 atggggctgg acctccttga gctggaatac cgctctggcg atccgggccg gttcatcggg
    18601 ctcgtgagga aggtagcgga cgggacggac ctgccattgg tgctgcgctg tcatgacccg
    18661 gccgtcatgg ccgccgccgt ggcggaggtc agggagcgcc ggccgctgct gcatggcgcg
    18721 gacgccgtca acctggagca gatggccgag gtggccaaga ccgctggctg cccccttgtg
    18781 ctctgcgccg atgaccttgg aaggctggcc gacctggtgg ggatggcccg gaagaagggg
    18841 gtcgaggacc tcgtcctgga ccccatgccc gccgatccga aggagacgct ggagcggtgc
    18901 accatcatca ggcgcagcgc catccgcagg accttccgcg gcctcgggta tccgatctac
    18961 ctttccattc cggaggggcg caggggcatg gtcatggccg ccacggccgt catgaagtac
    19021 gggagcctgc tggtgttcga agagcttccg gcggcccagg cgcttccgct catggtcctt
    19081 cgccagaaca tctataccga tccccaggtt cccatccagg tcaggccgga cctctatccg
    19141 gtcaacggcc ccgggccgga ctcccccctc ctcttcacca ccaacttctc tctgaccttc
    19201 ttcaccgtca tgtccgacct tgagaagagc aaggtgcctg tctggctgca ggtggtggac
    19261 acggaagggc tctcggtgct gaccgcttat tcggcaggga agctgacgcc ggaatcggtc
    19321 aatgccgccc tgcaggccag cggagcgaag gagcggtcga gggcgggcgt gctcatcatc
    19381 ccgggcatgg tggccaggat gagcgtcaag ctgaacgagg ccaccgggtt gcggatcgtg
    19441 gtcgggccga aggaatcctc aggcctgcct aggttcctgc gctcctgagg tgttatcggc
    19501 ccctgccaag cgcttgaaaa gcattatcat ccgcctgaat gttgtcttcc taacatccaa
    19561 ggtggaatcg gatgcagcaa ggaccctatg acccgaacaa ggccggcccg cagggatacc
    19621 agggccggca gcaggggccc cagcaatggc ctcagcaaac ccctccgaac caatatcctt
    19681 cgaaccaata ccagcagcaa ccgccgcagc cgcagtacca gcagtacccg ccgcagtacc
    19741 agcagccgta ctaccctgag ttcgacagtt gaccgggcaa aatttcactg attttagatg
    19801 ttgacagttg gtccatgagg ctccgaacct tgttgaaaac gcccgttccc gggcgttttc
    19861 cgctacgttt cggatagaaa atcggccaa
//
LOCUS       DAOSYN010000002        18829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2432_length_18829_cov_4.305795, whole genome shotgun sequence.
ACCESSION   DAOSYN010000002 DAOSYN010000000
VERSION     DAOSYN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 18829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18829
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2432_length_18829_cov_4.305795"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /gene="tgtA"
                     /locus_tag="PLJ11_00100"
     CDS             complement(<1..1112)
                     /gene="tgtA"
                     /locus_tag="PLJ11_00100"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HOO03108.1"
                     /translation="MLELVRRDGLARICRLHTRQGELETPCLLPVINPRLVTISPREL
                     YDVFGFRALITNSYIIRNDQRLREEALEGGVHAMLDYPGIIMTDSGTFQSHMYGDVDL
                     INRDIVEFQRDIGSDIGTVLDIFAEPDWSKERTAGSLEVTLERTMEAAGLKGEMLLAG
                     VVQGSIYPDLREMCARRLSEMEVDVNPIGGVVPLMENYRFAELVDVIVAAKKGLRPDR
                     PVHLFGAGHPMLFALAALLGCDMFDSASYAKFARDDRFMFLEGTFRLQDMKALECQCP
                     ACFGHGLEEIRGMPPAQRTALIARHNLWISKTELDRVRRAILEGDLWELVERRCRSHP
                     ALLDALRALERHQGFLEGFEPLSREHALFYTVVAVS"
     gene            1189..1425
                     /locus_tag="PLJ11_00105"
     CDS             1189..1425
                     /locus_tag="PLJ11_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03109.1"
                     /translation="MAYLDAMESLVMCCPRCDILITVMVQDCLEGREFPCPICGEVIA
                     FKFTPEEMEQKLEMAKKNQERALRRQFPLTFGDV"
     gene            1432..1791
                     /gene="pth2"
                     /locus_tag="PLJ11_00110"
     CDS             1432..1791
                     /gene="pth2"
                     /locus_tag="PLJ11_00110"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HOO03110.1"
                     /translation="MEFELKMVIVVRKDLKLSPGKMAAQVAHAAVNCALASKKRRPIN
                     FDRWYSEGQKKVVLKAQDLNELIQIKQAAEDAGLVTSLITDAGLTELPPNTTTCLGIG
                     PAPNAEMDRITGHLGLM"
     gene            1792..2364
                     /locus_tag="PLJ11_00115"
     CDS             1792..2364
                     /locus_tag="PLJ11_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HOO03111.1"
                     /translation="MKDQARVLGIDDGPFIKGQARAPLAGVLVCPPGYVEGILVSSCQ
                     VDGEDANEAIVEMVRDSRFFEQVRMVMVDGAALGGFNVVDIQGLSAELGIPVLTVSRD
                     EPNMRSISSALRAHFSDWERRLEVISRSAIRTVEVPDGRVFVASAGIGEKEADAMVRR
                     CTVRGCLPEPIRLAHLVATALVRGESKGRA"
     gene            2404..2814
                     /locus_tag="PLJ11_00120"
     CDS             2404..2814
                     /locus_tag="PLJ11_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03112.1"
                     /translation="MDDLISKVAEERLRAIQRELPPAEVVTHFHEDERGRGLIDIVYQ
                     GALVGQEIIETHDSWKEEGRLAAYQAVLRKKVRLVVMAPRGEAMRVRYMMLELNNCWL
                     FYYMVYSYDPDGHLMRVLRPAVPGRREPAVPYIQ"
     gene            2937..3374
                     /locus_tag="PLJ11_00125"
     CDS             2937..3374
                     /locus_tag="PLJ11_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOO03113.1"
                     /translation="MTHLAKGLFTARKLRNDRQKARWSDRSYKRRLLKLKERSDPLEG
                     AAQAKGIVLEKVGIEAKQPNSAIRKCVKVQLIKNGRQITAFAVGDGAINFIDEHDEVL
                     VEGIGGRLGRSYGDIPGVRYKVIKVNNVSLNELVRGRKEKPVR"
     gene            3380..3967
                     /locus_tag="PLJ11_00130"
     CDS             3380..3967
                     /locus_tag="PLJ11_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOO03114.1"
                     /translation="MEEGSFKFDNILLFGKYSSGDVVVNDGGLAKYINLTPTAVPHTT
                     ARHANKWFGKSKVNIVERLINNMMRTEVFTGKKLKAYNVTKKAFEIIEQRTKRNPIQV
                     LVEALENAAPREEITRLQFGGISVPKAVDIAPSRRLDIALRNITKGTVNASFKNTKPI
                     EQCLAEELILASKGDMNSFSVAKKEEIERVASSAR"
     gene            3982..6180
                     /locus_tag="PLJ11_00135"
     CDS             3982..6180
                     /locus_tag="PLJ11_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HOO03115.1"
                     /translation="MGRKEDNIRRAQEIMLKPEFIRNIGTAAHIDHGKTTLSDNLIAG
                     AGMMSENLAGKQLMLDFDEQEQARGITINAANASMVHHWKGNDYLINLIDTPGHVDFG
                     GDVTRAMRALDGCIILVCAVEGIMPQTETVIRQALKERVRPVLFVNKVDRLINELKVT
                     EQEMQQRFVQIISEVNKRIAANLPEDLRKKWQVKVEDGSVAFGSAFNNWAISAPYMKK
                     SGISFKDVYNYCKNEDQKTLAKKSPVHEVLLEMVITHVPSPLEAQKVRIPTIWKGDLN
                     SEVGRAMMTCDRNGPTTFMCTKIIIDPHAGEVAVGRLFSGKVVRGQELYVLGMPNPNR
                     VQTVALSVGADRIAVDEVEAGNIVALTGLKDAIAGATCSSEKDMEPFEKITDYTEPVV
                     TVAIEAKHMKDLPKLVDVLRTIGKADPSIQVQINQETGENLLSGMGELHLEITQYRIV
                     NDHKVEIKASAPIVVYRESIKGRGGPFEGKSPNKHNKFYIMVEPLEEAVIEAIKNGEI
                     KTEGKIKDPKALAKQLSDLGMDKEQAKGVVAFKNNNVFIDVTKGIQNLHETIELCKQA
                     FEEAMEMGPLAGERVSGVKVLLVDAKLHEDSIHRGPGQVIPATRSGIYGAMCMAGRVL
                     LEPIQKVYINCPQDMMGDVTRELQGRRATIEDIRQEHDATVVVATAPVAEMFGFASAI
                     RGATQGRALWSTENSGFVILPRELQDKVVGEIRKRKGLNPQPYDAAYYSG"
     gene            6264..7541
                     /gene="tuf"
                     /locus_tag="PLJ11_00140"
     CDS             6264..7541
                     /gene="tuf"
                     /locus_tag="PLJ11_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HOO03116.1"
                     /translation="MADKPHMNLVFIGHVDHGKSTTVGRMLLDTGAIDPYVIEKYRKQ
                     AEEKGKATFEFAWVMDSLKEERERGVTIDVSHKRFDTDKYYFTVIDAPGHRDFVKNMI
                     TGTSQADAAVVVVSAIEGPQAQTREHIFLAKTLGVKQIIIAVNKMDAVKPPYDEAAYK
                     KTVNEVSSLLKTVGFKVEEIPFIPISAFKGDNCGKRSENMPWYKGDTLLEALNKLKVP
                     QSTEGLPLRLPIQDVYTITGIGTVPVGRVETGVLKPDMKVIFMPSNKVGEVKSIEMHH
                     ETLPVAKPGDNVGFNVRGIAKNDIKRGDVAGPVDNPPTVAKTFTAQIVVLNHPTAIPV
                     GYTPVFHCHTAQVACTFIELQKKLDPRTGQVKEENPTFLKTGDVAVVKVMPTKPLVIE
                     RAKDFPQLGRFAIRDMGQTVAAGMCIDVEKKEM"
     gene            7576..7887
                     /gene="rpsJ"
                     /locus_tag="PLJ11_00145"
     CDS             7576..7887
                     /gene="rpsJ"
                     /locus_tag="PLJ11_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOO03117.1"
                     /translation="MAQRARISLSGTDPEKVDSVCSQIKQISTRTGVAIRGPIPLPTK
                     RLQVPCRKSPDGEGSETWDRWEMRIHKRLIDLDADERALRQLMRIQVPDGVNIEIVLR
                     S"
     gene            8170..9039
                     /locus_tag="PLJ11_00150"
     CDS             8170..9039
                     /locus_tag="PLJ11_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOO03118.1"
                     /translation="MAEPTIFGLSIPDLVGFILLTILTVLLAKGTSMLMRRLLDYRIP
                     RTYSKAIAKLAQYIVLAAGLYIGFWEVLGLDIAALLASLGVLGIGVALASQQVLSNAV
                     SGVLMSVVRPVRVDEWIEVAGVPATGICRVKDINLMNTILKDDDGRILYVPNSFMINN
                     KVVNYSRGGVVALRIPVWLRSLKDFDHIKDIVLDVADKDPLILPNVGEEERKNILKIF
                     ELANVRKYLGKKVDMAYFSPSIVIKDIQREKVKVDIKIWVTDVSKRDEIITGYLDALR
                     QRFALEGIEFGDD"
     gene            9081..10727
                     /gene="ilvD"
                     /locus_tag="PLJ11_00155"
     CDS             9081..10727
                     /gene="ilvD"
                     /locus_tag="PLJ11_00155"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOO03119.1"
                     /translation="MRSDAIKKGVDRAPQRSLLRATGLTDSDMEKPLIGIANSWNDVV
                     PGHIHLNELAEEVRMGVIEAGGVPLTFGVPGVCDGVAMGHAGMRYSLVSRDVISDCVE
                     IMLQAHCMDGWVGVTNCDKITPGMLMAAGRLDLPTIILTGGPMEPGRRDDIGLDLQSV
                     FEALGANKAGTMTEEEVLEIERCACPGEGACSGLFTANTMACLTEALGLSLTGCGSML
                     ATDQRKKEMARATGRRIVELVREGLTVRKVVTKGSFTNAIILDMAIGGSTNTALHLPA
                     IAADFGIEIDLRTFDEISRRVPHLTSIKPSGPYYMKDFDAAGGVSAMLNRLMDLLSDE
                     RTVNGMSIKEVARRATVRNEEVIRPRDRPFHAEGGIAVLYGNLAPKGSVVKQAAVSSG
                     MMRFVGTAKVFDSEQAAIEAISTRQISAGDVVVIRYEGPKGGPGMPEMLSPTSLIAGM
                     GLADSVALITDGRFSGATRGPCIGHVCPEAFDKGPIAAVRNGDRISIDIPGRKLELLV
                     PQKEIEDRLAKVQVVDRHPTGVLAKYRKLVGDASTGAICR"
     gene            10771..10844
                     /locus_tag="PLJ11_00160"
     tRNA            10771..10844
                     /locus_tag="PLJ11_00160"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10805..10807,aa:Ala,seq:tgc)
     gene            complement(11157..12254)
                     /locus_tag="PLJ11_00165"
     CDS             complement(11157..12254)
                     /locus_tag="PLJ11_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO03120.1"
                     /translation="MALSVGIFGLLILAIIAAIVILVNGIKIVRPYEQAVYIRLGKFI
                     RVLNPGFNYVAPLINETVKLDLRTQVLDVPRQEVITKDNSPVNVDAIIYVKVIDPKKA
                     FFEVTDYRAATVYLAQTTLRSIIGDMELDEILSNREKINLKLRDILDEATDQWGVKVD
                     AVEIREIDPAPKVKQAMEEQTSAERLRRAAILRADGEKTAAILNAEGEKRSRILQAEG
                     LRQAKILEAEGERLAIILQSQGESQKLRILSVGASTLDTKALTVLSLDAVKSLGEGQA
                     TKIVLPFEVSRLVEGISDYIGAGKQVPEREISNPEEIEKVVGKADAILGPIPTMEELR
                     ADMKKLEEDMAKDKRIAEAITSQGKLAQQAP"
     gene            complement(12276..12722)
                     /locus_tag="PLJ11_00170"
     CDS             complement(12276..12722)
                     /locus_tag="PLJ11_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOO03121.1"
                     /translation="MSLATDLSLAFIVIGVLMLLLEMSQPGSFLIVPATVLLVLGGIG
                     LIFPDILLTIWSPIIAVIILIPTTLLTIKFYQKLAPPTPPETTVATSLIGKEGVVEVE
                     VVPGSLKGKVRIANVIWSATSTAPIPVGKRVRVIQSEGVHVTVEEI"
     gene            12910..13575
                     /locus_tag="PLJ11_00175"
     CDS             12910..13575
                     /locus_tag="PLJ11_00175"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOO03122.1"
                     /translation="MCENTEDDMVGLPLIGDRFPEMVVKTTHGTIELPKDYAGKWMVL
                     FSHPADFTPVCTTEFIGFAKKNAEFEQMGAKLIGLSIDQVHSHIKWVEWIEEHMDVKI
                     PFPIIADTGNVAIQLGMIHPNKGSNTVRAVFIVDPVGVIRLIIYYPQEIGRSIDEVLR
                     AVKALQFADKNHVSTPENWPNNDFLKDRVIVPPPSDYKTAMERKKSMGKDCYDWWFCT
                     KKV"
     gene            complement(13781..15052)
                     /locus_tag="PLJ11_00180"
     CDS             complement(13781..15052)
                     /locus_tag="PLJ11_00180"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOO03123.1"
                     /translation="MQTVTLLAFLVFLFTYALISVRRVRSVSIERPAAALFGALLMVL
                     LGVVTADQVVEAIDLDIIGLLLGMMIIVSCLEITGLFDRVASTMVDRCGDRFTLLWVS
                     MGITALLSALILNDTVVLMFTPIIVKVCRSIDANPIPYLVGEALAANIGSVATGVGNP
                     QNAYILIQSGIPFTEFAIALTPLAVMSLLVAVAIVALVFRKDLFDEGLRPHPIDRSLL
                     AAASPKVRFEIPFFLVLGVLIAVFIGFLASSWLGMPVSLIAFLGGCFLLLALPLVKKD
                     TGTGPILRGVDWTLILFFVGLFILLKGVETSGLMALMLGSFEDMGAGVAGVAGLTVVS
                     SVLSNLISNVPAVMLLSPMIPVGDDTLWLSLATSSTLAGNATILGAAANVIVVEKGLS
                     MGAEVRFLDFLKAGLPVTLVTLLMSVLLLGL"
     gene            complement(15093..15671)
                     /locus_tag="PLJ11_00185"
     CDS             complement(15093..15671)
                     /locus_tag="PLJ11_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOO03124.1"
                     /translation="MRVRKAAFLFGTALSPALISDRAIPCWTAKGAGTSNIGEDGIHR
                     GMGQDDLDEVILRLLQANGKMSIEEIAERVGRSPSTVRDRVRRMEEERVILGYSTIVD
                     EDRLNIEAEALVLADIEPEAEGRAMGALMALEAVSEVMKVTGEPRIMFRIRASDRHEL
                     LRYLDRYIRPLGFRRMDVRLVLDHTVRYPGTV"
     gene            15996..16982
                     /locus_tag="PLJ11_00190"
     CDS             15996..16982
                     /locus_tag="PLJ11_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOO03125.1"
                     /translation="MQNLRKRVPTILTSDEVLDKAFRKVSKIDKQGPDKVELVKRKSM
                     ARIASATDVISSTFERYVKAFPSINDRGDFLAEMIEVTVGVDQLKKALSNLDWAASKV
                     KELQRQYTSKVMASSSVQGADSARKAFYGRASSVVNKVAPDLGFLAYARNEFRKVPDI
                     DEGLLTFVIAGFPNVGKSQFLSLVSSAEPEVAAYPFTTKGIGVGMHEAGYRRYQVIDT
                     PGLLDRELEDRNRIEMQAVTALKHLANAILFILDPSETCGYPLDRQLRLLESIKGLFP
                     DVPILVVENKVDLLDSGSDRMKMSAATGQGVPEVMAALRDSVRHLEKAEPLF"
     gene            17029..>18829
                     /locus_tag="PLJ11_00195"
     CDS             17029..>18829
                     /locus_tag="PLJ11_00195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177373.1"
                     /note="unique DNA polymerase that is similar to eukaryotic
                     polymerases; forms a heterodimer of the small and large
                     subunits; possesses polymerization and 3' to 5'
                     exonuclease activities; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HOO03126.1"
                     /translation="MQELACSGPMRRYFSTLFQENERCYALAAEARGRGLDPSTEVEI
                     PQAEDLAARVEKQLSDWHVEGVAERIRGLSKRIESREEVSLYIAKELAKEAVAAHEGR
                     EMAIERAVRVGLSVLTEGILVAPIVGIAGVKINPNPDGTEYLSVFFNGPIRAAGGTAQ
                     AMSVLIADVVRREFGIGAYKPTTGEVERLKEEIPLYKQCQHLQYTPSNEEIEIIYRNC
                     PVCVDGEKTEDIEISGFRNLPRIKTNAVRGGVCLVIAEGLCLKAPKLEKHVRKLGLEG
                     WDFISEYLRRKRGTGKEENTKVAPDYKYLKDIVAGRPVIGHPSRPGGLRLRYGRGRTT
                     GLAAIALNPATMYALDSFLAIGTQIKIERPGKAGAVTPCDQLEGPILLLDSGDLVQAN
                     TVAEFMPYEGRVKEIVDVGQILLPFGEFVENNHVLMPADYDLEWHRAELRSANGGELP
                     EGWEDPSFDSALAMSARYGVPLHPKHNLFWYDHSIQELQALRTHILREGQVVAGSLLV
                     PLEPVSKRTLETLGALHRVVDGKVIIDQYTDPLLAGLGLRGKDGVEDLTPLEGASVME
                     AVSRCTGVKVNPRSVTRIGCRMARPEKAKDRSAK"
BASE COUNT         4103 a         5594 c         5758 g         3374 t
ORIGIN      
        1 aatgatacgg cgaccaccgt gtagaagagc gcatgctccc tggaaagggg ctcgaacccc
       61 tctaggaacc cctggtgcct ctccagcgcc cttagggcgt ccagcagggc ggggtgcgag
      121 cggcagcgcc tttccacaag ctcccagagg tccccctcga ggatggccct ccttacccgg
      181 tccagctctg tcttcgagat ccaaaggttg tgcctggcta tgagcgctgt cctctgggcc
      241 gggggcatcc ctcttatctc ctccaggcca tggccgaagc aagccgggca ttggcattcc
      301 agggccttca tgtcctgcag gcggaacgtc ccctcgagga acatgaagcg gtcatcgcgg
      361 gcgaacttgg cgtaggaggc cgaatcgaac atgtcgcacc ccagcagcgc cgcgagggcg
      421 aagagcatcg gatgccccgc cccgaacagg tgcactgggc ggtccggccg aagccccttc
      481 ttggccgcga cgatcacgtc cacaagctcg gcgaagcggt agttctccat gagcggtacc
      541 acgccgccga tggggttcac gtcgacctcc atctcggaga ggcggcgggc acacatctcc
      601 ctcaggtcag ggtatatgga cccctgcacg acgcctgcga ggagcatctc ccccttcaag
      661 cccgcggcct ccatggtcct ttccaatgtg acctcgaggg aacctgcggt gcgctccttg
      721 ctccaatccg gttccgcgaa gatatccagg accgtcccga tatcggaccc tatgtcccgt
      781 tggaactcca cgatgtcccg gttgatcaag tccacatcgc catacatatg ggattggaag
      841 gtcccggagt ccgtcatgat gatccccggg tagtcgagca tggcgtgcac ccccccttcg
      901 agggcctcct ccctcaatcg ctgatcgttg cgaatgatgt acgaattggt gatcaaggcc
      961 cggaagccga agacgtcata taactccctc ggggagatcg tgaccaaccg gggattgatg
     1021 accggcagga ggcagggggt ctccagctct ccctggcggg tatgcagcct gcagatcctg
     1081 gccaagccat cgcgcctaac tagctccaac atgagcgccc catcccttcg agttatatta
     1141 aagatgacag cacgctcccg caggaaaggg atatattgac ggcaggaaat ggcctacctc
     1201 gatgcaatgg aatcccttgt gatgtgctgt ccccgctgcg atatcctcat caccgtgatg
     1261 gtccaggact gcctcgaggg cagggagttt ccctgcccca tctgcggtga ggtgatagcc
     1321 ttcaagttca cccccgagga gatggagcaa aaactagaga tggccaagaa gaaccaggaa
     1381 cgcgccctgc gccgacagtt ccccctgacc ttcggggatg tgtgaaccct tatggagttc
     1441 gagctcaaga tggtcatcgt ggtccgcaag gacctcaaac tgtcccccgg caagatggcc
     1501 gctcaggtgg cccacgcggc ggtgaactgc gccctggcca gcaagaagcg gaggccgatc
     1561 aacttcgacc gttggtacag tgagggccaa aagaaggtcg tgctcaaggc ccaggacctg
     1621 aatgagctca tccagatcaa gcaggccgcg gaggacgccg gcctggtgac ctccctgatc
     1681 accgacgccg gcttgacaga gctgccccct aacacgacca cctgcctcgg catcggcccg
     1741 gcccccaacg ccgagatgga ccggatcacc gggcacctgg ggctgatgta ggtgaaggac
     1801 caggcccgcg tcctgggcat agatgacggc cccttcatca aggggcaggc gcgcgccccc
     1861 ctggcagggg tattggtctg ccctcccggc tatgttgaag gcatactagt ctcttcctgt
     1921 caggtggacg gcgaggacgc caacgaggcg atcgtcgaga tggtcaggga ctcacgcttc
     1981 ttcgaacagg tccgcatggt catggtggat ggggccgccc ttgggggctt caacgtcgtg
     2041 gacatccaag ggctcagcgc cgagctcggg attccggtcc tcaccgtctc ccgtgatgaa
     2101 cccaacatgc gctccatctc ctccgccctc cgtgcgcact tcagcgactg ggagcgaagg
     2161 ctggaggtca tctctcggag tgccatcagg acggtggagg tgcccgatgg ccgagtgttc
     2221 gtggcctcgg caggcatcgg agaaaaggag gcagatgcca tggtccgccg gtgcacggtg
     2281 cgcggctgcc tcccggaacc gataaggctc gcgcacctcg tggccaccgc cctggtgcgc
     2341 ggagaatcca aaggaagggc gtgaaagaaa ggattatctt ctgaggttcc tcatccttat
     2401 tacatggacg acctaatatc gaaggtggcc gaggagcgcc taagggcgat ccagagggag
     2461 cttccccctg ccgaggtcgt cacgcatttc cacgaggacg agcgagggcg agggctgatc
     2521 gacattgtct accagggggc cctggtcggt caggagatca tagagaccca cgattcgtgg
     2581 aaggaggaag ggcgactggc ggcctaccag gccgtattga ggaagaaggt taggcttgtg
     2641 gtgatggccc ctagggggga ggccatgagg gtccgctata tgatgctcga gctaaataac
     2701 tgctggctct tctattatat ggtctatagc tatgaccctg acggccatct aatgagggtc
     2761 ctgcgacctg ctgtccccgg ccgtagggag cccgcggtcc catacatcca atgagccccg
     2821 gcaaccgtca aagcacaagc ccatgatatc ggccaggatt gcttgttggc cacgttccac
     2881 agtcccatat tcttatatag gaaatcggta ataggtgcgg tgcccctgaa tttgaggtga
     2941 cacatttggc aaaaggatta ttcactgcca gaaagctgag gaacgatcgg cagaaggccc
     3001 gatggagcga caggtcctac aagagaaggc tgttgaagct caaggagagg tccgatccgc
     3061 tcgagggcgc ggcccaggcc aaaggcatcg tgctggagaa ggtcggcatc gaggccaagc
     3121 agcccaactc ggccattcga aagtgcgtca aggtccagct gatcaagaac ggccgtcaga
     3181 tcaccgcgtt cgcggtgggc gacggcgcca tcaatttcat cgatgagcac gacgaggtcc
     3241 tggtggaagg catcggaggc aggctaggcc gttcctacgg tgacattccc ggggtaaggt
     3301 acaaggtaat caaggtgaac aacgtctcct tgaacgaatt ggtcagggga aggaaggaga
     3361 agccggtcag gtgatcccat tggaagaggg cagtttcaag ttcgacaata tcctgctgtt
     3421 cggcaagtat tcgagcggtg acgtcgtcgt gaatgacggg ggattggcca agtacatcaa
     3481 cttgaccccc accgccgtgc ctcacaccac cgcccgccat gccaacaagt ggttcgggaa
     3541 gtccaaggtc aacatcgtgg agcgcctgat caacaacatg atgaggaccg aggtcttcac
     3601 cggaaagaag ctgaaggcct acaatgtgac caagaaggcc ttcgagatca tcgagcagcg
     3661 caccaagcgg aacccgatcc aggtcctggt cgaggccctg gagaacgccg ccccgcgcga
     3721 ggagatcacc cgcctgcagt tcggagggat atccgtcccg aaggcggtgg acatagctcc
     3781 ctctcgccgg ttggacatcg cgctccgcaa cataacgaag ggaacggtca acgcctcatt
     3841 caagaacacc aagcccatcg agcagtgcct ggctgaggag ctgatcctgg cctccaaggg
     3901 cgacatgaac agcttctccg tggccaagaa ggaagagatc gaaagggtcg cctcctccgc
     3961 gcgttaacgc aggtggggaa aatgggcaga aaagaggaca atatccgaag ggctcaggag
     4021 atcatgctga agcccgagtt catcaggaac atcggcaccg ccgcgcacat cgatcacggg
     4081 aagaccaccc tgagcgacaa cctcatcgcc ggagcgggca tgatgtcgga gaacctcgca
     4141 ggcaagcaat tgatgctgga cttcgacgag caggagcagg cgcggggcat caccatcaac
     4201 gcggccaacg cctcgatggt ccatcattgg aagggcaatg actatctcat caatctcata
     4261 gacacccccg gccatgtaga cttcggcggg gacgttacca gggccatgag ggccctggac
     4321 ggatgcatca tcctggtctg cgccgtggag ggcatcatgc cccagaccga gaccgtcatc
     4381 cgccaggcgc tgaaggagag ggtcagaccg gtcctcttcg tcaacaaggt cgaccgccta
     4441 atcaacgagc tgaaggtgac cgagcaggaa atgcagcagc ggttcgttca gatcatctcc
     4501 gaggtcaaca agaggatcgc ggcgaacctg cccgaggacc tcaggaagaa atggcaggtc
     4561 aaggtggagg acggctcggt agccttcggc tccgcattca acaactgggc gatatccgcc
     4621 ccgtacatga agaagagtgg catctccttc aaggacgtgt acaactactg caagaacgag
     4681 gaccagaaga ccctggccaa gaaatcgccg gtgcacgagg tcctgctgga gatggtcatc
     4741 acccatgtcc ccagtccgct ggaggcccag aaggtccgta tccctaccat ctggaagggc
     4801 gacctgaact ccgaggtagg cagggccatg atgacctgcg accggaacgg ccccacgacg
     4861 ttcatgtgca ctaagatcat catcgacccc cacgcggggg aggtggccgt aggccgcctg
     4921 ttctctggca aggttgtccg aggccaggag ctttacgttc ttgggatgcc caaccccaac
     4981 cgggtgcaga ccgtagcgtt gagcgtcggc gccgaccgca tcgccgtgga cgaggtggag
     5041 gcaggcaaca tcgtcgcact gaccggattg aaggacgcca tcgcaggggc gacctgctcc
     5101 tccgagaagg acatggagcc cttcgagaag atcaccgact acaccgagcc cgtagtaacg
     5161 gtggccatcg aggccaagca catgaaggac ctgcccaagc tggtcgatgt gctgaggacc
     5221 attgggaagg ccgatccgtc catccaggtg cagatcaacc aggagacggg ggagaacctc
     5281 ctgtccggga tgggagaact ccatctggag atcacccaat atcggatagt gaatgaccac
     5341 aaggtggaaa taaaggcctc ggccccgatc gtcgtctacc gggagtccat caagggaagg
     5401 ggcggaccgt tcgaggggaa gtcccccaac aagcacaaca agttctacat catggtggaa
     5461 ccgctcgagg aggcggtgat cgaggcgatc aagaatgggg agatcaagac cgaggggaaa
     5521 ataaaggacc ccaaggcctt ggccaagcag ctgtccgacc tgggaatgga caaggaacag
     5581 gccaagggcg tggtcgcctt caagaacaac aacgtgttca tagacgtcac caagggcatc
     5641 cagaacctgc acgagaccat cgagctctgc aagcaggcct tcgaggaggc catggagatg
     5701 gggccgctgg caggtgagcg cgtttccggc gttaaggtcc tgctcgtcga tgccaagctg
     5761 cacgaggact ccatccacag gggcccaggc caggtcattc ccgccacccg ctccggcatc
     5821 tatggcgcca tgtgcatggc cggaagggtg ctcctggagc ccatacagaa ggtctatatc
     5881 aactgccccc aggatatgat gggcgacgtc accagggaac tgcaaggccg ccgggcaacg
     5941 atcgaggaca tccgtcagga acatgacgcg acggtcgtcg tggccacggc cccggtggcc
     6001 gagatgttcg ggttcgcgag cgcgatccgg ggtgccactc agggaagggc gctatggtcg
     6061 accgagaatt ccggcttcgt gatattgcca agggagctgc aggacaaggt cgtcggagag
     6121 ataagaaaga gaaagggact gaacccccag ccgtacgatg cggcctatta ctccggctga
     6181 acatgaaaaa cggttatata gaatgaataa tatcgagagg caaccaactc atagtatata
     6241 aggaaaaact ggagggaaaa caaatggctg acaaacctca catgaatctg gtctttatag
     6301 gtcacgtcga ccacggcaag tccaccaccg tcggcaggat gctgctggac accggtgcga
     6361 tcgaccccta cgtgatcgag aagtacagaa agcaggccga agagaaggga aaggcgacct
     6421 ttgagttcgc ctgggtcatg gacagcttga aggaggagag ggagcgcggc gtgaccatcg
     6481 acgtgtccca caagcggttc gacaccgaca agtactactt caccgtgatc gacgcccctg
     6541 gccacaggga cttcgtcaag aacatgatca ccggcaccag ccaggccgat gccgccgtcg
     6601 tggtcgtctc agccatcgaa ggcccccagg cccagaccag ggagcacata ttcctggcca
     6661 agacgctggg cgtcaagcag atcatcatcg ccgtcaacaa gatggatgcc gtgaagccgc
     6721 cctatgacga ggccgcctac aagaagaccg tcaacgaggt cagctccttg ctgaagacgg
     6781 tcggcttcaa ggtcgaagag atcccgttca tcccgatcag cgcgttcaag ggcgacaatt
     6841 gcggaaagag gtccgagaac atgccctggt acaagggcga cacactgctc gaggccttga
     6901 acaagctcaa ggtgccccag tcgaccgagg ggctcccgct ccgcctgccc attcaggacg
     6961 tatatacgat cacgggcatc gggaccgttc ccgtcggccg ggtcgagacc ggggtcctga
     7021 agccggacat gaaggtcata ttcatgccct cgaacaaggt cggggaggtc aagagcatcg
     7081 agatgcacca cgagaccctg cccgttgcga aaccgggcga caacgtcggc ttcaacgtca
     7141 ggggcatcgc caagaacgac atcaagaggg gggacgtcgc cggaccggtg gacaacccgc
     7201 ccaccgttgc caagacgttc accgcccaga tcgtggtgct gaaccaccct accgccatcc
     7261 ctgtcgggta caccccagtg ttccactgcc acaccgcaca ggtggcatgc acgttcatcg
     7321 agctgcagaa gaagctggac ccgaggaccg ggcaggtaaa ggaggagaac ccgaccttcc
     7381 tgaagaccgg ggatgtcgcg gtcgtcaagg tcatgcccac caagcccctg gtgatcgaaa
     7441 gggccaagga cttcccgcag ctgggcaggt tcgccatccg cgacatgggc cagacggttg
     7501 cggccggcat gtgcatcgat gtcgaaaaga aggagatgta aaccctttcc gccctttcat
     7561 ttcggggatt gacacatggc gcagagagca aggatatcat tgagcggcac agacccggag
     7621 aaggtggaca gcgtttgcag tcagatcaag cagatctcca cacgcactgg cgtggccatc
     7681 cgcggcccca ttccgctgcc gaccaagagg ctgcaggtgc cctgccgcaa gagccctgat
     7741 ggagagggct ccgagacctg ggaccggtgg gagatgcgga tccataaacg cctcatcgac
     7801 ctggatgcgg acgagagggc cttaaggcag ctgatgagga tccaggtgcc ggacggggtc
     7861 aatatagaga tcgtgctcag gtcctaatgg caaatccctt aaccaatatc ttcccgtttt
     7921 gtttttcttg ttaatcggcg cgctaaccgc atggcatatg ggcgttcctg aacatgcttg
     7981 atttcttgca cgccctctga tccccaatgg atggcccatc gaggacaggc agaggctcag
     8041 gaatttcctc ggatcgcgcc aattggccag gtggcgatga cgtctttgct cgatgccatt
     8101 ggcgatcggt gggggaaggc cgggcaccag gcgcaaactt attaaacggc tgcacatgta
     8161 gaagggcaga tggctgaacc gaccattttc gggctctcga tcccagacct agtgggcttc
     8221 atcctcttga ccatcctcac ggtattgctg gccaagggca ccagcatgct catgaggagg
     8281 ttgttggact atcgcatccc acggacctat tccaaggcca tcgccaagct cgcccagtac
     8341 attgtcctgg cggcggggct ctatatcggc ttctgggagg tgttagggct ggacatcgcg
     8401 gcgctcttgg cctcgctggg ggtactcggt ataggggtcg ccctcgcctc ccaacaagta
     8461 ctgtcgaacg ccgtctccgg ggtcctcatg tccgtcgtca ggccggtaag ggtcgatgag
     8521 tggatagagg tggccggggt accggcgacc ggcatctgca gggtaaagga catcaacctc
     8581 atgaacacca ttctaaagga cgatgatggc cgcatcctct acgtgcccaa ctccttcatg
     8641 atcaataaca aggtcgtcaa ctattcccga ggcggggtgg ttgccctccg catccccgtc
     8701 tggttgcgct cgctcaagga cttcgaccac atcaaggaca tcgtgctgga cgtggcggac
     8761 aaggacccgc tcattcttcc caatgtaggg gaggaagagc ggaagaacat cctaaagatc
     8821 ttcgaactgg ccaatgtacg gaaatacctt ggaaagaagg tggacatggc ctatttctcc
     8881 cccagtatcg tcatcaagga catccagagg gagaaggtga aggtggacat caagatctgg
     8941 gtgacggacg tgtcaaagag ggacgagatc atcactggtt atcttgacgc gctcaggcaa
     9001 cgatttgccc tggaagggat cgagttcggg gacgattgag acaagatatt aatctaacag
     9061 cgcggacata ccggtccccg atgcgaagcg atgcgatcaa gaagggagtg gaccgggccc
     9121 cgcagcgcag ccttctgcgc gccaccggtc tgaccgactc ggacatggag aagcccctaa
     9181 tagggatcgc caattcctgg aacgacgtgg tcccagggca catccatctc aatgagcttg
     9241 cggaggaggt gcgcatgggc gtgatcgagg caggaggcgt tcccctgacc ttcggcgtcc
     9301 cgggggtctg cgacggagtg gcgatgggcc acgcaggcat gcgctattcc ctcgtctcca
     9361 gggatgtcat ctccgactgc gtggagatca tgctccaagc gcattgcatg gacgggtggg
     9421 tcggggtgac caattgcgac aagatcaccc ccggaatgct catggccgcc gggaggctcg
     9481 atcttccgac catcatcctg acgggcgggc caatggaacc gggcaggagg gacgacatcg
     9541 gtctggacct gcagagcgtg ttcgaagccc tgggcgctaa caaggcaggg acgatgaccg
     9601 aggaggaggt gctagagatc gagaggtgcg cctgccccgg agagggcgcc tgctctgggc
     9661 tgttcaccgc caacaccatg gcatgcctta ccgaagcgct cgggctcagc ctcacagggt
     9721 gcgggtcgat gctagccacc gaccagcgca agaaggagat ggcccgggcg acaggaaggc
     9781 gcatcgtcga actggtgagg gaaggcctga cagtccgtaa ggtcgtcacc aagggctcgt
     9841 tcaccaacgc catcatcctt gacatggcca tagggggctc caccaatacc gccctgcact
     9901 tgccggcgat agcggccgac ttcgggatcg agatcgacct ccgcaccttc gatgagatct
     9961 ccagacgggt ccctcacctg accagcatca agccgagcgg gccctactac atgaaggact
    10021 ttgatgcggc gggaggtgtg tcggccatgc tcaatcggct gatggatctc ctttcggacg
    10081 agaggaccgt aaacggaatg agcataaagg aggtcgcgcg ccgggcaacg gtgcgcaacg
    10141 aggaggtcat ccgccctagg gaccgaccgt tccatgccga ggggggcatc gctgtcctct
    10201 atggcaattt ggctcccaaa gggtccgtgg tcaagcaggc cgcggtctcc tcgggaatga
    10261 tgaggttcgt ggggacggcc aaggtcttcg attccgaaca ggcggccatc gaagccatct
    10321 caacccgtca gatcagcgca ggggacgtcg tggtgatccg gtacgaaggc ccgaagggag
    10381 ggccggggat gcccgagatg ctatccccga caagccttat tgccgggatg gggctggccg
    10441 acagcgttgc gttgatcacc gatggcaggt tctccggcgc caccaggggg ccatgtatcg
    10501 ggcatgtatg ccccgaggcc ttcgacaagg gccccatcgc cgctgtgagg aacggcgaca
    10561 ggatctccat agacatcccc gggcgcaagc tcgagctgct ggtcccccag aaggagatag
    10621 aggaccgcct ggccaaggtc caggtcgtgg accgccatcc aacaggggtc ctcgccaagt
    10681 atcgcaagtt ggtcggggac gcctccacgg gggccatatg ccgctaggcg cgaaacctat
    10741 ttattcgaac tcattttatg cggccccact gggcgggtag atcagctcgg aagatcgcct
    10801 gctttgcaag caggaagccc cgggttcaaa tcccggcccg tccatccttc ccccgctccc
    10861 ttccttccgg gcgatgggcc caaatgatgg ggtaatcgaa gggcattcct tggaaaagag
    10921 gttgatggct cgaaggtcca tagggctctc gaggccatca aaggacgtgc acgggcatcc
    10981 tgaaccccgc gtcaaagacc caggtatcca agggactgat cgatgggcaa gaacggttag
    11041 tagggaccgg ggggaattac ggccccaggt caaacccttg ctcggtcccc ccactccggg
    11101 cgcggtgatg tccggaaagg gcggcccggc aggcgattga aaaaaaagga aggtgtttag
    11161 ggcgcctgtt gggcgagctt gccctgcgac gtgatggcct cagcgatcct cttgtccttg
    11221 gccatgtcct cctcgagctt cttcatgtcc gcacgcagct cttccatggt ggggatcggc
    11281 ccgaggatgg catccgcctt gccgaccacc ttttcgatct cctccggatt ggatatctcc
    11341 ctttccggca cctgcttgcc cgccccgata taatcggaga tgccctcgac cagcctggag
    11401 acctcgaacg ggagcacgat cttggtggcc tgcccctccc ccaggctctt caccgcatcc
    11461 aatgagagga cggtgagcgc cttggtgtcc agcgtgctgg cgcccacgct caggatccta
    11521 agcttctggg actcgccctg gctctgcagg atgatcgcca gcctttcgcc ctctgcctcc
    11581 aatatcttgg cctgcctgag cccttccgct tggaggatgc gggacctctt ctcgccctcg
    11641 gcgttcagga tggcggcggt cttttccccg tcagctctga ggatcgccgc ccgcctcagc
    11701 cgctccgcag aggtctgctc ctccatggcc tgcttgacct tcggggcagg gtctatctcc
    11761 ctgatctcca ccgcgtccac cttgacgccc cactggtccg ttgcctcgtc gaggatgtcc
    11821 ctcagcttca ggtttatctt ctcacggttg gacaggatct catcgagctc catgtccccg
    11881 atgatggacc tcagggtggt ctgcgccaga tagaccgtcg cagccctgta gtcggtgacc
    11941 tcaaaaaagg ccttcttggg gtcgatgacc ttcacataga tgatagcgtc cacgttcacc
    12001 ggggaattgt ccttggtgat gacctcctgc ctcggaacgt ccaacacctg cgtcctcagg
    12061 tccagcttca ccgtctcatt gatcagcggg gcgacgtagt tgaaccccgg gttcaatact
    12121 ctaatgaact tgcccaggcg gatatagacc gcctgctcat aaggtctgac gatcttaatg
    12181 ccgttcacca atatgacgat ggccgcgatg atcgccagta tcaagagccc aaatattcca
    12241 acgcttaatg ccatgatctc tctccttttc gctcctcaga tctcttccac ggtcacatgc
    12301 acgccctcgc tctggatcac cctcaccctt ttccccactg ggatgggggc ggttgaggtg
    12361 gcgctccaaa ttacattagc tatccgcacc tttcccttaa gggagccggg tacgacctcg
    12421 acctcgacca ctccttcctt gcctatcagg gaggtggcca ccgttgtctc cggcggggta
    12481 ggcggggcca atttctgata gaacttgatg gtgagcaggg tcgtgggaat gaggatgatg
    12541 accgcgatta tcggggacca tatggtcaat agtatgtcgg ggaagatgag ccctatgcct
    12601 cccaggacca ggagcaccgt ggccggtact atgaggaacg agccaggttg gctcatctcc
    12661 aataggagca tcaggacccc gatcacgatg aacgccaatg ataggtcggt agccagggac
    12721 atcgttcatc cgatagggtt ctctctattt atctttacca tcgatattcg tacggcaggt
    12781 tcgggttacg aagggacgca cgatgccgaa agggaccgga cgaggccagc taaaggtgtc
    12841 ctttcaccat ttctccgatc gaaataccat taaataatat gcccttccgt actagcatca
    12901 gtgatatcaa tgtgcgagaa cacggaagat gatatggttg gcctgccctt gatcggggac
    12961 agattcccag aaatggtggt caagaccacc catggtacga tcgagctgcc taaggactat
    13021 gcgggcaagt ggatggtgct gttcagccat ccagcggact tcaccccagt gtgcacgacc
    13081 gagttcatcg ggttcgcgaa gaagaacgct gagttcgaac agatgggcgc caagctcatc
    13141 ggtctttcca tcgaccaggt tcactcccat ataaagtggg tggagtggat cgaggagcac
    13201 atggacgtga agatcccctt cccgataatc gccgacaccg ggaacgtggc catccagttg
    13261 ggcatgatcc acccgaacaa ggggagcaat accgtccggg ccgtcttcat cgtggacccc
    13321 gtgggggtca tccgcttgat catctactat cctcaggaaa taggcaggag catcgatgag
    13381 gtgctcaggg cggtcaaggc gctgcagttc gctgacaaga accatgtatc aaccccggag
    13441 aactggccga acaacgattt cctcaaggac agggtcatcg ttccgccccc gtccgactac
    13501 aagaccgcca tggagaggaa gaagagcatg gggaaggact gttacgactg gtggttctgc
    13561 accaagaagg tctgacctca gcgcaaatgt ggcctgttcg gccacctttt tttttccttt
    13621 cacccttccc ctcaggcaat ccatcgaaga gaagggcgcc atgaccagga cggctgcccg
    13681 aggccatcct cccatggtcg ctatcccacc ggccgatgcc ccgccccctc acctaacggc
    13741 ccttgacgcg cgggcgaacc ctcggcccgc cttccgacca tcaaagaccc aggagaagca
    13801 ccgacatcag taaggtcacc agggtcacag gcaaccccgc cttgaggaag tccaagaacc
    13861 ggacctccgc ccccatgctc agccccttct cgaccacgat gacgttggct gccgctccca
    13921 ggatcgtggc gttccccgcc aaggtggagg aagtggccag gctcaaccac agggtgtcgt
    13981 cgccgaccgg aatcataggg gacaggagca tcacggcggg cacattgctt atcagattgg
    14041 agagcaccga agagaccacg gtgaggcccg ccacgccggc cacgcccgcg cccatgtcct
    14101 cgaacgaccc gagcatcaaa gccatcaggc cggaggtctc cactcccttg aggagaatga
    14161 aaagccctac gaagaacagg atcagggtcc agtccacgcc acggaggatc ggccccgtcc
    14221 cggtatcctt cttcaccaac gggagggcca acagaaggaa gcagccccca aggaaggcga
    14281 tgagggaaac gggcatgcca agccaggagg aggccagaaa accgatgaac acggctatga
    14341 gcacacctag cacaaggaag aaggggattt cgaaccgcac cttgggcgag gcggccgcca
    14401 ggagcgagcg gtcgattgga tggggccgca gtccctcatc gaagaggtcc ttgcggaaga
    14461 ccagcgccac gatggccacc gccacgagca gggacattac ggcaaggggg gtcagggcaa
    14521 tggcgaactc ggtgaagggt atccccgact ggatcaaaat gtaagcgttc tgcgggttgc
    14581 ccaccccggt ggccacggaa cctatgttcg ccgccaaggc ctccccgacc aggtagggga
    14641 tcggattggc gtcgatggaa cggcacacct tcacgatgat cggggtgaac atcagcacca
    14701 ccgtgtcgtt caggatgagc gcggagagca gggctgtgat ccccatgctc acccatagga
    14761 gggtgaagcg gtccccgcat cggtccacca tcgtgctggc cacccggtcg aagagtccag
    14821 ttatctcaag gcaggagacg atgatcatca tccctaggag cagcccgatg atgtccaggt
    14881 cgatggcctc cacgacctgg tccgccgtga ccactcccag cagcaccatg agcagcgctc
    14941 cgaacagcgc ggcggccggc cgttcgatgc tcacgctgcg gacccttctc accgatatga
    15001 gggcatatgt gaagagaaag accaggaagg ccaacaaggt gacggtctgc atccggggaa
    15061 tatagatgat gagaataatt aagccaatcc tttcaaaccg tccccgggta tctgacagtg
    15121 tgatccagga ccaatcgcac atccatcctg cggaagccga gcggcctgat gtaccggtcc
    15181 aggtacctga ggagctcatg acggtcagag gctcggatcc ggaacatgat acgcggctcc
    15241 ccggtcacct tcatgacctc gctcacggcc tccaaggcca tcaaggcgcc catggccctg
    15301 ccctccgcct ccggctcgat gtccgccagc accaacgcct ccgcctctat gttcagcctg
    15361 tcctcgtcca ctatcgtgga atagcccagg atcacccgct cctcctccat gcgacggacg
    15421 cggtcccgga cggtcgaggg ggagcggccc actctctcag cgatctcctc gatgctcatc
    15481 ttcccattgg cctgcagcaa acgaaggatc acctcgtcaa ggtcgtcctg tcccatgcct
    15541 ctgtgtatcc catcctcgcc tatattagat gttcccgcgc ccttggccgt ccagcaagga
    15601 atggcccggt cggagattag cgccggggaa agggctgtgc cgaagaggaa cgcggccttt
    15661 cgcactctca tttggaatag acgcccctcg cccatcctca agcccgctcc gtcccttcct
    15721 ctcttgaacg aacgttcttc atatccaagg ggaaaaggaa ggaacgatag gtcagtcctt
    15781 ccatggcgaa tggctctccc atgcagttcc aattcgtcgt ctcctcccct ttgggctgaa
    15841 catcgcctgg ccgttgtaga ggggctcagc ctcccccacg ctccccggtt catcaacgat
    15901 gccttcgcta gccaagcctg gcaacgcctg gaaggacagg aattgaacgt cccaggggcc
    15961 atgttttatt taccacctcc tcttaaggag ggtccgtgca gaacctgagg aagcgcgttc
    16021 caaccatcct tacgtcggac gaggtcctgg acaaggcctt ccgcaaggtt agcaagatcg
    16081 ataagcaagg gccagacaag gtagagctgg tgaagcggaa gtccatggcc cggatcgctt
    16141 cggccacgga cgtgatctcc tccaccttcg agaggtatgt gaaggcattc ccaagcatca
    16201 atgaccgcgg cgatttcttg gccgagatga tcgaggtcac ggtgggggtc gaccaactga
    16261 agaaggcact ctccaacctg gactgggcgg cgtccaaggt caaggagctt cagaggcagt
    16321 atacgagcaa ggtcatggcc tcctcctcgg tgcagggcgc ggacagcgcc cgcaaggcct
    16381 tctatggacg ggcatcctcg gtcgtcaaca aggtcgcccc cgacctgggg ttcctcgcat
    16441 atgcgcgcaa cgagttccgc aaggtccccg acatcgacga agggctgctg acgttcgtca
    16501 tcgccggctt ccccaacgtc ggaaagagcc aattcctttc gctagtatcc tcggccgaac
    16561 cggaggtggc cgcctatccc ttcaccacca agggcatagg ggtgggaatg cacgaagcgg
    16621 gctaccgccg ctatcaggtc atcgacaccc ccgggctgtt ggatcgggag ctggaggacc
    16681 ggaatcgcat tgagatgcag gcggtgaccg cgctcaagca cctggccaat gccatactgt
    16741 tcatcctcga cccttccgag acctgcggtt atcccctaga caggcagctt cgcctattgg
    16801 aatcgatcaa ggggctgttc ccggacgtcc caatcctcgt ggtggagaac aaggtcgatc
    16861 ttctggacag cggttcggac aggatgaaga tgagcgctgc cacggggcag ggggtccctg
    16921 aggtgatggc cgcgctccgc gattcggtga gacatctgga gaaggctgaa ccgttgttct
    16981 aggcaaacct tttaattttg tctcccccct agagcgcaag gggtgccctt gcaagaactg
    17041 gcctgtagcg gaccgatgcg gagatacttc tcaacgctct tccaagagaa tgagcgatgc
    17101 tacgcattgg ccgccgaggc cagaggaagg gggcttgacc ctagcacgga ggtcgagatc
    17161 ccacaggccg aggacctggc cgccagggtg gagaagcagc tctccgattg gcatgtggag
    17221 ggggtggcag agcgcatcag ggggctgtcc aagcgaatcg agagccgcga ggaggtatcg
    17281 ctttacatcg ccaaggagct ggccaaggag gcggtggccg cccatgaggg gagggagatg
    17341 gccatcgaaa gggcggtccg ggtgggcctc tcggtcctca ccgaggggat cctggtcgcc
    17401 cccatcgtcg gcatcgcagg ggtgaagatc aatcccaatc cagacgggac cgagtatcta
    17461 tcggtcttct tcaacggccc tatccgggca gcggggggga cggcccaagc gatgagcgtg
    17521 ctcatcgccg acgtggtgcg ccgggagttc ggtatagggg cgtataagcc caccactggc
    17581 gaggtggaac ggctgaagga ggagatcccc ctctacaaac agtgtcagca tctgcagtat
    17641 acgccttcca acgaggagat cgagatcatc taccggaatt gcccggtgtg cgtggacggg
    17701 gagaagacgg aggacatcga gatctccggg ttccggaacc tgccccggat caagaccaac
    17761 gccgtgcgcg gaggcgtatg ccttgtcatc gccgaggggc tatgcctcaa ggcccccaag
    17821 ttggagaagc acgtgaggaa gttggggctg gagggctggg acttcatcag tgagtacctc
    17881 cgaaggaaga ggggtacggg caaggaggag aatacaaagg tggccccgga ctacaagtac
    17941 ctgaaggaca tcgtggccgg tcggcctgtg atcgggcacc cctcccgtcc aggaggtctc
    18001 aggctccgct atggccgggg aaggaccaca ggcctggcgg ccattgccct caatcccgcc
    18061 actatgtatg ccctggacag ctttctggcg ataggcacgc agataaagat cgagaggcct
    18121 gggaaggcag gggcggtcac cccatgcgat cagctggagg ggccgatcct gctgctggac
    18181 agcggggacc tggtccaggc caacacggtg gcggagttca tgccgtatga ggggagggtg
    18241 aaggagatcg tagatgtcgg ccagatcctc ctcccgttcg gggagttcgt ggagaacaac
    18301 cacgttctca tgcccgccga ctacgatctg gaatggcatc gtgcggaact ccgcagcgcc
    18361 aatggggggg agcttccgga aggatgggag gacccaagct ttgattccgc cctggcaatg
    18421 tcggcgcgct acggcgtgcc cctgcatccc aagcacaacc tgttctggta cgatcattcg
    18481 atacaggagc ttcaggccct tcggacgcac atcctgcgcg aagggcaggt cgtcgccggt
    18541 tccctcctcg ttccgttgga accggtgtca aagaggaccc tggagaccct cggcgccctg
    18601 caccgggtgg tggacgggaa agtgatcatc gatcagtata ccgacccgtt gttggcaggc
    18661 ctcgggctcc gggggaagga cggggtggag gacctgaccc ctctggaggg ggcgtcggtc
    18721 atggaggcgg tgtccaggtg cacgggggtc aaggtgaacc cccgctcggt gacccgcata
    18781 ggctgccgca tggccaggcc cgagaaggcc aaggaccgga gcgcgaagg
//
LOCUS       DAOSYN010000003        18521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2481_length_18521_cov_4.427109, whole genome shotgun sequence.
ACCESSION   DAOSYN010000003 DAOSYN010000000
VERSION     DAOSYN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 18521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18521
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_18521_cov_4.427109"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..815)
                     /locus_tag="PLJ11_00200"
     CDS             complement(18..815)
                     /locus_tag="PLJ11_00200"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOO03127.1"
                     /translation="MSVNLNIPDAPRIAKRTLELLKGHEVLLEREMAERFKVPGHLLE
                     EMDVDIMVTVGGDGTILRALQHSKAPIFGINAGDLGFLTVVSEEELEEGIENLLKGNY
                     ELEERIKLQTTVGGRRMPDATNEAVLHTAHVAKIRHFQVFVDGEMAMNVRSDGIVVAT
                     PTGSTCYAMSLGAPLIDPRVEAFVIVPMAPFKFSARPVVVPADSRIAVEVVRPKECVV
                     VVDGQQELPMAGNETVEFRLAPDRARFVSFGKGFYARTREKLMGTLC"
     gene            complement(824..1597)
                     /locus_tag="PLJ11_00205"
     CDS             complement(824..1597)
                     /locus_tag="PLJ11_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOO03128.1"
                     /translation="MRNTLEWLAREVAGRIDRECAFSERATEIGMGADGTPTCRIDKV
                     AEDLILQLMDERKVPLNVLSEEAGLIDRGYDRTLVLDPVDGTYNSLMGIPLYSVSMAV
                     CTRSMNDATHGIVRNLVTGDTFYAEKGKGATWNDRPIHVRRCERDGSVLFVYLGKYSA
                     ATANGVLRKARRTRSLGCSSLEMCLVAAGLADGFYLHSEVYQRALRVVDIAASALVLR
                     EAGGEVYDLAGNVLDMDLDLKVRANCLAVGDRAVLGWVL"
     gene            2011..2922
                     /locus_tag="PLJ11_00210"
     CDS             2011..2922
                     /locus_tag="PLJ11_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03129.1"
                     /translation="METDIIHEHGEQKDWNEGFYFSFYDRGNDICGFMRLDLRPNRNE
                     KTLHCFLMMPDGSLVGTKESIEFDGPTLSAKGVAFTKVEDEKVWNLSFAGELPSYRKG
                     EDGTAKVSFDLRFEGLSPVFDYRECASSDKETLAAAVGSEHLEQMGKVLGSLSVDGRE
                     YYVKGLGERDHTWGEKDWTAPRGWVWLACQVNEGFGFNVTKLYLDKGEYDAGFLYMDG
                     ETLPVVKVDMVTEYDADGSPNTLFMAMYDKEGVVYGVKGEVKKKAVLELKDGGDRGSI
                     LFETLAKYRIDDGDDAGYGIAEYLFRK"
     gene            3538..4212
                     /locus_tag="PLJ11_00215"
     CDS             3538..4212
                     /locus_tag="PLJ11_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HOO03130.1"
                     /translation="MGEAKQRIVPTGFPGVFRSGDMLLTKSLAPGMKVYGERLFSQEG
                     EEYREWVPDRSKLSAYLRRGGSRFPFRPDSHVLYLGASSGTTPSHVSDIVPQGRVYCV
                     EISPRMFRDLVGVCERRPNMMPILGDATKLDDLAFLVERADVVYQDVAQRNQAGIFAK
                     AMKRFSAPYGMLAVKARSEDVAREPMMIFHEAEVLLRKEGLTVLEIIDLEPFEKDHAM
                     IVAGRP"
     gene            4487..4867
                     /locus_tag="PLJ11_00220"
     CDS             4487..4867
                     /locus_tag="PLJ11_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOO03131.1"
                     /translation="MVIQLPNVYAIGFKGDRFLMVYNPKRGGWEMPGGKLEEFETPLE
                     GIKREYREECGFELDVLDCMPMGPVVVFAGILKENRGEAEMGWELMDELPHDLAFPEC
                     EYREQIAWAREAVRNRLGKGLTFK"
     gene            4945..5403
                     /locus_tag="PLJ11_00225"
     CDS             4945..5403
                     /locus_tag="PLJ11_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOO03132.1"
                     /translation="MARMHARRRGSSRSRRPLLTESPEWVPLSNEEIVQMVVRLGKEG
                     MTTSKIGVVLRDQHAVPSVKLATGRSILEILNENGLSPKLPEDLMALMRKAINLNLHI
                     QNGNRGDVSNRRGLQLVESKIRRLVKYYKRTDVLPADWNYSLKNAELLIE"
     gene            5411..6769
                     /locus_tag="PLJ11_00230"
     CDS             5411..6769
                     /locus_tag="PLJ11_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOO03133.1"
                     /translation="MDGSVLPEPLLLRFSSAAAAVRDCTEARVFSHNDADGICTAGVL
                     TSMLMRADKGVQTTMLKGLDLDAIRSGLVPGIDLLIVADMGSSALASLEGLGTKVVVL
                     DHHRPERDSDALVHLNPSVLKVDGARYACASTLSMLLAVTVDERNWDLLPIAFAGMVG
                     DRQHLQGLMGVNAHLFSEAIKKGLVQERKGTLLPPGPIGEGLRRSPHPFIRGVSGDPQ
                     GIAMLLQEARVRPDATWEGLDEADRRRLSSLVALRLLEQGCSLGGLEEELAPDYSFPQ
                     DGLRASQLTRLVNACGRKEETALGLAMLLGEKDARERAEALLKDYEEMLLGALKRTAE
                     RGLSKLRAVQHFESPYREVSSELCGLVMQWVGDQDKATVCLTRMDMEIKVSARGTRRL
                     VDERGLDLSVAMRDAAASVGGVGGGHNIAAGATIPLGKDIDFLQALDSIVAGQMERVS
                     AK"
     gene            complement(6756..7736)
                     /locus_tag="PLJ11_00235"
     CDS             complement(6756..7736)
                     /locus_tag="PLJ11_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HOO03134.1"
                     /translation="MYCLGIEGTAHTVGVGIVDHEGNVLANELEMYRPAEGGIHPREA
                     ANHHAERLVPLVRQAMEEAGLVFKDIGLVAFSRGPGLGPCLRTVATGARALSLSLGVP
                     IVGVNHCISHLEIGRIRTEARDPVLLYASGGNTQIIAFANGRYRIFGETLDIGIGNML
                     DKLGREIGLGYYAGPKIESAAARGKRLLSLPYSVKGMDMAFSGILTAAVAHRRKGEGI
                     EDICFSVQETCFAMLTEVTERAMAHVEKEEVLLGGGVVQNKRLQCMVQDMAEARGARM
                     FVPDRTLCMDNGAMIAWTGLIMHRAGISHPLEATTVDQRFRTDQVEVTWR"
     gene            complement(7751..7981)
                     /locus_tag="PLJ11_00240"
     CDS             complement(7751..7981)
                     /locus_tag="PLJ11_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03135.1"
                     /translation="MTEIILFTKPDCKKCEYVKTHLPEGVHPRMVNTTEAEGLAEAAY
                     YELLNRNLPILVVDDEVYEGSIVILNKLKELA"
     gene            8111..8183
                     /locus_tag="PLJ11_00245"
     tRNA            8111..8183
                     /locus_tag="PLJ11_00245"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8144..8146,aa:Val,seq:gac)
     gene            complement(8552..9250)
                     /locus_tag="PLJ11_00250"
     CDS             complement(8552..9250)
                     /locus_tag="PLJ11_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOO03136.1"
                     /translation="MRQISNPVRQKILSELQRRDLSLSEIAVLTGKAQSTLSVHLDKM
                     VKEGLIGYRDDPEDNRRKIFYIISRPMGSSVVPREELKKAVSNTIGRSIGTPSTFLKG
                     TIRSIVIGMEAIGFNMDPVLKDIGRQMGAEISTRMKANDVEGLIKEIKDFYMVHELGE
                     VVVFSLHPVTLIIRDEYDCSKAPDVGKTLCLLNEGILESIFEERLHMPLRVVEKDTFG
                     AGYNMCKYVIEPSL"
     gene            9415..10425
                     /gene="mtnA"
                     /locus_tag="PLJ11_00255"
     CDS             9415..10425
                     /gene="mtnA"
                     /locus_tag="PLJ11_00255"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOO03137.1"
                     /translation="MKLRIDGKVSEVRAIWCDEGVVRMIDQRILPHRVEVLGFRDHHQ
                     VAEAIRDMAVRGAPAIGVAAAYGMMLAANNGEDLDAAAKELKATRPTAFDLFFAVDHM
                     LARVREGEDPTEAAEEYANTIVERCRAIGEHGEHLIKDGARIMTHCNAGALATVDHGT
                     ALAPIREAWRNGKKVFVYVSETRPRLQGMKLTAFELLNEGIPHAIIPDGASGHFLREG
                     VDIVIVGADRIAANGDFANKIGTFEKAVLAKELGVPFYTAAPLSTFDLSLKRGEDIPI
                     EHRGEEEVTMMDGMRIAPQGCKALNPGFDVTPAKYVTGIITERGVLRPEDIARRLRGA
                     RL"
     gene            10422..11039
                     /locus_tag="PLJ11_00260"
     CDS             10422..11039
                     /locus_tag="PLJ11_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOO03138.1"
                     /translation="MKQAGMDVVRYGRLLYEKGLTSGTGGNISARVGDVMMITPSGTC
                     KGMLDPTELVTVDIATGRAVGGRPSMETPFHLTVYRERPDVRGVVHTHPRFCTALACA
                     GMTVDPALTPEGLMVLGRKVPLVPYATPGTEALAEALRREVGGANAFLLASHGAISVG
                     KDLKDAFHRMETLEAHAELQYRLALLGKGRSLSEEEAERVLRERA"
     gene            11036..12109
                     /locus_tag="PLJ11_00265"
     CDS             11036..12109
                     /locus_tag="PLJ11_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal biogenesis protein"
                     /protein_id="HOO03139.1"
                     /translation="MILVTKWFGVFLCEGEEVRRYKLFEKDPKLVARKLAAVQRGEVL
                     PEERELAVRRMKVADPRLKALGKPAVFDSSFIRPEAYGLTAEHMQRVMLELGKIRVRE
                     PLSEDRSIAQAIRATDDLIEEINLLSERLHEWYGVYFPELGDLAKDAEYARLVCELGE
                     RRAILDHLGVALESVGTDLVPRDLEVIQGVARTLLELYRRKEALDAYILEGMERVAPN
                     LSALLSPNLAARLIALAGGLQRLARLPSSTVQLLGAEKALFLHLRSGKRPPKHGVIFQ
                     HPWVNRSPYWQRGKVARSLGAKISIAARVDAYHGEPIADQLKEQMERRIAEIRERYPD
                     PPRRERSQQRPGQGHQRPQGRRR"
     gene            12152..12538
                     /locus_tag="PLJ11_00270"
     CDS             12152..12538
                     /locus_tag="PLJ11_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HOO03140.1"
                     /translation="MSWTQQEVRELKEGRYMLIDGEPCKIISISTSKPGKHGEAKSNI
                     EAIGVFDNKKRSIVHPVKHKVQVPMIDKRKAQVLSITGDEVQLMDLETYETFHLPIPE
                     EYKGQITAGEEIMYMVAMDRRKITKV"
     gene            12554..13405
                     /gene="speB"
                     /locus_tag="PLJ11_00275"
     CDS             12554..13405
                     /gene="speB"
                     /locus_tag="PLJ11_00275"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOO03141.1"
                     /translation="MARSDAFALANSSFEEAEFVIIGVPYDGTTRLRSGTRKAPAAIR
                     KASRLFEPYLFEHALDTSAVKVHDAGDIDCGQACEDMFRAVEREAASAVRAGKFPILM
                     GGERSITYPFVKAFRDIGVISIDAHLDFHPQGDKRFDHACTMRRVADEVGVENVVVFG
                     VRSMSREELAQELPNYVDAFQIREVGAVASWKRVLNMMRRERVLLSLDMDSVDPGFAP
                     GTSRPEPFGLSGYEVKRCIDMLGDRMVGFDVSEVLPSHDPGATAALAARMIMEVIAMV
                     HTRRGER"
     gene            13415..13984
                     /locus_tag="PLJ11_00280"
     CDS             13415..13984
                     /locus_tag="PLJ11_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOO03142.1"
                     /translation="MDLAVLISAFVLISLTELGDKTMIAVITLSSKYPKRATFLGSYI
                     GLAAVTAVGVLIGEVLFQHVPPWIVGLAAGAVFILFGVVTLLRKEEEGAEVRGNGRWG
                     GLLTSFGFVALMELGDKTQLSVIALSAESGAALMVFLGAALGFLWLTVLEVALGKAIG
                     EKVPKDYIRIGSGLLFIAFGIIFLVQQLL"
     gene            complement(13985..15334)
                     /gene="glmM"
                     /locus_tag="PLJ11_00285"
     CDS             complement(13985..15334)
                     /gene="glmM"
                     /locus_tag="PLJ11_00285"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOO03143.1"
                     /translation="MTRLFGTNGVRGIVNQDMSVEFALEVGRAIGTFMGGRVAIATDS
                     RTSGDMLRSAVSAGIVSAGSDVLDLGMLPTPALQYYVKNSGAKGGVMITASHNPPEFN
                     GIKCVDHDGTEMPRRKEEVIERIYSERDFALPAWNAVGRIARMDGVASSYTNAIRRAV
                     DRFKVQEAGLTVVLDCANGAGSITSPGLLEALGVRAITLNCNPQGTFPGHPSEPTPEN
                     LRDLVAAVKATGAALGIAHDGDADRAIFVDDKGTYVPGDQSLTVVASHIVQEHGGGTV
                     VTPVSTSSCLEEVVRRHNGQVIYTKVGAPTVARRMMEIGAVFGGEENGGLIFPEHQYC
                     RDAAMAIAKMLEIIALKGPLSELLKEVPCRSLDKRKLPCPDDKKGWVLEQVEAYFADR
                     RTDLTDGVKVYFEEGWSLMRPSGTEPIFRIYSEGKDEATAKRCGDECEKVVRELLEE"
     gene            complement(15339..16025)
                     /locus_tag="PLJ11_00290"
     CDS             complement(15339..16025)
                     /locus_tag="PLJ11_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOO03144.1"
                     /translation="MSERTSLLEWFYSRRESMVFRNMRGHAQDVGDTIIEFNRAMSAL
                     CRGEKEQALDALKRSFLAEKAADYREQAMSEELSRGDLEEKNREDLMHLARRMDYAAD
                     WCKEAGMNLQLVLEIGIPVPPAIWSKFCQMSRELERMAKALKVCVDNLGVNNDLVLRA
                     RKEIEDSEHVMDDMYFAMKKEFFLSDLDARALYLMRDMLHGVENSADNFKDSADIIHI
                     LIVAQRDRPR"
     gene            complement(16018..17103)
                     /locus_tag="PLJ11_00295"
     CDS             complement(16018..17103)
                     /locus_tag="PLJ11_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO03145.1"
                     /translation="MPEVRLEGLGKRYGNLTAVNNVHLTVHDGEYVTVLGPSGCGKTT
                     LIRMIAGIIEPTEGKVYIDGRDMAGVPIEDRDIGYVFQNIALFPHLTVKDNVSYGPRA
                     RALPPEEQLRVSRRFLELVRLLDRMGMFPDELCGGEQQKAALARALASGSRLLLLDEP
                     LSALDSRVRVDLRYELRRLAKELGLTTIHVTHDQEEAQSVSDRIVLMRAGAIVETGTP
                     EELYARPKHIFTANFIGETNLLEGWVVEIRDGISVIELRNGGKVEVHQTDHSVGDAVV
                     ISVRPEFMYPADEGMRAVVRGITYMGTYWRVKAETEDDEEVDFDMATLDGNLLTIGKD
                     VYLAVNPKAAVLYPRPEDGVEEAIRLE"
     gene            complement(17103..18194)
                     /locus_tag="PLJ11_00300"
     CDS             complement(17103..18194)
                     /locus_tag="PLJ11_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO03146.1"
                     /translation="MPSVRLIGLRKAFGGIKAADGLDLEVRNGEYLCLLGPTGAGKTT
                     ALRMIAGLVSPDEGKVLFNGEDVTNTPVEERKAALLPQTYSLFPQLTVEENITFGLDI
                     RGVDDRLKEQVLISMLDLVRLSGRSTAFPRELSGGMQQRTALARALASGADMLLLDEP
                     LRALDARLRISLRTELRSLVKSLRMTTIHVTHDQDEALVMADRVAIIREGRIIQVGTP
                     KEVWDRPATPFVANFLGQSNFFIGEVLQSSPCTLLRTPDGTVLKGRSSDLIPGTQAVM
                     GVKIGSTELSKERPGYLQAKVERILFEGRNLHMDMMTGGGQRLSAKLPSWKKGRITVG
                     DTVGIYWEEEKGTVFSMPANGLESELKVE"
     gene            complement(18194..>18521)
                     /locus_tag="PLJ11_00305"
     CDS             complement(18194..>18521)
                     /locus_tag="PLJ11_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO03147.1"
                     /translation="EEVDPAYEETARTLGARPVQAFRRVLYPMVKSSILAGAIMAFTR
                     SLGETGATQAVVANAQTAPVYIVDLINLETAAGYYSAGLACIMLIVISFLFMLALRYV
                     TNRRAR"
BASE COUNT         3312 a         5847 c         5977 g         3385 t
ORIGIN      
        1 gtcgatgccc ctgaccttca gcatagggtc cccatcagct tttccctggt gcgggcgtag
       61 aaccccttcc cgaagctgac gaaccgtgcc ctgtccgggg cgagcctgaa ctccacggtc
      121 tcgttcccgg ccataggaag ctcctgctgg ccgtcgacca ccacgacgca ctccttggga
      181 cggaccacct ccacggcgat gcggctgtcc gccgggacca ccaccggccg ggcggagaac
      241 ttgaaggggg ccatcgggac gatcacgaag gcctccaccc tcgggtcgat caacggcgcc
      301 ccgaggctca tggcatagca ggtggaccct gtcggggtgg ccaccactat gccgtccgaa
      361 cggacgttca tggccatctc cccgtccacg aatacctgga agtggcggat cttggccacg
      421 tgggccgtat gcagcaccgc ctcgttcgtg gcatcgggca tccttctgcc gcccaccgtg
      481 gtctgcagct tgatgcgctc ctccagctcg tagttgccct tgagcaggtt ctctatgccc
      541 tcctccagct cctcctcgga gactaccgtg aggaacccca ggtcccctgc gttgatgccg
      601 aagatgggag ccttgctgtg ctgcagcgcc ctgaggatgg tgccatcccc tccgacggtc
      661 accatgatgt ccacgtccat ctcctcgagg agatgacccg ggaccttgaa cctctccgcc
      721 atctcccttt ccagcagcac ctcgtgtccc ttcagcagct ccagggtcct cttggcgatg
      781 cgcggggcgt cgggaatgtt caagttgacg ctcatcccga acctcatagg acccacccca
      841 gcacggccct gtccccgacg gcgaggcagt tagcccggac cttgaggtcc aggtccatgt
      901 ccaatacgtt cccggcaagg tcgtagacct cgccccctgc ctcccggagg accagggcgc
      961 tggcggcgat gtccaccacc ctcagggccc tctggtacac ctcggagtgc agatagaagc
     1021 cgtcggccag gcctgccgcc accaggcaca tctccagcga ggagcacccg agggacctcg
     1081 tcctcctggc cttgcgcagc acgccgttgg cggtggcggc ggagtacttg cccaggtata
     1141 cgaagagcac cgagccgtcg cgctcgcacc tgcggacgtg gatgggccgg tcgttccagg
     1201 tggccccttt ccccttctcg gcgtagaagg tgtcccccgt gaccaggttc ctgacgatgc
     1261 cgtgggtggc atcgttcatg ctcctggtgc acaccgccat ggaaacgctg tagagcggta
     1321 tgcccatcag ggagttgtag gtcccgtcca ccgggtcgag caccaaggtc cgatcgtacc
     1381 cgcgatcgat gagcccggcc tcctcgctaa ggacgttcaa ggggaccttc ctctcatcca
     1441 taagctggag gatcaggtcc tcggccacct tgtctatgcg gcacgtgggg gtcccatcgg
     1501 cccccatccc gatctccgtc gcccgttcgc tgaaagcgca ctctcggtcg atcctgccgg
     1561 ccacctctct ggccagccat tccaatgtat tcctcatccc gatggatatt ggcggggggc
     1621 tattcataat tgcgccggtg gcggcgaagg caccgaagcg cactggtagg ccctttgcaa
     1681 ggtctcctgt gcctgtcctt cggagtgggg ccgcttggcg gccgtcccgg ttccgggaaa
     1741 gggacggccg aagccgctag gaggtgccat cggggccttt cgaggcgccg accgatggcg
     1801 gggggccgcc caggggacgg ggaggacgga catcctcgga ccgatggggc ggcatccaga
     1861 caggctacgt agagcggcgg atgtgtccag gggagacggt gcgcagaagg tcgccccgga
     1921 gaggaggggg acgaaaatct tcataacggg tcagcggata ccccttgacg gcaacgaagg
     1981 cccgaactat agtaaggaag gatgatccct ttggagactg acattattca cgaacatggc
     2041 gaacagaagg actggaacga gggcttctac ttcagcttct atgaccgcgg gaatgacatc
     2101 tgcggattca tgcgcctaga cctgagaccc aatcggaacg agaagacgtt gcactgcttc
     2161 ctcatgatgc ctgacggttc cttggtgggc acgaaggaga gcatcgagtt cgacggccct
     2221 acattgagcg cgaagggcgt cgccttcacg aaggtggagg atgagaaggt ctggaacctt
     2281 tcgttcgccg gcgagcttcc ctcgtatagg aagggagagg acggcaccgc caaggtcagc
     2341 ttcgacctgc gcttcgaagg cctgagcccg gtgttcgact accgcgagtg cgcgtcctcc
     2401 gacaaggaga ccctggcggc cgcggtcggc tccgagcacc tggagcagat gggcaaggtg
     2461 ctgggcagcc tgagcgtgga tggacgcgag tactacgtca aggggctcgg ggagagggac
     2521 catacctggg gagagaagga ctggacagct ccgcgaggct gggtctggct tgcatgccag
     2581 gtcaacgagg ggttcgggtt caacgtcacc aagttgtatc tggacaaggg agagtacgac
     2641 gcggggttcc tttatatgga cggggagacc cttcccgtgg tcaaggtgga catggtcacc
     2701 gagtacgacg cggatggcag ccccaacacc ctcttcatgg ccatgtacga taaggaagga
     2761 gtggtctacg gggtgaaggg cgaggtcaag aagaaggccg ttctggagct caaggacggg
     2821 ggcgaccgcg gctcaatatt gttcgagacc ctggccaagt atcgcatcga cgacggggac
     2881 gatgccggct acggcatcgc cgagtacctg ttcaggaagt gaccctcacc tccctgacca
     2941 tcctggtcag ggagatgcag gcgaggtaca ggccggccat ccccccactc ccaaggccgg
     3001 cacctggccc atggaccgcc catggcgggt catccctccc tattgccggt tccggggcga
     3061 gcgccgagcg gcggtccttc ttttaaccgc ccatcatgtc cttgcgcgtc cctccgaggc
     3121 ctaggtcgga cgttcattcc atgatgatcc agatggccgt tcgtcgcctg ccgccgcaat
     3181 gccccttcct cacgatgcgc tagggcgccc ttccccgtcc gccctttagc ccttaagtga
     3241 gttgtggtgt tccgaggcgg gacgatcgac gatgctcaca tgcccatggg gaatgaccca
     3301 tttccctccc gaggggacga tggccgatcg accattccgc cttgggcaga agcctgttga
     3361 ccgtagccgc ctgtcggtcc ctggaacgat gccgatcgag cggcccggag ggaggggagc
     3421 agcaggatgg caccggcccg atggccgatg ggccctcttc gggatcggta cgacctcctt
     3481 ccacgctccg cggaggggcg gtgaaacgct ttataatctc tcggccatgg ccggaccatg
     3541 ggcgaggcca agcagaggat agtgccgacg gggttcccgg gcgtcttccg ttccggggac
     3601 atgctgctca ccaagagcct cgcccccgga atgaaggtgt acggcgaaag gctgttctcc
     3661 caggaggggg aggagtacag ggagtgggtg cccgaccgga gcaagctctc cgcctatctc
     3721 cgccggggcg gttcccgctt ccctttccgt cccgactccc atgtgctcta cctgggggcc
     3781 tcgagcggca ccacccccag ccacgtttcg gacatcgttc cccagggaag ggtgtactgc
     3841 gtcgagatat cgccgcgcat gttccgggac ctcgtaggcg tttgcgagcg ccgcccgaac
     3901 atgatgccga tcctggggga cgccaccaag ctggacgacc tggccttcct ggtggaaagg
     3961 gccgacgtgg tctaccagga cgtggcgcag aggaaccagg cggggatctt cgcgaaggcc
     4021 atgaaacgat tctccgcgcc ctacggcatg ctggccgtca aggcgcgctc ggaggacgtc
     4081 gcccgggagc ctatgatgat ctttcatgag gccgaggtgc ttttacggaa ggaggggcta
     4141 acggtcctgg agatcatcga cctggaacct tttgagaagg accatgccat gatcgttgcg
     4201 ggaaggccct gacgatcccc cgcttctcga ggcacttgaa gatactatca tcgtgcctgc
     4261 atccgcctgt ggcagggcat ccgcgcgtac cccatggaag cccgcatccc gacggcccta
     4321 tgctgaagag cggcgctctc catgaaaggg acggaatggc ccggatccag ggccttgcgt
     4381 tccacggcct acatgacagt gggccctccc ccgtcatcac ctatgcactt ggcgatggga
     4441 atcggataaa tagacatagc tcctctcagg gcgcgttgcc aatgaagtgg tgatccaact
     4501 gcccaatgtc tatgccatag gtttcaaggg ggaccggttc ctcatggtct acaacccgaa
     4561 gcggggagga tgggagatgc ccggcgggaa gctggaggag ttcgagaccc cccttgaggg
     4621 gataaaacgg gagtacaggg aggagtgcgg cttcgagctt gacgtcctcg attgcatgcc
     4681 aatggggccg gtcgtcgttt tcgctgggat cctaaaggag aacagggggg aggcggagat
     4741 gggctgggag ctgatggacg agctgccgca tgacctggcg ttcccggagt gcgaatatcg
     4801 ggagcagatc gcatgggcca gggaggctgt gcggaacaga ctggggaagg gtctaacttt
     4861 taaatagtag gagtgaaata gacggtcact tcgccgccac caacgaagca aacgccttcg
     4921 atatcaaaac aggatgatgt tcatatggca agaatgcacg ccaggagaag gggttcctcc
     4981 cgttcgagga ggcccctgct gaccgagagc cccgagtggg tcccgttgtc aaacgaagag
     5041 atagtccaga tggtggtcag gctgggaaag gagggcatga ccacctccaa gatcggcgtg
     5101 gtcctcaggg accagcatgc ggtgcccagc gtcaagctgg ccaccggcag gtccatcctg
     5161 gagatcctca atgagaacgg cctttccccc aagctcccgg aggacctgat ggctctgatg
     5221 aggaaggcga tcaatctgaa cctgcatatc cagaacggaa accggggcga cgtgtccaat
     5281 cgccgcggcc tgcagctggt ggagtccaag atcaggcggt tggtgaagta ctacaagcgc
     5341 accgatgtgc tgcccgcgga ctggaactat tccctgaaga acgccgagct gctcatcgag
     5401 tgagggggac atggacggca gcgtcctccc tgaacctctt ttgcttcgct tttcctcggc
     5461 cgcggccgcg gtgcgcgatt gcaccgaggc ccgcgtcttt tcgcataacg atgccgacgg
     5521 catttgcacg gcgggcgtgc tcacgtccat gctgatgcgc gctgacaagg gggtgcagac
     5581 cacaatgctc aagggcctcg acctggacgc catccggtcg gggctcgtgc cgggcataga
     5641 cctgctcatc gtggccgaca tgggatcgag cgccctggcc tcattggagg ggcttgggac
     5701 caaggtcgtg gtgctggacc atcatcggcc ggagagggac tcggacgcgc tcgtgcacct
     5761 gaacccgtcg gtgctcaagg tcgatggcgc cagatatgcg tgcgcatcca ccctttccat
     5821 gctgctcgcc gtgacggtcg atgaacggaa ttgggacctg ctgcccatcg ccttcgccgg
     5881 catggtcggc gaccgtcagc atctccaggg gcttatgggc gtcaacgccc atctgttctc
     5941 tgaggctatc aagaagggac tggtccagga gaggaaaggg acactcctcc cgccgggtcc
     6001 gataggggag gggttgaggc gctcccccca tcccttcatc cggggggtca gcggggaccc
     6061 ccagggtata gccatgctgc tacaggaggc gcgagtgcgc cctgacgcca cctgggaagg
     6121 actggacgag gccgacagac gacggctctc gtcccttgtc gccctgcgcc tgctggagca
     6181 gggatgttcc ctcggcgggc tcgaggagga actcgcccct gactattcct tcccccagga
     6241 cggtctccgc gcctcgcagt tgacgagact ggtcaacgcc tgcgggagga aggaggagac
     6301 cgctctaggg ctggcaatgc tgctggggga gaaagatgcc agggagcgcg ccgaggcctt
     6361 gctgaaggat tatgaggaga tgctgttggg agcgctcaag cggaccgcgg agcgcggcct
     6421 ctccaagctg cgggcggtcc agcacttcga atccccctat agggaggtgt ccagcgagct
     6481 gtgcggcctc gtgatgcagt gggtggggga ccaggataag gccaccgtct gcctgacccg
     6541 gatggacatg gagatcaagg tctctgcccg cgggacacgg aggcttgtgg acgaacgtgg
     6601 gctggacctt tccgtggcca tgcgggacgc cgccgcctcc gtgggcgggg tgggcggggg
     6661 gcacaacatc gccgctgggg cgaccatccc attgggcaag gacatcgatt tccttcaggc
     6721 cctggactcg atcgtggccg ggcagatgga aagggtcagc gccaagtgac ctccacctga
     6781 tcggtgcgga accgctggtc gacggtggtc gcctccaggg gatggcttat ccccgcccgg
     6841 tgcatgatga ggccggtcca ggcgatcatg gccccgttgt ccatgcacag cgtcctgtcg
     6901 ggaacgaaca tgcgggcccc gcgggcctcc gccatgtcct gcaccatgca ctgcaggcgc
     6961 ttgttctgca ccaccccccc gccgaggagc acctcctcct tctccacgtg ggccatagcc
     7021 cgctcggtga cctcggtgag catggcgaag catgtctcct gcacgctgaa gcagatgtcc
     7081 tcgatgccct cccccttcct ccgatgggcc accgcggccg tgaggatgcc agagaaggcc
     7141 atgtccatgc ccttgaccga gtaggggagg ctgaggagcc tcttcccccg cgcggcggcg
     7201 ctctctatct tggggccggc gtagtacccg aggccgatct ccctccccag cttatcgagc
     7261 atgttgccga tgccgatgtc cagcgtctcc ccgaatatgc ggtagcggcc gttggcgaag
     7321 gcgatgatct gggtgttgcc gccggaggcg tagaggagca cggggtccct ggcctccgtc
     7381 ctgatccttc cgatctccag gtgggagatg cagtggttca ccccgacgat cgggaccccc
     7441 aggctcaggg agagggcccg ggcgcccgtg gccacggtcc gcaggcaggg ccccaggccc
     7501 gggcccctcg agaaggccac caggccgatg tccttgaaca caagccccgc ttcctccatg
     7561 gcctggcgca ccaaggggac cagcctctcg gcgtgatggt tggcggcctc ccggggatgg
     7621 atgcccccct cggccggccg gtacatctcg agctcgttgg ccagcacgtt cccctcatgg
     7681 tccacgatcc ccacgcccac ggtgtgggcc gtgccctcga tccccaggca gtacatacca
     7741 gtccttggcc tcaggccagt tccttgagct tgttgaggat gacgatggac ccctcataga
     7801 cctcgtcgtc caccacgagg atggggaggt ttctattgag gagttcgtag tatgcggcct
     7861 cggcgagtcc ctcggcctcc gtcgtattga ccatgcgggg atggacgccc tctggcagat
     7921 gggtcttgac atactcgcac ttcttacaat cgggtttggt gaagagaatg atctccgtca
     7981 tttagctcag aggatgcaaa gggcggtcac taataaaatt tactttcgaa ggaagggcac
     8041 cagcctatgg gtcgagcgct catgaactac gaaaggaata tcaattagaa cggcttacgg
     8101 ccaatcccaa gggctcatgg tctagcggtt atgacgtctc cctgacacgg aggaggtctc
     8161 cggttcaaat ccggatgagc ccatccaaat tccttggtaa tgccacgagc tgcctattcg
     8221 atgacgccat catagatgcc ggttcattag gtggatgaac ttgaagaaga cggaagctcg
     8281 tcctatagaa tggaaaccgc aaggatcgta gagaacagtc aagaaaaggt ccgatcgggt
     8341 cctatcatcg ccgcgataga ggacggccct ctgaggattg gacgaaagat agggcgtcga
     8401 ggcatgcgat cttcccgaac ggtagcgccg ttgaggtcca caaacgtttc cgagcgatcg
     8461 taggctgaac taagccttct gccaatgtgc cttaaagagc caggacccga aatggagggt
     8521 gggcccatga agatgttgta ttggaaaagg gtcagaggga tggctcgatc acgtacttgc
     8581 acatattgta gcccgcgccg aaggtgtcct tctccaccac ccgcaggggc atgtgcagcc
     8641 tctcctcgaa gatcgattcc agtatcccct cgttcaatag gcagagggtc tttcccacgt
     8701 ccggggcctt ggagcagtcg tactcgtccc ggatgatgag cgtgacggga tgcagggaga
     8761 acaccacgac ctcccccagc tcatgcacca tgtagaagtc cttgatctcc ttgatgagcc
     8821 cctccacatc gttggccttc atgcgcgtgg agatctccgc tcccatctgc cggccgatgt
     8881 cctttagcac tgggtccatg ttgaacccta tcgcctccat gccgatgacg atggagcgga
     8941 tcgtgccctt gaggaacgta ctcggggtcc ctatggagcg cccgatggtg ttggatacgg
     9001 ccttcttcag ctcctcccgg gggaccaccg agctgcccat tggccgggaa atgatgtaga
     9061 agatcttccg gcgattgtcc tctggatcgt ccctgtatcc gatgaggccc tccttgacca
     9121 tcttgtccag atgcacggac agggtgctct gcgccttgcc agtgaggacg gctatctcgc
     9181 tcaggctgag gtcccttcgc tgcagctcgc tcagtatctt ctggcggacc gggttggaga
     9241 tctgcctcaa cccggtggta gtggaataga cgtcgaagtc cgtggtcctg ctgggcaggg
     9301 atatcgccgg atgaccgatt atggctcggt ctaattaaac ctatcgtaag gatacgaacc
     9361 atcgggccct gcatacctta attagattgc ggcccctcca ctcagcgaac gcagatgaag
     9421 ctcaggatag acggcaaggt cagcgaggtt cgggcgatct ggtgcgacga gggggtggtc
     9481 cgcatgatcg accagaggat ccttccgcac agggtggagg tccttgggtt ccgggaccat
     9541 catcaggtgg ccgaggccat ccgggacatg gccgtgcgcg gcgcgccggc catcggcgtg
     9601 gccgcggcct acgggatgat gctggcggcc aacaacggcg aggacctgga cgcggcggcc
     9661 aaggaactca aggccacccg gcccaccgcc ttcgacctgt tcttcgccgt ggaccacatg
     9721 ctcgcccgcg ttcgagaggg ggaggacccc acagaggcgg cggaggagta cgccaacacg
     9781 atagtggaga ggtgccgggc gatcggggag cacggggagc acctgatcaa ggacggtgca
     9841 cgcatcatga cgcactgcaa cgccggggcc ctggccacgg tggaccacgg gaccgccctg
     9901 gcgcccatcc gcgaagcctg gcggaacggg aagaaggtgt tcgtctacgt ctccgagacg
     9961 cggcccaggc tgcagggcat gaagctcacc gcgttcgagt tgctgaacga gggcattccg
    10021 cacgccatca tccccgatgg cgcctccggt catttcctgc gggagggggt ggacattgtc
    10081 atcgtggggg cggaccgcat cgccgccaac ggcgacttcg ccaataagat cggtacgttc
    10141 gagaaggcag tgctggccaa ggagctcggc gtgccgttct acaccgccgc gccgctgtcc
    10201 accttcgacc tctcccttaa gcggggggag gacatcccga tcgagcacag gggggaggag
    10261 gaggtgacca tgatggatgg gatgcgcatc gcgcctcagg gctgcaaggc gttaaacccc
    10321 ggcttcgatg tcaccccggc caagtacgtc accgggatca tcaccgagag gggagtgctg
    10381 aggccagagg acatcgcgcg gaggctcagg ggggcgaggc tgtgaaacag gcgggaatgg
    10441 atgtcgtacg ctacggcagg ctgctgtatg agaaggggtt gaccagcggc acgggaggga
    10501 acatcagcgc cagggtgggg gatgtgatga tgatcacgcc gtccggcacg tgcaagggca
    10561 tgctggaccc cacggagctg gtcacggtcg acatcgccac tggcagggcg gtgggcggca
    10621 ggccatccat ggagacgccc ttccatctga cggtgtaccg ggagaggccg gacgtgcgtg
    10681 gagtggtgca cacgcatccc cgattctgca cggcccttgc ctgcgccggc atgaccgtgg
    10741 acccggcctt gacgcccgag ggcctgatgg tcctgggaag gaaggtgccc ctcgtcccct
    10801 acgccacgcc tggcacggag gccctggccg aggcgctcag gcgggaggtc gggggggcga
    10861 acgccttcct gctggcgagc catggcgcga tctcggtggg gaaggacctt aaggacgcct
    10921 tccatcgcat ggagaccctg gaggcccacg ccgagctgca gtaccggctt gcgctgctgg
    10981 ggaaggggag gtcgctttcg gaggaggagg cggaacgggt tctgagggag cgagcatgat
    11041 acttgtcacg aaatggttcg gggtgttcct ctgtgagggg gaggaggtcc gccggtacaa
    11101 gctcttcgag aaggacccca agctggttgc ccgcaagctc gcggccgtcc agcgtgggga
    11161 ggtccttccg gaggagcggg agctggccgt ccggcgcatg aaggtggcgg acccccgcct
    11221 caaggcgctc gggaagccgg cggtattcga ttcctcgttc attcggccgg aggcgtacgg
    11281 gctgaccgcg gagcacatgc agcgggtgat gctggagctc ggaaagatca gggttcggga
    11341 gcctctcagc gaggaccgct cgatcgcgca ggcgataagg gcgacggacg acctcatcga
    11401 ggagatcaac ctgctgagcg agcgactgca tgaatggtac ggcgtgtact tcccggagct
    11461 gggcgacctc gcgaaggacg cggagtacgc ccgcctggtg tgcgagctgg gggagcggcg
    11521 ggccatattg gaccacctgg gagtggcgtt ggaatcggtc ggcaccgacc tcgtccccag
    11581 ggacctcgag gtaatccaag gagtcgcccg caccctactg gagctctacc ggcggaagga
    11641 ggccctggac gcctacatcc tggaggggat ggagcgggtg gcgccgaacc tgtcggccct
    11701 gctcagcccc aaccttgccg ccaggctgat agcccttgcg ggaggcctcc agcgtttggc
    11761 gcgcctgccc tcgtccaccg tccagctgct gggggcggaa aaggccctgt tcctgcacct
    11821 gcgctcgggg aagaggccgc cgaagcacgg ggtgatattc cagcatccct gggtgaaccg
    11881 ttccccgtac tggcagcgcg gaaaggtggc gcggagcctg ggcgccaaga taagcatcgc
    11941 ggccagggtg gacgcctacc acggcgagcc catcgccgac cagctgaagg agcagatgga
    12001 acggaggata gcggagatcc gggagaggta tcccgaccca ccaaggcggg agcgctccca
    12061 gcagcgccct ggtcaggggc accagcgccc tcagggccgt cggaggtaag aaaatatata
    12121 aacgccccga tgattccctc ccagggtagc tatgtcgtgg actcagcagg aagtcaggga
    12181 gctcaaggag ggcaggtaca tgctcatcga tggggagccc tgcaagatca taagcatatc
    12241 cacctccaag cctgggaagc atggagaagc gaaatccaac atcgaggcca tcggcgtctt
    12301 cgacaataag aagcgaagca tagtccatcc ggtcaagcac aaggtgcagg tgccgatgat
    12361 cgacaagcgc aaggcgcagg tcctttcgat aaccggggac gaggtgcagc tcatggacct
    12421 cgagacatat gagacgttcc acctgcccat cccagaggag tacaaggggc agataaccgc
    12481 gggggaggag atcatgtaca tggtcgccat ggaccgccgc aagatcacca aggtgtgatc
    12541 ggccagaggc ctcatggccc gtagcgacgc gttcgccctg gccaactcct ccttcgagga
    12601 ggcggagttc gttatcattg gcgttccgta cgacggcacc acccgcctcc gctcaggcac
    12661 ccgcaaggcg cccgccgcca tccggaaggc gtcccgcctc ttcgagccct atcttttcga
    12721 gcacgccctg gacacctcgg cggtgaaggt ccacgatgcc ggggacatag attgcggtca
    12781 agcgtgcgag gacatgttca gggcggtgga gagggaggcc gctagcgccg tgagggcggg
    12841 caagttcccc atcctcatgg gaggggagcg cagcataacc tatcccttcg tcaaggcgtt
    12901 ccgtgatatc ggcgtcatct ccatcgacgc ccatctggac ttccatccgc agggggacaa
    12961 gcgcttcgac cacgcctgca ccatgcgccg ggtggcggac gaggtgggcg tcgagaatgt
    13021 ggtggtgttc ggggtccgct cgatgtcccg ggaggagctt gcgcaggagc tgcccaacta
    13081 tgtcgatgcc ttccagatca gggaggtggg ggcggtcgcc tcctggaagc gggtgctgaa
    13141 catgatgcgg agggagcgcg tcctcctgag cttggacatg gacagcgtcg atcccggctt
    13201 cgccccgggg acctcccgcc ccgagccctt cgggctcagc gggtacgagg tgaagcgctg
    13261 catcgacatg ctgggggacc gcatggtcgg gttcgacgtc tcggaggtcc ttccgtccca
    13321 tgaccccggg gcgacggcgg ccttggccgc gcggatgatc atggaggtca tcgccatggt
    13381 gcacacccgc cgaggggagc ggtgaggggt agcgatggac ctcgccgtgc tcatctccgc
    13441 cttcgttctc atctccctga cggagctggg ggacaagacg atgatcgcag tcatcaccct
    13501 ctcctccaag tacccgaagc gggctacgtt cctaggttcc tacataggtt tggcggcggt
    13561 caccgcagtt ggggtcctta tcggggaggt cctctttcag catgtgcccc cctggatcgt
    13621 cggtctcgcc gccggggctg tcttcattct cttcggtgtg gtcaccctcc ttcgcaagga
    13681 ggaggagggg gcggaggtgc gcgggaacgg caggtggggc gggctcctca cctccttcgg
    13741 cttcgtagcg ctcatggaac tgggagacaa gacccagctc tcggtcatcg ccctctccgc
    13801 cgaatcgggg gcggccctga tggtgttcct gggggcggcg ctcggctttc tctggctcac
    13861 ggtcctggag gtcgcccttg gcaaggccat cggggaaaag gtccctaagg actacatccg
    13921 catagggagc gggctgctct tcatcgcctt cggcataatc ttcctggtcc agcagctgct
    13981 gtgatcattc ctcgagcagc tcgcggacca ccttctcgca ttcgtcgccg cagcgcttgg
    14041 cggtcgcctc gtccttccct tccgaataga tgcggaagat cggctcggtc ccggaggggc
    14101 gcatgaggct ccagccctcc tcgaagtaga ccttgacgcc gtcggtcagg tcggtccgcc
    14161 ggtcggcgaa gtaggcctcc acctgttcca ggacccaccc cttcttgtcg tccgggcagg
    14221 gcagcttccg cttgtccagg ctgcggcagg ggacctcctt aagcagttcg gacagggggc
    14281 ccttgagcgc gatgatctcc agcatcttgg caattgccat ggccgcatcc ctgcagtatt
    14341 ggtgctccgg gaagatcagc ccgccgttct cctccccccc gaacaccgcc ccgatctcca
    14401 tcatgcggcg cgcgaccgtg ggcgcgccca ctttcgtata gatcacctgg ccattgtgcc
    14461 tccggaccac ctcctccagg caggaggagg tgctcaccgg ggtgaccacc gtgccgcccc
    14521 cgtgctcctg cacgatgtgc gaggccacga ccgtaagcga ttggtccccg gggacgtacg
    14581 tccccttgtc gtccacgaat atggcgcggt ccgcgtcccc gtcgtgggcg atgcccaggg
    14641 cggcgccggt ggccttgacc gccgccacga ggtccctcag gttctccggg gtcggctcgc
    14701 tgggatgccc cgggaaggtc ccctgtggat tgcagttgag ggttatcgcc ctcaccccca
    14761 gggcctcgag cagcccgggt gaggtgatgg aaccagcgcc attggcgcag tcgaggacga
    14821 cggtgagccc ggcctcctgg accttgaagc ggtccaccgc gcgcctgatg gcgttggtgt
    14881 aagaggaggc taccccgtcc atgcgcgcga tgcggccgac cgcgttccag gcagggaggg
    14941 cgaagtccct ctccgagtag atgcgttcga tcacctcctc cttccgccgg ggcatctccg
    15001 tcccgtcatg gtccacgcac ttgatgccat tgaactccgg ggggttgtgg gaggcggtga
    15061 tcatcacccc tcccttcgcc ccggagttct tgacatagta ctgcagcgcg ggcgtgggca
    15121 gcatgcccag gtcgagcacg tcggagccgg cggagacgat gccggcggag accgccgagc
    15181 ggagcatgtc cccggaggtg cgggaatcgg tcgcgatggc cacccgtccg cccatgaacg
    15241 tgcctatcgc ccggccgacc tccagggcga actcgacgct catgtcctgg ttgacgatcc
    15301 cccgcactcc attggtgccg aacagtctgg tcatcctatc accttggccg gtcgcgctgg
    15361 gcaactatga ggatgtggat gatgtccgcg gagtccttga agttgtcggc ggagttctcg
    15421 acgccgtgca gcatgtcccg catcaggtag agcgcccggg cgtccaggtc agaaaggaag
    15481 aactccttct tcattgcgaa gtacatgtcg tccatcacgt gctcggagtc ctcgatctcc
    15541 ttgcgggcgc gcagcaccag gtcgttgttc acgcccaggt tgtccacgca gaccttcagg
    15601 gccttggcca tgcgttccag ttcccggctc atctgacaga acttgctcca gatggcgggg
    15661 ggaacgggga tacctatctc cagcacgagc tgcaggttca tcccggcctc cttgcaccag
    15721 tcggccgcgt agtccatgcg gcgggcgaga tgcatgaggt cctcccggtt cttctcctcg
    15781 aggtccccgc gcgagagctc ctcggacatt gcctgctcgc ggtagtcggc ggccttctcg
    15841 gccaggaagg agcgcttcag cgcgtccagg gcctgctcct tctccccgcg gcacagcgca
    15901 ctcatggcac ggttgaactc gatgatggtg tcgcctacgt cctgggcatg cccccgcatg
    15961 ttccggaaga ccatcgattc cctacgcgaa tagaaccatt ccagcaacga cgtcctctca
    16021 ctcaagcctg atcgcctcct ccactccatc ttccggtctg ggatacagta ccgcggcctt
    16081 cgggttcacc gctaaataca cgtcctttcc gatagttaaa aggttcccgt cgagggtggc
    16141 catatcgaag tcgacctcct cgtcgtcctc cgtttcggcc ttcacccgcc aataggtgcc
    16201 catgtacgtg atccctctga cgaccgcccg catcccctca tcagcgggat acatgaactc
    16261 cggacggacc gagatgacca ccgcatcccc tacggaatgg tctgtctggt gcacctccac
    16321 cttcccgccg ttcctgagct caatgaccga tatcccgtcc ctgatctcca ccacccagcc
    16381 ctccagcagg ttggtctccc ctatgaagtt ggcggtgaag atgtgcttcg gcctcgcgta
    16441 cagctcctcc ggcgtgccgg tctcaactat tgccccggcg cgcatgagga cgatgcggtc
    16501 cgacaccgat tgcgcctcct cctggtcgtg ggtgacgtgg atggtggtga gcccgagctc
    16561 tttggccagg cggcgcagct cgtagcgcag gtccaccctg acccgggagt ccagggcgga
    16621 gagcggctcg tcgaggagca gcagccttga accggatgcc agcgccctcg cgagggccgc
    16681 cttctgctgc tcccctccgc acagctcgtc cgggaacatc cccatcctgt ccaggagcct
    16741 caccagctcc aggaagcgcc tcgacacccg gagctgctcc tccggtggga gggcccttgc
    16801 cctcgggcca taggatacat tgtcctttac ggtcagatgc gggaacaggg cgatgttctg
    16861 gaagacgtac ccgatgtcgc ggtcctcgat gggtaccccg gccatgtccc ttccgtcgat
    16921 gtacaccttc ccctccgtgg gctcgatgat ccccgcgatc atccgaatga gggtggtctt
    16981 gccgcatccg gacgggccga ggaccgtgac gtactccccg tcgtggaccg tgaggtggac
    17041 attgttcacg gccgtaaggt tgccataccg ctttccaagc ccctccaggc gtacctcggg
    17101 catcattcca ccttcagctc gctctccaga ccgttcgccg gcatggagaa caccgttccc
    17161 ttctcttcct cccagtatat accaactgtg tcgcccaccg tgatcctgcc cttcttccat
    17221 gaggggagct tggccgagag gcgctgcccg ccccccgtca tcatgtccat gtggaggttg
    17281 cggccctcga acaatatgcg ctccaccttg gcctgaagat atcctggccg ttccttggac
    17341 agctccgtgc tcccgatctt cacccccatc accgcctgcg ttcctgggat gaggtcggag
    17401 gaccggccct tcagcaccgt tccatcgggc gtgcgcagca gggtgcatgg gctggactgc
    17461 agaacctccc caatgaagaa gttggactgt cccaggaagt tggccacgaa gggcgtcgcc
    17521 ggccggtccc acacctcctt tggagtgccc acctgtatta tccggccctc cctgattatc
    17581 gccacgcggt ccgccatgac cagggcctcg tcctgatcat gggtgacgtg gatggtcgtc
    17641 atcctcaatg atttgaccag cgatcgcagc tccgtccgca gggagatccg cagccgcgcg
    17701 tccagggccc gtagcggctc atcgaggagc aacatgtccg ccccggatgc caacgcccgg
    17761 gccagcgcgg tgcgctgctg cattcccccg ctgagctccc gggggaaggc ggtcgaccgt
    17821 cccgacagcc gcacgagatc cagcatgctg atgagcacct gctccttcag cctatcatcc
    17881 accccccgga tatccaggcc gaacgtgatg ttctcctcga ccgtgagctg cgggaacagg
    17941 gagtacgtct ggggaagcaa agcggccttg cgctcctcca caggcgtgtt ggtcacgtcc
    18001 tccccgttga agagcacctt gccctcgtcc ggagagacca gcccggctat catgcgcagg
    18061 gcggtcgtct tccccgcccc ggtcggaccg agcaggcaca ggtactcgcc gttccgcacc
    18121 tctaggtcca gcccgtccgc ggccttgatg cccccgaagg ccttccgcag gccgatcagc
    18181 cgcaccgatg gcatcagcgg gccctcctgt tggtcacata acggagcgcc agcatgaaca
    18241 ggaaggagat gactatgagc atgatgcacg ccaagccggc ggagtagtag cctgccgcgg
    18301 tctcgagatt gatcaggtcc acgatgtaca ccggggcggt ctgggcgttg gccaccaccg
    18361 cctgggtcgc cccggtctcc ccgaggctgc gggtgaaggc catgatcgca ccggccagga
    18421 tggagctttt taccattggg tacagcaccc tccggaaggc ctgcaccggc cttgcgccca
    18481 gggtgcgcgc cgtctcctca taggcggggt ccacctcctc c
//
LOCUS       DAOSYN010000004        18345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2520_length_18345_cov_4.168617, whole genome shotgun sequence.
ACCESSION   DAOSYN010000004 DAOSYN010000000
VERSION     DAOSYN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2520_length_18345_cov_4.168617"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PLJ11_00310"
     CDS             complement(<1..1662)
                     /locus_tag="PLJ11_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOO03148.1"
                     /translation="MPSRRWTILVVATLLLGAGLVLPLENDEACYAPGHGPLWTTEAE
                     GAARPSELAPSLATLASAGASLFNITVSFSYELLDGMETAPCAGAFVQLRLGGTSAFH
                     TVRSGHTDASGSVTFTGLVPGTYMARIEADDDRWARVADGSMSTTPNYHWSTQQFSAD
                     RGMGLEYLIRDEARGAWAIYDAIRDGGQWLHDLTGWERSQVTAVWPEGYWPHSHGNEI
                     HIPSDEHFEGAAWQRDIALHEYAHCVHFELRGGSFPDGAGPDPHYIDSESSPGFAFTE
                     GWAEFFERAVEGDPLRADGSSLESTVFADGPFGHGDAGDMDGVIVEGAVANVLWDLYD
                     GVDPLDRPDGSARGDMVDRQFALLWDIMYYDAPNSLADVKRAWPMRDANITVLFRNAR
                     VPMELDPPRNPDRFQSSHTIGEGSLDSTLSVIWWGARDEGSGVLGYSILLSRDRNATP
                     DLVMDQTGSSYTSHPLSPGTYYLHIRTVDQDGNWAGDVVTVGPFVILEGAVPVDGPMF
                     PEGDVRMAFLGLAIVLAVVCPLLVLVVMMERHQLASRTAPGPHALG"
     gene            1788..2801
                     /locus_tag="PLJ11_00315"
     CDS             1788..2801
                     /locus_tag="PLJ11_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOO03149.1"
                     /translation="MKAALMRSPGVLSVEDVEDPALPEGGVLLRVKACAVCGTDIKML
                     EAGHRDLTYPRVPGHEVTGEVLNAGSSGLRKGDLVQVWPGSACKVCASCRAGMDHLCP
                     QVRIMGFNTDGGMAELLAVDDPSRLVPIGDSDPVLLTLAEPLACCINAQSKLGVGNGD
                     SVLILGGGPMGCLNAFLARHRGADTIMLSEPRTERLWPVPKGLFDHVSEPKGDDVRDM
                     VMQATAGRGADVIIPCTPNVRLDRKMFELLSPGGRLCVFSGPRHEDSPVPIDVRDVHY
                     RELMVVGSYGNASGHCREAVGLLRKGADLSWLFTGRYPLDEVRQAFAHASGRTGQKAV
                     VIL"
     gene            2817..3929
                     /gene="trpD"
                     /locus_tag="PLJ11_00320"
     CDS             2817..3929
                     /gene="trpD"
                     /locus_tag="PLJ11_00320"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOO03150.1"
                     /translation="MMEEQLREFGASVDALIRGEHIGRQRTRDMFREVLLNEQPDLQQ
                     GAFLAALTSAGPTPEEIAGSWEAIFELDTVKARPEVDRVLVENCGTGMDRIKTFNVST
                     LASVVAAAGGVYMAKHGARAITSKCGIVDMAETLGVDVECDASVVRRSVEVAGIGLFN
                     GMSAKVHPQALSRILSQIRFGTILNIAGSLANPASPEVGVRGVYAKELVLPLALTMRE
                     MGYQRGIVAHGLDASGERGMDELSTLGRTIIAEFHGDGEIDSYEITPQAMGLPVAQER
                     EVLSSLDREREAARFIRVLMGKEKGARRDIVALNTAPILYLCGDASSLQEGVGKAMDI
                     LASGKGLDKLRDWVRVQNTGDPGEKLERLDRMAELV"
     gene            complement(3926..4321)
                     /locus_tag="PLJ11_00325"
     CDS             complement(3926..4321)
                     /locus_tag="PLJ11_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03151.1"
                     /translation="MWKVFGEEAVMVQFGPLLKERMIDLSRRKKDRARFLDLIEQVEI
                     VHYLDIHQTLHVFRMDLPRTCTADDVEFLRGFVQRIIKGTEFPMVDLDGEEQKAVLII
                     SAEEPEDEHRVRMAEWYKMKREMDQKREA"
     gene            4406..4996
                     /locus_tag="PLJ11_00330"
     CDS             4406..4996
                     /locus_tag="PLJ11_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOO03152.1"
                     /translation="MQVLDTVQGWRGQASDQLYEWRERSSVATKVCAALFLALLMALA
                     AQVRIVLPFTPVPFTGQVLVVLLGAAVLGRYGALGQGLYLGMGTAFGMFSGMAGAAAL
                     VGPTGGYLIGFMLASCILGELVERREGWTLLHVAGAMALGVLAIYLPGAIQLALVLGL
                     DVGQAVTMGVLPFLVGDAIKVLLASGIGSMFLRPSP"
     gene            5036..5230
                     /locus_tag="PLJ11_00335"
     CDS             5036..5230
                     /locus_tag="PLJ11_00335"
                     /inference="COORDINATES: protein motif:HMM:NF021415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HOO03153.1"
                     /translation="MSLLPEHSHCLFCDAAVEVGQDYCSEECRAKAETEARRERARTI
                     AFLGLAMAVLIAVMLFMALG"
     gene            complement(5214..6371)
                     /gene="folP"
                     /locus_tag="PLJ11_00340"
     CDS             complement(5214..6371)
                     /gene="folP"
                     /locus_tag="PLJ11_00340"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOO03154.1"
                     /translation="MARVRVYRSRAEAGEEWRRLGLPEGALDLSEIDLRRFIFLDSLS
                     PEGMEVLGKELAPHGGTVLPCHGPRALVAIPPLALGALVPAEASVLLRELQDAVRDRY
                     AGEPPAFEWKGGSLDLSRPRVLGILNVTPDSFSDGGRHLDADAAVARAWQMRREGADV
                     IDVGGESTRPRSMPVPPDVEWARIADVIRRVSAEVGAPVSVDTRRPEVARRALQAGAV
                     IVNDVSGLREGMVDVVRREGAAAIVMHMRGEPATMQNDTGYVDVVGDVYAFLERRVRD
                     AVRAGVPRERLAIDPGLGFGKDLAGNLAIMSRLDELRSMGRPVAIGASRKTFLGRIAG
                     GGPEDRLEASLAAAAVACWQGVHLFRVHDVKETVRTLSAVSAMRTGISPAP"
     gene            complement(6378..7478)
                     /locus_tag="PLJ11_00345"
     CDS             complement(6378..7478)
                     /locus_tag="PLJ11_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOO03155.1"
                     /translation="MSEMIEAESLRRVRKELMTCTYCGFCKSVCPTFQMVGWDPSVAR
                     GRMVLAYGLLQKEVPADPSVLEHLYQCTTCKDCERRCPSKIAVVDVVESARRDLVAAG
                     HMLPRHRALVDRVAATGNPYGEGMGVPNTLGKRPRRAKVGYFVGCTAAYRDRALADAT
                     ISVLDKLGEDYTLLDEVCCGSVLQRIGVDEEAVEQLWRRNVDGIRSLGVEKVIFSCAG
                     CYRMFKEEYPKRLDLGFEVQHITEYLAERDLPLRPYKGVVTYHDPCHLGRHCHVYDAP
                     RKVLSMVPEATFKEMARNRETAACCGGGGGVRSAYPELSRGIAGRRVDEASFADMLVT
                     TCPFCVTNLKAGAAGRAAPEIIDLVQLVDRLL"
     gene            complement(7471..8712)
                     /gene="larA"
                     /locus_tag="PLJ11_00350"
     CDS             complement(7471..8712)
                     /gene="larA"
                     /locus_tag="PLJ11_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOO03156.1"
                     /translation="MHLLLPYGEKGTMEADVPATVPVHVVGPCEVSCSPDEIEKALSR
                     PCGGRTLKSFLEGAKDVVFIVNDGTRPTPTAKVLDALVERMDLRRARYLIATGAHRAP
                     TEEELLYIFGDHLEDLRERIVVHDARTSPCVRIGRSSNGTDMEVNEIAVNADRLIIIT
                     SVEPHYFAGYTGGRKSFLPGVASFRTIEQNHRLAMRPEAQTMVLEGNPVHEDMMDALS
                     TVKKDIFSVQVVLDRHHGIYRAAAGDLHLAFKKAVEWADQVFSVTLPKRYDAVITVAP
                     YPMDVDLYQSQKAIDNAKWAVKEGGVIILVSKCREGLGDTTFVTQLCMSRDKRAVLEA
                     MRTGYRLGYHKAAKLADVMCHADLWAVTDLPPQVLEDMGIAPFLSLQEAVDELLRQKA
                     KAEVLVLLDGSVTVPRVRTDE"
     gene            9003..9965
                     /gene="thiL"
                     /locus_tag="PLJ11_00355"
     CDS             9003..9965
                     /gene="thiL"
                     /locus_tag="PLJ11_00355"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOO03157.1"
                     /translation="MARLSDIGEKGIVRSLVSMLTVSARLGPGDDAAAVELGDRYLVA
                     STDMLWEGTHFHPRMSRWQRGWMAAAVNLSDIAAMGAQPLGLLLAMGLPADLDEGDVK
                     GMVQGAADCCDAYGTDYLGGDTKECPQLVLAGASLGLVGKEGLLTRKGASRGDLLMMT
                     GTAGMAAAGLLALELGIDENRAITALFEPQPRVKEGALLSASGVVTSCTDISDGLSTS
                     LQELALASGTAFEVDRPSVPIGPDVERIALKAGKDPMDLALHGGGDYQLLFTIRPDGL
                     DRVLDALGSDARVIGQCIGGNRNVLRSGGVRSELEPKGYEHFRR"
     gene            9946..10968
                     /gene="amrS"
                     /locus_tag="PLJ11_00360"
     CDS             9946..10968
                     /gene="amrS"
                     /locus_tag="PLJ11_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOO03158.1"
                     /translation="MSISDVEPKEAAFWEREDGAVRCLLCPHSCLVREGQKGICRVRG
                     NIGGTLRALSYGKVSSVHLDPMEKKPLFHFHPGEPVLSFGGVGCNLRCLHCQNYSIAQ
                     VGLGDLELMEVPPDEVPGMCVRSGSNGVAWTYNEPTIWYEYMMDASRACKAEGLFTVS
                     VTNGYIQEAPMRALKGVIDAVNIDVKGFTEEFYRQVCKGSLAPVLRTCEVAKEVGMHV
                     ELTYLVIPGRNDRQEEIASFCQWVRDRMGVDTPVHFSRFHPDYRMTDVPPTPRSVMDM
                     AYKEGVAHGLEYVYVGNMATERGESTYCPRCGALLVRRYGFSAEPVGVTDRRCAKCGR
                     ETDIIW"
     gene            complement(10979..11860)
                     /locus_tag="PLJ11_00365"
     CDS             complement(10979..11860)
                     /locus_tag="PLJ11_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03159.1"
                     /translation="MEELTWARIGLAVVVMAIASVSDWRSRTASDTHWYVMGMGGALL
                     FGLRLWEEGAPWPYLACLALIALVFIDTLRDRPGMFEDGVNLPPLLLYALTAIGFGYL
                     TVEHFGEGAYWALVTIPLLMLLFFILYQLDVIKGGADAKALMALSLVFPEYPVLEGLP
                     LLELGEPAALTLMPFPLLVLFNAAILTLLMPLAFLVVNLARGDVRFPVMLFGTRMALE
                     EAEQRHVWSMERVVDGAVRLVLFPRSDEEEGWDALREAGVERPWVTPKVPFLIPLTVA
                     VPFSLLVGNPLLYLMGV"
     gene            11942..13516
                     /locus_tag="PLJ11_00370"
     CDS             11942..13516
                     /locus_tag="PLJ11_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOO03160.1"
                     /translation="MACRGLMVRSRQALFHLTGLMMLGLVVLMGALAPAVSAEPVNAF
                     LIGEEQRETSAGTTAKFEWVLYNNDTFDYLIIPSCVLPSESEYSIEFDQSFIELEPGE
                     SKSLIMAVSTAREMQATTLSFEVVFEIRQLNDGNSTFSLVRSVQLEVTPVFGAMAGSN
                     KVLGIWENNMPWPLDGNAGAFLVSVAIWAVIALFIILVADPLLHRFTSKTATELDDLV
                     LKIVRGPVLLLMVLYGAVSSLEILNLDSGLIADLEMAYSVAFIMVMAWLIYKIYDGVI
                     LYYGHKLATKTDTEVDDVLIPILEKLGMIIIPLVALAVILSLFGYNLTVILAGLGFMG
                     IVIGYAAQATLANFFAGLQMMFDRPFKIGDLLRLDNGDICEVRHIGMRATHLYNTFTH
                     ELIVVPNNDIANKKIVNMVLPDRKLKIAVKVSAAYGSDVDLVMRLMREAAQALPEVLD
                     DPDHQTVVRFTEFADSSLDFTAFIWVKDLDLQFKVASDYRTEVLRRFADNNIEIPFPQ
                     SVVWLKDLSKVERPKV"
     gene            13570..14076
                     /locus_tag="PLJ11_00375"
     CDS             13570..14076
                     /locus_tag="PLJ11_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO03161.1"
                     /translation="MEVKEAIRARRSIRRYKRKELPLEVLDRLLEMARAAPSGANRQA
                     WELVVITDRLRLKGLVPICKEQAFIGDCSAFLVGVEDPHQKWAKVDLAIALDHLSLAA
                     TEEGLGSCWIGAFDPSRLAEYIGLPPDRTITVCMALGYPDEAPAPRGRKKPEELFSWD
                     RYGVRERE"
     gene            14073..15017
                     /locus_tag="PLJ11_00380"
     CDS             14073..15017
                     /locus_tag="PLJ11_00380"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOO03162.1"
                     /translation="MIDREFHLADHDLYLATFKLAAQIPKGMVSTYGAIARALGDIAA
                     SRAVGQILAADMERPFPVPCHRVIYSDGRTGWYMGKGEGAEKKQGLLRSEGVQVVDGR
                     VLDLDRALFTQFSGEPLLATMADSQREIAALVDQEGDALGFQRIAGLDVSYRGDEAFA
                     AMVVVDRRGKVLEERSARCPVRFPYVPGYLGFREMRPYSRVIGEPREDTLYLIDGHGK
                     AHPRRAGIACQLGVVHGVASAGVAKTILTGAMRGSSLLLEGEEVGRLVRTPGGRAYLV
                     SVGHRASLGSVCRLVSSLPSDPLTMAHRLASAERRAEA"
     gene            15014..15268
                     /locus_tag="PLJ11_00385"
     CDS             15014..15268
                     /locus_tag="PLJ11_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03163.1"
                     /translation="MTDEGNGAVPPDDRAGELSNPAPALLISKGSGVEGHEKRWAFPG
                     MTGRPDDRAANRGTERTTPVPTLRAEAMALSKGTHQRHDR"
     gene            15544..15819
                     /locus_tag="PLJ11_00390"
     CDS             15544..15819
                     /locus_tag="PLJ11_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOO03164.1"
                     /translation="MIDRLDDRIMEILKKDSRRPFVEIANELEVSEGTIRSRVKKLLD
                     EGVIQAFTIKTSSKNVKALVEVKIEVNVNTSEIADRIAEFEGVSEVY"
     gene            15835..15963
                     /locus_tag="PLJ11_00395"
                     /pseudo
     CDS             15835..15963
                     /locus_tag="PLJ11_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            15968..16840
                     /gene="dapA"
                     /locus_tag="PLJ11_00400"
     CDS             15968..16840
                     /gene="dapA"
                     /locus_tag="PLJ11_00400"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOO03165.1"
                     /translation="MFKGCATALITPFREDGEIDEEGLRELVRMQERAGVDALVPCGT
                     TGESATLTHKEHLKVIGIVREEAKRAQVIAGAGSNATHEAIHLSKGAKDLGVDGVLLI
                     SPYYNKPNQKGIFRHYEAIAKAVDIPIVVYNVPSRTGSNIAASTVKRLSEVPNIVALK
                     EASGNMAQINAILASVPEGFSVLSGDDLFTYPMMALGAKGVISVTSNVVPELMVEMTH
                     AALEGDWARARALHFKLLPLFNDLFLDTNPIPVKTAMRMLKRPAGVFRLPLCDMEPAT
                     TETLRKTLVAMGLL"
     gene            16858..17637
                     /gene="dapB"
                     /locus_tag="PLJ11_00405"
     CDS             16858..17637
                     /gene="dapB"
                     /locus_tag="PLJ11_00405"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOO03166.1"
                     /translation="MIDVAVAGATGKLGSMVCSLVREQADMRLVGAVVSAEGGKAGTE
                     ICPGVRAVGPDGLEELCHRCTVLVDVTNAAAAERNVPVAIKAGANVVVGTTGLSPVLL
                     ERMGAMVRAQGTSALVTPNFSIGVNVFWKACQTLARALPEYEVEIIEVHHDKKKDAPS
                     GTAVKAAQLIAAENGVDRFVHGRNGMVGARGREIGIHAVRAGDVVGEHTVIFAGNRER
                     IELTHRAHSRMAFAEGCIVAIRWIAPLRDGKVHGMDEVLGL"
     gene            17634..>18345
                     /locus_tag="PLJ11_00410"
     CDS             17634..>18345
                     /locus_tag="PLJ11_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOO03167.1"
                     /translation="MRKVMKFGGTSVGSAEAMARTADIVLSYDCQRAVVVSAMAGVTN
                     SLIEVMGEGRREVQEYLGWLRQRHLDVAMKGVSKELMPDYLEQLEHRLSGLGHLIHLY
                     RQDEDRIMYQDAVSSWGERLSSLTLAFILRSRGADAVALTSEEAGIAATGPPGNGSAV
                     LPATEANLKRTVVPLLREGKVPIITGYYGVDDRGRPLTFGRGGSDYSAAVIGYGVDAD
                     AVEIWTDVDGFMSADPRVV"
BASE COUNT         3391 a         5783 c         6044 g         3127 t
ORIGIN      
        1 tcccaacgca tgggggccgg gggcggtcct tgaagcgagc tggtggcgct ccatcatcac
       61 cacgaggacg aggagcgggc agaccaccgc caggacgatc gcgagcccca ggaacgccat
      121 ccgcacgtcc ccctccggga acatagggcc gtccacaggc accgcgccct ccaggataac
      181 gaacggcccc accgtgacga cgtccccggc ccaattgcca tcctggtcaa cggtgcggat
      241 gtgaaggtag tacgttccgg gggaaagcgg atgggaggtg tacgaactcc cggtctggtc
      301 catcaccagg tccggggtgg cgttcctatc gcggctcagc aggatgctgt acccgaggac
      361 cccgctgccc tcgtccctcg ccccccacca gatgacgctc agggtgctgt caagggaacc
      421 ctccccgatg gtgtgcgagg attggaaccg gtcagggttc ctaggggggt cgagctccat
      481 gggcacccgg gcgttcctga acaggacggt gatgttggcg tcccgcatcg gccaggcccg
      541 tttcacatcc gccaggctgt tcggtgcgtc atagtacatt atgtcccaaa ggagggcgaa
      601 ctgccggtcc accatgtccc cccgggcgct cccgtccgga cggtccagcg ggtccacgcc
      661 atcgtagagg tcccaaagga cgttggccac cgccccctcc acgatcaccc catccatgtc
      721 cccggcgtcc ccatgcccga aggggccgtc ggcgaagacg gtgctctcca gcgagctccc
      781 atccgcccga agggggtccc cctccacggc ccgctcgaag aactcggccc aaccttcggt
      841 gaaggcgaac ccgggggagg actcgctgtc gatgtaatgc gggtccgggc ccgccccgtc
      901 cgggaagctt cctcccctaa gctcaaagtg tacgcaatgc gcatactcat gcagggcgat
      961 atcccgctgc caagctgcac cctcgaaatg ctcgtctgac ggaatatgga tctcgttccc
     1021 atgggaatgg ggccagtacc cctccggcca cacagcggtc acctgggagc gctcccagcc
     1081 agtgagatca tgcagccatt ggccaccgtc ccgtatggcg tcgtagatgg cccaggcccc
     1141 tcgggcctcg tcccgaataa ggtattctag tcccatgccg cggtcggcgg agaactgctg
     1201 agtgctccaa tggtagttcg gcgtcgtgct catgctcccg tccgccaccc ttgcccagcg
     1261 gtcgtcatcg gcctcgatgc gcgccatgta tgtcccggga acgaggccgg tgaaggtcac
     1321 gctcccggaa gcgtccgtat ggccggaccg caccgtatgg aaagcgctcg ttccccccaa
     1381 ccgcagctgg acgaaggcgc ctgcgcaggg ggccgtctcc atcccgtcca aaagctcgta
     1441 gctgaacgac acggtgatgt tgaacaggga ggccccggcc gaagcgaggg tggccaggga
     1501 tggcgccagc tccgagggtc gagcagcgcc ctcggcctcg gtcgtccaca gaggcccatg
     1561 gcctggagca tagcatgcct catcgttctc gaggggaagg acgagccccg ccccgagcaa
     1621 aagggtagct acgaccagga tcgtccatct tctagagggc atcggaccgt caaggcggtt
     1681 acggatttaa ctactcgccc atccaccgtg cggaccggga gatgaagcgc atagcacgca
     1741 tcgattaaat agttggatga tatatccaac tacccaatgg tgaggatgtg aaggctgcgc
     1801 tcatgcgctc ccccggcgtg ctgagcgtcg aggacgtcga agaccccgcc ctccccgagg
     1861 gaggcgttct gctcagggtc aaggcatgtg cggtctgcgg aacggacatc aagatgctgg
     1921 aggccgggca cagggacctc acctatccac gggttccggg gcacgaggtc accggcgaag
     1981 tgctcaatgc aggctcctca ggtctcagaa agggggacct cgtccaggta tggcccgggt
     2041 ccgcctgtaa ggtctgcgct tcctgccgcg cgggcatgga ccatctgtgc cctcaggtca
     2101 ggataatggg gttcaacacc gatgggggca tggccgaact gctggcagtc gacgatccat
     2161 cccgcctcgt ccccatcggg gactcggacc cggtgctgct caccctggct gagccgctgg
     2221 catgctgcat caacgcccag tcgaagctgg gcgtaggcaa tggggacagc gtgctgatac
     2281 tggggggagg gccgatgggc tgtctgaacg ccttcctcgc ccggcacagg ggggccgaca
     2341 ccatcatgct cagcgagccc aggaccgaaa ggctgtggcc ggtgccgaag ggcctcttcg
     2401 atcacgtgtc cgaaccgaag ggggatgatg taagggacat ggtgatgcag gcgaccgccg
     2461 gcaggggcgc ggacgtgatc atcccctgca caccgaacgt gcgcctggac cgcaagatgt
     2521 tcgaactcct ctccccaggg ggccggctgt gcgtgttctc gggcccacgg catgaggact
     2581 cgccggtccc gatcgacgtc agggacgtcc actatcgcga acttatggtc gtgggcagct
     2641 acgggaacgc gagcggtcac tgcagggagg cggtaggact gttgaggaaa ggagcggacc
     2701 tctcctggct cttcaccggg aggtatccgt tggacgaggt ccgccaggcc ttcgcccacg
     2761 cctccgggcg caccgggcag aaggcagtgg tcatattgtg atgaggagcg aatataatga
     2821 tggaggagca gcttagggag ttcggcgcgt ccgtcgacgc gctcataaga ggagagcaca
     2881 tcggaaggca aaggaccagg gacatgttcc gcgaggtcct tctgaacgag cagccggacc
     2941 tgcaacaggg ggcgttcctg gccgcgctca cctccgccgg gcctaccccg gaggagatcg
     3001 cgggcagctg ggaggcgatc ttcgagctgg acacggttaa ggccaggccc gaggtcgaca
     3061 gggtcctcgt ggagaactgc ggcaccggca tggaccgcat caagaccttc aacgtcagca
     3121 ccctggcctc ggtcgtcgcc gcggccggag gggtctatat ggccaagcat ggggcgcgcg
     3181 ctataacctc gaagtgcggg atagtggaca tggcggagac gctgggcgtg gacgtggaat
     3241 gcgacgcgtc tgtggtgagg aggagcgtgg aggtcgccgg catagggctc ttcaacggaa
     3301 tgagcgccaa ggtgcacccc caggcgctgt caaggatcct ttcccagatc aggttcggga
     3361 cgatcctcaa catcgccggg tccctggcca atcccgcctc ccccgaggtg ggggtaaggg
     3421 gcgtctacgc caaggagctg gtccttcccc tggccctgac catgagggag atggggtacc
     3481 agaggggcat cgtggcccac ggcctggacg ccagcgggga gaggggcatg gacgaactct
     3541 ccaccctggg aaggaccatc atcgccgagt tccacgggga tggggagatc gacagctacg
     3601 agatcacccc tcaggccatg gggctccccg tggcccagga gcgggaggtc ctcagcagcc
     3661 tggacaggga gagggaggcg gcgcgcttca tcagggtgct catggggaag gagaaagggg
     3721 cgcgccggga catcgtggcc ttgaacaccg cccccatact gtacctctgt ggcgatgcca
     3781 gctcattaca ggagggcgtc gggaaggcga tggacatcct ggcgagcggc aagggtctgg
     3841 acaagctaag ggactgggtg agggtccaga acaccgggga cccgggggag aagctggagc
     3901 gactggaccg gatggcggag ctggtttagg cctccctctt ctggtccatc tccctcttca
     3961 tcttgtacca ttcggccatg cggacccggt gctcgtcctc cggctcctcg gcggagatga
     4021 tcagcaccgc cttctgctcc tccccgtcca ggtccaccat tggaaattcg gtgcccttga
     4081 tgatccgttg cacgaaccct ctcaggaact ccacgtcgtc cgccgtgcac gtcctcggca
     4141 ggtccatgcg gaacacgtga agggtctggt ggatgtccag gtaatggact atctcgacct
     4201 gctcgatgag gtcgaggaac cgggcgcggt ccttcttccg cctggacaga tctatcatcc
     4261 gctccttcag gagcggtccg aactggacca tgaccgcctc ttcaccgaag accttccaca
     4321 tgacgcccaa aagcccacta ctggcaatta aagcttgccc ctccctggaa atgtttatcg
     4381 ggcaggcctt catatcgcct cagccatgca agtgcttgac acggtgcaag gatggcgggg
     4441 ccaagcctcc gaccaactgt atgagtggag ggagaggtcc agcgttgcca cgaaggtctg
     4501 tgccgccctc ttcctggcgc tgctcatggc attggccgcc caggtgcgca tcgtgctccc
     4561 gttcaccccg gtgcccttca ccggccaggt gctggtcgtc ctcctcggcg cggcggtcct
     4621 gggcaggtac ggcgcgctcg ggcaggggct gtacctcgga atggggaccg cgttcggcat
     4681 gttcagcggg atggccgggg ccgcggccct agtggggccc accggagggt acctgatagg
     4741 gttcatgctc gcctcctgca tcctgggcga gctggtggag aggagggagg gatggaccct
     4801 cctccatgtc gcgggggcca tggccctcgg cgtcctggcg atctacctgc ccggggccat
     4861 acagctggcc ctggtcctcg ggctggatgt gggtcaggcg gtgacgatgg gggtactgcc
     4921 cttcctggtc ggggacgcca tcaaggtgct gctggcctcc ggcatcggct cgatgttcct
     4981 gcggccttcg ccctaaaccc ttatctcccc cctcaacttt gagggtccga tcgtcatgag
     5041 cctcctcccc gagcattccc actgcctctt ctgcgatgcc gccgtcgaag tgggtcagga
     5101 ctactgctcc gaggaatgcc gggccaaggc cgagaccgag gcccggaggg agcgtgccag
     5161 aacgatcgcc ttcctgggac tggcgatggc ggtcctgatc gcggtaatgc tctttatggc
     5221 gctgggctga tccccgtcct catcgcgctg accgcgctca aagtcctgac ggtctccttg
     5281 acgtcatgca ccctgaagag atggacgccc tgccagcacg ccaccgccgc cgcggcgagg
     5341 ctcgcctcca ggcggtcctc gggaccgccc ccggcgatcc tgccgaggaa cgtcttcctg
     5401 gaggccccga tggccaccgg ccttcccatc gagcgcagct cgtccagacg ggacatgatc
     5461 gccaggttgc cggccaggtc cttgccgaag ccgaggccgg ggtcgatggc cagcctctcc
     5521 cttggcaccc ctgcgcgaac ggcgtccctc acccggcgct ccaagaacgc atagacgtcc
     5581 cccaccacgt ccacataccc agtgtcgttc tgcatggtgg ccggctcgcc gcgcatgtgc
     5641 atcactatcg ccgcggcccc ctcccggcgg acgacgtcca ccatgccctc gcgcagcccc
     5701 gacacatcgt tcacgatgac cgccccggcc tgaagcgccc tccgggccac ctccggccgg
     5761 cgggtatcga ccgaaacggg cgcgccgacc tcggcgctga ccctcctgat cacgtcagcg
     5821 atgcgcgccc attccacatc cggagggacc ggcatggacc ggggccgggt ggactccccg
     5881 cccacgtcga tgacgtccgc cccctccctc ctcatctgcc aggccctggc caccgctgca
     5941 tcggcatcca agtgccttcc cccgtccgag aacgagtccg gggtgacgtt caagatcccc
     6001 agcaccctgg gcctggagag gtccagggac ccgcccttcc attcaaaggc aggcggctcc
     6061 cccgcgtagc ggtcccgcac ggcatcctgg agctcacgga ggaggaccga cgcctccgcc
     6121 ggcactagcg ccccaagggc cagagggggg atcgcgacca gggcgcgagg cccatgacaa
     6181 gggagcaccg ttcccccgtg aggggccagc tccttcccaa gcacctccat tccctccggg
     6241 gacagggaat ccaggaagat gaaccgcctg aggtcgatct cggaaaggtc aagggcgccc
     6301 tccggaagcc cgaggcggcg ccactcctcg cccgcctcgg cccggctacg gtagacgcgc
     6361 acccttgcca taggtcctca cagcagtctg tccacaagct ggaccaggtc gattatctcc
     6421 ggggcggccc ttccggcggc cccggccttg aggttggtca cgcagaacgg gcatgtggtc
     6481 accagcatgt cagcgaacga ggcctcgtcc acccttcggc cggcgatccc ccgggaaagc
     6541 tcggggtagg cggagcgcac ccccccgccc ccgccgcagc acgccgccgt ctcccggttc
     6601 cgggccatct ccttgaaggt ggcctcaggg accatggaca gcaccttgcg cggcgcgtcg
     6661 tagacatggc agtgccggcc caggtggcac gggtcatggt aggtcacgac gcccttgtaa
     6721 ggcctcaggg ggaggtccct ctcggccaga tattcggtga tgtgctgcac ctcgaacccc
     6781 aggtccaatc tcttggggta ctcctccttg aacatgcggt agcagccagc gcaggagaag
     6841 atgaccttct ccacgcccag ggagcggatg ccgtccacgt tcctcctcca cagctgctcc
     6901 accgcctcct cgtccacccc gatccgctgc agcacactgc cgcagcatac ctcgtccagg
     6961 agggtgtagt cctcccccag cttgtccagc accgatatcg tggcgtccgc cagggcgcgg
     7021 tcccgatagg cggcggtgca gcccacgaag tacccgacct tggccctcct gggcctcttc
     7081 cccaatgtat ttgggacgcc catcccctcg ccataggggt tgccggtggc ggccaccctg
     7141 tccaccaggg cccggtgcct cggcagcatg tgcccggcgg ccaccaggtc gcggcgggcg
     7201 ctctcgacca cgtccacgac cgcgatcttg gacgggcatc tccgttcgca gtccttgcag
     7261 gtcgtgcatt ggtagaggtg ctccaggacc gacgggtcgg ccgggacctc cttctgcagg
     7321 aggccgtagg ccaacaccat cctccctcgg gcgacgctgg ggtcccagcc caccatctgg
     7381 aacgtggggc acacgctctt gcagaacccg cagtaggtgc aggtcatgag ttccttccga
     7441 acccggcgga ggctctccgc ctcgatcatc tcactcatcg gtacgcaccc tcggcacggt
     7501 cacgctgccg tcgaggagaa caaggacctc cgctttggcc ttttgcctca gcagttcgtc
     7561 aaccgcctcc tgcagggaga ggaagggggc gatgcccata tcctcaagga cctgcggcgg
     7621 aaggtccgtc accgcccaga ggtcggcatg gcacatcacg tcggccagct tggcggcctt
     7681 gtgatacccc aggcggtacc ccgttctcat ggcctccaac accgcccgct tgtccctgga
     7741 catgcagagc tgggtgacga acgtggtgtc tcccaggccc tccctgcact tggacaccag
     7801 gatgatgacg cccccctcct tcaccgccca cttggcattg tctatggcct tctgcgactg
     7861 gtacaggtcc acgtccatgg gatagggggc gacggtgatg accgcgtcat agcgcttcgg
     7921 gagcgtgacg gagaagacct ggtccgccca ttccacagcc ttcttgaagg ccagatgcag
     7981 gtcgcccgcg gccgcccggt agatgccgtg atggcggtca agcaccactt ggacggagaa
     8041 aatgtccttc ttcactgtgg agagcgcgtc catcatgtcc tcatggacgg ggttcccttc
     8101 cagcaccatg gtctgggcct ccggccgcat ggcaaggcga tggttctgct cgatcgtgcg
     8161 gaaggaggcg acgccaggaa ggaacgattt ccgccccccc gtgtacccgg cgaaatagtg
     8221 cggctcgacg ctggtgatga tgatgagacg gtcagcgttg accgcgatct cgttcacctc
     8281 catgtccgtg ccgttgctcg atctgcctat gcgaacgcag ggggaggtgc gcgcatcatg
     8341 cacgacgatg cgctcccgca ggtcctccag gtggtccccg aatatatata ggagctcctc
     8401 ctccgtcggc gcgcggtgcg ctccagtggc gatgaggtaa cgggcccgcc tgaggtccat
     8461 ccgctccacc agcgcgtcca ggaccttggc ggtgggggtg ggccgcgtac catcgttgac
     8521 gatgaacacc acgtccttgg cgccttccag aaaggacttg agggtacgcc ctccgcaagg
     8581 acgggagagg gccttctcta tctcatccgg ggagcagcta acctcgcagg gaccgacgac
     8641 atggaccggg acggtggctg ggacatccgc ctccatcgtc cccttctcgc catagggtag
     8701 gagcaggtgc atgcgttcca ccgttggcta acgcactatt aaaggattat cacatgcacc
     8761 gatcggtccg ccgtccgcca atgggaagcc cgcctacgga ccctatgcag gtccccgcgg
     8821 ttcgatactt ggggccgatc ggtcataatg gtgctcgaaa cacgtccctt cccctccgac
     8881 cccgtcgcag gcgcgccccg gtcctcatgg acccccatga caagcgttgg cagagggggc
     8941 gaccgagccc tcccgccctt tccatggaaa gggttattcg cccctcttcc cctctcctct
     9001 ccatggcccg gctgagcgac ataggcgaga aggggatcgt gcgctcccta gtatccatgc
     9061 tgaccgtttc ggccaggctc gggccggggg acgacgccgc ggcggtggag ctgggcgatc
     9121 gctatctggt ggccagcacc gatatgctct gggaggggac ccacttccac ccccgcatgt
     9181 cccggtggca gcggggatgg atggccgcgg ccgtcaacct cagcgacatc gcggccatgg
     9241 gcgcccagcc cctgggactg ttactggcca tggggctccc agcggacctg gatgaaggag
     9301 atgtaaaagg gatggtccag ggggcggcgg actgctgcga tgcgtacggc acggactatc
     9361 tgggcgggga caccaaggag tgcccccagc tcgtccttgc cggggcctcg ctggggctgg
     9421 tggggaagga ggggctgctc acgcgcaagg gggcgtcccg gggggacctc ctgatgatga
     9481 cggggacggc cggcatggcc gccgccggcc tgctggcgtt ggagctaggg atcgacgaga
     9541 acagagcgat cacggccctt ttcgaacccc agcctcgagt gaaggagggg gcgctgctct
     9601 ccgccagcgg ggtggtgaca tcatgtacgg acatctctga cggcctttcc acgtccttgc
     9661 aggagctggc cctggcaagc ggaacggcct tcgaggtgga ccgcccctcg gtgccgatcg
     9721 gcccagacgt ggagcggatc gccctgaagg ctggcaagga ccccatggac ctggcgcttc
     9781 acggcggggg cgactatcaa ttgctcttca ccatccggcc ggatggcctg gacagggtgc
     9841 tcgatgcgct ggggagcgat gcgagggtca tcggccagtg catcggaggg aaccggaacg
     9901 tcttgaggag cgggggcgtc cgctcggaac tggagccgaa gggatatgag catttcagac
     9961 gttgagccaa aggaggccgc tttctgggaa agggaggatg gggcggtccg gtgcctcctc
    10021 tgcccccatt catgtctggt ccgggagggg cagaagggga tatgccgggt gcggggcaac
    10081 atcggcggga ccctgcgggc attgagctac gggaaggtct cctccgtcca cctggacccg
    10141 atggagaaga aacccttgtt ccatttccat ccaggggagc cggtcctctc gttcgggggc
    10201 gtgggctgca acctgcggtg cctgcactgc cagaactatt ccatagccca ggtcggcctg
    10261 ggagacctcg agctgatgga ggtacctccg gacgaggtgc ccgggatgtg cgtgcgctcc
    10321 ggctccaatg gcgtcgcctg gacctacaac gagccgacca tctggtacga gtacatgatg
    10381 gatgccagcc gtgcctgcaa ggccgaggga ctgttcaccg tctcggtcac caacggctac
    10441 atccaagagg ccccgatgcg cgccttgaaa ggggtcatcg acgccgtgaa catagacgtc
    10501 aaggggttca cggaggagtt ctacaggcag gtgtgcaaag gatcgctggc ccccgttctc
    10561 cgaacgtgcg aggtggcgaa ggaggtcgga atgcacgtcg agctgacgta cctggtgatc
    10621 cccgggagga acgaccggca ggaggagatc gcctccttct gccagtgggt gcgggaccgg
    10681 atgggggtgg acacgcccgt ccacttctcc cggttccatc cggactacag gatgaccgac
    10741 gttccgccga ccccccgttc cgtcatggac atggcgtaca aggagggcgt ggcccatggg
    10801 ctggagtacg tctacgtggg caacatggcc acggaaaggg gggagagcac ctactgtcca
    10861 aggtgcgggg ccctgctcgt ccgccggtac gggttctccg ccgagccggt cggcgtgacg
    10921 gaccgaaggt gcgccaagtg cgggcgggag acggacatca tatggtgagc gggggccgtc
    10981 atacgcccat cagatagagg aggggattgc ccaccaggag ggagaagggc accgccaccg
    11041 tgagcgggat caggaacggg accttggggg tgacccaggg gcgctccacc ccggcctcgc
    11101 ggagggcatc ccacccctcc tcctcgtcgg agcgggggaa caggaccagc ctcaccgccc
    11161 catcgaccac cctctccatt gaccagacat gcctctgctc cgcctcttct agcgccatgc
    11221 gggtcccgaa aagcatcacc gggaagcgga cgtcccccct tgcaaggttg accaccagga
    11281 aggccagggg catcaacaag gtgagtatgg ccgcattgaa cagcacgagc agcgggaagg
    11341 gcatcagcgt cagggcggcc ggttcaccga gttccaatag cggcagccct tccagtaccg
    11401 ggtactcagg gaacaccagg gacagcgcca tcagggcctt ggcgtccgcc ccgcccttga
    11461 tcacgtccaa ctggtaaagg atgaagaaga ggagcatgag cagagggatg gtgacgaggg
    11521 cccaataggc tccctccccg aagtgctcga cggtcagata gccgaagccg atcgcagtga
    11581 gcgcgtacag cagcaatggg ggcaggttca ccccgtcctc gaacatgccc gggcgatcac
    11641 gtagcgtgtc gatgaagacc agggcgatga gggcgaggca ggccagatat ggccatggag
    11701 caccctcctc ccagagccgg aggccgaaga gcagcgcccc gcccatgccc atcacgtacc
    11761 agtgcgtgtc cgaggcggtc cggctgcgcc agtcggacac cgacgcgatg gccatgacca
    11821 ccacggccag cccgatccgg gcccaggtca gctcttccat gcccggttca tgcacgaatt
    11881 acgattaatt gctttgccat ggctcagcaa gaagcaagaa atctaatata ggatggaggt
    11941 ggtggcctgc cggggactaa tggtacggtc taggcaggcg ctgttccatc tgaccgggct
    12001 catgatgctc ggcctcgtcg tgctcatggg agccctggct ccagccgttt cggccgaacc
    12061 ggtgaacgcc ttcctaatag gggaagagca aagggaaacg tcggcaggca cgaccgccaa
    12121 gttcgaatgg gtcctgtaca acaacgacac ctttgattac ctcatcatcc catcctgcgt
    12181 tcttccaagc gagagtgagt actcgatcga gttcgaccaa tccttcattg agcttgaacc
    12241 aggggaatcc aagtccttga tcatggccgt ctcgaccgcc cgggagatgc aggcgaccac
    12301 gctctccttc gaggtcgtgt tcgagatacg ccagttgaac gatgggaatt ccaccttctc
    12361 gctcgtgcgg tcggtgcagc tggaggtcac tccggtgttc ggggcaatgg cggggagcaa
    12421 caaggtcctg ggcatctggg agaacaatat gccctggccc ctggacggca acgccggggc
    12481 gttcctggta agcgtggcca tatgggcggt gatagccctg ttcatcatcc tggtcgccga
    12541 ccccctcctg cacaggttca cctccaagac cgccaccgag ctggacgacc tggtcctcaa
    12601 gatagtgcgc ggtcctgtcc tcctgctgat ggtcttgtac ggggcggtga gctccctgga
    12661 gatcctgaac ctcgacagtg ggctgatcgc ggacctggag atggcctaca gtgtggcctt
    12721 catcatggtg atggcgtggc tcatctacaa gatatacgac ggcgtgatcc tctactatgg
    12781 ccataagctc gccaccaaga ccgatacgga agtggacgac gtcctcattc ccatccttga
    12841 aaagttgggc atgatcatca tccccctcgt agccctcgcg gtcattctga gcctgttcgg
    12901 ctataacctg acggtgatcc tggccggcct gggcttcatg gggatcgtca tcggatacgc
    12961 ggcccaggcc accctggcca acttcttcgc cgggctacag atgatgttcg atcgtccctt
    13021 caagataggc gacctgctga ggctcgacaa cggggacata tgcgaggtgc gccatatagg
    13081 gatgagggcc acccatctgt acaacacctt cactcatgag ctcatagtgg tccctaacaa
    13141 cgatatcgcc aacaagaaga tagtcaacat ggtcctccct gacaggaagc tgaagatcgc
    13201 tgtgaaggtg tcagcggcct acggcagcga tgtggacctg gtcatgcggt tgatgaggga
    13261 ggccgcccag gccctgcctg aggtcttgga cgatccggac caccagacgg tcgtccgctt
    13321 caccgaattc gccgattcct ccctcgactt caccgcattc atatgggtca aggacctgga
    13381 cctgcagttc aaggtggcct ccgactaccg caccgaggtc ctgagaaggt tcgcggacaa
    13441 caatatcgag ataccgttcc cacagtcagt ggtctggctc aaggacctga gcaaggtcga
    13501 gcgaccaaag gtctgacgaa aaggaataat gccatccaag cgcatttacg atcaacggag
    13561 ctgagcagca tggaggtaaa ggaggccatc agggccagga gaagcattcg caggtacaag
    13621 cgcaaggaac tgcccttgga ggtattggac cgcctcctcg agatggcgag ggccgccccc
    13681 tcaggggcga acaggcaggc gtgggagctc gtggtcatta ccgacaggct aaggctgaag
    13741 gggctggtgc cgatatgcaa ggagcaggcc ttcatcgggg actgctccgc gttcctggtg
    13801 ggggtagagg accctcacca gaaatgggca aaggtggacc tggcaatagc ccttgaccac
    13861 ctttcattgg ccgccaccga ggaggggctg ggttcctgct ggataggggc gttcgaccca
    13921 tccaggttgg ccgagtacat cggactcccc ccggaccgca cgatcaccgt ctgcatggcc
    13981 ctgggctatc ccgatgaggc ccccgccccc cggggtagga agaagccgga ggagctcttc
    14041 agctgggacc gctacggggt ccgggaaagg gaatgatcga ccgggagttc catctggccg
    14101 accacgacct ctatcttgca acgttcaagc tggccgccca gatcccgaag gggatggtca
    14161 gcacctatgg ggccatcgcc agggccctgg gcgacatcgc cgcctcccgc gcggtcggcc
    14221 agatcctggc cgcggacatg gagcgcccgt tcccggtgcc atgtcatagg gtcatctact
    14281 ccgatgggcg caccggctgg tacatgggga agggcgaggg ggcggaaaag aagcaggggc
    14341 tgctgcgctc cgaaggcgtg caggtggtcg acggccgggt gctcgacctg gaccgtgcgc
    14401 tcttcaccca gttctcgggc gagccgctat tggcgaccat ggccgattcc cagagggaga
    14461 tcgcggccct ggtcgaccag gagggcgacg ccctcggctt ccaacgcata gcgggcctgg
    14521 acgtttcata tcggggggac gaggccttcg ccgccatggt cgtggtcgat cggcgcggga
    14581 aggtcctgga ggaacggtcg gcccgctgcc ccgttcgctt tccatacgtg cccggctacc
    14641 tggggttccg ggagatgagg ccttattccc gtgtcatcgg ggagccccgg gaggacacgc
    14701 tgtatctcat cgatggtcat ggcaaggccc atccccggag ggcgggcatc gcctgtcagc
    14761 tcggggtcgt gcacggggtg gcctcggcag gggtggcgaa gaccatcctg acaggggcga
    14821 tgaggggtag ctccctcttg ctggaggggg aggaggtcgg tcgtctggtg aggacgccgg
    14881 gaggaagggc gtacctcgtt tccgtgggcc atcgtgccag cctgggctcg gtgtgccgac
    14941 tggtctcctc cctgccctcg gacccattga ccatggcgca ccggctggcc agcgcggaga
    15001 ggagggccga ggcatgaccg atgaaggaaa cggggcggtt ccccccgatg atagggcagg
    15061 ggagctttcc aaccccgccc cggcgctcct catatcgaag ggatcgggag tagagggcca
    15121 tgaaaagagg tgggctttcc cgggcatgac agggcgcccc gatgaccgcg cagccaaccg
    15181 gggaacggag cgcacgaccc ccgtcccgac cctccgagcc gaggccatgg cattgagcaa
    15241 gggcacgcat cagcggcatg atcgatgaga tggagcaccg atactgcatg atgcatctgg
    15301 accgctttag gcagggggcg ggccccgggc caaaagaccg gaggaccgcg gtcgcgagga
    15361 aaggcgttca tgcccgaagg ctcgttcggg aagcggatca ggtccccatc gaagcgggat
    15421 gcgccctccg caggccgtat cctttcaata tttacgcgct ttctaggaat atattaataa
    15481 tattttacta ttaactgagg tttgcatacg aaatcggaaa aaatcacctg ggaggatgtt
    15541 agattgatcg accggttgga cgacaggata atggagatac tgaagaagga ctcacgacgt
    15601 cccttcgtgg agatagccaa tgagctagag gtttccgaag ggaccatccg gtcccgggtg
    15661 aagaaactgc tcgacgaggg agtgatccag gccttcacga taaagaccag cagcaagaac
    15721 gtcaaggcat tggtggaggt gaagatcgag gtgaacgtca acacctcaga gattgcggac
    15781 cggatcgccg agttcgaggg cgtttctgag gtctactagg tcaccgggga agaggacata
    15841 gtggtaataa tcgacgtcac ctcgtcccct cagctgaacg agatcatcga gcgcatccgt
    15901 cggttcgaca acgtggaatc gaccaggacg cggctgatac tcaaggagca tttcggaggt
    15961 tgatactatg ttcaagggct gcgccaccgc gctgataacc ccgttccggg aggatgggga
    16021 gatagatgag gaaggattgc gagagctggt gcgcatgcag gagagggcag gcgtggacgc
    16081 cctcgtcccg tgcggcacga cgggcgagtc cgcgaccttg acgcacaagg agcacctcaa
    16141 ggtcatcggc atcgtgcggg aggaggccaa gagggcccag gtcatcgccg gcgccggcag
    16201 caatgccacc cacgaggcca tccacctctc caaaggggcc aaggacctgg gcgtggacgg
    16261 cgtcctgctc atctccccct actacaataa gccgaatcag aagggcatct tccgccatta
    16321 cgaggcgatc gccaaggcgg tggacatccc catcgtggtg tacaacgtcc cgagccgcac
    16381 cggttcaaac atagcggcca gcacggtcaa gcggctctcc gaggtgccca acatagtggc
    16441 gctgaaggag gccagtggca acatggccca gatcaacgct atccttgcca gtgtccccga
    16501 gggcttcagt gtgctgtccg gggacgacct gttcacctat ccaatgatgg ccctgggcgc
    16561 caagggtgtg atctccgtga cctccaacgt cgtcccggag ctgatggtgg agatgaccca
    16621 cgctgccctc gaaggggact gggcaagggc cagggcattg cacttcaagc tgctcccgct
    16681 cttcaacgac ctgttcctgg atacaaatcc catcccggtg aagacggcca tgcggatgct
    16741 caagcggccg gcgggcgtct tccgtctgcc gctatgcgac atggagccgg ccacgactga
    16801 gaccctcagg aagaccctgg tggccatggg gctgctttaa ggcagggggg ctcaggcatg
    16861 atcgacgtgg cggtggccgg ggccacaggc aagctaggct ccatggtgtg ttcccttgtc
    16921 agggaacagg ccgatatgag gctggtcgga gcggtcgtat ccgccgaggg agggaaggct
    16981 ggcacggaaa tatgcccagg cgtcagggcc gtgggcccgg acggattgga ggagctgtgc
    17041 catcgttgca cggtgctggt cgacgtgacc aacgccgccg cggccgagag gaacgtcccg
    17101 gtggcgatca aggccggggc taacgtcgtg gtcgggacca cgggcctctc gcccgttctc
    17161 ctcgaacgga tgggcgcgat ggtcagggcc cagggcacgt ccgcattggt cacccccaac
    17221 ttctcgatcg gggtgaacgt cttctggaag gcctgccaga ccttggcaag ggcattgccg
    17281 gagtacgagg tggagatcat agaggtccac catgacaaga agaaggacgc gccgtcggga
    17341 acggcggtca aggccgctca gctcattgcc gcggagaacg gggtggaccg cttcgtgcat
    17401 gggcggaacg gaatggtcgg tgcccgagga agggagatag gcattcacgc tgtccgggcc
    17461 ggggacgtgg tcggggagca cacggtcata ttcgcgggca accgcgagcg catcgagctg
    17521 acacaccggg cgcactcgcg catggccttt gccgagggct gcatagtcgc catccgatgg
    17581 atagccccct tgagggacgg caaggtacat gggatggacg aggtgctcgg tctatgagga
    17641 aggtcatgaa gttcgggggg accagcgtag gctccgccga ggcgatggcc cgcaccgcgg
    17701 acatcgtgct gagctacgat tgtcagaggg ccgtcgtcgt ttcggccatg gccggggtga
    17761 ccaactccct catcgaggtc atgggcgagg ggagaaggga ggtccaggag tacctgggct
    17821 ggctcaggca gaggcacctc gatgtggcca tgaaaggggt gtccaaggag ctgatgcccg
    17881 actaccttga gcagttggag catcggctgt ccggtctggg gcacctcatc catctgtaca
    17941 ggcaggacga ggaccggatc atgtaccagg acgcggtctc cagctggggg gagcgcctct
    18001 cctccctcac cctggccttc attcttcgta gccggggggc ggatgcggtg gcactcacct
    18061 cggaggaggc gggcattgcg gccaccggcc cccctggcaa cggaagtgcg gtcctaccag
    18121 cgaccgaagc gaacttgaag cgaacggtgg taccgctgtt gagggagggc aaggttccga
    18181 tcataacagg ctattacggc gtggacgacc ggggccggcc attgacgttc ggccgcggcg
    18241 gctccgatta ctcagcggcc gtgataggat acggggtgga cgccgacgcg gtggagatat
    18301 ggaccgacgt ggacggcttc atgtccgccg acccccgggt ggtga
//
LOCUS       DAOSYN010000005        18256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2534_length_18256_cov_4.596945, whole genome shotgun sequence.
ACCESSION   DAOSYN010000005 DAOSYN010000000
VERSION     DAOSYN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 18256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18256
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2534_length_18256_cov_4.596945"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..148
                     /locus_tag="PLJ11_00415"
     CDS             41..148
                     /locus_tag="PLJ11_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03168.1"
                     /translation="MAYADRLNPGLLAPGGMPGRLTVFTESALRKIGEW"
     gene            151..429
                     /locus_tag="PLJ11_00420"
     CDS             151..429
                     /locus_tag="PLJ11_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOO03169.1"
                     /translation="MTRKVLLYPYVTEKTMNYMTGTPTQEFKDGNKIEFIVDRSATKA
                     EIKEAFEAHFEVKVDKVWTKVSKEGKHAVIKLAEGYSAEDIGMRIGVF"
     gene            441..1139
                     /locus_tag="PLJ11_00425"
     CDS             441..1139
                     /locus_tag="PLJ11_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOO03170.1"
                     /translation="MGRQLRQQRRGRGSSTYRSPSHRHVGEIRHPSAELKAGKVMDIV
                     HAPGRNCPVAEVRFENGAREKVLAAEGLMVGQEVRVDGEVSLKPGSIMPLANIPEGTL
                     VHNLEARPGDGGKFVRTAGTYATVITKGDTVVVQLPSGALKNFDPRCRAGVGIVAGSG
                     ASEKPFAKAGKKYHAYRSKSKAYFKVKGVAMNAIDHPHGGGGHPHVGKPSTVSRNAPP
                     GRKVGRLSPQKKRK"
     gene            1146..1607
                     /locus_tag="PLJ11_00430"
     CDS             1146..1607
                     /locus_tag="PLJ11_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOO03171.1"
                     /translation="MADEKLTGTKASRRKQRKKKGMMTARRKKEFTYRGFTLEQLQEM
                     PFEEMVDLLPSRVRRSFRRGLNEEQKATFDKIMAGEQEVVRTHRRDIPVVPAFVGRRV
                     AVHNGKEFKEIEVKPEMIGHFLGEFAMTRSPVKHSGPGVGATRSSKFMPLK"
     gene            1619..2065
                     /locus_tag="PLJ11_00435"
     CDS             1619..2065
                     /locus_tag="PLJ11_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOO03172.1"
                     /translation="MVGYTAQADPDTTSRAIAKELPISPKFSREICRMVRGKKVDDAI
                     RMLEEVVELRRPVPIRRYNYGVAHKPGIGPGRYPTKAAAAIIKVLESAKSNAEYKGLD
                     TDNMRVRVISAHLGRTIPGYMPRAHGRSTAWDQQTVNIEVILEEAQ"
     gene            2066..2782
                     /locus_tag="PLJ11_00440"
     CDS             2066..2782
                     /locus_tag="PLJ11_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOO03173.1"
                     /translation="MASERKFVAENVRRVLLKEYLMKTVDRAGFGGVDVQRTPMGTRV
                     TLVTERPGIVIGRRGAAIKSLTKAIEDNFKYDNPQIEVQEVENPNLNAQIMAEKLAFA
                     LERGWHFRRAGHSTVRRIMEAGARGCLIVISGKLTGERHRTEKFKDGYIKYCGEPREL
                     FVDTGFAVAKLKPGVIGVKVEIMHPEARLPAEIDVMDMDAALQANPDLADLLRKPEEA
                     SVIVEHPAEDTSVKDEEVTD"
     gene            2783..2983
                     /gene="rpmC"
                     /locus_tag="PLJ11_00445"
     CDS             2783..2983
                     /gene="rpmC"
                     /locus_tag="PLJ11_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOO03174.1"
                     /translation="MALIRTSEIRQMSADERAAKLKELQDELMHERGAAAMGGAPNNP
                     GKIRALRSNIARILTVQKEEEQ"
     gene            2983..3282
                     /gene="yciH"
                     /locus_tag="PLJ11_00450"
     CDS             2983..3282
                     /gene="yciH"
                     /locus_tag="PLJ11_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015492826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HOO03175.1"
                     /translation="MADICSVCGLPKELCMCEEIAREQQSVRIYTDSRRYGKIVTVVD
                     GIDATDIDIDDLARKLKNKCAAGGTAKEGKIELQGDHKKKVAQTLEEMGFKTEVR"
     gene            3306..3548
                     /locus_tag="PLJ11_00455"
     CDS             3306..3548
                     /locus_tag="PLJ11_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein subunit"
                     /protein_id="HOO03176.1"
                     /translation="MRQEFIGLDIEVVESSCPGHLVRGKVVDETKNMLAISCQGRERW
                     IPKSGHEFVFTYQGERVRVLGSEIQHRPEDRIKRIR"
     gene            3553..3879
                     /locus_tag="PLJ11_00460"
     CDS             3553..3879
                     /locus_tag="PLJ11_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOO03177.1"
                     /translation="MSEIRDIGIDVKAPSATCEDKHCPFHGQLPVRGQIIDGVVVSVK
                     MNGSAVVERNYLKYDKKYERFEKRMKRYTAHNPPCLGIAAGDRVRIMECRPLSKTVSF
                     VVVEKR"
     gene            3884..4282
                     /gene="rpl14p"
                     /locus_tag="PLJ11_00465"
     CDS             3884..4282
                     /gene="rpl14p"
                     /locus_tag="PLJ11_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOO03178.1"
                     /translation="MKGLAGNQTRGIITGSVLDVVDNTGAKTISVIAVPSYHGVHGRC
                     PAAAVGDIVIASVKKGTPEMRRQVVYAIIVRQRRPFRRADGTMVHFEDNAAVITTEDG
                     ETKGSDIKGPVAREAAERWPRVAATASTIV"
     gene            4292..4663
                     /gene="rplX"
                     /locus_tag="PLJ11_00470"
     CDS             4292..4663
                     /gene="rplX"
                     /locus_tag="PLJ11_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOO03179.1"
                     /translation="MTSKYARKQRKALYNAPNHVRTKGVGSHLSDELIEKLGRRTVRV
                     VKGDTVKVVRGDEDVRGLEGKVTRVDTQSGRLIIEGVTIAKADGTMTERPVHASNVII
                     TKLDMKDSYRKQKLQYEEGSQ"
     gene            4660..5370
                     /locus_tag="PLJ11_00475"
     CDS             4660..5370
                     /locus_tag="PLJ11_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HOO03180.1"
                     /translation="MTQDLKRLNAPKSWPVPRKSNVWVTSPNPGPHPLERCMPLSVVL
                     RDLLKVADTAAEAKKILSAKRVLVDGKVVTEEKFPVGLMDVVSLVPSGKHYRALLDNR
                     GRFILVEIGEDRAKWKLCRIENKTYVAGGRLQLNLHDGRNILVDTDSYKTGDVLKIEL
                     PSQRILDAYKLVQGNMAMIISGSNAGQINVVEEQVEERSTAPNLVRFKNGILTIKDNV
                     FVIGNKTPEILLPEVRAA"
     gene            5367..5882
                     /locus_tag="PLJ11_00480"
     CDS             5367..5882
                     /locus_tag="PLJ11_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOO03181.1"
                     /translation="MSENVMRRPYLEKVVVNMGVGEAGERLVKAQKVIEMVTGQRSRQ
                     TFSKVTNRDFGIREGMPIGCLVTLRGERAEEFLRRAFSIRENRIPSYSFDREGNLSFG
                     IADYTDFSGMKYDPEIGIFGMDISVSIQRPGRRVARRRIMRRSIPRSHRMTREEAMEF
                     IAQKFNVEVID"
     gene            5879..6025
                     /locus_tag="PLJ11_00485"
     CDS             5879..6025
                     /locus_tag="PLJ11_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOO03182.1"
                     /translation="MKPKKKFGRSVGCTRCGRRRGIVRRYGMHLCRQCFREIAPQIGF
                     KKYS"
     gene            6035..6424
                     /locus_tag="PLJ11_00490"
     CDS             6035..6424
                     /locus_tag="PLJ11_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOO03183.1"
                     /translation="MQSDPLNDAMSTIKNAAAVGKSECVIRPSSKLIGRVLKVMQDNG
                     YINQFEFVEDGKAGMFKVMLEGRINNCGVIKPRYSVKKVDMEEYEARYLPAQDFGVLI
                     LTTTDGVMTHMEAKEKGVGGKLLAFVY"
     gene            6435..6983
                     /locus_tag="PLJ11_00495"
     CDS             6435..6983
                     /locus_tag="PLJ11_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOO03184.1"
                     /translation="MTVTGHLDERVHVPKEVKVAMKGAEVTVKGPKGTVTRDFSHPRV
                     SVAFKDDTFHVECEYPRTKEKALVGTYAAHLRNMIEGANHGFEYALKMVYSHFPMKIM
                     VKGDRFVIENFLGEKAPRSAPILSGAKVTIKGNEVLVTGSDLEKVSQTAANIERATRI
                     KGYDPRVFQDGIYIVEKARRPK"
     gene            6983..7627
                     /locus_tag="PLJ11_00500"
     CDS             6983..7627
                     /locus_tag="PLJ11_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HOO03185.1"
                     /translation="MSAKKEIRRIQDLPYYREEYTAQLAEMGIEETEELLAALNDEDR
                     KKEIVDALDGVGNKIADHWIELLSEEGTEIVEEEAKTVPKVKPVLDAELKAALELRGA
                     KNAARPAFLRQEWFRYQRLGEKWRKPKGIHSKMRRHLSYRPPVVSIGYRGPKAVRGYH
                     PSGFQEVLVHTVGQLDDVNPKVQAIRIGGTVGGRKRAAITERADELGIRVLNRT"
     gene            7633..8088
                     /locus_tag="PLJ11_00505"
     CDS             7633..8088
                     /locus_tag="PLJ11_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015492815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HOO03186.1"
                     /translation="MDLKNQKRMAAEVLKCGENRVWIDPNRIEDVADAITRADIRTAI
                     QSGSIRALPVRGVSRGRARHRSAQKAKGRRRGQGSRKGTSTARRPKKEAWIQTIRPLR
                     ATLRGLRDEGKISPKVYREFYMKAKGGMFRSRNNLLMHLKTEGYLKEDN"
     gene            8088..8588
                     /locus_tag="PLJ11_00510"
     CDS             8088..8588
                     /locus_tag="PLJ11_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOO03187.1"
                     /translation="MAQGPRYKVAFRRRREGRTDYRQRARLLRAGVPRAVVRTSLRHI
                     SVQIVDFDPQGDRVLVTAHSRELLGMGWDKATGNVPAAYLTGYLAGKKAVAKGVNEAV
                     LDIGLKVPAKGATAFAALKGMLDAGMRIPHGKEVLPSKERLAGKHIGDDVERMVMDIK
                     TRMEAN"
     gene            8590..9267
                     /locus_tag="PLJ11_00515"
     CDS             8590..9267
                     /locus_tag="PLJ11_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOO03188.1"
                     /translation="MDWVPKTRLGKMVLNGEITTMSQALATKLPLREPEIVDILLPDL
                     KDEVIDVNIVQRMTDSGRRVKFRITCVVGNGDGFIGIGRAKGKEVGPSIRKAIDNAKL
                     NIIEIKRGCGSWECGCGAPHSLPFTVVGKSGSVEVVLKPAPRGISLAVGDVAKSLLTL
                     AGVKDSWGFAKGHTKTTVNYAYATFYALQNTIEMRVSEEQERRQHIVSGPVQVHVAEE
                     GAPGQEE"
     gene            9270..9734
                     /locus_tag="PLJ11_00520"
     CDS             9270..9734
                     /locus_tag="PLJ11_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOO03189.1"
                     /translation="MAYAAVRIRGHSGVRSDVEDTMRMLRLTRANHCVVLPETDEIKG
                     MLMKAKDYITWGEVSEETLARMIKFRGRLVGDRPIDDQVVKDNTPFGSIMAFAKGIVK
                     GEAKYSDMKDVKPLFRLSPPKKGYEGVKRGFGAGGALGYRGEEINDLISRMI"
     gene            9746..10174
                     /locus_tag="PLJ11_00525"
     CDS             9746..10174
                     /locus_tag="PLJ11_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HOO03190.1"
                     /translation="MVSRTEKFRGSRTHGRGKKSGRGAGKHGGKGNAGLHKHKAQYML
                     KYMPDHFGRHGFKRPQKTVSAKITMNVGDLEQTLQVMKAQGTAVERDGKISIDLGALG
                     VDKLLGNGQVSTPFVVSVAEATALAKAKVEKAGGHIVVPE"
     gene            10191..11771
                     /gene="secY"
                     /locus_tag="PLJ11_00530"
     CDS             10191..11771
                     /gene="secY"
                     /locus_tag="PLJ11_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOO03191.1"
                     /translation="MADDQKSRLYKLKPITDRLPAVSKPEGHVHFRTKMLWVIVILVF
                     YFVMTNVFLYGLDSEKTVDLFAQYRAILAGAQGSLMHLGIGPIVTASIIMQLFVGAKI
                     IKLDLTNREDKAVYQSTQKFLVIVMILVEAVPQVFGYLVPSDSLISGLNGTFGASGLL
                     RGENIARLIIVVQLCVGSYLVFLMDEVVSKWGIGSGISLFIAAGVAEALFTGTLNTEG
                     YYPDQPLSNSNLPVGTIPKTIYILTHQSAADLASGGYEQILIANPNPLIALIGTIVIF
                     LFVAYVESARIELPLAHGTARGARGRYPIKLIYASNIPVILMAALLANVSMFTMLFYT
                     NDFLSGIPLIGHADWLGVYPPGENRATSGLAWYLSTPSGVGDWLLPMLDPGTYGSMVY
                     GHSSLKVLLHVLIYVGVMVGGSVLFAKFWIMTTNMGPEAVARQIESSGLQIPGFRRDP
                     RVLKRVLERYIPVVTVISGAVVGALAAGADLIGTVGNASGTGVLLAVGIMIQFYEALG
                     REQMMEMHPVLRGFFGGE"
     gene            11778..12431
                     /locus_tag="PLJ11_00535"
     CDS             11778..12431
                     /locus_tag="PLJ11_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HOO03192.1"
                     /translation="MADAPPQNAKATTSRMLMIFVFVMAIFVMFDQNLRTSLGELVGL
                     GLTPLWGFDGTMPVVTLFLTGAFMTFLSIVVRHFFTDYVKQAESQRIMSAFNKEMRQA
                     RLENNTYKLKKLMEMQPKVMEESMKQTTTQMKLMPVTMLIIIPIFAWLAVFVGNMDSA
                     LITVPWSKVADLNDTYVLPSWVLLYSLISIPFGQVLSRGLRYFSFKRRLKELKAEGK"
     gene            12428..12958
                     /locus_tag="PLJ11_00540"
     CDS             12428..12958
                     /locus_tag="PLJ11_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO03193.1"
                     /translation="MRITISGTIGSGKTTVCRLLSEALGWPTAISGHLFREMAKELGM
                     SLEEFGRLAEQDPKYDRLIDDRLLQRARQEEDVILEGRLAGQLLRRNAIPALCVYLDA
                     PLEVRAKRVSGREGVGLREAMDEMSAREASEALRYRRFYDIDVTDRSIYDMVIDTGAL
                     TPEQVVERIIARVRGG"
     gene            12960..13916
                     /locus_tag="PLJ11_00545"
     CDS             12960..13916
                     /locus_tag="PLJ11_00545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HOO03194.1"
                     /translation="MLVRDKGPRTLARGKRPEDRTVEELLDGGVIVLDKVQGPTSHQV
                     TAWVKDILGVERIGHGGTLDPNVSGVLPIALGRAVRMTDLVLRSDKQYVCHMYLHRPV
                     PEEKVREVIGTFVGPIYQTPPVRSAVKVAMRVRKVSSIDVLEVDGRDVLFKVDCDAGT
                     YVRTLCVDIGEALGVGANMRALRRTRSGAMTEKEAVTLQELTDAVVHWREGDDTWLRR
                     IIRPMEVLLEPLPKVIVKDSAVDAVAHGADLNAPGIVSLDEGIEKGAQVALLTLKGEA
                     LALGTATMAARDMHRAYKGRAVRTVRVFLPEGTYPRMWGAQQ"
     gene            complement(13907..14920)
                     /locus_tag="PLJ11_00550"
     CDS             complement(13907..14920)
                     /locus_tag="PLJ11_00550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO03195.1"
                     /translation="MERDGPSVRLGNGIEVAYGDTTFFLDPRKVTAEGVCCVSHAHSD
                     HMPSSFAGGPIHCSDITLRCMRGRVKSKRSKNYLEDPERNNSPLVEASGAGHILGSNM
                     FMLDAGQRVLYTGDLCPQSRLGIEGARPQKCDVLIIEATYGDPRYRFPDRREMIRVIH
                     DWVEDMLDQGYSAVLHAYPLGKSQELITMLRDLRPRLHGSVLETTRMMEADDLRFDYL
                     PFDPEDLDPQLVISPGAWGENGLSVLRKKRSADVSGWCLGTGGRSGGRMGWGMDQGFA
                     YSDHADFYELLDFIDRCDPSVVYTHHGSEGTLAQEVQGRLGITAIPLTREKKGQTNLG
                     RYC"
     gene            15297..16034
                     /locus_tag="PLJ11_00555"
     CDS             15297..16034
                     /locus_tag="PLJ11_00555"
                     /inference="COORDINATES: protein motif:HMM:NF020014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03196.1"
                     /translation="MYRSMPETVTEPLVRALHPEPDGMLATTAIPPVDELLGGLAPGT
                     VTTIDSSDRMLFALVNMLSVNFVREWHRDVIWVDGGNSVNPYDLTYICKRFRLRPEEV
                     LNSISVSRAFTAYQMSTLVEESLEEELVRSRGGMVIVSCYPDLFQDADMDWDESLKLM
                     ERGMGKLRSLTSKHRTVSVVTNYGLAKLMYRKGLRSLFYEKADRVVSVEHRSGYVRIS
                     LPREEREIMYRPVPPNQTTLDEYRRRF"
     gene            16034..16273
                     /locus_tag="PLJ11_00560"
     CDS             16034..16273
                     /locus_tag="PLJ11_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03197.1"
                     /translation="MGRTVPTYRMASESLFQSWSDFRRALPQEDRAAFDRLSDKVRMH
                     ASACTVAAFLDPLEGALLSILLEQEKELERLRKKA"
     gene            16278..>18256
                     /locus_tag="PLJ11_00565"
     CDS             16278..>18256
                     /locus_tag="PLJ11_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HOO03198.1"
                     /translation="MRGWILDCYADEVNNEMVTWVKTPAGVERIADREYRPSFYVKPS
                     AERKQDLLLGLKEAGVEHRWERHRTWLTGPLEDVLKVTPKGYRNLMPLAKTVDRWGCH
                     RDHTLFNVDMRMDTRYFLYRKVFPMGLMDVERWHNLDAPFRLDYPMPELRTCELTLET
                     SARRGMPTLEDRLKRATLDDTVMEGKEADILDMLQATLRAKDPDVLVTDGGDSFHLPF
                     LERCAARNEMELELGREGGKRLGKGKSYFTYGRILYKPPAYKLRGRIHLDKGSSFIYQ
                     EGGMAGLIDLCRLARISMQDMARMSPGSAISAMQVNEASKMGSLVMWKKNLPEGFKTA
                     AELVACDRGGLIYEPKVGLHERVQEVDFTSLYPSIMVRHNISPETLNCECCSDGGEPV
                     PGLGYWTCTKRQGLLGRVLEPLVRRRIALKRLKREGGCRSAIYDQRAKVLKWVLVTCF
                     GYTGYRNARFGRIECHEAITAYGREILLRSAEVAEHRGFRILHGIVDSLWLEGNGDVE
                     AYCREIGQRMGIPLVWDGEYKWIVFLPNLTNGAGALNRYYGAFQDGELKVRGIALRRR
                     DTCTLARELQQGMLDRLALGEDAEGFRHRIPEALDVLDTFVRNLRDMETPLEKLVLSK
                     RITRELSEYRQRNDQFRALCQLEDRGFRTPPGEAV"
BASE COUNT         3890 a         5228 c         6136 g         3002 t
ORIGIN      
        1 cgcggtggtc aagggcgcca ggaacctacc aggcgtggaa gtggcctatg cggataggct
       61 gaaccctggc ctgctggcgc cgggcgggat gccgggaagg ttgacggtgt tcaccgagtc
      121 cgccctcagg aaaatagggg agtggtaagc atgaccagaa aggtactgct ttacccgtac
      181 gtgacggaaa agaccatgaa ttacatgacc ggcacgccca cccaggagtt caaggacggt
      241 aacaagatcg agttcatcgt ggaccgctcc gccaccaagg cggagatcaa ggaggccttc
      301 gaggcgcatt tcgaggtcaa ggtggacaag gtctggacca aggtctccaa ggagggcaag
      361 cacgctgtga tcaagctcgc tgaaggatac tcggccgagg atatcggcat gaggatagga
      421 gtgttctgag gagggggaac atgggaagac aactgagaca acagaggagg gggcgcggat
      481 cctccaccta taggtccccg agccacaggc acgtgggaga gatcaggcac cctagcgccg
      541 aactcaaggc cggcaaggtg atggacatag tgcacgctcc aggccggaac tgcccggtgg
      601 ccgaggttag gttcgagaac ggggcaaggg agaaggtcct cgcggccgag gggctgatgg
      661 tcggacagga ggtccgtgtg gatggcgagg tgagcctgaa gccgggcagc atcatgcccc
      721 tggccaacat tccagagggc accctggtcc acaaccttga ggcccggccg ggcgatggcg
      781 gtaagttcgt taggaccgct ggcacctacg ccaccgtgat caccaagggc gataccgtgg
      841 tcgtgcagct gccctcaggc gcgctcaaga acttcgatcc ccgctgccgg gcgggcgtgg
      901 ggatcgttgc ggggagcgga gcatccgaga agccctttgc caaggcgggg aagaagtacc
      961 acgcctaccg cagcaagagc aaggcgtact tcaaagtgaa gggggtggcg atgaacgcca
     1021 tcgaccatcc gcatggcggt ggcggccatc cgcacgtcgg caagcctagc accgtatcga
     1081 ggaacgctcc gccgggaagg aaggttggta ggctttcccc ccagaagaag aggaagtgat
     1141 tctagatggc tgacgagaag ctaactggca ctaaggcctc gaggaggaag cagaggaaga
     1201 agaagggcat gatgaccgcc cggaggaaga aggagttcac ctaccggggc tttaccctgg
     1261 agcagctcca ggagatgccc ttcgaggaaa tggtggacct ccttccatct agggtgagaa
     1321 ggtccttccg tcgcggtctc aacgaggagc agaaggccac gttcgacaag ataatggccg
     1381 gggagcagga ggttgtgcgc acacaccgcc gggacatacc cgtcgtgcct gcgttcgtgg
     1441 gccgccgggt cgcggtgcac aacgggaagg agttcaagga gatcgaggtg aagcccgaga
     1501 tgatcgggca tttcctgggg gagttcgcca tgaccaggag cccggtgaag cactccgggc
     1561 caggcgtcgg tgcgaccagg tcctccaagt tcatgccgct gaagtgaggt gacgatacat
     1621 ggtaggatac accgctcaag ccgacccgga tacgacgtcc agggccatcg ctaaggagct
     1681 gcccatctcc cccaagttct cccgtgagat ctgcaggatg gtgagaggca agaaggtcga
     1741 tgatgccatc cgcatgctgg aggaggtcgt ggagctgcgc cggccggtcc cgatccgccg
     1801 ctacaactac ggggtggcgc acaagcccgg catagggccg ggccgatacc cgacgaaggc
     1861 cgccgcggcc atcatcaagg tgctggagag cgccaagagc aacgcggagt ataagggcct
     1921 tgataccgac aatatgcgcg tgcgcgtgat ctcagcccat ctgggcagga ccatccccgg
     1981 gtacatgccg agggcgcacg gtcgcagcac cgcctgggac cagcagaccg tcaacatcga
     2041 ggtcatacta gaggaggcac aataaatggc cagcgagagg aagttcgttg cggagaacgt
     2101 ccgacgggtt ctcctcaagg agtacctgat gaagaccgtc gaccgcgctg gcttcggagg
     2161 cgtggacgtg caaaggaccc ctatggggac cagggtcacc ctggtcaccg agcgcccggg
     2221 catcgtgatc ggtcgccgcg gggcggccat caagtccctg acgaaggcga tcgaggacaa
     2281 tttcaagtac gataaccccc agatcgaggt ccaggaggtg gagaacccca acctcaacgc
     2341 ccagatcatg gcggagaagc tggccttcgc cctagagcgc ggctggcact tcaggcgggc
     2401 ggggcattcc accgtccggc gcataatgga ggcgggcgcg cggggctgcc tcatcgtcat
     2461 ctccggcaag ctgaccgggg agaggcaccg caccgagaag ttcaaggacg gttacataaa
     2521 gtactgcggc gagccgaggg agctgttcgt ggacacagga ttcgccgtgg ccaaactgaa
     2581 gcccggagtg atcggggtca aggtcgagat catgcaccct gaggccaggc ttccggccga
     2641 gatcgacgtc atggacatgg atgccgcatt gcaggctaac cccgacctcg cggacctgtt
     2701 gcggaagccg gaggaggcgt cggtcatcgt ggagcatccc gcggaggata cctccgtgaa
     2761 ggacgaggag gtgaccgact aaatggcatt gatcaggacc tccgagataa ggcagatgag
     2821 cgcggatgag cgcgccgcca agctcaagga gctgcaggac gagctcatgc acgagcgggg
     2881 ggcggccgcc atgggcggcg ctcccaacaa cccggggaag atcagggcct taaggagcaa
     2941 catcgccagg atcctaaccg tccagaagga ggaagaacaa taatggccga catttgctcg
     3001 gtatgtgggc tccccaaaga gctgtgcatg tgcgaggaga tcgcaagaga gcagcagtcc
     3061 gtaaggatct acacagacag caggcggtac ggcaagatcg tcactgtggt cgatgggatc
     3121 gatgccacag acatagacat cgacgacctg gcacgcaaac tgaagaacaa gtgcgccgcc
     3181 gggggcaccg ccaaagaggg taagatcgaa ctccaggggg accacaagaa aaaggtggcc
     3241 cagaccctgg aagaaatggg cttcaagacc gaggttcggt aggagccatg gacaagaggg
     3301 atttcatgag gcaagaattc ataggactgg acatcgaggt ggtggaatcc agctgccccg
     3361 ggcacctcgt gcgcgggaag gtggtggacg agaccaagaa catgctggcc atctcctgcc
     3421 agggcaggga gaggtggata cccaagtccg gccatgagtt cgtgttcaca taccagggcg
     3481 agcgggtcag ggtcctagga tccgagatcc agcatcggcc agaggatagg atcaagagaa
     3541 ttaggtgatc taatgagcga aatcagagac attggcattg atgtcaaggc cccctcggcc
     3601 acctgcgagg acaagcactg tccattccac gggcagctcc cggtgcgggg gcagatcata
     3661 gacggcgtcg tcgtatccgt caagatgaac ggcagcgccg tggtcgagag gaactacctg
     3721 aagtatgaca agaagtacga acgcttcgag aagaggatga aacggtacac cgcccacaac
     3781 cccccgtgcc tcggaatagc ggccggggac cgggtgcgca tcatggaatg tcgccccctg
     3841 agcaagacgg tgtccttcgt cgtcgtggag aagaggtgag gccatgaagg gactggcagg
     3901 aaaccagacg cggggcatca taaccggctc cgtcctggac gtggtggaca acaccggtgc
     3961 caagaccatc agcgtcatcg cggttcccag ctaccacggc gtccacgggc gctgccccgc
     4021 cgcggccgtg ggcgacatag tgatcgcgtc ggtcaagaag ggcaccccgg agatgaggag
     4081 acaggtggtc tacgcgatca tcgtccgcca gaggaggccc ttccgccgcg cggacggcac
     4141 catggtgcac ttcgaggaca acgccgcggt catcaccact gaggacgggg agaccaaggg
     4201 gagcgatata aagggaccgg tggccaggga ggcggcggag cgctggcctc gggtggcggc
     4261 caccgcatcg accatcgtat gaggtgagga aatgacgagc aagtatgcga ggaagcagag
     4321 aaaggcgctt tacaacgccc ccaaccatgt acgcaccaag ggcgtcggct cccatctcag
     4381 cgatgagctg atcgagaagc taggacgccg caccgtgcgc gtggtgaagg gggacaccgt
     4441 caaggtggtc cgtggggacg aggacgtccg cggcctggag ggcaaggtca cccgcgtgga
     4501 tactcagagc gggagattga taatcgaggg cgtgacgatc gccaaggccg atggcacgat
     4561 gaccgagagg cccgtgcacg cgtccaacgt gataatcacc aagctggaca tgaaggacag
     4621 ctacaggaag cagaagctgc aatacgagga gggatcccag tgacccagga tctgaagaga
     4681 ttgaacgcac cgaagagctg gccggtgccc aggaagagca atgtgtgggt gaccagcccc
     4741 aaccctggcc cgcatccgct tgagaggtgc atgccgttga gtgtggtgct ccgggacctc
     4801 ctcaaggtag cggacaccgc cgccgaggcc aagaagatcc tgtcagcgaa gcgggtgctg
     4861 gtagacggga aggtcgtaac cgaggagaag ttcccggtgg gcctgatgga cgtcgtcagc
     4921 cttgtcccgt ccggcaagca ctaccgtgcg ctcttggaca atcggggcag gttcatcctt
     4981 gtagagatag gcgaggaccg cgccaaatgg aagctctgcc gcatcgagaa caagacgtat
     5041 gtggccgggg gccgcctgca gctcaacctg cacgacgggc gcaacatcct ggtcgatacg
     5101 gacagttaca agaccggcga tgtgctcaag atagagctcc ccagccaaag gatactggac
     5161 gcctacaagc tggtgcaggg taacatggcc atgatcatca gcggctcgaa cgctggccag
     5221 atcaacgtgg tggaggaaca ggtagaggag cgctccaccg cccccaacct ggtccgtttc
     5281 aagaacggca tccttaccat caaggacaac gtgttcgtca tcggcaataa gaccccggag
     5341 atcctccttc cggaggtgag ggcggcatga gtgagaacgt gatgcgccga ccgtacctcg
     5401 agaaggtggt cgtgaacatg ggcgttgggg aggcgggcga gcgcctcgtc aaggcgcaga
     5461 aggtcatcga gatggtcacc ggacagaggt ccagacagac cttctccaag gtgaccaacc
     5521 gcgacttcgg catccgcgag gggatgccca tcggatgcct tgtgaccctg cggggagaaa
     5581 gggcggagga gttcctcagg agggcgttca gcatccgcga gaaccgcatc ccctcctatt
     5641 ccttcgacag ggaggggaac ctctccttcg gcatagcgga ctacactgac ttctccggga
     5701 tgaagtacga ccctgagatc ggcatattcg gaatggacat cagcgtgtcc atccagaggc
     5761 ctggaagacg tgtggccaga cgccgcatca tgcgccgctc catccctcgg tcccaccgca
     5821 tgaccaggga ggaggcgatg gagttcatcg cacagaagtt caacgtcgag gtgatcgatt
     5881 gaagcccaag aagaagttcg gaaggagcgt gggctgcacc aggtgtggcc ggagaagggg
     5941 catagtccgc cggtacggca tgcatctctg ccggcaatgc ttcagggaga tcgcgccgca
     6001 gatagggttc aagaagtact cgtgaggtga aaagatgcag agcgatccat tgaatgatgc
     6061 gatgtccacg atcaagaacg ctgcggccgt agggaagagc gagtgcgtga tcaggccctc
     6121 ctccaagctt atcggccggg tgctcaaggt gatgcaggac aacggctaca tcaaccagtt
     6181 cgagttcgta gaggacggga aggccggaat gttcaaggtc atgctggaag ggaggatcaa
     6241 caattgcggt gtgatcaagc cgcgctattc ggtgaagaag gtggacatgg aggagtacga
     6301 ggcccgctat ctgccggccc aggacttcgg tgtgctcatc ctgaccacta ccgatggcgt
     6361 gatgacccac atggaggcca aggagaaggg cgtgggcggg aagctgctcg cgttcgttta
     6421 ctgaggggaa tgacatgaca gtaaccggac atttggacga gagggtgcac gtgccgaagg
     6481 aggtgaaggt cgccatgaag ggggccgagg tcaccgtcaa ggggcccaag ggcacggtaa
     6541 ccagggactt cagccacccc cgggtgagcg tcgccttcaa ggacgacacc ttccatgtgg
     6601 agtgcgagta ccctcgcacc aaggagaagg ccctcgttgg cacgtacgcg gcccatctga
     6661 ggaacatgat cgagggggcc aaccacggct tcgagtacgc cttgaagatg gtctactccc
     6721 acttccccat gaagatcatg gtgaagggtg ataggttcgt catcgagaac ttcctgggcg
     6781 agaaggcgcc acgctccgca cccatcctga gcggggccaa ggtcaccatc aaggggaacg
     6841 aggtcctcgt caccggctcc gacctggaga aggtctcgca gaccgccgcc aacatcgagc
     6901 gcgccacccg gatcaagggc tatgacccgc gggtgttcca ggacgggata tatatagtag
     6961 agaaggcaag gaggcccaag tgatgtcggc caagaaggag atcaggagga tccaggacct
     7021 cccctattac cgggaggagt acaccgctca gctggccgag atgggcatcg aggagacgga
     7081 agaactcctg gcagcgttga acgacgagga ccgcaagaag gagatcgtcg atgccctgga
     7141 cggcgtaggc aacaagatcg ccgaccattg gatagaactg ctcagcgagg aggggacgga
     7201 gatcgtggaa gaggaagcga agaccgtccc gaaggtcaag cccgtgctcg atgccgagct
     7261 caaggccgcc ctggagctga ggggcgccaa gaacgcagcc cgcccggcct tcctgaggca
     7321 ggagtggttc cgctaccaga ggttgggcga gaagtggagg aagcccaagg gcatccactc
     7381 caagatgcgg cgccatctct cgtaccgccc gccggtcgtc tcgatcggct accgcggacc
     7441 gaaggcggtg cgcggctatc acccatccgg gttccaggag gtcctggtac ataccgtagg
     7501 gcagctggac gatgtgaacc ccaaggtgca ggccatccgc atcggcggca ccgtgggcgg
     7561 aaggaagaga gcggcgataa ctgagagggc ggatgaacta ggcatccgcg tattgaacag
     7621 gacgtgagac agatggatct taagaaccag aagcgcatgg cggccgaggt cctgaagtgc
     7681 ggggagaaca gggtctggat cgatccgaac cgcatagagg acgtcgcaga cgccataacc
     7741 cgggcggaca tccgaacggc catccagtcc gggtccatcc gcgccttgcc cgtcagggga
     7801 gtgtccagag gacgggcccg tcaccggtcg gcccagaagg ccaagggacg caggcgtgga
     7861 cagggcagca gaaagggcac ctccacggcc aggaggccga agaaggaagc ctggatccaa
     7921 acgatccggc ccctgcgcgc cacgctgcgt gggctgcggg acgagggcaa gatcagcccg
     7981 aaggtgtacc gtgaattcta tatgaaggcc aagggcggca tgttcaggag caggaacaac
     8041 ctgctcatgc acttgaagac cgaaggctac ctcaaggagg ataactgatg gcccagggac
     8101 cgagatacaa agtggccttc cgccgcaggc gcgaaggccg gaccgattac agacagaggg
     8161 cgaggctgtt gagggccggg gtcccgcgcg cggtcgtgcg cacctccctg cgccatatca
     8221 gcgtgcagat cgtggacttc gatccccagg gggaccgggt cctagtcacc gcccactccc
     8281 gcgagctgct gggaatggga tgggacaagg ccaccggcaa cgttcccgcc gcatacctca
     8341 ccgggtacct ggccgggaag aaggccgtcg ccaagggagt gaatgaggcc gtactcgaca
     8401 tcggcctgaa ggtgcccgcc aagggcgcca cggcgttcgc cgcgctgaaa ggcatgctgg
     8461 acgccggaat gcgcatcccc cacggcaagg aggtgctgcc gagcaaggag cggcttgcgg
     8521 gcaagcacat cggcgatgac gtggagagga tggtaatgga catcaagacc cgcatggagg
     8581 cgaactgaga tggactgggt acctaagacc aggttgggaa agatggtgct caatggtgag
     8641 ataaccacca tgagccaggc actggcaacc aagctccccc tgcgcgagcc ggagatagtg
     8701 gacatactgc tccccgacct caaggacgag gtcatcgacg tcaacattgt gcagaggatg
     8761 accgactccg ggagaagggt gaagttccgc atcacctgcg tggtaggcaa cggcgatggc
     8821 ttcatcggca tcggtcgggc gaaggggaag gaggtgggac cctccatccg caaggcgata
     8881 gacaacgcca agctgaacat aatcgagatc aagcggggat gcggctcgtg ggagtgcggg
     8941 tgcggcgccc cgcactcgct tcccttcacc gtcgtcggca agtccggttc ggtggaggtc
     9001 gtccttaagc ccgccccgag ggggatatcc ctggcggtgg gggacgtggc caagagcctc
     9061 cttacgctcg ccggggtcaa ggactcctgg ggcttcgcca aggggcatac caagacgacc
     9121 gtgaactacg cgtatgcgac cttctacgcc ctgcagaaca ccatcgagat gcgcgtctcg
     9181 gaggagcagg agaggaggca gcacatcgtc agcgggccgg tgcaggtgca cgtggccgag
     9241 gaaggcgccc ccggacagga ggaatgagca tggcatatgc agccgtacgc atccgcgggc
     9301 attccggcgt ccgtagcgac gttgaggaca ccatgcgtat gcttcgcctc acccgggcga
     9361 accattgcgt ggtccttccc gagacggatg agataaaggg catgctgatg aaggccaagg
     9421 actacatcac ctggggagag gtctcggagg agacgctggc ccgaatgatc aagttccgcg
     9481 gccgattggt tggcgacagg ccgatagacg atcaggtggt caaggacaat accccgttcg
     9541 gttccataat ggccttcgcc aagggcatag tcaagggcga ggcaaagtac tcggacatga
     9601 aggatgtgaa gcccctgttc cgcctgagcc cgcccaagaa gggctatgaa ggggtcaagc
     9661 gcggcttcgg ggccgggggt gccctgggct accggggaga ggagataaac gacctcatct
     9721 ccaggatgat ctaaaggtga tatcgatggt tagcagaacc gagaagttca ggggctccag
     9781 gacccatggt cgcggaaaga agagcggtcg aggtgccgga aagcacgggg gcaagggcaa
     9841 cgctggcctg cacaagcaca aggcccagta catgctgaag tatatgcccg accatttcgg
     9901 ccggcacggc ttcaagcggc ctcagaagac cgtgtccgcg aagatcacga tgaacgtggg
     9961 cgatctggag cagaccctgc aggtcatgaa ggcccagggg accgcggtcg agagggacgg
    10021 aaagatctcc atcgaccttg gcgccctggg cgtcgacaag ctgctgggga acggccaggt
    10081 gagcacgccc ttcgtggtct ccgtcgcgga ggccaccgcc ttggcgaagg ccaaggttga
    10141 aaaggcaggc gggcatatcg tggtgccaga gtaagtagca cgtggtgctc atggccgacg
    10201 accagaagag caggctgtac aagctgaagc cgataacgga ccgcctaccg gcggtttcca
    10261 aacccgaagg gcacgtccat ttccggacca agatgctgtg ggtcatagtc atcctggtgt
    10321 tctacttcgt tatgaccaac gtcttccttt acgggttgga cagcgagaag accgtggacc
    10381 tgttcgccca gtacagggcc atcctggccg gggcgcaggg ctcgctcatg catctgggca
    10441 taggcccgat agtcaccgcc tccatcatca tgcagctgtt cgtgggagcg aagataatca
    10501 agctcgatct caccaaccgc gaggacaagg cggtctatca gagcacccag aagttcctgg
    10561 tcatagtgat gatcctggtc gaggcggtgc cccaggtgtt cgggtacctg gtcccttccg
    10621 attccctcat ctccgggctc aacgggacgt tcggggcctc ggggctcctc cggggagaga
    10681 acatcgcccg attgatcatc gtcgtccaac tatgcgtcgg ctcatatctc gtgttcctca
    10741 tggacgaggt ggtgtcgaag tggggtatag gcagcggcat ctcactgttc atcgccgccg
    10801 gcgtcgcgga ggcgctgttc accgggacgc tgaacaccga gggctactat ccggatcagc
    10861 cattgagcaa tagcaatctt ccagtaggaa cgatccccaa gacgatctac atcctgaccc
    10921 atcagtcggc ggccgacctt gccagcggag gctatgaaca gatcctcatc gcaaacccca
    10981 atccccttat cgcgctcata ggcacgatcg tcatcttcct cttcgtggcc tatgtggaat
    11041 cggcccgcat cgaactgccc ctggcgcatg ggaccgcgag gggggcgcgg ggaagatacc
    11101 cgatcaaact gatctacgcg tccaacatcc cggtcatcct gatggccgcg ctgctggcga
    11161 acgtcagcat gttcaccatg ctgttctata ccaacgactt cctgagcggc atccctctca
    11221 tcggacatgc agattggttg ggggtctatc cccccggaga gaaccgggcc acctccggtt
    11281 tggcctggta cctctccacg cccagcgggg taggggactg gctcctgccg atgttggacc
    11341 cgggcaccta cgggagcatg gtctatggac atagctccct gaaggtgctg ctgcacgtgc
    11401 tcatctacgt gggcgtgatg gtcggaggct ccgtcctatt cgccaagttc tggatcatga
    11461 ccaccaatat ggggccggag gccgttgcaa ggcagatcga gtcctcgggg cttcagatcc
    11521 ctggcttccg gcgcgacccc cgggtgctca agcgggtgct ggaaaggtac atccctgtcg
    11581 tgacggtgat ctccggggcc gttgtcgggg cattggcggc cggagcggat ctgatcggga
    11641 cggtggggaa cgcgagcggg accggcgtgc tgctggcggt gggtataatg atccagttct
    11701 acgaggcgct gggtcgcgag cagatgatgg agatgcaccc cgtgctgcgg ggattcttcg
    11761 gtggagagtg atgaggaatg gcagacgcac cgccccagaa cgccaaggcg accaccagca
    11821 gaatgctgat gatcttcgtc ttcgtcatgg cgatcttcgt gatgttcgat cagaacctga
    11881 ggaccagcct cggggagctg gtcgggctag gcctgacccc tctctggggc ttcgatggga
    11941 ccatgcccgt tgtcaccttg ttcctcacgg gggcgttcat gaccttcctc agcatagtgg
    12001 tccgccactt cttcaccgat tacgtgaagc aggctgaaag ccaacggata atgtccgcct
    12061 tcaacaagga gatgcgccag gcccgcctgg agaacaacac ctacaagctg aagaaactga
    12121 tggagatgca gccgaaggtc atggaggagt ccatgaagca gaccaccacc cagatgaagc
    12181 tgatgccggt gaccatgctc atcatcatcc ccatcttcgc ctggctggcg gtcttcgtcg
    12241 gcaacatgga cagtgcgctc atcacggtcc cgtggtccaa agtggccgac ctgaacgaca
    12301 cctatgtgct cccatcgtgg gtgctgctct attcgctgat aagcatccca ttcgggcagg
    12361 tgctttcaag ggggctgagg tacttctcgt tcaagaggcg gttgaaagag ctcaaggccg
    12421 aagggaaatg aggatcacca tcagcggaac gatcggcagt ggcaagacca cggtgtgccg
    12481 gctgctcagc gaggccctgg gatggccgac ggccatatcc gggcacctct tccgggagat
    12541 ggccaaagag ctgggcatgt ccttggagga gttcggcagg ctggccgagc aggaccccaa
    12601 gtacgacagg ctcatcgacg accgcctcct ccagcgcgca cggcaggagg aggacgtcat
    12661 cctcgagggg agattggcag ggcagttgct tcgccgcaac gccatcccgg ccctctgcgt
    12721 ctatctggac gcacccctgg aggtacgcgc caagcgcgtc tccgggcggg agggggtagg
    12781 gctgcgggag gccatggacg agatgagcgc ccgtgaggcg agcgaagcgc tgaggtaccg
    12841 caggttctat gacatcgatg tgaccgaccg ttcgatctac gacatggtca tcgacactgg
    12901 cgccctcacc ccggagcagg tggtcgaacg gatcattgcc cgcgtcaggg gcggttgaga
    12961 tgctcgtccg ggacaagggg ccgaggacgc ttgcccgggg gaagcggccc gaggaccgga
    13021 cggtggagga gctcttggat gggggggtca tcgtcctcga caaggtgcag gggccgacct
    13081 cgcatcaggt caccgcctgg gtgaaggaca tcctgggggt ggagaggatc ggccacggag
    13141 gaacgctcga ccccaatgtc agcggggtgc tccccatcgc cctggggagg gcggtgcgca
    13201 tgaccgacct cgtcctccgc tcagataagc agtacgtgtg ccacatgtac cttcatcggc
    13261 cggtcccgga ggaaaaggta cgggaggtca tcgggacgtt cgttggcccc atataccaga
    13321 ccccgcccgt gcgctcggcg gtcaaggtgg ccatgagggt acggaaggtc agctcgatcg
    13381 acgtcctgga ggtggacggc agggacgtcc tgttcaaggt ggactgcgat gccgggacgt
    13441 acgtccgcac gctgtgtgtg gatatcgggg aggcccttgg ggtgggggcg aacatgaggg
    13501 ccctccggcg cacccgctcg ggcgcgatga ccgagaagga ggcggtcacg ctgcaggagc
    13561 tcacggacgc tgtggtgcac tggagggaag gggatgatac ctggctgcgc cgcatcatca
    13621 ggccgatgga ggtccttttg gagccgctgc cgaaggtcat cgtcaaggac agcgcggtcg
    13681 atgcggtggc gcatggggcg gacctgaacg cccccggcat cgtctccctg gacgagggca
    13741 tcgagaaggg ggcgcaggtc gccctcctga ccctgaaggg ggaggcgctg gcgctgggca
    13801 ccgccaccat ggccgcgcgg gacatgcaca gggcatacaa gggaagggcg gtccggaccg
    13861 tgcgggtgtt cctgcccgaa gggacgtacc cccggatgtg gggcgctcag cagtagcggc
    13921 ccaggttcgt ctgccccttc ttttcccgcg tgagcgggat ggccgtgatg cccagccgcc
    13981 cctggacctc ctgggcgagc gttccctccg agccatggtg ggtgtagacc acggacgggt
    14041 cgcacctgtc tatgaaatcg aggagctcat agaagtccgc atggtccgag tacgcaaacc
    14101 cctgatccat gccccatccc atcctgcccc cggaccggcc tccggtcccg aggcaccacc
    14161 cggagacgtc ggcggagcgc ttcttcctga ggacactgag gccgttttcc ccccaagccc
    14221 ccgggctgat gaccagctgg gggtcgaggt cttcgggatc gaagggaagg taatcgaagc
    14281 gcaggtcgtc cgcctccatc atgcgggtgg tctcgagcac cgagccgtgc aggcgcggcc
    14341 ggagatccct cagcatggtg atgagttcct gggatttgcc gaggggatag gcgtgcagga
    14401 ccgccgaata gccctggtcc aacatgtcct cgacccagtc gtggatgacc ctgatcatct
    14461 cccggcggtc cgggaagcgg taccttgggt cgccgtaggt ggcctcgatt atcagcacgt
    14521 cgcacttctg cggtctggca ccctctatgc ccaggcggga ctgggggcag aggtccccgg
    14581 tgtacaggac cctctgcccg gcatcgagca tgaacatgtt cgacccgagg atgtgcccgg
    14641 cgccgcttgc ctcgaccagg gggctattgt tccgttccgg gtcctccagg tagttcttgg
    14701 accgcttgct cttcaccctc ccgcgcatgc accgcagggt gatgtccgag cagtggattg
    14761 ggcccccggc aaagctggag ggcatgtggt cggaatgggc gtgggagacg caacagacgc
    14821 cctctgcggt gaccttacgt gggtccagga agaaagtggt gtcgccatag gcgacctcga
    14881 tgccattgcc taagcgaacg gaaggcccat ccctttccat cgggcggaga atgcatggtc
    14941 ggctatttac cgatagcgcg cgggcctttg atcgtggcac cgtcccttcg gaccctgcca
    15001 tgccatcaat aggcgagcat ggccatgacc aagacgatca ttatcacctg cgccgtgcct
    15061 aggacgcgct cgaccattcg cttgcgtcga agctcccatt cgtgtatcga catgctcatt
    15121 ttgctcaccg atatttatta cgccctgtaa gatatagata ttgcctcggg gtcctggccc
    15181 caaaggacga tcgacggcac acgcacaacg accctttgag ctgtccccgc ccattttcac
    15241 ccacctcccc ctgtcagccc tttataccta caaggcgagg ttggtacggt gagcagatgt
    15301 accgctccat gcccgaaacg gtgaccgaac cgctggtccg cgccctccac cccgaaccgg
    15361 atgggatgct ggccaccacc gccatacctc cggtggacga gctgctcgga ggcctggcac
    15421 cggggacggt caccaccatc gacagctccg ataggatgct gttcgccctg gtcaacatgc
    15481 tcagtgtgaa cttcgtgcgc gaatggcatc gcgacgtgat ctgggtggat ggggggaact
    15541 ccgtgaatcc ctacgatctc acatacattt gcaagcgctt ccggctgcgg ccggaggagg
    15601 tgctcaacag cataagcgtg tcgcgggcgt tcaccgccta tcagatgtcc acccttgtgg
    15661 aggagtccct tgaggaggag cttgttcgca gcaggggggg aatggtcatc gtatcgtgct
    15721 acccggatct cttccaggac gcggacatgg actgggatga atcgttgaag ctcatggaaa
    15781 ggggcatggg caagctgcgt tcccttacgt ccaagcaccg caccgtgagc gtggtgacca
    15841 actacggctt agcgaagctg atgtaccgca aggggctgcg ctcccttttt tatgagaagg
    15901 cggatcgcgt ggtcagcgtg gagcatcgct ccggctacgt gcgcatctcg ttgcctcggg
    15961 aggagcggga gatcatgtac cgaccggtac cgccgaacca gaccaccttg gacgaatatc
    16021 ggaggaggtt ctgatggggc gcaccgtacc aacgtaccgc atggcctcgg aatccctctt
    16081 ccaaagctgg agcgattttc gacgcgccct tccgcaggag gatcgtgcgg cgttcgatcg
    16141 cctttccgac aaggtgcgca tgcacgcctc ggcttgtacg gtagcggcat tcctcgatcc
    16201 cctggaaggg gcgctgctct ctatcctctt ggagcaggag aaggaactgg agcgcctgcg
    16261 caagaaggcc tgatcgaatg cgcggatgga tcctggactg ctatgcggac gaggtcaaca
    16321 acgagatggt cacctgggtc aagaccccgg ccggcgtgga gcgcatcgcc gaccgcgagt
    16381 accgtccgtc cttttatgtg aagccctccg cggaacggaa acaggacctc ctcctcgggc
    16441 tgaaggaggc cggcgtggag catcgttggg agaggcaccg gacatggctc acgggacctc
    16501 tggaggacgt gctgaaggtc acgcccaaag ggtaccgcaa cctcatgcct ttggcgaaga
    16561 ccgtggacag gtgggggtgc caccgggacc acacgctttt caacgtggac atgcgcatgg
    16621 acacccgcta cttcctgtac cgcaaggtct tcccgatggg gctgatggat gtggagcgct
    16681 ggcataacct ggacgcaccg ttccgattgg actacccgat gcccgagctg cgcacctgtg
    16741 agcttacgtt ggagacctcc gctcgccggg gcatgccgac gttggaggac cgcctgaagc
    16801 gagccaccct cgacgacact gtcatggagg gcaaggaggc ggacatcctg gacatgcttc
    16861 aggcaaccct ccgcgccaag gaccctgacg tgctggtcac cgatggcggg gacagcttcc
    16921 accttccgtt cctggagagg tgcgccgcgc gcaacgagat ggagctggaa ttgggcaggg
    16981 aggggggaaa gcgcttgggc aagggcaaga gctacttcac ctatggccgg atcctctaca
    17041 aaccgcccgc gtacaagctg cgcgggcgca tccacctgga caagggctcc tcgttcatct
    17101 atcaggaggg gggcatggcc gggcttatcg acctctgccg gctggcgcgc atatcgatgc
    17161 aggacatggc ccgcatgtcc cctggcagcg ccatcagcgc catgcaggtg aacgaggcct
    17221 ccaagatggg ctcgctggtg atgtggaaga agaacctgcc tgaggggttc aagaccgccg
    17281 ccgagctggt ggcctgtgac cggggcggcc tcatctacga gccgaaggtc gggctgcatg
    17341 aacgcgtcca ggaggtggac ttcacctcgc tgtacccgag cataatggtc cgtcacaaca
    17401 tctccccgga gacgctgaac tgtgaatgct gttcggatgg cggggagccc gttcccggcc
    17461 ttggctattg gacctgcacc aagcgccaag ggttgctagg cagggtgctg gagcccctcg
    17521 tccgccggcg catcgccctc aagcggttga agcgggaggg ggggtgcagg agcgccattt
    17581 acgaccagcg ggccaaggtg ctcaagtggg tactggtgac ctgcttcggc tacacgggct
    17641 acaggaacgc gcgcttcggc cgtatcgagt gccacgaggc catcaccgcc tatggccggg
    17701 agatattgct cagatcggcg gaggtggccg agcaccgcgg cttccggatc ctgcacggca
    17761 tcgtggactc gctgtggctg gaggggaacg gagatgtcga ggcctattgc cgggagatag
    17821 ggcagcgcat gggcataccg ctggtctggg acggggagta caagtggatc gtcttcctgc
    17881 cgaacctgac caatggcgcc ggggcactga accgttacta tggcgccttc caggacgggg
    17941 agctgaaggt acgcggcata gccctccgca gaagggacac ttgcacgcta gccagggaac
    18001 tgcagcaggg catgcttgac agattggccc tgggagagga tgccgaagga ttccgtcatc
    18061 gcatcccgga ggcgctggat gttctggata cgttcgtacg gaacctcagg gacatggaga
    18121 cgccgctgga gaagctggtg ctttcgaagc gcataacgcg ggagctgagc gagtaccggc
    18181 agaggaacga ccagttccgc gcgctatgcc agctggagga ccggggcttc cgcacaccgc
    18241 ccggggaggc cgtcga
//
LOCUS       DAOSYN010000006        17611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2662_length_17611_cov_4.593928, whole genome shotgun sequence.
ACCESSION   DAOSYN010000006 DAOSYN010000000
VERSION     DAOSYN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 17611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17611
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2662_length_17611_cov_4.593928"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..898
                     /locus_tag="PLJ11_00570"
     CDS             62..898
                     /locus_tag="PLJ11_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOO03199.1"
                     /translation="MFEFPVLDNHVHLQPRGRNVEGVKDFLKAGGTHLIVSHMPYQEA
                     PVRSAEDFRSAYGITLRMVEVARSAGAGAFATLGPYPVQLLELAEAMPLERAVEVMKG
                     GMDIAASLVREGRAIALGEIGRPHFPVPDDVMAASNAILAYGMQLAKECGCPVVVHAE
                     SATPASMEELGAMADRVGLPRERVVKHYCGPLVAPHESRGLYPSVLATRPMVQEALAK
                     GDRFLLETDFMDDLERPGAVLAITTVPKRMRQLVEKGTDPSVLWKVNKDNPERVYGIT
                     IG"
     gene            complement(895..1167)
                     /locus_tag="PLJ11_00575"
     CDS             complement(895..1167)
                     /locus_tag="PLJ11_00575"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOO03200.1"
                     /translation="MVYRVSATFRGKVQGVYFRQYARRWAEMLSLSGYIRNMPDGSVL
                     AVFEGQRGAVQEIIRKLKEEHPAATVTSVDIKVEEPEGLEGFKIVH"
     gene            1462..2583
                     /gene="ftsZ"
                     /locus_tag="PLJ11_00580"
     CDS             1462..2583
                     /gene="ftsZ"
                     /locus_tag="PLJ11_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO03201.1"
                     /translation="MRSLVEEALAKDTAPVRAEPAPNFGYQQLDSTDAELIELLQRLK
                     TNIKIVGCGGGGSNTINRIFEENISGVETYAANTDAQHLLTTRAHHKILLGRRSTRGL
                     GAGALPQVGEQAAREAEEEIRKAVADAHIVFVTAGMGGGTGTGSASVVANIAKEMGAL
                     TIAVTTTPFKGEGRMRMENAEWGLQRLRSAADTVIVIPNDKLIELYPRLGLNQAFKMA
                     DEVLTKAIKGITELITKPGLVNLDFNDVRTIMKGAGVAMIGLGESDGHAEDRAKEAIE
                     RALSSPLLEVDITEANGVLVNVIGGPDMTISDAEFIAEEVQRRVSPTARIIWGAAVDP
                     TLEKTIRVMVVVAGVKSKQILGAPNETDKLKKVDLDFIR"
     gene            complement(2608..2802)
                     /locus_tag="PLJ11_00585"
     CDS             complement(2608..2802)
                     /locus_tag="PLJ11_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO03202.1"
                     /translation="MKNRLRIYRTMTNLTQEELAERLGVTRQTVIAIEKERYDPSLEL
                     AFKMAQLFKVAIEDIFQFEG"
     gene            3105..4208
                     /gene="ftsZ"
                     /locus_tag="PLJ11_00590"
     CDS             3105..4208
                     /gene="ftsZ"
                     /locus_tag="PLJ11_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO03203.1"
                     /translation="MKSFISDAMSRAGPASEASMHTFKAQTAEDEELEKILLSLKTNI
                     KILGCGGGGCNTIDRLYASSIKDAELYAVNTDAQHLLITRAHKKILLGRRSTKGLGAG
                     SLPQVGEEAAREAEEELRKVLMGADIVFITAGLGGGTGTGSAPYIAQLAKEMGALTIA
                     IVTSPFKAEGSIRAENAEWGMERLRAVADTVIVIPNDKLLELVPRYSINAAFKVADEV
                     LMRSIKGITELITRPGLVNLDFNDLKTIMKGGGVAMIGLGEGEDEDKVEQAIDEAINS
                     PLIDVDITGANGVLINVTGGEDMSVSEAEKVAEVIQSKVNPNARIIWGASVDPALENK
                     IRVMVVITGVKSKYILGPKETAAPKGMTVDFIR"
     gene            4262..4489
                     /locus_tag="PLJ11_00595"
     CDS             4262..4489
                     /locus_tag="PLJ11_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HOO03204.1"
                     /translation="MDIVDKSWEVQKRIEERVRLLGKGKYGRVLKMARKPTKDEYSKT
                     VMITGLGLFLIGGLGFAIYILVTRLPGWLGL"
     gene            4507..5367
                     /locus_tag="PLJ11_00600"
     CDS             4507..5367
                     /locus_tag="PLJ11_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HOO03205.1"
                     /translation="MNDQEINQSPQFGNAPLTLSGESGAQRVTAGSSAVWKLVLKPQQ
                     ANKMKVRVLLTIDYGSEDSPEWEVSISDTSGKLWESAKLTPPDIEFNMEGLGGKELTL
                     AAEAPRGARLDDSVHIKMQVFAEGQECGAMEFFANTMQSILILKTSIGHERAVVDGVA
                     GKAKAGDKGIFALLAPGKLDGYVFMEAMNTDLVRETCRGVRKAKGLVAGETKLSEIEH
                     FLTPKPLVSGISEGDVVELVAGPFKGEKARVQKIDESKEEITVELFEATVPIPVTVRG
                     DSVRVLEKER"
     gene            5372..5851
                     /locus_tag="PLJ11_00605"
     CDS             5372..5851
                     /locus_tag="PLJ11_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOO03206.1"
                     /translation="MAETIEVLVDGGKATPGPPLGPALGPTGINVVQVVSAINEKTKG
                     FAGMKVPVKVIIDQKTKTFEIKVGTPPTSALLFKELGIEKGAGAPKAAKVGNISIEQA
                     KKVAHMKQDSLTGADVKARVMEVIGTCASCGITVEGKEAKDIQAEIRAGKWDAQLQG"
     gene            5979..6617
                     /locus_tag="PLJ11_00610"
     CDS             5979..6617
                     /locus_tag="PLJ11_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020447957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOO03207.1"
                     /translation="MADKAIVTAVKKALERSPKRNFVQTVDLSINLKDVDMSIPKNRI
                     QEDIILPHGRGKPIKVCVIGSGEMLLKAKDVADRVVSVEELGTIADDKKQAKKMATEF
                     EYFIAEAPLMPTIGKRLGIVLGPRGKMPKPIPPGADPKPMIDNLRRSVPVRSRDRLTF
                     HTAVGTTDMPPEEIADNIEQIVKRLSTKLEKGTMNIRSAFVKTTMGPSEKVI"
     gene            6631..7626
                     /locus_tag="PLJ11_00615"
     CDS             6631..7626
                     /locus_tag="PLJ11_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOO03208.1"
                     /translation="MAHVAKWKRERVQGLVDQMVNSQVVGIVDLHGIPAAQMISMRAG
                     LRGKAEVSMTRNTLMDLAITEAAKKRPGLEQLRRMVEGQCAIVTTDENPFKLFRQMEG
                     TKTPAPAKPGDIAPEDIVVKAGETPFKPGPIIGELQKAGIPAAIESGKIIIKKDKVLV
                     PKGEPVPEEVAKVLAKLEILPMIVGMDLRAAFEDGVLYGKDVLNVPADYYTNLLGLAA
                     RNALGLSLSIAYVTPQTVTPLLSKAYREAMALSVASAFPTRESIGAILAKADAQMMTL
                     ASIIGLEDDRIKHRMAAVPAAKPVNEPKAAEAKKEEEEERVSEEEAAAGLSSLFD"
     gene            7639..7947
                     /gene="rpl12p"
                     /locus_tag="PLJ11_00620"
     CDS             7639..7947
                     /gene="rpl12p"
                     /locus_tag="PLJ11_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HOO03209.1"
                     /translation="MEYIYSALVLHAAGKQVSEDAVAAVLKSAGVEPDAARIKALTAS
                     LEGVNIDEAIASAMVAAAPVAAAAAPAAAPAAPAKEEPKEEEHVSEDEAAAGLSALFG
                     "
     gene            8099..9049
                     /locus_tag="PLJ11_00625"
     CDS             8099..9049
                     /locus_tag="PLJ11_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HOO03210.1"
                     /translation="MRLGEIYRFAVEVAKGSDPRTADELEDDLRRTEERYRKMEGEER
                     ARFDLDSLWNPYPDSRLVHGDPATEVGGVLWGIDISTGEMVLADRLRERGIPIDAVIG
                     HHPFGRARPAFGDALHLHEHIYERLGIPINVAEDIMAPRIREVHDMSAPANYDQAVDA
                     ARLLGLPLMCLHSVTDNLVDRHLSRLVEREGPRTVGDVVDLLQGQPEHQHAARNNNPP
                     EVYVGDRKRRAGKVVVRMTGGTSGPKELYEKLADAGVGTVVFMHIPESHLEEARKHHM
                     NVVIAGHVASDSMGMNLLADRLEERGVEVTPCSGFIRVRR"
     gene            9054..10208
                     /locus_tag="PLJ11_00630"
     CDS             9054..10208
                     /locus_tag="PLJ11_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO03211.1"
                     /translation="MRPPRLFKDQSKLSFDYVPDALCHREEQLERLWMLFRPVLEGSM
                     SQTAFLIGSVGTGKTATSKRFCLDLMKAAQEKGRAMDFIMVNCRQRPTEVAVLRRIVM
                     HFDENFPDRGFSSEEMLRSIRRHLEKRKVHLVVVLDEADVLLAKGAGDLVYQLTRFDE
                     EMVGGRPSMSVIMISQKNVLDMLDAASSSTFRRANAIRFDRYSVRELADILRARAELA
                     FYPGTVRPDSLDLMADIAGEFGDARFAIELLDKAGMLAEEEGAERVAPEHVRGAKALT
                     YSVVTESKLEDLDKQHRLVLLAVARAMKDQAYVRTPEAEAMYRLVAEEHGERPRGHTM
                     FWTYLQDLTNQGLVGTRVVNEEAGGRTTYISLPDVPAKVIRQRLEEGLGD"
     gene            10253..11362
                     /gene="gcvT"
                     /locus_tag="PLJ11_00635"
     CDS             10253..11362
                     /gene="gcvT"
                     /locus_tag="PLJ11_00635"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOO03212.1"
                     /translation="MKRTPLYQRHLAAGARMVEFAGWEMPIQYSGIIEEHRHVRKRCG
                     VFDVSHMGDLLVRGPQAASQLDYLLTNPIGKAPVGKAVYGHLLSTDGQIIDDVITYHI
                     ADGAYLMVPNAATVDRVREWVRGNTRDLEVLDLSTRLACLAVQGPLAEGVLQGMCSTD
                     LSTLRRFQGRFAFLTGRNSPFLPDLFPGRGEGVGCYIARTGYTGEDGFEVLVEDKDAG
                     WLWDRLLEGGAVRPIGLGARDTLRLEMGYLLSGTDFDGSQTSLQTGPPWVVKMDHPFI
                     GRDVLILQRERKYDVLVALRLEGRGVPRHGYAILEAGRPVGKVTSGTMSPCLRTGIAL
                     GYVPQRLAAVGTRLEVQVRDEAVPARVVELPFYRK"
     gene            11367..12419
                     /locus_tag="PLJ11_00640"
     CDS             11367..12419
                     /locus_tag="PLJ11_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO03213.1"
                     /translation="MNELVRSLIELLSVRSDTPEGRKALFELVRSRLEDLGLSVVTGG
                     AADSPAMLAANGVGGVAFAAHLDTVPAGEGWTRRDGEVVGDRVYGLGAADMKGGTAAM
                     LAAARALKEEDVPFSLLVTTDEEELMTGAEFLAKRPEVRLASGIVVGEPTDLRVAWKE
                     KGISRLTLTAHGVAAHASMPWQGDNAIMRMVGLLERLRPMLEVPDGPTEGLTLVVSNI
                     KGGTRSNIIPDRCDAQLNARFPFPVTGKEVRARLVRALEGEEYDLAVQYELPAFETPP
                     GSSLIKALLEVGAAGIFAVPYATEAAIYSAVNPLVAVCGPGSPGLAHSKDENVEARQL
                     EGAFHLYRALGRRLQG"
     gene            complement(12410..13708)
                     /gene="asnS"
                     /locus_tag="PLJ11_00645"
     CDS             complement(12410..13708)
                     /gene="asnS"
                     /locus_tag="PLJ11_00645"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOO03214.1"
                     /translation="MTAFTSIGSLLSTPVLEGQVALRGWVYRTRSSGKIVFAVLRDST
                     GIIQVTIKKGNLPDAQFEEAAAASIESSVEVTGTMHQDGRAPGGYEVLATSFKVVGPA
                     LPFPITEYQSEELLLDNRHLWIRSREQTAVMKVKATLLAGAREWLAEQGFTEVTPPIL
                     TQNACEGGVTLFKLRYFDRDVFLSQSAQMYLESLIFALEKVYSITPSFRAEKSRTPRH
                     LTEYWHLELEEAWTDNEGNMRIQEELVSAMVAKALKERGAELELLKRDIEPLKAVQPP
                     FERLRYADMIALLRDKGFAIEFGADLGAAEERAITEDKGAPVFVTNFPKQCKAFYMKE
                     DPDDPRTYKCADLLAPFGFGEVIGGSERETDLAKLLQRMEEQGIPQEPYRWYLDLRRF
                     GSVPHSGFGLGVERMVKWVCNLEHIRDAIPYPRTVARAYP"
     gene            13833..14222
                     /locus_tag="PLJ11_00650"
     CDS             13833..14222
                     /locus_tag="PLJ11_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03215.1"
                     /translation="MVKAKRSALAGMDGKRNELGGPSFSRSALETLMSDPDFAVLAAG
                     GSAAVVHHEEGGRSRMLLLIGRPTAVLLEEAEASARQAGAARVMVEVSPDSPVLPALE
                     AMGYHRAGEAADHFGRGRPAVLMEKQL"
     gene            14374..14541
                     /locus_tag="PLJ11_00655"
     CDS             14374..14541
                     /locus_tag="PLJ11_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03216.1"
                     /translation="MEESLCNVEFLKDNNNFVARVQSELGGLREYQSNSLEEVLEQVI
                     IDLQEEFENAM"
     gene            14622..14942
                     /locus_tag="PLJ11_00660"
     CDS             14622..14942
                     /locus_tag="PLJ11_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOO03217.1"
                     /translation="MQQSENDPILSSYYGDEEVTAIITLKVEAKDANAVCEAISMQKP
                     ALDVFLVTGDTDIVVKAKFRSYTHLKRFLLEDLSTVKGVKDTKTLMVVAIYKENGELR
                     TGGE"
     gene            14974..15249
                     /locus_tag="PLJ11_00665"
     CDS             14974..15249
                     /locus_tag="PLJ11_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HOO03218.1"
                     /translation="MEYDAKTKLNQVIDVLDQLSEDTSVPRNIRRGATEAKARLSQEG
                     DAMDVRVASAIGKLDDLANDPNIPMHGRTVIYRIISELENLLRTVSK"
     gene            15288..15359
                     /locus_tag="PLJ11_00670"
     tRNA            15288..15359
                     /locus_tag="PLJ11_00670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15321..15323,aa:Gly,seq:ccc)
     gene            complement(15372..17234)
                     /locus_tag="PLJ11_00675"
     CDS             complement(15372..17234)
                     /locus_tag="PLJ11_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO03219.1"
                     /translation="MIATDGLMFEVGIIMLVAFIGAAVASKAKQSVILGYILAGMLIG
                     PYISVELFGYTYDGLVKDTEFIQLLSSLGLTLLMFFVGLGFSITKLKRTKAPAIILAM
                     MDVGLGMFLGILIGYALGWPIVDTVFLAGVISMSSVAITGKALEEQGRFSSPETEYLL
                     GTVIVESFLSMVLLTIVGGLMFKSNADDMNITRLVVGILAFYGFFLFLAIVVVPRTVK
                     YFESIKSDELFVLLALGLVFLSAALAEAAGVPAIIGAFFLGMVFAETRLAERIEDRIT
                     PFRDALVAVFFIAFGMMIDLSMLRTVVPILLIAVPVTLFYESVVLSSISYLLGFPSKA
                     ATFIGTAMTGRSSEAIMYASVGSSSPAVTKGSELNPFAGAFCFIMSMVAPSFMKHSAG
                     FTRTLIRLTPKFLVYGGSLINRTMGKVILPSTIRLFERTRRLEALIAVYFIDLVFIMA
                     LQFPYDMLAFVLGGLIVAVIYATLESDMYSIVRTVNYDNLGVVTKDLGHISRYISGFV
                     SLSLVTILSLAALFPIWWASSLLILSIYFAIVLWLSWSAYRITRTPALNIDARPVRRR
                     PQKGQRPFQPEFRPPSPGPDPGWDLGPGEPPSARGPAPAARTEEKGDDDRWGRL"
BASE COUNT         3542 a         5186 c         5906 g         2977 t
ORIGIN      
        1 acgtgggcgc agcggtcgac aagatcaccg aggagctgaa ggcggcgggc ctgctgaggt
       61 gatgttcgag ttcccggtcc tcgacaacca tgtccacctt cagccgagag ggcggaacgt
      121 cgagggcgta aaggatttcc tcaaggcggg cggcacccat ctgatcgtga gccatatgcc
      181 gtaccaggag gccccggtac ggagcgcgga ggactttcgt agcgcctatg ggatcacact
      241 gaggatggtc gaggtcgccc gctccgccgg cgcgggcgcc ttcgctaccc tgggaccgta
      301 ccccgttcag ctgcttgagc tcgccgaggc gatgcccctg gagagggcgg tggaggtgat
      361 gaaggggggg atggacatcg ccgcgtccct ggtccgggag gggagggcga tagcgctggg
      421 ggagataggc cgcccgcact ttcccgttcc cgatgatgtg atggccgcct cgaacgccat
      481 attggcgtac ggcatgcagc tggccaagga gtgcgggtgc ccggtggtcg tccacgccga
      541 gagcgccacc ccggcgagca tggaggagtt gggggcgatg gccgacaggg tggggctgcc
      601 ccgggagagg gtggtcaagc actactgcgg gccgttggtc gccccgcatg agagccgggg
      661 gctttacccc tccgtgctgg ccaccaggcc aatggtgcag gaggccttgg ccaaagggga
      721 ccgcttcctc ctggagaccg atttcatgga cgacctggag aggccggggg cggtgctggc
      781 gatcaccacc gtgccgaagc ggatgcggca attggtggag aaggggaccg acccatcggt
      841 actatggaag gtcaacaagg acaacccgga gagggtctac gggatcacga tcggctaatg
      901 cactatcttg aacccttcga ggccctccgg ctcctcgacc ttgatgtcca cggaggtcac
      961 ggtggcggcg ggatgttcct ccttaagctt tcggatgatc tcctggaccg cccctcgttg
     1021 tccttcgaag acggctagga ccgaaccgtc aggcatgttc cttatatacc cgctcagaga
     1081 gagcatttcc gcccaccgtc gggcgtattg gcggaagtag acgccctgca ccttccccct
     1141 gaacgtggcc gatacgcggt agaccatgct ccttacattc gtgcgtggac gtaataattt
     1201 atcttgcagg gggcgagggt gacgccccct cgtcaagctg cgcgccctcc gtccggtcgc
     1261 gcatgtcttg cgatggcctt tgcgatcgcc cgcggcgcct tcttcgaggg cccgggcaca
     1321 tcgacgccgt aatgccgctc ggcccaacga acggccattg caccagggag gaacaacaat
     1381 ggaggaacaa caatatttaa tagagatggc atatgggggg acaaatcgtg ggaagttcgt
     1441 ggcaagaggg tgggatggtt catgagatcg cttgttgagg aagctttggc gaaggataca
     1501 gctccagtaa gggcggagcc tgccccgaat ttcggatatc agcagttgga ctccacggac
     1561 gccgaactca tcgagctcct gcagagactt aagaccaaca tcaagatcgt gggctgtggg
     1621 ggcgggggct ccaacaccat caaccgcatc ttcgaggaga acatctccgg cgtggagacc
     1681 tatgccgcca acacagatgc gcagcatctg ctcaccaccc gcgcccatca caagatcctg
     1741 ctggggcgga ggagcacccg gggcttgggc gccggggcgc ttccgcaggt gggcgaacag
     1801 gctgcccggg aggccgagga ggagatccgg aaggcggtcg ctgatgccca catagttttc
     1861 gtcaccgccg gcatgggcgg cggcaccggg accggttccg cttccgtggt cgccaacatc
     1921 gccaaggaga tgggggcctt gaccatcgcc gtcaccacca ccccgttcaa gggcgagggc
     1981 aggatgcgca tggagaacgc ggagtgggga ctgcagcggc tgcgcagcgc cgccgatacc
     2041 gtcatagtca tcccgaacga caagctcatc gagctgtatc cccggctggg actgaaccag
     2101 gccttcaaga tggccgacga ggtcctcacc aaggccataa agggcataac cgaactcatc
     2161 accaagcctg gtctggtcaa cctggacttc aatgacgtcc gcaccatcat gaaaggtgct
     2221 ggcgtagcaa tgatcggcct gggagagagc gacggccacg ctgaggaccg cgcaaaggaa
     2281 gcgatcgagc gcgccctcag ctccccgctg ctggaggttg acatcaccga ggccaacggc
     2341 gtcctggtga acgtcatcgg cgggccggac atgaccatct cggacgcgga gttcatagcc
     2401 gaggaggtgc agcggagggt cagccccacg gccaggataa tctggggcgc cgcggtcgac
     2461 ccgacccttg agaagaccat ccgtgtcatg gtcgtcgttg ccggggtcaa atccaagcag
     2521 atcctgggcg ccccgaacga gacggacaaa ctgaagaagg tcgacctgga cttcatccgc
     2581 tgataccatg ccgggggcaa cccccgttca tccttcgaat tggaatatat cctcgatggc
     2641 caccttgaag agctgcgcca tcttgaacgc cagctccagc gaggggtcgt acctctcctt
     2701 ctcgatggcg atgacggtct gccgggtgac gcccagcctc tccgccagct cctcctgtgt
     2761 gagattggtc atggtgcggt agatcctcaa tcggttcttc atatcgccct cttatgtcaa
     2821 gctatattta cattttgaat attaatcttt actttttgta aaatacaaaa tacattttgt
     2881 aatttaatat atacatcaaa caaataaaat attctataaa gaatcttatt ttatagaaaa
     2941 taattgaatg gattgaacga taaatcctta catatattca ttgaattgaa ttgtaattcg
     3001 ctgatggcac cgaaatgtat taaaatctac attgcctcat gtattttaga cgcggagcag
     3061 ccgagggctt cagccgccag ccgcttccgt tcgaggcccc ctccatgaag tcattcatca
     3121 gcgacgccat gtcccgagcc ggtccagcaa gcgaggctag catgcacaca ttcaaggccc
     3181 agacggccga ggatgaggag ctggaaaaga tcctgttgag cctgaagacc aacatcaaga
     3241 tccttggatg cggtggtgga ggctgcaata cgatagaccg tctgtatgcc agcagcatca
     3301 aggacgctga gctgtacgcc gtcaataccg acgcgcagca tctgctgatc actagggccc
     3361 acaagaagat cctgctcggc cgccgaagca ccaagggctt gggcgccgga tcgctccccc
     3421 aggtgggaga ggaggccgcc cgggaggccg aggaggagct gcgcaaggtg ctgatgggag
     3481 cggacatagt gttcatcacc gcgggcctgg gagggggcac cggcactggc tctgccccct
     3541 acatcgccca gctggcaaag gagatggggg ccttgacgat cgccatcgtc acctccccct
     3601 tcaaggccga gggatcgatc cgcgcggaga acgccgaatg gggcatggag cgcctgcggg
     3661 ccgtggcgga tacggtcatc gtcatcccca acgacaagct attggagctc gtgccccgct
     3721 attccatcaa cgccgccttc aaggtggccg acgaggtgct catgcggtcc atcaagggga
     3781 taacggagct gatcacccgg cccgggctgg tcaacctgga cttcaacgac ctgaagacca
     3841 tcatgaaggg cggaggcgtt gccatgatcg gcctggggga gggcgaggac gaggacaagg
     3901 tcgaacaggc catcgacgag gcgatcaact ccccgctgat cgacgtggac atcactggcg
     3961 ccaatggggt gctcatcaat gtcacgggcg gtgaggacat gagcgtcagc gaggccgaga
     4021 aggtcgccga ggtcattcag agcaaggtca atcccaacgc gaggatcatc tggggcgctt
     4081 ccgtcgaccc ggccctggag aacaagatca gggtgatggt cgtgatcacc ggggtcaagt
     4141 ccaagtacat cctcggaccg aaggagaccg ccgcgcccaa ggggatgacg gtggacttca
     4201 tccggtaaag aaaggattat ttataaggaa ggattagggc gtaatccaag agtgcgtaca
     4261 aatggacatt gtcgacaaat cctgggaggt ccaaaagagg atcgaggaac gggtcagact
     4321 gcttggcaag ggcaagtacg gccgcgtcct gaagatggcc agaaagccaa cgaaggacga
     4381 gtactccaag acggtcatga tcaccggcct aggcctcttc ctcattggcg gccttggctt
     4441 cgccatatac atattggtga cccggcttcc gggatggctc ggcctttgat cccgggaggg
     4501 ctgcagatga acgatcagga aatcaatcaa tccccgcagt tcgggaacgc cccgctcacc
     4561 ctttcagggg agagcggcgc ccagagggtg accgccggaa gcagcgccgt ctggaagctg
     4621 gtcctcaagc cccagcaggc gaacaagatg aaggtgcggg tccttctgac catcgactac
     4681 ggcagcgagg actcgcctga gtgggaggtg agcatctccg acacctctgg aaagctgtgg
     4741 gagagcgcca agctgacccc tccggacatc gagttcaaca tggaggggct cggcgggaag
     4801 gagctgacac tggcagcgga ggccccgcgc ggggcgcgtc tggacgacag cgttcacatc
     4861 aagatgcagg tcttcgccga ggggcaggaa tgcggcgcca tggagttctt cgccaacacc
     4921 atgcagtcca ttctgatcct gaagacctcc atagggcatg agagggcggt cgtggacggg
     4981 gtggccggca aggccaaggc cggggataag ggcattttcg ccctgctcgc cccgggtaag
     5041 cttgacggct acgtgttcat ggaggccatg aacacggacc tggtcaggga gacctgccgg
     5101 ggggtgcgta aggccaaggg gctcgtggcc ggggagacca agctctcaga gatagagcac
     5161 ttcctgaccc ccaagcccct ggtctctggg atatccgaag gggacgtcgt ggagctggtc
     5221 gccggcccct tcaagggcga gaaggcccgc gtgcagaaga tcgacgagag caaggaagag
     5281 atcacggtgg agcttttcga ggccaccgtc cccatccccg tcaccgtccg cggggacagc
     5341 gtgagggtcc tcgagaagga gagatgatac aatggcagag actatcgaag tgctagtgga
     5401 tggcggaaag gccactcccg gtccgccgct ggggcccgcc ctgggcccga cgggcataaa
     5461 cgtcgttcag gtcgtgagcg cgatcaatga gaagaccaag gggttcgccg gcatgaaggt
     5521 cccggtgaag gtcataatcg accagaagac caagaccttc gagatcaagg tcggcacgcc
     5581 gcccacctcg gccctgctgt tcaaggagct gggcatcgag aagggcgctg gggcccccaa
     5641 ggccgccaag gtcggtaaca tctccatcga gcaggccaag aaggtcgccc acatgaagca
     5701 ggactccctg accggcgccg acgtcaaggc gagggtcatg gaggtcatcg gcacctgcgc
     5761 ctcctgcggc attaccgtgg agggcaagga ggccaaggac atccaggcgg agatcagggc
     5821 gggcaaatgg gatgcccagc tgcagggctg agatccctcc gccccaaacg cctttcccct
     5881 gttttacgat atctttataa atggcagggt gattaggggg cttcaccaaa ctgtaggaga
     5941 gccttcgtag gctcgatgga actacaggag ggatcgaatt ggcagataaa gcaatcgtta
     6001 cggccgttaa gaaagccttg gagcgttctc caaagcgcaa ctttgtgcaa acggtcgacc
     6061 tgtccatcaa cctgaaggac gtcgatatgt ccatacccaa gaacaggatc caggaggaca
     6121 tcattctgcc gcacgggagg ggaaagccca tcaaggtctg tgtcatcggc agtggagaga
     6181 tgctgctcaa ggccaaggac gtggccgacc gggtcgtgtc cgtggaggaa ctgggcacca
     6241 tcgccgatga caagaagcag gccaagaaga tggccaccga gttcgagtac ttcattgcgg
     6301 aggccccatt gatgcccacc atcggtaaga ggctgggcat agtgcttggt ccccgcggga
     6361 agatgccgaa gccgatcccc ccgggcgcgg accccaagcc catgatcgac aacctgcgca
     6421 ggtcggttcc ggtcaggagc cgggacagat tgacgttcca caccgctgtc gggaccacgg
     6481 acatgccgcc agaggagata gcagacaaca tcgagcagat cgtcaagagg ctgtcgacca
     6541 agctcgagaa gggcaccatg aacatccggt ccgccttcgt gaagaccacc atgggaccct
     6601 ccgagaaggt gatttgagga ggctccgacc atggcacacg tagcgaaatg gaagagggag
     6661 agggtgcagg ggctggtcga ccagatggtc aacagccagg ttgtcgggat agtcgatctg
     6721 cacggcattc ccgccgccca gatgatctcc atgcgcgccg gtctgcgcgg gaaggccgag
     6781 gtgagcatga ccaggaacac cctcatggac ctggccatca ccgaggccgc caagaagcgg
     6841 ccgggcctgg agcagctgag acggatggtg gagggacagt gcgcgatcgt cactacggac
     6901 gagaacccgt tcaagctgtt ccgccagatg gagggcacca agaccccggc cccggccaag
     6961 cccggggata tagcgccgga ggacatagtg gtgaaggcgg gggagacccc attcaagccc
     7021 ggcccgatca tcggggagct gcagaaggca ggcatcccgg cggccataga gtccgggaag
     7081 atcataatca agaaggacaa ggtcctcgtc cccaagggcg agcccgtccc cgaggaagtg
     7141 gcgaaggtac tggccaagct ggagatcctg cccatgatcg tgggcatgga cctcagggcc
     7201 gccttcgagg acggggtgct ctacgggaag gacgtcctga acgtcccggc ggactattac
     7261 accaacttgc ttggtctggc cgccaggaac gctctgggcc tgagcttgtc aatagcctat
     7321 gtcacgcctc agaccgtcac gcccttgctg tccaaggcct accgcgaagc gatggccctg
     7381 agcgtggcga gcgcgttccc caccagggag agcatcggtg cgatccttgc gaaggccgac
     7441 gcgcagatga tgaccttggc ctcgatcatc ggtttggaag atgaccggat aaagcacagg
     7501 atggctgcgg tccccgcggc caagccggtg aacgagccca aggccgctga ggccaagaaa
     7561 gaggaagagg aggagcgggt cagcgaggag gaagccgccg ctggcctgag ctctctgttc
     7621 gattgaggag gtaaggaaat ggaatacata tacagtgctt tggtccttca cgccgctggg
     7681 aagcaggtgt ccgaggacgc ggtcgccgct gtcctgaaga gcgccggtgt cgagccggac
     7741 gcagccagga taaaggcctt gaccgcctcc ctggagggtg tgaacatcga tgaggctatc
     7801 gcctccgcga tggtcgccgc cgcgccagtc gctgccgctg ctgcgcctgc cgccgctccg
     7861 gccgccccgg ccaaggaaga gcccaaggaa gaggagcacg tcagcgagga cgaggccgca
     7921 gccggtctgt ccgcgctgtt cggctgaaca ggccagacaa aggccggtcc ccagttgccg
     7981 agccttctca gggccttaaa cccttttttt ccacattttc tgttctttcc cattttactc
     8041 gcggtgcagg cgtccgccgg acgaacagtt tatcagctta ccggacctct cagttccgat
     8101 gaggctagga gagatctacc ggttcgcagt tgaggtggcg aagggcagcg acccacggac
     8161 cgcggatgag ctggaggacg acctccggcg gacggaggag cgctatcgga agatggaggg
     8221 ggaggagagg gcgcgcttcg acctggattc gctgtggaac ccctatcccg actccaggct
     8281 ggtgcatggc gatccggcga cggaggtcgg aggggtgctc tggggaatag acatctccac
     8341 cggggagatg gtcctggccg accggttgag ggagaggggg atacctatcg acgcggtcat
     8401 cggccaccac cccttcggca gggcgaggcc agccttcggg gacgccctcc atctccatga
     8461 gcacatctat gagcggctgg ggatcccgat caacgtcgcc gaggacatca tggccccccg
     8521 catccgcgag gtgcatgaca tgagcgcccc ggccaactac gaccaggccg tggatgccgc
     8581 ccgtctcctg ggcctgccgt tgatgtgcct tcactccgtc accgacaacc tggtcgacag
     8641 gcacctctcc cgattggtgg agagggaggg gcccaggacg gtgggggacg tagtggacct
     8701 gcttcagggg cagccggagc accagcatgc cgcccgcaac aacaatcccc cggaggtgta
     8761 cgtgggggac aggaagcggc gggcgggcaa ggtggtcgtg cggatgacgg ggggcacctc
     8821 gggccccaag gagctctacg agaagctggc cgacgccggc gtggggaccg tggtgttcat
     8881 gcacatcccg gagtcccatc tggaggaggc gaggaagcac cacatgaacg tggtcatcgc
     8941 cgggcatgtg gcctccgact ccatgggcat gaacctgctc gccgaccggc tggaggagag
     9001 gggggtggag gtcaccccct gctccgggtt catcagggtc agaaggtgag cgggtgcgcc
     9061 ctccacggct gttcaaggac caatcgaagc tctcgttcga ctacgttcca gacgcgctct
     9121 gccaccgcga ggagcagctg gaacgcctgt ggatgctctt ccggcctgtg ctggaaggga
     9181 gcatgtccca gacggcgttc ctcatcggca gcgtgggcac tgggaagacg gcaacgtcca
     9241 agcgcttctg cctggaccta atgaaggccg cccaggagaa gggccgggca atggacttca
     9301 tcatggtcaa ctgccggcag cgccccaccg aggtggcggt gctccggcgc atcgtcatgc
     9361 acttcgacga gaacttcccc gacagggggt tctcctccga ggagatgctg cgctccatcc
     9421 gccggcacct cgagaagcgg aaggtgcacc tggtggtggt attggacgag gcggatgtgc
     9481 tgctggcgaa gggcgccggc gacctcgtct accagctcac ccgcttcgac gaggagatgg
     9541 tgggcgggcg cccgtcgatg tccgtcataa tgatctccca gaagaacgtg ctggacatgc
     9601 tggatgccgc gtcgtcctcg accttccgca gggccaacgc catccggttc gaccggtact
     9661 ccgtgcggga gctggccgac atcctgaggg cgcgcgcgga gctggccttc tacccgggga
     9721 cggtgcgccc ggactccctg gacctcatgg cagacatcgc cggggagttc ggggacgccc
     9781 gctttgccat cgaactgctg gacaaggccg gaatgctcgc ggaggaggag ggcgccgaac
     9841 gcgtcgcccc cgagcatgtc aggggggcga aggcgctcac ctactcagtg gtcacggaga
     9901 gcaagctcga ggacctggac aagcagcaca ggcttgtcct gttggccgtc gcccgggcga
     9961 tgaaggacca ggcgtacgtg cggacgccgg aggcggaggc gatgtaccgg cttgtggccg
    10021 aggagcacgg ggagaggccg cgcgggcaca cgatgttctg gacctacctc caggacctga
    10081 ccaatcaggg gcttgtcggg acaagggtcg tcaacgagga ggcgggcggc cgcaccacgt
    10141 acatctccct cccggacgtg ccggccaagg tcatccggca acggctcgag gaggggttgg
    10201 gggactgacg gaaagctatt gataatccgc ccgccattca aaagaggagc gtatgaagag
    10261 gacgccattg taccagaggc accttgcggc aggggcgagg atggtggagt tcgcggggtg
    10321 ggagatgccc atccagtatt ccggcatcat cgaggaacat aggcacgtgc ggaagcggtg
    10381 cggggtgttc gacgtgtcgc atatgggcga tctgctggtc agggggccgc aggccgcgag
    10441 ccagctcgat tatcttctga ccaatccgat cggcaaggcg ccggtgggca aggcggtgta
    10501 cggacatctg ctgagcacgg atgggcagat catcgatgac gtcattacct accacatcgc
    10561 cgacggggcg tacctcatgg tccccaacgc cgccaccgtg gaccgcgtgc gggaatgggt
    10621 gagggggaac accagggacc tggaggtgct ggacctgtcc acccggctgg cgtgcctggc
    10681 cgtgcagggg cccctggccg agggagtgct ccagggcatg tgctccaccg acctctccac
    10741 cctgaggcgg ttccagggcc gcttcgcctt cctcacgggg aggaactccc cgttcctgcc
    10801 cgacctcttt ccaggcaggg gggagggagt ggggtgctac atcgcccgga cgggctacac
    10861 cggcgaggac gggttcgagg tgctcgtgga ggacaaggac gcgggctggc tttgggaccg
    10921 gctgctggag ggcggggcag tcaggccgat agggctggga gcgagggaca ccctgcggct
    10981 ggagatgggc tacctgctgt cgggcacgga cttcgacggg agccagacgt ccctgcagac
    11041 agggccgccc tgggtggtca agatggacca ccctttcata gggcgggacg tccttatcct
    11101 tcagcgcgaa cgcaagtacg acgtgctggt cgcgctgcgg ctcgagggga ggggcgtgcc
    11161 tcgccatggg tatgccatcc tggaagcggg caggccggtc ggcaaggtca ccagcggcac
    11221 gatgtcgccc tgcctgagga cgggcatagc cctgggctac gtaccccagc ggctggccgc
    11281 cgtcgggacc cgattggagg tccaggtccg cgacgaggcc gttcccgcca gggtcgtgga
    11341 gcttccattc tatcgcaaat gataccatga acgaactggt ccgcagcctg atcgagctgc
    11401 tcagcgtgcg cagcgatacc cccgagggca ggaaggccct gttcgagctg gtgcgctcga
    11461 ggctggagga cctcggcctc tcggtcgtga ccgggggggc ggcggacagc ccggccatgc
    11521 ttgcggccaa tggggtcggg ggggtcgcct tcgccgccca tctggacacc gtcccggccg
    11581 gggagggctg gacccggcgg gacggggagg tcgtggggga ccgggtctac gggctgggcg
    11641 cggcggacat gaagggcggg acggcggcga tgctggccgc ggcgcgggcc ctgaaggagg
    11701 aggatgtgcc gttctccctg ctcgtcacca ccgacgagga ggagctgatg accggggctg
    11761 agttcctggc caagcgtccg gaggtccggc tcgccagcgg gatcgtagtc ggggagccga
    11821 ccgacctccg ggtggcctgg aaggagaagg gcatctcccg tctcacgctc acggcgcacg
    11881 gcgtggccgc ccacgcaagc atgccctggc agggggacaa cgccatcatg cggatggtcg
    11941 ggctcctgga gcggctgagg ccgatgctgg aggtacccga cggacctacg gaggggctta
    12001 cgctcgtcgt ctccaacata aaggggggga cgcgcagcaa catcatcccg gaccggtgcg
    12061 acgcccagct gaacgccagg ttcccgttcc cggtgacggg gaaggaggta cgggcgcgtc
    12121 tcgtgcgcgc gttggagggg gaggagtacg acctagccgt acagtacgag cttccggcct
    12181 tcgagacccc gcctggatcg tcgttgataa aggcgctgct ggaggtcggc gccgcaggca
    12241 tattcgcggt gccctatgcg acggaggccg ccatctactc ggccgtcaac cccctcgtgg
    12301 ccgtctgcgg cccgggcagc ccgggcctcg cccatagcaa ggacgagaac gtggaggcca
    12361 ggcagctgga gggagcgttc catctatacc gtgccctagg aagacggctt cagggatagg
    12421 ccctggccac ggtcctcggg taggggatgg cgtcccgaat gtgctccagg ttgcacaccc
    12481 atttcaccat gcgctccacg cccaggccga agccggagtg cgggaccgag ccgaagcgcc
    12541 ggaggtccag gtaccagcgg tacggctcct gggggatgcc ctgctcctcc atccgctgca
    12601 ggagcttggc gaggtccgtc tccctctcgc tgcccccgat gacctccccg aacccgaagg
    12661 gggccaaaag gtcggcgcat ttgtaggtgc gcgggtcgtc cgggtcctct ttcatgtaga
    12721 aggccttgca ttgcttcggg aagttggtca cgaagacggg ggcccccttg tcctccgtga
    12781 tcgccctctc ctccgcggcc cccaggtcgg ccccgaactc gatggcgaag cccttgtccc
    12841 tgagcagggc gatcatgtcg gcatagcgca ggcgctcgaa gggcggctgg accgccttaa
    12901 ggggctcgat gtccctcttg agcagctcca gctccgcccc cctttccttc agcgccttgg
    12961 ccaccatggc ggacaccagc tcctcctgga tccgcatgtt gccctcgttg tccgtccacg
    13021 cctcctcgag ctcgagatgc cagtactcag tgagatgcct gggcgtccgg gacttctcgg
    13081 cccggaagga gggtgtgatg gaatacacct tctccaaggc gaagatcagc gattcgaggt
    13141 acatctgggc gctttggctg aggaagacgt cccgatcgaa gtaccgcagc ttgaagagcg
    13201 tcaccccgcc ttcgcacgcg ttctgggtga ggatcggggg ggtcacctcc gtgaacccct
    13261 gctccgccag ccattccctc gcccccgcca agagcgtggc cttcaccttc atgacggcgg
    13321 tctgctccct ggaccttatc cagagatggc ggttgtcgag gaggagctcc tcgctctggt
    13381 actccgtgat ggggaacggc agcgcggggc cgaccacctt gaaggaagtg gccaacacct
    13441 cgtacccgcc aggcgcccgg ccgtcctgat gcattgttcc cgtgacctcc accgatgact
    13501 ctatggaggc ggccgcggcc tcctcgaact gggcgtcggg caggttgccc ttcttgatgg
    13561 tgacctggat gatgcccgtg gagtcccgca gcacggcgaa gacgatcttg ccgctgctgc
    13621 gcgtgcggta gacccagccc ctcaatgcca cctggccctc caggaccggg gtgctgagca
    13681 gggaaccgat gctggtgaat gcggtcattg tgcacctggc tggtgaagaa ctgcatctat
    13741 gtattccatc gtttcggtcc tggggaactg atataagcca gggggccgat gccggggcgt
    13801 gcccgccgct gtcgagcgca ggaagggatg acatggtcaa ggcaaagagg agcgccctgg
    13861 cgggaatgga cggaaagcgg aacgaactgg gagggccgag cttctcccgc tccgccctgg
    13921 agacgctgat gtcggacccg gacttcgcgg tccttgcggc cggggggtcg gcggcggtcg
    13981 tgcaccatga ggaaggggga aggagccgga tgctgctgct gatcggccgg cccacggcgg
    14041 tcctcctcga ggaggcggag gcttcggccc ggcaggccgg ggcggcgagg gtcatggtgg
    14101 aggtgagccc ggacagcccg gtcctgccgg cgctagaggc gatgggatac cacagggcgg
    14161 gggaggccgc cgaccacttc gggagggggc ggccggcggt gctcatggag aagcaattat
    14221 gatattgtta tttttataga aaaagaaata acaatatttc cagtcgggta tctggctggc
    14281 ctgcggccgc cctcaagcca actattatat atggaaccgt tcattcgccc aaacagacca
    14341 acgcttatgg atgttggaca ggggtgaacg atcatggaag agtccctttg caatgttgag
    14401 ttcctcaaag acaacaacaa tttcgtcgca cgtgtgcaaa gcgagctcgg aggcctccgg
    14461 gaataccaaa gcaacagcct ggaggaggtc ctggaacagg tcatcatcga cctgcaagag
    14521 gagttcgaga acgcgatgtg atcgcttcca ttttttcacc gatctgatgt ccaacagggt
    14581 cgcgaaggcg gcccgatttg agcggccggg gagggttgca ggtgcagcag agcgagaacg
    14641 atccgatatt gtcctcatac tacggggacg aggaggtcac cgcgatcata accctcaagg
    14701 tggaggccaa ggacgccaat gcggtgtgcg aggccatatc catgcaaaag ccggcgctgg
    14761 acgtgttcct ggtcacgggg gacacggaca tagtggtcaa ggccaagttc cgtagctaca
    14821 ctcatctcaa gcggttcctg ttggaggatc tgtccaccgt gaagggggtc aaggacacca
    14881 agaccctcat ggtggtggcc atatacaagg agaacgggga gctgcggacc ggcggtgagt
    14941 agggaccgcc ccgatctagc aggaagtgat cgtatggagt acgatgcgaa aactaagctc
    15001 aatcaggtca tcgatgtctt ggaccagctc tcggaggaca cctccgttcc ccgcaacatt
    15061 cgccgcggag cgaccgaggc caaggccagg ctgagccagg aaggggatgc catggacgtg
    15121 cgggtggcga gcgccatcgg caagctggac gatctggcga acgaccccaa catccctatg
    15181 cacggccgca ccgtcatcta caggatcatc tccgagctgg agaacctgct gaggacggtc
    15241 agcaagtgaa aagcttttaa cgacattctg ttatctacta gcctcaggcg ggcatagtct
    15301 agtggttagg actgaagctt cccaagcttc gaacccgggt tcgaatcccg gtgcccgcac
    15361 ctattctggg ctcacagccg cccccatctg tcatcatccc ccttctcctc cgtccgggcg
    15421 gcgggcgccg gcccccttgc cgatgggggc tcccccggcc ccaggtccca tcccgggtca
    15481 gggcccgggg aggggggccg gaattcaggc tggaagggcc tctgcccttt ctgagggcgg
    15541 cgcctgactg gcctggcatc gatgttcagg gcgggcgtcc gggtgatgcg ataggcgctc
    15601 catgacagcc agagcacgat ggcgaaatag atcgagagga tgaggaggga ggaggcccac
    15661 catatcggga agagggcggc caacgagagg atcgtcacca ggctgaggct gacgaagccg
    15721 gagatgtacc ggcttatatg ccctaggtcc ttcgtcacta cgccaaggtt gtcgtagttc
    15781 acggtgcgca cgatggagta catgtcgctc tccagggtcg catagatgac cgccacgatg
    15841 aggccgccca ggacgaaggc cagcatatcg tagggaaact gcagcgccat tatgaagacc
    15901 aggtcgatga agtagaccgc gatcagcgcc tccagccggc gggtccgctc gaacagcctg
    15961 atggtggatg gtaggatgac cttgcccatt gtccggttga tcaaggagcc tccgtacacc
    16021 aggaatttcg gggtgagcct gatgagggtg cgggtgaacc ctgcactgtg cttcatgaac
    16081 gagggggcga ccatgctcat gatgaagcag aaggccccgg cgaaggggtt cagctccgag
    16141 cccttggtca ccgccggcga gctgcttccc acgctcgcgt acattatggc ctcggaggag
    16201 cggccggtca tggccgtgcc gatgaaggtg gccgccttcg aggggaaccc gagcaggtag
    16261 gagatggagc tgagcaccac cgactcgtag aatagggtga caggcacggc gatgagcagg
    16321 atggggacga cggtgcgcag catgctgaga tcgatcatca tgccgaacgc tatgaagaag
    16381 accgccacca gggcgtcccg gaagggggtg atgcggtcct cgatacgctc cgccagcctg
    16441 gtctcggcga agaccatgcc caggaagaac gcgcctatta tcgccgggac ccccgccgcc
    16501 tccgccagcg cggcggacag gaacacgagg ccgagggcca gcaggacgaa cagctcgtcg
    16561 ctcttgatgc tctcgaagta cttgaccgtc cggggcacga caacgatggc caggaagagg
    16621 aagaagccgt agaaggccag tatgcctact acgaggcggg ttatgttcat gtcgtcggcg
    16681 ttggacttga acatgaggcc gccgacgatg gtgagcagga ccatcgacag gaaactttca
    16741 actatgaccg tgcccagcag gtattcggtc tccgggctgg agaaccgccc ctgctcctcc
    16801 agggccttgc ctgttatggc cacggaactc atggatataa ccccggccag gaagaccgtg
    16861 tcgacgatcg gccagccgag ggcgtagccg atcaggatgc cgaggaacat cccgaggccg
    16921 acgtccatca tggccaggat tatcgctggg gccttggtcc tcttcaattt cgtgatggag
    16981 aagcccagcc ccacgaagaa catgagcagg gtgaggccca ggctggacag cagctggatg
    17041 aactcggtgt ccttcaccag cccatcgtac gtgtagccga agagctccac ggagatgtag
    17101 gggccgatga gcattcccgc aaggatgtac ccgaggatga cgctctgctt cgccttcgag
    17161 gccacggctg cgccgatgaa ggcgacgagc attatgatcc cgacctcgaa catcagaccg
    17221 tcagttgcta tcatctcgcc tacctacggg gatgcaccgg tcctgtccgg aggtgcatcc
    17281 cgttcctcgt tcggatatcg agggcctcgc cgacagagcg gtgcccctgg tgcatcccag
    17341 gccgctatag tattacatct tatttataat tgtccacatc tggccttttc catctgcccc
    17401 atttccatca gggcctatgg cgtcggttat aattaatcga aagtccctgg acttgaaggt
    17461 ggagatgcct agagacttcc atgcaatgag cggggaggag gccctcaccg ccctgggcac
    17521 gaggaccgag gggctttcga ccgcggaagc ccgggagcgg ctggcgcgct acggccccaa
    17581 cgagcttggc gaggggcgcg ggccctcccc c
//
LOCUS       DAOSYN010000007        17128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2774_length_17128_cov_4.293914, whole genome shotgun sequence.
ACCESSION   DAOSYN010000007 DAOSYN010000000
VERSION     DAOSYN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17128
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2774_length_17128_cov_4.293914"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /gene="glyS"
                     /locus_tag="PLJ11_00680"
     CDS             complement(<1..687)
                     /gene="glyS"
                     /locus_tag="PLJ11_00680"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOO03220.1"
                     /translation="MKEVSSGEMLSLCKRRGFIYPAYEIYGGIAGLYDYGPLGTALKG
                     NIADLWRRTYVLQEGFVEIDGDTIGPEAVFKASGHVDNFADLSVTCKACGESFRADHL
                     AKGLHPNPDSLSIDELQELLRANGVRCPCGGEFADVEEFNLMFKTKIGPGAGRTGYLR
                     PETAQSIFVNYINLYRHCRERLPFGCIQVGRGYRNEISPRQGVIRLREFNMMEAELFV
                     DPEDKSWPRYG"
     gene            complement(693..1541)
                     /locus_tag="PLJ11_00685"
     CDS             complement(693..1541)
                     /locus_tag="PLJ11_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO03221.1"
                     /translation="MATVGDVMTSNPIVAQVPGSRQEVLKIMVQHNLTGMPVVRRSDG
                     SLAGMITRQDIFDHPEEEQAAMIMRREHPVISPSASVEEAAKLLVEGTIRHLPVVDGN
                     RLVGILTPTDLLCIVEKRGIDLPVEKVIRSPCVPIYQEAPLAVASVTLRVSRSSALPV
                     LDEHGRLTGIITDRDIFNKSYTEGAEAVSAMGLNEEEGWSWEGLRNVMRLWYEVSKIE
                     LPGVPIKEIMVRDPTTVFKKTAISEAARIMRKNDFGQLPVRDNKDRLLAMIYDHDIIS
                     VLVKEQ"
     gene            complement(1542..1985)
                     /locus_tag="PLJ11_00690"
     CDS             complement(1542..1985)
                     /locus_tag="PLJ11_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO03222.1"
                     /translation="MVLFKKILIPTDGSEYTKAAVRMGLEIAKASEAEVTALYVVDQT
                     SFINFPTDSTIISVYTLLEKEGEEALEYVKQEAERLGVKVTPRIEEGSPSRKIVELSS
                     EHDLIVMGTLGRTGFSKLLLGSVAERVVRFAKCPVLVVRATGEEH"
     gene            complement(1990..3051)
                     /locus_tag="PLJ11_00695"
     CDS             complement(1990..3051)
                     /locus_tag="PLJ11_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO03223.1"
                     /translation="MRYLSGLVLGQNGFFPGHVGIEDGMVAEIGRGRKGSAYARGLIV
                     PTFVNAHTHIADYIVPVDPAMTLEELVAPPKGLKHRMLEQARPEDLIRGMLTMKRFMA
                     RRGVSHFLDFREGGYDGSEYLRRLDGEGAEAVIMGRPKLLEFCRSGTRKLLKVADGVA
                     VSSITDWEYSELEKLSKEVRAQGRPFALHASERVREDIDKVLALRPSHLVHMTEATDA
                     DMREVAEAGIPVVVCPRSNLFFGKVPPLGRMLAAGLTVALGTDNAMVCLPDMLVEMEV
                     AARILRAQGRNGAKDALDMAMNGRKLLNRQESFSIEPGVRCELMVLRHRGGDPVSDLV
                     LRSASDAPELVCIGKNCWR"
     gene            3190..3612
                     /locus_tag="PLJ11_00700"
     CDS             3190..3612
                     /locus_tag="PLJ11_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO03224.1"
                     /translation="MEKELRVLVAVDGSVHGEKALRKATLLALSSPSYEMAVVYVVEG
                     GGFATLTAEEDLGEMEERGRQVLRSSLDVLASEGVTATTVLLHGPPVTRILEFAETFR
                     PNLIVTGSKGMGAAKGPPVGSVASGLSRRASVSVLIVR"
     gene            3682..3849
                     /locus_tag="PLJ11_00705"
     CDS             3682..3849
                     /locus_tag="PLJ11_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO03225.1"
                     /translation="MAVKVNADECVGCGLCEDACPNGAITVDDIAVIDAVKCTECGTC
                     VDECPNSALSK"
     gene            complement(3929..4420)
                     /locus_tag="PLJ11_00710"
     CDS             complement(3929..4420)
                     /locus_tag="PLJ11_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HOO03226.1"
                     /translation="MDERKELEGALREWLAGAGTVVVAGIGNPIRRDDHAGVRVVEGL
                     RGAVSPRVHLIECETVPESFVDELVEVSPTHVLLIDAALLGLPPGTVDLYDVEEVAAT
                     AGIATHALPLRVLCEYVTLLTGARIALLLIEPEDTGFGEGMTPGLEAATRRTVGMLTA
                     LLP"
     gene            complement(4465..5916)
                     /locus_tag="PLJ11_00715"
     CDS             complement(4465..5916)
                     /locus_tag="PLJ11_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOO03227.1"
                     /translation="MKEINIDPITRLEGHGSISIFLNDEGEVENAYLKIPELRGFEAF
                     CVGRPAELMPILTTRICGVCPVAHHLAAVKTLDACFNVEPPPAARKLRELQYMGYICY
                     DHILHFYYLGGPDFIVGPDAPPEKRNILGVIEKAGMDVAKDVIRHRAYGQRITEIIGG
                     KATHPVSGLPGGVSKPLDEEGRRAILDMARSLVGFAQFSLATFHELVLGNPGYMDLIR
                     SDMFTQRTYYMGMVDARNRVSFCDGKVRVVDPAGSEFAKFDAQDYEEHIEERSEEWTY
                     SKFPFLRKVGWKGFTDGKDNGAYRVGPLGRLNAAEGMATPLANKEYQELFSLLGRPVH
                     STLAFHWARLIELLYAAERAVELASDPEITSPDVRNMPGEPGRGVGIVEAARGTLVHD
                     YQLNSDALVERVNMIVATTNNYPSICMSVRDAAKGLIHGGKVDERVLNKVEMAFRAYD
                     PCFGCATHFAVGQMPLDVKVYDHRKGLLCQLGR"
     gene            complement(5913..6875)
                     /locus_tag="PLJ11_00720"
     CDS             complement(5913..6875)
                     /locus_tag="PLJ11_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOO03228.1"
                     /translation="MAFYWAASCGGCEIAVLDTNEKVLELAEHADIVFWPVAMDIKYK
                     DVEAFPDGYVDVTFFNGSVRNSEQEHMAKVLRAKSKILVAFGACANQGSVPGLANLWS
                     ADELLDHVYLEVSGGRHNGSAVPRPEAEVREGTLRLPVLYDTVKTLDQTVAVDYYLPG
                     CPPPVELIKRAIDAILQGTLPPRGSVLAPLTAVCAECPRKREDKRITAIHRVHEVVPA
                     PERCLMEQGIVCMGMATRGGCGAQCLKVDMPCTGCGGPAPNRPDMGTGMLTALASILH
                     LDKEPGTYTEEEVMELMAQIKDPVGLFYMYSLPASILKRKVMKR"
     gene            complement(6887..8323)
                     /gene="gltA"
                     /locus_tag="PLJ11_00725"
     CDS             complement(6887..8323)
                     /gene="gltA"
                     /locus_tag="PLJ11_00725"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOO03229.1"
                     /translation="MAGDEPSRRRNDRALDEDVPMPRQDPKERVKNFKEVALGYTEEM
                     AVAEARRCLKCKSPQCQKGCPVEVPIPQFIDCIVQGRYEEGIALIKSKNALPAVCGRV
                     CPQEQQCQMNCVRGKRGDPVNIGRLERFLADWEREHGVRVPERPAATGRRVAIIGAGP
                     SGLTAASDLARLGHGVTLFEALHVAGGVLMYGIPEFRLPKEIVQREVEYVQSLGVDLR
                     LGNVIGRIATIPELLEQGYDAVYIASGAGLPQWTGCPGENLGGIYSANEFLIRANLMK
                     AYRFPETDTPLRIGKHVVVIGGGNVAMDCARISMRLGAKVCLNYRRSRNELPARREEI
                     ENAEEEGLTCNFLNAPAEFYGDEKGWVRAMRCTCMELTEPDERGRTGVRPIACSDFEM
                     ETDTVIVAIGQTPNPIIQRTTEGLRTNPRNGTIIVDEDGRTNLEGVFAGGDVATGAAT
                     VISAMGAGKRTARAIHEYLTTGPRGDRT"
     gene            complement(8310..9149)
                     /locus_tag="PLJ11_00730"
     CDS             complement(8310..9149)
                     /locus_tag="PLJ11_00730"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOO03230.1"
                     /translation="MYRIVAKERLAYKHTLFTVEAPDIAAKARPGQFLIVVPKEGGER
                     VPLSLCAYDQEAGTVSFAFHEVGRTTKELGTLQEGEGLFNVTGPLGNPSEVRRYGRVL
                     CIGGGIMIAPALLQARALKEAGNEVITILACRSAEFLFKREEAERVSDRVLIASDDGT
                     VGERGLDFLTDLLAKDRIDRCTVIGPVVMMKRVCELTRPFAVPTIVSLTPIMIDGMGM
                     CGACRVTVGDRMLFGCVDGPEFDGHRTNFDELIKRQRSMLPEERLSSMLWEHKGGCAC
                     GRR"
     gene            complement(9150..10352)
                     /locus_tag="PLJ11_00735"
     CDS             complement(9150..10352)
                     /locus_tag="PLJ11_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOO03231.1"
                     /translation="MNDNCIAAVKVNQDLCSRCKVCYSTCPFEAIRQRPDDGRIEIDI
                     QKCQVCGICYSACPVSAIEMAYYDHAGLLEHVRAACPGAETLVMMCRGNSPSSGEVED
                     ILADHGLDVKGYVPMRVPCAGRVPTDLIFNALASGIRNIVSIQCQDGFCRMKEGTRIE
                     TRRLLLSKAVLRQLGYPEDAVQVVKYSRKAVWTGHECVGCDKCVFVCPYGAIEAGAIS
                     SPRIDPGKCVGCGACQVVCPHNAIQVKGYEFYSVLRSYEEAAERMRKTGRPSVLVFSC
                     QWSEFTALDDPEGVLGGRNAMLLEVPCFKGLDPVHVLTALQSGFDGVMGVVCAPEDCK
                     LKNGRDAAERQLEVLLIALKRLGLSERFELHELSPRCKGEFAQAFDAFYARVSAMPRG
                     GACRREGD"
     gene            10687..12048
                     /gene="gdhA"
                     /locus_tag="PLJ11_00740"
     CDS             10687..12048
                     /gene="gdhA"
                     /locus_tag="PLJ11_00740"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006929.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOO03232.1"
                     /translation="MACHRNASAMLTKELMERIVAKNPGEKEFHQAVHEVFESLEPVL
                     KQHPEYLKAKILDRIVEPDRQVMFRVTWVDDKGEVQVNRGYRVQFNSAIGPYKGGLRF
                     HPSVNLGILKFLGFEQVFKNALTGLPMGGAKGGADFDPKGKSDAEVMRFCQSFMSELY
                     RHIGPNTDVPAGDIGVGAREIGYLFGQYRRLNNRFEAVLTGKGINWGGSLVRPEATGY
                     GCAYFCEEMLRTKKQDLKGKTVAISGSGNVAQYAVQKVTQLGGKVVTMSDSDGTVYDP
                     DGIDAKKWEFVMDLKNVRRGRISEYAQRFGCEYWPGERAWKAKCDIAMPCATQNELEK
                     DDAKRLIDNGVIAVSEGANMPCTPEAVELFLANGVLFAPGKAANAGGVATSGLEMCQN
                     HIGISWSAEEVDRQLHQIMVNIHRNVADSAKKYGSPGNYVVGANITGFVKVASSMLDQ
                     GVL"
     gene            12339..12887
                     /gene="thpR"
                     /locus_tag="PLJ11_00745"
     CDS             12339..12887
                     /gene="thpR"
                     /locus_tag="PLJ11_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOO03233.1"
                     /translation="MSLRAFVAVDVPPSQELERAIEGLRGFGRALKPVLPANIHITLK
                     FLGEIDEGTVPGIEAAMRRAVEGVRPFALRLVGIGAFPSTHSPRVIWTGIEGAEPLAK
                     MAAALEEGCEPLGFPRERRPFSPHLTLARVREGQRPDLHAFLEMYRGRDLGSFQVDRV
                     RLKRSVLTPSGPIYSDLLEIPL"
     gene            complement(12884..14275)
                     /locus_tag="PLJ11_00750"
     CDS             complement(12884..14275)
                     /locus_tag="PLJ11_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOO03234.1"
                     /translation="MFKKVLIANRGEIAIRVINTCKRMGVLTVGIYTPSDKHCKHVSL
                     ADKSVELRTDGNSIGYLDIDAIIDVANRMKVDAIHPGYGFLSEKPEFSERCEEEGIAF
                     LGPSHRAMRLLGGKLSAREFMSKVGVPIMPGTLEPLETPEEAIRQANKIGYPVLLKAS
                     GGGGGRGMRLVHSDEEMENALTSAKSEADKAFKNPTIYLERALMRARHIEFQVVGDSR
                     GNAYCLGERECSVQRRHQKIIEETPSCAVGPELRQRMSELCVAAVKRAGYTSLGTFEF
                     LMSAEGELFFLEANTRIQVEHPITEQVTGLDLVELQLKIGSDIPVEDVLSKVRPKGHA
                     MEFRINAENPFNNFFPAPGRIEKYVEPAGDHVRVDSHAYEGYVVPTEFDNLLAKLVIS
                     GKTRNEVLSRAQEALDRYTVTGIKTTIPYHRLVVRNPDFRAGDYSIDFVKEHPPQDLL
                     KTTEFSLFESIGR"
     gene            14527..15006
                     /locus_tag="PLJ11_00755"
     CDS             14527..15006
                     /locus_tag="PLJ11_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOO03235.1"
                     /translation="MRFSRKRISGDREGGIEGLPLQLMIMVIIAGLGTAVLVGWMGGL
                     SAPQAISSVGASPSEVLLTDTDGDGTFENESVTITIVVRDQNGDAVEGASVVLDGCNV
                     RTADGALVYGTTGADGKATFTGLTVSSSGNGIGFITVTAAKGGMGTDSSLQIPVISR"
     gene            15011..15454
                     /locus_tag="PLJ11_00760"
     CDS             15011..15454
                     /locus_tag="PLJ11_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03236.1"
                     /translation="MRRDERGIEGLPLRLLIVALLISLTLPAMLSVLDGTTAGMAERK
                     AAEVAEDIARTVEEMAAAGPGNVRVVDVPVDLPPNVVLNIGGGNGTAASARIAWSAGG
                     DEGARYLRGVCVLSGDNAPVGLTPGDRMRLECPPGQWGVVTVAKL"
     gene            15451..15870
                     /locus_tag="PLJ11_00765"
     CDS             15451..15870
                     /locus_tag="PLJ11_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03237.1"
                     /translation="MMEFFLSKVWAFLVAFALLGVLLQGVQTTAHADRSEALRELAAD
                     LEELFADLVAAGPGLERTVHLEGMLPSTATLLVQKGYAVLCDEGMEVPFSAPFAQLLM
                     ETASGALEETEALTFGPSDSFLVINEDRGTTIVALSP"
     gene            complement(15860..17068)
                     /locus_tag="PLJ11_00770"
     CDS             complement(15860..17068)
                     /locus_tag="PLJ11_00770"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOO03238.1"
                     /translation="MDVNRVRKDFPLYQQASPPIYLDSACQSLRPRQVIEAMDEYYCA
                     FPACGGRSVHRLATEVSVRMDRAREEIAAFVGARDPECVVFTKSATESLNTVAKGLRL
                     RKGERVITTDMEHNSNHVPWVLLARERGIRRGIVPFSALEQGDLEPLKEELEKGARLV
                     SVVHTNNVDGSTIPLEAVVELAHDRGALVMADGCQTAPHSPLALDRTDVDLYAWSMHK
                     MLGPSGMGALYGKREVLEGIEPLVTGGGAVAITSYDSAELLPVPERFEAGLQNFAGII
                     GSGAAVRYLREVGMEEIVEHEHRLNALATEGLSAIEGLSILGPSDPRRRGGILSFNLR
                     GMLPHDVAMILDEIASVLIRSGMHCVHPYFAARGMEGCARASLYLYNTEEEMRQFVEA
                     VGYIAERFMG"
BASE COUNT         2858 a         5770 c         5370 g         3130 t
ORIGIN      
        1 gccgtatcgg ggccaggact tgtcctctgg atccacgaac agctcggcct ccatcatgtt
       61 gaactccctg agccggatca ccccctgtct cggggagatc tcgttgcgat agcccctgcc
      121 gacctggatg cagccgaacg ggagcctctc gcggcagtgc cgatagaggt tgatgtagtt
      181 tacgaagatg gactgcgcgg tctccggccg aaggtacccc gtccttccag cccccggacc
      241 gatcttcgtc ttgaacatca ggttgaactc ctccacatcg gcgaactccc caccgcacgg
      301 gcaccggacg ccattggccc ggagcagctc ctggagctcg tcgatggaca gggaatcggg
      361 attggggtgc agccccttcg ccaggtggtc cgcccggaac gattccccgc aggccttgca
      421 ggtcacggag aggtcggcga agttgtccac gtgcccggag gccttgaaca ccgcctcggg
      481 cccgatggtg tccccgtcga tctccacaaa cccctcctgt aggacgtagg tcctgcgcca
      541 aaggtcggcg atgttgccct tcagggcggt ccccaggggg ccataatcat acaggccggc
      601 aatgccgccg tagatctcat aggcagggta gatgaaccca cgcctcttgc atagggagag
      661 catctccccg gagctgacct ccttcatctg gttcattgct cctttacaag gacggaaatg
      721 atgtcgtgat cgtagatcat ggccagcaac cggtccttgt tgtcccggac gggcagctgg
      781 ccgaagtcgt tcttccgcat tatgcgggcc gcctcggaga tggcggtctt cttgaacacg
      841 gtggtcgggt cgcgcaccat gatctccttg atcggcacgc cggggagttc gatcttggaa
      901 acctcgtacc agagcctcat gacgttgcgg agcccttccc aggaccagcc ctcctcctcg
      961 ttcaggccca tggcggagac cgcctcggcc ccctccgtgt agctcttgtt gaatatgtcg
     1021 cggtccgtga ttatgccggt gaggcgccca tgctcgtcca gcaccggcag ggcggacgat
     1081 ctggagaccc gcagggtgac cgaggccacc gctagcgggg cttcctggta gatcggcacg
     1141 cagggcgagc ggatcacctt ctccaccggg aggtcgatgc ccctcttctc cacgatgcag
     1201 agcaggtcgg tgggcgtcag aatgccgacg agccggttcc cgtccaccac aggcagatgg
     1261 cggatcgtgc cctccaccaa cagcttcgcc gcctcctcca cggaggccga aggggatatc
     1321 acgggatgct cgcgccgcat gatcatcgcc gcctgctcct cctccggatg gtcgaagatg
     1381 tcctggcggg tgatcatgcc cgcgagggag ccatcgctcc tgcggacgac gggcattccg
     1441 gtcaggttgt gctggaccat tatcttcagc acctcctgcc gggacccagg cacctgggcg
     1501 acgatagggt tggacgtcat cacgtccccg acggtcgcca tctagtgctc ctcccccgtg
     1561 gccctcacta caaggacggg gcacttggcg aagcgcacca cgcgctcggc cacgctcccc
     1621 aagaggagct tggagaagcc cgtgcggcca agggtgccca tgacgatcag gtcgtgctcg
     1681 gaggacagtt ccacgatctt gcgggacggg gaaccctcct ctatgcgggg cgtcaccttc
     1741 acgccgagcc tctcggcctc ctgcttaacg tattccaagg cctcctcgcc ctccttctcc
     1801 aggagggtgt agacgctgat gatggtggag tcggtgggaa agttgatgaa ggacgtctgg
     1861 tcgaccacgt acagggcggt gacctccgcc tccgacgcct tggcgatctc caaccccatc
     1921 ctcactgcgg ccttggtata ttcgctccca tcggtcggga tcaatatctt cttgaacagg
     1981 accatccttt catctccagc agttcttccc tatacatacg agctcaggcg cgtccgaagc
     2041 gctgcgcagt acgaggtcgg agacagggtc ccctccccgg tgcctgagca ccatcagttc
     2101 gcaacgtaca cccggttcta tactaaacga ttcctggcga tttaataatt ttcttccgtt
     2161 cattgccata tccagcgcgt ccttcgcccc gttcctgccc tgcgcccgga ggattcgggc
     2221 cgctacctcc atctccacca gcatatcggg caggcagacc atggcgttgt ccgtccccag
     2281 agcgacggtg agcccggcgg cgagcatcct tcccagcggg ggcacctttc cgaagaacag
     2341 gttcgagcgg gggcagacca ccaccggaat ccccgcctcg gcgacctcgc gcatgtccgc
     2401 gtccgtcgcc tccgtcatgt gcacgaggtg cgaaggcctc agggccagga ccttgtcgat
     2461 gtcctcccgc accctctcgg aggcgtggag ggcgaacggc ctcccctggg cgcgcacctc
     2521 cttggagagc ttctcgagct ccgagtactc ccaatcggtg atggaggaga cggccacgcc
     2581 gtcggccacc ttcagcagct tccttgtccc ggaccggcag aactccagga gcttcgggcg
     2641 gcccatgatg accgcctcag ccccctcgcc gtccaggcgc ctcaggtatt cggagccgtc
     2701 gtacccgccc tccctgaagt caaggaagtg ggagaccccc cgccgggcca tgaaccgctt
     2761 catggtgagc atcccacgga tgaggtcctc cggcctcgcc tgctccagca tccgatgctt
     2821 cagccccttg ggcggggcga caagctcctc cagggtcatg gccggatcga cggggacgat
     2881 gtagtcggcg atgtgggtgt gcgcgttcac gaacgtcggg acgatgagcc cccgggcgta
     2941 ggcgctcccc ttccgcccgc gcccgatctc ggccaccatc ccgtcctcga tgcccacgtg
     3001 cccggggaag aagccgttct ggcccaggac caggccggag aggtaccgca taccagggcg
     3061 atcgccctga ggctatatca accgcatggt gataaaggcc gacctgtcga tggcccagag
     3121 cgcaacgctc aataggggcg cggaggatgg gcatacggac ccaagagccc gggaggaaca
     3181 gggttggtca tggagaagga gctaagggtg ctggtggcgg tcgacggttc ggtccacggg
     3241 gagaaggcgc tccggaaggc aaccctgctc gccctctcct ccccctccta tgagatggcc
     3301 gtcgtgtacg tcgtcgaggg cggcggattc gccaccctga ccgcggagga ggacctgggg
     3361 gaaatggagg agcggggaag gcaggtcctg cgctcttccc tggatgtcct cgcctccgag
     3421 ggcgtgacgg ccacgacggt cctcctccat ggcccgccgg tgaccaggat cctggagttc
     3481 gccgagacgt tccggcccaa cctgatcgtc accgggagca agggcatggg ggcggccaag
     3541 ggccctcccg tcgggtccgt cgcctcgggc ctctccagaa gggccagcgt ctccgtcctc
     3601 atcgttagat gagttgctat ccagaatggg caaagcatat atacggcaac ttttcttgca
     3661 ccttctcata ggtgctttac aatggctgta aaggttaatg cggacgagtg tgttggttgc
     3721 ggactttgcg aggatgcctg ccctaacggg gccataaccg ttgacgatat agccgtcatc
     3781 gacgccgtga agtgcaccga gtgcggcacc tgcgtggacg agtgcccgaa cagcgcgctc
     3841 tccaagtaag cgcacggtgc cccttccacc ggacaaacct ctttgctaca ggtttttcag
     3901 ggtcgccctg cgcacccccc atccgttctc atggtagcaa agccgtcagc attcctaccg
     3961 tgcgtcgggt cgccgcctca agcccggggg tcatgccctc cccgaagccg gtgtcctccg
     4021 gctcgatcaa cagaagggcg atcctcgccc cggtgagcag ggtgacgtac tcgcagagca
     4081 cgcgcagggg aagggcatgc gtcgctatgc ccgcggtggc cgcgacctcc tccacgtcat
     4141 agaggtccac ggtcccgggg gggaggccga ggagggccgc atctataagg agcacgtgcg
     4201 tcggggagac ctccactagc tcgtccacga agctctccgg caccgtctcg cactcgataa
     4261 ggtgcacgcg cggcgagacg gcgcccctca acccctcgac gaccctcacc cctgcgtggt
     4321 cgtcccggcg gatgggattc cctatcccgg ccacaaccac cgttcccgcc ccggcgagcc
     4381 attccctgag cgcgccctcc agttctttcc tttcgtccat ccgcatcccc taccatcggg
     4441 ccggagggcg aaggggcctc ccggtcaacg gcccagctgg catagcagcc ctttgcgatg
     4501 gtcgtacacc ttcacgtcca ggggcatctg ccccacggcg aagtgcgtgg cgcagccgaa
     4561 gcaggggtca taggcgcgga aggccatctc caccttgttc agcacccgtt cgtcgacctt
     4621 cccgccgtgg atcagccctt tggcggcatc ccggacactc atgcagatgg acgggtagtt
     4681 gttggtggtc gccacgatca tgttgacgcg ctcgaccagc gcgtcgctgt tgagctgata
     4741 gtcatgcacc agggtgcccc gggccgcttc aacgattccg accccgcgcc caggctcccc
     4801 gggcatattc cgcacgtccg gagaggtgat ctccgggtcg ctggcgagct ccaccgccct
     4861 ctcggccgcg tacagcagct cgatgagcct cgcccaatgg aaggccaggg tgctgtgcac
     4921 cggcctacct aaaagggaga atagctcctg gtactccttg tttgccagag gagtggccat
     4981 cccctccgcg gcgttcaacc ggccgagcgg gccgaccctg taggcgccgt tgtccttccc
     5041 gtcggtgaag cccttccacc ccaccttcct gaggaagggg aacttggagt aggtccattc
     5101 ctccgaccgc tcctcgatgt gctcctcata gtcctgggca tcgaacttgg cgaactccga
     5161 gccggccggg tccacgaccc tgaccttccc atcgcagaag ctcaccctgt tccgggcgtc
     5221 caccatcccc atgtagtagg tcctctgggt gaacatgtcg ctgcggatga ggtccatgta
     5281 cccggggttg ccgagcacca gctcatggaa cgtagcgagg gagaactggg cgaagcccac
     5341 aagggacctg gccatgtcca gtatggccct gcgcccctcc tcgtccagcg gtttgctcac
     5401 cccccctgga agcccgctga cggggtgcgt ggccttcccc ccgatgatct ccgtgatccg
     5461 ctgcccgtac gcccgatgcc ggatgacgtc cttggccacg tccatcccag ccttctcgat
     5521 cacgcccagg atgttcctct tctccggggg cgcgtccggc cccacgatga agtccggccc
     5581 gccgaggtag tagaagtgca ggatgtggtc gtagcagatg taccccatgt actggagctc
     5641 gcgcagcttc ctcgccgcag gcgggggttc gacattgaag cacgcgtcca acgtcttgac
     5701 ggccgccagg tgatgggcga ccgggcagac gccgcagatg cgcgtcgtca ggatgggcat
     5761 cagctccgcc ggccggccca cgcagaacgc ctcgaacccc ctgagctcgg ggatcttcag
     5821 gtaggcgttc tccacctctc cctcgtcgtt gaggaagatg gatatgctgc catgcccctc
     5881 cagccgggtg atcgggtcta tgttgatctc cttcatcgct tcatcacctt cctcttgagg
     5941 atcgacgcgg gcaggctgta catgtagaag aggccgaccg ggtccttgat ctgggccatg
     6001 agttccatga cctcctcctc cgtgtacgtg cccggctcct tgtccaggtg gaggatggat
     6061 gccagggcgg tcagcatgcc cgtccccatg tccggccggt tcggggcggg ccctccgcag
     6121 ccggtgcagg gcatgtccac cttcaggcac tgcgccccgc accctccccg ggtggccatg
     6181 cccatgcaga ctatcccctg ctccataagg cagcgctccg gggcgggcac gacctcgtgc
     6241 acccgatgga tggccgtgat ccgcttgtcc tcccgcttgc gcgggcattc cgcgcacacg
     6301 gcggtgaggg gggcgagcac ggacccccgt gggggcagtg tcccctgcag tatggcatcg
     6361 atcgcccgct tgatcagctc caccggcgga gggcatccag gaagataata gtccaccgcc
     6421 acggtctggt ccagcgtctt gacggtgtcg tagaggaccg ggaggcggag cgtgccctcc
     6481 ctcacctcgg cctcgggcct ggggacggcc gagccattgt gccttccccc gctgacctcc
     6541 aggtacacgt ggtccaggag ctcgtccgcg gaccataggt tcgcgaggcc ggggacggag
     6601 ccctggttgg cgcaggcccc gaatgcgacc aggatcttcg acttcgcccg caggaccttc
     6661 gccatgtgtt cctgctcgct gttccgcacg gagccgttga agaaggtcac gtccacgtat
     6721 ccatcgggga acgcctcgac gtccttgtac ttgatgtcca tggccaccgg ccagaacacg
     6781 atgtccgcgt gctccgccag ctccaggacc ttctcgttcg tgtccaggac cgctatctcg
     6841 cagccgccgc agctggcggc ccaatagaac gccaccttca gcttctctat gtacggtccc
     6901 cccttggtcc ggtcgttagg tattcatgga tagccctggc ggtcctcttc ccggcgccca
     6961 tggcgctgat gaccgtggcc gcccccgtgg ccacgtcccc gccggcaaag accccctcca
     7021 ggttcgtccg gccgtcctcg tccacgatga tcgtcccgtt ccggggattg gtcctgagcc
     7081 cctcggtcgt cctctggatg atgggattgg gcgtctgccc tatcgccacg atcacggtgt
     7141 ccgtctccat ctcgaagtcc gagcaggcga tgggcctcac ccccgtcctc cccctctcgt
     7201 ccggttcggt gagctccatg caggtgcagc gcatcgcccg cacccaccct ttctcgtccc
     7261 cgtagaactc cgccggggcg ttgaggaagt tgcaggtcag cccctcctcc tccgcgttct
     7321 cgatctcctc gcgccgggcg ggcagctcgt tccttgagcg ccggtagttc aggcacacct
     7381 tcgcccccag ccgcatggag atgcgcgcgc agtccatggc cacgttcccc ccgccgatca
     7441 ccaccacatg cttgcctatg cgaaggggcg tgtcggtctc cggaaagcga taggccttca
     7501 tcaggttggc cctgatcagg aactcgttgg ccgaatagat ccctccgaga ttctctcccg
     7561 ggcaccccgt ccattggggc aggcccgccc cgctcgctat gtataccgca tcatacccct
     7621 gttccagcag ctcggggatg gtggcgatgc gccctatgac gttcccgagg cggaggtcta
     7681 cgccaaggct ctggacgtac tccacctccc tctgcacgat ctccttcggc agccggaact
     7741 ccggaatgcc gtacatgagc acccccccag ccacgtgaag ggcctcgaac agcgtgaccc
     7801 catggcccag ccgggcgagg tcggaggcgg cggtaagccc ggagggcccg gccccgatga
     7861 tggccaccct cctgcccgtg gccgcggggc gttccggcac acgcacgcca tgctcccgct
     7921 cccagtccgc caggaagcgc tccaggcgac cgatgttgac cgggtcgccc ctcttgcccc
     7981 ggacgcagtt catctggcat tgctgctcct gcgggcacac ccggccgcac accgcgggga
     8041 gggcgttctt ggacttgatg agggctatcc cctcctcata cctcccctgg acgatgcagt
     8101 cgatgaactg ggggatgggc acctccaccg ggcacccctt ctggcactgc gggctcttgc
     8161 acttgaggca gcgccgggcc tccgccaccg ccatctcctc cgtgtacccg agggcgacct
     8221 ccttgaagtt cttgacccgc tccttggggt cctggcgcgg catggggacg tcctcgtcca
     8281 atgccctgtc gttcctccgc cgggagggct catctccggc cacaggcgca ccctcccttg
     8341 tgctcccaga gcatggagct gaggcgttcc tcgggcagca tgctgcgctg ccgcttgatc
     8401 agctcgtcga agttggtcct atggccgtcg aactcgggcc cgtccacgca tccgaagagc
     8461 atcctgtccc cgacggtcac ccggcacgcc ccgcacatgc ccatgccgtc gatcatgatc
     8521 ggggtcaggg agacgatggt ggggacggcg aacgggcggg tgagctcgca gaccctcttc
     8581 atcatgacca ccgggccgat cacggtgcaa cggtcgatcc ggtccttagc aagaaggtcg
     8641 gtcaggaagt ccagtcccct ctcccccacg gtcccgtcgt ccgaggcgat gagcacccgg
     8701 tcgctgacgc gctcggcctc ctccctcttg aacaggaact ccgcgctgcg gcaggcgagg
     8761 atggtgatga cctcgttccc cgcctccttc agcgccctgg cctgcagcag ggcgggggcg
     8821 atcatgatgc ccccgccgat gcacagcacc ctgccgtacc tccggacctc ggaagggttg
     8881 ccaagggggc cggtgacgtt gaacagcccc tccccctcct ggagcgtccc gagctccttc
     8941 gtggtcctcc cgacctcgtg gaaggcgaac gagaccgtgc ccgcctcctg gtcgtaggcg
     9001 cagaggctga ggggcaccct ttcccctccc tccttcggta ccacgatcag gaactgcccc
     9061 ggccttgcct tggcggcgat gtcgggggcc tccacggtga acaaggtgtg cttgtacgcc
     9121 agacgctcct ttgccactat ccggtacatt cagtccccct ccctgcggca tgccccgccg
     9181 cggggcatgg ccgagacccg ggcgtagaag gcgtcgaacg cctgggcgaa ctcccccttg
     9241 catcgggggg atagctcatg cagctcgaag cgctccgaaa gccccagcct cttgagggcg
     9301 atcaggagca cctcgagctg cctctccgcg gcgtccctgc cgttcttcag cttgcagtcc
     9361 tcgggtgcgc acactacccc catcacccca tcgaacccgc tctggagggc ggtgagcacg
     9421 tgcacggggt ccagcccctt gaagcagggg acctccagta gcatggcgtt ccttcccccc
     9481 agcaccccct cagggtcgtc cagcgcggtg aactcggacc actggcagct gaacaccagg
     9541 accgatggcc gtccggtctt gcgcatccgc tccgccgcct cctcgtagga gcgcagcacg
     9601 gaatagaact cgtacccctt cacctggatg gcgttgtgcg ggcagacaac ctggcacgcc
     9661 ccgcacccca cgcacttccc cggatcgatg cggggggacg agatcgcccc ggcctcgatg
     9721 gccccgtacg ggcagacgaa gacgcatttg tcgcagccga cgcactcgtg gccggtccac
     9781 accgccttcc tcgagtactt gacgacctgc accgcgtcct ccgggtaccc gagctgccgc
     9841 agcaccgcct tgctgaggag cagcctcctg gtctcgatcc gggtgccctc cttcatgcgg
     9901 cagaagccgt cctggcattg gatcgacacg atgttcctga tcccggaggc cagggcattg
     9961 aagatgaggt ccgtgggcac ccgccccgcg cacgggacgc gcatggggac gtacccctta
    10021 acgtccaggc catggtccgc caggatatcc tccacctcgc cgctgctggg ggaattgccc
    10081 cggcacatca tgaccagcgt ctccgccccg ggacaggcgg cccgcacgtg ctccaggagg
    10141 ccggcgtggt cgtagtaggc catctcgatg gccgagaccg ggcaggcgct atagcatatc
    10201 ccgcatacct ggcacttctg gatgtcgatc tcgatccgcc cgtcgtccgg gcgctggcgg
    10261 atcgcctcga acggacaggt ggagtagcag accttgcacc ggctgcaaag gtcctggttc
    10321 accttcacgg cggctatgca gttgtcattc atcgatggtc ccccagcggt cccgttccct
    10381 gcgctctcct gtgctcaggg catgccccga tcggagggcc gttcatggac ggaggggcat
    10441 tcaggacctt gatggcccat gccatcggtc tcttctaacg cataatggcc ttattattta
    10501 atgcatatgc ccgatcgttc gtacccgccc agttccccca ggccgaaggg caggttcttc
    10561 ttcaatgggc ggacatctgc gcacggtcct tgggaaacat cggcaatgag gccggtccca
    10621 accctgttct tcgggccttc aacacagata tataagaaaa gtgcaatcgc ccaccgtcgc
    10681 caagcgatgg cttgccacag gaacgcatcc gctatgctca ccaaggaact gatggagagg
    10741 atcgtggcca agaaccccgg ggagaaggag ttccaccagg ccgtgcatga ggtcttcgaa
    10801 tcgctggaac cggtgctgaa gcagcatccc gagtacctta aggccaagat attggacagg
    10861 atagtcgagc cggaccgaca ggtcatgttc agggtcacat gggtggacga caaaggggag
    10921 gtccaggtca accgcggata cagggtgcag ttcaacagcg ccataggccc ctacaaggga
    10981 gggctgcggt tccatccatc ggtcaacctg ggcatcctga agttcctggg gttcgagcag
    11041 gtgttcaaga acgccctcac cggcctgccg atgggcgggg ccaagggcgg ggccgacttc
    11101 gatcccaagg gcaagagcga tgcggaggtc atgcgcttct gccagagctt catgagcgaa
    11161 ctgtaccgcc acatcggccc taacacggac gttcccgcgg gggacatagg cgtcggcgcc
    11221 cgggagatcg gttacctctt cgggcagtac aggaggttga acaaccgctt cgaggccgtg
    11281 ctcaccggca aggggatcaa ctggggcggg tccctggtcc gcccggaggc caccggctac
    11341 gggtgcgcct acttctgcga ggagatgcta aggaccaaga agcaggatct caagggaaag
    11401 acggtggcca tctccggctc cggcaacgtc gcccagtacg ccgtgcagaa ggtcacccag
    11461 ctggggggca aggtcgtcac catgtccgat tccgacggca ccgtctatga ccccgatggg
    11521 atagacgcca agaagtggga gttcgtgatg gacctcaaga acgtcaggcg gggacggata
    11581 agcgagtacg cgcagcggtt cggatgcgag tactggcccg gggaaagggc gtggaaggcc
    11641 aagtgcgaca tcgccatgcc ctgcgccacc cagaacgagc tggagaagga cgacgccaag
    11701 cggctgatcg acaatggcgt gatcgccgta tccgaagggg ccaacatgcc gtgcacgccg
    11761 gaggcggtgg agctgttcct cgccaacggc gtgctcttcg cgccggggaa ggcggccaac
    11821 gctggaggcg tggccacctc cggcctggag atgtgccaga accacatcgg catctcctgg
    11881 tccgccgagg aggtcgaccg gcagctgcat cagataatgg tgaacatcca caggaacgtg
    11941 gcggactccg ccaagaagta cggaagcccg ggcaactatg tggtcggcgc caatatcacc
    12001 ggctttgtga aggtggcttc ctcaatgctc gaccagggcg tcctctgagc cctccgggca
    12061 aggcgccggc ccccggcgtc ccgaggcccg ggccgctcaa cctttttatt cgcggggaac
    12121 gctcttaata tccaaagtga gggggtgtag aggctgacca tcgctggggg ccgggacagg
    12181 aggggcggga tcgccctatc cattgcatgg agggctgtga acatggtgca gcccgtccat
    12241 gcgcgggagg gcagggaagc ggcgccgagg gccgaagggg gacgaagggc cgagcttcca
    12301 ttggcggcct gggaacgctc cgatgcgctg gtgggggcat gagcctccgc gccttcgtgg
    12361 ccgtggacgt gcccccctcc caggagctgg agcgcgcgat cgaagggctc agggggttcg
    12421 ggcgggcgct caagccggtc ctcccggcca acatccacat caccctgaag ttcctggggg
    12481 agatcgatga gggcaccgtt ccagggatcg aggcggccat gcgcagggcc gtggagggcg
    12541 tacgcccctt cgcgcttcgg ctcgtcggca taggcgcgtt cccgagcacg cacagcccaa
    12601 gggtcatctg gacaggcatc gagggggcgg aaccgttggc gaagatggct gccgcgctgg
    12661 aggagggctg cgagcccttg ggcttcccaa gggagaggag gccgttcagc ccccatctca
    12721 cgctcgcgcg ggtgagggag gggcagaggc cggacctgca tgcgttcctg gagatgtaca
    12781 ggggacggga cctcggttcg ttccaggtcg acagggtaag gctgaagagg agcgtgctga
    12841 cgccctccgg gccgatctac tccgacttgc tggagatccc tctttagcgc ccgatgctct
    12901 cgaacaggga gaactcggtg gtcttcagca ggtcctgggg cgggtgctcc ttgacgaaat
    12961 cgatgctgta gtcgcccgcg cggaagtcgg ggttcctgac caccaggcgg tggtacggga
    13021 tcgtggtctt gatcccggtg accgtgtagc ggtccagggc ctcctgcgcc ctgctcagga
    13081 cctcgttcct ggtcttcccc gatatcacga gcttggcgag caggttgtcg aactccgtag
    13141 gaacgacata gccttcgtag gcgtgggagt ccacgcgcac gtggtccccg gccggttcca
    13201 catacttctc gatcctcccc ggggcgggga agaagttgtt aaatgggttc tccgcgttga
    13261 tcctgaactc catggcatgc cccttcggcc ttaccttgga caggacgtcc tccacgggaa
    13321 tatcggagcc gatcttgagc tgcagctcga ccaggtccaa cccggtcacc tgctcggtga
    13381 tgggatgctc gacctggatc ctggtgttgg cctcgaggaa gaacagctcc ccctcggcgg
    13441 acatgaggaa ctcgaacgtc cccagcgagg tgtagcccgc gcgcttgacc gcagccacgc
    13501 acagctcgga catcctctgc ctcagctcgg ggccgacggc gcaggacggc gtctcctcta
    13561 tgatcttctg gtggcggcgc tgcaccgagc attccctctc ccccaggcag taagcgttcc
    13621 cgcgggagtc gccgacgacc tggaactcga tgtgcctcgc cctcatcaac gccctctcca
    13681 agtatatggt ggggttcttg aaggccttgt ccgcctcgct cttggcggag gtcagggcgt
    13741 tctccatctc ctcgtcgctg tggaccagac gcatcccgcg ccctccccct ccccccgacg
    13801 ccttcagaag gacggggtac ccgatcttgt tggcctgcct gatcgcctcc tccggggtct
    13861 ccaaaggctc caaggtgccg ggcatgatgg ggaccccgac cttggacatg aactccctgg
    13921 cggacagctt tccccccagc agcctcatgg ccctgtggga cgggcctagg aacgctatcc
    13981 cctcctcctc gcaccgctcg gagaactccg gcttctcgga caggaagccg taccctggat
    14041 ggatggcgtc caccttcatc cggttggcca catcgatgat cgcgtcgatg tcaagatatc
    14101 cgatgctgtt gccgtcggtc cggagctcga cgctcttgtc cgccagcgac acatgcttgc
    14161 aatgcttgtc cgagggggtg tatatgccca cggtgagcac gcccatcctc ttgcaggtgt
    14221 tgatcacccg aatggcgatc tcccctctgt tcgctatcag caccttcttg aacatctcga
    14281 gccctcaggc gcatggcctc gcgcatgcga ctgcgtggca gggaatgtac cttcttttta
    14341 taataatttc gcctccccaa acttgatttc tttttgattt cggatggaat attcgtttta
    14401 cgacaagaat ataatctatt cttgccaaaa atcgttttta tccttgccga atgccccttc
    14461 aaaggtagtc tcattgtaag ggagagggcc ttgaaagcct gcgcccttcc tcattttgga
    14521 gatacaatga ggttctcaag gaaacggata agtggggaca gggagggcgg catcgaaggg
    14581 cttccccttc agctgatgat aatggtcatc atagccggac tgggcacggc cgtcctcgtg
    14641 ggatggatgg gcgggctgtc cgcgccgcag gcgatctcat cggtgggcgc cagcccgtcc
    14701 gaggtcctgc tgacggacac ggacggcgac ggcaccttcg agaacgagag cgtgaccatc
    14761 acgatcgtgg tgcgggacca gaacggggat gcggtcgagg gcgcctccgt ggtgctggac
    14821 ggatgcaacg tgcgcaccgc cgacggggcg ctcgtctacg ggacgaccgg ggcggacggc
    14881 aaggcgacgt tcacagggct gacggtctcg tcctccggca acggcatcgg cttcataacc
    14941 gtgaccgccg ccaagggagg gatgggcacg gactcctcgc tgcagatccc ggtcatctcc
    15001 aggtgagccc ttgcgccggg acgaaagggg gatagagggg ctgcccctgc ggctgctcat
    15061 agtggccctt ctgatctccc tcaccctgcc cgcgatgctc tctgtccttg atgggacgac
    15121 agcggggatg gccgagagga aggcggccga ggtggccgag gacatcgccc gcacggtcga
    15181 ggagatggcc gcggccggcc ccgggaacgt gcgcgtcgtc gatgttccgg tggaccttcc
    15241 tccgaacgtc gtgctcaaca tcggcggggg caacggcacc gcggcaagcg cccggatagc
    15301 ttggagcgcc ggcggggacg agggcgcccg gtacctccgc ggggtatgcg tgctctccgg
    15361 ggacaacgcg ccggtggggc tcacccccgg cgaccggatg cgcctggaat gcccccccgg
    15421 gcagtggggc gtggtgacgg tggccaagct atgatggagt tcttcctttc caaggtatgg
    15481 gccttcctgg tggcgttcgc cctcctgggc gtgctgctgc agggcgtgca gacgaccgca
    15541 catgcggaca ggtcggaggc gctgagggaa ctggccgccg acctcgagga gctgttcgcg
    15601 gaccttgtcg ccgccggccc tgggctcgag aggacggtgc atctcgaggg catgctcccc
    15661 agcaccgcga cgctgcttgt tcagaagggg tacgcggtgc tctgcgacga ggggatggag
    15721 gtaccgttca gcgccccctt cgctcagctt cttatggaga ccgcctcggg ggcgttggag
    15781 gagaccgagg ccttgacctt cggcccctcc gattcattcc tggtcatcaa cgaggaccgg
    15841 ggaacgacca tcgtcgcgct cagcccatga acctttccgc gatgtacccc acggcctcga
    15901 cgaactggcg catctcctcc tcggtgttgt aaaggtacaa cgaggcgcgg gcgcacccct
    15961 ccatgcctcg ggcggcgaag tagggatgga cgcagtgcat gcccgagcgg atgaggacgg
    16021 aggcgatctc gtccaggatc atcgccacgt catgcgggag catgcccctc aggttgaagg
    16081 agaggatccc gcccctccgg cggggatcgg agggacctag gatgctcagt ccttcgatgg
    16141 ccgacaggcc ctcggtggca agggcgttca gccgatgctc gtgctccacg atctcctcca
    16201 tcccgacctc cctgaggtag cggaccgccg ctccgctgcc gatgatgccg gcgaagttct
    16261 gaagcccggc ctcgaacctc tccgggaccg gaagcagctc cgctgaatcg taggaggtga
    16321 tcgccaccgc ccctccgccg gtgaccaggg gctctatccc ctccaggacc tccctctttc
    16381 catacaatgc ccccatcccc gaaggcccga gcatcttgtg catggaccag gcgtacaggt
    16441 cgacgtccgt cctgtccagg gccagggggc tgtgcggggc ggtctggcag ccgtcggcca
    16501 tgaccagcgc cccccggtca tgcgccagct ccaccacggc ctccaggggg atggtgctgc
    16561 cgtccacgtt gttggtgtgg accacgctca ccagccgggc gcccttctcc agctcctcct
    16621 tcaggggctc caggtccccc tgctccagcg ccgagaaggg gacgatgccc cggcggatgc
    16681 ccctctccct cgccagaagc acccagggca catggttgct gttgtgctcc atgtccgtgg
    16741 tgatcaccct ctcccccttc ctgaggcgca gccccttggc cactgtgttc agggactccg
    16801 tggcgctctt ggtgaagacg acgcactcgg ggtccctcgc cccgacgaag gcggcgatct
    16861 cctccctcgc cctgtccatg cggaccgaca cctcggtggc caggcggtgg acgctccggc
    16921 cgccgcaggc cgggaaggcg cagtagtact cgtccatggc ctcgatgacc tggcgggggc
    16981 gcaggctctg acaggcgctg tccagataga tcggtgggct ggcctgctgg tacaacggga
    17041 agtccttccg gacccggttc acgtccattc cagtcggggc tgggaacgga ttggatccta
    17101 ttaacgcctt tgtgtaaaaa aaggaggt
//
LOCUS       DAOSYN010000008        16796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2843_length_16796_cov_4.062601, whole genome shotgun sequence.
ACCESSION   DAOSYN010000008 DAOSYN010000000
VERSION     DAOSYN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 16796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16796
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2843_length_16796_cov_4.062601"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PLJ11_00775"
     CDS             complement(<1..161)
                     /locus_tag="PLJ11_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOO03239.1"
                     /translation="MIDLGRALKTAMTTGKVVFGVEQTEKTVRNGEAKLVIVAKNCPS
                     GFLVSKDHGV"
     gene            complement(158..1711)
                     /gene="rpoA2"
                     /locus_tag="PLJ11_00780"
     CDS             complement(158..1711)
                     /gene="rpoA2"
                     /locus_tag="PLJ11_00780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HOO03240.1"
                     /translation="MAQKDFIKGLERKGVAVETIGIVKDHFTNQTDLAKASVKELQGI
                     GLSKEAAEELLAKLGKRERAGKAPAKAKAAPKRRADRKEAEPETAVVFEIPDKSKRHT
                     PFEEQLLRRCEALKLQLPQRIILNLARRLEGNDISTEDLEKILQVANQRYQDHMMDPN
                     ESAGILAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIVDARRVPSTPMMTLY
                     VKKELSNDVETVKAIASNIEMTTVLDLADIETDLHNMRLFIVPDLRKCMQKGLVDQDG
                     RPPRGEPSPIELIREKVSRLRGIVVVDEVAREALPPKVELAGEIGTGYLCLEPKEPSF
                     KNLQKALDLVKGAKVKGVEGITRALLKKKDDQWIIYTEGSNLREVLKIDGLDNTRAYS
                     NSIQEVYEVLGVEAARNSIIEEASRTLEEQGLSVDIRHIMLVADLMTNDGDVKAIGRH
                     GISGRKSSVLARAAFEITAAHLLTAAMTGEEDHLDGVAENIIVGQPVTLGTGAVNLVY
                     APVKKGAAE"
     gene            complement(1711..>3931)
                     /locus_tag="PLJ11_00785"
     CDS             complement(1711..>3931)
                     /locus_tag="PLJ11_00785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HOO03241.1"
                     /translation="PDADLIPLGIDPAACRPEWMVLTALPVPPVTVRPSITLESGDRS
                     EDDLTHKLVDVLRINQRLRENRDAGAPQLIVEDLWELLQYHVTTYFDNQTSGIPPARH
                     RSGRPLKTLVQRLKGKEGRFRSNLSGKRVNFSARTVISPDPWLSINQVGVPVFAAREL
                     TVPVTVTKDNIEVLKAMVKRGPTPAGTVYEPGVNYVIRQDGRRIRVTDRNAETVAEGI
                     EIGYVVERHLMDGDVVLFNRQPSLHRMSMMAHVVKVMPGKTFRLNLCVCPPYNADFDG
                     DEMNLHVLQSEEARAEAHILMKVQEHILSPRFGGPIIGAIHDHITGMFQLTYMNPRFD
                     KAQTLSILSKMGNNRLPEPTVEGGREYWAGKDLFSTILPKDLNIQFKAHICQKCPKCE
                     KEGCQYDAYVKIRDGRLLMGTIDDNAIGSGDGKILDKIARDYGADRAREFLDDVTRLA
                     IGSIMVRGFSTGIDDEDIPEEAKRQIDDKINNTFREVDKLVASYRDGTLDQMPGRSLE
                     ETLEVEVMKKLGEARDEAGRIAGKHLGLNNSAVQMAKSGARGSMLNLSQMAGCIGQQA
                     VRGERLSRGYWNRTLPHFQKGDLGAQAKGFVKNSYKSGLTPTEFFFHSMGGREGLVDT
                     AVRTSRSGYMQRRLINALEDLKLKQDGTVRNTADSIIQFKYGEDGIDPTRSVQGDAID
                     VDDVLRDVLGEHYADLLARAEERKISAYATVEKDLMEAAMEEEEESEEIEYEGGGEE"
     assembly_gap    3932..3941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3942..4478)
                     /locus_tag="PLJ11_00790"
     CDS             complement(<3942..4478)
                     /locus_tag="PLJ11_00790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505506.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HOO03242.1"
                     /translation="MMRGVSKRIGSIKFACLSPDEIRKMSATKIITADTYNDDGFPIE
                     AGLMDTHLGVIEPGLMCKTCGHKVDECPGHFGHIDLAMPVIHVGYVKEIKKLLQATCK
                     SCGRLVLTEEDVKEYRARMDTMEELGSDTMDLREFSKETAKAAAEKAMKEVCPHCGEE
                     QKKITLDKPTTFREDGHKL"
     gene            complement(4475..8074)
                     /locus_tag="PLJ11_00795"
     CDS             complement(4475..8074)
                     /locus_tag="PLJ11_00795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HOO03243.1"
                     /translation="MRDLVDLYFKENSIVNHHIASFNDFLSSQSNPNSRMQKIVDNVR
                     VSAEDPERGMITLDPEKTNGRIIQIRVGRRRDEKTGQVDQHLPPTLKVGEPMVREANG
                     YVHNITPMEARLRNLNYVAPMHLDFTIIEDGIEREEKDVLIGDLPIMVKSRKCNIFKE
                     NMEKEYDLTDEQYNAELMKRSEDPLDAGGYFIIGGTERVLITLEDLAPNRVMVELSER
                     YGTAMEVAKVFSQKEGHRALTLVEKKRDGMLMVTVPAASGQIPLVALMKALGMENDED
                     IYEAIVSAPEMANIVYANLEEIQDHKQYGPTGIFTTEQALTYLEKKFATGQAKEYRVK
                     KVESIIDRSLLPHLGDTKDDRMKKAIFLGRIARSVLELSLGKRKEDDKDHYANKRLKL
                     SGDLMEDLFRVAFTNLIKDLKYQLERSYARKKDLRIASTIRPDLMTHRLLHALATGNW
                     VGGRAGVSQLLDRTSNMSTVSHLRRITSSLTRSQPHFEARDLHPTQWGRLCPNETPEG
                     QNCGLVKNAALIINVSEGFREEDVTFLLKDLGVREVRGQQMTGTRVYVNGDLKGVHDN
                     ARDLIAQMRNRRRTGLLSDEINIRLDEEMDEVIINCDEGRLRRALLVLKDGHLVITRR
                     HLEDIRAGKLRWSDLVREGVLEWIDAEEEEDTYIAVEPFEVPMKCGNCDRSISPMDMD
                     WMNPGTADRQAELKCRHCGGITKVDYLFTKEHTHMEVDPMVILGVCSGMVPYPEHNSS
                     PRVTMGAGMAKQSLGLAASNYRKRPDTRGHILHYPQKPMVQTKFMDFVAFNQRPAGQN
                     FVVAVLSYHGYNMEDALILNQSSVERGLGRSTFMRTYRTEERRYPGGQEDHFEIPSPD
                     VRGARTDAAYNNLSEEDGLISPETAVTGGDVLVGKTSPPRFVEEEEANFLSGHKRRET
                     SITVRAGETGWVDSVMLTESENGSRLVKVKVRDERIPELGDKFASRHGQKGVVGLLVP
                     AADMPFTADGVVPDLVINPHAIPSRMTVAHVLEMIGGKVGALEGRHVDGTAFSGEREE
                     ALRQALLDAGFQRSGREVMYDGLTGRMIQADVFIGVIYYQKLHHMVSGKMHVRSRGPV
                     QILTRQPTEGRSRQGGLRFGEMERDCLIGHGAAMVIKDRLLDESDGTFQWVCGNPNCG
                     HIAILDKRGALRCPVCGNNTNVHMIQTSYAFKLLLDELLSLGVAMRLQLEDLR"
     gene            complement(8080..8346)
                     /locus_tag="PLJ11_00800"
     CDS             complement(8080..8346)
                     /locus_tag="PLJ11_00800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HOO03244.1"
                     /translation="MEDAVPFNVLLHKMVPVHELLSDEETEKVLKRLRITRDQLPKIK
                     DSDAAIKTLEKAMNRPIPEKSVIRIVRDSYTSEVAEVYRLVIRG"
     gene            complement(8469..8602)
                     /locus_tag="PLJ11_00805"
     tRNA            complement(join(8469..8551,8582..8602))
                     /locus_tag="PLJ11_00805"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8536..8538),aa:Lys,seq:ttt)
     gene            9107..9274
                     /locus_tag="PLJ11_00810"
     CDS             9107..9274
                     /locus_tag="PLJ11_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOO03245.1"
                     /translation="MANEKICSSCGVRLVGKGTTFFKCPICGVEEIGRCAQCRDQSVK
                     YECKKCGFVGP"
     gene            9285..9551
                     /locus_tag="PLJ11_00815"
     CDS             9285..9551
                     /locus_tag="PLJ11_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HOO03246.1"
                     /translation="MGTVAATYSLMPEDIDFDFKALIAELPKVVPASIRVAKTEVVPM
                     AFGLKRLEAVFVMEDAAGLVDRLEEALRGLAGIQNVETEQVTLL"
     gene            9602..>10528
                     /locus_tag="PLJ11_00820"
     CDS             9602..>10528
                     /locus_tag="PLJ11_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03247.1"
                     /translation="MPEVVIFDGYMDEPGSLGVPPYIHPLPRAAFGAVRAAGGVPHYI
                     TVDEWRAGRAVPPCDMLIVLAGMSVPGRYLRGMPASRREVLQLIEGQRGETVLGGPAA
                     LDPELRGRFRHAHYLDAAAAAYDLLDNGRARDRWRTMEEWDLWSMLGADCVLHHPDHP
                     QPLIAELETYRGCVRYLTGGCSFCVEPLKGRPVFREPEAVIAEARALRERGVVNFRLG
                     AQTCIISYKAELDGTDCPRPSPEALEELLSGMAALSPQVLHVDNANPAVMARHPEETE
                     RALGALVAHCTPGNVLALGMETADPAVVATNNL"
     assembly_gap    10529..10538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10539..11205
                     /locus_tag="PLJ11_00825"
     CDS             <10539..11205
                     /locus_tag="PLJ11_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03248.1"
                     /translation="VRAINRAGGHRGANGLPHLLPGINVLVGLEGERPETLEMDLRFL
                     KGILDEGLLLRRINIRQVVPVRRAFERTVSHSQFLRFKERVRTEVDRPMLERLVPVGT
                     VLRSVYTELREGKVTFGRQIGTYPLLVGIPHPVELGRFYDVAVVGHGFRSISAVEFPL
                     AVNSCSIAALEALPGLGRKRAVRLFRARPLEGPDAVRRALDDPRVADAVLPFLSFGRP
                     NPN"
     gene            11231..12274
                     /locus_tag="PLJ11_00830"
     CDS             11231..12274
                     /locus_tag="PLJ11_00830"
                     /inference="COORDINATES: protein motif:HMM:NF016030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase large subunit PriL"
                     /protein_id="HOO03249.1"
                     /translation="MEYRDLARYPFLKEATQFVKERQLGLEDLVSSTFAAEARRRGKA
                     RVLTALSDLTVPNGPVTSEEEAAQEVLSYPYARLLVSCIGDEALVRRYALAEAKAFNE
                     RLRRDDMGTVIMLAGELGVEAALAGDSLRMHFTDFLGYTSGLRGPEWKLVNQDLRRGY
                     VLLEKARFVRVLEQALDERIEDELPLPVNDELLEAVRGDLAEVVAALEAYKERYRDEG
                     FGEVSILKFPPCMRKLVAMAQAGQNMPHAGRFALTSFLSFIGMPVDDIIGLFSGSPDF
                     DRSKTEYQVRHITGDGAGKRYTPPECATMRTNGLCFEPDELCNGRRVRHPLTYYRIRS
                     HEKAGEGKGARPS"
     gene            complement(12261..12896)
                     /locus_tag="PLJ11_00835"
     CDS             complement(12261..12896)
                     /locus_tag="PLJ11_00835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOO03250.1"
                     /translation="MVLDSHRSKVDFIMDPVAKAMVKVPPNHISLIALALAALAGALL
                     YLSHAHWELLPVAAVVVLVSGFFDGLDGKVARLVGKADRTGDFLDHVLDRYADMLMIG
                     GVAVSAWCSPYLGMMALVGVLLTSYMGTQAQAVGAKRMYAGLLGRADRIVLSFLAPLV
                     QLGMVLLGSPEVALLGYEINAFEAMMLWLAVVGNLTAIQRAVLTYSWLRKA"
     gene            complement(12887..13411)
                     /locus_tag="PLJ11_00840"
     CDS             complement(12887..13411)
                     /locus_tag="PLJ11_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HOO03251.1"
                     /translation="MLVALTGTPGTGKSTVAAAVRTKGWNVLEVNELARRHGLLRGKD
                     PARDSYEVDPEELQEALGREGYAEGILVGHLAHLLDVDMVIVLRCRPEALAVRLERRG
                     WPAAKIRENVLAEALDIILAEAMGSGVPVFEVDTTGLGQEETAEAVLAILAGEREKYA
                     AGNIDWSEEALRWS"
     gene            complement(13560..14378)
                     /gene="truA"
                     /locus_tag="PLJ11_00845"
     CDS             complement(13560..14378)
                     /gene="truA"
                     /locus_tag="PLJ11_00845"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOO03252.1"
                     /translation="MVRLAVKFAYDGTCFMGSQRQPGERTVEGELLSALVAIGAIGSA
                     AKGRFRVASRTDRGVSALGNVFAVDTSFRPKELLAALNAKCRDVHCYALAEVPAAFSP
                     RRAKDRWYRYHLPYRGQDIALMRAAAKEFEGRHEFRLFCRPEGRATLRSLGAVEVRPM
                     AGCIVIDLRAREFLRNMVRRIVSALDQVGQGKVSLGELREALEGRGRAMGLADPASLF
                     LMDVDLGLEWTYPPTGRMPTAAAEGAHRARVRLLFEEALVEMSKLAEKAPNNSI"
     gene            14462..15163
                     /locus_tag="PLJ11_00850"
     CDS             14462..15163
                     /locus_tag="PLJ11_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOO03253.1"
                     /translation="MPQFKVVLVGPRVDGNVGAVARSMANFGFAELCMVRPCELTEEA
                     FRRAKHAGDILRGAQVVQSFEEAVADCFHVVGTSGKVTPGEKHYIRIPLSPREFAERV
                     EGVEEKVALVFGPEDTGLTQEELARCDVLVSIPAHEDYPVLNLSHAVAIVLYELYQRH
                     IHIGSPRKAGESEREMLQRFFDELLDAIDYPEFRRERTSVMFRRMMGRAMPSKWEFHT
                     IMGVFGDATKIIRGR"
     gene            complement(15164..16195)
                     /gene="ftsZ"
                     /locus_tag="PLJ11_00855"
     CDS             complement(15164..16195)
                     /gene="ftsZ"
                     /locus_tag="PLJ11_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO03254.1"
                     /translation="MGARFEGLEEPEVELHELSILVVGCGGGGCNSIHRLNLLGIERA
                     TTIAINTDQKSLKATECPNRLLLGVEYTKGLGTGGRPDIGEECALNAAPMLSKLFHDV
                     DLTFIVTGLGGGTGTGASPVVADIARRSGSMVISIATTPFSFEGANRRNIAMKGLKRL
                     REASNSLLVLDNDRLLQMVENLPVDQGLAIIDQLISELIKGTVDILTVPSLINLDFAD
                     LRTIMEHGGVSTILYGENADPDGVVRDAINSPLLDVDIRGGTGALIHISGGSNLTMRR
                     ANKIVSSITKVLDEDATVKFGVRVDEGMEGNIRMIAIVTGISEVSERFPPSVAPGDDL
                     GKVLGKYGP"
     gene            complement(16223..16534)
                     /locus_tag="PLJ11_00860"
     CDS             complement(16223..16534)
                     /locus_tag="PLJ11_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOO03255.1"
                     /translation="MSDSERITVRIQADKLGALQALVDGGKFPTISDAIRAAIDSFIE
                     TNFTPEHIQRVTVELPKVKVVELESLVKEGDSVSIDDAIRNAVREYVRMRLDKAMGEL
                     R"
BASE COUNT         2644 a         5473 c         5336 g         3323 t
ORIGIN      
        1 acgccgtggt ccttggagac caggaacccg ctggggcagt tcttggccac aatcacgagc
       61 ttggcctccc cgttcctaac tgtcttttcc gtctgctcaa cgccgaagac caccttcccg
      121 gtggtcatgg cggtctttaa ggctcttccc agatcgatca ttcagctgcc cccttcttca
      181 caggtgcgta cacgagattc acggccccgg tcccgagtgt aaccggctgc cccactataa
      241 tgttttctgc cacgccgtcc aggtggtcct cctccccggt catggccgcg gtcagcaggt
      301 gcgcggcggt gatctcgaac gccgccctgg ccagcactga cgacttcctc ccggatatcc
      361 catggcggcc gatggccttg acgtcgccgt cgttggtcat caggtcggcc accagcatga
      421 tgtggcgtat gtccacggac agcccctgct cctccagggt gcgggacgcc tcctcgatta
      481 tggagttcct ggcggcctcc acccccagca cctcgtacac ctcctggatg gagttgctgt
      541 atgcccgggt gttgtccagc ccatcgatct tgagcacctc ccgcaggttg ctgccctccg
      601 tatagatgat ccactggtcg tccttcttct tgagcagggc gcgggtgatg ccctcgaccc
      661 ccttgacctt ggcccccttg accaggtcca gggccttctg caggttcttg aaggacggct
      721 ccttgggctc gaggcacagg tagccggtcc cgatctcccc ggccagctcg accttgggag
      781 gcagggcttc ccgggccacc tcgtccacca ccacgatgcc cctgagccgg gagaccttct
      841 cccggatgag ctcgatcggg gaaggctcgc ccctgggcgg ccgcccgtcc tggtccacca
      901 gccccttctg catgcacttc cgcaggtcgg gcactatgaa cagacgcata ttgtgcaggt
      961 ccgtctcgat gtccgcgagg tccagcaccg tggtcatctc gatgttggag gcgatggcct
     1021 tcacggtctc cacgtcattg gacagctcct ttttcacata cagggtcatc atcggggtgc
     1081 tggggacgcg gcgggcgtcc acgatctcga tgaggcgggg caggcccagg gtcacgttga
     1141 tctcggccac cccggcgtag tggaaggtac gcatggtcat ctgcgtgccc ggctccccga
     1201 tgctctgggc cgcgaggatg ccggcgctct cgttggggtc catcatgtgg tcctggtacc
     1261 tctggttggc cacctggagg atcttttcaa ggtcctccgt gctgatgtcg ttgccctcga
     1321 ggcggcgggc gaggttcagt atgatcctct ggggcagctg cagcttgagc gcctcgcacc
     1381 tcctgagcag ctgctcctcg aacggggtat gcctcttgct cttgtcgggg atctcgaaga
     1441 cgaccgccgt ctcgggctcg gcctccttcc tgtccgccct cctcttcggg gcggccttgg
     1501 ccttggccgg ggccttgccg gccctctccc tcttccccag cttcgcgagc agctcctcgg
     1561 cggcctcctt gctcaggccg atgccctgca gctccttgac ggacgccttc gccaggtcgg
     1621 tctggttggt gaagtggtcc ttcacgatcc cgatggtctc aacggcgacg cccttcctct
     1681 ccagcccctt gatgaaatcc ttctgggcca tcactcctca cctccgccct cgtactcgat
     1741 ctcctccgac tcctcctctt cctccatggc ggcctccatg aggtccttct ccacggtggc
     1801 gtaggcgctg atcttgcgct cctccgcacg ggccagaagg tcggcgtagt gctccccgag
     1861 cacgtcgcgc agcacgtcgt ccacgtctat cgcgtccccc tgcacgctgc gggtggggtc
     1921 gatgccgtcc tccccgtact tgaactggat gatgctgtcc gcggtgttgc ggaccgtccc
     1981 gtcctgcttc agcttcaggt cctccagggc gttgatcagc cggcgctgca tgtaccccga
     2041 ccgggaggtg cgcacggcgg tgtcgaccag cccctcgcgc ccgcccatcg agtggaagaa
     2101 gaactccgtg ggcgtgaggc cgctcttgta ggaattcttg acgaacccct tggcctgggc
     2161 gcccaggtcc cccttctgga agtgagggag cgtccggttc caatagcccc tggagaggcg
     2221 ctcgccccgg accgcctgct gccctatgca ccccgccatc tgcgagaggt tgagcatgga
     2281 gccgcgggcc cctgacttgg ccatctgcac cgcggagttg ttcagcccca ggtgcttccc
     2341 ggcgatcctt cccgcctcgt ccctggcctc cccgagcttc ttcatgacct ccacctccag
     2401 ggtctcctcg agcgagcgtc cgggcatctg gtccagggtc ccgtcgcgat aggaggcgac
     2461 cagcttgtcg acctcgcgga aggtgttgtt gatcttgtca tctatctgcc tcttcgcctc
     2521 ctccgggatg tcctcgtcgt cgatgccggt ggagaagccg cggaccatta tggacccgat
     2581 ggccaatctc gtcacgtcat ccaggaactc cctggcccgg tcggccccgt agtccctcgc
     2641 tatcttgtcc aggatcttgc cgtcaccgga gccaatggcg ttgtcatcga tggtgcccat
     2701 cagcagcctg ccgtcgcgta tcttgacata tgcgtcgtac tggcacccct ccttctcgca
     2761 cttggggcac ttctggcaga tgtgcgcctt gaactggatg ttgagatcct tcgggaggat
     2821 ggtggagaac aggtcctttc cggcccaata ctcccttccc ccctccaccg tcggctcagg
     2881 cagcctgttg ttccccatct tggagaggat ggacagggtc tgcgccttgt cgaagcgggg
     2941 gttcatgtat gtcaattgga acatgccggt gatgtggtcg tggatggccc cgatgatcgg
     3001 ccccccgaac ctcggggaca ggatgtgctc ctgcaccttc atcaggatgt gcgcctccgc
     3061 cctcgcctcc tcgctctgca ggacgtgcag gttcatctcg tccccgtcga agtcggcgtt
     3121 gtacggcggg cagacgcaaa ggttcaggcg gaaggtcttc ccgggcatga ccttcacgac
     3181 atgggccatc atggacatgc ggtgcaggga gggctgcctg ttgaacagga ccacgtcccc
     3241 gtccatcagg tgcctctcga ccacgtagcc gatctcgatg ccctcggcca cggtctccgc
     3301 gttgcggtcc gtaacgcgga tcctgcggcc gtcctggcgg atgacgtagt tcacgcccgg
     3361 ctcgtagacc gtcccggcgg gggtggggcc acgcttgacc atggccttca gcacctcgat
     3421 gttgtccttg gtgacggtga cggggacggt gagctcgcgc gcggcgaaca ccggcacgcc
     3481 gacctggttg atggacagcc atgggtcggg ggagatcacc gtgcgggcgg agaagttcac
     3541 cctcttgccg gacaggttgg agcggaagcg cccctccttc cccttcagcc tctgcaccag
     3601 tgtcttcaac ggccggccac tgcggtgcct ggccggcggg atgccggacg tctggttgtc
     3661 gaagtaggtg gtgacatggt actgcagcag ctcccacagg tcctccacga tgagctgggg
     3721 ggcccctgcg tccctgttct cgcgcaacct ctggttgatg cgcagcacgt cgaccagctt
     3781 gtgggtcagg tcgtcctcgg accggtcccc cgactcgagc gtgatggacg gcctgacggt
     3841 gaccggcggg accggcaggg cggtgaggac catccattcc ggccggcagg ccgcggggtc
     3901 tattcccagc gggatcaggt ccgcgtcggg gnnnnnnnnn ngagcttgtg cccatcctcc
     3961 ctgaaggtcg tgggcttgtc cagcgtgatc ttcttctgct cctcgccgca gtgcgggcag
     4021 acctccttca tagccttctc ggcggcggcc ttggcggtct ccttggagaa ctcgcgtaga
     4081 tccatggtat cgctccccag ctcctccatg gtgtccatgc gggcgcggta ctccttgacg
     4141 tcctcctccg tgaggaccag ccttccgcag gatttgcagg tcgcctgcag cagcttcttg
     4201 atctccttca catagccaac gtggatgacc ggcatcgcca ggtcgatgtg cccgaagtgc
     4261 ccggggcact cgtccacctt gtgcccgcag gtcttgcaca tcagcccggg ctcgatgacg
     4321 cccaggtggg tgtccatcag cccggcctcg atcgggaacc cgtcatcgtt gtaggtgtcg
     4381 gcggtgatga tcttggtggc cgacatcttg cgtatctcgt ccggggacag gcaggcgaac
     4441 ttgatggagc cgatcctctt ggaaacccct cgcatcatct caaatcctcc agttgtaaac
     4501 gcatggcgac cccgagcgag agcagctcgt caagcagcag cttgaaggcg taggacgtct
     4561 ggatcatgtg gacgttggtg ttgttgccgc acactggaca gcgcagggcg cccctcttgt
     4621 ccaggatggc gatgtgcccg cagttggggt tgccgcacac ccattggaag gtgccgtccg
     4681 attcgtccag caaccggtcc ttgatgacca tcgccgcccc gtgcccgatc aggcagtccc
     4741 tctccatctc cccgaacctg agcccgccct gcctggagcg gccctccgtc ggctgtcggg
     4801 tgaggatctg cacggggccg cgggaccgca cgtgcatctt gccggagacc atgtggtgca
     4861 gcttctgata gtatatgacg ccgatgaaga cgtcggcctg gatcatgcgt ccggtcaggc
     4921 cgtcgtacat cacctcgcgc ccggacctct ggaagccggc atcgagcagc gcctggcgca
     4981 gggcctcctc gcgctcgccg ctgaaggccg tcccgtccac gtgccgccct tcgagcgcgc
     5041 cgaccttccc gccgatcatc tccagaacgt gcgcgacggt catacgggac gggatggcgt
     5101 gggggttgat gaccaggtcc gggacgaccc catcggccgt gaagggcatg tcggccgccg
     5161 ggaccaatag cccgacgacc cccttctgcc cgtgcctgga ggcgaacttg tcccccagct
     5221 cggggatcct ttcgtccctg accttgacct tcaccaggcg ggagccgttc tccgactcgg
     5281 tgagcatgac gctgtccacc cagcctgtct ccccggcccg gacggttatg gacgtctccc
     5341 gtctcttgtg cccggacagg aagttcgcct cctcctcctc gacgaacctc ggcggggagg
     5401 tctttccgac cagcacgtcc ccgccggtga ccgccgtctc cggggagatc agcccgtcct
     5461 cctcggacag gttgttgtac gcggcgtcgg tcctggcccc gcgaacgtcg ggagagggaa
     5521 tctcgaagtg gtcctcctgg ccgccggggt acctgcgctc ctccgtgcgg tacgtccgca
     5581 tgaacgtgga gcgccccagg ccccgctcca cgctgctctg gttgaggatg agcgcgtcct
     5641 ccatgttgta gccgtggtag gagaggaccg cgacgacgaa gttctgcccc gctgggcgct
     5701 ggttgaaggc cacgaagtcc atgaacttgg tctggaccat cggcttctgt ggatagtgca
     5761 atatgtgccc gcgggtgtcc gggcgcttgc ggtagttgct cgccgccagc cccagggact
     5821 gcttggccat ccccgcgccc atggtgacac ggggcgagga gttatgctcc ggatagggga
     5881 ccatgcccga gcataccccg aggatgacca tcggatcgac ctccatgtgc gtgtgctcct
     5941 tggtgaacag gtagtcgacc ttggtgatgc cgccgcagtg ccggcacttg agctcggcct
     6001 gcctgtcagc ggtccccggg ttcatccagt ccatgtccat gggggagatg ctccggtcgc
     6061 agttcccgca tttcatcggg acctcgaacg gctccacggc gatgtaggtg tcctcctcct
     6121 cctcggcgtc gatccattcc agcacgccct cgcgcaccag gtcggaccag cgcagcttcc
     6181 cggcacggat gtcctccagg tgcctgcggg tgatcaccag gtggccgtcc ttcaggacca
     6241 ggagcgcccg gcggaggcgg ccttcgtcgc agttgatgat gacctcgtcc atctcctcgt
     6301 ccaagcggat gttgatctcg tcggacagga ggccggtgcg gcggcgattc cgcatctggg
     6361 cgatgaggtc gcgggcgttg tcatgcaccc ccttgaggtc cccgttcacg tagacgcggg
     6421 tgccggtcat ctgctgcccc ctgacctcgc gcacccccag gtccttgagc aggaaggtga
     6481 cgtcctcctc acggaagccc tccgagacgt tgatgatcag ggcggcgttc ttgaccagcc
     6541 cgcagttctg cccctcgggg gtctcgttcg ggcacagccg gccccattgg gtcgggtgca
     6601 ggtcccgggc ctcgaagtgg ggctggctgc gggtgaggga cgatgtgatc ctcctcaggt
     6661 gggagacggt gctcatgttg gacgtgcggt ccagcagctg gctgaccccc gcccttccgc
     6721 ccacccagtt cccggtcgcc aacgcgtgga ggaggcggtg ggtcatgagg tcggggcgga
     6781 tggtgctggc gatgcgcagg tccttcttcc tggcatagga gcgctccagc tgatacttga
     6841 ggtccttgat cagattggtg aaggcgaccc ggaacaggtc ctccatgagg tcgccggaga
     6901 gcttcaggcg cttgttggcg tagtggtcct tgtcgtcctc cttgcgcttg ccgagggaga
     6961 gctcgagcac ggagcgggcg atcctgccca ggaatatggc cttcttcatg cggtcgtcct
     7021 tggtgtcccc tagatgggga agaagggagc ggtcgatgat ggactcgacc ttcttgaccc
     7081 tgtactcctt ggcctggcca gtggcgaact tcttttccag gtaggtgagg gcctgctcgg
     7141 tggtgaagat gcccgtcggc ccgtactgct tgtggtcctg gatctcctcc aggttggcgt
     7201 agacgatgtt cgccatctcc ggggcggaga cgatggcttc gtagatgtcc tcgtcgttct
     7261 ccatccccag ggccttcatc agggcgacga gcgggatctg ccccgacgcc gctgggacgg
     7321 tgaccattag catgccgtcg cgcttcttct cgacgagggt aagggcgcgg tggccctcct
     7381 tctgcgagaa gaccttggcc acctccatgg ccgtgccgta ccgctcgctg agctccacca
     7441 tgacgcggtt aggcgcgagg tcctccaggg tgatgaggac ccgctccgtc cctccgatga
     7501 tgaagtaccc gcccgcgtcc agggggtcct cgctcctctt catgagctcg gcgttgtact
     7561 gctcgtcggt caggtcgtac tccttctcca tgttctcctt gaagatgttg cacttgcggg
     7621 acttgaccat gatcggcagg tccccgatca ggacgtcctt ctcctcccgc tcgatgccgt
     7681 cctcgatgat ggtgaagtcc aggtgcatgg gggccacata gttcaggttc ctgagcctgg
     7741 cctccatggg ggtgatgttg tgaacgtagc cgttcgcctc gcggaccatg ggctccccga
     7801 ccttcaaggt cggcgggagg tgctggtcca cctgccctgt cttctcgtcc cggcggcgcc
     7861 cgacgcggat ctggatgatc cgcccgttcg tcttctccgg gtccagtgtt atcatcccgc
     7921 gctcggggtc ctccgcagaa acccgcacgt tgtcaacgat cttctgcatc ctgctgttcg
     7981 gattgctctg ggacgagagg aagtcgttga agctggcgat gtgatggttg acgatgctgt
     8041 tctccttgaa gtagagatct acgaggtccc tcactaggtt caccctctga ttaccaggcg
     8101 gtagacttcc gccacctcgg acgtatagct gtccctgacg atcctgatga cgctcttctc
     8161 cgggatgggc ctgttcatgg ccttctccag cgtctttatc gccgcgtcgc tgtccttgat
     8221 cttgggaagc tggtcgcggg ttatcctgag ccgcttgagg accttctcgg tctcctcgtc
     8281 gctcaatagt tcgtgcactg gcaccatctt gtgcaacaat acgttaaatg gtacggcatc
     8341 ctccaagcga atcacctcat accatgcaga aaccgggctg ttcctccact aagaattggc
     8401 cttataaaaa cgcatcggga atcgggtttg cttctttcgg tctgcatttc tcttttcacc
     8461 tacccaagcg ggcccgcggg gatttccgca tacacggaac ttccgcgtac cattcgaacc
     8521 ccggaccacc tggttaaaag ccagatgctc tctctaggga cccttcgtct ccgaaggtcc
     8581 tacctagctg agctacgggc ccattggtaa gtaagtgccg gcgtgtgaac gggagagtaa
     8641 acccttgtat tatttaacgt ttcccatcca ccacggaaca ttagtcctgt tggcacaccg
     8701 agcaacatgg acccgccgga tgttcctgcc attccttcgg tattgagggt aatgtatatg
     8761 aacttgcgct taggaaaagg ctagaggcct tggcggcacc tggatgcggg agcggcattg
     8821 gaagggccgt ggatggtccc ctctatttcc tctattatcc tctattattt cctctattat
     8881 tatctgagat aatatctttg atcatatctt cgtatctttg atcatatctt cgatcacatc
     8941 ttcgatcttc catatcttcg atcttcccct cgggacccct cagggcttcg ggccccatca
     9001 cccttatgga tggccgtgag aatggcccca gtgcgcccat tggccatgcg gaccgcccaa
     9061 tcacgttttt ataggaggac tataatctcg cacaaagcga tgacctatgg cgaatgaaaa
     9121 gatctgcagc tcatgcggtg tccggctggt gggcaaaggc accaccttct tcaaatgccc
     9181 gatctgtggc gtcgaggaga tcggccgctg cgcccagtgc cgggaccaga gcgtcaagta
     9241 cgaatgcaag aagtgcggat tcgtcggccc gtaaggtggg agcgatgggg accgttgcag
     9301 cgacctattc cctcatgccc gaggacattg atttcgactt taaggccctg atcgccgagc
     9361 tgcccaaggt ggtcccggcc tccataaggg tggccaagac ggaggtcgtg cccatggcgt
     9421 tcgggctcaa gcggctggag gcggtcttcg tcatggagga cgccgccggg ctggtggaca
     9481 ggctggagga ggccctgcgc gggctggccg gcatccagaa cgtcgagacc gagcaggtca
     9541 ccctgctctg aggtctcaaa ggcctgccag ggcccccttt ccttttctca ttggagctac
     9601 cgtgcctgaa gtggttatct tcgatggcta catggacgag ccaggctcct tgggcgtccc
     9661 gccgtacatc cacccgcttc caagggcggc cttcggggcg gtgcgcgccg ctggaggcgt
     9721 ccctcattat atcacagtgg atgagtggcg agcggggagg gcggtgccgc cctgcgatat
     9781 gctcatagtc ctggcgggca tgtccgttcc aggcaggtac ctccggggca tgcccgcctc
     9841 ccgccgggag gtcctgcagc tcatcgaggg gcagagaggg gagacggtgc tgggggggcc
     9901 cgcggccctg gacccggaac tccgggggcg cttccgccac gcgcactacc tggacgccgc
     9961 cgcggccgcc tacgacctgc tggacaacgg gcgggcaagg gatcgctggc gcaccatgga
    10021 ggaatgggac ctgtggtcca tgctgggggc ggattgcgtc ctgcaccatc cggaccaccc
    10081 ccagcccctg atcgctgagc tggagaccta cagggggtgc gtgcgctacc tgaccggggg
    10141 atgctccttc tgcgtggagc cgctgaaggg ccggccggtc ttccgggagc cggaggccgt
    10201 gatcgccgaa gcccgggcct tgagggagag gggggtggtc aacttccgcc tgggcgcgca
    10261 gacctgcatc atctcctaca aggcggagct ggacggcacc gactgccccc ggccctcccc
    10321 ggaggccctg gaggaactcc tctccggcat ggcagccctc tcgccgcagg tgctgcatgt
    10381 ggacaatgcc aacccggcgg tgatggccag gcatccagag gagacagaac gggccttggg
    10441 cgcgctggtg gcccattgca cccccgggaa cgtgctcgcc ctgggcatgg agacggcgga
    10501 ccccgcggtc gtggccacga acaatctgnn nnnnnnnncg tccgggccat caacagggcc
    10561 ggtgggcaca ggggcgccaa cggccttccc catctcctgc cgggcataaa cgtcctcgtg
    10621 ggcctcgagg gggagcgacc agagaccctg gagatggacc tccgcttcct caagggcatt
    10681 ctggacgagg gactgctcct gcgccggatc aatatccggc aggtcgttcc ggtccgaagg
    10741 gcgttcgaac ggaccgtgag ccactcccag ttcctgaggt tcaaggaacg ggtccggacg
    10801 gaggtggaca ggccgatgct ggagcgcctg gtgcctgtcg gcacggtcct gcgctccgtg
    10861 tacaccgagc tgcgggaggg gaaggtgact ttcggccggc agatagggac gtaccccctg
    10921 ctggtgggca tcccccaccc ggtggagctg ggaaggttct acgatgtggc ggtggtcggg
    10981 cacggcttcc gcagcataag cgcggtggag tttcccctgg ccgtaaactc ctgttcgatc
    11041 gcagccctgg aggcgctccc tggattaggg cggaagcggg ccgtccgtct gttccgggcg
    11101 cgccccctgg agggccccga cgccgtgcgg agggccttgg acgatccccg ggtggcagat
    11161 gccgtccttc cgttcctgag cttcggccgt cccaacccta attagcgagc aagcggatgc
    11221 agtgcggccc atggagtacc gtgacctagc ccgctacccc ttcctcaagg aggccacgca
    11281 gttcgtcaag gagcgccagc tcgggctgga ggacctcgtc tcctccacct tcgccgccga
    11341 ggccaggagg agggggaagg cccgggtgct caccgccctc agcgacctca ccgtgccgaa
    11401 cggcccggtg acctcggagg aggaggcggc ccaggaggtc ctcagctatc cgtacgcccg
    11461 tctcctcgtg tcatgcattg gcgatgaggc gctggtccgg cgctacgccc tggccgaggc
    11521 caaggccttc aacgagcgcc tgcggaggga cgacatgggc acggtcataa tgctggccgg
    11581 ggagctgggg gtggaggcgg cgcttgccgg ggattccctg cggatgcact tcaccgattt
    11641 tctcggctat acgtccgggc ttcggggccc ggagtggaag ctggtcaacc aggacctccg
    11701 gagggggtac gtcctcctgg agaaggcacg gttcgtgcgc gtgctcgaac aggccttgga
    11761 cgagcggatc gaggacgaac tcccgctgcc ggtgaacgac gagctgctgg aggcggtgcg
    11821 gggggacctg gccgaggtgg tcgcggccct ggaggcctac aaggaacggt accgggacga
    11881 ggggttcggg gaggtgagca tcctgaagtt cccgccctgc atgcggaagc tggtggccat
    11941 ggcgcaggcc gggcagaaca tgccgcacgc cggccgcttc gcccttacca gcttccttag
    12001 cttcatcggg atgccggtgg acgacatcat cggccttttc agcggctccc cggacttcga
    12061 ccgctccaag accgagtatc aggtcaggca catcaccggg gatggggccg ggaagcggta
    12121 cacgcccccg gagtgcgcca ccatgcgcac caacgggctg tgcttcgagc cggacgagct
    12181 gtgcaacggg cggagggtgc ggcatccgct gacctactac cgcatccgct cccatgagaa
    12241 ggccggggag gggaagggcg ctaggccttc ctgagccagg aataagtgag gacggccctc
    12301 tggattgcgg tgaggttccc caccaccgct aaccacagca tcatggcctc gaaggcgttg
    12361 atctcgtacc cgaggagggc gacctcgggc gagcccagca ggaccatgcc cagctgcacg
    12421 agcggcgcga ggaaggagag cacgatgcgg tccgccctcc ccagcagccc ggcgtacatg
    12481 cgcttggcgc ccactgcctg ggcctgggtg cccatgtagc tggtaagcag gacgcccacc
    12541 agcgccatca tcccgaggta gggggagcac caggcgctca ccgcgacccc gccgatcatg
    12601 agcatgtccg cgtagcggtc gaggacgtgg tcaaggaaat cgcccgtccg gtcggccttg
    12661 cccaccagcc tggccacctt tccgtccagg ccgtcgaaga agccgctgac cagcaccacg
    12721 accgcggcca cggggagaag ttcccaatgg gcgtgggaga gatagagcaa cgccccggca
    12781 agggccgcca aggcaagcgc gatcagcgag atgtgattgg gggggacctt gaccatcgcc
    12841 ttggcgaccg ggtccattat gaaatcgacc ttgctgcggt gggagtctag gaccatctca
    12901 acgcctcctc gctccagtcg atattccccg cggcgtactt ctccctttcc ccggccagta
    12961 tggccagcac cgcctccgcc gtctcttcct ggccgagccc ggtggtgtcc acctcgaaca
    13021 ccgggacacc ggagcccatg gcctcggcga ggattatgtc caacgcctcc gcgaggacgt
    13081 tctccctgat cttggccgcc ggccatcccc ttcgctcgag ccggaccgcc agcgcttcgg
    13141 ggcggcagcg caggacgatg accatgtcca catcgaggag gtgcgccaga tggcctacca
    13201 atatgccttc ggcatagcct tcccttccca aggcctcctg gagctcctcg gggtccacct
    13261 catagctgtc ccgggcgggg tccttgcccc tcaacagccc gtgcctcctg gccagctcat
    13321 tgacctccag cacgttccag cccttggtcc gcaccgccgc ggccacggtg ctcttaccgg
    13381 tgccgggggt gccggtcaat gcgactagca tgctacccca tccgataacg catacaaaag
    13441 ggatgccgag ggggcattcc accccctcgg gcccgcggac cttctccatc cgttcgaggg
    13501 cgatgctctt gaaccgcccc gcggtataag aaggggggtg gagcatgcta ccgttacggt
    13561 taaatactat tgttcggggc cttctctgcc agcttgctca tttccacgag cgcctcctcg
    13621 aagagcagac gcacccgcgc ccggtgcgcc ccttcggccg cggcggtggg catccttccc
    13681 gtggggggat aggtccattc cagccctagg tcgacgtcca tcaggaagag cgaggcggga
    13741 tcggcgagcc ccatggccct tcccctgccc tcgagcgcct ccctcagctc cccgagggac
    13801 accttcccct ggcccacctg gtcgagggcg gagacgatgc ggcggaccat gttcctcagg
    13861 aactccctgg cccggaggtc gatgactatg cacccggcca tggggcgcac ctccacggcc
    13921 cccagcgagc ggagggtggc cctgccctcg ggcctgcaga agaggcggaa ctcatgcctg
    13981 ccctcgaact ccttggccgc cgcccgcatc agggcgatgt cctggccccg gtatgggagg
    14041 tggtatctgt accagcggtc cttggcccgg cgggggctga aggcggccgg tacctcggca
    14101 agggcatagc agtgcacgtc cctgcacttg gcgttcaggg cggcgaggag ttccttgggg
    14161 cggaaggagg tgtccaccgc gaagacatta ccgagggcgc tcaccccccg gtcggtgcgg
    14221 ctggccaccc ggaagcggcc cttggccgcc gatccgatgg cgccgatcgc gacgagggcc
    14281 gaaaggagct cgccctccac ggtccgctcg cccggttgcc gctgggagcc catgaagcag
    14341 gtgccatcgt acgcgaactt gaccgccaag cggaccatgg cagggatata ggaacgagcg
    14401 ctaaaggcct ttgcagggcg ggaggacaaa tagataaatg ggggcggcgg tcccggatgc
    14461 gatgccgcag ttcaaggtcg tcctggtcgg accgcgcgtg gacgggaacg tcggggccgt
    14521 cgcccggtcc atggccaact tcggtttcgc cgagctgtgc atggtgcggc cctgcgagct
    14581 cacggaggag gccttccggc gggccaagca cgccggcgac atcctgaggg gggcgcaggt
    14641 ggtccagagc ttcgaggagg cggtggcgga ctgcttccat gtggtgggca cgagcggcaa
    14701 ggtcacgccg ggggagaagc actacatccg catcccgctc tccccgaggg agttcgccga
    14761 gcgggtggag ggcgtggagg agaaggtcgc cctggtcttc gggccggagg acaccgggct
    14821 cacccaggag gagcttgcca ggtgcgacgt gctggtgagc atcccggcgc atgaggacta
    14881 tccggtcctc aacctgtccc atgccgtcgc catcgtcctc tacgagctgt accagagaca
    14941 tatccacatc ggctccccgc ggaaggcggg cgagagcgag cgggagatgc tgcagcggtt
    15001 cttcgatgag ctgctcgacg ccatagatta tcccgagttt cgcagggagc gcacctccgt
    15061 catgttccgg aggatgatgg gcagggccat gcccagcaag tgggagttcc ataccatcat
    15121 gggcgtattc ggggatgcga cgaagatcat caggggccga tgatcagggg ccgtacttcc
    15181 ctaggacctt ccccaggtcg tccccggggg cgacggaggg agggaaccgc tcgctcacct
    15241 cggagatgcc ggtcacgatg gcgatcatgc ggatgttccc ttccatcccc tcgtccacgc
    15301 gcactccgaa cttgacggtg gcgtcctcgt ccagcacctt ggttatgctg gagacgatct
    15361 tgttggcccg cctcatggtc aggttgctgc cgccggagat gtggatgagg gcgcctgtgc
    15421 ccccgcggat gtccacgtcc agcagggggc tgttgatggc gtcgcgcacc accccgtccg
    15481 ggtcggcgtt ctccccatag aggatggttg acacgccccc atgctccatg atcgtcctca
    15541 ggtccgcgaa gtccaggttg atgagcgagg ggaccgtgag gatgtccacc gtgcccttga
    15601 tcagctccga gatcagctgg tcgatgatgg ccagcccctg gtccacgggg aggttctcca
    15661 ccatctgtag gagccggtcg ttgtccagca ccaataggct gttcgaggcc tccctcagcc
    15721 tcttcagccc cttcatcgcg atgttcctgc ggttggcgcc ctcgaagctg aacggggtgg
    15781 tggctatgga gatgaccatg gagccgctgc ggcgggcgat gtccgccacg accggggagg
    15841 ccccggtccc tgtgccaccg ccgaggccgg tgacgatgaa cgtgaggtcc acgtcgtgga
    15901 agagctttga gagcatgggg gcggcgttca gcgcgcactc ctccccgata tcagggcgcc
    15961 cgcctgtgcc cagccccttg gtgtattcca cccctagaag caggcggttc gggcattcgg
    16021 tggccttgag ggacttctgg tcggtattga tggctatggt cgtggctctc tctatgccaa
    16081 gcaggttcag gcggtggatg ctattgcatc cgcccccgcc gcacccaacg accagaatgc
    16141 tgagctcgtg cagctctacc tccggctctt ccagcccctc gaagcgcgcc cccatcccat
    16201 agctcctgcg acgggcggga ggtcaccgca gctcgcccat ggccttgtcc aatcgcatgc
    16261 ggacgtactc gcgcaccgcg ttccgaatgg catcgtcgat ggaaacggaa tcgccctcct
    16321 tgacgaggga ttccagctcc acgaccttca ccttgggcag ctctaccgtc accctttgga
    16381 tgtgctcggg agtgaagttc gtctcgatga aggagtcgat ggccgcccgg atggcgtctg
    16441 atatggtcgg gaacttgccg ccatccacga gcgcctgcag tgctcctagc ttgtccgctt
    16501 ggatccttac ggttatcctc tcgctgtcgc tcatgcccgg tcccgaccta catgtccgac
    16561 aaaaattact tatttgtctg acaaatagac gatatctttt gaacatatat aaaagttggt
    16621 ggttggccag gagcgcgggc tgttcatggc catggtgagg gggaggggga tcgcccgcac
    16681 catgctctct cagcatccct cagcattcta gtatatgtcc ttaccgaaaa cgtttataaa
    16741 ggggtatata aataaccggg aaaggtgacc catttggcca agatacagat agagaa
//
LOCUS       DAOSYN010000009        16573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2893_length_16573_cov_4.190519, whole genome shotgun sequence.
ACCESSION   DAOSYN010000009 DAOSYN010000000
VERSION     DAOSYN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16573
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2893_length_16573_cov_4.190519"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..314
                     /locus_tag="PLJ11_00865"
     CDS             9..314
                     /locus_tag="PLJ11_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOO03256.1"
                     /translation="MIAGLMEPCWVIGLKRSEGLKRLGWSVITIGFVLASMYFLASAM
                     AMGLPMGTAYAVWTGIGAVGALVAGAVLFKERTELVRVFFVLLIIVGIAGVQMTSGA"
     gene            316..684
                     /locus_tag="PLJ11_00870"
     CDS             316..684
                     /locus_tag="PLJ11_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOO03257.1"
                     /translation="MAEEAGERPPLPEGNGRAWATLVVAGLLEPVWVTTMKLSDGFTD
                     IEWAAATFVFLFLSMYMLGKALGMKVPIGTAYAVWVGIGAIGSLVVGMLLFNEPHDLM
                     RLFFMLLIVIGIAGAQATGK"
     gene            complement(695..1918)
                     /locus_tag="PLJ11_00875"
     CDS             complement(695..1918)
                     /locus_tag="PLJ11_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03258.1"
                     /translation="MTEGEREDGPKGADGMADRLLDRVLKGDLRHRLLALVVFGLLLY
                     GAVIAIDGLVLRPFLGDVLSEPTDLNVYRARAENILAGKVPYVDFQSESPPLIMYLFV
                     IPQLAGGSTQAYQLFFAAFAILTSLTLFLFLRKWDERKAMATALLYLAYPLGLMEFGF
                     GVQDEAVTTLLFVLPLLLLLAQRPGLSGATSCAGVLTKMFNVIVLPWMFLRSRDRARL
                     HIVLAFAGIALLLVLPFIVLYPEHLPSFRYYLLGNPEYPTGGSSISPWHYLGKLGYGL
                     PGWAGVALTLTGVGAATLYSYWKDMTLWQGATLVTVVFFLTYPKVLLVYFMMPAVLLL
                     VWGVEDRRVVLRLTAMLVPLFLSVALTGNGMTPIADEPWVWLLGMALSLAGWALMVQA
                     WWAVRERTPFFERMP"
     gene            complement(2106..2489)
                     /locus_tag="PLJ11_00880"
     CDS             complement(2106..2489)
                     /locus_tag="PLJ11_00880"
                     /inference="COORDINATES: protein motif:HMM:NF020334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HOO03259.1"
                     /translation="MPIISISLTEKNLEDLETLQRDLGFGGRSEAIRAAMRTMLAETN
                     ERRSMVGHVDGVLIVVNNTCASGPIHDIYHNNHPLIKTHVHNYLGNDKCMNMMVLSGD
                     ASEINELLDQMHHLEGITYLKFVRS"
     gene            2703..3644
                     /locus_tag="PLJ11_00885"
     CDS             2703..3644
                     /locus_tag="PLJ11_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOO03260.1"
                     /translation="MNRRQLLLVIGSVAMVVALMGAAIASVGAEEDDGRTKVVATFYP
                     LAYMAERIGGERVSVTSLVPYNSELHSWQPAPQDMIRADRADLILYNGGPADAWLMSE
                     VVPGIGADDKLIVNTTVGVQYISGGDEHEGEGGVDPHTWLSPKRALVQALNVYRALCE
                     VDPNGSGYFAERFGALNRSLTLLDAEYEGLSNGSVSGIIISHAAFGYVAYDYGFEQHG
                     AIGLSADQEPSITNMIKLVELMKEEGIYTVLVDPVYNDRYGQVLQAELERKTGSEVTV
                     LSIHLALGPYEDEAGRADYVDQLASNLVSLKAGLGVG"
     gene            3648..4427
                     /locus_tag="PLJ11_00890"
     CDS             3648..4427
                     /locus_tag="PLJ11_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOO03261.1"
                     /translation="MEPIIEVKGLTVRRGPTEALTNVTFEVDRGDYVGIVGPNGGGKT
                     TLVLALLGLLPRASGSIRIMGQDIDTFDQWEKVGYVSQFAINFDPQFPLTVRELVGLG
                     RVNRRNIGRFRTKEDWESVGSALELMGIAELADRRIGTLSGGQRQRAFVAKALVSDPE
                     LLVLDEPVTGFDPVTQERFFMQLANLNQSKGLTILIVSHDLTAVFCRMSRVLCVNREV
                     HGATIVDGTVPDDILKKGYGEHFHFAFHEHKCRGEFADGRP"
     gene            4414..5232
                     /locus_tag="PLJ11_00895"
     CDS             4414..5232
                     /locus_tag="PLJ11_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOO03262.1"
                     /translation="MEGLEILTSMFDYQFVRMALVGGALAAIVSAWIGLFLILRKESM
                     LVDGVAHTAFGGVAVGLLLGFDPMLGAVAVSAAAVLGITYMRRKGMAQSDSAVAVMMA
                     TGFSLGMIIVSLANGFNVDLMTYLFGSILTVSRTDLLVLSALALFIIGFMVFFYKEML
                     AITFDEDGARLQGLPVQAMTVAFNLLVAVTIALSIKVIGIILVVALMVLPGLTALQLG
                     RSFKATVVASTAFGTIAALAGIVLSVLFNVSTSGVIVFTAAGIFLFVAAYRRLG"
     gene            5438..6577
                     /locus_tag="PLJ11_00900"
     CDS             5438..6577
                     /locus_tag="PLJ11_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO03263.1"
                     /translation="MGYEEMFPAMDKRWHDNATKRFASPFVDNPHDRIDVDPIILSAA
                     AVACGYTIRDFYEKPELGIHCVGYISHLYDLLPVTHWFFSLPWVTELGLKLVYKETLP
                     PISTGPIYSAPEDVDKMHVPSKEELVNAQAKYGQLATVPMFYRIYDYVTKNLPMTLVP
                     ISYGFDLVGAAAEMVGVENFIMWTFTEPEAADKLVDKYTQTSINGAEALADKYGSAML
                     IVGSVLANNDIFNDEGVERYSVKNMYKYVDGCFKRGAGPQVFYHLCGNHETDYKLFRD
                     GLMWSPFTVFHIGYKGRNPFPSSLLKQNFQDRATCMGSVDTKLMIDPNPNKVYNQAKE
                     QLLGGRDSKKGYILGTSCETPPYTIPGQMLALVKAARDFGTYGTW"
     gene            6588..7037
                     /locus_tag="PLJ11_00905"
     CDS             6588..7037
                     /locus_tag="PLJ11_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03264.1"
                     /translation="MVKITIPAEVKELIAKRGIKESDVNDVINTAAATKRYIKKGNDG
                     LAKKVIGGLTVYVVSDLSGKGTAVVKTAYAHKMSLGKIVNVMSETDWKTPDGKPIAQG
                     HVEMEYMTVKRNGPALVDPATGDSWVEEYLATKTLAVAEGLFEKKRA"
     gene            7160..8533
                     /locus_tag="PLJ11_00910"
     CDS             7160..8533
                     /locus_tag="PLJ11_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03265.1"
                     /translation="MSKVIGHTNGLCPRCLRTIPVTKVVEDDKVYLVKECPEHGPEKV
                     LIWRGEADYLDMDRYAAVHSKPARIAVQEKDNCPQICGLCPDHVQHTCLVVMEVTNGC
                     NLKCPVCFASSNQFYRFNPSLEVIRGMYQTMLDYIEHPIVVQISGGEPTIRDDLPEIV
                     RMGKEMGIDHIELNTNGIRLAEDIGFLRRLKEAGVDELYFSFDGLHGDIYKQTCGRDI
                     LAQKLRCIENCAEVGMGITLVCVVSPNINLDRVGEVIEFAKSKVPTIKGVHFQPISYF
                     GRFPIAPKDSDRVTIPDLLRAIEDQTKGELRMDNFIPTSCSNVHCDAKSMSVVMEDGS
                     LFPLTSRAFGPPKDTTTVANKTRREIADLWRFIDDSVIFKEEDDGMMNEWGDFVDRAK
                     THYLTVSAMAFQDAWTSETDRFRNCCIHTVTPDGKLIPFCLFNINSMDGRTLYRHEMW
                     SKFSENR"
     gene            complement(8560..8865)
                     /locus_tag="PLJ11_00915"
     CDS             complement(8560..8865)
                     /locus_tag="PLJ11_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03266.1"
                     /translation="MPLTPEMRRAGVTPELMNTTRRFNCPNCGKEFSLMQSRAIACRG
                     CRFANTNCRYARCPYCDKEFPIQQVITKDKYGEKYLAGYMNNILNNYYTQVGKRNSR"
     gene            complement(8942..9718)
                     /locus_tag="PLJ11_00920"
     CDS             complement(8942..9718)
                     /locus_tag="PLJ11_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOO03267.1"
                     /translation="MVNPEMMERTEEFRTLLNLSTYPVGVKLFERASEALTVKGARVL
                     RATAPCHMAALARYYRRDGVTVATAQGMRCLWGLACLGLIATPERLARGDLNLPFTRD
                     EAAARALQANILMLGDEGHLFNALLVAPLDLMPVEPDAIVAYMTPGQALKVLLGFEHG
                     EGRALKGTTLTGQCTVCSAIARVMRGEDLVIDIPCAGDRTWGLVPDQELVVVLSPGTF
                     DDLLAGMRATDAFASHPFRPFLDWPVIFPPEMEPRPSELE"
     gene            9795..11246
                     /locus_tag="PLJ11_00925"
     CDS             9795..11246
                     /locus_tag="PLJ11_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO03268.1"
                     /translation="MSLKERIIVAYARTKLSSRKVSENVENPLHDWVELKVRNTFKHD
                     EGFRKALGRATLGDIDRRTFEEYQLYRFRRQMAYVMENSPYYQKAYAEAGVRPEDIRT
                     MDDLDKVPLTDPNALAEEPFLFLCLSQSKIARTFSTSGTSGKPKRLFYTNDDLLNIVD
                     SIAAALRSAGLKSDETLHIMFPAVVAWDPSLMLESACKVAGLRSVVCSDVNVEEQMRV
                     MREQQARMIIGLTSFIYRVTVLARDRFDLRALGLKAIICSSEPLSEAVRHELEDAWGC
                     KCLSQYGLTEMGLATTIECFVQQGLHINEADFMVEVVDPATGKRLPPGEEGELVWTAL
                     SFQGSPLLRYRSCDISHYILPPCECGHAIIGKIGKPKGRLNDATKIGLGEHIFPALFD
                     EAIMKVHGALSYQLIIDKPSYRDHLHFIVEYNGDLEKGKEEVLKAITGLEEIRSGLEN
                     DLIDPIEVEVREVPHDFTPKRRPIIDRRKRFDA"
     gene            11286..11876
                     /gene="pdxT"
                     /locus_tag="PLJ11_00930"
     CDS             11286..11876
                     /gene="pdxT"
                     /locus_tag="PLJ11_00930"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOO03269.1"
                     /translation="MRVGVISVQGAVPEHIRMSEAALRAMGRKGEVIAVRRLDDLRAV
                     DCLIVPGGESTTISKLLRKLGLFDEVVRMGNEGTPMMGTCAGCVLLAKEGGEQAERTG
                     TELLSLMDMAVDRNAFGRQRESFEAPLHIEGLDLPFPGVFIRAPLITRVWGGCRVVCV
                     HQGEAVMARQGSLLALSFHPELTEDPRLHQMLFELV"
     gene            complement(11880..12620)
                     /locus_tag="PLJ11_00935"
     CDS             complement(11880..12620)
                     /locus_tag="PLJ11_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOO03270.1"
                     /translation="MKVKDYIHERVEGGTMHMTLLDPAKQSPERAAEMASVAEGMGTD
                     AIMVGGSTGVTQENLDATIVAIKRKVKVPVIYFPSGAHAISPYADALYFMSMLNSAAL
                     ENVILQQVAAAPIVKRFGVEPLSMGYIVVEPGMKVGEVGKAKPIPRSEPGTAAAFALA
                     AEYLGMGLVYLEAGSGAPQAVPEEMISLCKRTISIPLIVGGGIRDAETAARVRKAGAD
                     IVVTGTVVEDSDLGEDLRRLIQAAKGTS"
     gene            complement(12617..13432)
                     /locus_tag="PLJ11_00940"
     CDS             complement(12617..13432)
                     /locus_tag="PLJ11_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOO03271.1"
                     /translation="MNPVLQLFRIGNAVMGTVGLLIGVLIAAGTETLDHWQPVLLAAV
                     AVFAFIGAGNSLNDYIDREVDKRAHPERPLPSGRITPRQALSLSAVCFGLSFLVSLAL
                     PLEATAIVALAIVLMLSYEARLKKGALIGNVEIAVLTGMLFLLGGAVVGMMERTYAIA
                     LLAFLAILGREIVKDIEDMEADFDRQTLPKRIGARNAGVIASFAFLAAVALSPLPYIE
                     GTFGIWYLAIVAFADAMFIYCSIIHFQNPTKGQKVAKYAMLLALVAFLVGGML"
     gene            13551..15011
                     /locus_tag="PLJ11_00945"
     CDS             13551..15011
                     /locus_tag="PLJ11_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO03272.1"
                     /translation="MASPELAAEVESIKATVSKYFPVYDVRVSYDSLIFFISPEQSSL
                     RTKFEQLRAEFKPKLLVPILKYSGGEYTLTVVKRPEMRPHGLWLNGALLVITLITTVI
                     AGAVLWASYVGNEDLSLPENYLWGALYFAVPLMAILGTHELCHYLAAKRCGVDASLPF
                     FIPSIPPLGTFGAFISMRDPIPDRRSLVIIGSAGPIGGLLVTIPVSFLGLWLTSMGDP
                     ASGMVGDAGAIAITIQPLYALLAMFIPLPENVTLHPTAFAAWVGFLVTAINLLPAGQL
                     DGGHVARGLAGDKAKYLSYATVALLLVLGLFYMGWLIFAMLILFLGLRHPAPLNDITP
                     LDRRTMLLGVVTLGILLVTFSPIPLVEIAPDHSFDVQLPGGNETVLQAGTSTYFYMHV
                     NNTGNTNTTMRMNVMQVPAGWSASLFLEGEPEANATDLLEVMVPYDEGVTVVLKVSVP
                     PSARTGTWELLVDIRSFNSNGSVYQSEECVFTVTVE"
     gene            complement(14998..16005)
                     /locus_tag="PLJ11_00950"
     CDS             complement(14998..16005)
                     /locus_tag="PLJ11_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO03273.1"
                     /translation="MNAPMGSLPMLLELSGEHPTLPTAEALACLRAEAPGFHQIGSGP
                     GYLIVKGDAGRMGRAVDRLALTHRAGHYLGSCGLDALERTAAGLDLPPGSVCVRVRAV
                     QGLYPEVDTYALARKVGNALSSAHAIDLTSPAMYVRVLLSDEAHLFLVEREIDRRSFE
                     RRKVAERPFFSPISLHPRYARALINLTEARAGDRLLDPFCGTGGIVIEAALMGMKAIG
                     SDIDPAMVDGCRRNLRHFGLEAEVEVADVGDVPEMFGEVAAVATDPPYGRAASTRKED
                     LDTLYSRGMRSMARTLAPGGRAGVVLPIEAGAEGLELQELHRQRVHRSLTRHYHVFIR
                     R"
     gene            complement(16002..>16573)
                     /gene="radA"
                     /locus_tag="PLJ11_00955"
     CDS             complement(16002..>16573)
                     /gene="radA"
                     /locus_tag="PLJ11_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HOO03274.1"
                     /translation="TLPKEKGGLDGDVVMIDTENTFRPERIVQIAKARDADPEEVLKR
                     IHVARAYNSSHQMLLVEKAAEICKKTPVRLMIVDSLTAHFRAEFVGRGTLAERQGLLN
                     KHMHELLSFAEVNNSVIAVTNQVSSKPDAFFGDPTRPVGGHVVAHASTYRLYLRKSKG
                     NRRIARLIDSPDRPEAEAVFTVSEEGIRD"
BASE COUNT         3183 a         5158 c         5193 g         3039 t
ORIGIN      
        1 cctatctcat catcgcagga ctgatggagc cctgctgggt catcgggctc aagcggtccg
       61 aggggctcaa gaggctggga tggagcgtga tcaccatcgg attcgtcctc gccagcatgt
      121 acttcctagc ctcggccatg gccatgggcc tgcccatggg cacggcctat gctgtctgga
      181 ccggcatagg ggcggtcggc gccctggtcg ccggggcggt gctgttcaag gaaaggaccg
      241 agctggtcag ggtcttcttc gttctgttga taatagtggg gatagccggc gtccagatga
      301 cctcgggggc gtgacatggc cgaagaggcg ggggaaaggc cgcccctccc ggaggggaat
      361 gggagggcat gggcaacgct ggtggtggcc gggctgctgg agccagtctg ggtgacgaca
      421 atgaagctct ccgatggatt caccgatatc gaatgggcgg ccgccacctt cgtcttcctg
      481 ttcctcagta tgtacatgct cggcaaggcc ttggggatga aggtccccat aggcacggcc
      541 tacgcggtct gggtggggat aggggccatc ggctccctgg tagtggggat gctgctcttc
      601 aacgagcctc atgacctgat gcgcctgttc ttcatgctcc tcatcgtgat cggaatagca
      661 ggagcacagg cgacgggaaa atgaggcatc ccccctaggg catgcgctcg aagaacggcg
      721 tgcgctccct caccgcccac caggcctgga ccatcaacgc ccaccccgca aggctgaggg
      781 ccatgcccag gagccatacc cacggctcgt ccgcaatggg ggtcatgccg ttcccggtca
      841 gcgccacgga gaggaagagg ggcaccagca tggcggtgag cctgagcacg acccggcggt
      901 cctccacccc ccacacaaga aggagcacag cgggcatcat gaagtacacc agcaggacct
      961 tcggataggt caggaagaaa acgacggtga cgagcgtggc cccctgccaa agggtcatgt
     1021 ccttccagta ggaatacagc gtcgccgccc ccacccccgt cagggtaagg gccaccccgg
     1081 cccatccggg aaggccgtac cccagcttgc ccaggtaatg ccacgggctt atggaagacc
     1141 caccggtcgg atattccgga ttcccgagaa ggtagtaacg gaacgagggc aggtgctcgg
     1201 gatagagcac gatgaacggg agcaccagca gcagggcgat gcccgcgaag gccaggacga
     1261 tatgcagcct ggcgcggtcc ctggagcgaa ggaacatcca gggaaggacg atcacgttga
     1321 acatcttggt cagcaccccg gcgcaggagg ttgccccgga gagcccgggg cgctgggcta
     1381 ataacaatag caacgggagc acgaacagga gcgtggtcac ggcctcgtcc tgcaccccga
     1441 acccgaactc catcagcccc agcggatacg ccaggtagag aagggcggtg gccatcgcct
     1501 tccgctcgtc ccatttccta aggaacagga acaacgttaa ggaggtcaaa atggcgaagg
     1561 cggcaaagaa cagctgatag gcctgagtgg aaccccccgc cagttgcgga atgacgaaga
     1621 ggtacatgat cagcggaggc gactccgact ggaagtccac ataggggacc ttccccgcca
     1681 ggatgttctc cgcccgggcc ctatagacgt tcaggtcggt cggctcgctc aggacgtccc
     1741 cgaggaacgg gcgcagcacc agtccgtcga tggcgatgac cgccccatag agcaggaggc
     1801 cgaacacgac cagggcaagc aatcggtgcc gcaggtcgcc cttcaacacc cggtcaagga
     1861 gccggtcggc catgccgtcc gctcctttcg gcccatcctc ccgctccccc tccgtcatcc
     1921 catcttcctc ccagccccca tcccctggct cgatggggcc taatgtcgtt catacataat
     1981 agatgcgtgt ccctcctcaa gggcaccatc gccccttccg cccactgaaa tggaggatgg
     2041 ccggtcaagg gactagcacc cagataacgg atgccatccg ggtggcgtcc attgcgacgg
     2101 ggcgctcagg agcggacgaa cttcaggtac gtgatccctt ccaggtggtg catctggtcc
     2161 agcagctcgt tgatctccga ggcgtcgcca ctgagcacca tcatgttcat gcacttgtcg
     2221 ttgccgaggt agttgtgtac atgggtcttg atgagcggat ggttgttgtg atagatgtcg
     2281 tggatggggc cggaggcgca ggtgttgttc accacgatca gcaccccgtc cacatggccg
     2341 accatggacc tccgttcatt cgtctctgcg agcatggtgc gcatggccgc ccggatggcc
     2401 tcggagcggc ctccgaagcc caggtccctc tggagtgtct caaggtcctc caggttcttt
     2461 tcggtgaggg agatgctgat gatcggcatg ttgttggcaa tgttattaat cattatatat
     2521 actttttata attgtttaat aatcactgcc cctcgtacga taatgatctc agtgccagta
     2581 ttcatcccga tttcatcact acgcaggaac gatggatcgt gcttctctct ccagttcgac
     2641 cgatttatta acatatccga aaatgttatt aagatgcacc gtattaacgt ctcccgaaga
     2701 aaatgaaccg caggcaactc ctcctcgtca tcggctccgt cgcaatggtc gtggcgctga
     2761 tgggcgcggc catcgccagc gtaggagcag aggaggacga cggacgaacg aaggtcgtcg
     2821 ccaccttcta ccccctggcc tacatggcgg agcgcatcgg cggggaaagg gtcagcgtga
     2881 cgtccctcgt gccgtacaac tccgagctgc actcctggca gcccgcccct caggacatga
     2941 tcagggcgga ccgcgccgat ctgatccttt acaatggagg accggcggac gcatggctga
     3001 tgagtgaggt ggtccccggg attggggccg atgacaagtt gatagtgaac acgacggtcg
     3061 gcgtccaata catctccgga ggggatgagc acgaaggcga agggggcgtc gacccccaca
     3121 cctggctgag tcccaagcgc gccctggtgc aggctctgaa cgtttacagg gcgctctgcg
     3181 aggtggaccc gaacggctca gggtatttcg cagagcggtt cggtgccctg aataggagcc
     3241 tgaccctctt ggatgcggag tatgaggggt tgtcgaacgg cagcgtttcc gggatcatca
     3301 tctcccacgc cgccttcgga tacgtcgcct acgactatgg gttcgaacag catggggcca
     3361 tcggccttag cgcggaccag gagccgtcca tcaccaatat gatcaaactc gttgagctta
     3421 tgaaggagga gggcatctac acggtcctcg tggaccccgt atacaacgac cggtacggac
     3481 aggtgctgca ggccgagctg gagcgcaaga cggggagcga ggtgaccgtg ctgagcatcc
     3541 atctggcgct tgggccgtac gaggacgagg ccggccgtgc cgattacgtc gaccagctgg
     3601 catcgaacct cgtcagtctg aaagcggggc tgggggtggg ataggcaatg gaaccgatca
     3661 tcgaggtgaa gggcctgacc gtccggcgcg ggccgaccga ggccctgacc aatgtcacgt
     3721 tcgaggtgga caggggggac tacgtgggga tcgtgggccc taacggcgga ggcaagacca
     3781 ccctggtgct ggcgctgctg gggctgctgc cccgggcgtc cggcagcata aggatcatgg
     3841 ggcaggacat cgacaccttc gaccaatggg agaaggtggg ctacgtctcc cagttcgcga
     3901 tcaacttcga tccgcagttc cctttgaccg tcagggagct ggtggggctg gggcgggtca
     3961 atcgcaggaa catcggccgg ttccgcacca aggaggactg ggaatcggtc ggctccgccc
     4021 tggaactcat ggggatagcg gagctggcgg acaggcgcat cggcacgctc tcgggcgggc
     4081 agaggcagcg ggccttcgtg gccaaggccc tggtctccga ccccgagctg ctggtgcttg
     4141 acgagccggt caccggcttc gacccggtca cccaggagcg gttcttcatg cagctggcca
     4201 atctcaatca gagcaagggg ctgaccatcc tgatcgtgtc gcacgacctc acggcggtct
     4261 tctgccgcat gtccagggtg ctgtgcgtga acagggaggt gcacggcgcc accatcgtgg
     4321 atgggacggt gccggacgac atattgaaga aggggtacgg ggagcatttc cacttcgcgt
     4381 tccacgagca caaatgcagg ggggagttcg ccgatggaag gccttgagat cctgacatcg
     4441 atgttcgact atcagttcgt ccggatggcc ctggtcggag gggcgctggc ggcgatcgtt
     4501 tccgcctgga tagggctctt tctcatcctt cgaaaggaat ccatgctcgt ggacggggtc
     4561 gcgcacaccg ccttcggcgg ggtggcggtg gggctactgc tcggcttcga tcccatgttg
     4621 ggagcggtgg ccgtctccgc ggcggcggtc ctggggatca cctacatgcg aaggaagggg
     4681 atggcccaat ccgattccgc cgtcgcggtc atgatggcca cgggcttctc cctggggatg
     4741 atcatcgttt ccctggccaa cgggttcaac gtggacctga tgacctacct gttcggttcc
     4801 atcctcaccg tcagccggac ggacctcctg gtcctgagcg cattggccct cttcatcatc
     4861 gggttcatgg tattctttta caaggagatg ctggccatca cgttcgatga ggacggggcg
     4921 aggctgcaag gcctccccgt ccaggccatg accgtggcct tcaacctgct cgtcgcggtg
     4981 acgatcgccc tttccataaa ggtcataggg atcatcctgg tggtggcatt gatggtcctc
     5041 cccgggctga ccgccctgca gctggggcgc tccttcaagg ccacggtggt cgcgtcaacc
     5101 gccttcggga cgatcgcggc cctggcgggc atagtcctgt ccgttctttt caacgtgtcc
     5161 accagcggag tgatcgtctt caccgccgcc gggatcttcc tcttcgtggc ggcctaccgg
     5221 cggttaggat gagcgcgtgc gcggccgacc gctccgcctt ttatcgatgc tttgataaaa
     5281 aattgcggtt ctttgcaaat atttttaatt tgaccgctga aaaatgtaat tgatttctat
     5341 ttcatccttc tataataggc gttaatgaaa cattatttca caggtcttca acaatcatta
     5401 tatatcgtcc atagcagtta acggattggg gatttctatg ggctacgaag aaatgtttcc
     5461 agccatggat aaaaggtggc atgacaatgc caccaagcgt tttgcaagcc ccttcgtgga
     5521 caatccgcac gaccgcatcg acgtggaccc gatcatcttg tccgctgccg ctgtggcttg
     5581 tgggtacacc ataagggact tctatgagaa gcctgagttg ggtatacact gcgtcggcta
     5641 catatcccac ctctatgacc tactgccagt cacgcactgg ttcttctcgc tgccgtgggt
     5701 caccgagctg gggctgaagc tggtctataa ggagacgctg ccgccgatct cgaccggtcc
     5761 gatctactcg gccccggagg atgtcgacaa gatgcatgtg cccagcaagg aagagctagt
     5821 gaacgcgcag gcgaagtatg ggcagttggc gaccgtgccc atgttctaca ggatctacga
     5881 ctatgtgacg aagaacctgc cgatgaccct ggtgcccatc tcctacgggt tcgacctggt
     5941 gggggcggcg gccgagatgg tcggcgtgga gaacttcatc atgtggacgt tcacagagcc
     6001 agaggccgcg gacaagctgg tcgacaagta cacccagacg tcgatcaacg gcgccgaggc
     6061 cctggccgac aagtacgggt cggcgatgct gatcgtcggg tccgtcctgg ccaacaacga
     6121 catcttcaac gatgagggcg tcgagaggta ctccgtgaag aacatgtaca agtacgtcga
     6181 cgggtgcttc aagcggggcg cgggccccca ggtgttctac cacctgtgcg ggaaccatga
     6241 gacggactac aagctgttca gggacggcct gatgtggtcc ccattcaccg tgttccatat
     6301 cggctacaag ggcaggaacc cgttcccgtc cagcctgctg aagcagaact tccaggacag
     6361 ggcgacctgc atggggtccg tcgataccaa gctcatgatc gaccccaacc cgaacaaggt
     6421 gtacaaccag gccaaggagc agctgctcgg cggccgcgac tccaagaagg gctatatcct
     6481 aggtacctct tgtgagactc ctccatacac catacccggg cagatgttgg ccttggtcaa
     6541 ggcggctagg gacttcggta cctacgggac ttggtgaggt gataaaaatg gtaaaaataa
     6601 ctataccggc cgaagtgaag gagctcatcg cgaagagggg cataaaggaa tccgacgtca
     6661 atgacgtcat caacaccgct gccgcgacca agaggtacat caagaagggc aacgacgggc
     6721 tggccaagaa ggtaatcgga ggcctcaccg tatacgtggt cagcgacctg tccggaaagg
     6781 ggaccgccgt ggtcaagacg gcctacgcgc acaagatgtc tctgggcaag atcgtcaacg
     6841 tcatgtccga gaccgattgg aagaccccgg acggcaagcc gatcgcccaa ggccacgtcg
     6901 agatggagta catgaccgtc aagaggaacg gtcccgccct ggtcgacccg gccaccggcg
     6961 actcttgggt ggaggagtac ctcgccacca agaccctggc cgttgcggaa ggtctcttcg
     7021 agaagaagcg cgcttgaaaa catcttcagg ccgctaaacg cggcctttcc ttttcatttc
     7081 gtcccacgat cggcatccat ttttagaaaa agtagaataa tcggccaggc gatcggggtc
     7141 cgtcatcacg gtgaatccaa tgagcaaggt catcggtcat accaacggtc tatgccccag
     7201 gtgcctaagg accatccccg ttaccaaggt ggtcgaggac gacaaggtct acctggtcaa
     7261 ggaatgcccc gagcacgggc cagagaaggt attgatatgg aggggcgagg cggactatct
     7321 ggacatggac cgctatgccg cggtccattc caaaccggcg cgcatagcgg tgcaggagaa
     7381 ggacaactgc ccgcagatct gcgggctctg cccggaccat gtccagcaca catgcctggt
     7441 ggtcatggag gtcaccaacg gctgcaacct caaatgcccg gtgtgcttcg cctcctccaa
     7501 tcagttctac aggttcaacc cctccctgga ggtcatcagg ggcatgtacc agaccatgct
     7561 cgattatatc gagcacccca tcgtggttca gataagcggg ggcgaaccga ccatccggga
     7621 cgacctcccc gagatcgtgc gcatgggcaa ggagatgggt atcgaccaca tcgagctgaa
     7681 cacgaacggc atccgcctgg ccgaggacat cgggttcctg agaaggctga aggaggccgg
     7741 ggtggacgag ctgtacttct ccttcgacgg cctgcacggg gacatataca agcagacctg
     7801 cggccgggac atactggcgc agaagctgcg gtgcatcgag aactgcgcgg aggtcggcat
     7861 ggggatcacc ctcgtgtgcg tggtctcccc gaacataaac ctggaccggg tcggggaggt
     7921 cattgaattc gccaagtcca aggtgccgac catcaagggg gtgcacttcc agccgatatc
     7981 ctacttcggg cgcttcccga tcgccccaaa ggactcggac cgcgtgacca tccccgacct
     8041 gctgagggcg atcgaggacc agaccaaagg cgaactgagg atggacaact tcatcccgac
     8101 ctcatgctcc aacgtccact gcgacgccaa gtccatgtcc gtggtcatgg aggacgggtc
     8161 cctgttcccc ctcaccagcc gcgccttcgg cccccccaag gacaccacca cggtcgccaa
     8221 caagaccagg agggagatcg ccgacctctg gcgcttcatc gacgactccg tgatcttcaa
     8281 ggaggaggac gacggcatga tgaacgaatg gggggatttc gtggacaggg caaagaccca
     8341 ttacctgacg gtctccgcca tggccttcca ggacgcctgg acctcggaga cggacaggtt
     8401 ccgcaactgc tgcatccaca ccgtcacccc cgacggcaag ctcatcccgt tctgcctgtt
     8461 caacatcaac tctatggatg gacggactct ctatcggcac gagatgtggt ccaagttcag
     8521 cgagaacagg tgagggaggg gcgcccctcc taaaaggcct cagcgggagt tcctcttccc
     8581 gacctgcgtg tagtagttgt tcaggatgtt gttcatgtag ccggccaggt acttctcccc
     8641 gtacttgtcc ttggtaatga cctgctggat cgggaactcc ttgtcacagt aggggcagcg
     8701 ggcgtagcgg cagttggtgt tggcgaagcg gcacccccga caggcgatcg cccgggactg
     8761 catgaggctg aactccttcc cgcagttcgg gcaattgaac cttcgcgtcg tgttcatcag
     8821 ttccggcgtt acccccgccc tgcgcatttc cggtgtcagc ggcattctat cacgctggct
     8881 acatatcacc gctcggatat atggcttttc cctcggttta cgtttctata aggaactttc
     8941 gtcattccag ctccgacggg cgcggctcca tctccggcgg gaagatgacc ggccaatcga
     9001 ggaacggcct gaaggggtgg gaggcgaagg cgtccgtggc cctcatccct gccaggaggt
     9061 catcgaacgt cccgggggaa aggacgacca caagctcctg gtcgggcaca agcccccagg
     9121 tgcggtcccc tgcgcacgga atgtcgatca ctagatcctc ccccctcatg acccgggcga
     9181 tcgctgagca gaccgtgcac tgccccgtca acgtggtgcc cttcagcgcc cttccttccc
     9241 cgtgctcgaa ccctagaagg accttgaggg cctgtcccgg ggtcatgtag gccacgatgg
     9301 cgtccggctc cacgggcatg aggtccaggg gggcgaccag caaagcgttg aacaggtgcc
     9361 cctcgtcccc cagcatcagg atgttggcct ggagggccct cgccgccgcc tcatcccggg
     9421 tgaacgggag gttgaggtcg ccccgcgcca accgctcggg ggtggcgatc aggcccaggc
     9481 aggcaaggcc ccaaaggcac cgcatgccct gggcggtggc gacggtcacc ccgtcccgac
     9541 ggtagtacct ggcgagggcg gccatgtggc agggggcggt ggcccggagc accctggcgc
     9601 ccttgaccgt caacgcctcg gaggcccttt cgaacagctt cacccccacg gggtacgtgc
     9661 tcaggttgag gagggttcgg aactcctcgg tccgctccat catctccggg ttgaccatgc
     9721 cccggctatg cgcatgcggc ataatagccc tgcggtttga aaagactata tcggcgctag
     9781 ggcattcgct cccaatgagc ctcaaggaac gcatcatcgt cgcctacgcc cgcaccaagc
     9841 tctcctccag gaaggtctcc gagaacgtgg agaatccatt gcacgattgg gtcgagctca
     9901 aggtgcggaa caccttcaag catgacgagg ggttccgcaa ggccctgggg cgggccaccc
     9961 taggcgatat agatcggcgg acgttcgagg agtaccagct ctacaggttc cggcggcaga
    10021 tggcctacgt catggagaac agcccgtact atcaaaaggc gtacgccgag gccggggtga
    10081 ggccggagga catccggacc atggacgacc tggacaaggt cccgctgacc gacccgaacg
    10141 ctttggcaga ggaaccgttc ctgttcctgt gcctctccca gagcaagata gccaggacct
    10201 tctccacctc gggcacatcc ggcaagccga agcgcctgtt ctacacgaac gacgatctgc
    10261 tgaacatcgt ggactccatc gccgccgccc tgcgctccgc ggggttgaag agcgacgaga
    10321 cgctgcacat aatgttcccg gcggtggtgg cctgggaccc cagcctcatg cttgagagcg
    10381 cctgcaaggt ggcggggctg aggtcggtgg tctgctcgga cgtgaacgtg gaggagcaga
    10441 tgcgggtcat gagggagcag caggccagga tgatcatcgg cctcacctcc ttcatctacc
    10501 gggtgacggt cctggccagg gacaggttcg acctgcgggc gctcggcctc aaggccatta
    10561 tctgctcctc ggagccgctc tccgaggccg tgcgccatga gcttgaggat gcatggggat
    10621 gcaaatgcct gagccaatac ggcctgacgg agatggggct ggccacgacc atcgagtgct
    10681 ttgtacagca ggggctgcac atcaacgagg ccgatttcat ggtggaggtc gtcgaccccg
    10741 cgacagggaa gcgcctgccg cctggggagg agggggagct cgtctggacc gcgctgtcct
    10801 tccagggctc gcccctcctc cgctaccgct cctgcgacat ctcccattac atcctcccgc
    10861 cctgcgagtg cggccacgcg atcatcggca agataggcaa gcccaagggc cgcctgaacg
    10921 acgccaccaa gatcggcctg ggagagcaca tcttcccggc gctgttcgac gaggcgatca
    10981 tgaaggtgca tggcgcgctg agctaccagc tgatcatcga caagccctcc taccgcgacc
    11041 acctccactt catcgtggag tacaacgggg acctcgaaaa ggggaaggag gaggtgctca
    11101 aggccatcac cgggctggag gagatccgca gcggtctgga gaacgacctc atcgacccca
    11161 tcgaggtgga ggtgagggag gtcccgcacg acttcacccc caagcgcagg ccgatcatcg
    11221 accgccggaa gcgcttcgat gcctgagggc aagggttatc tatcgctccc ggaatggccc
    11281 tggggatgag ggtaggcgtc atctccgtcc agggcgcggt cccggagcac attcgcatgt
    11341 ccgaggccgc cctgagggcc atgggcagga agggcgaggt gatcgccgtc cgccgcctgg
    11401 acgacctgcg ggccgtggac tgcctgatag tccccggagg ggagagcacg acgatctcca
    11461 agctcctgcg caagctcggg ctcttcgatg aggtggtccg gatgggcaac gaggggacgc
    11521 cgatgatggg cacctgcgcg ggatgcgtgc tcctggccaa ggagggcggg gagcaggccg
    11581 agcggaccgg cacggagctg ctctcgctga tggacatggc ggtggaccgc aacgccttcg
    11641 ggcggcagcg cgagtcgttc gaggctcccc tgcacatcga aggcctggac ctcccgttcc
    11701 cgggggtctt catccgcgcc ccgctcataa cgagggtctg ggggggctgc cgggtcgtat
    11761 gcgttcatca gggggaggcg gtaatggcca ggcagggcag cctattggcg ctctccttcc
    11821 atcccgaact gacggaggac ccccgcctgc atcagatgct gttcgaactc gtgtaaggct
    11881 caggaggtcc ccttggccgc ctggatgagc ctcctgaggt cctcccccag gtcggagtcc
    11941 tccaccacgg tcccggtgac cacgatgtcc gcccctgcct ttctgaccct ggcggcggtc
    12001 tcggcgtccc tgatgccccc gccgacgatc aaaggtattg agatggtcct cttgcacagg
    12061 gagatcatct cctccgggac ggcctggggg gccccgctgc ccgcctcaag gtacacaagc
    12121 cccatgccca ggtactccgc agcgagggcg aaggcggcgg cggtcccggg ctcggaccta
    12181 gggattggtt tggccttccc cacctccccg accttcatgc ccggctccac gactatatag
    12241 cccatggaca gcggctccac cccgaaccgc ttgacgatgg gggccgcggc cacctgctgc
    12301 aggatgacgt tctccaaggc cgcgctgttc agcatgctca tgaagtacag ggcgtccgcg
    12361 tagggggaga tggcatgcgc accggacggg aagtagatca ccggcacctt gaccttccgc
    12421 ttgatggcca cgatggtggc gtccaggttc tcctgggtca ccccggtgga acccccgacc
    12481 atgatcgcgt ccgtgcccat gccctcggcc acggaggcca tctccgcggc gcgttcgggg
    12541 ctctgcttcg cagggtccag gagcgtcatg tgcatggtcc cgccctctac cctctcatga
    12601 atgtagtcct tgaccttcac aacattcctc ctacgaggaa ggccaccaat gccagcagca
    12661 tggcgtactt ggccaccttc tgccccttgg tggggttctg aaaatgaatt atggagcaat
    12721 atataaacat tgcatccgcg aaggcaacga tggccaggta ccagatgccg aaggtgcctt
    12781 cgatgtacgg aagggggctg agggccacgg cggcgaggaa ggcgaacgag gcgatgacgc
    12841 ccgcgttcct agcaccaatc ctcttcggga gcgtctggcg atcgaagtcg gcctccatgt
    12901 cctcaatgtc cttgacgatc tcccttccca gtatcgccag gaaggccagg agggcgatcg
    12961 cataggtcct ctccatcatg cccaccaccg cccctccgag gaggaacagc atcccggtca
    13021 gcaccgctat ctctacgttc cctatgaggg cacccttctt gagcctggcc tcgtaggaga
    13081 gcatgagcac gatggccaac gcgacgatcg ccgtcgcctc caggggaagc gccagggaga
    13141 ccaggaacga taggccgaag cagacggcgg agaggctgag ggcctgacgc ggggtgatgc
    13201 ggccggacgg caggggcctc tcgggatggg cccgcttgtc gacctccctg tcaatgtaat
    13261 cgttcagcga gttccccgcc cctatgaagg cgaacaccgc gactgccgca aggagcaccg
    13321 gctgccagtg gtccaaggtc tccgtgccgg cggcgatgag cacccctatg agcaatccca
    13381 cggttcccat cacggcgttg ccgatccgaa acagctggag gacgggattc acggccgaac
    13441 atcgcaatgc ccgtactaaa tgattggcgg cgatcggcgg ggccggaccc cggcatccga
    13501 gcccagttga aggaaaattt tataataaca ccttctggaa tagggaaccc atggcgtccc
    13561 ccgagctcgc ggccgaggtg gagagcatca aggccacagt ctccaagtac ttcccggtct
    13621 acgacgtccg ggtgagctac gattctctca tcttcttcat ctcaccggag cagtccagcc
    13681 ttcgcaccaa gttcgagcag ctccgggcag agttcaagcc caagctgctc gtgcctatcc
    13741 tcaagtacag cggtggagag tacacgctga cggtggtgaa gcggccggag atgaggcccc
    13801 acgggctgtg gctcaacggg gcgctcctcg tgatcaccct gatcaccacg gtgatcgccg
    13861 gggccgtgct ctgggcgtcg tacgtgggga acgaggatct ttccctgcct gagaactacc
    13921 tgtggggggc gctgtacttc gccgttccgc tgatggccat attgggcacc cacgagctat
    13981 gccattacct cgcggcgaag cgatgcggcg tggacgcgtc ccttcccttc ttcatcccat
    14041 ccatcccccc gctggggacc ttcggggcgt tcatctcgat gagggacccc attcccgacc
    14101 gcagatcgct cgtcatcatc ggctccgcag ggcctatagg cggactgctg gtcaccattc
    14161 cggtctcctt cctgggcctt tggctgacct cgatgggcga ccccgccagc gggatggtgg
    14221 gggatgccgg tgccatcgcg atcaccatcc agccgctgta cgccctgctg gccatgttca
    14281 tcccccttcc ggagaacgtc accctgcatc ccacggcctt cgcggcctgg gtgggcttcc
    14341 tggtgaccgc aataaacctc cttccggccg gacagctgga cggggggcac gtggcccggg
    14401 ggctggcggg cgacaaggcc aagtacctaa gctacgccac cgtggccctg ctcctggtcc
    14461 tggggctgtt ctacatgggc tggctgatat tcgcaatgct catcctgttc ctggggctgc
    14521 gccaccccgc ccctctcaac gacatcacgc cgttggacag gaggaccatg ctgctgggcg
    14581 tagtgaccct cggcatcctg ctggtcacct tctcccccat ccccttggtg gagatagcgc
    14641 ccgaccactc cttcgacgtt cagctgcccg gagggaacga gacggtgctc caggccggga
    14701 ccagcaccta cttctacatg cacgtgaaca acaccgggaa caccaacacg accatgagga
    14761 tgaacgtgat gcaggtgcct gctggctgga gtgcctcgct cttcctcgag ggcgagcccg
    14821 aagcgaacgc caccgatctc ctggaggtca tggtgcccta tgacgagggt gtgacggtgg
    14881 tcctgaaggt cagcgttccg ccctccgctc gcacgggcac ctgggagctc ctggtggata
    14941 tacgctcgtt caacagcaac ggttcggtct accagagcga ggagtgcgtc ttcacggtca
    15001 ccgtcgaatg aacacgtggt agtgcctggt gagggaccga tgcaccctct gcctgtgcag
    15061 ctcctgcagc tccaggcctt cggccccagc ctcgatgggg agcaccaccc cggcccttcc
    15121 tcccggtgca agggtccggg ccatggagcg cattcccctg gaatataagg tgtcaaggtc
    15181 ctccttcctg gtgctggccg ctcttccgta tggaggatcg gtggccaccg ccgccacctc
    15241 accgaacatc tcagggacgt cccccacgtc cgccacctcg acctccgcct ccaatccgaa
    15301 atgcctcagg ttccgccggc agccgtcgac catggctgga tcgatgtccg acccgatggc
    15361 cttcatcccc atcaaggcgg cctcgatcac gatgcccccg gtgccgcaga acgggtccag
    15421 gagccggtcg cccgccctgg cctcggtcag gttgatgagc gccctggcat agcgggggtg
    15481 aagggagatg ggggagaaga acggccgttc ggccaccttt cgtcgttcga acgatctccg
    15541 gtcgatctcc cgctccacca ggaagagatg ggcctcgtcg gagagaagga ccctcacgta
    15601 catggccgga gaggtcaggt cgatggcgtg ggcggaggaa agggcgttcc ccaccttccg
    15661 ggccaaggcg tacgtgtcca cctctgggta cagtccctgc accgccctga cccgcacgca
    15721 tacgctcccg ggggggaggt ccagcccggc cgcggtccgc tccagggcgt ccaagccgca
    15781 gcttcccaga tagtgccccg ccctgtgggt gagggccagg cgatcgacgg cgcgccccat
    15841 ccgtcccgca tcgcccttaa cgatcaggta accggggccg gagccgatct gatggaagcc
    15901 gggggcctcc gccctgaggc aggccagggc ctccgccgtc ggcagggtgg gatgctcgcc
    15961 cgagagttcg aggagcattg ggaggctgcc catgggggcg ttcaatccct gatgccttcc
    16021 tcggagacgg tgaacaccgc ctcggcctcc ggccggtccg gagaatcgat gaggcgggct
    16081 atccggcggt tccccttgct cttcctgagg tacagacggt atgtggacgc atgcgccacc
    16141 acatgtcccc ccacagggcg ggtgggatcg ccgaagaagg catccggctt cgacgagacc
    16201 tggttcgtca ccgcgatcac ggaattgttg acctcggcaa agctgagcag ctcatgcatg
    16261 tgcttgttca gcagtccctg cctctccgca agcgtgccgc ggcccacgaa ctcggcccgg
    16321 aaatgggccg tgagggaatc cacgatcatc aggcggacag gggtcttctt gcagatctcg
    16381 gcggccttct ccaccaggag catctgatgc gaggagttgt acgccctggc cacatggatg
    16441 cgcttgagga cctcctccgg gtccgcgtcc ctggctttgg cgatctgcac tatcctttcc
    16501 gggcggaagg tgttctcggt gtcgatcatg acgacgtccc cgtccagccc ccccttctcc
    16561 ttgggcaggg tgg
//
LOCUS       DAOSYN010000010        16520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_2911_length_16520_cov_3.780261, whole genome shotgun sequence.
ACCESSION   DAOSYN010000010 DAOSYN010000000
VERSION     DAOSYN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2911_length_16520_cov_3.780261"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLJ11_00960"
     CDS             complement(<1..458)
                     /locus_tag="PLJ11_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO03275.1"
                     /translation="MEKRTAVLAVLIVAIVIVAGLAATMLMGEGDEEDTIYWLPVPPV
                     NQKDQIARGLIDGGVSWEPYCSDSILSGDTHALMWSGEFWPNHPCCVLAANAGFAKNN
                     PDLVARTVAAHMEATDWIVHTIENKDTETENYTALLQMGAAFSLRNTTVV"
     gene            686..1135
                     /locus_tag="PLJ11_00965"
     CDS             686..1135
                     /locus_tag="PLJ11_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO03276.1"
                     /translation="MFELNVMSNTPLTPIEDLQEASLQFLKQIGYISKGYDPKTEVDN
                     IQESIPYRLFTECFLGNMHRVWAVEELSAYLGTTKPTVYRHLNKLKAIDILEDVSIDM
                     DGQRKKGYRIRYGDLSKAWSFTEANVELAMQSYRKTVEHLQRLARGG"
     gene            1138..1398
                     /locus_tag="PLJ11_00970"
     CDS             1138..1398
                     /locus_tag="PLJ11_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03277.1"
                     /translation="MEERCPTRLTKGSRYHLVLGLGGGGTIDVEGEFLGYLQYGSDVA
                     LTMRMDGGGLRAVPSRSVVYLDILDSKEEREEPPKENGVYFG"
     gene            1431..2987
                     /locus_tag="PLJ11_00975"
     CDS             1431..2987
                     /locus_tag="PLJ11_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOO03278.1"
                     /translation="MDRRDEYHQHLLRLLLSGEIRDKDELQRRKVKLSGEYGLAGVPK
                     NSETLALVPPDRLPEVLDILRRKPVRTLSGVAVVAVMTSPAPCPHGRCAYCPGGVENS
                     SPQSYTGKEPAARRASANDFDPYRQVRSRLDQLATIGHHTDKVDLIIMGGTFTSRPQE
                     YREWFVKRCFDAFNSSDSTDLGSAHRLNEGAEHRVIGMTVETRPDSLSSEVGRECMRL
                     GTTRVELGVQILDDDILASVDRGHGVQAVVDATRVAKDLGLKVCYHVMPGLPGSDPER
                     DVRSFRQMFEDARFRPDMVKIYPTLVVKGTRLYEDWRAGRYVPYGTEEAVQVIAEMKR
                     LVPEYVRIQRIQRDIPAPLIEAGVDKGHLRELAKERLASMGEKCRCIRCREVGLLGIT
                     DIEPDDLRERVVRYDASEGREEFISLVLDDWALAGYVRLRKPEAGPALIRELKVFGRM
                     ARIGEHAGEWQHQGLGGRLLGMAEESARADGYAAIKVTSGVGVRSYYASHGYSRALPY
                     MAKGPASDND"
     gene            3019..3789
                     /locus_tag="PLJ11_00980"
     CDS             3019..3789
                     /locus_tag="PLJ11_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03279.1"
                     /translation="MVCTFCGAPSPEGAMVCSACVAKVRGELFLREWMVDPRSDLRAL
                     GSRSACLRIGPSPQGEVLLRKGSDPYLLLERTIQGEDHSHLPTILDQYLAGMGVGLHL
                     MGDEQVPLRPKLKELLGIPERMDLQGERWGRALLRLGNILALSARDMARLPLGDPMRD
                     EVFRERAAQAMGLYRRASAIPELEPIARANLAMLRHWGGEHEAAIAELRAVASSDDAR
                     LQLAKVYWDLGREDEAAEMVASVQDLEGAILRLRRGCP"
     gene            3786..6431
                     /locus_tag="PLJ11_00985"
     CDS             3786..6431
                     /locus_tag="PLJ11_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO03280.1"
                     /translation="MTDLSEKLDRSGRQRLIEAVRRLSPAEYRDLTISLLGGMGVRVQ
                     SAREQQGALLVHGQGEVAYLVLVSRSGFSDPEMVARSLIKDAKEGGRTPVLMTMQELD
                     PGTLNLLEREGVSYADTDRFLSLLERYGLDEQLLVKEDLKVLEDRGEPCLPSVGKLEE
                     LLEEAERSGRKGDGAGAIRLLDQALQIKPLNDSIWLKKASYLLAMGRAAEALTAATRA
                     AELRPGDAMTWYTIALIHNRLEDRERELAAYDNVLRIDPGNAPALLNKGATLYELGRL
                     EWALKVFNDLVHRYPQDPRGWNNRGLALKGMGRAVEAQAAFEKASALDRDFADPLINL
                     ARMREERGDIPGAVEGWKDVLRLVDSRADIWAHLGTCLRALGELDDALTALDRALALD
                     PSMEPVRREREDLVAQMAAARPGPAPVPGGTLDEEVVEEAKNEGSLAVRPFESGPPAE
                     VKEEKALQVPDAQPPAEVSQERTVQPVGTKVLAPGPSATAVAPADEAGRLEPRSEASV
                     PLPFQGSPSAIEVAEGPEARRLVEIPTGERAAEGEGKEGAPAQAPKSPSEWPFEVRLP
                     PLPEGLEARVWQEAALLLAGKEAEKALQVIDGALRQGDDPALLRLKARALLTLDRGEE
                     AIEALKGALRLAPDDARVLLDIEALLHRHGGDASGLLRAAECEEARARLALGLLEGQR
                     YGELARLNVDGAGLPARLSKSLALVRLGRYRDASKLLKTIISEFPAYPEGLNALGVCM
                     RFMGEYDYEQAMHLMQLALEVDPHHSDAMNNIGCTLFAAGRYEAALEALKAAVDEERR
                     PEYLLNLSNVQMAMGDTGSAKDSLTSALKMEESADALYMLGFIAEGEGEHKWALSLYQ
                     DALEKSPGFREAQAGCDRTKLLARK"
     gene            complement(6479..6721)
                     /locus_tag="PLJ11_00990"
     CDS             complement(6479..6721)
                     /locus_tag="PLJ11_00990"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOO03281.1"
                     /translation="MRKFITEMKGKTVMTNDGQILGMIDNFVVDTLTGEINHVLVLPA
                     EEIDSRLFRTDSHGRLVLPFSEMKDVRDVVVMSISR"
     gene            complement(6802..7431)
                     /locus_tag="PLJ11_00995"
     CDS             complement(6802..7431)
                     /locus_tag="PLJ11_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03282.1"
                     /translation="MVKVLRPKDEGKSAAVAAPTSKGKGEEESKAKATGGRAGGKRPP
                     APKRSGGAVDQRAEALRTLSQFYLGEMDLKLRMRQMTITMGKEPPEWMAALEILKDQI
                     INTEHPDLKLRMYQAMADLLAKVGQKEDLFTIQQIIARYNLKSFKDMGFERVEIECAK
                     DACPVCQRMAGKRFRIEEAMETMPIPCHDCTTDRDAATGYCRCRYFAIF"
     gene            complement(7442..9640)
                     /locus_tag="PLJ11_01000"
     CDS             complement(7442..9640)
                     /locus_tag="PLJ11_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HOO03283.1"
                     /translation="MAESVLLRVAKAQSQTEVGMGRARLDMKTRKELGVDVGQAIEII
                     GKKMTVARVFRAQQEDEGKDIIRMDGVLRSNAGVSIGERVTVSKADPQQATKVVLVPK
                     IPHKMQLAMGTGIDDLFRKGLAGRPLVKGDELLVPNFAWGGQLAPFKVVSTVPAGVVI
                     VAEHTEMVVRAEADEEKETYRPSVNYDDIGGLDDVLQRIREMIELPLKHPELFDRLGI
                     DAPKGVLLYGPPGTGKTLLAKAVANESGASFYSIQGPEIMSKWYGGSEEKLREKFEEA
                     AKNAPSIIFIDEIDSIAPKREDVTGEVERRVVAQLLTLMDGMSERGQVIVIGATNRQD
                     AMDPALRRPGRFDREIEVGVPSFQGRKEILQIHTRGMPLGEDVSLDQYAHVTHGFAGA
                     DLAALAREAAMKCLGRFVPTIDIDRAIPAEVLAKMKVTSEDFDAALKEIEPSAMREVM
                     VEVPNVGWGDIGGLEEVKRQLQEVVEMPIDSPEAFKRLGIRPSQGVLLYGPPGTGKTL
                     LAKAVANESNANFISIKGPEIMSKWYGESERALREIFKKARQVAPSIIFLDEIDAVAP
                     RRGAYEASKAYDTLVNELLTSMDGLTAREGVTVIAATNRPDMIDPALLRPGRFDRLIF
                     VPVPDAPARRAILQVHTRAMPLQDVSLEELVSRTEGFVGADLENLCREAAMAALREDR
                     GAETVGMRHFDAALRTVRPSVDKESAKQYEVMSKNIHKARSGIDDLGIFR"
     gene            9730..10593
                     /locus_tag="PLJ11_01005"
     CDS             9730..10593
                     /locus_tag="PLJ11_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOO03284.1"
                     /translation="MRFSAECAPCLLRRVLFQTRLVDPALEDAVMMACVRMVAEGWEP
                     GTGSAALATKVHRLSYELLGTKDPYAKLKAEANQVALSLLPRAEELVHTSDDRLRTAC
                     LVAIAGNVLDFGIGGIEGPQELVHSFDGLVAQGAEPDDTGRMRALLEGAKEVVYLFDN
                     CGEIILDLPLLRELKGIGLKVTGVVKGEPIITDATWEDLAISGADRELDACLTTGTFA
                     IGLDLERAPAGLRDALARADLVIAKGMANFEALAGTDVRPIAHLLRSKCEPVAKAIGA
                     KRDRNVIKVIE"
     gene            10603..11643
                     /locus_tag="PLJ11_01010"
     CDS             10603..11643
                     /locus_tag="PLJ11_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOO03285.1"
                     /translation="MARRVEQAIILAGGEGTRLRPLTSTRPKPLLPVLGVPCIEYVIR
                     AMAAAGVKHIFLACGYRSEDVRNALGRGDRFGVKLELACEREPMGTAGAVKLLEDRLD
                     GTFVVGSGDTMMDAELGPLIDFHLRRGAEATIGLTEVERPEQFGIVGVSAEGRVERFK
                     EKPRPEEVFSRVINAGIYVLEPEVLRMVPPARKYDFAKDLYVDMLARGRGLFAAPING
                     YWRDIGRPGDLYLANMEMASRRPASMAPAGVDAEGPCHFGEGVRASSSRVRRSVIGRA
                     CVLDASLVDGCLLLDRTSVGRGAEVRGCILGEGCRIGAGAMLRDCVLGDGVTVSPGKA
                     LEGTNLEAGAAL"
     gene            11670..12563
                     /locus_tag="PLJ11_01015"
     CDS             11670..12563
                     /locus_tag="PLJ11_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO03286.1"
                     /translation="MSESPRHAQGPLLLAGMLWGTSFVAGKVGVEGTDPYLFTALRSA
                     VAALSLVPMLFLADFDRSLLRSKAVWGISFLNATGLLLQNVGLTYTTASNTVLLVDIN
                     VVIVAVLAALLLRERLDRRTVAGLVLGMAGVGLIATKGDPATLGGGSGLGDLLAFSAG
                     VVWAFYIVLTTKELRKGHRLVPFTSAVIMLTAVLSVPVGLLMAEDRSVGTGGLALAVY
                     LGLFCTTVAFMLYNWGLRALGATQTSIILLVEMVFGLGFSFLLLGERPDPMSALGGAL
                     ILLAIAVISVRGPCSRQRGAA"
     gene            complement(12560..12949)
                     /locus_tag="PLJ11_01020"
     CDS             complement(12560..12949)
                     /locus_tag="PLJ11_01020"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03287.1"
                     /translation="MSYKQFDVYIQDRPGEIARVAEALARNAVNIRGIATDMGGGRPM
                     VHIITDDANSARSALRAAQLEFEEREVIVVSMADRPGELAKLTKKLARAGVNISSLFM
                     LGPLTPLEEVAMTVDRMDEAKKLLEFS"
     gene            13052..13963
                     /locus_tag="PLJ11_01025"
     CDS             13052..13963
                     /locus_tag="PLJ11_01025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO03288.1"
                     /translation="MPHGPRPFAVTVNWNRADDTLECVRSLLQGNPGTEVLVVDNGSQ
                     DGSVPLIRERYPGLEVLENAQNIGYVKGANQGIRRALELGATHVLLINNDAVAHEGMV
                     GELLAAMERHPTAGIVGPRIFYYGTDVMWFNGGHFNHVLGLSTHPLMDRKGDGDAEDR
                     EVGFITGCAMLVKAEVFREVGLFDEAFEIYTEDLDLCLRAKEQGYGVWLVPKAVAEHK
                     VSMSTGVGGTNLMTPYRSYYYARNMLLMVRKRERGVRFATCFLGQTFILLPYYFLLMG
                     VQGTKGSFRQYVRGYLHALADIVEGRA"
     gene            13968..15380
                     /locus_tag="PLJ11_01030"
     CDS             13968..15380
                     /locus_tag="PLJ11_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03289.1"
                     /translation="MARALGSVRRLLAPSPLVLRALIIGLAVRLLLAPWTSYPYDAYP
                     FYGAVVSSLAGTGPYGNVLFTYPPLFSAILFPFFWSVSQFMDPASFGAFVPAMIGVSR
                     ATGMIVPFVTSPAFNLLFKLPIIVGDALVALTLHRAVDRWKGREAADTAFLVWFLNPL
                     VIFVSSVHGQFDALAAYCGLVGTLGFMDRRYLLSGLSLGLGVLLKLFPAYIILGLGVA
                     LLIQLHAALREGKRASALRPALAFLGGGALSLLTIAPLLLRSRSFLDYIMRRGTYSSL
                     GGMNAWFAAPLLSEGDGGGGAGSGVPFNTILLLLGIALALLVAYLVLRKGCEGERPLL
                     LSTAVLTVVMVTQAVTNPQHLLWLFPFLVLLSPTHPRMLPKMYALSILGLLYLLCLQS
                     GFVFLYPLAQFTGLVSVEALNQGIEAFFIGTGGIRELVLSVLGGAGFLVLLTVPLPPR
                     LDLMDRALARIASGREGDEG"
     gene            15370..16332
                     /locus_tag="PLJ11_01035"
     CDS             15370..16332
                     /locus_tag="PLJ11_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03290.1"
                     /translation="MRAEAIVGVLVAALFVSSALMTYGGPQGAPVLEVDGSDGAFHVA
                     YSMPSSAHGYQATIVAVPVVECRTDDVYVLMSGQGGRSESAPANIQGLVDHLSAELRN
                     IRSSSRVVLLDEADLGRYLASGNGTLVISSSIEEGMAGAVEAWVREGGVLVAIGPYGI
                     PFNGQEGSLAISFLPFVFDGALQRTTAYDMGLRTVCPSYAPSTADIVASSGSLLGYAS
                     GDGASNTMGVVPLGLGRVLVMGGPIEPPFLASMEDVFAWDLARCLEAGVQWAAGPVLV
                     EGLNVPSEGLEGSFALSAPGMGVRVSIYNRDDAHALFSSVLVKA"
     gene            complement(16333..>16520)
                     /locus_tag="PLJ11_01040"
     CDS             complement(16333..>16520)
                     /locus_tag="PLJ11_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03291.1"
                     /translation="VIAVSEDDDTVVFTGFSGYLVALYFFYTLLSTNYAVQLWPMFLG
                     FGIGYVWNTLSSAIPEG"
BASE COUNT         2764 a         5258 c         5537 g         2961 t
ORIGIN      
        1 tccaccacgg tcgtattgcg taggctgaag gcggcgccca tctgtaagag ggcggtgtag
       61 ttctcagttt ccgtatcctt gttctcgatg gtatgcacta tccaatcggt cgcctccatg
      121 tgggcggcca cggtccgggc caccaaatcg gggttgttct ttgcaaagcc ggcattagcg
      181 gccaaaacgc aacatggatg gttgggccag aattccccgg accacatcaa tgcgtgggtg
      241 tccccggata atatggaatc ggagcagtac ggttcccagc tcactccccc atcgatgagc
      301 cctcgggcaa tctggtcctt ttggttgacc gggggcacag gcaaccaata gatggtatcc
      361 tcctcgtccc cttcgcccat cagcatcgtt gcggcaaggc cggcgacgat cacgattgct
      421 acgatgagca cggcgaggac cgcggtcctt ttttccattt gaaaatcacc taaaaaatta
      481 aaaaagatga ttatataaag cgtttttgtc cattaaggaa aaattaatcc ttattgtacg
      541 atttaagatg ataattgccc aactgtatcg tgttcatcgg gaaggttcta tcgcttaggc
      601 tttgccacgt tcatttatta atatttaagg acaagtgttt acaaatttta aataatcata
      661 tcttcttatc ctcttcactg aaaatatgtt cgaattgaac gtgatgagca ataccccgtt
      721 gacccccatc gaagaccttc aggaggcctc gctgcagttc ctgaagcaga tcggttacat
      781 ctccaagggg tatgacccga agacggaagt ggacaacatc caggagagca ttccgtaccg
      841 gctcttcacc gagtgcttcc tcgggaacat gcaccgggtg tgggcggtgg aggagctttc
      901 ggcctacctg ggcaccacca agcccaccgt gtaccggcat ctcaacaagc tcaaggccat
      961 cgacatcctg gaggacgtgt ccatcgacat ggatgggcag aggaagaagg gctaccgcat
     1021 ccgctatggg gacctgagca aggcctggag cttcacggag gccaacgtcg agctggcgat
     1081 gcagtcgtac cgcaagacgg tggagcatct ccaacggctt gccagggggg gctgagcatg
     1141 gaggagagat gccctaccag gctaaccaag ggatcccgct accatctcgt ccttgggctg
     1201 ggagggggag gcaccattga tgtggagggg gagttcctcg ggtaccttca gtatgggagc
     1261 gatgtcgccc tcaccatgcg gatggacggg ggagggctga gagcggtccc ctcccgctcc
     1321 gtggtctatc tggacatact cgattccaag gaagagaggg aggagcctcc caaggagaac
     1381 ggcgtgtact tcggctgacc gcagtgcgca ccgcagacca gaggcatcgg atggaccgcc
     1441 gcgatgaata tcatcagcat ctcctgcggc tgctgctctc cggggagata agggacaagg
     1501 atgagctgca gcgtcgcaag gtcaagctga gcggagagta cgggctggca ggggtgccca
     1561 agaactccga gaccctcgcc ctggtccccc cggacaggct gcccgaggtg ctggacatcc
     1621 ttcgccgcaa gccggtccgc accctaagcg gagtggcggt ggtagcagtg atgacctccc
     1681 ccgcgccgtg cccgcacgga aggtgcgcct actgccccgg aggagtggag aacagctccc
     1741 cgcagtccta cactggcaag gagcccgccg cccggcgggc ctcggccaac gacttcgacc
     1801 cctaccggca ggtccgttcc cgcctggacc agctggccac cataggccac cacaccgaca
     1861 aggtcgacct catcataatg ggcggaacgt tcacatcgag gccccaggag taccgggaat
     1921 ggttcgtcaa gcgctgcttc gacgccttca acagctcgga ctcgacggac ctgggatcgg
     1981 cgcatcggct caacgagggg gccgagcacc gggtcatagg catgaccgtg gagacccgcc
     2041 ccgattcgct ctcatccgaa gtgggacggg agtgcatgcg cctggggacc acccgcgtcg
     2101 agctcggcgt ccagatattg gacgacgaca tcctggcctc ggtcgaccgc gggcacgggg
     2161 tgcaggcggt cgtggacgcc acccgcgtcg ccaaggacct ggggctcaag gtctgctacc
     2221 acgtgatgcc cggcctcccc ggctccgacc cggagaggga cgtacgctcc ttccggcaga
     2281 tgttcgagga cgcccgcttc cggccggaca tggtgaagat ctatccgacc ctggtggtga
     2341 agggcaccag gctgtatgag gactggcgcg ctggaaggta tgtcccgtac ggaacggagg
     2401 aggccgtgca ggtgatcgca gagatgaaga ggctggtgcc cgagtacgtc cgcatccaac
     2461 gcatccaaag ggacatccct gcacccctga tcgaggcagg ggtggacaag gggcacctgc
     2521 gcgagctggc gaaggagcgg ctggcctcca tgggggaaaa atgccgttgc atccgctgcc
     2581 gggaggtcgg cctgctcggg atcacggaca tcgagccgga cgacctcagg gaacgggtgg
     2641 tcaggtacga cgcctcggag gggcgggagg agttcatctc cctggtgctt gacgactggg
     2701 ccctggccgg ctacgtccgt ctgaggaagc ccgaggccgg accggcgctc atccgcgagc
     2761 tgaaggtctt cggacgcatg gccaggatag gcgagcatgc gggggaatgg cagcaccagg
     2821 ggctgggcgg aaggctgctg gggatggccg aggagtcggc gagggcggac ggctatgcgg
     2881 ccatcaaggt caccagcggc gtgggcgttc gaagctacta cgcctcccac ggctactcaa
     2941 gggcgctccc gtacatggca aaggggcctg ccagcgacaa tgattaatcc ccgaccgccg
     3001 atatcatccc gggatgctat ggtatgcacg ttctgcggcg ccccttcccc tgaaggggcc
     3061 atggtctgct cggcctgcgt ggcgaaggtg aggggtgagc tctttctcag agaatggatg
     3121 gtcgatccca ggtccgacct gcgcgccctg ggcagccgca gcgcctgtct gcggataggc
     3181 ccgagccccc agggggaggt cctactgcga aaggggagcg acccctatct gctcctggaa
     3241 aggaccatcc agggcgagga ccactcccat cttccgacca tcttggacca atacctcgcg
     3301 gggatggggg tggggctcca cctgatgggc gatgagcagg tccccctccg ccccaagctg
     3361 aaggagctgc taggcatccc ggagaggatg gacctccagg gggagaggtg ggggagggcg
     3421 ctcctgcgcc tggggaacat cctggcattg agcgcgaggg atatggcccg gcttcccctg
     3481 ggcgatccca tgcgcgatga ggtcttccgt gagcgcgccg cccaggctat ggggctctat
     3541 cggagggcgt cggccatccc ggagctggag ccgatcgcca gggccaacct ggccatgctc
     3601 cggcattggg gaggggagca tgaggccgcc atcgccgagc tcagggcggt ggccagctcc
     3661 gacgacgccc gcctccagct ggcgaaggtg tactgggacc tgggcaggga ggacgaggcc
     3721 gcggagatgg tggcctccgt ccaggacctt gagggggcga tcctcaggct gaggagggga
     3781 tgcccatgac agacctgtcc gagaagctgg accggagcgg gaggcagcga ttgatagagg
     3841 cggtgcgcag gctgtccccg gccgagtacc gcgatctgac gatctctctg ctggggggca
     3901 tgggcgtccg ggtccagtcg gcgcgcgagc agcagggtgc gcttctggtc cacgggcagg
     3961 gggaggtcgc ctatctagtg ctcgtgtccc gtagcggctt ctccgacccc gagatggtcg
     4021 cccggtccct gatcaaggat gccaaggagg gcgggcggac gccggtgctc atgaccatgc
     4081 aggagctgga ccccgggacc ctgaacctcc tggagaggga gggggtctcc tatgcggaca
     4141 cggatcgctt cctgtccctg ctggagagat atgggctcga cgagcagctc ctggtcaagg
     4201 aggacctcaa ggtcctggag gaccgcgggg agccatgcct gccctcggtg ggcaagctcg
     4261 aggaactgtt ggaggaggcc gagcggagcg gaaggaaggg ggacggcgcg ggggccatcc
     4321 gcctcctgga ccaggccctg cagatcaagc ccttgaacga ctcgatttgg ctgaagaagg
     4381 ccagctacct cctggcgatg gggagggcgg ccgaggcgct tacggccgcc acccgggccg
     4441 ccgagcttcg ccccggggat gcgatgacct ggtacaccat cgccctcatc cacaaccgcc
     4501 ttgaggacag ggagagggag ctggccgcct acgacaacgt gctgcgcatc gatccgggga
     4561 acgcccccgc cctcctgaac aagggcgcca ccctgtacga actgggccgc ctggaatggg
     4621 ccctgaaggt cttcaacgac ctcgtccatc gctatcccca ggacccccgg ggctggaaca
     4681 accgcgggct ggccctcaag ggcatggggc gagcggtaga ggcgcaggcc gcgttcgaga
     4741 aggcgagcgc tctggaccgg gactttgcgg accccctgat caacctcgcc cgcatgcgcg
     4801 aggagcgggg ggacatccca ggggcggtgg aggggtggaa ggacgtcctg cggctggtcg
     4861 actccagggc agacatctgg gcccacctcg ggacatgcct gcgcgccctc ggggagctgg
     4921 acgacgccct gacagccctg gaccgagccc tagcgctcga tccctcgatg gagccggtca
     4981 ggcgggaaag ggaggacctg gtggcccaaa tggccgcggc ccggccgggc ccggcgcccg
     5041 tgcccggcgg caccttggac gaagaggtcg tcgaggaggc caagaacgag ggttcgctgg
     5101 ccgtgcggcc gttcgagagc ggtccgccgg ccgaggtgaa agaggagaag gcgctccagg
     5161 tccccgatgc gcagccgccc gcggaggtct cccaggaaag gacggtgcag ccggtcggga
     5221 cgaaggtcct cgcccccggt ccaagcgcga cggccgtcgc ccctgccgat gaggccggaa
     5281 ggctggagcc ccggtcggaa gcatcggtcc ccttgccctt ccagggcagc ccttcggcca
     5341 tcgaggtggc cgagggaccg gaggcccgcc ggctcgtgga gattccaacg ggggagaggg
     5401 cagcggaggg ggagggaaag gagggtgccc ccgcccaagc tcccaagtcc ccttcggaat
     5461 ggcccttcga ggtgcgcctg ccccctctcc cggaaggact ggaggcgcgc gtctggcagg
     5521 aggcagcgct tttgttggca ggaaaggagg ctgaaaaggc cctgcaggtg atcgatggcg
     5581 cgctcaggca gggggacgac ccggccctac tgcggctcaa ggcccgcgcc cttttgaccc
     5641 tggaccgggg ggaggaggcc atcgaggcgt tgaagggagc gctgaggctc gcccccgatg
     5701 acgcccgggt cctattggac atcgaggccc tcctccatcg gcatggcggg gacgcctccg
     5761 ggctgctgcg ggcggcggag tgtgaggagg cccgcgcccg cctggccctg ggcctgctcg
     5821 aagggcagag gtatggcgaa ctcgccaggc tgaacgtgga tggggcgggg ctcccggcaa
     5881 ggctctccaa gtccctggcg ctggtccgcc tgggccggta cagggacgcc tcgaagcttc
     5941 tcaagaccat aatatcggag ttcccggcgt acccggaggg cctgaacgcc cttggggtgt
     6001 gcatgcggtt catgggcgag tacgactacg agcaggcaat gcacctcatg cagctggcgc
     6061 tggaggtcga cccccatcat tccgacgcga tgaacaacat cggatgcacg ctcttcgccg
     6121 ccgggaggta cgaggcggcg ctggaggcgc tgaaggccgc ggtggacgag gagcgccgtc
     6181 cggagtacct gctgaacctg agcaacgtgc agatggccat gggggacact ggatcggcca
     6241 aggacagcct gacgtccgcc ctgaagatgg aggagagcgc cgatgcccta tacatgctgg
     6301 gcttcatagc cgagggggag ggggagcaca aatgggccct tagcctgtac caggacgcgt
     6361 tggagaagag ccccgggttc cgggaggcac aggccggttg cgaccgtact aagctcctcg
     6421 cacggaagtg aagatcagca ggcctggcct tcgagaggaa ggcgatggaa agggaacatc
     6481 agcgggagat gctcatgacc acgacgtcgc ggacgtcctt catctcgctg aacgggagca
     6541 ccaggcggcc atggctgtcg gtgcggaaca gacggctgtc gatctcctcg gccgggagca
     6601 ccagcacgtg gttgatctca ccggtgaggg tgtccaccac gaagttgtct atcatgccca
     6661 agatctgacc gtcgttggtc atgaccgtct tccccttcat ctcggtaatg aacttcctca
     6721 ttcgcgcacc ccgaacaaat ggcccgattg actatcgcat gagattgagc ctatttatag
     6781 atttatggag ggcctggagc ctcaaaatat cgcgaagtag cggcagcggc agtagcctgt
     6841 ggcggcgtcc ctatccgtgg tgcagtcgtg gcaggggatg ggcatggtct ccatggcctc
     6901 ctctatcctg aagcgcttcc ccgccatcct ctgacagacg gggcaggcgt ccttggcgca
     6961 ctcgatctcc acccgctcga accccatgtc cttgaaggac ttgaggttgt agcgggcgat
     7021 gatctgctga atggtgaaaa ggtcctcctt ctgccccacc ttcgccaata ggtccgccat
     7081 tgcctggtac atcctgagct tgaggtcggg atgctccgtg tttatgatct ggtccttcag
     7141 gatctccaag gcggccatcc attccggggg ctccttcccc atggtgatgg tcatctgcct
     7201 catccgtagc ttgaggtcca tctcccccag gtagaactgg gaaagcgtgc gcagggcctc
     7261 tgccctctgg tcgaccgccc ccccgctccg ctttggcgcg gggggcctct tcccccccgc
     7321 ccttccgccc gtggcctttg ccttgctctc ttcctccccc ttccctttgg aggtcggcgc
     7381 ggccacggcc gcgctcttcc cctcgtcctt gggtctcaat accttcacca tctgctagca
     7441 cctacctgaa tatcccgagg tcatcgatcc cgctccgggc cttgtgtatg ttcttgctca
     7501 tgacctcgta ctgcttcgcc gactccttgt ccacggacgg ccggaccgtt ctgagggccg
     7561 catcgaagtg cctcatcccg accgtctccg cgccgcggtc ctccctcagg gcggccatcg
     7621 ccgcctcccg gcacaggttc tcaaggtccg cgcccacgaa cccctcggtg cgggagacca
     7681 gctcctccag gctcacgtcc tggaggggca tggccctggt atgcacctgc aggatggccc
     7741 tacgggcagg tgcatcgggc actggcacga atatgagccg gtcgaagcgc ccggggcgca
     7801 gcagcgccgg gtcgatcatg tccggccggt tggtggcggc gatgaccgtc accccctccc
     7861 gggcggtgag cccgtccatc gatgtcagca gctcattgac cagcgtgtcg tacgccttgg
     7921 aagcctcata ggccccccgg cggggggcga ccgcgtcgat ctcgtccagg aagattatcg
     7981 aaggggccac ctggcgtgcc ttcttgaaga tctcccgcag ggcccgttcg ctctccccgt
     8041 accatttgga catgatctcc gggcccttta tgctgatgaa gttggcgttg gactcgttgg
     8101 ccacggcctt ggccagcagc gtctttcccg ttcccggtgg cccgtagagg agcacgccct
     8161 gcgagggcct tatccccaat cgtttgaagg cctccgggct gtctatgggc atctccacca
     8221 cctcctggag ctggcgcttc acctcctcca gtcccccgat gtccccccag cctacgttgg
     8281 gaacctccac catgacctcg cgcatggccg agggctcgat ctccttcaac gccgcgtcga
     8341 agtcctcgga ggtgaccttc atcttggcca gcacctcggc cggaatggcc cggtcgatgt
     8401 cgatggtggg gacgaagcgt cccaggcact tcatggcggc ctccctggcc agggcggcca
     8461 ggtccgcccc cgcgaacccg tgggtgacat gggcgtactg gtccaggctc acgtcctcgc
     8521 cgaggggcat cccgcgggta tggatctgga ggatctcctt gcggccctgg aaggacggca
     8581 cccccacctc gatctcccgg tcgaacctcc cggggcggcg cagcgcgggg tccatggcgt
     8641 cctggcggtt ggtggccccg atcacaatga cctgccccct ctccgacatc ccgtccatga
     8701 gggtgagcag ctgggcgacc acccttctct ccacctcccc ggtgacatcc tcccgcttgg
     8761 gggcgatcga atctatctcg tcgatgaaga tgatggacgg ggcgttcttg gccgcctcct
     8821 cgaacttctc ccgcagcttc tcctcgctcc ccccgtacca tttggacatt atctccggcc
     8881 cctggatgga atagaaggag gcgccggact cgttggcaac ggccttggcc agcagcgtct
     8941 tgcccgttcc cggcggcccg tagagcagga cgcccttcgg ggcgtctatc cccagccggt
     9001 cgaacagttc gggatgcttg agcggcagct cgatcatctc cctgatgcgc tgcaggacat
     9061 cgtccaggcc gcctatgtca tcgtagttga ccgagggtct gtacgtctcc ttctcctcat
     9121 ctgcctcggc cctcaccacc atctcggtgt gctcggccac tatgaccacg cccgccggga
     9181 cggtggagac caccttgaac ggggcgagct gcccgcccca ggcgaagttg gggaccaaca
     9241 gctcgtcgcc cttgacgagc gggcggccgg cgagcccctt gcggaacagg tcgtctatgc
     9301 cagttcccat cgccagctgc atcttatggg ggatcttagg gacgagcacc accttggtgg
     9361 cctgctgcgg gtcggccttg gagacggtca cccgctcccc tatggatacg cccgcattcg
     9421 agcgcagcac cccgtccatg cggatgatgt ccttgccctc gtcctcctgc tgggcccgga
     9481 agacccgggc gaccgtcatc ttcttaccga ttatctcgat ggcctgcccc acgtccactc
     9541 ccagctcctt gcgggtcttc atgtccaggc gggctcggcc cattcccacc tccgtctggg
     9601 actgggcctt cgcaacacgg aggagaacgg attcggccat tgctatcagg gtcgaaatcc
     9661 cttctctgga tattaagctt ccatcggtcg gacgaaagga ataatagccg tcccctcctt
     9721 ccctcccgca tgaggttctc cgctgaatgc gccccctgcc tgctgcgcag ggtcctgttc
     9781 cagacaaggc tggtggaccc cgcgctcgag gacgctgtca tgatggcgtg cgtccgcatg
     9841 gtcgcggagg gatgggagcc cggaacgggg tcggccgccc ttgccacaaa ggtgcaccgg
     9901 ctctcgtacg aactgctcgg gaccaaggat ccctacgcca agctgaaggc ggaggcgaac
     9961 caggtggccc tctccctcct gccccgggcg gaggaactcg tccacacctc ggacgacagg
    10021 ctgcgaacgg cgtgcctcgt ggccatagcc ggcaacgtcc tggacttcgg catagggggg
    10081 atcgagggcc cccaggaact ggtccactcc ttcgatgggc ttgtggccca gggagcggag
    10141 ccggacgata cggggcgcat gcgcgcgcta ttggaagggg cgaaggaggt cgtgtacctc
    10201 ttcgacaact gtggcgagat catcctggac ctcccgctcc tccgggagtt gaagggcata
    10261 ggcctgaagg tcacaggggt ggtgaagggg gaaccgataa tcaccgatgc cacctgggag
    10321 gacctggcga tctccggggc cgacagggag ctggacgcgt gcttgacaac ggggaccttc
    10381 gccatcggac tcgacctgga gcgggcccca gccgggctga gggacgccct ggcaagggcg
    10441 gacctcgtca tcgccaaggg catggccaac ttcgaggcat tggccggcac cgatgtaagg
    10501 ccgatagcgc acctgttgcg gagcaaatgc gagccggtgg ccaaggccat cggggccaag
    10561 agggaccgca acgtcattaa ggtcatcgag tgagggggac gaatggcacg gagggtggag
    10621 caggcgatca ttctggctgg gggggagggg accaggctga gaccattgac ctccacccgc
    10681 ccgaagccac tgctgccggt gctgggcgtt ccctgcatcg agtacgtgat cagggcaatg
    10741 gcggcggccg gcgtcaagca catcttcctg gcctgcggct accgctcgga ggatgtgcgg
    10801 aacgcgctgg ggagagggga ccgcttcggg gtgaagctcg agctcgcctg cgagagggag
    10861 ccgatgggca cggcgggggc ggtgaagctg ctggaggacc gcctggacgg taccttcgtc
    10921 gtggggagcg gggacacgat gatggacgcc gagctcgggc cgctgatcga cttccatctg
    10981 cggagggggg cggaggccac cattgggttg acggaggtgg agcggccgga gcagttcggc
    11041 atcgtcggcg tctcagcgga gggaagggtg gagcgcttca aggagaagcc ccgccccgag
    11101 gaggtcttct cccgggtgat caacgccggc atctacgtcc tggaacccga ggtgctgcgg
    11161 atggtccctc cggcacggaa gtacgacttc gcgaaggacc tgtacgtgga catgctggcg
    11221 aggggtcgcg gccttttcgc cgcccccatc aacggctatt ggagggacat aggccgcccg
    11281 ggcgacctct atctggccaa tatggagatg gcctcccgca ggcccgcgtc catggcccca
    11341 gcgggcgtgg acgcggaggg tccatgtcac ttcggcgaag gggtgagggc gtcctcctcc
    11401 agggtgagga ggagcgtcat aggccgcgcc tgcgtcctgg acgcctcgct cgttgacggc
    11461 tgcctcctcc tggaccggac atcggtcggc aggggggcgg aggtgcgcgg ctgcatcctg
    11521 ggggagggct gccgcatcgg tgcgggcgcg atgctcaggg actgcgtgct cggggacggt
    11581 gtaaccgtat cgcccggcaa agcccttgaa ggaacgaacc tggaggcagg ggcggcgctc
    11641 tgaaatacgc ggaaggggtt cccccgccca tgtccgaaag cccccgccac gcccaaggac
    11701 cgctgttgct ggcggggatg ctctggggca cctccttcgt ggccgggaag gtgggcgtgg
    11761 agggcactga cccctacctc ttcaccgccc tgcggtccgc ggtggccgcc ctgtccctgg
    11821 tccccatgct ctttctggca gacttcgacc gctcccttct ccggagcaag gccgtctggg
    11881 ggatctcgtt cttgaacgct acggggctcc tcctgcagaa cgtcggcctc acctacacga
    11941 cggcgagcaa caccgtgctc ctcgtggaca tcaacgtggt catcgtggcg gtccttgccg
    12001 ccctgctgct gagagagcgg ttggaccgcc ggaccgtggc cgggctggtc ctgggcatgg
    12061 ctggggtggg cctcatcgcc acgaaggggg accccgccac cttgggaggg gggagcgggc
    12121 tcggggacct gctggcgttc tcagccgggg tggtgtgggc cttctacatc gtcctcacca
    12181 cgaaggagct gaggaaaggg cataggctcg tgcccttcac ctcggccgtg atcatgctca
    12241 ccgcggtcct ctccgtgccc gtggggctgc tcatggccga ggaccgctcg gtgggaacgg
    12301 gcggattggc attggcggtg tacctggggc tgttctgcac cacggtggcc ttcatgctct
    12361 ataactgggg gttgagggcc ctgggggcga cccaaacctc gatcatcctg ctcgtggaga
    12421 tggtgttcgg cctgggcttc tccttcctcc tgctgggcga gaggccggac cccatgtctg
    12481 cgctgggcgg cgccctcatc ctcctggcga tcgcggtgat ctccgtcagg ggcccatgct
    12541 ccaggcagag gggcgcggcc taggagaact ccagcagctt cttggcctca tccatccgat
    12601 ccacggtcat ggccacctcc tccagggggg tgagggggcc cagcatgaac agggagctga
    12661 tgttcacccc ggcccgggcg agcttcttgg tcagcttggc aagctccccg ggccgatccg
    12721 ccatggagac cacgatgacc tcgcgctcct cgaactccaa ctgcgccgcc ctcaaggcgc
    12781 tccgggcgct gttagcgtcg tcggtgatta tgtgcaccat cggcctcccc ccgcccatgt
    12841 ccgttgctat tccacggatg ttgacggcgt tccgggcaag cgcctccgcc acccgcgcga
    12901 tctcccccgg acggtcctgt atatacacgt cgaactgctt gtaggacatc gctttcagat
    12961 tgatggtgtg ggcaattaat actttgtgag gaagcgccga tggggaggcg ccgaaggatt
    13021 agatttatag cgcgacccca tacgtagggg catgccccac ggccccaggc ccttcgccgt
    13081 tacggtgaac tggaaccggg cggacgacac cctggaatgc gtccgttcgc tgctccaggg
    13141 caatcctgga acggaggtcc tggtggtgga caacggctcg caggacggct cggtccccct
    13201 catcagggag aggtatccgg ggctcgaggt gctggagaac gcgcagaaca tcggctatgt
    13261 caagggcgcg aaccagggga tacgccgggc ccttgagctg ggggccaccc acgtgctcct
    13321 catcaacaac gatgcggtcg cccacgaggg gatggtgggc gagctgctcg cggccatgga
    13381 gaggcatccg acggccggga tcgtcggtcc gaggatattc tattacggca cggacgtgat
    13441 gtggttcaac ggtgggcatt tcaaccacgt gctggggctg tccacccatc cgctcatgga
    13501 ccgcaagggc gacggcgatg cagaggaccg cgaggtgggg ttcatcaccg gctgcgccat
    13561 gctggtcaag gccgaggtct tccgcgaggt gggcctgttc gacgaggcct tcgagatcta
    13621 cacggaggac ctggacctgt gcctgagggc gaaggagcag gggtacggcg tgtggctggt
    13681 gcccaaggcc gtggccgagc ataaggtgtc catgtctacg ggtgtgggcg gcacgaacct
    13741 tatgaccccc taccggtcgt actattacgc ccggaacatg ctgctgatgg tccggaagag
    13801 ggagaggggg gtgcgcttcg ccacctgctt cctggggcag acgttcatcc tcctccccta
    13861 ttacttcctg ctcatggggg tgcaggggac caaggggtcc ttcaggcagt acgtgagagg
    13921 gtacctccac gccctagcgg acatcgtgga agggagggca tgagcccatg gcccgagcgc
    13981 tcgggagcgt ccggcgcctc ctagccccct cccccctggt gctccgggcc ctcatcatcg
    14041 ggctggcggt aaggctcctg ctggcgccct ggacctccta cccctacgac gcctatccgt
    14101 tctacggggc cgtggtcagt tccctggcag gcacaggccc ctatgggaac gtgctcttca
    14161 cctacccccc gctgttctcc gcgatcctct ttcccttctt ctggtccgtc tcccagttca
    14221 tggaccccgc ctccttcggc gccttcgtcc cggccatgat cggcgtctcc cgggccacgg
    14281 ggatgatcgt gcccttcgtc acctccccgg ccttcaatct tcttttcaag ctgccgatca
    14341 tcgtcgggga tgcgctcgtc gccctcaccc tgcaccgcgc ggtggaccgc tggaagggca
    14401 gggaggccgc ggacacggca ttcctggtat ggttcctgaa tcccctggtc atcttcgtga
    14461 gttcggtgca cgggcagttc gacgcgctcg ccgcctactg cggcctggtg ggaacgctcg
    14521 ggttcatgga ccgccggtac cttctgagcg gcctctcctt ggggctgggc gtgctgctca
    14581 agctgttccc cgcctacatc atcctggggc tgggggtagc cctgctgatc cagctgcacg
    14641 cagcacttcg cgaagggaag agggcctccg ccctcaggcc tgccctggcg ttcctgggcg
    14701 gaggggcgct cagcctcctc accatagcgc ccctgctgct cagatcccgc tccttcctgg
    14761 attacataat gagacggggg acctactcgt ccctgggcgg gatgaacgcc tggttcgccg
    14821 ccccgttgct tagcgagggg gacggcgggg gcggggcagg cagcggggtg ccgttcaaca
    14881 cgatcctcct gcttctgggg atcgcgctcg cgctcctcgt ggcgtacctt gtgctcagga
    14941 aggggtgcga gggtgaacgg ccattgcttc tctccacggc cgtcctaacc gtggtgatgg
    15001 tcacccaggc ggtcacgaac ccgcagcacc tcctctggct cttcccgttc ctggtcctgc
    15061 tctcgcccac gcacccccgc atgctcccca agatgtacgc cctctccatc ctgggcctgc
    15121 tctatctcct ctgcctgcag tcggggttcg tcttccttta tccgctcgcg cagttcactg
    15181 ggctggtctc ggtggaggcg ctgaaccagg ggatcgaggc gttcttcatt gggacggggg
    15241 ggataaggga actggtcctg agcgtcctgg gcggcgccgg gttccttgtt ctcctgacag
    15301 tgcccctgcc ccctcggttg gacctcatgg accgcgctct cgcaaggatc gcctcaggga
    15361 gggagggcga tgagggctga ggcgatcgtg ggcgtgctcg tggcggccct gttcgtgtcc
    15421 tcggccctca tgacctatgg aggcccccaa ggcgcccccg tcttggaggt ggacgggtcg
    15481 gacggagcct tccacgttgc ctattctatg ccatcctcgg cccatgggta ccaggcgacg
    15541 atcgtggccg tgcccgtggt ggaatgcagg accgacgacg tatatgtgct catgtccggc
    15601 caaggcgggc ggtccgagag cgccccggcc aacatccagg ggctcgtcga tcatctctcc
    15661 gccgagctga ggaacatccg ttcgtcctca agggtcgtac tgctcgacga ggccgacctc
    15721 ggccgctacc tggcctcggg caatggcacc ttggtcatct ccagctccat cgaggagggc
    15781 atggccgggg cggtggaggc ctgggtcagg gagggagggg tcctggtggc catcggtccc
    15841 tacggcatcc cgttcaacgg ccaggagggc tccctggcca tcagcttcct gcctttcgta
    15901 tttgatgggg cactccaacg gacgacggcc tacgacatgg ggctgaggac cgtctgtcct
    15961 tcgtacgccc cctccaccgc cgatatcgtc gcctcctccg gctccctcct cgggtacgcg
    16021 tccggggacg gggcgtccaa caccatgggc gtggtccccc tcggactggg gcgcgtgctg
    16081 gttatgggcg gccccatcga accccccttc ctggccagca tggaggatgt attcgcctgg
    16141 gacctggcgc gatgcttgga ggcgggggtg cagtgggccg cggggccggt cctcgtggaa
    16201 ggactgaacg tcccctccga aggcctggag ggctcgttcg ccctgagcgc cccggggatg
    16261 ggcgtgaggg tatccatcta caaccgggac gacgcgcacg cccttttcag cagcgttctc
    16321 gtcaaggcct gatcaacctt ccgggattgc gctgctgagc gtgttccata catagccgat
    16381 accgaacccg aggaacatcg gccaaagctg cacggcgtaa ttggtggaga gcagcgtgta
    16441 gaagaaatag agcgcgacca ggtagccgga gaagccggtg aagaccaccg tgtcgtcgtc
    16501 ctcgctgacg gcgatgaccg
//
LOCUS       DAOSYN010000011        16048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_3034_length_16048_cov_4.109861, whole genome shotgun sequence.
ACCESSION   DAOSYN010000011 DAOSYN010000000
VERSION     DAOSYN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_16048_cov_4.109861"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..599)
                     /locus_tag="PLJ11_01045"
     CDS             complement(108..599)
                     /locus_tag="PLJ11_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03292.1"
                     /translation="MDFNSIDDISDHGFQGFVPIQKLMNPEGTLIVDNKKGVYFVLRI
                     SKEPPKFLEIGTGAIVPGKVTNDPISVLESKWVDGTIVMYIGMAKISLRKRLQKYMRF
                     GMGLHSNHHGGRHIWQLADSRELLVCWYVLPWDVDPETFEKAIISEFKEIYGKYPFAN
                     RRL"
     gene            complement(622..1203)
                     /locus_tag="PLJ11_01050"
     CDS             complement(622..1203)
                     /locus_tag="PLJ11_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03293.1"
                     /translation="MPGHAFHEKIGDIVTSIVNKDDERTYCLLSDDCSRKDREGGYHA
                     LKIFGNDEDGNSMEYARADLLIVHDGLIVGQVEIEEEDRSPTKLFGKIISSAHSTYYE
                     YKSGLAPKEKGPIKFSDRFKFIQIVRYDKSEYTKESGHPAKWERVERDIKSMLNNSEI
                     GFSSPTKRKIEYKLLFGDIREIEGTLGKIMLKF"
     gene            complement(1432..1518)
                     /locus_tag="PLJ11_01055"
     tRNA            complement(1432..1518)
                     /locus_tag="PLJ11_01055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1482..1484),aa:Ser,seq:gct)
     gene            2204..3671
                     /locus_tag="PLJ11_01060"
     rRNA            2204..3671
                     /locus_tag="PLJ11_01060"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
     gene            3861..5474
                     /gene="pyrG"
                     /locus_tag="PLJ11_01065"
     CDS             3861..5474
                     /gene="pyrG"
                     /locus_tag="PLJ11_01065"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HOO03294.1"
                     /translation="MKYVFVTGGVISGLGKGITASSIGRLLKSRGAKVTAIKIDPYLN
                     VDAGTMNPFEHGEVFVLDDGGEVDLDLGNYERFLDISLTSAHNITTGKVYKSVIEKER
                     MGEYLGKTVQIIPHVTNEIKAHIINVAEATGADVCIVELGGTVGDIESMPFLEAVRQM
                     NHDLGKGRNCLFVHTTLVPVMGTVGEQKTKPTQHSVKELRAIGIQPDIIVARCKDPLE
                     EPIRRKISLFCDVPVEAVVTAADARSIYDVPMALEAQGLTDYIMQRLGLAEKTRDLEG
                     WKDFARRINEQERTVRIACVGKYTNLADSYISHLEAFHHAGAEAGTRVQVTFVDSEVV
                     QQYGITEELLGADGVLIPGGFGVRGIEGKIATAKFARENRIPFLGVCLGFQIATIEFA
                     RNVLGMEGANSTEFDPDTPFPVVDLLPEQKNVKRLGATMRLGAQPVMVEEGTKAFELY
                     GKALIMERHRHRYEINPKHIEELETGGWRFTGRSADGVKMEIGEMRGHDFYLASQFHP
                     EFKSRPNRPSPLHLGLVKAAVARKYGATQ"
     gene            5513..6130
                     /locus_tag="PLJ11_01070"
     CDS             5513..6130
                     /locus_tag="PLJ11_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HOO03295.1"
                     /translation="MPNDDYLSLLDRAKTQLPETIEKHERFVVPEPDVFQEGKTTVFR
                     NFGDVVDALRREPEHVLQYLLRELGTPGTLEGRRVIFKTRLTSAQIADKVQSYVDTFV
                     VCSECGRPDTHITKEDRVLILECEACGAHRPVNVRKVQKPTEEGLREGGVYEMMIEDV
                     GKKGDGVARMFDYIIYVPGTVKGSRVKVKVAKITGKVAYGNVSME"
     gene            6185..7096
                     /locus_tag="PLJ11_01075"
     CDS             6185..7096
                     /locus_tag="PLJ11_01075"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF041679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HOO03296.1"
                     /translation="MRTARQYVLLIGMFLAVPLAALLVVPSVPSGYRAFQDQNDPTSP
                     IVFIILVVGMTGAMLVLIKRARRGLLKALLMGALGVGLFSIGSLFLLLAGQGEAFSLG
                     FGIALAAGVLALLYKYPRWYVVNAAGLIIGAGAALVLGITLGILPSLILLTVMAMYDA
                     ISVYVTKHMLTLAEGVGDLGLPIVLVVPGKHGTDLAQLDLKDRSKDAEREMMLMGLGD
                     AVIPSVLVVSAAANLSPVSGAGWPWPAMSVALLTLVAVTIGLAALMLLISKGRPQAGL
                     PFLNGSAIIGYALSYLLVYQDLGFGFA"
     gene            7134..7772
                     /locus_tag="PLJ11_01080"
     CDS             7134..7772
                     /locus_tag="PLJ11_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOO03297.1"
                     /translation="MSGRYLVLSDVHRREWVAEAARELMREHSLEEAVLLGDITHFGP
                     PAYAEDFIRSLGARCYAVPGNCDPLGTEAFIERSAVCLHRHKVRVDGRTWGGLGGSNP
                     SVFRTPFEMPEEEISRSLRPIMEEGMVLVLHCPAYGTFDLTYSNRHVGSRAVRDAVER
                     CKPAVVLSGHIHEDRGVTMKEGVWYMNPGAAKDHYAGMMELGDEVRLTLLDL"
     gene            7826..8209
                     /locus_tag="PLJ11_01085"
     CDS             7826..8209
                     /locus_tag="PLJ11_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HOO03298.1"
                     /translation="MAIWQGRSKRKPTGGRLTLSRKKRRYEIGKEPVFTLVGSEKRSV
                     SRVRGANTKVRLLKAQFANVVDPATNKTQKAKIITVKESPANPNYVQRNIITKGAVIQ
                     TELGPAKVTSRPGQDGSINAILVKE"
     gene            8226..8519
                     /locus_tag="PLJ11_01090"
     CDS             8226..8519
                     /locus_tag="PLJ11_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0008312 - 7S RNA binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HOO03299.1"
                     /translation="MALDEDTAWVLWPEYFDRSRPRSRGRKVKKGLAVVNPTVDMISA
                     ALRQLGLQHKVEREKRYPANWFSSGGRVLVERTGPKSQLVLKVGELLASAQRS"
     gene            complement(8507..8797)
                     /locus_tag="PLJ11_01095"
     CDS             complement(8507..8797)
                     /locus_tag="PLJ11_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF211 domain-containing protein"
                     /protein_id="HOO03300.1"
                     /translation="MSEIKRLVLDVLKPHHPSIIDLATRLSVVEGVSGVNCTLDEVDQ
                     ETESIKITIEGTAINYEDVEEVIIDSGAVIHSVDSVSAGKKLVEEVETPQDR"
     gene            8891..10321
                     /locus_tag="PLJ11_01100"
     CDS             8891..10321
                     /locus_tag="PLJ11_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOO03301.1"
                     /translation="MIEQEEVRVLDINSEFLGVPPEVLMENAGQVVAKEITSRFGEGL
                     RIAILCGTGNNGGDGFVTARALMKANRVRVLLALPPSHIRSPEARLNFEKVEELHGVY
                     SGEDLSAYDLLVDALLGVGAKGEPREPYATLIRAINASGRPVVSVDVPSGMGTGLAVR
                     PAVTVTFHDLKVGMDEGRCGEIVVKDIGIPAEAERYVGPGDLVHFPLPSPDSHKGQNG
                     RLLVVGGGPYTGAPTLAGLAAYRVGTDLVFVACPDVVRGVIASYSPNLITVPLAGRRF
                     GVGSVEGTLALCKGMDAVLLGPGLGDDEDTLAGVRGVLSKCTLPMVLDADALKAVAGH
                     LDMLKGKKGVLTPHHGELEGLIGQPVPKDDGGRTEAAKELAARTGWTVLLKGRVDVIT
                     DGQRVKLNRTGNAGMSVGGTGDVLAGITAGLLSKGMAPFNAARAAAYLNGSAGDLAFE
                     RYSYGLMATDLLERVPRVLQRCLSKL"
     gene            10335..11009
                     /locus_tag="PLJ11_01105"
     CDS             10335..11009
                     /locus_tag="PLJ11_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOO03302.1"
                     /translation="MKVGLISDVHANLPALEAVLQDMSARNVGTVYCAGDILGYYTFP
                     DEVVELLRGREVHCIAGNHDRAVLVGTKGMNSIAAAAIEWTRGHISSSTYAFIRDLPN
                     SIHRPVDGVMAAIHHGSPRHMSEYIFEEHVSDELLAVAGAKLLVLGHTHMPYIAQFPS
                     GHVINPGSVGQPRDGDPRASYAILDTAEWTFSMVRVGYDQRTVIAGVRENGLPEMLAS
                     RLLRGA"
     gene            11010..11786
                     /locus_tag="PLJ11_01110"
     CDS             11010..11786
                     /locus_tag="PLJ11_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOO03303.1"
                     /translation="MVSKAVPVPDHPALEVLGEERVLCVGDLHIGIETDMRFRGIVIP
                     SQTNRMQRELIDLAKGVDRLVIIGDVKHQVPGSTKQEHAEVPRFFNSLLGHYPRIDIV
                     KGNHDTDIERMVPAKVTVHDQGGLVLDDVGFVHGHAWPSQEVMASRLLIMGHNHPAVV
                     FEDGLHHNQVERCWVRCRFRSGGGRYERVPDEAIMVPAFNRSLGGGPVNLEGPRMLGP
                     LLANELVDLANGQVYLMDGLLLGSVSALRVKRRGYFRMED"
     gene            complement(11840..13321)
                     /locus_tag="PLJ11_01115"
     CDS             complement(11840..13321)
                     /locus_tag="PLJ11_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOO03304.1"
                     /translation="MTEKRITIDPITRLEGHGKIEIFLDDNGNVANAYWQVPELRGFE
                     KFCIGRSVDELNKLTSRLCGVCPGAHHICSTKALDGVFGVEPPETAKKLRELFYMAHY
                     IHSHIAHFYALAAADFVLGPGAPKAERNILGVVAAVGVEIGGEVIKHRSYAQKIQEML
                     GGKATHPVCGIPGGFSKSISEDERAKIEAMAKSCVEFSKFTNQLFADVVLKNKDYLAL
                     ITDPGIYYNETYYLGTVDKNDKINFYDGTQKMISPTGEEVARYTGKDYLKYIAEHTLP
                     WSYEKFCYFKPVGWKGLVDGKDSGIYRATPLARLNVSKGFTTPLAQAEYEKYFGTFRD
                     LGVKGPIHHTMATHWARVIEMLYASEKLLELSQDPEITGKHVRNPVKEPGEGVGILEA
                     PRGTLVHHYVADKNGITKDVNLVVGTTNNNAPMNLSVRKAATALIKNWQVSDGLLNAV
                     EMAYRAYDPCNSCATHTLPGQMPIDAVIRRADGSVYRTLSRSC"
     gene            complement(13371..14390)
                     /locus_tag="PLJ11_01120"
     CDS             complement(13371..14390)
                     /locus_tag="PLJ11_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOO03305.1"
                     /translation="MAKIRIAQYWAAGCGGCDVALLDIDEKILGVAEIADIAFWPIAV
                     DAKVKDVEAMPDGYITATLINGAIRNSENEHMAKLLRQKSALIVSFGSCACFGGVPGL
                     ANMTSKKEILEYVYWHTPTSDNVEKVLPTPHYKVPEGEIEIPVLYDTVYTLDQVIDVD
                     YYMPGCPPTTNLINEFLGMVEKHVKEGAPLPPKGSVIASQKTLCDECARKRTTKKVEK
                     IYMPYEIDIDPEKCMLEQGIICLGPSTRAGCGAKCLEGNQPCRGCMGPTAEVLDQGGS
                     MLSALASIFAIGNQESQLSEDDILELMTQVKDPLGTFYAFTMPASIIKKKVKEKKATT
                     APKEA"
     gene            complement(14404..14856)
                     /locus_tag="PLJ11_01125"
     CDS             complement(14404..14856)
                     /locus_tag="PLJ11_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOO03306.1"
                     /translation="MAEEAQAQSAPVEAEGWEPKLLVFCCNWCSYAGADLAGVSRLQM
                     PTNFRVIRTMCSARVDPEFVLRAFAKGADGVLVLGCHPADCHYIGGNYRTRRRIALLR
                     VLLEQYGFNPDRLKLEWVSASEGMKFQQTIKEFDRTIRELGPNPINEE"
     gene            complement(14856..>16048)
                     /locus_tag="PLJ11_01130"
     CDS             complement(14856..>16048)
                     /locus_tag="PLJ11_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO03307.1"
                     /translation="IAAQGFKVDILEATGAMGGNMVNLYTAEGGISPRQYIKDLEAKV
                     RANGKITVHLNTKVEDVSGFVGNFKVKVTGGKEIETGAIVIATGAKAYVPTEYNYGKD
                     KKVVTQNELEKALAEGKFAARNVVMVQCVGSRNDAAPYCSRVCCSKAVKNAIEIKKRD
                     PKAQVYILHKDIRTYGFRETLYKEASGLGVKFIRYPENKMPEMTADGKIVVEDTILGA
                     PVQLSPDCLVLSVGVRPNEDNEELAKMCKVPLSKDKYFLEAHMKLRPVDFATSGVYLA
                     GLAHWPKFIDETIAQASGAASRAMTIISKQYLETQGIIAAVNEMVCNGCGVCEPVCEY
                     RAITIVGDPANPDKRKAVINEGLCMGCGTCVAACPSGALEQKGFKNNQILAQIDAALV
                     GGGK"
BASE COUNT         3205 a         4543 c         4963 g         3337 t
ORIGIN      
        1 aactgtcaac atctaaaatc agtgaaattt cgcccggtca actgtcgaac tcaggattga
       61 tcggtttcaa taatagcaga ggggacactc ctccatattg gcaataatca taatctacgg
      121 ttggcaaatg gatattttcc ataaatttcc ttaaattcag atattatggc tttctcaaat
      181 gtctcaggat caacatccca tggtagaaca taccagcaga ccagcaattc cctgctgtcc
      241 gccagctgcc agatgtggcg cccaccatgg tgattagagt gcagccccat tccaaatctc
      301 atgtattttt gtaatctctt tcttaaggag attttcgcca tcccaatata catcacaatg
      361 gtaccatcaa cccatttaga ttcaagaacg gagattgggt cgtttgtcac ttttccaggg
      421 acaatcgctc cagttcctat ttctaaaaat ttggggggct ccttgctaat gcgcaggaca
      481 aaataaacac cctttttatt atcaactatt aaagtccctt ccggattcat caatttttga
      541 atgggaacga atccttggaa cccatgatca cttatgtcat cgattgaatt gaagtccatg
      601 tttcatccac gtcaatttta atcagaactt caacatgatt tttccgagag tcccctcgat
      661 ctctctaata tcgccgaata gtagtttgta ttcaattttt cgtttggtcg gactggaaaa
      721 tcctatttca ctgttattta gcatggattt tatgtctctt tccacccgct cccacttagc
      781 tggatggcca gattctttgg tatattcact cttatcataa cggacgattt gaatgaattt
      841 aaatcggtcg ctgaatttaa taggaccctt ctcctttgga gctaatcctg atttgtattc
      901 gtagtaggta ctatgagctg atgatattat tttaccgaag agtttggtgg gtgaacgatc
      961 ctcctcctca atctcaacct gtccaacaat aagaccgtca tgaacaatca acagatcggc
     1021 acgggcatac tccatgctat ttccatcctc atcatttccg aatatcttga gagcatgata
     1081 gcccccctcc ctatcctttc tggagcaatc atccgaaagc aggcaatagg tgcgttcgtc
     1141 atccttattg acgatggagg tgacaatgtc cccaattttt tcatgaaatg catgaccggg
     1201 catgatttat cataattcca tttgggattg aaaaatgttc gtgttacaat tcaatctttc
     1261 tatttgatac aaaacaaacc cctcacattc tctcgttttt aaattatcga ttcctcccca
     1321 acttcccaaa aagataaaga aaaaagaggt ttaggatggt cgatagccag atagcccagg
     1381 tacaaaaacc ctttaattga cgtaaatatt ggaataactg gataggataa atggcgcgaa
     1441 gggggggatt cgaacccccg ggtccttgcg gacaatggat tagcaatcca tcgccttacc
     1501 gggctgggcc acctccgctt ttcggctgca aaggcgagag tcgttattaa tagcttttgt
     1561 aatagagggt cgcaaaacta ggatgcctgg tcttcgaggg accgcatcgc tggcacgttg
     1621 acggggcatc agggccccgg acccttggcg ccccttcccc gtcagattga cccttgtctt
     1681 tagatctgat agacccctag agtatcgccc cacggaaagg aaggtcggac ggaccgatgg
     1741 tgtccccggc ctctattaag gaaggtttga cggtgcacta tatgcaccat gtatagggta
     1801 tcgcggagag gggatatgcc gaattgggac cgctcctatg gaaaaggtga aataggccgg
     1861 gaagaatgaa taatataaga gcgcggaact gttcgttcga ggcggtctga tgcctctgaa
     1921 aggacaatcc ccgaaacggt tatataatag accgaagtta aggggattct gcgctgaaac
     1981 aacgcgccaa accgccgaac tggatgcttg catcgaggga ggtgggaagg aatgggaatg
     2041 gagaatcaag atctccgtcg aaaggcgtcg acgtggcctc ttggttctga cgctcgatgg
     2101 cagtaacgct tctttggaag tggtactgct ggtgaaactt ggtaactctg ccaatttctt
     2161 caataacatc ctaggcgatc aattaacggt tgtcgatgga tcaattccgg ttgatcctgc
     2221 cggcggccac cgctattgga attcgattaa gacatgcgag tcgagagtcg caaggactcg
     2281 gcggaccgct cagtaacacg cggataacat gcccttgggt gggggataac ctcgggaaac
     2341 tgagaataat accccataga cctatatggc tggaatgcct ttaggccgaa aactccggtg
     2401 cccaaggatt ggtctgcggc gtatcaggtt gtagtgggtg taacggaccc cctagcctat
     2461 gacgcgtaag ggccttgaga gagggagccc tgagatggac tctgagacat gagtccaggc
     2521 cctacggggc gcagcagtcg cgaaaacttc acaatggggg aaaccccgat gagggaattc
     2581 caagtgccag cacattgtgt tggctgttct ctggtctaaa aaaccggaga agtaagggcc
     2641 gggtaagacg ggtgccagcc gccgcggtaa tacccgcggc ccgagtggtg gtcgatatta
     2701 ttgagcctaa aacgtccgta gccggtcttg taaatccttg ggtaaatcga ccagcttaac
     2761 tgttcgaagt ccggggagac tgcaagactt gggatcggga gaggtgagag gtacttctgg
     2821 ggtaggggta aaatcctgta atcctagaag gaccaccggt ggcgaaggcg tctcactaga
     2881 acgaatccga cggtgaggga cgaagccctg gggcgcaaac gggattagat accccggtag
     2941 tccagggtgt aaacgctgcg ggcttggtgt tgggggtcct ttgagggcgc ccagtgccgg
     3001 agagaagttg ttaagcctgc tgcttgggga gtacgtccgc aaggatgaaa cttaaaggaa
     3061 ttggcggggg agcaccgcaa cgggaggagc gtgcggttca attggattca acgccggaca
     3121 actcaccagg agcgacggtt acatgaaggc caggctgatg accttgcctg attttccgag
     3181 aggtggtgca tggccgtcgt cagttcgtac cgtaaggcgt tctcttaagt gagataacga
     3241 acgagaccct cgccgctaat tgccaatgct ctctccggag ggcacgcaca ttaacgggac
     3301 cgctgtcgct aagacagagg aaggagaggt caacggtagg tccgtatgcc ccgaatctcc
     3361 tgggctacac gcgcgctaca aaggttggga caatgggctc cgacatcgaa aggtgaaggc
     3421 aatctcgaaa cccaatcgta gttcggattg agggttgtaa ctcaccctca tgaagctgga
     3481 ttccgtagta atcgcttgtc aacatcaagc ggtgaatatg cccctgctcc ttgcacacac
     3541 cgcccgtcaa accacccgag ttgggtttca gtaaggatat ctcattttgg ggtcttcgaa
     3601 ctgagattca gcaaggaggg ttaagtcgta acaaggtatc tgtaggggaa cctgcagatg
     3661 gatcacctcc tacgcaaggg gaaacaccga aggcatttcg tgtgtttccc ccaccaattc
     3721 cggcagggtt accaggcacc aaaaacaaca tcgagcgtcc ccattcaatg catttttttc
     3781 tcaactccaa aatccctacc caggggcctg agcagaagtc ataaatatcc tcccagttta
     3841 tcgaaggctc ccgtgagggc atgaagtacg tttttgtcac gggaggggtc atatccgggc
     3901 tggggaaggg gataacagcg tcctcgatcg gccgcctgct caagtcccga ggggccaagg
     3961 taacggctat caagatcgat ccctacctca atgtggacgc agggacgatg aacccgttcg
     4021 agcacgggga ggtgttcgtc ctcgatgacg gcggggaggt ggacctggac ctgggcaact
     4081 acgaaaggtt cctggacatc agcctgacca gcgcgcacaa catcacgacc ggcaaggtct
     4141 acaagtcggt gatcgagaag gagaggatgg gggagtacct ggggaagacc gtacagatca
     4201 ttccgcacgt cacgaacgag atcaaggcgc acatcatcaa cgtggccgag gccacgggag
     4261 cggacgtgtg catcgtcgaa ctggggggca ccgtgggcga catcgagtcc atgcccttcc
     4321 tggaggcggt ccggcagatg aaccatgacc tcgggaaggg gcgcaactgc ctcttcgtgc
     4381 acaccaccct ggtcccggtc atgggaaccg tgggggagca gaagaccaag cccacccagc
     4441 actcggtcaa ggaactcagg gccatcggca tccagcctga tatcatagtg gcgaggtgca
     4501 aggaccccct ggaggagccg ataaggcgca agatatccct cttctgcgac gtccccgtcg
     4561 aggcggtggt aaccgcggcg gacgcccgct ccatttacga cgtcccaatg gccctggagg
     4621 cgcagggact tacggactac atcatgcagc gcttagggct tgcggaaaag accagggacc
     4681 tggaggggtg gaaggacttt gcgcggagga tcaacgagca ggagcgcacg gtccgcatcg
     4741 cctgcgtggg caagtacacc aacctcgccg attcctacat atcgcatttg gaggcgttcc
     4801 accatgccgg cgcggaggcg gggacgaggg tgcaggtgac gttcgtggac tcggaggtcg
     4861 tccaacagta tggtataacc gaggagctcc tgggggcgga tggggtcctg atcccggggg
     4921 gcttcggggt aagggggata gagggaaaga tagcgaccgc caagttcgcc agggagaacc
     4981 gcattccgtt ccttggggtc tgcctcggtt tccagatagc caccattgag ttcgcccgga
     5041 acgttctggg gatggagggg gcgaacagca cggagttcga cccagacacc cccttccccg
     5101 tggtggacct gctcccggag cagaagaacg tgaaacggct gggggcgacc atgaggcttg
     5161 gggcccagcc ggtgatggtg gaggagggga cgaaggcgtt cgagctctac ggcaaggcgc
     5221 tgatcatgga gcgccacagg caccgctacg agatcaaccc caagcatata gaggagctgg
     5281 agacgggggg atggaggttc accgggcggt ccgcggacgg cgttaagatg gagatcgggg
     5341 agatgcgggg gcacgacttc tacctcgcct cgcagttcca tcccgagttc aagtcccggc
     5401 ccaataggcc gtccccattg cacctgggac tggtcaaggc ggcggtggcc cgcaagtacg
     5461 gggcaaccca ataagcttta tatcgcatgg gggacttgga ggaccgatcg acatgcccaa
     5521 cgatgactac ctttccttgc tggaccgggc caagacgcag ctgccggaga ccattgagaa
     5581 gcacgaacgg ttcgtggtcc ccgagccgga cgtgttccag gaggggaaga ccaccgtctt
     5641 ccgtaacttc ggggatgttg tggacgcatt gcgccgggag cctgagcatg tgctgcagta
     5701 cctcctcagg gagcttggca cacccggcac cctggagggg cggcgcgtca tcttcaagac
     5761 ccgcctcacc tcggctcaga tcgcggacaa ggtgcagagc tacgtggaca cgttcgtggt
     5821 ctgctcggag tgcggaaggc cggacacgca catcaccaag gaggaccgtg tgctcatcct
     5881 tgaatgcgag gcctgcggcg cccatcgtcc cgtcaacgtc cgcaaggtcc agaagcccac
     5941 cgaggagggg ctgcgggagg gcggggtgta cgagatgatg atcgaggacg tgggcaagaa
     6001 gggcgatgga gtggcccgca tgttcgacta catcatctat gttcccggca ccgtgaaggg
     6061 gtccagggtg aaggtcaagg tggccaagat caccggcaag gtggcctatg gcaacgtatc
     6121 catggaatga ccttccgttc gcggaggaca ttaacgattt aagcctgtcc ggccatttcc
     6181 acccatgcgc accgccaggc agtacgttct tctgataggg atgttcctcg ccgtgccctt
     6241 ggcggcgctg ctggtggtgc cctctgtgcc cagcggctat agggcattcc aggaccagaa
     6301 cgacccgacg agccccatcg tattcataat actggtggtg ggcatgacgg gggcgatgct
     6361 ggtgctgatc aagcgcgccc gccgggggct gctcaaggcc ctgctgatgg gggcgttggg
     6421 ggtcgggctg ttctccatcg gttcgctctt cctgttgctg gccgggcagg gggaggcctt
     6481 ttcgctcggc ttcgggatcg ccctggccgc cggggtgctc gctctcctgt acaaataccc
     6541 tcgctggtac gtggtgaacg ccgcgggcct catcatcggc gcgggggctg cgttggtcct
     6601 cggcatcacc ctcggcatcc tgccgtccct catattgctc accgtcatgg ccatgtatga
     6661 tgccatctcc gtctacgtca ccaagcacat gctcaccctg gccgaggggg tgggggacct
     6721 gggcctcccg attgtccttg tagtgccggg caaacatggg accgacctcg cccagctgga
     6781 cctaaaggac cgaagcaagg acgccgagag ggagatgatg ctcatggggc tgggggacgc
     6841 ggtcatcccc tccgtgctcg tggtctcggc ggcggccaac ctttccccgg tgagcggggc
     6901 aggctggccg tggccggcca tgagcgtggc cctgctcacg ttggtcgcgg tgacgatagg
     6961 gctcgcagcc ctgatgctcc tcatctccaa gggcaggccc caggccggcc tgcctttcct
     7021 gaacggctcc gccataatag ggtatgccct ctcctacctg ttggtctacc aggacctcgg
     7081 cttcggcttt gcctgagcaa aatgctttat ccctttcgcc attaccgctg gatgtgagcg
     7141 gaaggtacct tgttctctcg gatgtgcatc gccgggagtg ggtggccgag gcggccaggg
     7201 agctgatgcg ggagcattcc ttggaagagg cggtgctgct cggggacatc acgcacttcg
     7261 ggcctccggc ctatgcggag gacttcatcc gttccctcgg cgcaaggtgc tacgccgtcc
     7321 cgggcaactg cgatcccttg ggaacggagg ccttcatcga gcggagcgca gtctgccttc
     7381 acaggcataa ggtgagggta gacggtcgga cgtggggagg attgggaggt tcgaacccga
     7441 gcgtgttccg taccccgttc gagatgccgg aggaggagat atcaagatcg ctgaggccga
     7501 tcatggagga gggcatggtc ctggtgctgc attgccccgc ctatggtacc ttcgacctca
     7561 cctactcgaa ccggcacgtt gggagcaggg cggtgcgcga tgccgtagag agatgcaagc
     7621 ccgcggtggt cctctccggg catatccatg aggaccgcgg ggtgaccatg aaggagggcg
     7681 tgtggtatat gaacccgggg gccgccaagg accactatgc agggatgatg gagcttgggg
     7741 acgaggtccg cctgacccta ctggacctct agaccagagg ccaaggtttt tatagacaag
     7801 cctatttagg tggcccagga ttgctatggc aatatggcaa ggtaggtcca agaggaagcc
     7861 cactggcggg cgtttgacgc tgtccaggaa gaaacggagg tacgagatcg gcaaggagcc
     7921 cgtcttcacc cttgtgggca gcgagaagcg gtcggtgtcc agggtgcgcg gggcgaacac
     7981 caaggtgcgc ctgctgaagg cccagttcgc caatgtcgtc gacccggcca ccaacaagac
     8041 ccagaaggcc aagatcatca ccgtcaagga gagccccgcc aacccgaact acgttcagcg
     8101 caacataatc accaagggcg cggtcatcca gaccgagctc ggcccggcaa aggtcacctc
     8161 cagaccggga caggacggct ccatcaacgc catcctcgtg aaggagtagg ggcacggggt
     8221 cgcccatggc tctggacgag gacaccgcgt gggtgctctg gccggagtac ttcgaccggt
     8281 ccaggccccg gtcccgaggg cgtaaggtca aaaaggggct ggccgttgtc aatcctaccg
     8341 tcgatatgat ctcagcggcc ctcaggcaac tggggctgca gcataaggtc gagcgggaga
     8401 agcgctatcc cgccaactgg ttctccagcg ggggaagggt gctggtcgag cgaaccgggc
     8461 ccaagtccca attggtcctg aaggtggggg agctgctggc aagcgctcag cggtcctgag
     8521 gggtctccac ctcttccacc agcttcttgc ccgcggacac gctgtccacg gaatggatga
     8581 ccgccccgga gtcgatgatg acctcctcca catcctcgta gttgatggcg gtcccctcga
     8641 tcgttatctt gatgctctcg gtctcctggt ccacttcatc taaagtgcag ttgaccccgg
     8701 acaccccctc gacgacgctg agcctcgttg ccaggtcgat tatggacggg tggtggggct
     8761 tgagcacgtc cagcaccaaa cgttttatct cggacatctt ggattcttcc agctttaaaa
     8821 tggggtcctg atataaattg cttgtcgatg tctggaaagg attattccca tccaaggcca
     8881 tgccctatcc atgatcgagc aggaggaggt ccgggtcctg gatatcaatt cggagttcct
     8941 gggggtgccg cccgaggtcc tcatggagaa cgccggtcag gtcgtggcca aggagatcac
     9001 ctcccgcttt ggtgaagggc tgcgcatcgc aatcctctgc ggtacaggca acaacggggg
     9061 ggatggcttc gtgaccgcca gggcattgat gaaggccaac cgcgtaaggg tgctactggc
     9121 attgccccct tcgcacatac ggtcccctga ggcccgcctc aacttcgaga aggtcgagga
     9181 actgcacggg gtctactcgg gggaggacct ctccgcctat gatctgttag tggacgcgct
     9241 cctcggcgtc ggggccaagg gcgagcccag ggagccgtat gcgacgctca tcagggccat
     9301 caacgcctcg ggcaggccgg tggtctcggt cgacgttccc tctgggatgg ggaccgggct
     9361 ggccgtccgt cccgcggtca cggtgacctt ccatgacctc aaggtcggaa tggacgaggg
     9421 acggtgcggg gagatcgtgg tgaaggatat aggcatcccg gccgaggcgg aaaggtacgt
     9481 cgggccgggg gacctggtac acttcccgct gccctccccg gactcgcata agggacagaa
     9541 tggaaggttg ctggtggtcg gtgggggccc ctacacgggg gcgccgaccc tcgcggggct
     9601 ggccgcatac cgcgtcggga cggacctggt cttcgtggcc tgccccgacg tggtccgggg
     9661 ggtcattgcg agctattccc ccaacctgat cacggtccct ttggctggga ggagatttgg
     9721 cgtcgggagc gtcgagggga cgctcgccct ctgcaaggga atggacgcgg tcctcctggg
     9781 cccggggctc ggggatgacg aagacacctt ggctggagtg agaggagtct tatcgaaatg
     9841 caccttgcca atggtgctcg acgcggatgc attgaaggcc gtggccggac atctggacat
     9901 gctcaagggc aagaaagggg tcctcacccc gcaccacggg gagctggagg ggctgatcgg
     9961 ccagcccgtg cccaaggacg acgggggaag gaccgaggcg gcgaaggaac tggccgccag
    10021 gaccggctgg accgtcctcc tcaaagggag ggtggatgtc atcaccgacg ggcagagggt
    10081 caagctcaac cggaccggca acgcgggcat gagcgtgggc ggcacggggg acgtgctggc
    10141 ggggatcacc gccggcctgc tctcaaaggg gatggccccc ttcaacgccg cccgcgccgc
    10201 cgcctatctc aatggttcgg cgggggacct tgccttcgag agatacagct atggcctgat
    10261 ggccacggac ctgctcgagc gtgtcccgcg ggtcctccag cgatgcctgt cgaaactatg
    10321 aaggaggtat gcagatgaag gtcggcctga tctcggacgt ccatgcgaac cttccagcct
    10381 tggaagcggt cctgcaggac atgagcgctc ggaacgtggg aacggtgtac tgcgcggggg
    10441 acatccttgg atactacacc ttcccggatg aggtcgtgga gctcctgagg gggagggagg
    10501 tgcactgcat cgccgggaac cacgacaggg ccgtattggt ggggacaaag ggcatgaaca
    10561 gcatcgccgc cgctgccatt gagtggacga gggggcatat ctcctcatcc acgtacgcct
    10621 tcattcgcga tctgccgaat tccatccatc ggccggtgga tggggttatg gcggccattc
    10681 accacgggtc cccacggcat atgtccgagt acatattcga agagcacgtg agcgacgagc
    10741 tgctggcggt ggccggcgcc aagctcctgg tgcttggcca tacccatatg ccgtacatag
    10801 cgcagttccc gagcggccat gtcatcaacc ctggttcagt ggggcagccc agggacggtg
    10861 accctcgggc ctcctacgcc atccttgata cggcggaatg gaccttcagc atggtgcggg
    10921 tgggctacga ccagcgcacc gtgatcgccg gggtgaggga gaacgggttg ccggagatgc
    10981 tcgcctcccg gctgctcagg ggcgcgtaga tggtgtccaa ggcggtcccg gtcccggacc
    11041 acccggcgct tgaggtcctg ggtgaggagc gcgtgctgtg cgtcggggac ctgcacatcg
    11101 ggatagagac ggacatgcgc ttccggggga tagtcatccc gtcgcagacc aatcgcatgc
    11161 agcgggagct cattgacctg gcgaaggggg tagaccgtct tgtgataatc ggggacgtca
    11221 agcaccaggt ccccggttcg accaaacagg agcatgcaga ggtcccccgg ttcttcaata
    11281 gcctgttggg ccactacccg cgcatagaca tcgtcaaggg caaccacgac acggacatcg
    11341 agcgcatggt gcccgccaag gttaccgttc atgatcaagg aggccttgtc cttgacgatg
    11401 tagggttcgt gcacgggcat gcatggccgt cccaggaggt catggcgtcc cgccttctga
    11461 taatggggca taatcatcct gcggtcgtct tcgaggacgg gctccatcac aaccaggtgg
    11521 agagatgctg ggtgcgctgc cggttcagga gcggaggggg aagatacgaa agggtcccgg
    11581 acgaggcgat catggtcccg gccttcaacc ggtccttggg gggaggaccg gtcaatctgg
    11641 aagggccgag aatgctcggc cccctcctgg ccaatgagct ggtggacctg gcgaacggcc
    11701 aggtgtattt gatggacgga ttgctcctcg gcagcgtatc ggcgctcagg gtcaaacgga
    11761 gagggtactt tcggatggag gattgaccct taaggcctcc gcgcaaaata aaaaacagga
    11821 aaaggttggg aaaggggttt cagcagctgc gggaaagggt cctgtagacc gagccgtcgg
    11881 cccgcctgat gaccgcgtcg atgggcatct ggcctggcag ggtgtgtgtg gcgcagctgt
    11941 tgcaggggtc gtaggccctg taggccatct ccacggcgtt gagcagcccg tcggacacct
    12001 gccagttctt gatcagtgcg gtggcggcct tgcggacgct gaggttcatg ggggcgttgt
    12061 tgttcgtggt ccccaccacc aggttcacgt cctttgttat gccgttcttg tccgctacgt
    12121 agtggtgcac gagcgtgcca cgcggggcct ccaatatacc gacgccctcg ccaggctcct
    12181 tcacagggtt gcgcacatgt ttgccggtga tctccggatc ctgggacagc tccagcaact
    12241 tttccgaggc gtacagcatc tcgatcacac gggcccagtg ggtcgccatg gtatgatgga
    12301 tcggcccctt gaccccgagg tcccggaacg tgccgaagta cttttcgtac tccgcctgcg
    12361 cgagcggggt ggtgaagccc ttcgaaacgt tcagcctggc gagcggggtg gcgcggtaga
    12421 tgccgctgtc cttcccgtcc acaaggccct tccagccgac cggcttgaag tagcagaact
    12481 tctcgtagga ccagggcagg gtgtgctcgg cgatgtactt caggtagtcc ttgcccgtgt
    12541 agcgggcgac ctcctccccg gtgggggaga tcatcttctg cgttccgtcg tagaagttga
    12601 tcttgtcgtt cttgtccacc gtgcccaggt agtaggtctc gttgtagtag atgccggggt
    12661 cggtgatcag cgccagatag tccttgttct tgagcacgac atctgcgaac agctggttcg
    12721 tgaacttgct gaactccacg caggacttgg ccattgcctc gatcttcgcc ctctcgtcct
    12781 cgctgatgct cttggagaac ccgccgggaa tgccgcacac cgggtgcgtg gcctttccgc
    12841 cgagcatctc ctggatcttc tgggcgtagg accggtgctt gatgacctcg ccgccgatct
    12901 ccactccgac cgccgcgacc accccgagga tgttccgctc ggccttcggg gccccggggc
    12961 ctaggacgaa gtcagcggcc gccaaggcgt agaagtgggc gatgtggctg tggatgtagt
    13021 gcgccatgta gaagagctcg cggagcttct tggccgtctc agggggctcg acgccgaaca
    13081 caccgtccaa cgccttggtg gagcatatgt ggtgcgcccc ggggcatacc ccgcagagcc
    13141 tggaggtcag tttgttgagc tcatccacgg acctgccgat gcagaacttt tcgaatcctc
    13201 tcagctcggg cacctgccaa taggcgtttg ccacgttccc gttgtcgtcc aggaagatct
    13261 cgatcttgcc atggccctcc aaccgggtga tcgggtcgat cgtgatccgc ttctcggtca
    13321 ccttctcaat cttatcgggt ccctgaccca tcaatatcgg gcacattcgt tcaggcctcc
    13381 ttgggggctg tggtagcttt cttttcctta accttcttct tgatgataga ggcgggcatt
    13441 gtgaacgcgt agaacgtgcc gagcggatcc ttgacctggg tcatcagctc caagatatcg
    13501 tcctcgctca gctggctttc ctggttgcca atggcgaata tcgaggccag cgcgctcagc
    13561 atgcttccgc cctggtccag gacctcggct gtggggccca tgcatccgcg gcagggctga
    13621 ttgccctcca ggcacttggc gccgcagccc gccctcgtgg acggccccag gcatatgatg
    13681 ccctgctcca gcatgcactt ctcgggatcg atgtcgatct cgtagggcat gtatatcttc
    13741 tcgaccttct tggtagtcct cttcctggcg cactcatcac agagggtctt ctgggaggcg
    13801 atgacggagc ccttgggggg caggggggcg ccttccttca cgtgcttctc gaccatgccc
    13861 aggaactcgt ttatgaggtt ggtcgtcggc gggcaaccgg gcatgtagta atccacgtct
    13921 atgacctggt ccagggtgta tacggtgtcg tacaggaccg ggatctcgat ctccccctcg
    13981 gggaccttgt aatgcggggt gggcagaacc ttctccacgt tgtcggaggt aggggtgtgc
    14041 caatacacgt actccaggat ctctttcttg ctggtcatgt tggccaggcc gggcaccccg
    14101 ccgaagcagg cgcaggaacc gaaggagacg atgagggccg acttctgcct cagcagcttg
    14161 gccatatgct cgttctcgct gttcctgatc gctccgttga ttagggtggc ggttatgtac
    14221 ccatccggca tggcctcgac gtccttcacc ttggcgtcca cggcgatggg ccagaacgcg
    14281 atgtcggcga tctcggcgac gcccaggatc ttttcgtcga tgtccagcag ggcgacgtcg
    14341 catcctccgc acccggcagc ccagtactgt gcgatcctta tctttgccat ttatctctcc
    14401 tccttactcc tcgtttatgg ggttcggccc cagttccctg atggtgcggt caaactcctt
    14461 tatggtctgc tggaacttca tcccctctga ggcggagacc cattccagct tcaatcggtc
    14521 tgggttgaac ccgtactgct ccagcagcac gcggagcagg gcgatcctcc ttctggtgcg
    14581 gtagttcccg ccaatgtagt ggcagtcggc ggggtgacag ccgaggacga ggacgccatc
    14641 ggcccccttt gcgaaggccc tcaggacgaa ctcggggtcc accctggccg agcacattgt
    14701 gcggatgaca cggaagttgg tcggcatctg cagacgggac actccggcca gatcagcccc
    14761 agcataggag caccagttgc agcagaacac caacagcttc ggctcccagc cctcggcctc
    14821 cacgggggcg ctctgggcct gcgcttcctc ggccatcact taccacctcc gacgagggcg
    14881 gcgtcgatct gtgccaggat ctggttgttc ttgaagccct tctgttcgag ggcgccggac
    14941 gggcacgcgg ccacgcaggt cccgcatccc atgcacaggc cctcattgat cacggccttg
    15001 cgcttgtccg ggttcgccgg gtcgcccacg atggttatgg cgcggtactc gcacacgggt
    15061 tcgcacacac cgcaaccatt gcataccatc tcgttgaccg cggcgatgat gccctgggtc
    15121 tcgaggtact gcttcgagat gatggtcatg gcccttgagg cggccccgga ggcctgagcg
    15181 atggtctcgt cgatgaactt cggccaatgg gcaagccctg ccaggtatac gccgctggtg
    15241 gcgaagtcga ccggcctcag cttcatgtgc gcctcgagga agtacttgtc cttgctcaag
    15301 ggcaccttgc acatcttggc gagctcctca ttgtcctcgt tcgggcggac cccgacgctc
    15361 aggacaagac agtccgggga aagctggacc ggggcgccga ggatggtgtc ctcgaccacg
    15421 atcttgccgt ccgcggtcat ctcgggcatc ttgttctccg gataacggat gaacttgacg
    15481 cccagcccac tagcctcctt gtacagggtc tccctgaagc cataggtcct gatgtccttg
    15541 tgcaggatgt agacctgggc cttggggtcc ctcttcttga tctcaatggc gttcttgacc
    15601 gccttggagc agcatacacg gctgcagtac ggggcggcat cgttcctaga gccaacgcac
    15661 tggaccatga cgacgttcct ggcggcgaac ttgccctccg ccaaggcctt ctccagctcg
    15721 ttctgggtga ccaccttctt gtccttgccg tagttgtact ccgtcggcac ataggccttg
    15781 gcccctgtgg caatcacgat ggcaccggtc tcgatctcct tgccgccggt gaccttcacc
    15841 ttgaaattgc ccacgaatcc ggagacgtcc tccaccttgg tgttcaggtg aacagttatc
    15901 ttaccgttcg cccgcacctt ggcctccagg tccttgatgt actgcctggg ggagatccct
    15961 ccctcagcgg tgtacaggtt gaccatgttc ccgcccatgg cacctgtggc ctccaagatg
    16021 tcgaccttga agccctgggc ggcgatgt
//
LOCUS       DAOSYN010000012        15770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_3107_length_15770_cov_4.438562, whole genome shotgun sequence.
ACCESSION   DAOSYN010000012 DAOSYN010000000
VERSION     DAOSYN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 15770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15770
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3107_length_15770_cov_4.438562"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PLJ11_01135"
     CDS             <1..689
                     /locus_tag="PLJ11_01135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO03308.1"
                     /translation="TREIVRGVEMDLDVRAEDQRKVRVFLGKAVAPGFFAWAIPCGAM
                     IRVGLCVSEGNGPPSAYLRPLLATLGLSEAERVRAYSGAIPLGHIARTYADRALVAGD
                     AAGMAKPISGGGLFTGMTAGRLAAETFLEAFRGNDLTARTLSSYEGRWKAAFGSELRN
                     SYLVRRAFVRMSDRELDRVGAQLDTERAKEVLATGDIDFPTALAPGLLRACPSLMALA
                     PGLLLRLLRR"
     gene            693..1697
                     /locus_tag="PLJ11_01140"
     CDS             693..1697
                     /locus_tag="PLJ11_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HOO03309.1"
                     /translation="MRALKVPKREADAVKDRLNRLGQLDLGRRPGRQGGSVLLPVTGE
                     GPFPPYPLVDVELEPQDRHRAPQERIAERLDLPGELKELLPNKYERLGHVLVLRLPGP
                     LLPYRHAIAEAYAHVLKARTVLLERGTIHGVERRPDMELLYGTETETVHLEAGIRYAL
                     DPARVMFSSGNFDEKRRMASLDCRGETVVDMFAGIGYFTLPLAVQARADRVIACEINP
                     EAVRFLRRNLRLNGVGDRVTVFEGDNRDLPGEGFADRVVMGYVNVTWQFLPKAFSLVR
                     RGGILHYHDTCSIDRIPEGLLARVREGSGGRPYEVLGIREVKAYAPSISHMVVDIRAL
                     "
     gene            complement(1684..3186)
                     /locus_tag="PLJ11_01145"
     CDS             complement(1684..3186)
                     /locus_tag="PLJ11_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03310.1"
                     /translation="MPPRPRFRGIKKTPKNMEKEMLATSQRLADDPSLVIPVLLEEGR
                     RSPFAHIEKGIAKVLRYRDDPERLIKLASKGDQFVRAYAAAISLAASGKLPYLTTANL
                     PIGPVSFAIRGTVDKEKLIGLQHFDDPDLRLLAYWDMAREARLHIYSTEGGLFASVSG
                     PKAPEAYVKEVLANLPYRLEGSGCGHGDRPAVRIQWLSANVEIRVCAECGAGVNTAQH
                     LMARVAAPDPYDDLRVSVDHRYGGARPECQGEFPTPASLVREYLEGDLDDAGLITAHI
                     KAKGEWMRSRGQVYVLGQECYGKDGEAFLGALKGTDLERTALRAVLSLGIPIVTEQNQ
                     AGKVIGELWEAHARTMLTAVSDEGIAQRVLGMRELTPGQMLSEADRLVREREVLGNLP
                     AYSGLGPVGQLADSLAKAYRIEGTAAMLRLVERTEKEHTHRAVCYAFLTAVGEAQSRG
                     WQFNKEERESGEHLARHASRMLSSTGEGYHEALSALLAESGSGEVALRAP"
     gene            3258..3977
                     /locus_tag="PLJ11_01150"
     CDS             3258..3977
                     /locus_tag="PLJ11_01150"
                     /inference="COORDINATES: protein motif:HMM:NF017570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF835 domain-containing protein"
                     /protein_id="HOO03311.1"
                     /translation="MARDGLGREYADGYLKGYEDGLKEALEQLIATTCRKNYSSTEIQ
                     LLVKNQRASVPKKMALRRRQLERELGLDLERKEEGPEPRREAAPGRTVLLKGEGNQEA
                     LEAFRDLVQAGWDGLCISRMSPRDVRQFVGKDCTVLWLTKADPMAGDQDREEYLQPTE
                     TAKMQTSIRNFLSKNKGRPSVILLDGMTFIVTNTDFRSFLKIAQKLKDDVYQARSLLL
                     LPYEPKNLLENEVNLFINEMS"
     gene            complement(3974..4246)
                     /locus_tag="PLJ11_01155"
     CDS             complement(3974..4246)
                     /locus_tag="PLJ11_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014029.2"
                     /note="Sso10b; forms dimers; interacts with silencing
                     protein Sir2 which regulates Alba by deacetylation of a
                     lysine residue which affects DNA binding affinity; binds
                     double-stranded DNA tightly distributed uniformly and
                     abundantly on the chromosome; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOO03312.1"
                     /translation="MGESVIYIGQKPVMNYVLAAGKKLAEPGASILVQARGKCISKAV
                     DVALITLDRSKDSVKLGGISISTEVLPGPDGAPQNISSIKIALNRP"
     gene            4504..5049
                     /locus_tag="PLJ11_01160"
     CDS             4504..5049
                     /locus_tag="PLJ11_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03313.1"
                     /translation="MQENELSLTSEDMQDMAKEAIIIWWSTVMKAVVMSSNTPEMRRI
                     LHSFLAEAVTEMVKVNGSQRTLELMGPIMRQGGKKAAVRALEEHEIVDRDGIAISSLI
                     DLWEGALGVRGEIVERSETSVVKVAKQCPFANSPPELCSLFGRFVDGFCEVINPQYGW
                     FQTHRMSAGDEECRWVCRKEG"
     gene            5058..6773
                     /gene="polX"
                     /locus_tag="PLJ11_01165"
     CDS             5058..6773
                     /gene="polX"
                     /locus_tag="PLJ11_01165"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOO03314.1"
                     /translation="MENNAKVASILEEVADLLELKEDTFFKVRAYRRAAKEIAALTED
                     IKDIYIRGHLDKVPGVGRAIHDKVVEILRTGQLQYLNDLRNEFPAGLLQVMQVPDIGP
                     KTAARLYKELRITNLQDLKEAAEQHRIRMLKGFGEKTEENILKGIRYLESRQGRMLLG
                     YAFPRGRALEDHMRKQGFALVSLGGSLRRMKETIGDIDILVGSSEGERAMDAFVSHPN
                     VAEVLLRGETKTSIRLQDGVQVDMRVVAPSSFGAALQYFTGSKEHNVNMRSLASELGL
                     KVNEYGVFRLVDGARVAGETEEEVYAALGMQWMPPEMRENRGEVELARKGQVPKVVGL
                     PDIRGDLHIHSEASDGVDGVGALAAAAEARGYDYLAITDHSQSLTVGNGLSVERMLEN
                     IGHIRTVQEEHPGVHLLAGAEVEIDAKGGLDYPQGLLEELDVVVAAVHSHFKMAPKEM
                     TERLLSALSNETVDVLAHPTGRIIGEREGYGFDMERVMRAAKDNGVALEVNSFPERLD
                     LNDVQCAIARERGVTVCINTDAHNVRQLDNMVYGVATARRGWVPPGMVLNALPWTALK
                     DRLDL"
     gene            6821..7747
                     /locus_tag="PLJ11_01170"
     CDS             6821..7747
                     /locus_tag="PLJ11_01170"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HOO03315.1"
                     /translation="MSAEDVAERIRSMEIRGAAEIARRAALALREEAEAYEGNDLEGL
                     RSHLERAKELLLASRPTAISLWNGVQYVFKDTSKATTAEEYRSKVRENAEHFVKRSQS
                     ALRTIGRLGANRVREGDRVLTHCNSKAAIAVMAEAWAQGKRFEVYATESRPWRQGLLT
                     ANDLARLGIPVTMIVDSAVRYVMKDMDLVLVGADTIASNGALINKIGTAQVALAAHEA
                     RVSFTVCAETFKFSPRTIYGELVKIEERDPTEVVKEGEVPPQVKVFNPVFDATPPEYI
                     DSIVTEIGVIPPYAAYQVIIKELGQEFIFHEE"
     gene            7752..8996
                     /locus_tag="PLJ11_01175"
     CDS             7752..8996
                     /locus_tag="PLJ11_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HOO03316.1"
                     /translation="MEYEERYLHLNEPIDADAHVICKYKVGTDLKMTTAAAAIATEQS
                     TGTWTKLSTLREENFQRRSGKVTAIEGNVATIAYPVEDFSLDIGGVPQILSVIAGNLF
                     GLEALRTVRLEDVRFPKSILREFKGPKFGIEGMRTILKRPKKPLVGTIVKPKIGLSPK
                     ETAEYVYEAGMGGLTNSKDDETLVNQSFCPLEERVVAIAEALDRVRDESGHVMMHAIN
                     VSTNGHQILEVAERAQELGASELMVDVLTCGFGAVQALAEDPSIKLPIHVHRTMHGAI
                     TRNPDHGIHMKVFATLVRMVGGDALHVGTFGVGKMKGEPHEDLANRDVCLTAELPYKR
                     VMPVASGGVHAGMIKRLVDIAGTELQIQAGGGVSGHPRGVRGGARAMAQAVDAVMAGV
                     PLDVYAKEHVELAEAIQKWGMA"
     gene            8993..10513
                     /locus_tag="PLJ11_01180"
     CDS             8993..10513
                     /locus_tag="PLJ11_01180"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HOO03317.1"
                     /translation="MKLKARYVDVDTGEVTALLHDDDCAELGLREKDRVRLSLNGRVT
                     VAIVTTSDTVINRGEIGLLGTSQLTLEFKDGDLVEVVSASKPESVEFIKRKMDGAELS
                     TEEINAIIADISNRMLSNIELSAYVTALYINGMNMRETVDLTKAMVAGGETITFSNGP
                     IYDHHSIGGCPGNKITLLIVPIMAAAGCKMPKTSSRAISSAAGTSDIVETFANVALTP
                     AQLKEITERVGGVLAWGGSLNLAPADDLIIRAEYPLGIDPHAQLLASVMAKKKAVGAD
                     YLVIDIPMGEGTKVRTMDEAKAYARDFIQLGEQLGIRVECAITYGGQPLGRAIGPALE
                     SIEAISIMEGKKTPSSVIEKTVSICGMLFEMGGEYQGAERARQILESGKALHKFQEIV
                     EAQGGKPGITSSDIKVGQYSVDVISKQSGYVGQILNKELVRVARAAGSPKDKGAGLVL
                     NKKGGNKVDKGETLFTIYAEHEWKLQEALKLAIRLEPVRTQGMILARVPSFSRVAL"
     gene            complement(10496..11251)
                     /locus_tag="PLJ11_01185"
     CDS             complement(10496..11251)
                     /locus_tag="PLJ11_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03318.1"
                     /translation="MKVVALVTEDFRFFYGAVRALKERGQPFVSLGFNDPLPYNVGLV
                     LTTEPERKRVHGRKVIASNDPEWAAKAALALLQGGPFKRLIVGVDPGPRPGIAILGDG
                     KVLFADDLLSPEAVAPAIRDFLSLVRSDELLLRVGDGDPTNRDRIINALWEVAHNIEM
                     VDERSTTRRTGHPDADAAVAIAQAHGPLLPAPPRVAPTEGEVRDIQRQSRIESQGAVT
                     ISASLARKVATGALSLGEAIERQRGKGRSEGDP"
     gene            11355..11870
                     /locus_tag="PLJ11_01190"
     CDS             11355..11870
                     /locus_tag="PLJ11_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03319.1"
                     /translation="MDGAFATLLREGCVLTCRDFLNGEVPALGLEHPARYLSSVIEGG
                     WHEGGYVGRPSLREGEAPFQCVEAFGPGEGERALIVLGLNLSREGTGAALTFLRSSSS
                     PHKALLYLFSFDGTLDEDGIAQAMLEPELLGMVRGTDIVVYLAMMDVRTGMSMLARID
                     GGERSIRYLAH"
     gene            complement(11864..13141)
                     /locus_tag="PLJ11_01195"
     CDS             complement(11864..13141)
                     /locus_tag="PLJ11_01195"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO03320.1"
                     /translation="MAIRGSFSKAKGFDRRVWTLFYGRIISSLGFSVVMPFLSIYLHT
                     ELGIPMTVVGIVLLISAIVGAVGQIAGGELADILGRRKIMIMAMATRCLMFLGLAYVI
                     AGGASLLVITIMVSLSSLAGSFFEPATNALIADVVEPRKRLEAYGLLRIGGNLGWTIG
                     PLLGGLLSMVSYPFLFLISALATGTVALIVFLFVAESISAGSKRQKLSLRDLGRLKED
                     RRFLAFCLISVPLFVMFGQMASNYAVFSTDIIGISNAEVGYIYALNGIMVVTMQFPIS
                     RSVGRYRMTKVMAAGSMMYAIGYGIVGLTPSIGLMVPSWVFSPGFLYLAVCMFIVTLG
                     EMMVSPASMTLVAKMSPEAERGRYQGMYGLVSNFGFSAGPFFGGLLFDAFSNEPVLMW
                     LGIGSFGLIAALGYLALSRAFRDGSDRIDDPDQ"
     gene            13271..13588
                     /locus_tag="PLJ11_01200"
     CDS             13271..13588
                     /locus_tag="PLJ11_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOO03321.1"
                     /translation="MAALGREGMKSALRGDGDAGDMWALLGAYALLNLAVLLMYGMDK
                     GRARRDRRRIPERDLLLAALIGPFGAMAGMRVFRHKTRKARFKLVPLFALLHVALAFL
                     ILN"
     gene            complement(13597..14724)
                     /gene="dnaJ"
                     /locus_tag="PLJ11_01205"
     CDS             complement(13597..14724)
                     /gene="dnaJ"
                     /locus_tag="PLJ11_01205"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOO03322.1"
                     /translation="MAEKRDYYEVLGVDRTASEEEIKKAYRQLARQYHPDVTKEDPQV
                     AEERFKEISEAYEVLMDKQKRDLYDKYGHAGVNSQFQGGGFNWTDFTHQGDIRDIFGD
                     MGFGGGSIFDMFFGGGRSRGPHQGQSLRYDLEITLEEAAEGVKKELTLPLTVKCEACG
                     GTGSKSRREEVCPQCGGKGQVQRVERRGYSQFISLTACPRCRGSGKDIRDPCPECDGG
                     WTRKRQKISLDIPKGVDTGMRLRVAGAGEPSPDGGPPGDLYVVVTVRDHDIFQRDGAD
                     LYTTQEISFPEAALGATIEVPTLDGKKAELVIPPGTQPGEVQRLKGLGMPKMDGYGRG
                     DLYVRLKLVVPKRLTSEQKELLRKFEGGDGSKKRIFSRNRS"
     gene            complement(14733..>15770)
                     /locus_tag="PLJ11_01210"
     CDS             complement(14733..>15770)
                     /locus_tag="PLJ11_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO03323.1"
                     /translation="KHLTLTLTRAKLEELVEPVVTRCRGPMERAMKDANLTPDKIDAI
                     ILVGGPTRMPIIQRFVESVVGKKIQRGVDPMECVAMGAAIQAAVLAGEVKDVLLLDVT
                     PLSLGIETLGGIATKLIERNTTIPTRKSQIFTTAADGQTSVEIHVTQGERPMAKDNTS
                     LGRFTLSGIPPAPRGVPQIEVTFDIDANGIVNVSAKDLGTGKEAKITITASTKLSKEE
                     IDKMVKEAERFAEEDLKNKERVELINQADQLTYATEKALAELGEKASNEERTKIEALI
                     SDLRAEIKKGEAASIKSKMEALQKEFYSISTRIYQSHAEQQAGQEGPKAGPSAKDENV
                     VDADYKIVDDQ"
BASE COUNT         2811 a         4859 c         5419 g         2681 t
ORIGIN      
        1 ccacccggga gatcgtccgg ggggtggaga tggacctcga tgtccgggcg gaggaccagc
       61 gcaaggtgcg ggtgttcctc gggaaggccg tcgccccggg cttcttcgcc tgggcgatcc
      121 cctgcggggc catgatcagg gtcggtctat gtgtgtccga ggggaacggc ccgccgagcg
      181 cttacctccg ccctctcctg gccacgctgg gcttgtccga ggcggagaga gtgagggcgt
      241 attctggggc cattcccctg gggcatatcg cccggacgta cgccgaccgg gcgctggtcg
      301 caggggacgc ggccggcatg gccaagccga tcagcggcgg cggcctcttc acaggcatga
      361 ccgccggccg gctggccgcg gagaccttcc tggaggcgtt ccgagggaac gacctcacgg
      421 ccaggacgct ctcctcctat gaggggaggt ggaaggcggc cttcgggagc gagctgcgca
      481 actcctatct tgtgcgaagg gcgttcgtgc gcatgagcga tcgggagctg gaccgggtgg
      541 gggcccagct ggacacggag agggcgaagg aggtgctggc cacgggggac atcgacttcc
      601 ccacagccct ggccccgggg ctcctgcggg cctgcccctc cctcatggcc ctcgccccgg
      661 ggctcctgct ccgccttctg aggaggtgag gcatgagggc cctgaaggtg cccaagcggg
      721 aggcggacgc cgtcaaggac cgcctcaacc gcctcggtca actggacctg gggaggaggc
      781 cgggcaggca ggggggctcg gtgcttttgc cggtaacggg ggagggaccc ttccccccgt
      841 atccgttggt ggacgtcgag ctggagccgc aggaccgcca ccgtgctccg caggagagga
      901 tcgccgagcg cctggacctc cccggggagc tgaaggaact gctgccgaac aagtatgagc
      961 gcctcggcca cgtcctcgtc ctcaggctcc ccgggcccct gctcccgtac cgtcatgcca
     1021 tagcggaggc gtacgcccat gtgctcaagg cgaggacggt gctcctcgag cgggggacca
     1081 tccacggcgt ggagcgccgg ccggacatgg agctcctcta cggcacggag acggagaccg
     1141 tccatctgga ggcgggcata aggtacgcat tggaccccgc ccgggtcatg ttctcctcgg
     1201 gcaacttcga cgagaagcgg cgcatggcct ccctggactg ccggggggag accgtggtgg
     1261 acatgttcgc cggcatcggc tacttcaccc ttcccctggc ggtgcaggcc agggcggacc
     1321 gggtgatcgc ctgcgagatc aatccggagg cggtgcgctt ccttcgcagg aacctgcggc
     1381 tgaacggggt gggggacagg gtgaccgtct tcgaggggga caaccgggac ctcccgggcg
     1441 aggggttcgc ggaccgggtg gtgatggggt acgtgaacgt gacgtggcag ttcctgccca
     1501 aggccttctc gctggtaagg aggggtggca tactccacta tcacgacaca tgctccatcg
     1561 accgcatccc agaagggttg ctggcgaggg tgcgcgaggg cagcggcggc cgtccctacg
     1621 aggtgctggg cataagggag gtcaaggcct acgccccctc catctcccac atggtcgtgg
     1681 acatcagggc gctctgagcg caacttcccc ggagccggac tcggccaata gggccgagag
     1741 cgcctcatgg tacccctcgc cggtggagga gagcattcgc gacgcgtgcc gggcaaggtg
     1801 ctccccggac tccctctcct ccttgttgaa ctgccatccc cgggactgtg cctcccccac
     1861 agccgtcaag aaggcgtagc agaccgccct gtgcgtgtgc tccttttcgg tgcgctccac
     1921 aagccggagc atcgcggcgg tcccctcgat ccggtaggcc ttggcaaggg agtcggccag
     1981 ctgccccacc gggccaagcc ccgagtaggc ggggaggttt cccagtacct ccctctccct
     2041 gaccagccta tccgcctccg agagcatctg gcccggggtg agctccctca tccccaggac
     2101 cctttgtgcg attccctcgt ccgaaacggc ggtaagcatc gttcgggcgt gggcctccca
     2161 cagctccccg atgaccttcc ccgcctggtt ctgctcggtg acgatgggga tgccgaggga
     2221 caggacagcc cgcagggcgg tccgctccag gtcggtcccc ttcagggccc ccaggaaggc
     2281 ctccccgtcc ttgccgtagc attcctggcc caggacgtac acctggcccc gtgagcgcat
     2341 ccattcgccc ttggccttga tatgggcagt gatgaggccg gcatcgtcaa ggtccccctc
     2401 cagatattcc ctgaccaggg acgccggggt ggggaactcc ccttggcatt ccggtcgggc
     2461 ccctccgtac ctgtggtcca cgctgaccct caggtcatcg taggggtcgg gggcggccac
     2521 ccgggccatt aggtgctggg cggtgttgac gcccgcaccg cactctgcgc agaccctgat
     2581 ctcgacgttg gccgagagcc attggatgcg gacggccggc ctgtccccat gcccgcatcc
     2641 gctgccctcc agccgatagg ggaggttggc caggacctcc ttgacgtagg cctcgggggc
     2701 cttcggcccc gagacggagg cgaagagacc cccctccgtc gaatagatgt gcaggcgggc
     2761 ctcccgggcc atgtcccagt acgccagaag ccggaggtcc gggtcgtcga agtgctgcag
     2821 gcctatgagc ttctccttgt ccaccgtgcc ccggatggcg aaggagacgg ggccgatcgg
     2881 caggttggcg gtggtgagat aggggagctt tccggaggcg gcgagggaga tcgccgccgc
     2941 atacgccctt acgaactggt cccccttgct ggcgagcttg atgagccttt ccgggtcgtc
     3001 cctgtatcta agcacctttg cgatgccctt ctcgatgtgg gcgaacgggg acctccgtcc
     3061 ctcctccagg aggaccggga taacgagcga cgggtcatcg gctagcctct gggatgttgc
     3121 cagcatctcc ttctccatgt tcttgggcgt cttcttgatc ccgcggaagc gcggccgggg
     3181 aggcataggc cctcatctcc ctctgcgcat taaaccattc ggcgctagtt aattatgcct
     3241 ccaatgccat gaggtacatg gcgagggatg ggttgggcag agagtacgct gatggctatc
     3301 tgaagggata cgaggacggg ctgaaggagg cattggagca gcttatcgca acgacctgcc
     3361 ggaagaacta cagcagcact gagatccagc tcctggtcaa gaaccagcgg gcttccgtgc
     3421 cgaagaagat ggccctgcgg aggaggcagc ttgagcggga gctcggactg gacctggaaa
     3481 ggaaggagga ggggccagag ccgaggcggg aggctgcccc ggggaggacc gtgctcctca
     3541 agggggaggg caaccaggag gcactggagg ccttccggga cctggtccag gcggggtggg
     3601 atgggctgtg catctcccgt atgtccccca gggacgtgcg gcagttcgtc gggaaggatt
     3661 gcacggtgct ctggctcacc aaggccgatc ccatggccgg ggaccaggac cgggaggagt
     3721 accttcagcc cacggagacg gccaagatgc agacgagcat ccgaaacttc ctgagcaaga
     3781 ataaagggag gccgtcggtc atcctgctgg atgggatgac ctttatcgtg accaacaccg
     3841 acttccgctc cttcctgaag atcgcccaga agctgaagga tgacgtctac caggcgcgct
     3901 ccctgctcct cctcccctat gagccgaaga acctcctgga gaacgaggtg aacctgttca
     3961 taaacgagat gtcctagggc cggttgaggg cgatcttgat ggaggaaatg ttctggggcg
     4021 caccgtccgg accgggcagc acctcggtgc tgatgcttat cccgcccagc ttgacgctgt
     4081 ccttggagcg gtccagggtt atcagggcga cgtccacggc cttgctgatg cacttgcccc
     4141 gggcctgcac caatatgctt gctccaggct cagcgagctt ctttcccgct gccaggacgt
     4201 agttcatgac aggcttctga ccgatgtata tgacgctctc gcccatggta ggcccctcgg
     4261 gatcttccat tttgtttagg tgggaagagg ataatcacgg tttcggtaca gccgtagaaa
     4321 tgcagggcaa tgggaccgcc cccgtcagag aacggccgtg gaaggggctc ggaccctccc
     4381 ttggtgcatc ggccctaatg cgaggcgcct cggtcgaagc taggaccaca ggcccctaga
     4441 aaactatacc taggaaggag ggagatagag tgctgggcca gcggcccgtc cgagggggat
     4501 acgatgcagg agaatgaact tagcttgacc agcgaggata tgcaggacat ggccaaggag
     4561 gccatcatca tctggtggag cacggtgatg aaggcggtgg tgatgagcag caacactccg
     4621 gagatgagga ggatcctcca tagcttcctt gccgaggcgg tcaccgagat ggtcaaggtc
     4681 aatgggtccc agaggaccct ggagctcatg gggccgatca tgaggcaggg gggcaagaag
     4741 gcggcggtga gggcgctcga ggagcatgag atcgtggacc gggatggcat tgcgatctcc
     4801 tctctcatcg acctctggga gggggcgctg ggcgtccggg gggagatcgt ggagaggagc
     4861 gagacatcgg tggtcaaggt ggccaagcag tgcccgttcg ccaattcccc tccggagctg
     4921 tgctccctct tcggcaggtt cgtggacggc ttctgcgagg tcatcaatcc ccagtatggg
     4981 tggttccaga cccataggat gtccgcaggg gacgaggaat gccgctgggt atgcaggaag
     5041 gagggttgag ggcgtaggtg gagaacaacg ccaaggtagc ttccatattg gaggaggtgg
     5101 ccgacctcct ggagctgaag gaggacacct tcttcaaggt gcgggcctat cggcgggcgg
     5161 cgaaggagat cgccgctctc accgaggaca tcaaggacat atacataaga ggccacctgg
     5221 acaaggtgcc gggggtcggg agggcgatcc atgacaaggt cgtggagata ttgcgcacgg
     5281 ggcagctcca atacctgaac gacctgcgga acgagttccc ggccgggctg ctgcaggtca
     5341 tgcaggtgcc ggacataggg ccgaagaccg cagcccggtt gtacaaggag ctgaggatca
     5401 ccaacctcca ggacctgaag gaggcggcgg agcagcaccg catccgcatg ctcaagggct
     5461 tcggggagaa gacggaggag aacatcctga agggcatcag gtacctggag tcgcggcagg
     5521 ggaggatgct gcttggctat gccttcccaa ggggcagggc cttggaggac catatgcgaa
     5581 agcaggggtt cgcccttgtc agcctgggcg ggtcgctccg gcggatgaag gagaccatcg
     5641 gggacatcga catcctggtc gggagctccg agggcgaacg ggccatggac gcgttcgtct
     5701 cccatccgaa cgtggcggag gtgctcctcc gcggggagac gaagacctcc atccgcctgc
     5761 aggacggggt gcaggtggac atgcgggtcg tcgccccctc gtccttcggg gcggccctcc
     5821 aatacttcac gggctccaag gagcacaacg tgaacatgcg gtccctggcg agcgagctcg
     5881 gcctcaaggt caatgagtac ggggtgttcc gcttggtgga cggggccagg gtcgccgggg
     5941 agaccgagga ggaggtgtat gcggccctgg ggatgcaatg gatgcccccg gagatgcggg
     6001 agaaccgggg ggaggtcgag ctggccagga aggggcaggt gcccaaggtg gtcggccttc
     6061 ccgacatccg gggggacctg cacatccaca gcgaggccag cgatggggtg gacggcgtcg
     6121 gggccttggc cgccgcggcc gaggcccgtg gctatgatta cttggccatc acggaccata
     6181 gccagtccct gaccgtcggg aacggtctca gcgtggagcg gatgctggag aacatcgggc
     6241 acatccgtac cgttcaggag gagcatccag gcgtccacct gctggctggg gcggaggtgg
     6301 agatcgatgc gaaagggggg cttgactacc cccaggggct gctggaggag ctggacgtcg
     6361 ttgtggcggc cgtgcactcc catttcaaga tggcccctaa ggagatgacc gaacggctgc
     6421 tgagcgccct ctccaatgag acggtggacg tcctggccca cccgacaggc cgcatcatcg
     6481 gggagaggga ggggtacgga ttcgacatgg agagggtgat gcgggcggcc aaggacaatg
     6541 gggtggcgct tgaggtcaac tctttcccgg agcggttgga cctgaacgac gtccaatgcg
     6601 ccattgcccg ggagagaggg gtgaccgtat gcatcaacac cgatgcgcac aacgtccgcc
     6661 agctggacaa tatggtgtac ggggtggcca ccgccaggcg gggatgggtg cctccgggga
     6721 tggtgctgaa cgcccttcca tggaccgccc tgaaggaccg cctcgacctg tgatgctgga
     6781 cggggaacgt ttatcaatgt gcaggaccga tactcacagg atgtcggccg aggatgtggc
     6841 ggagcgtata cgctccatgg agatacgcgg ggcggctgag atagccagga gggccgcgct
     6901 tgccctgagg gaggaggccg aggcctatga ggggaacgac ctcgaggggc tgcgctcgca
     6961 cctagaacgc gccaaggagc tgctgctggc ctcgcgtccg accgcgatat ccctctggaa
     7021 cggcgtgcag tatgtgttca aggacacctc caaggcgacc acggcggaag agtaccgaag
     7081 caaggttagg gagaacgcgg agcatttcgt caagcgctcc caaagcgccc tgaggaccat
     7141 cggcaggctg ggcgccaacc gggtccggga gggggacagg gtgctcacgc actgcaacag
     7201 caaggccgcc atagcggtca tggccgaggc atgggcgcag gggaagcgct tcgaggtcta
     7261 cgccaccgag tcccggcctt ggaggcaggg actgctcacc gccaacgacc tggccaggct
     7321 gggcatcccg gtgaccatga tcgtcgattc ggccgtgcgc tacgtcatga aggacatgga
     7381 cctggtgctt gtgggcgcgg acaccatcgc ctccaacggc gccctgatca acaagatcgg
     7441 aacggcgcag gtggccttgg cggcgcatga ggcgcgcgtc tcgttcacgg tctgcgcgga
     7501 gaccttcaag ttctcccccc ggacgatcta cggggagctg gtcaagatcg aggagcgcga
     7561 ccccaccgag gtcgtgaagg agggggaggt ccctccgcag gtcaaggtct tcaacccggt
     7621 gttcgacgcc acgccgcccg agtacataga ctccatcgtc accgagatcg gggtcatccc
     7681 cccctatgcc gcctatcagg tgatcatcaa ggagctggga caggagttca tcttccacga
     7741 ggaatgagag catggaatac gaggagagat atctacatct gaacgagccg atcgatgccg
     7801 atgcgcacgt catatgcaag tacaaggtcg ggaccgacct gaagatgacc accgccgcgg
     7861 cggcgatcgc caccgagcag tcgaccggga cctggaccaa gctgtccacc ctgagggagg
     7921 agaacttcca aaggaggagc gggaaggtga cggccatcga agggaacgtg gccaccatcg
     7981 cctatcccgt ggaggacttc tccctggata tagggggcgt gccccagatc ctgagcgtca
     8041 tcgccggtaa cctgttcggg ctggaggccc tgcgcacggt ccgcctggag gacgttcggt
     8101 ttcccaagag catcctcagg gagttcaagg gcccgaagtt cggcatcgaa ggcatgcgca
     8161 ccatcctgaa gaggccgaag aagcctttag ttgggaccat tgtaaagccg aagatagggc
     8221 tctcgcccaa ggagaccgcc gagtacgtct atgaggcggg gatgggcggc ctgaccaaca
     8281 gcaaggacga cgagacgctc gtcaaccaga gcttctgtcc cctggaggag cgggtggtgg
     8341 ccatcgcgga ggcgctggac cgggtgaggg acgagagcgg gcacgtgatg atgcacgcca
     8401 tcaacgtcag caccaacggc caccagatcc tggaggtggc cgaacgggcc caggagcttg
     8461 gggcgagcga gctgatggtg gacgtgctga cctgcggctt cggggccgtc caggccctgg
     8521 cggaggatcc gtccatcaaa ctgcccatcc atgtccatcg caccatgcat ggggcgatca
     8581 cccgcaatcc cgaccatggc atccacatga aggtgttcgc caccctggtc aggatggtgg
     8641 gcggggacgc cctgcacgtg ggaacgttcg gggttggcaa gatgaagggg gagccccatg
     8701 aggacctggc caaccgggac gtgtgcctta cggccgagct gccgtacaag agggtcatgc
     8761 ccgtggcctc gggcggcgtg cacgcaggca tgatcaaacg gctcgtggac atcgcgggca
     8821 cggagctaca gatccaggcg ggaggggggg tctccggcca tccgcgcggc gtgcgcggag
     8881 gcgcgagggc catggcccag gcggtggacg cggtgatggc cggcgtcccg ttggacgtct
     8941 atgccaagga gcatgtcgag ctggccgagg ccatccagaa gtgggggatg gcatgaagct
     9001 taaagcccgg tacgtggacg tggacactgg ggaggtgacc gccctcctgc acgatgacga
     9061 ttgcgccgaa ctcggcctgc gggagaagga ccgcgtgcgg ctctccctga acggaagggt
     9121 cacggtcgcc atagtcacga cgagcgacac ggtcatcaac cgaggggaga tcgggctgct
     9181 ggggacgtcc cagctgaccc tggagttcaa ggacggggac ctggtggaag tggtctccgc
     9241 cagcaagccc gagtccgtgg agttcatcaa gaggaagatg gacggggcgg agctgagcac
     9301 cgaggagatc aacgccatca tcgcggacat atccaaccgc atgctgtcca acatcgagct
     9361 ctccgcctat gtgaccgcgc tctacatcaa cggcatgaac atgcgggaga ccgtggacct
     9421 caccaaggcc atggtggccg ggggggagac gatcaccttc agcaacggcc cgatctacga
     9481 ccatcacagc atcggcgggt gtccggggaa caagatcacc ctccttatcg tcccgatcat
     9541 ggcggccgcg gggtgcaaga tgcccaagac gtcgtcccgg gccatcagca gcgccgccgg
     9601 cacctcggac atcgtcgaga cgttcgccaa cgtggcgctg acgcccgccc agctgaagga
     9661 gatcaccgag cgggtgggag gggtcctggc ctggggaggg tcgctgaacc tcgcgccggc
     9721 ggacgatctc ataatcaggg cggagtaccc gctcggcatc gacccgcacg cccagttgct
     9781 cgcatccgtg atggccaaga agaaggccgt gggggccgac tacctggtca tcgacatccc
     9841 catgggcgag gggaccaagg tccgcaccat ggacgaggcc aaggcctacg cccgggactt
     9901 catccagctg ggggagcagt tggggattcg agtcgagtgc gccatcacct acgggggcca
     9961 gcccctcggc agggcgatcg ggccggcgct cgagtccatc gaggccatat ccatcatgga
    10021 ggggaagaag accccaagca gcgtcataga gaagaccgta agcatctgcg gcatgctctt
    10081 cgagatgggc ggggagtacc agggcgcgga gagggcgagg cagatcctgg agagcggcaa
    10141 ggccttgcat aagttccagg agatcgtgga ggcccaggga gggaagccgg gcatcacctc
    10201 cagcgacatc aaggtggggc agtacagcgt ggacgtcatc tccaagcaga gtgggtacgt
    10261 ggggcagatc ctgaacaagg agctggtccg ggtggcgcgc gccgccggct cgccgaagga
    10321 caagggggcg ggcctggtcc tcaataagaa gggcggcaac aaggtggaca agggggagac
    10381 cctcttcacc atctacgccg agcacgagtg gaagctgcag gaggcattga agctggccat
    10441 ccgtctggag cccgttagga cgcagggcat gatcctagcg agggtaccct ccttctcaag
    10501 ggtcgccctc tgatctcccc ttccccctct gcctctctat ggcctccccg agcgagagcg
    10561 ctcccgtggc caccttccgg gccagcgagg cggagatggt cacggcgccc tggctttcga
    10621 tgcggctctg ccgctggatg tccctcacct ccccctcggt cggggcgacc ctgggagggg
    10681 cggggagcag ggggccgtgg gcctgggcta tggccaccgc ggcgtccgcg tcgggatggc
    10741 cagtgcggcg ggtggtggac cgctcgtcca ccatctcgat gttgtgagcg acctcccaga
    10801 gcgcgttgat gatccggtcg cggttggtcg gatcgccatc ccccaccctg agcagcagct
    10861 cgtcgctgcg caccagggag aggaagtcgc ggatggcggg ggccaccgcc tcgggggaga
    10921 ggaggtcgtc ggcgaacagc accttgccgt cccccaggat ggcgatcccc ggccgtgggc
    10981 ctgggtccac cccgacgatg agacgcttga aggggcctcc ctgcagcagc gccagggcgg
    11041 ccttggccgc ccattccggg tcgttgctgg cgatgacctt gcggccatgc accctcttcc
    11101 tctccggttc cgtggtgagc accagcccca cgttgtacgg cagggggtca ttgaagccta
    11161 gggagacgaa gggctgcccc ctctctttca gggcgcggac cgccccgtag aagaagcgga
    11221 agtcctccgt gaccaaggcc acgaccttca tcggacctta acctgatcgc atcgcatata
    11281 atccttggtc ctgtttcgcg cctcagggcg gaaatgggac aagaaggaaa taggaggggg
    11341 tatgatccga acacgtggat ggcgccttcg ccaccctcct gagggagggg tgcgtgctga
    11401 cctgccggga cttcctgaac ggagaggtcc ccgccctggg gctcgagcac cctgcccggt
    11461 acctttcttc ggtgattgag ggaggatggc acgaaggcgg gtatgtcggg aggccgagcc
    11521 tgcgcgaggg cgaggcgccc ttccaatgcg tggaggcctt cggcccgggg gagggggagc
    11581 gcgccctcat cgtgctcggg ctcaaccttt ccagggaggg gacgggggcg gcgctgacgt
    11641 tcctgcgctc ctcctcctcg ccgcacaagg cgttgcttta cctcttctcc tttgatggga
    11701 cgctcgacga ggacggcatc gcccaggcca tgctggagcc ggaactgctg gggatggtca
    11761 ggggcaccga catcgtcgta tacctggcaa tgatggacgt ccggacgggg atgagcatgc
    11821 tggcgaggat agacggagga gagcgcagca tccgatatct tgctcactga tcggggtcgt
    11881 cgatgcgatc gctgccgtcc cggaacgccc ggctcaaggc caggtagccg agcgcggcga
    11941 tgaggccgaa ggagccgatc cccagccaca tcagcacggg ctcgttgctg aaggcgtcga
    12001 agagcaggcc gccgaagaac ggccccgcgg agaagccgaa gttgctgacc agcccgtaca
    12061 tgccctgata cctccccctt tccgcctccg gcgacatctt ggccaccagg gtcatggagg
    12121 cgggggagac catcatctcg ccgagggtga cgatgaacat gcataccgcc aggtacagga
    12181 accctggcga gaacacccag ctagggacca tcaggccgat gcttggggtg aggccgacta
    12241 tgccgtaccc tatggcgtac atcatcgaac cggcggccat gaccttggtc atccgatacc
    12301 ggccgacgga acgggagatg gggaactgca tggtcaccac cattatcccg ttgagggcgt
    12361 agatgtagcc cacctcggcg ttgctgatgc cgatgatgtc ggtggagaac accgcgtagt
    12421 tggaggccat ctgcccgaac atgacgaaga gcgggaccga tatcaggcag aatgctagga
    12481 agcggcggtc ctccttcaac ctccctaggt cccggaggga gagcttctgc ctcttggagc
    12541 ccgcggagat gctctccgcc acgaacagga agacgatcag cgccaccgtc ccggtcgcca
    12601 gggcgcttat gaggaacagg aagggatagg acaccatgga cagcagtccg cccaggagcg
    12661 ggccgatggt ccaccccagg ttgccgccga tgcgcagcag cccataggcc tccaggcgtt
    12721 tccggggttc cacgacatcg gcgatcagcg cgttggtggc cggttcgaag aaggaccccg
    12781 caaggctgct cagcgacacc atgatggtga tgacaaggag gcttgcccct ccggcgatga
    12841 cgtaggctag cccaaggaac atgaggcagc gcgtcgccat ggccattatc atgatcttcc
    12901 ggcggccgag gatatcggcg agctcccctc ccgctatctg gcccacggcc cctacgatgg
    12961 ccgatatcag gagcacgatg cccacaacgg tcatcgggat gcccagctcg gtgtggaggt
    13021 agatcgaaag gaacggcatc accactgaga agcccaaaga ggagatgatg cgcccgtaga
    13081 agagcgtcca gacccggcgg tcaaagccct tggccttgct gaacgacccc ctgatggcca
    13141 tgttcaggcc ctagtcatgg ccaagcacat cataatcgtt tccgatgaca cctggtagca
    13201 cggccccgag cgctcagggc gctgaacatc tcggaccgtt gggacctcat tggcatgggc
    13261 gggccaaggc atggccgccc ttgggagaga ggggatgaaa agcgctttac ggggcgatgg
    13321 cgatgccggg gacatgtggg ccttactcgg ggcgtatgcg ctgctcaacc tggccgtcct
    13381 gcttatgtat ggcatggaca aagggcgggc caggagggac cggcggcgca tccccgagag
    13441 ggacctgctg ctggcagcgc tcatcggccc gttcggggcg atggccggca tgagggtgtt
    13501 caggcacaag accaggaagg cgcggttcaa gctcgtgccg ctcttcgcgc tgctgcacgt
    13561 tgcgttggcg ttcctcatcc ttaattgatg caacgctcag gatctgttgc ggctgaagat
    13621 gcgcttcttc gagccgtcgc ccccttcgaa cttgcgcagc agttccttct gctcgctcgt
    13681 cagcctcttc gggaccacca gcttcaggcg gacgtagagg tccccgcggc cgtacccatc
    13741 catcttcggc atccccaggc ccttgaggcg ctgcacctcg cccggctggg tgccaggcgg
    13801 gatgactagc tccgccttct tcccgtccag ggtgggcacc tcgatggtcg ccccgagggc
    13861 cgcctccggg aagctgatct cctgggtcgt gtagaggtcg gccccgtccc gctggaagat
    13921 gtcatggtcc cggactgtga cgacgacgta caggtccccg ggcgggcccc cgtcagggga
    13981 aggctccccg gcgccggcca ccctcagccg catgcccgtg tccaccccct tcgggatgtc
    14041 cagggagatc ttctgccgct tcctggtcca gcccccgtcg cattcggggc aggggtccct
    14101 gatgtccttg ccggagcccc tgcacctcgg gcaggcggtc aatgaaatga actgcgagta
    14161 cccccgccgc tccacgcgct gcacctgccc cttgccgccg cactgcgggc agacctcctc
    14221 cctgcggctc tttgagcccg tgcctccgca ggcctcgcat ttgacggtga gcgggagggt
    14281 cagctccttc ttgacgccct ccgcggcctc ctccagcgtt atctcgaggt cgtaccggag
    14341 ggattggccc tgatgggggc cgcgggagcg ccccccgccg aagaacatgt cgaagatgct
    14401 cccgccgccg aagcccatgt cgccgaagat gtcacggatg tcgccctggt gggtgaagtc
    14461 ggtccagttg aaccctccgc cttggaactg gctgttcacg ccggcgtgcc cgtacttgtc
    14521 gtacaggtcg cgcttctgct tgtccatcag cacctcgtac gcctcggata tctccttgaa
    14581 gcgctcctcc gccacctggg ggtcctcctt ggtgacgtcc gggtggtact ggcgggccag
    14641 ctggcggtag gccttcttga tctcctcctc gctggccgtg cggtccacgc ccaggacctc
    14701 gtagtagtcg cgcttctcgg ccatcgggtc gcctactggt cgtccactat cttgtagtca
    14761 gcgtcgacca cgttctcgtc cttggcggag ggcccggcct tgggcccctc ctgccccgcc
    14821 tgctgctccg cgtggctctg gtagatgcgg gtggagatgg aatagaactc cttctggagc
    14881 gcctccatct tgctcttgat cgaggcggcc tctcccttct ttatctccgc cctgagatca
    14941 ctgatgaggg cctcgatctt ggtcctctcc tcgttggagg ccttttcgcc cagctctgcc
    15001 agggccttct ccgtcgcata tgtgagctgg tcggcctggt tgatcagctc caccctctcc
    15061 ttgttcttca ggtcctcctc cgcgaagcgc tcggcctcct tgaccatctt gtcgatctcc
    15121 tccttggaga gcttcgtcga ggcggtgatg gtgatcttgg cctccttgcc agtgcccagg
    15181 tccttggcgc tgacgttcac gatgccgttg gcgtcgatgt cgaaggtgac ctcgatctgc
    15241 gggacgcccc tcggggccgg ggggatcccg gagagggtga agcggcccag cgacgtgttg
    15301 tccttggcca tcggcctctc cccctgcgta acgtggatct ccacgctggt ctgcccatcg
    15361 gccgcggtgg tgaatatctg cgatttgcgg gtggggatgg tggtgttgcg ctcgatgagc
    15421 ttcgtcgcta tcccgccgag ggtctcgatg cccagggaga gcggggtaac gtccagcaga
    15481 aggacgtcct tgacctcccc ggccagcacg gcggcctgga tcgcggcccc catggccacg
    15541 cattccatgg ggtccacccc ccgctggatc ttctttccca ccacgctctc cacgaaccgc
    15601 tggatgatcg gcatgcgcgt cggcccgccc accaggatga tcgcgtcgat cttgtcgggc
    15661 gtgaggttcg cgtccttcat cgccctctcc atggggccgc ggcagcgggt gacgaccggc
    15721 tcgacgagct cctccagttt ggcccttgtc agggtgagcg tcaggtgctt
//
LOCUS       DAOSYN010000013        15213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_3273_length_15213_cov_4.405792, whole genome shotgun sequence.
ACCESSION   DAOSYN010000013 DAOSYN010000000
VERSION     DAOSYN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 15213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15213
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3273_length_15213_cov_4.405792"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLJ11_01215"
     CDS             complement(<1..792)
                     /locus_tag="PLJ11_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOO03324.1"
                     /translation="MLGPLRERGYDARSLSVSHLDEVREDVESLHREGLISEELYKEY
                     LFRFKYYVPSAFPEARSIVVVSVPQPTCEVVFRVGGRRRRAIVPPTYANAVEVDMEIV
                     DLLEKGDGRGVRLMRAILPHKTLAARTGLAAYGRNNITYVPKYGSYHRLTSFFTDREL
                     PDDWQEMRMMERCEGCMACVDACPTAAIVRDRFLMFGERCLTFHNEMPNERGFPDYIM
                     EGMHNAVIGCMICQMVCPVNAQVRNWMVHCTEFDEEDTEYLLKGDF"
     gene            908..2143
                     /locus_tag="PLJ11_01220"
     CDS             908..2143
                     /locus_tag="PLJ11_01220"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HOO03325.1"
                     /translation="MKEVLDRALRVQDIDLCDHCLGRLFAQVGTGTTNLNRGQELRAL
                     IDQDRASRGLGPMRHTRCSVCAELFDQVPRFAGAVALKLQEVECDNFLVGTRVDPIVQ
                     AREEGLWSIVGQEHAEPIKSELNREIGKAVSALNGKEVEFKSPQVVALVDTRFAHVEL
                     DVAPLFIYGRYRKLSREIPQTKWPCRACQGKGCPRCANTGKMYATSVQEQIGDVLLPY
                     AKAKGHFFHGMGREDIDALMLGTGRPFVIELSSPVIRHLDLPALEREINERGKGLVEV
                     EGLRPSDREEVRRVKNATPEKEYRAQVAIHGKVNKERVDEVLRTFNRTRIVQRTPVRV
                     AHRRADLERERHIVYVVLEDFQGDRMTLRLRTESGTYVKEFVHGDQGRTQPNLSEMLG
                     VPCTVEALDVIGVIDSSEG"
     gene            2146..2442
                     /locus_tag="PLJ11_01225"
     CDS             2146..2442
                     /locus_tag="PLJ11_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HOO03326.1"
                     /translation="MVKASHGLRRKGRKVLKKSPRARGLSPITHEFVQFEVGEKAHIH
                     LDPSIVRGAPHVRFHGKTGTVMAAQGRAYVLSVKDGNKLKTVISRPEHMRKVKN"
     gene            2448..2786
                     /locus_tag="PLJ11_01230"
     CDS             2448..2786
                     /locus_tag="PLJ11_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03327.1"
                     /translation="MRMSKDRYVTLAEVKEMLEAESKAREFTPEQKLALEHAKDTKLS
                     AKEARELVEKLLELEFIADLNNAEAVAVKITDLLPTHFDDVRLIFQKERRVLDKKQGE
                     QIIKLVNEYL"
     gene            2799..3347
                     /locus_tag="PLJ11_01235"
     CDS             2799..3347
                     /locus_tag="PLJ11_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HOO03328.1"
                     /translation="MEDYAHILDYLPQGLPTERGFRREPVAYALGSTELKLFELVPKP
                     GAIITPGEVVYIGKDMDKRDKILHVKRRVGYEELNAGSQNELPFVILEVVKENEPRFV
                     QFFNEAESITTRYHVLELLPGLGKKTMWAILEERKKAPFKSFADIAARIPTFKHPEKV
                     VAKRIEVELADPSQKYRVFVPR"
     gene            3352..4134
                     /gene="rsmA"
                     /locus_tag="PLJ11_01240"
     CDS             3352..4134
                     /gene="rsmA"
                     /locus_tag="PLJ11_01240"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOO03329.1"
                     /translation="MSIGELRSLLARYGVRPSKAKGQSYLIDRRVAEREAEFLGIAPG
                     DRVLEVGPGFGTLTRALLDRTDMVTAIELEEGAVRYLRETFPRLEVIQGDFLAIDVPR
                     FDLFISNVPYSISSPLLFRLLEMDFRRAVLMVQREFAERMVASAGDDAYSRLSVNVYY
                     RAECRLLENVPRSRFWPEPEVDSAIVMLTPRPAPFRVRDERRYLELVDTLFQQRRKKI
                     GTVLRQRGMLPDDRSNVPYLDERVERLAPAQIAELSDALTGH"
     gene            4202..4978
                     /locus_tag="PLJ11_01245"
     CDS             4202..4978
                     /locus_tag="PLJ11_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOO03330.1"
                     /translation="MSSAEGTMDIAEMGGTLKRAGHLRLEPLCIYESKERPEGGRPFG
                     DVDRCIAKAVLMCAKGEEPLLYISADARAGVCPGGQSWLGLTEMAEGLKYFISTGSPA
                     FRGGEAEHLKRDPELVTRSKAFVGRVRPPQAHLCVAQCARYRQGTGQPRAILLFAEAE
                     QLRNLLALHHFGTPDAFTSTSAPWGPSCASFLTYPAGLASNCPDQALIIGPVDPTGNR
                     WLPPDLMSLGIKVRDAERMARDVPASFLTKRPQVAFPDGR"
     gene            5043..6233
                     /locus_tag="PLJ11_01250"
     CDS             5043..6233
                     /locus_tag="PLJ11_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOO03331.1"
                     /translation="MLIGIVGKPNVGKSTFFAAATMATAEIANYPFTTIKPNKGVGFV
                     RSRCPHLDFNVKCQPRNSACENGVRLVPIELLDVAGLVPDAHLGKGLGNQFLDDLRQA
                     DAFIHIIDASGSTDAEGNAVRPGSHDPMEDVIFLEREINQWIKGIMIKGFDKLARGAH
                     LEGQKVEQVVHDRLTGLGVTEAQVSAALRLSDMPQRIIDWQDEDLYRLAANIRRLSKP
                     MVLALNKADAADPETIRRLSELEGYVAVPTCAETELALRRAAKAGLVSYTPGDPSFKV
                     NDPSKLSAGQRKALDYMARAMERYGGTGVQRCLEKVAYELLEMLVVYPVEDEGHLTDH
                     DGHVLPDAFLLRRGSTARDLAYKVHTDLGDNFIRAIDARTHRVVGHDHPLKDGDVMTI
                     VAKR"
     gene            6237..8672
                     /locus_tag="PLJ11_01255"
     CDS             6237..8672
                     /locus_tag="PLJ11_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HOO03332.1"
                     /translation="MGEWDVRLLTASYSRGENDNIVVELFGKTRDGRSITLRRTGFRP
                     YFHVAAPPEEVLPHVQGKDDLLAVEPVDLFLINKGRVQRCSKVVIRFPWAVPQYRAEL
                     KRQGMEVYAADIPFHHRFIYDNDLPACFTARGEEVKDRYTTDLVVNLQGFTEVPPFTP
                     KLKILSFDIENSITDETIYTICYVIKDEAGTRAGEPITGGEREMIERFTEVIQMEDPD
                     VITGYNIDGYDIPYIIERARKLGLNELKWGRDGSSPRRVMNRFWRCTGRLIVDAWWAV
                     KVEIRPKQETLNAVSKALLGEEKLDVDMKMKVGDDYDPRAIDKEWTNDRDKVLAYCTR
                     DAELALKVLGKVGRIRKGMDLAAVSRMPVDDVLNSGSSQLIDAIMIRAADRHVPQVAV
                     PLTGSFEDEEEAIEGGYVHEIAPGVYHWVCVLDFKSMYPSLIIAKNICFTTRDDRGTI
                     VSPLGVRFLSKEQQPGILPTILADLMRRRDETKAMAKRAKDPEEAHYYEGLQAAIKVL
                     MNSFYGVFASSFYRFTDRSIGGSITAFARETTKGIIKALTDEGVNVIYSDTDSVFVQS
                     PHQDLEGSLAFGRELAARFSKEGGSLEFEKLMEPLFSHGKKKRYVGRMVWPKEELVIR
                     GYEVRRTDAFDLQSEALMTVFEFIMDGKTEEAVKAARGYVQDTLAGKAPLEKLVISKG
                     CRAFKDYANPDTQATVQTARKLIDMGYEFVPGMKVSWIVTNSRKRPQEVQPYISGKEF
                     GYTPDYRYYAERLAQTLARATEVFGWGEKDLMNGNQQASLFDYGSEEGQRPKVRLEQV
                     KKTDRKLTLDDYF"
     gene            8802..9440
                     /locus_tag="PLJ11_01260"
     CDS             8802..9440
                     /locus_tag="PLJ11_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HOO03333.1"
                     /translation="MVDNKTDDSGEGPRPVSGRSMYSYISEAWDRPDDSYVNELMWER
                     LILWRREPNFSRLERPTRLDRARALGYKAKQGIIVVRGRVRKGGLQRRKIWKGRRAKR
                     KGMTKITTGKSLKRMAEERAAKRYPNMEVLNSYWVGEDGKNVWYEIILVDKHHPSIIA
                     DKDLNWICGSAHKNRVFRGKTSAGRKGRGLHWKGKGAEKARPSVRANDHHIK"
     gene            complement(9450..10301)
                     /locus_tag="PLJ11_01265"
     CDS             complement(9450..10301)
                     /locus_tag="PLJ11_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOO03334.1"
                     /translation="MGLLLVRYSELGLKGPGVRRYFEKVLIRNMMAALAKERVEALIE
                     GQYGRLFVTTDRPEEAASALRRVFGIASLSRVEECTSDIREVRALGLRMATPLLKDGA
                     SFRVEARRNGVHPYTSMQLAAEVGEEIYEANRHRGVRVDLHSPDQVVYVEVRDRRAYV
                     FTEYLEGPGGLPMGSQGRVVAVIEGERDALAAWMMMKRGCRVLYLGDEEGAMRAIERW
                     DPEPRTVSGPLERAVHAHRALGVVVGWSLQELQSRRTLNVDVPVYHPLIGMGEEEVEG
                     RLKMIVE"
     gene            10379..11221
                     /locus_tag="PLJ11_01270"
     CDS             10379..11221
                     /locus_tag="PLJ11_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOO03335.1"
                     /translation="MEPLVLLLIALLCVGAGIIGALLGIGGGLIIIPLLTLGFGVPMQ
                     EAIGASLIGVIATSTGAASRYVKQGLTNVRLALFLEMATTIGSIIGALIAVYTDQTVL
                     LVLFSLLLVYSGYTMLRRTEVILSPEEAPEGQVVDLSCRYIDPRDRCEVSYGVGGLGK
                     GLAFSSVAGMLSGLLGVGGGLVKVPVMNLCMGVPLRAATATSNFMIGVTALTGAMVFY
                     GHGLLNAYLCGAVALGTFTGAYIGTILMARYSPSALRRWFSILLFTVAFAMMLKALGL
                     WGVV"
     gene            11206..11559
                     /locus_tag="PLJ11_01275"
     CDS             11206..11559
                     /locus_tag="PLJ11_01275"
                     /inference="COORDINATES: protein motif:HMM:NF019459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HOO03336.1"
                     /translation="MGGGLTPDQDRLCSVVQPILRWGMVLSLCLMLLGLVLGLATGEG
                     EYAAVPMGELPSRLAELDPTAFLTLGIVMLIATPLARVLGSLYVFIKEGDGRFVLVSL
                     VVLFTVALSVLLGAA"
     gene            11619..12950
                     /locus_tag="PLJ11_01280"
     CDS             11619..12950
                     /locus_tag="PLJ11_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO03337.1"
                     /translation="MAKERSKDDRWYYSFLPYNISGGSTNALIPLFITEGLKGTVAQV
                     GIVSAATSLASVPAGIIWGNLSDTSKRRIPFVLAGFLGVAFSFILMGLSQSITEYYIA
                     NFIFGFLGAAVAPVGTVMVLECFHRDEWPRRLGDFSRVGGVGWVIGLLLGTVWLALFP
                     SEGDATPMRALFILAGALGFLSVFLGRLYMKEPVEKLSREAVSPMELNKVPSYLMEKL
                     RFMPQRLLYVLEMSRRNLRLRNFPRLLRRYYLIVFLAFTGFLCFYVVLPTYLSQYVGM
                     SSAQVLAVYLASSAVSALLYSRAGRLVDRFGGRKVQVLSFGLRALIFPSFYLVTLMPV
                     GQGALFVLMCALSGMAGLCWALLAVAGDSLVAKMAFRSYRSQSMGMYSSVRGIATLVG
                     SLLGGLVAQFLGYVPLFLLSSFFIVIAIAILSFTNVDEGSDEAAQPSHGSA"
     gene            complement(12942..13382)
                     /locus_tag="PLJ11_01285"
     CDS             complement(12942..13382)
                     /locus_tag="PLJ11_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO03338.1"
                     /translation="MRLLVIYDTVYGSTERIARAVAEELEAAGHEVSCRRQSLSGEED
                     FLGVGVWVIGSPTHWGRAPFRFKTLLKNAVKEAGADHAFVAFDTRYDGMHAGAADRTH
                     RMLVNAGLRPLLPPASFIVEGKELRAGEEARARALGQAIAASLR"
     gene            13496..14566
                     /locus_tag="PLJ11_01290"
     CDS             13496..14566
                     /locus_tag="PLJ11_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOO03339.1"
                     /translation="MRNSCDQGRGWTTGKMGIGTKLRLGISIGLIVLVILASNPEEVW
                     TALTGINVNLILLVVVLYLINLFVKAYRWSVLMGCAGNSVPFRTTFTAFSLCQAINNL
                     IPGKVLGETSRIVEMSAREGVAIGKGLASVVTERIMDFAVLTILAVTSLFLLLASIVE
                     EQRSYLILLVALMVVANVLLIYVLIRPKLLMRIGAWGARLTSKAIKGEKGERYAAKVL
                     GTVRSFNEAVAFSDKRLLVWPSMLTLLIWGNEIARLYLIIEALGVDVTLVAVIASASL
                     ASLSAVVLTAGSGNVLMSSAVLSASGLDPHVAATAGLLSAITSIWLSVPVSLLAMVVD
                     HRVQQAQSEDLKDADKNQRGYN"
     gene            14578..15120
                     /locus_tag="PLJ11_01295"
     CDS             14578..15120
                     /locus_tag="PLJ11_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO03340.1"
                     /translation="MLLLKNEEGNVGPLTEDILAVYRDQGIDGEVLLVDDGSTDRSGE
                     ICDQLAKDHPNVRVVHHEKNLGRSYAIRTGFQQAKGDVVIIMDADRQYEPKQIPLFLA
                     KIDEGYDIVTGWRKERTDPFVRRLISRTYNRFIIRRKFGLEIKDQNSGFKAFRREKAL
                     ARIDRARAYIESLGVREEQS"
BASE COUNT         2872 a         4813 c         4932 g         2596 t
ORIGIN      
        1 gaaatcgccc ttcaggaggt actccgtgtc ctcctcgtcg aactcggtgc aatgcaccat
       61 ccagttgcgg acctgcgcat tcacagggca aaccatctgg cagatcatgc agccgattac
      121 ggcgttgtgc atcccctcca ttatgtaatc gggaaagccc cgctcgttcg gcatctcgtt
      181 gtggaacgtg aggcacctct ccccgaacat gaggaagcgg tccctcacga tggcagcggt
      241 aggacaggca tcgacacagg ccatgcagcc ctcgcacctc tccatcatcc gcatctcctg
      301 ccaatcatca ggaagttcgc gatccgtgaa gaagctggta agcctgtggt acgaaccgta
      361 cttcgggaca taggtgatgt tgttgcgtcc gtacgccgcc agccctgtcc tggccgccag
      421 cgtcttgtgc ggaagaatgg cccgcatgag cctcactccc ctaccgtccc ccttctccag
      481 aaggtccacg atctccatgt ccacctccac cgcgttcgcg taggtcgggg ggacgatggc
      541 cctccgcctt ctccccccga ccctgaagac gacctcgcac gtcggctggg ggacggagac
      601 gacgacgatg ctccgcgcct ccgggaaggc ggaagggacg tagtacttga agcggaatag
      661 gtactccttg tacagctcct cgcttatgag cccctcccga tgaagggatt ccacgtcctc
      721 cctgacctcg tccagatggg acacggacag ggacctggca tcataccctc gctcacggag
      781 ggggcctaac atgctgcctt cctcggacat cggggccttc tgctagggaa tcctgcttcg
      841 ggattaagca ctttcccctg ttaacacgcg atgatttatc taggcggaaa cggtaggccg
      901 ggcgaccatg aaggaagtgc tagatagggc gctcagggtg caggacatcg atctctgcga
      961 ccattgcctg ggccggctgt tcgcccaggt gggcaccggg accacgaatc tcaacagagg
     1021 gcaggagctg cgggcgctga tcgaccagga ccgcgcctcc cggggcttgg ggccgatgag
     1081 gcacaccaga tgctcggtct gcgccgagct gttcgatcag gtgccacggt tcgcgggggc
     1141 ggtcgccctt aagttgcagg aagtggagtg cgataacttc ctggtcggaa ctagggtcga
     1201 cccgatcgtg caggcccggg aggaggggct gtggtccatc gtcggccagg agcacgccga
     1261 gcccatcaag tccgagctca accgggagat agggaaggcg gtgagcgccc tcaatggcaa
     1321 ggaggtggag ttcaagagcc cccaggtcgt cgccctagtg gacacccgct tcgcccatgt
     1381 ggagctggac gtggccccgc tgttcatcta cggccggtac cgcaagctct cccgggagat
     1441 cccccagacc aaatggccct gccgggcctg ccagggaaag ggctgccccc ggtgcgccaa
     1501 cacgggcaag atgtacgcta ccagcgtcca ggagcagata ggggacgtgc ttctccccta
     1561 cgccaaggcg aaggggcact tcttccatgg gatgggccgg gaggacatcg acgccctgat
     1621 gctcgggacc ggtcggccat tcgtcatcga gctctcctcc ccggtcatca ggcacctcga
     1681 cctgcccgcc ctggagcggg agatcaacga gagggggaag ggcctggtgg aggtggaggg
     1741 gcttcgcccc tcggaccgcg aggaggtccg gagggtcaag aacgcgaccc ctgagaagga
     1801 gtaccgcgca caggtggcca tccacggcaa agttaataag gagagagtgg atgaggtcct
     1861 tcgcacgttc aaccgaaccc gtatagtcca gcggacgccc gttagggtgg cccacaggcg
     1921 cgcggacctg gagcgtgagc ggcacatcgt ctacgtggtc ctggaggact tccaaggcga
     1981 ccggatgacg ctccggctgc gcaccgagtc tggcacctat gtgaaggagt tcgtgcacgg
     2041 cgaccagggg aggacgcagc ccaatctctc ggagatgctg ggggtcccct gcaccgtgga
     2101 ggccctggac gtcatcgggg tcatagacag cagcgaggga tagaaatggt caaagcatcg
     2161 catggattga ggaggaaggg aaggaaggtt ctaaagaaga gccccagggc caggggtctc
     2221 tccccgatca cccacgagtt cgttcagttc gaggtagggg agaaggcgca catccacctg
     2281 gaccccagca tcgtcagggg cgccccccac gtccgcttcc acggcaagac cgggacggtg
     2341 atggccgccc aggggcgcgc ctacgttctc tccgtcaagg acgggaataa gctcaagacc
     2401 gtcatctccc ggcccgagca catgcgcaag gtcaagaact gacggtgatg cggatgagca
     2461 aggatcgcta cgtcaccctg gcagaggtca aggagatgct cgaggccgag tccaaggcga
     2521 gggagttcac ccccgagcag aagctggcct tggagcacgc caaggacacc aagcttagcg
     2581 ccaaggaggc ccgggagcta gtcgaaaagc tgctggagct cgagttcatt gccgacctca
     2641 acaacgccga ggccgtggcg gtcaagatca ccgacctcct gcccacccac ttcgacgacg
     2701 tccgcctgat attccagaag gagaggcggg tgctcgacaa gaagcagggc gagcagatta
     2761 ttaaactggt gaacgaatac ctttaggctg gtgttgcttt ggaggactac gctcatattc
     2821 tagattatct accgcaggga ctgcctacgg agaggggatt caggagggaa ccggtagcgt
     2881 acgccctggg cagcaccgag ctcaagctgt tcgagctcgt gcccaagccg ggggcgatca
     2941 tcacccccgg ggaggtggtg tacatcggca aggacatgga caagcgcgat aagatattgc
     3001 acgtcaagcg ccgggtcggg tacgaggagc tgaacgcggg ctcccagaac gagctcccgt
     3061 tcgtgatcct ggaggtggtg aaggagaacg agccgcggtt cgtgcagttc ttcaacgagg
     3121 ccgagtcgat caccacccgg taccacgtgc ttgagctcct tcccggcctt ggaaagaaga
     3181 ccatgtgggc catcctcgag gagcgcaaga aggccccctt caaatcgttc gccgacatcg
     3241 ccgcccggat cccgaccttc aagcacccgg agaaggtggt ggcgaagcga atagaggtcg
     3301 agctggccga tccctcgcag aagtaccggg tgttcgttcc caggtaagat gatgagcatc
     3361 ggggagctgc gctcccttct agcaaggtac ggcgtccgcc cctccaaggc caaggggcag
     3421 agctacctca tcgacaggcg ggtggcggag cgggaggcgg aattcctcgg catagccccg
     3481 ggggaccggg tcctggaggt cgggccaggc ttcgggaccc tcacccgcgc cctcctggat
     3541 aggaccgaca tggtcacagc catcgagctc gaggagggcg cggtgcgcta tctccgggaa
     3601 accttccccc ggcttgaggt catccagggg gacttcctgg ccatagatgt cccccggttt
     3661 gacctcttca tctcgaacgt cccctacagc atatcatccc cgctgctgtt ccgcctcctg
     3721 gagatggact tcagaagggc ggtcctgatg gtgcagcggg agttcgccga acgcatggtc
     3781 gcctccgccg gggatgacgc gtattcccgc ctttccgtaa acgtctacta ccgggccgaa
     3841 tgcaggctcc tggagaatgt cccgaggtcc cgcttctggc ccgaaccgga ggtggattcc
     3901 gcgatcgtca tgctcacccc ccgccccgcc cccttcaggg tgagggacga gcggcgctac
     3961 cttgaacttg tcgatacgct cttccagcag aggcggaaga agatcgggac ggtgctcagg
     4021 cagaggggca tgctgcccga cgatcggagc aacgttccct acctggacga aagggtcgag
     4081 cggctcgctc ccgctcagat cgcggagctc tccgacgccc tcaccggtca ttgaacgagg
     4141 gctgaagcgg acccgggggc gcatcttgat tatcccgccg gccgatgtcg tcaggaggaa
     4201 catgtcgagc gccgagggaa cgatggacat agccgagatg ggaggaacgc tcaagagggc
     4261 agggcatctg aggctcgagc ccctgtgcat ctatgagagc aaggaaaggc cggagggggg
     4321 caggcccttc ggggacgtgg acaggtgcat cgccaaggcg gtgctgatgt gcgccaaggg
     4381 ggaggagccc ctgctctata ttagcgcgga cgcccgcgcg ggggtatgcc ccggcgggca
     4441 gtcctggctc ggcctgaccg agatggccga ggggctcaag tacttcatat ccaccgggtc
     4501 cccggccttc aggggagggg aggcggaaca tttgaagcgg gaccccgagc tggtgacgcg
     4561 gagcaaggcg ttcgtcggca gggtccgccc tccgcaggcc catctgtgcg tcgctcaatg
     4621 cgccaggtac cggcagggga cgggccagcc gagggcaatc ctcctgttcg cggaggccga
     4681 gcagctcagg aacctgctgg cgctgcacca tttcggcacg ccggacgcgt tcacctccac
     4741 ctccgcgccc tggggcccga gctgcgcctc cttcctgacc tacccggccg gcctggcctc
     4801 caactgcccg gaccaagccc tcatcatcgg cccggtggac cccaccggga accgatggct
     4861 gccgccggac ctgatgtccc tgggcatcaa ggtcagggac gccgagcgga tggcgaggga
     4921 cgtgccggcg tcgttcctga ccaagcgccc gcaggtcgcc ttcccggatg ggcgatgacc
     4981 gtcccccagg ccccggcggg caatcatttt aaccccggcc tccttatgga gaggaggaga
     5041 gcatgctgat cgggatcgta gggaagccca acgtgggcaa gtccacattc ttcgcggcgg
     5101 ccacgatggc cacggccgag atcgcaaact atccgttcac caccatcaag cccaacaagg
     5161 gcgtgggctt cgtacgttcc aggtgccctc acctggactt caatgtcaaa tgccagccca
     5221 ggaactccgc ctgcgagaac ggggtccggc tggtgccaat cgagctcctg gacgtggccg
     5281 gcctcgtccc cgacgcccat ctgggcaaag ggctgggcaa ccagttcctg gacgacctga
     5341 ggcaggccga cgccttcatc cacatcatcg acgccagcgg ctccacggac gcggagggca
     5401 acgccgtgag gccgggaagc cacgatccga tggaggacgt catcttcctt gagcgggaga
     5461 tcaatcagtg gataaagggc atcatgatca aagggttcga caagctggcc cgcggggccc
     5521 acctggaggg gcagaaggtg gagcaggtcg tccatgacag gctcacaggc ctgggggtga
     5581 ccgaggccca ggtctccgcc gccctgcgcc tttcggacat gccccagagg atcatcgatt
     5641 ggcaggacga ggacctctat cgcctggctg ccaatatcag acggttgagc aagcccatgg
     5701 tcctcgccct caacaaggcg gacgcggccg atcccgaaac gatcaggagg ctgagcgagc
     5761 tggaagggta tgtggctgtg cccacatgcg ccgagaccga gctggcgctc cggcgggcgg
     5821 ccaaggcggg ccttgtgtcc tataccccag gggacccttc gttcaaggtc aacgacccct
     5881 cgaagctgag cgccgggcag aggaaggcgc tcgactacat ggctcgcgcg atggaaaggt
     5941 atgggggcac cggagtccag agatgcctgg aaaaggtggc ctatgagctg ctggagatgc
     6001 tggtcgtgta ccccgtcgag gacgagggcc atctcaccga ccatgacggg catgtgctcc
     6061 cggacgcctt cctgctgagg agagggagca ccgcccggga cctcgcctac aaggtgcaca
     6121 ccgacctggg ggacaatttc atacgggcca tagacgcccg gacccatcgg gtggtcgggc
     6181 acgaccatcc gctgaaggac ggggacgtca tgacgatagt ggccaagagg taggacatgg
     6241 gagaatggga cgttcgcctg ctcacagcct cctattcgag gggggagaac gacaacatag
     6301 tggtggagct gttcgggaag acccgcgacg gccgctccat caccctgcgc cggacggggt
     6361 tcagacccta cttccacgtg gcggccccgc ccgaggaggt gcttccccac gtgcagggga
     6421 aggacgacct cctggccgtg gagcccgtgg acctgttcct gatcaacaag ggccgcgtcc
     6481 agcggtgctc caaggtggtc atcaggttcc cctgggccgt gcctcagtac cgcgccgagc
     6541 tgaagcgcca gggcatggag gtctatgcgg ccgacatccc gttccaccac cgcttcatct
     6601 atgacaacga cctcccggcc tgcttcaccg cccggggcga ggaggtcaag gataggtaca
     6661 cgaccgacct ggtggtgaac cttcaggggt tcaccgaggt cccccccttc acccccaagc
     6721 tcaagatcct gagcttcgac atcgagaact ccatcacgga cgagacgatc tacaccatct
     6781 gctacgtcat caaggacgag gccgggacgc gggcggggga gcccatcact ggcggcgagc
     6841 gggagatgat cgagcggttc accgaggtca tccaaatgga ggacccggac gtcatcaccg
     6901 gctacaacat cgacgggtac gacatcccct acataatcga aagggcgagg aagctgggcc
     6961 tcaacgagct caagtggggc agggacgggt cctctcccag acgggtcatg aaccgcttct
     7021 ggcgctgcac cggccggctc atcgtggacg cctggtgggc ggtgaaggtc gagataaggc
     7081 cgaagcagga gacgctgaac gccgtctcca aggccctgct gggcgaggag aagctggacg
     7141 tcgacatgaa gatgaaggtc ggggatgact acgacccgag ggcgatagac aaggaatgga
     7201 cgaacgaccg ggacaaggtc ctggcctact gcacccggga tgccgagctg gcgctcaagg
     7261 tcctgggcaa ggtggggcgc atccgcaagg gcatggacct ggccgctgtg tcccgcatgc
     7321 ccgtggacga tgtcctgaac tcgggctcat cccagctgat cgacgccata atgatccgcg
     7381 cggcggaccg ccacgtccca caggtggcgg tgccgctcac aggcagcttc gaagacgagg
     7441 aggaggccat cgaagggggc tatgtgcatg agatagcccc cggcgtgtac cattgggtgt
     7501 gcgtcctgga cttcaagtcc atgtacccca gcctcataat agccaagaac atctgcttca
     7561 ccacccggga cgaccgcggg acgatcgtat ccccgctcgg ggtccgcttc ctgagcaagg
     7621 agcagcagcc tggcatcctc ccgacgatat tggcggacct catgcgccgc agggacgaga
     7681 cgaaggcaat ggcgaaaagg gcgaaggacc ccgaggaggc ccattactac gagggcctgc
     7741 aggcggccat caaggtcctc atgaactcct tctacggcgt cttcgcatcc tccttctacc
     7801 ggttcaccga ccgcagcata gggggcagca tcacggcctt cgccagggag accaccaagg
     7861 gcattatcaa ggccctcacc gacgaggggg tgaacgtcat ctactccgat acggactcgg
     7921 tgttcgtgca gtccccccat caggacctcg aggggtcgct ggccttcggc cgggagctgg
     7981 ccgcccgctt ctccaaggag gggggctccc tggagttcga gaagctcatg gagccgctgt
     8041 tctcccacgg caagaagaag cgctacgtcg gccgtatggt ctggccgaag gaggagctgg
     8101 tcatccgcgg gtacgaggtg cgcaggacgg acgccttcga cctgcagtcc gaggcgctga
     8161 tgaccgtctt cgagttcata atggacggga agacggagga ggcggtgaag gccgcccgag
     8221 ggtacgtgca ggacacgctc gcggggaaag cgccgctgga gaagctggtc atatcgaagg
     8281 ggtgccgggc cttcaaggac tatgccaacc cggacaccca ggccaccgtg cagaccgccc
     8341 ggaagctcat cgacatgggg tacgagttcg tcccggggat gaaggtctcc tggatcgtca
     8401 ccaactcgcg gaagaggccg caggaggtcc agccctacat ctcagggaag gagttcggct
     8461 acacccccga ctaccggtac tacgccgagc ggctggctca gacgcttgca cgcgcgaccg
     8521 aggtcttcgg ctggggggaa aaggacctca tgaacggcaa ccagcaggcc agcctgttcg
     8581 actatggctc ggaggagggg cagaggccga aggtgaggct ggagcaggtc aagaagaccg
     8641 ataggaagct caccctggac gattacttct gaggcggccg ggaaatcgtt taaatagggc
     8701 tatattatct gtgggcctcg tagcgagacg agtctcgccg gagctgtacc ccgcaagggg
     8761 cgaatcccga agcgccgcca tctgcggcac tggtgataca aatggtggac aacaagaccg
     8821 acgatagcgg ggaagggcca aggccagtga gcggaaggag catgtactcc tacatctcgg
     8881 aggcctggga ccgtcccgac gactcttacg tgaacgaact tatgtgggaa aggctcatcc
     8941 tgtggaggag ggagccgaac ttcagccggc tggagcggcc gacgaggctc gaccgcgccc
     9001 gcgccctcgg gtacaaggcc aagcagggca ttatcgtggt ccgcggccgc gtccggaagg
     9061 gcgggctgca gaggcggaag atctggaagg ggcgcagggc caagcgcaag ggcatgacca
     9121 agatcaccac gggcaagagc ctcaagagga tggccgagga aagggcggcg aagcgctatc
     9181 ccaacatgga ggtgctgaac tcctattggg tgggggagga cggcaagaac gtctggtacg
     9241 agatcatcct ggtcgacaag caccatccct cgatcattgc ggacaaggac ctcaactgga
     9301 tctgcgggag cgcccacaag aacagggtgt tccgcggcaa gacctccgcc ggccgcaagg
     9361 ggcgcggcct gcactggaag ggcaaagggg cggagaaggc gcgcccgtcg gtgcgcgcca
     9421 atgaccacca cataaagtag gcgccccgcc tactcaacta tcattttcaa ccgcccttcg
     9481 acctcttcct cccccatacc gatgagggga tggtatacag gcacatcgac gttcaacgtt
     9541 cggcgggatt ggagctcctg gagcgaccag cccacaacga cgccgagggc ccggtgggcg
     9601 tgcaccgccc tctccaatgg cccggaaacc gtcctcggct cagggtccca gcgctcgatg
     9661 gcgcgcatcg ccccctcctc gtcgcccaga tagaggacgc ggcacccccg cttcatcatc
     9721 atccaggcgg ccagcgcgtc ccgctcgccc tcgatgacgg ccacgaccct cccctggctt
     9781 cccatgggaa gcccgcctgg cccttccagg tactcggtga agacgtacgc ccgccggtcg
     9841 cggacctcca cgtacaccac ctgatcgggg ctgtgaaggt ccacacgcac ccctcgatgc
     9901 cggttcgcct cgtagatctc ctcccccacc tccgcggcca gttgcatgct cgtgtaggga
     9961 tggacgccat tgcgcctggc ctccacccgg aaggaggcac cgtccttgag aaggggggtg
    10021 gccatcctga gccccagggc gcgcacctcc ctgatgtccg aggtgcactc ctcgacgcgg
    10081 ctgagcgatg cgatcccgaa gacccgcctc agcgccgagg cggcctcctc cgggcggtcg
    10141 gtcgtcacga agagccggcc atactggcct tcgatgaggg cctccaccct ctccttggcc
    10201 agggcggcca tcatgttgcg aatgagcacc ttctcgaaat agcgccgcac ccccggcccc
    10261 ttcagcccta gctccgagta tctgaccagc aggaggccca tgcgccttcc atggagcggg
    10321 aggtaataaa ccattctccc gctagcctta tgtccctctc cgggcatggc ctgcatcaat
    10381 ggaaccgctg gtcctgctgc tcatcgccct cctctgcgtg ggcgccggca tcatcggagc
    10441 cctgctgggg atcggcggcg ggctcataat aatccccctg ctcaccctcg gcttcggggt
    10501 ccccatgcag gaggccatag gggccagcct gatcggtgtc atcgccacct ccaccggggc
    10561 ggccagccgg tacgtcaagc aggggctgac caacgtccgg ctggcgctct tcctggagat
    10621 ggcgaccacc atcggctcga tcatcggagc gctcatcgcc gtgtacaccg accagaccgt
    10681 cctcctggtc ctcttctccc tgctgctggt gtactccgga tacacgatgc tccgccgcac
    10741 agaggtgatc ctctccccgg aggaggcgcc ggaagggcag gtggtcgacc tgtcctgccg
    10801 gtacatcgat cccagggacc ggtgcgaggt cagctacggc gtcgggggat tggggaaggg
    10861 gcttgcgttc agcagcgtcg ccggcatgct ctccgggctt ctgggcgtgg gcggcggact
    10921 ggtcaaggtg ccggtcatga acctgtgcat gggcgtcccg ctgcgggcgg ccaccgccac
    10981 aagcaacttc atgatcggcg tcacggcgct cactggggcc atggtcttct acggccacgg
    11041 ccttctgaac gcctatctat gtggagcggt ggccttgggg accttcacgg gcgcttacat
    11101 tgggaccatc ctcatggccc gttactcccc cagcgccctc cgccgatggt tctccatcct
    11161 gctttttacc gtggccttcg ccatgatgct caaggccctc ggcctatggg gggtggtctg
    11221 acgccggacc aggacaggct ctgcagcgtg gtccagccga tcctgcgctg gggcatggtg
    11281 ctcagccttt gcctcatgct gctcggcctg gtcctggggc tggcaaccgg ggagggggag
    11341 tacgccgcgg tccccatggg ggagctcccc tcacggctcg ccgagctgga cccgaccgcc
    11401 ttcctcaccc tgggcatagt gatgctgata gccacccccc tggcaagggt gctcggttcg
    11461 ctgtacgtct tcatcaagga aggggacggc aggttcgtcc tggtatcgct tgtggtcctg
    11521 ttcaccgttg ccctctcagt cctcctcggg gccgcctgag catcgacgtc cgtggaaccg
    11581 cagggtttat ccataggggc caacaatcct gaggggacat ggccaaggaa cggtcgaagg
    11641 acgatcgatg gtactattcg ttcctcccgt acaacatatc gggggggagc accaacgccc
    11701 tgatcccgct gttcatcacg gaggggctaa agggcacggt ggcccaggtg ggcatagtca
    11761 gcgcagcgac ctccttggcg tcggtcccgg ccgggatcat ctgggggaac ctctcggaca
    11821 cctccaagcg aaggatcccc ttcgtcctgg cggggttcct cggcgtggcg ttctccttca
    11881 tcctgatggg gctgtcgcag agcataacgg agtactacat cgccaacttc atcttcggct
    11941 tcctgggtgc ggccgtcgcc ccggtcggca ccgtcatggt gctggaatgc ttccacaggg
    12001 acgaatggcc gaggcgattg ggggatttca gccgggtcgg aggggtgggg tgggtcatcg
    12061 ggctcctgct gggcacggtc tggttggcgc tgttcccatc cgagggggat gccacgccca
    12121 tgcgcgccct cttcatcctg gccggcgccc tgggcttcct gtccgtgttc cttgggcgcc
    12181 tctacatgaa ggagccggtg gagaagctca gcagggaggc ggtctccccg atggagctga
    12241 acaaggtccc ctcctacctg atggagaagt tgcgcttcat gccccagcgg ctgctctatg
    12301 tgctggagat gtccaggaga aacctcaggc tgcgcaactt cccacggctc ctccgccgct
    12361 actacctcat cgtgttcctc gccttcaccg gcttcctgtg cttctacgtg gtcctgccca
    12421 catacctatc gcagtacgtg ggcatgagca gcgcccaggt cctcgcggtc tatctggcga
    12481 gctcagcggt atcggcgctg ctctactcca gggcgggaag gctagtcgat aggttcgggg
    12541 gaaggaaggt ccaggtactg tcgttcggac tgcgcgccct gatcttcccc tctttctacc
    12601 tcgtcaccct gatgcccgtc ggacaggggg cgctgttcgt cctgatgtgc gcattgagcg
    12661 gaatggccgg gctgtgctgg gccctcctgg cggtggccgg ggactccctt gtggccaaga
    12721 tggccttccg atcctaccgg agccaatcca tgggcatgta ttcctcagtg cgcggcatag
    12781 ccaccctggt cgggtcgctc ctgggcggcc tggtcgccca gttcctcggg tacgttccgt
    12841 tgttcctgct ctcctccttt ttcatcgtaa tcgcgatcgc tatcctgtcc ttcaccaacg
    12901 tggatgaggg gtcagacgag gcggcgcagc cttcgcatgg atcagcgtag cgaggccgcg
    12961 atcgcctggc caagggctct cgccctggcc tcctccccgg cgcgcagctc cttcccctcc
    13021 acgatgaacg aggcgggggg gagcagcggg cgaagtccag cattgaccag catcctgtgc
    13081 gtgcgatcgg ccgccccggc gtgcatgcca tcataccggg tatcgaaggc cacgaaggcg
    13141 tggtccgctc ccgcctcctt gacggcgttc ttgagcaggg tcttgaagcg gaagggcgcc
    13201 cgcccccaat gggtggggga gccgatcacc caaaccccga ctccgaggaa gtcctcctcc
    13261 cccgagaggg actgccggcg gcaggagacc tcatgcccag cggcctcaag ctcctcggcc
    13321 accgcccggg ctatccgctc ggtgctgccg tacacggtat catagatgac cagaaggcgc
    13381 atcctatcgt tcccgtgatg gaccggaggg attacattct ttgcgcatcc ggcccaggcc
    13441 gatggcaaag gtagatataa gtccatccta gtcatcagat gggccaacgg cctctgtgcg
    13501 gaacagttgc gatcagggaa ggggatggac cacgggcaag atgggcatcg gcaccaagct
    13561 caggctgggc atttcgatcg gtctgatcgt gcttgtcatc ctggcatcca accccgagga
    13621 ggtctggaca gccttgacgg gcataaatgt aaacctcatc ctgctggtcg tcgtcctcta
    13681 cctcatcaac ctcttcgtca aggcctaccg ctggagcgtg ctgatggggt gcgcagggaa
    13741 cagcgtgccc ttccgcacca cattcaccgc cttctccctt tgccaggcca tcaacaacct
    13801 catccccggc aaggtgctcg gcgagacctc ccggatcgtc gagatgagcg cccgggaggg
    13861 ggtggccatc gggaagggat tggcgtcggt ggtcaccgag cggatcatgg acttcgcggt
    13921 gctgaccata ttggccgtca ccagcctgtt cctgctcctc gcctccatcg tggaggagca
    13981 gcgcagctac ctgatacttc tggtggccct gatggtcgtg gcgaacgtcc tgctcatcta
    14041 cgtgctcatc cgccccaagc tcctgatgcg catcggggcc tggggggccc gtctgacctc
    14101 taaggccatc aagggtgaga agggcgagcg gtacgcggcc aaggtcctcg gcacggtgcg
    14161 ctcgttcaac gaggccgtgg cgttcagcga taagaggctg ctcgtctggc catccatgct
    14221 caccctactg atatggggca atgagatcgc ccgcctgtac ctgatcatcg aggccttggg
    14281 cgtcgacgtg accctggtgg cggtcatcgc ctccgcaagc ctggcatcgc tgagcgcggt
    14341 ggtcctcacc gcgggcagcg gcaacgtcct gatgtcctcc gcggtgctga gcgccagcgg
    14401 cctggacccg cacgtggccg ccaccgcagg gcttttaagc gcgatcacct ctatctggct
    14461 gtccgtaccg gtcagcctgc tcgccatggt ggtggaccat cgcgtccaac aggcccagag
    14521 cgaggacctg aaggacgccg acaagaacca gaggggatac aactgaagtg caccaccgtg
    14581 ctgctgctca agaacgagga agggaacgtg gggccgctca ccgaggacat cctggccgtc
    14641 tatcgggacc agggcatcga cggggaggtg ctgctggtgg acgacgggag caccgatcgc
    14701 tccggggaga tatgcgacca gctggccaag gaccatccca acgtgcgggt cgttcatcat
    14761 gaaaagaacc tggggcgctc ctacgccatc cgcaccggct tccagcaggc caagggcgac
    14821 gtggtgatca tcatggacgc cgaccgccag tacgagccca agcagatccc gctcttcctc
    14881 gccaagatag atgagggata tgatatcgtc accggctgga ggaaggagcg caccgatccc
    14941 ttcgtccgcc gactgatctc ccgcacctac aaccgcttca tcatccgccg caagttcgga
    15001 ctggagatca aggaccagaa cagcggcttc aaggcgttcc gcagggagaa ggcgctggcc
    15061 cgcatcgatc gggctcgtgc ctacatcgaa agtctgggcg tgagggagga gcagtcgtga
    15121 gtgagaagtt cctggtggga gtggacctcg ggggaaccaa cgtacgggcg gccgtggtga
    15181 atgcattaac gagcgcggtt aacgatttcc aag
//
LOCUS       DAOSYN010000014        14162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_3616_length_14162_cov_3.740625, whole genome shotgun sequence.
ACCESSION   DAOSYN010000014 DAOSYN010000000
VERSION     DAOSYN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 14162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14162
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3616_length_14162_cov_3.740625"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLJ11_01300"
     CDS             complement(<1..226)
                     /locus_tag="PLJ11_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HOO03341.1"
                     /translation="MDRETIAKVAKVARLALTDEEMERYAQDLDAILNSFAVLDEAPA
                     SDTYALNPVPVVDVLREDEPASIRWWALWCS"
     gene            334..762
                     /locus_tag="PLJ11_01305"
     CDS             334..762
                     /locus_tag="PLJ11_01305"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOO03342.1"
                     /translation="MRLRLYTDGGSRGNPGASAYAYIICDEEGREIASGSNYLGVMTN
                     NEAEYHGLLAGLKEVQRRGATEVEVFMDSELAVKQLKGQYRVKAANLAPLHREAVAIL
                     AGMAHAKVAHVPREDALIAKADAMVNWTLDQHAMARRLRQ"
     gene            complement(904..2151)
                     /gene="hisS"
                     /locus_tag="PLJ11_01310"
     CDS             complement(904..2151)
                     /gene="hisS"
                     /locus_tag="PLJ11_01310"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOO03343.1"
                     /translation="MIQRPRGTRDFGPEEMEVRRHFEDMMRKEAALHGFREVATPIFE
                     HTELFTLKSGPGVVEEIYAFQDKGGREICLRPELTASVMRFFISDLTNLPRPLKLFYF
                     GQCFRYERPQAGRYREFFQFGAEIIGSPTPETDAEAIGLAAAILERTGLRDYRIRIGH
                     IGILREMLARAGVSGDAALPILQRLDKKEYPEAEALMTQAGMGREAIDGVIATTQVTG
                     PITVLDGVQGPAGAYIREVYDILQAMGFSNLSVDLGVVRGLAYYTGMVFEVDAPKLGA
                     EKQICGGGSYSLSELFGGEKVFSTGFAIGFDRTLLAIQAEGVEFVRPGITAYVVPVTD
                     SARMRAFQVVGMLRRAGVSADVDIMRRGVGKSLRYASSINAKYAVIVGEKELESDSVT
                     LRDMASGEQSLVRAEELPKRLKE"
     gene            2239..2607
                     /locus_tag="PLJ11_01315"
     CDS             2239..2607
                     /locus_tag="PLJ11_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03344.1"
                     /translation="MALDEEGQRRRARLLQAWADDLDRKGFVDIRVNLDRFPARPEGF
                     GEYVPDIVAMRKDGRRLVGEIWLCESLGSEGMLPRALSYSYRGDVVMLLVPFDCLDEA
                     EGLVREWGLESEVYVQGIID"
     gene            complement(2604..3773)
                     /locus_tag="PLJ11_01320"
     CDS             complement(2604..3773)
                     /locus_tag="PLJ11_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOO03345.1"
                     /translation="MSKKGMATRAVHDGDRSLRYEGAINTPIFQSSTFSFPTDDPRTW
                     EGEVPRGTYIYSRNSNPTVEAVEEKLASLEGAEASLAFSSGMAAISTALLAFVGKGDR
                     VVAMQDLYGGTYGLLRNEMPRLGVDVAFVPTTDPEELCAAIDGRTKVLYLESPTNPLL
                     KLIDIKETCRLAHEKGCRVLIDNTFATPVLQRPLEQGVDVVLHSATKYLNGHSDVIAG
                     FVAGRREDMGLVHQRRRLFGGVLDPLPAYLLARGMRTLDLRVRRHEANARAVAEHLEA
                     HPKVSRCIYPGLRSHPDHELAKRQMDGFGGMVTFEVRGGRHAAEGAIKRLQLIARASS
                     LGGVESLASMPLNTSHTSYSREERALLGITDGMVRLSVGIEDPEDLCQDLDQALR"
     gene            complement(3813..4928)
                     /locus_tag="PLJ11_01325"
     CDS             complement(3813..4928)
                     /locus_tag="PLJ11_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO03346.1"
                     /translation="MASTIEEQIKAIQEEIDKTQKNKATEYHIGKLKARMAKLKDDME
                     RRRASSGGSAKGYSVKKSGNATVALVGFPSVGKSTLLNTLTGAKSEVAAYDFTTLDVV
                     PGAYEYQGAKIQVLDMPGLIRDASKGKGRGREVISVVRSSDLILFVLDFYDTNLQVLV
                     RELENAGMRINAHPPDVVISQRETGGVEVKSTVKLTKLDLELAKAMVMEYGVVNADVV
                     IREDIDQDQLVDVLSGNRIYINAVLAINKIDLVDDEYLEEVRERFKDWDPVLISAAKN
                     IGIDELKAKIFERLELIRIYMKKQGQEADMDVPLIAKKGSTVGDVCDSLHRVFRQNFR
                     YALVWGDSARFPGQMVGLEHRLADGDVLSIIIRRTGE"
     gene            complement(4935..5909)
                     /locus_tag="PLJ11_01330"
     CDS             complement(4935..5909)
                     /locus_tag="PLJ11_01330"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOO03347.1"
                     /translation="MTGPGGGGGGLAVVALGGNAILRSGQEARGDVQIANMREVFRRT
                     LPLLDQYPHLVLTHGNGPQVGNILLRNELCRAEVPAMPLDVCVAESQGQIAYLALQAW
                     DQECLRAGREVPMLPMITRTVVAADDPSFLSPTKPIGPYYPKERAERLQWEKGWSFRQ
                     DPKRGGMRRVVPSPDVLRVAEEGHLKALCASLPPPFVVLCGGGGGVPVVQDGSGYRGV
                     EAVVDKDLTSARLAIILEADILIMVTDVEAVYLDFTSDRPRPLLRATASELRRHAAEG
                     QFPAGSMGPKAEAAVRFVEATGRRAVIASADRLMEALEGRAGTTVERG"
     gene            5955..6938
                     /locus_tag="PLJ11_01335"
     CDS             5955..6938
                     /locus_tag="PLJ11_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO03348.1"
                     /translation="MSFIDDINKMLKGVRQSKMSIWIGMGLTILVTVLLTLTIQLWLS
                     QVVCLLPILVALLAYYLPTYFGLKDRKKLALFGLVLFLVIGLSLGAALYKSVEGYEVV
                     ELSSDDDLLVQGTVSPSQGAPGDIFDFSVVLVSGNATADVRLLLYNNWGDPDDALNIS
                     MAFSHNVTLDDSSIGALYTTNTSSLEEGIYHFQYGALDGGAWTETEQGFGPVNAELGK
                     VLSNSLMSGVLYSFYSMAVLFYILILLTWWMDRSKKKMAEMEQQRAKVKEKKGAEEKF
                     VCSDCGAEVPRDADKCPACGAKFDDEEFKCPQCSAKLLRTDTKCWNCGKKL"
     gene            7017..9008
                     /locus_tag="PLJ11_01340"
     CDS             7017..9008
                     /locus_tag="PLJ11_01340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOO03349.1"
                     /translation="MGSIADELAKKQKEISVSEFFERNRHILGFDSQSKALIMGIKEA
                     VDNSLDACEEADVLPEIVVTVERTEKDEFRVSVEDNGPGIVKKAIPNVFGRLLYGSRF
                     HAVRQSRGQQGIGISATVMYGQITTGKPAVVISKTGADDVAWRMEIMVNTKRNLPDVL
                     KEDPFIWDGKEHGTKITYHMNGRYVGGRQSVLEYLRQTAIVNPHARITFTDPDGRRTT
                     FERATDRMPPATKEIKPHPSGLELGTLMSMAKETKNKSLGRFLQLDFSRISDRLAKDI
                     CERAGLDMMTDPTRLTLEEHKRLLAAIGTAKIMAPPTDCLSPIGAQLIKKGLRNVLDE
                     VRPEFYAPPITRDPKVHSGNPFLVEVGIVYGGDLPKDAPVQILRFANRVPLLYQQGAC
                     AITKAVESVDWRRYGLEQRGGSGIPFGPAIILVHVASTKVPFTSEAKEAIATIPEIQA
                     EIELALKICGRSLKTHLNKKETKSKTRVKFEIVQEILPLIAQKSAKIVGKPVPKLSGS
                     ITKIMNVVWVDDAVSFEKGHHRIRVSIYNYTPQSQKFNLHMVLPRGAFDYKGLQFFPT
                     EVREDGKVTWELPKIASTERLDINFSLSGLNKEDYDENEIYASGIDPVAIIGAEPLPG
                     DWDLKGLEVTESVEAKGPAPEEEEEEVDYDEAKEALNDD"
     gene            9001..10095
                     /locus_tag="PLJ11_01345"
     CDS             9001..10095
                     /locus_tag="PLJ11_01345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOO03350.1"
                     /translation="MTEEENRAVRSLKEIAGSIYDQIDSGKVPKMTLPLRTKSNIRFD
                     PKHGVFKYGKATGARSAKKVNGALMLLRTMYVLEFIEDMVQNGKSSTLREMYYISEGW
                     EKAKFSSQDESNMLAEDLEIVTACMREDFKLRAEENGASIIGDLTISEMDRKGKVKRI
                     NCRDDVGDSGYGIPNNVEREKVQLLGTNAKFVVAIETGGMFDRLVENGFDERYNAVLV
                     HLKGQPARSTRRFIKRLNEEMRLPVVVFTDGDPWSFRIFASVAYGAIKTAHISEYLAT
                     PTAEFVGITASDITNYDLPTDKLTDVDVRALNAELKDPRFADEYWRGEIETMLKLNKK
                     AEQQALAKYGLDFVTDRYLPEKLSNMGVLR"
     gene            10130..10504
                     /locus_tag="PLJ11_01350"
     CDS             10130..10504
                     /locus_tag="PLJ11_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03351.1"
                     /translation="MSGLKMNDHISITLTSLTASYIIIATAAAILIAWLTEDWTLFIP
                     SMLLLGGAFATYIGLKQRTRPLSRTERGNGNFLMFWGTFLIAISLIWAINYVYPGNGL
                     LLFIGLLVWLGIAVVLFTMKRG"
     gene            complement(10668..12200)
                     /gene="lysS"
                     /locus_tag="PLJ11_01355"
     CDS             complement(10668..12200)
                     /gene="lysS"
                     /locus_tag="PLJ11_01355"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOO03352.1"
                     /translation="MHWADVIANALVERGGKHLISTGITPSGYIHVGSLRESITANAV
                     YKALKERKADVDFIYLVDSYDPLRKRYPFLPERFEKEVGKPICYIPCPDGCHASYAEH
                     YITPFLDAIGELGIRPRIIWTHRLYEEGRFTEIVDLVLRERAKVAAILGEVTGRKMPD
                     GFWPFSAECPECGTFSDRIVSYEFPYVHFKCRCGYEGDADIRKGQGKMPWRVEWPAKW
                     KLFGVTCEPFGKDHAAAGGSYDSGVRLCREVYGAEPPYPVPYEFIQFKGRGQMHKSTG
                     NVVTGTDALLITPAPVLAFSVLRYNPERHIDYDPGMGILDIVDEYDKVEALYFNGGAD
                     EKERDLLRAYELAQPSGVRSRLPLQVPYRHLVSVAQIADTFEGAVAVLKRSMSIPEIS
                     EEDMEVLKQRLNCVRHWLNAFAPEEVLFTVSPERPDVELTEAERAYVQALRERLEGIE
                     WTGDRIHDAIYETAKAMGMQPKACFQALYRLFIARTSGPRLGFFLSTLDRGFVLKRLA
                     EA"
     gene            12322..12489
                     /locus_tag="PLJ11_01360"
     CDS             12322..12489
                     /locus_tag="PLJ11_01360"
                     /inference="COORDINATES: protein motif:HMM:NF002318.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HOO03353.1"
                     /translation="MAKENKTGGFQSAAGLIRYFDSEDEKALKLSPYVVIALCVITIA
                     LVVVASALYPT"
     gene            complement(12721..13989)
                     /locus_tag="PLJ11_01365"
     CDS             complement(12721..13989)
                     /locus_tag="PLJ11_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO03354.1"
                     /translation="MGEDFIQTVLFQLFVIIVLVEVARMLCNKVKVPTVIGEILVGVL
                     IANTFLYDFINLGSDREVFEVLKELGVIFLLFSVGLETPFSAIRKVGKTAMFVAVFGV
                     ILPFIVGYLLMIGALGPEKQLEALFVGASMVATSVGITARVIRDMNLMHTVESRVIIG
                     AAVIDDILGMIVLAIVVGIAGGGSLDIFQVAVVAIEAVLFVLAIIFIGSLVLPKVLKK
                     RDDRKVTQVCTAERRPKGISPLPLALIVCFALSSISSFLGLAAIIGAFLAGMAFAEFK
                     DRWPCEEKMEPIVEFLTPFFFVFVGIAVDLEAFGELGVLMMAATITVFAIITKFIGCG
                     LGARSLGGKSAAIVGVGMVPRGEVGLIVASIGLGMLDPETGLSIIEPGLYNAIVFMSL
                     VTTLVAPPLLTYTFQRKLGKKCEVLPPDVN"
BASE COUNT         2677 a         4466 c         4454 g         2565 t
ORIGIN      
        1 cgctgcacca cagggcccac cagcggatgc tggcgggctc gtcctcgcgc agcacgtcca
       61 ccactgggac cgggttgagg gcgtaggtgt cgctggcagg ggcttcgtca agcacggcga
      121 agctattcag gatggcgtcc aggtcctggg cgtatcgttc catctcctca tcggtgaggg
      181 cgagcctggc caccttggcc accttggcta tggtctccct gtccatgctt tgcacgtccc
      241 tgccttttta tgggcggagg aggttatact ttcgcggatt gtgaaggttt cgcatgccat
      301 ttggaataaa taggaaagga gatacgcctg ccgatgaggc tccgactgta tacggacgga
      361 gggtccagag ggaaccccgg cgcctccgcg tacgcatata tcatctgcga tgaggaggga
      421 agggagatcg cctccggctc caactacctg ggcgtgatga ccaacaacga ggcggagtac
      481 cacggcctgc tggctggatt gaaggaggtt cagcggagag gggcgacgga ggtggaggtc
      541 ttcatggact cggagctggc ggtcaagcag ctgaaggggc agtaccgggt caaggcggcg
      601 aacctcgccc ccctgcaccg ggaggcggtg gccatcctcg ctggcatggc gcatgccaag
      661 gtcgcccatg tcccccgcga ggacgcgctc atcgccaagg cggacgccat ggtcaactgg
      721 acgttggacc agcacgccat ggcccggcgg ctgaggcaat gaaagcatcg atataatatc
      781 gttgcagcaa gcaaatcatt atatatagga cgctaagtgc gccatctgag gctaaggggc
      841 cggaggcgtt ttcctcctac agcaattccc ttgtgatccc ccattcttcc ggccccgaag
      901 ccctcattcc ttcagcctct tcgggagttc ctcggccctt accaaggact gctccccgct
      961 ggccatgtcc cggagggtga ccgagtcgct ctccagctcc ttctccccaa cgatgaccgc
     1021 atacttggcg ttgatggacg aggcgtatct caggctcttc cccacccccc tgcgcatgat
     1081 gtccacgtcc gccgatacgc cggcgcggcg cagcatcccg accacctgga aggccctcat
     1141 cctggcgctg tcggtgacgg ggaccacgta ggcggtgatg cccgggcgta cgaactcaac
     1201 gccctcggcc tgaatggcca gaagggtgcg gtcgaagcca atcgcgaatc cagtggagaa
     1261 gaccttctct cccccgaaga gctccgagag cgagtaggag ccgccgccgc agatctgctt
     1321 ctccgccccc agcttgggcg cgtcgacctc gaagaccatg ccggtgtagt aggcgagccc
     1381 gcggaccacg cccaggtcca cgctcaggtt gctgaagccc atcgcttgga ggatatcata
     1441 cacctcccgg atgtacgcgc ccgcggggcc ctggacccca tccagcaccg tgattggccc
     1501 ggtgacctgc gtggtggcga tgaccccgtc gatggcctcc ctccccatcc ccgcctgggt
     1561 catcagcgcc tccgcctctg gatactcctt cttgtccagc ctctgcagga tgggcagcgc
     1621 cgcgtccccg gagacgccgg cacgggcgag catctccctc aggatgccga tgtgccctat
     1681 gcggatgcgg tagtccctca gccccgtgcg ctccaatatg gcggccgcca gccctatggc
     1741 ctcggcgtcc gtctccgggg tcgggctccc gatgatctcc gccccgaact ggaagaactc
     1801 gcggtagcgg cctgcctggg gcctctcgta ccggaagcac tgcccgaagt agaacagctt
     1861 cagcgggcgc gggaggttcg tgaggtcgct gatgaagaag cgcatcaccg aggcggtgag
     1921 ttctggcctc aggcagatct ccctgccccc cttgtcctgg aaggcgtaga tctcctccac
     1981 cacccccggg ccggatttga gggtgaacag ctcggtatgc tcgaagatgg gcgtggcgac
     2041 ctcccggaag ccgtgcagcg cggcctcctt tcgcatcatg tcctcgaagt ggcgccgcac
     2101 ctccatctcc tccgggccga aatccctggt cccccggggc cgttggatca tggggcttga
     2161 gatggagcgg ccgataataa acattgcgcg ccgggaccca gcataaaaaa gattagagga
     2221 acggggcata gcccccgcat ggccttggac gaggaggggc agcgccgcag ggcgaggctg
     2281 ctgcaggcat gggcggacga cctggaccgc aaggggttcg tcgacatccg tgtgaacctc
     2341 gatcggttcc ctgcccggcc ggaggggttc ggggagtatg tcccggacat agtggccatg
     2401 aggaaggacg gccgacggct ggtcggagag atatggctgt gcgagtccct gggctcggag
     2461 ggcatgctcc cccgggccct gagctactcc taccgcggcg atgtggtcat gctcctcgtg
     2521 cccttcgact gcctggatga ggcggagggg ctagtccgcg aatggggcct ggaatcggag
     2581 gtctatgtcc aggggatcat tgactagcgc agtgcctggt ccaggtcctg gcagaggtcc
     2641 tcggggtcct ctatgcccac ggacaggcgg accatgccat cggtgatccc gagcagggcc
     2701 cgctcctccc tgctgtacga ggtgtgggag gtgttcaacg gcatgctcgc caacgattcg
     2761 accccgccca ggctggaggc cctggcgatc agctggaggc gcttgatggc cccttcagcc
     2821 gcatggcggc cgcctcggac ctcgaaggtc accatcccgc cgaaaccgtc catctgcctc
     2881 ttggccagct catggtcggg atgggagcgc aggcccggat agatgcacct ggagaccttg
     2941 ggatgggcct ccaggtgctc ggccaccgcc cgcgcgttcg cctcatgcct gcgcactcgg
     3001 aggtccaagg tcctcattcc cctggccagt agatacgccg ggagcgggtc caggacgccc
     3061 ccgaagagcc tccgcctttg atgcaccagg cccatgtcct cccgccgtcc ggccacgaag
     3121 ccggcgatga cgtccgaatg gccgttcagg tacttcgtgg cgctgtgcag caccacgtcc
     3181 acgccctgct ccagggggcg ctgcagcacc ggggtggcga aggtgttgtc gatcagcacc
     3241 ctgcacccct tctcatgggc cagcctgcat gtctctttga tgtcgatcag cttgagcagc
     3301 gggttggtcg ggctctccag gtacaggacc ttcgtccggc cgtcgatggc cgcgcatagc
     3361 tcctcggggt ccgtggtggg gacgaaggcg acgtccacgc ccagccgggg catctcgttc
     3421 ctcaggagcc cgtacgtccc cccgtacagg tcctgcatcg cgacgaccct gtcccccttc
     3481 ccaacgaagg cgaggagggc ggtggagatc gccgccatgc ctgatgagaa cgccaatgac
     3541 gcctcggccc cctcgagcga ggccagcttc tcctccaccg cctcgaccgt ggggttgctg
     3601 ttcctggaat agatataggt ccccctcgga acctccccct cccacgtcct cggatcgtcg
     3661 gtggggaagg agaacgtgct gctttggaag atgggggtgt tgatggcgcc ctcgtagcgc
     3721 agggacctgt ccccgtcatg caccgcacgg gtggccatgc ctttcttgga catggagagg
     3781 tggatccccc ggcagcgcct taatcgttcc tctcactccc ctgtgcggcg gattatgatc
     3841 gaaaggacgt caccgtccgc gagcctgtgc tccagcccca ccatctgccc cgggaatcgg
     3901 gcgctgtctc cccataccag agcgtaccga aagttctgcc ggaagacccg gtgcagggaa
     3961 tcgcagacgt cgccgacggt gctgcccttc ttggcgatca gcggcacgtc catgtcggcc
     4021 tcctgcccct gcttcttcat gtagatgcgg atgagttcca gtcgctcgaa gatcttcgcc
     4081 ttcagctcgt ctatgcctat gttcttggcc gcggagatga ggaccgggtc ccaatccttg
     4141 aacctctcgc ggacctcctc caggtactca tcgtccacca ggtcgatctt gttgatggcc
     4201 agcaccgcat tgatgtagat gcggttcccg gagaggacgt ccacaagctg gtcctggtct
     4261 atgtcctcgc ggatgaccac gtcggcgttg accaccccgt actccatgac catcgccttg
     4321 gcgagttcca ggtccagctt ggtgagcttg accgtgctct tgacctccac ccctccggtc
     4381 tcccgctggg agatcacgac gtcgggggga tgggcattga tgcgcatccc ggcgttctcc
     4441 agctcgcgca cgagcacctg caggttggtg tcgtagaagt ccagcacgaa gaggatgagg
     4501 tcggaggacc ggacgaccga gatgacctcc cggccgcgcc ccttcccctt ggaggcgtcc
     4561 ctgatcagcc cgggcatgtc caggacctgg atcttcgctc cttggtactc gtacgccccc
     4621 gggaccacgt ccagggtggt gaagtcgtag gcagccacct cactcttggc ccctgtgagg
     4681 gtgttcagta gggtggactt cccaacgctg gggaagccga ccagggccac ggtggcattg
     4741 ccgctcttct tgaccgaata gcccttggca gagcctccgc tggaggccct cctccgctcc
     4801 atatcgtcct tgagcttggc catcctggcc ttcagcttgc cgatgtggta ttcggtggcc
     4861 ttgttcttct gcgtcttgtc gatctcttcc tggatcgcct tgatctgctc ctcgatcgtc
     4921 gaggccatct ggtcctaccc tcgctccact gtggtgcctg ccctcccttc aagggcctcc
     4981 atcagccggt cggccgaggc gatgaccgcc cggcgcccgg tggcctccac gaacctgacc
     5041 gccgcctccg ccttggggcc catcgacccc gcagggaact gcccctccgc cgcatggcgc
     5101 cgcagctcgg acgccgtcgc cctgagcagg gggcgcgggc ggtcgctcgt gaagtccaga
     5161 tagaccgcct caacatcggt caccattatt aaaatatcgg cctccaggat gatggccaat
     5221 cgggccgagg tgaggtcctt gtccacgacc gcctccactc cgcggtagcc actgccatcc
     5281 tggacgacgg gcacccctcc gcccccgcca cagagcacga caaagggagg ggggagggag
     5341 gcgcacaagg ccttgagatg cccctcctcg gccaccctga ggacgtccgg ggagggcacc
     5401 accctgcgca tgcccccgcg cttcgggtcc tgcctgaagc tccagccctt ctcccactgg
     5461 agccgctccg ccctctcctt ggggtaatag gggccgatcg gcttggtggg ggagaggaag
     5521 ctcggatcgt cggcggcgac cacggttcgg gttatcatgg ggagcatggg cacctccctg
     5581 cccgccctca ggcactcctg gtcccaggcc tgcagcgcca gatacgctat ctgcccctgg
     5641 gactcggcca cgcagacgtc gaggggcatc gccggcacct ccgcccggca cagctcgttg
     5701 cgcagcagga tgttcccgac ctgggggccg ttgccgtggg tgagcaccag gtggggatat
     5761 tggtccagca gcgggagcgt ccgccggaag acctcccgca tgttggctat ctgcacgtcg
     5821 cccctggcct cctggccgga cctaagtatg gcattgcccc ccagggccac cacggccagc
     5881 cccccgccgc cccctcccgg ccctgtcatc gcctcaacga aagggtttaa agatacaata
     5941 attatggccg gacgatgtct ttcatcgacg atatcaataa gatgctgaag ggggtccgcc
     6001 aatccaagat gtccatctgg atagggatgg ggctgaccat cctggtcacc gtcctcctta
     6061 cccttactat ccagctttgg ctgtcccagg tcgtctgtct cctgcccatc ctggtggcct
     6121 tgctcgccta ttatcttccg acctatttcg gcctgaagga ccgcaagaag ctcgccctct
     6181 ttggtctggt gctgttcctg gtcatcgggc tctcgctggg ggcggcgctg tacaagagcg
     6241 tggaggggta cgaggtcgtc gagctcagca gcgatgatga cctcctcgtc caaggaacgg
     6301 tatccccatc ccagggcgcg cccggggaca tcttcgattt cagcgtcgtg ctggtgtccg
     6361 gaaatgcgac tgcggacgtg cggctgttgc tctacaacaa ttggggcgat ccagatgatg
     6421 cgctgaacat ctccatggcc ttctcccaca acgtcaccct ggacgattcc tccattggcg
     6481 ctctgtacac gaccaacacc agcagcctgg aggaggggat ctaccacttc cagtacggag
     6541 cgttggacgg gggcgcatgg accgagacgg agcagggctt cgggccggtc aacgcggagc
     6601 tgggcaaggt gctcagcaac tccctgatgt ccggcgtact atactcgttc tacagcatgg
     6661 ccgtgctgtt ctacatcctc atcctgctca cctggtggat ggaccgctcc aagaagaaga
     6721 tggccgagat ggaacagcag cgggccaagg tcaaggagaa gaagggggcg gaggagaagt
     6781 tcgtctgctc tgactgtgga gccgaggtgc ctagggacgc ggacaagtgc ccggcctgcg
     6841 gggccaagtt cgatgacgaa gagttcaagt gcccgcaatg ctccgcaaag ctcctgcgga
     6901 ccgacaccaa gtgctggaac tgcggcaaga agctctgagg gcgaaaaaca ttttagcgcc
     6961 cggggggata tgcaccccgg gatgcacctc gatttttctg aggaagggga gaagcgatgg
     7021 gttcgatagc cgatgagctg gcgaaaaagc agaaggagat atccgtctcc gagttcttcg
     7081 agcgcaaccg ccacatattg ggcttcgact cccaatcgaa ggcgctgatc atgggcatca
     7141 aggaggcggt ggataactcc ttggacgcct gcgaggaggc ggatgtgctc cctgagattg
     7201 tggtcacggt ggagaggacc gaaaaggacg agttccgggt cagcgtggag gacaacggcc
     7261 cgggaatagt caagaaggcc ataccgaacg tgttcggccg cctcctgtac ggctcccgct
     7321 tccatgcggt acgccagtcc cgcgggcagc aggggatcgg gatatcggcc accgtgatgt
     7381 acggccaaat caccacagga aagcctgcgg tcgtcatctc caagacgggg gcggacgacg
     7441 tggcctggcg catggagatc atggtcaaca ccaaaaggaa cctcccggat gtgctgaagg
     7501 aggacccctt catctgggac ggaaaggagc acgggacgaa gataacgtac catatgaacg
     7561 ggcggtatgt aggggggagg cagtcggtcc tggagtacct ccggcagacc gcgatcgtca
     7621 atccgcacgc ccggatcacc ttcacggacc ccgacggcag gcgcaccacc ttcgaaaggg
     7681 ccacggacag gatgcccccg gcgaccaagg agatcaagcc gcacccttcc ggactggaac
     7741 tcggtaccct catgagcatg gccaaggaga ccaagaacaa gtccttgggc agattcctcc
     7801 agctggactt ctcccggata agcgatcgcc tggccaaaga catctgcgaa agggcggggc
     7861 tggacatgat gaccgacccc accaggctga cattggagga gcacaagcgg ttgctcgccg
     7921 ccataggcac ggccaagatc atggccccgc cgacggactg cctttcgccc atcggcgccc
     7981 agctgataaa gaaggggctg cgcaacgtcc tggacgaggt gcgccccgag ttctacgccc
     8041 cgccgatcac gagggacccg aaggtgcact ccgggaaccc gttcctggtc gaggtgggga
     8101 tcgtgtacgg gggcgatctg cccaaggacg cccccgtgca gatcctgcgc ttcgccaacc
     8161 gcgtcccgct tctctaccag cagggcgcat gcgccatcac caaggccgta gagagcgtcg
     8221 actggaggcg gtacgggctg gaacagcgcg gcggctccgg catcccgttc ggaccggcta
     8281 ttatcctggt gcacgtcgca tccaccaagg tgcccttcac ctccgaggcc aaggaggcca
     8341 tagccacaat cccggagatc caggcggaga tcgagctggc gctcaagatc tgcgggcggt
     8401 ccctgaagac gcacctcaac aaaaaggaga cgaagagcaa gacccgggtg aagtttgaga
     8461 tcgtccagga gatattgccg ctcatcgccc agaagagcgc caagatcgtc ggcaagccgg
     8521 tcccgaagct cagcgggagc ataaccaaga tcatgaacgt ggtctgggta gacgatgcgg
     8581 tctccttcga gaaggggcat catcgcatcc gggtctccat atacaactat accccccaat
     8641 cgcaaaagtt caatctgcac atggtcctcc cgcgaggcgc cttcgattac aaggggctgc
     8701 agttcttccc cactgaggtc agggaggacg gcaaggtcac ctgggagctg ccgaagatcg
     8761 cgtccaccga gaggctggac atcaactttt cgctgagcgg cctcaacaag gaggactatg
     8821 acgagaacga gatctacgcc tcgggcatag acccggtggc gatcataggg gcggagccgc
     8881 tgccggggga ctgggacctg aagggactgg aggtgaccga gagcgtggag gccaaggggc
     8941 cggcgcccga ggaagaggag gaagaggtcg actacgacga ggcgaaggag gcgctgaacg
     9001 atgactgagg aagagaacag ggcggtgcgc tcattgaaag agatcgcagg gtcgatctac
     9061 gaccagatag attcggggaa ggtgccgaag atgaccctgc ccctgaggac caagagcaac
     9121 atccggttcg acccgaagca cggggtcttc aagtacggca aggccacggg cgcccgctcg
     9181 gccaagaagg tcaatggggc gctgatgctc ctgcgcacca tgtacgtcct ggagttcata
     9241 gaggacatgg tccagaacgg caagtcctcc accctcaggg agatgtatta catctccgag
     9301 ggctgggaga aggccaagtt ctcctcccag gacgagagca acatgctggc cgaggacctg
     9361 gagatcgtga ccgcctgcat gcgggaggac ttcaagctgc gggcggagga gaacggggcc
     9421 agcatcatcg gggacctgac gataagcgag atggaccgca aggggaaggt caagaggata
     9481 aactgccggg acgatgtggg cgactccggc tacggcatcc cgaacaatgt ggagagggag
     9541 aaggtgcagc tcctgggcac caacgccaaa ttcgtggtcg ccatcgagac cggcggtatg
     9601 ttcgaccggc tcgtggagaa cggtttcgac gagaggtaca acgcggtgct ggtgcacctg
     9661 aaggggcagc ccgcccggtc cacccggcgc ttcatcaagc gcttgaacga ggagatgagg
     9721 ctgccggtgg tggtgttcac ggacggcgac ccctggtcct tccgcatctt cgcctccgtg
     9781 gcctatgggg cgatcaagac cgcgcacatc tccgagtatc tggccacgcc gaccgcggag
     9841 ttcgttggca tcaccgcctc cgacatcacc aactacgacc tgccgacgga caagctgacg
     9901 gacgtggacg tccgggcgct caacgccgaa ctgaaggacc cccgcttcgc ggacgagtac
     9961 tggcgggggg agatcgagac catgctcaag ctcaacaaga aggccgagca gcaggccctg
    10021 gcgaagtacg gcctggactt cgtcactgac aggtacctgc cggagaagct gagcaacatg
    10081 ggcgttctgc gctgaccacc agggcaagat tgatagtgcg gccgtgccat tgtccgggct
    10141 gaagatgaac gatcatatct cgatcaccct gacaagcctg acggcctcct acatcatcat
    10201 cgccaccgcc gcggccatcc tcattgcctg gctgacggag gattggaccc tgttcatccc
    10261 atccatgctc ctgctcgggg gcgccttcgc aacctacatc ggcctgaagc agcgcaccag
    10321 gcccctgagc aggacggaaa ggggcaacgg aaactttctg atgttctggg gcacgttcct
    10381 tatagccatc agtttgatct gggccatcaa ctacgtgtac ccgggcaacg ggctgctcct
    10441 gttcatcggg ttgctggtgt ggctcgggat cgccgtggtc ctcttcacga tgaagcgggg
    10501 ataggcaggc accgaaggcg gccggaaggc attgcccgac gtcctcgtcc atcgccaaac
    10561 ggctccggtc atccttgatg aggacagggg cggacgctcg gccgatccga gcgccccggc
    10621 gccctgggcc ttagatcggt cgagggctgc cttgggaccg gacgggctca ggcctctgcg
    10681 aggcgtttga gcacgaagcc cctgtccaga gtcgagagga agaaccccag ccgggggccg
    10741 gaggtccggg cgatgaacag gcggtacagc gcctggaagc aggccttggg ctgcatgccc
    10801 atcgccttcg ccgtctcgta tatggcatcg tggatgcggt cccccgtcca ttcgatgcct
    10861 tcaaggcgct ccctcagcgc ttgtacgtaa gcccgctccg cctcggtgag ctccacgtcc
    10921 gggcgctcgg ggctgacggt gaacaggacc tcctcgggcg cgaaggcgtt gagccagtgg
    10981 cgcacacagt tcagcctctg cttgagcacc tccatgtcct cctccgagat ctcggggatg
    11041 ctcatggagc gcttcaggac ggcgacggcc ccctcgaacg tgtccgctat ctgggccacg
    11101 ctgaccagat gacggtaggg gacctggagg gggagcctcg agcgcacccc cgagggctgg
    11161 gccagctcgt aggcccgaag gaggtccctc tccttctcgt ccgctccccc gttgaagtag
    11221 agggcctcga ccttgtcgta ctcgtccacg atgtcgagga tgcccatgcc agggtcataa
    11281 tcgatgtggc gctcggggtt gtagcgcagc acgctgaacg ccaggaccgg ggccggggtt
    11341 atgaggaggg cgtcggtccc ggtcacgaca ttgccggtgg acttgtgcat ctggcccctg
    11401 cccttgaact ggatgaactc atagggcacg ggatacgggg gctcggcgcc atagacctcc
    11461 ctgcagagcc ggacaccgct gtcgtacgag cctccggcgg ccgcgtggtc cttgccgaag
    11521 ggctcgcagg tcaccccgaa cagcttccac ttggccggcc attccacccg ccagggcatc
    11581 ttgccctggc ctttcctaat gtccgcatcg ccctcgtagc cgcaccggca cttgaagtga
    11641 acgtagggga actcgtagct gacgatcctg tcggagaacg tcccgcactc cgggcattct
    11701 gcgctgaagg gccagaagcc gtccggcatc ttccgcccgg tgacctctcc gaggatggcc
    11761 gccaccttgg ccctttccct gaggacgagg tccacgatct ccgtgaacct gccctcctcg
    11821 tacagcctgt gggtccagat gatccgaggc cttatgccca gctcccctat ggcgtccagg
    11881 aagggcgtga tgtagtgctc ggcatagctg gcatggcagc cgtccgggca ggggatgtag
    11941 cagatcggct tcccgacctc cttctcgaag cgctccggaa ggaagggata gcgcttgcgc
    12001 aggggatcgt aggaatcgac gagatagatg aaatcgacat ccgccttccg ctccttcagc
    12061 gccttgtaga cggcgttggc ggttatggac tcccgcaggc tgcccacgtg aatgtagcca
    12121 gagggggtga tgccagttga tatcaggtgc ttccctcccc tttcgacgag ggcgttcgcg
    12181 atcacatctg cccaatgcat gctatcgagc cgtgaagccc cctgtccgtt ataaggtttg
    12241 tcgcggttgg acaggggccg ttcctttccc atccctgcaa aattctttta tatcgtttcg
    12301 cgggtagcat gtccgataga catggcgaag gagaacaaga ccggagggtt ccaatctgct
    12361 gcgggcctta taaggtattt cgacagcgag gacgagaagg ccctgaagct gagcccctat
    12421 gtggtcatag ccctctgcgt gatcaccatc gcgctcgtcg tggtggccag cgccctgtat
    12481 cccacttgaa cggagctttc gatcccgggc gggcagggaa gacgggcttt catgtggtct
    12541 tctatggccc gctgatccca tgatcacaat gtccaacggc atgccctgac gtagggggca
    12601 gggcttccta tgtacgatga tcgaggcctt gctcctacaa cgatcctcat gcacatccag
    12661 aagctggata gaccctctgt tatggaaaaa atgtcgaagg gtaaaggaat ggaagggggc
    12721 tcagttcacg tcgggcggca gtacctcaca cttcttgccc agcttcctct ggaaggtgta
    12781 agtgagcagc ggaggggcca ccagggtggt caccagggac atgaagacta tggcgttgta
    12841 gaggccgggt tcgatgatcg acagaccggt ctcggggtcc aacatgccca ggccaatgct
    12901 ggcaacgatg aggccgacct cgcccctggg caccatcccg acccctacga tcgctgcgga
    12961 cttgcccccg aggctcctgg cccccaaccc gcagccgatg aacttcgtga tgatcgcgaa
    13021 gacggttatc gtggccgcca tcatgagcac cccaagttcg ccgaaggcct ctaggtcgac
    13081 cgcgatcccc acgaacacaa agaagaatgg ggtgaggaac tccactatcg gctccatctt
    13141 ctcctcgcac ggccacctgt ccttgaactc ggcgaacgcc attccggcca ggaaggcgcc
    13201 gatgatcgcc gccagcccaa ggaatgagga tatggacgag agggcgaagc acacgatgag
    13261 cgctagcggg agggggctga tgcccttcgg cctacgttcg gccgtgcaga cctgggtcac
    13321 cttcctatcg tccctcttct tgagcacctt cggcaggacg agggatccga tgaagatgat
    13381 cgccagcaca aagagcacgg cctcgatggc gaccaccgcc acctggaaga tgtccaggct
    13441 gccgcctcca gcgatgccga ccacgatggc gaggactatc atgcccaata tgtcatcgat
    13501 gaccgccgcc ccgattatga cccgggactc cacggtatgc atgaggttca tgtccctgat
    13561 gacccgggcg gtgatgccca cgctcgtggc caccatggac gcgcccacga acagggcctc
    13621 gagctgcttc tccggaccga gggcgccgat catcaggaga tacccgacta tgaacgggag
    13681 gatgaccccg aacaccgcca cgaacatggc ggtcttgccg accttgcgga tggcgctgaa
    13741 tggggtttcg agaccaacgg aaaagagcag gaatatcaca cctagctcct tgaggacctc
    13801 gaagacctcg cggtcggaac cgaggttgat gaaatcgtag aggaaggtgt tggcgatgag
    13861 cactcccacc aggatctccc cgatgaccgt ggggaccttc accttgttgc agagcatcct
    13921 ggccacttcg accagtacga tgatcacgaa gagctggaac agcacggtct ggatgaagtc
    13981 ctctcccatc ggggtaaccg tatggtttat ttcctatatt aagttctgag cgagcgccca
    14041 gccagccccg ctgttcgttc accgggacgg tcatcattgc tcctggggga ggggagatgg
    14101 gtcttcgtgt tgaacggaaa agcataagta aggctaggcg atcgaagcgc tcgaagagga
    14161 aa
//
LOCUS       DAOSYN010000015        14074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_3649_length_14074_cov_4.289821, whole genome shotgun sequence.
ACCESSION   DAOSYN010000015 DAOSYN010000000
VERSION     DAOSYN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 14074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14074
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3649_length_14074_cov_4.289821"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLJ11_01370"
     CDS             complement(<1..382)
                     /locus_tag="PLJ11_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOO03355.1"
                     /translation="MTGSEFDLAFFKEQGFKRTQCPKCKRYFWSLGDWATCGEPPCEE
                     YTFIGHSPMNQRLTLHEMREAYLSFFEENGHGRVKRYPIVARWRDDVFFTQASIYDFQ
                     PWVLNGVIEPPFNPLTISQTCVRFN"
     gene            complement(452..928)
                     /gene="msrA"
                     /locus_tag="PLJ11_01375"
     CDS             complement(452..928)
                     /gene="msrA"
                     /locus_tag="PLJ11_01375"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOO03356.1"
                     /translation="MTLRKATFAAGCFWGVEAAFMKVRGVVHTEVGYMGGHTESPTYR
                     EVCTDRTGHAEVVHLTYDDSLISYTQLLDVFWNSHDPTQKNRQGPDVGTQYRTAIFYH
                     DEEQRRTAERSMATLAASGRFDRPIATQLEKAGPFWRAEEYHQRYLQKKGLDTCHI"
     gene            complement(972..2549)
                     /locus_tag="PLJ11_01380"
     CDS             complement(972..2549)
                     /locus_tag="PLJ11_01380"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HOO03357.1"
                     /translation="MRASRLLVDCLRAEGVDRAFGIPGEENVDLMDDLHSGGMEFVLT
                     RHEQAAAFMAATVGRLTGRPGVCVSTLGPGATNLATGVADAHLSHLPLVALVAQAGAA
                     RRDPPQKQVLDLLALFRPITKRAMDVGGPGDVPASVRRAFALAARERPGPVMLQVPED
                     VMGMEASGEPIGPPKVKARRAPAARLEEVRALLRTAERPLVLAGHGVLREEACEELRA
                     FCEAWGIPVGCTWMAAGALPYDHPLSLGTVGMRDSDLVRASYEEADAVLLVGFDCPEF
                     QPQYWDRGSRKRIAYLGRAPPMRCPDLGLEIVAVGDLVHSLGALTRGAERKDSWHAVH
                     REDLLREVNREGEGPRAVVKALRRQMGRADIVVSDVGAHLIWLAKYYPAYGPNTLLLQ
                     NGLISMGVAMPSAIAAKLVHPERRVVAAVGDGGFLMASAELETARRLGVRFVTVVFND
                     GGLGLIREKMQRRLGRSSNVDLGLPDIPTYARSFGAEGYSVGEREFEEVLRDCLARDA
                     LAIIDVKVDYSYNRSLF"
     gene            complement(2554..3861)
                     /gene="nifB"
                     /locus_tag="PLJ11_01385"
     CDS             complement(2554..3861)
                     /gene="nifB"
                     /locus_tag="PLJ11_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HOO03358.1"
                     /translation="MQERGTEGPSESTISEEVREMLRTHPCYNEGAHRSFARMHLPIA
                     PRCNVQCNYCNRKYDCMNESRPGVTSEILSPEEAVAKIRIVKERIPQLSVIGVAGPGD
                     PLANEETFVTLEMVRKEFPELTLCLSTNGLNLPRSVERLKALGVRFVTVTINAVDPEV
                     GARMYDFVLWEGKVLRGREGAERLLANQLEGIRMCTAAGILVKANIVMVPTVNADHIP
                     AIARKVRELGAYIVNIIPLIPVPGTRFEHMPAPTPEERKRLQDMCEGEIRQMRHCRFC
                     RADAIGMLDNDRSAEFAHITCGAREAPEKVPFGVQMEGAAKHRVAVATSNGRNVDLHF
                     GQAKEFWVFDVEGGTLVPAGKVRAEPFLDEPMFGPVHRSKIENMVAALKGVDVVLASG
                     FGERAEAEIRARGIYPYKREGALEEAVLCAVRELFEERARVFE"
     gene            3954..4361
                     /locus_tag="PLJ11_01390"
     CDS             3954..4361
                     /locus_tag="PLJ11_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF61 family protein"
                     /protein_id="HOO03359.1"
                     /translation="MSDLLNERTMQGLFTSMNQHIPVKRRTLEQLLSDPEPSYQARDG
                     RVYRLDRSELLLLFELLGPEERSSLKLPILIMTDTAYGEGYWKVMGRAEVKALSALIG
                     REPEREDEMRVFYPYLKEIRDRLPTTTTTVFSY"
     gene            complement(4522..7427)
                     /locus_tag="PLJ11_01395"
     rRNA            complement(4522..7427)
                     /locus_tag="PLJ11_01395"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
     gene            complement(7827..8702)
                     /locus_tag="PLJ11_01400"
     CDS             complement(7827..8702)
                     /locus_tag="PLJ11_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOO03360.1"
                     /translation="MKGSALLKDRTYLVQGITGKQGSYHTRQMLDYGTRIVGGVTPGK
                     GGQTVHGIPVFNDVQRAMARTGATASVAFVPAANLKDAVQEGLEAGLEAMVLITERTP
                     IHDTMGFLAMARRRGTAILGPNCPGLCIVGESKVGIMPNHIFKKGGLAIISRSGTLTY
                     EVVQALSSAGLGQDVVLGLGGDRLLGTDMREAVEIVLERGPSGVVMIGEIGGQMEEEA
                     SLLIKELGAENIFAYVAGVSAPPARRMGHAGAIVSRGSGGAYEKIKALEGSGVAVGRS
                     LGDLVTLISKRIERP"
     gene            complement(8699..9826)
                     /locus_tag="PLJ11_01405"
     CDS             complement(8699..9826)
                     /locus_tag="PLJ11_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOO03361.1"
                     /translation="MNLLEYEAKHLFRQYGISTPKGLSFKDMEGISSALTNLDFPLMV
                     KAQTTVGGRGKAGGIRTALYKQEALEAAERILSMRIGGMKVHGVLLEEMVEASREIYL
                     AITIDHRTRCPLLLAGSAGGMDVENMPAHTIRRWSLDPLEALPPEVLEQASRFLLRGE
                     TGTQEVRSTIQAMWRLFRGMDCELVEINPLMITDSGKAIAADAKVAIDDEALFRHPKF
                     MERAGRDLTELERLAKERGFTLVEMAGDIGVLANGAGLTMAALDTLQRYGGRGGTFLD
                     LGGTDDPDRISEALGLLSYDLAKGRTRSILVCIFGGITRCDTVADALMHTITDRNVKA
                     RTVVRLRGTNEEEAVRMLQLANFNVQIDLDEACLQAIKVRA"
     gene            9943..10584
                     /locus_tag="PLJ11_01410"
     CDS             9943..10584
                     /locus_tag="PLJ11_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO03362.1"
                     /translation="MVVRCLAGMDFDSNVYLLTGEDPILVDAGTGMHHQDIVRWLSFT
                     ARQSKVRRIVLTHRHYDHTGGAAALAKELRAEVLMHPGDAPAVRDGDVHQTLAYMFGA
                     PGQPVPVTDLREGEVLSTGDASYEVLHTPGHTSGGLSLWDRENRVLLPGDTVFVGGVG
                     RWDLPSGNFEQLVGSVKKLLSLSPQELYPGHGDFCQKGAEGVILEAIKYLGES"
     gene            10585..11226
                     /locus_tag="PLJ11_01415"
     CDS             10585..11226
                     /locus_tag="PLJ11_01415"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOO03363.1"
                     /translation="MDIIKCKEGKSTEKGLSKHDLEHVLSTLRFGRLVVYPTETVYGL
                     GADPFDETAVKRVYLAKRRPFDMALSIAVHDISVADKIAELDENAYRLAKAFLPGPLT
                     LLVKKRPIIPDIVTAALPEVGIRVPDHPIALHLLEEFGPIISTSANIHSHPNPVRVEE
                     AIADLGNSVATYVDGGPCKVGVPSTIVQITEGTVEVVRYGAIPKEKIENVLTG"
     gene            11234..12109
                     /gene="map"
                     /locus_tag="PLJ11_01420"
     CDS             11234..12109
                     /gene="map"
                     /locus_tag="PLJ11_01420"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HOO03364.1"
                     /translation="MECIHKAGRLSSEARELGRSLVGEGIKLRDVAEAMESHILEHGG
                     RLAFPVNISLNDTAAHFTPNTIDDMVFQRGDVVKLDVGAHVDGYLGDTATTVEVGTRN
                     WQPLIRAAERALEMALEMAKDGVTVGTIGGVVERSIKEANLRPVVNLSGHEMKRYNLH
                     AGLSIPNIDDGTLTKVRENMLLAIEPFATNGQGQVSNGRSGNIYRVLRDRPLKDPDAE
                     RLFGRIKEEFTTLPFCERWCDRLQPNSGPLLRVLLRHGVISTYPVLVEAKQGMVSQAE
                     HTVLVGNGRNEITTA"
     gene            12154..12263
                     /locus_tag="PLJ11_01425"
     tRNA            join(12154..12177,12200..12263)
                     /locus_tag="PLJ11_01425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12212..12214,aa:Leu,seq:caa)
     gene            complement(12538..12825)
                     /locus_tag="PLJ11_01430"
     CDS             complement(12538..12825)
                     /locus_tag="PLJ11_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03365.1"
                     /translation="MPAIPALGKYLEKLSKWLGRFQTIIGIIAIILALIWWEDLLQSV
                     VALIAGLILITGILPSIPALGKHLEKLAKWLGGFQTIIGVIAIIVGIIGLL"
     gene            13260..>14074
                     /locus_tag="PLJ11_01435"
     CDS             13260..>14074
                     /locus_tag="PLJ11_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03366.1"
                     /translation="MVDERPAVMKKNPIRALMFKDALLEEIVDGLKAGQIPKEDMDEA
                     LFLTTTNAILEIIGMNLPEDLQLVFVENLDDYLSIAMINKEHAVDLLGQFRDGFLASH
                     EDSFEDEKALLEALTDYEEEWWGSPKDFLGGAVPNDLMEEARKRMDTILEQEEGADQE
                     CEGEEEEDYWASRSYVIRDLWFPSLLDSITKEPLPSPQRREVLFTAAANAILDIAVNV
                     MPDFMSEEMFEGLDRYLELTLVNKECKVDVLTIFEDALAKFENEWWTTGLKELG"
BASE COUNT         2885 a         4404 c         3777 g         3008 t
ORIGIN      
        1 cattgaaccg tacgcatgtc tgggagatcg tcagcgggtt gaacggcggt tcgatcaccc
       61 cgttgaggac ccacggctgg aagtcgtaga tcgaggcctg ggtgaagaat acgtcatccc
      121 tccaccgggc tacgatggga tagcgcttca cccggccatg cccgttctcc tcgaagaagc
      181 tcagataggc ctcgcgcatc tcgtgcaggg tgagccgctg gttcatcggg gaatgcccga
      241 tgaatgtgta ctcctcgcag ggaggctcgc cgcacgtggc ccagtcgccc aggctccaga
      301 agtaccgctt gcacttgggg cattgcgtgc gcttgaaccc ctgctccttg aagaaggcga
      361 ggtcgaactc cgagcctgtc atagctggcg ttattccggg gtcgttataa gaatattgtc
      421 atgatagcgc cctctcaggc gtcaccccca gtcatatgtg acaggtgtcc agccccttct
      481 tctggagata cctctgatgg tactcctcag ccctccagaa cggtccggcc ttctccagct
      541 gggtggcgat cggcctatcg aacctgccgg aggctgccag ggtcgccatg gaacgttcag
      601 cggtcctgcg ttgctcctcg tcatgataga agatggccgt gcggtattga gtgcccacgt
      661 ccggcccttg cctgttcttc tgtgtcgggt catgggagtt ccaaaagaca tctaggagct
      721 gtgtgtagct tatcagcgag tcatcatagg taaggtgcac gacctccgca tgccctgtgc
      781 ggtccgtgca cacctcccgg taggttggcg attcggtgtg gccgcccata tagccgacct
      841 cggtgtggac cacccccctc accttcatga aggccgcttc cacgccccaa aagcatccgg
      901 cggcgaacgt agccttcctg agcgtcatac tcccctcagg gatcgaacgc agatgcagct
      961 atataaccgc ttcagaacag ggatcgattg taggagtagt cgaccttcac gtcgatgatc
     1021 gccagggcgt cccgcgcgag gcagtcccgg agaacctcct cgaactccct ctccccgacc
     1081 gaatacccct ccgccccgaa ggagcgggca taggtcggga tgtcgggaag gccaaggtcc
     1141 acattggaac tcctgcccag gcggcgctgc atcttctccc gtatgaggcc aaggccccca
     1201 tcgttgaaga ccacggtcac gaagcgcacc cccagcctcc tggcggtctc gagctccgcg
     1261 gaggccatca ggaatccacc atcgcccacc gcggccacca cccgccgctc gggatggacc
     1321 agcttcgccg cgatggccga gggcatggcc acccccatgg agatgagccc gttctggagc
     1381 agcagcgtgt tcggtccata ggcaggatag tactttgcaa gccagatcag gtgggcgccc
     1441 acatcgctca ccacgatgtc ggcccgcccc atctgcctcc gcagggcctt gacgaccgcc
     1501 ctcgggccct cgccttccct attcacctcc ctaagcaggt cctctcgatg aacggcatgc
     1561 caggagtcct tcctctccgc ccctctcgtc agcgctccca gggagtggac aagatcgccc
     1621 accgccacga tctccagccc gaggtcaggg catctcatcg gaggggcccg gcccaggtag
     1681 gcgatccgct tcctcgaccc ccggtcccag tattggggct ggaactcagg gcaatcgaac
     1741 cccacgagca ggacggcgtc cgcttcctcg tagctcgccc tcaccaggtc gctgtcccgc
     1801 atgcccaccg tgccaaggct cagggggtgg tcgtagggaa gggcccccgc cgccatccag
     1861 gtacatccga ccgggatgcc ccacgcctcg cagaatgccc tcagctcctc gcacgcctct
     1921 tcccgcagaa caccgtggcc ggccagcacc aagggtcttt ccgccgttct gaggagcgcg
     1981 cgcacctcct ccagtcgggc cgccggagcg cgcctcgcct ttaccttggg cggtcctatc
     2041 ggctcaccgg aggcctccat ccccatgaca tcctctggca cctgaagcat caccggcccc
     2101 ggccgttccc tggcggccaa ggcgaaggcc ctgcgtaccg atgccggcac atcccccgga
     2161 ccgcccacgt ccatcgcccg cttggtgatg ggacggaaca atgcaagcag gtccaggacc
     2221 tgcttctgcg gagggtcccg gcgggccgcc cccgcctgcg ccaccaaggc caccaaaggc
     2281 aggtggctga gatgggcgtc agcgacgccg gtcgcaaggt tcgtggcccc cggcccgagc
     2341 gtgctcacgc atacgccagg cctcccggtg agccggccca ccgtggcggc catgaacgcg
     2401 gcggcctgct catgtcgggt gaggacgaat tccatccccc cggaatgcag gtcgtccata
     2461 aggtccacgt tctcctcacc gggaatgccg aaggcccggt ccacgccctc tgccctaagg
     2521 cagtccacaa gcaacctgga cgctctcatc tgatcactcg aagaccctcg ccctttcctc
     2581 aaagagctct cggaccgcgc agaggaccgc ctcctcgagg gccccctccc tcttgtaggg
     2641 atatatcccc ctcgcccgta tctccgcctc cgctctttca ccgaacccgg aggcaagcac
     2701 gacgtccacg cccttgagcg cggccaccat gttctctatc ttgctccgat gcacagggcc
     2761 gaacatgggc tcatcgagga acggctcggc gcgcaccttc ccggccggta cgagcgttcc
     2821 accctccaca tcgaacaccc agaactcctt ggcctggccg aagtgcaggt ccacgttcct
     2881 gccattggag gtggccacag ccacccggtg ctttgcggcg ccctccattt gaactccaaa
     2941 gggtaccttt tccggagcct ccctggcgcc gcaggtgatg tgcgcaaact ccgcggagcg
     3001 atcattgtcc agcatcccta tcgcgtccgc gcggcagaag cggcaatggc gcatctggcg
     3061 gatctcgccc tcgcacatgt cctgcaggcg cttgcgctcc tcgggggtcg gcgccggcat
     3121 gtgctcgaac ctggtccctg gcactgggat gagcgggatg atgttcacga tgtacgctcc
     3181 cagctccctc accttccggg cgatggccgg tatatggtcg gcgttcacgg tcgggaccat
     3241 cacaatgttg gccttgacca gaatgccagc ggccgtgcac atcctgatgc cttccagctg
     3301 attcgctagc agtctttccg ccccctccct gccccgcagc acctttccct cccagaggac
     3361 gaagtcgtac atacgcgccc cgacctcagg gtccacggcg ttgatcgtga ccgtaacgaa
     3421 tctcacgccc agcgccttca ggcgctcgac agagcgagga aggttcaagc cattggtgct
     3481 caggcagagc gtcagctccg ggaactcctt cctcaccatc tccaaggtta cgaacgtctc
     3541 ctcattggcc aaaggatccc ctgggcctgc caccccgatg acgctcaact gggggatccg
     3601 ctcctttacg atcctgatct tggccacggc ctcctcgggg gaaaggatct cgctcgtaac
     3661 ccctggcctg gactcgttca tgcaatcgta cttgcgattg caatagttac actggacatt
     3721 gcaccttggt gcgatcggaa gatgcatgcg ggcgaagctc ctatgcgctc cttcgttata
     3781 gcatggatgc gtcctcagca tctcccggac ctcttcggag atcgtgctct ccgatggccc
     3841 ctccgttcca cgttcttgca tcggcctgtg aatatccagg ggaccgtagt tatagattgt
     3901 cccaaccctt cttggaaaac gatataaatc gtgtcggagg tggtaatgct cgaatgagcg
     3961 atctcctcaa cgaaaggacc atgcaaggcc tattcaccag catgaaccag catatcccag
     4021 tgaaacgcag aacgttggag cagctgctga gtgacccaga accatcctat caagcaaggg
     4081 atggcagggt ctatcggctc gaccgcagcg aactcctgct tctcttcgag ctactggggc
     4141 cagaagaacg ctcctccctg aagcttccaa tattgataat gaccgacaca gcttacggag
     4201 agggttactg gaaggtaatg gggagggcgg aggtcaaggc gctctcagcc cttataggcc
     4261 gtgagccaga gagggaggac gagatgaggg tcttctatcc ctacctgaag gaaatacgcg
     4321 acaggcttcc tacgaccacc accacggtgt tctcctattg agaccgaggt ccaccagctt
     4381 gcgcctaaat ggtgatcgtg ctagacatag aatagtagaa gtgaaaacag cttaatgcat
     4441 cgggaaaggc cctggaagag ggtacacacg caacccgttt aacggttaag ccaaagccta
     4501 ccattaaggc aagcctgcag tgcttacgcg gcagaggacg ttagttaccg cggactgaac
     4561 atctcggatg gcctcgatgc ttacatcccg gttctatcaa gctcatcaac tatgagcgtc
     4621 ctaagatgcc tctttttcaa ggtggcttcg agcttagatg ctttcagctc ttatccatta
     4681 gagcgtggct gctcagcatg ccctgtcgga caactgattg actagaggct ccgaaccccc
     4741 gttcctctcg tactcaaggg tccttctcgt caggcatcaa ttcacttcca ccaaaaagca
     4801 acaaacctgt ctcacgacgg tctaaaccca gctcacgatc ccttttaatg ggcgaacaac
     4861 cccacccttg gcagctgctg caccaccagg atagggagag ccgacagcga agtagcaagc
     4921 cgccaggtcg atatgaactc ttgctggcga caactcaatt atccccaggg taacttttct
     4981 gtcatcaatc acccccattc agaaggttga ttggttcgct aggccctact ttcgtatcgc
     5041 cgatccttgt tgtccggatc cgcgtcaagc cagcttttac ccttacattc accggcagat
     5101 ttctgacctg cctgagctga ccattgggcg cgcttgttat ctttttgagc gcgtggcgcc
     5161 ccacccaaac tgcctaccta tcggtgtcct tctttcgaag ttagtggtac gaagttcgaa
     5221 gggcggtgtt tcatcgtcga ctcggagatt acctagcggc aattcctggg taacgtctcc
     5281 cgcctaccct acacatcgaa attcatatca caacgacagg ttgcagtaaa gctccatggg
     5341 gtcttcgctt tcaggtggaa gatactggac tgtgcaccag tactgttagt ttcaccggct
     5401 tcgatgcggg gacagtagaa ctctcgttgg gccttcatgc aagtcgccaa ttaagcgaca
     5461 aggtattacg ctaccttaag agggtcatag ttacccccgc cgtttaacgg tccttcgttc
     5521 ggttgtaccc gagtttcaga taccgtcact gggcaggctt caaccgctat acacatcctt
     5581 tcggacttgc agcgatctgt gtttttatta aacagtcgga gttcccttgc cactgcgacc
     5641 agccactctc gcggctggca ccccttatcc cgaagttacg gggctaattt gccgagttcc
     5701 ctcgcatcga ttaggccgac acgccttaga cttttcatct aggggcacct gtgtcggttc
     5761 ttggtacgaa cgcgtcaggt gtagatcgtt caattttcac ggacaccagg aatcagcaga
     5821 agaggacttt cgtcctccta ctcgcgcttt caccgagttc acaccattac ggttcttcct
     5881 cagtttatac gcttgaacaa ccagacaaga ttgctctacc tatcccgatg tgtcaagaac
     5941 gacgcagagt ctgacgcggc acaggaatat taacctgttt ccctttcact acttccgatt
     6001 aagtggtagt ttaggatcgg ctaaccctcg gctgacgaac attgccgagg aaccctggcc
     6061 cctacggcgg aacggattct caccgttctt tgctgctact cctgccatga tccttatacg
     6121 aacgcggtcc acaagacctc acggcctagc ttccacccac gcccgtcacc tccttaccag
     6181 atcaccttgc ggtgctcaat aatatcggtg ttcggcttag ccccgtccat tttcggggcc
     6241 tataatctcg actggtgagc tgttacgctt tctttaaagg atggctgctt ctaagctcac
     6301 ctcccagttg tcttggacta cagactccct ttattgttac acttaaccga aacttgggga
     6361 ccttaattat tggctgggtt acgttccctg atggacatca agcttacccc ggatgccctc
     6421 actcccggcc tctacggtga ccgcatgttc ggagtttgac agggggccga gggatttctc
     6481 cccctaagcc cccgatcagt gctctaccac acaatctacc tccgccgaga tctaactgcg
     6541 attagtttca ggaggaacca gctatttcca gctttgattg acctttcatc cctataccca
     6601 ggttatccga aagatttgca catcattacc ggttcggtcc tccaccttcc tttcgaaagg
     6661 cttcaacctg cccaggtata gatcaactgg cttcgggtgt ccctccaatg actccaggcg
     6721 agcgcacctc gtccctcgcc ttgcggctgc ggacttgttg gtttcccttc ggctccgagg
     6781 attatcctct tagcctcgcc attgaacaga actccatggc ccgtttttcg aaacggacgt
     6841 aaggacactg gtttcccttg catgccctta caatttatag ccgcctggtt tcaggctctt
     6901 ttaacctccc gtcaagggta cttttcagct ttcactcacg ctactactgc gctatcggtc
     6961 tcggggtgta tttagggttg gaaatgaatg tttcccaaat tcacgcgcga tatccaacgc
     7021 acgttactca gggacaccgg aactcatccc cttgatttat ccatacggga ctatcaccct
     7081 ctatggtact gcgttccagc agatctctgg ttggtcgtag ggatgtaacc aggcccataa
     7141 caccacatct ccccatcatt actggtgggg attcggtttg ccctgtaccg ttttcgatcg
     7201 cctttactca cggtatctct agtgatttct tttcctgcgg gtactaggat gtttcgatcc
     7261 cccgcgttcc cgttccttac ggaacattcc gaagaatacg aagtcgcatt aggtcatcat
     7321 cggatcatag gctccttgcg cctacccgac gcatatcgca gcttggcacg accttcttcg
     7381 gcacccgaac cgagccattc cccaggcggc gtagcaaacg ccgctggcac tgtcggctta
     7441 acaaacgtcc aggttgcgta tgattggtat caacgaacac ctcgggacct cttccttttc
     7501 cccgaggttc ctcctccact tccccggaaa gggtcccttt ccgggtgcac gcgcacttct
     7561 cacttcgccc cagcacatgg ctggaacgcg tgagtccccc aataattgag ggtatattta
     7621 agcattatcc accaggatat gctcccgctc gcctgcttcg cgagcggaac cggctaccac
     7681 agttccatta tataacggtt tcttacagca gccagcgtag gttgccaccg ttggtccgat
     7741 accgttctca ccggtcatta tcaattagta gatggtccct cataaggttt tctctcctaa
     7801 tggaaacgaa ccaatgtaca gaggccttac gggcgttcga tccttttgga gatcaatgtc
     7861 acaaggtccc caagcgatct tccaacggcc accccagacc cttccagcgc tttgatcttc
     7921 tcataggctc ctccgctccc tcgggagacg atcgccccgg cgtggcccat gcgcctggct
     7981 ggaggggctg aaacacctgc cacataggca aagatgttct ccgccccgag ctctttgatc
     8041 agtagggagg cctcctcctc catctgccct ccgatctccc caatcattac caccccggac
     8101 ggtcccctct ccagcacaat ctccaccgct tccctcatat ccgtgcccaa gagccggtct
     8161 cccccaaggc caagtaccac atcctgacca aggccggcag aactcagggc ttgcaccacc
     8221 tcataggtga gcgtcccgct cctcgatatg atggcaagac cccccttctt gaagatatgg
     8281 ttgggcatga tgcccacctt actctcgcct acaatgcaca gcccgggaca gttcgggccg
     8341 aggatggcgg tccccctcct ccgggccatg gccaggaacc ccattgtatc atggatcggg
     8401 gttctttctg tgatcaggac catggcctcc aaccccgcct ccagcccctc ctgcacggca
     8461 tccttcagat tggctgcagg aacgaaggcc acgctggccg tcgccccagt tctggccatg
     8521 gccctctgga cgtcattgaa caccgggatg ccgtgcacgg tctgcccgcc ctttcccggt
     8581 gttacgccgc ccactatcct cgttccatag tccagcatct gccgggtatg atagctcccc
     8641 tgcttgccag tgatcccctg caccaaatac gttcggtcct taagaagggc gcttcctttc
     8701 atgccctcac ctttatagct tgcaggcacg cttcgtccag atcgatttgg acattgaaat
     8761 tggccagttg gagcatgcgc accgcctcct cctcattggt cccccgcaac cgaaccacgg
     8821 tccgggcctt cacgttccga tcggttatgg tatgcatgag ggcatcggcc accgtatcgc
     8881 atctggtgat ccccccgaaa atgcagacca ggatggacct tgtcctcccc tttgcaaggt
     8941 cgtacgagag cagcccaagg gcctcagaga tgcggtcagg gtcgtccgtc cccccgaggt
     9001 ccaggaaggt acctcctcgc cctccgtacc tctgcagcgt gtccagggcg gccatggtaa
     9061 gcccggcccc attggccaag acgcctatgt cgccggccat ctcgacgagg gtgaaccccc
     9121 tctccttggc caagcgctcc agctccgtta ggtcccggcc agccctctcc atgaacttgg
     9181 gatgccggaa cagggcctca tcgtctatgg cgaccttcgc atcggctgca atagccttcc
     9241 ccgagtcggt gatcatcagg gggtttatct ccaccagctc gcagtccatt ccccgaaaga
     9301 gcctccacat ggcctgtatg gtagatcgta cctcctgggt ccccgtctcc ccccgtagaa
     9361 ggaagcggga ggcctgctcc aagacctcgg gaggaagggc ctccagcggg tccaggctcc
     9421 atcttctgat ggtatgggcg ggcatgttct ccacatccat gcccccggcg ctacctgcca
     9481 ggagcaatgg gcagcgggtc cgatgatcga tggtgatggc cagatagatc tcgcgcgagg
     9541 cctccaccat ctcctccaac agtaccccat ggaccttcat gcccccgatc cgcatgctca
     9601 ggatcctctc agcggcctcc aaagcctcct gcttgtacag cgcggtccga atgcctcccg
     9661 ccttccccct tccccctacg gtggtctggg ctttgaccat cagcggaaaa tcaaggttgg
     9721 tcagggcgct ggagatccct tccatgtcct tgaaggacag ccccttgggc gttgaaatgc
     9781 cgtactgcct gaagaggtgt tttgcctcgt actccaatag gttcatccca tctccgcctt
     9841 cccattggcc tcggacatat aagtgacttg ttcgaccaga cgaaataacg attggactgt
     9901 gggttcaaaa gtttatatag gaccataatt tatcgaaaag cgatggttgt cagatgcctc
     9961 gctgggatgg acttcgattc caacgtctat ctattgaccg gcgaggaccc aatcctggtg
    10021 gacgcgggca cgggcatgca ccatcaggac atcgtgcgat ggctatcgtt cacggcaagg
    10081 cagagcaagg tgaggcgcat cgtgctcact caccgtcatt acgatcacac aggaggcgcg
    10141 gcggccttgg ccaaggaact gagggcagag gtcctcatgc atcctggcga tgcgccagcg
    10201 gtccgggatg gagatgtgca ccagaccctc gcgtacatgt tcggcgcgcc cggccaaccc
    10261 gtcccggtca cagaccttag ggagggagag gtcctgagca cgggggatgc gagctacgag
    10321 gtcctgcata ccccaggtca tacctcggga ggcctgtccc tctgggacag ggagaacagg
    10381 gttctcctcc ccggggatac agtgttcgtg ggtggggtag gacgctggga cctgccaagc
    10441 ggcaacttcg agcaactcgt gggttcggtg aaaaagcttt tatccctctc tccccaggaa
    10501 ctttaccccg ggcatggtga tttctgccag aaaggcgcag aaggggtcat ccttgaggct
    10561 attaaatatt taggagaatc ttgaatggac ataataaagt gcaaggaagg caagagcacc
    10621 gagaagggct tgtccaagca cgacctggaa cacgtactgt ccacccttcg cttcggccgg
    10681 ttggtggtat atcctacgga gacggtctat ggtctggggg ccgacccttt tgatgagacc
    10741 gcggtgaaaa gggtttattt agcgaagagg aggccgtttg atatggccct ctccatcgct
    10801 gtccacgata tcagcgttgc cgacaagatc gccgagctgg atgagaacgc ctatcgtctg
    10861 gcaaaggctt tccttccagg ccccctaacg ttgctggtga agaagcgccc cataattccg
    10921 gacatcgtca ccgccgccct accggaggtt ggaataaggg taccggacca cccaatcgcc
    10981 ttgcacctct tggaggagtt cggtcctata atctccacaa gcgcgaacat ccattcccac
    11041 cccaaccccg tgagggtgga ggaggccatc gccgacctgg ggaacagcgt ggccacctac
    11101 gtcgatgggg ggccttgcaa ggtgggggtg ccatcgacga tcgtccagat caccgaggga
    11161 acggtcgagg tcgtccgcta tggcgccatt cctaaagaga agatcgaaaa tgtacttact
    11221 ggatgatcac gcagtggagt gcattcacaa ggccgggcgg ctatcctcgg aggcccggga
    11281 gctcggccgc tccttggtcg gggaggggat caagctccgt gatgtcgccg aggcgatgga
    11341 gtcccatatc ctggagcatg gcggtcggtt ggcgttcccc gtcaatatca gcctgaacga
    11401 taccgccgcc catttcactc ccaataccat cgatgacatg gtcttccaaa ggggcgacgt
    11461 tgtcaagctg gatgttgggg cccacgtgga tggctacctg ggggacacag ccaccaccgt
    11521 ggaggtgggc acccgcaatt ggcaaccgct catcagggct gccgagcgag cattggagat
    11581 ggccttggag atggccaagg acggcgtgac tgtgggcacc atcggcgggg tcgtggagag
    11641 aagcatcaaa gaggcgaacc tccgtcccgt cgtcaaccta agcggccacg agatgaagcg
    11701 gtacaacctc catgcagggc tctctatccc taacatcgac gacggaacgc tgaccaaggt
    11761 gcgggagaac atgctcctcg cgatcgaacc gttcgccacc aatggacagg ggcaggtatc
    11821 gaacgggcgg tcagggaata tataccgtgt attgagggac cggccattaa aggaccccga
    11881 cgcggagagg ctgttcgggc ggatcaagga ggagttcacc accctcccct tctgtgagcg
    11941 ctggtgcgac cggctgcagc ccaactccgg cccacttctg agggtacttc ttcgacacgg
    12001 cgtcatctcg acgtatccag tccttgtcga ggccaaacag gggatggtct cgcaggcgga
    12061 acacacggtg ctggtgggca atggccggaa cgagatcacc acggcgtgag ctcgaatacc
    12121 tttattaagg tcggctcttt tatccggcct cgcgcgaggg tagccaagct cggtcaatgg
    12181 cgcaatttgc gccgaccaaa ggcgacagac tcaagatctg ttcctgcaga ggttcgccgg
    12241 ttcgaatccg gtccctcgca ccagctcctt ccctccgacc caactcctca tatgtccttt
    12301 cgtacggtcg atggatgcca tggcgtaagg cctggcggtc aacgcccctc catgtccgtc
    12361 agcatatctc ggggaacatc gtcggggtcc ttaggctgga cggtcgtcct tttcatcgca
    12421 tcggccgatg ggatggcggt cacaggtatc ggtaccccat tgcacgatgg cgcatgctgg
    12481 acaatggtaa aagatgaaga aatggaagaa aaggaggaaa aacccctccc gtggttttta
    12541 cagcagccct atgataccga cgattatggc tatcacaccg atgatcgtct ggaacccacc
    12601 gagccatttg gcgagctttt ccagatgctt tcccaacgca gggatgctgg gcagaatgcc
    12661 cgttatgagg atcaatccgg cgatcaatgc caccacgctc tgtaggaggt cctcccacca
    12721 gatcaaggca agaataatag cgatgatgcc aatgatcgtc tggaacctcc ccaaccattt
    12781 ggacagcttc tcgaggtact tgcctagggc tgggatcgcg ggcaatatcc ctatcgccag
    12841 tattaacccc gccaagatgt taaccaatgc taaccaatcc attttcggtc acctctatat
    12901 aagatatttc ctctaaaatc aggtttatcg atattaataa ttatttacgc tatggctcga
    12961 aaatctggac gattattacg aaataatagc attttatata aggtcattct gcagcctacg
    13021 ctaagaacca gggtgtatgg gcacatatcg cacctgtatg gtcctctccg aactaaagga
    13081 acgatcggga acgattggta cgccatggat ttatatcccg ctgaccctct cccgttctga
    13141 cccaatggcc tttgccaagc cggtgaggca tcgggcaggg agggattaaa ctggcatcta
    13201 agaaatcaac gagcggtaag gccaagacat cgggtgggag gaagggaaac gaggatgaga
    13261 tggtggacga gcgtcccgcc gtcatgaaga agaaccccat ccgggccctt atgttcaagg
    13321 acgcactgct ggaggagatc gtggacggcc tcaaggcagg gcagatcccc aaggaggaca
    13381 tggacgaggc gctgttcctc acgactacca atgcgatcct cgagatcatc ggaatgaacc
    13441 tcccggagga ccttcagctc gtgttcgtgg aaaacctaga cgattacctg tccatcgcca
    13501 tgatcaacaa ggagcacgcc gtggacctgc tgggccaatt ccgggacggg ttcttggcat
    13561 cccatgagga cagcttcgag gacgagaaag cccttctgga ggccctcacc gattacgagg
    13621 aggagtggtg gggctcgccc aaggacttcc tgggcggggc ggtcccaaat gacctcatgg
    13681 aagaggcaag gaaaaggatg gacaccatcc tcgagcagga ggagggggct gaccaggaat
    13741 gcgaggggga agaggaggag gactactggg cctcccgttc ctatgtgatc cgcgacctgt
    13801 ggttcccctc cctgttggat agcattacca aagagcctct gccatccccg cagaggcggg
    13861 aggtgctgtt cacagccgcg gccaacgcca tcctcgacat agcggtgaac gtaatgccgg
    13921 atttcatgag cgaagagatg ttcgaagggc tggaccgcta cctggagctg actctggtga
    13981 acaaggaatg caaggtggac gtcctgacca tcttcgagga tgcgttggcc aagttcgaga
    14041 acgaatggtg gaccaccggc ctgaaggagc tcgg
//
LOCUS       DAOSYN010000016        13847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_3730_length_13847_cov_4.417996, whole genome shotgun sequence.
ACCESSION   DAOSYN010000016 DAOSYN010000000
VERSION     DAOSYN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 13847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13847
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3730_length_13847_cov_4.417996"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLJ11_01440"
     CDS             <1..236
                     /locus_tag="PLJ11_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOO03367.1"
                     /translation="GFDSPVFEGAHFNEFVVRTPVRPAKLNRLLLKRGIIGGLPLQRH
                     VPRMEDHMLLTATEATSAQAMDRLLAALREVAQ"
     gene            233..1687
                     /gene="gcvPB"
                     /locus_tag="PLJ11_01445"
     CDS             233..1687
                     /gene="gcvPB"
                     /locus_tag="PLJ11_01445"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOO03368.1"
                     /translation="MSYRQARYDEPLIFELRGESDFGLECDAPVRLPEGMERQGLDLP
                     DLPEREVVRHYVDLSQMSFGVDNGMYPLGSCTMKYNPKYADRLSSLPTVASVHPCQDE
                     DSIQGALRLMRELEKALCAISGMDAVTLQPAAGAHGEFTGMLIAKAYHARRGQPRTEV
                     IVPDSAHGTNPASAAMAGFTVIEVPSGPDGCIDLEALGAALSERTAAVMVTNPNTLGI
                     FEKDIVELARMVHAAGALLYYDGANLNAIMGMTSPGAMDFDIVHFNLHKTFATPHGGG
                     GPGAGPVGVKEFLEPFLPVPRIVSEEGRFGLDYDRPDSIGKVRSMFGNFAVLVRAYAY
                     ILVQGGDGLRDVSQRAVLASNYLKSRVVPPYEMPFAPLRKHEFVVSAKRLAKEKDVHA
                     TDVAKALLDRGFMSPTVYFPSLVEEALMIEPTESESKETLDRYAEALREIADGAPEEI
                     KAAPRSTSVARVDEVYAAKELILSWRIYRQRGLE"
     gene            1684..2289
                     /gene="tmk"
                     /locus_tag="PLJ11_01450"
     CDS             1684..2289
                     /gene="tmk"
                     /locus_tag="PLJ11_01450"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOO03369.1"
                     /translation="MSMEGAFIVLEGIDGVGKTTISKRLGAWLEGRGRKAVLTAEPTE
                     GWLGMAVRRANREDLDPRTEALLFTADRCQHTLAIERLLAEGNVVVSDRYYGSTVAYQ
                     GAALERGMGEGAVEWLLAMNRPVVRRPDLTVLLTCDPGAAMRRVGARGDRSKFEREGY
                     LGRVQEIYLMLAGDLGWTVVSSDGPLDEVFERVRRAVQAYV"
     gene            2314..3504
                     /gene="coaBC"
                     /locus_tag="PLJ11_01455"
     CDS             2314..3504
                     /gene="coaBC"
                     /locus_tag="PLJ11_01455"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOO03370.1"
                     /translation="MHPSEAITGTASDLLKGRTIVLGITGSIAAVESFDLARDLMRHG
                     ARVVPVLTPEAQRLVTPCSLAFATGQEPIVELDGRAQHVSLLGDVQGRADLLLIAPAT
                     ANTICKIACGIDDTPVTTMASVALGSGVPIVIAPAMHGAMFNNPMVAEAIERLKSVGV
                     GFVGPRMEGRKAKIADREETVHAVIRRLSQGLMKGRRVLVIGGSSAESIDDMRIVTNR
                     GTGETAVALALAAYHEGADTELWMGRCTVPLPAFLRIRRFEAVGDLLEMIKEVDHDLA
                     LVPAALSDFTFDRERGKLPSEAKATALQLKSVPKVLPSLKERCPKVIGFKAESGLGEE
                     GLIAEARASLKRYGLAAVAANDLKDVRPGSTRIVLVKARGRKVLEGSKADVARMLIAE
                     AARL"
     gene            3501..4361
                     /locus_tag="PLJ11_01460"
     CDS             3501..4361
                     /locus_tag="PLJ11_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate kinase"
                     /protein_id="HOO03371.1"
                     /translation="MRAKAFCPGHVTGLFQVCQDEDVMACGSRGAGLSLSLGVVSEVE
                     VVDGTGNVDIWLNGLKAAAPVTERAVRTILGDRPLDVRVRNELQLPVAQGFGTSAAGS
                     ISAAWALAHVLGVPEGEAYTAAHLAEIANRTGMGDVAGIMRGGIELRRRPGLPPIGEV
                     ERMNGHMDLVLAVVGPSIPTAEMLGRKEVLERINAVGGRGVELLMREPTLENLFRLSN
                     EFMEGSGLATPEVRRAVWEAKKWGWASQAMIGNSVFATGPDLDLLAGALSAYGDVYRC
                     SVDLKGPRLL"
     gene            complement(4442..4735)
                     /locus_tag="PLJ11_01465"
     CDS             complement(4442..4735)
                     /locus_tag="PLJ11_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HOO03372.1"
                     /translation="MRDTITKEHLDRYLALTKAALGKLRICAPERSFNRRLAESFLEM
                     ARTYFSDAEHFAAQGDYVNAFASVNYAHGWLDCGARVGLFDVGQDDQLFTLFE"
     gene            complement(4737..6317)
                     /locus_tag="PLJ11_01470"
     CDS             complement(4737..6317)
                     /locus_tag="PLJ11_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO03373.1"
                     /translation="MPAEEEVLEDAVEEVDSPDVDVAPRERRPSKHVLEDERYGRLDG
                     VGIIYGNVGATSFNLRVLGDLQKMDYVQVMHPSHGWVLGQVAEMERKTDLTLERAKQL
                     SEGMDVDIDEIVIAKISILGYRDERNLLQVPRSPFKAGGMVYHAKDELIKEVIGLKEN
                     TPTGAYLGKLFGYDIKVEVDINAMVQKHVSILAKTGGGKSFLCGDLVEELMKHDVTVL
                     ILDPHGEYGSMRDPGQVMPGKRDFGVQPMGFKDKIIEYATDTTLNKDAKPLKFTLANI
                     DARDLLALTSIKNGKIYLNALRKAIDGLRAVKKDYGLKEIIAMLESDEDTNNQTLIAE
                     LAYLNDVNIFAPQGTKIDELISQGKTTIINLKGTPPDIQELIVTRLATAMFELRKMGK
                     VPPMMMIAEEAHNFCPQQGVTACSKIFRTIAAEGRKFGLGLTIISQRAAKIDKNVLSQ
                     CNTQMILKVTNPNDLKAIVASVEGLQEGMDQEIQRLPIGVAVIMGGNVQMPLFVEVRP
                     RQSRHGGESVEIIPSKRL"
     gene            6504..7358
                     /locus_tag="PLJ11_01475"
     CDS             6504..7358
                     /locus_tag="PLJ11_01475"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOO03374.1"
                     /translation="MIVVGGSASTGLAKDLARELNAEYVQALTKTFPDGETYARIDRD
                     ALHGEVVIVQNSYPNDKLVELLLLQDAALGLGAEKVTCVIPYFGYARQDERFNRGEPL
                     SAKVMVEHIQMSADRVVLVDIHNPEIMDWFTKAKVKDVHAAPCIGDFFKEHGVDLVLA
                     PDEGALKRAGWTAERLGVDWDYMIKTRLSGTHVHMTPKNIDAKDRRVLIVDDIISTGG
                     TIIAATEELKRLGARNVMAACTHGLFVGNALDNLKKHVDKLACANTLESEVSLISVAP
                     VVARAIQE"
     gene            7423..8820
                     /gene="proS"
                     /locus_tag="PLJ11_01480"
     CDS             7423..8820
                     /gene="proS"
                     /locus_tag="PLJ11_01480"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOO03375.1"
                     /translation="MRKQEDFSEWYNEVVELANLTDKRYPIKGMNVWTPYGWRLMRLI
                     DTFIREECDATGHEEVCFPLLIPETEFKKEKEHIKGFDAEVYWVTHAGLNELDVRLLL
                     RPTSETAMYPIFALWVRSHTDLPLKVYQIVNTFRYETKQTRAFIRVREIHFFESHTCH
                     VDEADAQRQVEEDFVILENLMERLCLPYLLLRRTEWDKFPGAYYTVGVDTLLPDGRSL
                     QLGSIHHYRENFSRPFNITYEDEKGEHRFVHQTTFGMSERLVGAVVAVHGDDKGLVLP
                     PAISPFQVVIVPIPTKGNVERVAEECQRLRAELAAAGLRVKLDDGDERPGSKFNNWEI
                     RGVPLRLELGMRDIEGGKVAYARRDTGGKGALDRSAIVPQVHEVLRAIAVDMRARAAT
                     ASGAAIQDLDSLDRVPEKVVRFGWCGEEECGHRFEERTGLKMLGTPYLKEEYRGRCVV
                     CGREVDRAAYAARSM"
     gene            complement(8822..9697)
                     /locus_tag="PLJ11_01485"
     CDS             complement(8822..9697)
                     /locus_tag="PLJ11_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03376.1"
                     /translation="MPYLMGLLVAFEGVALIVLARRVELVDVMVMDRTIVMVLGLALF
                     ILGMVLFVPLFPQLQRAGERLMWRLQVGAAVTIIAISLLFLAMAAPIIIEGVGSIGKR
                     YAVLATAQLFFLGITALVFLFYEAAQDRRKAWLEWVGILASCLVMTEGVVIFGLRGDL
                     NIHGQLEAGPAFMTVIGIALVALGVIEVVVFNRRREGTERAIELLDWSGVAVSVVIGA
                     LGLFALTLVTSMTLDGVIYDYYWLLAAGLVPGLLGPLLDYTQTMVSDREGPTMDVGLV
                     TTIGLLIAIPIALLL"
     gene            complement(9777..9983)
                     /locus_tag="PLJ11_01490"
     CDS             complement(9777..9983)
                     /locus_tag="PLJ11_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03377.1"
                     /translation="MAEPAYPKRAVKMFSLLLIVAAALLYWVWGISYGSWNIFAAENM
                     GIYSLFVVLLGFGLFGYLLTKFKQ"
     gene            10294..10698
                     /locus_tag="PLJ11_01495"
     CDS             10294..10698
                     /locus_tag="PLJ11_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03378.1"
                     /translation="MPLDLWIPALVWLAIGAAIAIYLYRLMQREGKVEPLWVVIGLLL
                     SVLGLMAFIGVRSMRERGRDKAVDPKGYQPPEYKLKEGEGKEMVKTEPAPEPRPKKEV
                     KRIDGIPRCPECGAAVSSLDERCRECGAKLRK"
     gene            10736..11521
                     /locus_tag="PLJ11_01500"
     CDS             10736..11521
                     /locus_tag="PLJ11_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOO03379.1"
                     /translation="MLPEGSLVYLLDEKGRRTWVVLRKGMLRLGALGVVDGDRLLAAE
                     DGAELSIAGRRFWLLVPGASEMMSSVERGAQVITPKDAATILLELNIKAGDVVLEAGV
                     GSAALTIALLNQVRPWGKVVSMEIRPEFAENGRRNVEKAGLLEHWRLEIGDVRNDRLD
                     ILADAAVLDIPDPWNAVGNVAAMLRNGGRFCAYVPNTNQLEEVVRRLRREGFVEVRSL
                     ENIQREMVVHEMGVRPSYETLGHTGYLIFARKVERPGPAPIEH"
     gene            11609..12256
                     /locus_tag="PLJ11_01505"
     CDS             11609..12256
                     /locus_tag="PLJ11_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HOO03380.1"
                     /translation="MDLAFAVTAFASIFAVMNPVGNLPVFVAITEGYSPELKRKVRNK
                     VCIVAGSVLVVFALFGNVIFDLYGITIPAFKIAGGILLFSIAFTMTRGQLTKSKMTEE
                     EAEEASEKEEVGVVPLGIPLFAGPGAITTVMIYVSYAQDSGEPTLNLLFLFLGIFATV
                     AISFVLLKYAEPLFSRMGKSGAMAFTRIMGLLLAAMAVEFVLSGTFEAVSNHWGI"
     gene            12308..12547
                     /gene="purS"
                     /locus_tag="PLJ11_01510"
     CDS             12308..12547
                     /gene="purS"
                     /locus_tag="PLJ11_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOO03381.1"
                     /translation="MVVAEIRIELKPGVADPEGRNTRKALELLGFNDIRGVRSIKMFE
                     VELDADPAVARQECEEMCRRLLANPVIQKYRVDLR"
     gene            12553..>13847
                     /locus_tag="PLJ11_01515"
     CDS             12553..>13847
                     /locus_tag="PLJ11_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOO03382.1"
                     /translation="MSLEHLFIKRPVPFHLAEIDLAAATDDELKALSGEMGLSLSLEE
                     MRRVRDHFKGLGRRPTDVELESIAQAWSEHCCYKSSKVILREHIFGIDNGRVLSRGDA
                     GVMEFDDEYGYALRIESHNHPSAIEPYGGAATGIGGIVRDVLCMGAQPVALVDPLFFG
                     PLDLKGKVPAGSKAPKYLVSGVVSGIRDYGNRIGVPTVCGGLFFDESYLGNCLVNVGC
                     VGIVRKRDLRRNAVGGVGDRLILCGGRTGRDGIHGVTFASAELSARSEDESRGAVQLG
                     DPITKEPLIHACLEVNSLGLVSGMKDLGGGGLSCVVGEMALAGGCGAEVDLHRVPLKE
                     SGLAPWEVWVSESQERMMLAVPEGNVERVLETFAMWDVDATVIGKVVPGNHVTLEWLG
                     VRVLDLDLDFLTKGPEYCRPCKMEVRSRQTEEVAPKLPDL"
BASE COUNT         2462 a         4273 c         4757 g         2355 t
ORIGIN      
        1 aagggttcga ctcccccgtg ttcgagggcg cccatttcaa tgagttcgtg gtgaggacgc
       61 cggtgaggcc ggccaagctc aacaggcttc tcctgaagcg cggcatcata ggcgggctgc
      121 cgctgcagag gcacgtccca aggatggagg accacatgct cctgaccgcg acggaggcca
      181 cgagcgcgca ggccatggac cgcctcttgg ccgcgctgag ggaggtggcg caatgagcta
      241 ccgccaggcg cgctacgacg agccgctcat cttcgagctg aggggggaga gcgattttgg
      301 cctggaatgc gatgcccccg tacggctccc ggaagggatg gagcgccagg ggctggacct
      361 tcccgacctc cctgagaggg aggtcgtgcg gcactacgtg gacctgtccc agatgagctt
      421 cggcgtggac aacgggatgt accccctggg ctcgtgcacg atgaagtaca accccaagta
      481 cgcggaccgc ctctcctccc tccccacggt cgcatccgtg cacccctgcc aggacgagga
      541 ttcgatccag ggggcgttgc ggctgatgcg cgagcttgag aaggccctct gcgccatctc
      601 aggcatggat gcggtgaccc tgcagcccgc cgcaggcgcc catggggagt tcaccggcat
      661 gctgatcgcc aaggcatacc acgcccgaag gggacagccc cggacggagg tgatcgtccc
      721 cgactccgcc catgggacga acccggcgag cgcggccatg gccggattca ccgtgatcga
      781 ggtcccgtcc ggcccggacg ggtgcatcga cctggaggcc ctcggcgccg ccctctccga
      841 gcgcaccgcg gcggtgatgg tcaccaaccc caacaccctg ggcatctttg agaaggacat
      901 cgtcgagctg gcgaggatgg tgcacgccgc cggggcgctc ctctactatg acggggcgaa
      961 cctgaacgcc atcatgggga tgacctcccc cggggcgatg gacttcgata tcgtgcactt
     1021 caacctgcac aagacgttcg ccacccccca tggcggcggt ggcccggggg cagggccggt
     1081 cggcgtcaag gagttcctgg agccgttcct tccggtgccg cgcatcgtct ccgaggaggg
     1141 gaggttcggc ctggactacg accggccgga cagcatcggt aaggtgcggt ccatgttcgg
     1201 caacttcgcc gtgctcgtcc gggcatacgc atacatactg gtccagggcg gtgatggcct
     1261 gagggatgtc tcccagaggg ccgtgctcgc ctccaactat ctgaagagca gggtcgtccc
     1321 gccgtacgag atgcccttcg cccccctgcg caagcatgag ttcgtggtct cggccaagag
     1381 gctggccaag gagaaggacg tccacgccac ggatgtggcg aaggccttgc tcgacagggg
     1441 gttcatgtcc cccaccgtct acttcccctc cctggtcgag gaggccttga tgatagagcc
     1501 gacggagtcg gagagcaagg agaccctgga caggtacgcc gaggcgttac gggagattgc
     1561 cgacggcgcc cccgaggaga tcaaggccgc cccccgctcc acctccgtgg ccagggtgga
     1621 cgaggtctac gcggccaagg agctcatcct gagctggagg atatatcggc agaggggact
     1681 tgaatgagca tggaaggcgc cttcatcgtc ctcgagggca tagacggcgt ggggaagacc
     1741 acgatctcga agaggctcgg ggcatggctg gagggacggg gacggaaggc cgtcctcacg
     1801 gccgagccga ccgagggctg gctgggcatg gcggtccggc gcgccaaccg ggaggacctg
     1861 gacccgcgca cggaggccct gctcttcacc gcggaccgct gccagcacac ccttgccatc
     1921 gaacggctgc tggcggaggg gaatgtggtg gtgagcgatc gctactatgg gtcgacggtg
     1981 gcctaccagg gggcggccct ggagaggggg atgggagagg gcgccgtaga atggctcctg
     2041 gccatgaaca ggccggtggt gcggcgcccg gacctcacgg tgctcctcac gtgcgatccc
     2101 ggggcggcca tgcgaagggt gggcgcccgc ggcgatcgca gcaagttcga gcgcgaggga
     2161 tacctcggcc gagtacagga gatctacctc atgctggccg gggaccttgg gtggaccgtc
     2221 gtcagctccg atgggcccct ggacgaggtg ttcgaaaggg tgaggagggc cgttcaggca
     2281 tacgtttaag tccggccagt cggatttggc agcatgcatc cgtccgaggc cataacaggc
     2341 accgcctccg acctgctgaa ggggaggacg atcgtcctgg gcatcacagg ttccatcgcc
     2401 gccgtggaaa gcttcgacct ggccagggac ctgatgcggc atggcgcccg cgtcgtcccg
     2461 gtgctgacgc cggaggcgca gaggctggtg acgccctgct ccctggcctt cgccaccggc
     2521 caggagccga tagtggagct ggatggccgg gcgcagcacg tttccctcct gggggacgtt
     2581 cagggcaggg cggacctgct actcatcgcg ccggccacgg ccaacaccat ctgcaagatc
     2641 gcctgcggca tcgatgacac gcccgtgacg acgatggcct ctgtcgccct tggctccggc
     2701 gtgccgatcg tgatcgcccc cgcgatgcac ggggccatgt tcaacaatcc catggtggcc
     2761 gaggccatcg aaaggctaaa gagcgtcggc gtggggttcg tcggcccgcg gatggagggc
     2821 aggaaggcga agatcgccga ccgcgaggaa acggtgcatg cggtcatccg ccggctgtcc
     2881 caggggctga tgaagggcag gagggtgctc gtcatcggcg gctcctccgc ggagagcatt
     2941 gacgacatgc gcatcgtcac gaacaggggc acgggggaga cggccgtcgc cctggccctc
     3001 gccgcctacc acgagggggc ggacacggag ctttggatgg ggcgatgcac tgtcccctta
     3061 ccagcgttcc tccgcatcag gaggttcgag gccgtgggcg acctgctgga gatgatcaag
     3121 gaggtcgatc acgacctcgc tctcgtgccc gcggccctct ccgacttcac cttcgaccgg
     3181 gagcgaggga agctgccctc ggaggccaag gcgacggccc tgcagctcaa gagcgtgccg
     3241 aaggtgcttc catcgctcaa ggagcggtgc cccaaggtca tcgggttcaa ggccgagtcc
     3301 ggcctggggg aggaagggct gatcgcggag gcgcgggcct ccctcaaacg gtacgggctg
     3361 gcggcggtgg cggccaacga cctcaaggac gttcggcccg ggtccacccg catcgtgctg
     3421 gtgaaggcca ggggcaggaa ggtgctggag ggcagcaagg cggacgtggc ccgcatgctg
     3481 atcgcggagg ccgcgcgcct atgagggcca aggccttctg ccctgggcat gtcaccggtc
     3541 tcttccaggt ctgccaggac gaggacgtca tggcctgcgg ctcgcgcggg gcggggctga
     3601 gcctctccct gggggtggtg agcgaggtgg aggtggtcga tggaaccgga aatgtggaca
     3661 tctggctgaa cggcctgaag gccgccgccc cagtgaccga gcgggccgtg cgcaccatcc
     3721 tgggggatcg ccccttggac gtgagggtgc ggaacgagct gcagcttccg gtcgcgcagg
     3781 ggttcggcac cagcgccgcc ggctccatct ccgccgcctg ggccctcgcc catgtcctgg
     3841 gcgttccgga gggcgaggcg tacaccgccg cccatctggc ggagatcgcc aaccggacgg
     3901 ggatgggcga cgtcgccggc ataatgcggg gagggataga gctgcgccgg aggccggggc
     3961 ttccgcccat tggagaggtc gagcggatga acgggcacat ggacctggtg ctggcggtgg
     4021 tcgggccgtc gatccccacc gcggagatgc tcgggaggaa ggaggtcctg gaaaggatca
     4081 acgccgtcgg cggaaggggc gttgagctgc tcatgaggga gccgacgctg gagaacctct
     4141 tccgcctgtc gaacgagttc atggaggggt cgggcctggc gacgccggag gtgcggcggg
     4201 cggtctggga ggcgaagaaa tggggctggg ccagccaggc catgatcggc aattccgtct
     4261 tcgccaccgg gccggacctc gacctcctcg cgggggcgct ctcagcgtac ggtgatgtct
     4321 atcgctgctc ggtggatctc aaagggcccc ggctgctatg atgctggata gcgctgggca
     4381 agcggtgcgg gatatccctt ccctttcaat gagcgagacg gaccgaacgg gacgccggtg
     4441 atcactcaaa aagggtgaag agctggtcgt cctggcccac gtcgaagagg ccgacccggg
     4501 cgccgcagtc gagccagccg tgggcatagt tcacggaggc gaaggcgttc acgtaatcgc
     4561 cctgggcggc gaagtgctcc gcgtccgaga agtacgtccg ggccatctcc aggaaggact
     4621 cggcgagcct ccggttgaag gatcgctcag gggcgcagat gcgcagcttg cccagggcgg
     4681 ccttcgtgag cgcgaggtac cggtccagat gctccttcgt gatcgtatcc ctcatctcat
     4741 agcctcttgg aagggatgat ctccacgctc tctcccccgt gcctgctctg cctggggcgg
     4801 acctccacga acaggggcat ttggacgttc ccgcccatga tcaccgccac cccgatcggg
     4861 aggcgctgga tctcctggtc catgccctcc tgcagccctt cgacggaggc gacgatggcc
     4921 ttcaggtcgt tggggttggt gaccttgagg atcatctggg tgttgcactg ggagaggacg
     4981 ttcttgtcga tcttggccgc cctctgggag atgatcgtca gcccgaggcc gaacttgcgg
     5041 ccctccgccg cgatggtcct gaatatcttg gagcaggcgg tgacgccctg ctgcgggcag
     5101 aagttgtgcg cctcctcggc gatcatcatc atcgggggga ccttgcccat cttcctgagc
     5161 tcgaacatgg cggtggccag gcgggtgacg atgagctcct ggatgtctgg aggcgtgccc
     5221 ttcagattga tgatggtggt cttcccctgg gagatgagct cgtcgatctt ggtgccctgc
     5281 ggggcgaaga tgttcacatc gttcaggtac gccagctcgg cgatgagcgt ctggttgttg
     5341 gtatcctcgt cggattccag catggcgatg atctccttca gcccgtagtc cttcttgacc
     5401 gcccgcaggc cgtcgatggc cttgcgcagg gcgttcagat agatcttgcc gttcttgatg
     5461 gacgtgaggg ccagaaggtc ccgggcatcg atgttcgcca gggtgaactt gaggggcttg
     5521 gcgtccttgt tcaaggtcgt gtccgtggca tactcgatta tcttgtcctt gaagcccatg
     5581 ggctgcacgc cgaaatcgcg cttcccgggc atcacctggc cggggtcgcg catcgagccg
     5641 tactctccgt gcgggtcgag gatcagcacg gtgacgtcgt gcttcatgag ttcctccacc
     5701 aggtccccgc acaggaaact cttccccccg ccggtcttgg ccaggatgct cacatgcttc
     5761 tgcaccatgg cattgatgtc cacctcgacc ttgatgtcgt agccgaagag cttccccagg
     5821 tacgccccgg tgggggtgtt ctccttcagc ccgatgacct ccttgatgag ctcgtccttc
     5881 gcgtgataga ccatccctcc ggccttgaag ggcgagcgcg ggacctggag caggttccgt
     5941 tcgtccctgt agccaaggat ggatatcttg gcgatgacga tctcatctat gtccacgtcc
     6001 atgccctcgc tgagctgctt cgcgcgctcg agggtgaggt cggtcttgcg ctccatctcg
     6061 gctacctgcc cgagcaccca gccatgggac ggatgcatca cctgcacgta gtccatcttc
     6121 tgcaggtcgc ccaggaccct gaggttgaag gaggtcgccc ccacgttgcc gtagattatg
     6181 cccaccccgt ccaaacggcc atacctctca tcctccagaa catgcttgga gggcctgcgc
     6241 tccctggggg ccacatccac gtccggggag tcgacctcct cgaccgcgtc ctccaggacc
     6301 tcctcctccg cgggcatttc ctcttcaggg aggacggcct taccgctggg ctctgaaata
     6361 gaattggttt gcattcccat ccgtgctaga tatcgggacc tcccaataaa taggttgggg
     6421 atgaggttcg gcgcaacgtc ggcgcgtctg gaaggccgga cttgggagaa tcgcttatct
     6481 atcgggaact tgatctccgc ccgatgatag tcgtaggtgg ctccgcctcc actggtcttg
     6541 ccaaggacct cgccagagag ctgaacgccg agtacgtcca ggccctcacc aagacctttc
     6601 cggacgggga gacgtacgcc cgcatcgacc gcgacgccct ccacggggag gtggtcatcg
     6661 tgcagaactc ctatcccaac gacaagctcg tggagctcct gctcctgcag gacgccgccc
     6721 tgggcctggg cgcggagaag gtcacctgcg tcatccccta cttcggctat gccaggcagg
     6781 atgagcgctt caacaggggc gagcccctct ccgcaaaggt catggtggag catatccaga
     6841 tgagcgcgga cagggtcgtg ctcgtggaca tacacaaccc ggagatcatg gactggttca
     6901 ccaaggccaa ggtcaaggac gtgcacgccg ccccctgcat cggcgacttc ttcaaggaac
     6961 atggcgtgga cctcgtactg gcaccggacg agggggcgct gaagcgggcg ggatggaccg
     7021 ccgagaggct gggcgtggat tgggactaca tgataaagac ccggctctcc ggaacgcatg
     7081 tgcacatgac gcccaagaac atcgatgcca aggaccgcag ggtgctcatc gtcgatgaca
     7141 tcatctcgac cggcggaacg ataatagcgg ccaccgagga gctgaagcgg ctgggggcca
     7201 ggaacgtcat ggcggcctgc acccacgggc tcttcgtcgg caacgccctg gacaacctga
     7261 agaagcacgt ggacaagctg gcctgcgcca atacgttgga gagcgaggtc tccctgatct
     7321 ccgtggcgcc ggtcgtggcc agggcaatac aggaatgatg cctggcaaag gcttaaggcg
     7381 gaacggccca ttccgattcg atccggaccc aggggtgctg acatgaggaa gcaagaggac
     7441 ttcagtgaat ggtacaatga ggtcgtggag ctggccaacc tcaccgacaa gcgctacccg
     7501 ataaagggca tgaacgtctg gacgccgtac ggctggaggc tgatgcgcct catcgacacc
     7561 ttcatccgcg aggagtgcga cgccaccggt catgaggagg tatgcttccc cctgctcatc
     7621 ccggagacgg agttcaagaa ggagaaggag cacatcaagg ggttcgacgc tgaggtgtac
     7681 tgggtgacgc acgccggcct caatgagctg gatgtgcgcc tgctcctgag gccgaccagc
     7741 gagacggcca tgtacccgat cttcgcccta tgggtgcgct cgcacaccga cctcccgctc
     7801 aaggtctatc agatcgtgaa caccttccgc tacgagacca agcagacccg ggcgttcatc
     7861 cgggtgcgtg agatccattt ctttgaatcg cacacctgcc atgtggacga ggcggacgcc
     7921 cagcggcagg tggaggagga cttcgtcatc ctggagaacc tgatggagcg cctgtgcctt
     7981 ccgtacctgc tgctgcgcag gaccgaatgg gacaagttcc cgggggccta ctacaccgtg
     8041 ggcgtcgaca cccttcttcc ggacgggcgc agcctgcagc tcgggagcat ccatcattac
     8101 cgcgagaact tctcccggcc gttcaacatc acctatgagg acgagaaggg cgagcacagg
     8161 ttcgtgcatc agaccacctt cggcatgtcc gagaggctgg tcggcgccgt ggtcgccgtg
     8221 cacggggacg acaagggcct cgtgctccct ccggccatct ccccgttcca ggtggtcata
     8281 gtccctatcc cgaccaaggg caacgtggag cgggtggccg aggagtgcca gcggctgagg
     8341 gcggagctcg ccgccgctgg gctccgggtc aagctggacg acggggacga gcgccctggc
     8401 agcaagttca acaactggga gatccgcggc gtcccgcttc ggctcgagct ggggatgcgg
     8461 gacatcgagg gcgggaaggt cgcctatgcc cggagggaca cgggcgggaa gggcgcccta
     8521 gaccgctccg ccatcgtgcc ccaggtccac gaggtcctgc gggccatcgc cgtggacatg
     8581 cgcgcgaggg cggctaccgc ctccggcgcc gcgatccagg acctcgattc cttggacagg
     8641 gtccccgaga aggtggtgcg cttcggttgg tgcggggagg aggaatgcgg gcacaggttc
     8701 gaggagcgca ccggcctcaa gatgcttggc accccctatc tgaaggagga gtaccggggc
     8761 aggtgcgtgg tgtgcggaag ggaagtggac agggccgcgt acgcggcccg ctccatgtga
     8821 ttcaaaggag gagcgcgatc gggatggcga tgagcaaccc tatcgtggtg accagcccta
     8881 cgtccatggt cggcccctcc cggtcggaga ccatcgtctg cgtgtagtcc agcagggggc
     8941 cgagcagtcc agggacgagg ccagccgcca acagccagta gtaatcgtag atgacgccgt
     9001 cgagggtcat ggacgtgacc aaggtcaggg cgaataggcc cagggcgcca atgacgacac
     9061 tgacagccac cccgctccag tccaggagtt cgatcgccct ctccgtcccc tcccggcgcc
     9121 ggttgaagac cacgacctct atgaccccga gggcgaccag cgcgatgccg atgaccgtca
     9181 tgaaggccgg gcccgcctcc aattgtccat ggatgttcag gtccccccgg aggccgaaga
     9241 tgacgacccc ctccgtcatc acgaggcagg aggccaggat gccgacccat tccagccagg
     9301 ccttgcggcg gtcctgggcc gcctcataga acaggaagac cagggcggta atgcccagga
     9361 agaacagctg cgcggtggcg agcacagcat acctcttgcc tatggagccg acgccctcta
     9421 tgatgatggg cgcggccatc gccaggaaca gaagggagat ggcgatgatg gtcaccgcag
     9481 cccccacctg caacctccac atcaggcgtt ccccggccct ttggagctgc gggaagagcg
     9541 ggacgaagag gaccatccct agaatgaaga gggccaggcc gagcaccatc acgatggtgc
     9601 ggtccatgac cattacgtcc acgagctcca ccctacgggc cagaacgatg agggccaccc
     9661 cctcgaaggc cacgaggagg cccatcaggt atggcatgaa ccagctcccc aacttcctca
     9721 attgccccgc ctctgggcaa tattcagctg gaccggatat aatggttccg ttggggctac
     9781 tgcttgaact tggtgagcag gtagccgaac agcccgaagc ctagcaggac cacgaacagg
     9841 gaatagatgc ccatgttctc cgcggcgaaa atgttccagc tgccatagga gatcccccat
     9901 acccagtaga gcagcgccgc cgctacgatc aggagcagcg agaacatctt cacagccctt
     9961 ttggggtagg caggctcggc catcgggcgt caaacgtggt cggcctatta atatcttgtt
    10021 cccccagcca aacgctccct tccacaccga tgtcaggacg ggggcgggcc cggtgggcca
    10081 gccatttgga cgttttccgt ttagaggcgc cctccgcccc tcccctccgg ccggagctca
    10141 tgaatacagg gcggtcccca ggtcgcttcc cggccttagg gcccgatacc catcgccatg
    10201 gccctctgga cgatggggac gagggggttc cgagaggcaa aaatcaaaat cactttgata
    10261 tagcatgagg acgattgcga aggacgggac gggatgcctt tggacctctg gatacctgca
    10321 ttggtctggc tggcgatcgg cgcggccatc gccatctatc tgtatcggct catgcaacgg
    10381 gaggggaagg tggagcccct ttgggtggtg atcggcctcc tcctctccgt cctgggcctc
    10441 atggccttca tcggcgtccg ctcgatgagg gagaggggca gggacaaggc ggtggacccc
    10501 aagggctacc agcctccgga atataagctg aaggaggggg aggggaagga aatggtcaag
    10561 acggagcccg cccccgaacc gaggccaaag aaggaagtga agaggatcga cgggatcccc
    10621 cggtgcccgg agtgcggggc ggccgtctcc agcctggacg agcggtgccg ggagtgcggc
    10681 gccaagctaa ggaagtaggg gaaccctttt tatcccgcgg caggatgacc tacctatgct
    10741 gcccgagggc tccctggtct atcttttgga cgaaaaaggc aggaggacgt gggtggtcct
    10801 caggaagggc atgctgcggc tcggtgccct cggggtggtg gatggtgacc ggcttcttgc
    10861 ggccgaggac ggggcggagc tgagcatcgc tggcaggcgc ttctggcttc ttgtgccggg
    10921 cgcctcggag atgatgtcct ccgtggagcg cggagcgcag gtcatcaccc ccaaggacgc
    10981 cgcgaccatc ctcctggagc tgaatatcaa ggccggggac gtcgtgctcg aggcgggcgt
    11041 cggctccgcg gccctcacga tcgcgctgct gaaccaggtg cgtccctggg gcaaggtggt
    11101 ctccatggag atccgcccgg agttcgccga gaatgggcgg cgcaacgtgg agaaggccgg
    11161 cctgctggag cattggcgcc tggagatcgg ggatgtgcgc aatgaccgtc tggacatcct
    11221 ggcggacgct gcggtcctgg acatcccgga cccctggaac gccgtgggca acgtggcggc
    11281 catgctcagg aacggcgggc ggttctgcgc ctatgtcccc aacaccaacc agctggagga
    11341 ggtggtgcgc cgtctgcgca gggagggctt cgtggaggtc cgcagcctgg agaacatcca
    11401 gcgggagatg gtcgtgcacg agatgggcgt gcggccctcg tacgagacgt tgggccatac
    11461 cgggtacctc atcttcgcca ggaaggtgga gcggccgggc cccgctccca tcgagcactg
    11521 acccctggcc ggacagcggg ccatatgcca ttgggggcga gcgagcgccg agggcggggt
    11581 tatatatcca taggatggtt cctggcccat ggacctagcc tttgcggtga ccgccttcgc
    11641 ctccatcttc gcggtcatga accccgtggg caacctcccg gtgttcgtgg ccatcaccga
    11701 aggctattcg ccggagctga agcgcaaggt ccgcaacaag gtctgcatcg tcgccgggag
    11761 cgtcctggtc gtcttcgccc tcttcggcaa cgtcatcttc gacctgtacg ggatcacgat
    11821 cccggcgttc aagatcgccg gcggcatcct cctgttctcg atcgccttca ccatgaccag
    11881 ggggcagctg accaagtcca agatgaccga ggaggaggcc gaggaggcct cagagaagga
    11941 ggaggtgggg gtcgtgccgt tggggatccc cctcttcgcc ggcccggggg ccatcaccac
    12001 cgtcatgatc tacgtgagct atgcccagga ctccggggag cccaccctca acctcctctt
    12061 cctcttcctg ggcatcttcg ccaccgtggc gatatcgttc gtcctgctga agtacgccga
    12121 gccgctgttc tcccgcatgg ggaagagcgg ggccatggcc ttcaccagga tcatggggct
    12181 cctcctggcg gcgatggccg ttgagttcgt gctctcgggc accttcgagg cggtgtccaa
    12241 ccattggggc atctgaaagg gggcagaaga ttaattactg gaggatgatt cctcaggcgg
    12301 tgcatcaatg gtcgtggcag agatcaggat cgagctcaag ccgggggtcg ctgacccgga
    12361 gggcaggaac accaggaagg ccctggaact gctcggtttc aatgacatca ggggcgtgcg
    12421 ctccatcaag atgttcgagg tggagttgga cgccgacccg gccgtcgccc gccaggagtg
    12481 cgaggagatg tgccgcaggc tccttgccaa tccggtcatc cagaagtaca gggtcgacct
    12541 caggtgaagg gcatgtcctt ggagcatctc ttcatcaagc gcccagtgcc cttccatctg
    12601 gcggaaatag acctggcggc ggccacggac gacgagctga aggccctgag cggggagatg
    12661 gggctttccc tttcattgga ggagatgcgc cgggtcaggg accacttcaa ggggttggga
    12721 cggcggccga cggacgtgga gctggagtcg atagcccagg cctggagcga gcattgctgc
    12781 tacaagtcgt ccaaggtcat cctccgggag cacatcttcg gcatcgacaa cggccgcgtg
    12841 ctctcccggg gcgacgctgg ggtcatggag ttcgacgatg aatatgggta cgcgctcagg
    12901 atcgagagcc ataaccatcc atccgccatc gagccgtacg ggggcgccgc caccggcatc
    12961 gggggcatcg tccgggacgt gctgtgcatg ggcgcgcagc ccgtcgcgct cgtggacccc
    13021 ctcttcttcg gtcccctgga cctcaagggg aaggtccccg cgggctccaa ggcgcccaag
    13081 tacctcgtct ccggggtcgt ctccgggata cgtgactacg gcaaccgcat cggggtcccg
    13141 acggtgtgcg gagggctgtt cttcgacgaa tcatacctgg gcaattgcct tgtgaacgtc
    13201 gggtgcgtcg ggatcgtgag gaagcgggac cttcgccgca acgccgtggg cggggtgggc
    13261 gaccgcctga tactatgcgg ggggcggacc gggagggacg gcatccacgg cgtgaccttc
    13321 gcctccgcgg agttgagcgc caggtccgag gacgagagcc ggggggctgt gcagctcggc
    13381 gaccccatca ccaaggagcc gctcatccac gcctgcctgg aggtgaactc gctgggcctc
    13441 gtcagcggca tgaaggacct gggcgggggt gggctgtcct gcgtcgtggg cgagatggcc
    13501 ctggccgggg gctgcggcgc cgaggtcgac ctgcaccggg tgccgctcaa ggagagcggg
    13561 cttgccccct gggaggtctg ggtgtcggag tcgcaggagc gcatgatgct ggcagtgccc
    13621 gaggggaacg tggagcgggt gctggagacc tttgcgatgt gggacgtgga cgccacagtc
    13681 atcgggaagg tggtcccggg gaaccatgtc acgctcgaat ggctaggggt gcgggtcctc
    13741 gacctggacc tggacttcct gaccaagggg ccggagtact gccggccctg caagatggag
    13801 gtccgctcca ggcagaccga agaggtggcc cccaagctcc cggacct
//
LOCUS       DAOSYN010000017        13520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_3840_length_13520_cov_4.436539, whole genome shotgun sequence.
ACCESSION   DAOSYN010000017 DAOSYN010000000
VERSION     DAOSYN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 13520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13520
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3840_length_13520_cov_4.436539"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLJ11_01520"
     CDS             complement(<1..350)
                     /locus_tag="PLJ11_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO03383.1"
                     /translation="MTIRQPIVSVLGHVDHGKTSLLDYIRGTAVITREAGRITQHIGA
                     TEVPLEHINKVCAKLLGNKRFTIPGLLFIDTPGHHSFTTLRARGGSLADIAILVIDIN
                     EGLKPQTLESISIL"
     gene            complement(458..910)
                     /gene="ndk"
                     /locus_tag="PLJ11_01525"
     CDS             complement(458..910)
                     /gene="ndk"
                     /locus_tag="PLJ11_01525"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOO03384.1"
                     /translation="MMERTFVMLKPDAVQRGLIGQIVARFEAKGFRPVAMKFMRIPHE
                     LAERHYAEHRGKGFFPKLIEYMTSGPVLVMVWEGDNIISVVRGMMGKTNPAEAAPGTI
                     RGDYAQQTGRNLIHGSDSPESAKREIELFFNDYELLGYEACTKAWLFE"
     gene            complement(916..1266)
                     /locus_tag="PLJ11_01530"
     CDS             complement(916..1266)
                     /locus_tag="PLJ11_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HOO03385.1"
                     /translation="MVEQHLCSFCGEQIEPGTGKMYARKDGIVMLFCSNKCYKNMVEL
                     KRVPRNITWTKAYAAKKGVAKAAKERVEPKVEDKVEAAEEKVERKAEPKEKAGNRKKA
                     RAAKRAEAEAATKE"
     gene            complement(1280..1492)
                     /locus_tag="PLJ11_01535"
     CDS             complement(1280..1492)
                     /locus_tag="PLJ11_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HOO03386.1"
                     /translation="MADEESIPSEVVEIIGRTGMTGEATQVKVRVLDGRDKGRIITRN
                     IMGPVRMGDILMLRETSREARKLSLR"
     gene            complement(1505..1876)
                     /gene="rpl7ae"
                     /locus_tag="PLJ11_01540"
     CDS             complement(1505..1876)
                     /gene="rpl7ae"
                     /locus_tag="PLJ11_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HOO03387.1"
                     /translation="MAKAVFVRFETPKELKEKAYEVVELARDTGKLKKGTNEVTKIVE
                     RGEASLVVLAEDVQPPEILAHMPMLCDERNVPYVYVDSKAQLGNSCGLEKPTASIAIL
                     DAGKGKPILEALVDQVKKIKG"
     assembly_gap    2179..2278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2431..3183)
                     /locus_tag="PLJ11_01545"
     CDS             complement(2431..3183)
                     /locus_tag="PLJ11_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOO03388.1"
                     /translation="MPEPTEELIRKMDELRTDIKALLSSLNRADGQPYDGMASAFREQ
                     IGRSFLEQNRTAMRDAIGGFEGAPPRSQKALSQMTAQYEDAIDRFEKDDLNEALARLE
                     GLRESIVHSPEEILPAPMASTLLGVVERAREQMALAETLRFRVGRPMVRRSCEIALGG
                     VEPEDAERWLAPLASAPRLRIMAMLYTGPRPFTEMMKGLEMQKGHLRFHLNKLLEAGY
                     VKVEGRTHLYCIEERGYAAMKGLSGLLAQIRR"
     gene            3252..3881
                     /locus_tag="PLJ11_01550"
     CDS             3252..3881
                     /locus_tag="PLJ11_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOO03389.1"
                     /translation="MRIGVVACECFKNELEMVLAGETVEEKVYLEFGLHAYPQELRRE
                     IVRTVNGLKGKVDAVFLAYGVCNSLGGIADELEVPTVTLRADDCIGALLTPEEYARER
                     KRCAGTMFHIPFMSQMGVGYFEKELTSKMPNYKELGVDLDWYLGIMFDGYSRCLFIDT
                     GIGDRERYENLSKEFARQLSLRYETREGTVQMLRDGLMETKDLASRHLH"
     gene            complement(3878..4846)
                     /locus_tag="PLJ11_01555"
     CDS             complement(3878..4846)
                     /locus_tag="PLJ11_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO03390.1"
                     /translation="MSEMGTIKDMPANDMFVSGHGACPGCGMAVAVKNIMRILGADTT
                     VYVPASCLVVISAMYPTSSFRTPFLFTAFENTGAVTTGIRAAQRRRGLRSTVVGLAGD
                     GGTFDIGLQALSGAAERNEDILYVCLDNEGFMNTGIQRSGATPFGAWTTTTPVGKKVE
                     GKREFKKDIMAIVAAHDPPYAASLSIAHYNDFIKKVEKAKSKQGFRFLHVLTPCVPGW
                     RSDSSMTVEIARLAVETGMWTLYEVEDGNARTTYKPKAMLPVTDYLKLQGRFRHMEDA
                     DIAQLQEWLCAKWYLHYGDEIEQPVCAMLEKGKRMRYPHEEQLHFV"
     gene            complement(4843..6018)
                     /gene="porA"
                     /locus_tag="PLJ11_01560"
     CDS             complement(4843..6018)
                     /gene="porA"
                     /locus_tag="PLJ11_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOO03391.1"
                     /translation="MSVKVISGDHAVAYAAKLARTEVVPAFPITPQTLIVERLAEFIN
                     DGELDADFIPADSEHSAMSIAIGASAGGVRAFTATSSQGLALMHEMLYAVPQCRLPIV
                     MANVNRSLGAAAGIWVEHNDSMAERDSGWLQVYVEDNQEALDATLMAFRLAEDRRVLL
                     PVMVCLDGFILSHTVERVEVPGQEEVDRFLPRFEPCTVLDPAEPKLINPIVPPEYAME
                     MRYQLDRAVDRSREVIAEVDTGFGKAFGRSYGGLLDTYRLEDADFALLTLGTATGLAR
                     AAVDELRSEGKRAGLIKLRFMRPFPFQGLREKAEGLKALGVLDRSVSFNGGGPVYTEV
                     AAALCNVPTIVTGHVAGIGGRDIAPDHMREMFALVERAAKGERVRPVTWHGLRGEME"
     gene            complement(6015..6914)
                     /locus_tag="PLJ11_01565"
     CDS             complement(6015..6914)
                     /locus_tag="PLJ11_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO03392.1"
                     /translation="MYEVRFHGRGGQGAVMAAQTLAEAAVIEGMHAQAFPYFGAERRG
                     APVTAFARIDERKISIKSQVYEPDLLVVLDETLLDVEPVARGLKADGRAVINTRRLPT
                     EIDLGVEVECATVPATTIAMEHLKAPIVNTAILGAVVRMCALVSMESMREAIESRFGE
                     KFGEAAARTNVAAAAMAYQQAAVGRCKGERPLSSREEWLPTWQEVPIGVALGEGVKGG
                     VTVGPGGARRNLTGSWRVQTPRYDKGKCVRCLRCWFSCPEGCIKREEDDYVRWDLNYC
                     KGCGICAQVCPAKAIAIIKGGQG"
     gene            complement(7243..7494)
                     /locus_tag="PLJ11_01570"
     CDS             complement(7243..7494)
                     /locus_tag="PLJ11_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03393.1"
                     /translation="MGKTRKDLERDIRRWTPGNQRYLTHAKRAVADDLPERAKPFSAA
                     ADSETVNGMHPPGAMGGMTGSDAPSRPAIEGENAGHTNV"
     gene            complement(7568..8485)
                     /locus_tag="PLJ11_01575"
     CDS             complement(7568..8485)
                     /locus_tag="PLJ11_01575"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOO03394.1"
                     /translation="MKGKVWMDGRMVDFEDAKVHVLAHGLHYGTGVFEGIRVYHTSKG
                     RNIFRLRDHMVRFLNSAKVLQMDVPYSLDELCEACREVVRSAGPEGDYLRPLAFYGVH
                     PEWKIGLNPSGLGTNVSIMCTHMGVYMGEDQVEQGAAIITSSWEKYSNRAAALNAKVC
                     GHYVNSVIAKLEAIQRGCDEALLLNGNGSVAEGTGENVFMVKNGRLYTPPVSAGILEG
                     ITRDCVMTIAQDIGIEVTEKDLTRSELFMADEVFLTGTAAEISPVRSIDDRIIANGAT
                     GPMTQRLGEAFHRAAMGEDPRYKDWSDLV"
     gene            complement(8495..8887)
                     /locus_tag="PLJ11_01580"
     CDS             complement(8495..8887)
                     /locus_tag="PLJ11_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOO03395.1"
                     /translation="MQEEVRNGIEMEVISMVVNDEFGVMQRIMGEFTRRKINVDTIVV
                     GKCELPGKSRVVLTVSDRKQAEWAVDSLQRLHDILQVELVDESHQEAYALVGNGAGKA
                     RLIGSIEEVEKMLRATNPDRFIRAVNAI"
     gene            complement(8872..9102)
                     /locus_tag="PLJ11_01585"
     CDS             complement(8872..9102)
                     /locus_tag="PLJ11_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03396.1"
                     /translation="MVHSALLGLLVLGVAEGGVRRPKRIEGSDSHQLPWLSTPKYPCG
                     PDNGRADDGYYQPIKKEHSMRNYEIPIKCKRK"
     gene            complement(9387..9917)
                     /locus_tag="PLJ11_01590"
     CDS             complement(9387..9917)
                     /locus_tag="PLJ11_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO03397.1"
                     /translation="MVVDGTLDLENRRRIYELIRSRPGTYLREMERELGLQVGTLSYH
                     LGILTEAGMVRAEPEGNHVRYFPSEGFILNDRRTLAYLRSRTTRAILMHILDQGTVSF
                     QGLMALVKVSKSTMSYHLKRLASAGLIKVSKGEETMVSAADPVRLTNLLVWVREDVER
                     DAADALIDVWNRLIER"
     gene            complement(9917..10180)
                     /locus_tag="PLJ11_01595"
     CDS             complement(9917..10180)
                     /locus_tag="PLJ11_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03398.1"
                     /translation="MEAWQLALRIVLLGLSVIMLGISAQAWRRARGRRMVWVMVSFAG
                     FVLLSSSTLLGAIWGDSSWAVPDAIVGLLILIIGAIYLALLKG"
     gene            10330..11532
                     /locus_tag="PLJ11_01600"
     CDS             10330..11532
                     /locus_tag="PLJ11_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03399.1"
                     /translation="MRNAKALCLIVGGMLLLSAFALLAAPAVADGGNGRGHGPWSDGG
                     GETTIVPGQDDGMYEKGAKTSLMGKMNYSYGYGRGSFVFYTLDEANGTLTDYGLLTSG
                     GEKVLIANITVEGFDLQEIEVHGSIVRLSDGGLIAVIHDNPTGMYHLFVDEAANVSIV
                     LAGNMTVVENRVQNETSDLTYQLVIADGASRGVIASDDPFLVSENGTVVTCTVTEHLM
                     IRFLPQVAHRHQWIEMALMQAVREGRVAAEVTLVGGEEGGIYDTVSYRQELQVQVQEV
                     AKNRFQLRTQGQNAQGALVLVHTETGTMDMSQDRLRVRLNAQEMRCVEDPLELLYGQP
                     EDACYAVVDDGEVQQMLVYLPASTVGALTVESVDPMDALLSPGGVAIIIGAIALVVLA
                     GVVAFRKR"
     gene            complement(11554..12462)
                     /locus_tag="PLJ11_01605"
     CDS             complement(11554..12462)
                     /locus_tag="PLJ11_01605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO03400.1"
                     /translation="MLALSAGVCAYNEERNIVQCLRSLSAQRLNGKELVEILVISSGS
                     TDRTDELVARYAEEVDHRVRLIRQERREGKNSAVNAFIEAAKGEVLFLANADNVMRED
                     TFDLMAEHFLDPTVGVVGGHPVPVNDKGTFMGFAVHMLWDMHHRLSLIHPKVGEIVAF
                     RNTGLRIPVGMGSDEDLIRRDMEAKGLKVLYEPSAIVYNRGPTTLGDFLEQRTRVNIG
                     ERYMKRWFDYDIPTWDQRYLFNAYLSFLKDNARHPLKMAWAMALEAFARAYAALHVRL
                     DRGDRAIWQVVGSTKEVSADDKEKDR"
     gene            12578..>13520
                     /locus_tag="PLJ11_01610"
     CDS             12578..>13520
                     /locus_tag="PLJ11_01610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO03401.1"
                     /translation="MRSYEDLTVILPTYNEGGNIRSMLEVLIALYPGVSIIVADDNST
                     DGTPETVRSFSSVHPKTVLLARAPQDRGLTASIMDGIMACRTERFVVMDADFQHPPES
                     VGGLYDLLERGADVAVGKRVNKNSLSLSRTLASWGANALAKTYLFIMRKPSTEDVMSG
                     FFGGRTELCQDVLVKHGHRFERGGFKVLFDLLKFLPRNAVVEELEFEFHQRRSGHSKL
                     SSRVVLSILRQCGPMGKLAALAVNFLFINMLGRYISALALGLGFTFALMGTLNMAYDD
                     QLVTSTVLAFVLAMAYLVVANKFLLARGKRDRLIIGMK"
BASE COUNT         2302 a         4383 c         4042 g         2693 t
ORIGIN      
        1 ctgaggatac tgatcgattc caacgtctgg ggcttcagcc cctcgttgat gtcgatgacc
       61 aggatggcta tgtcggccag ggagcccccc ctggcgcgga gcgtcgtgaa ggagtgatgc
      121 ccgggggtgt cgatgaacag aagcccgggg atggtgaacc tcttgttgcc caggagcttg
      181 gcgcacacct tgttgatgtg ctccagcggc acctcggtgg ccccgatgtg ctgggttatg
      241 cgccccgcct cgcgggtgat gaccgccgtc cccctgatgt aatcgagcag gctggtcttc
      301 ccatggtcca catggcccaa cacactgact atgggttgac ggatggtcat gagcatcgga
      361 tttaggaatc tctattagta cctttcttgc aacggggagc acctgacatg cgggagcgcg
      421 gagaacgaga aaggagggtt ttgggagggg tctgtgccta ctcgaacagc caggccttgg
      481 tgcaggcctc atagcccagg agttcgtagt cattgaagaa cagttcgatc tcccttttgg
      541 ccgattccgg ggaatcggag ccatggatga ggttgcgccc ggtctgctgg gcatagtccc
      601 cgcgaatggt gccgggagcg gcctccgccg ggttggtctt gcccatcatc ccccgcacga
      661 cggagattat gttgtccccc tcccagacca tgacgagcac tgggccggag gtcatatact
      721 cgatgagctt tgggaagaac cccttgcccc tgtgctctgc gtagtgcctc tccgccagct
      781 catgtgggat gcgcatgaac ttcatggcca ctgggcggaa gcccttggcc tcgaagcggg
      841 cgactatctg gccgatgagg ccccgctgca cggcgtcggg cttgagcatt acgaaggtcc
      901 gctccatcat ggggctcact ccttcgtcgc cgcctcggcc tcggccctct tcgcagccct
      961 ggccttcttt cggttcccgg ccttttcctt gggctcggcc tttctctcga ccttctcctc
     1021 ggcggcctcg accttatctt cgaccttcgg ctcgaccctc tccttggcgg ccttggcgac
     1081 gcccttcttg gcggcatagg ccttggtcca ggtgatgttc ctgggcaccc tcttcagctc
     1141 caccatgttc ttgtagcact tgttgctgca gaagagcatg actatgccgt ccttcctcgc
     1201 gtacatcttg ccggtcccgg gctcgatctg ctctccgcag aaggagcata agtgttgctc
     1261 aaccatgtga acgacctcct tacctcaggg agagcttcct ggcctctctg gacgtctccc
     1321 tcagcatcag gatgtcgccc atacggaccg gccccatgat gttcctggtg atgatccttc
     1381 ccttgtcccg gccatccagc acacggacct tgacctgggt ggcctccccc gtcatgcccg
     1441 tcctgccgat tatctccacg acctctgaag gaatgctttc ttcgtcagcc atctgtgaca
     1501 ccctttagcc ttttatcttc ttgacctggt ccaccaaggc ctcgaggatc ggcttgccct
     1561 ttcccgcgtc gagaatggca atggacgcgg ttggcttctc caatccgcag gagttgccca
     1621 actgggcctt gctgtccacg tatacatagg gcacattcct ctcatcgcag agcatgggca
     1681 tgtgagccag gatctccggc ggctggacgt cctccgccag cacgaccagg gaggcctcgc
     1741 cgcgctccac gatcttggtg acctcgttgg tgcccttctt cagcttgccg gtgtctctgg
     1801 ccagctcgac gacctcatag gccttttcct tgagctcctt gggggtttcg aacctaacga
     1861 acactgcctt tgccaaactt atacctcctt ccgatgcgcg aacatcctca ttattcacgg
     1921 gctcataaga gcagggacgc tcaaagggat ggtcctataa aaggcttatc cgcccttcca
     1981 ttcaggaggg cgggcatccc cgaggccatc gccatgcctc aggccaggga ccaggtcgaa
     2041 agagggaaat atcgtctagt tgaatcgatg aacgatcgaa atgagcatgg cgaagaaggc
     2101 gatgtgcgta ataggggccg gaatggcctt catcggcaat gggctggcgt actatttcaa
     2161 caccgccacg catgaggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
     2281 aacaccgcca cgcatgagga gaccatcggg ccggtcgtat atgaggtcct gacctacgat
     2341 accgaccacg tcactggggc aatagcattg gccgtcatcg ggacgttcat actcctggtg
     2401 ggggccttgg ccaaggattg agcggtcgca tcaccgccgt atctgggcca ggaggccgct
     2461 gagccctttc atggcggcat accccctctc ctcgatgcag tacaggtgcg ttcggccctc
     2521 gaccttaaca taaccggcct cgaggagctt gttcagatgg aaccgcaggt gccccttctg
     2581 catctccagc cccttcatca tctccgtgaa ggggcgggga ccggtgtaca gcatggccat
     2641 gatcctcagg cggggggcgc tcgccaaggg cgccagccat cgctccgcat cctccggttc
     2701 cacccctccc agggcgatct cgcaggagcg gcggaccatg gggcggccga ccctgaaccg
     2761 cagcgtctcg gcgagggcca tctgctccct tgccctttcc acgacaccga gcaacgtgct
     2821 tgccatgggc gcgggcagga tctcctccgg cgaatggacg atgctttcgc gcaatccttc
     2881 caggcgggca agggcctcgt tcaggtcgtc cttctcaaag cgatcgatag cgtcctcgta
     2941 ttgggcggtc atttgggaga gtgccttctg tgatcggggc ggagccccct cgaagccccc
     3001 tattgcgtcc ctcatcgcgg tgcggttctg ctcgaggaag gaccttccga tctgctcccg
     3061 gaaggcggag gccatgccgt catagggttg cccatcggcc ctgttgaggg agctgagaag
     3121 ggccttgatg tccgttcgca gctcgtccat cttcctgatc agttcctcgg tgggctcggg
     3181 cattggtaat aactaattag tactaaataa tattttaata ttaccaatcc cgtgctcctt
     3241 ttggaattgg catgaggatc ggggtggtgg cgtgcgagtg cttcaagaac gagcttgaga
     3301 tggtgctcgc cggggagacc gtggaggaga aggtgtacct cgagttcggg ctacatgcct
     3361 acccccagga gctgaggagg gagatcgtca ggaccgtgaa cggcctgaag gggaaggtgg
     3421 acgccgtatt ccttgcgtat ggcgtatgca attcattggg cgggatcgcc gatgagctgg
     3481 aggtcccgac ggtcacgctc cgcgccgacg attgcatcgg cgccctgctc accccagagg
     3541 agtacgccag ggaacggaaa aggtgcgccg gcaccatgtt ccacatcccc ttcatgtccc
     3601 agatgggggt gggctacttc gagaaggaac tgacctccaa gatgcccaac tacaaggagc
     3661 tgggggtcga cctggactgg tacctgggca tcatgttcga cgggtactcc aggtgcctgt
     3721 tcatagacac cggcatcggg gaccgcgagc gctatgagaa cctctccaag gagttcgcgc
     3781 ggcagctgag cctgagatac gagacccggg aggggacggt gcagatgctc cgggacggcc
     3841 tgatggagac gaaggacttg gcatctcggc atctgcatta gacgaagtgc agctgctcct
     3901 cgtggggata ccgcatcctc ttcccttttt cgagcatcgc gcacacaggc tgctcgatct
     3961 catcgccata atggagatac cacttggcgc acagccattc ctgcagttgg gcgatatccg
     4021 cgtcctccat gtgcctgaag cgcccctgca gcttcagata gtcggtgacc ggaagcatgg
     4081 ccttcggctt gtacgtcgtc cgggcgttcc cgtcctccac ctcgtagagc gtccacatgc
     4141 ctgtctccac cgccagccgg gcgatctcca cggtcatgga cgaatcggag cgccagcctg
     4201 ggacgcaggg cgtcaggacg tgcaggaacc tgaagccctg cttgctcttg gccttctcga
     4261 ccttcttgat gaaatcgttg taatgggcga tggacagcga tgcggcgtag ggcggatcgt
     4321 gcgcggcgac gatcgccatg atgtccttct tgaactccct cttcccttcc accttcttgc
     4381 ccacgggggt ggtcgtcgtc catgccccga agggcgtggc gccggaccgt tggataccgg
     4441 tgttcatgaa gccctcgttg tccaggcaca cgtacaggat gtcctcgttc cgctccgcgg
     4501 cccccgacag ggcctggagg ccgatgtcga acgtcccccc gtccccggcc agcccgacca
     4561 cagtggagcg cagccccctc cggcgctggg cggccctgat gcccgtggta accgcccccg
     4621 tgttctcgaa ggcggtgaac aggaacgggg tgcggaagct gctggtgggg tacatggcgc
     4681 tgatgacgac gaggcaggag gcgggaacgt acacggtcgt gtccgcgccc aggatccgca
     4741 tgatgttctt gaccgccacg gccatcccgc acccggggca tgccccgtgg ccgctgacga
     4801 acatgtcgtt cgcgggcatg tccttgatcg tgcccatctc gctcattcca tctcccccct
     4861 gagcccgtgc caggtgaccg gcctgacccg ttcccccttc gccgcccttt ccacgagggc
     4921 gaacatctcc cgcatgtggt ccggggctat gtcccgcccg cctatgccgg ccacgtgccc
     4981 cgtgacgatg gtcgggacgt tgcatagcgc cgccgccacc tcggtatata caggcccgcc
     5041 gccgttgaac gacacggaac ggtcgaggac gccgagcgcc ttgaggccct cggccttctc
     5101 cctgagccct tggaacggga aggggcgcat gaagcgcagc ttgatcagcc ctgccctttt
     5161 cccctccgat cgaagctcgt ccaccgcggc ccgggccagc cccgtggctg tgcccagggt
     5221 gagcagggcg aagtcagcgt cctccaggcg ataggtgtcc aggaggccgc cgtaggagcg
     5281 gccaaaggcc ttgccgaacc cggtgtccac ctccgcgatg acctcgcggg aacggtccac
     5341 cgcccggtcc agctggtagc gcatctccat cgcgtactcg ggggggacga tggggttgat
     5401 cagcttgggc tcggcggggt caaggacggt gcatggctcg aacctgggga ggaagcggtc
     5461 cacctcctcc tgccccggca cctccacccg ctccacggta tgggagagga tgaagccatc
     5521 gaggcatacc atcaccggca ggagcacgcg cctgtcctcg gcaagccgga aggccatcag
     5581 cgtggcgtcc aaggcctcct ggttgtcctc cacgtatacc tggagccagc ccgagtcgcg
     5641 ctctgccatc gaatcgttgt gctccaccca gatgccggcc gcagcaccca gcgagcggtt
     5701 cacgttggcc atgacgatgg ggaggcggca ctgcggcacc gcgtacagca tctcatgcat
     5761 cagcgccagc ccctgggagg atgtggccgt gaacgcccgc actcccccgg cgctcgcccc
     5821 gatggcgatg ctcatggccg agtgctcgct gtccgccggg atgaagtccg cgtccagctc
     5881 cccgtcgttg atgaactcgg ccaaccgctc gacgatgagc gtctgcgggg tgatcggaaa
     5941 cgctgggacg acctcggtcc tagccagctt ggccgcgtag gcaacggcgt gatcgccgct
     6001 tatcaccttg acgctcatcc ctgtcccccc ttgataatgg cgatggcctt cgccgggcat
     6061 acctgcgcgc atatgccgca gcccttgcaa tagttgaggt cccaccggac gtaatcgtcc
     6121 tcctcccgct ttatgcaccc ttccgggcat gagaaccagc atcgcagaca gcgcacgcac
     6181 ttccccttat cgtaccgggg ggtctgcacc ctccagctgc cggtgaggtt cctccgcgcc
     6241 ccgcccgggc ccaccgtcac cccgccctta acgccctcgc cgagggcgac cccgatgggg
     6301 acctcctgcc atgtgggcag ccactcctct ctgctcgata gtggcctttc ccccttgcac
     6361 ctgccaacgg cggcctgctg gtaggccatg gcggccgcgg ccacgttcgt gcgggcggcg
     6421 gcctccccga acttctcccc gaaacggctc tcgatggcct cccgcatgga ttccatgctc
     6481 accagggcgc acatccggac caccgcgccg aggatggccg tgttgacgat gggcgccttc
     6541 aggtgctcca tggcgatggt cgtggcgggc accgtggcgc actccacctc cacccccagg
     6601 tcgatctcgg tgggcagccg gcgggtgttg atcaccgccc tcccatcggc cttcagcccc
     6661 ctggccaccg gctctacgtc cagaagggtc tcgtcgagca ccaccaacag gtccggctcg
     6721 tagacctggg acttgatgct gatcttgcgc tcatcgatcc gggcgaaggc cgtgaccggc
     6781 gccccccggc gctccgcccc gaagtacggg aacgcctgcg cgtgcatccc ttcgatcacc
     6841 gccgcctcgg ccaatgtctg ggcggccatt acggccccct gtccccctcg tccatggaac
     6901 cggacctcgt acaacgcaat ccccatatga gagaggccag cggccgatat taaatcctac
     6961 tggaagaaat ggtcgggaag gggttgcagg gggccatgcc gtcctcttga acgttcgttc
     7021 ttggatactt cgcccctgcc atctttccag cgggccaccg cccctctgca cctccccgca
     7081 gacctggcgc atccggagcc gttccctggg cgggtccctc ctcgcctgca tggcctccag
     7141 ggcagccctg cggagggtct tgtggccctc ccccttggcg tggtgcaagc cggtcctggc
     7201 cttttgctcg ccctccgcct tggcctgcac gatcgttggc ggctacacat tggtatgccc
     7261 ggcgttctcg ccctcaatgg ccggccttga gggtgcatcg gagccggtca tcccgcccat
     7321 cgcccccggg gggtgcatgc cattcactgt ttccgagtcc gcggcggcgc tgaacggctt
     7381 ggccctctcc ggaaggtcgt ccgccacggc ccgcttggca tgggtcagat atctttgatt
     7441 gcctggggtc caccggcgaa tatccctttc aaggtccttc ctggtcttgc ccatcgtcct
     7501 tccccttggt gatcgatgga catggacgtg gcctgggaag gccacgcatc ccctggttcc
     7561 aacggggcta caccaggtcg gaccaatcct tgtacctggg gtcctccccc atggccgccc
     7621 tgtggaacgc ctcccccagc cgctgggtca tcggccctgt cgccccgttt gcgatgatcc
     7681 ggtcgtcgat ggagcggacc ggggagatct ccgccgcggt gccggtcagg aacacctcgt
     7741 ccgccatgaa gagttcggag cgggtcagat ccttctccgt cacctcgatg ccaatatcct
     7801 gggcgatggt catgacgcag tcacgggtga tgccctcgag gatgccggcg gacaccgggg
     7861 gggtgtacag ccttccgttc ttgaccatga acacgttctc ccccgtcccc tcggcgaccg
     7921 agccgttccc gttgagcagg agcgcctcgt cgcagcctcg ctggatcgcc tcaagcttgg
     7981 ctatcactga attgacgtag tgcccgcaca ccttggcgtt gagggcggcg gcgcggttgg
     8041 agtacttctc ccaagaggat gtgatgatgg ccgccccctg ctccacctgg tcctctccca
     8101 tgtagacacc catgtgggtg cacatgatgc tcacgttggt gcctagtccg cttgggttca
     8161 gtccgatctt ccactcggga tgcaccccat agaaggccaa cggcctcaag tagtcccctt
     8221 ctggcccggc ggagcggacc acctcccggc aggcctcgca cagctcgtcc aatgaatacg
     8281 ggacgtccat ctgcaatacc ttggcggagt tcaggaagcg aaccatgtgg tcccgcagac
     8341 ggaagatgtt cctccccttc gaggtgtggt agacacggat cccctcgaac accccggtcc
     8401 catagtgaag cccgtgggcc agcacgtgca ccttcgcgtc ctcgaagtcc accatgcgcc
     8461 cgtccatcca gaccttgccc ttcatggccg cacctcagat ggcgttcaca gccctgatga
     8521 agcggtccgg attggtggca cggagcatct tctccacctc ctcgatggag ccgatcagcc
     8581 gagccttacc ggcgccgttg cccaccagcg catacgcttc ctggtggctc tcgtccacta
     8641 gctccacctg caggatgtcg tgcagccgtt gcaggctgtc caccgcccat tccgcctgct
     8701 tgcggtcgga gacggtcagg accacgcggg acttgccagg cagctcgcac ttcccgacaa
     8761 cgatggtgtc cacgtttatc ttgcggcggg tgaactcgcc catgatcctc tgcatgaccc
     8821 cgaactcgtc gttcacgacc atcgatatga cctccatctc gatcccgttc ctcacttcct
     8881 cttgcacttt atcggtatct cgtagttccg catgctgtgc tctttcttga tcggttgata
     8941 gtacccgtcg tcggcgcgcc cgttgtccgg accgcagggg tacttgggcg tggacagcca
     9001 gggaagctga tggctatcgc ttccctctat tctcttcgga cggcggaccc caccctctgc
     9061 cacacctagc accaacagcc cgagcaacgc gctatgcacc attaacatac ccaccatatg
     9121 gacggacagg ccattggcct ccctttcaga cgcccaatgc acgcaggacc tgaacaataa
     9181 caccggggag ggccgcagcg cgcatggaga ttttgtattt ctgctaccgt atttaagggt
     9241 tgtcttaggc cctgccgtca ttcagtaatg accggcggcc cgctccggac catggctgga
     9301 tggacgggga ggccccatga ttgcattggc ggcgctcgga cccatgccct ttctcccctc
     9361 ccaacggagc ggggcccgat ggaaccctat ctctcaatga gcctgttcca aacatcgatg
     9421 agggcgtccg cagcgtccct ctccacatcc tcccggaccc agaccagcag gttcgttagg
     9481 cggacggggt cggccgcgga gaccatggtc tcctcgccct tgctcacctt gatcagcccg
     9541 gcggaggcca ggcgcttgag gtggtaggac atggtcgact tcgatacctt gacgagcgcc
     9601 atcagcccct ggaagctcac cgtcccctgg tcgaggatgt gcatgaggat cgccctagtg
     9661 gtccgggagc ggaggtatgc cagtgtccgc cggtcgttga ggatgaagcc ctccgagggg
     9721 aagtaccgga catggttccc ttccggctcg gcccgcacca tgcccgcctc ggtgagtatc
     9781 ccgaggtgat aggagagcgt tccgacctgc agccccagct ccctctccat ctcccgcagg
     9841 tacgtccctg gcctggaacg gatcagctcg tatattctcc gtcggttctc caggtccagg
     9901 gtgccgtcca ccaccatcag cccttcagca gcgccaggta aatggcgcct attatcagga
     9961 tcagtagtcc aacgatcgca tcgggaacgg cccatgagga atccccccat attgccccca
    10021 ggagggtgga cgaggaaagg aggacgaacc cggcgaagga gaccatcacc cacaccatcc
    10081 gcctccctct ggcccgccgc caggcctggg cgctgatgcc cagcattatc acgctcaggc
    10141 cgagcagtac gatcctcagg gccaactgcc acgcttccat gcgctcgaaa tcgtcgggca
    10201 ggggataagc atcttctggt tcaatcatcg aaccgccgtc cgggggcgtt cgaagcttgt
    10261 accagccggt tcgagggacg taccaaaacc ctaatgaccc ctccccccgg atgcgagagc
    10321 ggtgagaaca tgcggaacgc aaaggcattg tgcctgatag tagggggcat gctccttctc
    10381 tccgccttcg cattgctagc ggcgccggcc gtggccgacg gtggcaacgg gcgggggcat
    10441 ggtccctggt ccgacggcgg tggggagaca acgatcgtgc ctggacagga cgatgggatg
    10501 tacgagaaag gagcgaagac atccctgatg gggaagatga actactcgta tggctatggc
    10561 agagggagct tcgtcttcta cacattggat gaagcgaacg gcaccctcac cgactacggc
    10621 ctgctgacct ccggggggga gaaggtcctg atcgctaata tcacggttga agggttcgac
    10681 cttcaggaga tcgaggtgca cgggtccatc gtcaggctga gcgatggggg tctgatcgcc
    10741 gtcatccacg ataatcctac gggcatgtac catctgttcg tcgacgaggc cgcgaacgtg
    10801 agcatcgtcc tggctgggaa catgaccgtg gtggagaaca gggttcagaa cgaaacgagc
    10861 gatctgacct accagctggt catcgccgat ggggcctcga ggggcgtcat cgcctccgac
    10921 gaccccttcc tggtgtcgga gaacggcacg gtggtcacat gcaccgtcac ggagcatctc
    10981 atgatacgct tcctgcccca ggtggcccat cgtcatcaat ggatcgagat ggccctgatg
    11041 caggccgtcc gtgagggcag ggtggcggcc gaggtcaccc tcgttggcgg ggaggagggc
    11101 ggcatctacg acaccgtttc ctaccgccag gagctgcagg tgcaggtgca ggaggtggcc
    11161 aagaacaggt tccaactgag gacccagggc cagaacgccc agggtgccct cgttctggtc
    11221 cacacggaga ctgggaccat ggacatgtcc caggaccgcc tccgggtgcg cctgaacgcc
    11281 caggagatgc gctgcgtcga ggacccgcta gagctcctgt acgggcaacc tgaggacgcc
    11341 tgctacgcgg tcgtggacga tggggaggtc cagcagatgc tggtctacct gcccgcaagc
    11401 accgttggcg cgctcacggt cgagagcgtc gatcccatgg acgcgctgct ctcccccggc
    11461 ggcgtggcca ttatcattgg ggccatcgca ttggtggtcc tggcgggcgt ggtggctttc
    11521 aggaagcgat gagcggcccc ctcaaacatt ttcctatcta tctttctcct tgtcatcggc
    11581 gctcacctcc ttggtgctgc ccaccacctg ccagatcgct cggtcccccc tgtcgagacg
    11641 gacgtgcagt gcggcgtagg ccctggcgaa ggcctcgagg gccatggccc aggccatctt
    11701 aagcgggtgc ctggcattgt ccttgaggaa gctgaggtag gcgttgaaga ggtatcgctg
    11761 gtcccaggtc gggatgtcgt agtcgaacca ccgcttcatg tagcgctccc cgatgttcac
    11821 ccgggtgcgc tgctcgagga agtcccccag ggtggtcggc ccccggttgt agacgatggc
    11881 cgaaggctcg tacaggacct tgagcccctt ggcctccatg tcccttcgga tgaggtcctc
    11941 gtcgctcccc atccccaccg ggatcctcag ccccgtgttc cggaaggcca cgatctcgcc
    12001 caccttcgga tggatgagcg agagtcggtg gtgcatgtcc catagcatgt gcaccgcgaa
    12061 gcccatgaag gtgcccttgt cgttcaccgg aacaggatgc ccgccgacga cccccacggt
    12121 cggatcgagg aagtgctccg ccatgaggtc gaaggtatcc tctcgcataa cgttgtccgc
    12181 gttggccagg aaaaggacct cccctttggc tgcctcgatg aacgcgttca cggcggagtt
    12241 cttgccctcc cgcctttcct gtctgatcag cctgacccga tggtcgacct cctccgcata
    12301 gcgggccacg agctcgtctg tacggtccgt gctgccgctc gagatgacaa gtatctccac
    12361 gagctccttc ccattgagcc tctgggcact taaggaacgg aggcactgga cgatgttcct
    12421 ttcctcgttg tacgcgcaca ccccggcgga gagggcaagc atcaaagagg agaacgggaa
    12481 ggtgtgataa atattgtcga ggccgttggt cggcgttctc cggtcaacaa tctataaata
    12541 tcaataaaaa atcttgagtt gcggagttgg gatgctattg cggtcctacg aagacctgac
    12601 ggtgatcctt cccacataca atgagggagg gaacatccgt tccatgcttg aggtcctcat
    12661 tgcgttgtac cccggggtct cgatcatcgt cgccgatgac aactccaccg atggtacgcc
    12721 cgagacggtg cgctcgttct cgtcggtcca tccgaagacc gtgctcctcg cccgtgcccc
    12781 ccaggacagg gggctgaccg ccagcatcat ggatggcatc atggcctgcc gcaccgagcg
    12841 gtttgtggtc atggatgccg atttccagca tcccccggaa tcggtgggag ggctgtatga
    12901 cctgttggaa cggggggcgg acgtcgcggt cggcaagcgg gtgaacaaga acagcctgag
    12961 cctcagccgg acgctggcct catggggggc gaacgccttg gccaaaacgt acctgttcat
    13021 aatgcgcaag ccctccaccg aggacgtgat gagcggcttc ttcggaggga ggaccgagct
    13081 ctgccaggac gtactggtga agcatgggca taggttcgag cggggcgggt tcaaggtcct
    13141 cttcgacctg ctgaagttcc ttccccggaa tgcggtcgtc gaggagctgg agttcgagtt
    13201 ccaccagcgc cgcagcggtc attccaagct ttcctcgcgg gtcgtcctgt ccatcctcag
    13261 gcagtgcggg ccgatgggga agctggccgc attggccgtg aacttccttt tcatcaacat
    13321 gctgggaagg tacatctccg ccttggccct cggcctcggc ttcaccttcg ccctgatggg
    13381 caccctgaac atggcctacg atgaccagct cgtgacctcc acggtcctgg cgttcgtcct
    13441 ggccatggcc tatctcgtgg tggccaacaa gttcctgctg gcccgtggga aaagggaccg
    13501 cctgatcatt ggcatgaagg
//
LOCUS       DAOSYN010000018        13505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_3848_length_13505_cov_4.184535, whole genome shotgun sequence.
ACCESSION   DAOSYN010000018 DAOSYN010000000
VERSION     DAOSYN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 13505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13505
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3848_length_13505_cov_4.184535"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLJ11_01615"
     CDS             complement(<1..435)
                     /locus_tag="PLJ11_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOO03402.1"
                     /translation="MRRDVLVVGGGPTGCLAAAAMAAELEVTVVEEHPRIGEPVQCAG
                     LVTPRVVEMASAPDAVLNRIDGAYVHFPGGRTLELQGNEVKAVVVDRGALDRRCASLA
                     ERAGAEVWTGVRCGAVERTGDGIRACFDGEDMECRALLAADGY"
     gene            complement(505..1023)
                     /gene="fsa"
                     /locus_tag="PLJ11_01620"
     CDS             complement(505..1023)
                     /gene="fsa"
                     /locus_tag="PLJ11_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOO03403.1"
                     /translation="MNEICEVVDGPISVEVVSLRAEEMFAEGMRLAAVNDNIVIKVPM
                     TEEGLKATKMLSNEGVSVNVTLIFSANQALLAAKAGARYVSPFVGRLDDVGQDGMALV
                     SEIMDILDNYDFDTEVIVASVRHPIHVAEAARMGAHIATIPYDVLKKMFKHPLTDIGI
                     ERFLKDWEKVPK"
     gene            complement(1007..1147)
                     /locus_tag="PLJ11_01625"
     CDS             complement(1007..1147)
                     /locus_tag="PLJ11_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03404.1"
                     /translation="MKIFIDTANIEQIREVSSWGILNGVHEPQPDSQGEEGPRHHRER
                     DL"
     gene            complement(1144..2088)
                     /locus_tag="PLJ11_01630"
     CDS             complement(1144..2088)
                     /locus_tag="PLJ11_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOO03405.1"
                     /translation="MSWPMEAQRKSYGRALVDLGRARGDVVVLDADLSSSTRTVEFKG
                     AFPGRFFNCGIAEQNMMGTAAGLAVGGMTVFASTFAVFATGRAYDQVRQSIAYPDLNV
                     KIVASHAGITVGGDGASHQTMEDIALMNALPNMTVVVPADGPETYKAVLAVAERHGPA
                     YVRMGRGDVPVVTSKEERFEIGRAHTLREGADVALVGTGQMVAACLLAAEALKARGVS
                     AKVIDLHTIKPLDVEALEKAARGCGAVVTAEEHNIVSGVGTLVAAALAERYPVPMERV
                     AMQDVFGQSGESEELMREYGLTDREVLTAAIKAMERKK"
     gene            complement(2085..2921)
                     /locus_tag="PLJ11_01635"
     CDS             complement(2085..2921)
                     /locus_tag="PLJ11_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOO03406.1"
                     /translation="MNSPDQISVSELEAKALALRRHVIRMIYAAGSGHPGGSLSCADV
                     IAALFFKVMDHSPERMGSQDRDHFILSKGHAAPVYYAALAECGYFPTEELLTLRKLHS
                     RLQGHPSRVKTPGVEMSTGSLGQGLSVANGLALALRLLGLDRKVYCLCGDGEMAEGQV
                     WEAAMFAAHYHLDNVIAIVDRNGLQIDGRTEEVMSLEPLGDKWRAFGWHVLFMDGNDM
                     WQVLDTLAEAKGHKGSPTVIIARTVKGKGVSFMENNVGFHGKAPNAKEYEQAMRELGG
                     EV"
     gene            3100..3873
                     /locus_tag="PLJ11_01640"
     CDS             3100..3873
                     /locus_tag="PLJ11_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO03407.1"
                     /translation="MVAIEVPCLANERLKAVLERINADEELRALWKASNINAIDRLGY
                     SDHGSTHVRIVARNALRMLRLLMEAGHRPGAVLNYGMRPEDAEVVVVLASALHDIGLS
                     VHRQNHEEFSVMLSAPILRRLLGNVYQEPELTFVVAEVQHAMISHHEGFAPYTIEGGV
                     VRVADALDMEKGRARIPFASGSVNIHSVSALAIDKVRVGKGKERPILIDITMNNSAGI
                     YQVDELLKEKIARSGLRELITVEVKVPEAESRIVKELRL"
     gene            complement(3870..4607)
                     /gene="pcn"
                     /locus_tag="PLJ11_01645"
     CDS             complement(3870..4607)
                     /gene="pcn"
                     /locus_tag="PLJ11_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030337 - DNA polymerase processivity
                     factor activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proliferating cell nuclear antigen (pcna)"
                     /protein_id="HOO03408.1"
                     /translation="MFQAKLRSDTLKGVVDVVSTLIDEAKFKIGENGIQLKAVDPAHV
                     AMVDLNLSKEAFEQYSATETDLGLDLEKLKEVIRLSRSGDIIDMRHDVERGRLVITVG
                     NVTRRMNLVSTEGMSDPKVPNITLPARITIAVDELQRGIKAAESISDHVALNANAEGF
                     EMVSDGDTDSVSLKLPKALLVDLQCSESIRSLFPMDYFSTIIKAIPSGTNVTINLGND
                     LPVRLDFDIAGGKGSVRYLLAPRIEND"
     gene            complement(4671..4976)
                     /locus_tag="PLJ11_01650"
     CDS             complement(4671..4976)
                     /locus_tag="PLJ11_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HOO03409.1"
                     /translation="MFCPKCHKIMKPDNGKFVCRNPDCKHEVIKNGEPVCFTTSGKEK
                     EIAVIDGNAPTLPRTRIDCPKCGYQEAFWVLRQTRAADEPETKIYRCVSCGHSWREY"
     gene            complement(5033..5935)
                     /locus_tag="PLJ11_01655"
     CDS             complement(5033..5935)
                     /locus_tag="PLJ11_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03410.1"
                     /translation="MGFRRSADADDEITNTLIDRGVYRLSEVYEDEEIKRYRDVKVPY
                     SFWSAVRYSFILTVLLWWLPLLGQMVAGFVSGRRAGKPWKGVLAALVPLTVIFTITLL
                     VDSGTIPTVINGVDWTPSHLLAWVERAVPVLAPYIVFVQMYMTSLIDAIQISAGVHVD
                     VYIITAAFAYIGGILAEQSQLEMGYVAKHGGNSMTVVVSNGERGKGEPEASRPAVGSR
                     PVSARPIRTRQEQRVIRFSDLEDLTTASDDGYAEEYSEKGEGWYEEGEEPEEEIPRNM
                     AGLAKRSKGGGKGKNLKPGDWKFI"
     gene            complement(6026..6382)
                     /locus_tag="PLJ11_01660"
     CDS             complement(6026..6382)
                     /locus_tag="PLJ11_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03411.1"
                     /translation="MAEFAMGYGGGAPLCTDRVWISSAFRVRLIKMGMEKAGSVNELG
                     RRLGYRSRVHPGWGVVQIMQGKQAFPLARLKLLADFLEYPIEEIMRYATQPNRVTPES
                     TKSALAMYGLSGYIPR"
     assembly_gap    6573..6672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6691..7083
                     /locus_tag="PLJ11_01665"
     CDS             6691..7083
                     /locus_tag="PLJ11_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOO03412.1"
                     /translation="METQGEGDLVDVTSLVERTVTGSGLRQGIALVFTPHSTAAVVTL
                     ENEPGLREDLFQALERNFPKGSEYQHHLRWGDGNGHSHIRSTFLGASVALPFDGGRLE
                     RGEWQQVALLELDVRGRRREVIVQLLGE"
     gene            7120..8358
                     /locus_tag="PLJ11_01670"
     CDS             7120..8358
                     /locus_tag="PLJ11_01670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO03413.1"
                     /translation="MKVKFLGGAEIVGKLGMLVENNGASIMFEYGLRLIKKEPPQFPM
                     ESPRVEGVFVTHSHLDHSGAVPRLVQDTDCDVYCNDLTMDVASLLFFDSIKIAKSEGY
                     ENILRYNDGDVKHVWQNYRSFDVGDVKPIAGLEIVPHNAGHIPGAQMYEVKGHDTTLF
                     TGDLHTANTRLTYGAKPVECDNLVIEATYAGRNHPPREKSEALLQQKVREVVERGGTA
                     IIPCFAVGRMQEVMLILKDLPYDMWVDGMGKTVNGYYTDRPDYLRNIKEFKAAKRRYN
                     PVRTASAREKARQAQVIITTSGMLDGGPVMHYLMDQKDNAKSAVMMVGYQAENSNGRM
                     LRERGMVDTPDGLMKVSCEVMTFDLSAHADHDQLLDFIKGCHPEKVVLMHSDNREELA
                     QDLEGDYEVLMPMSGDTFEL"
     gene            8396..9211
                     /gene="nadC"
                     /locus_tag="PLJ11_01675"
     CDS             8396..9211
                     /gene="nadC"
                     /locus_tag="PLJ11_01675"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOO03414.1"
                     /translation="MDLIDLYLQEDLREGDITSLALVDDRDGKAMITAGQDGVLAGVE
                     EAVEVFRRVGCRATPLIRDGDRVSEGQRVMEVQGPLKGLLAGERLALNFLMRMSGIAT
                     LTRQVVDSCRKANPNIVIAATRKTTPGFRRYEKKAVRIGGGDPHRFGLDDAFLIKDNH
                     LTVVGSVFKAVRKAQSYSFGKKVEIEVEDLEAAKEAARASADIILLDNMTPAQAKECY
                     LAIKAIDQRITVEVSGGIAPKSADGYARSADVISLGALTHSAPALPLSMHILE"
     gene            9431..9610
                     /locus_tag="PLJ11_01680"
     CDS             9431..9610
                     /locus_tag="PLJ11_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03415.1"
                     /translation="MDFEKHQCQFCGRPAKQYVFAAFCCESEDCVDKAREERGGPGGH
                     MKAKIHGGFVDIKER"
     gene            9662..10366
                     /locus_tag="PLJ11_01685"
     CDS             9662..10366
                     /locus_tag="PLJ11_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOO03416.1"
                     /translation="MRARIAGFAKTSLLDWDGMVAATVYLQGCNMRCPFCHNPDLVSV
                     STDAEEVPWEALEEYLRENSDFLDGVVVTGGEPTLNEDLPELVRRIKALGLKVKLDTN
                     GTNPGMLQDLLQAGLLDYVAMDLKGPLDQQYSQVCGTEVDLEAVKRSIQLIMGSGIDY
                     EFRTTVVPHYLNPGDVERMAAYIGGARKYALQRFKRERTLDKRLSQIDPYSEGQMLGM
                     AEVARKYVRKVVVRGI"
     gene            10432..11148
                     /locus_tag="PLJ11_01690"
     CDS             10432..11148
                     /locus_tag="PLJ11_01690"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOO03417.1"
                     /translation="MEAGDGRLKTYIDGFDRILEGGIPKGHIVLVAGTPGTMKSSVSY
                     YMLYNHAVESGITSVYVTLEQGRESLLRQMEKMGMDVERVKDNLHVLDLAIIRKRLKE
                     VAGGTSWLHVFKTYIANLKQNLDFSLLVIDSMEVLETMAEIRNRRTDLFYLFEWLRDL
                     GVTVLLISESSSERLIEGKFDEGYLSDGIITLKMQLIRDVEMQRRIRCVKMRETNHDP
                     SYYSLLYNNGRFQVTRVISE"
     gene            11153..12454
                     /locus_tag="PLJ11_01695"
     CDS             11153..12454
                     /locus_tag="PLJ11_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOO03418.1"
                     /translation="MVRYECPRCGFGVRPGDENCGRCGEDLKPKSVQPIQVTLGAVQL
                     DRGQGERYNAHIRSVDSIQVARPDSTIQAKKAELDRREQALLERERKLRAFAKELEER
                     SKAVGPAVPPAAPDMDELRRRVREELARQFEPELEALQAQLDEREAELQEARLKLQMT
                     EACQTIEGVDEVELARITEEIFTELRAQLRSPPKVSDVGVLRTRIPRLDEVLGGGIPE
                     GHVVLLSGPPGTMKSTLAYTILYKAAVNGLSALYLSVEQGRRSIVRQMERMGMPEAGT
                     EGRLKVMDLRELRREMEGSEGNWREVIMGYVQREQEATGFKAFALDSLESFKALTEHC
                     FSRQDLKDLFDWFKALGITVLVISENVPDEWDEDYQGEAYLSDGILELLMREMGDSRV
                     QRWVRCVKMRGANVDNRYYTMFHDGREFSLSLPLAPQPCVR"
     gene            12537..13250
                     /locus_tag="PLJ11_01700"
     CDS             12537..13250
                     /locus_tag="PLJ11_01700"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HOO03419.1"
                     /translation="MAIERVRTYIHNFDEYIQGGVPRGHVILIAGTPGTMKSSVCYSI
                     LYQNAKHNGTRSVYMTLEQSKENLLQQMEFMGMNDPEVQEKVHILDLATIRKNVTEMM
                     AKGSWLQVFKMYAEAVRKDVGYDLLVVDSLDVLEMAAQMNSDRRNDLFYLFMWLRGMS
                     VTSFLISETSVDTINGGRFEEGYLADGVISLKLQSIGETDVQRRIRTVKLRSTNHKTG
                     YFSLMFSDGTFRATQVINE"
     gene            13307..13382
                     /locus_tag="PLJ11_01705"
     tRNA            13307..13382
                     /locus_tag="PLJ11_01705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13340..13342,aa:Val,seq:cac)
BASE COUNT         2590 a         4202 c         4232 g         2381 t
ORIGIN      
        1 atagccatcg gcggccagga gcgccctgca ttccatgtcc tccccatcga agcacgccct
       61 gataccgtcg cccgtccgct ccaccgcccc gcacctcacc cccgtccaga cctccgcgcc
      121 cgccctctcg gccaaggacg cgcagcgcct gtcgagcgcg ccgcggtcca ccaccaccgc
      181 ctttacctcg ttcccctgga gctctagggt ccgcccgccg gggaaatgaa cgtacgcccc
      241 gtcgatgcgg ttgaggacgg catcgggggc ggaggccatc tccaccaccc tcggggtgac
      301 gaggccggcg cactgcacag gctcccctat cctgggatgc tcctcgacca ccgtcacctc
      361 gagctcggcg gccatcgccg ccgcggccag gcagcccgtt ggtccccctc ctacaacaag
      421 gacgtcccgt cgcatcccgt tcctcctggc atccgggccc tcatgggccg cgcctcgaca
      481 tgggccagcc cggggtccag gcgcctactt ggggaccttc tcccagtcct tgaggaagcg
      541 ttcgatgccg atgtccgtca gggggtgctt gaacatcttc ttgagcacgt cataggggat
      601 ggtggctatg tgcgcgccca tgcgggccgc ctcagccaca tggatcggat ggcgtacgct
      661 cgccacgatc acctccgtgt cgaaatcata gttgtcaagg atatccatga tctcggagac
      721 gagggccatc ccgtcctggc cgacgtcgtc caggcgcccc acgaacgggg acacgtaccg
      781 ggcgccggcc ttggccgcca gcaatgcctg gttcgccgag aatatcaggg taacgttgac
      841 cgagaccccc tcgttcgaga gcatcttggt ggccttcagc ccctcctcgg tcatggggac
      901 cttgatcacg atgttgtcgt tcaccgcggc cagccgcatc ccctcggcga acatctcctc
      961 ggccctgagg ctgacgacct cgacgctgat gggaccgtcc acgacctcac agatctcgtt
     1021 cacgatggtg gcgaggtcct tcctctcctt ggctatcagg ctggggttcg tggacgccgt
     1081 tcaggatccc ccaggagctg acctccctta tctgctcgat gttggctgtg tcgatgaata
     1141 tcttcatttc ttcctctcca tggccttgat ggcggcggtg agtacctccc ggtcggtgag
     1201 gccgtactcc cgcatcagct cctcactctc ccccgactgc ccgaacacgt cctgcatggc
     1261 aacccgctcc atcgggaccg ggtacctctc cgcgagcgcg gcggccacaa gggtgcccac
     1321 gcccgaaacg atgttgtgct cctcggcggt gacgaccgcc ccgcacccgc gggcggcctt
     1381 ctccagcgcc tccacgtcta acggcttgat ggtgtgcagg tcgatgacct tggcgctgac
     1441 ccccctggcc ttcagggctt cggcggccag aaggcacgcc gcgaccatct ggcccgtgcc
     1501 caccagggcc acatcggccc cctccctgag ggtgtgcgcc cttcctatct cgaacctctc
     1561 ctccttcgag gtcaccaccg gaacgtcccc ccgtcccatg cgcacatagg ctggcccatg
     1621 ccgctccgcg accgccagca cggccttgta tgtctccggt ccgtcggcag ggacgacgac
     1681 cgtcatgttg ggcagggcgt tcatcagggc gatgtcctcc atggtctggt gggaggcgcc
     1741 gtcgcccccg acggtgatgc ccgcatgcga ggcgacgatc ttgacgttca ggtcggggta
     1801 ggcgatcgac tgcctcacct gatcataggc ccggccggtg gcgaacaccg caaaggtgga
     1861 ggcgaagacg gtcattccgc cgacggctag cccggcggcg gtgcccatca tgttctgctc
     1921 cgcgatgccg cagttgaaga agcgccccgg gaaggcgccc ttgaactcca cggtgcgggt
     1981 ggagctggag aggtcggcgt ccagcacgac gacgtccccc cgcgccctgc cgaggtcgac
     2041 cagcgccctt ccgtagctct tgcgctgcgc ttccatcggc cagctcatac ctctcccccc
     2101 agctcgcgca tggcctgctc gtactccttc gcattgggcg ccttgccatg gaacccgacg
     2161 ttgttctcca tgaacgacac gcccttgccc ttcacggtcc tggcgatgat aacggtcggg
     2221 gagcccttat gcccctttgc ctcggccagg gtgtccaaga cctgccacat atcgttcccg
     2281 tccatgaata ggacgtgcca tccgaacgcc cgccacttgt cgcccaacgg ctccagggac
     2341 atgacttcct cggtgcgccc gtcgatctgc agcccgttgc ggtcgacgat ggcgatcacg
     2401 ttgtcaagat gatagtgggc ggcgaacatg gccgcctccc agacctggcc ttcggccatc
     2461 tccccatccc cgcagaggca gtacaccttg cgatccagcc ccaggaggcg aagcgccagc
     2521 gccaggccgt tggcgacgct cagcccctgc ccgagggaac cggtcgacat ctcgaccccc
     2581 ggggtcttga ccctggaggg atgcccctgc aaccgggaat ggagcttacg cagtgtcagg
     2641 agctcctcgg tcggaaagta cccgcactcg gcgagggctg catagtacac gggggcggca
     2701 tgccccttgg acaggatgaa atggtccctg tcctgcgagc ccatccgctc gggcgagtgg
     2761 tccattacct tgaagaagag ggcggcgatc acgtccgcgc aggacagcga ccctcccgga
     2821 tgcccggacc cggcggcgta gatcatccta ataacatgcc gacgcaaggc aagggccttg
     2881 gcctcaagct cactgacgga tatctggtcg ggggaattca aagcggttca cctgaatggg
     2941 atgggaggac cctcattaac cggggggcac ataaagatat tttggtcctc ccagcgcaat
     3001 tgtcaggaat agggggcggg aaggggccca tgggcggggc gcatccctcg ggaggaaggg
     3061 ccgcgaactg attatatcct ccgcatccca tgccggtcca tggtcgccat cgaggtcccc
     3121 tgccttgcca acgaacggtt gaaggccgtc ctggagcgca tcaacgccga cgaggagctc
     3181 agggctctgt ggaaggcctc caacatcaac gccatcgacc gcctggggta tagcgaccac
     3241 ggctccacgc acgtgcgcat cgtcgcccgc aacgccctcc ggatgctccg gctgctgatg
     3301 gaggcggggc accggcccgg cgcggtgctg aactacggga tgcgtccgga ggatgccgag
     3361 gtggtcgtgg tgctggcatc ggcattgcac gatatcggcc tgtcggtcca ccgccagaac
     3421 cacgaggagt tctccgtcat gctgtccgcc cccatcctcc gcaggctcct cggaaacgta
     3481 taccaggagc ccgaactgac cttcgtggtc gccgaggtcc agcacgccat gatctcccat
     3541 catgagggct tcgcccccta cacgatcgag ggcggcgtgg tccgcgtcgc ggacgccctg
     3601 gacatggaga agggaagggc acggatcccc ttcgccagcg gctcggtgaa catccactcg
     3661 gtaagtgccc tggccatcga taaggtgcgg gtggggaagg gcaaggagcg ccccatcctc
     3721 atcgacatca cgatgaacaa ctcggccggg atctatcaag tggacgaact gctgaaggag
     3781 aagatagcgc gctcgggcct gagggaactc atcaccgtgg aggtgaaggt cccggaggcg
     3841 gagagcagga tagtgaagga gctgcgcctt tagtcgttct cgatcctggg cgccaacagg
     3901 taccgcacgc tgcccttgcc ccccgcgatg tcgaagtcga gccggaccgg caggtcattg
     3961 cccaggttga tggtgacgtt ggtcccgctg gggatggcct tgatgatggt cgagaaatag
     4021 tccatcggga agagggagcg gatgctctcc gagcactgaa ggtccaccag cagcgccttg
     4081 ggcagcttca ggctgaccga gtcggtgtcg ccgtcggaga ccatctcgaa cccttccgca
     4141 ttagcgttca gcgccacatg gtcggagatg ctctcggcgg ccttgatgcc cctctggagc
     4201 tcgtcgacgg cgatcgtgat gcgcgccgga agggtgatgt tggggacctt gggatcgctc
     4261 atgccctcgg tgctcaccag gttcatgcgg cgggtgacgt tgccgacagt gattaccagg
     4321 cggccgcgtt ccacatcatg gcgcatatcg atgatgtcgc cagacctgga caggcggatg
     4381 acctccttga gcttctctag atccagtccg aggtcggtct cggtcgccga gtactgctcg
     4441 aaggcctcct tgctcaggtt aaggtcgacc atggccacat gggctgggtc cacagccttg
     4501 agctggatgc cgttctcgcc tatcttgaac ttggcctcgt cgatgagcgt ggaaacgacg
     4561 tcaacgaccc ccttgagggt atcggatctg agtttggcct gaaacattgg gtccccttcc
     4621 tccacagtaa agccctgtgc taattataac ttttctgcga acgacaggcc tcaatactca
     4681 cgccaggaat ggccgcagga gacgcaccta tagatcttgg tctccggctc gtccgccgcc
     4741 ctggtctgcc gtaggaccca gaacgcctcc tgatagccgc acttcggaca gtctatccgg
     4801 gtgcgcggca gggtgggggc gttcccatcg ataacggcta tctccttctc cttcccgctc
     4861 gtggtgaagc agaccggttc cccgttcttg atgacctcat gcttgcagtc cgggttcctg
     4921 cacacaaact tcccattgtc cggcttcatt atcttatggc acttagggca gaacatcctt
     4981 ctctcgccaa aggaccacaa atgatagggc tataaaaaat tatccagctg acctagatga
     5041 acttccaatc gcctggcttc aggttcttgc cctttccgcc gcccttggac cgcttcgcca
     5101 gcccggccat gttccggggg atctcctctt caggctcctc gccctcttcg taccatccct
     5161 cccccttctc ggagtactcc tcggcatacc cgtcgtcgct ggcggtggtc aggtcctcga
     5221 ggtccgagaa acggatgacc cgctgctcct gcctcgtcct gatgggcctg gccgagaccg
     5281 gacggctccc gaccgccggg cgggaagcct ccggctcccc cttccccctc tctccgttgc
     5341 tcacgaccac ggtcatggag ttgccgccgt gcttggccac atagcccatc tccaactggg
     5401 actgttcggc caagatgccg cctatgtagg cgaaggcggc ggtgatgatg tagacgtcga
     5461 cgtggacgcc cgcggagatc tggatcgcat cgatgaggga ggtcatgtac atctggacga
     5521 agacgatgta gggggcgagg accggtaccg ctctctcgac ccacgccaga agatgcgagg
     5581 gggtccagtc caccccattg atgaccgtgg ggatggtccc gctgtccacc agcagggtga
     5641 tcgtgaagat aaccgtaagg ggtacaaggg cggccaatac ccctttccat ggcttgcccg
     5701 ccctcctccc gctgacgaac ccggccacca tctgccctag gagagggagc caccaaagga
     5761 gcacggtcag gatgaaggag tacctcaccg cgctccagaa cgaatagggg accttgacgt
     5821 ccctatatct tttgatctcc tcgtcctcat agacctcgga caggcggtag accccgcggt
     5881 caatgagggt gttagttatc tcatcgtccg catcggcgct acgcctgaat cccatcccat
     5941 ccccttcagt cggacatatg tccgacaata gcaatatagc tcggttacta taaatccttt
     6001 tgtcaggatg aatggggggc cagcatcaac gggggatgta cccgctgagc ccatacatgg
     6061 ccagggcgct cttggtgctc tctggggtca cgcggttggg ctgggtggca tacctcatga
     6121 tctcctcgat gggatattcc agaaagtcag ccaggagctt gaggcgcgcg agggggaagg
     6181 cctgcttccc ctgcatgatc tggacgacgc cccacccagg atgcacgcgg gagcgatagc
     6241 ccagcctcct ccccagttca ttgacggaac cggccttctc catccccatc ttgatgagcc
     6301 ggacccggaa ggcggagctt atccacacac ggtcggtgca aaggggggcg cccccgccgt
     6361 agcccattgc gaactcggcc atggtcattc atggcctatc acaatataaa atcgtttact
     6421 tcgacccctt ccaaggtcca agccccggct caggggaggc ggccaccaaa aatggtttat
     6481 acggattagg agtagcctga ctgagcaaat gggaacgttc cgcacctcca ctgtcatcga
     6541 gacccaaggc gagggggacc tggtggacgt gannnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnttccgcac ctccactgtc atcgagaccc aaggcgaggg ggacctggtg
     6721 gacgtgacat ccttggtcga aaggactgtg actggctccg gcctccgaca ggggatcgcc
     6781 ttggtgttca ctccgcattc caccgcagcg gtcgtgaccc tggagaacga gcccggcctg
     6841 cgagaggacc tcttccaggc ccttgaaagg aactttccca aaggctcgga gtaccagcat
     6901 cacctgcgat ggggggacgg gaacgggcac tcccacatcc gctcgacctt cctgggggcg
     6961 tcggtcgccc tgcccttcga cggggggcgg ctcgagcggg gggaatggca gcaggtggcc
     7021 ctactggagc tggacgtcag agggcggagg cgagaggtca tcgtgcagct cctgggcgaa
     7081 tgaccatcat cacacatcac gatctacgag gtaaacgaaa tgaaagtgaa gttcttaggg
     7141 ggcgcggaga tagtcggcaa gctggggatg cttgtcgaga acaacggcgc cagcatcatg
     7201 ttcgagtacg ggctgcggct gatcaagaag gaacctccgc agttcccgat ggaatccccg
     7261 cgcgtggagg gcgtgttcgt cacccactct catctggacc atagcggggc ggtgcccagg
     7321 ctggtccagg acaccgactg cgacgtctac tgtaacgatc tgacaatgga cgtggcgtcc
     7381 ctgctgttct tcgactccat caagatcgcc aaatcggagg ggtacgagaa catattgcgg
     7441 tacaacgatg gggacgtaaa gcacgtctgg cagaactacc gttccttcga cgtcggcgac
     7501 gtgaagccca tcgccgggct cgagatcgtc ccccacaacg ccggacacat ccccggggcc
     7561 cagatgtacg aggtaaaggg gcatgacacc accctcttca ccggggacct gcacaccgcg
     7621 aacacccggc tcacgtatgg cgcgaagccg gtcgagtgcg acaacctggt catagaggcc
     7681 acatacgctg ggaggaacca tcccccccgg gagaagagcg aggcccttct ccagcagaag
     7741 gtgagggagg tcgtggagcg cggagggacg gccatcatcc cctgcttcgc ggtgggcagg
     7801 atgcaggagg tcatgctcat cctgaaggac ctcccgtacg acatgtgggt ggatggtatg
     7861 ggcaagaccg tgaacggcta ctacaccgac cgtcctgact atctgaggaa tatcaaggag
     7921 ttcaaggcgg ccaagcgcag gtacaaccca gtgcggaccg cctctgcccg ggagaaggcc
     7981 cgccaggccc aggtcatcat cacgacgtcc gggatgctgg acggaggccc ggtgatgcac
     8041 tatctgatgg accagaagga caacgccaag agcgcggtca tgatggtcgg gtaccaggcg
     8101 gagaactcca acgggcgcat gctgagggag aggggcatgg tggacacccc ggacggcctc
     8161 atgaaggtga gctgcgaggt catgaccttc gacctctccg cccacgctga ccacgatcag
     8221 ttgctcgact tcatcaaggg atgccatccg gagaaggtgg tcctcatgca ctccgacaac
     8281 cgggaggagc tggcgcagga cctggaaggg gactacgagg tgctcatgcc gatgagcggg
     8341 gacaccttcg aactctgatg ccatccttga tatggaccca ggggaatgaa ggggcatgga
     8401 cctcatcgac ctttatctcc aggaggacct gcgggagggg gacataacct ccctcgccct
     8461 ggtggacgac cgggacggga aggccatgat caccgccggc caggacggcg tgctggccgg
     8521 ggtcgaggag gccgtggagg tctttcgacg ggtgggatgc cgcgccaccc cgctgatccg
     8581 cgacggggac agggtctccg agggacagcg ggtgatggag gtccaggggc cgctcaaggg
     8641 attgctggcc ggggagcgcc tcgccctgaa cttcctgatg cgcatgagcg ggatagccac
     8701 cctcacccgc caggtcgtgg attcctgccg caaggccaat ccgaacatcg tgatcgcggc
     8761 aacccgcaag accacccccg ggttccggcg ctacgagaag aaggccgtca ggatcggagg
     8821 gggggacccg caccgcttcg ggctggacga cgccttcctg atcaaggata accacctgac
     8881 ggtcgtcggc agcgtcttca aggcggtgcg caaagcccag tcctattcct tcggaaagaa
     8941 ggtggagatc gaggtggagg acctggaagc ggccaaggag gccgccaggg cgtcggccga
     9001 catcatcctg ctggacaaca tgacccccgc ccaggccaag gagtgctatt tggccataaa
     9061 ggccatcgac cagcgcataa cggtggaggt ctccggcggg atagcaccga agagcgccga
     9121 tggctacgcg cgttccgccg acgtgatatc cctgggcgcg ctgacgcatt ctgccccggc
     9181 attgcccctc tccatgcaca tcctggaatg atctgtaccg cgcggtgcgc ccgcgccctc
     9241 ggcaatgaag aggaaagctt ctgctgaata tgcccgatga aggcggagat gcctatgaat
     9301 ccagggctac gaacccggcg tcattgacat gctctccgta cattgaatgg caccttattc
     9361 cccccaggca aacggaccct ccatcaggaa catcgaaagg gttttatcgc tccgtttcgg
     9421 tggacacacc atggatttcg agaagcatca atgccagttc tgtggccgac cggccaagca
     9481 gtacgtgttc gcggccttct gctgcgagag cgaggactgc gtggacaagg cccgcgaaga
     9541 gaggggcgga cccggcgggc atatgaaggc caagatccac gggggcttcg tggacatcaa
     9601 ggaaaggtga gcgcccctcg gggataccct taaaatcgac ctcaccgtat tccgaagggc
     9661 tatgcgcgcc aggatcgccg gcttcgccaa gacctcgctc ctcgactggg acggaatggt
     9721 cgccgccacc gtctacctgc agggctgcaa catgcgctgt cccttctgcc ataatccgga
     9781 cctggtgtcc gtctcaaccg atgcggagga ggtgccctgg gaggcgctgg aggagtacct
     9841 gcgggagaac tccgatttcc tggacggggt ggtggtcacc ggcggggagc cgaccctgaa
     9901 cgaggacctc cccgagctgg tccgaaggat caaggccttg ggcctgaagg taaagctgga
     9961 caccaacggc accaaccccg gcatgctgca ggacctgctc caggccgggc tgctggacta
    10021 cgtggccatg gacctcaagg gcccgctgga ccagcagtac tcccaggtat gcgggacgga
    10081 ggtggacctg gaggcggtga agcgctccat ccagctcatc atgggctccg ggatcgacta
    10141 cgagttccgg accacggtcg tgccgcacta cctcaacccc ggggacgtag agcgcatggc
    10201 cgcctacatc ggcggggcga ggaagtacgc cctccagagg ttcaagaggg agcgaacgct
    10261 ggacaaacgg ctcagccaga tcgaccccta ttccgaaggg cagatgctgg ggatggcgga
    10321 ggtcgcccgg aagtacgtgc gcaaggtggt cgtccgcggg atatgagggg gccagcgagc
    10381 ctttattaga cggtagggca atgtgcccct aataggcctg agggagagcg gatggaagcg
    10441 ggcgacggcc gtttgaagac ctacatcgat gggttcgaca ggatcctgga gggagggatc
    10501 cctaaggggc acatagttct ggtggcgggg acgccgggga cgatgaaatc gtccgtaagc
    10561 tattacatgc tctacaatca cgccgtggag tcggggatca cctcggtgta cgtgaccctg
    10621 gagcagggca gggagagcct gctgcgccag atggagaaaa tgggcatgga cgtcgagagg
    10681 gtcaaggaca acctgcacgt gctggacctg gccatcatcc gcaagaggct gaaggaggtg
    10741 gcggggggga cctcctggct gcacgtcttc aagacctata tcgctaacct caagcagaac
    10801 ctggacttca gcctcctcgt cattgattcc atggaggtcc tggagaccat ggccgagatc
    10861 cggaacaggc gcaccgacct cttctacctg ttcgaatggc tgcgggacct cggcgtcacg
    10921 gtgctcctca tctccgagag ctcgtccgag cgcctcatcg agggtaagtt cgacgagggg
    10981 tacctgtccg acgggatcat cacgctcaag atgcagctga tccgggacgt agagatgcag
    11041 aggcggatcc gctgcgtgaa gatgagggag accaaccacg acccctcgta ctactccctg
    11101 ctctacaaca acggccgctt ccaggtcacc agggtaataa gcgagtgatc cgatggtacg
    11161 gtacgaatgc ccacggtgcg ggtttggggt ccggccgggg gacgagaact gcggccggtg
    11221 cggcgaggac ctgaagccga agtccgtcca gcccatccag gtcacccttg gggctgtcca
    11281 gctggaccgg ggccaaggcg agcgctacaa cgcgcacatc cgctccgtag actccatcca
    11341 ggtggccagg ccggacagca ccatccaggc caagaaggcc gagctggaca ggcgcgagca
    11401 ggcgctcttg gagagggagc ggaagctcag ggcgttcgcc aaggaactgg aggagcgctc
    11461 caaggccgtc gggccggcgg tgccaccggc cgcgccggac atggacgagc tccggcgcag
    11521 agtgcgcgag gagctggctc ggcagttcga gccggagctg gaagcgctgc aggcccagct
    11581 ggacgagcgc gaggcggagc tgcaggaggc ccggctcaag ctccagatga ccgaggcgtg
    11641 ccaaaccatc gagggggtgg acgaggtcga gctggcgcgg atcactgagg agatattcac
    11701 tgagctgcgg gcgcagctca ggagcccgcc aaaggtcagt gatgtgggcg tgctccgaac
    11761 gcgcatcccg aggctggacg aggtcctggg cggagggata ccggaggggc acgtcgtcct
    11821 tctgagcggc ccccccggca cgatgaagag caccctggcc tacaccatcc tgtacaaggc
    11881 cgcggtgaac ggcctttccg ccctgtacct ctcggtggag cagggtcggc gctccatcgt
    11941 ccggcagatg gagcgcatgg gcatgcccga ggcgggcacg gaggggaggc tgaaggtcat
    12001 ggacctccgc gaactccgca gggagatgga ggggagcgaa gggaactgga gggaggtcat
    12061 aatggggtac gtgcaaaggg agcaggaggc cacaggcttc aaggccttcg ccctcgactc
    12121 cctggagtcc ttcaaggccc tgacggaaca ttgcttctcc aggcaggacc tcaaggacct
    12181 cttcgactgg ttcaaggccc tcggcatcac cgtcctcgtc atctcggaga acgtccccga
    12241 cgaatgggac gaggactacc agggggaggc gtacctctcg gacggcatcc tggagctgct
    12301 gatgcgggag atgggcgact cccgcgtgca gcgctgggtg cgctgcgtga agatgcgggg
    12361 ggccaacgtg gacaaccgct actacaccat gttccatgac gggagggagt tctccctgtc
    12421 gctgccgctg gccccccagc cttgcgtccg atgatcgggg gccgaaaagg tcatttgcga
    12481 tggccttgaa aacggtaata taccctggaa cgtaatggtt cgccagagga cgggccatgg
    12541 cgatagagag ggtgaggacc tacatccaca acttcgacga gtacatccag ggaggggttc
    12601 ccagggggca tgtaatcctc atcgcgggca cccccgggac catgaagtcg tcggtctgct
    12661 acagcatcct ctaccagaac gccaagcaca atggcacgcg gagcgtgtac atgacgctgg
    12721 agcagagcaa ggagaacctg ctgcagcaga tggagttcat gggcatgaac gacccggagg
    12781 tgcaggagaa ggtgcacatc ctcgacctcg cgaccatccg gaagaacgtc acggagatga
    12841 tggcgaaggg ctcctggctc caggtcttca agatgtacgc cgaggcggtg aggaaggacg
    12901 tgggctacga cctcctggtc gtggactccc tggacgtact ggagatggcc gcgcagatga
    12961 acagcgaccg gcgcaacgac ctcttctacc tcttcatgtg gctccggggg atgagcgtca
    13021 cgtccttcct gatctcggag acctccgtgg acacgatcaa cggcgggcgg ttcgaggagg
    13081 gctacctggc cgatggcgtg atctccctga agctgcagag catcggggag acggacgtgc
    13141 agcgccgcat ccggacggtg aagctgcgct ccaccaacca caagaccggc tacttctccc
    13201 tgatgttcag cgacgggacg ttccgggcca cccaggtcat caacgaatga ggagggggca
    13261 tcgctaaaca tttttatatc gcgttcccgt tcgctccgaa tgcctagggc tcatggtcta
    13321 gcggttatga cgtctgcttc acacgcagga ggtcgccggt tcgaatccgg ctgagcccac
    13381 cacatctctt atccagacaa ttattttttg aatttactca tgttgcatgc tttttaaact
    13441 ggctatcgaa cgataccgat cgattgaaat ttacttttta atcatctgga tcaatcgatg
    13501 cgtgg
//
LOCUS       DAOSYN010000019        12694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_4166_length_12694_cov_4.201282, whole genome shotgun sequence.
ACCESSION   DAOSYN010000019 DAOSYN010000000
VERSION     DAOSYN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12694
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4166_length_12694_cov_4.201282"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..597)
                     /locus_tag="PLJ11_01710"
     CDS             complement(46..597)
                     /locus_tag="PLJ11_01710"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HOO03420.1"
                     /translation="MSEELVGSPFPDCVLRNERGEEVRLSEQWAEGSVLLLFFSSAFG
                     FVCNLEMLTFTAMLKAFQAANCRIFGVSNDSVRSMGAYSESLRIGFPLLSDEGARLAR
                     RLGAMEPDDGPWPGFPMRSEFLIDRRGIIRYAHIPPDSNWEPDYDEILALARSLYEED
                     RKAKGEGPGGDAPEASEGRPSPP"
     gene            complement(732..809)
                     /locus_tag="PLJ11_01715"
     tRNA            complement(732..809)
                     /locus_tag="PLJ11_01715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(771..773),aa:Pro,seq:tgg)
     gene            complement(1048..2016)
                     /locus_tag="PLJ11_01720"
     CDS             complement(1048..2016)
                     /locus_tag="PLJ11_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOO03421.1"
                     /translation="MRVRGSTIVVTGCAGFIGSHLTDALLALGNRVTGVDDLSAGRRE
                     FLENAEGYPEFRFVRADLLSDDLRPVLKGADAVMHLAANPDVRSGASDTLAHYRQNIE
                     ATYRVLEAMRATGVKGILFPSTSTVYGEPAVIPTPEDYGPLVPISVYGATKLACEALV
                     CSYAHSFDMEAVIFRFANVVGARSTHNVLHDFVAKLRADPSRLEILGAAPGTTKSYVH
                     VEDCVRGMVIGAEYARSRVEIFNLGSNDRLSVKEIADMVTEEMGLRGVRYEWTGGVQG
                     GRGWIGDVKDMLLSSDKLRSYGWEPAMNSSQAIRKAVREVIGGGAV"
     gene            complement(2013..2579)
                     /gene="rdgB"
                     /locus_tag="PLJ11_01725"
     CDS             complement(2013..2579)
                     /gene="rdgB"
                     /locus_tag="PLJ11_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOO03422.1"
                     /translation="MKLGLVTSNRHKLEEYRHGLSLLGIEVEHLDADCDEIQADTLQE
                     VVLACIRQLREAGHRNFMLDDSGLFVPSLNGFPGVYSAYVMQTIGCQGMLRLLEGLDR
                     RARFECCIGVCSDELGDFTVTGMAPGRIVHEERGSGGFGYDPIFAPEGQELTFAEMGM
                     AEKNERSHRGKAMALLAAELRRRMGGGA"
     gene            complement(2576..3202)
                     /locus_tag="PLJ11_01730"
     CDS             complement(2576..3202)
                     /locus_tag="PLJ11_01730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF011462.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HOO03423.1"
                     /translation="MNASVNVLRRGAEAEIRRDTFLGRSVLVKSRVPKPYRHPSLDIS
                     LRSHRTRTEARLLQEARECGVPTPIVYDIDLAKAELWMQEVPGPRAKDALEAAGPEEV
                     RAICQEIGRMVARLHSHGMTHGDLTTSNMILHEGRVWFIDLSLGAKAASLEEMGVDMH
                     LLREAFQSAHSDRMYGYDVLAETYARELPIGAAVLAKAKEIEERGRYT"
     gene            complement(3215..4912)
                     /locus_tag="PLJ11_01735"
     CDS             complement(3215..4912)
                     /locus_tag="PLJ11_01735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOO03424.1"
                     /translation="MERVEDLFPFPAARRGQAELLRDARECVGAGKVLVAHAPTGLGK
                     TAVALAACLERSRRDGGRVVFLTPRQSQHRAVIETVRMMPAAIATVDLLSRESMCPAG
                     PRPPCLEGRACAFQTERRMTECAKEVLRRPLHAQELIALCLRRGACPYLTARLALTGA
                     DLVVGDLSRVFGDMPDVLRFQGASAPQYLVVDEAHNLPSRIMDMFSRELCIGPDGGGQ
                     LGEVWREMLSHGHRLIPTAELRSLLERHSLPEPEELEDEDPMVRDWLRLGEAAVRIAY
                     PEEGRIALRFLEPDLVVGSVLERCAAAIFMSGTLHPPELFAARMGIREGVCRSYPSPF
                     DPARRLVVAVPDVGTRFRDRCRRTTDEMALRICELADVVPGNVMVFLPSYSYMGAVRG
                     ALRRIGQRRTLLAERPGMSKAERDGLAGRLSCGREVLMLCNVHGSFSEGVDLPPGCLS
                     AVMVAGLPLPPPSLERREMVARASRKLEGGYDIDTYEALSRVVQACGRAIRREEDRAA
                     IVLLDPRYLDRKVVRMLPPDLTFADGDPVGLLRAFFEDEGRPLWKFHLEGERIITGH"
     gene            5031..5708
                     /locus_tag="PLJ11_01740"
     CDS             5031..5708
                     /locus_tag="PLJ11_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOO03425.1"
                     /translation="MKYTISGDNLQFVNIEFMPGEMIYSEAGSMVYMSGNVRMEAKAT
                     GGVLSGLKRAMAGESFFVTNFYADGGPGLVGFAGNVPGKVMAIDLRGGKQWVLQKTAF
                     LAAEASVALDIAFQRKFGAALFGGEGLILQKVYGEGTVFISGAGDFIEYNLLPGQSLK
                     VSTANVAAWEASVSYDIQSLGVKTALFGGEGLFVTTLTGPGKVVVQSMTISKLANSIL
                     PYLPNKG"
     gene            5713..5979
                     /locus_tag="PLJ11_01745"
     CDS             5713..5979
                     /locus_tag="PLJ11_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03426.1"
                     /translation="MAKASDVTIVALLAIGAMAVAILAIMHLDLLLAAIVVLVLVGLL
                     ALAVLLVLGGLAVVPYYFAKRGQDTHPGSYSLEQLKDNRDQGRK"
     gene            5981..6685
                     /gene="radB"
                     /locus_tag="PLJ11_01750"
     CDS             5981..6685
                     /gene="radB"
                     /locus_tag="PLJ11_01750"
                     /inference="COORDINATES: protein motif:HMM:NF020014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HOO03427.1"
                     /translation="MEEPRPVPFQCAPLDEMLDGGIESGCLTLIYGEAGTGKTNLCLA
                     LANNLAQQGRKTVYVDSEGVSLQRLRQMAGEDQERALKGVLVSEVHSLEEQDRMIGKA
                     IKLAEGNPDIGLIVVDSITMFYRLASKDDERAERRVLAGQSAKLLTLARKMNLPVVVT
                     SQVYTDVETGAFEALGGNVLHHNAKTIIRLEWLAPSKRRAVLMKHRHLAEGRAAVFYL
                     TEKGVEAEPQSSRPTA"
     gene            complement(6661..7647)
                     /locus_tag="PLJ11_01755"
     CDS             complement(6661..7647)
                     /locus_tag="PLJ11_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOO03428.1"
                     /translation="MDHSARTKEMIGDVDRALAGYLERGGHEKLIRAMRHYPEAGGKR
                     MRPTMAILVAEAVGGRGKEALPFGCALELIHNFTLVHDDVIDKDPVRRGRPAVHIAFD
                     EPTAIIAGDALFAIAFDVLATVEVDGERLRRLLRSVSNTVFLVAEGQQMDVDNEDRPT
                     VAREEYLDMVEKKTAVLFACAAEGGAIIGGGTEEQVRAMREYARLLGIGFQIWDDVLG
                     LKGDAKRVGKPVGSDIRNGKRTLIVVDALERLEGDGRKHILTDALGNADATDEQVRAA
                     VRLLEDIGSIERARQFALDYARRAKELLSCLRPSEEREMLREMVDYAVGREL"
     gene            complement(7653..8711)
                     /gene="fni"
                     /locus_tag="PLJ11_01760"
     CDS             complement(7653..8711)
                     /gene="fni"
                     /locus_tag="PLJ11_01760"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOO03429.1"
                     /translation="MKQIEQRKSDHIEVTLKEKVVPDYDYWSDVRLVHRALPEVDMDA
                     IDTSVTLFGKRLEMPLVVTAITGGFPLAKQINANLAEACAQAGVGLGVGSQRPAIENG
                     DDGTYSVVKDFDVPLVIGNVGAPQLVRQRNHRAFTLEEVLKAQEIVGADIMAIHLNFL
                     QEVVQPGGDVNASGCLEAIRALAGQGPCMVKETGAGISRETALMLKGTGIRGIDVSGV
                     GGTSFAAVEMHRAERKDDRVAMALGRTFFDWGIPAPAAIIEADVGLELIASGGIDDGL
                     RIAKGLALGADAAGCARAVLAEAMESSAAVLGKIERMKAELKAAMFLTGCSSVRELRE
                     RRVIVSGRTADWLTASEE"
     gene            complement(8708..9478)
                     /locus_tag="PLJ11_01765"
     CDS             complement(8708..9478)
                     /locus_tag="PLJ11_01765"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HOO03430.1"
                     /translation="MILVKLGGSVITDKGQYRRFDRETAGRLASEIAAAGERIVLVHG
                     AGSFGHILAKEHRLHLGITEDAQLRGAAEVMADVRELDLEMCRCLVHAGLPVVPLPPA
                     ACAVMRDGALERLELTTFLRYLDLGMVPMSFGDVVWDEVRGLSICSGDQLMMALAKEL
                     RPSRAIFVTDVDGVLTADPASDPGARLIERVDRGTLAALPRTQRNVDVTGSIYAKIQY
                     MIDMAEVAGECLVLNGKVPGRLEAALRGRKVVSSRVVS"
     gene            complement(9510..10319)
                     /locus_tag="PLJ11_01770"
     CDS             complement(9510..10319)
                     /locus_tag="PLJ11_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO03431.1"
                     /translation="MVLLDANEVLLIVLFVLLAVVIYFELRFMRSRNKDHIQRAMEKD
                     EVYNSIANAKAIASALRGRGRDTKEAEQTINLAEGAYHRNNFTSAKGLAMKAREMLMN
                     APLIEMAPPAPEAPAPAEERRTVHEIKKLEPNMMESRFLIRACRDRMEERSAAGHNVS
                     EAEEHLRNAERCFEEKRYDEALREGVRTRKLLDGEPAEAERPKDITLERLPPEKRCRK
                     CSTLIQPGDMFCRKCGEPATGRNCEGCSAELDEGDLFCPKCGRRVRTPLKD"
     gene            complement(10456..10576)
                     /gene="rrf"
                     /locus_tag="PLJ11_01775"
     rRNA            complement(10456..10576)
                     /gene="rrf"
                     /locus_tag="PLJ11_01775"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            11138..>12694
                     /locus_tag="PLJ11_01780"
     CDS             11138..>12694
                     /locus_tag="PLJ11_01780"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOO03432.1"
                     /translation="MLSIGVNTGTEVSYTSEHFLGYDLESGTWVNADLGKAYMEGDFV
                     SYQLRLDSDSKIWGAEEFSIKYNFYQAANDAIFVDGFDTSLATGFQYSTGDILMDGVQ
                     VPSAGWGTHIATPEAGESAGVTDPQIINYMDVWEVGDPDSEADPNDMRYFTVKNLPWE
                     DFTDGYVILFFRAHLALSILWRADLEQALPQVLDGDEFEDWTGISPHNGSSYTSGSSR
                     HFTLEYPGIGARTIPIPITSYPTDIITGRKFINEVPYDGWEMNMIGRLSLWGTTGADY
                     VYIPYNPPTVLTGTGPWALGYFAFDGLPRGNYTVWESVVDGYILDVIGTSPDLHLYDP
                     GKLPYFDAVTETWLDNPDEDLMVHFDLERGTKISPKVQNIDFYNLQVGDISGYKIHDT
                     DGDGVPDEGEPGLAGWTIELWEEDGLSAMDTTTTDNDGYFEFTDILVGQYIVKEVMQD
                     GWFNTTPVEISVCVSCGSDIDSLLFLNTMYSSICGYKVEDMNGNGVRDDSDPVIYDWE
                     IQLYKDDVLIN"
BASE COUNT         2324 a         4299 c         3760 g         2311 t
ORIGIN      
        1 ccccaacggt ccaagaatag atggcggccc gtccctgcca tccgcctatg gcggtgacgg
       61 tctcccctcg gacgcctccg gggcatcgcc ccccggtccc tcgcccttcg cctttctgtc
      121 ctcctcatag aggctgcggg ccagcgccaa tatctcatca tagtcgggct cccagttgga
      181 gtccggaggg atgtgcgcat aacggatgat gccccggcgg tcgatgagga actcagagcg
      241 catggggaag ccaggccacg ggccgtcgtc cggctccatc gcccccagcc tcctcgccaa
      301 cctggcgccc tcgtcgctca gcagggggaa ccctatcctc aggctctcgc tgtacgcccc
      361 catggagcgc acggaatcgt tgctcacccc gaagatgcgg cagttggcgg cctggaaggc
      421 cttgagcatg gcggtgaagg tgagcatctc cagattgcag acgaacccga aggcggagga
      481 gaagaagagg aggagcacgc tgccctccgc ccattgctcg ctgagccgga cctcctcccc
      541 ccgctcgttc ctcagaacgc agtccggaaa ggggcttccc accagttcct cgctcacacg
      601 accgctaggt cccagaggga taataatagg ttttggcgga agccacgccc cgggcctgcg
      661 gcggccgggg acgccccctc caagcctgcc ccgttgcccc taagggggaa cagctcctta
      721 aggaaaagag gtggtgaggc cgtccgaatt tgaatcggag tctcaggctc ccaaagcccg
      781 aaggatggac caagctaccc cacggcccct cggcggcgaa tcatccgtca gggataaata
      841 gttgcctggg ctggcatggg gggcccgcag ggccggtcct tcccttgcca tcgctcccag
      901 ccaaaccttc catggtacct tcgatccggt ccgacagggg gctggaccgc atcgcctgca
      961 ttggaaaagg cgctcgtccc acggcgcttc gcccgggggc ctcaagcatc aaggcggata
     1021 atgaacgcat ccgctcgttc gaaggcctta gaccgcgccc ccgccaatga cctcccgcac
     1081 cgccttcctg atggcctggg agctgttcat ggccggctcc cagccatagg agcggagctt
     1141 gtccgaggag agcagcatgt ccttcacatc cccgatccat ccgcgaccgc cctgcacccc
     1201 gccggtccat tcgtaccgga ccccgcggag ccccatctcc tccgtgacca tgtccgcgat
     1261 ctccttcacc gacaggcggt cgttcgatcc caggttgaag atctccaccc tgctccgggc
     1321 gtactcggcc ccgatgacca tcccacggac gcagtcctcc acgtgcacgt acgacttggt
     1381 cgtcccgggc gccgcgccca ggatctccag ccggctcggg tcggcccgca gcttggccac
     1441 gaagtcgtgc agcacgttgt gcgtgctgcg ggcgcccacc acgttggcga agcggaagat
     1501 caccgcctcc atgtcgaagg agtgagcgta ggagcagacc aacgcctcgc acgcgagctt
     1561 ggtggcgcca tagaccgaga tggggaccaa cgggccgtag tcctcgggcg tggggatcac
     1621 ggccggctcc ccgtacaccg tggacgtgga ggggaagaga atgcccttga ccccggtggc
     1681 cctcatcgcc tccaggaccc ggtaggtcgc ctcgatgttc tgcctgtaat gggccaaggt
     1741 gtccgatgcc cccgaccgaa cgtcgggatt ggccgcaagg tgcatcaccg cgtccgcccc
     1801 tttgaggacg gggcgcaggt cgtcgctcag caggtccgcc cggacgaagc ggaactcggg
     1861 atagccctcg gcgttctcca ggaactccct gcgcccggcg cttaggtcgt ccacccccgt
     1921 caccctgttc cccaacgcca gcagcgcgtc cgtaagatga ctgccgatga agcccgcgca
     1981 gccggtgacc acgatggtgc tccccctgac cctcatgccc ctccccccat cctcctcctc
     2041 agctccgcgg ccagcaatgc cattgccttc ccgcggtgcg agcgctcgtt cttctccgcc
     2101 atccccatct cggcgaacgt cagctcctgg ccctcggggg cgaagatggg gtcgtacccg
     2161 aagcccccgc tccctcgttc ctcatgcacg atccttccgg gcgccatccc ggtgaccgtg
     2221 aagtccccga gctcgtccga gcagacccct atgcagcact cgaagcgggc ccggcggtcc
     2281 agtccctcca ggagccggag catgccctgg cagccgatgg tctgcatgac gtaggcggaa
     2341 tagaccccgg ggaagccgtt cagcgaggga acgaacaggc cagaatcgtc cagcatgaag
     2401 ttgcgatgcc ctgcctcacg cagttgtcga atgcaggcca gcacgacctc ctgcaaagtg
     2461 tccgcctgga tctcgtcgca gtccgcatcc agatgctcca cttctattcc caggagggaa
     2521 agcccgtggc ggtactcctc cagcttgtgg cggttggacg tgaccagccc cagcttcatg
     2581 tgtaccgccc cctctcctcg atctccttgg ccttggccag cacggccgcc ccgatcggca
     2641 gctccctcgc ataggtctcg gcgaggacgt cgtagccgta catccggtcg gaatgggcgg
     2701 attggaaggc ctcgcgcaac aggtgcatgt ccacccccat ctcctccagg ctggctgcct
     2761 tggccccgag ggacaggtcg atgaaccata cccgcccctc gtgcaggatc atgttcgagg
     2821 tggtgaggtc cccatgggtc atcccatggg aatggagcct ggccaccatc ctgccgatct
     2881 cctggcagat cgcccggacc tcctcagggc ccgcggcctc cagagcgtcc ttggcccgcg
     2941 gcccggggac ctcctgcatc cagagctccg ccttggccag gtcgatgtcg tacacgatgg
     3001 gggtgggcac cccgcactcc ctggcctcct gcagcagcct ggcctcggtc ctggtccgat
     3061 gggagcgcag ggagatgtcc agtgatggat ggcgataggg cttcggcacc ctgctcttga
     3121 ccaggacgct ccgtcccagg aaggtgtccc ggcggatctc cgcctccgcc cccctcctta
     3181 gcacgttcac gctcgcgttc acgccgaagt aaagtcaatg ccccgttatt atcctttcac
     3241 cttccaggtg gaacttccaa aggggacggc cctcatcctc gaagaaggcc cgcagcagcc
     3301 ccaccgggtc cccgtccgcg aacgtgaggt ccggggggag catcctgacg accttcctat
     3361 ccaggtagcg ggggtccagg agcactatgg ccgcccggtc ctcctcccgg cggatggcgc
     3421 ggccgcacgc ctgcaccacc ctgctcaacg cctcgtacgt atcgatgtcg taccctcctt
     3481 ccagcttccg agaggcacgg gccaccatct ccctcctttc caggctggga gggggaaggg
     3541 gaagcccggc caccatcacc gccgagaggc agcctggagg aaggtccacg ccctcggaga
     3601 aggagccatg gacgttgcac agcatcagga cctcccgccc gcaggaaagc cggccggcca
     3661 gcccgtcccg ctccgccttg gacattcccg gccgctccgc cagcagcgtc ctgcgctgcc
     3721 cgatgcgccg gagcgccccg cgcaccgcgc ccatgtagga gtacgaggga aggaagacca
     3781 tcacgttgcc gggaacgacg tcggcaagct cgcagatgcg cagggccatc tcgtcggtcg
     3841 tccgccggca ccggtcccgg aacctggtcc ccacgtccgg caccgccacc accagcctcc
     3901 gcgcgggatc gaagggggac gggtaggaac ggcatacgcc ctcccggatg cccatccggg
     3961 cggcgaagag ctccggggga tgcaatgtgc cgctcatgaa gattgccgcg gcgcagcgct
     4021 cgagcacgct ccccaccacg aggtcgggct ccaggaagcg cagggcgatc cgtccctcct
     4081 ccggataggc gatgcgcacc gccgcctcgc ccagacggag ccagtcccgc accatggggt
     4141 cctcgtcctc aagctcctca ggctctggca ggctgtgcct ttccagaagg gaacgtagct
     4201 ccgccgtcgg gatcagccga tggccgtggg aaagcatctc cctccagacc tcgcccagct
     4261 gtcctcctcc gtccggcccg atgcacagct ccctgctgaa catgtccatt atcctggagg
     4321 gaaggttgtg cgcctcgtcc accaccaagt actgaggggc cgaggcgccc tggaaccgga
     4381 gcacgtccgg catatcgccg aacacccggg agaggtcccc gaccaccagg tccgcgccag
     4441 taagggccaa gcgcgccgtc aggtacgggc aggcgcccct cctgaggcaa agggcgatca
     4501 gctcctgggc gtgcaagggc ctccggagca cctccttggc gcactctgtc atcctccttt
     4561 cggtctggaa agcgcaggcc cgcccctcga ggcagggtgg cctcggccca gcgggacaca
     4621 tgctctccct ggagagcaga tcgaccgtag caatggccgc cggcatcatc cgcacggtct
     4681 cgatcaccgc ccggtgctgg gactgccggg gggtgaggaa caccaccctc ccgccatcgc
     4741 gcctggaccg ctccaggcag gcggccaggg ccacggccgt cttcccgagg ccggtcggcg
     4801 catgggccac caggaccttt ccggcaccca cgcattccct cgcatccctc aacagctcgg
     4861 cctgcccccg gcgggcggca gggaagggga agaggtcctc gacgcgctcc atgcacgccc
     4921 atccccccga tgtgggtagt tggttccctt tacgatgagc gtacgtcatc ccatagccca
     4981 ataaaaaaag tggaaatagc aacaaggccg tacccatccc cggtgatgtc ttgaagtata
     5041 ccataagcgg ggacaacctt cagttcgtga acatcgagtt catgcctgga gagatgatct
     5101 attccgaggc cgggagcatg gtctacatga gcggcaatgt gcgcatggag gccaaggcca
     5161 ccggcggcgt gctctccggt ctgaagaggg caatggccgg cgaatccttc ttcgtcacga
     5221 acttctacgc cgacggcggc cccggcctgg tgggcttcgc gggcaacgtc cccggaaagg
     5281 tcatggccat cgaccttagg ggcgggaagc agtgggtcct gcagaagacc gcgttcctgg
     5341 cggccgaggc gagcgtcgcc ctggacatcg ccttccagag gaagttcggc gccgccctgt
     5401 tcggagggga ggggcttatc cttcaaaagg tctacgggga gggcaccgtg ttcatctccg
     5461 gggcagggga cttcatcgag tacaacctcc tgccggggca gagcctcaag gtctccaccg
     5521 ccaacgtggc agcctgggag gcgtcggtct cctacgacat ccagagcctg ggcgtcaaga
     5581 cagccctgtt cggaggggag gggctcttcg tcacgacatt gaccggacct ggaaaggtcg
     5641 tggtgcagtc catgaccatc tccaagctgg ccaattccat actgccctat ctccccaaca
     5701 aggggtaggc gcatggcgaa ggcgtcggac gtcaccatag tggcgctgct cgccatcggg
     5761 gcgatggcgg tggccatatt ggccatcatg cacctcgacc tcttgctggc cgccatcgtg
     5821 gtgctcgtgc tcgtgggcct cctcgccctc gccgtgctcc ttgtcctggg cggtctggca
     5881 gtggtcccct actacttcgc gaagcgcggc caggacacgc acccgggctc ctactcgctg
     5941 gaacagctca aggacaacag ggaccagggg cggaagtgaa ttggaggagc cccgcccggt
     6001 cccgttccaa tgcgcccccc tggacgagat gctcgacggg gggatcgaga gcgggtgcct
     6061 caccctgatc tacggggagg cgggcaccgg caagaccaat ctgtgcctgg cattggccaa
     6121 caacctggcc caacagggca ggaaaaccgt ttacgtcgat tccgaggggg tctcgctgca
     6181 gcgcctcagg cagatggccg gggaggacca ggagcgggcg ctcaaggggg tcctcgtcag
     6241 cgaggtgcac agcctggagg agcaggaccg catgataggc aaggcgatca agctggcgga
     6301 ggggaacccg gacatagggc tgatagtcgt cgactccatc accatgttct atcgcctggc
     6361 gagcaaggac gacgagcgcg ctgagcgaag ggtgctggct ggccagagcg ccaagctcct
     6421 caccctggcc aggaagatga acctcccggt ggtggtcacc tctcaggtct acaccgacgt
     6481 ggagacgggg gccttcgagg cgctgggggg caacgtcctg caccacaacg ccaagaccat
     6541 cattcgattg gagtggctgg ccccgagcaa gcggagggcg gtcctgatga agcaccgcca
     6601 tctggcggag ggacgcgccg ccgtgttcta cctcacggag aagggtgtag aggccgagcc
     6661 tcagagctcg cgccccacgg cgtagtcgac catctcccgc agcatctccc tctcctccga
     6721 cggtctaagg caggacaata gctccttggc cctccgcgcg tagtccaggg cgaactgcct
     6781 ggcccgctcg atcgagccaa tgtcctccag aagccgcacc gcggcacgta cctgctcgtc
     6841 cgtggcgtcg gcgttgccca gggcgtcggt caggatatgc ttgcgcccat ccccctccaa
     6901 ccgctccagg gcgtccacga cgatgagggt gcgcttcccg ttccggatgt ccgagccgac
     6961 cggcttgccc accctcttgg catcgccctt gagccccagc acatcgtccc agatctggaa
     7021 gccgatgccc aggagccggg cgtactccct catggcccgg acctgctcct ccgtaccccc
     7081 gccgatgatc gccccgccct cggcagcgca ggcgaagagc accgccgtct tcttctccac
     7141 catgtccagg tattcctccc tggccaccgt cggccggtcc tcattgtcca cgtccatctg
     7201 ctgcccctcc gccacgagga agacggtgtt cgacacgctc cgcagcagcc ttcggaggcg
     7261 ctccccgtcc acctcgacgg tcgccagcac atcgaaggcg atggcgaaca gggcgtcccc
     7321 ggcgatgatc gccgtaggct cgtcgaaggc gatgtgcacc gccggcctcc cccgccggac
     7381 agggtccttg tcgatgacgt cgtcatgcac gagcgtgaag ttgtggatca gctcgagcgc
     7441 acatccgaac ggcagcgctt ccttccccct cccgcccacg gcctccgcga ccaggatggc
     7501 catggtcggc cgcatccgct tccccccggc ctcgggatag tggcgcatcg cccttatcag
     7561 cttctcatgc ccgccgcgct cgaggtatcc cgccagggcg cggtccacat cccctatcat
     7621 ctccttggtc cttgcgctgt ggtccattgg catcactcct ccgaggcggt cagccagtcg
     7681 gcggtccgcc cgcttacgat gacccggcgc tccctcagct ccctgacgct ggagcagccg
     7741 gtcaggaaca tggccgcctt gagctcggcc ttcatgcgtt cgatcttccc gaggacggcg
     7801 gcgctcgact ccatggcctc ggccaggacc gcccgggcgc acccggcggc gtccgccccc
     7861 agggccagcc ccttggctat gcgcaggccg tcatcgatcc cgccgctggc gatgagctcc
     7921 agcccgacat cggcctcgat gatggcggcg ggagcgggga tcccccagtc gaagaacgtc
     7981 ctccccaggg ccatcgccac ccggtcatcc ttccgctcgg cccggtgcat ctccaccgcg
     8041 gcgaagctgg tccccccgac gccggagaca tctatgcccc ttatccccgt ccccttgagc
     8101 atcagggcgg tttcccggga tatgccagca ccggtctcct taaccatgca cggtccctgc
     8161 ccggccaaag cccgaatcgc ctccaggcat ccgctggcgt tgacgtcccc cccgggctgc
     8221 acgacctcct gcaggaaatt gaggtggatg gccatgatgt ccgcccccac gatctcctgc
     8281 gccttgagga cctcttccag ggtgaaggcc cggtgattcc tctgcctgac gagctgaggc
     8341 gcgcccacgt tccctatcac caacgggacg tcgaagtcct tgaccaccga ataggtgccg
     8401 tcgtccccgt tctcgatcgc cggtcgctgg ctgccgacgc ccaggcccac cccggcctgg
     8461 gcgcaggcct cggccaggtt cgcgtttatc tgcttggcga gggggaatcc gccggtgatg
     8521 gccgtcacca ccagcggcat ctccaaccgc ttgccgaaca acgtcacgga ggtgtctatg
     8581 gcgtccatgt ccacctcagg cagggcccgg tggaccaggc ggacgtccga ccaatagtca
     8641 tagtcaggca cgaccttctc cttgagggtc acctcgatgt ggtccgattt gcgctgctcg
     8701 atctgcttca tgaaaccacc ctggaggaaa cgaccttcct tccgcggagg gccgcctcca
     8761 accttcccgg gaccttgccg ttgagcacca ggcactcccc ggccacctcg gccatgtcga
     8821 tcatgtactg gatcttggca tagatgctgc cggtcacatc cacgttcctc tgggtgcggg
     8881 gcagcgccgc cagcgtgccc cggtccaccc gctcgatgag cctggcgccc gggtcactgg
     8941 cgggatcggc ggtgagcacc ccgtccacgt cggttacgaa gatcgcccgg ctgggccgaa
     9001 gctccttggc caaggccatc atcagctggt cccccgagca gatgctcagg ccgcgcacct
     9061 cgtcccatac cacgtcgccg aaggacatcg gcaccatgcc caggtccagg tacctcagga
     9121 acgtggtcag ttccaggcgt tcgagcgccc cgtccctcat gaccgcgcag gccgccgggg
     9181 gcaggggcac cactggcagc cccgcatgga ccaggcaccg gcacatctcc aggtcgagct
     9241 cccggacgtc ggccatgacc tcggccgcgc ccctcagctg ggcatcctcg gtgatcccca
     9301 ggtgcagacg gtgctccttg gccaagatgt gtccgaagga cccggccccg tgcaccagca
     9361 caatcctctc cccggcggcc gctatctcgg aggcgaggcg gccggcggtc tcccggtcga
     9421 acctgcggta ctggcccttg tccgtgataa cgctcccgcc cagcttgacc agtatcatca
     9481 ggtccccgac gataatcggt ctatgggtat taatctttca atggcgtccg tacccttcgg
     9541 ccgcacttcg ggcagaacag gtccccctca tccagctcgg cggagcatcc ctcgcagttc
     9601 ctgccggtgg ccggctcccc gcacttcctg cagaacatat cccccggctg aataagggtg
     9661 gagcacttcc tgcacctctt ttccggtgga agccgctcca aggtgatgtc cttgggacgc
     9721 tccgcctcag ccggctcgcc gtcaaggagc ttgcgggtgc gaaccccctc ccgcaaggcc
     9781 tcgtcatatc gcttctcctc aaagcatcgc tccgcattcc gcaaatgctc ctcagcctcg
     9841 gaaacgttat ggccggcggc agagcgttcc tccatccggt cccggcacgc tctgatcaaa
     9901 aagcgggact ccatcatgtt aggctcaagc ttttttatct catgcaccgt cctgcgctcc
     9961 tcggccggag cgggggcctc gggcgcgggc ggggccattt cgatcaaggg cgcgttcatg
    10021 agcatctccc tggccttcat ggccaatccc ttggcggatg tgaaattgtt gcgatgatag
    10081 gcgccctctg ccagatttat tgtctgctcc gcctccttgg tatccctccc cctgccccga
    10141 agggcggagg cgatggcctt ggcgttggcg atggaattgt agacctcatc cttttccatc
    10201 gccctttgga tgtggtcctt gttgcgcgat cgcatgaagc gcagctcaaa gtagatgacc
    10261 accgctagca gcacaaacag aacgatcagc aggacctcgt tggcatccag caggaccacc
    10321 ttcggctttg agggacatta ggtcctttgt cgataataat cctatggtat agatggatgc
    10381 gcatacatgg gtatgatgcg aggtcttggc tctatcgata taaaagcaga ttggaattgg
    10441 aaagaggact agaatggctg acagcgttcc gggctttccg tgggctcgcg caccacagta
    10501 ccgcagggaa cgcgggcggg cttagcttct gggatcggac gagaccaggc gtatccccgc
    10561 cgctatggcc gtcagaccat cctacgccaa ggcaaacaat cccgttattt aaactttttt
    10621 acagcccggt ttttcaacgc cgatttaccg tccgtatatg tcatgagaag gttcgaacgt
    10681 gaataataac ggcccacgaa ttataaataa taatttatta tataatatat aatttataaa
    10741 tattatatga tcggtttcgt tattttgtca tcatatctga taccatagtg cgcgtgacca
    10801 tatttttcac gaatctcgtc aaagacctac catatgctgg ccaaaatcaa atccttaaaa
    10861 aaattgtaat aactattatt atcaataatg ataacaatac ttatataact cacatatagt
    10921 agcattatct tgattcaatc gactgacaga acattgacta cctaagggat tggaaagcat
    10981 cacacccgcc aaaaaggcac cgtctgtctc gggttgggtc gaaagttggg gggacacggg
    11041 catgagtgca agtagtggta cagtcaaacg tatcgatatc agcaaggtct tatccatact
    11101 gctcgcaata ttgatgatca gtgcggggtt cactaccttg ctcagcattg gtgtgaacac
    11161 cggtaccgag gtaagctata cttcggaaca cttcttgggg tatgatctgg aatcaggcac
    11221 ttgggtgaac gccgatcttg ggaaagcgta catggagggg gatttcgttt cctaccaatt
    11281 gcgcttggac agcgacagca agatctgggg cgcggaagag ttcagtatca aatacaactt
    11341 ctatcaagct gccaatgacg ccatattcgt ggacggtttc gacacctcct tggccaccgg
    11401 tttccagtat tccactggtg atattctcat ggacggggtc caagttccgt ccgccggctg
    11461 gggtacgcac atagccacgc cggaggccgg ggagtccgcg ggggtcactg atccgcagat
    11521 aatcaattac atggatgtct gggaggtcgg cgatccggat agcgaggccg atcccaacga
    11581 catgcgttac ttcactgtca agaacctacc gtgggaggat ttcacggatg ggtatgtcat
    11641 attgttcttc agggcgcacc tggcgctctc catcctctgg agggcggacc tggaacaggc
    11701 cctgccgcag gtcttggatg gtgatgagtt cgaggactgg acagggatca gcccgcacaa
    11761 cggctcatcc tacaccagcg gctcttcgag gcacttcacg cttgagtatc ctggcattgg
    11821 cgctaggacc atacccatcc ccatcacttc ctaccccacc gacataatca ccgggaggaa
    11881 gttcattaat gaggtcccat acgacggttg ggagatgaac atgataggcc ggctgagcct
    11941 ctggggcacc accggcgcgg attacgtata cataccctac aaccccccga cggtactgac
    12001 aggtaccgga ccgtgggccc tgggctactt cgccttcgac ggactcccga ggggcaacta
    12061 caccgtatgg gagagcgtgg tggacggtta catactggat gtcataggga ccagccccga
    12121 ccttcacctg tacgatcctg gaaagctgcc ctacttcgac gcggtcaccg agacctggtt
    12181 ggacaatccc gatgaggacc ttatggtcca cttcgacctt gagcgcggga ccaagatctc
    12241 ccccaaggtc cagaacatcg acttctacaa cctgcaggta ggggacatct ccgggtacaa
    12301 gattcacgac acagatgggg atggagtgcc cgatgaggga gagcccggcc tggccggatg
    12361 gaccatcgag ctgtgggagg aggacggtct gtccgccatg gatacgacga ccaccgacaa
    12421 cgatggttac ttcgagttca ctgacatcct cgtcggacaa tacatcgtca aggaggtcat
    12481 gcaggatggc tggttcaata ccaccccggt ggagatcagc gtctgcgtga gctgcggttc
    12541 ggatatcgac tccctgctct tcctgaacac aatgtactcg tccatctgcg ggtacaaggt
    12601 ggaggacatg aatgggaacg gggtccggga cgacagcgac ccggtgatct atgattggga
    12661 gatccagctc tacaaggatg acgtgttgat aaat
//
LOCUS       DAOSYN010000020        11680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_4679_length_11680_cov_4.347011, whole genome shotgun sequence.
ACCESSION   DAOSYN010000020 DAOSYN010000000
VERSION     DAOSYN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4679_length_11680_cov_4.347011"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..925)
                     /gene="pylSc"
                     /locus_tag="PLJ11_01785"
     CDS             complement(101..925)
                     /gene="pylSc"
                     /locus_tag="PLJ11_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043767 - pyrrolysyl-tRNA synthetase
                     activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolysine--tRNA(Pyl) ligase large subunit"
                     /protein_id="HOO03433.1"
                     /translation="MVMDFTVSQLQRLKELGVDGCYDRFDSAEDRNTSFEEMVRQAAR
                     THRKNLRDRCRQGQRHSVKDAEIKMVDTLVHHGFIECLTPIIIKKEGVYRMGITEEHP
                     LWKQIFWLNANECLRPMLAPNLYCIMRYLEKQIATPIRLFEVGPCFRKESQGCMHLEE
                     FTMINAVEIDPGCDRQERISELVRLMMEPFDVEYSLTTESSDVYGETIDVLVNGTEVA
                     SASYGPHKLDRDNGVVMPWTGVGMGLERLVQQINGESNIKRVGRSLVYLDGVRLDI"
     gene            1968..3461
                     /locus_tag="PLJ11_01790"
     CDS             1968..3461
                     /locus_tag="PLJ11_01790"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOO03434.1"
                     /translation="MGRSKEIFVLGSAISILQWDMETKMPPKGRGMRAEQLSALELLA
                     HKKITDPEVARMLEACEGDIASFDAVQRRNVHLMRRSYDESVRLPDALVAETAKHQTI
                     CNGSWKRAKAAKDFGLFRDDLAKMIDLRMQAADILQEVKGTRTQYDALLDLFEPGMTS
                     ERIAEVFKGLRDGLIRLIDKVRSSGTEPDMSVLARRVPISAQRRISSEAMAFIGFDTT
                     SPEAGGRLDETEHPFTTGSFDDVRITTHYYENRFFSSLFSVLHEGGHALYDQGIPREW
                     MYQPVGTPCSYGVHESQSRFVENIVGRSPEFLSYALPRFRKISPNAFKGLKGKDIIAA
                     VNAVVPSKVRIEADEVTYALHIIIRFEIERDIFAGKVTVDELPRLWDQKYSDYLGVKV
                     EDDAEGVLQDTHWSGGSFGYFPSYALGNIYSGMFLEKMEDDVPEWRESVRKGDFSPVL
                     RWLRANVHSRGDLYDPAELVKVVTGKEMDVAPFLGYLEGKMSRIYGF"
     gene            complement(3539..5455)
                     /locus_tag="PLJ11_01795"
     CDS             complement(3539..5455)
                     /locus_tag="PLJ11_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HOO03435.1"
                     /translation="MSAERILEEAREQVRKIVPSHIDISSIDFEGPVVVIYTKDMEAF
                     ASGNDMIRQLAQGLRRRVAIRPDPSTLANSADVEKRLREIIPEEAQITNISFEDDTGE
                     VTIEAIAPGLVIGKQGAVLNEIKKEIGWAPKVIRAPPIPSKTVYETRAYLREVQAERK
                     EFLRKVGRRLAQTKGEGENWIRMTCLGGYREVGRSATLLSTRESKVLIDCGMWPSKAD
                     PQPKPYFNAPEFLPFEQLDAVVITHAHMDHCGLLPVLFKYGYEGPVYCTPPTRELMSL
                     GLLDSIKVSYGEANKAEYEAKHVREVVKHCITLKYGETTDIAPDIRLTMQNAGHILGS
                     AVCHFHVGDGMYNVAFTGDMKYERSWLFNAATNKFPRLETLVIESTYGGPNDIQPSRV
                     DAATQLRGIIERAIMERKGKIVIPVFAVGRSQEVMLVIEELARTGKVDKVPVYLDGSI
                     WEATAIHTAYPEYLNSQLRTQIFQMGQNPFLSDMFKRVDSQEMRDKILHDIEPCIVLA
                     TSGMMNGGPVMEYVKAWASDPQVTLVFVGYQAEGTIGRKIQRGVKELTLSEKGKPITV
                     DFRMNVETVDGFSGHSDRRQLINYVASLDPKPERIVVGHGDEHKCLELASALYRKFNV
                     ETRAPMNLETIRFK"
     gene            complement(5506..6156)
                     /gene="psmB"
                     /locus_tag="PLJ11_01800"
     CDS             complement(5506..6156)
                     /gene="psmB"
                     /locus_tag="PLJ11_01800"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HOO03436.1"
                     /translation="MDNMKTGTTTIGIVNADGVVLASENRATMGNFIAHKETQKVFKI
                     DDNLGLTTAGLVGDAQILARYLRAEVELYKLKRASPMTVRSAATLTANILRRGGGAYG
                     YYYVGLIVGGYDKEGGHVFSLDAAGGSIPDHYISVGSGSPYAYGVLEDHYKKDIALDD
                     AINLAIRALNAAMKRDSASGDGMDIATITKDGYVEVSKEEILKRASRMGISVPRPN"
     gene            complement(6266..6811)
                     /locus_tag="PLJ11_01805"
     CDS             complement(6266..6811)
                     /locus_tag="PLJ11_01805"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOO03437.1"
                     /translation="MRIVAAKRFMLLIEAVLLTILMAVVLAPLTLPEGSATDLDGHIG
                     RVDNWERIVELPGPQRAVYLLGDVNCHQQAARSYELNGNQMPFCARDIGLLAGATMGL
                     AAFILLGRRPSWPWLLPFLVPLAMDGVLQAMTSYESTNAVRTATGAIAGLAVGYAIGA
                     LVQGYFAVPGHVRGERDDGHR"
     gene            6892..7326
                     /locus_tag="PLJ11_01810"
     CDS             6892..7326
                     /locus_tag="PLJ11_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HOO03438.1"
                     /translation="MPELPEPLRRLADFHGHLGPYVLLGYRMGLVARKYFPGKFYATL
                     FTGSEPPVSCLIDGVQFSSQCTLGKGNLNVKEGGRPMVRFYDGIRSLEMSPLETVQRR
                     IAMVSTYEAGERLALELHELSDLELLIIREGGTEASDRIVKL"
     gene            complement(7280..8977)
                     /locus_tag="PLJ11_01815"
     CDS             complement(7280..8977)
                     /locus_tag="PLJ11_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03439.1"
                     /translation="MALSFRLPPLMREVPPSMRRTFALKLEPGMAESMVRRGEAPGDI
                     EFAAFNAFLWTALLALPLALSSLFLLPYPYTMLFIGLFIAPLLLPMLVLAGPSKRFAA
                     EQRSYLIDTPVVIGAMGMSMNRSASLERAVEMGSRSGNGALQSSLASVAWKALTGEVQ
                     DLRRGLSAWTTGLGACNEGLRRSLQLVMAAEEEGGEEGRHRLLDRANSLALEWTREVC
                     ERYVGSLSFPVMLVFSFGVLAPVMLFSLVPLMGMEAGTASFALDLRSLALLLLVLVPA
                     FTLGYIRSMAGRNPLRTEAKAPVHRGPWMFLAFLSAPAAALGMLLTGSLPLAAIAALA
                     ILLAALIASGGRAKKTDRDREASFVDGLYRLGNAMLGGQDLDAAFEEVARTEQGAFRS
                     WALRMVHSLRTDRVSLARAVRDDPELREWSPVLYQNYVAVMECAEEDHRGAGKLAVNL
                     AQSLGDLTRTKQKVRENLRPVVDMMSATSVVFAPAIIGLTSGIMGLIGGDTSGIMALA
                     AVYVVELAFVVNYFLVSLDEGRGDRGGYRSYGTRGVLALGAFLIASLCGQTLLFRLL"
     gene            complement(8977..10524)
                     /locus_tag="PLJ11_01820"
     CDS             complement(8977..10524)
                     /locus_tag="PLJ11_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HOO03440.1"
                     /translation="MKPPISGYWIERERPVGWKVLERTEVLGGAIELIADPTGLRHRY
                     QLLPWEHSIDRSTGALLAKAVAKVRSEPPSSMSETEAELRAHVLSVLRSMAFPMAIED
                     EFTDAVMRNTLGLGVLDVILRDPRVEDVFVDAPCQQTRVYLTLNQSGPGHVFGRCETN
                     VIVSEGEMGALVGRLKYRSGRPFSRSTPVLETDLGDSRVRATALCPPLSPDGLALALR
                     RRSDLPWTLLRLAEHGAMDEVAAGLLSFLIDGRASILVCGARGSGKSSLLSALLFEFP
                     LDQRVLVIEDTREVPVGALQALGFKVQSMLVGPAQGKDGAGSAEEALRVSLRLGESAI
                     VMGEVRGKEAITLYEGMRTGKAGSSVLGTIHGDGAREVHDRVVNELGIPEASFASTDV
                     IVTMGLIRPGGGLGQVRRVTEVAEVVRTPDGLQFLPMLSYDGRYHLSERFMPLIARIA
                     SGWGMGYEEGLANLNRRIELRRVLLDMGRGDPSYLSPASILAANEYLWQGNGGSAADF
                     ISYMGER"
     gene            complement(10584..11165)
                     /locus_tag="PLJ11_01825"
     CDS             complement(10584..11165)
                     /locus_tag="PLJ11_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03441.1"
                     /translation="MERSHLPCSLGKKMAKVVMMPKGAGVPITPSVERGGCRFQVQGR
                     DVVFDCNGCPLAADVPSEGCLPSIRAALEAHQETMGIIFRGPEDVWVRSDGLEPLRSL
                     MAAEKAREGLRKALRSLPCPRPISAERIERFLEKAVAGTSDQFCQGRGEECLTCIERQ
                     QEAIGSMRSMSGKARKALAADRFRITEVRGGGR"
     gene            11230..>11680
                     /locus_tag="PLJ11_01830"
     CDS             11230..>11680
                     /locus_tag="PLJ11_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOO03442.1"
                     /translation="MELLAPAGTEEALRAAVAGGADAVYLGGKSFGARHFAGNFSDRQ
                     LAGAIELAHAHDMRVYVTVNTLIKEAEMADALAFVGMLDTLGADAVIVQDRGLLPLIK
                     DRFSIPVHASTQMGIHSLAGAAWAQEQGAERVILARELGLEQVQRIAE"
BASE COUNT         2388 a         3606 c         3308 g         2378 t
ORIGIN      
        1 tccatcaggt tcggcccttc cccggccaat gcccgatcga ggatatcgtc aagttcaaaa
       61 gggggggtcg accaacggtc ctccgacaat gttaacgggt tcatatgtct aacctcaccc
      121 catcaaggta gaccaggctt ctgcccactc gcttaatgtt gctctcgcca ttgatctgct
      181 ggaccagcct ctccaacccc atgcccaccc ccgtccacgg catcacgacc ccattgtccc
      241 tgtccaattt gtgggggcca taggaggcgg aggccacttc cgttccgttt acaaggacat
      301 cgatggtctc cccgtacacg tccgatgatt ccgtcgtaag gctatattcg acatcgaacg
      361 gctccatcat taggcgcact agctccgaga tacgctcttg acggtcgcag cccggatcga
      421 tctcgaccgc attgatcatt gtgaactcct ctaggtgcat gcatccctgc gattccttcc
      481 tgaagcacgg tcccacctcg aacaatcgga tgggagtggc gatctgcttc tccaggtagc
      541 gcattataca atacaagtta ggggcgagca tgggcctgag gcattcattg gcattcaacc
      601 aaaagatctg cttccacaga gggtgctcct cagttatgcc catgcggtac acaccctcct
      661 tttttattat tattggggtg aggcattcga tgaaaccatg atgtacaagg gtgtcaacca
      721 tcttgatctc cgcgtccttg accgaatgcc gctgtccttg cctacatcgg tccctgaggt
      781 tcttcctgtg cgtccttgcg gcctgcctca ccatctcctc aaagctggtg ttccggtcct
      841 cggctgaatc aaatcggtcg tagcacccgt ctactcccag ctccttgagc cgctgtaact
      901 gtgacaccgt gaaatccatt accatttcca attcccctta ttttccctta ttatatgtgg
      961 cggatgtggc ggagggtccg gaggttcaat ccggctcggt tggctttaga ggcctcatgg
     1021 tcgcggaccg atcgtcctcg gcggtccctc ggttcgcatg cctttccact aagccgcatt
     1081 ttccttcccc tgatttccta tctccttagt aggctattta aatattttgt taaggatatt
     1141 atatataaaa atcacaatat acaaattgcg ttttttaata taatattata tttatatata
     1201 taatagatta tagtatattt tactgctggt catatttttt aaattttatt gcttatcaaa
     1261 atatatctaa atttaaatta tataatttaa taattatata atatattata ttgagttttg
     1321 ttgtttttta attggctata aatttagcat tttaacatac aaatgaataa taatatactt
     1381 aattcataaa caaatatcta gaataatttt tattgataat aaatatattg tacattaaat
     1441 tatataataa taggaacgga tcgttgaaat tatgcaggtc ttaaacaaaa cctcatatgc
     1501 gatcaaaaga ggtgaaaaaa tacaaaaata taaatactat aaatttcaat gatatacacc
     1561 attcaaaaat ttatttatat tactatgaaa aacaaaattc gttctcataa aaagaataaa
     1621 taatatgaat ggttaaaata attattgaaa tcccttggcc caaggaacgc acctattgga
     1681 tcggaattga gctaaaaaca actttaaaaa tggcacttct tatgcggatg atctattata
     1741 tattaatata aacaaaaaaa tgggattcga ttaaaataaa catctaatta ttaattataa
     1801 tattgttgta aaggaccata tgcggatccc ccgattcaaa gatttgaaca cacctaggtc
     1861 gccaattcga tcgacacgcg atcaaataac aaaggttatt tcattcattc ccttaccctc
     1921 cctcgattca catggctgtt gatgatcatg gtccatacgc ccgcctgatg gggcgttcca
     1981 aggagatatt cgtcctgggc tcggcgatca gcattctaca gtgggacatg gagaccaaga
     2041 tgcccccgaa gggccgaggc atgcgggccg agcagctgtc cgccctggaa ctactggctc
     2101 ataagaagat aactgatccg gaggtcgccc gcatgctcga ggcatgcgaa ggggacattg
     2161 cttcgttcga cgcggtgcag aggaggaacg tccacctgat gcgcaggagc tacgacgaga
     2221 gcgttcgcct tcccgacgcc cttgtcgccg agaccgccaa gcaccagacc atctgcaacg
     2281 gctcttggaa gagggccaag gcggccaagg acttcgggtt gttccgggat gacctggcca
     2341 agatgatcga cctcaggatg caggccgcgg acatcctcca ggaggtcaag gggaccagga
     2401 cccagtacga cgccctcctg gacctgttcg agcctggaat gacctccgag cgcatcgccg
     2461 aggtattcaa ggggctgcgc gacgggctca tcaggctcat agacaaagta cggtcctcgg
     2521 ggaccgagcc cgacatgtcg gtgctcgccc gcagggtacc gatctccgcc cagcgtagga
     2581 tctcctccga ggcgatggcc ttcatagggt ttgatacaac gagcccagag gcaggcgggc
     2641 gcctagacga gacggaacat ccattcacca ctggcagctt cgatgacgtc cgcatcacca
     2701 cgcactacta cgagaaccgg ttcttctcct cgctcttcag cgtgctgcac gaaggcggcc
     2761 atgccctgta cgatcagggc atcccaaggg aatggatgta ccagccagtg gggacgccct
     2821 gctcgtacgg cgtccacgag tcccagtccc gcttcgtgga gaacatcgtg ggccgaagcc
     2881 cggagttcct gagctacgcc ctgccccgct tccgcaaaat atccccaaac gcgttcaagg
     2941 ggttgaaggg caaggatatc atcgcggccg tcaacgcggt agtgccctcc aaggtccgga
     3001 tcgaggccga cgaggtcacg tacgcgctcc acatcataat ccggttcgag atcgaaaggg
     3061 acatctttgc cggcaaggtc actgtggacg agctgccccg actttgggac cagaaatatt
     3121 ccgactacct aggcgtgaag gtcgaggacg acgccgaagg ggtgctgcag gacacccact
     3181 ggtccggagg aagcttcggc tacttcccct cctacgccct gggcaacatc tattccggca
     3241 tgttcctcga aaaaatggaa gatgatgtgc ctgaatggcg cgagagcgtc cgaaagggcg
     3301 acttctctcc cgtgctgagg tggctgcgtg cgaacgtgca ctccagggga gacctgtatg
     3361 acccggcgga actggtcaag gtggtcacag gcaaggagat ggatgtggcg cccttccttg
     3421 ggtacctgga agggaagatg tccaggatct acggcttcta agggatcggg accgcctccc
     3481 tccttccctt ttggccctct tcccctacat cacagtagaa aaggggcgaa cggaggcctc
     3541 acttgaagcg aatggtctcc aggttcatcg gggcgcgggt ctccacattg aacttgcggt
     3601 acagggctga tgccagctcc aggcacttgt gctcatctcc gtgtccgacg acgatgcgct
     3661 ccggcttggg gtcgaggctc gccacgtaat tgatcagctg cctgcggtcc gagtgccccg
     3721 agaacccgtc caccgtctcc acgttcatgc ggaagtcgac ggttatcggt ttgccctttt
     3781 cggacagggt gagctccttc acccccctct ggatcttgcg gccgatggtg ccctcggcct
     3841 gatacccgac gaacaccagg gtgacctggg ggtcgctggc ccaggccttg acgtactcca
     3901 tgaccgggcc gccgttcatc atgccgctcg tcgccaggac gatgcacggc tcgatatcat
     3961 gcaggatctt gtcccgcatc tcctgcgagt ccaccctctt gaacatatcg ctcaggaagg
     4021 ggttctgccc catctggaag atctgggtgc gcaattggct gttcaggtac tccggatagg
     4081 cggtatggat ggccgtcgcc tcccagatgc tgccgtcaag gtagacgggc accttgtcca
     4141 ccttgccggt ccgggcaagc tcctctatca ccagcatgac ctcctgtgag cgcccgacgg
     4201 cgaacaccgg gatgacgatc ttccccttgc gctccatgat ggcgcgctcg atgatcccgc
     4261 gcagctgtgt cgcggcgtcc accctgctgg gctggatgtc gttcggcccc ccgtaggtcg
     4321 actcgatgac aagcgtttcc aggcgcggga acttgttggt ggcggcgttg aacagccagc
     4381 tccgctcata cttcatgtcg ccagtgaagg cgacgttgta catcccgtcc ccgacgtgga
     4441 agtggcagac ggccgaccct aggatgtgcc ccgcgttctg catcgttagt cgtatgtccg
     4501 gggcgatgtc tgtggtctcc ccgtacttca gggtgatgca gtgcttcacc acctcccgga
     4561 cgtgcttcgc ctcgtactcc gccttgttcg cctccccata ggacaccttg atggagtcca
     4621 ggaggccgag ggacatcagc tccctggtgg gcggggtgca atagacaggc ccctcgtacc
     4681 cgtacttgaa cagcacgggc aacaggccgc agtggtccat gtgggcatgg gtgatgacca
     4741 ccgcgtccag ctgctcgaac gggaggaact cgggggcgtt gaagtagggc tttggctgcg
     4801 ggtctgcctt ggagggccac atcccgcagt cgatcaggac cttgctctcc ctcgtcgaga
     4861 gcagcgtggc ggaccgcccc acctcccgat aaccgcctaa acaggtcatg cggatccagt
     4921 tctccccttc gcccttggtc tgcgccagtc gccttccgac cttgcggagg aactccttgc
     4981 gctcggcctg cacctccctg aggtaggcgc gggtctcata caccgtcttc gatggaatgg
     5041 gcggggccct gatgaccttc ggcgcccagc cgatctcctt cttgatctcg ttgagcactg
     5101 ccccctgctt ccctatcacc agcccggggg ctatggcctc tatcgtcacc tcgccggtgt
     5161 cgtcctcgaa cgagatgttg gttatctgcg cctcctccgg gatgatctcc ctgagccgct
     5221 tctcgacatc agcggaattg gccaaggtgg aggggtctgg ccggatcgcc accctccgtc
     5281 tcaatccttg ggcgagctgg cggatcatgt cgttcccgga ggcgaaggcc tccatgtcct
     5341 tcgtgtatat gactacgacc gggccctcga aatcaatgga agatatgtcg atatgggacg
     5401 gtacgatctt ccgtacctgt tctcgggcct cctcgaggat cctttctgcg ctcatggcgt
     5461 tcactttacg ggaaatataa caggaaagat ggttggtaag gcgtttcagt tgggcctggg
     5521 gacgcttatc cccatgcgcg atgcccgttt caggatctct tccttggaca cctcgacgta
     5581 cccgtccttc gtgatggtcg ctatgtccat gccgtccccg ctcgcagagt ccctcttcat
     5641 cgccgcgttc aacgcacgga tcgccagatt gatggcgtca tccaaggcga tatccttttt
     5701 atagtgatcc tccaggaccc cgtaggcata gggcgagccg gagccgacgc ttatgtaatg
     5761 atcgggtatg gaaccgccgg ccgcgtccag ggagaagaca tgtccgcctt ccttgtcgta
     5821 cccgcccacg atgaggccga catagtaata gccgtaggca cctcctcccc tccgcaggat
     5881 gttggctgtc agggtagcag cggacctgac cgtcatcggc gaggcccgct tgagcttgta
     5941 gagctccacc tcggcccgca gataacgggc caggatctgg gcgtccccaa ccaggccagc
     6001 ggtggtcagg cctaggttgt cgtcgatctt gaagaccttc tgggtttcct tgtgggcgat
     6061 gaagttgccc atggtggccc tgttctcgga cgccaggacc actccgtcag cattgactat
     6121 ccctatagtg gtagtaccag tcttcatgtt gtccatatga atcgagtcct ccaaaacctc
     6181 gtaaaaacga attagatgga acgactggag cctcaatcaa cactattata tataagtgtt
     6241 ttccaagaac gcagcggccg acggctcatc gatggccatc gtcccgctcc ccacgtacgt
     6301 gcccgggaac ggcgaaatag ccctgtacca gggcccctat cgcatatccc acggcaaggc
     6361 cggcgatcgc cccggtggct gtacggacgg cgttggtcga ctcgtacgac gtcattgcct
     6421 gaaggacccc gtccatggcc aagggcacca ggaagggcaa gagccatggc cacgaaggcc
     6481 ttcgcccgag aaggatgaac gctgccagtc ccatcgtcgc acctgccaaa agcccgatgt
     6541 ccctggcgca gaagggcatc tggttcccat tgagttcata ggagcgcgca gcctgctggt
     6601 ggcagttcac gtcccccaag agatagactg ccctctgcgg accggggagt tccactatcc
     6661 tttcccagtt gtcaacccgc ccgatatgac cgtctaggtc ggtggcgctc ccctccggta
     6721 gggtcagagg ggcaagcacc acggccatca ggatcgtcag gaggacggct tcgatgagca
     6781 gcatgaagcg cttggcggcc actatcctca ttggaggcgc ctaccgcgct ttgactataa
     6841 atcgattgat gggggcgtgg atttatattg ggcgaacccc tccgttccct catgcccgag
     6901 cttcccgagc ccctccgaag gcttgccgac tttcatggac atctcggtcc ctacgtcctt
     6961 ctgggatacc ggatggggct ggtggccaga aaatactttc cagggaagtt ctacgcgacc
     7021 ctgttcacgg gcagcgagcc cccggtctca tgcctgattg acggggtgca gttctcctcg
     7081 cagtgcacct tgggcaaagg gaaccttaac gtcaaggagg gcgggcggcc gatggtacgg
     7141 ttctatgacg ggatccgctc cttggagatg tcccccctgg aaacggtgca aaggcggatc
     7201 gccatggtca gcacctatga ggcgggggaa aggcttgcat tggagctgca tgaactctcc
     7261 gaccttgagc tcctgatcat cagagaaggc ggaacagaag cgtctgaccg catagtgaag
     7321 ctatgaggaa ggcccccagg gccaggaccc cgcgggtacc gtagctgcgg tatccgccgc
     7381 ggtcgcccct tccttcgtcc aagctgacaa ggaagtagtt caccacgaag gccagctcca
     7441 cgacgtacac ggccgccaag gccatgatcc cggaggtatc gccccctatg aggcccatta
     7501 tcccgctggt cagaccgatt atcgccgggg cgaacaccac gctcgtcgcg ctcatcatgt
     7561 ccaccacggg ccggaggttc tcccgcacct tctgtttcgt cctcgtcagg tccccgaggg
     7621 actgggccag attcaccgct agcttgccag cgccccggtg gtcctcttcc gcacattcca
     7681 tgaccgccac gtagttctgg tacagcaccg ggctccattc ccggagctca gggtcgtccc
     7741 ggaccgcgcg agcaagggag acgcgatcgg tgcggagcga atggaccatc cgtagcgccc
     7801 agctccggaa ggcgccttgc tcggtgcggg ccacctcctc gaaggccgca tcgaggtcct
     7861 ggcccccgag catcgcgttg cctaatcggt acagcccgtc cacgaaggac gcttcccggt
     7921 cccggtcggt cttcttcgcc cgaccgccgg aggcgatgag ggcggcgagc aggatggcaa
     7981 gcgcggcaat ggccgccagg ggcaaggagc cggtgaggag catccccaag gccgccgccg
     8041 gggccgatag gaacgccagg aacatccatg gcccccggtg caccggcgcc ttggcctcgg
     8101 tgcgcaacgg gttccgaccg gccattgagc ggatgtagcc cagggtgaac gctggcacca
     8161 gcacaagcag gaggagggcg agggaacgga ggtccagggc gaagctcgct gtccccgcct
     8221 ccatccccat gagcggaaca agggagaaga gcatgacagg ggccagcact ccgaacgaga
     8281 acacgagcat gaccgggaag gaaagggagc ctacgtaccg ttcgcagacc tccctcgtcc
     8341 attccaaggc aagggagttc gcccggtcca acagacggtg ccgtccttcc tccccgccct
     8401 cctcctcggc ggccatgacc agttgcaacg acctccgcag cccctcattg caggcgccta
     8461 ggcccgtggt ccatgccgaa agccccctgc gcaggtcttg cacctctcca gtaagggcct
     8521 tccacgccac ggatgccaag gaggattgaa gcgctccgtt ccctgagcgg gagcccatct
     8581 ctacagccct ctccagggac gctgagcggt tcatgctcat ccccatggcc ccgatcacca
     8641 ccggcgtatc gatgaggtat gagcgctgct cggccgcgaa cctctttgaa ggacccgcaa
     8701 ggaccagcat tggaaggagc aggggagcga tgaaaaggcc gatgaagagc atggtgtatg
     8761 gataggggag cagaaagagg gaggagaggg cgagcgggag ggcgagcagc gccgtccata
     8821 ggaatgcgtt aaacgcggcg aactctatat cccccggggc ttccccccgc cggaccatgg
     8881 actcggccat cccgggctcc agcttgaggg cgaacgtcct ccgcatcgac ggggggacct
     8941 cccgcatgag cggggggagc ctgaaagaca gcgccatcac ctctccccca tatatgagat
     9001 gaaatcggcc gcagagcccc cgttcccctg ccagaggtac tcgttcgccg caaggatgct
     9061 ggccggtgaa aggtatgaag ggtcccccct tcccatgtcc agcaacaccc tccgcagctc
     9121 gatccttctg ttgaggttcg ccaagccctc ctcgtagccc atcccccacc cggaggctat
     9181 gcgggcgatg aggggcatga agcgctcgct gagatgataa cgcccatcgt agctgagcat
     9241 gggcaggaat tggagcccgt cgggcgtgcg tacgacctcc gccacctccg ttaccctcct
     9301 cacttgtcca agcccccctc caggccttat tagccccatg gtcacgatga cgtccgtgga
     9361 ggcgaaggag gcctcgggga tcccgagctc gttcacgacc cggtcatgga cctccctggc
     9421 gccatcccca tggatcgttc ctagcaccga ggaccccgcc ttgcccgtgc gcatcccttc
     9481 gtacaaggtg atcgcctcct tgccccgcac ctcgcccatc acgatcgccg actcgccgag
     9541 gcgcagggag acccgcaacg cctcctcggc cgaccctgcc ccgtcctttc cctgggcagg
     9601 gccgaccagc atggactgca ccttgaaccc caaggcctgc agggcgccta ccggcacctc
     9661 ccgggtgtcc tcgatgacaa ggaccctctg gtccaatggg aattcaaaga gcagggcgga
     9721 taggagggat gatttgccgg agccccgggc cccgcagacc aggatcgatg cccttccgtc
     9781 gatcaggaag gagagcaggc cagcggccac ctcgtccatg gccccatgct cggccagccg
     9841 cagcagggtc cagggaaggt cggacctccg gcgcagtgcc agggccaggc cgtccgggct
     9901 caggggaggg cagagggcgg tcgccctcac ccgggaatcc ccaaggtccg tctcaagcac
     9961 cggggtggag cgggagaagg ggcgccccga ccggtacttc agtcggccga cgagcgcgcc
    10021 catctccccc tccgagacga tgacgttggt ctcacaccgt ccgaacacat ggcctggccc
    10081 ggattgattg agggtcaggt atacccgtgt ctgttggcac ggggcgtcca cgaacacgtc
    10141 ctccacccgg gggtcgcgca ggatgacgtc aagcacccca agtcccaggg tgttcctcat
    10201 caccgcgtcc gtgaactcat cctcaatggc catgggaaag gccatcgagc gcaagaccga
    10261 aaggacatgc gccctgagct cggcctccgt ctcgctcatg gacgatggag gctccgacct
    10321 gaccttggcc accgccttgg cgagcagggc gccagtgctc cgatcgatgg aatgctccca
    10381 tggcagcagc tgataccgat gacgaagccc ggtggggtcc gcgatgagtt cgatcgcccc
    10441 cccgagcacc tcggtccgtt ccaggacctt ccatcccact ggtcgctccc gttctatcca
    10501 atacccggag atggggggct tcaccaagcg tgcccggcga agcgcaggaa gcgcatctgg
    10561 aagggtgagt ggacgggaag ggctcatcgt cctcccccgc ggacctcggt gatccggaaa
    10621 cggtcggcag ccaacgcctt cctcgccttt ccgctcatgg agcgcatcga accgatcgcc
    10681 tcctgctgtc tctcgatgca ggtgaggcat tcctcccccc gcccctggca gaactggtcg
    10741 gaggtcccgg cgacggcctt ctccagaaag cgttcgatcc tctccgctga aatcggccgc
    10801 ggacagggaa gggaacggag ggccttccgc aacccttccc gcgccttctc tgccgccatc
    10861 agggagcgca gtggctccag accatcactc cttacccaga cgtcctcggg tcccctgaat
    10921 atgatcccca tcgtttcctg atgggcctca agggctgccc gtatgctggg aaggcacccc
    10981 tccgatggaa cgtcggccgc caatgggcaa ccgttgcaat cgaagacaac gtccctaccc
    11041 tgcacctgga aacgacagcc gcccctctcc actgaaggcg taatggggac gcccgcgccc
    11101 ttcggcatca tgaccacctt cgccattttc ttcccgaggg aacaggggag atgggagcgt
    11161 tccatgtaac ggctaaccgt acattacgtc gtcagggaaa ttaatatccg aaggaatgat
    11221 tccccgaaca tggagcttct agcccccgct ggaacggagg aggcgttgag ggcggccgtt
    11281 gccggagggg ccgatgcggt ctatctgggg ggaaagagct tcggtgcccg acatttcgcc
    11341 ggtaacttca gcgacaggca gctggcaggc gcgatcgagc tcgcgcatgc ccatgacatg
    11401 cgcgtctatg tgaccgtgaa cacgctcatc aaagaagcgg agatggcaga cgcactggcc
    11461 ttcgtgggga tgctggatac cctgggggca gacgcggtga tagtccagga ccgcggcctg
    11521 ctcccgctga tcaaggatcg gttctccatc ccggtgcacg cctccacgca gatgggcatc
    11581 catagcctag caggcgccgc ttgggcccag gaacaggggg cggagagggt gatcctcgcc
    11641 agggagctgg ggctggagca ggtccagcgc atcgccgagg
//
LOCUS       DAOSYN010000021        11673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_4687_length_11673_cov_4.471165, whole genome shotgun sequence.
ACCESSION   DAOSYN010000021 DAOSYN010000000
VERSION     DAOSYN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 11673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11673
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4687_length_11673_cov_4.471165"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="argG"
                     /locus_tag="PLJ11_01835"
     CDS             complement(<1..642)
                     /gene="argG"
                     /locus_tag="PLJ11_01835"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOO03443.1"
                     /translation="MAARSRNARDKVVLAYSGGLDTSVAIRWLQQKYDLDVIAVSVNV
                     GQPGDLQEAVDRALRIGAVKAYAFDATEEFVTEYIAPSLKANALYMGAYPLATSIARP
                     LIAKTMVEIAKKEGAKYIAHGCTAKGNDQVRFDVSIAALAPEIKIIAPMREWVMTREE
                     EIEYARQNDVPIKVKKECPYSTDENIWGRSCECGILEDAMAEPPQDAFEWTTDP"
     gene            896..1912
                     /gene="argC"
                     /locus_tag="PLJ11_01840"
     CDS             896..1912
                     /gene="argC"
                     /locus_tag="PLJ11_01840"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOO03444.1"
                     /translation="MIKAAVVGGSGYIGGEIVRLLSRHPRISLEAITSRQLAGQKVAD
                     AHPFLRGYVDLTFSESVDGQGLDVVFLATPHGASMNLVPGLMRSGAKVIDLSGDYRLK
                     DPQEYKRWYGHEHTDPENLGRAVYGMNELYWDEIREADLVANPGCYPTCSTLGLAPLF
                     ANHLVEGGVIIDAKSGTSGAGAEPTKATHHPNCGASVTPYKVGTHRHTPEIAQNLTRL
                     QGRLAEVVFTPHLIPVVRGMLCTMYLRLNEDLTKQELLGVYTEFFRRKRFVRLTEVPW
                     MQAVVGSNHCDVGLEVVGPRKVVVMSAIDNLVKGGAGQAVQNCNAMFGMPEEMGLDFP
                     GLGV"
     gene            1915..3141
                     /gene="argJ"
                     /locus_tag="PLJ11_01845"
     CDS             1915..3141
                     /gene="argJ"
                     /locus_tag="PLJ11_01845"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOO03445.1"
                     /translation="MDVLEGGITSPKGYKAAGVHCGIKKEKLDLAVIYSDVPAKCAIA
                     YTQNKVRAAPIEVMMKKDPRMLQAMVVNSGNANAITGAQGIRDAQQMVALTAQALGVK
                     DDLVGVASTGVISRFLPMDRVAEGIPKAVKALGRGPEADDMAARAILTTDTVKKEAAC
                     KVTLKDGTPVTIAGITKGSGMISPAMKVPHATTLSFMVTDAQLSGDFNRRWQDIMDVS
                     FNVISVDGDQSTNDMSVLMANGMAGGRPADNDPAFWEGVRYVAQQLAKKIVIDGEGAT
                     KLIEVIVRRAKSPVQARSAARGIITSSLVKTAIFGADPNFGRILAALGNSEAEFNLDN
                     VRLVLRSNGDSVTLFEDGSPKLIAGNEVEAAARKVLSHREVIIDLDLNNGHATGEAWG
                     CDLSYDYVRINAMYTT"
     gene            3194..4000
                     /gene="argB"
                     /locus_tag="PLJ11_01850"
     CDS             3194..4000
                     /gene="argB"
                     /locus_tag="PLJ11_01850"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOO03446.1"
                     /translation="MVKFGGSSIGGMDRIDAFAMQVAELKERGLVPVVLHGGGPEITD
                     EMKRCGLPVRKVAGLRITDDATLEIAKSVLSRINDRVVVSLRKAGLRSIGLAGSEVDT
                     LVARRMPPVLVNGPDGREEVDLGYVGEVVKVDPRAVTALCAKGFVPVIYSICADASGM
                     LMNVNADTAAAALAAGIGASDLVLVTEVPGVLRTIDDLSSTVPEIREADLGPLTSSGV
                     LRDGMIPKVEACFIALRGGVGTAHIICGTTPNAIVDQLFGGRNLGTRLTL"
     gene            4010..5191
                     /locus_tag="PLJ11_01855"
     CDS             4010..5191
                     /locus_tag="PLJ11_01855"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HOO03447.1"
                     /translation="MDSKQVAELSSYYLFQNYAREALCFSHGKGEYLWDLEGNRYIDY
                     VAGIAVNCLGHAHPELVKAICEQASRLIHVSNLYQVKEQADLGEALAAIVPAPLGRSF
                     FCNSGAEANEAALKLAVKHTGRPKVVAARNSFHGRTAATLSVTGQGKYQKGFEPLLSR
                     NVDFIGYNSVEELKAAVDHGTAALILEPVQGEGGVIPASQEFFRAARDLCTDTGALML
                     VDEVQTGMGRTGEWFGFQHYGVVPDVISLAKGLGGGVPIGAIITSPDIAKAFTPGVHG
                     TTFGGNPLACAAASAVIRVLRQERLVERAAELGAEWKRELVRTVQGHEGVTEVRGMGL
                     MLGVELGDLAKGFQAYALARRLLVNVAGGRTVRLVPPLIISKGSIQELDAALSSYLHG
                     N"
     gene            5369..6055
                     /locus_tag="PLJ11_01860"
     CDS             5369..6055
                     /locus_tag="PLJ11_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOO03448.1"
                     /translation="MKEPPQKESVAERTRAYIDAHPSVKDCIAKDLINYSSLARMIMK
                     DLDIKNEEAVMIACRRYAQKLARKDHEGEILSILADSRLEVKTKICIVTASNDWAVLQ
                     RLERLFAKLINQKALMQVIQGAQAITIIADEKLRSEVVTEVGEGNVLKVREDLVEITV
                     KSPQRIGETSGVFSYLASNLAEQGVNIVETVSCYTDTIFIVHEKDMIPAYSLLSNLLE
                     SSERVTEARH"
     gene            6101..7450
                     /locus_tag="PLJ11_01865"
     CDS             6101..7450
                     /locus_tag="PLJ11_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HOO03449.1"
                     /translation="MDQARERAPRVLTDIPGPRARGIVELDDRYLATATKSAPLAVER
                     AEGEKVWDVDGNLFLDFSSGVAVMNIGHCHPSVVRAVQEQAAKVMHFAGTDYYYDVQA
                     RLAERLARIAPGRGEKKVFLSNSGAESIEAAMKLARWGSGRRQFLAFMGAFHGRTFGA
                     LSLTCSKRVQRARYFPMVPGVTHIPYAYCYRCPYHQEYPSCGLWCAKVLDEVYLDALV
                     PPDEVAALFFEPVQGEGGYIVPPKAFLPEIARICRSHGILLVDDEVQAGMGRTGRMWA
                     AEHFGVEPDITCMSKALGSGMPIAATVFRKELDWGVPGAHSNTFGGNCVACASSLATL
                     DVIEREGVVEQAERKGAVMERRLLEIKDRFEAVGDVRGLGMMRALELVEDRRTKAPAA
                     KLRNAILANMLRKGVIALGCGRSSIRLVPPLTIAEDNLDTGMRLLEESIAEAVRERA"
     gene            7447..7800
                     /locus_tag="PLJ11_01870"
     CDS             7447..7800
                     /locus_tag="PLJ11_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO03450.1"
                     /translation="MRLFPRSGHRKVQGDGYLKLVMLEGKDIGVSGTLLQEVQFTAGQ
                     RVRMHFHQATREVFYCLEGPAPFVINGEKVVMEEGDCLVCEPGDVHGNPVIGNDFRIL
                     VLKVGLREHDTVWLD"
     gene            complement(8010..8082)
                     /locus_tag="PLJ11_01875"
     tRNA            complement(8010..8082)
                     /locus_tag="PLJ11_01875"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8047..8049),aa:Thr,seq:tgt)
     gene            8252..8812
                     /locus_tag="PLJ11_01880"
     CDS             8252..8812
                     /locus_tag="PLJ11_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOO03451.1"
                     /translation="MDRTGNPAPLGLLGFGMTTVMLNLHNADLIPLSSVILSLGIFYG
                     GLAQLLVGLMEWRKGNTFGMAAFTSYGLFWLTLVGIWTIPSLGLGEPTDSTAMGCFLA
                     MWGIYTLFFFIGTAKSNRATQFVFGSLAVLFFALALGDLTLIKEITVTAGYIGLVCGA
                     SAIYTGLATVVNEANGRAIFPLGDRR"
     gene            8893..10053
                     /locus_tag="PLJ11_01885"
     CDS             8893..10053
                     /locus_tag="PLJ11_01885"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOO03452.1"
                     /translation="MDVGLAVIGVQVTSMDKESWKFLQELCDIPGPSGFEAAATRLMK
                     DYAGNYCDRVFTDKLGNTMFEKVGQKDGPTVLLAGHVDECGFIITGVNPQGYLAFSQL
                     GGWFDQVLLGQRVLVHTRKGVLNGVIACKPPHLMEPEEAKKVVTKDKMFIDVGACNKE
                     EAQAMGVRLGDAVTPDSRMFTVEKKAYKDGKPIGKRTIVFGRALDNRLGAFAAVELLK
                     AIKKGGVKHPNRLVAAATVQEEVGLRGAKTVAASTDPDVAIVLDVDIAGDVPGIDPLQ
                     APARMGEGVSITVYDGTMIPNQPLKELVISICERKKIPYQLAYVIRGGTDGGSIHTSL
                     TGVPTVVIGVPTRHIHSHVGLFDTADLKACMKLIVELAKALDRRTVDSFTAL"
     gene            10912..>11673
                     /locus_tag="PLJ11_01890"
     CDS             10912..>11673
                     /locus_tag="PLJ11_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO03453.1"
                     /translation="MTDGPTTAKVSKGDIVHVDYDAYIAESGMLFDTTNADKAKEAGI
                     FNENLTYKPMPVLVGGGRVFPGLDEALAEAKVGERTTVEIPPEKAAGARDPKLVENIP
                     LREFFRQDIRPEVGMEVNIKDRVGTVVAVTNRMVRVDFNRRFAGAALRYEFTVLDKVE
                     DAVAKALAICEMDYETSEGFRAVVSPDKVTLYLPDVCKYDPKWLLSKYKVVADLREVF
                     GRVDVELVEEYLKKEEKPTEEKPTEAQAKAAEEKAP"
BASE COUNT         2362 a         3496 c         3815 g         2000 t
ORIGIN      
        1 cggatccgtc gtccattcga aggcgtcctg cggcggctcg gccatggcat cctcgaggat
       61 gccgcactcg cagctccgtc cccatatgtt ctcgtccgtg ctgtacgggc actccttctt
      121 gaccttgatg gggacatcgt tctgcctggc atactcgatc tcctcctccc tggtcatgac
      181 ccattccctc atcggggcga tgattttgat ctcaggcgca agggcggcga tgctgacatc
      241 gaacctgacc tggtcattgc ccttggcggt gcacccatgg gctatgtact tcgccccctc
      301 cttcttggct atctccacca tggtcttggc gatgagcgga cgggcaatcg atgtggcaag
      361 gggatacgcc cccatataca gggcgttggc cttgagggaa ggcgcgatgt actcggtcac
      421 gaactcctcg gtggcgtcga aggcgtacgc cttcaccgct ccgatcctca aggcccggtc
      481 caccgcctcc tgaaggtccc caggctgacc aacgttgacg cttaccgcga ttacatccaa
      541 atcatacttc tgttgcaacc agcggatggc cacggaggtg tccaaaccgc ccgagtatgc
      601 caggacaacc ttgtcacgag cgttcctaga gcgagctgcc atgtgaatcg ggggcgatga
      661 ggcgagcatc tatattaatc tgaagcgccg acgatgcgac attggttcga ccaagtgttc
      721 ggaatgggac agctccgggc ggagggaagg atgggaatgg atggtgcggg atcgaaaaag
      781 gtgtccatgc aaggggcggg gggacctaaa atgtcccgtt tgagtcgcaa tacttaataa
      841 agatactctt aattcgggac attcccatgg gaattaccca cagcgggggc gcgggatgat
      901 caaggcagcg gtggtcgggg gatccgggta catagggggc gaaatagtgc gccttctgag
      961 ccgccatccc aggatctccc tcgaggcgat cacgtcccga cagctggctg gacagaaggt
     1021 ggcggatgcc catccctttc tccgcggata cgtggacctc accttcagcg agtccgtgga
     1081 cgggcagggg ctggacgtgg tcttcctggc cacaccgcat ggggcgtcca tgaacctcgt
     1141 cccggggctg atgcgcagcg gcgccaaagt aatcgaccta agcggcgact atcggctcaa
     1201 ggaccctcag gagtacaagc ggtggtacgg gcacgagcac acggaccccg agaacctcgg
     1261 cagggcggtc tatggcatga acgaactcta ctgggacgag atcagggagg ccgacctcgt
     1321 ggccaatccc ggctgctatc ccacatgttc cacgctgggg ctcgcccccc tcttcgcgaa
     1381 ccacctagtg gagggcgggg tcataatcga tgccaagagc ggaacgtcgg gggcgggggc
     1441 ggagccgacg aaggccactc accatcccaa ctgcggggcc agcgtcaccc cgtacaaggt
     1501 ggggacccac cgccacaccc cggagatcgc ccagaacctc accaggctcc agggcaggct
     1561 ggccgaggtg gtgttcacac cgcatctcat tccagtggta cgagggatgc tctgcaccat
     1621 gtacctcagg ctcaacgagg acctcaccaa gcaagaactg ctaggggtct ataccgaatt
     1681 cttccgtagg aagcgcttcg tgcgcctgac ggaggtgccg tggatgcagg ccgtggtcgg
     1741 ctccaaccat tgcgatgtgg gattggaggt cgtgggcccc aggaaggtgg tggtcatgag
     1801 cgcgatcgac aacctcgtta aagggggggc cggacaggcg gtgcagaact gcaacgccat
     1861 gttcggaatg cctgaggaga tgggattgga ttttccaggg ttgggggttt gaaaatggat
     1921 gttcttgagg gaggaattac ctcgccgaag gggtacaagg cggcaggggt tcactgcggg
     1981 ataaagaagg aaaagcttga cctggccgtc atctattccg atgtgccggc caaatgcgcc
     2041 attgcataca cccagaacaa ggtcagggcg gcgcccatcg aggtcatgat gaagaaggac
     2101 cccaggatgc tgcaggccat ggtggtcaac agcgggaacg ccaacgccat caccggcgcg
     2161 caggggatcc gcgacgccca gcagatggtg gcccttaccg cccaggccct gggagtaaag
     2221 gacgacctag tgggcgtggc ctccaccggc gtcatatcca ggtttctccc gatggacagg
     2281 gtcgcggaag ggatacccaa ggccgtcaag gccctgggga ggggtcccga ggcggacgac
     2341 atggcggcca gggccatatt gacaaccgac acggtgaaga aggaggccgc ctgcaaggtc
     2401 acattgaagg atggcacgcc cgtcaccatc gctggcatca ccaaggggag cgggatgata
     2461 tcgcccgcaa tgaaggtgcc ccacgccacc accctcagct tcatggtcac cgatgctcag
     2521 ctgagcgggg acttcaaccg tcgctggcag gacataatgg acgtcagctt caacgtgata
     2581 agcgtggacg gggaccagag caccaacgac atgtcggtcc tcatggccaa tggaatggcc
     2641 ggcggcaggc cggcggacaa cgaccctgcc ttctgggagg gagttaggta cgtggcgcag
     2701 cagctggcca agaagatagt catagacggc gagggcgcca cgaagctcat cgaggtaata
     2761 gtgcggaggg cgaagagccc cgtccaggcc aggtctgccg cccggggcat catcacctcc
     2821 tcgctggtca agacggcaat cttcggggca gaccccaact tcggccgcat cctggcggcc
     2881 ctgggcaatt ccgaggccga gttcaacctg gacaacgtca ggctcgtgct ccggtccaac
     2941 ggggactcgg tcaccctgtt cgaggacggt tcgccgaagc tcatcgcggg gaacgaggtc
     3001 gaggcggccg cgcgcaaagt gctctcgcac cgggaggtca tcatcgacct ggacctcaac
     3061 aacggccatg ccaccgggga ggcctggggc tgcgacctca gctacgacta cgtcaggatc
     3121 aacgctatgt acaccacgtg atgagatgca acggccatcc gatgttaggg caccccaggc
     3181 tgcagggaag gtcgtggtca agttcggagg ctcctccatc ggggggatgg accggatcga
     3241 tgcgttcgcc atgcaggtgg cggagctcaa ggagcgcggc ctggtcccgg tcgtcctgca
     3301 cgggggaggg ccggagatca ctgatgagat gaagcgctgt ggcctcccgg tgaggaaggt
     3361 ggcgggactg cgcatcaccg acgacgcgac gctcgagata gccaagtccg tcctttcccg
     3421 catcaacgac cgggtggtgg tctccctgcg caaggcaggg ctcaggagca tagggctggc
     3481 cggaagcgag gtggacacgc tggtcgcgag gaggatgccc ccggtcctcg tgaacggccc
     3541 cgatgggcgc gaggaggtgg acctcgggta cgtaggggag gtggtgaagg tcgatccccg
     3601 ggcggtgacc gcgctctgcg cgaagggctt cgtccccgtc atctactcca tctgcgccga
     3661 tgcatcgggc atgctgatga atgtgaacgc cgatacggcg gcggccgcgc tcgccgccgg
     3721 catcggggcc tcagacctcg tattggtcac cgaggttccc ggcgtgcttc ggacgatcga
     3781 tgaccttagc tctacggtcc ccgagataag ggaggcggac ctagggccgc tcacctcgtc
     3841 cggggtcctg agggacggca tgatccccaa ggtggaggca tgcttcatcg ccctgcgcgg
     3901 aggggtcggc acggcccaca taatatgcgg cacgacgccg aacgccatag tggaccaatt
     3961 gttcggcgga aggaacctgg gcaccaggtt gacattgtga agtgatccca tggacagcaa
     4021 gcaggttgcg gaactgagct cctactatct attccagaac tacgcccgcg aggctctgtg
     4081 cttctcgcac ggcaaggggg agtacctctg ggacctcgaa ggcaacaggt acatcgacta
     4141 cgtggccggc atcgcggtca actgcctcgg ccatgcccat cccgagctcg taaaggccat
     4201 ctgcgagcag gcctcccgcc tcatccacgt ctccaacctg taccaggtga aggagcaggc
     4261 ggacctgggg gaggccttgg ccgctatcgt ccccgcgccc ctcggccgct cattcttctg
     4321 caacagcggg gcggaggcca acgaggccgc cctgaagctg gcggtgaagc acaccggccg
     4381 ccccaaggtg gtcgccgccc gcaactcctt ccatggaagg accgcggcca ccctctcggt
     4441 caccgggcag gggaagtatc agaagggctt cgagcccctg ctctcccgga acgtcgattt
     4501 catcggctac aactccgtgg aggagctgaa ggccgccgtc gaccatggga ccgccgccct
     4561 catcctggaa ccggtacaag gagagggagg ggtcatcccc gcctcccagg agttcttccg
     4621 cgccgcccgc gatctgtgca cagacaccgg ggcattgatg ctcgtggacg aggtccagac
     4681 cgggatggga cgcaccgggg aatggttcgg cttccagcac tacggggtcg tcccggacgt
     4741 catctccctg gccaagggcc tggggggagg ggtcccgatc ggggccatca tcacctctcc
     4801 ggacatcgcc aaggccttca cccccggcgt ccacggcacc accttcggtg gaaatccgct
     4861 ggcctgcgcg gccgcgagcg ccgtcatccg ggtgctcagg caggagaggc tcgtggaaag
     4921 ggccgcggaa ttgggggcgg agtggaagcg ggagctggtt cggaccgtgc aagggcatga
     4981 gggggtgacc gaggtccggg ggatggggtt gatgctcgga gtggagctgg gcgatcttgc
     5041 caagggattc caggcatatg cattggcacg gaggctgctg gtgaacgtcg cgggcgggag
     5101 gaccgtgcgc ctcgtcccgc cgctgatcat ctccaaaggc tccatccagg agctcgatgc
     5161 cgcactgagt tcgtatctcc acgggaattg acgttccagt catcatcgca cgctcaggga
     5221 ggcgcgttcc atccgtaatc ttcacagggc gaacgtgagc gcttgagtcg ggattggtca
     5281 gcacggccta cggcccaagg ccaagggcgc gcacggaaat gatgtctaaa atagaacatt
     5341 caaatatccc gagcatggta tcgggtcgat gaaagagccg cctcagaagg agagcgtggc
     5401 cgagcgtacc cgtgcctaca tagacgccca tccgtcggtc aaggactgca tcgccaagga
     5461 cctgatcaac tactcctcgc tcgccaggat gataatgaag gacctggata tcaagaacga
     5521 ggaggcggtc atgatcgcct gccgtcggta tgcccagaag ctcgccagga aggaccatga
     5581 aggggagata ctgtccattc tggcggacag ccgcctggag gtcaagacca agatctgcat
     5641 cgtcaccgcc agcaacgact gggctgtgct gcagaggctg gaaaggctct ttgccaagct
     5701 tatcaaccag aaggccctga tgcaggtcat ccagggggcc caggcgatca ccatcatagc
     5761 tgacgagaag ctgcggagcg aggtcgtgac cgaggtcggc gagggcaacg tcctcaaggt
     5821 ccgcgaggac ctggtggaga tcacggtcaa gagcccccag aggatcgggg agaccagcgg
     5881 cgtgttctcg tacctggcgt ccaacctcgc cgagcagggc gtgaacatag tggagacggt
     5941 gtcctgctat accgacacca tcttcatcgt tcacgagaag gacatgatcc cagcctattc
     6001 gctcctctcg aacctgctgg agagttccga gagggtcacc gaggccaggc attagcgttc
     6061 caaagccatt tatgccttgg aaccctatcg aggaccgatg atggaccagg caagggaaag
     6121 ggcgccccgc gtcctcacgg acattccagg gccgcgggcc agagggatag tggagcttga
     6181 tgaccgctac ttggccacag cgacgaagtc cgcccccctg gccgtggagc gggcggaggg
     6241 cgagaaggtc tgggatgtgg acggcaacct gttcctggac ttcagtagtg gcgtggccgt
     6301 catgaacata gggcattgcc atccctcagt ggtcagggcc gtccaggagc aggcggccaa
     6361 ggtgatgcac ttcgcaggga ccgactacta ttacgatgtc caggcccggc tggccgaacg
     6421 gttggccagg atcgcccctg gccgaggcga gaagaaggtg ttcctgagca acagcggagc
     6481 ggagagcatc gaggcggcca tgaagctcgc ccgatggggc tccggacgga ggcagttcct
     6541 ggcattcatg ggggcgttcc acggcaggac cttcggcgcg ctctccctga cctgcagcaa
     6601 gcgcgtgcag cgggccaggt acttccccat ggtgccaggg gtgacgcaca tcccatacgc
     6661 gtactgctac cgctgcccct accatcagga atacccgtcc tgcgggctat ggtgcgccaa
     6721 ggtcctggac gaggtctacc tcgatgccct ggtccccccg gacgaggtgg ccgccctgtt
     6781 cttcgaaccg gtgcagggcg agggcgggta catcgtgccc cctaaggcct ttctgcccga
     6841 gatcgcccga atatgtcgct cgcatgggat cctcctggtg gacgatgagg tccaggccgg
     6901 gatggggcgc acgggcagga tgtgggcggc cgagcatttc ggggtcgagc ccgatatcac
     6961 ctgcatgtcc aaggcgctgg ggtcggggat gcccatagcg gccacggtgt tccgcaagga
     7021 gctggactgg ggggtccccg gcgcgcattc caatacattc ggcgggaact gcgtggcctg
     7081 tgccagctcc cttgccaccc tggacgtcat cgagagggag ggggtcgtcg agcaggccga
     7141 gcgcaagggg gcggtcatgg agaggcgcct cctggagatc aaggacaggt tcgaggccgt
     7201 gggggacgtg cgcggcctgg ggatgatgag ggcactggag ctggtggagg accgccggac
     7261 gaaggccccg gcggccaagc tccgcaacgc catcctcgcc aacatgctca ggaagggcgt
     7321 catcgccctg ggctgtggga ggtccagcat caggctggtc cccccgctca ccattgccga
     7381 ggataacctg gacaccggga tgcgcctgct cgaggagagc atcgcggagg cggtgaggga
     7441 gagggcatga ggctcttccc acgctccggg catcggaagg tgcaaggcga tggctacctg
     7501 aagcttgtca tgctggaggg taaggacata ggggtgagcg ggacgctgct ccaggaggtc
     7561 cagttcaccg ccgggcaaag ggtaaggatg cacttccatc aggccacccg ggaggtgttc
     7621 tactgtctgg agggcccggc gccgttcgtc atcaacggcg agaaggtggt catggaggag
     7681 ggggactgcc tggtctgcga gcccggggac gtgcacggca accccgtcat cgggaacgac
     7741 ttccggatac tggtgctcaa ggtcggcctg agggagcacg acacggtttg gctggactga
     7801 tcccctgtcg cgcagggcgc atcgccccgc accgccgagc cccgtggagg caatggcaga
     7861 atgagggcta ggccctgggc cagcgacccc cgggggtcgg ctgcacagcg tgcgcagagg
     7921 accgggaagg gtaggtaatg gataagatgg aaggcacgcc cgacatggtc cgcacgggat
     7981 cctgctccat ggggggcaga aatgcaagga gccactaggg agatttgaac tcccggcctg
     8041 ctgattacaa gtcagccgct ctaccagctg agctatagtg gcctcgcttc aggaatgccg
     8101 tcctggattt aaccattttt ataggtggct cagcgttcaa tgccggaaac aaggtaatga
     8161 caaaacaacg gttaaatagg cctgcaacat ctgagtgcag ggtgcgccct tggacgccga
     8221 tctcgacagc aaaccggaaa tgagggtggt gatggacagg accgggaacc ccgcaccgct
     8281 gggacttctg gggttcggca tgaccaccgt gatgctcaac ctgcacaacg ccgacctgat
     8341 accgctgagt agtgtcattc tatcgctcgg aatattctac ggaggactgg cgcagctgct
     8401 ggtcgggctg atggagtgga ggaagggaaa caccttcggc atggccgcgt tcacatccta
     8461 cgggctattc tggctgaccc tggtgggcat ctggaccata ccctctctgg gcctgggaga
     8521 gcctaccgac agtactgcca tgggctgttt cctcgccatg tggggcatat acaccctgtt
     8581 cttcttcatc gggacggcaa agagcaaccg ggccacgcag ttcgtgttcg gctcgcttgc
     8641 agtcctcttc ttcgctctcg ccctaggcga tctcaccctg atcaaagaga tcaccgtgac
     8701 cgccggctac atcgggctgg tatgcggtgc ttcagccatc tacactggcc tggcgacggt
     8761 ggtcaacgag gccaacggcc gggccatctt cccactgggg gatcgaaggt aggtcgagaa
     8821 acccgttcac ccctcctttt acgacagcct tttgacaggg agcggcgacg catctttata
     8881 gagcaagctg cgatggacgt cgggctcgcc gtgatcggcg ttcaggtgac atcgatggac
     8941 aaggaatcat ggaaattcct gcaggagctg tgcgatatcc cggggccatc cggcttcgag
     9001 gctgcggcga ccagattgat gaaggactat gccgggaact actgtgatcg ggtcttcacc
     9061 gacaagctcg gcaacaccat gttcgagaag gtgggccaga aggacgggcc gacagtattg
     9121 ctggcaggcc acgtcgatga atgcggcttc atcatcactg gcgtgaaccc gcaaggatac
     9181 ctggcgttca gccaattggg cggatggttc gaccaggtat tgttaggtca gcgggtcctg
     9241 gtccacacca ggaaaggcgt tctgaacgga gtgatcgcct gcaagccccc tcatctgatg
     9301 gagccagagg aggccaagaa ggtggtcacc aaggacaaga tgttcattga tgtgggcgcg
     9361 tgcaacaagg aagaggccca ggccatggga gttcgcctgg gggacgcggt caccccggat
     9421 tccaggatgt tcactgtgga gaagaaggcc tacaaggacg ggaaacctat cggcaagcgc
     9481 acgatcgtct ttggaagagc tttggacaac aggctgggag cgttcgcggc ggtggagctt
     9541 ctcaaagcga taaagaaggg aggagtgaag caccccaaca ggctggtggc cgcagcgacc
     9601 gtccaggagg aggtcggtct acggggcgcc aaaacggtgg cggcctccac cgatcccgat
     9661 gtggccatcg ttctggacgt ggacatagcc ggggacgtcc caggcatcga cccgctgcag
     9721 gcccctgcgc ggatgggcga gggcgtttca attactgtct acgatggaac aatgatcccg
     9781 aaccagccgc tcaaggagct ggtcatctca atttgcgaga gaaagaaaat accatatcag
     9841 ctggcctacg tcatccgcgg gggcaccgac ggaggttcaa tccacacctc cctgaccggc
     9901 gtcccgaccg tcgtcatcgg cgttcccacg agacacatcc actcacatgt gggattgttc
     9961 gacaccgctg acctgaaggc gtgcatgaag ctcatcgtgg aactggccaa ggcgcttgac
    10021 cggaggacgg tggactcctt caccgccctc tgagcaagcc gttccggagg cggtccacac
    10081 cgtactgtgg ggattgcctg gaacacctca ggcggcgcta tggggagagt gtccgtacat
    10141 cggctggacg ttctgcatct tccccaggca tgggaacgat gcgatggggc tcgtgagcga
    10201 tattccctcg ctccacccca gctcgctctc tggacatcgc cccccatccc ctcgctcgac
    10261 gaaccaccgg accaatctcc acaaccgact acggacccca ccgcaggtca ctggtctcag
    10321 tccgctcgcc ggacgcccct cggcgatgcc ccagcctccg gtccgtggtc tcctcggatc
    10381 agtggggcgc caggaggcca agccacagcc gataggttcg atgcgcccac tcctcctggc
    10441 gctagcggtc gcgcccatcg ctctcctaca agtggccccc ggcccacctc tcttcagctt
    10501 ggacgcccat cgccggagcg gccatgcgtc catctccgca tcgcaggaca gatgaatggg
    10561 aaggtccatg ccgcggaggc gatctccccg accgcgcccg atcttacagt gccccagccc
    10621 ttgaggtaca ggggattacg gaagggccga tccccctcac cagacagtga tcgcagcgcc
    10681 tcatgggatt ccgggcgcga ccggcctctc aacatcgagc atatgcccta acgatacccg
    10741 caccgttcct ctcggaaaaa tgtcgttgcc caccacacct tgagaatgtg cacatactcc
    10801 tcccttggcc gagacaacgt tcctttttcc gtgcccacct gaacgtccga aagatataaa
    10861 aagagggaat caatcaacca gtccgccaaa tcaggatcac tggtgaattt catgaccgat
    10921 ggaccaacga ccgcaaaggt ctccaagggc gacatcgtcc acgtggacta tgacgcgtac
    10981 atcgccgaga gcggcatgct gttcgataca accaacgcgg acaaggccaa ggaggccggc
    11041 atcttcaatg agaacctgac ctacaagccc atgccggtgt tggtgggcgg cggaagggtc
    11101 ttccccggcc tggacgaggc cttggccgag gccaaggtcg gagagaggac gaccgtggag
    11161 atccccccgg agaaggccgc cggggccagg gaccccaagc tggtggagaa catccccctg
    11221 cgtgaattct tccgccagga catccgcccg gaggtcggca tggaggtcaa tatcaaggac
    11281 cgcgtgggca cggtcgtcgc agtgaccaac aggatggtcc gcgtggactt caaccgccgc
    11341 ttcgccggcg ccgcgctcag gtatgagttc actgtactgg acaaggtaga ggacgccgtg
    11401 gccaaggccc tcgccatctg cgagatggac tacgagacct ccgagggctt ccgagccgtc
    11461 gtctccccgg acaaggtgac cctgtacctt ccggacgtat gtaagtacga cccgaagtgg
    11521 ctgctctcca agtacaaggt cgtggccgat ctgcgcgagg tcttcggccg ggtcgacgtc
    11581 gaactggtgg aggaatacct caagaaggag gagaagccca ccgaggagaa gcctaccgag
    11641 gcacaggcca aggccgccga ggagaaggcc ccc
//
LOCUS       DAOSYN010000022        11531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_4766_length_11531_cov_4.261764, whole genome shotgun sequence.
ACCESSION   DAOSYN010000022 DAOSYN010000000
VERSION     DAOSYN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 11531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11531
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4766_length_11531_cov_4.261764"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..905)
                     /locus_tag="PLJ11_01895"
     CDS             complement(204..905)
                     /locus_tag="PLJ11_01895"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR03679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOO03454.1"
                     /translation="MRLASLFSGGKDSTYAAYLAEQAGHEVPCLVSVVPRDPHSWVFH
                     TINLEHLPDMAKAMGRTLVAVPSSGEEEGDLAALEQALSALDVDGVVTGAIASDYQWD
                     RINGVCDRLGLRTLSPLWRKDQLLVLEDMLQAGVKAMVVGVFSGGLGEEWLGRMLDRG
                     AVSALAALASAKGMNVSGEGGEYETLVLDSPMHHWELVPEGVRREYHRDSGRLFIERL
                     GACEHMRLGEGSRRS"
     gene            904..1173
                     /locus_tag="PLJ11_01900"
     CDS             904..1173
                     /locus_tag="PLJ11_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03455.1"
                     /translation="MDVNVRANITNVGQTEVPHGQALKLRLLDTLSGAKMPLHCPRCR
                     KDIDKAKVEEIDSKLMGMFQNDSLRRGVCPFCQTPLIDTDRKVKR"
     gene            1170..1877
                     /locus_tag="PLJ11_01905"
     CDS             1170..1877
                     /locus_tag="PLJ11_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOO03456.1"
                     /translation="MRMAEMSSPAFAEAVKGDPIVFLPIGAVEAHGPHLPLGTDTFQP
                     EFVVNAVCERVGGLIAPTINYGQHSSTRNLPGTIDISFDTLRALVMDILDALRRNGVR
                     KVVVISGHFGSVHRAAVKLACERAARQGMRVMFLSDYELAELRAQVVSEGLPDGHGGA
                     LETSRMMDIRPDLVAEARTAGEFVSMDFMVRADPERCLPQGFGGDARMATPEKGRLVN
                     QFVVERLTELVRRNFEG"
     gene            1879..2553
                     /gene="rpiA"
                     /locus_tag="PLJ11_01910"
     CDS             1879..2553
                     /gene="rpiA"
                     /locus_tag="PLJ11_01910"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOO03457.1"
                     /translation="MSDRKRLAAEKAVELVRDGMKVGLGTGSTAYYAIEAIGRLVQRG
                     YALQAVPTSLASERQARELGIPLTTLDEVGMLDITIDGADEVDGNLDLIKGLGGALLR
                     EKIVAHSTRTEVIVVDDSKLVRTLGTRSPLPVEVVPFSHGRTRLYMEELGCQAELRGG
                     DRPFVTDNGNYIYHLHFERGIRDPYGLQQRLREIPGVVETGLFLKMAKKVFVASDEGV
                     RVMERV"
     gene            complement(2554..2925)
                     /locus_tag="PLJ11_01915"
     CDS             complement(2554..2925)
                     /locus_tag="PLJ11_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOO03458.1"
                     /translation="MADKGFNKKDESLVDLLVNTGMPKNVAKTLAFMRKKEETTSVEI
                     EIMTALRQPEVSIAMQELRRRKWVIKRDIKKEGKGRPVHAYKLAIPFEKIIETIEKEE
                     RKRIESIERNIEQLKALSQSP"
     gene            3380..3652
                     /locus_tag="PLJ11_01920"
     CDS             3380..3652
                     /locus_tag="PLJ11_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HOO03459.1"
                     /translation="MPRSIKTYCPSCKTHTEHEVERVKKKKASEMKWGQRRFRRATAG
                     YGGFPRPKPEGREKPTKRIALRYRCKKCKKAHQKACFRAKKFELTE"
     gene            3658..3831
                     /locus_tag="PLJ11_01925"
     CDS             3658..3831
                     /locus_tag="PLJ11_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HOO03460.1"
                     /translation="MPTTGKFIKVKCQDCGNEQITYKKPAMNVTCAVCGSTLVRSRGG
                     EGEIKGELLEVVD"
     gene            3831..4613
                     /locus_tag="PLJ11_01930"
     CDS             3831..4613
                     /locus_tag="PLJ11_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HOO03461.1"
                     /translation="MARVNEFPDEGELVVCTVQSVKNFGAFVTLDEYGGKEGFIHVRD
                     VATGWVKYIRDYIREGQKVVCKVLGVDAAKGHIDLSLKSVNEHQRREKVQQWKNETKA
                     EKLLEIVANRLGRTPDQCWEEFGYELVDRFGTLYTAFESCAMDEDALKSAGIAGEWTP
                     VFIEVAKENFPPPFVQIAGTLELRSPAPDGVERLKAALKKGLDASKEELHIQYIGAPR
                     YRLVMTAPDYKTAEEAMKEVTGLIISEIKESGGDGAFHRDSK"
     gene            4674..4835
                     /locus_tag="PLJ11_01935"
     CDS             4674..4835
                     /locus_tag="PLJ11_01935"
                     /inference="COORDINATES: protein motif:HMM:NF009623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HOO03462.1"
                     /translation="MKTTLRLCEGCAEYTLKDTCPRCGVKTSLPIPPRYSPEDRYGEY
                     RRRARQETR"
     gene            4841..5599
                     /locus_tag="PLJ11_01940"
     CDS             4841..5599
                     /locus_tag="PLJ11_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HOO03463.1"
                     /translation="MDSVIMVMRERPDLRAPILIEGLPGVGNVGKLAAEHLVEQMKAV
                     KFADIYSKHFPPQVLLDDDGVIRLVDNEMHYSKGEGKRPDIIILTGDYQGMTPDGQYE
                     LSYHAIQVCKELGVSRIYTLGGYGIGKMVESPRVLGAVTDISLCAEMAAHGVVFSKGE
                     PGSGIVGASGLMLGLGKMEGIPAVCLMGETSGYFVDPKGAEAVLRVLMALLHLDIDLS
                     ALTDKAEQIDLITNKLKEAEAPEAPRREDLGYIG"
     gene            complement(5988..6227)
                     /locus_tag="PLJ11_01945"
     CDS             complement(5988..6227)
                     /locus_tag="PLJ11_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOO03464.1"
                     /translation="MEEGDLEKVTLRLPTRHIRALDFLVQVDDFPSRSEAIRAAIRDF
                     IYARVDLVTDKMKKMEEAERVLAEMEAYEERYLRK"
     gene            6416..6527
                     /locus_tag="PLJ11_01950"
     tRNA            join(6416..6454,6489..6527)
                     /locus_tag="PLJ11_01950"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6451..6453,aa:Tyr,seq:gta)
     gene            complement(6640..7938)
                     /locus_tag="PLJ11_01955"
     CDS             complement(6640..7938)
                     /locus_tag="PLJ11_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO03465.1"
                     /translation="MARSVLIRNGMIITQDRDRRVLQGDVLVEGGLIAEVGKVRGGAD
                     VEIDAKGAPILPGLINAHCHVAMTVMKGMVDDLPFGEFLDRTFSIDSDRQDDDLLAGS
                     RLGCLEMLMGGTTTFVDLYYSQDVIARAVKEAGMRGVLCWAVLDQQFTTQRGVPLENC
                     KAFHSRYAGDEHILPGIGLQGVYVCSEETFMGARAFSDRTGALMTFHLSETRKEVYEH
                     KAKTGKRPADWLASIGFLNERCLAAHSAWLTIGEVRALAKAGASIATCPVSNMKLATG
                     GVAPIPEMLREGVKVTIGTDGSTTNNSLDMFGEMKALALLQKASRWDPTVVPAQEALD
                     FATVSAAAALGLEREIGSIEVGKRADIVIMDHASPAIRPLTKENAVSNMVYSAGRGDV
                     RSVLCYGNVVIKDRVPQTLDLGQVIEDAQGAMAKIAARVG"
     gene            8057..8857
                     /locus_tag="PLJ11_01960"
     CDS             8057..8857
                     /locus_tag="PLJ11_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO03466.1"
                     /translation="MTRLLFLGTGGGRFSTIYQIRATGGLYVMDGANLHIDPGPTAAH
                     QMHRAGLDPAKTDAVLVSHCHPDHYTDAEVMIEGMSRGGLKKRGALLASRSVLEGQQL
                     HGPAISNYHRSLLERIEMAEPGGSAEVDGMRIRFTSTRHSDPTGVGFSMETRDGIVSY
                     VSDTELDERVQREQEGSRVLILPLTRPWGARIPNHLCTEDALEFVKAVRPEMAILTHF
                     GAKIIHAGADRQAAMIEKGTGVRTVAAEDLMSVQLGRTIRLHRFSKGF"
     gene            9009..10334
                     /gene="cca"
                     /locus_tag="PLJ11_01965"
     CDS             9009..10334
                     /gene="cca"
                     /locus_tag="PLJ11_01965"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR03671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOO03467.1"
                     /translation="MPLEEDVLRRLRPSPRTLRELDEAVGRLLNATEERISGTGLDLR
                     TMLVGSVAKGTFVGRPDIDLFVLFPTSVERGELERIGLQIGREVLGGETRYAEHPYTH
                     GRLSGFDVDLVPCYAIEDPTRLRTAVDRTPFHTKYVLEHLTDELREQVLLLKQFMKGV
                     GVYGAEAKVRGFSGYLTELLVISYGSMQKVLRAGAGWRPGIVMDMDGRRKGKGSSPLT
                     FWDPVDPKRNVASALSLDRFSLFVHACREYLSAPSERFFFPRKRQPLGTAEARMAFEG
                     RGTTPFVVVLDRPELVDDNLYPQVERSLGGLRRLLEMYGFQVQDAAYSVGVDVRMAFE
                     LSSALLPPTRLHQGPPAWTENAEEFVRRWREEGVGQPFLAEGRWMVYAKREFRDPASL
                     IMARGAEAALGNSFKGLPGLRCHAGEKAFLAANRQLFTGLLDRRESWRV"
     gene            complement(10331..10999)
                     /locus_tag="PLJ11_01970"
     CDS             complement(10331..10999)
                     /locus_tag="PLJ11_01970"
                     /inference="COORDINATES: protein motif:HMM:NF039857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03468.1"
                     /translation="MAHKKRKEPAVKEEYRFVPPDFNEKEFLEKDIAITKTVLLSALL
                     AVVFGVIAYFTTDITFIIGLLLILVGAVALRKMIELFPLDLSAVENKTWLGNGLMFFF
                     LALGVWILLLNPPFGDVIEPQIRDLEVWVGDVEYQRPYQNVPVGNVTFNVTVFDNGEL
                     SKVQFSFIGSEGSWDMVKGEDGRYEYTHTFEVPGTYTFEITAVDKAGNTNTYRSSIGI
                     IEPG"
     gene            11173..>11531
                     /locus_tag="PLJ11_01975"
     CDS             11173..>11531
                     /locus_tag="PLJ11_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO03469.1"
                     /translation="MANANIEELPGVGPATADKLREAGYTNLMTIAVESPKTLADACD
                     IGEATAIKIIDAAKKAAEVGGFESGDVILERRRNLSRLTTSSKAFDELLGNGLESQSI
                     VEFFGEFGSGKTQVCFQ"
BASE COUNT         2255 a         3364 c         3692 g         2220 t
ORIGIN      
        1 cggggggcga tgccccggag gcgtccgagg ggagaccgtc accgccatag gcggccgggc
       61 gggggcggcc gttccccgat tcgaccgatg aggccgccat ggatgttatc cgttcccggc
      121 gacgaaacga tgcggccgtt cgatcggcgg cgcaagatct agggccgatc ggagctttcc
      181 ctggggccgg agcgtgcccc cctctatgat ctacgggaac cctcgcccaa ccgcatatgt
      241 tcgcaggcgc ccagcctttc gatgaacagc cgtcccgagt cgcgatggta ctccctccgc
      301 accccctcgg ggaccagctc ccaatggtgc atcggggagt ccaggaccag ggtctcgtac
      361 tccccgccct ccccggagac gttcatcccc ttggcggagg cgagggcggc cagggcggag
      421 acggcgccac ggtccagcat ccttcccagc cattcctccc ctagaccgcc ggagaagacg
      481 cccacgacca tggccttgac gcccgcctgc agcatgtcct ccaggacgag cagctggtcc
      541 ttgcgccaga gaggggagag cgttctaagg ccgagccggt cgcacacccc gttgatgcgg
      601 tcccactggt agtcggaggc gatggccccg gtgacgaccc cgtcgacgtc cagggctgac
      661 agtgcctgct ccagcgcggc caggtcgccc tcctcctcgc cggaggaggg cacggccaca
      721 agcgtcctgc ccatcgcctt cgccatgtcg ggcaggtgct ccaggttgat tgtgtgaaag
      781 acccaggagt gggggtcccg cggaacgacg ctcactaggc acggcacctc gtgcccggcc
      841 tgctccgcaa ggtaggcggc ataggtggag tccttgccgc ctgagaacag ggaggccaag
      901 cgcatggatg tgaatgtccg ggcaaatata accaatgttg gccaaaccga ggtcccccat
      961 gggcaagcat tgaaattgcg cttgctcgat accctttccg gtgcgaagat gccgctccat
     1021 tgcccccgat gcaggaagga cattgacaag gccaaggtgg aggagatcga ttccaagctc
     1081 atggggatgt tccagaacga ctccctcagg cggggggtct gcccgttctg ccagacgccg
     1141 ttgatcgaca cggacaggaa ggtgaagaga tgaggatggc cgagatgagc tccccggcct
     1201 tcgcggaagc ggtgaagggg gaccctatcg tcttcctgcc tataggggcg gtcgaggccc
     1261 atgggccgca cctccccctg gggacggaca ccttccagcc cgagttcgta gtgaacgcgg
     1321 tgtgcgagag ggtgggcggg ctcattgccc cgacgatcaa ctacggacag cattcctcca
     1381 cgcgcaatct tccggggacg atcgacatct cgttcgatac gttgcgcgcc ctggtcatgg
     1441 acatattgga cgcgctgcgg cgcaacggcg tccggaaggt cgttgtgatc tcaggccatt
     1501 tcggttcggt gcatcgcgcg gcggtgaagc tggcctgcga gcgggctgcg cgccagggaa
     1561 tgagggtcat gttcctcagc gactacgagc tcgccgagct cagggcgcag gtcgtgagcg
     1621 aggggctccc ggacggccat ggaggggcct tggagacctc caggatgatg gacatcaggc
     1681 cggacctcgt tgccgaggcg cgcaccgccg gggagttcgt gagcatggac ttcatggtcc
     1741 gggcggaccc ggagaggtgc cttccccagg ggttcggagg ggacgcccgc atggcaaccc
     1801 cggagaaggg ccgcctggtg aaccagttcg tggtggagag gctgaccgag ctcgtgcgcc
     1861 ggaacttcga gggataggat gagcgatagg aagcgcctcg ccgcggagaa ggccgtggag
     1921 cttgtgcggg acgggatgaa ggtcggcctg gggacgggga gcacggccta ctacgccata
     1981 gaggccatcg gccggctggt ccagagggga tatgcgcttc aggccgtgcc cacctcgctg
     2041 gcgagcgagc ggcaggcgag ggaactgggc atccccctga ccaccctgga cgaggtcggg
     2101 atgttggata tcaccatcga tggggcggac gaggtggatg gcaacctgga cctgatcaag
     2161 ggcctggggg gggcgctgct cagggagaag atcgtggcgc attccacccg gaccgaggtg
     2221 atcgtggtgg acgattccaa gctggtgcgg acgctcggca ccaggtcccc ccttccggtc
     2281 gaggtcgtgc cgttctcgca tgggcgcacc cgattataca tggaggagct gggatgccag
     2341 gcggagctgc gcggcggcga ccggccgttc gtcaccgaca acggcaacta catctaccat
     2401 ctgcacttcg agcgggggat aagggaccca tacgggctcc agcagaggct gagggagatc
     2461 ccgggggtgg tggagaccgg gctcttcctc aagatggcca agaaggtgtt cgttgcctcc
     2521 gacgagggcg taagggtgat ggaaagggtt tgatcagggg ctttgggaga gcgccttgag
     2581 ctgctcgatg ttcctctcga tcgactctat ccgcttccgt tcctccttct cgatcgtctc
     2641 gattatcttt tcgaagggga tggctaattt gtaggcgtgc actggcctgc ccttcccctc
     2701 cttctttata tcccttttga tgacccattt gcggcggcga agctcctgca tggctatgga
     2761 gacctcgggc tgtctgaggg cggtcatgat ctcgatctcg accgatgtcg tttcctcctt
     2821 cttgcgcatg aaggcgaggg tcttggccac gttcttgggc atcccggtgt tgaccagtag
     2881 atcgaccaat gactcgtcct tcttgttgaa gcctttgtcg gccatgtaat atcaccgttc
     2941 tctgctatta taaatgaata tcaattatta tttgattgat atatatcact ttctatttct
     3001 tgttatttcc tatatctggc caatattctg gcacggtata ttttagtcct gtggaactaa
     3061 aatgagcgtc tcagaatatt tgatgaagat ctcgagggcg cggagcggtg gtacaagctt
     3121 gggatggttc ttctatgacc ggggcatgct gccctgccta gatcccgaag gggccttggg
     3181 caatgcctgg gggtttatac cgatgaacga acgcgatcga agggggcgtt gtttcctccc
     3241 ggctccgccc ccttcattcg agggaacgct cgcatttgac cacccagctt ccttcgggcg
     3301 gggttctttc cctgctaagg gtttaaatag ggaattggta ttagggcact tttgctccaa
     3361 ggggatgaat ccattgaaga tgcccaggtc cataaagact tattgtccat cctgcaagac
     3421 ccacaccgaa cacgaggtcg aacgggtcaa gaagaagaag gctagcgaga tgaagtgggg
     3481 ccagaggaga ttccgccggg ccacggcagg gtacggtggt ttcccccggc ccaagcccga
     3541 ggggcgcgag aagcccacca agcggatcgc cctgaggtac cgctgcaaga agtgcaagaa
     3601 ggcccatcag aaggcgtgct tccgggcgaa gaagttcgag cttacggagt gagagcgatg
     3661 cccaccaccg gtaagttcat caaagtgaag tgccaggatt gcgggaacga gcagatcacc
     3721 tacaagaagc ctgccatgaa cgtgacatgt gcggtgtgcg gctcgacctt ggtcaggtcc
     3781 cgggggggag agggggagat caagggagag ctgcttgagg tggtcgacta atggcccggg
     3841 tcaacgagtt ccccgatgag ggggagctcg tggtctgcac agtgcagagc gtcaagaact
     3901 tcggggcgtt cgttactctg gacgagtacg gcggcaagga aggcttcatt cacgtacggg
     3961 acgtggccac gggctgggtc aagtacattc gggattacat tcgcgagggg cagaaggtgg
     4021 tctgcaaggt cctgggcgtg gacgcggcga aggggcacat cgacctgtcc ttgaagtccg
     4081 tcaatgagca ccagcgccgg gagaaggtgc agcagtggaa gaacgagacc aaggccgaga
     4141 agcttctgga gatcgtcgcc aaccgcctcg gcaggacgcc cgaccagtgc tgggaggagt
     4201 tcggctatga gcttgtcgat aggttcggca cgctgtacac ggccttcgag agctgcgcaa
     4261 tggatgagga cgccttgaag agcgccggga tcgccgggga atggaccccc gtgttcatag
     4321 aggtggccaa ggagaatttc cccccgccct tcgttcagat agctgggacc ctggagctgc
     4381 gctcccccgc cccggatggc gtggagaggc tcaaggctgc tctgaagaag ggtttggacg
     4441 cgtccaagga ggaactccac atccaataca tcggcgcccc ccggtaccgc ctggtcatga
     4501 ccgccccgga ctacaagacc gcagaggagg ccatgaagga agtgaccggc cttatcatct
     4561 cggagatcaa ggagtccgga ggggatgggg ccttccatcg ggattccaag tgagcccggc
     4621 caatactgat ttatcgaggt ccctacctat agggctacgc aggtgaaagg accgtgaaga
     4681 ccactctgag gctgtgcgaa ggctgcgctg agtacacgct caaggacact tgtcccagat
     4741 gcggtgttaa gacctcgctg ccgatccctc cgcgctattc gccggaggac aggtatggcg
     4801 agtaccgccg gcgggcacgg caggaaacga ggtgatccga atggatagcg tcataatggt
     4861 catgcgagag aggcccgacc ttcgggcccc gatattgata gaggggttgc cgggggttgg
     4921 gaacgtaggg aagttggcgg cggagcactt ggtcgagcag atgaaagcgg tgaagttcgc
     4981 ggacatctat tcgaagcact tcccgcccca ggtattgctg gacgatgacg gggtcatccg
     5041 cctggtggac aatgagatgc actattccaa gggggagggc aagcgcccgg acatcatcat
     5101 cctgaccggg gactatcagg gcatgacccc cgacggccag tatgagctgt cctaccatgc
     5161 catccaggtc tgcaaggagc tgggcgtgag ccggatctat acgcttggcg gctatggcat
     5221 cggaaagatg gtggagagcc cgcgggtgct gggcgcggtc acggacatat ccctgtgcgc
     5281 cgagatggcg gcccatgggg tggtgttctc caagggtgaa ccggggagcg ggatcgtcgg
     5341 ggcctccggg ctcatgctcg ggctgggcaa gatggaaggg atccccgctg tctgcctcat
     5401 gggcgagacc tccggctact tcgtcgaccc gaagggggcc gaggcggtgc tgagggtgct
     5461 gatggcgctc ctgcacctgg acatcgatct cagcgccctt acggacaagg ccgagcagat
     5521 cgacctcatc acgaacaagt tgaaggaggc tgaggcgccg gaggcaccga ggcgcgagga
     5581 cctgggttac atcgggtaga ctcttccgtt gttttcgtgt atagaaggca ttgccgcgag
     5641 atcacagtga ccgggctggc ccggtcactg ccagctgatg caagtcgaac atgatgggct
     5701 tgcatcgcac ctcgttctcc ctagcatcac ttttccggca tcggatacga tgcccgaaca
     5761 gatcgccgat cctgttgaac accgttccag catgatcgcg cttcgccgtc ggggcggaga
     5821 agcgctttgg gcatcgcaaa gcgaagaagg ccggcctgct gtgatatttg acaatgacac
     5881 actgtctggt tgaatgctac gacaaggcct ataaaaaaga aaaagagggg aggttgctgg
     5941 ggggaacctc gggcctgact aggtgcatcc catcccttcc tggcaggcta tttcctcaaa
     6001 tacctctctt cgtatgcctc catctccgca aggaccctct cggcctcctc catctttttc
     6061 atcttatcgg tgaccaggtc gacccttgcg taaatgaagt ccctgatggc agctcttatg
     6121 gcctcggacc gggagggaaa gtcatctacc tgaaccagga aatccagagc ccggatgtgc
     6181 ctagtaggca atcgcaacgt gaccttctcc aggtcgcctt cttccatcgt gcgtcctgac
     6241 aacgtgggtc gtttagacct ctattgtccg accacaatat gacctgcgtt ctatttaatt
     6301 gttgtttctc cgagggggac gcagctgctg cgcccagtgg atcgtagagg gcagttccgg
     6361 cctcctttct ctacgataat tgataaatac ggtctccgta ttatggtggc gtacacccga
     6421 cttagctcag tctggctaga gcggctgact gtagtgggac cccggcttgc cggtcatcag
     6481 ccgccgatat cagcaggtgc ccggttcaaa tccgggagtc gggaccattc attttccttg
     6541 catagagccc gtacgcggag aggagggcag gaccgtcgag cgtgcatagg gatgcgatcg
     6601 ggcctggggc atcggccgtg accaaggact tggtgcggcc tatccgaccc tcgccgcgat
     6661 cttggccatc gccccttgcg cgtcctcgat gacctgtcct aggtcgaggg tctgagggac
     6721 gcgatccttg atgacgacat tcccgtagca gagcacgctc cgcacgtccc cccgccccgc
     6781 ggagtacacc atattagaga cggcgttctc cttggtcaga ggacggatgg ccggggaggc
     6841 gtggtccatg atgacgatgt ccgcccgctt ccccacctcg atggaaccta tctccctttc
     6901 cagccccaag gcggccgcgg cgctcaccgt cgcaaagtcc agggcctcct gggcgggcac
     6961 caccgtgggg tcccacctgc ttgccttctg cagtagggcc agggccttca tctccccgaa
     7021 catgtccagg gaattgttgg tggtggagcc gtccgtgccg atcgtcacct tgaccccttc
     7081 gcgcagcatc tcggggatgg gggcgacgcc gccggtggcg agcttcatat tcgagacggg
     7141 gcaggtggct atggatgctc ccgccttcgc caacgccctt acctcgccga tggtgagcca
     7201 ggccgagtgc gccgccaggc accgctcgtt caggaagcct atggaggcca gccaatccgc
     7261 cgggcgcttt ccggtcttgg ccttgtgctc gtacacctcc ttcctcgttt cggagaggtg
     7321 gaaggtcatc agggcaccgg tcctgtccga gaacgctcgg gcgcccatga acgtctcctc
     7381 cgaacagacg tagaccccct gcaggccgat gcccgggagg atatgctcgt ccccggcgta
     7441 gcgggaatgg aaggccttgc agttctccag tggaacgccc ctttgggtcg tgaactgctg
     7501 gtccagcacc gcccagcaca ggaccccgcg catccccgcc tccttgaccg ccctggcgat
     7561 gacatcctgc gagtagtaga ggtccacgaa ggtcgtggtc cctcccatca gcatctccag
     7621 gcagcctagc cttgaccctg ccaggaggtc atcgtcctgc cgatcggagt cgatggagaa
     7681 ggtgcggtcc aagaactccc cgaagggcag gtcgtcgacc atccccttca tcaccgtcat
     7741 ggcgacatgg cagtgcgcgt ttatcagccc gggcaggatg ggcgccccct tggcatcgat
     7801 ctccacgtcc gcaccgcccc tgaccttccc aacctcggct atcagccccc cttcgacgag
     7861 gacgtccccc tgcagcaccc ttcggtcgcg gtcctgggtt atgatcattc cgttcctgat
     7921 cagaacgctt ctagccattg ggcgaggaaa gccctccgtt gataataatc ctatgccgcg
     7981 atcccgttgt catgtgtagc aagtttaatt ttccagttcc tgcatgggac ttcacatcgt
     8041 tacgtaaggg gtcctaatga cgcggttgct gttcctaggc actggaggtg gtcggttctc
     8101 caccatctac cagataaggg cgactggagg cctctacgtc atggacgggg cgaacctgca
     8161 catcgatccc ggcccgaccg ccgcccatca gatgcaccgc gcagggctgg acccggccaa
     8221 gacggacgcg gtcctggtgt cgcattgcca tcccgaccat tacactgatg cggaggtgat
     8281 gatcgagggc atgtcccggg gcgggctcaa gaagagagga gcgctcctgg caagcaggtc
     8341 ggtgctggag gggcagcagc tccatgggcc tgccatatcc aattaccatc gttccctgct
     8401 agaacgcatc gagatggccg agcccggggg gagcgcggag gtggacggga tgcgcatcag
     8461 gttcacgagc accaggcata gcgatccgac cggcgtgggg ttctccatgg agacccggga
     8521 cgggatagtc tcgtacgtca gcgatactga gctggatgag cgcgtgcaga gggagcagga
     8581 ggggagcagg gtgctgatcc ttccgctgac ccgcccgtgg ggggcccgga tcccgaacca
     8641 tctatgcacc gaggacgccc tggagtttgt caaggcggtg aggccggaga tggccatctt
     8701 gacccacttc ggcgctaaga tcatccatgc gggagcggac aggcaggcag cgatgataga
     8761 gaaggggacc ggcgtacgca cggtggccgc agaggacctc atgagcgtgc agttaggcag
     8821 gacgatacgg ctgcatcggt tcagcaaagg gttttaaaaa ataatatata gtagagcata
     8881 catagtacaa aacgcagcgc aaaaaggtcg gtggctcttc ctcctattgg ttctgaactc
     8941 ccccaccaca ccgacccgct gcgatttttc cctaaaagat gataatgccg tcccccaatg
     9001 cccaccccat gcccctggag gaggatgtac tgcggaggct cagaccctcc cctcgtacgt
     9061 tgagggagct ggatgaggcg gtaggtcggt tgctgaacgc taccgaggaa cgcatctccg
     9121 ggaccggcct ggacctgagg accatgcttg tgggttcggt ggccaagggc acgttcgtcg
     9181 gtaggcccga catagacctg ttcgtgctct tcccgacatc ggtggaacgc ggggagctcg
     9241 agcgcatagg gttgcagatc ggccgcgagg ttcttggcgg ggagacccgg tacgccgagc
     9301 acccctacac ccacggccgc ctctcgggct ttgatgtgga cctcgttccc tgctacgcga
     9361 tcgaggaccc caccaggctc cggacggcag tggaccgcac ccccttccat accaagtatg
     9421 tccttgagca tctgacggac gagctgcggg agcaggtcct cctgctcaag cagttcatga
     9481 agggggtggg ggtgtacggg gcggaggcca aggtacgggg gttctccggc tacctcacgg
     9541 agctgctcgt gatcagctat gggagcatgc agaaggtgct cagggcgggc gctgggtggc
     9601 gccctggcat cgtaatggac atggatggtc ggaggaaagg caagggcagc tcccctctga
     9661 cgttctggga cccggtggac cccaagagga acgtcgcctc tgccctttcc cttgaccggt
     9721 tctccctgtt cgtccacgcc tgccgggaat acctgagcgc tccgagcgag cgcttcttct
     9781 tcccccggaa gcgccaaccc ctgggaacgg ccgaggcgag gatggccttc gaaggccggg
     9841 gcaccactcc gttcgtcgtc gtcctagacc gaccggagct ggtggacgac aacctgtacc
     9901 ctcaggtgga gcgaagcctt ggcgggctcc gaagactgct ggagatgtat gggttccagg
     9961 tgcaggacgc tgcgtactcg gtaggcgtgg atgtgcgaat ggccttcgaa ctctcgagcg
    10021 ccctactccc cccgacgagg ctgcatcagg ggcctccggc ctggaccgag aacgcggaag
    10081 agttcgtacg caggtggaga gaggagggcg tcggccaacc gttccttgcc gagggaaggt
    10141 ggatggtgta tgccaagcgg gagttcaggg accccgcctc tctgataatg gccagggggg
    10201 ccgaggccgc tttggggaac tcattcaaag gacttcccgg gctgagatgc cacgcggggg
    10261 agaaggcctt cctcgcggcc aaccgtcagc tgttcaccgg gctcttggac agaagggaga
    10321 gctggagggt ttaaccgggt tcgatgatgc ctatcgatga gcgataggta ttggtgttcc
    10381 ccgccttgtc caccgcggtg atctcgaacg tgtacgtccc cgggacctcg aaggtatggg
    10441 tgtactcata gcgcccatcc tcgcccttga ccatgtccca tgacccctcg gaccctatga
    10501 aactgaactg gacctttgac agctcgccgt tgtcgaagac cgtgacgttg aacgtgacgt
    10561 tcccgacggg cacgttctgg tatggccttt gatactccac atcccccacc caaacctcca
    10621 aatcacgaat ctgtggttca atgacatccc cgaaaggcgg gttgaggagc aggatccata
    10681 cgccgagagc gaggaagaag aacatgaggc cgttccctaa ccaggtcttg ttctccaccg
    10741 cgctgaggtc taacgggaaa agctcaatca tcttccgcag ggccaccgcg ccgacgagga
    10801 tcagtagaag gccaataatg aacgttatgt cggtggtgaa gtatgcgatg accccgaaga
    10861 ccaccgccag gagagcgctg agcaggacgg tcttggtgat ggcgatgtcc ttctccaaga
    10921 actccttctc gttgaagtcc ggaggaacga acctatactc ctccttgacc gctggctcct
    10981 ttcgtttctt atgggccatg cccctcctta ataataactc ggtaataaac ccttttgatg
    11041 aggcgaacgc ccattgtaat cgccaatgat taatggggaa atgcctatgc tcttctacgg
    11101 tcggaacact ttcgaccaac ccccaaacac ctttttaata tgcccgatgg atagggaagg
    11161 ataggtgcag acatggcgaa cgccaacatt gaagagttgc ctggagtagg acccgcaact
    11221 gccgataagc tcagggaagc tggctataca aacctcatga ccatagcggt ggagtccccc
    11281 aagaccttgg ccgatgcatg cgacatcgga gaggccaccg ccatcaagat catcgacgcc
    11341 gccaagaagg ccgccgaggt gggcggcttc gagtccggcg atgtcatatt ggaacggagg
    11401 aggaacctca gccgcctgac cacaagttcc aaggccttcg acgagctcct gggcaacggg
    11461 ctggagtccc agtccatagt ggagttcttc ggggagttcg gaagcggcaa gacccaggtg
    11521 tgcttccagt t
//
LOCUS       DAOSYN010000023        11033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_5058_length_11033_cov_4.387411, whole genome shotgun sequence.
ACCESSION   DAOSYN010000023 DAOSYN010000000
VERSION     DAOSYN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 11033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11033
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5058_length_11033_cov_4.387411"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLJ11_01980"
     CDS             <1..684
                     /locus_tag="PLJ11_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO03470.1"
                     /translation="REVIHDFSVKVRAGQKVAIVGPTGAGKTTIVNLLMRFYEVNSGD
                     ILIDGVSTKDLTRRNVHELFCMVLQDTWLFQGTIRENIVYCKEGVPDEVVVRACKAVG
                     IHHFISTLPNGYDTVLDDESKISVGQRQQLTIARAVVQNAPLLILDEATSSVDTRTEK
                     LIQRAMDELTLGRTSFVIAHRLSTIKDADLILVMRDGSIVESGRHEELLKQKGFYSDL
                     YNSQFEGCD"
     gene            786..1724
                     /locus_tag="PLJ11_01985"
     CDS             786..1724
                     /locus_tag="PLJ11_01985"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HOO03471.1"
                     /translation="MAAPSSAPLRDSPFAHTYSIIARDRTTGEIGVAVQSHWFAVGTV
                     VPWAQAGVGAVATQALTNPAFGHKGLELMSKGVPPREAVERMVGEDEGREHRQLALID
                     AKGDSYAYTGKRCVACAGHASERDLSVQANMMLRATVWDAMASAFRRAEGPLAERMLA
                     ALEAAEGEGGDARGRQSAALVVVRGESTGMVWKDRSIDLRVDDHPEPLQELRRLLGVH
                     RAYESMDAGDAAMGAGRMEEALHHYSKAEAMLPGNAEVVFWHAVGLANIDRFEEAVPL
                     FRRAFEAEPNWKLMVPRLRTVGMLRMGEGEASRLLQ"
     gene            complement(1920..2366)
                     /locus_tag="PLJ11_01990"
     CDS             complement(1920..2366)
                     /locus_tag="PLJ11_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03472.1"
                     /translation="MAYRIPSAKVLEDSIRRVIREQQSIPSQRRFAELVLEDLHRKNP
                     EYKVGEVRLRRMALHRNLARVTIAYRETKEPSRKGRCPVCSSPTEELHNLTLDDKRVE
                     LGFKCTKCPYWTGPRKRVPVRYTFTMLGSIVPPTRKKGRYSQWKFA"
     gene            2465..3049
                     /locus_tag="PLJ11_01995"
     CDS             2465..3049
                     /locus_tag="PLJ11_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03473.1"
                     /translation="MEGERIVIEEDGTETFYVEVKDISYMLMAPPRDIFSKLSYVYMG
                     ESVVDRLLEHYRHEKGLIKRKHRLVLLMPGEKITPGMSKECEAALDRFTATKIRDNQN
                     AMAAIRRRGLLMIPYAMVFLIGSVGLGALFGTEIIPGIPSVLAQVLSEGLFIVAWVAM
                     WGPLDTLLFGRFPFRIENQALRALERTAIEVRPL"
     gene            3108..4466
                     /locus_tag="PLJ11_02000"
     CDS             3108..4466
                     /locus_tag="PLJ11_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOO03474.1"
                     /translation="MKRIKVVIMGAAGRDFHNFNTYFRDNDRYDVVAFTAAQIPNIEG
                     RCYPATLAGALYPSGIPILAEDDLRSIVRRHKVDQVVFAYSDIAHTELMHKASLVLSL
                     GADFVLMGNSAITLRSNKPVISVCAVRTGAGKSQTTRKLCRILHGRGLKVVAVRHPMP
                     YGDLTRQVVQRFGEHADLDRYDCTIEEREEYEPLLDNGIVVYAGVDYERILREAEKEA
                     DVIVWDGGNNDLPFFESNLHIVMADPHRAGHEIAYHPGEVNLRLADVVIINKVQTADR
                     RNILRVKENVLKYNPRALVIEAASPIKVDDPAMVRGKRVLVVEDGPTLTHGEMAFGAG
                     TIAAEDNGALDIVDPRPYAVGSIKELYSTYRHLGPILPAMGYTDEQIRDMEETINRAE
                     CDVVVAGTPVDLRRVMTVNKPIVRVRYELEEIGKPTLEDILDERLGPVLFACHGDSCK
                     MM"
     gene            4717..4800
                     /locus_tag="PLJ11_02005"
     tRNA            4717..4800
                     /locus_tag="PLJ11_02005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4753..4755,aa:Leu,seq:taa)
     gene            4924..5217
                     /locus_tag="PLJ11_02010"
     CDS             4924..5217
                     /locus_tag="PLJ11_02010"
                     /inference="COORDINATES: protein motif:HMM:NF020382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HOO03475.1"
                     /translation="MEVVLILFKSNLPKDKVIKNFEARADLYRAVPGLVQKYYIHDDS
                     TGHFGGIHIFDTLEAAEAFMGSDLVKSIGNAQNSHEPPTVRLLHIDLVLRNGL"
     gene            5884..6669
                     /locus_tag="PLJ11_02015"
     CDS             5884..6669
                     /locus_tag="PLJ11_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOO03476.1"
                     /translation="MDVVLLLGMVLIGLFTGAVTGLTGASGVMVVVPLVTMLLGFSIR
                     EAIGTSLMVNVISSLAISIIYYRHGNIEVRSAFWIALGSIVGAQLGTVFAGGIPEVGL
                     SGTFGIFMVIMGAVIWKKGLNHESIAKIAKGIVRFRGEAQRNVVCLVLGFAIGIMTGI
                     LGAGGGGIILLILIFVLGFPIHLAIGTSSLLMTITTLSGAIGYAMQGNIRLLPGLVLG
                     ISAAGSGVLSATFANRVDEQVLSKVVGAVFMLLGVVMTAFYIA"
     gene            complement(7216..7656)
                     /locus_tag="PLJ11_02020"
     CDS             complement(7216..7656)
                     /locus_tag="PLJ11_02020"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HOO03477.1"
                     /translation="MGLFDKFGSKEEKEKLSKEEAFAAVTLATIAADGTITEEEAQGL
                     FVHMNRMRMYSGFNDKQMSSMLNKLINIIKRKGLDELLALAKGSLPEDMRATAFAVAT
                     DLALADGDIAEEEKKLLTKVQQSLGIPEDEGVKIIEVMMIKNRG"
     gene            complement(7980..8996)
                     /locus_tag="PLJ11_02025"
     CDS             complement(7980..8996)
                     /locus_tag="PLJ11_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOO03478.1"
                     /translation="MALNQVTDVVWIVFIVSIAALLILDLGVFNRKAHVIRPREALLQ
                     MAFFISAAIVFNVGVYLYLGSTAGLEFTTGYLMELMLSVDNLFVIILIFTSFGIPRKY
                     QHKVLFYGILGALVFRLAFILVGVTLVESFEWVLYIFGAFLIYTAIKMVVGKEKGTVE
                     PERNLMVRFFRRFMRVTKDYHGDKFFVRMADDSGKHLLWATPMFIALLVIESTDIVFA
                     VDSIPAILGITTSTFIVFSSNAFAILGLRSMYFALSHIMTLFCYLKYGLAGILGFVGA
                     KMLAGIFDVHVSVLTSLLVIVVILGMAMGASVIKTRRTDACALSAKKPGKGAAKEDDG
                     EEKR"
     gene            9355..10767
                     /locus_tag="PLJ11_02030"
     CDS             9355..10767
                     /locus_tag="PLJ11_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOO03479.1"
                     /translation="MSKTKGPYVALAIVMVGVFMSVLDAVALNIALPSITSYYGVAVA
                     DTQWVVTGYLLAQTCALIISGKVAERVGQARMFTIGMVVFALSSLLCALSGSLGQLIA
                     FRLVQGLAASMMFSVSTAIVFRLFDITVRGKALGFLGSTVAVGGMLAPVIGGVLVGTL
                     GWQSIFLINVPIGIIASALAFRYLRLEERLSERLRMDWPGVVLWVLSIGSLVLMLGLL
                     GDTGTFTWEVGAYALVFIVSLALFIRWERRAEEPLLDISVFKVRRFSLIALSMVMFFV
                     SMNMVTILGPFYYEGVMVYDATTVGMIFMIMPGVTMIGAPLVGRIYDRKRFRPYTTVG
                     HLVRAGGMFLMAYAFLIKNLELTLVAFFIMGVGSCLFQTPNNSEMMLSLPRERSGLAS
                     SIQATTRNLSTAIGVSLATMLMTVLMGSMDYGAIAGGPLSNELARSVAIAVAFGGVLC
                     LVGAVLARISERTDGEASSH"
BASE COUNT         2165 a         3301 c         3444 g         2123 t
ORIGIN      
        1 agggaggtca tccatgactt ctcggtgaag gtgagggcgg ggcagaaggt ggccatagtc
       61 ggcccgaccg gagccgggaa gacgaccatc gtcaacctgc tgatgaggtt ctatgaggtg
      121 aactcggggg acatcctgat tgacggcgtt tcgacgaaag atctgacccg gaggaacgtg
      181 cacgaactgt tctgcatggt gcttcaggac acctggctct tccagggcac catcagggag
      241 aacatcgtct attgcaagga gggcgtcccc gacgaggtgg tcgtgcgcgc ctgcaaggcc
      301 gtgggcatcc atcatttcat aagcaccctc ccaaacggct acgacaccgt cctggacgac
      361 gagtcgaaga tatccgtggg gcagaggcag cagctgacca tcgcccgcgc ggtggtccag
      421 aacgcgcccc tcctgatcct ggacgaggcc acaagctccg tggacacaag gacggagaag
      481 ctgatccaga gagcgatgga cgagctcacg ctcgggcgga cgtccttcgt catcgcccac
      541 cgcctctcga ccatcaagga cgccgacctc atcctggtga tgagggacgg gagcatcgtg
      601 gagagcggaa ggcacgagga gctgctgaag cagaaagggt tctacagcga cctctataac
      661 agccagttcg aggggtgcga ctgagccctc catgttcgag cggcgggaat cgcccgggaa
      721 cgtccgttcc gttcaggaag gacgtccccc caatccttaa ttcctgacag ccgcatccaa
      781 ggcgcatggc cgctccctcc tccgcgcccc tcagggattc cccgttcgcc cacacctact
      841 ccatcatcgc ccgggaccgc acgacggggg agatcggtgt ggccgtccaa tcgcactggt
      901 tcgcggtcgg caccgtcgtc ccatgggcgc aggcgggcgt gggcgccgtg gcgacgcagg
      961 ccctcaccaa cccggccttc gggcataaag ggctggagct gatgtccaag ggggtccccc
     1021 cgcgcgaggc ggtggaaagg atggtcggag aggacgaagg ccgggagcat aggcagctgg
     1081 cgctcatcga cgcgaagggc gattcctacg cgtacacagg caaaaggtgc gtggcctgcg
     1141 ccgggcacgc gagcgaaagg gacctctcag tacaggccaa tatgatgctg cgggccaccg
     1201 tctgggatgc aatggcctcg gccttccgga gggcggaggg gcccctggcg gagcggatgc
     1261 tggcggcgtt ggaggcggcg gagggcgagg gtggagatgc caggggaagg cagagcgcgg
     1321 cgctggtggt ggtgaggggg gagagcacgg gcatggtgtg gaaggaccgc tcgatcgacc
     1381 tgagggtgga cgaccatccc gagcccctgc aggaactccg ccgcctgctc ggggtccaca
     1441 gggcctatga gagcatggac gccggggacg cggccatggg tgcgggaagg atggaggagg
     1501 cgctgcacca ttattcaaag gcagaggcca tgctgccggg gaacgcggag gtggtcttct
     1561 ggcacgcggt cggcctagcc aacatcgatc gcttcgagga ggcggtcccc ctgttccgcc
     1621 gggcgttcga agcggagccc aactggaagc tcatggtccc gcgtctgcga acggtcggga
     1681 tgctcaggat gggcgagggg gaggccagcc gattgctgca gtaggccgaa gccccggtac
     1741 ctggaaggat gacgcctccc aaccccgccg attcgaaccc ctcggagggc ctgtccgtca
     1801 ctatgccctg ttcatccagg caaagggtgg gtccaccccg gagcgcagca tcaactttca
     1861 gggctgggcg gtaatgtggg cgtaagcgct tcccgcggca tcacgaaggt catccaggcc
     1921 tacgcgaact tccattggga gtaccggcct ttcttcctgg tcggagggac gatagagcca
     1981 agcatggtga aggtatagcg cacaggcacc cgtttcctcg ggccggtcca ataggggcat
     2041 ttggtgcact tgaaccccag ctccacccgc ttgtcgtcaa gggtgaggtt gtgcagctcc
     2101 tcggtggggg aggagcagac ggggcagcgc cccttgcggc tgggctcctt cgtctcccga
     2161 taggctatgg tgaccctggc caggttgcgg tgcaaggcca tcctcctcaa ccgcacctcc
     2221 cccaccttgt attcggggtt cttcctgtgg aggtcctcca gcacaagctc ggcgaagcgg
     2281 cgctgcgaag gaatgctctg ctgctcccgg atgacccttc ttatcgagtc ctcgaggacc
     2341 ttggccgagg ggatgcggta ggccatccgt gctgaatcgg tgcgtgcgta ctcaaagctt
     2401 tgctatgccc ggcagcaaaa ggggcagcaa catcatttat ttcagaacgc gatgtggaag
     2461 gggcatggaa ggggaacgaa tcgtcataga ggaggacggg acggagacgt tctacgtgga
     2521 ggtcaaggac atcagctaca tgctcatggc ccctcccagg gacatcttct ccaagctgtc
     2581 ctacgtgtac atgggcgaat cggtggtcga tcggctcttg gagcactatc gccatgagaa
     2641 gggcctcatc aaaaggaagc accggctggt tcttctgatg cccggggaga agatcacccc
     2701 ggggatgtcc aaggagtgcg aggcggccct ggaccgcttc accgccacga agatcaggga
     2761 caaccagaac gccatggccg cgatccgccg ccggggcctg ctgatgatcc cctacgccat
     2821 ggtgttcctg atcggctcgg tgggcctcgg ggcccttttc ggcacggaga tcatcccggg
     2881 catcccttcg gtgctggcgc aggtgctcag cgaggggctg ttcatcgtgg cctgggtggc
     2941 catgtggggg ccgctggaca ccctgctgtt cgggcgattc cccttccgga tcgagaacca
     3001 ggcgctgcgg gcattggaaa ggaccgctat cgaggttcgc cccctctgat gggctcccgc
     3061 ggaaaatgta atagaggtgg cattgattcg ggtagaggtc catgcacatg aaaaggataa
     3121 aggtagtgat catgggcgcc gctgggcgcg acttccacaa cttcaacacc tatttccgtg
     3181 acaacgacag gtacgacgtg gtcgccttca ccgccgctca gatccctaac atagaggggc
     3241 ggtgctaccc tgccaccctg gcaggggcgc tgtacccctc cggcatccct atactggcgg
     3301 aggacgacct tcgatcgatc gtccgcaggc acaaggtgga ccaggtggtg ttcgcctaca
     3361 gcgacatagc gcacaccgag ctcatgcaca aggcctcgct ggtgctgtcc ctcggggcgg
     3421 acttcgtact catgggcaac agcgcgataa ccctccgcag caacaagccc gtcatctccg
     3481 tctgtgcggt acgcaccggg gcgggcaagt cccagaccac caggaagctg tgccgcatcc
     3541 tgcacgggcg cggtctgaag gtggtcgcgg tccgccatcc catgccctac ggcgacctga
     3601 caagacaggt ggtgcaacgt ttcggcgagc acgccgacct ggacaggtac gattgtacca
     3661 tagaggagag ggaggagtac gagccgctgc tcgacaacgg tatcgtcgtg tacgcgggcg
     3721 tggattacga gcgcatcctg cgggaggcgg agaaggaggc ggacgtcatc gtctgggacg
     3781 gggggaacaa cgacctgccg ttcttcgaga gcaatctgca catcgtgatg gcggacccgc
     3841 atcgtgccgg tcatgagatc gcctatcacc caggcgaggt gaacctgcgc ctggcggacg
     3901 tcgtgatcat caacaaggtg cagaccgcgg accgcaggaa catcctcagg gtcaaggaga
     3961 acgtgctgaa gtacaacccc agggcgctgg tcatcgaggc cgcctcgccc atcaaggtcg
     4021 acgacccggc catggtcagg gggaagcggg ttctggtcgt ggaggatggc cccacgctca
     4081 cccacgggga gatggccttc ggcgccggga cgatcgcggc ggaggacaac ggcgccctgg
     4141 acatagtgga cccgaggccg tacgcggtcg gctccatcaa ggagctttac tccacttacc
     4201 ggcacctggg tccgatcctc ccggccatgg gctatacgga cgagcagatc agggacatgg
     4261 aggagacgat caacagggcc gagtgcgatg tcgtggtggc ggggacgccc gttgacctcc
     4321 ggagggtgat gacggtcaac aagcccatcg tccgcgtgcg ctacgagctt gaggagatag
     4381 gcaaacctac tctggaggac atcctggacg agaggctggg accggtgctc ttcgcctgcc
     4441 acggcgattc ctgcaagatg atgtgagcgt ccccaatacc ccggtccttg gccggagggc
     4501 ggggggtggg cagcacctcc ctctcgccgg gatcgcatga tgcacgccgc ttcgccatcc
     4561 atccggaggc tgaaggacct taacggcgcc tttctctttc cttaatgata cgcccctgga
     4621 tatgatggac cggggagttc gacgaagccc ctcggggcgt tcatctgtcg ctcttttgac
     4681 aaacttatta tgctgtatcg accatggccg aagcgcgcgg gggtagccaa gcttggccaa
     4741 cggcgcagga cttaagatcc tgtccttagt ggttcaaggg ttcgaatccc ttcccccgca
     4801 cctccatcca agaaaaaatg catcttcagt tttctccgga tctctgaacg ctctgaattc
     4861 aaggtcgata tcctatgaga acgcttatca tccctccgtg tcatcgatgg taggggtcat
     4921 tccatggaag tggttctcat cctgttcaag agcaatctgc cgaaggacaa ggtcataaag
     4981 aacttcgagg caagggccga cctgtaccgc gcagttcctg gcttagtgca aaaatactac
     5041 atccatgacg attctacagg tcattttggc ggcatccata tttttgatac tctagaggcc
     5101 gcggaggcgt tcatgggctc ggacctggtg aagagcatag gcaacgcaca aaattcccat
     5161 gagccgccga ccgtaaggct cctccacata gatctggttc tccgcaacgg gttatgatcc
     5221 gcaacgcagc cattgcacac cggttccatc catgccccat ctcgccatat ggtgtgctgg
     5281 ggaacattat agaacaacct ttcatttccg agcgctctta atgatcatgg cagcatgact
     5341 taggatcccg tccttagtgg ttcaagggtt cgaatccctt ccccgcactt atcttctaat
     5401 gtactggcta gcgccctcgc ttaggaatta ttcgaataga attcaaatta tcaagcttga
     5461 ctaccttaca gatttttggt actgtcatgt tcgtagggag tatggtcata ggaagtatgg
     5521 acatgagagg aaaattggac acggcctatt gtctaggtag catactcgga caatggccga
     5581 tgggtcttcc aaacgccacc aggttacgac aactggcgat gtgcgtgttc gattccaacc
     5641 acaacaagtc ataccagagg aaatgcctca tcgccatcga acgacatgcg atgttcctgc
     5701 tggtcgaagg ggtcaagctt aagaagcctg gggaggcgca acaaggccct gatcgatcag
     5761 atcaatttat cgcaatgcta cacaagcatc ccgctgcatg aaagcggcgc gcctcccgat
     5821 agaaaccttt ttatattggc tgccttctat aaaatagaag ctcccagggg gcggagggga
     5881 aagatggacg tcgttctgtt attggggatg gtcctcatcg ggctcttcac cggggcggtg
     5941 accggcctca caggcgcgtc cggggtgatg gtggtcgtgc ccctggtcac catgctgctg
     6001 ggcttctcga tccgcgaggc cataggaacg agcctgatgg tgaacgtcat ctcctccctg
     6061 gcgatatcga tcatctacta taggcatggg aacatagagg tcaggtccgc gttctggatc
     6121 gctctcgggt cgatcgtcgg cgcacagctg ggaacggtct tcgctggcgg aataccggag
     6181 gtgggcctga gcggaacgtt cggcatcttc atggtgatca tgggcgcggt catatggaag
     6241 aagggcctga accacgagtc catagcgaag atcgccaaag ggatcgttag gttcaggggc
     6301 gaggcccaga ggaacgtggt atgcctcgtc ctggggttcg ccatcggcat catgacgggg
     6361 atccttggcg caggaggagg agggataatc ctgctcatcc tcatcttcgt cctgggattc
     6421 ccgatccacc tggccatagg cacctcctcc cttctgatga cgataaccac cctttccggg
     6481 gccatcggat acgcgatgca ggggaatatc cggctcctcc ccggtctggt ccttgggatc
     6541 agcgcggcag gcagcggtgt gctcagcgcc actttcgcca acagggtcga cgaacaggtc
     6601 ctgtccaagg tcgttggggc ggtgttcatg ctgctcggcg tggtcatgac cgcgttctat
     6661 attgcctgac ctcggagagg gctcgcattc aaggcccctc cccccaggga cctaagaccg
     6721 ataggcaccg atcgaagcgc cgaggctcga gaaacctccg ttctttttct cataagagag
     6781 ggcgggacgc gcccaaggcc aatggcccac ttatgccgtg cttcgagccc cgccggcaat
     6841 ccttcctaat gagcggccca ccaagcgtag cgtgatcagg cgcgcccggt actctgtaca
     6901 catcccatgc ccgtctccga gccatcccca tctttcgtgc agcccgatcg ctccttgagg
     6961 ccgaaggcct gcgccgagtc cgggcaagga tcgatgtcct gcagcttaag ggtccgtaag
     7021 ggcatcggtc cgcctgccac tccaacgaac ggatgacaga gtgacggcac tcgccttcat
     7081 cgaaggcccg atgcgatata ggggcggctg gcatcgagcg caagcattga cggggggccc
     7141 tcctccattc agcgctccag cgcagatccc cctccaaggc gatcaagtgg gtcgaaaagc
     7201 gtttgaaggg ggcgctcagc cccggttctt gatcatcatg acctcgatga tcttgacccc
     7261 ctcgtcctcc gggatgccga gggactgctg gaccttggtc agcagcttct tctcctcctc
     7321 ggcgatgtcc ccgtcggcca gggccaggtc ggtggccacg gcgaaggcgg tggccctcat
     7381 gtcctcgggc agggacccct tggccagggc caggagctcg tccaacccct tccgcttgat
     7441 gatattgatg agcttgttca gcatcgacga catctgcttg tcgttgaagc ccgagtacat
     7501 cctcatcctg ttcatgtgga cgaagaggcc ctgcgcctcc tcctccgtga tcgtgccatc
     7561 ggcggctatg gtagccaggg tgacagcggc gaacgcctcc tccttgctca gcttctcctt
     7621 ctcttccttt gaaccgaact tgtcgaacag acccatggtc caacctcctt tttgatctct
     7681 catggcggtg cctggacaag gtccagggac cgttcccagg cctttccttt caggcccggg
     7741 caggaagggc gatccgctct cctgcaataa gcctatctgt tgtacaacgc ggggaggatg
     7801 cccagtcccc tggtcctgtt cgcgcatcat cgaatgtcat ccatctgtgc ccacgtcctt
     7861 cggtccacgg tccgaagacc gataccctcc ccctcatcag ggtcattggc ggacgatcgc
     7921 acgtccggag cgcggaacgg ggcccaaggg aatggtgaaa taaggaggga gagatctcct
     7981 caccgctttt cttccccgtc atcctctttg gcagccccct taccaggctt cttcgccgac
     8041 agcgcgcagg catcggtccg gcgcgtcttg atcaccgagg cgcccatggc catccccagg
     8101 atgaccacga tgacgagcag agaggtcagc acggacacgt gcacgtcaaa gatgcctgcc
     8161 agcatcttcg ccccgacgaa ccccaggatg ccagccagcc catacttcag atagcagaac
     8221 agggtcatga tatgggacag ggcgaagtac atggacctca gcccgagaat ggcgaaggcg
     8281 ttggagctga acacgatgaa ggtggaggtc gtgatgccca gtatggctgg gatggagtcc
     8341 acggcgaaca cgatgtccgt gctctcgatg accaggaggg cgatgaacat cggtgtggcc
     8401 cagagcaggt gcttgccact gtcatccgcc atgcgcacga agaacttgtc accatgatag
     8461 tccttggtca ccctcatgaa cctgcggaag aagcggacca tcaggttgcg ctcgggctcc
     8521 acggtcccct tctcctttcc gaccaccatc ttgatggcgg tgtagatcaa aaaggccccg
     8581 aagatgtaca gcacccattc gaacgactcc accagcgtga ccccgaccaa tatgaacgcc
     8641 agcctgaaga ccaacgcccc caggatgccg tagaagagga ccttgtgctg atacttcctc
     8701 ggtatgccga aggaggtgaa gatcaggatg atgacgaaca ggttgtccac gctgagcatg
     8761 agttccatga ggtacccggt ggtgaactcc aggccagcgg tggagcctag gtagagatac
     8821 actcccacat tgaagacgat ggcggcgctg atgaagaacg ccatctgcag cagggcctcc
     8881 ctcggcctga tcacatgggc cttgcggttg aagaccccca gatcgaggat cagcagggcg
     8941 gcgatgctga cgatgaagac tatccacacc acatcggtca cttggttcaa ggccaccagt
     9001 cgtctcccgg gagggcgacg ggcatcgccc ctcccagtga aattgcgatg aagacgcaaa
     9061 acgggcattc gtatataggc ttattcgtct gaacgcccca ggggtagtgg aaccgatggg
     9121 tcatggcccg cgggtcgggc gccttcaacg gaccatagga tgctcgggaa acggtcaatt
     9181 ccctgcgttc cgcctttcct cacggccgtc ccagctaggc caaagaggcc cgtcccatgc
     9241 aggttcccag gtggacgtgc ttcgttcgtc ccgcgcggcc cctgtccctc caccatccgt
     9301 acacttcgtt cataaccttt aagggggcgc cctcgttcat ggggtccgag cacgatgagc
     9361 aaaacgaagg gaccgtacgt cgccctcgcc atcgtgatgg taggcgtgtt catgtccgtc
     9421 ttggacgcgg tggccttgaa catcgccctg ccatcgatta cgagctatta cggcgtggca
     9481 gtggcggata cgcaatgggt ggtcactggc tatcttctgg cacagacctg cgcactgata
     9541 atctcgggca aggtggccga gcgggtgggc caggcgagga tgttcaccat cgggatggtg
     9601 gtcttcgccc tgtcctccct gctctgcgcc ctctccggga gcctcgggca gctcatcgcc
     9661 ttccgattgg tgcaggggct ggccgcatcg atgatgttca gcgtctccac cgccatcgtg
     9721 ttccggctgt tcgacatcac cgtcaggggg aaggcgctgg gcttcctggg ctccacggtg
     9781 gcggtcggcg gcatgctcgc cccggtgatc ggcggggtac tggtgggcac cctgggctgg
     9841 cagtcgatct tcctgatcaa tgtgcccatc ggcatcatcg cctccgccct ggccttcagg
     9901 tacctccggc tggaggagag gttgagcgaa cgcctgcgca tggactggcc tggggtggtg
     9961 ctctgggtgc tctccatcgg ctcgctggtc ctcatgctcg gcctgctggg ggacaccggg
    10021 acgttcacct gggaggtcgg ggcgtacgca ctggtcttca tcgtctccct cgccctgttc
    10081 atccgctggg aaaggagggc ggaggagccg ctcctggaca tctcggtctt caaggtgcgg
    10141 aggttctccc taatcgccct cagcatggtc atgttcttcg tctccatgaa catggtcacc
    10201 atcctcgggc cgttctacta cgagggggtg atggtctacg acgcgacaac cgtgggcatg
    10261 atattcatga tcatgcctgg ggtaacaatg ataggcgccc ccctggtcgg aaggatatac
    10321 gaccgcaagc gcttccgccc ctacacaacc gtcggccatc tggtgcgggc cggggggatg
    10381 ttcctcatgg cctacgcctt cctgatcaag aacctggagc tcacgttggt ggccttcttc
    10441 ataatggggg tcgggagctg cctgttccaa acgcccaaca acagcgagat gatgctctcc
    10501 ctcccgaggg agaggagcgg gctggcctcc agcatccagg ccaccacccg gaacctcagt
    10561 acggcgatcg gcgtgtcctt ggccaccatg ctcatgacgg tcctgatggg ctcgatggac
    10621 tacggggcga tcgccggggg gccgctctca aacgagctag cgaggtcggt cgctatcgcc
    10681 gtcgccttcg gaggggtgct atgcctcgtc ggagctgttc tggcccgcat aagcgaaagg
    10741 acggatgggg aggcctcctc ccattgagcc cggcgcaacg atcaccgcag ttggtccaaa
    10801 ttcaaaggcc gctcctgcgg tcaggggcgt caatgggggc ctgggcagca ttttgatcgg
    10861 tcaggcactc ccctccgctc ctccagaagg gccatcatac aggtctttag tgaagaccga
    10921 gcggaacgct gggccctaat gacagcacca gagagggtgg cgatcccccg gccaatcggg
    10981 ccaaggacgg gtaaaaagaa atagaagggg gccgttggcg cttgccatgc cct
//
LOCUS       DAOSYN010000024        10676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_5284_length_10676_cov_3.977497, whole genome shotgun sequence.
ACCESSION   DAOSYN010000024 DAOSYN010000000
VERSION     DAOSYN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5284_length_10676_cov_3.977497"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLJ11_02035"
     CDS             <1..253
                     /locus_tag="PLJ11_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HOO03480.1"
                     /translation="QALSLPTAPLRPIIVRREEDRPQPIADALAGEPERARGMAVSVG
                     RVRERKGYIKLWVLSHNTLRGGAGGSVLNAELAKAKGLL"
     gene            266..1432
                     /locus_tag="PLJ11_02040"
     CDS             266..1432
                     /locus_tag="PLJ11_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOO03481.1"
                     /translation="MKRTVAEINQRIKDGDVVVMTAEEVVSYVQSEGVERAAKEVDVV
                     TTGTFGAMCSSGAFLNFGHSEPPIRMQRVTLNDVPAYAGLAAVDAYIGATELRQDGRE
                     EYGGAHVIEELVAGRAVRLKASSSGTDCYPRKEIDTYVSLNTMNQAYLFNPRNSYQNY
                     ASGTNSSERTLYTYMGKLLPNYGNITYSSAGQLSPLLNDPTLRTIGIGTRLFLGGGTG
                     YVAWEGTQFKTNVETRFGVPMGGARSLATIGDMRGMSPEFIRAVALTGYGVSLAIGIG
                     VPIPILDEEVMRSCAISDEQLFSPLVDYSVQSRNRRPIKHFSYAELRSGKVELFGREV
                     RTSSLSSYLKARQVAETLAERIEGGEFLLTEPVAPLPADRGVGTLEVRSKEEAV"
     gene            1432..1836
                     /locus_tag="PLJ11_02045"
     CDS             1432..1836
                     /locus_tag="PLJ11_02045"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO03482.1"
                     /translation="MAKRKFLLGFSPDLVNEAIVYRLVKDYDLKINILRAEVREQGGR
                     LLMEVEGKAGNIKEAVRYLNEARVEVQELATYVEKNEERCTHCGMCLSICPVEALTVE
                     RPSWRVRYDAERCIACGMCIDACPPGAMKFRL"
     gene            1833..2552
                     /locus_tag="PLJ11_02050"
     CDS             1833..2552
                     /locus_tag="PLJ11_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOO03483.1"
                     /translation="MRRHFQYKETAVTIEADEEYLPLAEKAVLDAREEVEAYIAKDPY
                     FKATFDPYPVRADMGEVVRHMCEAAQRADVGPMAAVAGAIGHHAVAAMTKAGCKHCLV
                     DNGGDIVMRTNRTVTVGLYSSVRTPNYLALEVPATKAPYSICTSSGTVGPSISLGRAD
                     LATVIAADGALADACATKLGNLITEDDLTLIDRAIREVLAIEGVKGALVMIAGKLGLG
                     GEVPWLVRCDVPPELITRVRY"
     gene            2697..3560
                     /gene="hisG"
                     /locus_tag="PLJ11_02055"
     CDS             2697..3560
                     /gene="hisG"
                     /locus_tag="PLJ11_02055"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOO03484.1"
                     /translation="MTLRLAIPNKGRLSERSIEILKQAGLEIENGDERRLYANVKRQD
                     LVVMFLRAQDIVRFVHKGAVDVGITGYDLVLESGLDVVVPYRLNFGHCRLSVAVPDTM
                     AVKGPEALPDGLVVATSFPNLTRRYFEGLGKRVEIAPIAGAAEVAPLLGVADIIVDLV
                     ASGSTLKMNHLRELAVIAESQAVVIANREAYEGCEQQVQEVISAMLSVTEAENKKYLM
                     ADVPKASLEEVKRAFPGIAGPTIMNIIGRDDVVAVHVVIDKADAYDAINQLKRMGATG
                     ILLTPIDRMVP"
     gene            3570..>4228
                     /locus_tag="PLJ11_02060"
     CDS             3570..>4228
                     /locus_tag="PLJ11_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO03485.1"
                     /translation="MDRNWIRSTCRDLQLYYNPEVGNKLRMDTSTNALGANPAGMRAL
                     RECCDLDLGQYPSTYGDGLREELARVHRLSRDNFVVGNGSDESLDVIMKTLVEPGGTV
                     ITAHPAYVLHSFFVSINGGRTVTVDLDERFQLDVERINSTPGSLVLICTPNNPTSNTF
                     RDEDVRAVIEGAARPVVVDEAYAEYAGRSFLPLVERYDNLIVTRTFSKASSAVFDARC
                     P"
     assembly_gap    4229..4238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4239..4683
                     /locus_tag="PLJ11_02065"
     CDS             <4239..4683
                     /locus_tag="PLJ11_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO03486.1"
                     /translation="RTFSKAYGLAGMRVGYLAAGRELADALQRVKVPYSLNKVGERVA
                     IAALRDPAFLEESVRVVAEQRPFLEEGLRALGFQVFPSEANYILFRPTVPSERLTKGL
                     ADRGVLVRDFGKVRRLENCVRTTIGTREMNTVLLERMQEVLRECR"
     gene            4674..5273
                     /gene="hisH"
                     /locus_tag="PLJ11_02070"
     CDS             4674..5273
                     /gene="hisH"
                     /locus_tag="PLJ11_02070"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOO03487.1"
                     /translation="MPLRIELADYGVGNLHSIRKALENCGAEVHVAEDMALLERAECI
                     VLPGVGAFDRAMERLRPHGEAIVERLDEGVPCLGICIGMQVLFQRSAEGASPGLGFLE
                     GEVVRLSAPRVPHMGWNEVVTADAIMDGIEDRHFYFAHSFRGRPTDGSAVGHTDHFGS
                     FPSLFRKRNVYGTQFHPEKSSSAGLAFLRNFVRFAEGCA"
     gene            5270..5971
                     /locus_tag="PLJ11_02075"
     CDS             5270..5971
                     /locus_tag="PLJ11_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HOO03488.1"
                     /translation="MIVVPAVDILDHRVVQLVGGVPGTERVVLPDPVAVAEGWEAEGA
                     RRLHVVDLDSALGKGHNAQAIRRIVQGAGIPVQVGGGIRSSEVVEYYLDCGADRVIVG
                     TKGLKDPAWLRRMAERRPGRLVLALDLRGGRVQAQGWREGLPLGLEDVFATIADMPLA
                     GVLYTNVDVEGQGAGIDAGAVRAFMAACPHPTIVSGGITTERDLEALEGMGVREAVVG
                     LALYTGKLNHSVIRR"
     gene            5976..6527
                     /locus_tag="PLJ11_02080"
     CDS             5976..6527
                     /locus_tag="PLJ11_02080"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOO03489.1"
                     /translation="MDRKTEVVRKTRETEVRLKLDLDGEGNADVRCEEQFLRHMVGTL
                     ARYADFDLALEAKGDDVHHLVEDVAIVLGKTLKQCLGESPIERTAFSLIPMDDALVQV
                     AVDIIERPYADVDCPNALYAHFLRSFAMSSGITLHVMVLRGYDEHHIVEASFKALGKA
                     LRAAVRPRRSELSTKDRPKVSTE"
     gene            6524..7279
                     /gene="hisF"
                     /locus_tag="PLJ11_02085"
     CDS             6524..7279
                     /gene="hisF"
                     /locus_tag="PLJ11_02085"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOO03490.1"
                     /translation="MSLTKRIIPCLDVKDGAVVKGVRFLDLKDVGSPPAMAEEYERQG
                     ADEIVFLDISASVEGRRTLLGVVEETAKRLFVPLTVGGGIRETADVRAALNAGADKVS
                     INTVAVQRPEIISECAKDFGSQCVVVAIDAKRRAGGWTVYTHGGRRDAQLDMVRWAKR
                     VEELGAGEILLTSMDADGTKDGYDIAMTRAVADAVSIPVIASGGCGSPRHILEVLRET
                     KAEAALAASIFHYGEWTVGDVKEYLRRNGVEVR"
     gene            7276..7878
                     /gene="hisIE"
                     /locus_tag="PLJ11_02090"
     CDS             7276..7878
                     /gene="hisIE"
                     /locus_tag="PLJ11_02090"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOO03491.1"
                     /translation="MSAPKFDADGLVPVIVQDHETNEVLMMAYANEQAYRDMLRTGYT
                     HFYSRSRQAQWRKGETSGHVQEIVSIQLDCDGDALLVRVRQTGVACHLGRMSCFDEVL
                     YGDPDGTMAVLPPLLRVIRERRAEPREGSYTCALFADENKRLKKVVEEAGEVLLAAKD
                     RKAEEQAWEVADLVYHLLVAVEGMEVTWEDVYRKLAERRR"
     gene            7875..8534
                     /locus_tag="PLJ11_02095"
     CDS             7875..8534
                     /locus_tag="PLJ11_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOO03492.1"
                     /translation="MRAELHTHTLLSDGELLPIELARRAVVKGTKYLAFTDHVSLSNL
                     ERVVAEGRRDAELAEEWGMKVLAGVEVTHVPVRRIDEVVLKARRLGAELVVVHGETLS
                     EPVEKGTNMAAVLNPEVDILAHPGLITLEEAQLARDNGVALEITSRSSHCKANGHVAR
                     MAQQAGAPMVVNTDAHGPDDLIDLPTALQLALAAGLTREEADRALIEVPKAIIKRRWR
                     G"
     gene            8522..9886
                     /locus_tag="PLJ11_02100"
     CDS             8522..9886
                     /locus_tag="PLJ11_02100"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOO03493.1"
                     /translation="MARLKVLKFGGSSLRTGGSIRQVAGIIMAEREKKAVVLSAVTGV
                     TEMLVQFISRTRSEEDVDAFMREITKLHVDLARDALRTESARAKAVEELVAKLVKLER
                     MLYGSCYIEEVNPRIRDYVQCFGERLSVIILSALLNELGERARPVDADELGIITDGLY
                     GAATVNMEATRASIRPKLLRMLQEDTTPVITGFFGRAPEGSVTVFGRNGSDYSAAAVA
                     NAMGASSLEIWKDVDGFMSVDPKIVPSAKPIPRLSYDEAAELAYFGAKVVHPMTMEPA
                     RSGNIEIRVKNVFRPEAPGTAIGGADNVDQSIKSISCMKDLSLVKVFGEGTGYKLGVL
                     ADVASLLRRAEINIYSVVTSLTCIALLIDRRDVAKAQKVLAESGASRVETHTGFALMC
                     IVGEGLGHHKGLASRVFKAVAKEGVNVGLISAGASLVAYHFTVDEADLERATRAVHEE
                     LFGC"
     gene            9888..>10676
                     /gene="pheA"
                     /locus_tag="PLJ11_02105"
     CDS             9888..>10676
                     /gene="pheA"
                     /locus_tag="PLJ11_02105"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOO03494.1"
                     /translation="MIIAFQGVRGAYSEDAVRAWDEGAEALECPEFIDLFDAVRSGRA
                     DLGMLPVENSIEGSVTAANDLLFRSDLWVVGEVMVRIRHCLIGHPGAGVQDVRRVYSH
                     PQALAQCRDLLTSHPEWEKVPAYDTAGSVRMVKERGLREEAAIASRRAAETYGMEVLM
                     EDVQSVRDNITRFFVLSRERGTGRGDKTALAFTTKNRPGALFDCLRAFADNGVNITKL
                     ESRPWRGRAWEYVFYLDLDGDVEDERVAAALRALRESASCVKVFG"
BASE COUNT         1798 a         3336 c         3988 g         1544 t
ORIGIN      
        1 ccaggcgctc agccttccca ccgcccccct ccggccgatc atcgtgcgca gggaggagga
       61 ccgcccccag cccatcgcgg acgccctggc gggggagccc gagcgggcga gggggatggc
      121 cgtgagcgtg ggccgtgtcc gggagcgcaa aggatatatc aagctctggg tgctttccca
      181 caacaccctg aggggcgggg ccggcgggtc cgtgctcaac gcggagctgg caaaggccaa
      241 ggggctcctc tgaggggggt gctggatgaa gcggacggta gcggagatca accagaggat
      301 caaggacggg gacgtggtgg tcatgaccgc cgaggaggtc gtctcctacg tccagagcga
      361 gggcgtggag cgcgccgcca aggaggtgga cgtcgtgacg acgggcacct tcggggccat
      421 gtgctcctcc ggcgccttcc tgaacttcgg ccattccgag ccgccgatcc gcatgcagcg
      481 ggtgaccctc aacgacgtcc cggcctacgc cgggctggcg gcggtggacg cctacatcgg
      541 cgccaccgag ctcaggcagg acgggcgcga ggagtacggg ggcgcgcacg tcatcgagga
      601 gctggtggcc ggaagggcgg tccgcctcaa ggcctcctcg agcggcaccg actgctaccc
      661 acgcaaggag atcgacacgt acgtctcgct gaacaccatg aaccaggcgt acctcttcaa
      721 cccccggaac tcgtaccaga actacgcctc ggggaccaac tcctccgagc gcaccctcta
      781 cacctacatg ggcaagctgt tgccgaacta cggcaacatc acctattcca gcgccgggca
      841 gctgtccccc ctcctcaacg accccacgct gcgcaccatc ggcatcggca cccggctgtt
      901 cctgggcggg gggacggggt acgtggcgtg ggagggcacg cagttcaaga ccaacgtgga
      961 gacgcggttc ggggtgccca tgggcggggc gaggagcctc gccaccatcg gggacatgcg
     1021 gggcatgtcc cccgagttca tccgggcggt ggccctcacc gggtacggcg tgtccctggc
     1081 catcgggatc ggggtcccca tcccgatcct ggacgaggag gtcatgcgga gctgcgccat
     1141 cagcgacgag cagctcttct ccccccttgt cgactactcc gtccaatccc ggaaccgcag
     1201 gccgatcaag cacttctcct acgcggagct gcgctcgggg aaggtggagc tcttcggccg
     1261 ggaggtgcgg acctcctccc tctccagcta cctgaaggcg aggcaggtcg ccgagacgct
     1321 cgcggagcgg atcgagggcg gggagttcct gctcacggag ccggtggcgc cgctgcccgc
     1381 ggacaggggg gtcggcaccc tggaggtgcg gtccaaggag gaggcggtct gatggccaag
     1441 cggaagttcc tcctgggctt cagcccggac ctagtgaacg aggccatcgt ctaccgcctc
     1501 gttaaggact acgacctgaa gatcaacatc ctccgggcgg aggtgcggga gcagggcggc
     1561 cggctgctga tggaggtgga ggggaaggcg ggcaacatca aggaggccgt ccgctacctg
     1621 aacgaggcga gggtggaggt gcaggagctc gccacctacg tggagaagaa cgaggagcgg
     1681 tgcacccact gcggcatgtg cctgtcgatc tgcccggtgg aggcgctcac ggtggagagg
     1741 ccgtcgtgga gggtccgcta cgacgccgag cggtgcatcg cctgcgggat gtgtatcgac
     1801 gcctgccctc cgggagcgat gaagttccgg ctatgagaag acatttccag tacaaggaga
     1861 cggccgtcac gatcgaggcg gacgaggagt accttcccct ggcggagaag gccgtgctcg
     1921 acgcccgcga ggaggtggag gcgtacatag ccaaggaccc ctacttcaag gccacgtttg
     1981 atccctatcc cgtgagggcg gacatggggg aggtggtccg gcacatgtgc gaggccgcgc
     2041 agcgggcgga cgtggggccg atggccgcgg tggcgggcgc catcggccat cacgccgtgg
     2101 cggcgatgac gaaggcggga tgcaagcatt gcctggtgga caacggcggg gacatcgtca
     2161 tgcgcaccaa tcggaccgtg accgtcggcc tgtactccag cgtccggacg ccgaactatc
     2221 tggcccttga ggttccggcc acgaaggcgc cgtacagcat atgcacctcg tccggcacgg
     2281 tcggaccgtc catatccctg ggcagggcgg acctggcgac cgtcatcgcc gcggacgggg
     2341 cgctcgcgga cgcctgcgcg accaagctcg gcaacctgat caccgaggac gacctgacgc
     2401 tcatcgaccg ggccatccgg gaggtgctgg ccatcgaggg ggtcaagggc gccctggtca
     2461 tgatcgccgg gaagctgggg ctgggagggg aggtcccctg gctggtgcgg tgcgacgtcc
     2521 ccccggagct catcacccgc gtccgctact gatgtcccga tctgggacat gcgttgaggg
     2581 cgctcatggg ggactgaagc tggcaaaggg ccccgggcgg gcgcccgcgc ccgaccagag
     2641 tgtcagtagt tataagtagc aattgcccat tgatggggag tgccaagggc tatcttatga
     2701 cactcaggct ggccataccc aacaaaggac ggctgagcga gaggtccata gagatcctca
     2761 agcaggccgg gctggagatc gagaacggcg acgagcgcag gctctacgcc aacgtcaagc
     2821 gccaggacct ggtggtcatg ttcctccggg cccaggacat agtccggttc gtgcacaagg
     2881 gggcggtgga cgtgggcatc accggctacg acctcgtgct cgagtccggg ctggacgtgg
     2941 tcgtccccta caggctcaac ttcgggcact gccgattatc cgtggcggtc cccgacacca
     3001 tggccgtcaa ggggccggag gccctcccgg acgggctggt ggtggccacc tccttcccga
     3061 acctcacccg caggtacttc gagggtctgg gcaagcgggt ggagatcgcc cccatagccg
     3121 gggcggcgga ggtcgccccg ctcctggggg tggcggacat catcgtcgac ctggtggcca
     3181 gcggctccac tcttaagatg aaccacctgc gggagctcgc cgtgatcgcg gagtcgcagg
     3241 cggtggtcat cgccaaccgc gaggcctacg aggggtgcga gcagcaggtc caggaggtga
     3301 tctcggccat gctgagcgtg accgaggcgg agaacaagaa gtacctcatg gccgacgtgc
     3361 ccaaggcctc cctggaagag gtcaagcggg ccttccccgg gatcgccggg ccgaccatca
     3421 tgaacatcat cggccgggac gacgtggtgg ccgtgcacgt cgtcatcgac aaggcggacg
     3481 cgtacgatgc gatcaaccag ctgaagcgca tgggcgccac cggcatcctg ctgaccccca
     3541 tcgaccggat ggttccctga ggtgcgtcga tggacaggaa ctggatccgc tccacgtgcc
     3601 gggaccttca gctctactac aaccccgagg tggggaacaa gctgcgcatg gacacgagca
     3661 ccaacgccct gggcgccaat cccgcgggga tgcgggcgct ccgggagtgc tgtgacctgg
     3721 acctggggca gtaccccagc acctacggcg acggtctcag ggaggaactg gccagggtgc
     3781 accggctgag cagggacaac ttcgtcgtgg gcaacggctc cgacgagtcc ctggacgtga
     3841 tcatgaagac gctggtggag cctgggggga cggtcatcac cgcccacccg gcctacgtgc
     3901 tgcactcctt cttcgtcagc atcaacggcg ggaggacggt cacggtcgac ctggacgagc
     3961 ggttccagct ggacgtggag cgcatcaact ccacgccagg gagcctcgtc ctcatctgca
     4021 cgccgaacaa ccccacctcc aacaccttcc gggacgagga cgtgcgggcg gtgatcgagg
     4081 gggcggcccg cccggtggtg gtggacgagg cgtacgcgga gtacgccggg cgctccttcc
     4141 tccccctggt ggagaggtac gacaacctga tcgtcacccg gacgttctcg aaggccagct
     4201 cggcggtgtt cgacgcccgc tgccccatnn nnnnnnnncc ggacgttctc gaaggcgtac
     4261 gggctggcgg ggatgcgcgt cggctacctc gccgccggca gggagctggc cgacgccctg
     4321 cagagggtca aggtcccgta ctcgctgaac aaggtcgggg agcgggtggc catcgccgcc
     4381 ctcagggacc ccgccttcct cgaggagagc gtgcgcgtcg tcgccgagca gcggcccttc
     4441 ctcgaggagg ggctgcgcgc cctcggcttc caggtgttcc cgtcggaggc caattacatc
     4501 ctcttccggc cgacggtccc ctcggagagg ttgacgaagg ggctcgcgga caggggcgtg
     4561 ctcgtccggg acttcgggaa ggtccggcgc ctggagaact gcgtgcgcac caccatcggc
     4621 acccgggaga tgaacaccgt cctgctggag aggatgcagg aggtgctgag ggaatgccgc
     4681 tgaggatcga gcttgccgat tacggcgtgg ggaacctgca cagcatccgg aaggccctgg
     4741 agaactgcgg ggccgaggtc cacgtggcgg aggacatggc cctcctggaa cgggcggagt
     4801 gcatcgtcct gccgggggtg ggggccttcg accgggcgat ggagcggctg cgcccccacg
     4861 gggaggccat cgtggagagg ctggacgagg gcgtcccctg cctggggata tgtattggca
     4921 tgcaggtcct gttccagagg agcgcggagg gcgcctcgcc cggcctcggg ttcctggagg
     4981 gggaggtggt ccggctgagc gccccgcgcg tcccgcacat gggctggaac gaggtcgtca
     5041 ccgccgatgc gatcatggac ggcatcgagg acaggcactt ctacttcgcc cattccttcc
     5101 gcgggcgccc caccgacggc tccgccgtgg gccataccga ccacttcggg agcttcccct
     5161 cgctcttccg caagcggaac gtctacggga cgcagttcca ccccgagaag agctcgtcgg
     5221 ccgggctggc cttcctcagg aacttcgtgc ggttcgcgga ggggtgcgca tgatcgtcgt
     5281 acccgcggtg gacatcctgg accaccgggt ggtccagctg gtaggggggg tcccggggac
     5341 ggagcgggtg gtgctccccg accctgtcgc ggtggccgag ggttgggagg ccgagggggc
     5401 caggaggctg cacgtggtcg acctcgactc cgccctgggg aaggggcaca acgcccaggc
     5461 gatcagacgg atcgtgcagg gggccggcat accagtccag gtgggcggag gcatccggtc
     5521 ctccgaggtg gtggagtact acctcgactg cggggcggac agggtcatcg tggggaccaa
     5581 ggggctgaag gaccccgcct ggctgcgaag gatggcggag aggcgcccgg ggaggcttgt
     5641 gctggccctc gacctgaggg gcgggcgggt ccaggcccag ggctggaggg aggggctgcc
     5701 cctgggattg gaggacgtct tcgccacgat cgccgacatg ccgctcgccg gggtgctcta
     5761 caccaacgtg gatgtggagg ggcagggcgc gggcatcgac gccggggcgg tgcgcgcctt
     5821 catggccgcc tgcccgcacc cgaccatagt gtccggcggg atcaccaccg agcgggacct
     5881 ggaggcgctc gagggcatgg gcgtccggga ggcggtggtg ggattagcgc tttatactgg
     5941 gaagctgaat cacagcgtga tccggagatg aggccatgga ccgcaagacc gaggtagtgc
     6001 gcaagacccg ggagaccgag gtccggttga agctggacct ggacggggag gggaatgccg
     6061 atgtgcggtg cgaggagcag ttcctgcgcc atatggtcgg gaccctggcc cggtacgccg
     6121 acttcgatct ggccctggag gccaaggggg acgacgtgca ccacctggtc gaggacgtgg
     6181 cgatcgtcct ggggaagacg ctcaagcagt gcctgggcga atccccgatc gagcgcaccg
     6241 ccttctccct catcccgatg gacgatgcgc tggtgcaggt ggcggtggac atcatcgagc
     6301 ggccatacgc ggacgtggac tgccccaacg ccctgtacgc ccacttcctc cgcagcttcg
     6361 ccatgtcctc ggggataacg ctgcacgtca tggtgctccg cgggtacgac gagcaccaca
     6421 tcgtggaggc ctcgttcaag gccttgggca aggcgctgcg cgccgcggtc cgcccgcgca
     6481 ggagcgagct cagcaccaag gaccgcccca aggtgagcac ggaatgagcc tgaccaagag
     6541 gatcatcccc tgcctggacg tcaaggacgg ggcggtcgtc aagggcgtcc ggttcctgga
     6601 cctgaaggac gtgggctcgc cgccggcgat ggccgaggag tacgaacggc agggggcgga
     6661 cgagatcgtg ttcctggaca tctccgcgag cgtcgagggc aggcggacgc tgctcggcgt
     6721 ggtggaggag acggccaagc ggctgttcgt gcccctgacc gtgggcgggg gcatccggga
     6781 gacagcggac gtgcgggcgg ccctcaacgc cggggcggac aaggtgtcga tcaacaccgt
     6841 ggcggtgcag cggccggaga tcatctccga gtgcgccaag gacttcggct cccagtgcgt
     6901 ggtggtcgcc atcgacgcca agcgccgcgc gggcgggtgg acggtgtaca cgcacggggg
     6961 gcggagggac gcccagctgg acatggtgcg gtgggccaag cgggtggagg aactgggggc
     7021 cggggagatc ctgctgacga gcatggacgc cgacggcacc aaggacgggt acgacatcgc
     7081 gatgaccagg gcggtggcgg acgcggtctc catcccggtg atcgcctcgg ggggatgcgg
     7141 ctcgccgagg cacatcctgg aggtgctgag ggagaccaag gcggaggcgg ccctcgcggc
     7201 gtccatcttc cactacgggg agtggacggt cggggacgtc aaggaatatc tgaggaggaa
     7261 cggggttgag gtgagatgag cgcccccaag ttcgatgccg atgggctcgt ccctgtcatc
     7321 gtgcaggacc acgagaccaa cgaggtgctg atgatggcct atgccaacga gcaggcgtac
     7381 cgggacatgc tgcggaccgg ctacacccat ttctactccc gttcccggca ggcccagtgg
     7441 aggaaggggg agacgtctgg gcatgtccag gagatcgtct ccatccagct cgactgcgac
     7501 ggcgacgcgc tcctcgtgcg ggtgaggcag acgggggtgg cctgccacct cgggcggatg
     7561 tcgtgcttcg acgaggtgct ctacggggac cccgatggca ccatggccgt gctccccccg
     7621 ctcctgcggg tcatccggga gcgcagggcc gagccccggg agggcagcta cacgtgcgcg
     7681 ctcttcgcgg acgagaacaa gcgcctgaag aaggtggtcg aggaggcggg ggaggtgctc
     7741 ctggcggcca aggaccggaa ggccgaggag caggcctggg aggtggccga cctcgtgtac
     7801 cacctcctgg tggcggtgga ggggatggaa gtgacctggg aggacgtcta ccggaagctg
     7861 gcggagagga ggagatgagg gcggagctgc acacccacac cctgctgagc gacggcgagc
     7921 tgctgccgat cgagctggcc cgccgcgcgg tggtgaaggg gacgaagtac ctggcgttca
     7981 cggaccacgt gtcgctcagc aacctggagc gggtcgtggc cgagggccgg cgggacgccg
     8041 agctggcgga ggaatggggc atgaaggtgc tggccggggt ggaggtcacg cacgtccccg
     8101 tccggcgcat cgacgaggtc gtgctcaagg cgcgcaggct cggcgcggag ctcgtggtgg
     8161 tccacgggga gacgctcagc gagccggtgg agaaggggac gaacatggcc gccgtgctca
     8221 accccgaggt ggacatcctg gcccatccgg ggctcatcac cctggaggag gcgcagctgg
     8281 cccgcgacaa cggggtggcg ctggagatca cctcccggag ctcccactgc aaggccaacg
     8341 ggcatgtggc ccggatggcc cagcaggccg gggcgcccat ggtcgtgaac acggacgcac
     8401 acggccccga cgacctcatc gatctcccca cggccctgca gctcgcgctg gcggccgggc
     8461 tgacccgcga ggaggcggac cgggcgctca tcgaggtgcc gaaggccatc atcaagcgga
     8521 ggtggcgcgg ctgaaggtgc tcaagttcgg cggctcctcg ctgcggaccg gggggtccat
     8581 ccggcaggtc gccgggatca tcatggccga gcgggagaag aaggccgtgg tgctgagcgc
     8641 ggtgacgggc gtcaccgaga tgctggtgca gttcatctcc cgcacccgga gcgaggagga
     8701 cgtggacgcc ttcatgcggg agatcaccaa gctgcacgtc gatctcgcgc gggacgccct
     8761 gcgcaccgag agcgcccggg ccaaggccgt ggaggagctc gtggccaagc tggtgaagct
     8821 ggagcgcatg ctctacggct cctgctacat agaggaggtc aacccccgca tccgcgacta
     8881 cgtgcagtgc ttcggggaac ggctctccgt cataatcctc tcggccctgc tgaacgagct
     8941 gggggagagg gcgcggccgg tggacgcgga cgagctgggc atcatcaccg acggcctgta
     9001 cggggcggcg acggtcaaca tggaggcgac gcgcgccagc atccgcccga agctgctgcg
     9061 gatgctgcag gaggacacca cgccggtcat caccggcttc ttcgggcggg ccccggaggg
     9121 ctccgtcacc gtcttcgggc gcaacgggag cgactacagc gcggcggcgg tggccaacgc
     9181 catgggggcg tcctccctgg agatctggaa ggacgtggac gggttcatga gcgtcgaccc
     9241 caagatcgtg ccctcggcca agcccatccc ccgcctcagc tacgacgagg cggccgagct
     9301 cgcgtacttc ggggccaagg tcgtgcatcc catgaccatg gagccggccc gctcgggcaa
     9361 catcgagatc cgcgtcaaga acgtgttccg gccggaggcg cccggcaccg ccatcggcgg
     9421 ggccgacaac gtcgatcaga gcatcaagtc gatctcctgc atgaaggacc tctccctggt
     9481 gaaggttttc ggggagggca ccgggtacaa gctcggggtg ctggccgacg tcgcctcgct
     9541 gctgcggagg gcggagatca acatctacag cgtggtcacg tcgctgacct gcatcgcgct
     9601 gctcatcgac cggagggacg tggccaaggc ccagaaggtg ctggcggagt cgggcgcctc
     9661 gagggtggag acgcacaccg gcttcgccct catgtgcatc gtcggcgagg ggctggggca
     9721 ccacaagggg ctggcctccc gcgtcttcaa ggcggtggcg aaggaggggg tgaacgtcgg
     9781 actgatctcg gccggcgcct cgctggtcgc ctaccacttc acggtggacg aggcggacct
     9841 ggagcgggcg acgagggcgg tccacgagga gctgttcggg tgctgagatg atcatagcgt
     9901 tccagggggt gaggggggcg tacagcgagg acgcggtgcg cgcctgggac gagggggccg
     9961 aggccctgga gtgtcccgag ttcatcgacc tcttcgacgc ggtgcgctcc ggcagggcgg
    10021 acctggggat gctgccggtg gagaactcca tcgaagggag cgtgacggcc gccaacgacc
    10081 tcctgttccg aagcgacctg tgggtggtcg gggaggtcat ggtgcgcatc aggcactgcc
    10141 tcatcggcca tcccggcgcc ggggtccagg acgtgcggcg ggtgtacagc cacccccagg
    10201 cgctggccca gtgccgcgac ctgctgacct cgcacccgga gtgggagaag gtccccgcct
    10261 acgacaccgc cggctcggtg cggatggtca aggagcgggg gctgcgggag gaggcggcga
    10321 tcgccagccg gcgcgccgcc gagacgtacg gcatggaggt gctcatggag gacgtgcaga
    10381 gcgtccggga caacatcacc cgcttcttcg tcctctccag ggagcggggg acggggaggg
    10441 gggacaagac cgcccttgcg ttcaccacca agaaccgccc gggggcgctg ttcgattgcc
    10501 tgcgggcgtt cgcggacaac ggcgtcaaca taaccaagct cgagtcccgg ccctggcgcg
    10561 gccgggcctg ggagtacgtg ttctatctgg acctggacgg cgacgtggag gacgagaggg
    10621 tcgccgcggc gctccgggcg ctgcgggaga gcgcctcgtg cgtgaaggtc ttcggc
//
LOCUS       DAOSYN010000025        10528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_5372_length_10528_cov_4.212833, whole genome shotgun sequence.
ACCESSION   DAOSYN010000025 DAOSYN010000000
VERSION     DAOSYN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5372_length_10528_cov_4.212833"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLJ11_02110"
     CDS             complement(<1..255)
                     /locus_tag="PLJ11_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOO03495.1"
                     /translation="MDVRLLSWNVNGIRAAQRKGLADLLASDGADVVCLQETKAAPEQ
                     LPTELREVPGYRSYFASPKDRKGYSGVAMYTKVEPRRVVPG"
     gene            346..762
                     /locus_tag="PLJ11_02115"
     CDS             346..762
                     /locus_tag="PLJ11_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO03496.1"
                     /translation="MTSFKRILVGVDGSENALRAAGFAAALAKGFGAEITLAHVIAPR
                     DHDLLVGKATYADKDFKVGGERLKGAERLLEQEGVPFRSTVEFGHPAEQLLRLAEGQD
                     LIVVGTRGLSPFKGILIGSTSTRLAQGSKVPVTIVP"
     gene            919..2202
                     /locus_tag="PLJ11_02120"
     CDS             919..2202
                     /locus_tag="PLJ11_02120"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO03497.1"
                     /translation="MDGSGVGTGSWKRGFFTIWGGQAFSLVGSGLVQFALVWWLTVET
                     GSATVLALGTLMGVLPQIVIAPWAGAYVDRWNRRLTMIGADSLIALLTVLLMLAFLWG
                     AAEVWMLLLVMFGRATLAGFHWPAMQAATSMMVPEQHLGRVGGLNQAMYGLSNVIAAP
                     AGAVLIALFPMWAVLSVDLVTALMAIAPLLVIPVPEPDRKGAPKPSLLREMREGLAFL
                     RGWKGALLVIVLYMVANLLLSPAFSLLPLLVIDHFQGGAGEYATMEALAGIGMLVGGM
                     ALGVWGGSRRRTRTMLASTLALSLAITALGIVPAELLPLAYVLSGAIGATLSLVNGSM
                     MAMMQASVPFAIQGRVFALISAGVTAMMPVGLLIAGPLGDALGVRAWYLIAGIPMTLL
                     TLVYMASPTVVHLEDQVMDGSAKDAKERDERSPGA"
     gene            2234..3160
                     /locus_tag="PLJ11_02125"
     CDS             2234..3160
                     /locus_tag="PLJ11_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03498.1"
                     /translation="MSGPDPLHDYLSILQGKARPRFQELKGGPTSELSLKVAQAERLE
                     AGCALCEHRCGANRREGRPGRCGVLRTKVASHFIHYGEERPLVPSYTVFLAGCNLRCV
                     FCQNWDISTDAEAGEVIEPGVLARMIEARGIAPIGAPACRGIRNVNWVGGDPIPHLPY
                     VLRVLQELELDVPQVWNSNMYMTREALEVLAGTMDVYLTDLKFGNDRCARKLCGAPRY
                     WEVVTRNHLQAARQGEVVVRHLMLPGHLECCTLPVLEWLGEHLSQAAVNVMDQYRPEH
                     RARQMPEMARGVSPTEHRVALERAGELGLLLI"
     gene            3233..4906
                     /locus_tag="PLJ11_02130"
     CDS             3233..4906
                     /locus_tag="PLJ11_02130"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOO03499.1"
                     /translation="MDYYAIAVLGPNVLAAAIIAWVAYRVLTGKKTEHRLAMGICFAF
                     FSLTCLVLLVEKTVGDMALARAVVLLEYICESVVLVALLMFVLQYIGLGRYANRRNAL
                     LSALPAMVTIGLYVTNDLHHLFYNDVAIVPSHGFYMFDADYGPLFLIWAGYFLGVFLI
                     TALLMVRALLETPSYRRGGLRTMTLAIGTMALISVLYVLSPRDDPRIDILSIGFTIAA
                     LIVFFGERYSDFADLEIIRFREAIGGMDDAVIILGPSMEVVYANGPGRRLLEQNAEFF
                     WERVKARGLRVPSGPNRWETAMVIDGEPRHFILSTSDILRQGKPIGMVMVFHDISNRK
                     GLEEELRRANRSIRLLNEVLRHDLMNDMTALWGYMELLEATELNDRQRHLVGKLIERA
                     RSAEAQLSFAAEQERNLATTTAWHDMQAIITKVLGQVDMNGIRTESHLEGLQVLADPK
                     LENVFHVLADNTVRHGRKATRAVVRAENTETGLEIIWEDDGVGVPAESKKNIFSKGVG
                     DNTGLGLYLAREILATTGFTISEEGEPGKGARFVIRVPSGWFTWKMHPS"
     gene            complement(4911..5198)
                     /gene="hgcB"
                     /locus_tag="PLJ11_02135"
     CDS             complement(4911..5198)
                     /gene="hgcB"
                     /locus_tag="PLJ11_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HOO03500.1"
                     /translation="MSPQNGGRINTLVFYPGRCVRCGMCLKVCPHRVFALEGERIRLA
                     RPADCMECGACRKNCPSHAIEVDSGVGCASAMIKAALRGREDVVCGEDCCR"
     gene            complement(5222..6202)
                     /gene="hgcA"
                     /locus_tag="PLJ11_02140"
     CDS             complement(5222..6202)
                     /gene="hgcA"
                     /locus_tag="PLJ11_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HOO03501.1"
                     /translation="MEEGYSQEVMRTTATLGRKEAWDHAKARMGIARMGHRVRPGLYA
                     LGEPTRSSPVLVTANYSLSFDALRSSLPGRDAWVLVLDTGGINVWCAAGKGTFGTEEL
                     VRAIGATKLSERVDGRTLILPQLGAPGVSAQEVHGATGFKVEYGPVRAEDLPFYLDRG
                     ECTDAMRTVRFDLRDRLVLAPVELRNHRWGLALLVLLAVLVPFYGLEALVVALGGLLL
                     FPALLPYLPGKDLSLRGMALGALLVTPFALFQLASGMDAATLLAVPAEFLLMVPLAGY
                     LGLNFTGSTPFTSRTGVRREIFTYIPKMAAMAVLGAVIGGVALLVHWGWL"
     gene            complement(6207..6476)
                     /locus_tag="PLJ11_02145"
     CDS             complement(6207..6476)
                     /locus_tag="PLJ11_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HOO03502.1"
                     /translation="MPRRKEIAGEECCSAPAMPNKYDVEGVATVDERGQMVLPKAVRE
                     KAGIRPGDRLAIVTMVRDGRVCCLHLFRTDDLAATARDILERRED"
     gene            6569..7081
                     /locus_tag="PLJ11_02150"
     CDS             6569..7081
                     /locus_tag="PLJ11_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO03503.1"
                     /translation="MRAAGREVEIVQASPGNVEALLSLITELAEFEGLEPPTEEARER
                     LRHHITVSPAPFHAFIAYSDGRAVGYITYYFTYSTFLAEPTLFLEDIYVREKERMQGI
                     GGKLFRHCVREAIAKGCGRMEWCALNWNVDAMAFYERQGARRMDWTFFRLDRKAMERL
                     EAKEGAARLV"
     gene            complement(7091..8278)
                     /locus_tag="PLJ11_02155"
     CDS             complement(7091..8278)
                     /locus_tag="PLJ11_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOO03504.1"
                     /translation="MTEQVPKIAIYLDFENLAISAKEVYPLTPKPLRIDDILDYAKGY
                     GDVLIKKAFADWNKPPCSQYVEDLRLRAFDLVFLPQTSLSGKNGADLRMTIDALEDMY
                     NKSLLDVFFLGTGDTDFIHLIRKIKERGKEVVVLGFEHSIGRTIRYNCDRFESLEDLL
                     GKAPAPPGVEKKEEEELEDDPRELFLRYIKNRSTDEPAILSQIKNDLMRLDPSFSEKK
                     YGYKHFKEFLDSFKGDLFTTIKMDDKAGHHLVFFKSYAELTSDINEAQVRDFIDKEMR
                     FIRDRALRKALYKETIGLFHADGETTINQMVDDLWALFDKKVPKATLKRFLRTMAEGR
                     MFKFANAKYTGNIYTMPQVLKTDLPSLERLDEIYLQRIIDLTLRKCPGVPQESVTRMM
                     GLS"
     gene            complement(8321..9154)
                     /locus_tag="PLJ11_02160"
     CDS             complement(8321..9154)
                     /locus_tag="PLJ11_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO03505.1"
                     /translation="MVKGHGASDVMLRLNNGVEVPALGLGTYKLVGEAAYAPVRAALG
                     CGYRHIDTASFYENEEAVGRAVRESGVPREEVFITSKVWNTEQGFEETLNAFDRTLQR
                     LGMDYLDLYLVHWPVPGKRLETYRALERLYEEGRVRAIGVSNFTVRHLEELYGACQVM
                     PAVNQVEMSPFLYQKELLEHCRGRNVLVTAFSPLARGQALGDPVLAEVAARHRRSPAQ
                     VMIRWCLQKGMAVIPRSADPAHIKENADVFDFSLDEEDMARLDLLHTGLRTTTDPDIY
                     E"
     gene            9432..10214
                     /locus_tag="PLJ11_02165"
     CDS             9432..10214
                     /locus_tag="PLJ11_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03506.1"
                     /translation="MRIMEWNLQYGGAQERLPGILEAVRRHAPDLLVLLEFRPERVIE
                     VSLSLTALGYPYILNSQPPPRTSGILVASKGALVQMEAPRVRSSPHRWMEVSPEGSDL
                     RILAVHVPGASDLPGKMEFWQTLLEYGKEAVERKDRRVIIGDLSTGLERDSEGTPFLG
                     TEHLAALLGMGWRDVWREYHQVAREYSRYSSSGKGMRTDHALASPHIHHPLWAKYSHQ
                     ERESGISDHSILILDIMHKLSESGSRSSPLFRLDEGPGCIMG"
     gene            10307..>10528
                     /locus_tag="PLJ11_02170"
     CDS             10307..>10528
                     /locus_tag="PLJ11_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HOO03507.1"
                     /translation="MDGSISKKLVAEMIGTFVLVFGGVGTAVLAGAWVGALGVALAFG
                     FTLLVMAYAIGPISGCHINPAVTLGMFMAK"
BASE COUNT         1904 a         3266 c         3374 g         1984 t
ORIGIN      
        1 gccagggacg acccttctcg gttcgacctt cgtgtacatg gccaccccgc tgtagccctt
       61 ccggtccttg ggggaggcga agtaggagcg gtagccgggg acctccctga gctccgtcgg
      121 cagctgctcg ggggcggcct tggtctcctg caggcatacg acgtccgcgc cgtcgcttgc
      181 cagcaggtct gccagcccct tcctctgggc cgccctgatc ccgttcacgt tccagctgag
      241 caagcgcaca tccatcgccc tgctggggat gggggctcca ggataataaa gggtcgtgga
      301 aaacctttag gcctttgaca ggcctaatat atcatggtga cagcgatgac ctctttcaag
      361 cggatactgg tcggcgtcga cggctcggag aacgcccttc gagcggcggg cttcgcggcc
      421 gcgttggcca agggcttcgg ggccgagatc acgctggcgc atgtgatcgc tcccagggac
      481 catgacctcc ttgtggggaa ggccacctat gcggacaagg acttcaaggt cggaggggaa
      541 aggctgaagg gggccgagag gctcctggag caggagggcg tgccgttcag gtccacagtg
      601 gagttcggcc atccggccga gcagctcctg cgcctggccg aggggcaaga cctgatcgtg
      661 gtcggtaccc gcgggctcag ccctttcaag gggatcctga tcggcagcac ctccacccgg
      721 ctggcgcagg ggagcaaggt cccggtgacg atcgttcctt gagctcgagg ccgatcagcc
      781 tcagccctcc ccgttcatgc aaaggttctt ttctggctaa gggatacgga agcggcggcg
      841 ggggagcggt gggggaaggc cgagggcggt cattcctgcc ctttcgatca aagggcgggg
      901 tccaccgccg attgtcatat ggacggaagc ggggtaggaa cgggctcatg gaaaaggggc
      961 ttcttcacca tctggggagg gcaggcgttc tccctggtgg gcagcgggct tgtccagttc
     1021 gcccttgtct ggtggctcac cgtggagacg ggatcggcca cggtccttgc cctgggaacg
     1081 ttgatggggg tgctcccgca gatcgtcatc gctccctggg ccggggccta cgtcgaccgc
     1141 tggaacagac ggctgacgat gatcggggcc gattccctca tagcgctgct gaccgtcctg
     1201 ctgatgctgg cattcctgtg gggggccgcc gaggtgtgga tgttgctgct cgtgatgttc
     1261 ggccgggcca ccctcgccgg cttccactgg ccggccatgc aggccgccac ctccatgatg
     1321 gtccccgagc agcacctggg gagggtgggc ggcctcaatc aggccatgta cgggctgtcc
     1381 aacgtcatcg ccgccccggc gggggcggtg ctcatcgccc ttttcccgat gtgggccgta
     1441 ctgtcggtgg acctcgtcac cgcgctcatg gccatagccc ccctgctcgt catccctgtg
     1501 cccgagcccg ataggaaggg cgctcccaaa ccatcgctgt tgagggagat gcgggagggg
     1561 ctggccttcc tgaggggatg gaagggggcg ttgctggtca tcgtcctcta catggtcgcc
     1621 aatcttttgc tgagccccgc gttctcgctg ctgcccctcc ttgtcatcga ccatttccag
     1681 ggaggggccg gggaatacgc cacaatggag gccctggccg gcatagggat gctggtcggc
     1741 ggcatggcct tgggggtctg gggaggctcc aggcgcagga cccggaccat gctcgcctcc
     1801 accctggccc tcagcctggc cataaccgcg ctgggcattg ttcccgcgga acttcttccc
     1861 ctcgcctacg tgctgtcggg ggcgatcggc gccaccctct ccctggtcaa cggctccatg
     1921 atggccatga tgcaggcgtc ggtaccgttc gcaatccaag ggagggtgtt cgcgctcatc
     1981 agcgccgggg tcacggcgat gatgcccgtc ggcctgctga tagcggggcc gctgggggac
     2041 gccctcggcg tgcgcgcatg gtacctgatc gccgggatcc caatgacttt gctcaccctg
     2101 gtgtacatgg cctcaccgac ggtggtccat ttggaggacc aggtcatgga cggctcggcc
     2161 aaggacgcca aggagaggga cgagcgctcg cccggggcat gattcattag gggcgaaggc
     2221 gcataatgca ccgatgagcg gcccggaccc cctgcacgat tacctctcca tacttcaagg
     2281 gaaggccagg ccgcggttcc aagagctgaa ggggggaccg acctccgagc tcagcttgaa
     2341 ggttgcgcag gctgagaggc tcgaggcggg gtgcgcgctg tgcgagcatc gctgcggcgc
     2401 gaaccgcagg gaagggcgtc cgggaaggtg cggggtcctg aggacgaagg tggcctcgca
     2461 cttcatccac tacggggagg agaggccgct ggtgccctcc tacaccgtct tcctggccgg
     2521 ctgcaacctg cgctgcgtct tctgccagaa ttgggacata agcacggatg ccgaggccgg
     2581 agaggtgatc gagccgggcg tgctggccag gatgatagag gcgcggggga tcgcccccat
     2641 cggcgccccc gcctgtcggg gcatccgcaa cgtcaactgg gtcggcgggg accccatccc
     2701 gcacctcccc tacgtgctga gggtccttca ggagcttgag ctggacgtgc cccaggtatg
     2761 gaactccaac atgtacatga cccgcgaggc cctcgaggtg ctggccggga ccatggacgt
     2821 ctatctgacc gatctcaagt tcgggaacga caggtgcgcc cggaagctgt gcggcgcccc
     2881 ccgctactgg gaggtcgtca cccgcaacca tctgcaggcg gccaggcagg gggaggtcgt
     2941 ggtcaggcac ctgatgctcc ccgggcacct ggaatgctgc accctccccg tcctggaatg
     3001 gctgggcgag catctttccc aggcggcggt gaacgtaatg gaccagtacc gtcccgagca
     3061 ccgggcccgc cagatgcctg aaatggcgcg gggcgtttcc ccgacggagc accgcgtcgc
     3121 cttggagagg gcgggggagc taggcctcct gctcatctga tgtcagacat tggctgggtt
     3181 acgccacatt gcttatatcg cttctaagcc catccgcgtt gggtagggga acatggacta
     3241 ctatgccatc gccgtcctag ggcctaacgt tctagcggcc gccatcatcg catgggtagc
     3301 atacagggtc ctcaccggaa agaagaccga gcaccgcctg gccatgggca tatgttttgc
     3361 cttcttctcc ctgacctgcc tagtgctgtt ggtggaaaag acagtcgggg acatggcctt
     3421 ggcgcgggcg gtggtcttgc tcgagtacat atgcgaatcc gtggtcctcg tcgccctgct
     3481 tatgttcgtg ctccaataca ttggactggg caggtacgcg aaccgccgca atgcgctcct
     3541 gagcgccctc cccgccatgg tgaccatcgg actgtatgtt accaacgacc tgcaccacct
     3601 cttctataac gatgttgcga tcgtcccgag ccacggcttc tacatgttcg atgccgacta
     3661 cggccccctg ttcctcatct gggcgggcta cttccttggg gttttcctga tcacggccct
     3721 tcttatggtc agggctctcc ttgagacccc ctcctaccgc agaggggggc tcaggacaat
     3781 gaccctggca attggtacca tggcactcat aagcgtcctc tacgtcctga gccccaggga
     3841 cgacccgcgg atcgacatcc tctcgatcgg cttcaccatc gccgccctga tcgtcttctt
     3901 cggtgaacgg tactcggact tcgccgattt agagatcatc cgtttcaggg aggccatcgg
     3961 aggaatggac gacgccgtga tcatcctggg cccttcaatg gaggtcgtgt acgccaatgg
     4021 gcccgggcgg aggttgttgg agcagaacgc cgaattcttt tgggagaggg tgaaggccag
     4081 ggggctgagg gtgccctccg gtcccaaccg ttgggagacc gcgatggtca tcgacggcga
     4141 gcccaggcat ttcatcctct ccacctcgga catcctcagg cagggcaagc ccatcgggat
     4201 ggtgatggtg ttccatgaca tcagcaaccg gaagggcttg gaggaggagc tgcgaagggc
     4261 gaaccggagc ataaggctcc tgaatgaggt ccttcggcat gacctgatga acgacatgac
     4321 cgccctatgg gggtatatgg aactcctcga ggccaccgag ctgaacgacc gacagcggca
     4381 cctcgtcgga aagctgatcg agcgcgccag gagcgccgag gcgcagctca gctttgcggc
     4441 ggagcaggaa cggaacctag cgacgaccac ggcctggcat gatatgcagg cgatcataac
     4501 gaaagtgctg gggcaggtgg atatgaacgg catcaggacc gagtcccacc tggaaggtct
     4561 ccaggtgttg gcagatccca agctggagaa cgtcttccat gtcctggccg acaacaccgt
     4621 gcggcatgga aggaaggcca cccgggcagt ggtccgcgcg gagaacaccg agaccggcct
     4681 agagataatc tgggaggatg acggagtggg cgtcccggcg gagagcaaga aaaacatctt
     4741 ctccaaaggg gtcggggaca acaccggcct cgggctctac ctggcgcggg agatcctggc
     4801 cacgaccggg ttcacgatct ccgaggaagg ggagcctggg aagggggcgc ggttcgtaat
     4861 aagggtgcca tccggctggt tcacctggaa gatgcaccct tcatgaccca tcagcggcag
     4921 cagtcctcgc cgcacaccac atcctccctt cccctcagcg cggccttgat catggcggag
     4981 gcgcacccca ccccgctgtc cacctcgatg gcgtgggagg ggcagttctt ccggcaggcc
     5041 ccgcactcca tgcagtcggc cggcctggcc aatctgatcc gttccccttc gagggcgaag
     5101 acccgatggg ggcacacctt caggcacatc ccgcaccgga cgcatcggcc gggatagaat
     5161 accagggtgt tgatcctccc accattttga ggggacatga catcgagcgt tccgatctcc
     5221 atcacagcca cccccaatgg acgagcagag ccacccctcc gatcactgcg cccagcaccg
     5281 ccatcgccgc catcttgggg atataggtga atatctccct gcggaccccg gtgcgggagg
     5341 tgaagggggt ggagccggtg aaattgagcc ccaaatagcc ggccaacggg accatcagca
     5401 ggaattcggc gggaacggcc aaaagcgtcg ccgcatccat accggacgcc agctggaaca
     5461 gggcgaacgg ggtcaccaag agcgccccta gagccatccc ccgcagggac aggtccttcc
     5521 cgggaaggta gggaaggagg gcggggaaca ggagcagccc tccaagggcc accaccaggg
     5581 cctcgagccc gtagaagggc accaagaccg ccaacagcac caggagcgcg agcccccatc
     5641 ggtggttcct cagctcgacg ggggccagga cgaggcggtc gcgcaagtcg aagcgcacgg
     5701 tccgcatcgc gtcggtgcac tcccctcggt ccagatagaa cggcaggtcc tccgcccgga
     5761 ccggtccgta ctccaccttg aacccggtgg ccccatggac ctcctgggcc gacactcccg
     5821 gcgctcccag ttgggggagg atgagggtcc ggccgtccac cctctcggag agcttggtgg
     5881 ccccgatcgc ccggacaagc tcctccgtgc cgaaggtccc cttgccagcc gcgcaccata
     5941 cattgattcc cccggtgtca agcaccagca cccaggcgtc cctgcccggg agggaggagc
     6001 gcagggcgtc gaagctcagg gaatagttgg cggtcacgag gaccggcgag gagcgggtcg
     6061 gttcgcccag ggcatacagc ccggggcgga cgcgatgccc catgcgggcg atccccatcc
     6121 gggccttggc atggtcccag gcctccttcc tccccagggt ggccgtggtc ctcatgacct
     6181 cctgcgaata cccttcctcc acgggctcag tcctccctgc gttccaggat gtccctcgcg
     6241 gtcgcggcca ggtcatccgt tctgaacaga tggaggcagc acactcgccc gtcccgtacc
     6301 atggtgacta tggccagcct gtcccccggc ctgatgccag ccttctccct cacggccttg
     6361 ggcagcacca tctgcccgcg ctcgtccacg gtggccaccc cttccacatc gtatttattg
     6421 ggcatagctg gcgcggagca gcattcctct cctgcgatct ccttacggcg gggcatgcgc
     6481 ctccatcggt atctatctat ttgaaatttc tgaaaaatca gaaaatgctt aagcatgaag
     6541 aaataggcgc acaacgctat cggagagcat gcgcgcagct ggaagggagg tggagatcgt
     6601 ccaggcgtcc ccggggaacg tcgaggcgct gctgtccctc atcacggagc tggccgagtt
     6661 cgaggggctg gaaccgccga ccgaggaggc gagggagcgg ctgcggcacc acatcaccgt
     6721 ctcccccgct ccattccacg cgttcatcgc ctattcggat ggaagggcgg tggggtacat
     6781 cacctactac ttcacctatt cgaccttcct cgccgagcct accctgttcc tcgaggacat
     6841 ctatgtcagg gagaaggagc gcatgcaggg gatcggaggg aagctgttca ggcattgcgt
     6901 cagggaggcc atcgccaagg ggtgcgggcg catggaatgg tgcgccttga actggaacgt
     6961 tgacgccatg gcgttctacg agaggcaggg cgcccgaagg atggactgga ccttcttccg
     7021 cctggaccgg aaggcgatgg agcgcctgga ggccaaggag ggggcggccc gcctggtcta
     7081 gcctgagccc ctagctcagc cccatcatcc tggtcactga ctcctggggc acgcccgggc
     7141 acttcctcaa cgtcaggtcg atgatgcgct gcaggtagat ctcgtccaac cgctcgagcg
     7201 atggaaggtc ggtcttcagc acctgcggca tggtatagat gttgcccgta tacttcgcgt
     7261 tggcgaactt gaacatgcgc ccctcggcca tggtccgcag gaagcgcttc agcgtggcct
     7321 tcggcacctt cttgtcgaac agggcccaga ggtcgtcgac catctgattg atggtcgtct
     7381 ccccgtccgc atggaagagg ccgatggtct ccttgtagag cgccttgcgc agggcgcggt
     7441 cccgaatgaa gcgcatctcc ttgtcgatga agtcccggac ctgggcctcg ttgatgtccg
     7501 aggtgagttc ggcgtagctc ttgaagaaca caaggtgatg gccggccttg tcgtccatct
     7561 tgatggtggt gaagaggtcc cccttgaagg agtccaggaa ctccttgaaa tgcttgtatc
     7621 cgtacttctt ctccgagaag gaggggtcga gccgcatcag gtcgttcttg atctgggaga
     7681 ggatcgccgg ctcgtccgtc gagcggttct tgatgtaccg gaggaacagc tcgcgcgggt
     7741 cgtcctccag ttcctcctcc tccttcttct ctacgccagg gggggctggg gccttgccca
     7801 acaggtcctc cagcgattcg aagcggtcgc agttgtagcg gatggtccga ccgatggagt
     7861 gctcaaagcc cagcaccacg acctccttcc cgcgctcctt tatcttccgt attaggtgga
     7921 tgaagtccgt gtccccggtg ccgaggaaga acacatcaag cagggacttg ttgtacatat
     7981 cctctagggc gtcgatggtc atgcgcaggt cggccccgtt cttgcctgaa agggaggtct
     8041 gcggaaggaa caccaggtcg aaggcccgca gccggaggtc ctcgacatac tggctgcatg
     8101 gcggcttgtt ccagtcggcg aacgccttct tgatgagcac atccccatat cccttggcgt
     8161 agtcgaggat gtcatcgatc ctcaggggtt ttggggtcaa agggtagacc tccttggcgg
     8221 aaatggccag gttctcgaag tctaggtata tcgctatctt agggacctgt tcggtcatct
     8281 atccaccatc cgctcctcca tgacttaatc ctttcctgcc ctattcgtag atgtccgggt
     8341 cggtcgtggt gcgcaatccc gtgtgcagaa ggtccaaacg ggccatgtcc tcctcgtcca
     8401 aggaaaagtc gaacacatcg gcgttctcct tgatgtgcgc ggggtccgca gacctgggga
     8461 tgacggccat gcccttctgc aggcaccacc ggatcatcac ctgcgccggg ctcctgcgat
     8521 gccgcgccgc cacctcggcc agcaccgggt ccccaagggc ctgcccgcgg gcgagggggg
     8581 agaacgcggt gaccagcacg ttccttcccc ggcaatgctc caggagttcc ttctgataga
     8641 gaaaggggct catctccacc tggttcaccg cgggcatgac ctggcatgcc ccatagagct
     8701 cctccagatg acggaccgtg aagttgctga ccccgatggc cctaacccgc ccctcttcat
     8761 ataggcgctc gagcgcccgg taggtctcca agcgcttccc tgggaccggc caatgcacca
     8821 ggtagaggtc caagtagtcc atgcccagcc gttgcaaggt tcggtcgaag gcgttcaggg
     8881 tctcctcgaa tccctgctcg gtgttccaga ccttgctggt gatgaagact tcctcgcggg
     8941 gcaccccgct ctcccgcaca gcccttccca cggcctcctc gttctcatag aacgaggcgg
     9001 tgtcgatgtg acggtatccg catcccaggg cggcgcgaac gggggcatag gccgcttcgc
     9061 cgaccagctt gtatgtcccc aatcccaatg ccgggacctc cacgccgttg ttgaggcgga
     9121 gcatgacatc ggaagcgcca tgtcctttga ccatcagagg gggattcgga cggtcctagt
     9181 taatcgtatc ccatcgtttc ggtgataggg cctcggtccc ctgaacgtcc caaggaggat
     9241 tcgaatggcc gaaggggtgc tctgtgcaag gcggggaccg aagccgtagc acttgaaaat
     9301 agaatagtaa tactcagtat taaaagtaat tgtaaaatta ataccaacaa gccccgctac
     9361 ctcggagatg cgttttggcc attgcggacg ggggaaggtt catctaatgc atggtgcgat
     9421 agcaacggtc catgcgcatc atggaatgga atctgcagta cggaggggct caggagcgcc
     9481 tgccaggcat cctggaggcg gtccggcggc atgccccgga cctgctcgtc ctcctggaat
     9541 tccgtcctga aagggtcatc gaggtgtccc tctccctcac cgcgctcggc tatccataca
     9601 tactgaactc acagccgcct ccgcgcacca gcgggatatt ggtcgcctcg aagggcgcgc
     9661 tcgtgcagat ggaggcccct cgagtgcggt cctcgcccca ccgttggatg gaggtgtccc
     9721 ccgaagggtc ggacctgcgg atactggcgg tccatgtccc cggggcctct gacctcccgg
     9781 gaaaaatgga gttctggcag acgctcctgg agtacggcaa ggaggcggtc gagcgcaagg
     9841 accggagggt gatcatcggc gacctgagca cgggcttgga gcgggacagc gaaggcacgc
     9901 cgttccttgg cacggagcac ctggccgcgc tgctgggaat gggctggaga gacgtatgga
     9961 gggaatatca ccaggtggcc cgggaatact cccggtattc gtcctccggc aagggcatga
    10021 ggaccgatca cgccctggcg tcaccgcaca tccaccatcc tctgtgggcc aagtactcgc
    10081 accaggagcg ggagtctggg atatccgacc attccatctt gatcctggat ataatgcata
    10141 agctgagcga gtcaggttcc cggagctcgc cgctcttccg cctcgatgag ggaccgggct
    10201 gcatcatggg atgaaactct ggacgatagg gctttccgcc catctaaaat ccaaaaacat
    10261 taataatata taatccagaa gtctctgatt tagcgaggtt cagatcatgg acggatccat
    10321 ctcgaagaag ctcgttgcag agatgatcgg cacattcgtg ttggtcttcg gtggtgtggg
    10381 cacggcggta ctggccgggg cctgggtggg ggctttgggt gtcgcgctgg cgttcggctt
    10441 caccttgctg gtgatggctt acgccatcgg gccgatatct ggatgccaca tcaacccggc
    10501 cgtgacattg gggatgttca tggccaag
//
LOCUS       DAOSYN010000026        10340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_5485_length_10340_cov_4.465241, whole genome shotgun sequence.
ACCESSION   DAOSYN010000026 DAOSYN010000000
VERSION     DAOSYN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5485_length_10340_cov_4.465241"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLJ11_02175"
     CDS             <1..588
                     /locus_tag="PLJ11_02175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOO03508.1"
                     /translation="ILYIGLPLGAVYILKGEGAPYRRYILGGNFRIGRWEKLFLVLSS
                     AIFALAHVFSWDLYKVPPTFLAGLALGYLFLRYGVYASIMLHFTIDYLSLPMEVWPGA
                     GTELVMGLFVLVLNVVGAVYLVYYSVRAVELFSGRKLWNWGEERPVSAYYRPLSPKAQ
                     AVATPAFGFVCKHCANTEARYVDGRFVCTRCGRES"
     gene            complement(602..1366)
                     /locus_tag="PLJ11_02180"
     CDS             complement(602..1366)
                     /locus_tag="PLJ11_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOO03509.1"
                     /translation="MIAVSSPHLSHWEFPLAIAKVAEHYQAWEIVAEGRHSLAAIKPA
                     FLELSPSYDLEWSVHAPLSDVNIGALTPRMRQAALDEVLECLRLAHAMGIDLVTVHPG
                     LLSPLAALDREAAVRATLASLRAIDAAREDLGLRVALENMPEMPAALAKGPEELTRLI
                     EGTGLGVCFDIGHANTVGNIPAFLSIKDRFINMHVHDNCGDRDRHLVIGEGNIDLGRW
                     LRAFRPYGGRYVIEARELDRSLVSKGRLEAILASMK"
     gene            complement(1363..1743)
                     /locus_tag="PLJ11_02185"
     CDS             complement(1363..1743)
                     /locus_tag="PLJ11_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HOO03510.1"
                     /translation="MIVTAKARVFPTEDGEKVRRALLNIFPTAETAYDGDVLVATARD
                     LDRFREVLRNHRILDSARAVLLRGLSEDGLRFQLNKQAAFVGKVSFVEGGAPLGAIEV
                     LVADDALEALIDDVAPRTVDGEVP"
     gene            1799..3235
                     /locus_tag="PLJ11_02190"
     CDS             1799..3235
                     /locus_tag="PLJ11_02190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HOO03511.1"
                     /translation="MRNAVVEALANDGFLLDQEAEEFILGQPSPLQFARQAISQLPYQ
                     PLVVTMRDLRTVVRVEAPRLETVPRQEPLPSRRLGEVEVLKDITSHSDCLATVEGFAR
                     YFQDRFASLKGLLLRRRDMLGAISIQRAMDPRKLQADEVKVIGMVNEVRETRSGERIV
                     ELEDDTGRMTVMVPKDSKAAKDSILQDEVVGAVGRMSRKDRKLILKELIRPDVPFGSG
                     MEVTDSTAQVAFMSDVHVGSNTFLEGRWQSMMAWLKEEAEELDIRYIVIPGDCVDGVG
                     VFPDQEEELVIEDIIDQYRALAEKLKEVPDGIRIVMQPGNHDMVRPAEPQPALGEEAA
                     RLFDSSILQVGNPAYLRIEGRTILSYHGKSFDDLMSGIKGLSYSQPIKAMEEMLKRRH
                     LAPAYGGKTPFAPERRDYLVIDEVPDIFVTGHVHGAGVSFYNGVRLINASTWQDQTSY
                     QRRLNFVPEPAKLTLVHLGTGRPAVAEF"
     gene            complement(3237..3359)
                     /locus_tag="PLJ11_02195"
     CDS             complement(3237..3359)
                     /locus_tag="PLJ11_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03512.1"
                     /translation="MRLERLFDTPEKKARLAKYAWLISLLMLALGYALIIVLWD"
     gene            complement(3356..3991)
                     /locus_tag="PLJ11_02200"
     CDS             complement(3356..3991)
                     /locus_tag="PLJ11_02200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOO03513.1"
                     /translation="MPAADVKPTRSELLEIKKRIKLTESGYKILKMKRDGLILEFFKI
                     LEQAKDIRDKVNHAYDEAMASIAVAKAVDGAIAVQSAAFALRVHPELALRSKNIMGIV
                     VPEIEASSIRTPMDKRGYGVIGTSVYIDEAAKAFEDLVETIIEAAEIETTMKKILEEV
                     EKTKRRVNALEFKVIPELKETEAFIRLRLEEMERENTFRLKRVKQKAEARG"
     gene            complement(3993..5378)
                     /locus_tag="PLJ11_02205"
     CDS             complement(3993..5378)
                     /locus_tag="PLJ11_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOO03514.1"
                     /translation="MTSKEYRSITQIAGPLVFVEKTEPVGYNELAMVALPDGSERMGQ
                     VLDSSDEIVVIQMFEGTTGIPKTAGVKFLGETMKMPVSRDMLGRILSGSGKPLDGGPA
                     IVPEDRLEIIGAAINPWARDNPQEFIQTGISTIDGMNTLVRGQKLPIFSASGLPHNEI
                     ALQIARQAKVLSGAEDFAVVFAAMGITNEEAQYFMRDFERTGALKRAVVFLNLADDPA
                     IERIITPKLALTTAEYLAFTLDMHVLVILTDLTNYCEALRQIGAAREEVPGRRGYPGY
                     MYTDLASIYERAGRIKGKKGSITQIPILSMPGDDITHPIPDLTGYITEGQIVAKRELV
                     RAGIYPPIDVGSSLSRLMNEGIGKGRTREDHKAVSDQCYAAYAEGRDLRGLVAIVGKE
                     ALSERDRKFLEFADEFERRLVQQGREEDRSIETTLDLMWELLASLDERQLTRIDRKYI
                     EKYHPKYRKKE"
     gene            complement(5379..7109)
                     /locus_tag="PLJ11_02210"
     CDS             complement(5379..7109)
                     /locus_tag="PLJ11_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOO03515.1"
                     /translation="MGKEGVIYRVAGPVVTAVGISPRMYDVILVGKEQLMGEVIKIVG
                     DKTIVQVYEDTSGVRPGEKVVDTGQPLVAELGPGLLSSVYDGIQRPLPVLRDRMGDFI
                     ERGVSAPGLDRSLKWPFTPVVKKGDTVAEGQVIGTVPERAITHKVMVPPGIGGKVDMI
                     KEGKFTVDEVVAVIGGKEVRMMQKWPVRRGRPFARKMAPEEPLITGQRVLDTLFPLTK
                     GGTGAIPGGFGTGKTVTQQQLAKWSDAEIVVYIGCGERGNEMTEVLTSFPELTDPKTG
                     KPLMNRTVLIANTSNMPVAAREASVYTGMTIAEYFRDMGLNVSLMADSTSRWAEAMRE
                     ISSRLEEMPGEEGYPAYLSARLSEFYERAGRVVSLSGETGSISVVGAVSPPGGDLSEP
                     VTQGTLRIVRVFWALDTKLRERRHFPAINWLTSYSLYSGQLDGWYRQNVAEDFPALRN
                     WAIGLLQKEAELQEIVQLVGSDALPDEQKLTLEISRMVREIFLQQNAYHPVDTYSPMS
                     RQYTFLKLINRFATNAAKAVENEVPVETIANMELRNRLAKSKFEENIEEELKAIAEAM
                     DKEFEALGGK"
     gene            complement(7120..7419)
                     /locus_tag="PLJ11_02215"
     CDS             complement(7120..7419)
                     /locus_tag="PLJ11_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOO03516.1"
                     /translation="MDIAVLGSDEFVLGFRLGGVKRIYTAKPNEYEAKALELIGDSSI
                     GVLAIDSSDLGLLSPTARRKVMESIAPVVVMVGRAESDLREKVKRAIGVDLYKTD"
     gene            complement(7419..8477)
                     /gene="ahaC"
                     /locus_tag="PLJ11_02220"
     CDS             complement(7419..8477)
                     /gene="ahaC"
                     /locus_tag="PLJ11_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase A1 subunit C"
                     /protein_id="HOO03517.1"
                     /translation="MLSWGSKGNYSYVTARVKAKKSLLISRDNYPKLVLMDLNEIGRF
                     LGETQYQVEMTELATKYDGVNLIELGTSRNLARVFTDILDYSKGELHDMLEHYLMRWD
                     IWNIKTVLRGKYYGAATEDIQEDIVAAGRFDESYINKLISLEDIEAVLDELGSREGLT
                     IPEEVLKDYQETKTLAPIEDYLDKRYYTDVLACVKCRDKAERLFKQFLRKEVDVTNLM
                     TLMKLKREGLSPESPERYFIEGGEELDIKTLVELSKAEDLGQLIGGLTKYSFYPEVKD
                     ALEEHKRTGSLSPVGLALQRHLLRSSEKFSHIYPLSVLPIIDYMLRKKQEVDNIRIIA
                     RCKESDLAPDQIKKLLVM"
     gene            complement(8482..9039)
                     /locus_tag="PLJ11_02225"
     CDS             complement(8482..9039)
                     /locus_tag="PLJ11_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOO03518.1"
                     /translation="MALDEVIESVMESAKQKAEALQQEAEKERAAILKQADDKIAAKR
                     LAQQKELEAALKRLRQQEISSAELEAKRIVLNAKKDVMDRSFQVALDSLLSMDEGNRG
                     RMYRKILENGKTVIRSPKVYCPRGDARLISGVAGLGKVEETDMEPGLILEDASGKVRL
                     DYRFRVVLEGVWDKELKNVSNILFG"
     gene            complement(9086..9289)
                     /locus_tag="PLJ11_02230"
     CDS             complement(9086..9289)
                     /locus_tag="PLJ11_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOO03519.1"
                     /translation="MIAIGAGIAVGITGLASAWAEKEIGSAAIGAMAENEKLFGKGLI
                     LTVIPETIVIFGMVVAILLWLNM"
     gene            complement(9312..>10340)
                     /locus_tag="PLJ11_02235"
     CDS             complement(9312..>10340)
                     /locus_tag="PLJ11_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOO03520.1"
                     /translation="YMERMLSTPKYNEIDPTVTMMITIPIFFGLMVGDIGYGIPFMIL
                     GWLGLRNCRSDEWRTISTLLFYGGIWATIFGVFLFGEMYGIHFAEQPHDMSWSSLLGV
                     QLPHEIAIGGFEIPLGIYSKLHNVKPILYMTIWIGLIHVFIGYVIGVYNKAIRFGLKH
                     AILERFSWLLIVIGGALLLLVVVDAMILGKTVELTDLRILIAAPLIVVGILLAYKGEG
                     AAAILDLPGLMGNIISYTRLAAIGMSKAGLALAFNTIAFEVLWGHDFLVPGSNSGDTI
                     ILIAALAIFLVGHLTIFILAIISAGLHSLRLHYVEFFMKFYEGGGVDFKPLKVIRKYT
                     IEKNGVSE"
BASE COUNT         1687 a         3350 c         3243 g         2060 t
ORIGIN      
        1 atcctctaca tcggcctgcc gctgggcgcg gtctatatcc tcaaggggga gggcgccccc
       61 taccgccgct atatcctcgg gggcaacttc cggatcgggc gctgggagaa gctcttcctg
      121 gtgctgtcgt ccgccatctt cgccctggcc catgtcttca gctgggacct gtataaggtc
      181 cccccgacgt tcctggccgg tctggcgctg ggctacctct tcctgcggta cggggtgtat
      241 gccagcataa tgctgcactt caccatcgac tacctgagcc tgccgatgga ggtgtggccg
      301 ggcgccggga cggagctggt catggggctc tttgtcctgg tgctcaacgt cgtcggcgcc
      361 gtgtacctcg tctattattc cgtccgggcg gtagagctgt tcagtggccg caagctctgg
      421 aactgggggg aggagcggcc ggtgagcgcc tactatcggc ccctgtcgcc gaaggcgcag
      481 gccgtcgcga cccccgcctt cgggttcgtg tgcaagcatt gcgcgaacac cgaggccagg
      541 tacgtggacg gcaggttcgt ctgcacccgg tgcggccggg aaagctgacg ggggaacgtg
      601 ttcacttcat cgaggccagg atggcctcca gcctgccttt ggagacgagg gaccggtcga
      661 gttcgcgggc ctcgatgacg taccttcccc cgtacgggcg gaacgccctg agccatcgcc
      721 cgaggtcgat gttgccctcc ccgatcacga ggtgccggtc gcggtcgccg cagttgtcgt
      781 gcacgtgcat gttgatgaag cggtccttga tcgagaggaa ggcgggaatg ttgccgacgg
      841 tgttggcatg cccgatgtcg aagcacaccc ccagccccgt cccttcgatg agccgtgtga
      901 gttcctcggg ccccttggcc agggccgcgg gcatctccgg catgttctcc aaggccaccc
      961 tcaggccgag gtcctccctc gccgcgtcga tcgccctgag ggaggcgagc gtggcgcgca
     1021 cggcggcctc ccggtccagg gccgccaggg gggagagaag cccgggatgc acggtcacca
     1081 ggtcgatgcc catggcgtgc gcgagccgga ggcattccag cacctcgtcc agggcggcct
     1141 gcctcatccg cggggtgagg gcgcctatgt tcacgtcgct gaggggcgcg tggaccgacc
     1201 actcgaggtc ataggagggg gagagttcga ggaacgccgg tttgatggcg gcgagggagt
     1261 gcctcccctc ggccacgatc tcccaggcct ggtaatgctc cgccaccttg gcgatggcca
     1321 gggggaactc ccaatgcgac aggtgcggcg aggacacggc gatcatggga cctccccgtc
     1381 gaccgtgcgg ggcgcgacgt cgtctatcag cgcctcgagg gcgtcgtctg cgacgagcac
     1441 ctcgatcgcc cccagcggtg ccccgccctc gacgaacgag acctttccga cgaacgcggc
     1501 ctgcttgttg agctggaacc tgagcccgtc ctcggagagg ccgcggagca ggaccgcgcg
     1561 ggcgctgtcc aggatcctgt ggttccggag gacctccctg aagcggtcta ggtcccgggc
     1621 cgtggcgacg agcacgtccc cgtcataggc cgtctccgcc gtggggaaga tgttgaggag
     1681 ggcgcggcgc accttctccc cgtcctcggt cgggaacacc ctggccttgg ccgtgacgat
     1741 catcggggag ggatattttg agaaactatt taaaatgggg gcggcatggc aaggaaggat
     1801 gcgcaacgcg gtcgtggagg cactggccaa tgacggcttt cttctggacc aggaggccga
     1861 ggagttcatt ctggggcagc cctccccgct gcagttcgcc cggcaggcga tctcccagct
     1921 gccgtaccag cccctggtgg tcacgatgcg cgacctgcgc accgtggtca gggtcgaggc
     1981 gccgcggctg gagacggtgc cgaggcagga gcccctgccg agcaggcgcc tcggcgaggt
     2041 ggaggtgctg aaggacataa cctcgcactc ggactgcctg gcgaccgtgg agggcttcgc
     2101 ccgctacttc caggaccgct tcgcctccct caaggggctc ctcctccgcc gccgggacat
     2161 gctcggggcc atatcgatcc agagggcgat ggacccgagg aagctccagg cggacgaggt
     2221 caaggtcatc ggcatggtga acgaggtccg cgagaccagg tccggggaga ggatcgtcga
     2281 gctcgaggac gacacgggcc ggatgaccgt catggtgccc aaggacagca aggccgccaa
     2341 ggactccatc ctccaggacg aggtcgtcgg ggcggtcggc aggatgtccc gcaaggaccg
     2401 caagctgatc ctgaaggagc tgatccggcc ggacgtgccg ttcggctcgg ggatggaggt
     2461 caccgattcc accgcccagg tggcgttcat gtcggacgtg cacgtgggga gcaacacctt
     2521 cctcgagggc cggtggcaga gcatgatggc ctggctcaag gaggaggcgg aggagctgga
     2581 catccgctac atcgtgatcc cgggggactg cgtcgacggg gtcggcgtgt tcccggacca
     2641 ggaggaggag ctggtcatcg aggacatcat cgatcagtat agggcgctgg cggagaagct
     2701 caaggaggtg cccgacggca tccgcatcgt catgcagccc gggaaccacg acatggtgcg
     2761 ccccgcggag ccccagcccg ccctggggga ggaggccgcc cgcctcttcg actccagcat
     2821 cctgcaggtc ggcaacccgg cctacctgcg gatcgagggg aggaccatcc tctcctacca
     2881 cgggaagagc ttcgacgacc tgatgagcgg catcaagggg ctgtcgtact cgcagccgat
     2941 caaggccatg gaggagatgc tcaagcgcag gcacctcgcc ccggcgtacg gggggaagac
     3001 ccccttcgcc ccggagagga gggactacct ggtcatcgac gaggtgccgg acatcttcgt
     3061 cacggggcac gtgcacgggg cgggcgtctc gttctacaac ggcgtgcggc tgataaacgc
     3121 ctccacctgg caggaccaga cctcgtacca gcggcggctg aacttcgtcc cggagccggc
     3181 gaagctgacc ctggtgcacc tgggcacggg caggccggcg gtggcggagt tctagctcag
     3241 tcccagagga cgatgatgag ggcgtacccc agggcgagca tcaggaggga gatgagccag
     3301 gcatacttgg ccagcctggc cttcttctcg ggggtgtcga acagcctttc gagcctcatc
     3361 cgcgcgcttc cgccttctgc ttcacccgct tcagccggaa ggtgttctcc cgctccatct
     3421 cctcgaggcg gaggcggatg aaggcctccg tctccttcag ctccgggatc accttgaact
     3481 cgagggcgtt gacgcggcgc ttggtcttct ccacctcctc caggatcttc ttcatggtgg
     3541 tctcgatctc cgccgcctcg atgatcgtct cgaccaggtc ctcgaaggcc ttggccgcct
     3601 cgtcgatgta gacgctcgtc cctatgaccc catatccccg cttgtccatg ggggtgcgga
     3661 tggaactcgc ctcgatctcc gggaccacga tgcccatgat gttcttggag cgcagcgcca
     3721 gctccgggtg gaccctcagg gcgaaggcgg cgctctgcac cgctatggcc ccgtcgaccg
     3781 ccttggccac ggctatgctc gccatcgcct cgtcgtaggc atggttgacc ttgtcccgga
     3841 tgtccttcgc ctgctccagg atcttgaaga actccaggat caggccgtcc cgcttcatct
     3901 tgaggatctt gtacccggac tcggtcagct tgatcctctt cttgatctcc aggagctcgg
     3961 agcgggtcgg cttaacatcg gctgcgggca tctcactcct tcttgcggta cttcgggtgg
     4021 tacttctcga tgtacttgcg gtctatcctg gtcagctgcc tctcgtccag cgatgccagg
     4081 agctcccaca tcaggtcgag ggtggtctcg atggacctgt cctcctccct cccctgctgc
     4141 accaggcgcc gctcgaactc gtccgcgaac tccaggaact tgcggtccct ttccgacagg
     4201 gcctccttgc cgacgatggc gaccagcccg cgcaggtcgc gcccctcggc gtaggcggcg
     4261 tagcattggt cggagacggc cttgtggtcc tcccgcgtcc tgcccttccc tataccctcg
     4321 ttcatcagcc gggagaggga ggagccgacg tcgatcggcg ggtagatgcc cgcccgcact
     4381 agctccctct tcgccacgat ctggccctcg gtgatgtacc ccgtcaggtc ggggatcgga
     4441 tgggtgatgt cgtccccggg catggatagg atggggatct gggtgatgct gcccttcttg
     4501 cccttgatcc tccctgcgcg ctcgtagatg ctcgccaggt cggtgtacat gtacccggga
     4561 tagccccgcc ggccgggcac ctcctcccgg gccgcgccga tctgcctgag cgcctcgcag
     4621 tagttggtca ggtccgtcaa tatgaccagg acatgcatgt ccagggtgaa ggccaggtac
     4681 tccgcggtgg tcagggcgag cttcggggtg atgatccgct cgatggccgg atcgtccgcc
     4741 aggttcagga acaccaccgc gcgcttcagc gccccggtcc tctcgaagtc ccgcatgaag
     4801 tactgggcct cctcgttggt gatccccatg gcggcgaaca ccacggcgaa gtcctccgcc
     4861 ccgctgagca ccttggcctg ccgcgcgatc tgtagggcga tctcgttatg cgggaggccg
     4921 gaggcggaga agatcggcag cttctggcca cgcaccagcg tgttcatgcc gtcgatggtc
     4981 gagatcccgg tctggatgaa ctcctggggg ttgtccctgg cccaggggtt gatggccgcg
     5041 ccgatgatct ccaggcggtc ctcgggcacg atggccgggc cgccgtccag cggcttcccg
     5101 ctcccggaga ggatcctgcc gagcatgtcc ctggacacgg gcatcttcat cgtctcgccg
     5161 aggaacttga cgccggcggt cttggggatc ccggtggtgc cctcgaacat ctggatgacc
     5221 acgatctcgt ccgaggaatc gagcacttgg cccatcctct cgctgccgtc gggcagggcc
     5281 accatggcca attcgttgta tcccacgggc tcggtcttct ccacgaagac caggggcccg
     5341 gcgatctggg tgatggaacg gtactccttg gatgtcattt acttgcctcc cagggcctcg
     5401 aactccttgt ccatggcctc ggcgatggcc ttgagctcct cctcgatgtt ctcctcgaac
     5461 ttcgatttgg ccaggcggtt gcgcagctcc atattggcga tcgtttccac gggcacctcg
     5521 ttctccaccg ccttggcggc gttggtggcg aacctgttga tgagcttcag gaaggtgtac
     5581 tgcctggaca tcggcgagta ggtgtcgacg gggtgatagg cgttctgctg gaggaatatc
     5641 tcccggacca tcctgctgat ctcaagggtc agcttctgct cgtccggcag ggcgtcggag
     5701 ccgaccagtt gcacgatctc ctgcagctcg gcctccttct ggagcaggcc gatggcccag
     5761 ttgcgcaggg cggggaagtc ctcggccacg ttctgcctgt accagccgtc cagctggcct
     5821 gagtacaggg aataggatgt cagccagttg atcgccggga agtgcctcct ctccctcagc
     5881 ttggtgtcga gggcccagaa gacccggacg atacgcaggg tgccctgggt gacgggctcg
     5941 gacaggtccc cgcccggcgg ggagaccgcc ccgacgacgg agatggagcc ggtctcaccg
     6001 gacagggaga ccacgcgccc ggccctctcg tagaactcgg agagccttgc ggacaggtag
     6061 gcggggtagc cctcctcccc gggcatctcc tccagccggg aggagatctc cctcatcgcc
     6121 tcggcccacc tggaggtgga gtcggccatc agcgagacgt tcaggcccat gtcgcggaag
     6181 tactccgcga tggtcatgcc ggtgtagacg gacgcctccc gggcggccac gggcatgttg
     6241 gacgtgttcg cgatcagcac ggtcctgttc atcaggggct tgccggtctt cgggtccgtc
     6301 agctccggga aggaagtcag gacctcggtc atctcgttgc cgcgctcccc gcaaccgata
     6361 tagaccacta tctcggcgtc ggaccacttg gccagctgct gctgggtgac cgtcttcccc
     6421 gtcccgaagc cgccggggat ggcgcctgtg ccccccttgg tgagcgggaa cagggtgtcg
     6481 aggacgcgct gtccggtgat gaggggctcc tcgggggcca tcttcctggc gaaggggcgg
     6541 ccgcggcgca caggccactt ctgcatcatc cggacctcct tgccaccgat gaccgccacg
     6601 acctcgtcca cggtgaactt tccttctttg atcatgtcca ccttgccgcc gatgcctggg
     6661 ggcaccatga ccttgtgagt gatggcgcgc tcaggcacgg tgccgatgac ctgcccctcg
     6721 gcgacggtat cccccttctt caccacgggg gtgaagggcc actttaagct ccggtcgagc
     6781 cccggggcgg agacccccct ttctatgaag tcccccatcc tgtccctgag gacaggcagc
     6841 ggcctctgga tgccgtcgta cacgctggat agaaggccgg gccccagctc ggccacgagg
     6901 ggctgcccgg tatcgacgac cttctctcct gggcgcacgc cggacgtatc ctcgtacacc
     6961 tgcactatgg tcttgtcgcc cacgatcttg atgacctccc ccatgagctg ctccttgccc
     7021 accaggataa cgtcgtacat cctaggcgaa atgccgaccg ccgtgacgac agggcctgca
     7081 actctataga tcacgccttc ctttcccatt tcaaacccct caatcagttt tgtacagatc
     7141 aacaccgatg gcccgcttga ccttctccct gaggtcgctc tcggccctgc ccaccatcac
     7201 cacgacgggg gcgatgctct ccattacctt cctccgggcg gtggggctca agagccccag
     7261 gtcggaggag tcgatcgcaa gtacgccaat gctgctgtcg ccgatcagtt ccagcgcctt
     7321 ggcctcatat tcgttgggct tggcggtgta gatccgcttc accccgccca gccggaaccc
     7381 caacacgaac tcatcgcttc caagcaccgc tatgtccatc acatcaccag caacttcttt
     7441 atctggtccg gtgccagatc gctctccttg catctcgcga tgatccgtat gttgtccact
     7501 tcctgcttct tccttagcat gtagtcgatt atgggcagaa cggacagcgg gtagatgtgc
     7561 gagaacttct cagagcttcg gaggaggtga cgctgcaacg ccaggcccac ggggctcagc
     7621 gagccggtcc gcttgtgctc ctccaatgcg tcctttacct ctggatagaa cgaatacttg
     7681 gttagcccgc cgatgagctg gccaaggtcc tccgccttgc tgagctcgac cagcgttttg
     7741 atgtccagct cctcgccgcc ctcgatgaag tacctctccg ggctttccgg ggagagccct
     7801 tcccgcttga gcttcatcag ggtcattagg ttggtgacat ccacctcctt gcgcaggaac
     7861 tgcttgaaca gcctctccgc cttgtcccga cacttgacgc aggccaggac atcggtgtaa
     7921 tagcgtttgt ccagatagtc ctcgatgggg gccagggtct tggtctcctg gtagtccttg
     7981 aggacctcct ccgggatcgt caggccctcc ctgctcccga gttcgtcgag caccgcctcg
     8041 atgtcctcga gggagatgag cttgttgatg tagctctcgt cgaacctgcc cgcggcaacg
     8101 atgtcctcct ggatgtcctc tgtcgcggcc ccatagtact tcccgcgcag gacagtcttg
     8161 atgttccaga tgtcccagcg catcaggtag tgctccagca tgtcatggag ctctcccttg
     8221 ctgtaatcaa ggatgtctgt gaacaccctt gccaggttcc tgcttgtgcc gagctcgatg
     8281 agattgacgc cgtcgtactt ggtggccagc tcggtcatct cgacctggta ctgggtctcg
     8341 ccaaggaagc ggcctatctc gttcaggtcc atgagcacga gcttagggta gttgtccctc
     8401 gagatgagca ggctcttctt ggccttgacc ctcgcggtca cgtagctata attgcccttg
     8461 ctaccccatg acagcatcct ttcacccgaa cagtatgttc gagacgttct tcagctcctt
     8521 gtcccaaacg ccttccagaa cgacccggaa ccggtagtcc aaccgcacct tgcctgaggc
     8581 gtcctccagg atcagccctg gctccatgtc ggtctcctcc accttcccaa ggccggccac
     8641 gccgctgatc agcctggcgt cgccccgggg gcagtagacc ttgggggacc tgatgaccgt
     8701 cttgccgttc tccaggatct tgcggtacat ccggccccgg ttgccctcgt ccatgctgag
     8761 cagggaatcc agggcgacct ggaacgatct gtccatgacg tccttcttgg cgttcaggac
     8821 gatcctcttc gcttcgagct cggcactgga tatctcctgc tgtcggaggc gtttcagggc
     8881 agcctccagc tccttctgct gggccagcct cttcgcggcg atcttgtcgt cggcctgctt
     8941 gaggatggcg gcccgctcct tctccgcctc ctgttgaagc gcctccgcct tttgtttggc
     9001 gctttccatc acgctttcga tcacttcgtc caatgccatg cgtcggcctc ttgtcctttg
     9061 tatcctctgt cgagatcggc gccgctcaca tgttgagcca gaggaggatc gcgacgacca
     9121 tgccgaagat gacgattgtt tccgggatga cggtcaggat aaggcccttt ccgaagagct
     9181 tctcgttctc cgccatggcg ccgatggcgg cggagccgat ctccttctcc gcccaggcgg
     9241 aggccagacc ggttatgcct actgcgattc cagcaccgat tgctatcaag cctgcatctc
     9301 ccattgccat tttactcact cactccattc ttctctatcg tatacttccg aatcactttc
     9361 aagggtttga agtcaacacc gccgccctcg tagaacttca tgaagaactc cacgtagtgc
     9421 aacctgaggc tgtgcagccc ggccgatatt atcgcgagga tgaatatggt caggtgcccc
     9481 accaggaata ttgccagtgc ggcgatgagt atgatcgtgt ccccgctgtt cgaacccggg
     9541 accaggaagt cgtgccccca gaggacctcg aacgcgatgg tgttgaaggc cagggcaagg
     9601 ccggccttgg acatgcctat tgcggccaga cgggtgtatg atatgatgtt ccccatgagc
     9661 ccggggaggt cgaggatggc cgcggccccc tcccccttgt aggctaggag gatgcccacc
     9721 acgatgaggg gggcggcgat caggatgcgc aggtcggtta gctccaccgt cttgcccagg
     9781 atcatggcgt cgacgacgac gagcaacagg agcgcgccgc cgatcacgat caggagccag
     9841 ctgaaccgct ccaggattgc atgtttgagg ccgaaccgga tggccttgtt gtagaccccg
     9901 atgacgtacc cgatgaagac gtggatcagg ccgatccata tggtcatgta caggatgggc
     9961 ttgacattgt gcagcttgct gtagatgccc agggggatct cgaacccgcc gatggctatc
    10021 tcatgaggga gctgcacccc caatagggag gaccagctca tgtcgtgggg ctgctcggcg
    10081 aagtggatgc cgtacatctc accgaaaagg aacacgccga agatggtggc ccatatgccc
    10141 ccgtagaaca gcagggtgga gatggtccgc cactcgtcgg agcggcagtt cctgagccca
    10201 agccagccta ggatcatgaa cgggatgccg taccctatgt ccccgaccat cagcccgaag
    10261 aagatgggga tggtgatcat catggtcacg gtggggtcga tctcgttgta cttaggggtc
    10321 gacagcatcc tttccatgta
//
LOCUS       DAOSYN010000027         9786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_5906_length_9786_cov_4.781112, whole genome shotgun sequence.
ACCESSION   DAOSYN010000027 DAOSYN010000000
VERSION     DAOSYN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_9786_cov_4.781112"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLJ11_02240"
     CDS             <1..277
                     /locus_tag="PLJ11_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO03521.1"
                     /translation="AILLKAACLTPARTFGVNKGEIAEGRDADLVVVDPREIVRIKGK
                     ELHSKCGWTPYEGREALFPQAVFLRGRLVLRDGSVQEEGRGREVPYA"
     gene            270..1400
                     /locus_tag="PLJ11_02245"
     CDS             270..1400
                     /locus_tag="PLJ11_02245"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOO03522.1"
                     /translation="MPEGPLPPDLSELEGRRFVCEDPCKLCCLCQPECLPTEVEYFKR
                     NYPDHLVLKREPHRHYALSLKKGQGSCAFLNKGRCEVYAHRPHFCQQFPFHVHVGQRV
                     QVELDLSCRGVWGDKGEEALPEGLRLVQANEAEIRRTLREAIPVYREFNLNCLDAGLE
                     VDVGKVRSQVSARLDELTDLRLIASLLEASVEEDELNIQDLKAKPSLDEGTLRDLEHS
                     ALEMVAGSLGAEELFQAPVYCAPDGGWNLFRIEGARVERYVMDEEGDVHKAGGLPVKA
                     IRLQQPNGFDRRTLKDYLRVLNNRDSLMGYAFYLMDEFGYEDPWPNVYGGVLSTSVLD
                     LLWRTALLAAFFPGGKDGERMREGIIFYDMDRLDAPTLGAFV"
     gene            1479..1694
                     /locus_tag="PLJ11_02250"
     CDS             1479..1694
                     /locus_tag="PLJ11_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177702.1"
                     /note="Enables 3` processing of polyadenylated mRNAs and
                     tRNA precursors; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small nuclear ribonucleoprotein"
                     /protein_id="HOO03523.1"
                     /translation="MQKPLNVLNQAINSQVIVTLKGNREYRGVLDGYDPHMNVVLKNA
                     EELINNEVVRKLSVAIVRGDNVIYISP"
     gene            1705..1872
                     /locus_tag="PLJ11_02255"
     CDS             1705..1872
                     /locus_tag="PLJ11_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HOO03524.1"
                     /translation="MTKGTAAHGKMGSKKTHVPCRRCGKRAYNIRTSCCASCGYGKTT
                     TMRTYNWAKSH"
     gene            1890..3296
                     /gene="purF"
                     /locus_tag="PLJ11_02260"
     CDS             1890..3296
                     /gene="purF"
                     /locus_tag="PLJ11_02260"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOO03525.1"
                     /translation="MEDDRPKHFCGVVGMALEGDAVPHLKKALRIIQHRGQESAGIAV
                     STGGAISYVRGMGLVHEVLTGRQYNALAGNRGIGHVRYSTSGSSCAENCQPITVTTKE
                     GDLALAHNGDIVNAGSIRDKLQSEGWAFLTSSDSEIIVRMLATELSNSPDPVRAIKTV
                     MRTLEGAYSITMMLDGRVFGFRDPFGFRPLCLGRLPGGYALASESAVFDVLKGEFIRD
                     VQPGEIVEISSSGATSTRTPAAPHCAHCMFEWVYFARPDAVIEGREVYQVRTNIGHIL
                     AKEQPVEADVVVPVPDSGRAHALGFAEGSGIPYAEGFMKNRYIERTFILPDQSQRDEG
                     VLLKLNPIRSTVAGKRVVIVDDSIVRGTTMLKIVQMTRRAGAKEVHVRIGCPPVIAPC
                     FYGVDMKTREQFAALNRTPKQIAELITADSVGYISQAGLVRALGIPESELCLACVNGE
                     YPTRIAGEKMRFQRTLEV"
     gene            3488..3742
                     /locus_tag="PLJ11_02265"
     CDS             3488..3742
                     /locus_tag="PLJ11_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03526.1"
                     /translation="MAVGVASFVIGLIYLIAAYGFFKGWSWIWLVTMVVVVLGILVDI
                     VAWAIGGFDDLGGLLLGLIIPVIIVLYMNSQNVKAFFRRA"
     gene            3835..3907
                     /locus_tag="PLJ11_02270"
     tRNA            3835..3907
                     /locus_tag="PLJ11_02270"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3868..3870,aa:Ala,seq:ggc)
     gene            complement(3991..4905)
                     /locus_tag="PLJ11_02275"
     CDS             complement(3991..4905)
                     /locus_tag="PLJ11_02275"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOO03527.1"
                     /translation="MMPLSPDFKENILQSIGNTPLVRINRLNPNKDVTIYAKVEGFNP
                     TGSIKDRIALSMVQQAEEEGTLTEGKTIIEPTSGNTGVALAMIGAIKGYPVEIVMSDS
                     VSVERRQMIEAFGGKVILSEGRYGTDGAIRLARRLVRESPDKYFMPDQFSNQYNKLAH
                     YRTTGDEIWRQTGGNVDHFVSALGTSGTIMGVGKALREHNPDIKIVSAHPVRGHYIQG
                     LKNMEEAIVPAIYDPNQIDITIMVETEVAYEMTRQIVKQEGIFVGMSSGAAMYAALEM
                     AKRVDHGVIVTIFPDRGEKYLSTDLFRR"
     gene            5144..6034
                     /locus_tag="PLJ11_02280"
     CDS             5144..6034
                     /locus_tag="PLJ11_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO03528.1"
                     /translation="MIIDSHVHLWLRDHLPDAMVRMYLEPLAALEGLMDWDVSAEKVW
                     PEYTVDAEKALEAMDAGGIDKAVVLPIDFNLVERARIDIEGYNRWVFETCAQVPDRLI
                     PFIGVDPQRGEAALELLDRFVSKYDAKGVKLYPSTGWFPNEDRVRGFLARVDELGLTV
                     VSHTGATWGSLEERYSEPSYWTDVLERYPDTNIVLAHLGGKWRHQVIQMCKAYPNCYT
                     DCSALQGWLPSDPGTALARLKELAGSIPDKVSFGSDFPLFELSYTTPSWAYFVRTQPW
                     ADEEIKAKLLGENMRALLGL"
     gene            6084..6974
                     /locus_tag="PLJ11_02285"
     CDS             6084..6974
                     /locus_tag="PLJ11_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO03529.1"
                     /translation="MIIDGHVHMWLRPMYPDVIINAYLEPWLAMSEFLDLSRDMEDNF
                     PTSEVRPELLLGYMNRAKVDRSVVLPLDFGLVQEPKAGVEALNDWIFGSAQLYPDKLI
                     PFMGIDPRRGEQAIRLMRKYHSQFEPRGIKVYPPNGFYPNEERLRPFWDEVRDMDLIV
                     VSHAGASWGPLDEEKGRPIHFKGVLERYPDMKLIIAHLGGKYRAETYEMAREYENLYT
                     DCSALQGWLPSDPGTAKDRLGEALAVMGDKVIFGSDWPLFDLAYPYESWCAFVREQAF
                     CSEEQRERLMGGTMQRLLGL"
     gene            complement(6976..7542)
                     /locus_tag="PLJ11_02290"
     CDS             complement(6976..7542)
                     /locus_tag="PLJ11_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO03530.1"
                     /translation="MDVREAVYKRRALRALDTRPVPKAVVDEMVEAMRLSPSCNNNQP
                     WRVIVAREPGPLEAIKASLNRGNSWASRAPLIMAVCARLDDDCKLPDRRDYFLFSTGL
                     AVGEMLLRATELGLIAHPIGGYDALAIKERLGVPEDHVLIALVICGYPGTDTSLLSEK
                     QLAAERERPARRPVGENFFDGHWGRPWA"
     gene            7638..8171
                     /locus_tag="PLJ11_02295"
     CDS             7638..8171
                     /locus_tag="PLJ11_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03531.1"
                     /translation="MDLSIVEAGLVGGGLVAIASAVYGRKESSSWDEVLLFLGFLVGA
                     AMIGIGAWAWGIGEYDISTRLILIFLGLSLFLRAIKGIKLASLIALITGGAVGYGLYW
                     LGKAFELEFLTSTVILVTAFLVLIIVYVIFKFVEDILDMGGVVLGFRSVQFLVGLVAF
                     AEAVLLLLDRSLWYFLS"
     gene            8219..8319
                     /locus_tag="PLJ11_02300"
     tRNA            join(8219..8256,8281..8319)
                     /locus_tag="PLJ11_02300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8253..8255,aa:Arg,seq:tct)
     gene            complement(8373..9215)
                     /locus_tag="PLJ11_02305"
     CDS             complement(8373..9215)
                     /locus_tag="PLJ11_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOO03532.1"
                     /translation="MGKGKGSSLMSRAASGSPASDYLIPGENIKWQGKPSAIIIMGKG
                     LLLTAFALVYLVLMEFFHNSNDDLLILAIAALACLLMIVTDRKRFGLIGGLAGLALLT
                     WTVLIDDSLAWYLYLIPLVFALLLLIINYIYLSRVLFVVTDQRIITRYGIFTLRYADT
                     GIDKVQSVTTIQPWYERLFGYGDVFFATAGEKGGIDYESPGIKLMSGGAVSWENVGRP
                     FEVTKIASAVIHTDATPVMVMNQPEGRMSPAEKLDKIEDLRKRGIITQEEYEKERKEA
                     LEEL"
     gene            9606..9777
                     /locus_tag="PLJ11_02310"
     tRNA            join(9606..9643,9743..9777)
                     /locus_tag="PLJ11_02310"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9640..9642,aa:Trp,seq:cca)
BASE COUNT         1924 a         2943 c         2989 g         1930 t
ORIGIN      
        1 ggccatcctg ctcaaggccg cctgcctcac ccccgcccgc accttcggcg taaacaaggg
       61 ggagatcgca gagggccggg acgcggacct ggtcgtggtc gaccccaggg agatcgtgcg
      121 catcaaaggg aaggaactgc atagcaagtg cggctggacg ccgtacgagg gcagggaggc
      181 cctcttcccg caggcggtgt tcctccgcgg gaggctggtc ctcagggacg gttccgtcca
      241 ggaggagggg aggggaaggg aggtgcccta tgcctgaggg accgctcccc ccggacctga
      301 gcgaactgga gggaaggcgc ttcgtctgcg aggacccctg taagctctgc tgcctatgcc
      361 aaccggaatg cctcccgacc gaggtcgaat atttcaaaag gaactatcca gaccatctag
      421 tgctcaagag ggagccgcac cgccattacg ccctctcgct caagaaggga caaggctcct
      481 gcgccttctt gaacaaggga aggtgcgagg tctacgccca ccgcccccac ttctgccagc
      541 agttcccctt ccatgtccat gtcggacaga gggtgcaggt ggaactggac ctcagctgcc
      601 ggggcgtatg gggggacaag ggggaggagg cgctgccgga gggattgcgc ctggtgcagg
      661 cgaacgaggc cgagatacga cgcacgctga gggaggccat cccggtgtac cgcgagttca
      721 acctgaattg cctggatgcc ggcttggagg tggacgtcgg gaaggtgcgc tcccaggtgt
      781 ccgcccgcct ggacgagctc accgaccttc gcctgatcgc gtccctgctg gaggcgagcg
      841 tggaggagga cgagctcaac atccaggatc tcaaggcgaa gccctcattg gacgagggca
      901 cgctgaggga cctggaacat tcggccctgg agatggtggc cgggtcattg ggagccgagg
      961 agctgttcca ggcaccggtg tactgcgccc ccgacggagg gtggaacctc ttccggatcg
     1021 agggggcgag ggtggaacgg tacgtgatgg acgaggaggg ggacgtacac aaggccggag
     1081 gccttcctgt gaaggccata cgacttcagc agcctaatgg gttcgacagg aggacgttga
     1141 aggactacct gagggtgctg aacaaccgcg acagcctcat gggctacgcg ttctacctga
     1201 tggacgagtt tgggtacgag gacccctggc ccaacgtgta cgggggagtg ctctcgacgt
     1261 cggtgctcga cctgctgtgg cgcaccgcgc tgctggccgc cttcttcccc ggcggaaagg
     1321 acggggagcg catgcgggag ggcatcatct tctacgacat ggaccgcttg gacgccccga
     1381 cgctgggagc gttcgtgtga ctggccaagg cgcatcgagg gctaaggcgc aaaacttcat
     1441 atagtgtccg ttcattcgac cgctatggag atctgagcat gcagaagccc cttaacgtcc
     1501 tcaaccaggc gataaacagt caggtgatcg tcacgttgaa gggcaaccgg gagtaccgtg
     1561 gggtgctgga cggatacgac cctcacatga acgtcgtgct caagaacgct gaggagctca
     1621 tcaacaacga ggtcgtccgc aagctcagcg tcgccatcgt ccgcggagac aacgtgatct
     1681 acatctcccc ctgaggttac ccctatgacg aaaggaacgg cagcgcatgg aaagatgggc
     1741 agcaagaaga cgcacgtccc ctgccgcagg tgcggcaaga gggcctataa catccgcacc
     1801 tcctgctgcg cctcctgcgg gtacggcaag accaccacca tgagaacgta taattgggct
     1861 aagtcccatt gacgttcgag gtagctccct tggaggatga ccggcccaag catttctgtg
     1921 gagtggtcgg catggccttg gagggcgatg ccgttccgca tttgaagaag gcgcttcgga
     1981 tcatccagca tcgcggtcaa gagtccgcgg gcatcgccgt ttccactggc ggggccatat
     2041 cctacgttcg cggaatgggc ttagtccacg aggtcctgac gggccgccag tacaatgccc
     2101 tcgccgggaa caggggcatc ggccatgtcc gctactccac ctccggttcg tcctgcgccg
     2161 agaactgtca gcccataacg gtcaccacca aggaagggga tttggccctc gcgcacaacg
     2221 gcgacatcgt caatgctggc tcgatccggg acaagctgca gtccgaaggc tgggccttcc
     2281 tgacctccag cgactccgag atcatcgtgc ggatgctggc caccgagctc tccaacagtc
     2341 ctgacccggt ccgggcgatc aagacggtca tgaggaccct ggagggcgcc tactccataa
     2401 ccatgatgct tgacggtcgg gtcttcgggt tccgggaccc cttcgggttc aggccgctat
     2461 gcctaggcag gcttccaggc ggatacgcgc tcgcctccga gagcgccgtc ttcgacgtgc
     2521 ttaaggggga gttcatccgg gacgtccagc ccggggagat cgtggagatc tcttcctcag
     2581 gcgcgacctc cacccgcacc cccgccgcgc cccattgcgc ccactgcatg ttcgaatggg
     2641 tgtactttgc ccgccccgac gcggtcatag aggggcgcga ggtctaccag gtcaggacca
     2701 acatagggca tatcctcgcg aaggagcagc cggtggaggc cgatgtggtc gtccccgtcc
     2761 ccgactccgg gagggcgcat gccctgggct tcgcggaggg ctccggcatc ccgtacgccg
     2821 aagggttcat gaagaaccgc tacatcgagc ggacgttcat cctccccgac caatcccaga
     2881 gggacgaggg cgtcctactg aagttgaacc ccatccgctc cacggttgcc ggcaagcggg
     2941 tggtcatagt ggacgactcc atcgtccgcg ggaccacgat gctcaagatc gtgcagatga
     3001 cgcgccgggc cggggccaag gaggtccacg tccgcatcgg ctgtccgccc gtgatcgccc
     3061 cctgcttcta cggcgtggac atgaagacca gggagcagtt cgcggccctg aaccggaccc
     3121 cgaaacagat cgcggagctc atcacggcgg attccgtcgg ctatatcagc caggccgggc
     3181 tggtccgcgc cctgggaatc cctgagtcgg agctatgcct ggcctgtgtg aacggggagt
     3241 atccgacccg gatcgccggg gaaaagatgc gcttccagag gaccctggag gtctgagcca
     3301 aggacattcg gggatgggtc gaaaacaata ttatcctaga tacaaatcaa taggcatgac
     3361 caagcaacct caccgggaac gcccgctcgg cgtgaccgtg atcggcgtgc tgctgctcct
     3421 cgcagggctc ataaccatcc tatcaagcgt tctgatcgat tccttctagg acctggagaa
     3481 catagaggtg gccgtagggg tggcgtcctt tgtcatcggc ctcatctacc tgatcgcggc
     3541 gtacgggttc ttcaaaggat ggagctggat ctggctggtg acgatggtgg tcgtcgtcct
     3601 aggcattttg gtcgacatcg tcgcctgggc catcggcggg ttcgacgacc tgggcgggct
     3661 gctcctcggc ctgatcatcc cggtgatcat cgtcctgtac atgaacagtc agaacgtgaa
     3721 ggccttcttc aggagggcct gagcctttcc ccctcggcgc ctcggcggga gatattggcc
     3781 agccagggag taagataaga ttatatagag aaaacctgtt tgccattccc gcatgggcag
     3841 gtagatcagc tggaagatcg ctaccttggc atggtagagg ccgggggttc aaatcccccc
     3901 ctgtccactc cttcattctc tccgtccgtt cgcaccatcg ttcatgccaa gcgcagccca
     3961 cagggaccga tagcgccgcc ctgatgcggt ctacctccgg aaaaggtcgg tgctgagata
     4021 cttctcccct ctgtctggga agatggtcac gatgacccca tggtcgaccc tcttggccat
     4081 ctccaaggcg gcgtacatgg cggcgccgct gctcatgccg acgaagatgc cctcctgttt
     4141 gacgatctgg cgcgtcatct cataggccac ctcggtctcc accattatcg tgatgtctat
     4201 ttgattcgga tcgtagatgg ccggaacgat ggcctcctcc atgttcttca gcccttggat
     4261 gtagtgcccc ctcacgggat gggcgctgac gatcttgatg tccgggttat gctccctcaa
     4321 cgccttcccg acccccatta tggtcccgga ggtcccaagc gcggagacga aatggtcaac
     4381 gttcccaccg gtctgcctcc agatctcgtc ccccgtggtc ctgtagtgcg caagcttgtt
     4441 gtactggttg gagaactggt ccggcatgaa gtacttgtcc gggctctccc tgaccaatcg
     4501 cctggccagc cggatggcgc catcggtacc gtacctcccc tcgctcaata tgaccttccc
     4561 tccgaaggcc tcgatcatct gccttctctc gaccgatacg ctgtcgctca tcacgatctc
     4621 caccgggtac ccctttatgg cgccgatcat ggccaaggcc accccggtat tgcccgacgt
     4681 gggctcgatg atggtcttcc cctccgtcag cgtcccctcc tcttccgcct gctggaccat
     4741 acttagggct atcctatcct tgatgctgcc agtgggattg aacccctcca ccttggcata
     4801 gatggtcacg tccttgttgg ggttcaaccg gttgatcctc accaaggggg tgtttccgat
     4861 cgactggagg atgttctctt taaaatcggg cgacagtggc atcatggcat gctctcaggc
     4921 tagaagggaa aggaagctaa tataactatg acagggctgg ctgcggcatc gcccgaccga
     4981 tcccagcgac ggtgccaagc agatgtcgcc tggaagggtt ggaccgatca tcgcctcttg
     5041 catccaatga tggaaggggg cgctccatgg aacgaaaggc cgaccggagg gcgtgggccc
     5101 tctcggaaag gtattcatgc gcccgcgtcg ttccgttcgg tcaatgatca tcgacagcca
     5161 cgttcacctt tggctccggg accatctgcc ggacgcaatg gtccgaatgt acctggagcc
     5221 cctggccgcc ctggagggac tcatggactg ggatgtgtcc gcagagaagg tctggcccga
     5281 gtacacggtg gacgcggaga aggccttgga ggccatggac gcggggggca tcgacaaggc
     5341 cgtggtcctc cctatagact tcaacctcgt ggagagggcg cggatagata ttgaggggta
     5401 caatcggtgg gtcttcgaga cctgtgccca ggtgcccgat cggctgatcc cgttcatcgg
     5461 cgtggaccct cagcggggcg aggccgcgct ggaactcttg gaccggttcg tctccaagta
     5521 cgacgccaag ggggtcaagc tctacccgtc cacgggctgg ttcccgaacg aggacagggt
     5581 caggggcttc ctcgcccgcg tggatgagct cggccttaca gtggtctcgc acaccggcgc
     5641 cacctggggg tccctggagg aacggtacag cgagcccagc tactggacgg acgtccttga
     5701 gcgctacccc gacaccaaca tcgtgctcgc ccacctgggg ggtaaatggc ggcatcaggt
     5761 catccagatg tgcaaggcgt atccgaactg ctacaccgat tgctcggccc tgcagggatg
     5821 gcttccctcg gacccgggga cggcattggc ccgcctgaag gaactcgctg gctccatccc
     5881 ggacaaggtg tccttcggct ccgacttccc actgttcgag ctcagctaca caacgccctc
     5941 gtgggcgtac ttcgtccgga cacagccttg ggcggacgag gagatcaagg cgaagctcct
     6001 cggggagaac atgcgcgccc tccttggcct ctaaaaccac ggaccaataa ttatatccaa
     6061 ccggtccccc gattccattc ccgatgatca tcgacgggca tgtccacatg tggctacggc
     6121 cgatgtaccc cgatgtcata atcaatgcct atctggagcc gtggctggcc atgtcggagt
     6181 tcctggacct gagccgggac atggaggaca actttccgac gtccgaggtc agaccggagc
     6241 tgctgctcgg atatatgaac agagcgaagg tggaccgttc cgtcgtgctt cctctggact
     6301 tcggcctggt gcaggaaccg aaggccgggg tggaggccct caacgactgg atattcggat
     6361 cggcgcagct atacccggac aagttgatcc cgttcatggg catcgacccg aggcgggggg
     6421 agcaggccat aaggctgatg cgcaagtatc attcccagtt cgagcccagg ggcatcaagg
     6481 tctatcctcc gaacgggttc tatcccaatg aggagaggct gcgtcccttc tgggacgagg
     6541 tcagggacat ggacctcatc gtcgtgagcc acgcgggggc cagctggggc cctctggacg
     6601 aggagaaggg ccgccccatc catttcaagg gcgtgctcga aaggtatccc gacatgaagc
     6661 tcatcatagc ccatctgggc ggcaagtaca gggcggagac ctatgagatg gcgagggagt
     6721 acgagaacct gtacaccgat tgctcagcct tgcaaggctg gcttccctcg gacccgggca
     6781 cggccaagga ccgcctggga gaggcgttgg ctgtcatggg cgacaaggtc atcttcggat
     6841 cggattggcc gctcttcgac ctcgcctatc cctacgagtc ctggtgcgcc ttcgtgaggg
     6901 agcaggcctt ctgctccgag gagcaaaggg agcggttgat ggggggcacc atgcaaaggc
     6961 tgctgggcct ctgactcacg cccagggccg tccccagtgg ccatcgaaga agttctcccc
     7021 tactggcctt cgcgctggcc gctccctctc cgccgcaagc tgcttctccg acagcaggga
     7081 ggtgtccgtg ccgggatacc cgcatatgac cagggcgatg aggacgtggt cctccgggac
     7141 cccaagccgc tccttgatcg ccagggcatc gtaccctccg ataggatgtg ctatcagtcc
     7201 gagctcggtg gcccgcagca gcatctcccc gacggccaaa ccggtgctga acagaaagta
     7261 gtcccgccgg tcggggagct tgcagtcatc gtccaaccgg gcgcagaccg ccatgatcaa
     7321 cggcgctctc gaggcccagg agtttcccct gttcagcgag gccttgatgg cctccaacgg
     7381 gcccggctcc cttgccacga tcacccgcca aggctgattg ttgttgcatg acggggaaag
     7441 gcgcatcgcc tccaccatct cgtcgacgac cgccttcggc actggcctgg tgtccaaggc
     7501 cctcagcgcc ctccgcttat agaccgcctc ccgcacgtcc atgcctatcg gacgtacagg
     7561 gcgaacagag gttaatatcc ctttccagac caagccctta atacattccc gggcaataat
     7621 atcgaacgga tcgtatggtg gaccttagca tcgtcgaggc tgggctggtg ggcggagggc
     7681 tggttgcgat cgcaagcgcg gtgtacggaa ggaaggaatc atcctcatgg gacgaggtat
     7741 tgctgttcct agggttcctc gtcggggcgg ccatgatcgg gataggggcc tgggcgtggg
     7801 gcatcgggga atacgacatc tccaccaggc tgatcctgat ctttctgggg ctgtccctgt
     7861 tcctgagggc cataaagggc atcaagctgg cgtccctgat agcactgatc acgggagggg
     7921 cggtgggcta cgggctgtac tggttgggga aggccttcga gctggagttc ctgacatcca
     7981 ccgtcatcct cgtcacggcc ttcctggtcc tcatcatcgt ctacgtcatc ttcaagttcg
     8041 ttgaggacat cttggacatg ggcggggtgg tgctggggtt ccgatcagtg cagttcctcg
     8101 tcggactggt ggcgttcgcg gaggcggtgc tgctcctgct cgatcgttcc ctgtggtatt
     8161 tcctctcctg accgcaccat aagcttttaa atcaggtcgc gattattgcg acacgttcgg
     8221 gcccgtggct tagctggata tagcactggc cttctattcc ctgagggcag ggacgaagca
     8281 agtcagtggt cccgggttcg aatcccggcg ggcccgccaa tcgaacaata ataaataatt
     8341 tattaaaaaa ataaaaaaag atcggaggag gatcagagct cttccaaggc ctccttcctc
     8401 tccttctcgt actcttcctg ggtgattatg cccctcttcc tgaggtcctc gatcttgtcg
     8461 agcttctccg cagggctcat ccttccttca ggctggttca tcaccatgac cggggtggcg
     8521 tcggtgtgga tcaccgcact ggcgatcttg gtgacctcga aaggccttcc gacattctcc
     8581 caggagaccg ccccgccgct catcagcttg atccctgggg attcatagtc tatcccgccc
     8641 ttctcgccag cggtggcgaa gaacacatcc ccgtatccaa atagcctttc gtaccagggc
     8701 tggatcgtgg tcacgctctg cactttatcg attccagtgt ccgcatatct cagggtgaat
     8761 atgccatacc tggtgatgat cctctgatcg gtgaccacga agagcaccct gctaagatag
     8821 atataattga ttatcaggag cagcagcgca aagaccaaag gtatcaggta cagataccaa
     8881 gccaggctgt cgtcgatcag gacggtccag gtcaagaggg ccagaccggc aagccctccg
     8941 atcagtccga accgcttccg atcggtcacg atcatcagca agcatgccag ggcggcgatg
     9001 gcgaggatca ggaggtcatc gttgctgttg tggaagaatt ccatcagcac tagatacacc
     9061 aaggcgaagg ccgtaagcaa aagacctttg cccatgatga tgattgcgga gggcttcccc
     9121 tgccacttga tgttctctcc aggtattaga tagtccgagg ccggcgaacc gctcgccgcc
     9181 cgggacatca atgagcttcc cttacccttt cccattcctc gctcctccgt catccatttc
     9241 cgccccggcg caaataaaat ctcgacgcgg gatattgaaa tcaataggta tgtgggatat
     9301 aataatgatt ggcagttcgg agccagacct ccgcggcaca ccccgcgctc ttcacatctc
     9361 aaagttcatt gcatgttccc cctggcttga acccggacac cttgccaagg gccacagcga
     9421 cccattggcg aggaaaaggt tattaaatac ccattgaatc agggcagcta gtctggtgtg
     9481 tactgatgaa gagaagctca cgcgcctgga agaagaaggg gaagcagcgc tggaaatggc
     9541 gcaagaagaa gatgagaagg cgaaagcacg cccagaagat gcgtaagcgc taaagctgaa
     9601 catgtggggg tatggggtaa cacggtatcc tcgcgggctc cagttaatat ggagtgattg
     9661 ctaacgggtt tgctgaccgg tgatgaataa ctggagcgat gacctgttaa tttcgcgtgg
     9721 ggtcgctccc cacgtggtgg aaacccgctg atctgggttc aaatcccagt gcccccactt
     9781 ccttta
//
LOCUS       DAOSYN010000028         9754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_5946_length_9754_cov_3.223425, whole genome shotgun sequence.
ACCESSION   DAOSYN010000028 DAOSYN010000000
VERSION     DAOSYN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5946_length_9754_cov_3.223425"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLJ11_02315"
     CDS             complement(<1..508)
                     /locus_tag="PLJ11_02315"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOO03533.1"
                     /translation="MIGMDDKVDLDHMGAVMALPPAPLVLVGVADREWKERNIITVGM
                     FNVFSVRPPIIGIGVTTARYSYKLLEENDEFTVNIPGKDLLDKVIACGERSGAETDKF
                     KLAGLTPVKGKRTKAPRIAECLMNIEVKRTQSFEVGDHTWYLGEIKHTDIVQGYDRSQ
                     ALLYRDGEF"
     gene            complement(553..1077)
                     /locus_tag="PLJ11_02320"
     CDS             complement(553..1077)
                     /locus_tag="PLJ11_02320"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOO03534.1"
                     /translation="MDKSEVNPIMACRGFPAFPVVLVAVSDGKGNSNVVTIALVHMFS
                     FNPPVMGIGVSPARYTYELLEAAEDFSINVPSKDLVEEVLYCGQRSGREVNKFEECGL
                     EPRQGKRIRSPVIEECPVNLECVKRQRVDAGDHVWYLGEVVHAEMAKSVDRERALMYW
                     AGEFRVVGDVIRRR"
     gene            1232..2017
                     /locus_tag="PLJ11_02325"
     CDS             1232..2017
                     /locus_tag="PLJ11_02325"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOO03535.1"
                     /translation="MKARIGIIGGTGVYDPDMFELKETVRPHTPYGPASDEIQLGELN
                     GVPVAFLPRHGRGHAIPPHMINYRANIYALRQVGVERVVSPCAVGSLQEEYKPGEIVL
                     VDQFIDLTKGRRYTFFDGPRTVHISTADPFCPEMNALFAKEAKRAKIPVHAGGTYVCI
                     EGPRFSTRAESRMFKAFADVIGMTLAPECALAREMEMCYTSLATITDYDVWAEHPVDT
                     ATVLRIMAENADRVRTLLSRTLPKIPEERKKCSCGSALADAGA"
     gene            2147..2377
                     /locus_tag="PLJ11_02330"
     CDS             2147..2377
                     /locus_tag="PLJ11_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03536.1"
                     /translation="MTCYFRHLEHVFERAGLQIDIDNRKRVDMIIHEMAGVDYKDCTK
                     TWKAVQELLRDEDAFIERLRTECQARNIYLEY"
     gene            complement(2378..2761)
                     /gene="gcvH"
                     /locus_tag="PLJ11_02335"
     CDS             complement(2378..2761)
                     /gene="gcvH"
                     /locus_tag="PLJ11_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOO03537.1"
                     /translation="MSEVPEGLRYTKDHEWAKVEGDMVTVGITDHAQDELTEFVYIQL
                     PKEGAQIRKGDVLAVVESVKNTADVYAPVSGTVVEVNASLEGDPAIINRSPYADGWIA
                     RIRPADPSELGALMDAKGYRRHIGE"
     gene            2854..5040
                     /gene="hypF"
                     /locus_tag="PLJ11_02340"
     CDS             2854..5040
                     /gene="hypF"
                     /locus_tag="PLJ11_02340"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOO03538.1"
                     /translation="MRITVKGVVQGVGFRPTVHRLARAMGLHGYVQNNGSTVVIEVDG
                     DAEAFVRRLREALPPLARLDEVSLSPGLPDAALQAMGFRIMPSQQGAGGVAVPNDAAV
                     CPACLRELFDPGNRRYLYPFTNCTDCGARFTVIEGLPYDREQTSMRPFPMCPECQREY
                     ADPDDRRFHHQTITCPACGPRYYLLGRDGGRDYDAPIAKFAAGLHEGKIGVAKSWGGM
                     HLCCTLDQLSHMREWYRRKEKPFAIMVRDLGSLGRYGRPSAHDLELLTSAHRPIVLVP
                     KVDGEVTERVAPGLGNIGVFLPYTSMQHLLFHFLEEDALVMTSANVPGEPMVLQEADA
                     LALGADMYLMHDREIVNRCDDSVVRSFGNRTYFIRRSRGHIPSAIPLPVKGVAVGVGA
                     QESVAGALAFDGRLYATQYIGDASSPGVLEFLEGALRYLRRLLGAERLDAVGMDLHPG
                     HSTRRLARAWAEESGTEPVEVQHHHAHAASLLVESGLEEMVALTLDGTGYGDDGVAWG
                     GEVLLADLKGYRRLGHLAEVPLLGGERAVYDVRRLAFALAEMTGGGLDCFPEGEAEVL
                     RKMMRRSGRTTSFGRALDAISCYLGVCQYRSYDGEPAMKLERWLEEARCLDMVPTVRR
                     GDVIDTPAMFRTMMETGGSRADRAGSMVHALLEGLVDLAAEAAETEGVRHIGLTGGVS
                     YNHAISAWTKELVETRGLTFVCHDLVPNGDGGISTGQCAVALSRMG"
     gene            5244..6293
                     /locus_tag="PLJ11_02345"
     CDS             5244..6293
                     /locus_tag="PLJ11_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03539.1"
                     /translation="MRKLIILLDGAADERIPIFDNRTPLEAASKAFMDSVARDGKVGT
                     TDGKDYTHTFLLELLTGRPYEIPRGVIEAYGLELPIGEGRVAYRMTPAKVRDHNVEWY
                     YNITCDEEMFLQRAIIKNRPKLASMDPKILLYHGGKGVLTVKGDDILDLPKPPGQAKL
                     DGADLGPFEDFARGVQDSTGGITLLPWGGGLGKVAVDIRREITKVPDLAVISKSPSAL
                     GVGRFLGMQTHRVPGFKEGAKVAAQLLGREDVFLHIEETDDISHKCNPKDKLRLIEEI
                     DRELARYEKHFQRCEVALLIDHGASSVTGEHLLMRVPFAVRGPRWKGDGTASFHENGG
                     QHFPLGALLDTILED"
     gene            6383..7486
                     /locus_tag="PLJ11_02350"
     CDS             6383..7486
                     /locus_tag="PLJ11_02350"
                     /inference="COORDINATES: protein motif:HMM:NF003372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HOO03540.1"
                     /translation="MRKADIIYAFEEQAGRRFVDGVWGKVPTYTVVIGNTETAKVPGV
                     SAAGAVPEITDFTPAADVELLHYGRCKCIDGVPVTPTGVPTPGIISMSALQLAPMPTF
                     VINGGVRVRPHTPYFEIEGEPGEDIRTGRALKDPRRAYENALQLGRQLARGSPYLVIG
                     ESIAGGTTTALAVLLAMGYDGQGKVSSSMIDNPHSLKERIAREGIANAPFSAEEMRAD
                     PLKAVQAVGDPMMPAAAGVVAGAAESVPVLMAGGTQMAAVMALVKGLAPHVLENVALG
                     TTKWIVNDRSSDVRAIVRQVGEVPILAADLDFGPSRHEGLAVFEKGLVKEGVGAGGIA
                     IAAFLSSQGRIGAKTMLEKVEENYVRLMRIMGK"
     gene            complement(7473..8099)
                     /locus_tag="PLJ11_02355"
     CDS             complement(7473..8099)
                     /locus_tag="PLJ11_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOO03541.1"
                     /translation="MSVAALITAGGKGTRIAELGVEKPLAPLLGRPMVDIVLEQLMLV
                     DEVSAVYVSVSPNVPRTREHLEAMGVRVLETSGTEYCHDLIQAAEGIAEDHVLICPAD
                     LPLLRAELVADVVNEYLRRKERSLTVGIPLERALEFGVDVTFYECVDGRSLIPCGVSV
                     VDRQAMLTREYLPGGYYVADTLDVVLNVNTVRDLREAERELRARLTCP"
     gene            complement(8096..8866)
                     /gene="cobS"
                     /locus_tag="PLJ11_02360"
     CDS             complement(8096..8866)
                     /gene="cobS"
                     /locus_tag="PLJ11_02360"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOO03542.1"
                     /translation="MRAAVALFTVLPAKAREEDVDALSHRFHLITFIGLAYGLLAGGT
                     MYVAAEWLPPLAGAALALAAVQLLNRFLHFDGLSDLGDGLVCGGTPERKLAAMKDSKT
                     GAGGVGYVVTFTLLSLAALGSIKGRPELLFLPLIAEIMNKNAMVLCASAGRPREGLGN
                     AFVANTSGGQALASIVLSSLLAFGAVGLLDLALGHWDLGMAAALALVGALVSSLVGLY
                     MSRVAMRNFGAVNGDVLGATNEIARPIVLLVLLMAVGL"
     gene            complement(8872..>9754)
                     /locus_tag="PLJ11_02365"
     CDS             complement(8872..>9754)
                     /locus_tag="PLJ11_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HOO03543.1"
                     /translation="DKAFCAKGVEHNGSQAVALVSAGISNSIRAGEASREGSVPHRQV
                     GTINIVAVTDRPLADCGLANAIMTITEAKAAALQDHQIPGTGTTSDAVLIACPLEGER
                     CLWSGTGSDHGIALAMAVRSALGESIVNWKGANGNSVPFLRHLAIRGVTFDDMWAAVK
                     EMDALDPAWDEARVRRMFEAKLEGLAKDINVNALLTAALVLEDKGRYGQLYGLDEAKF
                     QEDPVHLLADELLGIALAEYIGGSKCVFEYTRYDKRKPGILATLGPFMDDIVASLIGA
                     TMSVIYSELLEGEGRLS"
BASE COUNT         1652 a         3228 c         3292 g         1582 t
ORIGIN      
        1 agaactcccc gtcccggtag agcagcgcct ggctgcggtc gtacccctgc acgatgtcgg
       61 tgtgctttat ctccccgagg taccaggtgt ggtcccccac ctcgaaggac tgcgtcctct
      121 tgacctcgat gttcatcagg cactcggcga tgcgcggggc cttggtccgc ttccccttta
      181 ccggggtcag gccggccagt ttgaacttgt cggtttccgc gccgctcctc tccccgcatg
      241 ctatcacctt gtccaggagg tccttccccg ggatgttcac ggtgaactcg tcgttctcct
      301 ccagcagctt gtagctgtag cgggcggtcg tcacccctat gccgatgatg ggcggacgga
      361 ccgagaacac attgaacatc cccaccgtga tgatgttcct ctccttccat tccctgtccg
      421 ccactcccac cagcacaagc ggtgccggcg ggagggccat caccgcgccc atgtggtcca
      481 ggtcgacctt gtcgtccatc ccgatcatcg tatcacatgt aatggaacgg gacggcgggc
      541 ctaataacct tttcaccgcc gccggatgac gtcccccacc acccggaact cgccggccca
      601 gtacatgagc gcccgctccc ggtcgacgct cttggccatt tcggcgtgca cgacctcgcc
      661 caggtaccag acgtggtccc cggcgtccac cctctggcgc ttgacgcact ccaggttgac
      721 cgggcactcc tcgatgaccg gggaccggat cctcttgccc tgccggggct cgaggccgca
      781 ttcctcgaac ttgttcacct cgcgcccgga gcgttggccg cagtaaagga cctcctccac
      841 caggtccttg ctcgggacgt tgatggagaa gtcctcggcg gcctccagca gctcataggt
      901 ataccgggcc ggggacaccc caatgcccat gaccggcggg ttgaaggaga acatatgcac
      961 gagggcgatg gtgaccacgt tgctgttccc cttgccgtcg ctcaccgcga cgagcacgac
     1021 cgggaacgcc gggaaccccc tgcacgccat gatgggattg acctcgctct tatccattgt
     1081 tacccctctc tggacatttt taaggatgta aaataagctt tgggggcttg gttcgttccc
     1141 ttggaaggtt ccccctgaaa atggccagcg ccgccagggc atgacgccgt tccctttatt
     1201 actcccgaat cgcctatcgt gccggagcac catgaaggct aggatcggca taatcggtgg
     1261 gaccggggtc tacgaccccg acatgttcga gctcaaggag accgttcgcc ctcatacccc
     1321 ctacgggccg gcctcggacg aaatccagct gggggaactc aacggggtcc cggtggcctt
     1381 cctcccccgg cacggccgcg ggcacgcgat ccccccgcac atgataaact accgggcgaa
     1441 catctacgcc ctgaggcagg tgggcgtgga gagggtggtc tccccctgcg cggtcggctc
     1501 cctgcaggaa gagtacaaac cgggcgagat cgtactcgtc gaccagttca tagacctcac
     1561 caagggaagg aggtacacgt tcttcgacgg gccgaggacc gtgcacatca gtacggccga
     1621 ccccttctgc ccggagatga acgcgctgtt cgcgaaggag gcgaagcggg cgaagatccc
     1681 ggttcacgcg ggcggcacgt acgtctgcat cgaggggccg cgcttctcca cgcgggcgga
     1741 gagcaggatg ttcaaggcgt tcgccgatgt catcggcatg accctggcgc cggagtgcgc
     1801 gctcgccagg gagatggaga tgtgctacac gagcctggcg acgatcaccg actacgacgt
     1861 ctgggcggag cacccggtgg acacggccac cgtcctgagg ataatggcgg agaacgccga
     1921 cagggtgcgc acgctgctgt ccaggaccct tccgaagatc cccgaggaga ggaagaagtg
     1981 ctcctgcggg agcgcgctgg ccgacgccgg ggcgtaaggg cgttgggcag gggctgcaac
     2041 cccgcacgcc gcgttccggg ccgggatccc tcgagggccc ggggacggcg cccttgccgg
     2101 cgggcgatga aagactttta ttccgatcaa cggcatacta ttccgcatga cctgctactt
     2161 ccggcacctc gagcatgtct tcgagagggc cggactgcag atagacatcg acaaccgcaa
     2221 gcgggtggac atgatcatcc acgagatggc gggcgttgac tacaaggact gcaccaagac
     2281 ctggaaggcg gtgcaggagc ttctcaggga cgaggacgcc ttcatagagc ggctgcggac
     2341 ggaatgccag gcccgcaaca tctacctgga gtactgatca ttcgccgatg tgccttctat
     2401 agcccttggc atccatgagc gcccctagct cggaggggtc ggccggcctg atcctggcga
     2461 tccatccgtc ggcgtagggg gaccggttga tgatagccgg gtccccctcg agcgacgcgt
     2521 tgacctccac caccgtcccg gacacagggg cgtacacgtc cgcggtgttc ttgacgctct
     2581 ccaccaccgc cagcacatca ccctttctta tctgggcgcc ctccttcgga agctggatgt
     2641 agacgaactc ggtcaactcg tcctgggcgt ggtcggtgat ccccacagtg accatgtcgc
     2701 cctcgacctt ggcccattcg tggtccttcg tatagcggag tccctccggt acctcgctca
     2761 tgctatccct gctcgcaacg gagtgcggat atttatgcct cgtggggccg ggaccgacaa
     2821 gggataaata gccggggctg gatggaaagg ggcatgcgca tcaccgtcaa gggcgtggtc
     2881 cagggggtcg gcttccggcc gacggtgcac cggctcgccc gggccatggg ccttcacggg
     2941 tacgtgcaga acaacggttc caccgtggtc atcgaggtcg acggggacgc ggaggccttc
     3001 gtccggaggc tccgggaggc gctgcccccg ctggcccggt tggacgaggt ctcgctctcc
     3061 ccggggctgc ccgacgccgc cctgcaggcc atgggcttca ggatcatgcc gagccagcag
     3121 ggcgccggag gggtggcggt tcccaacgac gccgcggtct gccccgcctg cctgagggag
     3181 ctgttcgacc ccggcaaccg ccgttacctc taccctttca ccaactgcac cgattgcggc
     3241 gcgcgcttca cggtcatcga gggcctcccc tacgaccgcg agcagacgtc catgcgcccg
     3301 ttcccgatgt gcccggagtg ccagcgcgag tacgccgacc cggacgaccg gaggttccac
     3361 catcagacca tcacctgccc ggcatgcggc ccccgctact accttctcgg ccgggacggg
     3421 gggagggact acgacgcccc catagccaag ttcgccgccg gccttcacga agggaagata
     3481 ggcgtggcca agagctgggg cgggatgcat ctgtgctgca cgttggacca gctatcgcac
     3541 atgcgggagt ggtaccgccg gaaggagaag cccttcgcga tcatggtccg ggacctaggg
     3601 tcgttgggac ggtacgggag gccgtccgcg cacgacctgg agctgctcac ctccgcccac
     3661 cgccccatcg tgctggtgcc gaaggtggac ggcgaggtca cggaacgggt ggcgccgggg
     3721 ctcgggaaca taggggtgtt ccttccgtac acctcgatgc agcacctgct gttccacttc
     3781 ctggaggagg acgccctggt gatgacctcc gccaacgtcc cgggcgagcc gatggtgctc
     3841 caggaggcgg acgcgctcgc cctcggggcg gacatgtacc tgatgcacga ccgggagatc
     3901 gtcaatcgct gcgacgattc agtggtgcgc tcctttggga acaggaccta cttcatccgc
     3961 aggtccaggg ggcacatccc ctcggccatc ccgcttccgg tgaagggcgt ggcggtgggg
     4021 gtcggcgcgc aggagagcgt ggccggggcg ctggcgttcg acgggcgcct ctacgccacg
     4081 cagtacatag gcgacgcttc ctcgccaggg gtgctcgagt tcctggaggg ggcgctgcgg
     4141 tacctgcggc gcctgctcgg cgcggaaagg ctggacgcgg tgggaatgga cctgcacccc
     4201 gggcattcca cccggcggct ggcgagggcg tgggcggagg agagcgggac cgagcccgtg
     4261 gaggtgcagc accatcacgc gcacgccgcc tcgctgctcg tcgagtcggg cctggaggag
     4321 atggtcgccc tgaccctcga cggcacgggg tacggcgacg acggggtcgc ctggggcgga
     4381 gaggtgctcc tcgccgacct gaagggctac cgccggcttg ggcacctggc cgaggtgccg
     4441 ctgctcgggg gggagagggc ggtgtacgac gtgcgccggc tggcgttcgc cctcgccgag
     4501 atgaccggcg gcggcctgga ctgcttcccg gagggggagg cggaggtcct ccgcaagatg
     4561 atgcgccgct ccgggcgcac cacctccttc gggagggccc tggacgccat ctcctgctac
     4621 ctgggcgtct gccagtacag gagctacgac ggggagccgg cgatgaagct ggagcggtgg
     4681 ctggaagagg ctcgatgcct cgatatggtg ccgaccgtcc ggaggggcga cgtcatcgac
     4741 acccccgcca tgttccggac gatgatggag accgggggca gcagggcgga ccgggcgggg
     4801 agcatggtcc acgcccttct cgagggcctc gtcgacctag ccgcggaggc ggcggagacg
     4861 gagggcgtca ggcacatcgg cctcaccggg ggggtgagct acaatcacgc catctccgcc
     4921 tggaccaagg aactggtcga aacgcgcggc ctcacgttcg tctgccacga cctcgtcccc
     4981 aacggcgacg ggggcatctc cacggggcag tgcgccgtcg ctttgtccag gatgggatga
     5041 gcccccgtca agatgcccac cgcacccgcc ctcccttcct cggatcgggg ctggatgggg
     5101 aaggtcgggg gggtccgatc gaccttcttc cgtcggagcg ttctcccatc gtctcgggcg
     5161 gcgggccggg ggaccattcg gttggagggt tcgaccaaca gtaattatat ggctacgccc
     5221 attcgaggac gtcagggctg gcgatgagaa agctgatcat cctgttggac ggcgcggccg
     5281 acgagcgcat cccgatattc gacaaccgca cccccctgga ggcggccagc aaggccttca
     5341 tggacagcgt ggccagggac gggaaggtgg gcaccaccga cgggaaggac tacacccaca
     5401 ccttcctgct ggagctcctg accggcaggc cgtacgagat cccgaggggg gtcatcgagg
     5461 cgtacgggct ggagctgccg atcggcgagg ggagagtggc ctaccgcatg acccccgcca
     5521 aggtgcgcga ccacaacgtg gaatggtact acaacatcac ctgcgacgag gagatgttcc
     5581 tgcagagggc cataatcaag aaccgcccca agctggcctc catggacccc aagatcctgc
     5641 tctatcacgg ggggaagggg gtgctgacgg tcaaggggga cgacatcctg gacctgccca
     5701 agcccccggg ccaggccaag ctcgacggcg ccgacctggg gccctttgag gatttcgcca
     5761 ggggcgtaca agacagcacc ggcggcatca ccctcctgcc ctggggaggg gggctgggca
     5821 aggtggcggt ggacataagg agggagataa cgaaggtccc ggacctcgcg gtcatctcca
     5881 agagcccctc cgccctgggc gtcggcaggt tcctgggcat gcagacccat cgggtgccgg
     5941 ggttcaagga gggggcgaag gtggccgccc agctattggg cagggaggac gtcttcctgc
     6001 atatagagga gacggacgac atcagccaca agtgcaaccc gaaggacaag ctgcggctga
     6061 tcgaggagat cgaccgggag ctggccaggt atgagaagca ttttcagagg tgcgaggtcg
     6121 ccctgctcat cgaccacggg gcctcctccg ttacgggcga gcacctgctg atgagggtcc
     6181 cctttgccgt gcgcgggccc agatggaagg gggacgggac ggcctccttc catgagaacg
     6241 gcgggcagca ctttccgttg ggggcccttc tggacaccat cctcgaggat tgagcgcccg
     6301 gaccaataga ttaaatattt tcttcgcatt gagttggatg atacagccaa ttcgatttgg
     6361 ttggcatcga ggggcggcga tcatgcgcaa ggctgacata atatacgcgt tcgaggagca
     6421 ggcgggaagg aggttcgtgg atggcgtttg gggcaaggtg cccacctaca cggtcgtcat
     6481 cggcaacacc gagacggcga aggtcccggg cgtttcggcc gccggggcgg tcccggagat
     6541 caccgacttc accccggcgg cggacgtcga actcctccat tacggccggt gcaagtgcat
     6601 cgacggcgtt ccggtcaccc cgacgggcgt gcccacgccc ggcataatat cgatgagcgc
     6661 gctgcagctg gcgcccatgc cgaccttcgt gatcaacggc ggcgtacggg tgaggccgca
     6721 caccccctac ttcgagatcg agggggagcc gggggaggat atccgcaccg gccgggcgct
     6781 caaggacccc cggcgggcgt acgagaacgc cctgcagctg ggccgtcagc tggcacgggg
     6841 gagcccctac ctggtgatag gggagagcat cgccgggggg acgacgaccg ccctcgcggt
     6901 cctgctcgca atgggctacg acggccaggg caaggtcagc agcagcatga tcgacaaccc
     6961 ccactccctg aaggagcgga tcgcccggga ggggatcgcc aacgcgccct tctccgcgga
     7021 ggagatgagg gctgaccccc tcaaggcggt gcaggccgtg ggcgacccga tgatgccggc
     7081 cgcggccggc gtggtggctg gggccgcgga gagcgtcccg gtcctcatgg ccggggggac
     7141 gcagatggcc gcggtcatgg cccttgtcaa ggggctcgcc ccccatgtcc tggagaacgt
     7201 cgcgctgggg accaccaaat ggatcgtgaa cgaccgcagc tcggacgtcc gggcgatcgt
     7261 gcggcaggtc ggcgaggtgc cgatcctggc cgccgacctg gacttcgggc cctccaggca
     7321 cgaggggctc gccgtcttcg agaaggggct ggtgaaggag ggggtgggcg ccggcgggat
     7381 agcgatcgct gccttcctga gctcgcaggg gaggatcggc gccaagacga tgctggagaa
     7441 ggtggaggag aactacgtcc gattgatgcg gatcatgggc aagtgaggcg ggcgcgcagc
     7501 tccctctcgg cctccctcag gtcccgtacg gtgttgacgt tcagcaccac gtccaaggta
     7561 tcggccacgt agtatccccc gggcagatac tcccgggtga gcatcgcctg gcggtccacg
     7621 acgctcaccc cgcaggggat cagcgagcgg ccgtccacgc attcgtagaa ggtcacgtcc
     7681 accccgaact ccagcgccct ctccagcggg atgccgaccg tcagggacct ttcctttctc
     7741 ctcaagtact cgttgaccac gtcggccact agctcggcgc gcagcagcgg caggtcggcc
     7801 gggcagatga ggacatggtc ctcggcgatc ccctccgccg cctggatcag gtcgtgacag
     7861 tactccgtcc ccgaggtctc gagcaccctg acgcccatgg cctccaggtg ctccctcgtc
     7921 ctcgggacgt tggggctcac cgacacgtac accgccgaga cctcgtccac cagcatcagc
     7981 tgctccagca cgatgtccac catcggcctc ccgaggaggg gggccagggg cttctccacc
     8041 cccagctccg cgatgcgcgt ccccttcccc ccggcggtga tgagggcggc cacgctcata
     8101 gccccaccgc catcagcagt acgaggagca cgatcggccg ggcgatctcg ttggtcgcgc
     8161 ccagcacgtc gccgttcacc gccccgaagt tccgcatggc caccctgctc atgtacagcc
     8221 cgacgaggga actgaccagg gcgcccacca gggcgagggc ggcggccatc cccaggtccc
     8281 agtgcccgag cgccaggtcg agcaggccca cggccccgaa ggccaagaga gaggagagga
     8341 cgatcgatgc gagcgcctgc cccccggagg tgttggcgac gaacgcgttg cccaatccct
     8401 ccctcggcct ccccgcggag gcgcagagga ccatggcgtt cttgttcatt atctccgcga
     8461 tcagcgggag gaagaggagt tccggccggc ccttgatgga gccgagggcg gcgaggctca
     8521 gcagggtgaa cgtgaccacg tagccgaccc ccccggcccc cgtcttggag tccttcatgg
     8581 cggccagctt cctttccggg gtgcctccgc acacgagccc gtcccccagg tcgctgagcc
     8641 cgtcgaagtg caggaacctg ttcagcagct ggaccgccgc cagggcgagg gcggcccccg
     8701 ccaggggggg cagccattcg gccgccacgt acatggtccc cccggcgagg aggccatagg
     8761 ccagccctat gaacgtgatc agatggaagc gatgggagag cgcgtccacg tcctcctccc
     8821 tggccttggc cgggaggacg gtgaagaggg cgacggccgc cctcagcgcg cctacgacag
     8881 tcgtccctct ccctccagga gctctgagta gatgacggac atggtcgccc ctatcagcga
     8941 ggcgacgatg tcgtccatga acgggccgag ggtggccaga atgcctggct tccgcttgtc
     9001 gtagcgggtg tactcgaaca cgcacttgga gccgccgatg tactcggcga gcgcaattcc
     9061 gagaagctcg tcggccagga ggtgcaccgg gtcctcctgg aacttggcct cgtcaaggcc
     9121 gtagagctgg ccgtaccgac ccttgtcctc caggaccagg gctgcggtga ggagcgcgtt
     9181 gacgttgatg tccttcgcca ggccctcgag cttggcctcg aacatgcgcc gcacccgcgc
     9241 ctcgtcccat gccgggtcga gggcgtccat ctccttgacc gcggcccaca tgtcgtcgaa
     9301 cgtgacgccc cgtatggcca ggtgccgcag gaagggcacg gagttgccat tggcgccctt
     9361 ccagttgaca atggactccc ccaaggcgga gcgcacggcc atggccaggg cgatgccgtg
     9421 gtcggagccg gtcccggacc acaggcacct ttccccttcc agcgggcagg cgatcagcac
     9481 agcgtcgctc gtggtgcccg tccccgggat ctggtggtcc tgcaatgcgg cggccttcgc
     9541 ctccgtgatg gtcatgatgg cgttggcgag cccgcaatcc gccagaggcc ggtcggtgac
     9601 cgccacgatg ttgatggtcc ccacctgccg atgcgggacg gacccctcgc gggaggcctc
     9661 ccccgcccgg atggagttgg agatgcctgc gctgaccagc gctaccgcct gggagccgtt
     9721 gtgctcaacc cccttggcgc agaacgcctt gtcc
//
LOCUS       DAOSYN010000029         9558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_6114_length_9558_cov_4.535831, whole genome shotgun sequence.
ACCESSION   DAOSYN010000029 DAOSYN010000000
VERSION     DAOSYN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6114_length_9558_cov_4.535831"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLJ11_02370"
     CDS             complement(<1..458)
                     /locus_tag="PLJ11_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOO03544.1"
                     /translation="MGENMRIGFVGAGNMAEAMMKGIISAGVATKEDMIASEIVPQRR
                     DYITRTLGVRTVSDAIEVVRSSDVIILAVKPQQVGPVLDELKPYLSGDHLVISIAAGV
                     KISFIESHLNWGVRVVRVMPNQPCFVGASASGFALGRSAKKEDGEIVQRIL"
     gene            759..1118
                     /gene="ndhC"
                     /locus_tag="PLJ11_02375"
     CDS             759..1118
                     /gene="ndhC"
                     /locus_tag="PLJ11_02375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOO03545.1"
                     /translation="MLVDDYFPVAIFALVALLVPVIAVVTGKLFRPTTRGAMRDTTYE
                     CGEKPIGEAQIQFHVQFYIYAIIFVAFDILTVFLLVWAMAFEQLSDIANIAAISFLII
                     MLAGVYYSLKKERILWI"
     gene            1109..1672
                     /gene="nuoB"
                     /locus_tag="PLJ11_02380"
     CDS             1109..1672
                     /gene="nuoB"
                     /locus_tag="PLJ11_02380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOO03546.1"
                     /translation="MDLDLSFNAMTMTVKQFGDMTKTFLREATVATGGKKVVDKLTTP
                     IWSWGQRNSVYPLHFGIACCALEMAAAGGPRFDLERLGIVFRSSPRQSDILLLNGWIS
                     AKLRPILRTLYEQMPAPKWVIAMGECTISGGPWYDAYNVIQGADTFIPVDVYVPGCPS
                     RPEALIQAFTMINRKLTAENRGSFLGD"
     gene            1682..2158
                     /locus_tag="PLJ11_02385"
     CDS             1682..2158
                     /locus_tag="PLJ11_02385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOO03547.1"
                     /translation="MSQVIVYDKPDRSEEEVASAIRAKFPSVELLKVAPRRLHFRAPR
                     ESLFELCKFMRDELAFEHASCVTPVDYLDHFTVVYHISSWTDHILAEVQVDLPRDDPT
                     VDSIAPLWGGANWHEREAYDLMGIVFKGHPDLRRIMLPEEFRFHPLRKDFIVGRRV"
     gene            2160..3245
                     /locus_tag="PLJ11_02390"
     CDS             2160..3245
                     /locus_tag="PLJ11_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoD"
                     /protein_id="HOO03548.1"
                     /translation="MTDEMWINMGPQHPFNHGLWNLRVKLKGETITAAEPIIGYLHRG
                     WEKMVENRAYPQIVPLADRLCYGSSLSWNHLYCLTAERLFGVEAPDRAKYIRVAALEM
                     QRITSHLTWLMAVGTDLGSYTIMTWSIRERELFLDLLCKLSGARMTYNYCRIGGVRND
                     LPENYERDVNRTLDEFDRRLRDLEALMDENEPFLMRTLGIGKMDGRTAMNLGLTGPIL
                     RASGVDMDLRKLDPYEAYADMKFEVCTHTDGDVYARYRVRMDEMRESMKIIRQALAEA
                     PAGPVRVKVPRCPPKGNVVCRVEDPRGESLMYMVSDGTERPYRLKVRSPNFVNLSASP
                     PMLVGFKISDVPAIMGVMDMCIGETDR"
     gene            3250..4335
                     /locus_tag="PLJ11_02395"
     CDS             3250..4335
                     /locus_tag="PLJ11_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOO03549.1"
                     /translation="MATLYTVLTDFTQWLMDLIAAILDWTKLGILSDLGDWLQSAPVV
                     DFVTTLLVAVIYLITVMMFALCFIWQERKFLGRFMDRRGTQVGVLGFFQNFADGIKVV
                     LKEHIVPKDSDHALFEMAIFTYLASTFMLFGCIPFSDNFYVIDVNLAVLLGIAIFAFG
                     PFSILVGGWASNNKYTLIGGMRAAAQLIALEIPLLLCVVSVVVLTGDLSFVGIVAWQE
                     ENIWLIFPLCIGALTFFIAAIGESERIPFDLPEAEAELVEGWATEYGDIRWGLQMASD
                     YFRAYILCALFTMLFLGGWAGPEFIWAEAWFLAKTFIVFFFFIWVRAATVRIRTDQIL
                     KLGWRRLLPLAVINLLIAVALKVAEVF"
     gene            4335..5090
                     /locus_tag="PLJ11_02400"
     CDS             4335..5090
                     /locus_tag="PLJ11_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO03550.1"
                     /translation="MRIEERTYTKADHLRPTGYVLKSLIVTFKGLVRATKNRPCTVLY
                     PWERLVYPDNYRGRPGIVLERCIGCARCARDCPNRCIEMVEVELPAGGKAKRPQVNVG
                     RCMMCGNCAETCPVNAMIVTPEFELCSYSRADLIFDPVKLQYPHKPGYEVNYEFALLS
                     EMKKGGEVRTYAKNDPNVHDNVVLDAPKCISCSRCAKTCPAGAIEMAETGELNPKTKK
                     PIKRPKFDTTKCVVCEQCIEVCPKSALSIKEVL"
     gene            5090..5395
                     /locus_tag="PLJ11_02405"
     CDS             5090..5395
                     /locus_tag="PLJ11_02405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOO03551.1"
                     /translation="MDGTLFFFIIFAALTVGCAMMVLISKDIVRSVVWLAATFLGVGI
                     LYLFLNAEYLFLIQILVYVGAINVLILFGIMLTKRRMGKEDACEEYEQNIVKRRRSP"
     gene            5392..5637
                     /locus_tag="PLJ11_02410"
     CDS             5392..5637
                     /locus_tag="PLJ11_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03552.1"
                     /translation="MRKGDSTQKIIAKVLIVGLLLELMLYSVLSLEWGDIGDTVLKTV
                     EIGTMLFKEYWVAVLMLGLMLFSVIIGGVFIAQEDDE"
     gene            5634..5936
                     /gene="nuoK"
                     /locus_tag="PLJ11_02415"
     CDS             5634..5936
                     /gene="nuoK"
                     /locus_tag="PLJ11_02415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOO03553.1"
                     /translation="MIPLEFVLIVSSALFAIGAAGVILKKNAIQVLMAIELMLNAANI
                     NLVAFSTYFGDAKGQGFALISIGIAAAEVTIGLAILLNLNKLRKTVDVDDINLLRW"
     gene            5939..7900
                     /gene="nuoL"
                     /locus_tag="PLJ11_02420"
     CDS             5939..7900
                     /gene="nuoL"
                     /locus_tag="PLJ11_02420"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOO03554.1"
                     /translation="MDILEYAWLIPVLPLLAFVIVGFLGSKMKDKGGIVSILGVGAGM
                     VIALWSAYELLTTGRIYRDSFEWVILSSDYSLEMGIFLDSVAAMMLIVVSFISFLVVI
                     YSTAYMHDEGPRRHRYFTEICLFVGVMLGLVLANNFFQMFVFWELVGLCSYLLIGFWF
                     ERPSAARAAKKAFLVTRVGDVMFMIGLFILFTELHTLNYESVWAALDTADPAMMAFAA
                     FFLFGGAIGKSAQFPLHDWLPDAMEGPTTVSALIHAATMVNAGVYLVARAFPIIATSA
                     EVSLFVGAIGGITAFLAATMALCAPKIKGVLAYSTVSQLGYMILGLGAGAFLWNKGYH
                     IEASIAFGAGLFHLMNHAFFKGQLFLGAGSVIHYVDTEELSRMGGLGKYMKVTSITML
                     ISCLSIAGIPPLSGFWSKDEVLSVTFDAGDAGLTFMVLWVLGVLTAFMTAFYMFRMWF
                     MVFKGQPREGSKQAIEHGHHHEAPIAMLAPLVILAALAFGSGFSLFIGDGFFGAIYYE
                     HAHVLSIGERLEEVFTSPLTYVSIVAAVGGIMLAYYSFYKPKISAEKVVAKGFPKAMH
                     QLLLDRYKFPVAYDKIGYAGLYGFSLLLDKFDRYIIDGIVNGISAFLIRAGGTVRKLQ
                     NGFVKSYATLLLFGVSVVLILLYVVGALR"
     gene            7905..>9188
                     /locus_tag="PLJ11_02425"
     CDS             7905..>9188
                     /locus_tag="PLJ11_02425"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOO03555.1"
                     /translation="MELPILSLLLLFPLIGAVITFMMGKNPKLAKISALVFSAIPLVL
                     SLLMLTEYVQTAGGYQFKESFTWIESLGISYILGVDGISVPMVFLTTLLVFLAIIFSW
                     DIDHRTNQYMGLMLVLELGVLGVFMALDYFLFYIFWEVVLIPMYFLINVWGGPRRGYA
                     AIKFFIYTHVASLVMLLGIFALYFEAASVNGFNSFSMETINAVSPFFGNTFQVIVFGA
                     LFFGFAVKMPSFPFHTWLPDAHVEAPTGGSVLLAGLLLKMGSYGVIRIAFPALPDGAH
                     EWQWVMVALAILSIVYGAIMCLAQKDLKKMVAYSSISHMGIVMLGFATFTELGIAAGV
                     LQMFAHGLITAVLFMMCGVVQHKTGTRNIPRLGGLAAKMPFAATFMMIGFLASLGLPG
                     MVGFVAEFSVFTATFDAFGWLLLIPITSVAITAGYY"
     assembly_gap    9189..9198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9199..9381
                     /locus_tag="PLJ11_02430"
     CDS             <9199..9381
                     /locus_tag="PLJ11_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOO03556.1"
                     /translation="LFGPLTKEVDVEHLHDVYWFEALPLAVLSGFIVLFGMFPALVLD
                     YIRPALGAVIGLLGGI"
BASE COUNT         1748 a         2902 c         2852 g         2046 t
ORIGIN      
        1 aggatcctct gcacgatctc cccatcctcc ttcttggccg agcggccaag ggcgaacccc
       61 gatgcggagg cccctacaaa gcagggctgg ttgggcatta cccggaccac cctcacgccc
      121 caattcagat gggattcaat gaaggatatc ttgaccccag cggcgatgga tatcacaagg
      181 tggtcgccgc tgagataggg tttgagctca tccagcaccg ggccgacctg ttgcggcttc
      241 accgcaagga tgatcacatc ggaggagcgc acgacctcta tggcgtcgga gacggtccgt
      301 acgcccaggg tcctggtgat gtagtccctg cgctggggca cgatctcgct ggcgatcatg
      361 tcctccttcg ttgccactcc agccgatatt atccccttca tcatggcctc ggccatgttc
      421 cccgcgccta cgaacccgat cctcatattt tcacccatgt tcggatgacc gtccatcgaa
      481 tccttcgtat taatccgatt tggtaggcct ccccgaatgc aacgccccct tgcgccgggt
      541 gatggttgga gcgggaattc ctcctagctc ggccccgctc gtccccgggg gtggggcaag
      601 gacatcgatg gcgcgtatgt ctagtaccgg acataggcaa gatttttata atttatatta
      661 ttgttaatat ataaccaact ggtcatgcta aaaaaatctt cattatcgcc ccccaacatt
      721 ctcaaaagta taaataaagg gaccattatc ccccttcaat gctcgtagac gactatttcc
      781 cggttgcaat atttgctctg gtagctttgc tcgtccccgt catcgccgtt gtcacgggga
      841 agctattccg gccgaccacg cggggggcga tgagagatac cacctacgag tgcggggaga
      901 agcccatagg agaggcccag atccagttcc atgttcaatt ctacatatat gcaataatct
      961 tcgtggcctt cgacatcctt accgtttttc tcctcgtctg ggccatggcc tttgagcagc
     1021 tctccgacat cgcgaacatt gccgcgatat cgttcctgat catcatgctc gccggggtct
     1081 actattcatt gaagaaggag aggatattgt ggatctagac ctgtcgttca acgccatgac
     1141 catgacggtc aagcagttcg gcgatatgac gaagacgttc ctgcgcgaag cgacggtggc
     1201 cacggggggc aagaaggtcg tcgataagct taccaccccc atatggtcat gggggcagag
     1261 gaactcggtc tatcctctgc acttcgggat cgcctgctgc gccctggaga tggccgcggc
     1321 cggtggcccc cggttcgacc ttgagcggct cgggatcgtg ttccggtcct cgccaaggca
     1381 gtctgacata ctccttttga acggatggat atctgcaaag ctccgtccca tcctgagaac
     1441 cctttacgag cagatgcccg cgcccaagtg ggtcatcgca atgggcgaat gcaccatctc
     1501 cggcgggccc tggtatgacg catacaacgt catccagggc gcggacacgt tcatccccgt
     1561 cgacgtgtac gtccccggat gcccctcccg ccccgaggcc ctgatccagg cctttaccat
     1621 gatcaaccga aagttgaccg ccgagaaccg tggcagcttc ctaggcgatt aaggtggtat
     1681 aatgagccaa gtcatcgttt acgacaagcc cgaccggtcc gaggaagagg tcgccagcgc
     1741 catacgggcc aagttcccct ccgtggaact cctcaaggtc gccccgcgca ggctgcactt
     1801 ccgagcgccc cgcgaatcgc tcttcgagct ctgcaagttc atgcgggacg aactggcatt
     1861 cgagcacgcc tcctgtgtga ccccggtcga ctacctggac catttcacgg tcgtctatca
     1921 catctcctca tggaccgatc acatcctcgc cgaggtccag gtggatttgc ctcgggacga
     1981 tccgaccgtg gactccatcg cccccctctg ggggggtgcc aattggcatg agcgggaggc
     2041 ctatgacctt atggggatcg tcttcaaggg gcatcccgac ctccgccgca tcatgcttcc
     2101 ggaggagttc aggttccatc ctttgaggaa ggacttcatc gttggcagga gggtctgaga
     2161 tgacggacga gatgtggatc aacatggggc cgcagcaccc attcaaccac ggcctgtgga
     2221 acctcagggt gaagctcaaa ggggagacga tcacggccgc cgagccgatc atcgggtacc
     2281 tgcacagggg ctgggaaaag atggtggaga accgcgccta tccccagatc gtcccgctcg
     2341 cggaccgcct ctgctacggc tcgtcccttt cctggaacca cctttactgc ctgacggccg
     2401 agaggctgtt cggggtcgag gcccctgacc gggccaaata catccgcgtg gccgcattgg
     2461 agatgcagcg catcacctcc cacctcacct ggctcatggc cgtcgggacc gacctgggaa
     2521 gctacaccat catgacatgg tcgatcaggg agagggagct gttcctggac ctcctgtgca
     2581 aactgagcgg cgcgcgcatg acctataact attgtcgcat cgggggggtc aggaacgacc
     2641 ttccggagaa ctatgagcgc gatgtgaacc gtaccctgga tgagttcgac aggcgcctcc
     2701 gtgacctcga ggccctgatg gatgagaacg agccgttcct gatgaggaca ctgggcatcg
     2761 ggaagatgga cggcaggacc gcgatgaacc tcgggctgac agggccgatc ctgagggcaa
     2821 gcggcgtcga catggacctg cggaagctgg acccgtacga ggcctatgcg gatatgaagt
     2881 tcgaggtatg cacccacacc gatggggatg tatacgcccg gtaccgggtg cgcatggacg
     2941 agatgcggga gagcatgaag atcataaggc aggcgttggc ggaggccccg gccggcccgg
     3001 tccgtgtcaa ggtcccgcga tgcccgccga aggggaacgt ggtctgtcgc gtggaggacc
     3061 cgcgcgggga atcgttgatg tacatggtga gcgacggcac ggagaggccg taccgcctca
     3121 aggtccgctc cccgaacttc gtgaacctgt ccgcctcccc gccgatgctg gtcggcttca
     3181 agatctcgga cgtcccggcc atcatgggcg tcatggacat gtgcatagga gaaacggacc
     3241 ggtgatcaaa tggctaccct ctataccgta ttgaccgatt tcacccagtg gctgatggac
     3301 ctgatcgctg cgatcctcga ttggaccaag ctcgggatcc tcagcgacct cggcgactgg
     3361 ctccaatccg cccccgtcgt cgatttcgtc acgacgctcc tggtggccgt gatctacctg
     3421 atcaccgtca tgatgttcgc cctatgcttc atatggcaag agcggaagtt cctcgggagg
     3481 ttcatggacc ggcgcggtac ccaggtgggg gtgctcgggt tcttccagaa cttcgcggac
     3541 gggatcaagg tcgttctgaa ggagcacatc gtccccaagg actccgatca cgccctcttc
     3601 gagatggcca tcttcaccta tctcgcctcg accttcatgc tcttcgggtg cattcccttc
     3661 tctgacaact tctatgtgat cgatgtcaac cttgcggtgc tcttggggat cgccatcttc
     3721 gccttcggcc cgttcagcat cctggtcggt ggatgggcct cgaacaacaa gtacaccttg
     3781 atcggcggca tgagggctgc cgcccagctc atcgccctcg agatcccgct cctgctctgc
     3841 gtcgtctccg tggtggtatt gacgggtgac ctctctttcg tgggaatagt ggcctggcag
     3901 gaggagaaca tctggctcat attcccgctg tgcatcggcg ccctcacctt cttcattgcg
     3961 gctataggag agtccgagcg catcccgttc gacctccccg aggccgaggc ggagctggtc
     4021 gaagggtggg ccactgaata tggggatata cgctggggcc tgcagatggc ctccgactac
     4081 ttccgcgcct acatcctctg cgcgctgttc accatgctct tcctgggggg atgggccggt
     4141 cccgagttca tctgggcgga ggcctggttc ctggcgaaga ccttcatcgt gttcttcttc
     4201 ttcatctggg tgagggcggc caccgttagg atcaggaccg atcagatcct gaagctcgga
     4261 tggaggcggc tcctgcccct cgcggtgatc aacctgctaa tagccgtcgc cctcaaggtc
     4321 gcggaggtgt tctgatgagg atcgaagaaa ggacctacac aaaggcggac cacctcaggc
     4381 ccacgggcta cgtgctcaag tcccttatcg tcacgttcaa gggccttgtg cgggcaacga
     4441 agaacaggcc gtgcacggtc ctgtacccct gggagaggct ggtatacccc gacaactacc
     4501 ggggacggcc gggcatagtc ctggagagat gcataggctg cgcccgctgc gccagggact
     4561 gccccaaccg ctgcatcgag atggtcgagg tcgagcttcc cgcggggggc aaggcgaagc
     4621 gtccgcaggt caatgtcggc cggtgcatga tgtgcggcaa ttgcgccgag acgtgcccgg
     4681 tcaatgccat gatcgtcacc ccagagttcg aactctgctc gtatagcagg gcggacctca
     4741 tcttcgatcc ggtgaagctc cagtaccccc acaagccggg ctacgaggtg aactatgagt
     4801 tcgccctgct gtccgagatg aagaagggcg gggaggtgag gacgtacgcc aagaacgatc
     4861 ccaacgtcca tgacaacgtg gtcctggacg cccccaagtg catctcgtgc tcgaggtgcg
     4921 cgaagacctg cccggcggga gcgatcgaga tggcggagac tggggagctg aaccccaaga
     4981 ccaagaagcc gatcaagcgc cccaagttcg acaccaccaa gtgcgtggtg tgcgagcaat
     5041 gcatcgaggt atgccccaaa tcggcgctaa gcatcaagga ggtgttgtga tggacggaac
     5101 cctgttcttc ttcataatat tcgccgcgct caccgtggga tgtgccatga tggtgctcat
     5161 ctccaaggac atagtgcgga gcgtggtgtg gcttgcggcc accttcctcg gcgtgggaat
     5221 actctacctg ttcctgaacg cggagtacct gttccttatc cagatcctcg tgtacgttgg
     5281 cgccatcaat gtgctcatcc tcttcgggat catgctcacc aagcgccgga tgggcaagga
     5341 ggacgcttgc gaggagtacg agcagaatat cgtcaagagg aggcggtccc catgaggaag
     5401 ggcgattcca cacagaagat catagccaaa gtgctgatcg tcgggctgct tctcgagctc
     5461 atgctgtact cggtgctctc cctggaatgg ggcgacatcg gcgacacggt gctgaagacc
     5521 gtggagatcg ggaccatgct gttcaaggag tactgggtgg cggtgctcat gctgggcctg
     5581 atgctcttct ccgtcatcat tggcggcgtg ttcattgcac aggaggacga cgaatgatcc
     5641 cgctggagtt cgtgctcatc gtatcatcgg ccctgttcgc catcggggcg gccggggtca
     5701 tcctgaagaa gaacgccatc caggtgctga tggccatcga gctcatgctc aacgcggcca
     5761 acatcaacct ggtggccttc tcgacctatt tcggggatgc caaggggcag ggcttcgccc
     5821 tgatatccat cggcatcgcc gcggccgagg tgaccatagg gctcgcgatc ctgctaaacc
     5881 tcaacaagct gaggaagacc gtggacgtgg acgacattaa cctgctgagg tggtaaagat
     5941 ggacatactg gaatatgcct ggttgatccc ggtcctgccg ttgctcgcct tcgtcatcgt
     6001 gggcttcctg ggtagcaaga tgaaggacaa gggcggcatc gtctccatcc tcggggtcgg
     6061 ggctggaatg gtcatcgcgc tgtggtccgc gtacgaactg ctcaccaccg gccggatcta
     6121 tagggactcc ttcgaatggg tcatcctcag ctccgactac tccctggaga tggggatctt
     6181 cctggacagc gtggcggcca tgatgttgat agtggtctcc ttcatctcct tcctggtggt
     6241 catctactcc accgcctaca tgcacgatga ggggccgagg cgccaccggt acttcaccga
     6301 gatctgcctg ttcgtaggcg tcatgctcgg cctggtgctg gccaacaact tcttccagat
     6361 gttcgtattc tgggagctcg taggcctctg ctcctacctg ctgatagggt tctggttcga
     6421 gaggccctcc gcggcccgcg cggccaagaa ggccttcctg gtcaccaggg tgggggacgt
     6481 catgttcatg atcggcctct tcatcctgtt caccgagctg cacacgctga actacgagag
     6541 cgtgtgggct gcgctcgaca ccgccgaccc ggccatgatg gccttcgcag ccttcttcct
     6601 gttcggcgga gcgataggca agagcgccca gttcccgttg cacgactggt tgccggacgc
     6661 gatggagggt ccgaccacgg tgtccgccct gatccatgcg gccaccatgg tcaacgccgg
     6721 tgtgtacctg gtcgcaaggg ccttcccgat catcgccacg agcgcggagg tcagcctttt
     6781 cgtcggcgcc atagggggca tcaccgcctt cctcgcggcc acgatggccc tttgcgcgcc
     6841 gaagatcaag ggggtcctcg cctactccac cgtctcccag ctgggctaca tgatccttgg
     6901 gctgggtgcc ggggcgttcc tatggaacaa aggctatcat atcgaggcgt cgatcgcctt
     6961 cggggccggc ctgttccacc tgatgaacca tgccttcttc aaggggcagc tcttcctagg
     7021 cgcagggtcg gtcatccact acgtggacac ggaggagctg agcaggatgg gcgggctcgg
     7081 caagtacatg aaggtgacca gcatcaccat gctgatctcg tgcctgtcca tcgctggcat
     7141 cccgcccctc tccggcttct ggagcaagga cgaggtgctc tccgtgacgt tcgacgccgg
     7201 ggacgccggc ctgaccttca tggtcctctg ggtgctcggg gtgctgacgg ccttcatgac
     7261 cgccttctac atgttccgca tgtggttcat ggtgttcaag ggccagccca gggagggcag
     7321 caaacaggcc atcgaacacg ggcatcatca tgaggcgccg atcgccatgc tcgccccatt
     7381 ggtcatcctg gccgcacttg ccttcgggtc aggcttctcc ctcttcatag gggacgggtt
     7441 cttcggggca atctactatg agcacgcgca cgtgctgagc ataggcgagc gcctggagga
     7501 ggtgttcacc tccccgctga cctatgtatc gatagtggcc gcggtgggag gcataatgct
     7561 ggcctactac agcttctaca agcctaagat ttcagccgag aaggtcgtgg caaagggctt
     7621 ccccaaggcc atgcaccagc tgctgctgga ccgctacaag ttccctgtgg cctatgacaa
     7681 gataggctat gcaggcctgt acggcttctc cctgctgctc gacaagttcg atcgctatat
     7741 catagacggc attgtgaacg gcatcagcgc cttcctgatc agggcgggcg ggacagtgcg
     7801 caagctccag aacggcttcg taaagagcta tgcgaccctc ctcctgttcg gggtatcggt
     7861 ggtcctgatc cttctctatg tcgtaggagc gctgaggtga tcccatggaa ctaccgatac
     7921 tatccctact gttgctcttc ccgctgatcg gtgctgtgat caccttcatg atgggaaaga
     7981 accccaagct ggccaagatc tctgcgctgg tgttctccgc gataccgctg gtgctgtccc
     8041 tgctcatgct caccgagtat gtgcagaccg cgggcggcta tcagttcaag gaatctttca
     8101 cctggatcga gtccctcggg atctcgtaca tcctcggcgt ggacgggatc agcgtcccga
     8161 tggtcttcct caccactctg ttggtgttcc tggcgatcat cttctcctgg gacatcgatc
     8221 accgcaccaa ccagtacatg gggctgatgc tggtgctgga gctgggggtg ctgggcgtct
     8281 tcatggccct ggactacttc ctgttctaca tcttctggga ggtcgtgctg atcccgatgt
     8341 acttcctgat caacgtgtgg ggcggccccc gcagggggta cgcggcgatc aagttcttca
     8401 tatacaccca cgtcgccagc ctcgtcatgc tgctggggat attcgccctc tacttcgagg
     8461 cggcctccgt gaacgggttc aactccttca gcatggagac gatcaacgcc gtgtccccct
     8521 tcttcgggaa tacgttccag gtgatcgtgt tcggcgccct cttcttcggc ttcgccgtga
     8581 agatgccgtc gttcccgttc catacgtggc ttccggacgc gcatgtcgag gcgcccaccg
     8641 gggggtcggt cctgctggcc gggctgctgc ttaagatggg ctcctacggg gtcatccgca
     8701 tcgccttccc ggccctgccc gatggcgccc acgaatggca atgggtcatg gtcgccctcg
     8761 ccatcctgtc catagtctat ggggccataa tgtgcctggc gcagaaggac ctcaagaaga
     8821 tggtggccta ctcctccatc agccacatgg gcatagtgat gctcgggttc gccacgttca
     8881 cggagctggg catcgccgca ggcgtgctcc agatgttcgc ccacggcctc atcaccgccg
     8941 tcctcttcat gatgtgtggg gtggtgcagc ataagacggg caccaggaac atcccccggc
     9001 tgggtgggct cgcggccaag atgccgttcg ctgccacgtt catgatgatc gggttcctgg
     9061 catccctggg ccttcccggg atggtcgggt tcgtggccga attctcggtc ttcaccgcga
     9121 ccttcgatgc cttcggttgg ctgctgctga tccccatcac aagcgtggcc atcaccgccg
     9181 gctactacnn nnnnnnnnct gttcggcccg ctgaccaagg aggtggacgt cgagcacctg
     9241 catgacgtgt actggttcga ggcgctgccg ctggccgtgc tctccgggtt catcgtgctc
     9301 ttcggcatgt tcccggccct ggtccttgac tacatccggc ctgcgctcgg cgctgtgatc
     9361 ggtctactgg gagggatcta agtggactac tcagcgatct atcccgagct gatattggcg
     9421 ctgttcgcca tcgccgttcc cgcggtcaac atcctcgtga aggacagcag gttccttgcg
     9481 gggttctccc tcgcgggggt gggtctgtcc atgctggcag tggtcatgca cttcgtcgac
     9541 ggcacgggca gggtggta
//
LOCUS       DAOSYN010000030         9514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_6149_length_9514_cov_4.791627, whole genome shotgun sequence.
ACCESSION   DAOSYN010000030 DAOSYN010000000
VERSION     DAOSYN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6149_length_9514_cov_4.791627"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLJ11_02435"
     CDS             <1..420
                     /locus_tag="PLJ11_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03557.1"
                     /translation="ALRLILRFEVPGLRPYEAQRPCAPVLFAVAVTIAFLLFPMGVSL
                     PVIAAMGWVDPLIGELRRRRSDAYPLLPLLAYSATLLVGLALFYGPTFPVLLSAVLVS
                     PIALFLEAQRFRLLDDDLVLVLVPLLLVALVLGQLPW"
     gene            complement(428..574)
                     /locus_tag="PLJ11_02440"
     CDS             complement(428..574)
                     /locus_tag="PLJ11_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03558.1"
                     /translation="MSKFIYQCLVCDKVHKTLEAAENCHKGPTQTIEQGLTGFSKRKG
                     IIGN"
     gene            769..1266
                     /locus_tag="PLJ11_02445"
     CDS             769..1266
                     /locus_tag="PLJ11_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOO03559.1"
                     /translation="MNKIKVINEPSELVSMLRAVDTPVKRDVLKEVTLEWRTVREIEE
                     RYGTEGKEALTFFEKMKLVETRWQPTEGGRPEKAYHTYYNSFHINAQWPVYEISDVLY
                     AAMMPEDEFKEVEENILEQIGKEGRFAGDVAEKLGMTATLLKSLVKRSVKMDYRGHRL
                     ERVKE"
     gene            1268..1789
                     /locus_tag="PLJ11_02450"
     CDS             1268..1789
                     /locus_tag="PLJ11_02450"
                     /EC_number="2.1.1.206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HOO03560.1"
                     /translation="MPEITVLRLGHRPERDKRITTHVELTARAFGARRIVVSTKDAGL
                     EESVRDVVRRFGGDFEITTGVNWRRFLEEFQGTVVHLTMYGLPVDEVAPKVPDGNVLV
                     VVGAEKVPPEVYQRADLNVAVGNQPHSEVAALAILLDRVFHGRMLQRDYEGAQIKVLP
                     CERGKNVVELSKQ"
     gene            1803..2339
                     /locus_tag="PLJ11_02455"
     CDS             1803..2339
                     /locus_tag="PLJ11_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOO03561.1"
                     /translation="MLREEGVPPAVIRHSCVVNLVAMELAKRCGADLGLVNAASLLHD
                     IGRSRTHGIQHVTEGVRIARERGLPEDLVRCIGRHIAAGFTPEEAEGLGLPEGDYMPV
                     TLEDKLVNLADNLVSDRSIKTLAQAAERMRSKGFELTAVRMLAMGEEIARLCGEGIDP
                     ILEKAGVREAAARCDRVP"
     gene            2466..3395
                     /locus_tag="PLJ11_02460"
     CDS             2466..3395
                     /locus_tag="PLJ11_02460"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOO03562.1"
                     /translation="MSERKTMMWMNGRLIEQEKALLPFMTHALHYGSSVFEGIRIYDT
                     PKGKAVFRLREHMVRFLNSAKVMCMAHPYTIEELIEATKLAVRHASPDSDYVRPLMWY
                     GENDENRIVINPFKFKVNVAIACAHMGAYLGKENMEKGAKLITSSWTKMANTSTSLQA
                     KIGGNYVNSILAKIEATQLGADECLMLNTNGTVAEGPGENIFMVKNGKLFTPPLASGV
                     LEGITKDSVVTIAQDKGYQVIEKEITRSEVYMCDEFFMTGTAAEITPVRSLDERTIGD
                     GRRGPITKVLQEAFFEAARGNDPRYVHWLTPVA"
     gene            3463..4365
                     /locus_tag="PLJ11_02465"
     CDS             3463..4365
                     /locus_tag="PLJ11_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO03563.1"
                     /translation="MYEIRFHGRGGQGAVMAAQTIAEAAILEGRQAQAFPFFGAERRG
                     APVMAFARIDDRRICDRTQVAEPDMLVVMDASLLDIEPVALGLKKEGCAVVNSPMRPE
                     EIDLGVVARCVSVDATSIALEHLKAPIVNTAILGAMARADAFVSLASIQRAIVNRFGE
                     KLGERAGSINSEAAGAAFERSREGMCRGERKMQRGNAWLPTWQETPPGVALGRWERGG
                     MAVGPGSMRQNMTGTWGTSSPTYTRARCIGCQRCWFICPEGCIRRLEDDSLEFDLRYC
                     KGCGLCAKVCPKEAIAMHRKGAGA"
     gene            4362..5537
                     /locus_tag="PLJ11_02470"
     CDS             4362..5537
                     /locus_tag="PLJ11_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOO03564.1"
                     /translation="MRAVRALTGNAAAAYGVKLCRPEVIPNYPITPQTIIIEQLADFI
                     NDGELDADFIPADSEHSAMSIAIGAAATGVRTFTATASHGLALMHEMLYAPPQLRLPI
                     LLVDVNRSLGAGSGIWVEYNDSMPERDSGWMQAYAEDAQEVLDMVIQAYRIAEDPRVV
                     LPFMLCLDGFILSHTVERVEVPGQEEVDAFLPRFSPLNRLDPEAPMCMNMAVSPTHAM
                     EMRYQLDRQMEGARQVIAEVDEAFGKAFGRSYGGLLDTYLMEDAEVALITLGTATSTA
                     RVAVDELRAEGRKVGLIKLRFMRPFPHEELREAVKGLRALGVFDRSVSFNRFGPVFTE
                     VRSSLYGSGVPITDHIAGIGGRDLKVSTFRDMFALLERSASGGRVRECTWHGLRGEM"
     gene            5537..6490
                     /locus_tag="PLJ11_02475"
     CDS             5537..6490
                     /locus_tag="PLJ11_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO03565.1"
                     /translation="MEQRVNIRSMPREDHLLSGHGACAGCGVAIAVKNLMRVLGRNTT
                     VYVPASCLLIFGGGMYPHSAFDVPFLYSAFENTGAVISGIKAGYRRQGKDVTVVGIAG
                     DGGTFDIGLQALSGAAERNEDVIYVCVDNEAYMNTGTQRSGSTPFGAWTTTTPVGSRV
                     KGKRSFKKDLMAVVASQDIAYAATLSIAHMNDFVQKVERAKAMKGFRFLHIMAPCPSG
                     WKADPSLTYDLARLAVETGMWSLYEVEEGEAHVTYRPKEMLPVGEYLKLQGRFKHMDR
                     EDVEALQAWLCRKWRMRYAPSQPPLDRTDDLACDGDGCEGA"
     gene            complement(6513..8105)
                     /locus_tag="PLJ11_02480"
     CDS             complement(6513..8105)
                     /locus_tag="PLJ11_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOO03566.1"
                     /translation="MVDQTLFWAATAGYAVVLLYLAWLGYRRTKCNADYMLAGGNVHP
                     VIIGLSYGATFISTSAIVGFGGWAAKLGLGIIWLVLLNILVGVLIAFIVFGKPTRRIG
                     KRLGALTFPDLLGKVYRSNFMQYATGLVILIGMPLYSSAVLIGGGRFIEMVLDVPYLW
                     ALIGLTAVTAAYVVFGGLMAVMYTDALQGALMLVGMSAIMVLTLGAVGGLDGFQALTD
                     MAPLVPADLASAGMVGWTAFPEVGSSTWYTLVTTIIMGVGIGVLAQPQLAVRFMTAGD
                     GRSLNRAVPVGAVFILITTGAAYTMGALTNVYFYEHFGKISTEMTGNIDNIVPLFIDM
                     AMPGWLVVMFALVLLAAAMSTMSSLFHSMGTAAGHDIWVHLRRSKLLLERAHGGDEDA
                     SKLRVNKLATFVMIAVSLGLALIMPFDIIARATAMFMGLCAAAFLPAYAMAVFSERPS
                     ALGAKASLVMGSVTWGLWTVFVHRAEAEVLGIAGLLTGRASLLAYPWDMVDALFIALP
                     LSVAALAIGTLVERRRGSATGA"
     gene            complement(8323..>9514)
                     /locus_tag="PLJ11_02485"
     CDS             complement(8323..>9514)
                     /locus_tag="PLJ11_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOO03567.1"
                     /translation="YRQRMREAGVTPEDVQGLDDVRRLPFMTKRDLRDSYPDKLFLAS
                     PREVVRYHASSGTTGKPTIVGYTRNDIDSWSESVARGLVSAGVGPDDVLQVCYTYGLF
                     SGGLGLHYGAEKVGAAVVPASTGNTERQIDLIRDLNVTVIASTPSYLLHIGEVAERMG
                     VSIERDTQLRLGILGAEPWSDRMRDRIHERLGIKAVDIYGTSELSGPLWCECSEQKGM
                     HVWGDMTLVEVLDPATGEPVPPGEKGELVVTMLEKEAFPIVRYRTGDVTTLTEEVCAC
                     GRTHPRIGRIQGRVDDMIVVRGINVFPSQVEHALMTDPEVGNEFQIVVERRGALDTML
                     VKVELRPEAFGDRLFELDAIKDRLAHRLRDSLNVGVSVEIVEPGSLPRFEGKAKRVID
                     KRVY"
BASE COUNT         1761 a         3015 c         3153 g         1585 t
ORIGIN      
        1 gcgctccgcc tcattctcag gttcgaggtc ccggggcttc ggccctacga ggcccagcgc
       61 ccctgcgccc ccgtgctctt cgcggtggcg gtcaccatcg ccttcctcct cttcccgatg
      121 ggggtttccc tgcccgtcat cgcagccatg ggctgggtgg acccgctgat cggggagctt
      181 cgtcgccgcc ggagcgatgc ctatccgctg ctccccctgc tcgcctattc cgcgacacta
      241 ctggtcggcc tggcgctctt ctacggcccg acgttcccgg tcctgctctc cgcggtcctg
      301 gtgtccccca tcgccctgtt cctggaggcg cagcggttcc gccttctcga cgacgacctg
      361 gtgctggtcc tggtaccgct cctgctcgtg gcgctggtcc tgggccaact gccttggtag
      421 aaaaagctca gttgccgatg atgcccttgc gcttgctgaa gcccgtcagg ccctgctcga
      481 tggtctgggt aggtcccttg tggcagttct ccgccgcctc aagggtcttg tggaccttgt
      541 cgcacactag gcattgatag atgaacttgg acatcgtgga atgaaatcga tagggtgctt
      601 ataatcctat tgcctccccc tagccctcag tctcaccgcc cgttcggcct tccttggaaa
      661 aggtgccccc cggccctggc caacgtccta taggggattc aaaacggaat ttcgtttatt
      721 taggtgattc cgcagggttt ttatttcctg aaagcaatta gtagaggtat gaacaagatc
      781 aaggtcatca acgaaccctc tgaactggta tctatgctga gggcggtgga tacccctgtc
      841 aagagggacg tgctgaagga ggtgaccttg gaatggcgca ccgtgaggga aattgaggag
      901 aggtacggaa ccgaaggcaa ggaggccctg accttcttcg agaagatgaa gctggtggag
      961 accagatggc agcctaccga gggcggccgt cctgagaagg cctatcatac ctattataat
     1021 tcgttccaca tcaacgctca atggccagtg tacgagatct cagacgtcct gtacgccgcc
     1081 atgatgcccg aggacgagtt caaggaggtg gaggagaaca tcctcgagca gatcggcaag
     1141 gaaggcaggt tcgccggcga tgtggccgag aagctcggca tgaccgccac cttgctcaag
     1201 agccttgtga agcgctcggt caagatggac taccgcgggc accgcctgga aagggtgaag
     1261 gagtaaggtg cccgagatca ccgtgctccg cctcggccac aggccggaga gggacaagag
     1321 gatcacgacg cacgtggagc tcaccgcccg ggccttcggg gcccgccgga tcgtcgtctc
     1381 caccaaggac gccgggctcg aggagagcgt cagggatgtg gtgaggcgct tcggcgggga
     1441 cttcgagatc accaccgggg tgaactggcg caggttcctg gaggagttcc agggaacggt
     1501 cgtgcaccta accatgtacg ggctgccggt ggacgaggtc gcgccgaagg tccccgacgg
     1561 gaacgtcctc gtggtcgtcg gggcggagaa ggttcccccg gaggtctacc agagggcgga
     1621 cttgaacgtg gcggtgggca accagccgca ttccgaggtg gccgccctgg cgatcctgct
     1681 ggaccgggtg ttccacgggc ggatgctgca gagggactac gagggggccc agatcaaggt
     1741 gctcccctgc gagaggggga agaacgttgt cgagctatcc aagcagtgag gagtgctacc
     1801 gtatgctgag ggaggagggc gtccctccgg ccgtgattcg tcattcctgc gtcgtgaacc
     1861 tcgtcgcaat ggagctcgcc aagcgatgcg gggcggacct ggggctggtg aacgccgcct
     1921 ccctgctgca tgacatcggc cgctcgcgca cccacgggat acagcacgtc accgagggcg
     1981 tgcgcatagc gcgggagcgc ggcctgcccg aggacctggt gaggtgcata gggcgccaca
     2041 tcgcggccgg cttcacgccg gaggaggccg aggggctcgg ccttccagag ggcgactaca
     2101 tgccggtgac cctggaggac aagctcgtga acctcgccga caatctggtg agcgaccggt
     2161 ccatcaagac cctggcccag gcagccgagc gcatgcgcag caaagggttc gagctcaccg
     2221 cggtccgcat gcttgccatg ggggaggaga tagccaggct gtgcggggag ggcatcgatc
     2281 ccattctgga gaaggcaggg gtccgggagg ccgcggccag gtgcgacagg gtcccctgac
     2341 ccccgtaccg gttttcaacc cccgtattgg ttttcaaaaa ggttttaagc gtgccacttc
     2401 ttaccaagcc gcacgccccc ggctcatcgg ccaaatgggg cgcacgcgtc cttaggtgat
     2461 ccagcatgtc agaaaggaag accatgatgt ggatgaacgg caggttgatc gaacaggaga
     2521 aggcgcttct gcccttcatg acccacgcac tgcactacgg cagcagcgtg ttcgagggca
     2581 tccgcatcta tgacaccccc aagggcaagg cggtcttccg cctccgcgag cacatggtgc
     2641 gcttcctcaa ctcggccaag gtcatgtgca tggcccaccc atacacgatc gaggagctca
     2701 tcgaggcgac caagctcgcg gtgcgccatg cctcgcccga ctccgattac gtccggccgc
     2761 ttatgtggta cggggagaac gacgagaacc gcatcgtcat caacccgttc aagttcaagg
     2821 tgaacgtggc gatcgcctgc gcccacatgg gcgcgtacct cgggaaggag aacatggaga
     2881 agggggcgaa gctcatcacg tcctcctgga ccaagatggc caacacctcg acctcgctgc
     2941 aggccaagat cggcgggaac tacgtgaact ccatccttgc caagatcgag gccacccagc
     3001 ttggggccga tgagtgcctg atgctcaaca ccaacggcac cgtggccgag gggccagggg
     3061 agaacatctt catggtcaag aacggcaaac tgttcacgcc cccgttggcc tcgggcgtgc
     3121 tggagggaat caccaaggac tcggtggtca ccatagccca ggacaaggga taccaggtca
     3181 tagagaagga gatcacccgc tccgaggtat acatgtgcga cgagttcttc atgaccggaa
     3241 cagccgcgga gataaccccg gtccgctcgc tggacgagag gaccatcggg gacgggcgga
     3301 ggggaccgat caccaaggtc ctgcaggagg ccttcttcga ggccgcccgc gggaacgatc
     3361 cccggtacgt gcactggttg accccggtgg cctgagggcc gtacgccttg cagttctcaa
     3421 agggcggtcc gatggttccg gcgatgttcc tgggggcgta gcgtgtacga gatcaggttc
     3481 catggcaggg gcgggcaggg agcggtcatg gccgctcaga ccatagccga ggcggccatc
     3541 ctcgagggga ggcaggccca ggcgttccca ttcttcgggg cggagcgccg gggagcgccg
     3601 gtgatggcct tcgcgcgcat cgacgacagg aggatatgcg acaggaccca ggtggcggag
     3661 ccggatatgc tggtggtcat ggacgcgtcc ctgctggaca tcgagccggt ggccctcggc
     3721 cttaaaaagg agggatgcgc ggtcgtgaac agccccatgc ggccggagga gatcgacctg
     3781 ggggtggtgg caagatgcgt gagcgtggac gccacgagca tcgcgctgga gcacctgaag
     3841 gcacctatcg tcaacacggc catcctcggg gccatggccc gcgcggacgc cttcgtgtca
     3901 ctggcttcca tccaaagggc gatcgtgaac cgcttcggcg agaagctcgg cgagagggcg
     3961 gggagcatca actccgaggc ggccggggcc gcgttcgaac ggtcccgcga ggggatgtgc
     4021 aggggggaga ggaagatgca gcggggtaat gcatggctgc cgacctggca ggagacccct
     4081 cccggcgtcg ccctggggag gtgggagaga ggtggcatgg ccgtgggccc gggcagcatg
     4141 aggcagaata tgaccgggac ctggggcacc tcatccccca cgtacacaag ggccaggtgc
     4201 atagggtgcc agaggtgctg gttcatctgc cccgaagggt gcatccggcg cctggaggac
     4261 gacagcctgg agttcgacct ccgctactgc aagggctgcg gcctatgcgc caaggtctgc
     4321 cccaaggagg ccatagccat gcacaggaag ggggcgggcg cgtgagggcg gtgagggcgc
     4381 tcaccggcaa cgccgcggcg gcatatggcg tgaagctttg ccggcccgag gtgatcccga
     4441 actatcccat caccccgcag accatcatca tcgagcagct cgccgatttc ataaacgacg
     4501 gggagctgga cgcggacttc atccctgcgg acagcgagca ctcggccatg agcatcgcca
     4561 tcggcgccgc ggccacgggc gtgcggacgt tcaccgccac cgcctcccac ggcctggcgc
     4621 tgatgcacga gatgctgtac gcccccccgc agctccgcct ccccatcctg ttggtggacg
     4681 tcaaccgttc cctgggggca gggagcggca tctgggtgga gtacaacgac tcgatgcccg
     4741 agcgtgactc gggctggatg caagcgtacg ccgaggatgc ccaagaggtg ctggacatgg
     4801 tcatccaggc ctaccgcatc gccgaggacc cccgcgtggt cctcccgttc atgctctgcc
     4861 tggacggttt catcctctcc cacaccgtgg agcgggtgga ggtgccgggg caggaggagg
     4921 tggacgcttt ccttccaagg ttcagccccc tcaaccgcct ggacccagag gccccgatgt
     4981 gcatgaacat ggccgtctcg cccacgcacg ccatggagat gcgctaccag ctggaccggc
     5041 agatggaagg ggcgaggcag gtgatcgccg aggtggacga agcgttcggc aaggccttcg
     5101 ggcgctccta cggcggcctt ttggacacct acctgatgga ggacgccgag gtcgcgctga
     5161 taaccctggg caccgccacc tccaccgccc gggtcgcggt ggacgagctg agggccgaag
     5221 gaaggaaggt cggcctgatc aagctgcgct tcatgcgccc cttcccgcat gaggagctca
     5281 gggaagcggt caagggcctc agggcgctcg gcgtgttcga ccgctcggtc tccttcaacc
     5341 gcttcgggcc tgtgttcacc gaggtgcgca gctccctcta cggaagcggc gttccgatca
     5401 cggaccacat cgcagggatc ggcggaaggg acctgaaggt ctccacgttc agggatatgt
     5461 tcgccctgct ggagagaagc gcctccgggg ggagggtaag ggaatgcaca tggcacgggc
     5521 tcagggggga gatgtgatgg agcagagggt gaacatacgc tcaatgcccc gggaggacca
     5581 tctcctcagc gggcacggcg cctgcgccgg ttgtggtgtg gccatagcgg tcaagaacct
     5641 catgagggtg cttggaagga acacgaccgt gtacgtgccg gcgagctgcc tcctcatctt
     5701 cggcgggggc atgtaccccc actccgcctt cgacgtgcct ttcctctaca gcgccttcga
     5761 gaacacaggg gcggtcatct ccggcatcaa ggccggctac cgccggcagg ggaaggacgt
     5821 gaccgtggtc ggcatcgccg gggacggggg gacgttcgac atcggcctcc aggccctgtc
     5881 gggggccgcg gagcggaacg aggatgtgat ctacgtctgc gtggacaacg aggcctacat
     5941 gaacaccggg acgcagcgct cggggtccac gccgttcggg gcctggacca ccaccacgcc
     6001 cgtagggagc agggtgaagg gcaagcgcag cttcaagaag gacctcatgg ccgtggtggc
     6061 ctcgcaggac atagcgtacg cggcgacgct gtccatcgcg cacatgaacg acttcgtgca
     6121 gaaggtggag agggccaagg ccatgaaggg cttcaggttc ctgcacatca tggccccctg
     6181 cccgtccggc tggaaggcgg acccatcgct gacctacgac ctggcaaggc tggccgtgga
     6241 gacgggcatg tggtccctgt acgaggtgga ggagggggag gcccatgtca cgtacaggcc
     6301 gaaggagatg ctcccggtcg gggagtatct gaaattgcaa ggccgattca agcacatgga
     6361 cagggaggat gtggaggccc ttcaggcgtg gctgtgccgg aaatggcgca tgcgctatgc
     6421 cccctcccaa cccccgttgg atcgcacgga cgacctggcc tgcgacggcg atgggtgtga
     6481 aggggcgtag ggtctgctct gatgagcggg cctcaggccc cggtcgcgct tccccgcctc
     6541 ctctcgacca gcgtcccgat cgccagggcg gccaccgaca gcggaagcgc gatgaacagg
     6601 gcgtccacca tgtcccaggg gtaggctagc agggatgccc ttccggtcag cagcccggct
     6661 atccccaata cctcggcctc ggccctgtgc acgaagacgg tccaaagccc ccacgtcacg
     6721 ctccccatga ccaggctggc cttggcccca agcgcggagg ggcgctcgga gaacaccgcc
     6781 atcgcgtacg cggggaggaa cgccgccgcg cacaggccca tgaacatggc ggtcgcccgg
     6841 gcgatgatgt cgaagggcat gatgagggcc aggcctaggc tcacggcgat catgacaaag
     6901 gtggcgagct tgttgaccct cagcttggag gcgtcctcgt cgccgccgtg cgccctctcc
     6961 aggaggagct tcgagcggcg gaggtggacc caaatgtcat gcccggccgc cgtgcccatc
     7021 gaatggaaga gcgaactcat ggtggacatg gccgccgcca gcagcaccag cgcgaacatc
     7081 actacgagcc acccgggcat ggccatgtcg atgaagagcg gaacgatgtt gtcgatgttg
     7141 ccggtcatct ccgtggagat cttcccgaag tgctcgtaga agtacacgtt cgtgagcgcc
     7201 cccatcgtgt acgccgcccc cgtggttatc aggatgaaga ccgcccccac cgggacggcc
     7261 cgattgaggg accgcccgtc cccggcggtc atgaaccgca cggccagctg cggctgcgcc
     7321 aggaccccta tccccactcc catgatgatg gtggtgacca aggtatacca ggtcgaggag
     7381 ccgacctccg ggaaggcggt ccatccgacc atcccggcgc tggccaggtc ggccgggacc
     7441 aggggggcca tgtccgtaag ggcctggaac ccgtccagtc cgcccacagc gccgagggtg
     7501 aggaccatga tggcgctcat gccgacaagc atgagggccc cctgcaaagc gtcggtgtac
     7561 atgacagcca tgagcccccc gaagaccacg taggcggcgg tgacggcggt cagcccgatc
     7621 agggcccata ggtaaggaac gtccaggacc atttcgatga agcgcccccc tccaatcagg
     7681 acagccgagg agtacaatgg catgccgatc aggatgacaa ggccggtcgc gtactgcatg
     7741 aaattggacc ggtacacctt gcccagcagg tccgggaagg tcagcgcccc cagtcgcttc
     7801 cctatccgcc tggtcggctt cccgaagacg atgaaggcga tgagcacgcc cacaaggatg
     7861 ttcagcagga cgagccatat gatgcccagc cccagcttgg ccgcccagcc tccgaagccg
     7921 acgatggcgg aggtgctgat gaaggtcgcg ccgtacgaga gcccgatgat caccgggtgc
     7981 acgttcccgc cggcaagcat gtaatcggcg ttgcacttgg tcctcctgta tcccagccag
     8041 gccaggtaga ggagcaccac ggcgtaaccg gcggtggccg cccagaaaag ggtttggtcg
     8101 accacctcag tccacctctt cgttccattt caagatcccg tatattatgc cgaaggctgt
     8161 gctgccgaag cagagcacat acccaagaac tatccaaggg tcctcgatcc cgaacatatg
     8221 aaggtctcct gtcatctggc cagcgattct tagggggatt tgctggacgc tatttaaatg
     8281 ttatacgcta gcatggtgca ggccgccatt accatcccgg actcagtaga cgcgcttgtc
     8341 gatgaccctc ttggccttcc cctcgaagcg ggggagcgac cccggctcta ctatctccac
     8401 gctcaccccg acgttgaggg agtcgcgcag cctgtgggcg aggcggtcct tgatggcgtc
     8461 gagctcgaag agccggtcgc cgaaggcctc cggcctgagc tccaccttga ccagcatggt
     8521 gtccagggcc cccctgcgct ccaccacgat ctggaactcg ttgccgacct cggggtcggt
     8581 catcagggcg tgctccacct gggacgggaa cacgttgatg ccgcggacga cgatcatgtc
     8641 gtccaccctg ccctggatcc tcccgatgcg cggatgcgtg cgcccgcagg cgcagacctc
     8701 ctcggtgagc gtggtgacgt cccccgtccg gtagcgcacg atagggaagg cctccttctc
     8761 cagcatggtc actaccagct cccccttctc ccccggcggg acgggctccc ccgtcgcggg
     8821 gtccaggacc tcgacgagcg tcatgtcccc ccacacatgc atccccttct gctcggagca
     8881 ctcgcaccag agcgggccgg acagctcgct cgtcccatag atgtccacgg ccttgatgcc
     8941 gaggcgctcg tggatccggt cccgcatccg gtcgctccag ggttcagccc ccaggatccc
     9001 cagccgcagc tgcgtgtccc tctcgatgga gacgcccatc ctctccgcca cctccccgat
     9061 gtgcaggagg tacgaggggg tggaggcgat gacggtgacg ttcaggtcgc gtatgaggtc
     9121 gatctgcctc tccgtgttcc cggtggaggc gggcacgacg gcggccccga ccttctcggc
     9181 gccgtagtgc aggccaagcc ccccggagaa cagcccatag gtgtagcaga cctgcagcac
     9241 gtcatcgggg cccaccccgg cggacaccag ccctcgggcc accgattccg accaggaatc
     9301 gatgtcgttc cgggtgtagc ccacgatggt cggcttcccg gtcgtgccgg aggaggcgtg
     9361 gtaccgcacc acctccctgg ggctcgcgag gaaaagcttg tccggatagc tgtcccgcag
     9421 gtccctcttg gtcatgaagg gtaatctccg gacgtcgtcc agcccctgaa cgtcctcggg
     9481 cgtgaccccc gcctccctca tcctctgccg atat
//
LOCUS       DAOSYN010000031         9134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_6467_length_9134_cov_3.453023, whole genome shotgun sequence.
ACCESSION   DAOSYN010000031 DAOSYN010000000
VERSION     DAOSYN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_9134_cov_3.453023"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2810
                     /locus_tag="PLJ11_02490"
     CDS             <1..2810
                     /locus_tag="PLJ11_02490"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOO03568.1"
                     /translation="ECVAFLNTAYGDICVQKYEDANGNGEVDEGDTIVPGVLITLYDA
                     QMSAIANGTTGDDGRICFCGLELGTYYVMETLSDEWYAQGPTLKQVVIGESGESVSES
                     FLNIRYGKICVFKFEDLNGDGVWQEGEPGLEGIVIELVQDCVVIRSGETNESGWICFD
                     LLRFGTYWARENLAASDMDCCWYPSTPDRVQVTLSGEDRCELVVFGNVMYGKICGWKY
                     FDANENGQFDPCTESGIAGWEIELWLDGKLFAMTHTDSHGHFCFAGLEFGDYTVTEVM
                     QDGWYATGPTSWNVTIDVSGDREDVFFLNAMCGKICVLKYEDLDGDGILDEDEPAVPG
                     ITVLLYRDDVKVGEGVTDGNGSYCFEDLMVGTYAVVEEIPCNWYSTTGVVQQVVMTGQ
                     DATVTFLNTKYGCIGGFKYNDLDGDGVWDPTTEAGIPEWEIWLYQNGKPVSWTVTDLS
                     GHYAFCGLRLGEYEVREGRQEGWMATSATELPVTIETSGQLVCGVNFFNMELDPCIGI
                     EKGVCSDTIEYESHTQGYWKNHECSWVGMGPNGAFPWVCSCAYPDGLTNLQVFNTPPA
                     GDATLILAHQYLAAKLNDLKYGAPAMYVQLISDAEGFLAIHPIGCSLSDDEREQATEL
                     ADLLATYNEGEDSGMTVLTYTVVVTNCGNVELYNVKVYDSLLDQWFFLCDFGDGDGIL
                     DVGEWWTFSYTYIVPADQCGEEETCDDDDGCHDGGCGGWTWNWDHGCGGGCHDGDEND
                     DGWSWSWGCGNHEGRTDGGCGGWNWRHGYGSGCGGWHWNWNWQQHCGCCCDDEVPTET
                     CIVTNIATAYGQWGPEAEGWWVSDQAQADFELQQEEPIDDDDGCHDWGCGGWQWQWGW
                     NWGCCWDHDWGCGNDRDCDQGWNWGGCSNYRYLNFGCFRWSGTFNDGMIGGCHVWNDY
                     GPFEDERRGKEETEVSETEEK"
     gene            complement(2891..3011)
                     /gene="rrf"
                     /locus_tag="PLJ11_02495"
     rRNA            complement(2891..3011)
                     /gene="rrf"
                     /locus_tag="PLJ11_02495"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(3064..3519)
                     /locus_tag="PLJ11_02500"
     CDS             complement(3064..3519)
                     /locus_tag="PLJ11_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03569.1"
                     /translation="MGEEAFGAYLVLRWGDPADVTEYLCHLVRDGFEPVLVSPADMVL
                     PSDMEIEALPPEQGERNLGKGSGPRICRALREHLRTGERRAVVIYGLQLLEEGSFREL
                     TDMIDMLYDEACVNHGLVLLFADPAGFTPQELAFLRRETSAIERPEQLP"
     gene            complement(3566..4285)
                     /locus_tag="PLJ11_02505"
     CDS             complement(3566..4285)
                     /locus_tag="PLJ11_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOO03570.1"
                     /translation="MIALEQAAAVLVLCAALSALSWKLGLLTADGSLASFAVGMVLGV
                     FGGVGWLFLLILFAFMGFLVTKYKMELKVRKGVQEGRKGERGYRNVLANGAVPMAIAL
                     LTYAAGWEGHASAVVYISAVCVAAADTTASELGVLSDRTYLITTFKRVRPGVDGGVSL
                     YGTAWAVAASAFAAVVGWFVIIGTEPTMLMLVPVGMGVAGCFIDSVIGATLETKGWVT
                     KLGNNIVSMALGALLSLPFLP"
     gene            complement(4312..8994)
                     /locus_tag="PLJ11_02510"
     CDS             complement(4312..8994)
                     /locus_tag="PLJ11_02510"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO03571.1"
                     /translation="MRRVAAAVLIVLFTFSTAVPGICSQIEHPLDLQENQSLADWELG
                     SGNFNGLSQTFVVAGEPLKHVVADLNNDSRPDIAVIYNASFRIDIFYNDANGRFNYSD
                     RTTIDMPGPVTDIAAGDVNKDLKQDLVVSCNVTGNDVFVLSQANNFDKNAPLYAIPTT
                     SKPRALILQDLNGDSWLDIATLYYGDPPDYLPGFEVRLKSEDYDSAGSFPLYMDPLNM
                     QQPSLFTSGDFNQDGRSDLVIGDRAIGKVVGFINGAPSGKVWTNTTNLIPANAPSALC
                     MAQLIAGGGMDLMVAQEDSSIINIWRYSEGTFNLRTNVMDQESITSLATTDVNGDSRL
                     DLVSVSASKHNLTVFRASTSLLYGHSNSLAFPVPYHPVHVIAYDMDSAGSEDLVVSSR
                     ASTGYGAISIYYRNGQEISNADLNRIVKDISPTLMTLGNFNGIGNNEIAVYDPTGPTI
                     SFIDEASNPGRPSAPSGILAIKSGDLDGDGYDDIAMMTANNVTVWFGGPEIFSGASRK
                     VTVESTLPGLSSLFLGDLNNDSRLDVAVGASGGVESFWNSGTGTLYSEAQRFTLSLPG
                     ASVTSLATVTIRGAVHNDSRADLVVLNSTASRLEIYYQQSAGSVFTAASRSYLNVISG
                     LYHLRAGDLNSDGLGDLAVLSPGKVNIYLQNPSYSQGFSGSQPIVAIDVPDGPRALEL
                     GDLDDDGALDIAIVTENSTLIAYGHDGPSFSLLTRQTAGAAPVLLSVGDMNGDGKDDL
                     VTYSVASRAVSFYHQNNFPPIAMVAVEGTGHLEGGPVWFNAYGSTDSYSDRDHLSYHW
                     DFADGGVANGERVLYVFQADGLYNVTLSVSDPWGGWDDAVVQVSIGDRAPTANFTFQS
                     VPAPVEGSPVTFTDLSTTPVDPIKRWEWDFGDGTVLNRTDNQPVQHIFGENGAFTVTL
                     TVFDKDGSSNSVEKQLTVLDSAPEAAFSVSNYRPIEGQEVTFRDWSNRTADAIKSWSW
                     DFGDGTWANVSGGDLQHHAYLRNGTYSVTLVVVDIDGSEDSITKEVVVLNSVPVADFE
                     PSLSTPNEGQQVSFVDASTSFNPMVRWSWDMGDGTWCNGTSAETVLHMYRDNGTYIAT
                     LTVEDSDGDGHSISKPIIVRDTSPTIVRLYTMDGGSSYREWDNVSFGVVATPGWEDIS
                     VYQWDFQTTVFQADEGTALNSTYHRFLSPGPYKVTVRVWDTDSYAEAYIMLTITDPAP
                     VPDFTATVTGHDRNVSFSAALTVDTENDVPILKYRWFFGDGNRTDWSSYFEVDHTYQQ
                     DGVYSVRLEVRDDHNSAVMKTKNVTIDLLPPEISIDAPVLRAVVGEPTLIKVRVTDLV
                     GVRSVILEYTFENATRSVAMTPEGGGIYFAQVPAQNRTGELTYRVTAVDKAGHASSTE
                     WFTVALEYEDPTLFIVTTALLLIVLLIVIAYLVLTRPIVDEVFVMYQDGTLLAHQTRR
                     LKPGMDDEILGGMLIALQSFVRDSFKDEGATVLKRMDFGDRKLLVERKDGFFMAVVLS
                     GKRAGNSAQRMRRVLDRIEERYADVLRDWDGDLEKVRGIRDETKLMFQRTNPLERLRR
                     REGDEGST"
BASE COUNT         1817 a         2763 c         2849 g         1705 t
ORIGIN      
        1 gcgagtgcgt ggccttcctg aacaccgcgt acggtgacat ctgcgtgcag aagtacgagg
       61 atgccaacgg caatggtgag gtggacgagg gcgacaccat cgttccaggc gtgctcatca
      121 ccctgtacga cgcacagatg agcgcgatcg ccaacggcac caccggcgac gacggccgga
      181 tatgcttctg cggactggag ctaggcacct actacgtgat ggagaccctg agcgacgaat
      241 ggtacgccca gggccccacg ctcaagcagg tggtcatcgg cgagtccggc gagagcgtga
      301 gcgaatcgtt cctgaacata cgctatggca agatctgcgt cttcaagttc gaggacctga
      361 acggcgacgg cgtgtggcag gagggcgaac ccgggctgga aggcatcgtg atcgagctgg
      421 tccaggattg cgtggtcata cgcagcggcg agacgaacga gagtggctgg atctgcttcg
      481 acctcctgcg cttcggcacc tattgggcga gggagaacct ggccgccagc gacatggact
      541 gctgctggta cccatccacc ccggaccggg tccaggtgac gctgtccggc gaggaccggt
      601 gcgagctggt cgtcttcggc aacgtcatgt acggcaagat atgtggctgg aaatacttcg
      661 acgccaacga gaacggtcag ttcgacccgt gcaccgagtc gggaatagcc ggctgggaga
      721 tcgagctgtg gctggacggc aagctgtttg ccatgaccca caccgacagc cacgggcact
      781 tctgcttcgc cggcctggag ttcggtgact acaccgtgac ggaggtcatg caggacggct
      841 ggtacgccac cgggccgacc tcatggaacg tcaccatcga cgtgtccggc gaccgcgagg
      901 acgtgttctt cctgaacgcc atgtgcggga agatatgcgt cctcaagtac gaggacctgg
      961 acggcgacgg tatcctggac gaggacgagc cggcggtgcc aggcataacc gtcctcctgt
     1021 accgggatga cgtgaaggtc ggagaaggag ttaccgacgg gaacgggagc tactgcttcg
     1081 aggacctcat ggtcggaacg tacgcagtgg tggaggagat accctgcaac tggtactcca
     1141 ccaccggggt cgtccagcag gtggtcatga ccgggcagga tgccacggtg accttcctga
     1201 acaccaagta cggctgcatc ggcggcttca agtacaatga cctggacggg gacggtgtct
     1261 gggaccccac caccgaggcg ggcatacccg aatgggagat atggctgtat cagaacggca
     1321 agccggtctc ttggaccgtc accgacctca gcgggcacta cgccttctgc ggcctgcgcc
     1381 tgggcgagta cgaggtgagg gagggacggc aggagggatg gatggccacc agtgccacgg
     1441 agctaccggt gaccatcgag acctccggcc agctggtctg cggcgtgaac ttcttcaaca
     1501 tggagctgga cccatgcatc gggatcgaga agggggtgtg cagcgacacc atagagtacg
     1561 agtctcatac ccagggctac tggaagaacc atgagtgctc ctgggtgggc atggggccga
     1621 acggggcgtt cccctgggtc tgctcctgcg cctaccccga cgggctgacc aacctgcagg
     1681 tgttcaacac cccgccggcc ggtgacgcca cgctgatact cgcccaccag tacctggcgg
     1741 ccaagctgaa cgatctcaaa tacggcgccc cggccatgta cgtccagctg atatccgacg
     1801 ccgagggatt cctggccatc catcccatag gctgctccct gagcgatgac gagcgggagc
     1861 aggccaccga gctggccgat ctgctcgcca cctacaacga gggcgaggac agcggcatga
     1921 ccgtgctcac ctataccgtg gtggtgacca actgcggcaa tgtggagctg tacaacgtca
     1981 aggtgtatga ctctctgctc gaccagtggt tcttcctctg cgacttcggc gatggcgacg
     2041 gcattctcga cgtcggcgag tggtggacct tctcctacac ctacatagtc ccggcagacc
     2101 agtgcggcga ggaggagacc tgcgacgacg atgacggatg ccacgacgga ggctgtggcg
     2161 gctggacctg gaactgggac cacggctgcg gcggaggctg ccatgacggc gacgagaacg
     2221 acgatggctg gagctggtcc tggggttgcg gcaaccacga gggacgcact gacggaggct
     2281 gcggcggatg gaactggcgg cacggctacg gaagcggctg cggcggctgg cattggaact
     2341 ggaactggca gcagcactgc ggatgctgct gcgacgacga ggttccgacc gagacctgca
     2401 tcgtgacgaa catcgccacc gcctacggcc aatggggccc agaggcggag ggatggtggg
     2461 tgagcgatca ggcccaggcg gacttcgagc tgcagcagga agagccgatc gacgatgatg
     2521 acggatgtca cgactggggc tgtggcggct ggcagtggca atggggctgg aactggggtt
     2581 gctgctggga ccacgactgg ggctgcggca acgaccggga ttgcgaccag ggttggaact
     2641 ggggcggttg ctctaactac cggtacctca acttcggctg cttcagatgg tcgggcacgt
     2701 tcaacgacgg aatgattggt ggctgccacg tatggaacga ctacggtccc ttcgaggatg
     2761 aacgccgggg caaggaggaa acggaggtct ccgagaccga agagaagtga gcgttgaaac
     2821 caggggggcg accccctgcc catttttttt tcatataacg ccctgttctt agaaaaatga
     2881 aaggtaagga ggctgacagc gttccgggct ttccgtgggc tcgcgcacca cagtaccgca
     2941 gggaacgcgg gcgggcttag cttctgggat cggacgagac caggcgtatc cccgccgcta
     3001 tggccgtcag accatccttg agcctcctga aaaaggccaa tgtatataac agtttcccaa
     3061 ggcctacggg agctgttccg ggcgctcgat cgccgaggtc tcccgcctaa ggaacgccag
     3121 ctcctgaggg gtgaagccgg cgggatcggc gaagagcagt accaggccat gattgacgca
     3181 cgcctcgtcg tacagcatgt ctatcatatc ggtgagctcc cggaacgacc cctcctccaa
     3241 tagttggagc ccgtagatga ccaccgccct cctttccccg gtgcgcaggt gctccctcag
     3301 ggcccggcag atgcgcggcc cgctcccctt ccccaggttc cgctcgccct gctccggcgg
     3361 aagggcctcg atctccatgt ccgaagggag caccatatcg gcgggggaga ccagcacggg
     3421 ctcgaagccg tccctcacca gatggcaaag gtattcggtg acgtcggccg ggtcccccca
     3481 tcgcagcaca aggtacgcgc cgaaggcctc ctcgcccaac cgctcaccct atgcaatagt
     3541 aggcctggct ctattaatgg tttgttcagg gcaggaaggg gagggagagc agggccccaa
     3601 gggccatgga cacgatgttg ttccccagct tcgtgaccca gcctttcgtc tccaacgtgg
     3661 ccccgatgac gctgtcgatg aagcatcccg ccacgcccat gcccacggga acgagcatca
     3721 gcatcgtcgg ttccgtgccg atgatgacga accagcccac caccgcggcg aacgccgagg
     3781 cggcgacggc ccacgccgtt ccgtagaggg aaacgcctcc gtccacgcca ggccggacgc
     3841 gcttgaaggt ggtgatcagg taggtccggt ccgagaggac ccccagctcg ctggcggtcg
     3901 tgtccgccgc cgccacgcac accgcgctga tgtagacgac ggccgaagcg tgcccctccc
     3961 atcccgccgc ataggtgagc agggcgatgg ccatcgggac cgcgccgttg gccaggacgt
     4021 tgcggtagcc cctctccccc ttcctcccct cctgcacgcc cttccgcacc ttcagctcca
     4081 tcttgtattt ggtgacgagg aaccccatga aggcgaaaag gatgagcagg aaaagccatc
     4141 ccaccccccc gaagaccccg aggaccatgc ccacggcgaa ggaggccagg ctgccgtcgg
     4201 cggtgaggag cccgagcttc caggaaagcg cggagagcgc cgcgcagagc acgagcacgg
     4261 ccgccgcctg ctccaaagcg atcattgcgc ccctttaaag gtccagcata tttaagtcga
     4321 cccctcgtcc ccttccctgc gcctcaggcg ctccaacgga ttggtgcgtt ggaacatgag
     4381 cttagtctcg tccctgatcc ccctcacctt ctccaggtcc ccgtcccagt cccgcaggac
     4441 atcggcatac ctttcctcga tgcggtccag gaccctccgc atccgctgcg ccgagttccc
     4501 cgccctcttc ccgctcagga ccacggccat gaagaagccg tccttgcgct ccacgagcaa
     4561 tttcctgtcc ccgaagtcca tgcgcttgag caccgtggcc ccctcgtcct tgaacgagtc
     4621 gcgcacgaag ctctgcaggg cgatgagcat gccgcccaat atctcgtcgt ccatgcccgg
     4681 cttcagcctc ctcgtctggt gcgcgaggag cgtgccgtcc tggtacatga cgaagacctc
     4741 gtcgacgatg gggcgggtca ggacgaggta ggcgatgacg atcagcagga cgatcagcag
     4801 aagggccgtg gtgacgatga acagcgtggg gtcctcgtac tccagggcga ctgtgaacca
     4861 ttccgtggag gatgcgtgcc ccgccttgtc cacggcggtc accctgtagg tgagctcccc
     4921 ggtgcggttc tgcgccggga cctgcgcgaa gtagatgccc ccgccctccg gggtcatggc
     4981 aacgctcctc gtggcattct cgaacgtgta ctccaggatg accgagcgaa cgcctaccag
     5041 gtcggtgacg cgcaccttga tcagggtagg ctcgccgacc acggccctca ggaccggggc
     5101 gtcgatggag atctccggcg gcagcaggtc gatcgtaacg ttcttcgtct tcatgacggc
     5161 gctgttatgg tcgtccctga cctccaggcg gacggagtac acgccgtcct gctgataggt
     5221 atgatcgacc tcaaaataag atgaccagtc ggtccggttc ccgtccccga agaaccagcg
     5281 gtacttgagg ataggcacgt cgttctcggt gtccacggtc agggcggccg agaagctcac
     5341 attcctgtca tggccagtga ccgttgccgt aaagtctggg acgggggctg ggtctgtgat
     5401 cgtgagcatg atgtacgcct cggcatagct atcggtgtcc cagacccgca cggtcacctt
     5461 gtaggggccc ggggagagga agcggtgata ggtcgagttc agtgcggtcc cctcgtccgc
     5521 ctggaacacg gtggtctgaa agtcccattg gtagacgctg atatcctccc agcccggcgt
     5581 ggcgacgacc ccgaaggaca cgttgtccca ctccctgtag gagctgccgc catccatggt
     5641 gtagaggcgc acgatcgtcg ggctggtatc gcggacgatg atgggcttgg agatcgaatg
     5701 cccgtcccca tcgctgtctt ccacggtaag ggtggcgatg taggtgccat tgtcccggta
     5761 catatggagg accgtctccg cgctcgttcc gttgcaccat gttccatccc ccatgtccca
     5821 actccaccgg accattggat tgaaggacgt gctcgcatcg acaaaggaga cctgctggcc
     5881 ctcattaggg gtcgagaggg agggttcgaa gtcggcaacg ggcaccgagt tgaggaccac
     5941 gacctccttg gtgatgctgt cctcacttcc atcgatgtcc acgacaacga gggtgaccga
     6001 gtaggtgcca ttgcgcagat aggcatgatg ctggaggtcg ccaccgctta cgttcgccca
     6061 cgtgccatcg ccgaagtccc agctccagct cttgatggcg tcggcggtgc gattgctcca
     6121 gtccctgaag gtcacttcct gcccctcgat cggacggtag ttcgataccg agaaggccgc
     6181 ttcgggtgcg gagtccagga ccgtcaactg cttttccacc gagttactgc tgccgtcctt
     6241 gtcgaacacg gtcagggtta cagtgaaggc cccgttctcc ccgaagatgt gctgcacggg
     6301 ctgattgtcg gtacggttca acaccgttcc gtccccgaag tcccattccc agcgctttat
     6361 cgggtccacc ggagtggtgc tcagatctgt gaacgtcacc ggggacccct cgaccggggc
     6421 cgggacggac tggaaggtga aattggccgt cggggctcgg tcccctatag aaacctgcac
     6481 caccgcatcg tcccaacctc cccagggatc gctgacgcta agggtaacgt tgtacaggcc
     6541 atcagcctgg aagacataaa gaacacgctc gccgttcgcc accccgccgt cggcgaaatc
     6601 ccaatggtaa ctcagatggt cccggtccga gtaggaatcg gtgcttccat aggcgttgaa
     6661 ccagaccggg ccgccttcca ggtgacccgt cccctccacc gcgaccatgg cgatgggcgg
     6721 gaagttgttc tgatggtaga acgataccgc ccgggaggca acggaatacg ttaccaggtc
     6781 atccttcccg tccccgttca tatcccctac cgaaagcagc acgggggcag ccccggcggt
     6841 ctgcctggtc agcagggaga aggagggacc gtcatggcca taggctatca gggtggagtt
     6901 ctccgtgacg atggcgatgt ccaacgcccc gtcgtcgtcc aggtccccca gttcgagtgc
     6961 tcgcggtcca tctggaacgt ctatcgccac tatgggctgg ctgcccgaga aaccctggga
     7021 ataggaaggg ttctgcaggt aaatgttgac cttgcctggg gacagcacgg ccaggtcccc
     7081 aagcccatcg gagttcaggt ccccagcgcg caaatggtac aaccccgaaa taacattcag
     7141 gtacgacctc gaagcggcgg tgaacacgct gcccgcgctc tgctggtaat aaatctccaa
     7201 ccttgaggcg gtggagttca agacgaccag gtccgcgcgg gaatcgttat gcacggcgcc
     7261 cctgatcgtc accgtggcca aggaggtcac cgaggccccc ggaagggata aggtgaagcg
     7321 ctgagcctcg gaatacaagg tcccagtccc ggaattccag aacgattcca cgccgcccga
     7381 ggcgcccact gccacgtcca gtcggctatc gttgttcagg tcgcccaaga acagggagga
     7441 cagcccagga agggtgcttt ccaccgtgac cttcctagat gcgccactga agatctcggg
     7501 gccaccgaac cagacggtga cgttattggc ggtcatcatc gctatgtcgt cgtacccgtc
     7561 cccgtccagg tccccgctct ttatagctaa tattcccgag ggggcgcttg ggcgccctgg
     7621 gttggaggcc tcatcgataa aggaaatggt aggcccagtg ggatcgtaga cagcgatctc
     7681 attgttccca atgccattga agttccccag cgtcatcaag gtgggggaga tatcctttac
     7741 gatgcggttg aggtcggcgt tcgagatctc ctggccatta cggtaataga tgctgattgc
     7801 cccataaccg gtcgaggctc gagaactgac aaccaggtcc tccgaccccg cggagtccat
     7861 gtcgtaggcg atcacatgga ccgggtggta tgggactggg aaggccaatg aattggaatg
     7921 gccgtacaga agggatgtac tggcccggaa aacggtcagg ttatgcttgg aggcggatac
     7981 ggagaccagg tccaagcggg aatccccgtt cacgtccgtg gtggccagcg aggttatcga
     8041 ctcctggtcc atcacattag tacgtaagtt gaatgttccc tcggaatagc gccaaatgtt
     8101 gataatcgag gaatcctcct gagccaccat gaggtccatg cccccgcctg caataagctg
     8161 agccatgcac agcgcggatg gagcgttagc gggaatcagg ttggtcgtgt tggtccatac
     8221 ctttccgctg ggcgcaccgt tgatgaaacc caccaccttt ccgatcgcgc ggtcgccgat
     8281 taccaggtcg cttcggccat cttggttaaa atccccggaa gtgaataggg aaggttgctg
     8341 catgttcaat gggtccatgt acaatgggaa ggagcccgct gaatcatagt cctcggattt
     8401 taaacggact tcgaaccctg gcagataatc gggagggtcc ccataataga gggtagctat
     8461 gtccagccag gagtctccgt tgagatcttg gagtatcaac gcccttggct tagaggtggt
     8521 gggtatagca tataacgggg cgttcttatc gaagttgtta gcctgggaga gaacgaacac
     8581 atcatttcct gtcacattgc aggagacgac caggtcctgt ttcaggtcct tgttcacatc
     8641 tccggccgct atgtctgtta ccggacctgg catgtctatc gtggtccgat cactgtagtt
     8701 gaatcgccca ttggcgtcgt tgtagaatat atcgatgcgg aaggaggcgt tgtagattac
     8761 ggcaatatcc ggccggctgt cgttattaag gtcggccacc acatgcttca acggctcccc
     8821 tgccaccacg aacgtctggc ttaggccatt gaagttaccc gaacctaatt cccagtccgc
     8881 cagagattgg ttctcctgga ggtccaatgg gtgctcgatc tgcgagcata tgcctgggac
     8941 ggcggtgcta aatgtgaata aaactatcag cacggctgcc gctacgcgcc tcatgcccac
     9001 ccctcaggaa ccgatattca tctatgctga taaataattt ccctatgact ggatgggcgt
     9061 ttcggtctta tattgccggg aagggcgctc attcccggac catgagcgcg aacagcctct
     9121 gcgcaagctc cttg
//
LOCUS       DAOSYN010000032         9017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_6572_length_9017_cov_4.547646, whole genome shotgun sequence.
ACCESSION   DAOSYN010000032 DAOSYN010000000
VERSION     DAOSYN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6572_length_9017_cov_4.547646"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PLJ11_02515"
     CDS             <1..1206
                     /locus_tag="PLJ11_02515"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOO03572.1"
                     /translation="TSEAHMKHKLKMTREQVKTRAVDAVEYARSRGVTVEFSCEDGTR
                     TDLDFLKEMHIAVQEAGVHKINLPDTVGTMSPAAMEYLVAEVMKVTKVPLSIHCHDDF
                     GLAVANSLAAVRKGARQVHVCVNGLGERAGNAALEEVVMGLLALLNVRTNIDTRKLGM
                     TCRSVARMTGIPIPDTKAIVGNNAFAHESGIHVHGVLSDPSTYEALSPELVGMQRNIV
                     MGKHSGAHSVKEKLAEYGISLPEGMLSTVVEKVKSLAESGKEVDDAELVALALHIQGQ
                     AEKERQHVKLKEFAVFTGMNITPTSTVAIEVDGKQVRSSNVGIGPVDAALNAIRTAVS
                     ENISLVEYKLNAITGGSDALCEVTVKMRMNDDQKVMSVGKSIGSDIVLTSVDAAMGAI
                     DRLLARHRE"
     gene            1217..2485
                     /locus_tag="PLJ11_02520"
     CDS             1217..2485
                     /locus_tag="PLJ11_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOO03573.1"
                     /translation="MRTDPKTISEKILSLRSGQDASAGDIVEADVDYVMVNDVTGPLA
                     FQEFEALECEPVRDRIVLIPDHFVPNKDVASAKQAKEMREFAKRWGIRNYFEVGRGGV
                     CHQVMLDNGFAYPGALVVGADSHTCTYGGVNAFATGVGSTEAAAVFATGHLWFKVPSS
                     IRVLLKGRPSRYIGGKDLALKLISDIGVDGATYKALEFGGTGVSALTVSDRLSVANMA
                     IEAGGKAGIFPCDAATVAYMKTVRKGGFKAALPDEGAVYERTLEYDLGELESMVALPH
                     LPSNGRAARDVNVPIDQAYLGSCTNGRIEDLRTAAEVMRGRKVHPDVRMVVVPASVRV
                     FEQALAEGLLTELSKAGAFISGPTCGACLGGHMGILAPGERCVSSTNRNFIGRMGHRD
                     SEVYLAGPAVVAASAVTGRITDPRDLEAVR"
     gene            2482..2976
                     /locus_tag="PLJ11_02525"
     CDS             2482..2976
                     /locus_tag="PLJ11_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOO03574.1"
                     /translation="MREVKGHVWRFGDHVDTDQIIPAERLTSENNAILGTFAFEKVRP
                     GMAEQVKQGDVIVAGRNFGCGSSREHAPRALLQMGIACVVAESFARIFYRNCINTGLL
                     PVECRIEADDGDLLSVDLAKGTIVNHTKGKEWGFTPFPSFIQELMDKGGLMAKIKEEK
                     RCTR"
     gene            2964..4079
                     /locus_tag="PLJ11_02530"
     CDS             2964..4079
                     /locus_tag="PLJ11_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOO03575.1"
                     /translation="MHKIAVLPGDGIGPEVVAEGLKVLNALSENYSVKFDFQEFDISS
                     ERYLRTGKLVTAEDMDQLRKFDSIFLGAIGDDRVKPGILEKGILLALRFGFDQYINHR
                     PAPLWRPFGRLKRDVDFNIDVFRENTEDYYIGAGGRVNNGRGSLDLHVKRHLYDMKVR
                     LEANADAADDYAFEIGMMSRKNIERFADHVIAFSNIIGENYITVVDKANVCSNIYSLW
                     REVWDEKCKKAGKGHGYMYVDAMTMALVKNPDKFRVIAVPNMFGDIITDLLAEVTGGL
                     GLAPGGNINPEGISMFEPVHGSAPKYKGMNRINPVATILAAKMMLDSLGHRDLGQVVQ
                     QAVRTAFDQGVYTQDLGGKATTTEMGDAVVEAIRKAK"
     gene            complement(4228..5298)
                     /locus_tag="PLJ11_02535"
     CDS             complement(4228..5298)
                     /locus_tag="PLJ11_02535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO03576.1"
                     /translation="MRIVEANAFHYPFVGGIENRLHHVCRRLGRRHEVFVLTSRLPGT
                     AEREEMDGYTVVRLPSRFIDVYNPPYVHITKVKEAFRGLAPDIVDLHYRWAGSLTKGV
                     LAFPGPKVYTCHNTLGEGVGLTHYASELNDRLFLRHLHAFDRIVCVSDYMRRDVAARG
                     FPDRQLTTIYNGVELPQGPVKDGDFILSLGRLVRLKGLDVLIRAMKHTRCQLKICGEG
                     PERKRLEGLSHRLMLTDRIDFLGWVSEAEKHRLLSECRMFVIPSLHEAYGMVAAEAMA
                     YGKPVIASDTGGLPEVVGDAGVLVPTGDVEGLARAINALDGDQEGRDRLGRAARERMR
                     AFTWDEIADRTEELYLEVAGRR"
     gene            complement(5309..7144)
                     /locus_tag="PLJ11_02540"
     CDS             complement(5309..7144)
                     /locus_tag="PLJ11_02540"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF003068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOO03577.1"
                     /translation="MRTLYIHADFMEYEVKKPTPMAEAVADQERTGRMEEVLVAFLTV
                     ERGDQDKVEAVASQASADIIGTAEKVGAERIMLYPYAHLSPDLADAETGKRVLREIER
                     MIAAKGWQVHRAPFGWYKAFRISCKGHPLSELSREIVGEEAPPAPKGEERFLVLLPDG
                     SEVDPRGFEGGSECFQIMMAKEALKKEWPSTGEVKYTRLCKKFGINWESMSDAGHMCF
                     SPKGALMFDLVADYSARIVNSLSLPVYTVRGTNMFSLDEGPVAEHAKLFGDRLYVIKG
                     EKRDFVMRYAACHQQFAMMRLWNISYKNLPFGAFEVADSYRMEQSGETMLCFRTRRMN
                     MPDLHVVCRDIPEAEEWFSRLDGRIFDEANALGRDYEMLINFSSWDAYQKHKGLIMEI
                     VRKHARPALLHFYPEGINYYWTVNIEYHILDDMKRAREIGTVQIDIGNAQRFGIAYTD
                     ESGKKTFPVILHTAVIGTVERYLYTLFDTAVQMETRGKVGTLPTWVNPEQVRFMNVSE
                     AHLERARELADRVRRANVRVGLDDRSETVGKKVREAKQDWVGYAIVIGDKEMGSDVLT
                     VYDRSQNRNVEMTVDQLIERVRGEIGDMPFRPMYLPSELSRRVDM"
     gene            7292..8179
                     /locus_tag="PLJ11_02545"
     CDS             7292..8179
                     /locus_tag="PLJ11_02545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOO03578.1"
                     /translation="MRSSRIARDIGGATNVLLIVLIAVLMVSASTVAIMLYQGGQSVE
                     AAENVVTVGQTIKVDYIGKLLDERVFDTSLYSVASNDAAYPKTLSFTLKANTSYVPLT
                     FKVGAGTMIPGFETAVVGMRVGETKTVTLPPEEAYGPMDQSKLVTFQLTEAVPLVRIF
                     NASTFKAEYGEDAASGLTVYDPVYGWAATVMEYNAWTDRVTVKNAPTLNALYHIYGTG
                     SVGWDVKVIGMDSNSNRITIQHQLGEGDSDRVKGLDGSSTFFIIDVDEEKGTAVRNYN
                     SELAGKTLVFTITIVAIVE"
     gene            complement(8191..8817)
                     /locus_tag="PLJ11_02550"
     CDS             complement(8191..8817)
                     /locus_tag="PLJ11_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03579.1"
                     /translation="MFSIGEAFYVLFSPLGLAGWMVCTFLLFYIDAIVFPTLPELFVV
                     LFFMSGYGTVPDWALWSMFVVTIAVAEVLGLTTLYMVVKRLRVPPKIATVVDRYRRFL
                     LIPDERMILLNRIAPILPFTGAFVALSGWSYRRSIFYQVLGGTLKYGSILAASNYFLA
                     YMERGLAADVTLAMVIIIIVLSFIASTYRKRKVDRLDVAGTGEDGGPR"
     gene            complement(8833..>9017)
                     /locus_tag="PLJ11_02555"
     CDS             complement(8833..>9017)
                     /locus_tag="PLJ11_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03580.1"
                     /translation="IQLVPGHASVAEGPERYREGALAKAREFSAGQWATRLVDVYRYA
                     IEAKAGRANDKAFLKR"
BASE COUNT         1748 a         2829 c         2873 g         1567 t
ORIGIN      
        1 acctccgagg ctcacatgaa gcataagctc aagatgacga gggagcaggt caagacccgc
       61 gcggtcgatg ccgtggagta cgcccgctcc cgcggggtga cagtggagtt ctcctgcgag
      121 gacggcacgc ggaccgatct ggacttcctg aaagagatgc acattgccgt gcaggaagcg
      181 ggggtgcaca agatcaacct gcccgacacc gtaggcacca tgtccccggc ggccatggag
      241 tacctggtgg ccgaggtcat gaaggtcacg aaggtccccc tatccatcca ttgccatgac
      301 gatttcgggc tggccgtggc gaactcgctg gcggcggtgc gcaaaggggc gcgtcaggtg
      361 cacgtctgcg tcaatggtct tggggaacgg gcggggaatg cggccctcga ggaggtggtc
      421 atgggactgc tggccctcct caacgtacgg accaacatcg acaccaggaa gctggggatg
      481 acctgcaggt cggtggccag aatgactgga atcccgatcc cggacaccaa ggccatcgtg
      541 ggcaacaacg ccttcgccca tgaatcgggc atccacgtcc acggggtact gagcgatccc
      601 tccacctacg aggcgctgtc ccctgagctg gtgggcatgc agcgcaacat cgtcatgggc
      661 aagcattccg gtgcccattc cgtcaaggag aagctggccg agtacgggat ttccctgccc
      721 gagggaatgc tttccacggt cgtggagaag gtgaagagcc tggcggagag cgggaaggag
      781 gtggacgacg ccgagctggt cgcgctcgcc ctgcacatac aggggcaggc ggagaaggag
      841 cggcagcacg taaagctaaa ggagttcgcg gtgttcaccg gtatgaacat cactcccacg
      901 tccacagtgg cgatcgaggt ggacggcaaa caggtgcggt cctccaacgt ggggatcgga
      961 ccggtggacg ccgccctgaa cgccatccgg accgcggtca gcgagaacat atcgctggtg
     1021 gagtacaagc tgaacgccat caccggcggg agcgacgccc tgtgcgaggt caccgtgaag
     1081 atgaggatga acgacgacca gaaggtgatg tcggtgggca agagcatcgg ctcggacatc
     1141 gtgctcacca gcgtggacgc ggccatgggg gccatcgacc gcctgctggc caggcatagg
     1201 gagtaggagg aagcacatga gaacggaccc caagaccatt tcggagaaga tcctttcctt
     1261 gagatcgggg caggacgcta gcgccgggga catcgtcgag gcggacgtcg actacgtcat
     1321 ggtgaacgac gtgaccggtc ctctggcctt ccaggagttc gaggccctgg agtgtgaacc
     1381 ggtgcgcgac cggatagtgc tcatccccga ccatttcgtc cccaacaagg acgtggcctc
     1441 ggccaagcag gccaaggaga tgagggagtt cgcaaagcgc tgggggatca ggaactactt
     1501 cgaggtgggc cgcggagggg tctgccacca ggtcatgctg gacaacgggt tcgcctatcc
     1561 gggcgcgctc gtcgtgggcg ccgattcgca cacctgcacc tatggagggg tgaacgcctt
     1621 cgccacaggg gtgggcagca ccgaggccgc ggcggtgttc gcgaccggcc atctatggtt
     1681 caaggtgccc agttccatca gggtcctgct caaggggagg ccctccaggt acatcggcgg
     1741 gaaggacctc gcgctcaagc tcatcagcga catcggggtg gacggggcca cgtacaaagc
     1801 gctcgagttc ggggggaccg gggtctcggc cctgacggtc tccgaccggc tttcggtggc
     1861 caacatggcc atcgaggcgg gggggaaggc cggcatattc ccgtgcgacg cggccaccgt
     1921 ggcctatatg aagacggtgc ggaagggcgg cttcaaggcc gcgctccccg atgagggcgc
     1981 ggtctacgag cgcaccctgg agtatgacct gggggaattg gagagcatgg tggcgcttcc
     2041 ccatctgcca tccaacggca gggctgccag ggacgtcaac gttccgatcg accaggcgta
     2101 cctgggctcc tgcaccaacg gcaggataga ggacctgagg accgccgccg aggtcatgcg
     2161 cggaaggaag gtccacccgg acgtacgcat ggtcgtcgtc ccagcgtcgg tgagggtctt
     2221 cgagcaggcc ctggccgaag ggctgctgac ggagctctcg aaggccgggg cgttcatctc
     2281 cgggccaacg tgcggcgcct gcctgggagg gcacatgggc atcctcgccc cgggggagag
     2341 gtgcgtgagc agcaccaacc gcaacttcat cggacggatg gggcatcggg actcggaggt
     2401 ctatctggcc gggccggctg tggtggccgc ctcggcggtc accggacgga tcaccgaccc
     2461 gagggacctg gaggcggtcc gatgagggag gtcaaggggc atgtctggag gttcggcgac
     2521 cacgtggaca cggatcagat cattccggcc gaaaggctga cgagcgagaa caacgccatc
     2581 ctcggcacgt tcgcgttcga gaaggtccgg ccgggaatgg ccgagcaggt caagcagggc
     2641 gatgtcatcg tggcgggccg caacttcggc tgcggttcga gcagggagca cgcgccccgc
     2701 gccctgctgc agatgggcat agcctgcgtg gtggcggaga gcttcgcccg gatattctat
     2761 cgcaattgca tcaacaccgg gctgctcccg gtggagtgca ggatcgaggc cgatgacggc
     2821 gacctgctgt cggtggacct tgccaaaggg acgatagtca atcacaccaa agggaaggaa
     2881 tggggcttca cccccttccc cagcttcatc caggagctaa tggacaaggg cggcctcatg
     2941 gccaagatca aggaggagaa aagatgcaca agatagctgt actgccaggg gacggtatcg
     3001 gcccggaggt cgtagccgag ggcctgaagg ttctgaacgc cctgtccgag aactactcgg
     3061 tcaagttcga cttccaggag ttcgatatca gctcggagcg ttaccttcgc accgggaaac
     3121 tggtaacggc cgaggacatg gaccagctca ggaagttcga cagcatcttc ctcggggcga
     3181 tcggggacga cagggtaaag ccagggatcc tggagaaggg catcctgctc gccctccgct
     3241 tcggcttcga ccaatacatc aatcacaggc ccgcccccct ctggaggccg ttcgggcgcc
     3301 taaagaggga cgtggacttc aacatcgatg tgttcaggga gaacaccgag gactactaca
     3361 tcggcgccgg aggacgggtg aacaatggga gggggtccct ggaccttcac gtgaaacggc
     3421 atctgtatga catgaaggtg aggctggagg ccaacgccga cgctgcggac gactatgcct
     3481 tcgagatcgg aatgatgtcc cgaaagaaca tcgaacgatt cgcggaccac gtcatagctt
     3541 tctcgaatat catcggggag aactacatca ccgtcgtgga taaggcgaac gtgtgcagca
     3601 acatctattc cctgtggagg gaggtgtggg acgagaagtg caaaaaggcc gggaagggac
     3661 atggttacat gtacgtcgac gccatgacca tggccctggt gaagaaccct gacaaattca
     3721 gggtgatcgc cgtccccaac atgttcgggg acataatcac cgacctgctg gccgaggtga
     3781 cgggcgggct gggcctcgcc ccgggcggca acatcaatcc cgaaggcatc tccatgttcg
     3841 agccggtgca cggctccgcg ccgaagtaca agggcatgaa ccgcatcaac ccggtggcca
     3901 cgatactcgc cgcgaagatg atgctggaca gcctgggcca cagggacctg gggcaggtcg
     3961 tgcaacaggc ggtgcgcacc gccttcgacc agggcgtgta cactcaggac ctgggcggga
     4021 aggccaccac aacggagatg ggggacgcgg tcgtggaggc catacgcaag gcgaagtgag
     4081 cgtctcgccc ctgtcccccc cccccgaaat gctgagcggg acgggcaccg cgcccctctc
     4141 catttcatca tgggggatga ccgtacacgc gcccgaccat ttcctcgatg cggccgccgt
     4201 aggggcgcca gtgaagccgt cccgaggtca gcgccgcccg gccacttcga ggtacagctc
     4261 ctcggtccgg tcagcgatct cgtcccaggt gaaggcgcgc atcctttccc tggccgccct
     4321 tccaagtctg tcccttcctt cttggtcgcc atccagggcg ttgatcgccc tcgccagtcc
     4381 ctccacgtcc cccgtcggga cgaggacccc tgcgtccccg accacctccg gcagcccgcc
     4441 ggtgtccgag gcgatcacag gcttcccgta ggccatggcc tccgcggcca ccatcccata
     4501 cgcctcgtgc agggagggga tgacgaacat gcggcactcg ctgagcaacc tgtgcttctc
     4561 cgcctcgctg acccagccca ggaaatcgat ccgatccgtg agcatgaggc ggtgcgatag
     4621 cccctccagc cgcttccgct ccggcccctc cccgcagatc ttgagctggc atctggtatg
     4681 cttcatcgcg cggatcagga cgtccaaccc cttcaggcgg accagccttc ccagggaaag
     4741 gatgaagtcc ccgtccttca ccggcccctg gggcagctcc acgccattgt agatggtggt
     4801 gagctgcctg tcggggaacc cccgggcggc cacgtccctc cgcatgtagt ccgagacgca
     4861 gacgatccgg tcgaaagcgt gaaggtggcg aaggaagagc cggtcgttca gctcgctggc
     4921 gtaatgggtg aggccgaccc cctcccccag ggtattgtgg cacgtgtaga ccttgggccc
     4981 cgggaacgcc aggaccccct tggtgagcga accggcccac ctgtaatgga ggtccacgat
     5041 gtccggggcc aacccccgga atgcctcctt caccttggtg atatgaacat aggggggatt
     5101 gtagacgtcg atgaaccggg aggggagtcg cacgacggtg tacccgtcca tctcctccct
     5161 ctccgccgtg ccggggaggc ggctggtgag gacgaacacc tcatgccttc ttcccaaccg
     5221 gcggcacaca tggtgcagcc ggttctctat ccccccgacg aatgggtagt ggaaggcatt
     5281 cgcctcgacg atgcgcacct tcccgccctc acatgtccac ccggcggctc agctcgctgg
     5341 gcaggtacat tggacggaac ggcatgtccc cgatctcgcc gcgcacccgt tctatcaact
     5401 ggtccacggt catctccacg ttcctgttct gcgagcggtc gtagacagtc aggacgtcgg
     5461 agcccatctc cttgtcgccg atcacgatcg cataccccac ccagtcctgc ttggcctccc
     5521 ggaccttctt gccgacggtc tcggagcggt cgtccagccc gacccggacg ttggccctcc
     5581 ggaccctatc ggcaagctcc ctggcccttt ccagatgggc ctcggagacg ttcatgaagc
     5641 gcacctgctc cgggttcacc caggtcggaa gcgtgcccac cttcccccgg gtctccatct
     5701 gcaccgctgt gtcgaacagc gtgtacaggt agcgctccac ggtcccgatg accgcggtat
     5761 gcaggatgac cgggaacgtc ttcttgcccg attcgtcggt gtacgcgatc ccgaagcgct
     5821 gggcgttgcc gatgtcgatc tgcaccgtcc cgatctccct ggccctcttc atgtcgtcca
     5881 ggatatggta ctcgatgttg accgtccaat agtagttgat gccttccgga tagaagtgca
     5941 ggagggccgg cctggcatgc ttgcgcacga tctccatgat caggcccttg tgcttttgat
     6001 aggcgtccca ggaggagaag ttgatcagca tctcgtagtc cctccccagg gcatttgcct
     6061 catcgaatat cctcccgtcg aggcgggaga accactcctc ggcctccggg atgtcccggc
     6121 agacgacatg cagatcgggc atgttcatgc ggcgggtgcg gaagcagagc atggtctccc
     6181 cggactgctc catgcggtac gagtccgcca cctcgaaggc cccgaacggc aggttcttgt
     6241 agctgatgtt ccagaggcgc atcatggcga actgctggtg gcaggcggcg tacctcatca
     6301 caaagtcccg cttctcgccc ttgatgacgt acagcctgtc gccgaacagc ttggcgtgct
     6361 cggccaccgg cccctcgtcc aggctgaaca tgttcgtgcc ccggacggtg tagaccggga
     6421 gggagaggga gttcacgatg cgcgctgagt aatcggccac caggtcgaac atcaacgccc
     6481 ccttggggga gaagcacatg tggccagcat cgctcatgct ctcccagttg atcccgaact
     6541 tcttgcagag ccgggtgtac ttgacctccc cggtggatgg ccactccttc ttcagcgcct
     6601 ccttggccat catgatctgg aagcattcgc tgcccccctc gaagcccctg gggtccacct
     6661 cgctcccgtc cggcaggagg acgaggaagc gttcctcccc cttcggggca ggcggggcct
     6721 cctcccccac tatctcccgg gagagctccg agagcggatg gcccttgcag ctgatcctga
     6781 acgccttgta ccacccgaag ggggcacggt gcacctgcca tcccttcgcg gcgatcatcc
     6841 tctcgatctc acggagcacc ctcttcccgg tctccgcgtc cgccaggtcg gggctaaggt
     6901 gggcgtaggg atagagcatg atgcgctccg cccccacctt ctcggccgtc cctatgatgt
     6961 ccgccgatgc ctgggaggcc accgcctcca ccttgtcctg gtcgccgcgc tcgacggtga
     7021 ggaaggccac cagcacctcc tccatccggc cggtcctctc ctggtcggcc acggcctcgg
     7081 ccatcggcgt gggcttcttg acctcgtact ccatgaaatc ggcgtgtatg tagagcgttc
     7141 tcatcgtatc gcctgttagg gttgctcagt aatggtccac gtgtataaat aagcgctttc
     7201 cggcggaagg cctttgccag atggtccgag ggcctttccg ggaatgggca atgtttaaat
     7261 tcaaggaaag gtttgcctgc tcagaatcct tatgaggtcc tcccggatag ccagggacat
     7321 cggaggggct acgaatgtcc tgctcatcgt tctgatcgcc gtcctcatgg tcagcgccag
     7381 tacggtagcg ataatgttgt accagggggg ccaatccgtc gaggccgccg agaacgtggt
     7441 gaccgtcggc cagaccatca aggtcgacta catcggcaag ctgctggacg aaagggtttt
     7501 cgacacctcc ctctactccg tggcgagcaa tgacgccgcc tatcccaaga ccctgtcgtt
     7561 caccctcaag gccaatacgt cctatgtgcc gctcaccttc aaggtggggg ccgggacgat
     7621 gatccccggg ttcgaaacgg cggtcgtggg catgagggta ggggagacca agacggtcac
     7681 ccttcccccg gaggaggcgt acggtcccat ggaccagagc aagctggtga cgttccagct
     7741 cactgaggcc gtgccccttg tgcgcatatt caatgccagc accttcaagg ccgagtacgg
     7801 ggaggacgcc gcatccggcc tgacggtcta cgaccccgta tacggatggg cagcgacggt
     7861 catggagtac aatgcttgga cggaccgggt gacggtgaag aacgcgccca ccctcaacgc
     7921 cctctaccat atctacggga cggggagcgt ggggtgggac gtaaaggtca taggcatgga
     7981 cagcaactcc aaccgcatca ccatccagca tcagctgggc gaaggggaca gcgacagggt
     8041 caagggcctg gacggcagca gcaccttctt catcatcgac gtcgatgagg agaagggcac
     8101 ggccgtgcgc aactacaact ccgaactcgc cggcaagacc ctggtcttca ccataaccat
     8161 agtggccata gttgagtagg cccggggacc tcagcgtggt cccccatcct cgcctgtacc
     8221 agccacgtcc aagcggtcga cctttctctt gcggtaggtg gaggcgatga agctcagcac
     8281 gattattatg attaccatcg cgagggtcac gtccgccgcg agcccccttt ccatatacgc
     8341 caggaaataa ttgctggccg caaggatgga cccgtacttc agcgtccccc ctaggacctg
     8401 atagaagatc gaccttctgt aggaccagcc gctgagggcc acgaaggccc ccgtgaaggg
     8461 caggatgggc gcgatccggt tgagcaggat catgcgctcg tctggaatga gcaggaacct
     8521 gcggtagcgg tccacgactg tggctatttt cggaggcacc ctcaggcgct ttaccaccat
     8581 gtacagggtg gtgagcccca ggacctcggc caccgctatg gtgacgacga acatggacca
     8641 cagggcccaa tccgggaccg tcccgtagcc agacatgaag aacagcacca cgaacagctc
     8701 cggcagcgtc gggaagacga tggcgtcgat gtaaaagagc aggaaggtgc acaccatcca
     8761 tccggccagg cccagcggcg agaacagcac gtagaatgcc tctccgatgg agaacatccc
     8821 atctcccaag cgctagcgct ttaaaaatgc tttgtcattg gcccggccag ccttggcctc
     8881 gatggcgtac ctgtacacgt ccacgagcct ggtggcccac tgccccgcgg agaactccct
     8941 cgccttggcc agggcgccct cccgatagcg ctcgggtccc tcagcaaccg aagcgtggcc
     9001 tgggaccagc tgtatgg
//
LOCUS       DAOSYN010000033         8866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_6714_length_8866_cov_3.976734, whole genome shotgun sequence.
ACCESSION   DAOSYN010000033 DAOSYN010000000
VERSION     DAOSYN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6714_length_8866_cov_3.976734"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLJ11_02560"
     CDS             complement(<1..732)
                     /locus_tag="PLJ11_02560"
                     /inference="COORDINATES: protein motif:HMM:NF021571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2299 family protein"
                     /protein_id="HOO03581.1"
                     /translation="MPSANQDQALQHLKQWADRTGYQVGPIEGTGNDFILEVTERDTF
                     PSVQFIHQRKEQPYLLVVGQVKVPQEDRQLLKRALGEGFKRFIWDLKLVLLDKGVDFI
                     VIGEDEGDPDAWEVHLRIYVKEFDPNLLQGAYTRVKNSLIAVIWSYKRALDQAAMFSA
                     GPSEQFPLPADDVDMDVAEDPGRSRRIMNVMANTGIIMMVTLVDGFAQTMMDVAGAMA
                     EGMTEAFEGEEAAEQVAREIEERTPE"
     gene            complement(828..1958)
                     /locus_tag="PLJ11_02565"
     CDS             complement(828..1958)
                     /locus_tag="PLJ11_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOO03582.1"
                     /translation="MTENDMRDVIRGLRDVAAAETRISFVDPNGHLYYLGYDIDDLVG
                     RVVYAEVAYLLVHKRLPNRAELDRFNSELIGHMHLPDEVEEGIKRSPAAAHPMDILRT
                     GVSRLGEHDREALDLSQEANLRKATRLIAQVPTIVATIHRSRQGKDVLEPRDDLSFAQ
                     NFLYMFKGSVPERDEADVMHRIMVLHADHGFNASTFAARVTASTRSDMYSAVTTAIGT
                     LRGPLHGGASQKVMEMLDDIGPEGIEEYIEGLLDNGKKIMGFGHRVYKVEDPRTKHLR
                     SIVQNLCDRDRTRELYDKCLRIEGIVHARKRIYPNLDFYSAVAMDAIGVPKEYFTPFF
                     ASSRIVGWVAHIMEQYEDAVLLRPASKYTGEFGRPFVPIDRR"
     gene            2445..3632
                     /locus_tag="PLJ11_02570"
     CDS             2445..3632
                     /locus_tag="PLJ11_02570"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO03583.1"
                     /translation="MASRDRTSGRFLLVLGTIAALAIASSTMAKNPALPLLALHIGAD
                     DAQIGLISAVSPIPGILISGIAGAYSDKHGRARVITLSLLIFASAPFLYLFITNVWQL
                     ALVRFYHGFATAMFMPVATAAVADRFAPAKRGEALGTFSSFSMVGRFSAPFLGGALLY
                     YTTFSSLYLTIGVSAAIALALSFIVPWDEGGTGVQAKKFEGTMVAALRNVLEERRIML
                     TSSMEAVQYFAMGAFETFLPVYMDQELGLTALEIGTVMGLQVVAMLLAKPFLGRLSDR
                     WGRVPFIVTGLMLGAITIVLMPWTDDGVVLSMLSIGFGLTVATVTASTSALVTEIAGT
                     SAHGSSIGVLSSIMDVGHSIGPFMTGIVVGTFSFTAGFGLAGALLTMGAIVFAVGMRG
                     HRK"
     gene            complement(4077..4460)
                     /locus_tag="PLJ11_02575"
     CDS             complement(4077..4460)
                     /locus_tag="PLJ11_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03584.1"
                     /translation="MEGKEKDTAAANEAADKVVALKVLQRIYSDQLNEYERQMDEVSA
                     LGRVNRNTLYNKAVLHEIEGVAQRRGFEELLSAVWVAQSMLDDIVALQRTVCVLKRSM
                     DEGALATVEREERGIDHESLPYETI"
     gene            4598..5755
                     /locus_tag="PLJ11_02580"
     CDS             4598..5755
                     /locus_tag="PLJ11_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOO03585.1"
                     /translation="MPIDFSKRSQLIVQSEIRNMTLECNRIGGINLAQGVCDTDTPRE
                     VVEGAYQAAVDGVNAYTRYDGLPVLRTAIAKKFKEFNGIDIDPEGEVVCSAGATGAFY
                     SACMALLNEGDEVLVLEPYYGYHVNTLLAMGAVPRFVSMHPPEWRLDLEAVAAAIGPR
                     TRALVVNTPANPSGKVFSREELRGLVDLAVDHDLLILSDEIYEYFVYDGQRHVSPMAF
                     PEARDRTVVISGYSKTFSITGWRVGYAVCERRLARMIGYVNDLVYVCAPAPLQVGVAK
                     GIALLPGEFYRRLCLQYEKKRDMMVSGLREGGLRPHVPQGAYYILADVSSVPGGNAKE
                     RALHILRECGVAGVPGSAFYHQGGEDTVRFCFAKRDEVLRTACDRLSLLRW"
     gene            complement(5738..6613)
                     /locus_tag="PLJ11_02585"
     CDS             complement(5738..6613)
                     /locus_tag="PLJ11_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOO03586.1"
                     /translation="MQKRQVARLAVVVGLLIFLIKLTAYAISGSVALLSDALESIVNI
                     AASVMMTASIKVASMPADADHKYGHQRAENISSMVEGMLIVIAAILIIMASLMRLLDP
                     QPFTSIGLALIVSLVATAMNGGMSYLLMRTSRISGSIALEGDAKHLLSDVISSVGIVI
                     GLSLAELTGWYILDPILALIVAVLLIRMAYSVLTKTLSDLMDQRCPESEALIESVLKE
                     QGGFLQFHDLKTRRVGGNVYAEMHICVPAESTMAEAHELTERLEEALRAKVPGIIVLI
                     HVETEKELVGSPPEE"
     gene            complement(6666..6905)
                     /locus_tag="PLJ11_02590"
     CDS             complement(6666..6905)
                     /locus_tag="PLJ11_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03587.1"
                     /translation="MSIMKLSRTKNKGGKAATQVDIMLETGEVLLSVNIRPSKVLYTE
                     DKSYLTFFVRHDPKLTEAEIDALFERALAESVGKG"
     gene            7106..7393
                     /locus_tag="PLJ11_02595"
     CDS             7106..7393
                     /locus_tag="PLJ11_02595"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOO03588.1"
                     /translation="MVSIKDVLLKSVLTSDAYNIGTVNDFELSVEDWKLTHIGVTLNM
                     QSKKDLGIKKFSLLGPTLCIPIDHVDKVGDVVTLKITFDQLKEIPECKKLM"
     gene            7475..8638
                     /locus_tag="PLJ11_02600"
     CDS             7475..8638
                     /locus_tag="PLJ11_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO03589.1"
                     /translation="MSQGPVNAKLIYKFFEAANMQRWNDHIRPVELTELDKQSHKMVI
                     AYVLARFEETEKGTHIDWPKLIEGGIFEFLHRLFLTDLKPPVFHHLQREKGKELNEYV
                     FRELVSEVPDIDNEFLERFKAYHSRDEESIERRVLRAAHYLATNWEFHLIYSANPTML
                     GIEETKRSIEMQIEDHYDLIGVQRIMLAKKSYGFIEFCGQLRYQQRWARAPRIPRTSV
                     LGHMLMVAIMSYLCSLRINAPPERLVRNFYTALFHDLPEVLTKDIITPVKRSVGGLEE
                     LISDYEQQQVMEKLLPLLPAPWRRDFEQLLVDPFRTRANDNEGGWVVDGAMLKGCDNL
                     AAFIEASSSIKYGVSSKVLRVGKQRLMEIYHNGGMVAGIDFYQLMLEMDHMEI"
     gene            complement(8639..>8866)
                     /locus_tag="PLJ11_02605"
     CDS             complement(8639..>8866)
                     /locus_tag="PLJ11_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03590.1"
                     /translation="RFYEMAPGQVLAESPDNQAVDVSDVLSVHLSYDGEGTGAYVARF
                     RLEEGDLTVTVPYERYYRDLLIRMFEGRISW"
BASE COUNT         1687 a         2675 c         2626 g         1878 t
ORIGIN      
        1 ctccggcgtc cgctcctcga tttccctggc cacctgttcc gcggcctctt cgccttcgaa
       61 agcctcggtc ataccctcgg ccatggcccc ggcgacatcc atcatcgtct gggcgaagcc
      121 gtccacgagg gtgaccatca tgatgatccc ggtgttggcc atgacgttca ttatccggcg
      181 ggagcgccct gggtcctcgg cgacgtccat gtcgacgtcg tcggcgggga gcgggaactg
      241 ctcagaagga ccggcggaga acatagccgc ctggtccagc gccctcttgt aggaccagat
      301 gaccgctatc aaggagttct tcaccctggt gtaggcaccc tgaagaagat tgggatcgaa
      361 ctccttcacg tagatcctga gatgtacctc ccaggcgtcc gggtcgccct cgtcctcgcc
      421 aatgacgatg aagtccaccc ctttgtccag cagcaccagc ttgaggtccc agatgaatcg
      481 cttgaacccc tcgcccaaag ctcgtttcaa tagctggcgg tcctcctggg ggaccttgac
      541 ctggccgacc accagtagat aaggctgttc cttgcgctga tggatgaact gcacgctcgg
      601 gaaggtgtcc ctttccgtca cctcgagaat gaaatcgttc cccgtcccct cgatcggacc
      661 gacctggtac ccggtgcggt ccgcccactg cttcagatgt tgaagcgcct ggtcctggtt
      721 ggcggatggc atggcatcga cctcgaccaa agaatggtca ttatcgattc catttacgga
      781 ttaataaagg ggttgccctg acccttcggt tcacccgggg gcggaactca ccttctatcg
      841 attggcacga agggcctgcc gaactcccca gtatacttcg atgcggggcg gagcagcacc
      901 gcgtcctcgt attgctccat gatatgggca acccatccca cgatcctgct ggaggcgaag
      961 aagggggtga agtactcctt cggtacgccg atcgcgtcca tggccacggc ggaatagaag
     1021 tccaggttgg gatagatccg cttgcgggca tggactatac cctctattct gaggcacttg
     1081 tcgtatagct ccctggtcct atccctgtcg catagattct gcactatgct tcgcagatgt
     1141 ttggtgcggg ggtcctcgac cttgtacacc ctgtgcccga accccattat cttcttgcca
     1201 ttgtccaata ggccctcgat atactcctcg atcccctccg gaccgatatc gtccagcatc
     1261 tccatgacct tctggctggc tccgccgtgg agggggcccc tcagggttcc gatggcggtg
     1321 gtgaccgccg agtacatgtc cgaacgggtg cttgcggtga ccctcgcggc gaaggtggag
     1381 gcgttgaagc catggtccgc atgcaggacc atgatccgat gcatgacatc ggcctcatcc
     1441 ctttccggaa cgcttccctt gaacatgtac aggaagttct gggcgaaact gagatcgtcc
     1501 ctgggctcga gaacgtcctt cccctgccgg gatcgatgaa tggtggcaac gatagtcggt
     1561 acctgggcta tgagcctggt ggccttgcgg aggttggcct cctgtgagag atcgagcgcc
     1621 tcccggtcat gttcccccag gcgggagacc ccggtgcgca ggatgtccat cggatgtgcg
     1681 gccgcagggc tgcgcttgat gccctcctcc acctcatcgg ggagatgcat atggccgatg
     1741 agctcggagt tgaaccggtc cagctctgcc ctgtttggca atcttttgtg aacgagcagg
     1801 tatgcaacct cggcatacac cacccggccc actaggtcgt cgatgtcata cccgagatag
     1861 tacaggtgcc cgttggggtc cacgaagctg atccttgtct ccgcggcggc cacatccctc
     1921 aatccccgta tcacatctct catgtcgttc tcggtcatta tgaaccatct atccggaccc
     1981 tacgatccca ggtgccgcgg atgggaagga tcgcaggctc gctacggttg cctttaaaac
     2041 atcatctcat aaaacccttt ttatcatcgt ccagccccat agttcgacag gtcgatacgg
     2101 ttgatgagct ggtctgaggc cgcttcatgc gggctgttcg gatacccctc tcccatgaat
     2161 cggctgatcc gtggccattt tccttcgtcc aggctgaaag agggttgcgg ttccccgccg
     2221 ccatgccgtg cagcccgaca tgcggggtca ccgccacctt tgccgctgag agcatggcga
     2281 taccgacact aacgacccct ggactaggca ggatcgctgt cccgtacgat gcccggtgca
     2341 tcgatggtgc cctgtgagct gcggtccgct tagccagtgc ccaaaggctg gccgtgagcg
     2401 gacgcatgga aatcaattaa tatcgatagc ctccatccag ccctgtggct tcccgcgata
     2461 ggacgtctgg tcgctttctc ctggtcctcg ggaccatagc cgcactcgcc atcgcgtcct
     2521 cgaccatggc caagaacccc gccctgccgc tgctcgcgct gcacatcgga gcggacgacg
     2581 ctcagatagg gctcatctcc gcggtaagcc cgatcccggg catcctgatc agcgggatcg
     2641 cgggcgcgta ctcggacaaa catggaaggg cgagggtcat caccctctcc ctcctcatat
     2701 tcgcatcggc gccatttctt taccttttca taacgaacgt ctggcaattg gccctggtcc
     2761 ggttctacca tggctttgcg accgcgatgt tcatgcccgt ggcaaccgcc gcagtggcgg
     2821 accgcttcgc ccccgccaag agaggcgagg cattaggaac gttctcctca ttctccatgg
     2881 tcggaaggtt ctcagcccct ttcctcggag gtgcgttgct ctactacacg accttctcct
     2941 ccctatacct gaccataggc gtgtccgcgg cgatcgccct cgccctttcg tttatcgtcc
     3001 catgggatga gggtgggacg ggcgtacagg cgaagaagtt cgaggggacg atggtggccg
     3061 ccctcaggaa cgtgctcgag gagcgccgga taatgctcac atcctcgatg gaggccgtcc
     3121 aatactttgc catgggcgcc ttcgagacgt tcctgccggt ctatatggac caggaactcg
     3181 gcctcaccgc gctcgagatc gggaccgtta tggggctcca ggtggtggca atgctgttgg
     3241 cgaagccatt cttggggcga ctgtccgaca ggtggggaag ggtccccttc atcgtcactg
     3301 gcctcatgct cggagcgatc accatagtcc tcatgccgtg gacggacgat ggcgtggtac
     3361 tctccatgct ctcgatcgga ttcggcctta ctgtggcaac ggtcacggct tcgacctccg
     3421 ccctggtgac cgagatcgcc gggacgtcgg cccacgggtc ctcgatcggg gtcctgagct
     3481 ccatcatgga cgttgggcac tcgatcggcc cgttcatgac aggcattgtg gtgggaacgt
     3541 tttcgttcac ggccggcttc ggcctggcgg gggcgttgct gacaatgggc gccatcgtct
     3601 tcgccgtcgg gatgcgcgga catcgcaaat gaccttcaag ggggagaagg aagccgtacc
     3661 ccgctctttc ctttggatcg tttcgccccg tccaacgagg ttggggtgag gggaccggga
     3721 ccacctctcc ctatcttaag gccgatgtcg tggtctccgg ggaacggaag cgatcgagat
     3781 ggtgggtgag tggtaccttc gcctctatcg ccctcactgc ttctcagagc atcgtccatc
     3841 ggtgccgggg ggcgtgaacg gcccccacgc tgcccggtcg gatacgcggg gcaaaggccc
     3901 tggatttcaa tggcctccct tcaaacgggc atccgctgtc aaatgggagg ctgggaaggg
     3961 ggatgatatc cgtcctcccc tttccatcgt gctgatccta gatgagcgac ggtgacattg
     4021 ggcgttaacg ggtaggtggc gctttgaggc catcagggat atggcaaccc gatggctcat
     4081 atggtctcgt acggcaggct ctcatggtcg atgcccctct cctccctttc gaccgtggcc
     4141 aaggccccct cgtccatgga gcgcttcagc acgcagaccg tgcgttgaag agcgacgata
     4201 tcatccagca tggactgggc gacccaaacg gcggaaagga gttcctcgaa ccccctcctt
     4261 tgggccacgc cctctatctc gtgcaggacc gccttgttgt agagcgtgtt gcggttgacc
     4321 cttcccagcg ccgagacctc gtccatctgc ctctcgtact cgttgagctg atcggaatag
     4381 atcctctgta gaaccttgag ggccacgacc ttgtcggcgg cctcgtttgc agctgctgta
     4441 tccttctcct tcccttccac gtgatcaccc cggggcgaag cgctttgcta gcgcgcaatg
     4501 gggccaacat cgttcaacgt cattagcctt tcctggactg ggcggacgga atcggatatg
     4561 gttatagctt ccttggtgac tattcgtccc tgatacaatg cccatcgatt tcagcaagcg
     4621 gtcccagctc atcgtccagt ccgagatcag gaacatgact ttggagtgta accgtatcgg
     4681 agggatcaac cttgcccaag gagtgtgcga caccgatacg ccgagggagg tggtggaagg
     4741 ggcctaccag gcagcggtgg acggggtgaa cgcctacacc cggtacgatg ggctgccggt
     4801 cctcaggact gccatcgcca agaagttcaa ggagtttaac ggcatcgata tcgatcctga
     4861 gggagaggtc gtctgctccg ccggagccac gggggccttc tacagcgcct gcatggcctt
     4921 gctgaacgag ggcgatgagg tgctcgtcct agagccctac tacggctatc atgtgaatac
     4981 cctcctggcc atgggcgccg tcccccgctt cgtgagcatg caccctccgg aatggaggct
     5041 tgacctggag gcggtcgcgg ccgccatagg gccgcgcacc agggccttgg tcgtgaacac
     5101 tcctgcgaac ccatccggga aggtcttttc ccgggaggaa ctcagggggc tggtggacct
     5161 ggcggtggac catgacctcc tcatcctctc cgacgagata tacgagtact tcgtctatga
     5221 cggccaaaga cacgtttccc cgatggcctt ccccgaggcc agggacagga cggtcgtcat
     5281 ctccgggtat tccaagacgt tcagcatcac cggctggcgc gtcgggtatg ctgtgtgcga
     5341 gcgaagattg gcccgaatga tcggttatgt gaacgacctc gtatacgtct gcgccccggc
     5401 ccccctgcag gtcggtgtgg ccaagggcat cgcccttctt cccggcgagt tctaccgcag
     5461 gctctgcctg cagtatgaga agaaaaggga tatgatggtg agcgggctga gggagggggg
     5521 cctccgcccc cacgtgcccc agggcgcgta ttacatattg gcggacgtaa gctccgtgcc
     5581 cggtgggaac gccaaggaga gggcgctcca catcctgagg gagtgcggtg tggccggcgt
     5641 ccccggctcg gccttctatc atcaaggggg agaggacacg gtcaggttct gctttgcgaa
     5701 gagggacgaa gtgctgagaa cggcgtgcga tcggctctca ctcctcaggt ggtgagccga
     5761 ccagttcctt ctccgtctcc acatggataa ggacgattat cccggggacc ttggcacgca
     5821 gcgcctcctc caatctctcc gtcagctcgt gcgcctcggc catcgtgctc tccgcgggta
     5881 cgcagatatg catctcggcg tagacgttgc cccccaccct gcgggtcttc aggtcgtgga
     5941 actgcaggaa gcccccctgc tccttgagca ctgattcgat cagcgcctcc gattcagggc
     6001 accgttggtc catcaggtcg gacagcgtct tggtcaggac cgaataggcc atgcgtatca
     6061 ggagcaccgc cacgatcaga gccaggatgg ggtccaatat gtaccagccg gtgagttcgg
     6121 ccagggaaag cccgatgact atgccgaccg aggagatgac gtcggacaga aggtgcttcg
     6181 cgtccccctc cagagcgatc gagccgctga tgcgggaggt ccgcatgagc aggtaggaca
     6241 tccctccgtt catggccgtg gccaccaggg acactattag agcaaggcct atggaggtga
     6301 agggttgagg gtctaaaagg cgcatgaggc ttgccatgat tatgaggatg gccgcgatca
     6361 cgatgagcat cccctccacc atgctggaga tgttctccgc gcgctgatgg ccgtacttgt
     6421 ggtccgcatc ggcgggcatc gatgccacct ttatcgaggc cgtcatcatc acggaggccg
     6481 cgatgttgac gatagattcc agcgcatccg acagcagggc gaccgagccc gatatggcgt
     6541 aggccgtcag cttgatcaag aagatgagca gccctacgac cacggccaat ctggcgacct
     6601 gcctcttttg cacgtcatag ggcaaagcga agggccgata aacccttttt catgtagagc
     6661 gggggtcagc ccttccccac gctctctgcc agggccctct cgaagagggc gtcgatctcc
     6721 gcctctgtga gcttgggatc gtgcctgacg aagaacgtca gatagctctt gtcctcggta
     6781 tacaatacct tggagggacg gatgttcacg ctcaatagca cctcccccgt ctccagcatg
     6841 atatcgacct gggtggcggc cttcccgccc ttgttcttgg tccgggagag tttcatgatg
     6901 ctcattcggt tcgcctggta tagacggcgc atccgttcat ccaatcctgg gccttccacg
     6961 gcctggaatc ggaattggat atgaaaagtt tgcccatcat cgttagatgc tgagaaggag
     7021 ctggatccaa ttcatgtcac ttcatatttg tgaaaaggga agcgaaagtg atttatggcg
     7081 ttcgttcgct actgttttgg tcataatggt cagcatcaag gacgttctct tgaaatcggt
     7141 attgacctcg gacgcctaca atatcggaac ggtgaacgat ttcgaacttt cggtcgagga
     7201 ttggaagcta actcatatcg gcgtgacctt gaacatgcag tccaagaagg acctgggcat
     7261 caaaaagttc tccctgctcg gaccaaccct ctgcatcccc atcgaccatg tggacaaggt
     7321 gggggacgtg gtcaccttga agatcacgtt cgatcaactg aaggagatcc ccgagtgcaa
     7381 gaagctgatg tgagttcctt ctgccacgat cggtcacggc gcgcaccgca ggcgaagcct
     7441 taatcccgtt gccgtacatt cctggaagag cgccatgtcc caaggaccag tgaacgccaa
     7501 gttgatctac aagttcttcg aggcggccaa catgcagcgg tggaacgacc atatccgccc
     7561 cgtcgaactg accgagctgg acaagcagtc gcacaagatg gtcatcgcgt acgtgctcgc
     7621 aaggttcgag gagacggaga agggcaccca tatcgactgg cccaagctga tcgagggcgg
     7681 gatcttcgag ttcctccacc gtctcttcct cacggacctg aagccgccgg tgttccatca
     7741 cctgcagcgg gagaagggga aggaactcaa cgagtacgtc ttccgggagc tggtctcgga
     7801 ggtgccggat atcgacaatg agttcctgga acggttcaag gcctatcatt cccgggatga
     7861 ggagagcatc gagcgccgcg tgctgagggc ggcacactac ctagcgacga actgggagtt
     7921 ccatttgatc tacagcgcta accctacgat gctcggcatc gaggaaacga agaggagcat
     7981 cgagatgcag atcgaggacc actacgatct catcggagtg cagcgcatca tgctggcaaa
     8041 gaaatcctac ggtttcatag agttctgcgg gcagctgcgc tatcagcagc gctgggcccg
     8101 ggctccgcgc atccccagga cctccgtcct cgggcacatg ctgatggtgg cgatcatgtc
     8161 ctatctctgc tcgttgcgca tcaacgcccc tcctgagcgt ttggtccgca acttctacac
     8221 ggccctcttc cacgatctgc cggaggtgct caccaaggac atcatcaccc ctgtgaagag
     8281 gtccgtaggg ggcttggagg agctcatttc ggactatgag cagcagcagg tgatggagaa
     8341 gctcctgcca ttgctcccgg cgccctggcg ccgcgacttc gagcagctgc tggtggaccc
     8401 cttccgcacc cgcgccaatg acaacgaggg aggatgggta gtggacgggg ccatgctgaa
     8461 ggggtgcgat aatctggccg ccttcatcga ggccagctca tccatcaagt acggtgtgag
     8521 ctccaaggtg ctgagggtgg ggaagcagcg tctgatggag atctatcata acggcggcat
     8581 ggtcgctgga atcgacttct atcagttgat gcttgagatg gaccacatgg agatatgatc
     8641 accagctgat gcgcccctcg aacatccgga tgaggaggtc ccggtagtac ctttcatagg
     8701 gaacggtgac cgtgagatcc ccttcttcca gccggaaacg ggccacgtac gccccggtac
     8761 cctctccgtc atatgaaagg tgcaccgaga ggacgtccga cacgtccacg gcctggttgt
     8821 cgggactttc agcgagcacc tggccagggg ccatctcata gaaccg
//
LOCUS       DAOSYN010000034         8773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_6808_length_8773_cov_4.317733, whole genome shotgun sequence.
ACCESSION   DAOSYN010000034 DAOSYN010000000
VERSION     DAOSYN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6808_length_8773_cov_4.317733"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLJ11_02610"
     CDS             complement(<1..528)
                     /locus_tag="PLJ11_02610"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HOO03591.1"
                     /translation="MNVAKRIQELKGQRKAVVLAHNYQSPEVQDLADHVGDSLGLAQI
                     AARTDAEVIVFCGVDFMAESAKILSPEKKVILPESEAMCPMSAMCTAMELALFKERHP
                     QAAVVAYVNTSAAVKALADVCCTSANAVEIVRRIPADREIIFVPDMSLGDYAARQTGR
                     RLILWQGYCPTHHRIL"
     gene            645..1589
                     /locus_tag="PLJ11_02615"
     CDS             645..1589
                     /locus_tag="PLJ11_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03592.1"
                     /translation="MKAPDLDWLLSGDPVLSALVKRDLMGDTSQMPAILTDRRIKEMV
                     RDIADWPWPPCVSHKSAGHPIHKFAFLADIGLELEGTKAGDLAWKVQNGMDDGLPRIT
                     VNIPEHYGGTGRPTMGWSLCDAPLLLHSLAQCFPGMDLAPGMSRLNDLARENGMPCAV
                     SLELGRFRGPGRKDDPCPIACLYALKAARAIEPGKNERLARTISASLLDLWSSSRERH
                     PYMFFMGTDFRKLKLPFVWYDVLSVTDALSECPWVLDDARFRDMLSVIGSKQDPEGRY
                     TPESVWTAWKGWDFTQKKEPSRWVTFAVERIHRRVGRG"
     gene            complement(1591..2202)
                     /locus_tag="PLJ11_02620"
     CDS             complement(1591..2202)
                     /locus_tag="PLJ11_02620"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOO03593.1"
                     /translation="MEQPKRYELPKLAYGYKDLAPFLSEEQLTVHHTKHHAAYVNAAN
                     AILDKLDKARAEGADLDAKGMLKDLSFQVGGHYLHSVFWETLCPAGKGGEPTGRLKEE
                     LVKEFGSIDRFKKEFSAAAASAEGSGWAVLVYCMALHKPLIMQVEKHNVNVIPGFRVL
                     MDLDVWEHAYYIDYKNLRPKYIEAFWNHVNWDAVGKKLDAILK"
     gene            complement(2299..3726)
                     /locus_tag="PLJ11_02625"
     CDS             complement(2299..3726)
                     /locus_tag="PLJ11_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOO03594.1"
                     /translation="MSEYRRQHPLLVLLDTGRGLISNIGVALVIAVSLPSSTFGGFVG
                     AFIVALLALFALSIVYNGLRWRFFFYRYEEGMVHIRQGILFKRERTIRRERVQSINVY
                     ANVVQQALGLRTLQIKTAGVGVDAEVNLRAVSKEEAERIRQHLNKEGEAREGGLEEVA
                     KALRALQGRDLWLAGLTSGRFMALFSVAAVALSQVIDYIPQTLIDQALARITGVPLLL
                     GLGIVLALFFATWALSTVVFALQFANFTVRRFEDRLELDWGVIKRSHVTVRLHRLQAV
                     VVQQGLLRQPLGLCTLLVEVAGGGAKEQEKVSVLYPMLRMREVEGFLRDLLPEYRLPS
                     GATRAPERARRRYLFRSTVPMALLVAVLLVASYHWNVPYGGAALLLLVPALLLGRSRH
                     SNAKFSIDGDQLTIGFRSLGLYQVLIGREHVQSLSLLANPFQRLGRLRTLSISLLSSP
                     AGKAYALKDLDASDAWAVREWYSMG"
     gene            complement(3719..4249)
                     /locus_tag="PLJ11_02630"
     CDS             complement(3719..4249)
                     /locus_tag="PLJ11_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOO03595.1"
                     /translation="MTPRSEEVLENIRERLRMENEPRERIDPRARTVWTISGAMGAAV
                     VMAIALAYWFAIPALDAPAWTGWLALALALGYAVWGIYMAPLLRTRFWRYEVREKEME
                     IQHGILVIKRHLIPMVRVQFVDTEYGPLMRYFGLTTLYVSTAATRFAIPALPKERAEA
                     LRGEIATLARVSEEDV"
     gene            4344..4730
                     /locus_tag="PLJ11_02635"
     CDS             4344..4730
                     /locus_tag="PLJ11_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HOO03596.1"
                     /translation="MTSRREELAAKYFNCTERERAVFEAGIKLGTIYHQFVGTPVAAA
                     NVEILEKAIEDGVRVQPFVKDVKVGISREALRRKKDEFDYQTLTGPMMKVELTVTVGG
                     TTVVAAMDYKDDLHYPLMYVRGIESK"
     gene            4871..5413
                     /locus_tag="PLJ11_02640"
     CDS             4871..5413
                     /locus_tag="PLJ11_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HOO03597.1"
                     /translation="MNNIKIGITGLPSAGKTNTLIQVIKMLEEEGYTVGGMITEPILD
                     DKKRNGLYVMDWMTKERGVLAATDIHSKFMVGKFGIDLDVLQEIGVQALVNACEKADI
                     IVIDEVGKIEVESERFRDAVKEALKTEKPMLLTLHKKSRNPLLQDIRRRDDVRILEVT
                     PINRNLLPYKIMKLMKGELL"
     gene            5410..6495
                     /locus_tag="PLJ11_02645"
     CDS             5410..6495
                     /locus_tag="PLJ11_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(26)-N(2))-dimethyltransferase"
                     /protein_id="HOO03598.1"
                     /translation="MSLEGTIIKEGSTRLLVPPLISERGPGRVEGKVFFNRQMAFNRD
                     VAVVMFRSVIQPRKCLDAMAATGARGVRICTEAPGEYRFLINDREPLAGEYIRENVRL
                     NSIGNAEVGTADLRCLLTREVFDYIDLDPFGTPIPFLPAALQGMTRKGVLAVTATDTA
                     SLCGTHPGKCRRRYMANSQRSPFMHESGLRILIGTIVRMAAQEDRGISPLLCYYADHY
                     FRLFLRVREGAAAADVALAQMGMMAYDRATGERRVGEGSMGPMWLGPLLDHGALAEAA
                     APEGLACPDRVERYLELWREELDVPFYYETDELAAMLGLSPPGRAELLGALGDRGRVS
                     RTTFSPTGFRTDRPLEEVLEAFRSACQ"
     gene            6541..7830
                     /locus_tag="PLJ11_02650"
     CDS             6541..7830
                     /locus_tag="PLJ11_02650"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOO03599.1"
                     /translation="MPSIAVIGAQWGDEGKGKITDYEAERADMVVRYQGGSNAGHTIK
                     VGDEVFALHILPSGIVRKGVVNVIGNGVIVDLISLDREIAEVEARGRSVEGLRISDRA
                     NIILNWHRLLDGAEERFRGGRTVGTTGKGIGPCYSDKIARSGIRMGDLLDPALLAERL
                     DTVMPMKLKVMEVLNVRLAQTRDELYSELLAYGKRYGRYITDTSVLVNEALDEGKNVL
                     FEGAQGTMLDIDHGTYPYVTSSNCIAGAICTGAGVGPKRLDEIMGVLKAYTTRVGAGP
                     FPTELRDEVGERFLNVGGEFGTTTGRPRRCGWLDLLVVEHAARLNGLTQFAVTKLDVM
                     NDLRRIKVAVAYEIDGRTVKRFPANPKMLERAVPVYEELDGWAGWRPEETMEIVRGGY
                     DALPREMRAYLEYIERCSKVPVRIVSIGKGREETVRR"
     gene            complement(8049..8690)
                     /locus_tag="PLJ11_02655"
     CDS             complement(8049..8690)
                     /locus_tag="PLJ11_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03600.1"
                     /translation="MRCNNCGLDYIADNAAYCPRCNAPLGQPGFQQPPQQYQQQAPDQ
                     QYQQPQQYQQPQQYPPQQQVGGQQPPQGYYPPQGYYQQPPVPPKRVPTVKRVDLTEIL
                     IALSGMFLLIGAFDNLSWLTVDGVPGGIIVLGIVSLLVGLLMLMDVLMPSLLKGLGEA
                     KDILVLVLALVFVIWGLVANFADTIDYGMSGSIIFVGGFSLLIAGMLRMGILK"
BASE COUNT         1668 a         2657 c         2954 g         1494 t
ORIGIN      
        1 caggatgcgg tggtgggtgg gacagtagcc ctgccagaga atcaatctgc gcccggtctg
       61 cctggcggcg tagtcgccga gactcatgtc cgggacgaaa atgatctccc ggtccgccgg
      121 gatgcggcgg acgatctcca cggcgttcgc ggaggtgcag cagacgtccg ccagggcctt
      181 gacggccgcg gaggtgttca cataggccac gacggccgcc tggggatgcc tttccttgaa
      241 cagcgccagc tccatggccg tgcacatggc ggacatgggg cacatggcct ccgattcggg
      301 aaggatgacc ttcttttcag gagagaggat cttggcgctc tcggccatga agtccacgcc
      361 gcagaagacg atcacctccg cgtcagtgcg ggcggcgatc tgcgccagtc cgagcgagtc
      421 ccccacatga tcggcgaggt cctggacctc gggcgattgg tagttgtggg ccaggaccac
      481 ggccttgcgc tgccccttga gctcctggat gcgcttcgcg acgttcattt cggcaagcca
      541 tgccaaaatc cgtataaata tttacttgac cagcctggag atcgtggaac gctggtcgtg
      601 caccgctgga aaggattatt cactgccaat ccctgtgcaa gggcgtgaag gcgcctgacc
      661 tcgattggct gttgtccggg gacccggtcc tctccgccct ggtgaaaagg gatctgatgg
      721 gcgatacgtc gcaaatgcca gccatcctga ccgatcgtcg gatcaaggag atggtgcggg
      781 atatcgcaga ttggccgtgg cctccctgcg tcagccataa gagtgccggg catcccatcc
      841 acaagttcgc cttcctggcc gacatcggac tggaactgga gggcaccaag gccggggatc
      901 tggcctggaa ggtccagaac ggaatggacg acggattgcc gaggataacc gtgaacattc
      961 ccgaacatta cggcggcacg ggaaggccga ccatgggctg gtcgctgtgt gacgcccccc
     1021 tgctgctcca ctcgctggcg caatgcttcc cgggaatgga tctggccccc ggcatgtccc
     1081 ggctgaacga tctggcaagg gagaacggaa tgccctgtgc ggtctccctg gagctcggca
     1141 ggttcagggg gccgggccgg aaggacgacc cctgccctat agcctgcttg tacgcactga
     1201 aggcggcgcg ggccatcgag ccggggaaga acgagaggct ggccaggacc atctccgcct
     1261 ctctgctcga tctttggtcc agcagccgcg aacggcatcc ttacatgttc ttcatgggca
     1321 ccgacttccg caagctcaag cttccgttcg tctggtacga cgtcctgagc gtgaccgatg
     1381 cgctgagcga gtgcccttgg gtgcttgacg atgcccgctt cagggacatg ctatcggtca
     1441 tcgggtccaa acaagaccct gagggccggt acacccccga atccgtatgg accgcctgga
     1501 agggttggga ctttacgcaa aagaaggaac cctcgcggtg ggtgacgttc gccgtcgaac
     1561 ggatccaccg ccgggttgga aggggttagt tcacttcagg atggcgtcca gcttcttgcc
     1621 gacggcatcc cagttcacat ggttccagaa ggcctcgatg tacttcggcc tcaggttctt
     1681 gtagtcgatg tagtacgcat gctcccagac gtccaggtcc atcaggacgc ggaagccggg
     1741 gatgacgttc acgttgtgct tctccacctg catgatcaac ggcttgtgca gcgccatgca
     1801 gtagacgagc accgcccagc cggagccctc ggcgctcgcc gcggcggcgc tgaactcctt
     1861 cttgaagcgg tcgatggagc cgaactcctt gacgagctcc tccttcagcc tccccgtcgg
     1921 ctccccgccc ttgcccgcgg ggcagagggt ctcccagaac accgaatgca ggtaatgccc
     1981 gccgacctgg aaggacaggt ccttcagcat ccccttcgca tccaggtctg ccccttcggc
     2041 ccgggccttg tccagcttgt ccaggatggc gttggcggcg ttgacgtagg cggcatggtg
     2101 cttggtgtgg tggacggtca actgctcctc gctgaggaac ggggcgaggt ccttgtaccc
     2161 ataggcgagc ttgggcagct catatcgctt cggttgctcc atgatgacac cttggcggcc
     2221 tatcggagct gatagcggat aatgattttg gttcccgatg gggaaggccg ataaggaaag
     2281 gggcgaatgg ggcgacggtc atcccatgga gtaccattcc ctgacggccc aggcatccga
     2341 ggcgtcgagg tccttgaggg cataggcctt gcccgcgggg gaggagagca gggagatgct
     2401 cagcgtgcgc aggcggccga gacgctgaaa gggattggcc aggagggaca gggactggac
     2461 gtgctcccgg ccgatcagca cctggtagag accgagggat cggaacccta tcgtaagctg
     2521 gtcgccgtcg atcgagaact tggcattgga gtgtcgagag cggccgagaa gcagggcggg
     2581 cacgaggagc aggagcgcgg cccctccgta cggtacgttc caatgatagg atgcgacgag
     2641 cagcaccgcg acgagcagcg ccataggcac ggtcgagcgg aagaggtagc ggcgccttgc
     2701 tctctccggc gcccttgtcg cccccgaagg gagcctgtac tccggaagga ggtccctcag
     2761 gaacccctcc acctcccgca tccgaagcat tgggtagagg acggacacct tctcctgctc
     2821 cttggcgccc ccgcccgcca cctccaccag gagggtgcag aggcctaggg gctgccgcag
     2881 cagtccctgc tggaccacca cggcctgcag gcggtgcagc cggaccgtga catggctccg
     2941 cttgatgacc ccccagtcga gctccaggcg gtcctcgaac ctgcggacgg tgaagttggc
     3001 gaactggagg gcgaagacca ccgtcgagag ggcccatgtg gcgaagaaca gggcgaggac
     3061 gatcccgagg ccgagcagga ggggcacgcc cgtgatgcgc gccaacgcct ggtcgatgag
     3121 ggtctgtggg atgtagtcga tcacctggga gagcgcgacc gcggccaccg agaacagcgc
     3181 catgaaccgg ccggacgtca ggccggcgag ccagaggtcc cggccctgca gggcgcgcag
     3241 ggccttggcc acctcctcca gcccgccctc ccgcgcctcc ccttccttgt tgaggtgctg
     3301 cctgatccgc tccgcctcct ccttggaaac ggcgcggagg ttgacctccg cgtccacgcc
     3361 caccccggcg gtcttgatct ggagcgttcg aaggcccagc gcctgctgca ccacgttcgc
     3421 gtagacgttg atggactgga ccctctcccg cctgatggtc cgctcgcgtt tgaacaggat
     3481 cccctgccgg atgtgcacca ttccctcctc gtaacgatag aagaagaagc gccagcgaag
     3541 gccgttatag acgatcgaga gggcgaagag ggcgaggagc gccacgatga aggcgccgac
     3601 gaagcccccg aaggtggaac tggggagcga taccgctatg actagggcga cgccgatgtt
     3661 cgagatgagg ccgcgccccg tgtcgagaag gacgagcagg ggatgctggc ggcggtactc
     3721 agacatcttc ctcactcacc ctggccaggg tggctatctc ccccctcagt gcctccgccc
     3781 tctccttggg cagcgcaggg atcgcgaacc gggtggcggc cgtggagacg tacagggtcg
     3841 tgaggccgaa gtaccgcatg agggggccgt actcggtgtc cacgaactgg acgcggacca
     3901 tcgggatgag gtgccgcttg atcaccaaga tgccgtgctg gatctccatc tccttctccc
     3961 ggacctcata cctccagaac cgcgtcctga gcaagggggc catgtagatc ccccagacgg
     4021 cgtagccgag ggcgagcgcc agggcgagcc accccgtcca tgcgggggcg tccagggccg
     4081 ggatcgcgaa ccagtaggcc agcgcgatcg ccatcaccac cgcggccccc atggccccgc
     4141 tgatggtcca taccgtacga gcccggggat cgatgcgctc cctcggttcg ttctccatcc
     4201 tcagcctttc tctgatgttc tccaacactt cttccgaccg gggggtcaag ctaccggccc
     4261 ccttaaggcc ctttcgccct caatgccctt ggcctcaagg aggaactatt attttccagg
     4321 ggaacattcc cttccgagga aggatgacga gcaggcgcga ggagctggcc gcgaagtact
     4381 tcaactgcac cgagcgagag agggcggtgt tcgaggcggg gataaagctg gggacgatct
     4441 accaccagtt cgtggggacc ccggtggccg cggcgaacgt ggagatcctg gagaaggcca
     4501 tcgaggacgg ggtgagggtg cagccgttcg tgaaggacgt gaaggtcggc atctcccgcg
     4561 aggccctgcg ccgcaagaag gacgagttcg actaccagac gctcaccggc cccatgatga
     4621 aggtggagct gaccgtgacg gtgggtggga ccaccgtggt ggcggccatg gactacaagg
     4681 acgatctgca ctatcctctg atgtacgtgc ggggaataga gtccaaataa atattataat
     4741 taatatttat aatgaggatt gcacgcccca acccagtgcg ccgttcctcg agaaaggcta
     4801 ttaagctagg atgacgataa gggtgtccta aagcatcgag ccccatcgat gcagggcacc
     4861 gaggaatttg atgaacaata tcaagatcgg catcactggg cttcccagcg caggtaagac
     4921 caataccctc atccaggtca tcaagatgct cgaggaggag gggtacacgg tcggagggat
     4981 gatcaccgag ccgatcttgg acgacaagaa gaggaacggc ctttatgtca tggactggat
     5041 gaccaaggag cggggcgtcc tcgcggcgac ggacatccac agcaagttca tggtcggcaa
     5101 gttcggcatc gacctggacg tgctccagga gataggggtc caggccctgg tcaacgcctg
     5161 cgagaaggcg gatatcatcg tcatcgacga ggtcgggaag atagaggtgg agagcgagcg
     5221 gttccgggac gccgtcaagg aggccctcaa gaccgagaag cccatgctcc tcaccctgca
     5281 taagaagtcc cgcaacccgt tgctccagga tatcaggaga cgcgacgacg tccggatcct
     5341 ggaggtcaca ccgatcaacc ggaacctcct gccctacaag ataatgaagc tgatgaaggg
     5401 cgagcttttg tgagcctgga aggaacgata ataaaggagg ggagcacgcg gctgctcgtc
     5461 ccccctctca tctcggagag ggggccgggg agggtcgagg gcaaggtgtt cttcaaccgc
     5521 cagatggcct tcaacaggga cgtggccgtc gtcatgttcc gttcggtcat ccagccccgg
     5581 aaatgcctgg atgccatggc cgccaccggt gcacgggggg tgcgcatctg caccgaggcg
     5641 ccgggggagt accggttcct gatcaacgac agggaaccgc tggcgggcga gtacatcagg
     5701 gagaacgtcc ggttgaactc catcgggaac gcggaggtcg gcaccgccga cctgcgctgc
     5761 ctgctgacca gggaggtctt cgactacatc gacctggacc cgttcggcac gccgatcccg
     5821 ttcctccccg ccgccctgca ggggatgacc cgcaagggcg tcctggcggt cacggccacc
     5881 gacaccgcct ccctctgcgg gacccacccc ggcaagtgcc ggaggcgcta catggccaac
     5941 agccagagga gcccgttcat gcatgagagc ggtctccgca tcctcatcgg gacgatcgtg
     6001 cgcatggccg cccaggagga ccgggggatc tccccgctcc tctgctacta cgcggaccac
     6061 tacttccggc tgttcctgag ggtgagggag ggggccgcgg ccgcggacgt cgcccttgct
     6121 cagatgggga tgatggccta cgaccgggcc acgggcgagc ggagggtggg ggagggaagc
     6181 atgggcccga tgtggctcgg gcctctgctg gaccacgggg ccctggccga ggccgcggcc
     6241 ccggaggggc tcgcctgccc ggaccgcgtg gaacggtacc tggagctgtg gagggaggag
     6301 ctggacgtgc ccttctacta cgagacggac gagctggcag caatgctcgg cctttcgccc
     6361 ccggggcgcg ccgagctgct gggggcgctc ggggacaggg ggcgcgtctc caggacgacg
     6421 ttctccccca ccggcttccg cacggaccgg ccgctggaag aggtgctcga ggcgttccgc
     6481 tccgcctgcc agtgagaaag ggtttatcta cgttggtccg atttgcgaag cggtgataga
     6541 gtgccgagca tagcggtcat cggagcccaa tggggggacg agggcaaggg taagatcact
     6601 gactacgagg ccgagcgcgc cgacatggtg gtgcgctacc agggaggctc caacgccggg
     6661 cacaccatca aggtgggcga cgaggtcttc gccctgcaca tccttccctc ggggatcgtc
     6721 cgcaagggtg tggtcaacgt catcgggaac ggggtcatcg tcgacctcat ctccctggac
     6781 cgggagatcg ccgaggtgga ggcccggggc cggtccgtgg aagggctcag gatatcggac
     6841 cgcgcgaaca tcatcctgaa ctggcaccgc cttctggacg gggccgagga acgtttccgt
     6901 ggggggagga ccgtgggcac cacggggaag ggaatagggc cgtgctactc ggacaagatc
     6961 gcccgctccg gcatccggat gggggacctg ctcgaccccg ccctcctcgc ggagcggctg
     7021 gacacggtca tgcccatgaa gctgaaggtc atggaggtcc tgaacgtccg tttggcgcag
     7081 acgagggacg agctgtactc ggagctcctg gcctacggga agcggtacgg caggtacatc
     7141 accgacacct ccgtgctcgt gaacgaggcc ctggacgagg ggaagaacgt cctcttcgag
     7201 ggggcgcagg gcaccatgct ggacattgat cacgggacgt atccctacgt cacctcgtcc
     7261 aattgcatcg cgggggccat atgcaccggg gcgggcgtcg ggccgaagcg gttggacgag
     7321 ataatgggcg tgctgaaggc gtacaccacc cgcgtaggcg cgggaccgtt cccgaccgag
     7381 ctgcgggacg aggtcggaga gcggttcctg aacgtcgggg gcgagttcgg gaccaccacc
     7441 ggccggcccc ggagatgcgg ctggcttgac ctcctggtcg tcgagcacgc cgcgcggctg
     7501 aacgggctca cccagttcgc cgtcacgaag ctggacgtga tgaacgatct tcgccgcatc
     7561 aaggtcgccg tggcgtacga gatcgacggc aggacggtaa agcgcttccc cgccaacccc
     7621 aagatgctgg agagggccgt gcccgtctac gaggaattgg acggatgggc cggctggagg
     7681 ccggaggaga ccatggagat cgtgcggggc gggtacgatg cgctcccgcg cgagatgcgc
     7741 gcgtacctgg agtacatcga gaggtgctcg aaggtgccgg tgaggatcgt cagcataggg
     7801 aaggggcggg aggagacggt ccggcgctag gccgcggccc cgtcgccctt tttcctcctt
     7861 ccatcgcggt tgccaagata ttccgctggg ggcgtcgccc gcgatcaccc ttcattgatt
     7921 ggaacgttga acggaacgag ggcgcctccc ctcgaccggc agggaccttc ccgtaagctc
     7981 aggggatgaa tagaacaggg ggaaagggaa tggaaagggg gggatgcccc cgttggtccc
     8041 ggtccgcatc acttcaggat gcccatgcgc agcatgccgg caatgagcag gctgaagccg
     8101 cccacgaaga tgatggaccc ggacatgccg taatcgatgg tgtcggcgaa gttcgccacc
     8161 agcccccaga tcacgaacac gagggccagc accaggacga ggatgtcctt ggcctcgcca
     8221 agccccttga gcagggaggg cattaggacg tccatgagca tgaggaggcc gacgagcagg
     8281 gagactatcc ctagaacgat gatccctcca ggcacgccgt cgaccgtgag ccacgacagg
     8341 ttgtcaaagg cgccgatcag caggaacatg cccgagagcg cgatcaggat ctccgtgagg
     8401 tccacgcgct tcaccgtcgg gaccctcttc ggcggcaccg ggggctgctg atagtaccct
     8461 tgcgggggat agtagccctg cgggggctgc tggccgccga cctgttgctg cggcggatac
     8521 tgttggggct gttgatactg ctgaggctgt tggtactgct ggtccggggc ctgctgctgg
     8581 tactgttgag gcggctgctg gaaccctggt tggcccaggg gagcgttgca ccttggacag
     8641 taggcggcat tgtccgcgat gtagtccaat ccgcaattgt tgcatctcaa gtgggattcc
     8701 ctccttagcg atactaaacg aattatatta ttttaataat tcgtactgaa tcattcatta
     8761 cggaattcag cca
//
LOCUS       DAOSYN010000035         8712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_6875_length_8712_cov_4.251242, whole genome shotgun sequence.
ACCESSION   DAOSYN010000035 DAOSYN010000000
VERSION     DAOSYN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6875_length_8712_cov_4.251242"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..731
                     /locus_tag="PLJ11_02660"
     CDS             111..731
                     /locus_tag="PLJ11_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03601.1"
                     /translation="MTADCRIEMAYIDPETYTSIVNHDLRKRILTKLYRSTRTSPISK
                     QELADSLGIDYHQLVYQLNHHLRDFWTVKEEQKVRGTRMELIEAAYPYAVFMTIGKDH
                     GIFSVDPLADLYGPVAKVGTRCDQCSREEAERCMAFAQSRFDSEPLTEAEQGVLAANG
                     RRAPYRPMDLALLAAIKGIPAGQRCVIDIPCQSCAFLRRTVRIEGL"
     gene            781..1884
                     /locus_tag="PLJ11_02665"
     CDS             781..1884
                     /locus_tag="PLJ11_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO03602.1"
                     /translation="MQSGGRTPTRFVVLGGYLGAGKTTLAAALGRELKAKHGRSVAII
                     TNDQGNVLVDTEYMRDAGFDVRDVLGGCFCANFGEFVKNARTLVSMGRPDIIIAEPIG
                     TSTAILGSVVAPLRSMYPDEFQVAPLFVVVDGTRAEETLEPAGGLGLGGGKLIPVHQV
                     REAEYVLLSKTDVLDEGQIRRAETLVHQEAPDAQVIPCSALDRRNLDRIVEIILSDQV
                     STKEPKEVDQRLFAVEKASMGWFNAYAKVAAEGRLDINAFLTALIRGVATSFPPEAIG
                     HVKAMLTSPTASAKMSLVGDRMQVDGLKGGRYLTGEGKLVLNARVTASPDELKGALER
                     ALHEAARETGAQIAEMGEACFTPRPETPSMFKK"
     gene            2005..2448
                     /locus_tag="PLJ11_02670"
     CDS             2005..2448
                     /locus_tag="PLJ11_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOO03603.1"
                     /translation="MPVVDAMNRRILQLLVNDCRMTHNEIAAKLKRSPSTVRDRIRRM
                     EDDGIILGYFAVVNNERMGIKADAVLFANLRPGVMAEELRKLKGIEEVKEVLQITGPR
                     RVMVRLQARDPAGLEQALTTKVLPVGLEDPELRMVTSSANRLPGS"
     gene            complement(2445..3278)
                     /locus_tag="PLJ11_02675"
     CDS             complement(2445..3278)
                     /locus_tag="PLJ11_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03604.1"
                     /translation="MRDACKPKGHSHECDCGRECMAIADGRAVIDLDLSSERPMHYTT
                     LNCAVRKAMRQGCGSIEIRGVIGQRYLAATASVPGLHIAVHGTPGNDLGAFLNGPTIE
                     VFGNAQDMTGNTMNAGRIIVHGNAWDVTGLAARGGQILVKGDTGYRVGIHMKEFGDVR
                     PTLVIGGTAKDHLGEYMAGGTALVLGMGEEGVPVGRNLGAGMHGGRIFVRGEVPPDRL
                     GPGAALAPVLPSDTREIMALLDDFERAFSVKPPRSMDGYVKVVPSSSRPFSGYYDKTS
                     V"
     gene            complement(3278..4813)
                     /locus_tag="PLJ11_02680"
     CDS             complement(3278..4813)
                     /locus_tag="PLJ11_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOO03605.1"
                     /translation="MSEGAGAGKRLDHRLPERFVPTIDHDLCRACQRCVKDCSYQALE
                     FRGSRVVPAGGCVACGRCIAVCPANCIEVRPVPPQFAPHGNWSERARRAILAQASSGG
                     VLLSSCGADDDRPAIFDDLLLDAAQVTNPSIDPLREPVETVTYLGRRPGRLEVRDGRV
                     VVREGDTPLIRSDMPLMFGHISLGSISYNAQKALFMAAKELNIVAGSGEGGLHPDLYE
                     YADRICSEVASGRFGIKPEYLKEVAAVEIKIGQGAKPGHGGHLPGEKVTAMISETRMI
                     PLGTDALSPYPHHDVYSIEDLAQLISVLKEATEYRTPVGVKIAAVHNVGAIASGVVRA
                     GADFLTVDGFKGGTGAAPRVIRDHAGLPIEVAISAVDQRLVREGLRNRVTVCAGGGVR
                     SSADMIKALALGADVVMVSTAALIAMGCRVCQQCHRGLCSWGIATQRDDLVARLDPYI
                     ASARVVHLFHAWNEELKEVLGAMGIDSVESLVGNRDRLRYRGPNPKIAEVMGVKHIGE
                     GWG"
     gene            complement(4810..6030)
                     /locus_tag="PLJ11_02685"
     CDS             complement(4810..6030)
                     /locus_tag="PLJ11_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03606.1"
                     /translation="MHMEGRERAERGRTPMTGESYVATTPPGWQGQLGGCGIASCISL
                     DGRTIPGDSIARMLTIMEERENGLGAGYACYGLFPERKDQYCLQLFFDDEEGKRSAEE
                     FLAGRLDIVHDEKVFIRRNSTVTPPYPIVWRFFANVPERREWRGALDLSEEDYMVELV
                     QHINGNVPKAFCVSSGKDMAVFKGNGYSYEIADLYDIGRYSGTMWLSHSRFPTNSPAW
                     WAGAHPLSLLDWGVCHNGEITSYGVNRKLVEMNGYRCTVLTDTEVVVYLWDLLVRRHR
                     LPVEVAAFAMAPSPYAEIALLPPAERRLAEWVRIAYREAFLNGPFSIIVGRSQPEISL
                     IALADRKKLRPMIAGVSPDGGLAYCASEESAIRIVEPEARTWAPGAGNPFIAAVGKGV
                     LRKGTEPSFQGVRL"
     gene            6456..7112
                     /locus_tag="PLJ11_02690"
     CDS             6456..7112
                     /locus_tag="PLJ11_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO03607.1"
                     /translation="MTEGAVDMTSSRTALVPQLPDLDKIRQMRKRLNLSQRELASLAG
                     VSQSLIAKIERGTIDPSYSNVRKILNAFEEVIRRRKVEGVRSGTQLTVGDLATRGVVF
                     VTPDQSVGEAVERMMRGRFTQLPVIVGERIVGGLTDDAIRDYTIEQTKARKKTYEEVM
                     RTRIEEIMDAPFPILSEDTPIDLASFHLQREEAILVSRKGAIVGILTSADFLNLGLNQ
                     "
     gene            7147..7758
                     /locus_tag="PLJ11_02695"
     CDS             7147..7758
                     /locus_tag="PLJ11_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HOO03608.1"
                     /translation="MKKRELEIILQALPSFPSPRPELEQYATPAVIAADMLFEALARG
                     DVQGRRVLDLGCGAGPLGIGAALLGAEHVTGLDSDPSALAQALANAARAGVEVEYIQS
                     DVLDARGAWDTVIMNPPFGAQRRGADRPFLSTAMRCAPVIYSLHLSQTEAFVERFARR
                     SGYTAEPLKRYKFGIPYMFEFHSKAKKEVDVTLFRILKAGVLE"
     gene            7755..8336
                     /locus_tag="PLJ11_02700"
     CDS             7755..8336
                     /locus_tag="PLJ11_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Csl4"
                     /protein_id="HOO03609.1"
                     /translation="MSDRRKVLPGDEVAVAEEYLPAEGTYEEGGIVYSALAGELKVND
                     SDKTVSVEADNPMVSLSIGDSVFCEITDVRPSMAICEVVAVEGRPRNITGDTNGTIHV
                     SKISQDYVQDVGREYRPSDIIRAKVIQVKPSIQLNTAHPHFGVVKALCRTCRSHLRRV
                     EKGLYCDHCEAFESRKIADDYGFVETILLTGKE"
     gene            8529..>8712
                     /locus_tag="PLJ11_02705"
     CDS             8529..>8712
                     /locus_tag="PLJ11_02705"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOO03610.1"
                     /translation="MRLVALLSGGIDSPVAAFLMAQRGAEVALLHMDNRPFCGDLQLR
                     KAGLLAGALREATGQKM"
BASE COUNT         1644 a         2833 c         2707 g         1528 t
ORIGIN      
        1 acctttccgc ccaccatgtc tctgcatgcc gtcgagggac cttctccgat agcgatacag
       61 gtagtctgcg gcgtcaacgg ttaaatattt gagaacggaa tcaagaggcg ttgacagccg
      121 attgccgcat tgagatggcg tacatcgacc cggagacgta tacgtcgatc gtcaaccacg
      181 atctgcgcaa gcgcatcctc accaagctct accgctcaac aaggacctcg cccatcagca
      241 agcaggagct ggccgattcc ctcggcatcg actatcacca gctcgtctac cagctcaacc
      301 accatctacg ggatttctgg accgtgaagg aggagcagaa ggtacggggg acccgcatgg
      361 agctcataga ggccgcctat ccgtatgcgg tcttcatgac gatcgggaag gaccacggca
      421 tcttctcggt ggacccgctg gccgatctct acgggccggt ggccaaggtc gggacccggt
      481 gcgaccagtg cagcagggag gaggcggaga ggtgcatggc cttcgcccag tcccggttcg
      541 actccgagcc gctcaccgag gcggagcagg gcgtgctggc ggccaacggc cggagggcgc
      601 cctaccgccc catggacctg gcgctcctgg cggccatcaa ggggatcccg gccgggcaga
      661 ggtgcgtcat cgacatcccc tgtcagagct gcgcctttct gcggcggacg gtgcgcatcg
      721 agggactgta atattggagc cccaggaaat gtataagccc ggaggggcga tacccggggc
      781 atgcagtccg gagggaggac gcccacccgg ttcgtggtgc tgggcggcta cctgggcgcg
      841 ggcaagacga cgctggccgc cgcgctcggc agggagctga aggccaagca tgggcggtcc
      901 gtggcgataa tcaccaacga ccagggcaac gtgctggtgg acacggagta catgagggac
      961 gccggcttcg acgtcaggga cgtgctgggc ggctgcttct gcgccaactt cggggagttc
     1021 gtcaagaacg cccgtaccct ggtctccatg ggccggccgg acataatcat cgccgagccc
     1081 ataggcacct cgaccgccat cctcggctcg gttgtcgccc ccctgcgcag catgtacccc
     1141 gacgagttcc aggtcgcccc cttattcgtc gtggtggacg gcacgagggc cgaggagacc
     1201 ctcgaaccag cggggggcct cgggctcggc ggggggaagc tcatccccgt ccaccaggtg
     1261 cgggaggcgg agtacgtcct gctgtccaag accgacgtcc tggacgaggg gcagatcaga
     1321 agggcggaga ccttggtgca ccaggaggcc cctgatgctc aggtgatccc ctgctccgcc
     1381 ctcgaccgca ggaacctgga caggatcgtg gagatcatcc tctccgacca ggtgagcacc
     1441 aaggagccca aggaggtgga ccagaggctg ttcgcggtgg agaaggcttc catgggctgg
     1501 ttcaacgcct atgccaaggt cgctgccgaa ggccgactgg acatcaacgc cttcctgacc
     1561 gcgctcatcc gtggcgtggc aacatccttc cctccggagg ccatagggca cgtgaaggcc
     1621 atgctcacct cccccaccgc ctcggccaag atgagcctgg tgggggaccg catgcaggtg
     1681 gacggcctga agggcggccg ctacctcacc ggcgagggca agctcgtcct caacgcccgg
     1741 gtcacagcct cgcctgacga actcaagggg gcattggaga gggcgctcca cgaggcggcg
     1801 agggagaccg gcgcgcagat cgcggagatg ggggaggcgt gcttcacccc caggcctgag
     1861 accccgtcca tgttcaagaa atgagcccgg ttccgtccgt tccctcaatc ttttcaataa
     1921 ttcccagctt tatttcattt ttcccttcaa ttgccgtgct tccatcggaa tcattatggg
     1981 aaccggggcc ctttgaaggg cgacatgccc gtggtcgatg cgatgaaccg gcgcatcctg
     2041 cagctcctcg tcaacgactg caggatgacc cataacgaga tagccgccaa gctgaaacgc
     2101 tccccctcca cggtgaggga ccgcatccgg cgcatggagg acgacgggat catcctcggc
     2161 tacttcgccg tggtcaacaa cgagcgcatg ggcatcaagg cggacgcggt cctctttgcc
     2221 aacctcagac caggcgtcat ggcggaggaa ctccgcaagc tcaagggcat cgaggaggtc
     2281 aaggaagtgc tgcagatcac cggcccccgc cgggtgatgg tccgcctgca ggccagggac
     2341 ccggcgggcc tggagcaggc gctgacaacg aaggtcctgc cggtcgggct ggaggacccc
     2401 gagctgcgga tggtcaccag ctctgccaac cggctaccag gttcctaaac gctcgtcttg
     2461 tcgtagtatc cggagaaggg cctgctggag ctggggacga ccttgacgta cccgtccatg
     2521 ctcctagggg gcttgacgga gaaggccctc tcgaaatcgt ccaacagcgc cattatctcc
     2581 ctcgtgtcgc tggggagcac cggggcaagc gctgcgcccg ggccgagcct gtccgggggc
     2641 acctcgcctc gaacgaagat acggcctcca tgcatccctg cccccaggtt gcggcccacc
     2701 ggcacccctt cctcacccat gcccaggacc agtgcggtcc cgccggccat gtactcgccc
     2761 agatggtcct tggccgtgcc accgatgacc agcgtcggtc tcacatcccc gaactccttc
     2821 atatggatcc ccacccggta gccggtatcg cccttgacca ggatctgccc cccgcgggcg
     2881 gctagcccag tcacatccca ggcgttgccg tggacgatga tgcgacctgc gttcatcgtg
     2941 ttcccggtca tgtcctgcgc attcccgaac acctcgatgg tcgggccgtt caggaacgcc
     3001 cccaggtcat tgccgggggt cccgtgcacc gctatgtgca ggcccggcac agaggcggtc
     3061 gctgcaaggt acctctggcc aatgacccct cggatctcga tggagccgca cccttggcgc
     3121 atcgccttgc gcacggcgca gttgagggtg gtatagtgca tcggccgttc cgaggaaagg
     3181 tccaggtcaa tgaccgccct gccgtcagca atggccatgc actccctccc gcagtcgcac
     3241 tcatgggaat gccccttcgg cttgcatgcg tcccgcatca gccccacccc tcccctatat
     3301 gcttgacccc catcacctcc gcgatcttgg ggttcgggcc gcggtaccgc aagcggtccc
     3361 tgttgcccac gaggctctcc acggaatcga tgcccatagc cccgagcacc tctttgagct
     3421 cctcgttcca ggcatggaac aggtggacga cgcgcgccga ggcgatgtag ggatcgaggc
     3481 gggcgacgag gtcatccctc tgcgtggcga tgccccagga acataggccg cgatggcatt
     3541 gctgacatac ccggcatccc attgcgatca aggctgcggt ggacaccatg accacatcgg
     3601 cgcccaatgc aagcgccttg atcatgtccg ccgaggagcg taccccgccc ccggcgcaga
     3661 ccgtgaccct gttgcggagc ccctcccgca caagccgttg gtccaccgcc gagatggcga
     3721 cctcgatggg cagccccgca tggtcccgga tcaccctggg ggcggccccg gtgcccccct
     3781 tgaaaccatc caccgtcagg aagtccgccc ccgcccgcac cacgccggag gcgatcgcgc
     3841 ccacgttgtg cacggctgcg atcttcaccc ccaccggcgt ccggtactcg gtggcctcct
     3901 tgagcaccga gatcagctgg gcgaggtcct ctatgctgta cacgtcatga tggggatagg
     3961 gggacagcgc gtccgtccct aaggggatca tcctggtctc ggagatcatc gcggtgacct
     4021 tctccccggg caggtgcccc ccgtgcccgg gcttcgcccc ctggccgatc ttgatctcca
     4081 ccgctgccac ctccttgagg tactccggct tgatgccgaa gcggccggag gcgacctccg
     4141 aacagatccg gtccgcgtac tcgtagagat ctggatgaag cccgccctcc ccgctgccgg
     4201 ccacgatgtt cagctccttg gcggccatga acagggcctt ctgggcgttg tagctgatgg
     4261 accccaggga gatatgcccg aacatcaggg gcatgtccga tcggatgagg ggcgtgtcgc
     4321 cctcccggac gaccacccgc ccatcccgca cctccagcct tcccgggcgc cttcccaggt
     4381 aggtgacggt ctccaccggc tcccgcagcg ggtcgatgga agggttggtg acctgggcag
     4441 cgtccagcag caggtcgtcg aatatggccg gacggtcgtc gtccgccccg caggacgaga
     4501 gcagcacccc gccggagctg gcctgggcca ggatcgcccg gcgggccctc tccgaccagt
     4561 tcccgtgagg ggcgaactgc ggggggaccg ggcggacctc tatgcaattg gccgggcata
     4621 cggcgatgca tcggccacag gccacgcatc ctccggcagg gacgaccctc gagcccctga
     4681 actcaagggc ctgatatgag cagtccttca cgcatcgctg acaggcccgg cataggtcat
     4741 ggtctatggt gggaacgaac ctttcgggaa ggcgatggtc caatcgttta ccggcgcccg
     4801 caccctcgct cacagcctca ccccctgaaa ggaaggctcg gttcccttgc gtaggacccc
     4861 cttgccgacc gcggcgatga aggggttccc tgcccctggc gcccaggtgc gggcctccgg
     4921 ctccacgatc cggatggcgc tctcctcgct ggcacagtag gcgagaccgc cgtcggggga
     4981 cacgccggcg atcatcggcc tgagcttctt gcggtcggca agggcgatga gggatatctc
     5041 cggctggctg cggccgacga tgatgctgaa cggcccgttc aggaacgcct cccggtacgc
     5101 gatgcgcacc cattcggcca agcggcgctc ggccggcgga aggagggcga tctctgcata
     5161 gggggagggg gccattgcga aggccgccac ctccaccggc agccggtgcc tgcgcacaag
     5221 aaggtcccac aggtagacga cgacctcggt gtccgtgagc accgtgcacc tgtatccgtt
     5281 catctccacc aatttgcggt tgaccccgta ggaggtgatc tcgccgttgt gacacacccc
     5341 ccagtccagg agcgagaggg gatgcgcccc cgcccaccag gccggggagt tggtggggaa
     5401 gcgggaatgg gacagccaca tcgtgcccga gtaccttcct atgtcgtaga ggtcggcgat
     5461 ctcgtaggaa taaccgttcc ccttgaagac cgccatgtcc ttgccgctgg agacgcagaa
     5521 tgccttgggc acgttcccgt tgatatgctg gaccagttcg accatgtagt cctcctcgct
     5581 caggtccagg gctcctctcc attccctcct ctcaggcaca ttggcgaaaa accgccacac
     5641 gatggggtag ggaggcgtga cggtggagtt ccgtctaatg aagaccttct cgtcatggac
     5701 gatgtccaat cggccggcca ggaactcctc cgcgctcctc ttcccctcct cgtcatcgaa
     5761 gaagagctgc aggcagtact ggtccttcct ttcggggaat aggccgtagc aggcgtaccc
     5821 ggcgcccagc ccgttctccc tctcctccat gatggtgagc atgcgggcga tgctgtcccc
     5881 cgggatggtc ctcccatcga gggagatgca tgaggcgatc ccacagccac ccagctgccc
     5941 ctgccatccc ggaggcgttg tggcaacata gctctcgccg gtcatcggcg ttctcccccg
     6001 ttcagccctt tcccgtccct ccatgtgcat catcccttcc cagccccatc cgtgcgacca
     6061 tgggcccgcc cggagcgatc atccggggtc ggccataata ttttactttt ttccatagac
     6121 aaacatctaa tatatccaat cattaacgct caattttcta tctgtttttc atctattaga
     6181 tgaatgacca tttacaaaag gggcaaaaag gttgtgatat aaattccttc cgttgttaac
     6241 ggtttattat cgtaaaacat ttcacgaata tcgtagaaaa ttatcaaaat cagaacgatt
     6301 atcgggccag atatatgccc aatgcagaaa aacagctcca ttgctcgatc gaatggtcaa
     6361 ttcctaatac cgtcccgatg ccgtcccaat gattcctatt aaattcaata tacagatctg
     6421 gaaaatgata tctatcaggt aatgactatt gaaatatgac cgaaggtgca gtcgacatga
     6481 cctcctcccg cacggccttg gttccgcaat tgcccgacct ggacaagatc cgccagatga
     6541 gaaagcgcct caacctttcc cagagggagc tggccagctt ggcgggggta agccaatcgc
     6601 tcatagccaa gatagagagg gggaccatcg acccctccta cagcaacgtt cggaagatcc
     6661 tcaacgcctt cgaggaggtc atccgacgcc gcaaggtcga gggcgtgcgg tccgggaccc
     6721 agctcaccgt cggagacctg gccacccgtg gggtggtctt cgtcaccccc gaccaatcgg
     6781 tgggcgaggc cgtggagagg atgatgaggg gccggttcac ccaattgcct gtgatcgtcg
     6841 gggagcgcat cgtcggcggg ctcacggacg acgccatccg ggactacacc atcgagcaga
     6901 ccaaggcccg caagaagacc tatgaggagg tcatgcgcac ccggatcgag gagataatgg
     6961 acgccccctt cccgatattg agcgaggaca ccccgatcga cctggcgtcc ttccatctgc
     7021 aaagggagga ggccatactg gtctccagga agggggccat agtcggcatc ctcaccagtg
     7081 cggacttcct gaacctgggg ctgaaccagt gaggcgctag ggggcattcc gtcaggacgg
     7141 tggatcatga agaagaggga gctggagatc atcctgcagg ccctgccctc cttcccctcc
     7201 cccaggcccg agctggagca gtacgctacc cccgccgtca tcgccgcgga catgctgttc
     7261 gaggccctgg ccagggggga tgtgcaggga aggagggtcc tggacctggg ctgcggcgcg
     7321 gggcccctgg ggatcggggc cgcgctcttg ggggcggagc acgtcaccgg tctggacagc
     7381 gacccctcgg ccctcgcgca ggcgctggca aacgccgcgc gggcgggggt ggaggtcgag
     7441 tacatccaat cggacgttct ggacgcccgg ggggcatggg acacggtgat aatgaacccg
     7501 cccttcgggg cccagcgaag gggcgccgac aggcccttcc tgagcacggc catgagatgc
     7561 gcccccgtca tctattccct ccatctgagc cagacggagg cgttcgtcga gcggttcgcc
     7621 aggaggtctg ggtacaccgc cgagccgtta aaaaggtata aattcgggat cccgtatatg
     7681 ttcgagttcc atagcaaggc gaagaaggaa gtcgacgtca cgctatttcg cattctcaag
     7741 gcaggtgtgt tggaatgagc gacaggagaa aggtattacc aggtgacgag gtggccgtgg
     7801 cggaggagta cctcccggcc gaaggcacct atgaggaggg cggcatagtt tactccgccc
     7861 tcgccggcga gctcaaggta aacgattcgg acaagaccgt gagcgtggag gcggacaacc
     7921 cgatggtctc cctgtccatc ggcgactccg tgttctgcga gatcacggac gtccgcccct
     7981 ccatggccat ctgcgaggtc gtggcggtcg aggggcggcc ccgcaacatc acgggcgaca
     8041 ccaacgggac catccacgtg tccaagatct cccaggatta cgtgcaggac gtcggaaggg
     8101 agtacaggcc aagcgatatc atccgggcca aggtcatcca ggtgaagccc tccatccagc
     8161 tcaacaccgc acatccccat ttcggggtgg tcaaggccct ttgccggacg tgccgttccc
     8221 atctccgcag ggtggagaag gggctctatt gcgaccattg cgaggcgttc gaatcgcgca
     8281 agatcgccga tgactacggc ttcgtggaga cgatcctgct caccggcaag gaataaagga
     8341 ggggatcgcc ctgtccgaga ccgacctggc gcgggagatc aaggacctcc gcgaggaggt
     8401 caggcagatg aaggagctca tcgacctgct gatctccctg gtcatcgaga acgacgatcc
     8461 ggaggaggat gatctggata gcatccttcc cggtggtgcg gacatcccgc gcctcaataa
     8521 ttgagagcat gaggctcgtg gctcttctct cggggggcat cgattccccc gtggcggcct
     8581 tcctcatggc gcagaggggg gcggaggtcg ccctcctcca catggacaat cgacccttct
     8641 gcggggacct tcagttgagg aaggcggggc tcctcgccgg ggcgctccgc gaggcgaccg
     8701 gccagaagat gc
//
LOCUS       DAOSYN010000036         8664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_6934_length_8664_cov_4.180741, whole genome shotgun sequence.
ACCESSION   DAOSYN010000036 DAOSYN010000000
VERSION     DAOSYN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6934_length_8664_cov_4.180741"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLJ11_02710"
     CDS             complement(<1..1025)
                     /locus_tag="PLJ11_02710"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOO03611.1"
                     /translation="MEDVVIIGAARTAIGKFNGSLRDVPAPVLGSVAIRAAVERSGLM
                     PSDIEECLMGNVLSASLGQNPARQAAVGAGLPVEIGSTTINKVCGSGMKAVMLAANAI
                     KAGEYEVIVAGGMENMNAAPYLLPQARFGYRLGDGKAIDHMVRDGLWDAFTDVHMGMT
                     AETVAERFHVTREQADLLAYESHVKAHRAQSEGRFDREIAPVMVKGKKGETVFDKDEG
                     IRPDTTPEVLAKLSPAFKKDGIVTAGNASQLSDGAAALVVCSRRYAEARGIGPMAEIV
                     GYATGGTRPEWIMEAPIPTTRRLLDRLGMDIGDIDLFEHNEAFATASVAVRSELGVPE
                     DRFNVNGG"
     gene            complement(1118..2410)
                     /locus_tag="PLJ11_02715"
     CDS             complement(1118..2410)
                     /locus_tag="PLJ11_02715"
                     /inference="COORDINATES: protein motif:HMM:NF020078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HOO03612.1"
                     /translation="MFVALDDTDSLESMCTTYLATELIKALGVYDLIGLPRLVRLNPA
                     VPWKTRGNGALCLRLGIGRGEPTMVGELDGMPVYSYGRTAEEADPEFVLTVAEDVIGR
                     LARTEEGASPGLVISAEKPAQRFYWQAVRGIVEKTDVLQELQRLGADLVGWEGGRGLI
                     GATAAMAWRPRDRTYEIITYRARERWGTPREIDPLSVRAMDLERPSTFNSFDHGTGRM
                     AIAPHTPCPVLFGVRGDDPRELMAAAFSIRSEPPARWLMFLSNQGTDDHLIKGAPGSP
                     DRSYVFLGTVATVPRTIAGGHVLFQVTTSQGPLECAAYEPTKGFRDVVRGLRPGDRLR
                     LFGELRAEPRTLNLEKLQVIATAPERRKVANPRCPVCGGATKSMGAAGGHRCKRCGAR
                     TSDPPVTAEVPRTVAPGWYEPPVCARRHLSKPLKRMGL"
     gene            2510..2857
                     /locus_tag="PLJ11_02720"
     CDS             2510..2857
                     /locus_tag="PLJ11_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03613.1"
                     /translation="MSKPLTVEWGLEIAVSLIVLGAVLLIVGIMDVLHLYLDLGGWGF
                     YSGAVGVLLLIVGIVWMVSILQRMRKFKLLFQEKSKAVFVRSLDELEYTVWRLPSKYD
                     ALLLEKRREMGLK"
     gene            complement(2988..4211)
                     /gene="hflX"
                     /locus_tag="PLJ11_02725"
     CDS             complement(2988..4211)
                     /gene="hflX"
                     /locus_tag="PLJ11_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOO03614.1"
                     /translation="MEELCRSADIELLYEVVQRRPLPDRAYFIGRGRYGALKEVLRER
                     PVDVLVINGDLRPSQHFNLESGLNVECLDRIAVVLDIFTSRAEGREAKLQVELARLRY
                     QVPLMREWVHSAKAGEHPGFLGGGEYAVDVYYNLIRRRIARIEAGLQELARDGDMRRG
                     VRRYKGFQAVCLAGYTNAGKSSLMERMTGEEVLIADRMFSTLDTTTRRMGPSKVLLTD
                     TIGFLKDLPPFLVESFRNTLQDVFTADLVVLVVDSSEPIPIIKDKVMAVRDVLGRGLM
                     AGELIVALNKIDQGRDNLAEAMMVVRDALEPMCTLGVSALTGEGIDALIAIIDSYFRP
                     PITVNVAMRNDAGAASEVSWLYDSFFVESVEYGDDVKVAFRCASDEVDRVTERLRSLP
                     GTKWVSTATDAVDDN"
     gene            4482..5720
                     /locus_tag="PLJ11_02730"
     CDS             4482..5720
                     /locus_tag="PLJ11_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HOO03615.1"
                     /translation="MESNIFTQHLERKQIFKKNNREILRPSYIPDVLPHRQEEIDSLA
                     SILVTALRGERPSNILIFGKTGTGKTACVKFIGNEIKKAGGEAEPVTFIYMNCEVVDT
                     PYGVLQNIGNQFVKDFDQRIPFTGLSIMQVYTMLRDKLDEQNQVIVIALDEIDKLVYK
                     SGDDVLYHLSKINDDLKNARVSIIGISNDLLFTEMLDPRVRSRMEGEKMVFPPYNADQ
                     LKDILRQRAALAFDDGVLEEAVIPLCAALAAQEHGDARRALDLLRIAGEIAERDNAER
                     VAELHVLKAKNKMELDTVSEAIRTLPTQSKMVLMCIVMNVERGAGRLTTGDVYETYKE
                     ISRVLGMGPLTQRRITDLLSELDMMGIIHARVRSFGRGGRTKEIDLGVPLADTKKVLE
                     EDDTFAGLKHFKQKNQMTLI"
     gene            complement(5721..6608)
                     /locus_tag="PLJ11_02735"
     CDS             complement(5721..6608)
                     /locus_tag="PLJ11_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOO03616.1"
                     /translation="MSELSGKIKGVIVRYKVLVGGLLTVAILLLAMLVYAGMWPPVYV
                     VESASMQHSDTESALGTIDTGDLVLVRSSDDVSVRTYVDCFLEGDRSFGDFGDVIIYE
                     RYGREEYTPVIHRAMLRLEFNESSGSFDLPSLARLPSAKWGNGGLEEGRWWNLTGSVE
                     IYDVGYRSALLHVDLFPLLKYAHDGLITMGDHNVVRSDGQWLGVYDQSAATICLEPVM
                     DGWVIGVAKAEIPWIGLINLWVNGNMPANTPENSKAGLVAVIVLVIAVPLALDLTGMF
                     LERRGIDPWAWLRGRLRRK"
     gene            6685..7140
                     /locus_tag="PLJ11_02740"
     CDS             6685..7140
                     /locus_tag="PLJ11_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOO03617.1"
                     /translation="MSERPDNDTYFMRMAVLVATRSTCLRRQVGAVIVKEKRVLTTGY
                     NGAPRGLRHCEEVGCVRKENNIESGTRHELCRGVHAEQNAVIQGAYFGVSIKEGTVYT
                     TNFPCVLCAKILLNAGIKEIVYLNAYVDELSKQILDEAGIKVRRYDRPL"
     gene            7372..7716
                     /locus_tag="PLJ11_02745"
     CDS             7372..7716
                     /locus_tag="PLJ11_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03618.1"
                     /translation="MNRSEILLQIKDAEAKAQQIVKDAEERQRATIAAARKEAATRMK
                     EADEALKAEYEGRLAKERASIASERQALLNKGREEAEAIRKRAEANVPKVITHLLQSF
                     ERTLNVAANPND"
     gene            7709..>8664
                     /locus_tag="PLJ11_02750"
     CDS             7709..>8664
                     /locus_tag="PLJ11_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03619.1"
                     /translation="MTRVLIVGSRGALKDTIELLYRLEAAQLIDFSAEEEGFSLGAPL
                     PESSEASQRLLKLRSLEKDMGVDAAEVRGRMDVAQIEKDLAATIAELEVEITSVTESR
                     NKLAGRINELEAERAVLKGFLGIPLDMEMYSGYESLRVLTGKVRGDPSVAVRAAAPQS
                     EVFLSDSGDFVAVFVAKAQADEAQRALAQFGFTEVPVPAKSGSPQQLVARADEELTAL
                     RASLEEAVRKLEELRERHAAFVKAADEHLSIVVEKAETPLRVGTTEYAFAMDAWVPTS
                     DVPKVEKAIAEGLGGKVHLEALATAPRKEAHASHEGEENPKSL"
BASE COUNT         1677 a         2751 c         2646 g         1590 t
ORIGIN      
        1 ccgccgttga cattgaagcg gtcctcgggg acccccagct cggagcgcac ggccacggag
       61 gcggtggcga aggcctcgtt gtgctcgaag aggtcgatgt ctccgatgtc catccccagc
      121 ctgtcgagga gcctgcgggt cgtggggatg ggcgcctcca tgatccattc cgggcgggtg
      181 ccgcccgtcg cgtacccgac gatctccgcc atcggcccta tccccctggc ctcggcgtac
      241 ctgcgcgagc agaccacgag ggcggccgcc ccgtcgctga gctgggaggc gttgccggcg
      301 gtgacgatgc cgtccttctt gaaggccggg gacagtttgg ccaacacctc gggggtggtg
      361 tccgggcgga tgccctcgtc cttgtcgaag accgtctcgc ccttcttccc cttcaccatc
      421 accggggcga tctccctgtc gaagcgcccc tcgctctgcg cccggtgcgc tttgacgtgg
      481 gattcatacg ccagcaggtc ggcctgttcg cgggtgacgt ggaagcgttc ggccaccgtc
      541 tccgccgtca tgcccatgtg cacatccgtg aacgcatccc agagcccgtc gcgcaccatg
      601 tggtcgatgg ccttgccgtc cccgagccgg tagccgaagc gggcctgcgg gagcaggtag
      661 ggcgccgcgt tcatgttctc catccccccg gcgacgatga cctcatactc cccggccttt
      721 atggcgttgg ccgcgagcat caccgccttc atccccgagc cgcagacctt gttgatcgtg
      781 gtggagccga tctcgacggg cagccccgcg ccgaccgccg cctgcctcgc cgggttctgc
      841 cccagcgagg cggagagcac gttgcccatg aggcattcct ctatgtccga gggcatgagc
      901 ccagagcgct ccacggccgc cctgatggcg acgcttccca gtacgggcgc tggaacgtcc
      961 ctgaggcttc cgttgaactt gcctatggcg gtcctggccg cgcctatgat cactacgtcc
     1021 tccatggtcc tcaccccgcc agctggcttg tctggctcga gagtcaaacg gcgagggggt
     1081 ataaatcagt gtcgatatcg gcctacggct gatgtcgtta cagccccatg cgcttcagcg
     1141 gcttgctgag gtggcgccgg gcgcacacgg gcggctcgta ccatccaggg gcgacggtcc
     1201 tgggcacctc tgcggtgacc gggggatcgc tcgtcctcgc cccacaccgc ttgcagcgat
     1261 ggccccccgc cgcgcccatg gatttggtag cccctccgca cacggggcag cgcgggttgg
     1321 ccaccttcct tcgctccggg gccgtggcga tcacctggag cttctccagg ttcagcgtcc
     1381 gcggctccgc cctaagctcc ccgaagagcc ggagacggtc cccggggcgg aggcccctga
     1441 ccacgtcccg gaaccctttg gtcggctcgt aggccgcgca ttcgagcggt ccctgggacg
     1501 tcgtcacctg gaacagcaca tggccgccgg caatggtgcg ggggacggtg gcgaccgtcc
     1561 ccagaaaaac gtaggaacgg tcgggagagc ccggcgcacc tttgatgagg tggtcgtccg
     1621 tcccctggtt gctcaggaac atgagccatc gcgcgggcgg ctcggaccgg atgctgaacg
     1681 cggcggccat gagctcccgg ggatcgtccc cccgcacccc gaatagcacc gggcaggggg
     1741 tgtgcggggc tatggccatc ctcccggtgc cgtggtcgaa gctgttgaac gtgctcggcc
     1801 gctcgaggtc cattgccctg accgagaggg ggtcgatctc ccggggcgtc ccccatcgtt
     1861 cgcgcgctcg gtacgttatt atctcgtagg tccggtcccg gggccgccac gccatggccg
     1921 ccgtcgcccc gatcagccct cttccccctt cccatccgac gagatcggcg ccaaggcgct
     1981 gcagttcttg caggacgtcc gtcttctcca ctatcccccg gacggcctgc cagtagaaac
     2041 gctgcgccgg tttctctgcg ctgatcacca gtccggggct ggccccttcc tcggtgcggg
     2101 cgaggcggcc gatcacatcc tccgccacgg tgagcacgaa ctcggggtcc gcctcctcgg
     2161 ccgtgcgtcc atagctatac actggcatgc cgtccagttc ccctaccatg gtgggctcgc
     2221 ctcgtccgat tcccagccgg aggcagaggg ctccgttccc tcgggtcttc cagggcacag
     2281 ccgggttcaa cctcaccagg cggggaaggc cgatgaggtc gtagaccccg agggctttga
     2341 tcagctcggt ggccaggtac gtggtgcaca tcgattcgag ggaatcggtg tcatcgaggg
     2401 cgacgaacat gctgccccgg cgagagggtt ggggatagaa gaagctttca tttccagggt
     2461 tcgccgatgg gtgaaacgtt gaatagtcgg gtctttaatt cccctttcta tgtctaagcc
     2521 tcttactgtg gaatggggcc tggagatcgc cgtatcgttg atcgtgctcg gcgccgtgct
     2581 tttgatcgtt ggtatcatgg acgttctcca cctctacctg gaccttggtg ggtggggttt
     2641 ttacagcggg gccgtcggcg tgctgctctt gatcgtgggg atcgtatgga tggtctccat
     2701 tctgcaaagg atgcgcaagt tcaaactctt gtttcaggag aagagcaagg cggtcttcgt
     2761 gcgctcgctc gatgagctcg agtacacggt atggaggctt ccgtccaagt acgatgcctt
     2821 gcttttggag aagaggcggg agatggggct gaaataaggg ggggacccct ttccttccac
     2881 tggagacgat ttttaaggaa tgttgcttga ttgatgaggg gtttattgat cggaacggtc
     2941 cgggcgacct ttaactttat tatatatttt atataaaaat cgattagtta gttatcatca
     3001 acagcatccg ttgcagtgga aacccatttc gttccaggca gggagcgcag cctttccgtg
     3061 acccggtcca cctcatcgga agcacatctg aaggccacct tgacatcatc accgtattcc
     3121 accgattcca cgaagaacga atcatagagc caagaaacct ccgaggccgc ccccgcgtcg
     3181 ttccgcattg ccacattgac cgttatcggc ggtcggaaat aggaatcgat gatggcgatc
     3241 aaagcatcga tcccttcacc agtgagcgct gaaacgccga gggtgcacat cggctccaat
     3301 gcatctcgca ccaccatcat ggcctcagcc aggttgtccc gtccctgatc gatcttgttc
     3361 aaagccacaa tcagctcccc tgccatcaac ccccgtccaa gcacatcccg gacggccatc
     3421 accttgtcct ttatgatcgg gatcggctcc gacgagtcca cgaccagcac cacaaggtca
     3481 gcggtgaaca catcctgcag ggtgttccgg aacgattcca ctaggaaggg gggaaggtcc
     3541 ttgaggaagc cgatggtatc ggtgaggagg accttggagg ggcccatccg tctggtggtg
     3601 gtgtccaacg tggagaacat cctgtcggcg atcagcactt cctcgccggt catccgttcc
     3661 atcagcgagg acttgccagc attcgtgtat ccagccaagc agacggcctg gaagcccttg
     3721 tagcgacgca ccccccgtcg catgtcccca tccctggcga gctcttgtaa ccccgcctct
     3781 atccgagcga tcctccttct tatgaggttg taatagacgt ccaccgcata ctccccgccc
     3841 ccgaggaacc ccgggtgctc ccccgccttt gcgctgtgca cccactcccg catcaggggg
     3901 acctggtagc gcagccgggc gagctccacc tgcagcttcg cctcccgccc ctccgccctg
     3961 ctcgtgaaga tgtccaggac caccgcgatc cggtccaggc actccacatt cagcccgctc
     4021 tccaggttga agtgctggga cggcctcagg tccccgttga tcaccagcac gtccactggg
     4081 cgttccctga ggacctcctt gagcgccccg taccgtcccc tgccgatgaa gtacgcccgg
     4141 tccgggaggg ggcgcctctg cacgacctcg tacaggagtt cgatgtctgc cgaccggcag
     4201 agttcctcca tctcggccgt ggcgcggccc aggcttataa gcgctcccgt tcgcacgaac
     4261 gaccatcctc aggcctcaat cccctccgtc cctattacct tatttggtgg cggcccgcgc
     4321 cggcgctcca atggaaacga aggggtgggg gcgttagctg gaaaagcgga aggccggtcc
     4381 actactttcc cgttccccga taaaagtaag gttctagtaa atagaagcgc aatgtccttt
     4441 cccttacttc tgtaaggccc ttatcccgca agaggggcat catggaatcg aacatattca
     4501 ctcagcacct ggaacgcaag cagatcttca agaagaacaa ccgggagatc ctgcgcccct
     4561 cctacatccc ggacgtgctg ccgcaccgcc aggaggagat agactccctt gcctccatcc
     4621 tcgtcaccgc cctgagggga gagcgcccca gcaacatcct gatattcggg aagaccggca
     4681 cgggcaagac cgcctgcgtg aagttcatcg ggaacgagat caagaaggcc ggtggggagg
     4741 cggagccggt caccttcatc tacatgaact gcgaggtggt ggacaccccg tacggggtgt
     4801 tgcagaacat cgggaaccag ttcgtgaagg acttcgatca gcgcatcccg ttcaccggcc
     4861 tgagcatcat gcaggtctac accatgctcc gggacaagct cgacgagcag aaccaggtga
     4921 tcgtcatcgc gctggacgag atcgacaagc tggtgtacaa gagcggggac gacgtcctct
     4981 atcatctctc gaagataaac gacgacctca agaacgcccg ggtgtccatc atcggcatct
     5041 ccaacgacct cctcttcacc gagatgctcg acccccgcgt gcgcagccgc atggagggcg
     5101 agaagatggt gttccctccg tacaacgccg accagctgaa ggacatcctg cggcagcggg
     5161 cggccctcgc cttcgacgac ggggtgctgg aggaggccgt gatcccgctc tgcgccgcgc
     5221 tcgccgccca ggagcacggg gacgcccgcc gcgccctcga cctgctgcgc atcgccggcg
     5281 agatagccga acgggacaac gccgagcggg tggcggagct gcatgtcctg aaggccaaga
     5341 acaagatgga gctggacacc gtgagcgagg ccatccgaac gctgccgacg cagtccaaga
     5401 tggtcctaat gtgcatcgtg atgaacgtgg agaggggggc tggcagactg accaccggcg
     5461 acgtctacga gacatacaag gagatctccc gggtcctggg catggggccg ttgactcaga
     5521 ggaggatcac cgacctcctc tccgagctgg acatgatggg gatcatccac gcccgggtgc
     5581 gctcctttgg acgcgggggg cggaccaagg aaatagacct cggggttccc ctggcggata
     5641 cgaagaaggt gctggaggag gacgacacat tcgccggcct caagcacttc aagcagaaaa
     5701 accaaatgac cctgatatga tcacttccgg cgcagccgtc ccctgagcca cgcccacggg
     5761 tcgatcccgc gccgctccag gaacatgccg gtgaggtcca acgccagcgg cacagcgatc
     5821 accaggacga tgacggccac cagcccggcc ttgctgttct cgggggtgtt ggcgggcatg
     5881 ttgccgttca cccagaggtt tatcagaccg atccacggga tctcggcctt cgccaccccg
     5941 atgacccacc cgtccatcac gggctcgagg cagatggtcg ccgcggactg gtcatagacc
     6001 ccgagccatt gtccatcgga gcgcaccaca ttatggtcgc ccatggtgat gaggccgtcg
     6061 tgggcatact tgagaagggg gaaaaggtcc acgtgcagca gggcggacct gtagccgacg
     6121 tcgtatatct ccacagaccc ggtgagattc caccaccgcc cctcctccag tcccccgttc
     6181 ccccacttgg cgctcggaag cctggccagc gaggggaggt cgaagctgcc cgaggactcg
     6241 ttgaactcaa gacgaagcat cgcccggtga atgacgggcg tgtactcctc ccttccgtag
     6301 cgctcgtaga tgatgacgtc cccgaagtcc ccgaagcttc ggtccccctc cagaaagcag
     6361 tccacgtagg tcctgacgct gacgtcatcg gacgaacgga cgaggacgag gtccccggtg
     6421 tcgatggtcc cgagggcgct ctcagtgtcg ctgtgctgca tgctcgccga ctcgaccacg
     6481 tagaccgggg gccacatgcc cgcgtacacc agcatcgcca ggagcaggat ggccacggtc
     6541 agcaggcctc ctaccaggac cttgtaccgc acgatcacac ccttgatctt cccagaaagc
     6601 tcggacaacg cgttggttac ccactttcct ctaaataatt gtgccgaaga agggattaag
     6661 aacgcgcggc cgcatgcgga tacaatgagc gaacggccgg acaacgacac ttacttcatg
     6721 cgcatggccg tactggtggc gacacgctcc acctgcctgc gccgccaggt cggggccgtg
     6781 atcgtgaagg agaagagggt cctgaccact ggctacaacg gtgccccacg cggcctccgg
     6841 cactgcgagg aggtgggctg cgtccgcaag gagaacaaca tcgagtccgg cacgaggcac
     6901 gagctgtgcc ggggggtgca cgcggaacag aacgcggtca tccagggcgc ctacttcggg
     6961 gtcagcatta aggaggggac ggtatacacc accaacttcc cgtgcgtgct gtgcgccaag
     7021 atcctgctca acgccggcat caaggagatc gtctatctca acgcatacgt ggacgagctc
     7081 tccaagcaga tactggacga ggcggggatc aaggtcaggc ggtacgacag accattataa
     7141 taccattatt ttatatttta ttattaatat ataataatat atcatcaatg cacccgccta
     7201 attcctatat tacttattta tgtcctttat ctgaacattg aaattggatt cccttaagga
     7261 atcaaacaga gccgtcgcag gggtctgaat tttcagcctc agatatccca ataaacaaat
     7321 ggtttatata ctttaattaa attcccagac gtacctttcg gggagacgcc tgtgaacaga
     7381 tctgagattc ttcttcaaat caaggacgcc gaggccaagg cgcagcagat cgtcaaggac
     7441 gcggaggaaa ggcagagggc gaccatagcc gctgccagga aagaggccgc cacccgcatg
     7501 aaagaagccg acgaggcgct caaggccgaa tacgagggca ggctcgcaaa ggagcgtgcg
     7561 agcatcgcct ccgagaggca ggccctcctg aacaagggaa gggaggaggc cgaggccatc
     7621 aggaagaggg ctgaggccaa cgtccccaag gtcatcaccc atctattgca aagcttcgag
     7681 aggactttaa atgttgctgc caatccaaat gactagggtc ctcatcgtag ggtccagagg
     7741 ggcattgaag gacaccatag agctccttta ccggttggag gcggcgcagc tcatagactt
     7801 cagcgcggag gaggaggggt tctcgctcgg cgcccccctc cccgaatcct ctgaggcttc
     7861 ccaaaggctt ctcaagctca ggtccctgga gaaggacatg ggcgtcgacg cggccgaggt
     7921 ccggggacgg atggacgtgg cccagataga gaaggacctt gccgccacca tcgcggagct
     7981 ggaggtggag atcaccagcg tgaccgagtc caggaacaag cttgctggcc ggatcaacga
     8041 gctggaggcc gagagggccg tcctgaaggg cttcctcggt atccccctgg acatggagat
     8101 gtacagcggg tacgaatcct tgagggtcct caccggcaag gtccgggggg accccagcgt
     8161 agctgtgagg gctgccgcgc cccagagcga ggtattcctc tcggacagcg gtgatttcgt
     8221 cgcggtcttc gtggccaagg cccaggccga cgaggcccag agggccttag cgcaattcgg
     8281 cttcaccgag gtgccagtgc ccgccaagag cggctccccc cagcaattgg tggcgagggc
     8341 cgacgaggag cttacggccc tgagggcgag cttggaggag gccgtcagga agctcgagga
     8401 gctgagggaa aggcatgccg ccttcgtcaa ggcagccgac gagcacctta gcatcgtggt
     8461 cgagaaggcc gagacgcccc tgcgtgtcgg gaccacggag tacgccttcg caatggacgc
     8521 ctgggttccc acatcggacg tgcccaaggt ggagaaggcc atcgccgagg ggctgggcgg
     8581 caaggtgcac ttggaggccc tcgcgacagc ccccaggaag gaggcccacg cgtcccacga
     8641 gggcgaggaa aatcctaaat ccct
//
LOCUS       DAOSYN010000037         8587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_7019_length_8587_cov_4.669948, whole genome shotgun sequence.
ACCESSION   DAOSYN010000037 DAOSYN010000000
VERSION     DAOSYN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7019_length_8587_cov_4.669948"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="PLJ11_02755"
     CDS             complement(<1..104)
                     /locus_tag="PLJ11_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO03620.1"
                     /translation="MQALRPLDLRVERGEIVTLLGPSGCGKSSLLNIVA"
     gene            complement(164..694)
                     /locus_tag="PLJ11_02760"
     CDS             complement(164..694)
                     /locus_tag="PLJ11_02760"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOO03621.1"
                     /translation="MDRAVEAVLFAASQPLRASDIADICGVSPDAVRRELRRLAKEYE
                     ERGTAYEIAKIGQRYSLQLRKGYEHYALPFAAMEIPPDVLRTASIIAYNQPILQSDLV
                     KMLGSGVYERVRTLRQMGLVTGGDAAKQGIGIITEARMKQTWDMMVANKLIDPSKVKF
                     HDAWDDRFVRDLKVMP"
     gene            complement(696..1601)
                     /locus_tag="PLJ11_02765"
     CDS             complement(696..1601)
                     /locus_tag="PLJ11_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOO03622.1"
                     /translation="MDAASLDNVLGHLMFHKAMIDEGRGSEKIDRYLALLRSAEEERQ
                     VAGREPLDRALQLVFELVLTNDLDPWDIDLMKFAKLYGQRMHGEEVDFIVAGKLMYMA
                     WSILHLQSREVLSLNEAREELICEGWDMEGMDLFVEQPLPKVELGVPEGVELTEVVRH
                     RCTRPVSLVDLLDAFDAAQQEVEIALNRQRARERLQRAGERFDGKSHSDDQEKDAAEV
                     WARIERCGSGPIALEDLFEGGKEDRIKVFVSLLFLARSGKISLWQEELPYGQIYLEIK
                     LPWEIAKLVDEAEAQALSVHAQMVM"
     gene            complement(1601..5203)
                     /gene="smc"
                     /locus_tag="PLJ11_02770"
     CDS             complement(1601..5203)
                     /gene="smc"
                     /locus_tag="PLJ11_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOO03623.1"
                     /translation="MYLKQIEVENFKSFGKKLTIPMLNGFTAVTGPNGSGKSNISDAI
                     LFVLGPKSSKALRAGKLTDLIFNGGSTKSPANYTRVSLVFDNADRTIPLNEDMVKLTR
                     YVKLSDAGEGYNSYFYVNDRKSTLAEFDYLLASARISAEGYNLVQQGDVTRIVEMSSL
                     ERRKVLDEISGIAKFDEEIARAEGQRLSAQDNIERISIILGELDKQIRQLEQERGAAL
                     KYLEMRDRLALAKARLAHKRREGAEAEIKSIGEQIGSYSKDIEALKLHRSELEAQVRS
                     IEDLYQRKEMELQAKGGAEFLELKERMDVLRIEVARAADLKERSTDQASEVKEGLAAL
                     LEEHKTLLKERDAQQAALASASERLKALEDKVKGLQRDEAKLKGELSSYDSELSTLER
                     AIKERDQRIRERADALHAKVLEKERLERLAEDLRAELETLRQEQEAAEFEVKDLDWRV
                     KELGKQGKDASGSLRSLQEQFHAKKNRESQLARMANELEQAVRNLTREYNHLRAEEEA
                     AANVAKGYTRAVRAVLEARDRGEMKGIHGTIAELANVDPKYEVALNVAAGGRMQSIVV
                     DDDEVASTAITFLKRGDLGRATFLPLNKMLDGKPRGKALLAEKEAEGFAIDLIDFDPK
                     YRAAFWYVFGDTVVVNTLQEARRLMGGVRLVTVGGELIEASGAMVGGTVEGSKVRFGA
                     GRGKADEVAEELRKATEQADKVAMELTAVRQEVADLEAKIHELGGADRGKGIELEGLQ
                     RQLTEQRARSKRVAETLATKQAEREGAIAQAAEVGLAVTKLEEGLAKLREEREASSKR
                     LLEIAPAELSQRLREIQSSVVQASQELAEGRAAHEAIRLQVTELERRLNETAGLEAQY
                     RERGVQLEAQGRAAMERETKLKVELAALKRIEDSMGKEMTALREEKERLVEQKGKAQA
                     EREKVMVRLETANDFLIGLGTKKALAEERLKEIAQEISQTNLVVSYPLPSMDDIRSEM
                     TLCENALSGMGAVNLRAIEDYDEKKARHAELRAETKRLEDQRDQLMALTDQLNERKKV
                     NLLKVFEAVNANFRKVYAELSGGGEAELLLEAPQDPFQGGLVIKARPRNGKVLRLEAL
                     SGGEKSLTALAFIFALQEWQPSPFYLLDEVDMFLDAVNAEMVASRVKKSSATAQFVQI
                     SLRKVTLNKADHIIGVTKQAGGTSSIIFKPNIGDLSELEKEIELPEEKGGRGLA"
     gene            5567..6364
                     /locus_tag="PLJ11_02775"
     CDS             5567..6364
                     /locus_tag="PLJ11_02775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO03624.1"
                     /translation="MLVSIVLKVRNEEDNIADALDSLVVQEGPIEIIVVDGYSTDRTQ
                     EIVQEYASKYPFVHLYVAGSSRGESLNYGLRQAKADIIALTDGDCIANPNWVREIRRC
                     IIERGADMVAGRSINIGLSAWERLDRVELFNKGFDCTWPGCNLAFRREVFETVGGVDP
                     WFVTAEDIDFNIRATNAGFSLVYNPKAIVYNRTRGTFYGFCRQAFWNGAGRKQLTLKH
                     GALWERYDPIKMFRQRMTFWAMVRLGFAWLGYLGFKFFGERPPAEPA"
     gene            6408..7082
                     /locus_tag="PLJ11_02780"
     CDS             6408..7082
                     /locus_tag="PLJ11_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO03625.1"
                     /translation="MQLSVIIPTMNEEESIGQVIDSVRQALAGWDYEILVVDTNSKDR
                     TREIAMEKGAVVVEEPRRGYGRAYKTGFERAKGEVIATLDADCTYPAEEIPALYGMLM
                     ERDLEFITTNRFARLEDGAMTAKHRLGNLALTLMTNVLFGVRIRDSQSGMWVFRRSIL
                     DRLVISADGMPMSEEIKIEAFRKARSLEVPIAYRRRVGEVKLSSWKDGWMNMRFLFEK
                     RFRRQR"
     gene            complement(7073..7600)
                     /locus_tag="PLJ11_02785"
     CDS             complement(7073..7600)
                     /locus_tag="PLJ11_02785"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOO03626.1"
                     /translation="MPPDEGCRRCRLCEGRTTIVLPSGDRRSPVALVGEAPGEQEDLR
                     GEPFVGRAGRTLDRLMAEAGLERGAVLITNTVKCRPPGNRRPKADEMAACRPFLLEEL
                     AGKTFVLALGLSAAEDLLGRRMVMKEVCNRPLQADLGQGTVTVMVTYHPSACVYRPFA
                     KDALREALRTAASYL"
     gene            complement(7603..8124)
                     /gene="pyrE"
                     /locus_tag="PLJ11_02790"
     CDS             complement(7603..8124)
                     /gene="pyrE"
                     /locus_tag="PLJ11_02790"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOO03627.1"
                     /translation="MSEELKRALEDCGAILYGDFTLASGKRSRYYIDIKRATTDPRTL
                     GLIADLMAREIEGETFQRIAGVVLGSVPLAVALSLRTGVPFVMVRKERKDHGTGKLIE
                     GPLSPGERVLVVEDVITSASSAMYAVETLRQAGAEVGTVLAVVDREEGGREALQRVGV
                     GMRALITASHILG"
     gene            complement(8129..>8587)
                     /locus_tag="PLJ11_02795"
     CDS             complement(8129..>8587)
                     /locus_tag="PLJ11_02795"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: protein motif:HMM:NF003114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HOO03628.1"
                     /translation="GRSWKGKRILGDGKTWRGFIGGTCAGISLGIVQLYIAFPFDRLD
                     YFGFGGFPENLGVIVVLAVGSMLGDMAGSFIKRRLDIGRGDKAPLLDQYGFLLCSFLL
                     SLLFYPDWFLRNFWRGDGWISLLAVLIITPVLHRGVNIIGYKLGLKKVPW"
BASE COUNT         1337 a         2966 c         2577 g         1707 t
ORIGIN      
        1 gcgacgatgt tgagcagact cgatttgccg caaccggaag ggccgagcaa ggtgacgatt
       61 tcgccacgct cgacacgcag atcgagcggg cgcagcgcct gcacgccgtt gtcccacgtc
      121 ttctcgacgg cgcgcagttc gatggcgctc acgccgcgct ccgctacggc atcaccttca
      181 ggtcgcgcac gaagcggtcg tcccacgcgt cgtggaactt gaccttgctc ggatcgatga
      241 gcttgttggc gaccatcatg tcccaggtct gcttcatgcg cgcctcggtg atgatgccga
      301 tgccctgctt cgcggcgtcg ccgccggtga ccagccccat ctggcgcagc gtcctcaccc
      361 gttcgtacac cccggagccg agcatcttca ccaggtcgct ttggaggatc ggctggttgt
      421 aggcgatgat ggaggcggtc ctgagcacgt ccggcgggat ctccatggcc gcgaagggga
      481 gggcgtagtg ctcgtacccc ttcctgagct ggagggagta ccgctggcct atcttggcga
      541 tctcgtaggc ggtgccgcgc tcctcgtatt ccttcgccag ccgcctgagc tcccttctca
      601 cggcgtccgg gctgacgccg cagatgtccg cgatgtccga ggcccggagg ggctgggacg
      661 ccgcgaacag gacggcctcc accgcccggt ccacctcaca tcaccatctg ggcgtggacg
      721 gagagggcct gggcctccgc ttcgtccacc agtttggcta tctcccaggg cagcttgatc
      781 tccaggtata tctggccata gggaagctcc tcctgccaga gggagatctt gccgctgcgg
      841 gcgaggaaca gcagggagac gaacaccttg atgcggtcct ccttgccgcc ctcgaacagg
      901 tcctccaggg cgatgggccc ggagccgcag cgctcgatgc gcgcccatac ctccgcagcg
      961 tccttctcct ggtcatcgct gtggctcttg ccgtcgaacc tttccccggc cctctgcagc
     1021 ctttcccggg ccctctggcg gttcaatgct atctccacct cctgctgggc cgcatcgaag
     1081 gcgtcgagca ggtccacgag cgacacgggg cgggtgcagc ggtggcggac gacctcggtc
     1141 agctccacgc cctccgggac ccccagctcg accttcggaa ggggctgctc cacgaacagg
     1201 tccatgccct ccatgtccca cccttcgcag atgagctcct cccgcgcttc gttgagggag
     1261 agcacctccc gggactgcag gtgcaggatg ctccaggcca tgtacatcag tttgccggcg
     1321 acgatgaagt cgacctcctc gccgtgcatc ctctggccat agagcttggc gaacttcatt
     1381 agatcgatgt cccaggggtc caggtcgttg gtgagcacca gctcgaacac gagctgcagg
     1441 gcgcggtcca acggctccct cccggcgacc tgcctctcct cctcggcgga gcgcagcagg
     1501 gccaggtagc ggtcgatctt ctccgagccc cgcccttcgt cgatcatagc cttatggaac
     1561 atcagatgcc ccaacacatt gtccaagctc gcagcgtcca tcatgccaat cccctcccgc
     1621 ccttctcctc gggcagctcg atctccttct ccagctcgct caggtcgccg atgttcggct
     1681 tgaatatgat gctcgaggtc cccccggcct gcttggtgac cccgatgatg tggtccgcct
     1741 tgttcagggt gaccttcctc agggatatct gcacgaactg ggccgtggcg ctgctcttct
     1801 tgacccggct cgccaccatc tcggcgttca ccgcgtccag gaacatgtcc acctcgtcca
     1861 gcagatagaa aggggagggc tgccactcct gcagcgcgaa gatgaaggcc agcgcggtca
     1921 ggctcttctc ccccccgctc aacgcctcca gtctgagcac cttgccattg cggggccggg
     1981 ccttgatcac cagcccgccc tggaacgggt cctgcggggc ctcgaggagg agttccgcct
     2041 ctcccccgcc cgagagctcc gcgtacacct tgcggaagtt ggcgttgacc gcctcgaaca
     2101 ccttcagcag gttgaccttc ttcctctcat tgagctggtc ggtgagggcc atcaactggt
     2161 ccctctggtc ctccagcctc ttggtctcgg cgcgcagctc ggcatgccgg gccttcttct
     2221 cgtcatagtc ctcgatggcc ctgaggttca cggcccccat gccgctcagg gcgttctcac
     2281 agagcgtcat ctcggagcgg atgtcgtcca tcgagggcag cggatagctg accaccaggt
     2341 tggtctggga gatctcctgc gctatctcct tcaacctctc ctcggccaag gccttcttcg
     2401 ttcctagccc tatcaggaag tcgttggccg tctccagcct taccatgacc ttctcccgct
     2461 cggcctgggc cttccccttc tgctccacca gcctctcctt ctcctcccgg agggctgtca
     2521 tctccttgcc catcgaatcc tcgatcctct tgagggccgc cagttcaacc ttcagcttcg
     2581 tctccctctc catggcggcc cttccctgcg cctcgagctg tacgcccctt tccctgtact
     2641 gggcctcgag cccggccgtc tcgttcaggc gtctctccaa ctcggtgacc tgaagccgga
     2701 tggcctcgtg cgccgcgcgt ccctctgcca gttcctgcga cgcctggacc acggaggact
     2761 gtatttccct caacctctgc gagagctcgg cgggggctat ctccaaaagc cgtttggagc
     2821 ttgcctccct ctcctccctg agctttgcga gcccttcctc cagcttggtg accgccaggc
     2881 cgacctcggc cgcctgggct atggccccct ccctctcggc ctgtttggtc gcgagggtct
     2941 cggcgacccg ctttgagcgg gccctctgct ccgtcagctg ccgctgcagc ccctcgagct
     3001 cgatgccctt cccccggtcg gcccctccga gctcgtggat cttggcctcg aggtcggcga
     3061 cctcctgccg cacggccgtg agttccattg cgaccttgtc cgcctgctcg gtggccttgc
     3121 gcagctcctc tgccacctcg tcggcctttc ccctgcctgc gccgaagcgc accttgctgc
     3181 cctcgaccgt ccccccgacc atggccccgg aggcctcgat gagctccccg cccacggtca
     3241 ccagtcgcac cccgcccatc aagcggcggg cctcctggag ggtgttgacg accacggtgt
     3301 ccccgaacac gtaccagaat gccgcccggt acttcgggtc gaagtcgatg agatcaatgg
     3361 cgaacccttc ggcctccttc tcggccagga gggccttgcc cctgggcttt ccgtccagca
     3421 tcttgttcag gggcagaaag gtcgcgcggc cgaggtcgcc cctcttcagg aaggtgatgg
     3481 ccgtggaggc cacctcgtca tcgtcgacca cgatggactg catgcgcccc cccgccgcca
     3541 cgttcagggc gacctcgtac ttgggatcga cgttggcaag ctccgcgatg gtcccgtgga
     3601 tgcccttcat ctccccgcgg tcccgggcct cgagcaccgc cctcaccgcc ctggtgtacc
     3661 ccttggccac gttcgccgcg gcctcctcct ccgccctcag gtgattgtac tcccgggtaa
     3721 ggttcctcac cgcctgctcc agctcgttgg ccatcctggc cagctgggat tcccggttct
     3781 tcttggcgtg gaactgctcc tgcagcgatc gcagggagcc gctggcgtcc ttgccctgct
     3841 tgcccagctc cttgaccctc caatccaggt ccttgacctc gaactccgcg gcctcctgct
     3901 cctgtcgcag cgtctccagc tcggccctca ggtcctcggc cagcctttcc agcctttcct
     3961 tctcaaggac cttggcgtgc agggcatcgg ccctctcccg gatcctctgg tcccgctcct
     4021 tgatcgccct ctccagcgtg gatagctcgg aatcgtagga gctgagttcg cccttcagct
     4081 tggcctcgtc cctctgcagt cccttgacct tgtcctccag ggccttgagc ctttcgcttg
     4141 ccgaggccaa tgccgcctgt tgggcgtccc tttccttgag cagggtcttg tgctcctcga
     4201 gcagggccgc gagcccttcc ttgacctcgg aggcctggtc cgtggagcgc tccttcaggt
     4261 cggcggcccg ggcgacctct atccgaagga cgtccatccg ctccttcagc tccaggaact
     4321 ccgctccgcc cttcgcctgg agctccatct cctttctttg atagaggtcc tcgatggagc
     4381 ggacctgcgc ctccagctcg gagcggtgga gcttcagggc ctcgatgtcc ttggaatagg
     4441 agcctatctg ctcccctatg ctcttgatct ccgcctccgc cccctccctg cgcttgtggg
     4501 ccaggcgggc cttcgccaag gccaggcggt ccctcatctc caggtactta agggccgccc
     4561 ccctctcctg ctccagctgg cgtatttgct tgtccagctc gcccaggatg atggagatgc
     4621 gctcgatgtt gtcctgggcc gagagcctct gcccctcagc cctggcgatc tcctcgtcga
     4681 acttcgctat ccctgatatc tcgtcgagca ccttccgccg ttcgagggag gacatctcca
     4741 cgatgcgggt cacgtccccc tgctgcacga ggttatagcc ctcggcgctg atgcgagcgc
     4801 tggcaagcag gtagtcgaac tcggccaggg tggacttgcg gtcgttcaca tagaaatagg
     4861 agttatagcc ctctccggca tcggacagct tgacgtacct ggtgagcttg accatatcct
     4921 cgttcagggg gatggtgcgg tcggcgttgt caaagaccag ggacaccctc gtgtaattgg
     4981 cgggggactt ggtgctgccg ccattgaata tgaggtcggt gagcttgccc gcgcgcaggg
     5041 ccttggagct cttcggaccc aggacgaaaa ggatggcgtc ggatatattg gacttgccgg
     5101 agccgttggg gccagtgacg gcggtgaacc cgttcagcat gggtatggtc agcttcttgc
     5161 cgaatgactt gaagttctct acctctatct gctttaggta catgcaacac tacctggggt
     5221 gcgaaggtgc tctctcccac ccgccttcgc tcgagcgcgt cccatcccga gtccgacata
     5281 tgtcggacat attatgtata ttatcctccg tatatatatt ttgtcaccat agggacgatg
     5341 gccagagacc ggaccgccat tggatccgag gcaggttgag ggcctgccta ttccacgaga
     5401 cagccattag gcctcttcca ccagggcttg gtcctctgcg acgggctttg tgcgacggaa
     5461 tgtctgacag agttccttct tcgcgccgaa gggcggcgcc tccgggccag cccatgcccg
     5521 actggccctc aaggggcttt tatactgtaa cacagatgga ggggggatgc tcgtatccat
     5581 cgtcctcaag gttcggaacg aggaggataa catcgccgat gccctggact cgttggtggt
     5641 gcaggagggg cccatcgaga tcatcgtcgt ggatggttac agcacggacc gcacccagga
     5701 gatcgtgcag gagtacgcgt ccaagtatcc cttcgtgcac ctgtacgtgg cggggagcag
     5761 ccggggagaa agcctgaact acgggctgag gcaggccaag gcggacatca ttgcgttgac
     5821 ggacggcgat tgcatcgcca accccaattg ggtgagggag atccgcaggt gcatcatcga
     5881 acggggcgcg gacatggtgg cgggcaggtc catcaacatc ggcctgagcg cctgggagag
     5941 gttggaccgg gtggaactgt tcaacaaggg ctttgactgc acctggcccg gatgcaacct
     6001 ggccttccgg agggaggtct tcgagaccgt ggggggcgtg gacccctggt tcgtgaccgc
     6061 cgaggacatc gatttcaaca tcagggccac gaacgctggt ttcagcctgg tgtacaaccc
     6121 caaggccatc gtgtacaacc gcacccgggg cacgttctac ggcttctgcc gccaggcctt
     6181 ctggaacggg gcgggcagga agcagctgac cctgaagcat ggcgccctct gggaaaggta
     6241 tgacccgatc aagatgttcc ggcagcggat gaccttctgg gcgatggtgc gcctggggtt
     6301 cgcttggctt gggtacctgg ggttcaagtt cttcggggag cggccaccgg ccgagcccgc
     6361 ctgaaagcat tattagctat atccccttct cctccagagg cggaagcttg cagctaagtg
     6421 tcatcatccc caccatgaac gaggaggagt ccatcgggca ggtcatcgac agcgtgcgcc
     6481 aggccctcgc gggatgggac tacgagatcc tggtggtgga cacgaactcc aaggaccgca
     6541 ccagagagat agccatggag aagggcgcgg tggtggtgga ggagccccgc cgaggctacg
     6601 gccgagcgta caagacgggg ttcgagcggg cgaagggaga ggtcatcgcc accttggacg
     6661 cggactgcac ctaccccgcg gaggagatcc ccgccttgta cgggatgctc atggaacggg
     6721 acctcgagtt cataacgacc aaccgcttcg cccggctgga ggatggggcc atgaccgcca
     6781 agcaccgcct gggcaacctc gccctgaccc tgatgaccaa cgtcctcttc ggcgtgcgga
     6841 taagggattc ccagagcggg atgtgggtgt tccgccggag catcctggac cggctggtca
     6901 taagcgctga cgggatgccg atgtcggagg agatcaagat cgaggcgttc cgcaaggcgc
     6961 gcagcctgga ggtccccatc gcctaccggc ggagggtggg tgaggtcaaa ctatccagct
     7021 ggaaggatgg ctggatgaac atgcgcttcc tctttgagaa gcgcttccgg cgtcagaggt
     7081 aggaggcggc ggtccgcagc gcctcccgca gcgcgtcctt ggcaaagggg cggtagacgc
     7141 aggccgaggg atggtaggtc accatgacgg tcacggtgcc ctggcccagg tccgcttgca
     7201 gcggccggtt gcagacctcc ttcatgacca tcctccgccc gaggaggtcc tccgccgcgc
     7261 tgagcccgag ggccaggacg aaggtctttc cggccagctc ctcgagcagg aacggccggc
     7321 aggccgccat ctcgtctgcc ttcggcctgc ggttcccggg aggccggcac ttcactgtgt
     7381 tcgttatgag gacggccccg cgctccagcc ctgcctcggc catgagcctg tccaaggtcc
     7441 ttccggcccg gcccacgaag ggctcgcccc tcaggtcctc ctgctccccg ggggcctccc
     7501 ccaccagcgc gacgggcgac ctgcggtccc cgctcggcag gacgatggtg gtccgcccct
     7561 cgcacaggcg gcaccggcgg catccctcgt cgggaggcat cttcacccca ggatgtggct
     7621 ggcggtgatg agcgccctca tcccaacgcc caccctttgc agggcctccc ttccgccctc
     7681 ctcccggtcc accacggcca ggaccgtgcc cacctccgcc ccggcctggc gcagcgtctc
     7741 gacggcgtac atcgcggagc tggcggaggt gatgacgtcc tccaccacca gcaccctctc
     7801 cccggggctc aggggacctt ctatgagctt gccggtgccg tggtccttcc tctccttccg
     7861 gaccatgacg aacgggaccc cggtcctcag ggagagcgcc accgcgagcg ggacggagcc
     7921 cagcaccacc ccggcgatgc gttggaacgt ctccccctcg atctcccttg ccatcaggtc
     7981 ggcaatcagc cctagcgtcc tgggatcggt ggtcgctctc ttgatgtcga tgtagtacct
     8041 gctcctcttt cccgacgcca gggtgaaatc cccgtagagg atggcgccgc agtcctccaa
     8101 cgccctcttc agttcctcgc tcatgtgatc accaggggac cttcttcagc cccagcttgt
     8161 atcctatgat gttcaccccg cggtgcagga ccggggtgat gatcagcacg gccagcaggg
     8221 agatccagcc atctccgcgc cagaagttcc tgaggaacca gtcgggatag aacagcaggg
     8281 agagcaggaa ggagcagagc aggaagccgt actgatccag cagcggggcc ttgtcccccc
     8341 ttcctatgtc caggcggcgc ttgatgaagg agccggccat gtccccgagc atcgagccta
     8401 ctgccaggac gacgatcacc cccaggttct cggggaaccc cccgaagccg aagtagtcga
     8461 gacgatcgaa cgggaacgcg atgtacagct gcactatccc gagggatatg ccggcgcatg
     8521 tcccgccaat gaatccccgc caggtcttgc catccccgag gatccgtttt cccttccatg
     8581 agcggcc
//
LOCUS       DAOSYN010000038         8551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_7061_length_8551_cov_4.222811, whole genome shotgun sequence.
ACCESSION   DAOSYN010000038 DAOSYN010000000
VERSION     DAOSYN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7061_length_8551_cov_4.222811"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLJ11_02800"
     CDS             <1..707
                     /locus_tag="PLJ11_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOO03629.1"
                     /translation="RWRFVCVAKKFGAVEKDADYRNVGFARLVEAYESTPLMQEAIDK
                     VLWEDFDVARTVEVVRAIGAGEIDIALCGLSPIGRAGLTQSKELITPQRADHSILMAL
                     KRRLEEESLYMSCLNCGVQWRMRPKDAGRRVLCPNCGGQMIAALNGYNRDTIKLIRKK
                     ALSEEEEKEVRRLYKNASLVQSNGRRALLAFAGRGIGADTAARILSSFSEDEEDLLRD
                     ILNAEITYARTKRFWD"
     gene            complement(704..1330)
                     /locus_tag="PLJ11_02805"
     CDS             complement(704..1330)
                     /locus_tag="PLJ11_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOO03630.1"
                     /translation="MRCGKIVCVGQNYREHVREMRSAEPTEPVLFLKPATALIGDGDP
                     IRIPAGIGTVHHEVELALIIGTGGRDIEERKALAHVSHAAVFNDVTARDMQAAARRAG
                     LPWALAKGIDTFAPMGAPVPLERIQDVHALGLELRVNGERRQKGSTAQMIFPPEALIS
                     YISRFMTLEPGDIIATGTPSGVGPLVPGDVVEAMVDEAGAVRNPVEGR"
     gene            complement(1333..1575)
                     /locus_tag="PLJ11_02810"
     CDS             complement(1333..1575)
                     /locus_tag="PLJ11_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03631.1"
                     /translation="MLTYITIIFSADGGRPSEIFNALHNMGFEPTTGNYDGVFKWDRK
                     ATLDDAIYLADRVHLTLKGTGALFKLETVAEVHDKC"
     gene            complement(1609..2232)
                     /locus_tag="PLJ11_02815"
     CDS             complement(1609..2232)
                     /locus_tag="PLJ11_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOO03632.1"
                     /translation="MSKNWMQERRRDYYYRKAKQLDYRSRASFKLMQLDERFSLFKEG
                     MTVVDLGAAPGGWLQVAAERVGPRGRVIGVDLQPIEPLEGVRTIKGDVRRKEVREELL
                     SLTGGRVDMVMSDMSPNISGSYSMDHARSIELCEIALAFALETLSPGGSLVFKMFEGD
                     MSRDLVTEVQRHFATVKRYSPQASRASSSEIYVVAKGFGKGKGGQSG"
     gene            2322..3347
                     /gene="purM"
                     /locus_tag="PLJ11_02820"
     CDS             2322..3347
                     /gene="purM"
                     /locus_tag="PLJ11_02820"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOO03633.1"
                     /translation="MNESEWTYARAGVDIDRKSQAISALVGRLRYRRTGRGRMIELPG
                     QFTGLMDMGDHILTLATDGVGTKLLVAEALAKWDTVGIDCMAMNVNDTICVGAEPIAF
                     VDYIALDEPRPEVTEQIGIGLDRAAEMANVDLVGGEIAVLPEMVRGLDLSGTALGLLD
                     KERMVTGAEVQEGDAIIGLLSSGVHSNGMTLARKVLAAANVSLHDEVDGLDKSVGMEL
                     LTPTEIYVRDVLRVVGSVKVTGMVDVTGGGLKNFVRLRKGLEYRITDPIEPQPIFRVL
                     QRLGRITDQEMYRTFNMGMGFGIVLRPEGVEEALSLLKGRAQLVGRVVKGSGCGIPGL
                     KVHYDTY"
     gene            complement(3344..4006)
                     /locus_tag="PLJ11_02825"
     CDS             complement(3344..4006)
                     /locus_tag="PLJ11_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03634.1"
                     /translation="MDDARLRFRVKTWGLLSFLVLTASVALIFYEGPFLELNGHRAGV
                     GQILWVPGLIGSCVCLWLLLTLSGAHSGLSVPLALGALAGAELCALFLPVSEVSGLYW
                     EKGNGLVPAITFASLALTSVIWTATRLIDPSRTSRHTYTVALAAVLVIGFLLSYKFFW
                     SLQGVMATGLAASLLWGMAYDPRLHPYGRKQKGRGHPGALLLATFLGLPLLLIGLPYL
                     LL"
     gene            complement(4033..4596)
                     /locus_tag="PLJ11_02830"
     CDS             complement(4033..4596)
                     /locus_tag="PLJ11_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF531 family protein"
                     /protein_id="HOO03635.1"
                     /translation="MERRVGRATIAIYNSYDPKAFREAHRRIMARAGPLALAYDLNLA
                     TLGFPFPEDLRLPVELAEWVASTTSIGEDGSYLRELASKGRFQNFPYPSKGLPPQLGL
                     PVLTTCAPDPSKRMELEQLTSLLLEGQSACLVFGAGPKGVPKDVREMARHHLDITFGG
                     HSLETCTALGAVAGALAHSLRWRQKQV"
     gene            complement(4601..4945)
                     /locus_tag="PLJ11_02835"
     CDS             complement(4601..4945)
                     /locus_tag="PLJ11_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOO03636.1"
                     /translation="MYIGGGVPKNYIQQTEVICETLGYDKGGHHYAVQITQDAPQWGG
                     LSGCTFEEAQSWGKINRNATKSVAYVEASIGLSLMVGYLLQSGAWKGRKRLKYTWKDD
                     MLTRVSKAPLGL"
     gene            complement(4840..5658)
                     /locus_tag="PLJ11_02840"
     CDS             complement(4840..5658)
                     /locus_tag="PLJ11_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOO03637.1"
                     /translation="MVSKKDVLSEKVKQIDFDKIVTVKDLVEAYKDSSIQSRALATCA
                     TVYEKALKDRSRPTVILGLAGPLVAAGLRKVIADMVKFGIVDAIVTIGAIPYQDFYQA
                     RGYHHYKCSPHCDDLALRELFIDRIYDTLVDEERFRETDEVIGNIAGELEPRAYSSRE
                     FMKVLGERIEDEGSILYNAAKYGVPVFVPALNDSSIGIGLTGLYYKRRMANEPFMRID
                     QSGTTGSLPRSRYTPTRPRSSTSAAGSRRTTFSRPRSSARPWAMTRAAITMPFR"
     gene            complement(5706..5876)
                     /locus_tag="PLJ11_02845"
     CDS             complement(5706..5876)
                     /locus_tag="PLJ11_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03638.1"
                     /translation="MEKRVHIKIDRNSDFTLREVLKKIEEIQAENPDLDVFFDGDDYA
                     ICSRPRKVKGRT"
     gene            6188..7909
                     /locus_tag="PLJ11_02850"
     CDS             6188..7909
                     /locus_tag="PLJ11_02850"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOO03639.1"
                     /translation="MTKGKDRSVPRAEQGSAEQVSPTADQAEVERLSRWLDGEGQGSA
                     LLTWLGEGGDFASEDELAELKDRVGRYEMELAAVRALLAVDGKEGEGTAQRLQALIQE
                     RDALKEVASKAVAPNGGNGLAENREKELLERYHAEIKDKDEQWKRAEGELSARIATLE
                     KELAKTKIELKLKDDELTVLRNGGGTVNADLLGRLESLQSKEREFLMLKQEADDLRVR
                     LKEKEEELDRIREAITYKEDELIRREEDLMYREKVYTAERKRLEEMKRETGGLEEAEL
                     MKKLEALKAEVATKEDELRAKEKYVLARQEELRLREQGLIEDEITYREKERALELQER
                     KVKTGIPRFDDLLLGGIPIGSNIMIHGPPFIGKEVMANTFLMEGLRKGIPALIVLTDR
                     TPKDVRDEMRYIMAGFEEYEKLGLVRYVDTYSRAMGDDASDPYTVYIEQPNDHERLGE
                     TIDKLAKEFKEKHEYYRLVFRSVSTLIAYSDPNSAFRFLSPQCGRRKKDQAVSMYIVE
                     KGMHSEQELQMLGSIMDGMVDFKVDQLKTFFAIRGIGDVQSRAYIRYTATKSALTIGS
                     FALDHIR"
     gene            complement(7906..8331)
                     /locus_tag="PLJ11_02855"
     CDS             complement(7906..8331)
                     /locus_tag="PLJ11_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03640.1"
                     /translation="MQIRSFEITAIEAKRFAKSGEKVSNIKIENNSAITQISKADEGL
                     HQIDFRFTANYTGMGYIKVEGHLMVADLGESVMEDWRRTNNLPVDDANALHNAIVSNC
                     MVTALIIAREIKLPAPIPMPRINVQKRPEARPSNGVEVA"
     gene            complement(8408..8514)
                     /locus_tag="PLJ11_02860"
     tRNA            complement(join(8408..8446,8478..8514))
                     /locus_tag="PLJ11_02860"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8479..8481),aa:Phe,seq:gaa)
BASE COUNT         1616 a         2548 c         2878 g         1509 t
ORIGIN      
        1 cccggtggcg ctttgtctgc gtggcgaaga agttcggagc ggtggagaag gacgcggact
       61 acaggaatgt tggcttcgcc cggctggtgg aggcctacga gagcaccccg ctcatgcagg
      121 aggccatcga caaggtgcta tgggaggact tcgacgtcgc gcggaccgtg gaggtggtgc
      181 gggccatagg ggccggggag atcgacatcg ccctctgcgg cctgtccccc ataggccggg
      241 cgggcctcac ccagtccaag gagctgatca cgccgcagag ggccgaccac tccatactga
      301 tggctttgaa gaggaggctg gaggaggaga gcctttacat gtcctgcctc aattgcggcg
      361 tgcagtggag gatgaggccc aaggatgccg ggagacgcgt gctctgcccc aactgcgggg
      421 ggcagatgat cgccgccctc aatggctaca accgggacac gatcaagctg atcaggaaga
      481 aggccctgag cgaggaggag gagaaggagg tccggcggct gtacaagaac gcgtccctgg
      541 tgcagagcaa cggacgcagg gccctgctgg ccttcgccgg ccggggaatc ggggcggaca
      601 ccgcggcccg catcctgtcc agcttcagcg aggatgagga ggaccttctc agggatatcc
      661 tgaacgcgga gatcacctat gcccggacca agcgcttctg ggactagcgc ccctcgaccg
      721 ggttgcgcac cgcccctgcc tcgtccacca tggcctccac aacatctccg ggcaccaggg
      781 ggccgacccc gctgggcgtc ccggtggcga tgatgtcccc aggctccagg gtcatgaacc
      841 gtgatatgta ggagatcagc gcctcgggcg ggaagatcat ctgggcggtg cttcccttct
      901 gccggcgctc cccgttcact ctcagctcta ggcccagggc gtgcacgtct tggatgcgct
      961 cgagggggac gggggcaccc atcggagcga acgtgtcgat ccccttggcc aaggcccagg
     1021 gcagaccagc cctgcgggcc gcggcctgca tgtcgcgggc ggtgacgtcg ttgaagacgg
     1081 ccgcatgtga gacgtgggcc agtgccttcc tttcctctat gtccctcccc ccggtgccga
     1141 tgatcagcgc cagctccacc tcgtgatgca ccgtgccgat cccagccggg atgcggatcg
     1201 ggtccccgtc cccgatgagc gccgtggccg gcttcaggaa gagcaccggc tcggtcggct
     1261 cggcggacct catctcccgg acgtgctccc ggtagttctg cccgacgcag acgatcttcc
     1321 cgcaccgcat ggtcagcact tgtcgtggac ctcggccacg gtctccagct tgaacaatgc
     1381 cccggtgccc ttgagggtca ggtgcacccg gtcggccagg tagatcgcgt cgtccagcgt
     1441 ggccttcctg tcccacttga acaccccgtc gtagttgccc gtggtgggct cgaaccccat
     1501 gttgtgcagg gcgttgaaga tctcggaagg tcggcccccg tccgcgctga aaataatagt
     1561 gatgtaggtc agcatcgaat gtggcatgac catgggggga taaaaagatt agccgctttg
     1621 gcctcccttc cccttcccga agcccttggc gacgacgtag atctcggagc tgctcgccct
     1681 ggaggcctgg ggggagtagc gcttgacggt ggcgaagtgc ctctgcacct ccgtcacaag
     1741 gtcccggctc atatcgccct cgaacatctt gaagaccagg ctgccccctg gggagagcgt
     1801 ctccagggcg aaggccaatg cgatctcgca cagctctatg gagcgggcgt ggtccatgga
     1861 atagcttccc gagatgttcg gggacatgtc ggacatgacc atgtccacgc gccctccggt
     1921 gagggagagg agctcctccc ggacctcctt cctgcgcacg tcgcccttga tggtccgcac
     1981 gccctccagg ggctcgatgg gctgcaggtc cacccctatc acccggcccc ggggccccac
     2041 gcgctccgcg gccacttgga gccagcctcc cggcgccgcc ccaaggtcga ccacggtcat
     2101 gccctccttg aacaggctga agcgctcgtc gagctgcatg agcttgaagg aggcccggct
     2161 gcggtagtcc aattgcttgg ccttcctgta atagtaatcg cgccttcgct cctgcatcca
     2221 gttcttggac atcctgccct gcccaacccc ttgcgggtta aaaaacattg cctgaccgat
     2281 tctattttat cgcctggggg gattcggcat tgggagcgcg catgaacgag agcgagtgga
     2341 catacgccag ggccggggtg gacatcgacc gcaaatcgca ggccatttcg gccttggtcg
     2401 gccgcctccg gtaccggcgc accggccggg ggaggatgat cgagctgcca gggcagttca
     2461 cgggcctgat ggacatgggc gaccacatcc tgaccctcgc cacggacggc gtgggcacga
     2521 agctgctggt cgcggaggcg cttgccaagt gggacacggt gggcatcgac tgcatggcca
     2581 tgaacgtcaa cgacaccatt tgcgtggggg cggagccgat cgccttcgtg gactacatcg
     2641 ccctggacga gcccaggccg gaggtcaccg agcagatcgg catcgggctg gaccgggcgg
     2701 cggagatggc caacgtggac ctggtgggcg gggagatagc cgtgctcccg gagatggtca
     2761 gaggcctgga cctctcaggc accgccctcg gcctgcttga caaggaaagg atggtcaccg
     2821 gcgccgaggt ccaagagggc gacgccatca tagggctcct ctcctcgggg gtgcactcca
     2881 acggcatgac ccttgcgcgg aaggtgctcg cggccgccaa cgtctcgctg catgacgagg
     2941 tggatgggtt ggataagagc gtggggatgg aactcctcac gccgaccgag atctacgtcc
     3001 gggacgtgtt gagggtggtc ggctcggtca aggtgaccgg gatggtggac gtcacgggcg
     3061 gggggctgaa gaacttcgtg cgcctcagga aggggctgga gtaccgcatc accgacccga
     3121 tcgagccgca gccgatattc agggtgctcc aacgcctcgg ccggatcacc gatcaggaga
     3181 tgtacaggac gttcaacatg ggaatgggct tcgggatcgt tctgaggccg gagggcgtgg
     3241 aggaggcgct ttccctcctg aagggcaggg cccagctcgt cgggagggtc gtcaagggct
     3301 ccggctgcgg gatacccggg ctgaaggtcc actatgacac ctattagagc agcaggtacg
     3361 gaaggccgat gaggagcaac ggaagcccga ggaacgtcgc gaggagcagc gcgcccgggt
     3421 ggccccgccc cttctgcttg cggccgtagg gatgcaggcg ggggtcgtag gccatgcccc
     3481 agagcaggga ggccgcgagc ccggtggcca tcaccccctg cagggaccag aagaacttat
     3541 aggagagtag gaacccaatc acaagcaccg ccgccaatgc caccgtgtac gtgtgacggc
     3601 tggtgcggga agggtcgatg agccgggtgg cggtccagat cacggaagta agcgccaggg
     3661 aggcgaaggt gatggccggc acaaggccgt tgcccttttc ccagtagagg ccggagacct
     3721 cgcttaccgg taggaagagc gcacagagct cggccccggc aagggcgccg agggcgaggg
     3781 ggaccgacag gccgctgtgg gcgccgctca gggtcagcag cagccagagg cagacgcagg
     3841 aaccgatcag ccctggcacc cataggatct ggccgacccc tgcccgatgg ccgttcagct
     3901 cgaggaacgg cccctcatag aagatgaggg caacggaggc cgtgagcacc aggaaggaga
     3961 gcagccccca tgtcttcacc ctaaaccgca gccttgcatc gtccacgtga tgggataacc
     4021 tattggcgtg cattaaactt gcttctggcg ccatcgcagg gaatgggcca gcgccccggc
     4081 caccgccccc agggcggtgc atgtctccag cgaatgcccg ccgaacgtta tgtccaggtg
     4141 atgccgggcc atctcccgga cgtccttggg cacgcccttc ggaccagcgc caaagaccag
     4201 gcaggcgctc tgcccctcca gcagaaggga ggtcagctgt tccagctcca tccgcttgga
     4261 ggggtccggg gcgcaggtcg tgagcactgg caggcccagc tggggcggca gccccttgga
     4321 gggatagggg aaattctgaa agcggccctt ggatgccagc tccctcaggt atgagccgtc
     4381 ctcgccgatc gatgtggtgg aggccaccca ttccgccagt tcgacgggca ggcgcaggtc
     4441 ctccgggaag gggaagccca gggtggccag gttgaggtcg taggccaggg cgaggggtcc
     4501 ggccctggcc atgatccgac ggtgggcctc ccggaaggcc ttggggtcgt aggagttata
     4561 gatggcgatg gtggcccggc ccaccctgcg ctccatgcac tcagagcccc agcggggcct
     4621 tgctgaccct ggtcagcatg tcgtccttcc atgtgtactt cagccgcttc cggcccttcc
     4681 aggccccgga ctgcaggagg tagccgacca tcagcgaaag cccgatggag gcctcgacgt
     4741 aggccacgga cttcgtggcg ttgcggttga tcttgcccca ggactgcgcc tcctcgaagg
     4801 tgcagccgct caggcccccc cattgggggg cgtcctgggt tatctgaacg gcatagtgat
     4861 ggccgccctt gtcatagccc aaggtctcgc agatgacctc ggtctgctga atgtagttct
     4921 tcgggacccc gccgccgatg tagatgaccg cggtcttgtc ggagtgtatc ttgatctggg
     4981 taagctccca gttgtcccgg attggtcgat gcgcatgaac ggctcgttgg ccatcctcct
     5041 tttgtagtac aggccggtga ggccgatgcc tatggaggaa tcgttgaggg cggggacgaa
     5101 cacggggacc ccgtacttcg cggcattgta caggatcgag ccctcgtctt cgatcctctc
     5161 accgaggacc ttcatgaact cccggctgct gtacgccctt ggctccagct cgccagcgat
     5221 gttcccgatg acctcgtcgg tctcacggaa cctttcctcg tcgaccaggg tgtcgtagat
     5281 acggtcgatg aagagctcgc gcagggcgag atcgtcgcag tggggggagc atttataatg
     5341 gtgatagccc cgggcctggt agaagtcctg gtacggaatc gccccgatgg tgacgatggc
     5401 gtccacgatc ccgaacttga ccatgtcggc gatgaccttg cgcaggccgg cggccacgag
     5461 cggcccggcc agcccgagga tgaccgtagg gcgggacctg tccttcaagg ccttctcata
     5521 gaccgtggcg catgtggcca gggcgcggct ctggatggac gagtccttgt acgcctccac
     5581 caggtccttc acggtgacta tcttgtcaaa gtctatctgc ttgaccttct cgctcagaac
     5641 atccttcttg cttaccaatt ccaaccctcg cttaaatttt aatctgaagc taccctggtc
     5701 aggcctcagg tccggccctt gaccttcctg ggcctgctgc agatggcata gtcgtcccca
     5761 tcaaagaaga cgtcgaggtc ggggttctcc gcctggatct cctcgatctt cttcaagacc
     5821 tcgcgcagcg tgaagtcgct gttgcggtcg atcttgatgt gcaccctttt ctccatcagc
     5881 agtcctctgg tccagtctaa gccctttctt agatgggacg ctcaatataa atcttttgtc
     5941 aggaatggcc gcggggagaa ctgctggcca ggccaagggt ctgacgggaa gagggggatg
     6001 ggcggcctgc gccctggacc agcttggggg acgctattaa attatgcgtt tatcagtatc
     6061 tgtgaccaat gggcgtcccg cccctcgccg ccgttgtgcg tgatggcctc gccatagcaa
     6121 taaatatcgg gcactgcact atgagtctga ccagtcatcc tggcggcaat gggctgatgc
     6181 attagatatg accaagggga aggaccgttc ggtgcctcgg gcggagcagg ggagcgcgga
     6241 gcaggtcagc ccgaccgctg accaggccga ggtggaacgc ctcagcaggt ggctggacgg
     6301 cgaggggcag ggttccgccc tgctgacctg gctcggagag ggaggggact tcgcctcaga
     6361 agatgagctg gccgagctaa aggatcgcgt agggcgctat gagatggagc ttgcggccgt
     6421 gcgcgccctt ctggcggtgg acggaaagga gggcgaggga acagcgcaac gattgcaggc
     6481 cctcattcag gaaagggatg cgctgaagga ggttgccagc aaggcggtgg cccccaacgg
     6541 gggcaacggc cttgccgaga acagggagaa ggagctcctg gaacgctacc atgccgagat
     6601 aaaggacaag gacgagcagt ggaagagggc cgagggggag ctctcggcgc gcatcgccac
     6661 cttggagaag gagctggcca agaccaagat cgagctcaag ctgaaggatg atgagcttac
     6721 ggtgctgcgc aacggaggcg gcaccgtcaa cgccgacctc cttgggaggc tggaatcgct
     6781 tcagtcaaag gagcgggagt tccttatgct caagcaggag gcggacgacc tgcgggtccg
     6841 cctcaaggag aaggaggagg agctggaccg catccgggag gccatcacct acaaggagga
     6901 cgagctcatc cgccgcgagg aggacctgat gtaccgggag aaggtctaca ccgcagagag
     6961 gaagaggctc gaggagatga aacgggagac cggagggctg gaggaggccg agctgatgaa
     7021 gaagctcgag gccctgaagg ccgaggtggc caccaaggag gatgagctcc gggccaagga
     7081 gaagtacgtc ctggccagac aggaggagct gcgtctgagg gagcagggtc tcatcgagga
     7141 cgagatcacc taccgggaga aggagcgggc gctggagctg caggagagga aggtgaagac
     7201 gggcatcccg aggttcgacg atctgctcct gggcggcatc ccgataggtt cgaacatcat
     7261 gatccatggc ccgccgttca ttggaaagga ggtcatggcc aacacattcc tcatggaggg
     7321 gctgaggaag ggcatccccg ccctgatcgt cctgacggac aggactccca aggacgtgcg
     7381 ggacgagatg aggtacatca tggccgggtt cgaggagtac gagaagctcg gtctggtgag
     7441 gtatgtggac acgtacagca gggcgatggg ggatgacgcc tccgatccgt atacggtcta
     7501 tatcgagcag ccgaacgacc atgagcggct gggagagacg atagacaagc tggccaagga
     7561 gttcaaggag aaacatgaat attataggct ggtcttccgc tcggtgtcca cgctcatcgc
     7621 ctattccgac cccaactccg ccttccgctt cctgagcccg cagtgcggac gccgcaagaa
     7681 ggaccaggcc gtgtcaatgt acatagtgga gaaggggatg catagcgagc aggagctgca
     7741 gatgctcggc tcgatcatgg acgggatggt ggacttcaag gtggaccagc tgaagacgtt
     7801 ctttgccata agagggatag gcgatgtgca gtcccgcgcc tatatccggt acacggcgac
     7861 caagagcgcc ctgaccatcg gctcgttcgc cctggaccac atccgttagg cgacctcgac
     7921 cccgttgctg ggccgcgcct ccggcctctt ctgcacattg atgcgcggca tcgggatcgg
     7981 tgcggggagc ttgatctccc gcgcgatgat aagggcggtg accatgcagt tgctgacgat
     8041 cgcgttgtga agcgcattgg cgtcgtccac cggaaggttg ttggtgcgcc tccagtcctc
     8101 catgacgctc tccccgaggt cggcgaccat gaggtgcccc tcgaccttga tgtagcccat
     8161 gccggtatag ttggcggtga aacggaagtc gatctgatgg aggccctcgt ccgccttgga
     8221 gatctgggtg atggcgctgt tgttctcgat cttgatgttc gagaccttct cgccgctctt
     8281 ggcgaaccgc ttggcctcga tggccgtgat ttcgaaggac ctgatctgca tgtcgccccc
     8341 gaatctccct ccggcttata gaacattgtc caagtcgaaa gcgtagggac cgggacaagg
     8401 acggggatgg tgccgcaggc cgggctcgaa ccagcgactt caagatatcg gcgccccggg
     8461 cgtgcgccgc cggggaactt cagtcttgca ctctcccaac tgagttactg cggctcagcc
     8521 caccccataa ttgaggacga ttaaataatt t
//
LOCUS       DAOSYN010000039         8527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_7085_length_8527_cov_3.411119, whole genome shotgun sequence.
ACCESSION   DAOSYN010000039 DAOSYN010000000
VERSION     DAOSYN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_8527_cov_3.411119"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..962
                     /locus_tag="PLJ11_02865"
     CDS             63..962
                     /locus_tag="PLJ11_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO03641.1"
                     /translation="MDAQRGAYPALAIAILAVSFASIFVRWSDSHPLVLAGYRMLFAA
                     LLLAPFALRERRKAEPLDRRTMAGVLGTGVVLAIHFFAFISSLRHTSVASSTLLVNCH
                     PLIVGAASVLLLRESSRRTVLGVAVGLIGVAALALSSVGTGEAVGNALALLGGAMAAI
                     YLLAGRVLRRRLGTLSYAFLVYMAAALVLLAAALASGVPLHPYPAEEVLLFLALAVVC
                     TIFGHTVYNWALGYLPAPVVSTALLGEPVLASMLALVLLGEAPGPAVLLAAPLVLAGV
                     LLTASDRTGKEGKDGRPEEKGPP"
     gene            983..2005
                     /locus_tag="PLJ11_02870"
     CDS             983..2005
                     /locus_tag="PLJ11_02870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO03642.1"
                     /translation="MPSGSTDGVRDLVAISVIVPVYNGEPYLRRCLDSLLAQTLEGIE
                     VIAVCDVSQDDSPGILREYQARHPNIVIIEPKANRRQGGARNMGIEAASGEYIAFVDC
                     DDWMAPAMLERLYLKAKGTGADIVDCDFVRTDGLSFEPKVSLRPECTGAMDLDKRRGA
                     MLYGGALWTKLYRRSLLIDNAVHFPEGVAYEDLLFLPLALAYARNVQKVPEPLYYYFK
                     RPGATTQARNAMHHFDRPKVAGMLVRELEERGLYAPYQEEVSWQLIVVYLETVYKALS
                     SFDRPPVDRLYELRAFMRANLPGYRTNRYYRESAQGWLRGLGALNDLSPRLLVLLYGT
                     YKKVVP"
     gene            complement(2002..5220)
                     /gene="carB"
                     /locus_tag="PLJ11_02875"
     CDS             complement(2002..5220)
                     /gene="carB"
                     /locus_tag="PLJ11_02875"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOO03643.1"
                     /translation="MSKGRLLVIGSGPIVIGQAAEFDFSGSQACRSLREEGYTTVLVN
                     SNPATIQTDLEMADVVYIEPLNVETIAEIIRKERIEGVLSGMGGQTALNLCSELAEKG
                     YLERLNCRLLGTQPRAIALSEDRELFRETMLGIGEPIPRSVTVTNIEDAKRAVEAVGG
                     FPVLIRPAYTLGGTGGGVAHNEEELVPIVGRGLIYSRIHQVLIEESVLGWKELEYEVM
                     RDRKDNCVVICTMENVDPMGIHTGESIVVAPIQTLNDEDYQRMRTASIKIIRALGIEG
                     GCNVQFAYDPSTRGYRVIEVNPRVSRSSALASKATGYPIARVAAKIAVGRTLDEIPNK
                     ITGRTFAAFEPTIDYVVTKIPRWPFDKFRTVETRIGTQMKSTGEVMAIGRTIEESFMK
                     AVRSLETDRIDLEPEIWTDQELEAELVAPSDKRLYAVAEALRRGMPVERIADLTKWDA
                     FFVHKVRSIVRMERELAEGPLTPELLWRAKRMGFPDESIAKFARRDEASVRRARIENG
                     IAPTYKMVDTCAAEFEAQTPYFYSTYGTECEVPSSGNRKVIIVGAGPIRIGQGIEFDY
                     CCVHGVMACQEAGVDAVVINNNPETVSTDYDISDRLYFEPLTLEDVLNVIEKEDADGV
                     ILQFGGQTSVNLAVPLEEALRGKRCQVLGTSPDMMDVAEDRKRWSALMRQLGIAQPES
                     GTGHSFEEVKQVADRIGYPVLLRPSYVLGGRGMEIVYSEEELRTYVATAVKVSKKHPV
                     LIDKYLTHAQELDVDLVCDGRDVFIGGVMEHIEEAGVHSGDATMVLPCQSIPPEVMER
                     VVEISNKVALALQIKGCLNLQLAVKDGTVYMLEANPRASRTVPFISKAIGVPLAKLAT
                     KVMLGKGLRELGHVGLARFDHVAVKASVFPFLKLPGVDSILGPEMKSTGEVMGIDMSY
                     GAAVYKALVSAGNRLPLEGGVYVTVSDTDKPAILPIVRELHEMGFTIYATKGTSTFLR
                     NNGIPVTTVYKVAENIAPDALGLMRRGEIKLIINTPTYSSGSRRDGYMMRRLAVELEI
                     PFLTTVQGARATVCAIKRAREGALQVRDVASYHRSAAGGPGS"
     gene            complement(5217..6293)
                     /gene="carA"
                     /locus_tag="PLJ11_02880"
     CDS             complement(5217..6293)
                     /gene="carA"
                     /locus_tag="PLJ11_02880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOO03644.1"
                     /translation="MAKCFLVLEDGTIMEGTGFGHLGDASGEVVFSTGMGGYQESLTD
                     PSFSGQILTMSYPLIGNYGVSPNDVESDRVQVSGFVVRELCDVPSPGYGGRPLGPYLE
                     EQGIPGIAGVDTRSLIIKIRSAGTMKGAIVHDDPEGALERVRRMPHPAEDNLVGKVST
                     KEVLHFPRPGAKRVAVLDCGVKLSIMEELRRRFDVYQLPYDTGVKFFRDHEIDGVFLT
                     NGPGDPSHPSLTGTIVATLRRIRDDYPMMGICMGNHLICQAFGGSTFKLKFGHRGENQ
                     PVRFRDRVFITSQNHGYAEDPATLEGSGLEIDQVNVNDGTVEGLRHRELPIFSVQYHP
                     EASPGPRDTGFLFDDFVKMLEARR"
     gene            6471..7511
                     /locus_tag="PLJ11_02885"
     CDS             6471..7511
                     /locus_tag="PLJ11_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HOO03645.1"
                     /translation="MKVLHQDRFKGEIKLQVEVADDLWHLYNILTPGDLVYASTYRRE
                     EAKSDKLRAERGEKRRMTLGIRLEKVEFGEFDDRLRLLGVIEEGPQDIGAHHTLLVEE
                     GDVLSVIKKEWRRSELERVRRAVEDAKRPCVLFISLDDDEATVAVLRQFGVQRLADIS
                     AGGHGKMYVSKEEGDYFGEIIAKAKQAYSPGVPVVVLGPGFAKETLMARGKEREPEMF
                     SRAFLYHTGQSGMTGIQELMKRGLGAEVLKGSRVAEETELVEALLERVGTDGLATYGP
                     KDVRQAAEAGAVEHLLVLDSLVREKDVESLMRAVEDARGRVTIVSELHEGGRKLEALG
                     GMGALLRFRPSA"
     gene            7747..>8527
                     /gene="aksA"
                     /locus_tag="PLJ11_02890"
     CDS             7747..>8527
                     /gene="aksA"
                     /locus_tag="PLJ11_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176519.1"
                     /note="in Methanococcus jannaschii this protein catalyzes
                     the condensation of alpha-ketoglutarate and acetyl-CoA to
                     form trans-homoaconitate; functions in alphaketosuberate
                     synthesis which is a precursor in coenzyme B and biotin
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitate hydratase"
                     /protein_id="HOO03646.1"
                     /translation="MRDRSEILAKFEKSPLITCDTTLRDGEQAAGIVFANLEKLRIAK
                     LLDEVGVQQIEAGIPAMGGDEKNAVKRIASLGLDASILGWNRASKEDVAHSIDCDVDS
                     VAISMSSSDIHIKNKLMKTREWVLEKVVESVEYAKAHGLYVSCNAEDASRADPAFLVE
                     FGRAAKEAGADRLRYCDTIGLLEPRRAYAEIKALREAVDIDIEMHTHNDFGMATANAL
                     AGVQAGARFVSTTVMGIGERTGNTPLEEIVMAAKHLLHMDVD"
BASE COUNT         1388 a         2843 c         2799 g         1497 t
ORIGIN      
        1 ggagctcctg ccgcagatcc gggcgtgcaa gggcgtgcgg tccgtggtga tccactgatc
       61 ccatggacgc ccaaagggga gcctaccccg ccctggccat cgccatcctc gccgtctcct
      121 tcgcctccat cttcgtgcgg tggagcgatt cccaccccct cgtcctcgcc gggtaccgca
      181 tgctcttcgc cgccctgctg ctggccccct tcgccctccg ggagaggagg aaggcggagc
      241 ccctggaccg gaggacgatg gccggcgtcc tcggcaccgg ggtggtgctc gccatacact
      301 tcttcgcctt catctcctcc ctccgccaca cctcggtggc ctcctccacc ctgctggtga
      361 actgccaccc cctcatcgtc ggggcggcgt ccgtgctcct tctgagagaa tcgtcccgcc
      421 ggaccgtgct cggcgtggcc gtcggcctca tcggcgtcgc cgcccttgcg ctctccagcg
      481 tgggcacggg ggaggcggtc gggaacgccc tggccctgct cggcggggcc atggccgcga
      541 tctacctgtt ggccggtagg gtgctgcgcc ggaggctggg aacgctctcc tacgccttcc
      601 tggtctacat ggccgccgcc ctcgtgctgc tggccgccgc gctcgcgagt ggggtgcccc
      661 tccatcccta tcccgccgag gaggtgctcc tattcctggc attggcggtg gtgtgcacca
      721 tcttcgggca taccgtctat aactgggcgc tagggtacct tcccgccccg gtcgtcagca
      781 cggcgctgct gggggagccc gtcctcgcct ccatgctggc cctcgtcctt ctgggcgagg
      841 cgcccggtcc tgccgtgctg ctggcggcgc ccctggtcct ggcgggggtc ctgctcaccg
      901 cgtccgacag gactggaaag gaagggaagg acgggcgccc ggaggagaaa gggcctccat
      961 agttttagta ggggcggaac gcttgccttc aggcagtacc gacggggtgc gggacttggt
     1021 ggcgatcagc gtcattgtgc cggtatataa cggggagccc tatctgcgga gatgcctcga
     1081 ctcattgctg gcccagacgc tcgagggcat cgaggtgatc gccgtctgcg atgttagcca
     1141 ggatgacagc ccgggcatcc tgagggagta ccaggcgcgc catcctaaca tcgtcatcat
     1201 agagccaaag gccaaccgga ggcagggcgg ggcgaggaac atgggcattg aggcggcctc
     1261 cggcgagtac atcgccttcg tggactgcga cgattggatg gcgcccgcca tgctcgagag
     1321 gctgtacctc aaggccaagg ggaccggggc cgatatcgtg gactgcgact tcgtgcgcac
     1381 cgacggcctt tcgttcgaac cgaaggtgag cctccgcccg gaatgcaccg gggcgatgga
     1441 ccttgacaag aggagggggg cgatgctcta cggcggggcg ctctggacca agctctaccg
     1501 gcgctcgctg ctcatcgaca acgcggtcca cttcccggag ggggtggcgt acgaggacct
     1561 gctcttcctc cccctcgcat tggcctatgc ccggaacgtg cagaaggtcc ccgagcccct
     1621 ctattactat ttcaaacgcc ccggcgccac aacccaggcc cggaacgcca tgcaccactt
     1681 cgaccgaccg aaggtggccg ggatgctggt gcgggagctc gaggaaaggg ggctgtatgc
     1741 tccataccaa gaggaggtct cctggcagct catcgtggtg tacctggaga cggtctacaa
     1801 ggccctctcc tccttcgaca ggccccccgt cgaccgcctg tacgagctgc gcgccttcat
     1861 gagggcgaac ctccccgggt accgaaccaa caggtactac agggaaagcg cgcagggatg
     1921 gctgagaggg ctgggcgcgt tgaacgacct cagtccccgc ctcctcgtgc ttctctatgg
     1981 aacgtacaag aaggtcgtcc cttaggaacc cggaccgcct gccgcgcttc ggtggtacga
     2041 ggccacgtcc ctgacctgaa gcgcgccctc ccgggcgcgc ttgatcgcgc acaccgtcgc
     2101 cctcgccccc tgcacggtgg tcaggaacgg gatctccagc tccaccgcca ggcgccgcat
     2161 catgtacccg tccctccggg agccggacga gtacgtcggc gtgttgatga tgagcttgat
     2221 ctccccgcgg cgcatcaggc ccagggcatc gggcgcgatg ttctcggcga ccttgtagac
     2281 cgtcgtcacg gggatgccgt tgttcctcag gaacgttgac gtgcccttcg tggcgtatat
     2341 ggtgaagccc atctcatgca gttccctgac gatcggcagg atggccggct tgtcggtgtc
     2401 ggagacggtg acgtacaccc ctccttccag cggcagcctg ttcccggcgg acaccagagc
     2461 cttgtagacc gcggccccgt agctcatgtc tatgcccatg acctccccgg tgctcttcat
     2521 ctccggcccc aggatcgagt ccaccccagg cagcttgagg aacgggaaga cggacgcctt
     2581 gaccgccacg tggtcgaacc gggcgaggcc gacgtgcccg agctccctga gccccttgcc
     2641 cagcatgacc ttggtggcca gcttcgccag ggggacgccg atcgccttgg atatgaacgg
     2701 cacggtgcgg gacgcccgcg ggttcgcctc cagcatgtac accgtgccgt ccttcaccgc
     2761 cagctgcagg ttcaggcagc ccttgatctg gagggcgagg gcgaccttgt tactgatctc
     2821 caccaccctc tccatcacct cgggggggat gctctggcac ggaaggacca tcgtggcgtc
     2881 ccccgagtgg acccccgcct cctcgatgtg ctccatcacg ccgccgatga agacgtccct
     2941 gccatcgcag accaggtcca cgtccagttc ctgagcgtgg gtgaggtact tgtcgatgag
     3001 caccggatgc ttcttcgaga ccttgaccgc cgtcgcgacg taggtccgca gctcctcctc
     3061 gctgtacacg atctccatgc cccgcccccc gagcacgtac gaggggcgga ggaggacggg
     3121 gtagccgatg cggtcggcca cctgcttcac ctcctcgaag gagtggccgg tgcccgactc
     3181 cggctgggcg atgcccagct gccgcatgag ggccgaccag cgcttgcggt cctcggccac
     3241 gtccatcatg tccggggagg tgccgagcac ctggcacctc ttccccctga gcgcctcctc
     3301 gagcggcacg gcgagattga ccgaggtctg ccctccgaac tgaaggatga cgccgtcagc
     3361 gtcctccttt tcgatcacgt tcagcacgtc ctccagggtg aggggctcga agtacaggcg
     3421 gtcggagatg tcgtagtccg tggacaccgt ctccgggttg ttgttgatca ccaccgcgtc
     3481 cacccccgcc tcctggcagg ccatgacccc gtgcacgcag cagtagtcga actcgatccc
     3541 ctgcccgatg cggatgggcc cggcgccgac gatgatgacc ttcctgttcc cggagctggg
     3601 gacctcgcat tcggtgccat aggtggagta gaagtagggc gtctgggcct cgaactcggc
     3661 ggcgcaggtg tccaccatct tgtacgtcgg cgctatcccg ttctcaatgc gggcccgccg
     3721 gaccgaggcc tcgtccctcc gggcgaactt ggcaatgctc tcgtccggga agcccatgcg
     3781 cttcgccctc cagaggagct cgggggtgag cgggccctcg gccagctccc gctccatccg
     3841 gacgatggag cgcaccttgt gcacgaagaa ggcgtcccat ttggtgaggt ccgctatcct
     3901 ctcgaccggc atgccgcgcc gcagcgcctc cgcgaccgcg tagaggcgct tgtccgaggg
     3961 ggcgaccagc tccgcctcca gctcctggtc ggtccagatc tccggttcca ggtcgatgcg
     4021 gtccgtctcc agggagcgga ccgccttcat gaacgactcc tcgatggtgc ggccgatcgc
     4081 catgacctcc ccggtgctct tcatctgcgt cccgatccgc gtctccaccg tccggaactt
     4141 gtcgaagggc cagcggggga tcttcgtcac cacgtagtcg atggtcggct cgaaggccgc
     4201 gaaggtcctc ccggtgatct tgttcgggat ctcgtccagg gtcctgccga ccgcgatctt
     4261 cgccgccacc cgcgctatcg ggtacccggt cgccttggag gccagggcag aggagcggga
     4321 gacgcgcggg ttcacctcga tcactcgata gcccctggtg ctggggtcgt acgcgaactg
     4381 gacgttgcac cctccctcga tgcccagcgc ccggatgatc ttgatggagg cagtgcgcat
     4441 ccgctgatag tcctcgtcat tcagcgtttg gatcggggcg accacgatgc tctcgccggt
     4501 gtggatcccc atggggtcca cgttctccat ggtgcagatg accacgcagt tgtccttgcg
     4561 gtcccgcatc acctcgtact ccagctcctt ccagccgagc acgctctcct cgatgagcac
     4621 ctggtggatg cgggagtaga tgagcccgcg gccaacgatc gggacgagtt cctcctcgtt
     4681 gtgagcgacg ccccctccgg tgccgccgag ggtgtaggcg gggcggatga ggacggggaa
     4741 cccgcccacc gcctcgaccg cccgcttcgc gtcctcgatg ttggtgacgg tgacgctgcg
     4801 cgggatcggc tcgcctatcc cgagcatggt ctcccggaac agctcccggt cctccgagag
     4861 ggcgatagcc cgaggctggg tgccgagcag gcggcagttc agccgctcca ggtacccctt
     4921 ctccgccagc tccgagcaca ggttgagggc ggtctgcccc cccatgccgg agaggacgcc
     4981 ctctatcctc tcctttcgta tgatctcggc gatggtctcc acgttcagcg gttctatgta
     5041 gacgacgtcc gccatctcca ggtccgtctg gatggtggcg gggttggagt tcaccaggac
     5101 ggtggtgtac ccttcctccc gcagggagcg gcaggcctgg gagccggaga agtcgaactc
     5161 cgccgcctgg ccgatgacga tggggccgga gccgatgacg agcagcctcc ccttgctcat
     5221 cgcctcgcct ccagcatttt gacgaagtca tcgaaaagga acccggtgtc cctgggcccc
     5281 ggactggcct ccgggtggta ctgcaccgag aatatcggca gctcacgatg gcggagcccc
     5341 tccaccgtcc cgtcgttcac gttcacctgg tcgatctcca gcccagagcc ctccagggtc
     5401 gccgggtcct cggcgtaccc gtggttctgg gaggtgatga acacccggtc cctgaagcgc
     5461 acgggctggt tctccccccg gtgcccgaac ttgagcttga aggtgctgcc gccgaacgcc
     5521 tggcagatga ggtggttgcc catacatatg cccatcatcg ggtagtcgtc cctgatcctc
     5581 ctcagggtgg cgacgatcgt gcctgtgagg gacggatggg aagggtcccc ggggccgttg
     5641 gtcaggaaca ccccgtcgat ctcgtggtcc cggaagaact tcaccccggt gtcataaggg
     5701 agctggtaca catcgaaccg ccgccggagt tcctccatta tgctgagctt gacgccgcaa
     5761 tcgagcaccg ccacccgctt cgcccccggc ctcgggaagt ggagcacctc cttggtgctg
     5821 accttcccca ccagattgtc ctcggcggga tggggcatcc ggcgcacccg ttccagcgcc
     5881 ccctccgggt cgtcatggac gatggcgccc ttcatcgtcc ccgcgctcct tatcttgata
     5941 ataagggagc gggtgtccac cccggctatg cccgggattc cctgctcctc gagatagggg
     6001 ccgaggggcc tgcccccata ccccgggctc gggacatcgc acagctcccg caccacgaac
     6061 ccggagacct ggacccggtc cgactccacg tcgttcgggc tcaccccgta attgccgatg
     6121 agaggatagg acatcgtcag gatctggccg ctgaacgacg ggtcggtcag gctctcctgg
     6181 tagccgccca tgccggtgct gaagacgacc tcccccgagg cgtcccccag atgaccaaag
     6241 ccggtgccct ccatgatagt cccgtcctcg agaaccagga agcacttcgc catcaggatt
     6301 ccacggtagc attatactgt acttaaccct tactgaaccg cccagggcgc tggaccggtc
     6361 aatgctggat gcgcgcgccc cgtccatttt caggcatacc ctctcccccc tttgtccaat
     6421 gccggacgca ttgggacaac cataaatgcc ggggagggca tctcgccccg atgaaggtac
     6481 tgcatcagga ccgcttcaag ggggagatca agctgcaggt ggaggtggcc gacgacctct
     6541 ggcacctgta caacatcctc acccccgggg acctggtgta cgcctcgacg taccgccgtg
     6601 aggaggccaa gtcggacaag ctcagggcgg agaggggcga gaagaggagg atgacgctgg
     6661 ggatccgatt ggagaaggtg gagttcgggg agttcgacga tcgcctgcgc ctcctcggcg
     6721 tcatcgagga gggcccgcag gacatcgggg cgcaccacac cctcctcgtg gaggagggtg
     6781 acgtgctctc cgtcatcaaa aaggaatgga ggcgatcgga gctggagcgc gtccggcggg
     6841 ccgtggagga cgccaagcgc ccctgcgtcc tcttcatctc cctggacgac gacgaggcga
     6901 ccgtggcggt gctccggcag ttcggggtgc agaggctggc ggacatctcc gccggcggcc
     6961 acggcaagat gtacgtttcc aaggaggagg gggactactt cggcgagatc atcgccaagg
     7021 cgaagcaggc ctattccccc ggggtcccgg tggtcgtgct tggtccgggc ttcgccaagg
     7081 agacgctcat ggccaggggg aaggagcgag agccggagat gttctcccgg gcgttcctgt
     7141 accatacggg gcagtcgggc atgacgggca tccaggagct aatgaagcgg ggcttgggcg
     7201 cggaggtgct gaagggctcc cgcgtggccg aggagacgga gctggtggag gcgctcctgg
     7261 agcgggtggg cacggacggg ctggcgacct acgggccgaa ggatgtgcgg caggcggccg
     7321 aggcgggggc ggtggagcac ctgctggtcc tcgactccct ggtcagggaa aaggatgtgg
     7381 agagcctcat gcgcgccgtc gaggacgccc gggggcgcgt tacgatcgtc agcgagctgc
     7441 acgagggcgg gaggaaatta gaggccttgg ggggtatggg ggcgctcctc cggttccgcc
     7501 catcggcatg acgccgcgcc ccggtccatg ccgcccttct ccggaacgct ttagtaaggt
     7561 cccccggacc gcggtcccgc cgcccccata tccagcctcc ggcgccgggg gctatggtgt
     7621 caacagggag acgccccttt cgcccccctg tccgattcgg gacgcctgga agaaataccc
     7681 atgtccgtat gggggaaggt cccaccggac atcaggacga tgtgcggcag agcggaaggg
     7741 aatgcgatga gagaccggtc cgagatcctt gccaagttcg agaagagccc cctgatcacc
     7801 tgcgacacca cgctccgcga cggggagcag gccgctggca ttgtcttcgc gaatttggaa
     7861 aaactgagga tagccaagct tctggacgaa gtgggagtgc agcagatcga ggctgggata
     7921 cccgcaatgg gcggggacga gaagaacgcc gtgaagagga tcgcctcctt ggggttggac
     7981 gcctccatcc tcggatggaa cagggccagc aaggaggacg tggcgcactc catagattgc
     8041 gacgttgatt cggtcgcgat ctccatgtcc tcctccgaca tccacatcaa gaacaagttg
     8101 atgaagaccc gggagtgggt gctggagaag gtggtcgaga gtgtggagta cgcgaaggcg
     8161 cacgggctgt acgtgtcctg caacgccgag gacgcctccc gcgccgaccc ggccttcctg
     8221 gtcgagttcg ggagggccgc caaggaggcc ggggcggacc gcctccgcta ctgcgacacg
     8281 atcggcctgc tggagccaag gagggcctac gccgagatca aggcgcttag ggaggcggtg
     8341 gacatcgaca tcgagatgca cacccacaac gacttcggca tggcgaccgc gaacgccctg
     8401 gcgggcgttc aggcgggggc gaggttcgtc agcaccacgg tcatgggcat cggcgagcgc
     8461 accgggaaca ccccgctgga ggagatcgtc atggccgcca agcacctcct gcacatggac
     8521 gtggact
//
LOCUS       DAOSYN010000040         8486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_7125_length_8486_cov_4.090499, whole genome shotgun sequence.
ACCESSION   DAOSYN010000040 DAOSYN010000000
VERSION     DAOSYN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7125_length_8486_cov_4.090499"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLJ11_02895"
     CDS             complement(<1..208)
                     /locus_tag="PLJ11_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO03647.1"
                     /translation="MDELDVLLSVIENPTRRKILEALVREPQYPLQLSRELGLSQQGI
                     MKHLRMLEDLDLVRSFSEESDQGGP"
     gene            422..1465
                     /gene="endA"
                     /locus_tag="PLJ11_02900"
     CDS             422..1465
                     /gene="endA"
                     /locus_tag="PLJ11_02900"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HOO03648.1"
                     /translation="MAGELVGDSVIVPDMTEGSQLYNKGYYGYPRPGGGLELELLEAL
                     YLVECAKLQVCLDGKTLDHAALYRRAARSVNGLQARYLVYRDLKQRGYIVKLDQGDFH
                     FRVYQRGSAPHNSQAKHWVLAISEREVFNIASLLEKSETSQRTRKDLLLGVVDEEGDI
                     TYYTVEGAEPHGLASEVPTKAEGALIDSSVLVLGEDADRLYENGYYGKRIGPMLQLSL
                     LETAYLMERGALEVRSLRSGQRMGEKGFLSSARRAQPDFDMRMRAYKDLRSRGMVVKT
                     GFKYGTHFRVYEGDPAKHHSRYLVHVVPDDYSTVWAEISRAVRLAHGVKKEILFASSG
                     QELSYIKLTRVRP"
     gene            complement(1462..2388)
                     /locus_tag="PLJ11_02905"
     CDS             complement(1462..2388)
                     /locus_tag="PLJ11_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO03649.1"
                     /translation="MKLKKPVLLLPSVGLIFAGLVLILSPVTAIFLFRSVNTVVGIIL
                     MLLFTVVGAWEMALSRTIAATQVGEWKGIVRTLLIALVMRLIALLLTEDLVLTLNLAA
                     MCLELLALGMVYVYRASFMPSEEEVEAALKRFATVTVKTAAQCPKCSAVVEADWSLCP
                     DCGTRLPKYCANCGTEVKETETVCCKCGTKMEVPASLLRTVETLRQTAELEAAPETRS
                     ARYARLGDGLLKIGQLDEAVEAFKAAIQYTRFPRKRTNFMVKLAVVMANKGRLQEADE
                     LLDMALKLDPEDVAGARKVLTELRGPGEGACA"
     gene            complement(2454..3386)
                     /locus_tag="PLJ11_02910"
     CDS             complement(2454..3386)
                     /locus_tag="PLJ11_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HOO03650.1"
                     /translation="MASKKEAADEVTRCPECNSGHLVRDYERGELICEECGLVIDDQF
                     IDLGPEWRAFDVEQGEKRARTGAPMTYTIHDKGLSTEISWKNKDSYGKSIPTRNRAQL
                     YRLRKWQRRIRVSNATERNLAFALSELDRMASAMGLPRNVRETAAMIYRKAVNKNLIR
                     GRSIEGVVAASLYAACRQCNVPRTLDEVASSSRVGRKEIGRTYRFMTRELSLKLMPTK
                     PQDYVQRFCSELKLSGDVQAKAADILKDANKKELTSGRGPTGVAAAAIYIASILCNER
                     RTQREVADIAGVTEVTIRNRYKELTDKLGIEIQL"
     gene            complement(3358..3621)
                     /locus_tag="PLJ11_02915"
     CDS             complement(3358..3621)
                     /locus_tag="PLJ11_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HOO03651.1"
                     /translation="MQLLGNVQEVTVDGRLVVRATFTPKLKEAVFDNRKRPLGRIVRI
                     FGPVGSPYVTVEPTGDAGLLSAVGRQVYIGGVENHGKQKGSGR"
     gene            3840..4589
                     /locus_tag="PLJ11_02920"
     CDS             3840..4589
                     /locus_tag="PLJ11_02920"
                     /inference="COORDINATES: protein motif:HMM:NF010324.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate/pantothenate synthetase"
                     /protein_id="HOO03652.1"
                     /translation="MKISKDHPRYRSLVTRERMAAMVEEGIVTPTGLIAHGRGEAFDY
                     LLGERTVPEAEAAEAAAAALLVLAERPVITVNGNTAALAAKEVGELARVVGAPIEVNL
                     FHRTDQRMERVCAFVEKETGLKVLGRDQDATLAGIASDRARCTSQGLLAADVVLIPLE
                     DGDRAEAMARAGKRVIAIDLNPLSRTALAAEVTVVDELTRAVPNITAWAKRFRGRPEE
                     ARGTLASFDNAEGLRAVRERICRGLEAPLRK"
     gene            4605..5849
                     /locus_tag="PLJ11_02925"
     CDS             4605..5849
                     /locus_tag="PLJ11_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOO03653.1"
                     /translation="MDRELLIKGSRRLGWAEAHMAVLREVGERMRQEGSLRGVRVGMA
                     LHVEAKTGMLAVTLSRAGAKVRLASCNPLSTDDSVALALREERGIEVFARKGESSEEY
                     YENLNAVLDLRPEFVIDDGADLITMLHTSRRELLGEVKGGNEETTTGVIRLKAMAAEG
                     QLRFPVMAVNDARMKYLFDNRYGTGQSTFDGFMNATNLLVAGKTVVVAGYGWCGRGVA
                     MRAKGLGAIVIVTEVDPIRAIEARLDGFQVMPMIEAVRQADVVISVTGCKDVVREEHL
                     RVMKDGCVLGNAGHFDNEVSKRALESLASRKERVREHVDRYDLMDGRRVYLVAEGRLM
                     NLAAGQGHPVEIMDMSFSIQALALEHLVRNHAAMEAKVYPVPAEMDELVARTKLRSMG
                     IAIDQLTPEQARYLCEWREGTR"
     gene            5846..6754
                     /locus_tag="PLJ11_02930"
     CDS             5846..6754
                     /locus_tag="PLJ11_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOO03654.1"
                     /translation="MRPFLCVYGHTNVDHLLALRELPERNTSANVLSKRSFFGGTGAN
                     MATMASALGVPTALVSYVGRDMPPEFRGLMEAKGVVLDELVEVEGQETPTVWIATDER
                     EDQVALIFQGAMADMASYPLRTKGAEEALAVHLMTGSPDYYLRLLSQPCLLRKRRALD
                     PAQEIHHVWDAPRFVKALGSVDMLFCNRNELATALRYTGRGGPEELLADVPVIVSTRG
                     VEGVRIFTRDETLDVPAYRVRVVDPTGAGDAFRAGFYAGLYHGKGWAECGALGCAAAS
                     FVVASPGSLTSIPDWGMVEERARALM"
     gene            6993..7286
                     /locus_tag="PLJ11_02935"
     CDS             6993..7286
                     /locus_tag="PLJ11_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03655.1"
                     /translation="MDWIGFLTVLLLTFSLVMLLAGAFTAYFGSGKSRMIGVVLLVIG
                     LIVGLLWAYLGYADELITVDLSEVIWVAFVNILAALIGALVAVGAFLLAIMKS"
     gene            7356..7823
                     /gene="purE"
                     /locus_tag="PLJ11_02940"
     CDS             7356..7823
                     /gene="purE"
                     /locus_tag="PLJ11_02940"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOO03656.1"
                     /translation="MASVLVILGSKSDAEVGRRSMDVLGRFGVEAKMVVASAHRTPDR
                     VKALVEGSGADVFIAIAGLAAALPGMVAAFTTRPVIGVPVAGKLGLDSLLSIVQMPPG
                     VPVACVGLDRGDNAALLAVAILSLADERLAEKLASYRRELAERVERDSNEVQA"
     gene            7823..>8486
                     /locus_tag="PLJ11_02945"
     CDS             7823..>8486
                     /locus_tag="PLJ11_02945"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOO03657.1"
                     /translation="MFDPRAFVEEKVNELRRTITGKAIIACSGGVDSTVAAVLVDRAI
                     GERLRAVYVDTGLMRKGETEEVRRMFDRLGVHYRIVDASGEFFAALKGQVDPERKRKA
                     IGERFIRVFEREAREFGAEYLVQGTIAPDWIESGDGVRDTIKSHHNVGGLPKDMALRL
                     VEPLWDLYKDEVRAIARFLGVEVSERQPFPGPALAIRVIGEANPESVGVVREACAIVE
                     EEL"
BASE COUNT         1477 a         2665 c         2910 g         1434 t
ORIGIN      
        1 cgggacctcc ctggtcgctc tcctccgaga aggagcggac caggtccagg tcctccagca
       61 tcctcaggtg cttcattatg ccctgctggc tgagccccag ctccctggac agctggaggg
      121 ggtactgcgg ctcccgcacg agcgcctcca gtatcttccg ccgggtgggg ttctcaatga
      181 ccgatagcag tacgtcgagc tcgtccatgt tcccacaact attcaaccat ctgtttacat
      241 ccataaattg taaactatct gtataaataa ctagcgcagg gtctgcgctg acccacgcga
      301 gcgccctcga acatcgcccc ccgggccggg cacggggtgc ccctcccgat ccctgcacgg
      361 gacagagcgc gccgcggagg ccgggcggga acatttaact gacgaacgcc cttcaagggg
      421 catggcgggt gagctggtcg gcgacagcgt catcgtcccc gacatgaccg aggggagcca
      481 gctctataac aagggctact acggctaccc aaggccgggg ggagggctcg agcttgagct
      541 gctggaggcg ctctacctgg tggagtgcgc caagctccag gtgtgcctgg acggtaaaac
      601 gctggaccac gccgccctct accggcgcgc agcccgctcg gtgaacggcc tgcaggcccg
      661 ctacctcgtg taccgggacc tgaagcagcg cggatacatc gtcaagctgg accaggggga
      721 cttccacttc cgggtgtacc agagggggtc cgcgccgcac aactcgcagg cgaagcactg
      781 ggtgctggcc atctcggagc gggaggtctt caacatcgcc tccctgctgg agaagagcga
      841 gacctcacag cgcacccgca aggacctcct gctgggggtg gtggacgagg agggggacat
      901 cacctattac acggtcgagg gggccgagcc gcatgggctt gcctcagagg tcccgaccaa
      961 ggccgagggc gccctcatcg actcctccgt cctggtgctg ggggaggacg cggaccgcct
     1021 ctacgagaac ggctactatg gaaagaggat cggccccatg ctccagctct cgttgctcga
     1081 gaccgcgtat ctcatggaga ggggcgcgct cgaggtgcgc tccctccgct ccggacagag
     1141 gatgggggag aagggcttcc tgagcagcgc acggagggcc cagcccgact tcgacatgcg
     1201 catgcgggcc tacaaggacc tgaggtcccg ggggatggtg gtcaagaccg gcttcaagta
     1261 cggcacgcac ttccgggtgt acgaggggga cccggcgaag catcactccc gctaccttgt
     1321 gcacgtggtc ccggacgact actccaccgt ctgggccgag atctcccgcg cggtcaggct
     1381 cgctcacggg gtcaagaagg agatcctctt cgccagctcc gggcaggaac ttagctacat
     1441 caagctgacc agggtgaggc cctaggcgca ggccccttcc ccggggccgc gcagctccgt
     1501 caggaccttc cgcgcgccgg cgacgtcctc agggtccagc ttgagggcca tgtccagtag
     1561 ttcgtcggcc tcctgcagcc gccccttgtt ggccatgacc accgccagct tgaccatgaa
     1621 gttcgtgcgc ttccggggga accgggtata ttggatcgcg gctttgaagg cctccaccgc
     1681 ctcgtccagt tgcccgatct tcagcagccc atcgcccagg cgggcgtagc gggccgagcg
     1741 ggtctcgggg gcggcctcaa gctcggccgt ctggcgcagc gtctccacgg tcctcaggag
     1801 cgaggcgggc acctccatct tggtgccgca cttgcagcac acggtctctg tctccttgac
     1861 ctccgttccg cagttggcgc agtacttcgg gagacgtgtg ccgcagtccg ggcagaggct
     1921 ccagtcggcc tccacgaccg cggagcactt ggggcattgg gcggcggtct tgaccgtcac
     1981 cgtggcgaag cgcttcaagg ccgcctccac ctcctcctcg ctgggcatga acgaggcgcg
     2041 gtagacgtag accatcccca aggccagcag ctccaggcac atggccgcga ggttgagagt
     2101 gaggaccagg tcctcggtga ggaggagcgc gatcaggcgc atgaccaggg cgatcagcag
     2161 ggtccgcacg atccctttcc actcccccac ctgggtggcg gcgatggtcc gggacagtgc
     2221 catctcccag gcgcccacca cggtgaagag cagcatgagg atgatgccca cgaccgtgtt
     2281 cacggacctg aagaggaata tggccgtcac gggggacagt ataaggacca ggccagcgaa
     2341 tatgaggcca acggacggta gcagtagcac gggcttcttg agcttcatcg gacacctctt
     2401 ctggcgccag cgcgcattcc attaaaatgt taagggaata agaggtttgc gggtcagagc
     2461 tgtatctcta tccccaactt gtccgtcagc tccttgtagc ggttcctgat ggtgacctcg
     2521 gtcacgccgg cgatgtccgc cacctcccgc tgggtgcgcc gctcgttgca caggatgctc
     2581 gcaatgtaga tggcggccgc cgcgaccccg gtggggcccc ggccggaggt cagctccttc
     2641 ttgttggcgt ccttcaggat gtccgcggcc ttcgcctgga cgtccccgct cagcttcagc
     2701 tcggagcaga accgctggac gtagtcctgc ggcttggtcg gcatcagctt gagggacagc
     2761 tcccgggtca tgaagcggta cgtacggccg atctccttgc ggccgacccg gctggaactc
     2821 gccacctcgt ccagcgtgcg gggcacgttg cactggcgac aggcagcgta gagggatgca
     2881 gcgaccacgc cctcgatgct gcgcccccgg atcagattct tgttgaccgc cttgcgatag
     2941 atcatcgccg cggtctcccg gacgttccgg ggcagcccca tcgccgaggc catgcggtcc
     3001 agctcactga gggcgaaggc caggttcctc tcggtggcgt tggacaccct gatccggcgc
     3061 tgccacttcc ggaggcggta cagctgggcg cggttcctgg tggggatgct cttgccatag
     3121 gagtccttgt tcttccagga gatctcggtg gaaagcccct tgtcatggat ggtgtaggtc
     3181 atcggcgcgc cggtcctcgc ccgcttctcc ccctgctcca catcaaacgc ccgccactcc
     3241 gggcccaggt ctatgaactg gtcgtcgatg accaatccgc attcctcgca gatcagctcg
     3301 ccccgctcat agtccctgac cagatgcccg ctgttgcact ccgggcatcg ggtcacctca
     3361 tctgccgctt cctttttgct tgccatggtt ctcgacccct ccgatgtaca cctgccttcc
     3421 gacagcggag agcaggccgg cgtccccggt cggttcgacg gtcacgtagg gagagcccac
     3481 cgggccgaag atccgcacga tcctgcccag cgggcgcttg cggttgtcga agaccgcctc
     3541 cttgagcttg ggggtgaagg tcgcacgtac caccagccgt ccgtccacgg tcacttcctg
     3601 cacgttccca agaagctgca tgttgagccc tatgcgccga tattaaccgg tgttgagaaa
     3661 gaggattatg aatcaactat atataggtta cgcaaagcta tataataaag cactttattg
     3721 gatataatct ttgctcaggc cgccctttct tgatgatatt taaccctatc atacccagcc
     3781 ccaaaacggc gcccggggcc gggcgcacac tttatgtcgg ggcatgccct ctaccgcccg
     3841 tgaagatatc caaggaccat cctagatatc ggtcattggt caccagggag cgcatggcgg
     3901 ccatggtcga ggagggcata gtgaccccca ccgggctcat cgcccacggc cggggggagg
     3961 ccttcgatta cctgctgggg gagaggaccg tcccggaggc ggaggccgcg gaggccgccg
     4021 ccgcggccct tctcgtgctg gcggagagac cggtcatcac cgtgaacggc aacacggccg
     4081 ccctggccgc caaggaggtc ggggagttgg ccagggtggt gggcgcaccc attgaggtca
     4141 acctgttcca ccgcaccgac cagcgcatgg agcgggtgtg cgccttcgtg gaaaaagaga
     4201 cgggactcaa ggtcctggga agggaccagg acgcgacgct cgcgggcatc gcctcggacc
     4261 gggcccggtg cacgagccag gggctgctcg ccgcggacgt cgtcctcatc ccgctggagg
     4321 acggtgaccg ggcggaggcc atggccaggg ccgggaagag ggtgatcgcc atcgacctga
     4381 accccctgtc aaggaccgcc ctggcggccg aggtgacggt ggtggacgag ctcacccgcg
     4441 cggtgcccaa catcaccgcc tgggctaaaa ggttcagggg gaggccggag gaggcccggg
     4501 gcacgctcgc ctccttcgac aacgcggagg ggctgagagc ggtgagggaa cggatctgcc
     4561 gcgggctcga ggccccgctc cggaaatgac atgagggaag ggcaatggac cgtgaactgc
     4621 tgatcaaggg atcgcgccgc ctgggatggg cggaggcgca catggcggtg ctgagggagg
     4681 tgggggagcg gatgcgccag gaaggctcat tgaggggcgt gagggtgggc atggccctcc
     4741 acgtggaggc caagacgggg atgctggcgg tcaccctttc ccgggcgggc gccaaggtcc
     4801 gccttgccag ctgcaacccc ctgtccacgg acgactcggt cgccctggcg ctcagggagg
     4861 aaaggggcat cgaggtcttc gcccgaaagg gggagagcag cgaggagtac tatgagaacc
     4921 tgaacgcggt cctggacctc aggccggagt tcgtcatcga tgacggggcg gacctgatca
     4981 caatgctgca cacctcccgc cgggagctgc tgggggaggt caagggaggg aacgaggaga
     5041 cgacgaccgg ggtcatccgc ctcaaggcca tggctgccga gggccagctg aggttcccgg
     5101 tcatggcggt caacgacgcc cggatgaagt acctcttcga caaccgctac ggcacggggc
     5161 agagcacgtt cgacggcttc atgaacgcca ccaacctact agtggccggc aagacggtcg
     5221 tggtcgcagg ctacggctgg tgcgggcggg gggtggcgat gcgggccaag gggctcggcg
     5281 ccatcgtcat cgtcaccgag gtggacccca tccgggccat cgaggccagg ctcgacggct
     5341 tccaggtcat gcccatgatc gaggccgtcc ggcaggcgga cgtggtgatc tccgtgactg
     5401 gctgcaagga cgtggtgcgg gaggagcacc tgagggtcat gaaggacggc tgcgtcctgg
     5461 ggaacgccgg tcatttcgac aacgaggtgt cgaagagggc attggagtcg ctggcctccc
     5521 gcaaggagcg cgtgagggag cacgtggacc gctatgacct catggacggg cgcagggtgt
     5581 acctcgtggc cgaaggccgg ctgatgaacc tggccgccgg gcagggccat ccggtggaga
     5641 tcatggacat gagcttctcc atacaagcgc tggcgttgga gcacctggtg cgcaaccacg
     5701 ccgccatgga ggcgaaggtc taccccgtcc cggcggagat ggacgagctg gtggcccgga
     5761 ccaagctgcg gtccatgggc atagccatcg accagctgac cccggagcag gcgaggtatc
     5821 tctgcgaatg gcgggagggc acgcggtgag gccgttcctg tgcgtctatg ggcacaccaa
     5881 cgtggaccat ctgctcgccc tcagggagct gccggagcgc aacacctccg ccaacgtcct
     5941 gagcaagcgg agcttcttcg ggggcacggg cgccaacatg gccaccatgg cctcggcctt
     6001 gggcgtgccg accgccctgg tctcctatgt cgggagggac atgccgcccg agttccgtgg
     6061 gctcatggag gcgaaggggg tggtcctgga cgaactggtc gaggtcgagg gccaggagac
     6121 ccccacggta tggatagcca cggatgagcg ggaggaccag gtcgcgctca tcttccaggg
     6181 ggccatggcc gacatggcct cctatccctt gcggaccaag ggcgccgagg aagccctggc
     6241 cgtccacctg atgaccggct cccccgacta ctacctgcgg ctgctctccc agccctgcct
     6301 gctcaggaag cgccgtgcgc tggaccccgc ccaggagatc caccacgtgt gggacgctcc
     6361 ccggttcgtc aaggccctgg ggagcgttga catgctgttc tgcaaccgca acgagctcgc
     6421 caccgccctg aggtacacag gaaggggcgg cccggaggag ttgctggccg acgtgcccgt
     6481 gatcgtcagc acccggggcg tggagggggt ccgcatcttc acgagggacg agaccctgga
     6541 cgtcccggcg tacagggtga gggtcgtgga cccgacgggg gccggggacg ccttcagggc
     6601 ggggttctac gccgggctct atcacggtaa gggctgggcg gagtgcggcg ccctgggatg
     6661 cgccgcggcc tcgttcgtgg tggcctcccc aggctccctg acgtccatcc cggactgggg
     6721 gatggtggag gaaagggcca gggccctcat gtgaccgccc cctggggtgc gaaacgaagg
     6781 cacgctcgct atctggatgc caccggctca aatgcatgaa cggagcaaaa ttataataac
     6841 tgaaataacc tctaggaggc ccaaggtgaa cgcatggcat gggaaaaggc ccctgtcctt
     6901 ccgatgtgtt cggcatccgc ggggaagggg gcgcgcccac cggtcatcgg caccaatatc
     6961 ctcgaatgaa tatcatggaa aggtgataaa gaatggattg gattggtttc ctgacggtgc
     7021 tcctgcttac cttcagcctg gtcatgttgc tggccggggc cttcacggcc tacttcggaa
     7081 gcggcaagag caggatgatc ggtgtcgtgc tgttggtcat tggcctgatc gtgggccttc
     7141 tctgggccta cctgggctac gccgatgagc tgataacagt ggacctctcc gaggtcatct
     7201 gggtcgcctt cgtcaacatc ctggcagccc tcatcggagc ccttgtggcg gtcggcgcgt
     7261 tcctgctggc catcatgaag agctgaaacc ccctcccctt tcatcgccct tccgagggca
     7321 aatccttttt tattcgaact ctgtcagtgg ccacgatggc ttcggtactc gtcattctcg
     7381 gcagcaagag cgacgcggag gtagggcgca ggtccatgga cgttctaggg cgcttcgggg
     7441 tggaggccaa gatggtggtc gcctccgccc accgcacccc ggaccgggtg aaggccctgg
     7501 tcgaagggag cggggcggac gtcttcatcg ccatcgcagg gctggcggcc gccctcccgg
     7561 ggatggtggc ggccttcacc acccggccgg tgatcggtgt cccggtggcc ggcaagctag
     7621 gcctggactc cctcctgtcc atagtgcaga tgccccccgg cgtcccggtc gcctgcgtgg
     7681 gcctggaccg cggggacaac gcggcgcttc ttgcggtggc catcctctcc cttgccgatg
     7741 aaaggctggc cgagaagctc gcctcctacc gccgggagct ggcggagagg gtcgagcgcg
     7801 attccaacga ggtgcaggcc tgatgttcga ccccagggcg ttcgtcgagg agaaggtcaa
     7861 cgagctcagg agaacgatca caggaaaggc gatcatcgcc tgctccgggg gcgtggacag
     7921 cacggtcgcc gcggtcctgg tggaccgggc catcggggag aggctgcggg cggtgtacgt
     7981 ggacaccggg ctcatgagga agggcgagac cgaggaggtc cggcgtatgt tcgaccgcct
     8041 gggggtgcac tacaggatcg tcgacgcctc gggggagttc ttcgctgcgc tgaaggggca
     8101 ggtggacccg gagaggaagc gcaaagccat cggggagcgc ttcatccggg tgttcgagcg
     8161 ggaggccagg gagttcgggg ccgagtacct ggtgcagggg accatcgccc cggattggat
     8221 agagagcggg gacggggtcc gggacaccat caagtcccat cacaacgtcg gggggctgcc
     8281 caaggacatg gccctgaggc tggtcgagcc gctctgggac ctctacaagg acgaggtgag
     8341 ggccatcgcg cgcttcctcg gcgtggaggt ctcggagcgc cagcccttcc ccggcccggc
     8401 cctggccatc agggtcatcg gggaggccaa ccccgagtcg gtgggcgtcg tccgcgaggc
     8461 gtgcgccatc gtggaggagg agctgg
//
LOCUS       DAOSYN010000041         8365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_7264_length_8365_cov_3.906017, whole genome shotgun sequence.
ACCESSION   DAOSYN010000041 DAOSYN010000000
VERSION     DAOSYN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7264_length_8365_cov_3.906017"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PLJ11_02950"
     CDS             <1..1225
                     /locus_tag="PLJ11_02950"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO03658.1"
                     /translation="TLAAGINLPARRVVVRDVNRFDDGGWVPLPVMEVKQMCGRAGRP
                     RYDPYGEAVLIARTAEQSRMLFENYLLSGPEDVESKVGNERVLRGHILALIATGTAAS
                     RAGIIAFLRRTFYCHQRGEGGLEETVEGIVRFLYEEGMVRCPGTELLVAGQDAPLSAT
                     FFGRRVSDLYIDPVSAVQLRDALLAYVPGRKELGFLHAVCSTPDMLTLYLKRDEHEPL
                     AETALQRRDELLFAVPEEGSPDHEYFLSELKTALALEAWTQEGDEEEVLRAFGIGPGD
                     LHNKVEVGEWLLYSMRELSNIFNKDCYPLLTKLMARMRYGVKEELLELVELKGVGRVR
                     ARSLYKRGYTSWERLREAPARDIAKVIGIGDALAERIKRQVDPAFVPEEGHQECAEER
                     PEGAPRQGQTSLLDF"
     gene            complement(1261..2040)
                     /locus_tag="PLJ11_02955"
     CDS             complement(1261..2040)
                     /locus_tag="PLJ11_02955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOO03659.1"
                     /translation="MPDRGPLTPAGAGGGGVPKGAAAELEKAPRAIGRMFDAIAPSYD
                     LLNHMLSMAIDVRWRKRAIRSLSLRKEDVVLDIASGTGDMALLAHAASGCAVLGLDLS
                     GNMLREAVRKCGPAYMAVQGDALRMPFPDAAFGKAMTAFGIRNMADIPAFLREARRVL
                     RPNGTLVVLEFSLPPYPLVRQAYLLYLTRVLPFVGGLRSGNRAAYQYLSDSIQRFPSP
                     RSMLALFTEAGLEVAEMASLTLGIAHLYVLRSPGAGPEGRA"
     gene            complement(2019..2915)
                     /locus_tag="PLJ11_02960"
     CDS             complement(2019..2915)
                     /locus_tag="PLJ11_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOO03660.1"
                     /translation="MALKHWAMEVRAQYLLLPVVLSLVGGALAYHDGSFDALTFGMFT
                     AVLVLLHVTVNTLNDYYDDVYGIDRAQTMRSPFNGGTGMIQAGLVSRRQVLAMAIGTA
                     VLGFMIGMYLVATVSWALLPVLLLGMGFALFYTQLFARNMLGEVSAGLGLGVLPVLGA
                     YLVQTVSLSSGAVVLASAAGLLTFNLLLLNEFPDMEADRIGGRRNLVLSLGTGRAAWL
                     YVLANGAVYGILLLFALLGEIPALSVLGLLTVPLAYRAASAAFKGPEGREAFVRGQQS
                     NVLVVLATQALVVVGICLTAAL"
     gene            complement(3131..3448)
                     /locus_tag="PLJ11_02965"
     CDS             complement(3131..3448)
                     /locus_tag="PLJ11_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HOO03661.1"
                     /translation="MSIYNEMEFDIGDHLSEDLATRNGASRFFELIESSRADMVVINF
                     EKVQSITRSFADEYIKCKRASSMTIREENVPTNIRKMLEIVSASVEKKQVVNIEELNI
                     SPL"
     gene            complement(3432..4244)
                     /locus_tag="PLJ11_02970"
     CDS             complement(3432..4244)
                     /locus_tag="PLJ11_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03662.1"
                     /translation="MAESVSYPPALVEGYLEYARIQTKYYETGTIDLGNIGWIYPSTL
                     LPCVGLVANDPDAPFIPPSDPNVTGYIAAMITRGSDNTPLGSTYVPIVALPSDERDLS
                     PVLQRLYRLNNDGREYGGECAFKYVVGEFVANIYEHSHFHHAYIMAQKYPGKGFVEFS
                     FYDDGMTIPGSLSSAGMNFKNDVEAIAMAVNGLSSKKMEGRGYGLQHNVEISIKGLMA
                     EISLVSRSGILHIDGNGPKGYMLREKYKLNGTGISVRSPYPAKEVNIYEHLQ"
     gene            complement(4871..5572)
                     /locus_tag="PLJ11_02975"
     CDS             complement(4871..5572)
                     /locus_tag="PLJ11_02975"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOO03663.1"
                     /translation="MERARVRTFVNGLDENLSGGIPKGNIIMVSGAAGTMKSSLAYYI
                     LHMNAVKEGIRGLYISLEQNKPSLMRQMEYLRMPGDPGGLVEVLDLGEIRLQAQSGGW
                     FDTFRFAVEESKKRIDYDVLVIDSLGALELIAKFEQPREEIFRLFEWLRSTEVTTFMI
                     AEMPVGQYQYYGSGDTDFLADGIIHLRMVEVGEIEVQRRLRVVKMRETDHSSSYFSFY
                     FKEGTFYVTRAITDF"
     gene            complement(5606..6835)
                     /gene="thrC"
                     /locus_tag="PLJ11_02980"
     CDS             complement(5606..6835)
                     /gene="thrC"
                     /locus_tag="PLJ11_02980"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOO03664.1"
                     /translation="MRYAVRCWECGREVEDPFLDACPACQGALTVDMDLSSLEGTSLR
                     DLRSRPLGVWRYGHFLPVQSEPVTLQEGGTPLYDCGRLADELGVREVHVKFEGANPTG
                     SFKDRGMTVGVTRALELGCRVVGCASTGNTSASLAAYAAKAGLGCVVVLPSGKVALGK
                     LAQAMFFGAKVVTVDGNFDDALRVVRELAHAGDLYMLNSVNPFRPEGQKTLAFEVLDQ
                     LDLRLPDRIIYPVGNAANIWAAYKALKEWQSLGWIDELPMLTGVQAGGSAPLVMAFNR
                     GSDNFVPVEGPETIATAIRIGNPASGRKALRAVYDTGGCMTAVSDREIIDAQLLLGRR
                     EGIGAEPASAAAVAGLRRLLDEGKVSRRERVVCVCTGHVLKDPDAVMAQCGRPIATEA
                     TAEAVRKAISQGPVPSL"
     gene            complement(6832..7773)
                     /locus_tag="PLJ11_02985"
     CDS             complement(6832..7773)
                     /locus_tag="PLJ11_02985"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOO03665.1"
                     /translation="MSPDHVIVRAPSTIANVGPGFDVFGIALAEPSDRIEVRRTDEPG
                     VRVEAITGVGAYAVTLDNTKNTAAVAAGKVLEMAEADFGLAMRIDKGIRPASGMGSSG
                     ASAAGGAFAANLLLENQLNMNQLICCASFGEEASSGSRHADNAAPALMGGFTIIRSYE
                     PMDVVRLEPPKALGIVACLPEFAVPTREARKKVPQKVPMTDVVHQVGHASALVAGMAL
                     GDLSLIARAIDDVIVEPARAPLVPFLREAERAAVDAGALASFLGGSGPCVAAFYDRDK
                     VDGLGIARAVQDFYGAHGVECFCWVTTWGTGCERVQE"
     gene            7907..>8365
                     /gene="cgi121"
                     /locus_tag="PLJ11_02990"
     CDS             7907..>8365
                     /gene="cgi121"
                     /locus_tag="PLJ11_02990"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HOO03666.1"
                     /translation="MTVRCAGARLKEGGGAPLLERASALGADVVLMDADKVCGVDHLL
                     SAAFHARRAFERGTASCNTLGMEVILYASGERQITKAKRKMGLGPGTERVALVLLDEE
                     GDMDRLIKAMGLERDDALLACTRAKGEAFGISPRELETVGEGRLQDLVLEK"
BASE COUNT         1475 a         2680 c         2750 g         1460 t
ORIGIN      
        1 gaccctggcc gcggggatca accttccggc gcggcgcgtg gtcgtgcggg acgtgaaccg
       61 tttcgacgac gggggctggg tgccgctgcc ggtgatggag gtcaagcaga tgtgcggccg
      121 cgccggccgg cccaggtacg acccctacgg ggaggcggtg ctcatcgccc gcacggcgga
      181 gcagagccgc atgctcttcg agaactacct cctgagcggg ccggaggacg tcgagtccaa
      241 ggtggggaac gaacgggtgc tgcgagggca catcctggcg ctgatcgcga cggggacggc
      301 ggcctcgcgc gccgggatca tcgccttcct ccggaggacg ttctactgcc accagcgcgg
      361 ggaggggggg ctggaggaga cggtcgaggg catcgtccgc ttcctctacg aggagggcat
      421 ggtccgctgc ccggggacgg agctcctggt ggcgggacag gacgcgccgc tgagcgccac
      481 gttcttcggg cggcgcgtct ccgacctgta catcgacccc gtgtccgccg tccagctcag
      541 ggacgcgctg ctggcgtacg tccccggcag gaaggagctg ggcttcctcc acgccgtatg
      601 ctccaccccg gacatgctga ccctctatct caagcgggac gagcacgagc ccttggccga
      661 aacggcgctc cagcggaggg acgaactcct cttcgccgtc ccggaggagg gctccccgga
      721 ccacgagtac ttcctgtcgg agctgaagac cgcgctcgcc ctggaggcct ggacgcagga
      781 gggggacgag gaggaggtgc tccgggcctt cggcatcggc ccgggggacc tgcacaacaa
      841 ggtggaggtg ggggaatggc tgctctactc catgcgggag ctgagcaaca tcttcaacaa
      901 ggactgctat cccctgctca cgaagctgat ggcccgcatg cggtacggcg tcaaagagga
      961 gctgctggag cttgtggagc tgaagggggt cggccgggtc cgcgcccgct ccctctacaa
     1021 acgcgggtac acctcctggg agaggctcag ggaggccccc gcgcgggaca tcgcgaaggt
     1081 catcggcatc ggggacgccc tggcggagag gataaagcgg caggtggacc ccgcgttcgt
     1141 tcccgaggag ggacaccagg aatgcgccga ggagaggcct gagggcgccc cgaggcaggg
     1201 gcagaccagc ctgctcgatt tttaagatgg ataccaaacg tcccagcccc tatcgggtcc
     1261 ctaggcccgg ccctctgggc ccgccccggg gcttcggagc acatagaggt gggcgatgcc
     1321 gagcgtcagg gacgccatct cggccacctc gaggccggcc tccgtgaaga gggcgagcat
     1381 cgaccggggg gaggggaacc gctggatcga atcgctcagg tactggtagg cggcgcggtt
     1441 gcccgatcgc agccccccga cgaagggcag gaccctggtc aggtacagga ggtaggcctg
     1501 ccgcaccagg ggataggggg gaagggagaa ctccaggacc accagcgtgc cattcggacg
     1561 gagcacgcgc ctggcctccc ggagaaaagc ggggatgtcg gccatgttcc ttatgccgaa
     1621 cgccgtcatg gccttgccga aggcggcgtc cgggaagggc atgcgcaggg cgtccccctg
     1681 cacggccata taggccggac cgcacttcct caccgcctcc cgcagcatgt tgccggagag
     1741 gtccaggccg aggaccgcgc acccggaggc ggcgtgggcg agcaaggcca tgtcccccgt
     1801 gcccgaggcg atgtccagga ccacgtcctc cttgcggagg gagagggagc gtattgccct
     1861 cttcctccac cgcacgtcga tggccatgga cagcatgtgg ttcagcaggt cgtagctggg
     1921 ggcgatggcg tcgaacatgc gcccgatggc ccgcggcgcc ttctccagct ccgcggccgc
     1981 gcccttcggg acgcctccgc cccccgctcc cgcaggggtc agagggccgc ggtcaggcat
     2041 atccccacga ccacgagcgc ctgggtggcg agcaccacca ggacgttgct ctgctgcccc
     2101 cggacgaacg cctcccggcc ctccgggccc ttgaaggcgg ccgaggccgc cctgtacgcc
     2161 agggggacgg tcaacaggcc gaggacggag agcgccggga tctccccgag cagggcgaag
     2221 aggaggagga tgccgtacac ggccccgttg gccaggacgt agagccaggc cgccctcccc
     2281 gtgcccaggg agaggaccag gttcctccgt ccgccgatgc ggtccgcctc catgtcgggg
     2341 aactcgttca ggaggaggag gttgaaggtg agcaggcccg ccgcgctggc caggaccacc
     2401 gcgcccgagg agagggagac ggtctgcacc aggtaggccc ccaggaccgg caggacgccg
     2461 aggccgaggc cggccgagac ctccccgagc atgttccgcg cgaagagctg cgtgtagaag
     2521 agggcgaagc ccatgcccag caggagcacc gggagcaggg cccaggacac cgtcgccacg
     2581 aggtacatcc ctatcataaa tccaaggacg gcggtgccga tggccatggc caacacctgc
     2641 cgccggctca ccagccctgc ctggatcatg ccggtgccgc cgttgaaggg gctgcgcatc
     2701 gtctgggcgc ggtcgatccc gtacacgtcg tcgtagtagt cgttcagggt gttcacggtg
     2761 acgtgcagga gcaccaggac cgccgtgaac atcccgaagg tgagcgcatc gaagctgccg
     2821 tcgtggtagg caagcgcgcc gccgacgagc gacaggacca cggggaggag gaggtactgc
     2881 gcgcggacct ccatcgccca atgcttgagg gccatcggcc ccccgaacgg gcctcctgag
     2941 aatcaacctt tcgttttccg ggcttgtgcc cagttaattg attggtccgc cctttcggtc
     3001 cgtgggagcc cttgcaggac gatgcgcatg gggcccccga tcggtccttg gtctgaatgg
     3061 cacatcctgc gggccgatcc atccttgatc tggacacagg ccgtccatgg atgcggatgg
     3121 aaaggggatg tcataggggg ctgatgttga gttcctcgat gttgacgacc tgtttcttct
     3181 ctacggatgc gctcacgatc tcgagcatct ttcggatgtt ggtcggaacg ttctcttcgc
     3241 ggatcgtcat actggacgcc ctcttgcatt tgatgtattc atcggcgaag gacctcgtga
     3301 tcgattggac cttctcgaag ttgatcacca ccatatcggc tctgctcgac tcgatgagtt
     3361 cgaagaaccg agaggcgccg ttcctagtgg ccaaatcctc ggaaaggtga tcccctatat
     3421 cgaactccat ctcattgtag atgctcatat atgttaacct ccttcgctgg gtagggcgat
     3481 cggacactaa tgccggttcc atttaattta tacttttctc taagcatata acctttcggt
     3541 ccatttccat caatgtggag aataccactc ctggaaacaa gactgatctc agccattaat
     3601 cctttaatcg agatctccac attgtgctga agaccatatc ctctcccctc catcttcttt
     3661 gacgatagcc cgttgacggc catggctatg gcctcgacat catttttgaa gttcatccct
     3721 gcggaggata ggcttccggg gatggtcatt ccatcgtcat agaaactgaa ctcgacgaag
     3781 ccttttcctg ggtatttctg ggccataata tatgcatgat ggaaatgaga atgttcgtaa
     3841 atgttggcca cgaattcgcc caccacatat ttaaatgcac attcaccacc atattctcgc
     3901 ccgtcgttat tcaatcgata cagtctttga agcacggggc ttagatccct ttcatccgag
     3961 ggaagggcga ctattggcac gtaggtgctt ccaagagggg tgttatcgga gccgcgggtg
     4021 atcatcgccg cgatataacc tgttacattg ggatcagatg gagggataaa aggagcgtcg
     4081 ggatcgttag caaccaggcc aacgcatggt agtaaggtac tggggtagat ccatccgatg
     4141 ttaccgagat cgatcgtgcc ggtttcgtag tatttggtct gaatacgcgc atattcaaga
     4201 tagccttcga ccaatgcagg cggatatgac acggattcgg ccatattgcg agggaggaaa
     4261 gaacttccat gcatattata aggtttcaac caatatcatc atggatcggg acatgaaatt
     4321 acctggtcca tcactttcaa gggattagaa aggtgggtag ttcgttgcgg cctattagga
     4381 acggatcatg atcggcaata tacccacaag aacaggggaa taggtattgg atcaatgcta
     4441 aacggaaggt cggccaatgg caggaggtgc gaggagaacc gtattcataa tattacgatc
     4501 tggatgatta tgattcgttg atatgcccac cttcaccatg ttagctggag agcgcatcgg
     4561 gaactggagt gaggcgggtc gtgacgatgg ccgacatcgt tccgggatgc tagaaggcgc
     4621 ccgcgttccc catgaagggg gcgtaaccgg ttaatccagt ggctgtgctt acctggacgg
     4681 cccccttgga ggatcgttct atggcccatt gccttgctgg aaagtcctga tcgatcgcgg
     4741 gcgatcgttt tctattcgat cctgagagcg agggaataaa agctgaggag atcctcgcca
     4801 tcaatgcttt tctctcaata ggcttccggc cttcgctatc gggaaggccc ttcgcattcc
     4861 caccgccccg tcagaagtcg gttatcgccc gggtgacgta gaacgtcccc tccttgaagt
     4921 agaaggagaa gtagctgctg ctgtggtccg tctcgcgcat cttgaccacg cggagccgcc
     4981 gctgcacctc gatctccccg acctccacca tccggaggtg gatgatgccg tccgccagga
     5041 agtccgtgtc cccggagccg tagtactggt actgccccac gggcatctcg gcgatcatga
     5101 acgtcgtcac ctccgtggag cgcagccatt cgaacagccg gaagatctcc tcgcgcggct
     5161 gctcgaactt cgcgatcagc tccagggcgc cgagggaatc gatcacaagc acgtcgtagt
     5221 cgatgcgctt cttgctctcc tccacggcga agcggaaggt gtcgaaccat ccgcccgact
     5281 gcgcctgcag ccggatctcc ccgaggtcca ggacctcgac cagcccgccg gggtccccgg
     5341 gcatgcggag gtactccatc tgccgcatca gggagggctt gttctgctcc agggagatgt
     5401 acagccccct gatgccctcc ttcaccgcgt tcatgtgcag gatgtagtac gcgagcgagg
     5461 acttcatcgt cccggcggcg ccggagacca tgatgatgtt gcccttgggg atcccgccgg
     5521 acaggttctc gtccaggccg ttgacgaacg tcctgaccct ggctctctcc atttgcctcc
     5581 cgtacgccta tggccgtccg agcatttata gtgatggcac cggcccctgg gagatggcct
     5641 tccgcaccgc ctccgccgtc gcctccgtcg cgatcggcct cccgcattgc gccataacgg
     5701 catcggggtc cttgagcacg tgcccggtgc acacgcagac gaccctttcc cgccgggaca
     5761 ccttcccctc gtccagcagc ctcctcagcc cggccacggc ggcggccgag gccggctccg
     5821 cgcctatgcc ctcgcgccgg ccgagcagga gctgggcatc gatgatctcc cggtccgaga
     5881 cggcggtcat gcagccgccc gtgtcataca ccgcccggag cgccttcctg ccggaggcgg
     5941 ggttgccgat gcggatcgcg gtggcgatcg tctccggccc ctccaccggg acgaagttgt
     6001 cgctcccccg gttgaaggcc atgaccagcg gcgccgagcc cccggcctgc acgccggtga
     6061 gcatcgggag ctcgtctatc cagcccaggg actgccattc cttgagcgcc ttgtacgccg
     6121 cccagatgtt cgccgcgttg cccaccgggt agattatcct gtccggcagc ctgaggtcca
     6181 gctggtccag gacctcgaag gccagggtct tctgcccctc ggggcggaag gggttgacgg
     6241 agttcagcat gtacaggtcc ccggcgtgcg ccagctcgcg gaccacccgg agggcgtcgt
     6301 cgaagttgcc gtcgaccgtg acgaccttgg ccccgaagaa catggcctgg gcaagtttgc
     6361 ccagcgccac cttccccgac gggaggacga ccacgcatcc cagcccggcc ttggccgcgt
     6421 aggccgccag ggaggccgag gtgttcccgg tggacgcgca cccgacgacc ctgcagccca
     6481 gctccagcgc tcgggtgacc ccgacggtca tcccgcggtc cttgaacgag ccggtcgggt
     6541 tcgccccctc gaacttgacg tgcacctcgc gcaccccgag ctcatcggcg agcctcccgc
     6601 agtcgtacag cggcgtgccg ccctcctgca gcgtgaccgg ctcgctctgg acggggagga
     6661 agtgcccgta ccgccagacc ccaaggggcc gggacctcag gtcccggagg gaggtgccct
     6721 ccagggagga gaggtccatg tccacggtca gcgcgccctg gcaggcaggg caggcgtcca
     6781 ggaaggggtc ctcgacctcg cgcccgcact cccagcaccg gacggcgtac ctcattcctg
     6841 caccctctcg caccccgtgc cccaggtggt gacccagcag aagcactcga cgccgtgggc
     6901 cccatagaag tcctgcaccg cccgcgcgat cccgaggccg tcgaccttat cgcggtcgta
     6961 gaaggcggcg acgcacggcc cggagccgcc gaggaaggag gcgagggcgc ccgcgtccac
     7021 cgccgcgcgc tccgcctccc tcaggaaggg gacgagcggg gccctcgccg gctccacgat
     7081 gacgtcgtcg atcgcccgtg cgatgaggga gaggtccccg agggccatcc cggccaccag
     7141 cgcggaggcg tgcccgacct gatgcaccac gtccgtcatc ggcaccttct gcgggacctt
     7201 cttgcgcgcc tcccgcgtcg gcacggcgaa ctccgggagg cacgccacta tgcccagggc
     7261 cttcggcggc tccaggcgca cgacgtccat cggctcgtag gagcggatga tggtgaagcc
     7321 gcccatgagc gccggggcgg cgttgtccgc gtgcctgctg ccggaggacg cctcctcgcc
     7381 gaaggaggcg cagcagataa gctggttcat gttcaattgg ttttcaagca gaaggttggc
     7441 ggcgaaggcc cccccggccg cggaggcgcc cgaggagccc atgccggagg cggggcggat
     7501 gcccttgtct atccgcatcg cgagcccgaa gtccgcttcc gccatctcca gcaccttgcc
     7561 cgccgcgacg gccgcggtgt tcttggtgtt gtccagggtg accgcgtacg ccccgacgcc
     7621 ggtgatcgcc tccacgcgga cccccggctc gtcggtcctg cggacctcga tgcggtcgct
     7681 cggctccgcg agggcgatcc cgaagacgtc gaacccgggg ccgacgttgg ctatggtgga
     7741 gggcgctcgt acgatcacgt ggtcagggga cattgtaacg ctcctttcaa aggctggtcg
     7801 acaccccatg gcctgccctc gtttaaaggt tttcagcgcc acgccctttc cgctcgatgc
     7861 gcccgccggg agaatgcttt atcccccctt cctcattgga gggggcatga cggtccgatg
     7921 cgccggggct aggctgaagg agggcggggg cgccccgctc ctcgaacggg cctcggccct
     7981 gggggcggac gtggtcctga tggacgccga caaggtgtgc ggcgtggacc atcttctctc
     8041 cgccgcgttc catgcgaggc gcgcgttcga gcgggggacc gcctcctgca acacattggg
     8101 gatggaggtc attttgtacg cctccgggga gcggcagatc accaaggcga agaggaagat
     8161 ggggctgggc ccggggacgg agcgggtggc gctcgtgctc ctggacgagg agggggacat
     8221 ggaccgattg atcaaggcca tggggctgga gcgggacgac gcgctcctcg cctgcacccg
     8281 ggcgaagggg gaggcgttcg gcatctcccc gcgggagctg gagaccgtgg gcgaggggcg
     8341 gctgcaggac ctggtcctgg agaag
//
LOCUS       DAOSYN010000042         8242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_7392_length_8242_cov_4.244534, whole genome shotgun sequence.
ACCESSION   DAOSYN010000042 DAOSYN010000000
VERSION     DAOSYN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7392_length_8242_cov_4.244534"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLJ11_02995"
     CDS             <1..435
                     /locus_tag="PLJ11_02995"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO03667.1"
                     /translation="LGFLQGDLHQFARVVASNVTMWDGTEWEGKSLFAQWGVNIIPQD
                     PYPNGPAVMLAFDTGKIDMGYLGAPPAIIKHLNVNTPESDIRIVAQANVEGSAIIVNN
                     QIQTIEDLDGKKLGTPGPASIQHLFLLAFAEEYGFDLKLAGT"
     gene            481..1305
                     /locus_tag="PLJ11_03000"
     CDS             481..1305
                     /locus_tag="PLJ11_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO03668.1"
                     /translation="MSEKDVGDQGRVRELLNKPLFKNAIATIVSLTMFILTWWLVAIW
                     ADLVYIPTPGEAFSALVDSFLHEDSNLHITMWWNIYASLKRFVLGFALALGLAIPLGL
                     VMGYSKTFEMLAKPVVEVFRPIPPIAWVPLFILIFAYFWGPVMVIFMGVLFPLLSNIY
                     FGVKSVPPILMDAARTLGATRLHIFTKVVFPYIIPFLMTGIRIGMGIGWMCIVAAEMI
                     GVEGGGLGYYISMQALTLGVYDRMFAGMIVVAVLGLLTTGLMQYLENVTNRRMGIQ"
     gene            1302..2057
                     /locus_tag="PLJ11_03005"
     CDS             1302..2057
                     /locus_tag="PLJ11_03005"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO03669.1"
                     /translation="MILEIKDLKKSFPKKKGEMVAIADFDLDVEEGEFVCLLGPSGCG
                     KTTILRIIAGLETPTSGTILLNGKQISGPGSDRGMVFQEFALFPWRTVRRNVEFGLEL
                     KGVPPEERRARTQRYIDLVGLSGFEDYHPYQLSGGMKQRVGIARALANEPSILLMDEP
                     FGALDAQTRNLMQKELLRIWSETRKTVIFVTHSVDEALFLADRIVVMTARPSTVGEIY
                     KIEWPRPRDRASPEFAALRKQILAHLESMVKAE"
     gene            complement(2058..2579)
                     /locus_tag="PLJ11_03010"
     CDS             complement(2058..2579)
                     /locus_tag="PLJ11_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOO03670.1"
                     /translation="MSMVDELVSGILRSEEGFREALRHILTEDLRISVPEFCERTGLS
                     PSTIYKILQEQREPNLRTVRAVIKGVRKLDANPGGEFIAVIASRPVLSRIEERLMKVG
                     ERTVRVKEYPACTMEDAIIAAVKAERDGALALVCAPIVAPTIEQIVRVPVSVIVPKDS
                     MVRAIEVAAAKVL"
     gene            complement(2584..>3324)
                     /locus_tag="PLJ11_03015"
     CDS             complement(2584..>3324)
                     /locus_tag="PLJ11_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO03671.1"
                     /translation="SPPRTGREMLEIAAKAAAGFGAILLEEDLLTIEMEEGGFKLTTE
                     SLREIKAKAIVLAPGVSRRKLNVEGEKEYHGLGVSYCATCDCNFFKRKTVAVLGEASM
                     AASAALLLREYASKVYWVAKEFKASPELMEKVKGTDIELVQAWPTRIYGDQVVRGMTL
                     DDGRDLPLDGVFIELGAKGVADLAMEVNIFPDENGFIAVDRSCATGTPGVFACGDVTG
                     RPWQLAKAVGEGCVAGLSAARYVRKEEE"
     assembly_gap    3325..3334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3335..3452)
                     /locus_tag="PLJ11_03020"
     CDS             complement(<3335..3452)
                     /locus_tag="PLJ11_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO03672.1"
                     /translation="MEYDVAIVGAGPAGLQAAIHAVRKKVRVCVLGRRQNSAL"
     gene            complement(3522..3926)
                     /gene="trxA"
                     /locus_tag="PLJ11_03025"
     CDS             complement(3522..3926)
                     /gene="trxA"
                     /locus_tag="PLJ11_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOO03673.1"
                     /translation="MIDDELAEIRRRKLEALMGQNELKGVNGLSGVTEVKDSTFEEFI
                     RSAPLVIIDCWAPWCGPCRMLAPIMEQLAEEYQGKIRFGKLNTDENERTPMRFNITAI
                     PTMLVFKNGMLLDRIVGAGNKDFMRQKFSKYL"
     gene            4136..4408
                     /locus_tag="PLJ11_03030"
     CDS             4136..4408
                     /locus_tag="PLJ11_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOO03674.1"
                     /translation="MLEEASELIGMQVYTPNGVFLGNVNNLVIDVDSRKVDGLFISES
                     NPLLVEDSKAVNVPFRWIQAIGDIILLKYFPKRVSARRPAPKPAQA"
     gene            4449..4946
                     /locus_tag="PLJ11_03035"
     CDS             4449..4946
                     /locus_tag="PLJ11_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOO03675.1"
                     /translation="MSGRKVYVDPAAVIIGDVELEEDVSIWPGAVLRGDANRIRVGAG
                     SNVQEGAVIHVGHDFPSAIGRMVTVGHGAVINGATVGDHCIIGINSTILDGAVIGDEC
                     IIGANALVTSMTEVPPRSVVMGLPGKVVRHNDLSIREKAERSAESYIELKDLHLEGRF
                     PRRTF"
     gene            4998..5585
                     /locus_tag="PLJ11_03040"
     CDS             4998..5585
                     /locus_tag="PLJ11_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03676.1"
                     /translation="MWKSSFRVWFYVSVATALLVSAWTTDVYWLVLELGMIGAVLGRY
                     PLFDQVEEPELQRPLTVFMVLPFTIYVLLYLLDLDGGLLLEALRTLITMMASLLLCLF
                     LTLILVKRTELHMNYRFILGFATVASISLASFFAILSTTFAFLNGEAVTNDELMWELT
                     GMMVFGIVSSLLFKRDMRRMDYVDLLVVQPKGERP"
     gene            5582..6223
                     /locus_tag="PLJ11_03045"
     CDS             5582..6223
                     /locus_tag="PLJ11_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03677.1"
                     /translation="MKVRPGTLAIMLTFEALAVILAHLAFSEGRVYPGNSALLGGLLM
                     LIPFMLEMAGLVRLPMFMQGWAFLAVGLHILGLYLGMYDNAWWWDEVTHVISSSMVGM
                     VAALGLFLFDLHSVKIKVPRWAYPLMVVTFSLFIGVVWEIAEFGGDLLAGTRMQYSLS
                     DTLHDCYVDLLGGLITSVLWVTWLWKDPEEELSKAAMPSLVRLFERTFATPQG"
     gene            6347..7648
                     /locus_tag="PLJ11_03050"
     CDS             6347..7648
                     /locus_tag="PLJ11_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOO03678.1"
                     /translation="MNVWNPHIELMSKAQLETLQLRLLKIQTRRMWEHSPFYHDRMRE
                     AGVSPDDIRTLEDVRRLPFMTKKDLRDNYPNRLMMSDRGDLVRYHASSGTTGKPIIVG
                     YTKTDLENWSESLARAMTSFGLGRKDIVQVSNTYGLFSGGLGFHYATEKIGATVVPAS
                     TGNTDRQIDLICDLGVTAMAATPSYLMYLGEVAAKRGIDMRKDTKLRVGLLGGEPWSD
                     RMRDRLYETMGVRGYNCFGASELSGPLFLECAEQNGIHIWGDIALLEIVDPDIGEPLG
                     PGERGEMVITMLQKEAFPIMRYRMGDITMMMAEPCPCGRAHPRIARIQGRVDDMIIIR
                     GINVFPSQVEHSLMQHPELGSEFQLVVDRKGHLDTMLVRAELRPEAFTDDIVALNALR
                     ERVSNRLRNVLNISVNVELVQPDTLPRFEGKAKRVMDRREY"
     gene            7648..8079
                     /locus_tag="PLJ11_03055"
     CDS             7648..8079
                     /locus_tag="PLJ11_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HOO03679.1"
                     /translation="MSDTSRYRIKQLSIFSENRPNRLGAVAKVLKEGSINILGFSIAE
                     GAGYGVIRVLVDRPDLAKDLLTEAGFVVKFTEVLAIRMEDRPGGLYDLTERMKDINIE
                     YGYGYRNPPYAVLIVRVEDIEQGIRSLLAGGLDLLDNVNFH"
BASE COUNT         1539 a         2537 c         2505 g         1651 t
ORIGIN      
        1 cttggcttcc tccaggggga cctgcaccag ttcgcccggg tggtggcgtc caacgtgacc
       61 atgtgggatg ggacggaatg ggagggcaag agcctcttcg cccaatgggg cgtcaatata
      121 atcccccagg acccctaccc caatgggccg gccgtcatgc tcgcctttga taccggcaag
      181 atagacatgg gctatctcgg ggccccgccc gctattataa agcatctgaa cgtcaacacg
      241 cccgagagcg acataagaat agtggcgcag gcaaatgtgg aggggtcggc catcatcgta
      301 aataaccaga tccagaccat cgaggacctt gacggcaaga agctgggcac ccctggcccg
      361 gcctccatcc agcacctgtt tcttctggcc ttcgccgaag aatatgggtt cgacctgaaa
      421 ctggctggta cgtgacggcg gcagcaaacc tttttatttt tttagattgt gaggagcggc
      481 atgagcgaga aggacgttgg tgatcaaggc agggtcaggg aactgcttaa caagcctctg
      541 ttcaagaacg ccatcgccac catcgtatcg ttgaccatgt tcatcctcac ctggtggctg
      601 gtggccatct gggcggacct ggtctacatc cctacccctg gcgaggcctt cagcgccctg
      661 gtggattcct tccttcatga ggactccaac ctacatatca ccatgtggtg gaacatctac
      721 gcctcgctca agcgcttcgt actgggattc gcattggccc tcggcctggc gataccgttg
      781 ggcctggtca tggggtactc caagacgttc gagatgctgg ccaaaccggt ggtggaagtg
      841 ttccggccca ttcctccgat cgcgtgggtg ccgctcttca ttctcatctt cgcctatttc
      901 tggggcccgg tcatggtcat attcatgggc gtgctcttcc cccttctttc caacatttat
      961 ttcggggtca agagcgtccc gcctatattg atggatgcgg cccgcaccct tggcgcaacc
     1021 cgcctccaca tcttcaccaa ggtggtgttc ccgtacatca tcccctttct gatgaccggc
     1081 atccgcatcg ggatggggat tggatggatg tgcatcgtcg ctgcggagat gatcggggtg
     1141 gagggcggag gccttggata ttacatctcc atgcaggccc tgaccctggg cgtctacgac
     1201 aggatgttcg cggggatgat cgtcgtggcc gtgctcggtc tattgaccac cgggctgatg
     1261 cagtaccttg agaacgtgac caaccggagg atggggatac aatgatcctg gagatcaagg
     1321 acctcaagaa gtcgttcccc aagaagaaag gggagatggt ggccatcgcc gatttcgatc
     1381 tcgacgtgga ggagggggag ttcgtctgtc tcctcggtcc gtcaggctgc ggcaagacca
     1441 ccatcctgag gatcatcgcc ggtctggaga cccccacctc cggcaccatc ctcctgaacg
     1501 gcaagcagat ctccggtccc ggctccgacc gcggaatggt cttccaggag ttcgccctgt
     1561 tcccctggcg gacggtgcgc cgcaacgtgg agttcgggct ggagctcaaa ggtgtgcccc
     1621 cggaggagcg tcgggcgcgt acccagaggt acatagacct cgtaggcctc tccggcttcg
     1681 aggactatca tccctaccag ctttcagggg ggatgaagca gcgggtgggc atcgcccgcg
     1741 ccctcgccaa cgaaccttcc atccttctca tggacgagcc cttcggggct ttggacgcgc
     1801 agacgcgcaa cctcatgcag aaggagctcc tgcgcatctg gagcgagacc aggaagacgg
     1861 tcatattcgt cacccactcc gtggacgagg ccctgttcct ggcggaccgc atcgtggtca
     1921 tgaccgcccg ccccagcacg gtcggggaaa tatacaagat cgaatggccg cgccccaggg
     1981 accgcgcctc cccggagttc gccgccctgc gcaagcagat cctggcccac ctcgagagca
     2041 tggtgaaggc ggagtgatca gaggaccttc gcggcggcca cctcgatggc ccgcaccatg
     2101 ctgtccttcg ggacaatgac ggacacaggg accctcacta tctgctcgat cgtcggcgcc
     2161 acgatcggcg cgcacaccag ggcgagggct ccgtccctct cggccttgac ggcggcgatg
     2221 atggcgtcct ccatggtaca ggcggggtat tccttgacgc ggacggtgcg ctcgccgacc
     2281 ttcatgagcc tctcctcgat gcggctcagg accggccggg aggcgatgac cgctatgaac
     2341 tccccgccgg gattggcgtc cagcttcctt acgcccttga tgaccgccct gaccgtccgc
     2401 aggttcggct cgcgctgctc ctggaggatc ttgtagatgg tgcttgggga aaggccggtc
     2461 ctttcgcaga actccgggac ggagatcctc agatcctctg tcaatatgtg ccgcagcgct
     2521 tcccggaagc cctcctcgct gcggaggatc cctgagacga gttcgtccac catgctcatc
     2581 ccatcactcc tcttcctttc tcacgtacct cgctgcggag agcccggcca cgcagccctc
     2641 cccgaccgcc tttgcgagct gccatggcct gccggtcaca tccccgcagg caaagacccc
     2701 gggcgtgccg gtggcgcagc tgcggtccac ggcgatgaac ccgttctcgt ccgggaatat
     2761 gttcacctcc atcgccaggt cggccacgcc cttggccccc agctcgatga agaccccgtc
     2821 gagcgggagg tcccgcccat cgtccagggt cattcctcgc accacctggt cgccgtagat
     2881 ccgggtaggc caggcctgga cgagctcgat gtccgtgcct ttgaccttct ccatgagctc
     2941 cggggaggcc ttgaactcct tggcgaccca atacaccttg gaggcgtact cccggaggag
     3001 gagcgcggcc gaggcggcca tgctggcctc ccccagcacc gccaccgtct tcctcttgaa
     3061 aaagttgcag tcgcaggtgg cgcagtaact gacccccagc ccgtgatatt ccttctcgcc
     3121 ctccacgttc agcttccttc gggacacccc gggggcaagg acgatcgcct tggctttgat
     3181 ctcccttagg gattccgtcg tgagcttgaa ccccccttcc tccatctcga tggtgagcag
     3241 gtcctcctcc aagaggatgg cgccgaaccc ggccgccgcc ttggcggcga tctccagcat
     3301 ctccctccca gtcctgggag gggannnnnn nnnnccaggg cgctgttctg cctccttccc
     3361 agaacgcaga ccctcacctt cttccgcacg gcatggatcg ccgcctgaag gcctgccggg
     3421 cccgccccga cgatagctac gtcgtattcc attggcatcc cctataatta gagctgcgag
     3481 agctgcgcgg tcaaacgatt tgatggaagg ggtttgcggg cctaaaggta cttggagaac
     3541 ttctgccgca tgaagtcctt gttcccggcg cccacgatgc gatccaacaa catgccattc
     3601 ttgaagacca gcatggtggg gatcgcggtg atgttgaacc gcatgggagt gcgctcgttc
     3661 tcgtccgtgt tcagcttgcc gaacctgatc ttgccctggt actcctccgc cagctgctcc
     3721 attatggggg ccagcatgcg gcagggaccg caccaggggg cccagcaatc tatgatgacc
     3781 aacggcgcgc tcctaatgaa ctcctcgaag gtgctgtcct tgacctcggt aactccagac
     3841 aatccattca ctcctttcag ttcgttctga cccatcaatg cctctagctt gcgcctcctg
     3901 atctcggcga gctcgtcgtc gatcatcaga ggaccccgtc atatcggttg tgcattgcta
     3961 ttacaaaacc gtattactgt tccatcgtat ataattttta tctctatcgt tcctagggag
     4021 cgaacctttt catcgctggt gctccgatgg aggggccgac aagggcgcgc cgcctgggaa
     4081 tgccgctgat aaccttttta ataggggatg gcaatatctg acgaagggac tgccgatgtt
     4141 agaggaggct tctgagctca ttgggatgca ggtctacacg cccaacgggg tgttcctggg
     4201 caacgtgaac aacttggtca tcgatgtgga cagccgcaaa gtggatgggc tcttcataag
     4261 cgagtccaac cctctgctcg tggaggattc caaggcggtc aatgtgccct tcagatggat
     4321 ccaggccatt ggcgacatca ttttgctcaa gtacttcccc aagagggtca gcgcccggcg
     4381 tcccgccccc aagccggcgc aggcctgagg ccaacctatt tttcttccat cccccgtata
     4441 tcattaccat gtctgggcgc aaggtctacg ttgacccggc tgcggtcatc ataggggacg
     4501 tggaactcga ggaggacgtg tccatctggc ctggggccgt cctccggggg gacgccaacc
     4561 gcatccgggt gggcgccggg agcaacgtgc aggagggggc cgtcatccac gtcggccatg
     4621 acttcccgag cgccatcggc aggatggtga ccgttgggca tggggctgtg ataaacgggg
     4681 cgaccgtcgg ggaccattgc atcatcggga taaactcgac cattttggac ggggcggtga
     4741 taggggacga atgcatcatc ggggccaatg cgctcgtcac cagcatgacc gaggtcccgc
     4801 cgcgctcggt ggttatgggg ttgcctggta aagtcgtcag gcacaacgac ctgagcatac
     4861 gggagaaggc ggagcgcagc gccgagagct acatagagct caaggacctc cacctggagg
     4921 gacggttccc ccgccggacc ttctaggccc caggcttgga attgataata aaggagcagg
     4981 gcgttcaccg gaaggacatg tggaagagca gtttccgggt gtggttctac gtgagcgtcg
     5041 ccactgccct gctggtgagc gcttggacca ccgacgtcta ttggctcgtc ctggagctgg
     5101 ggatgatcgg ggccgtcctg ggaaggtatc ccctgttcga ccaggtggag gagcctgagc
     5161 tccagcgccc cctgaccgtg ttcatggtcc tgcccttcac catatacgtc ctcctctacc
     5221 tcctggacct ggacggcggc ctattgctgg aggcgctcag aacgctcatc accatgatgg
     5281 catccctcct gctgtgcctc ttcctcaccc tgatcctggt gaagaggacg gagctccaca
     5341 tgaactacag gttcatactg ggcttcgcca cggtcgcctc catatcattg gcgtcgttct
     5401 tcgccatcct ctccacgacc ttcgccttcc tgaatgggga ggcggtcact aacgacgaac
     5461 tcatgtggga attgaccggg atgatggtct tcggcatcgt ctccagcctt ttgttcaagc
     5521 gggacatgcg ccggatggac tacgtcgacc tgctcgtcgt gcagcccaag ggggaaaggc
     5581 catgaaggtg aggccgggca ccctggcgat catgctgacc ttcgaggccc ttgcagtcat
     5641 cctggcccat ctggcctttt ccgaggggag ggtatatccc gggaacagcg cgctcctggg
     5701 aggcctcctc atgctcatcc ccttcatgct ggagatggca ggcctggtcc gactgccgat
     5761 gttcatgcag ggctgggcct tcctggccgt ggggctgcac atcctcgggc tgtacctggg
     5821 aatgtacgac aacgcgtggt ggtgggacga ggtcacccat gtgatctcct cctcgatggt
     5881 cggcatggtc gccgccctgg ggctcttcct gttcgacctg cactccgtca agatcaaggt
     5941 gccccgctgg gcctatcccc ttatggtcgt aacgttctcg ttgttcatcg gggttgtctg
     6001 ggagatagcc gagttcggag gggacctcct cgccggcacg cgcatgcagt attcgctgag
     6061 cgacacgttg catgactgct acgtcgacct gctgggcgga ctgatcacca gcgtcctttg
     6121 ggtgacgtgg ctgtggaagg accccgagga ggagctctcc aaggcggcca tgccgtccct
     6181 ggtgcggctg ttcgagcgca cgttcgcgac acctcagggg taagaggcac ccattcgacg
     6241 cagggcctgg agagcattta tatgatgcgt gttatcaccc tacacacttc accggaccgc
     6301 cgggcgcccc gtacgcatcg ccggaggccc ccctggagga aggcaattga acgtttggaa
     6361 cccgcacatc gaactcatgt ccaaagccca gctggaaacc cttcagctga ggctgcttaa
     6421 gatccagacc aggcgcatgt gggagcactc gcccttctac catgaccgga tgagagaggc
     6481 gggggtctcc ccggacgaca tcaggacgtt ggaggatgtg cggaggctgc ccttcatgac
     6541 caagaaggac cttcgggaca actaccccaa tagacttatg atgtccgacc gcggggacct
     6601 ggtccgctac cacgcctcct ccgggaccac ggggaagccc atcatcgtcg gatacacgaa
     6661 gaccgacctg gagaactggt ccgaatcgtt ggccagggcc atgacctcct ttggtctcgg
     6721 caggaaggac atcgtccagg tgagcaatac ctacggcctg ttctcgggag ggctggggtt
     6781 ccactacgcc accgagaaga tcggggcgac ggtcgtgccc gcctccaccg ggaacacgga
     6841 caggcagatc gacctgatct gcgacctcgg ggtgacggcc atggcggcca ccccctccta
     6901 cctcatgtac ctgggggagg tggcggccaa gagggggatc gacatgcgga aggacacgaa
     6961 gctccgggtc ggcctcctcg gcggggaacc ttggagcgac cggatgcggg accgcctcta
     7021 cgagacgatg ggggtccgcg gctacaactg cttcggggcg agcgagctgt ccggaccgct
     7081 cttcctggaa tgcgcggagc agaacggcat ccatatctgg ggggacatcg cgctgctcga
     7141 gatcgtggac ccggacatcg gggagccgct gggcccgggg gagagggggg agatggtgat
     7201 caccatgctg cagaaggagg ccttccccat aatgcgctac aggatgggcg acatcaccat
     7261 gatgatggcc gaaccctgtc cctgcggtcg ggcgcatccg cgcatcgccc gcatccaggg
     7321 acgcgtggac gatatgatca tcatccgtgg catcaatgtg ttcccctcgc aggtggagca
     7381 ctcgctgatg cagcatcccg agcttggcag cgagttccaa ctggtggtgg accgcaaagg
     7441 gcatctggac accatgctgg tgcgcgctga gctgaggccg gaggccttca cggacgatat
     7501 tgtcgcgctg aacgccctgc gcgagcgcgt ctccaaccgg ctgaggaacg tgctcaacat
     7561 ctcggtcaac gtggagcttg tgcagcctga cacgttgccc cggtttgagg gcaaggccaa
     7621 gcgggtaatg gacaggaggg aatactgatg agcgatacca gtaggtacag gatcaagcag
     7681 ctttccatct tctcggagaa ccggcccaat cgcctcgggg cggtggcgaa ggtgctcaag
     7741 gaagggagca tcaatatcct tggcttcagc atcgccgagg gcgccgggta cggcgttatc
     7801 cgggtgctgg tcgatcggcc cgacctcgca aaggacctcc ttacagaggc agggttcgtg
     7861 gtcaagttca ccgaggtgct ggcgatccgg atggaggatc gaccaggggg cctctatgac
     7921 ctcacggagc ggatgaagga catcaacatc gagtatggct atggatatag gaaccctccc
     7981 tatgccgtcc tgatcgtccg cgtggaggac atcgaacagg gcataaggtc cctgctggcg
     8041 ggaggcctgg acctgcttga caacgtgaac ttccattgat gcgcaccaaa ggagctgaga
     8101 aatccccatg gtctattgga accccataga ggtcatgccc atccccgagc ttaaggccct
     8161 tcagctcaag cggttgaaga tgctggtata caagctgtac accttctccc ccctatatcg
     8221 gcaaaggatg agggaggcgg gg
//
LOCUS       DAOSYN010000043         7877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_7860_length_7877_cov_4.282792, whole genome shotgun sequence.
ACCESSION   DAOSYN010000043 DAOSYN010000000
VERSION     DAOSYN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7860_length_7877_cov_4.282792"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLJ11_03060"
     CDS             <1..489
                     /locus_tag="PLJ11_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03680.1"
                     /translation="ANVKFKYLNPTDLAQAMATKLIDAMVGSEPWAINTENLCGASVH
                     ELGNSSGLGSTFPIVIVASEKALKEKGEALERFLAALDRANEYILSDWDDAMAICASH
                     TGLSVEDQSKGSGLQFFELGFNETDVQSIAMTAMYLLDLEKIEAVSVIMDHVDLRFLP
                     GE"
     gene            494..1549
                     /locus_tag="PLJ11_03065"
     CDS             494..1549
                     /locus_tag="PLJ11_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO03681.1"
                     /translation="MRGRERFLTALRRERPDGPPVFLRDLTLALDQTGYTTPEVCAGR
                     YDPDKAASSIMALHHRLGQDAVVGCVHFLGMEVEALGGEVSYPTWGIPSVARPPFQGT
                     NDPALVPLDLKRKEPFPNVLECYRRVAEGLRGEAAIVCNLEGPLTKAALLRGLENLAL
                     DMHYDPALVQRLVDYATALGAEYLEAVAAEAPIDCAFIAAASDNPDLFGREAFVRYSV
                     PHVKRLRERATGIGLPTVFHPHGDMASVANLPLMEEVIASGVEGFQFAEGNDAGALKR
                     RFGERICLMGGIDAFSTLLLGPEGRIAEEAEGYLRAFRPWGGYVFMCSCSLHRGMPLE
                     NVDALMRCVRRFPREGV"
     gene            complement(1654..1941)
                     /locus_tag="PLJ11_03070"
     CDS             complement(1654..1941)
                     /locus_tag="PLJ11_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03682.1"
                     /translation="MSGNRLIWFHGRECPHCRKLAPLVDQFEAETGIRLERLEVWHND
                     ENAKLMRSLAAVIAPACGGDLGVPAFYNEATGKAICGGRIDMPKLLAWAKG"
     gene            complement(1938..2369)
                     /locus_tag="PLJ11_03075"
     CDS             complement(1938..2369)
                     /locus_tag="PLJ11_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOO03683.1"
                     /translation="MKRLWRCHVCNDVHWGNAPPEVCPTCGATNSFVPSDLGEALEII
                     GRDHPALDDREGVLAAWQWFSDQSPTVRLTAKADEMELLCKGVLENLRTKGQRYCPCR
                     LPTGDREKDEGLICPCNFLRQPTFKETGECWCGLFVRRGTQ"
     gene            complement(2433..2939)
                     /locus_tag="PLJ11_03080"
     CDS             complement(2433..2939)
                     /locus_tag="PLJ11_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO03684.1"
                     /translation="MKRDPVLSRRSVRRYTAEDVDDEDVRKLLEAAMAAPSAGNERPW
                     HFVVVRDKETLQRLMEVHPHANALKECAGAILVCGDPSLERYPGFWPQDCSAATENIL
                     IMAVELGLGAVWLGTYPIEERVDGMRRALGIPATIVPFALVPFGHPAEETKPIDRFDA
                     TRVHEERW"
     gene            3023..3640
                     /locus_tag="PLJ11_03085"
     CDS             3023..3640
                     /locus_tag="PLJ11_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOO03685.1"
                     /translation="MPGFDELIYATTLLFFIFDPFATVPMFITLTKDQSEKEQMASAN
                     KAIIVASALFVVFALIGPQLLALFSITVDAFRIAGGIVLLLMAMEIIFSLKLSKKEET
                     SVAWVIIATPMLTGPGVITTAILLVQLYGHFTVIIAGVISLSITWGIMRNSLLISQRI
                     GNNAIEVFSKIIGMLLAAIAVEFIMRGAVDYVLNSINAAFLLPLL"
     gene            3840..4571
                     /locus_tag="PLJ11_03090"
     CDS             3840..4571
                     /locus_tag="PLJ11_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03686.1"
                     /translation="MTKCAICNSETASPKTIPVRMTAITAGEDLRMETNEAHLRALDM
                     AVHLLGHINAFRALSEVPDYLGRRRARYAMTLIMLDELTSLMELMKECERAVTERSPE
                     VLVKGFFSPEHKQERAQRALDEIGKSEALFLKVKRTLGREPSAIDFALFKDRLRQMDG
                     NVFSTVERALRYDWSLGGDSLPSDEEIDMLFMAIELNVRGAAEFAYQWAMANEFDIDF
                     RLATVNSKDGCDGRKERGYWVTMPL"
     gene            4671..4832
                     /locus_tag="PLJ11_03095"
     CDS             4671..4832
                     /locus_tag="PLJ11_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03687.1"
                     /translation="MRKEVGPEVDRAEREFGLDMLGMRVDADRGHLLAFRYKVPARPP
                     SSSSATGRG"
     gene            4981..6231
                     /gene="hisD"
                     /locus_tag="PLJ11_03100"
     CDS             4981..6231
                     /gene="hisD"
                     /locus_tag="PLJ11_03100"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOO03688.1"
                     /translation="MWQALSVESWKARGRTDLGKVFPAVSDIIEQVRREGDRAVLELT
                     ARFDKVALKDLRVSSEEVHEAYSAVDPELLEALRFAKGRIEAYHLRQRPEQVTLFKEE
                     GSVLGWKYSPLASVGIYIPGGRASYPSTALMCAVPAKVAGVRSIIACTPPPVHPLTLV
                     ALDMAGVGNIFKCGGAQAIAAMGLGTATVPKVQKVVGPGNVYVTAAKVLLRQEVEMDF
                     PAGPSELAVLADGSADPQNVAADILAQAEHGPDSACVLVTCDPILAETVGKTIEAMAE
                     GSERVDHIRSSLRNSGYILVRSLQEGVEVCNQLAPEHLSVQVRDPLSLLPCIESAGAI
                     FLGHDSPVACGDYTTGTDHVLPTAGNASVHSGLDVLHFMKRSSVQMLDRETLKRLAPA
                     TMRLAEAEGLHAHADSVRVRLGER"
     gene            complement(6236..6646)
                     /locus_tag="PLJ11_03105"
     CDS             complement(6236..6646)
                     /locus_tag="PLJ11_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03689.1"
                     /translation="MVAIPDEVLKVLNDDASVRVIATKSKEGDVHAIQVGSLKAPSPD
                     TIIVGAILMKRTGKNLESMKERGELVSILAGNQKASYEIRVKVKDKVTSGPMYDQMNA
                     SLQKMGLKANAVWVFEVKEVWNQTAGYSAGTKMV"
     gene            complement(6770..7573)
                     /locus_tag="PLJ11_03110"
     CDS             complement(6770..7573)
                     /locus_tag="PLJ11_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOO03690.1"
                     /translation="MFGIPALIEYTELRPNLNLCRELGMDFLELNMNLPYSFPENISS
                     PEIRGAMREGLRFSMHLYDELDLGSLQPSVREGHMRRCREALRWGSRHGVFLLNLHMS
                     PGVYFTLPDGKEWVYERYLEEHIGALNASLSELSALAEPLGMKICLENTGHFALPFMR
                     RAAEAVLDLPSVGLCWDIGHDARSGQREEAFMLAHLDRVWHMHFHDWDGRSDHQLPFT
                     GRLDCDRYMRIALENAMSVLIEVKTEGAIRAAVRTMRERGYLPAPRSEQ"
     gene            complement(7620..>7877)
                     /locus_tag="PLJ11_03115"
     CDS             complement(7620..>7877)
                     /locus_tag="PLJ11_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03691.1"
                     /translation="RVLAPQGLLYVSVFGEEDMRCGKGKEMEELTYLRGNGIACHYFL
                     EGEVPGLFPTLTLERHWERRLDKRYHGRPEVRQERRYLFRG"
BASE COUNT         1424 a         2503 c         2500 g         1450 t
ORIGIN      
        1 gccaacgtca aattcaaata tctcaacccc accgatctgg cacaggctat ggccacgaag
       61 ctgatcgatg ccatggtagg gagcgagccc tgggccatca acaccgagaa cctctgcggc
      121 gctagcgtcc atgagctcgg caactcctca ggcctcggaa gcaccttccc cattgtcatc
      181 gtggcctcgg agaaggcctt gaaggaaaaa ggagaggcct tggaacggtt cctcgcagcc
      241 ctcgaccggg cgaacgagta catactgagc gattgggacg acgccatggc gatctgcgct
      301 tcgcataccg gactgtccgt agaggaccaa tccaagggaa gcgggctgca gttcttcgaa
      361 ctggggttca acgagacgga cgtacagagc atcgccatga ccgcgatgta cctgctcgat
      421 ctcgagaaga tcgaggccgt ttcggtcatc atggaccacg tggacctccg cttcctgcct
      481 ggggagtgat cgagtgcggg ggagagagcg attcctcacg gccctgcgca gggaaaggcc
      541 ggatggcccg ccggtgttcc tgagggacct caccctcgcc ctggaccaga ccgggtacac
      601 gaccccggag gtgtgcgccg gccgctacga tccggacaag gcggcctcct cgatcatggc
      661 gctgcaccat cggctggggc aggacgccgt ggtgggatgc gttcacttcc tgggtatgga
      721 ggtcgaggcc ctgggcgggg aagtgtcgta cccgacctgg gggatcccca gcgtggcgcg
      781 gccccccttc cagggcacga acgacccggc cctcgttcct ctggacttga aaaggaaaga
      841 gccgttcccg aacgtgctgg aatgctacag gcgggtggcc gaggggctga ggggggaggc
      901 ggcgatagtg tgcaacctgg aaggcccgct gacgaaggcc gcgctcctgc gagggttgga
      961 gaacctcgct cttgacatgc actacgatcc cgctcttgtc caaaggctcg tcgactacgc
     1021 aacggcactc ggggcggagt acctcgaggc ggtggccgcg gaggcaccga tcgattgcgc
     1081 cttcatagcc gccgcctccg acaatccgga cctgttcggg cgggaggcgt tcgtccgata
     1141 ttcggtcccc catgtcaagc gcctgcggga aagggccaca gggatcggcc tcccaacggt
     1201 gttccatccc cacggggaca tggcctcggt cgcgaacctg ccgctgatgg aggaggtcat
     1261 cgcctcgggg gtggagggct tccagttcgc ggaggggaac gacgccgggg cgctgaagcg
     1321 aaggttcggg gagaggatat gcctgatggg gggcatcgat gccttctcca cgctgctgct
     1381 tggaccggag ggacggatag cggaggaggc ggaggggtac ctccgggcgt tccgcccctg
     1441 gggcggctat gtgttcatgt gctcctgctc cctgcaccgc ggcatgcccc tggagaacgt
     1501 ggacgccctg atgcgatgcg tgcggcggtt ccccagggaa ggggtatagg tcttcgatcg
     1561 gtcccactcc ccctcgtctt gatccaaagg tgcgcccgga cattataggc cgaaaggctc
     1621 ctttcaaagg acgcccctag ggcccgaaag cccttatccc ttcgcccagg ccaggagctt
     1681 cggcatgtcg atgcgcccgc cgcatattgc cttcccggtc gcctcgttgt agaacgccgg
     1741 aacccccagg tccccgccgc acgcaggggc gatgacggcg gcaagggaac gcatcaattt
     1801 ggcattctcg tcgttgtgcc agacctccag tctctccagc ctgatccccg tctcggcctc
     1861 gaactgatct accagcggcg ccagcttcct gcagtgaggg cattcccttc catggaacca
     1921 gatcaacctg ttgccgctca ttgcgtcccc ctccggacga agagcccgca ccagcactcc
     1981 cccgtctcct tgaacgtcgg ctgcctgagg aagttgcagg ggcatatcag cccctcatcc
     2041 ttctcccggt ccccggtggg gaggcgacag gggcagtagc gctgcccctt cgtcctcagg
     2101 ttctctagga cccccttgca gagaagctcc atctcatcgg ccttggccgt aaggcgcacg
     2161 gtcgggctct ggtcggagaa ccactgccag gccgccagga cgccctcgcg gtcgtccagg
     2221 gcggggtggt ccctcccgat gatctccagc gcctcgccga ggtccgaggg cacgaaggag
     2281 ttcgttgcgc cgcaggtcgg gcagacctcc ggaggggcgt tgccccaatg cacatcgttg
     2341 cacacgtggc atctccacag ccgcttcatt ccatctccga gaccgatagg caggcacgaa
     2401 aatataatgg ctgaggtctt cccttgcgag ggtcaccatc gctcctcgtg gaccctggtg
     2461 gcatcgaagc gatcgatcgg cttcgtctcc tcggccggat gtccgaaggg caccagggcg
     2521 aacggcacga tagtcgccgg gatccccaat gccctgcgca tcccatcgac ccgttcctcg
     2581 atgggatagg tccccaacca gactgccccg aggcccagct ccacggccat tatgaggatg
     2641 ttctcggtgg cggccgagca gtcctgcggc cagaagccgg ggtacctctc cagggaagga
     2701 tcgccgcata ccagtatcgc ccctgcgcac tccttgagtg cattggcatg ggggtgcacc
     2761 tccatcaggc gctgcaaagt ttccttgtcc cgaacgacca cgaaatgcca ggggcgctcg
     2821 ttcccggcgg agggagcggc cattgcggcc tccagcagct tccgtacgtc ctcatcgtcc
     2881 acgtcctcgg cggtataccg ccggacgctc ctccttgaaa ggaccgggtc ccttttcatc
     2941 gagggaacta agagggcgcg cgcacaaaaa cctatcggga tggtgcgaaa aatggatatc
     3001 gggcatcgag ctatcttcac ctatgccagg gttcgacgag ctgatctacg ccaccacgct
     3061 gctgttcttc atcttcgacc ccttcgccac cgtccccatg ttcatcaccc tcaccaagga
     3121 ccagagcgag aaggagcaga tggcgagcgc caataaggcg atcatcgtgg cgagcgccct
     3181 gttcgtcgtc ttcgccctga taggccccca gctgctggcg ctcttcagca ttaccgtgga
     3241 cgccttccgc atcgcggggg ggatcgtcct cctcctgatg gcaatggaga tcatcttctc
     3301 cctgaagctg agcaagaagg aggagaccag cgtggcgtgg gtgataatcg cgacccccat
     3361 gctcaccggt ccaggggtga taaccacagc catcctgctc gtgcagctct acgggcactt
     3421 cacggtcatc atcgccgggg tgatcagcct ctccatcacc tggggcataa tgcggaactc
     3481 gctcctcatc tcccagcgga tcggcaacaa cgccatcgag gttttctcca agatcatcgg
     3541 catgctcctt gcggccatcg cggtggagtt catcatgcgg ggggcggtgg actatgtgct
     3601 gaatagcatc aacgcggcct tcctgcttcc gctgctctga tgcgtccgtg caaagcattt
     3661 tatctagcct gcacgaacga ggttcggaag gcccgagggg gccatcggcc ttgcgttggc
     3721 atcggcaaag gggggagggg ccgctcctcc gctggggccg aaggcatggc cgcgccttgg
     3781 aggccgctac cggcggtccg gccaaatgct gccgtccaga ggaaaggaca cgtgatcata
     3841 tgaccaagtg cgcgatctgc aatagtgaaa ctgcctcgcc caagaccatc ccggtcagga
     3901 tgaccgccat caccgccggg gaggacctgc ggatggagac caacgaggcg cacctccggg
     3961 cgctcgacat ggccgtgcac ctcttgggcc acatcaacgc cttccgggcg ctgtccgagg
     4021 tgccggacta tctgggaagg cggagggccc gctacgccat gacgctgatc atgctcgacg
     4081 agctcacctc cctcatggag ctcatgaagg aatgcgagag ggccgttacc gagcggtccc
     4141 cggaggtgct cgtcaagggg ttcttctccc ctgagcacaa gcaggaaagg gcgcagcggg
     4201 cgctcgatga gatcggcaag tcggaggcgc tcttcctcaa ggtgaagcgg accctgggac
     4261 gggagccttc ggcgatcgac ttcgcgctct tcaaggacag gctccggcag atggacggga
     4321 atgtgttctc cacggtggag cgggcattgc gctacgactg gagcttagga ggggatagcc
     4381 tgccctcgga cgaggagatc gacatgctct tcatggccat agagctgaac gtgcgggggg
     4441 cggccgagtt cgcttatcaa tgggcgatgg ccaacgagtt cgacatcgat ttcaggctgg
     4501 ccaccgtcaa tagcaaggac gggtgcgacg ggcgcaagga aaggggctat tgggtcacca
     4561 tgcctttgtg agcctaaggc cataagagcc gtcggcggcg cgctcttcct cgctctgctc
     4621 cggaggaaga ggcgcctcat agttctggag ctctggtccc aggctgccct gtgcgcaagg
     4681 aggtcgggcc ggaggtggac agggccgaac gggagttcgg cctggacatg ctgggcatga
     4741 gggtcgatgc ggaccgcggc catcttctgg ccttcaggta caaggtgccg gcgcgcccgc
     4801 cttcatcttc ctctgcgacg ggaagaggat agcgggatac gtcaacgccg cggtcctccg
     4861 gaacatggcc gttgacaagt tgcggggcca tgcgctttgt ccggtcgata ggggatgggc
     4921 cacgggatgg acggctgcgg ctgaacatca ttatctaata cagcggccat cacccccccc
     4981 atgtggcaag cgctgtccgt cgagtcatgg aaggcccgtg gccgcacgga cctgggcaag
     5041 gtgttccctg cggtaagtga catcatagag caggtgcgga gggaggggga cagggcggtg
     5101 ctcgagctca ccgcccgctt cgataaggta gcgctcaaag acctccgggt atcgagcgag
     5161 gaggtccatg aggcgtactc ggcggtggac ccggagctgc tggaggcctt gcggttcgcg
     5221 aaggggagga tcgaggccta tcacctccgg cagcggccgg agcaggtcac cctcttcaag
     5281 gaggagggga gcgtcctggg ctggaagtac tccccccttg cgtccgtggg gatctacatc
     5341 ccggggggca gggcttccta tccaagcacg gccctcatgt gcgccgtgcc ggccaaggtc
     5401 gcgggggtcc ggagcatcat cgcctgcacc cccccgccgg tgcacccgct cacgcttgtg
     5461 gcgctggaca tggctggggt gggcaatata ttcaagtgcg ggggggcgca ggccatcgcg
     5521 gccatggggc tggggacggc gaccgtgccc aaggtgcaga aggtcgtcgg cccggggaac
     5581 gtgtacgtga ccgcggccaa ggtcctgctg cggcaggagg tggagatgga cttcccggcg
     5641 ggcccgagcg aactcgcggt ccttgccgac ggatcggccg atccgcagaa cgtcgccgcg
     5701 gacatcctgg cgcaggcgga gcacggcccg gactcggcct gcgtcctcgt gacctgcgat
     5761 cccatcctcg cggagaccgt cggaaaaaca atcgaggcga tggcggaggg gagcgagcgc
     5821 gtggaccata tccgctcgtc cctgaggaac agcggctaca tcctcgtgcg gtccctgcag
     5881 gaaggcgtgg aggtgtgcaa tcaactcgcc ccggagcacc tgtccgttca ggtgagggac
     5941 ccgctctccc tccttccgtg catcgaaagc gcgggcgcga tcttcctcgg tcacgattcc
     6001 ccggtggcct gcggggacta caccaccggc acggaccatg tgctcccgac ggcgggcaac
     6061 gcctcggtgc actccggcct ggacgtgctg cacttcatga agcggtcctc ggtgcagatg
     6121 ctggacaggg aaacgctgaa aaggctggcc ccggccacca tgcggttggc cgaggcggag
     6181 ggtttgcacg cgcacgccga ctcggtgcgc gtccggctcg gggaacgata gccgctcaga
     6241 ccatcttggt gccggcgctg tacccggcgg tctggttcca gacctctttg acctcgaaca
     6301 cccagaccgc gttggccttc aaccccatct tctgcaggga ggcgttcatc tggtcgtaca
     6361 tcgggccgga ggtcaccttg tccttgacct tgacccggat ctcgtaggag gccttctggt
     6421 tcccggcgag gatggagacc agctcccccc tctccttcat gctctccagg ttcttcccgg
     6481 tccgcttcat caggatggct ccgacgatga tcgtgtccgg ggagggggcc ttgaggctcc
     6541 ctacctggat ggcgtgcacg tccccttcct tcgacttcgt agctatgacc ctgaccgatg
     6601 catcgtcgtt caatactttc agtacttcat ccggtatcgc taccatgttt tcacccttat
     6661 atgcatgtga tcgaggcatt ataaatcatt gcaacagaaa aatgaccatg ggatatctgg
     6721 gcatgcttca aaggccagca ttctcattgg actgatatga ccatttgggt cattgctctg
     6781 acctcggagc gggcaggtac ccccgctccc gcatagtcct cacggcggcc cggattgccc
     6841 cctcggtctt gacctcgatg aggacggaca tggcgttctc gagggcgatg cgcatgtagc
     6901 gatcacagtc cagccttcca gtgaagggga gctggtgatc gctcctccca tcccagtcgt
     6961 ggaagtgcat atgccagaca cggtccaggt gggcgagcat gaaggcctcc tccctttgac
     7021 cggagcgggc atcgtgcccg atgtcccagc acagtccgac cgagggcagg tccagtacgg
     7081 cctcggccgc gcgacgcatg aacggcagcg cgaaatgccc cgtgttctca aggcatatct
     7141 tcatacctaa gggctccgcc agcgccgaaa gctccgatag ggatgcgttg agcgcgccga
     7201 tgtgctcctc caggtatctc tcgtacaccc attcctttcc gtccgggagg gtgaaataga
     7261 ccccggggct catatggagg ttcaaaagga acacgccgtg gcgggagccc cagcgcagtg
     7321 cctccctgca cctcctcata tgcccctcgc gcaccgaggg ctgtagggag ccgaggtcca
     7381 attcgtcata cagatgcatg ctgaagcgca gcccctcccg catcgcccct ctgatctccg
     7441 gggaactgat gttctccggg aaggaatagg ggaggttcat gttcagctcg aggaagtcca
     7501 tgcccagctc cctgcagagg ttcagattgg gccgtagctc cgtgtattct ataagtgccg
     7561 ggatgccgaa catgcgccct cgcccctcga gacggcgtat cgtagataaa ggattgggct
     7621 catcccctga aaaggtaccg tcgctcctgc ctgacctccg gcctcccatg atagcgcttg
     7681 tcgagcctcc tctcccaatg cctttcgagc gtcagcgttg ggaagaggcc tggtacctcc
     7741 ccttcaagaa aataatggca cgcgatcccg ttcccccgca gataggtcag ctcctccatc
     7801 tccttccctt tcccgcaccg catgtcctcc tcgccgaaca cgctcacgta taaaagcccc
     7861 tgcggggcca ggacccg
//
LOCUS       DAOSYN010000044         7874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_7866_length_7874_cov_4.235068, whole genome shotgun sequence.
ACCESSION   DAOSYN010000044 DAOSYN010000000
VERSION     DAOSYN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7866_length_7874_cov_4.235068"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PLJ11_03120"
     CDS             complement(<1..1401)
                     /locus_tag="PLJ11_03120"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOO03692.1"
                     /translation="MKSNIEIAQEAKALPIERVAAQIGIAEEELIPYGRYMAKVPLPV
                     LRRYEGCPDGRLVLVTAITPTPAGEGKTVTTIGLVEALGKQGRRVMGAIREPSLGPTF
                     GLKGGATGGGLAQVYPMWDIDLHFTGDIHAVGAAHNLLSAILENHIAKGNRLNIDPTR
                     IFLRKAIDMNCRELRNIVVGLGGRKEGGIPHESGFIITVASEISAILALTTSMTDLKE
                     RLGEILVGYTYDNRPVHAKELGCVGAMATLLKDAIHPNLVQTLEGQPFFIHGFPFANI
                     AHGNSSIIATKYALKLADVVVTEAGFGADLGAEKFFDIVCRSNGLRPNCAVLVCSIKA
                     LKMHGGCPLEAVGCEDLETLRAGLPNLDKHIDNLRHFGVPIVVAINHFAADTEAEIAL
                     VREHCREMGVRSALSDVFARGGEGGLELADQVLEAMEKDPCAFRFLYDVEMSLKDKIS
                     RIATEMYGAKRVEYETA"
     gene            1502..2407
                     /locus_tag="PLJ11_03125"
     CDS             1502..2407
                     /locus_tag="PLJ11_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOO03693.1"
                     /translation="MGARILEGKQVADGVKRGLRERIAVLEARGVVPGLAVIMVGDDP
                     ASASYVSMKGKACQELGLRSRALRMPVDLPEDELLKLIDGLNHDPEINGILVQMPVPK
                     HIDVGKVIAAIDPDKDVDAFHPINVGKMLIGSGDGFLPATPHGIQVLLAAYGIDVAGK
                     HVVIIGRSNIVGKPLAAILMQKGKGADATVTVCHSRTRDLRAITTQADVLVCAVGKAG
                     FVTADMVREGAVVVDVGSNRIEDPAAPRGYRFVGDVDFEAVREKASAITPVPKGVGPM
                     TIAMLMYNTVLAAERALERQGGPKV"
     gene            2437..3096
                     /locus_tag="PLJ11_03130"
     CDS             2437..3096
                     /locus_tag="PLJ11_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HOO03694.1"
                     /translation="MLRECGEHGYFRGDACPVCGEPGKFLMSDQELDQLGRTMAGVLR
                     HFPERFGLDMDEQGFVSIREFIAALRESNRRYHWLRPHHIIAIIETDPKGRYQVSNDL
                     MRATYGHSLDLELRLPTDNIPDRLYYPATPEEADIILETGLRPSDRKMVHLSKTYEDA
                     MNAGRVRVESPIILKIDAAAAIRAGNVIAQAGRTVYLAHEIPPEFLTRSDEEHDDLEA
                     P"
     gene            complement(3213..3482)
                     /locus_tag="PLJ11_03135"
     CDS             complement(3213..3482)
                     /locus_tag="PLJ11_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03695.1"
                     /translation="MSVISVSAITTAATSSAVSITTAIGIGAGGAIIAILLIMLLSSR
                     ELISASSKGSKKVLATLDSAIVPLLVVFSLTVVFQVLEIVGTIGG"
     gene            3619..4512
                     /locus_tag="PLJ11_03140"
     CDS             3619..4512
                     /locus_tag="PLJ11_03140"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HOO03696.1"
                     /translation="MFETADELMRRKGAMAFERHALSWTWEGFRPSLVTYDVKRREVQ
                     GLSAPFQPGQALDLTVSEARRCVGSFGDAGYAPCMLRQVVSGQFDQCPICASTWIPVQ
                     ACIFEPTCDGVHTECKGGSICAREHHVYAAFYGDLIKVGMTLSSRFMERAVEQGADAI
                     VKLGSYPNRLEARKAENALSKALHATQWVRRTSFLRAQGARLDREAYQERLDRLLSAL
                     AGVRVEPEAVQPLNEYPLAPFDPASVSFAEVAGRHRGEVLGCKGKWLFYRPRASEVLM
                     LEMARVPSRFITLNLGNHNIS"
     gene            4556..5509
                     /locus_tag="PLJ11_03145"
     CDS             4556..5509
                     /locus_tag="PLJ11_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1743 domain-containing protein"
                     /protein_id="HOO03697.1"
                     /translation="MVQVLKPEEVTDRYGRMFCRGVYTLVDERNGVAQVREECSARGP
                     VEWDACNRKRAGGAMTKVTVEGNTIIMDTIIGEGEVRFGPASKDLGGQGLRSLIVDGD
                     KVRTTWVGLAGASVGIGACLPQGPGTISAEYPDEVHIGGAHQVEVTIVTPKLVRVIIA
                     VDDTDTKQQGASWSMVLKVARECPVGTLLEHKIVQLNPNVPEKTTNCVSVGVSFAVRE
                     KDLPALLAYFKEALRKNTFSQETTMAYFVGLRIPKELEEYGWRAKSVIYNIGQAMDVA
                     SRNGVEVVEITGRRGTIGAVAAIGCFDLGVRAAGLPEDFES"
     gene            5538..6320
                     /gene="uppS"
                     /locus_tag="PLJ11_03150"
     CDS             5538..6320
                     /gene="uppS"
                     /locus_tag="PLJ11_03150"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOO03698.1"
                     /translation="MSNEITRIIANTAYQTYEKRLFKEVMEGAIPEHVAIIMDGNRRF
                     AQEIGLAVHEGHVKGKDKLEEVMEWCQEMGIRVLTVFAFSTENLQRDEGEVQCLMDLF
                     EESFLRLAEDERIHENRIRVTVLGQRELLPERVIRAIEVAEGRTRDYDQYFYNIAIAY
                     GSRQEMIMAIKRIAAKVKEGELAVDDIDEKVVSSFLYTADFKDPDLVLRTSGEERVSN
                     FLLWQLAYSELYFTDVYWPGFRKVDFLRAIRTYQARQRRFGK"
     gene            6373..>7874
                     /locus_tag="PLJ11_03155"
     CDS             6373..>7874
                     /locus_tag="PLJ11_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04190 family B12-binding domain/radical SAM
                     domain protein"
                     /protein_id="HOO03699.1"
                     /translation="MRKEDIIFLHAPSVYDFRKEFLFYGPVSDMVPSTPVFEMYPFGF
                     TTMAAHLAKGGHRVRVVNLASMMLNDRRLDVERLISRLRADVFGIDLHWLPHAHGALE
                     VAKLVKRYHPEAKVLMGGFSATYYHEELLGYPQVDMVLKGDSTEEPLLQLMQALRDGT
                     PLERVPNLSWKEGGTHHHNPITHVPDTLDDQDIDYGWIVRSVIRHMDLEGHKPFKDWD
                     RYPITMALSIKGCHLNCTVCGGSCQAMRGFLNRQRPAFRSPEKLAQDVFNIQRYIKGP
                     TFIVGDPRRGGVDYMERFFKEVKRLGVTNSLIFEFFTPPDEEFYRLASANTEGFAVEI
                     SPDSHDPEVREALGRRYSNEELEGSIRNALRYGAERVDVFFMIGLPKQTRESAVRSAE
                     YARHLYLLADKDPRLCVFISPLAPFLDPGSMAFEDPERYGYVSFARTLEEHRARLLAP
                     SWKHVLSYETKWMTRDDIAEVSYEAADVINRARFECGLIDKEELEFRLRRS"
BASE COUNT         1474 a         2404 c         2613 g         1383 t
ORIGIN      
        1 cgcggtctcg tactccacgc gcttggcccc gtacatctcg gtggcgatcc tgctgatctt
       61 gtccttcagg gacatctcca catcgtacag gaagcggaag gcgcaggggt ccttctccat
      121 ggcctcgagg acctggtcag cgagctcgag ccccccctcc ccgcccctgg cgaagacgtc
      181 ggagagggcg gagcgcaccc ccatctcccg gcagtgctcc cgcaccaggg cgatctccgc
      241 ctcggtgtcg gcggcgaagt ggtttatggc gacgacgatg ggcaccccga agtgccggag
      301 gttatcgatg tgcttgtcca ggttgggaag gccggcgcgg agcgtctcga ggtcctcgca
      361 tccgaccgcc tccagggggc agccgccgtg catcttaagg gccttgatgg agcagaccag
      421 gactgcgcag ttcggccgga ggccgttgct cctgcatacg atgtcgaaga acttctctgc
      481 gccgaggtcg gccccgaagc cggcctcggt caccaccacg tccgccagct tcagcgcgta
      541 cttggtggcg atgatggagc tgttgccgtg cgcgatgttg gcgaacggga agccgtggat
      601 gaagaagggc tgtccctcca gggtctggac caggttgggg tggatggcgt ccttcagaag
      661 ggtggccatg gcccccacgc accccagctc cttggcgtgg acggggcggt tgtcgtacgt
      721 gtagccgacc aggatctcgc ccaggcgctc cttgaggtcg gtcatggagg ttgtgagcgc
      781 caggatggcg ctgatctccg aggccaccgt gattatgaac ccgctctcgt gcgggatccc
      841 tccctccttc cgccccccga ggcctaccac tatgttgcgc agctcgcggc agttcatgtc
      901 gatcgccttc ctcaggaaga tgcgggtggg gtctatgttc agcctgttcc ccttggcgat
      961 gtggttctcg aggatcgctg acaggaggtt gtgcgccgcc cccacggcgt ggatgtcccc
     1021 cgtgaaatga agatcgatgt cccacatggg atagacctgg gcgaggccgc cgcccgtcgc
     1081 cccgcccttg agcccgaagg tcggtccgag ggaaggctcg cggatggccc ccatcaccct
     1141 ccggccctgc ttccccagcg cctcgaccaa cccgatggtc gtcaccgtct tcccctcccc
     1201 ggccggcgtg ggggttatcg cagtgaccaa caccagcctt ccgtccggac agccctcgta
     1261 ccgcctgagc accggcaggg gcaccttggc catgtagcgg ccgtagggga tcagctcctc
     1321 ctctgcgatc ccgatttggg cggccaccct ttcgatcgga agcgccttgg cctcctgggc
     1381 tatctcgatg ttgctcttca tgcactccct tcgggaccga tatccagacg cgccttaaat
     1441 ggtttgccgg gggccggcaa ttctttaata cggctaaggc atcaagagcc ccgggccgga
     1501 catgggcgct aggatcttgg agggcaagca ggtcgcggac ggggtcaagc ggggcctgag
     1561 ggaaaggata gcagtgcttg aggcgcgggg cgtggtcccc gggcttgccg tcatcatggt
     1621 gggcgacgat cccgcctcgg cctcctacgt gagcatgaag ggcaaggcct gccaggagct
     1681 ggggctgcgc tccagggcgc tccgcatgcc ggtcgacctc ccggaggatg aactgcttaa
     1741 actgatcgac ggcctgaacc atgacccgga gatcaacggc attctggtgc agatgcccgt
     1801 gcccaagcac atagacgtgg gcaaggtcat agcggccatc gatccggata aggacgtgga
     1861 cgccttccat ccgatcaacg tcggtaagat gctcatcggc agcggggacg gcttccttcc
     1921 cgccaccccg catggtatcc aggtcctgct cgctgcgtac ggcatcgacg tcgccgggaa
     1981 gcatgtggtg ataataggca ggagcaacat agtggggaag cccctggcgg ccatcctcat
     2041 gcagaagggg aagggcgccg acgccacggt caccgtctgc cactcccgga ccagggacct
     2101 gagggcgatc accacgcagg cggacgtgct ggtctgcgcc gtcgggaagg cgggcttcgt
     2161 gaccgcggac atggtcaggg agggggcggt cgtggtggac gtcggctcca acaggatcga
     2221 ggacccggcc gcgcccagag gctaccgctt cgtgggggac gtcgacttcg aggccgtgcg
     2281 ggagaaggcc tcggcgatca cccccgtgcc caagggggtg gggccgatga ccatcgccat
     2341 gctcatgtac aatacggtgc tggctgcgga gagggccttg gagcgccaag gggggccgaa
     2401 agtttaagtt accccttgcc cgttcgaggg gacgttatgc tgagggaatg tggtgagcac
     2461 gggtactttc gaggggacgc ctgccccgtc tgcggggagc cgggcaagtt cctgatgagc
     2521 gaccaggagc tggaccagct gggccgcacc atggcagggg tcctccgaca cttcccggag
     2581 cgcttcgggc tcgacatgga cgagcagggc ttcgtcagca tccgggagtt catagccgcg
     2641 ctccgggaga gcaaccgccg ctaccactgg ttaaggccgc atcacatcat cgcgatcatc
     2701 gagaccgatc ccaagggcag gtaccaggta agcaacgacc tgatgagggc cacctacggc
     2761 cactcgctgg acctggagct aaggctgccc accgacaata tcccggaccg tctttactac
     2821 cccgcgaccc cggaggaggc ggacatcatc ctcgagaccg gtctcaggcc ctcggaccgc
     2881 aagatggtcc acctctcgaa gacgtacgag gacgccatga acgccggtag ggtgagggtg
     2941 gagtccccga taatcctgaa gatcgacgcg gcggcggcca tcagggctgg gaacgtcatc
     3001 gcgcaagcgg gaaggaccgt atacctggcg catgagatcc cgccagagtt cctgacccgt
     3061 tcggatgagg agcatgacga tctagaggcg ccttgagcga agggcgtcgc cctcaacgcc
     3121 gatatcctct atcctttaag ggacccttca caggaaagga tcttcgagga ccaatgaaaa
     3181 gatggaaaag gatgacgggg cgttgggcga actcaaccac ctatcgtgcc cactatctcc
     3241 aggacctgga acacaacggt cagggagaag accacgagca gtgggactat ggccgagtcc
     3301 aatgtggcca gcactttctt tgagcctttg ctggaagcgg agatcagctc ccgggaggag
     3361 aggagcatga tgagcaggat ggcgatgatc gccccgcccg cccctatccc gatagcagtg
     3421 gtgatggata cggcgctgct ggtggccgcc gtagtaattg cactgacaga tatcacggac
     3481 atttaactta ctttacgggc tatgtgatat aaatggtttt ttcaagcggt ctcacttttg
     3541 atactgtgtt tggaccgctt cctcatgatc ggcggaccgt gccacacgta taaatcggcg
     3601 ttcctcgatg ttattcctat gttcgagacc gccgatgagc tcatgcggcg gaagggggcg
     3661 atggcctttg agcggcacgc cctctcctgg acgtgggagg ggttccggcc gtccctggtg
     3721 acgtatgacg tcaagcggag ggaggtccag gggctctccg ctcccttcca gccaggccag
     3781 gcgctcgacc ttaccgtctc cgaggccagg cgctgcgtgg gctccttcgg cgacgcaggg
     3841 tacgcgccct gcatgctgcg ccaggtggtc tccggccagt tcgaccagtg cccgatctgc
     3901 gcctccacct ggatccctgt gcaggcctgc atcttcgagc ccacctgcga cggggtgcac
     3961 actgagtgca aggggggtag catctgcgcc cgggagcatc acgtatatgc ggcgttctac
     4021 ggggacctga tcaaggtggg gatgaccctc tcctcccgct tcatggaaag ggcggtggag
     4081 cagggcgcgg acgccatagt caagctcggc agctacccca accggctgga ggccagaaag
     4141 gccgagaacg ccctttcgaa ggccttgcac gccacgcaat gggtccggag gacctccttc
     4201 ctgcgggccc agggcgcgag gctggaccgg gaggcgtacc aggagaggct cgaccgcctc
     4261 ctctccgcct tagctggggt gcgggtcgag ccggaggcgg tgcagcccct gaatgagtat
     4321 cctctcgctc ccttcgaccc tgccagcgtt tccttcgccg aggtggccgg gaggcatcgc
     4381 ggcgaagtgc tgggatgcaa gggcaagtgg ctcttctacc gtccgcgcgc cagcgaggta
     4441 ctgatgctgg agatggcccg ggtcccgtcg cgcttcatca ccctgaacct tggaaaccat
     4501 aatatctcgt gacgatatcc ttccttctcg ttcaatcggt ccaagggatg tcagaatggt
     4561 gcaagtgctc aagccggagg aggtcacgga cagatacgga aggatgttct gccgtggcgt
     4621 gtacacgttg gtggacgaga ggaacggggt ggcccaggtc agggaggagt gctcggccag
     4681 gggcccggta gaatgggacg cctgcaaccg caagcgcgcg ggaggggcga tgaccaaggt
     4741 caccgtggag ggcaacacca tcatcatgga cactatcatt ggggagggcg aggtgcgctt
     4801 cggtcccgcc tcgaaggacc tgggggggca ggggttgcgc tccctcatcg tcgacgggga
     4861 caaggtgcgc accacgtggg tgggccttgc gggggccagc gtcggcatcg gcgcctgcct
     4921 cccccagggc ccgggcacca tctccgctga gtacccggat gaggtccaca tcggtggcgc
     4981 ccaccaggtg gaggtcacca tcgtaacgcc caagctggtg cgcgtgatca tcgccgtgga
     5041 cgacacggac acgaagcagc agggggcctc gtggtccatg gtgctgaagg tcgcccggga
     5101 gtgccccgtc gggacgctcc tcgagcacaa gatcgtgcag ctgaacccaa acgtgccgga
     5161 gaagactacc aactgcgtct ccgtgggggt cagcttcgcg gtcagggaga aggaccttcc
     5221 agccctgctc gcctatttca aggaggccct gcggaagaac accttctccc aggagaccac
     5281 catggcctat ttcgtcggcc tgcggatccc gaaggaactc gaggagtatg gatggagggc
     5341 gaagagcgtc atctataaca tcgggcaggc gatggacgtg gcctcccgga acggggtgga
     5401 ggtggtggag atcactggcc gccggggcac catcggggcg gtggcggcca tagggtgctt
     5461 cgacctgggc gtgagggcgg cgggcctgcc cgaggacttc gaaagttaga ttaccaccga
     5521 gcggattggt aacctcgatg tccaatgaga tcacccgcat tatcgcgaac accgcctatc
     5581 agacctacga gaagcggctg ttcaaggagg tgatggaagg cgccatcccg gagcacgtgg
     5641 cgatcatcat ggacggcaac cgccgcttcg cccaggagat cggactggca gtgcatgagg
     5701 ggcatgtcaa gggcaaggat aagctcgagg aggtgatgga gtggtgccag gagatgggga
     5761 tcagggtcct caccgtcttt gccttctcaa ccgagaacct ccagcgggac gagggcgagg
     5821 tccaatgcct gatggacctg ttcgaggaga gcttcctgag gctggccgag gatgagcgca
     5881 tccatgagaa ccgcatcagg gtcaccgtgc tcgggcagcg ggagctgcta ccggagaggg
     5941 tgatcagggc catcgaggtc gccgagggca ggaccaggga ctatgaccag tacttctaca
     6001 atatagccat cgcctatggc agccggcagg agatgataat ggccatcaag cgcatcgccg
     6061 ccaaggtcaa ggagggggag ctggccgtgg acgatatcga cgagaaggtg gtgtccagtt
     6121 tcctctacac cgcggacttc aaggaccccg atctcgtgct gcgcacctcc ggggaggaac
     6181 gggtctccaa cttcctcctc tggcagctcg cgtactccga gctctacttc accgatgtgt
     6241 actggcccgg gttccgcaag gtggacttcc tccgggccat tcgcacctat caggccagac
     6301 agcggcgctt cgggaaatga ctgaggcgca gtttaaatag ctccgtgcgc gtcagcgtgc
     6361 cagaggacag gtatgcgcaa agaggatatc atattcctgc acgcccccag tgtctacgac
     6421 ttccggaagg agttcctatt ctatgggcca gtgagcgaca tggtcccgtc caccccggtc
     6481 ttcgagatgt accccttcgg cttcaccacc atggctgccc atctagcgaa gggcggccac
     6541 agggtcaggg tcgtgaacct ggcgagcatg atgctcaacg accgccggct ggacgtcgag
     6601 cggctgatct ccaggctgag ggcggacgtc ttcggcatcg atctgcactg gctcccccat
     6661 gcccacgggg cgctggaggt ggccaagcta gtcaagcgct atcacccgga ggccaaggtc
     6721 ctcatgggcg ggttctcggc gacctactac cacgaggaac tgctgggata tccccaggtg
     6781 gacatggtcc tgaagggcga ctccaccgag gagccgctcc tgcagctcat gcaggccctg
     6841 cgggacggca cgccgttgga gcgggtccct aacctgagct ggaaggaagg cggcacgcac
     6901 catcacaatc ccatcaccca cgtgcccgac accctggacg accaggacat cgactacgga
     6961 tggatcgtgc gctccgtgat ccgccacatg gacctggagg gacacaagcc gttcaaggac
     7021 tgggacagat acccgataac gatggccctc tccatcaagg gctgccatct gaactgcacg
     7081 gtctgcggag gctcatgcca ggcaatgcgc gggttcctga acaggcagcg cccggccttc
     7141 cggtccccgg agaagctggc ccaggatgtg ttcaacatcc aacggtacat caagggcccc
     7201 acgttcatcg tcggggaccc gcgccgggga ggcgtggact atatggaaag attcttcaaa
     7261 gaggtgaagc ggctgggcgt gaccaattcc ctcatctttg agttcttcac ccccccggac
     7321 gaggagttct accggctggc gagcgccaac accgaggggt tcgcggtgga gatctccccg
     7381 gactcccacg acccggaggt gcgcgaggcc ctcggccgga ggtattcgaa cgaggagctg
     7441 gaggggtcga taaggaacgc gctaaggtac ggcgcggagc gggtcgacgt cttcttcatg
     7501 ataggcctac ccaagcagac cagggagtcg gcggtgcgga gcgccgagta cgccaggcac
     7561 ctctaccttc tggcggacaa ggacccccgg ctgtgcgtgt tcatctcccc gctcgccccg
     7621 ttccttgacc cgggctcgat ggcctttgag gacccggaga gatacggata cgtctctttc
     7681 gcccgcacgc tggaggagca ccgcgcccgc ctgctggcgc cctcgtggaa gcatgtgctg
     7741 tcctacgaga ccaaatggat gaccagggat gacatcgccg aggtgagcta cgaggcggcc
     7801 gacgtcatca acagggcgcg cttcgagtgc ggcctcatag acaaggagga gttggagttc
     7861 cgtttgaggc gctc
//
LOCUS       DAOSYN010000045         7851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_7901_length_7851_cov_4.178297, whole genome shotgun sequence.
ACCESSION   DAOSYN010000045 DAOSYN010000000
VERSION     DAOSYN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7901_length_7851_cov_4.178297"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3587
                     /locus_tag="PLJ11_03160"
     CDS             <1..3587
                     /locus_tag="PLJ11_03160"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO03700.1"
                     /translation="LRRRWCRARGAVAFIGNSRYGWYARGSTYGSSQRFDKAFFSQVY
                     DGDAPELGRALAYSKESLYTSALSAGTMRWVYLELNLLGDPETSVHLSERATHDLEVR
                     GIEVGRCIAGERCQVTVEVRNVGQSMDEGALRLLVDGETLGNAPVSLAPGEGMTVMMG
                     WTPSGAGRHELVAVVACEGDESPENDRSDLEVMVDRRVTSDEHWSGEVVLSSGLLIAE
                     GVTVTARDCYILLRSSPMLYRLTVDGAFLMDNCTYGGSPSVLDSAGGELAMTECLMEG
                     ASLSLAQGARFGSLSLRNTSMVGGSGWQIEGANVVMTNSSAVAQEGEWLISDSTVAID
                     LVDGAGGHGLHLVNASGTVSRLSWTGGSSGLIVERCSGMTLHDLSLVGNAMDMDIEGD
                     VTAHFIHDLRNVTLTAGPLAVLHGLDGTTLTGAFASLYLVECHAVVVRGQRFQGAGTG
                     LALIESHGIEVIGNAIENCTTGILAIGSQGTVWANDLLDNDAQVHQARSDLTFDRGYP
                     RGGNHWSDMRGLDQMSGDGQDAMGCDGIFDSPYEGGAYDRYPKVGRCSYLYDSPNASF
                     IINAVPANRVDPVVLTHCGGSGIGIANWTWDLGDGNMAYGDRAVHTYLSLGTAPVLLT
                     VTDHKGSTATATGEVLVVNLLPDCGFIVSPSRPAPGEAVRFEDRSSDRDGSISAWRWD
                     FGDGGTSLEPEPEHTYQEEGEYLVSLTVMDAEGGSASTTQALAVGNDPPVATFVSAPS
                     LITVLTDVRFTSQSHDPDGEVVSWAWSFGDGATGTGSSVVHRYSSLGTFQVTLEVWDE
                     HGASSSVTASLTVRNSRPVADFEAPGSVLSLEEVHFVDLSYDLDGRVLGWSWDFGDGG
                     TSTLASPAHIYKTPGDYAVTLTVMDDRRASSVKMAPVKVLNRLPQVAIEGPSGEHWSL
                     EEMEFHAMASDMDGTVASFHWDMGDGRELEGQTVCHAYLCPGEYVVTLTCEDDAGGVA
                     NATIVVQVQNLIPLVGATYERSLDHPLELMFQSGAEDLDGSIASYDWRFGDGGSSAEA
                     APAHRYRRAGDYTVVLNVTDDRGAWAVAFANVTVVEGSLFLTGMTCEKADGEWVLEGT
                     MVNEGPSLAYVTFYVNVTDGRAPLLLRMEYRLEPGERRAVEVGLGAHGEATVNATLGC
                     EEGFDSDIRDNIWGARISDPGLDVPMGYVVVPLIGAIIAAAVVIVRRR"
     gene            complement(3594..3666)
                     /locus_tag="PLJ11_03165"
     tRNA            complement(3594..3666)
                     /locus_tag="PLJ11_03165"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Asp,seq:gtc)
     gene            3853..4653
                     /locus_tag="PLJ11_03170"
     CDS             3853..4653
                     /locus_tag="PLJ11_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO03701.1"
                     /translation="MAERKPRVEDYMIREVVHVPVTYTVKQAVDALIASEFHGMPVTE
                     DGRLVGFITAKELLRHYGTPDRPISEIIRKGTVTVNPKMDLDDVARILFRYGLRNVPV
                     IDENGMLLGIISNLDIVRSHIERATPNKVAMIKNFLEAKHGISIMVRRRIVPVEALRP
                     TQKEVYADELLGRKEEIKRGLVEPIIVIQKNDHYLLVDGHHRALASKQMGVKQFSAFV
                     LEPSRDVELGMERSAEEMGLRTLDDVKIIEGAHHPLVEITTRLLKSEQ"
     gene            complement(4660..6957)
                     /gene="ppsA"
                     /locus_tag="PLJ11_03175"
     CDS             complement(4660..6957)
                     /gene="ppsA"
                     /locus_tag="PLJ11_03175"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOO03702.1"
                     /translation="MNISDLGKNDIDIAGGKGANLGELVAAGFNVPPGFVLTTAAYDY
                     FLENSGIKDTIFELLAKVDASSEASLQDTSQRIRDLFDTVEIPSDLKEEILASYKALF
                     KGKRPGLVAVRSSATAEDLPTASFAGQQDTYLNVESPEDLLDKVRRCWSSLFTPRAIY
                     YRITKGFEHSKVKLAVVVQKMINSEISGIMFTVDPNSEMPHIIIEAGYGLGEALVGGK
                     VTPDTYVVDKFHSKILSKRIAKQTWKLVRGKGGECVRADVPDELSSVQKMTDEQILAL
                     AEIGRNIEAHYNRPMDIEWCIEDRVIYVVQARPVTTLSSPGGKGNGRPTDQGLAKSAK
                     VLVKGLGASPGVAGGPVRTVSEEMNLDIVKKGDVLVTTMTSPDMVPAMTRAAAIVTDE
                     GGMTCHAAIVARELGIPCIVGATHATQVLKDGDMVTVDGSMGVVYEGTAEPKKEEAKL
                     AVPIVAPAQVTGTKVYVNLAIPQKAEEVAKLPVQGVGLMRIEFLFTSYIQEHPCALIE
                     KGESKFLVDKLSEGIAMVAKAFYPRPVILRTSDFKTNEYRDMEGGEKFEPHEENPMIG
                     WRGCSRYVSDSYREAFKLELKAIKKVREEMGLKNLHVMLPFVRTIDEVRRITEMMREV
                     GLERNRDFKLYLMAEIPCNIFMAEEFAEWCDGFSIGSNDLTQLTMGADRDSDILGKMG
                     YFDERNEAIKRAINMLIEAAHRKGCTVGICGQAPSVYPEFTEFLVKAGIDSISLNPDT
                     VISTLGIIASAEQRILLEAARKTLR"
     gene            7431..>7851
                     /locus_tag="PLJ11_03180"
     CDS             7431..>7851
                     /locus_tag="PLJ11_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO03703.1"
                     /translation="MRGDAVSTIEIRWHGRGGQGVVTANEILAGAALREGKFIKAFPE
                     FGPERMGAPIRAFARISDRPITVHSQVYFPDVVLIIDPTLLKAGKVTDGLKEGGGVIA
                     NYPDDSAKLMHAVGRSENVHAVNATKIAVEEIGRPMTN"
BASE COUNT         1439 a         2301 c         2574 g         1537 t
ORIGIN      
        1 cattgcggag gagatggtgc agggcgaggg gggcggtggc cttcatcggc aactcccgct
       61 atgggtggta tgccaggggc agcacgtacg gctcctcgca gcggttcgac aaggccttct
      121 tcagccaggt ctatgacggg gacgctcccg agcttggacg cgccctcgcc tattcgaagg
      181 aatcgctgta cacgtccgcc ctctccgcag gcacgatgcg ctgggtgtat ctggagctga
      241 acctccttgg ggacccggag acaagcgtcc atctgtccga acgggcgacc catgacctgg
      301 aggtgcgggg gatcgaggtg gggcggtgca tagcgggcga gcgatgccag gtcaccgtcg
      361 aggtgcggaa cgtgggccag tccatggacg agggggcgct gcgcctcttg gtcgatgggg
      421 agaccctggg gaacgcgccc gtgtcccttg cgcccggcga gggcatgacg gtgatgatgg
      481 gatggacgcc ctccggcgcc gggcgccacg agctggtggc ggttgtggcg tgcgaggggg
      541 acgagagccc ggagaacgat cgcagcgacc ttgaggtgat ggtggaccgc cgggtcacgt
      601 cggatgagca ctggagcggg gaggtggtcc tctcgagcgg cctattgata gctgagggcg
      661 tgaccgtgac cgcccgggac tgctacattc tgttgcggtc ctccccgatg ttgtacaggc
      721 tgaccgtgga cggggccttc ctcatggaca actgcaccta tggcggatcg ccgtcggtcc
      781 tggattccgc agggggcgag ctggcaatga ccgagtgcct gatggagggg gcatcgctgt
      841 cccttgccca gggagcccgc tttggctcgc tctcccttcg gaacaccagc atggtcggcg
      901 gcagcgggtg gcagattgaa ggtgccaacg tggtgatgac caactcatcg gcagtggccc
      961 aggaagggga atggctcatc tccgattcca cggtggccat cgacctcgtg gatggggctg
     1021 gggggcacgg cctccatctc gtgaacgcga gcgggaccgt ttcacggctc tcgtggaccg
     1081 gggggtcctc cgggctgata gtggaacgct gcagcgggat gaccctccac gatctgagcc
     1141 tcgtcggcaa cgctatggat atggacatag agggggatgt gacggcccat ttcatccatg
     1201 acctgaggaa cgtgacgctg accgcgggcc cgttggccgt cctgcatgga ctggatggaa
     1261 caacgttaac gggagcgttc gcctccctgt acctcgtgga atgccacgcc gtcgtggtga
     1321 gggggcagcg attccaaggg gccgggaccg ggctggcgct gatcgagagc catgggatag
     1381 aggtcatagg gaatgccatt gagaactgca cgacggggat ccttgcgatc gggtcgcagg
     1441 gcaccgtctg ggcgaacgac ctcctcgaca acgacgcgca ggtccatcag gcccggtccg
     1501 acctgacctt cgatcggggg tatcccagag gcggcaacca ttggtcggac atgcgcggtc
     1561 tcgatcaaat gtcgggggac gggcaggatg ccatgggatg cgacggcatc ttcgattcgc
     1621 cttacgaagg gggcgcctat gaccggtatc cgaaggtcgg aaggtgcagc tatctctatg
     1681 acagcccgaa cgcttcattc atcatcaatg ccgtccctgc caacagggtg gaccctgtgg
     1741 tcctgaccca ttgcgggggg agcggcatcg gcatagcgaa ctggacctgg gacctgggcg
     1801 acggaaacat ggcctacggg gaccgagcgg tccataccta cctctccctg ggaacggccc
     1861 ctgttctcct gacggtcacc gaccacaagg gatcgacggc cacggccacg ggggaggtcc
     1921 tggtcgtcaa cctgcttccc gattgcggct tcatcgtctc cccctcccgg ccggcgccag
     1981 gggaagctgt ccgcttcgag gaccgttcct cggaccggga cgggagcata tccgcctggc
     2041 gatgggattt cggggatggg gggacctccc tcgaaccgga gcccgagcat acgtaccagg
     2101 aagaggggga atacctggtg tccttgaccg tgatggatgc tgagggtgga agcgccagca
     2161 ctacccaggc gttggcggtg ggcaacgacc ccccggtggc gaccttcgtc tcggccccca
     2221 gccttatcac cgtcctcacg gacgtccgct tcacctccca gagccatgat ccggacggag
     2281 aggtcgtttc atgggcctgg agcttcgggg acggcgccac cggcaccggc agctcggtcg
     2341 tgcaccgcta ttcctcgctg ggcacgttcc aggtgaccct cgaggtctgg gacgagcacg
     2401 gcgcttcgtc ctccgttact gcctccctca cagtgcggaa ctcaaggccg gtggctgact
     2461 tcgaggcgcc gggctcggtg ctgagcctgg aggaggtaca tttcgtggac ctctcctacg
     2521 accttgatgg ccgggtccta ggctggagct gggatttcgg ggatggtggg acgtccaccc
     2581 tcgcctcgcc tgcacatata tacaagaccc cgggggacta cgcggtcacc ctgacggtca
     2641 tggatgaccg gcgggcatcc tccgtcaaga tggcgccagt gaaggtactc aaccgcctcc
     2701 cgcaggtcgc catcgaaggg ccgtccgggg agcattggtc cctggaggag atggagttcc
     2761 atgcgatggc ctcggacatg gatgggacgg tcgcctcctt ccactgggac atgggggacg
     2821 gcagggaact ggaagggcag acggtgtgcc atgcgtacct atgcccgggc gagtacgtgg
     2881 tgaccctcac ctgtgaggat gacgcagggg gcgtggccaa cgccaccatc gtcgtccagg
     2941 tccagaacct gatccccctg gtgggagcga cgtacgagcg atcgttggac catccattgg
     3001 aactgatgtt ccagagcggc gcggaggatc ttgatggaag catcgcttcc tatgactggc
     3061 gctttgggga cgggggatcc tcggcggagg ctgcccccgc gcatcgctac cgtcgggctg
     3121 gggattacac cgtcgtgctc aacgtgaccg acgaccgtgg ggcctgggcc gtggcattcg
     3181 ccaacgtgac agtggtggaa gggagcctat tcctgacagg aatgacctgc gagaaggccg
     3241 acggggaatg ggtgctcgag ggaacgatgg tgaacgaagg cccgtccctt gcctacgtga
     3301 ctttctacgt caacgtcacc gatggacgcg cccccctcct tttgagaatg gaatatcgat
     3361 tggaacctgg cgaacggagg gcggtcgagg tcgggctggg agcgcatggc gaggccaccg
     3421 tgaacgccac gctaggttgc gaggaagggt tcgattcgga cattcgtgac aacatctggg
     3481 gagcgcggat ctccgaccct gggctggacg tcccgatggg ctatgtcgtc gtgcccctga
     3541 tcggcgcgat catcgccgcg gcggtggtca tcgtcagaag gagatgaggg gagcgccctg
     3601 gtcgggattt gaacccgagt cgaagcctcg acagggcttc atgataggcc gctacactac
     3661 cagggcctca acgacctttc cccctatcga atcataatat ttaaccctta ggagatgggc
     3721 gcgctggccc tggagccctc ggaggacgga ggcgcccccg gcagcccatt gtgcccgggg
     3781 caggcacggt ggtcctcgga tgtctccctc ccaaagtatt aatcggagcc ggtggttaga
     3841 tcgggggatc agatggccga acggaagccg agggtggagg actatatgat tcgcgaggtg
     3901 gtccacgtgc ccgtcaccta caccgtcaag caggctgtgg acgccctcat agccagtgag
     3961 ttccatggga tgcctgtgac cgaggatggg cggctcgtgg ggttcatcac cgccaaggag
     4021 ctgctgcgcc attacggcac ccccgacagg ccgatctccg agatcatccg taagggaacg
     4081 gtgacggtca atcccaagat ggaccttgac gacgtggccc gcatcctctt ccggtacggc
     4141 ctcaggaacg tcccggtaat agacgagaac ggcatgctgc tgggcatcat ttccaatctt
     4201 gacatcgtcc gctcccacat cgagagggcc acgcccaaca aggtggccat gatcaagaac
     4261 ttcctggaag cgaagcacgg catctccatc atggtgcgcc gccgcatcgt gccggtggag
     4321 gccctccggc cgacccagaa ggaggtgtac gcagacgagc tcctggggcg gaaggaggag
     4381 atcaagcgcg gcctggtcga accgatcata gtcatccaga agaacgacca ttacctcctg
     4441 gtcgatgggc accaccgggc gctggcctcc aagcagatgg gggtcaagca gttctcggcc
     4501 ttcgtcctgg agccgtccag ggacgtggag ctgggcatgg agcgcagtgc cgaggagatg
     4561 ggattgcgca ccttggacga cgtgaagatc atcgaggggg cgcaccatcc cctggtggag
     4621 atcaccacca ggctgctgaa gagcgagcag tgaggatggt tacctcaacg tcttgcgcgc
     4681 cgcctcgagc aggatgcgct gctcggcgga ggcgattatg cccagggtgc tgatcacggt
     4741 gtccgggttg aggctgatgg agtctatgcc ggccttcacc aggaattcgg tgaactccgg
     4801 atagaccgat ggggcttgcc cgcatatgcc gacggtacag cccttgcggt gggccgcctc
     4861 gatgagcatg ttgatggccc gcttgatcgc ctcgttcctc tcatcgaagt agcccatctt
     4921 tcccaggata tccgagtcgc ggtccgcgcc catggtcagt tgggtgaggt cgttcgagcc
     4981 gatgctgaac ccgtcgcacc actccgcgaa ctcctcggcc atgaagatgt tgcatgggat
     5041 ctcggccatg aggtacagct tgaagtcacg gttgcgctcc aggcccacct ccctcatcat
     5101 ctccgtgatc ctgcggacct cgtcgatggt gcggacgaag gggagcatca cgtgcaggtt
     5161 cttcagtccc atctcctcgc gcaccttctt aatggccttc agctccagct tgaacgcctc
     5221 ccggtaggaa tcggagacgt agcgcgagca ccccctccac ccgatcatgg ggttctcctc
     5281 gtggggctcg aacttctccc cgccctccat gtcccggtac tcgttggtct tgaagtccga
     5341 ggtgcgcagg atgaccgggc gcggatagaa cgccttggcc accatggcga tgccctcgga
     5401 gagcttgtcc accaggaact tggactcacc cttttcgatg agcgcgcatg gatgctcctg
     5461 gatgtaggag gtgaacagga actcgatgcg catgaggccc actccctgta ctggcagctt
     5521 tgcgacctcc tcggccttct gcgggatcgc aaggttgacg tataccttcg tcccagtgac
     5581 ctgggcgggc gccactatcg gcacggcgag cttcgcctcc tccttcttgg gctcggcggt
     5641 cccctcatag accacgccca tgctgccatc cacggtcacc atgtccccgt ccttcagcac
     5701 ctgggtggca tgcgtggcgc ccacgatgca tgggatcccg agctcgcggg ccacgatggc
     5761 cgcgtggcag gtcatgccgc cctcgtcggt gacgatggct gcggccctgg tcatggcagg
     5821 gaccatgtcc ggggaggtca tcgtggtgac gaggacgtcc cccttcttca caatgtccag
     5881 gttcatctcc tcggagacgg tgcggacagg gccccccgcg acaccggggg aggcccccaa
     5941 ccctttgacg aggaccttgg cgctcttggc aaggccctga tcggtcggcc tcccattgcc
     6001 cttcccaccg ggggaggaca atgtggtcac cggcctggcc tggaccacat agatgacccg
     6061 gtcctcgatg caccactcga tgtccattgg gcggttgtaa tgggcctcga tgttgcgtcc
     6121 gatctccgcc agggccaata tctgctcgtc cgtcatcttc tggacgctac tgagctcgtc
     6181 gggcacgtcg gcccttacgc attcgccgcc ctttcccctg accagcttcc aggtctgctt
     6241 ggctatgcgc ttgctcagga tcttgctgtg gaacttgtcc acgacatacg tgtctggcgt
     6301 gaccttgcct ccgaccaggg cctcgccgag gccgtagccg gcctcgatta tgatgtgggg
     6361 catctccgag ttggggtcta ccgtgaacat gatacccgat atctcggaat tgatcatctt
     6421 ctggacgacg accgccagct ttaccttgga gtgctcgaag cccttggtaa tgcgatagta
     6481 gatggcgcgg ggggtgaaca gcgaggacca gcaccggcgg accttatcca acaggtcctc
     6541 tgggctctcg acgttcagat aggtgtcctg ctgtccggca aagcttgcgg taggcaggtc
     6601 ctccgcggtg gcgctagagc ggacggccac caggccgggt cgcttaccct tgaagagggc
     6661 cttgtaggag gccaggatct cctccttgag atccgagggg atctcaacgg tgtcgaagag
     6721 gtccctgatc ctctgggagg tgtcctgcag ggacgcctcg gagctcgcat ccaccttggc
     6781 cagcagctca aatatggtgt ccttgatccc actgttctcc aaaaagtaat cgtatgccgc
     6841 ggtggtcaga acgaatccgg gagggacatt gaagccggcc gccaccagct ctccgaggtt
     6901 tgctcccttg ccaccggcga tgtcgatgtc gttcttgcct aggtcagata tgttcactat
     6961 ccttttcatg ggccgcttca ccactttatg aacgttcgat gcaggcaagt tcacggggcc
     7021 gatggccctt caatgcccta acccaggtgg aagataggta aggagcgtat taatttttct
     7081 aaaccatgat agggcgtgat cgttcggacg cctatcattt tcgtggatcg ggacggagat
     7141 gggccagcgg tcactgggac cgtgtggatc aagagaaatg gaatgtcgaa cgcgccgagg
     7201 ggacgtcgaa agggtatggt tcctgtgatt cctattggaa tttaattcga agcggaaagg
     7261 ggtagcagga tggttatatt atagcccttg atgagattga atttgtaagt gatgttaccg
     7321 caagtatcat gcgtagctcg tattttcgaa gaatttaccc tttctattca tgggtttcgt
     7381 aaacaatttt ctgaaattat tttatattat gacaatttaa agggtttacc gtgcgaggag
     7441 atgctgtgag caccattgag ataagatggc atggaagagg tggccaaggg gtcgtcacgg
     7501 cgaacgagat cttagctggt gctgccctca gggagggcaa gttcatcaag gccttcccgg
     7561 agttcgggcc agagcgtatg ggcgcgccca tccgcgcttt cgcccggatc tcagacaggc
     7621 ccatcactgt gcacagccag gtgtatttcc ctgacgtcgt gttgatcatc gaccccacgc
     7681 tccttaaggc tggcaaggtg acggatggcc tcaaggaggg agggggcgtc atcgccaact
     7741 atccggacga cagtgctaaa ctgatgcatg ctgtagggcg gagcgagaac gtccatgcgg
     7801 tgaacgcgac aaagatcgcc gtcgaggaga tcggccggcc gatgacgaac a
//
LOCUS       DAOSYN010000046         7730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_8064_length_7730_cov_3.825407, whole genome shotgun sequence.
ACCESSION   DAOSYN010000046 DAOSYN010000000
VERSION     DAOSYN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_7730_cov_3.825407"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLJ11_03185"
     CDS             complement(<1..262)
                     /locus_tag="PLJ11_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03704.1"
                     /translation="MSFNSYFAGGPTPKFSAYHIWKAHQVVAKDGPIGRKALAEALQI
                     GEGSTRTILDKMMREGSVESSRLGITITERGKRKLDTSGMEVR"
     gene            381..2003
                     /gene="atwA"
                     /locus_tag="PLJ11_03190"
     CDS             381..2003
                     /gene="atwA"
                     /locus_tag="PLJ11_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HOO03705.1"
                     /translation="MPDGSEVLVILDKVSKSFNGNQVLKDISTTVRTGEVLGLIGRSG
                     SGKSVLINVMRGSHDYKPDSGRVIYRVNHCPSCGWLDLPSPGAKCTKCGKETKVTEID
                     FWAMDERDPLRLAMRGRIAIMLQRTFALYGDLTVIENVFEVIKGMSDEKKVERAIQLL
                     EMVNMTYRTMHIARDLSGGEKQRTVLARQLARDPLLFLADEPTGTLDPETADIVHKAL
                     IKAVKDTGMSMVITSHWPKAINLLSHRAIWLEDGMIKAEGEPREITKAFDVSFVRDKE
                     MEVQTGEPVIRVKNAKKYFYSIVRGVVKAVDDVSFEVAEKEIFGLIGQSGAGKTTLSR
                     MISGITPATHGEVLVRIGDDWIDMSDLGEEGRGRATQYIGIMHQENALYPFNTVLQNL
                     TICLGMKLPQELAKMKAIQVLRGVGFTAKEVDHILYAYPDSLSVGEKQRIALARTLIM
                     EPRFVVLDEPTGTMDPITRQAVAKSVLHSRKELNQTYLIVSHDMDFVLACCDRVALMK
                     SGKIVAIGPPQEVLRGMTQDELEGEESEVCEV"
     gene            2006..2611
                     /gene="mcrC"
                     /locus_tag="PLJ11_03195"
     CDS             2006..2611
                     /gene="mcrC"
                     /locus_tag="PLJ11_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase I operon protein C"
                     /protein_id="HOO03706.1"
                     /translation="MKDSIGRKTRFVECREARGMGVGGSLAQRATISDSGRDVVAIAM
                     GPGKRHITKPVCEITYGLREEGIDTSVIVITAGSGVPLDAVDVSTSSLFGLEPIEVAR
                     IQQFKVMLIHLGNVRNHIIYKARLILRNVDLPAVICCQAPVDFEDFARIGCKTRLVMP
                     KDEDIKTKGTIMEIVTGVVRGTTISQVKLDEIVAKVKRTLP"
     gene            complement(2608..3171)
                     /locus_tag="PLJ11_03200"
     CDS             complement(2608..3171)
                     /locus_tag="PLJ11_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03707.1"
                     /translation="MDREEVRSAVRARMLAMEGVEALVFLDQGFRQEIEAREREAEGN
                     GAVGGLMPFVNTGVWDVLTRSEVFVIVVQPKVELLRPQEHLVHIVDQKGHIIGEYLPE
                     GRRGDDSIMENAYLISEDFIMYGDVDIIGEPYFLIPPMPFSPLEGMPGIKGLVSASIS
                     TLSDEYIRNRLGYGDTKCWTHMVGYDV"
     gene            3321..4928
                     /locus_tag="PLJ11_03205"
     CDS             3321..4928
                     /locus_tag="PLJ11_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HOO03708.1"
                     /translation="MRAVINGREKELPAGATVAQAIEGEPYLPGSSVALIRPMDAQRK
                     LTNEYMVKTPRGCFIIELNDSRYAEAWKTLYHNVVGRSLRWQTSKLHAFGSFPTQFEK
                     GRGVSHYRRYDCFFALGGYDVSTTYVMIARTDMDEDLGLMDAAFGRVTRGRYVLDLLQ
                     EGDVIEDIEPVVLRLTSKDALTVTDLNAPVEDGMLIETYVGVKLDDRSPLCCEHFLVV
                     TSEGSLSIDEKGFSYTASEANLDTSLGQEYSDVRRPGTVTVRHRGYQNGKIYFYNEVR
                     QLNRSHNIVGTVTNGNDLMRLVPVKGSVLVQPDPIRIMTIGKTQAEAQAFLASLGKEQ
                     RRTGDTSDDAIVVEQEPELTIYAAKEKEIETMGVPKEKVYAVTLDPEGSPWTVRYFRK
                     ITGLDHKPIGTMKVHFTFEGMPMITFEGDSLLAADLYPERQFEQQSIKGDMAITNMSR
                     PQRGLIGIRLEESEEFGPTGEEPHGANMLGRLIGDLDALMDGIQEGDIIYIRPATAEE
                     AVGPRKKMGQKKKASAGKRAVNKSEEG"
     gene            4930..5355
                     /locus_tag="PLJ11_03210"
     CDS             4930..5355
                     /locus_tag="PLJ11_03210"
                     /inference="COORDINATES: protein motif:HMM:NF021401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein 6"
                     /protein_id="HOO03709.1"
                     /translation="MDSEIETRLIFIAPTSDLTPDRIARFIHNLGNEVTVKETCYGVV
                     VEGPKEAVRQTLGEVRKLDPNRIFSKVRAFPVGDVRRCRAHHGSRPGFAQLEKEWKDL
                     CLVEQGLICVEEGERPPTPKKRGRLPIKELRKIVDEVSQ"
     gene            5352..5786
                     /locus_tag="PLJ11_03215"
     CDS             5352..5786
                     /locus_tag="PLJ11_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HOO03710.1"
                     /translation="MRIFIMPPNSLILYDLVERFGHEPLSLMKALRERVTSNEIEAPP
                     LNITPEDVKMGLKYAAIEVPSGIRGRMVVYGPLIDQADAAIFMEDAPYSFGCVGCQRT
                     NELAKLLVRKRKVPILSLDYPSNEEEAKDMVYKVKEFLEGLK"
     gene            5783..7006
                     /locus_tag="PLJ11_03220"
     CDS             5783..7006
                     /locus_tag="PLJ11_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HOO03711.1"
                     /translation="MIKIAQLSCGTEYSSVQPEIERAAETVGAKMVYPDVDMDKIDKA
                     VEEFGFNPKSPQLKLMIARALELANNNYDADAIFIATCFRCAEGALVRTELRRFVQEH
                     VRLPVVTYSFTERTKAAQLLTRMEALVTIVERKELLARERQVGLTAGIDSGSSTTKAV
                     IMRDNEIIGKYWLPTTDVFSSADTALTNALAEAGVRLEQLEGIGTTGYGRYTLGKKFK
                     AKLVQEELTVNSKGAVWLADRQKGEATIIDIGGMDNKAITVRDGIPDNFTMGGICAGA
                     SGRFLEMTAKRLKVEITELGALADQGDHRNVKMNSYCSIFGIQDLVTSLAAGNSLADV
                     ASAACHSVAEQVYEQQLQEIEVRQPIIEVGGTSLVSGLVTAMEHTLGQRPIVPKDPQY
                     IGAVGAALLTSGLLG"
     gene            7011..7583
                     /locus_tag="PLJ11_03225"
     CDS             7011..7583
                     /locus_tag="PLJ11_03225"
                     /inference="COORDINATES: protein motif:HMM:NF021412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HOO03712.1"
                     /translation="MVKFELEGTERYALESYQWLLERILIDMGITTMVERVRLIIRPD
                     QTLFIISMRIKQAGSKRPVSDIADMEAQEGGTFITIKDESYAPRLLASLWRMYGRERV
                     QQLTRLEIFVDGVPVEELSDLKLDPEEEVRSQLLDAMWRLLPEGLKVRNEYYNDNVMT
                     IVATEHGMTVDFLAEAQKVHSSMVQGKGED"
     gene            7598..>7730
                     /locus_tag="PLJ11_03230"
     CDS             7598..>7730
                     /locus_tag="PLJ11_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase family protein"
                     /protein_id="HOO03713.1"
                     /translation="MFDGGVHKLEEMLDLIEDIGGFVLQRIQVQVMVTLTIAIPEEDR
                     "
BASE COUNT         1684 a         2264 c         2456 g         1326 t
ORIGIN      
        1 gccgcacctc catccccgag gtgtcgagct tgcgcttccc cctctcggtg atggtgatcc
       61 ccaagcggga gctttccacc gaaccctcgc gcatcatctt gtccaggatg gtccgggtgc
      121 tcccctcccc tatttggagg gcctcggcca gggccttcct gccgatgggg ccgtccttgg
      181 ccacgacctg atgggccttc cagatgtgat aggcgctaaa cttaggcgtt gggcctcccg
      241 caaagtagga attgaaggac attggcatta taggatggat atataatcca tccatatata
      301 gcttgcacca tccaatcgtt aatgccagtc tttctaaacg gggtaagtat tatatctgca
      361 ttaatcgttc gcaaccaggt atgcctgatg ggtcagaggt cctcgttatc ctggacaagg
      421 tctctaagag cttcaatggg aaccaagtgc tgaaggacat cagtacaacg gtgcgcacag
      481 gggaggtcct cgggctcatc ggcaggtccg ggagcggcaa gtcggtcctc atcaatgtga
      541 tgcgcggctc ccacgattac aagcccgatt cagggcgcgt catctaccgg gtgaaccatt
      601 gcccctcctg cggatggctc gatttgccat cgccgggggc caagtgcacc aagtgcggaa
      661 aggaaacgaa ggtcaccgag atcgacttct gggccatgga cgagcgggac cccctacgcc
      721 tggccatgcg cgggcgcatc gccatcatgc tgcagaggac cttcgccctc tacggggacc
      781 ttaccgtcat agagaacgtc ttcgaggtca tcaagggcat gtcggacgag aagaaggtgg
      841 agcgggccat acagctcctg gagatggtca acatgaccta ccgcacaatg cacatcgccc
      901 gcgacctgtc gggaggggag aagcagagaa cggtcctggc caggcagctg gcgcgcgacc
      961 cgctgctctt cctggcggac gagcccacgg gcaccctgga ccccgagacc gcggacattg
     1021 tgcacaaggc gctcatcaag gccgtcaagg acacgggcat gagcatggtg atcacctccc
     1081 attggcccaa ggccatcaac ctgctctccc atagggcgat ctggctggag gatgggatga
     1141 tcaaggccga gggcgaacct cgggagatca cgaaggcctt cgacgtgagc ttcgttcggg
     1201 acaaggagat ggaggtccag accggggagc ccgtgatccg tgtcaagaac gcgaagaagt
     1261 acttttactc catcgtgcgt ggcgtggtca aggccgtgga cgacgtgtcc ttcgaggtcg
     1321 cggagaagga gatcttcggc ctaatcgggc agagcggggc cggaaagacc accctctccc
     1381 ggatgatctc cggcatcact cccgccaccc atggagaggt gttggtgcgg atcggcgatg
     1441 actggatcga catgtcagac cttggagagg agggaagggg gagggcgacc caatacatcg
     1501 ggattatgca tcaggagaac gccctgtacc ccttcaacac cgtcctgcag aacctcacga
     1561 tctgcctggg catgaagctt ccccaggaac tggccaagat gaaggccatc caggtcctgc
     1621 gaggggtcgg gttcacggcc aaggaggtgg atcacatcct ctacgcctat ccggactcat
     1681 tgagcgtggg ggagaagcag cgcatcgccc tggcaaggac cttgatcatg gagccccgct
     1741 tcgtggtgct cgatgagcca acggggacca tggaccccat cacccgccag gccgtggcga
     1801 agtctgtgct gcactcccgc aaggagctga accagaccta tctcatcgtc agccacgata
     1861 tggacttcgt gctggcctgc tgcgatcgcg tggccttgat gaagagcgga aagatcgttg
     1921 ccatcggacc tcctcaggag gtcctgaggg gcatgaccca ggacgaattg gaaggagagg
     1981 aatccgaggt ctgcgaggtc tgagggtgaa ggactcgatc gggcggaaga cccgctttgt
     2041 ggaatgccgg gaggcccggg ggatgggggt gggcggaagc ctggcccaac gcgcgacgat
     2101 ctccgacagc ggaagggacg tggtggccat cgccatgggc cccggcaagc gccacatcac
     2161 caagccggta tgtgagataa cctacgggct gcgggaagag ggcatcgata cctccgtcat
     2221 agtcatcacc gccggctccg gcgttccctt ggacgcggtc gatgtgtcca caagctccct
     2281 cttcgggctg gagccaattg aggtggcccg catccagcag ttcaaggtga tgcttatcca
     2341 cctcggcaac gtgcgcaatc atatcatcta taaggcgaga ttgatcctga ggaacgtgga
     2401 cctaccggcc gtcatctgct gccaggcgcc agtggacttc gaggacttcg cccgcatagg
     2461 gtgcaagacc cggctggtca tgcccaagga cgaggacatc aagaccaagg ggacgataat
     2521 ggagatagtg accggggtgg tgcgcggcac cacgatcagc caggtcaagc tggacgagat
     2581 cgtggccaag gtcaagcgca ccctgcccta gacgtcgtag ccgaccatgt gcgtccagca
     2641 cttggtgtcc ccgtacccaa gtcgattgcg gatatactca tcagagagag tggagatgct
     2701 ggcgcttacc agccccttga tcccgggcat cccctccaag ggggagaagg gcatgggcgg
     2761 aatcaggaag tagggctctc cgattatgtc cacatccccg tacatgatga agtcctcgct
     2821 gatcaggtag gcgttctcca tgatgctatc atctcccctt cgtccctctg ggaggtattc
     2881 accgatgata tgccccttct gatcaacgat gtgcacgaga tgctcctgcg gacgaagaag
     2941 ctcgaccttt ggctgcacga ctattacgaa gacctcgctc cgggttagca catcccatac
     3001 cccggtgtta acgaaaggca tcagccctcc gaccgcccca ttcccctcgg cctcgcgctc
     3061 ccgcgcctcg atctcctgcc gaaagccttg gtccaggaac accagggcct ccaccccttc
     3121 catcgccaac atcctcgccc gcacggcgga acggacctct tccctgtcca cgagagcatt
     3181 tagcgcatcc catctattca taccttccat gatgcttgag caggcccaag ggatggtgga
     3241 agggccgaat ctcggtggcc agatggaacg agacaagaat aaccttatta ttgccgcact
     3301 gcatgcctac gagggacaag atgagagcgg ttatcaatgg gagggaaaag gagctgcctg
     3361 cgggggcaac ggtcgcccag gccatcgagg gagagccgta cctgcccggg tcctcagtgg
     3421 ccctcattcg ccccatggac gcccagcgca agctcaccaa tgagtacatg gtcaagaccc
     3481 cccggggctg cttcatcatc gagctgaacg atagcaggta cgccgaggcg tggaagaccc
     3541 tctaccacaa tgtggtgggg cgctccctgc ggtggcagac aagcaagctg cacgccttcg
     3601 gctccttccc gacgcagttc gagaagggca ggggcgtcag ccattatagg cgatacgatt
     3661 gcttcttcgc ccttggagga tatgacgtca gtaccaccta tgtcatgatt gccaggacgg
     3721 acatggacga agacctgggc ctgatggacg cagcgttcgg cagggtcact cgcggtcggt
     3781 acgtcctgga cctcctgcag gagggggacg tcatcgagga catcgagccc gtggtgctgc
     3841 gcctgacctc caaggacgcc ctgacggtca cggacctcaa tgcgccggtg gaggatggca
     3901 tgctcatcga gacgtatgtg ggggtgaagc tcgatgacag gtccccgctc tgctgcgagc
     3961 atttcctggt cgtgacaagc gaaggcagcc tttccatcga tgagaagggg ttcagctaca
     4021 ctgcgagcga agcgaacctc gatacctcct taggccagga atatagcgac gtgcgccgcc
     4081 ccggcaccgt cacggtgcgg cacaggggat accagaacgg caagatctat ttctacaacg
     4141 aggtgcggca gctcaaccgc tcgcacaata tcgtggggac ggtgaccaac gggaacgacc
     4201 tgatgcgcct ggtcccggtg aagggcagcg tgctggtcca gccggacccg attcgcataa
     4261 tgaccatcgg caagacccag gccgaggccc aggctttcct ggcctccctg gggaaggagc
     4321 agaggcgcac cggggacaca tccgacgatg ccatcgtcgt cgagcaggag ccggagctga
     4381 ccatctacgc cgccaaggag aaggagatcg agaccatggg cgttcccaag gagaaggtct
     4441 acgcggtcac cctggacccc gaagggtccc cctggaccgt gcgctacttc cggaagatca
     4501 ccgggctgga ccacaagccc atcggtacca tgaaggtcca tttcacattc gaggggatgc
     4561 ccatgatcac cttcgagggc gattccctgt tggccgcaga cctctatccc gagaggcagt
     4621 tcgaacagca gtcgatcaag ggggacatgg ccataaccaa catgtcccgc ccccagcgcg
     4681 ggctgatagg catacgcctc gaggaaagcg aggagtttgg ccccacgggc gaagaaccgc
     4741 atggggccaa catgctcggc cgcctcatcg gcgatctcga cgctcttatg gacggcatcc
     4801 aggaaggaga tatcatctac attcgaccag caacggccga ggaggccgtc ggaccgagga
     4861 agaagatggg gcagaagaaa aaggcctccg ctggaaagag ggccgttaac aaatcagagg
     4921 aggggtagga tggacagtga aatagagacg agacttatat tcatagcccc cacctccgac
     4981 ctcaccccgg accggatcgc ccgcttcatc cataacctgg gcaacgaggt cacggtcaag
     5041 gagacctgct acggcgtggt ggtggagggt cccaaagaag cagtacgcca aaccctgggc
     5101 gaggtccgca agctggaccc caatcgcatc ttctccaagg tgcgagcgtt cccggtgggg
     5161 gacgttcgcc ggtgccgggc ccatcacggc tccaggccag gcttcgccca gctggagaag
     5221 gaatggaagg acctctgcct ggtggagcag ggcctcatat gcgttgaaga gggagagagg
     5281 ccacctaccc ccaagaagcg agggaggctt ccgatcaagg agcttaggaa gatcgtcgac
     5341 gaggtgtcgc aatgaggata ttcataatgc cgccgaacag cctgatattg tacgacctgg
     5401 tggaaaggtt cggccatgag ccgctcagcc tgatgaaggc ccttagggag cgcgtcacgt
     5461 ccaatgagat agaggcgccc ccgctcaaca tcaccccgga ggatgtaaag atggggctca
     5521 aatacgccgc catcgaggtg ccatccggca tccgaggacg gatggtcgtg tacggaccgc
     5581 tcatagacca ggccgacgcg gcgattttca tggaggacgc cccgtacagc ttcggctgcg
     5641 tcggctgtca acggacgaac gagttggcca agctgctggt ccgcaagcgc aaggtgccca
     5701 tcctttccct ggactacccg tccaacgagg aggaggccaa ggacatggtg tacaaggtaa
     5761 aggagttcct ggaggggctg aaatgatcaa gatcgcccag ctttcctgcg gtacggagta
     5821 tagctccgtc cagcccgaga tcgagcgggc cgcggagacc gtgggggcca agatggtcta
     5881 tccggatgtg gacatggaca agatagacaa ggcggtcgag gagttcgggt tcaaccccaa
     5941 aagcccccag ctcaagctca tgatcgcccg ggcgctcgag ctcgcgaaca acaactacga
     6001 tgcggacgcg atcttcatcg ccacctgctt ccgctgcgcg gagggcgccc tggtgcgcac
     6061 cgagctgagg aggttcgtgc aggagcatgt gcggctgccg gtcgtcacct attcgttcac
     6121 agagagaacg aaggccgccc agctcctgac caggatggag gccttggtga cgatagtgga
     6181 gaggaaggaa ctgctggcga gggagcggca ggtcgggctc accgccggaa tagactcggg
     6241 gtcctcgacc accaaggcgg tcatcatgcg ggacaatgag atcatcggaa aatattggct
     6301 gccgaccacc gatgtcttct cctcggcgga cacggccctc accaacgcat tggcagaggc
     6361 aggcgtgcgg ctcgagcaat tggagggaat aggtacaacg ggctacggcc ggtacaccct
     6421 gggcaagaag ttcaaggcca agctggtgca ggaggaactg accgtcaact ccaagggcgc
     6481 cgtctggctc gcggacaggc aaaaggggga ggccaccatc atcgacatcg gggggatgga
     6541 caacaaggcc atcaccgtgc gcgatggcat cccggacaac ttcaccatgg ggggcatatg
     6601 cgcgggcgcc tccggccggt tcctggagat gaccgccaag cgcctgaagg tggagatcac
     6661 cgagctcggg gcgctcgcgg accaaggcga tcacaggaac gtcaagatga actcctactg
     6721 ctccatcttc ggcatccagg acctggtcac atccctggcc gcgggcaact ccctggccga
     6781 tgtggcctcc gcggcgtgtc acagcgtggc ggagcaggta tatgagcagc agctgcagga
     6841 gatagaggtg cgccagccca tcatcgaagt gggcgggacc tccctagtgt cgggattggt
     6901 caccgccatg gagcataccc tggggcagag gccgatcgtg ccgaaggacc cccagtacat
     6961 cggagcggtg ggcgccgcgc tgctgacctc cggcctgctg gggtgagggc atggtgaagt
     7021 tcgagctgga ggggacagag cgctacgccc tcgaatccta ccaatggctg ctggaacgga
     7081 tcctcatcga catggggatc acgaccatgg tggagcgcgt gcgcctgatc atccgccccg
     7141 accagaccct cttcatcatt tccatgagga tcaagcaggc agggagcaag cgcccggtga
     7201 gcgacatagc ggatatggag gcccaggagg gagggacgtt cataaccatc aaggacgaat
     7261 cgtacgcccc ccggctgctt gccagcctgt ggaggatgta cgggagggag cgggtgcagc
     7321 agctcacgcg gctggagatc ttcgtggacg gcgtgccggt ggaggagctt tccgacctca
     7381 agctcgatcc cgaggaggag gtccgctcgc agctcctgga cgccatgtgg aggctcctgc
     7441 cggagggact gaaggtgagg aacgagtact ataacgacaa cgtcatgacc atcgtggcga
     7501 ccgagcacgg catgacagtg gacttcctgg ccgaggccca gaaggtccat tcgagcatgg
     7561 tccagggaaa gggggaggac tgatgtacga ggtcctgatg ttcgatggcg gggtgcacaa
     7621 gctcgaggag atgctcgacc tgatagagga catcggcggg ttcgtgctgc aacgcatcca
     7681 ggtccaagtg atggtcacat tgaccatagc cattccggag gaggaccgcc
//
LOCUS       DAOSYN010000047         7715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_8084_length_7715_cov_3.821671, whole genome shotgun sequence.
ACCESSION   DAOSYN010000047 DAOSYN010000000
VERSION     DAOSYN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8084_length_7715_cov_3.821671"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLJ11_03235"
     CDS             <1..836
                     /locus_tag="PLJ11_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03714.1"
                     /translation="LLGGPEGYSLLRNGVPFIDELELRPVLYHAPEQAFFNLGQECVF
                     DCLFCTSRRLGKDFAKDLDLDRMVAMVLEADRKGELKAVAFTSAVVGSVERTVERMVR
                     AVEMVRRALPRVPIGVEPYVSGFEDIDRLHEAGADELKLNIESYDARIVRAVCPKLEL
                     PRQLELLRYGVQVFGRGKVASNILIGLGESDTNVLEGVAELAGMGVVPSIRALKVNPL
                     NRPALEAALGGLEPVTPERLLRLNAEAKKILMHHGLSTMTFRTMCHECGCCDLVPFKD
                     L"
     gene            925..1617
                     /locus_tag="PLJ11_03240"
     CDS             925..1617
                     /locus_tag="PLJ11_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOO03715.1"
                     /translation="MVSENVEEYLECLWELTQDGTPARTSDIAKRMDVSPASVTEMMQ
                     RLDSQGYISYERYKGATLTEKGLRIGEAIKRKHRLLERFLVDVLGIEKERSHEEACRL
                     EHMLSDESERRISEMMNNPSTCPDGDPIPAMDRLRPSGNEGTVGLSEMREGEEGTISH
                     LVCDDPQKIRRIIAMGFVPERPLIVEDRLPMGGPVLVKVGDSRVALAKEYADLVHVRT
                     RNRARHRHHGDR"
     gene            1617..3524
                     /gene="feoB"
                     /locus_tag="PLJ11_03245"
     CDS             1617..3524
                     /gene="feoB"
                     /locus_tag="PLJ11_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOO03716.1"
                     /translation="MEVLLVGNPNVGKSVIFSRLTGVGAISSNYPGTTVDYLKADMMV
                     NGKKVTFIDLPGLYSLTGGTEDQRVAAQMLRSASPDCVLLVADATRLEPSLVLLFQLI
                     ELGHPVLVAMNMMDSARKRGELDIARLSAILDVPIVPTVAITGEGMGELLRAIEGGQA
                     RRSAFRVRYDPRIEAYLEVLRLGLANFKLEHPVRGVAIKLLEGDEAFAEGVPEYILTQ
                     VDRLREEFRKEHGEDLEVHINRDRYGEAGRILSEVYRPKVQPRTRGQRLSDLTLRPRT
                     GVPILLAVLTLLLAAIIGLGSWLEEALLTAYASVTGTFFADLAEMIGGELGTALASGL
                     DLSLQAILAVVVPYILLFYLLLAVLEDSGYLPRVVVLMDGVMHRIGLHGQAIIPMLVG
                     MGCNVPAILATRTVESRRERLILGTIIVMAVPCSAQMAVIVGTVGSYGGPAYVLAILA
                     ILFGLVLLLGRLLHRALRFEPTGLMIEIPDLAVPRPRNVAVKTWMRIKEFFVLAFPIL
                     LAGSIALELLAAYGVLAKVVEPLAFFTEGFLGLPAITIIALLFGVLRKEMSFQLLVVL
                     FGTADLAAVMTVQQLFVFALVMATFMPCLSTFAVMIREYGAKGTVLVALASLALAFSL
                     GGAANLLLTYL"
     gene            complement(3649..5334)
                     /locus_tag="PLJ11_03250"
     CDS             complement(3649..5334)
                     /locus_tag="PLJ11_03250"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOO03717.1"
                     /translation="MSDNDLEATVRKYALQNAVLYSGKANPKAVQGKVLAEAPELRSR
                     VKEVLPLIASVVEKVNSMPIAEQQRELEAIDASLLRREKGERRTGLPVLPGMERGKVV
                     MRFAPGPSGPLHLGHTRVAILNDEYCRTFDGTFINRIEDTNPERIDPAAYDMIPEDLE
                     WLGVKVHRTVVQSDRFELYYDIARQLIELGRAYVCTCPAEEWRALKEKGVPCPHREED
                     ASTAMEKFERMLAGQYGEEEAIFVVKTDLAHPNPAIRDFVGLRIVTSTPHPLKGSRYC
                     AYPMMNFSVAVDDHLLGLTHVLRGKDHLNNTLRQEYIFNYLGWKRPHYTHYGLVHVPD
                     AILKTSTVGKGIKAGEYAGWDDVRLGTVRALAKRGFRPEAIRRFWVDCGVKETDIEFS
                     WDNLYAYNKDMVDGTANRYFFVWDPVPLEVHGVEELRARAPRHPDHPERGNREFHLTA
                     PITLYVVRKDLEAAMEAGRVRLKDLGNVEVRDGKAFYQGNDLSVLKEGYRIIHWVPAG
                     SVPASVVMPTGESRDGLVENVPKEEEGNVVQFERFGFARVERTAPKLLAYFAH"
     gene            5423..6682
                     /locus_tag="PLJ11_03255"
     CDS             5423..6682
                     /locus_tag="PLJ11_03255"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOO03718.1"
                     /translation="MAVRECLDRMEGVRTVDRKVPWDLEPTRELLKDQTRPVLFTDLN
                     GGMAAGNLWSERGRVESALGLPRGGLTKALLEAMARPRPVEEVEEDAWTARGSFDLGA
                     LPIPKFYPEDGGRYITAGVAIAKLDGKVNVSFHRLMLLGRDRFAIRLVPRHLFTMHRL
                     AQERGEELPIAFAVGLCPSVLLAGAVSTDYEVNELEIASALREIGGLGPLRVRRMGAL
                     QVPVDADYVFEGRLTQETVNEGPFVDISGTYDSVRRQPVFQVDRLYQRRDAILQLVLP
                     GGLEHYNLMGLPREPVILRTVRQVVPRVHAVRLTEGGCCWLHGVISITKNKEGDAMNA
                     AMAAFTGHPSMKQVIVVDEDVDIYDDRQVEWAVATRFQASRGLLVVNNAAGSSLDPSA
                     EGTTSKVAIDATRPLGDAKGFERARLP"
     gene            complement(6683..7471)
                     /locus_tag="PLJ11_03260"
     CDS             complement(6683..7471)
                     /locus_tag="PLJ11_03260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO03719.1"
                     /translation="MSGEDLDNITKELRFHGGKWATAHGSYFSDPAKAWPLVDSALRA
                     ASLARPDVIADIGGGTGFLLGELRARLDGAAPRLVNVDVSPQQLAVCQRRGIDPLLCS
                     LLDLRREAVAAEGQRLMLLSRSVMHYFGREGQDRFLAALRSVLREGELLVQQAACFAT
                     PAEARCMNLLYPMMGVDKFYMTPEELVAAHERNGLEVLSVRRAPPLDLRSSDLAERYA
                     LDEATVERLCQILLDHGMDRSGFAVGDRRFRGPFTYRIVTARAV"
BASE COUNT         1261 a         2539 c         2613 g         1302 t
ORIGIN      
        1 agctgctcgg cggtccggag ggctattccc tgctcaggaa cggtgtaccg ttcatagacg
       61 agctggagct caggccggtg ctctatcacg cgccggagca ggcgttcttc aacctggggc
      121 aggagtgcgt gttcgactgc ctgttctgca cctcccggag gctggggaag gatttcgcga
      181 aggacctgga cctggaccgc atggtggcga tggtcctgga ggccgaccgc aagggcgagc
      241 ttaaggcggt ggcgttcacc tccgcggtcg tcggttccgt ggagcggacg gtggagcgca
      301 tggtgcgcgc cgtcgaaatg gtgcgcaggg cgctcccgcg cgtccccata ggcgtcgagc
      361 cgtatgtgag cgggttcgag gacatcgacc ggctgcacga ggcgggggcg gacgagctca
      421 agctgaacat cgagtcctac gacgcccgga tcgtccgggc ggtgtgcccg aagctcgagc
      481 tgccgaggca gctggagctc ctgaggtacg gggtccaggt gttcggccgc ggaaaggtcg
      541 cctcgaacat cctcatcggg ctcggggaat cggacaccaa cgtcctggag ggcgtggccg
      601 agctcgcggg catgggcgtg gtgccgagca tccgggcgct gaaggtcaat ccgctcaacc
      661 gccccgccct ggaggcggcg ctcggggggc tggagccggt gacgccggag cggctgctgc
      721 gcctgaacgc cgaggcgaag aagatcctca tgcaccacgg cctgagcacg atgaccttcc
      781 gcaccatgtg ccatgagtgc gggtgctgcg acctcgtgcc cttcaaggac ctctgaggct
      841 ttaggctccc cttttcaaag ggcgtatggc caattttagg ccttaagaaa tctataaata
      901 tgaataatta ggtagaccta aatcatggtc agcgagaacg tcgaggagta cctcgagtgc
      961 ctgtgggagc tgacgcagga cgggacgccc gcgcgcacct cggacatcgc aaagaggatg
     1021 gacgtatccc cggcaagcgt gaccgagatg atgcagcgcc tggactcgca ggggtacatc
     1081 tcctatgagc ggtacaaggg ggcgacgctg accgagaagg gcctccggat aggggaggcg
     1141 atcaagcgca agcaccgcct gctcgagcgg ttcctcgtcg acgtcctcgg catcgagaag
     1201 gagcgaagcc acgaggaggc gtgccggctg gagcacatgc tctcggacga gagcgagcgg
     1261 cgcatctcgg agatgatgaa caaccccagc acgtgcccgg acggcgaccc catccccgcg
     1321 atggacaggc tccggccctc ggggaatgag gggacggtcg gcctgagcga gatgagggag
     1381 ggggaggagg ggacgatctc ccatctcgtg tgcgacgacc cgcagaagat ccggaggatc
     1441 atagcgatgg gcttcgttcc cgaacgcccc ctgatcgtgg aggacaggct gccgatgggc
     1501 ggccccgtcc tggtcaaggt gggcgacagc cgggtcgccc tcgcgaagga gtacgccgac
     1561 ctggtgcacg ttcgtaccag gaaccgggcg cgccaccggc accacggtga taggtgatgg
     1621 aggtcctcct cgtcggcaat cccaacgtgg ggaagagcgt gatcttcagc cgcctcacgg
     1681 gggtcggggc catctcctcg aactacccgg gcaccaccgt cgactacctc aaggccgaca
     1741 tgatggtcaa cgggaagaag gtcacgttca tcgacctgcc cgggctctat tccctgacgg
     1801 gcggcacgga ggaccagcgc gtggcggcgc agatgctccg ctccgcctcc ccggactgcg
     1861 tgctgctggt cgcggacgcc accaggctgg agccgtcgct cgtgctcctc ttccagctca
     1921 tcgagctcgg ccatcccgtg ctggtcgcga tgaacatgat ggactccgcc cgcaagcgcg
     1981 gggagctgga catcgcaagg ctctcggcca tcctcgacgt cccgatcgtc cccaccgtgg
     2041 cgatcaccgg cgagggcatg ggggagctgc tgagggccat agagggcggg caggccaggc
     2101 ggtcggcctt cagggtgagg tacgatccgc gcatcgaggc gtacctggag gtgctgcgcc
     2161 tcggcctcgc caatttcaaa ctggaacacc cggtccgagg ggtcgccatc aagctcctgg
     2221 agggggacga ggcgttcgcg gagggcgtgc cggagtacat cctcacgcag gtcgaccggc
     2281 tgagggagga gttccggaag gagcatggcg aggacctcga ggtgcacatc aaccgggacc
     2341 ggtacgggga ggcggggcgc atcctctcgg aggtctaccg ccccaaggtc cagccacgca
     2401 cccgcgggca aaggctctcc gacctcacgc tgaggccgcg caccggggtc ccgatattgc
     2461 tcgctgtgct gacgctcctc ctcgcggcga tcatcggcct cggctcctgg ctggaggagg
     2521 ccctgctgac cgcgtacgcc tcggtgactg gaaccttctt cgccgatctg gccgagatga
     2581 tcggagggga gctggggacc gccctggcgt ccgggctcga cctgagcctg caggcgatcc
     2641 tggcggtggt cgtcccctac atcctcctct tctacctgct cctggcggtg ctcgaggact
     2701 caggctacct cccgcgcgtc gtggtgctga tggacggcgt catgcaccgt atcggcctgc
     2761 acgggcaggc gatcatcccg atgctggtgg ggatggggtg caacgtgccg gccatcctgg
     2821 ccacccgcac agtggagtcc aggcgcgagc ggctcatcct agggacgatc atagtgatgg
     2881 cggtgccgtg ctcggcgcag atggcggtca tcgtgggcac cgtgggcagc tacggcgggc
     2941 cggcgtacgt gcttgccatc ctggccatcc ttttcggcct ggtgctcctc ctcggccgac
     3001 tgctccaccg cgcgctccgg ttcgagccga ccgggctgat gatcgagatc cccgaccttg
     3061 ccgtcccgcg cccccggaac gtggcggtga agacctggat gcgcatcaag gagttcttcg
     3121 tcctcgcctt cccgatcctc ctcgccggca gcatcgccct ggagctgctg gccgcctacg
     3181 gggtcctggc caaggtggtc gagcccctgg ccttcttcac cgagggcttc ctgggcctcc
     3241 cggccataac gatcatcgcc ctcctcttcg gcgtcctgcg gaaggagatg tccttccagc
     3301 tgctcgtcgt cctcttcggc accgcggacc tggcggcggt catgaccgtg cagcagctct
     3361 tcgtgttcgc cctggtgatg gcgaccttca tgccctgcct gtccaccttc gcggtgatga
     3421 tcagggaata cggagcgaag ggcacggtgc tcgtcgccct ggcctcgctc gccctggcgt
     3481 tctccctagg aggggccgcg aaccttctcc tgacatacct ctgatgcgtt catggcaaag
     3541 gcggaccaaa ggcaggggca aggcgccccc ggctccgctt ccacgcctcc agctcgcatg
     3601 cgaatggccc cgggaccttc gaaccaaggg accgcccgcc ccgccagctc aatgagcgaa
     3661 gtacgccagg agcttaggcg ccgtcctctc cacccgcgcg aagccgaagc gctcgaactg
     3721 gacgacgttc ccctcctcct ccttcggcac attctcgacc agcccgtccc tgctctcgcc
     3781 cgtcggcatg acgaccgacg cggggacgga acccgcgggc acccaatgga ttatgcgata
     3841 cccctccttc agcacggaga ggtcgttccc ctggtagaag gccttcccgt cccggacctc
     3901 cacgttcccc aggtccttca gccggaccct cccggcctcc atggccgcct ccaggtcctt
     3961 ccgcaccacg tagagggtga tcggggccgt gaggtggaac tccctgttcc cccgctcggg
     4021 gtggtccggg tgcctcggcg ccctggcgcg gagctcctcc accccgtgca cctcgagcgg
     4081 cacggggtcc cagacgaaga aatagcggtt ggcggtcccg tccaccatgt ccttgttgta
     4141 cgcgtacagg ttgtcccacg agaactcgat gtccgtctcc ttcacgccgc agtccaccca
     4201 gaaccgccgg atcgcctcgg ggcggaaccc gcgcttcgcc agggcgcgga cggtgcctag
     4261 gcgcacgtcg tcccacccgg catactctcc cgcctttatc cccttcccga cggtggaggt
     4321 cttcaggatg gcgtcgggca cgtgcaccag gccgtaatgg gtgtagtgcg gccgcttcca
     4381 gccgaggtag ttgaagatgt actcctggcg caaggtgttg ttcaggtggt ccttccccct
     4441 gaggacgtgg gtgaggccca ggaggtgatc gtccaccgcc acgctgaagt tcatcatcgg
     4501 ataggcgcag tacctgctgc ccttgagcgg atggggggtc gaggtgacga tgcgcagccc
     4561 cacgaagtcc cggatggccg ggttcgggtg cgcgaggtcg gtcttgacca cgaagatcgc
     4621 ctcctcctcc ccgtactggc cggcaagcat ccgctcgaac ttctccatgg cggtcgaggc
     4681 gtcctcctcc ctgtgcgggc atggcactcc cttctccttg agcgccctcc attcctccgc
     4741 ggggcatgtg cacacgtacg ccctcccgag ctcgatgagc tgccgggcga tgtcgtagta
     4801 gagttcgaag cggtcgctct gcacgaccgt cctgtggacc ttgaccccga gccactccag
     4861 gtcctccggg atcatgtcgt aggcggcggg gtcgatcctc tcgggattgg tgtcctcgat
     4921 gcggttgatg aaggtcccgt cgaacgtccg gcaatactcg tcgttgagga tcgccacgcg
     4981 ggtgtgcccg agatggaggg ggccggacgg cccgggcgcg aagcgcatta cgacctttcc
     5041 tctttccatg cccgggagca ccggcagccc ggtcctccgt tcccccttct cccgccgcag
     5101 cagggaggcg tcgatggcct cgagctccct ctgctgctcc gcaattggca tgctgtttac
     5161 tttctccacc accgaggcga tgagaggcag cacctccttc acccgggagc gcagctccgg
     5221 cgcctcggcc aggaccttgc cctgcaccgc cttggggttg gccttgcctg aatagagcac
     5281 ggcgttctgc agggcgtact tccggacggt cgcctccagg tcgttgtcgg acatcacccg
     5341 cggatagcct gcgccggttc tttaaccttc gtagcccacc cctggggcaa atcggtatat
     5401 ccggggaagc cgatccctgc gcatggccgt ccgggagtgc ttggacagga tggaaggggt
     5461 caggaccgtg gaccgcaagg tcccctggga cctggagccg accagggaac tcctcaagga
     5521 ccaaacgagg cccgtgctgt tcaccgacct caacggcggg atggccgccg ggaacctctg
     5581 gagcgagcgg ggaagggtgg aaagcgccct cggcctgccg aggggagggc tgacgaaggc
     5641 cctcctggag gcgatggccc gtccccgccc ggtggaggag gtggaggagg acgcttggac
     5701 cgcccggggg agcttcgacc tcggcgccct gcccattccc aagttctacc cggaggacgg
     5761 cggccgctat attacggccg gggtggccat agccaagctc gacgggaagg tcaacgtctc
     5821 cttccaccgc ctgatgctcc tcggcaggga ccgcttcgcc atccggctgg tgcccaggca
     5881 cctcttcacg atgcacaggc tcgctcagga aaggggcgag gagctgccca tcgcgttcgc
     5941 ggtgggcctc tgcccctccg tcctgctggc gggggcagtg tccacggact acgaggtcaa
     6001 cgagctggag atcgccagcg ccctcaggga gatcgggggc ctgggaccgt tgagggtcag
     6061 gcgcatgggg gccctccagg tcccggtgga cgccgactac gtgttcgagg gccggctcac
     6121 gcaggaaacg gtgaacgagg gccccttcgt ggacatctcg gggacgtacg acagcgtgcg
     6181 caggcagccc gtgttccagg tcgaccgcct gtatcagagg cgggacgcga tcctccagct
     6241 ggtgctcccc gggggattgg agcactacaa tcttatgggg ctgccgcgcg agcccgtcat
     6301 cctcaggacg gtgcggcagg tcgtgccgcg ggtccacgcg gtgaggctca cggagggggg
     6361 ctgctgctgg ctgcacggcg tcatctccat caccaagaac aaggaggggg acgccatgaa
     6421 cgcggcgatg gcggcgttca ccggccaccc gtcgatgaag caggtgatcg tggtggacga
     6481 ggacgtcgac atctacgacg accggcaggt ggaatgggcc gtcgccaccc gcttccaggc
     6541 atcccgcggg cttctggtgg tcaacaacgc cgccggctcc tcgctggacc cgagcgccga
     6601 ggggaccacg tccaaggtgg cgatcgacgc gacgaggccg ttgggggacg ccaagggctt
     6661 cgagcgggcg cggctgccct gatcagaccg ccctggcagt gacgatccgg taggtgaagg
     6721 ggccccggaa ccgacggtcg cccacggcga agccgctcct gtccatgccg tggtcgagga
     6781 ggatctggca cagccgttcg acagtggcct cgtccagagc gtacctctcg gcgaggtcgg
     6841 aggagcggag gtccagggga ggggccctcc gtacggaaag gacctcgagg ccgttccgtt
     6901 cgtgcgccgc caccagctcc tccggggtca tgtagaactt gtccacgccc atcatcgggt
     6961 acaggaggtt catgcaccgg gcctcagcgg gcgtggcgaa gcacgcggcc tgctgcacca
     7021 gcagctcccc ctccctcagg accgaacgca gggcggcgag gaagcggtcc tgcccctccc
     7081 ggccgaagta gtgcatcacc gagcgggaga gcagcatgag ccgctgcccc tccgccgcga
     7141 ccgcctcgcg ccggaggtcg aggagggagc agagcagcgg gtctatcccc ctccgctgac
     7201 atacggccag ctgctgcggg gacacgtcca cgttcaccaa tcggggcgcg gcgccgtcca
     7261 gccgggcccg gagttcgccc agcaggaagc cggtgccgcc gccgatgtcg gcgatgacgt
     7321 ccgggcgggc gagggaggcc gccctcaggg cgctgtccac aagcggccag gccttcgccg
     7381 ggtcggagaa gtaggagccg tgcgccgtcg cccatttccc gccgtggaac cggagctcct
     7441 tcgttatgtt gtccaggtcc tccccgctca tcctaacccg catcccgctt cgattattag
     7501 aggtatgcag ggctaaggtt ccgaacgaca ggaaaggaaa ggttccaacg gggcgtcgaa
     7561 ggagggccgt tcgctccatg tgtttgatgc ccgggtggcg ccttcccctg gggcaatgag
     7621 agaagcgcta ataaaaaggc gatctggatt aatcataccc tggctgaatt ccgtaatgaa
     7681 tgattcagta cgaattatta aaataatata attcg
//
LOCUS       DAOSYN010000048         7539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_8309_length_7539_cov_3.952966, whole genome shotgun sequence.
ACCESSION   DAOSYN010000048 DAOSYN010000000
VERSION     DAOSYN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8309_length_7539_cov_3.952966"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1698)
                     /gene="ilvB"
                     /locus_tag="PLJ11_03265"
     CDS             complement(13..1698)
                     /gene="ilvB"
                     /locus_tag="PLJ11_03265"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOO03720.1"
                     /translation="MKGARAVLELLEGQGVDVMFGYPGGVTIPLYDELLDSSIHHVLV
                     RHEQCAAHMADGYSRTTNRTGVCLATSGPGATNLVTGVATAYADSSPMMVLTGQVATA
                     AIGNNAFQEADIFSLMMPITKHNYRVLKPDDLPEAIKRGMGIANSGRKGPVHIDLPVD
                     VLRGDIDPARLNESFPVPAPFEDLSQVLDAAKLLRDAERPVLLVGGGARWSNASSEVQ
                     RLAEMLPAPVITTIMAKAIIPEDHPLSFGMLGMHGRECSRRALLEADVIFAIGARFSD
                     RTIGYDSDVPKETKVIHLDIDPMEAGKNYRTKVRLVGDAKKGLQMVIKALGRPKGESA
                     WGRRVKELHEGCECNIDIDEEPIKPQKVIWELRKAIAPDAFVVTEVGQNQMWAAHFMR
                     LRHPSQFVTSGGMGTMGFGFPASIGVKYAHRDKQVVDVAGDGSFQMVFQELATAMSED
                     LPVVVCLLNNGWLGMVKQWQKLMWNRRYCGTELRNNPDFVKLAEAFGAGGMTITRPSE
                     LADGFRAAFASDVPFVLDIRTDPEEDMLPMVPGGVSSIDGTIRNRCRWNQDAR"
     gene            2057..3013
                     /locus_tag="PLJ11_03270"
     CDS             2057..3013
                     /locus_tag="PLJ11_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOO03721.1"
                     /translation="MEKRGLYRTLLNMLRISYTLPFVMASVTGAAFALTVCDEWLLAA
                     VIPLDIFFFALFANLSNDYFDHRSGTDATRFDFLTKEREEAIKELYDERVYWEGNIFD
                     RGEVSERTGKLVLTLILALAIITAVPIIRHGGPLVIALGAIGLFLAYFYTAPPLNLGA
                     RGLGELDVGVSFFMMAFFTYYVIWPEWSWQMFLIALLVGTSVMLMRFVDQMSGYEAHL
                     KGGERDWCVRLGLERAVTVVGAILLVIHAMQLALVYFHPVFLVLLLTVPMSRRMMKGL
                     HNKEDELRFIRPAPQIVKMVIVSEVLIVISLIVQSALPWTLW"
     gene            complement(2967..3893)
                     /locus_tag="PLJ11_03275"
     CDS             complement(2967..3893)
                     /locus_tag="PLJ11_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03722.1"
                     /translation="MGVMTAIGQTAYYGTWFLKELLGIKRPLATTMIITYHCNLRCKH
                     CQIADGLPMVPQPHSISYQAAVEEMQASYERGARVLFFEGGEPTSWRDGERTLRDLIL
                     AGREVGFFVTGYTTNGTNVLYPESDVISVSLDGPKEVHDAIRCEGVFDKLMANLQTID
                     HPNVFANMVVMPENQHLVRETAQIVKDNPHMNGLMLNFLTPPPESRVLSMEEKRRVVE
                     EVIALKKEGYPILNSNKALHELLMEDYSERCPFWASEFVLPDRSKCYGCPMRGEACKR
                     CGFNAVREYSLITRGSIATIMSMAGRFGLSTK"
     gene            4035..4283
                     /locus_tag="PLJ11_03280"
     CDS             4035..4283
                     /locus_tag="PLJ11_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HOO03723.1"
                     /translation="MASLKIEIMDKIVALMTAAFGFVAALAWNGAISQLIEDWLGTAE
                     DSTLGLLIYAVIVTIVAVVAIVLISRAYGKMKALEERK"
     gene            4417..5430
                     /locus_tag="PLJ11_03285"
     CDS             4417..5430
                     /locus_tag="PLJ11_03285"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOO03724.1"
                     /translation="MKVKVGVNGYGTIGKRVAWAVKEQDDMEIVGVTKTRPSYEASTA
                     LREGHPLYAARAEDVDGFEGAGIKVAGTLDDLLKKVDVIVDCTPGGTGERNKDLYAKA
                     GVKAIFQGGEAHELAGISFNALANYHEAWGASAVRVVSCNTTGLVRTLFPLQQAFGIE
                     RAFASLVRRGADPADKKGAALNAIEPSLKLPTHHGPDVRTVMHGLDIQTMAVVTPTTL
                     MHLHCISVELKGGPSEKEVLDVWASTPRVRLISGKDGIKNTGQVMELAKDLGRPRGDL
                     MDIAIWKDGIRMVGNTLYYYQAIHQESDVVPENVDCVRAMMKMEKNNLASIERTDRAI
                     GLR"
     gene            5916..7148
                     /gene="pgk"
                     /locus_tag="PLJ11_03290"
     CDS             5916..7148
                     /gene="pgk"
                     /locus_tag="PLJ11_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOO03725.1"
                     /translation="MKDHFTMDDLDVKGKTVIYRVDINCPLNRDTLELANDNRIREIL
                     PTLRELVDRGAKVVILAHQSRPGEWDYIPLERHAKRASELLGTEVTYIDDIYGERAKR
                     AIKSMRPGEVIMLRNARDEKSEMDKVDMEQHRQSPMVRELSALADLYISDAFAAAHRS
                     HCSLVGFQAVLPSAAGRLMEKELEALRRVIDSPSHPCVFVLGGAKFADAVKVVERVLR
                     DGIADKVLLVGLVGNAFLKARGIDLGAKSEQVLMNELTEDNLEAAKALMDRYGERIEI
                     PVDVALDDGGVRKEVPVSALPSELQIMDIGRTTAARFADIIAQACTVFISGPAGVIER
                     EEFSVGTKALMEAAVASGAFSVIGGGHTVSAAQRFNCCDRFSYVSTGGGALENYIMGK
                     PLPVVEGLKAAYRRETGK"
     gene            complement(7150..>7539)
                     /locus_tag="PLJ11_03295"
     CDS             complement(7150..>7539)
                     /locus_tag="PLJ11_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO03726.1"
                     /translation="GLKKVKSQIDSGAPKFLQRAVTMALGAYEGGERPQMVKDNIAVY
                     QRRRNVLVTGLRRLGFKVDPPKGTFYLWLKVDGPSLEFAERLLSAGVVVTPGIGFGRN
                     GEGYVRMATTQPVERIEEALERMAKVL"
BASE COUNT         1366 a         2334 c         2398 g         1441 t
ORIGIN      
        1 caatgttcga acctagcggg catcctggtt ccagcggcag cggttgcgga tcgtcccatc
       61 gatgctgctc accccgccag gcaccatcgg cagcatgtcc tcctccgggt cggtccgaat
      121 gtcgagcacg aacggcacgt cggaggcgaa ggcggccctg aacccatcgg ccagctcact
      181 ggggcgggtg atggtcatgc cccccgctcc gaaggcctcg gccagcttga cgaagtcagg
      241 gttgttgcgc agctccgtcc cgcagtagcg cctgttccac atgagcttct gccactgctt
      301 gaccatccca agccacccgt tgttgagcag gcagaccacc acgggcaggt cctcgctcat
      361 ggccgtggcc agctcctgga ataccatctg gaagctgccg tccccggcga cgtccaccac
      421 ctgcttgtcg cgatgggcgt acttgacgcc gatggaggcc gggaagccga agcccatcgt
      481 gcccatccct ccggaggtca cgaactgact gggatgcctc agccgcatga agtgcgccgc
      541 ccacatctgg ttctgcccca cctcggtgac gacgaaggcg tccggggcga tggccttgcg
      601 cagctcccag atgaccttct gaggctttat gggctcctca tcgatgtcga tattgcactc
      661 gcagccctcg tgcagctcct tcacccggcg gccccaggcg ctctcgccct ttggtcggcc
      721 aagggccttg atgaccatct gcagcccctt cttcgcgtcc cctaccaggc gcaccttggt
      781 gcggtagttc ttgccggcct ccatgggatc gatgtccagg tggatgacct tggtctcctt
      841 gggcacatcg ctgtcatagc cgatggtgcg gtcggagaag cgggccccta tggcgaagat
      901 gacgtccgcc tccagcaggg ccctgcgcga acattcccga ccgtgcatgc ccagcatacc
      961 gaacgaaagg ggatggtcct cagggatgat ggccttggcc attatggtcg tgatgaccgg
     1021 cgccgggagc atctcggcca gcctctgcac ctcgctggat gcgttggacc atctcgcccc
     1081 tccgcctaca aggagtaccg gcctctccgc gtccctgagg agcttggccg cgtcaagcac
     1141 ctgggagagg tcctcgaacg gggcgggcac cgggaagctc tcgttcaatc gggctgggtc
     1201 gatatccccc cttagcacat ccaccgggag atcgatatgc accggcccct tgcgcccact
     1261 gttggcgatg cccatgcccc gcttgatcgc ctccggaagg tcgtcaggct tgagcacccg
     1321 gtagttgtgc ttggtgatcg gcatcatcag gctgaagatg tccgcctcct ggaaggcgtt
     1381 gttgccgatg gcggcggtgg ccacctggcc ggtgaggacc atcatggggg aggagtcggc
     1441 ataggccgtc gcgaccccgg tcacgaggtt ggtggccccg ggcccggagg tggccaggca
     1501 cacccccgtc ctgttcgtgg tcctggagta gccatcggcc atgtgggcgg cgcactgctc
     1561 atggcggacg aggacgtgat ggatgctcga atccagaagt tcatcgtaca gcgggatcgt
     1621 gaccccgcct ggataaccga acatgacgtc cacaccctgc ccttcaagca gttccagaac
     1681 agcccttgcg cctttcatac actgatccct caattcgatg gaggaggatg gcctgaacgg
     1741 cctcctctgt cacaagacga caaccgcagt gagcgatggc aaaagcagtg atgtcctctc
     1801 acccgtgttg gtccttgaca cggattaact tcatgccgac gatgatattt aagaatatct
     1861 gacatcccct ccgcccctca gaaggcgtgc tggggatcga caaggagggt gtagagggcc
     1921 gcgagggacc aacgcccgcg gcaggaggac gttacagatg gagcagggac gtggggccca
     1981 gtccccctag ttcgggcgag gcccggccga ggcaaccgtt atatcccctt tcccatcttt
     2041 ccgccacggt gttccgatgg agaagagagg gctgtaccgc acactgctga acatgctcag
     2101 gataagctat actctcccgt tcgtgatggc ctctgtgacc ggcgcggcgt tcgcgctcac
     2161 ggtctgcgac gaatggctgt tggccgcggt gatccccctg gacatcttct tcttcgccct
     2221 gttcgccaat ctcagcaatg actatttcga tcacaggagc ggcacggacg ccacccggtt
     2281 cgatttcctt acaaaggagc gcgaggaggc catcaaggag ctgtacgacg aaagggtgta
     2341 ctgggagggg aacatctttg ataggggcga agttagcgag cggaccggca agctggtcct
     2401 gaccctgatc ctcgcgttgg ccatcatcac cgccgtgccc atcatccggc acggcgggcc
     2461 gttggtgatc gctctcgggg ccatcggcct cttcctggcc tacttctata ccgccccccc
     2521 gctcaacctg ggggcgcgcg ggttggggga gctggacgtg ggcgtctcgt tcttcatgat
     2581 ggcgttcttc acctattacg tcatctggcc ggaatggtcc tggcagatgt tcctgatagc
     2641 gctgctcgtg ggaacctccg tcatgctcat gcgcttcgtc gaccagatgt cggggtacga
     2701 ggcccacctc aagggcgggg agagggactg gtgcgtacgc ctgggcctgg agagggcggt
     2761 gaccgtggta ggcgcgatcc tactggtcat ccacgccatg cagctggcgt tggtttactt
     2821 ccacccagtg ttcctggtcc tcctgctgac cgttcccatg agccggagga tgatgaaggg
     2881 gcttcataat aaggaggacg agctgcgctt tataaggccg gcgcctcaga tcgtcaagat
     2941 ggtcattgtc agcgaggtcc tgatcgtcat ttcgttgata gtccaaagcg ccctgccatg
     3001 gacattatgg tagcgatgct gccccgggtg atcagggagt actcgcgcac cgcgttgaag
     3061 ccgcatcgct tgcacgcctc cccccgcata gggcagccgt agcacttgct tctgtcgggg
     3121 agcacgaact ccgaggccca gaacgggcat cgttccgagt aatcctccat cagcaattca
     3181 tggagggcct tattggagtt gaggatgggg tagccctcct tcttcagcgc gatgacctcc
     3241 tccaccaccc tgcgcttctc ctccatgctg agcaccctgg actccggagg gggggtcagg
     3301 aagttgagca tcagcccgtt catgtgcggg ttgtccttga cgatctgcgc ggtctcccga
     3361 accagatgct ggttctccgg catgaccacc atgttggcga agacgttggg gtggtcgatc
     3421 gtctgcagat tggccatgag cttgtcgaag acgccctcgc accttatggc gtcgtgcacc
     3481 tccttgggcc cgtcgaggga cacggagatg acgtcgctct cggggtacag gacgttcgtc
     3541 ccgttggtgg tgtagccagt gacgaagaac cctacttccc ggccagccag gatgaggtcc
     3601 cgcagggtgc gctcgccatc ccgccaggag gtcggttcgc cgccctcgaa gaacaggacc
     3661 ctcgccccgc gctcgtacga ggcctgcatc tcctccaccg ccgcctggta cgagatgctg
     3721 tgaggctgcg gcaccatcgg aaggccatca gcgatctggc aatgcttgca ccttagattg
     3781 caatgatagg tgattatcat ggtggtcgcg agcggtcgct tgatgccaag cagctccttc
     3841 aggaaccagg tgccgtagta ggcggtctgc cctatagccg tcattacacc catgcccctt
     3901 catccccgcc ccgctatttc aattgtgacc gccaagggta ccgttgcttc ccttgcccgc
     3961 ccatccctgg actaaacaag cattaaataa tggccaggga gataattggc caaccttttt
     4021 caaggaggga cccaatggca agcttgaaaa ttgagataat ggacaagatc gtggccctga
     4081 tgacggccgc gttcgggttc gtcgccgctc tggcgtggaa cggggctatc tcgcagctga
     4141 tcgaggactg gcttgggaca gcggaggatt ctaccctcgg actgctgatc tacgccgtga
     4201 tcgtcactat cgttgcggtg gtcgcgatcg tcctcatctc cagggcgtac ggcaagatga
     4261 aggccctaga agagcgcaaa taggcccgcc gaaccccttt ggaccttttt ttcctgttac
     4321 tttcaattgg gcgttcattg ttcgtatcgc gtccgtggtc ataccaaaac gatgataacc
     4381 ggccgcggcc atctggatga cgtaggaagg ggtcatatga aggtcaaggt cggcgtcaac
     4441 ggctatggca cgatagggaa aagggtggct tgggctgtga aggaacagga cgacatggag
     4501 atcgtgggcg tgaccaagac ccgcccttcg tatgaggcct ccaccgccct gcgggagggc
     4561 catccgctct acgctgccag ggctgaggac gtggacgggt tcgagggggc ggggatcaag
     4621 gtcgctggca cgctggacga cctgctcaag aaggtggacg tgatcgtgga ctgcaccccc
     4681 ggcgggaccg gggagaggaa caaggacctc tatgccaagg cgggggtaaa ggccatcttc
     4741 cagggagggg aggcccacga actggcgggc atttccttca acgcccttgc caactatcat
     4801 gaggcctggg gggcctcggc ggtgcgggtc gtgtcctgca acaccactgg ccttgtccgc
     4861 acccttttcc cgttgcagca ggccttcggg atcgagcggg ccttcgcatc gcttgtgcgc
     4921 cggggggcgg accctgcgga caagaagggg gccgccttga acgccatcga gccttcgctg
     4981 aagctgccga cccatcatgg gccggacgta aggacggtga tgcacgggtt ggacatccag
     5041 acgatggccg tggtcacccc caccaccctc atgcacctgc actgcatctc cgtcgaattg
     5101 aagggcgggc ccagcgagaa ggaggtcttg gacgtgtggg cctccacccc cagggtgagg
     5161 ctcatctccg ggaaggacgg catcaagaac accggccagg tcatggagct ggccaaggac
     5221 ctgggccggc cgcgcgggga cctcatggac attgccatct ggaaggacgg gatcaggatg
     5281 gtggggaaca ccctctacta ttaccaggcc atccatcagg agagcgacgt ggtcccggag
     5341 aacgtggact gcgtccgggc catgatgaag atggaaaaga ataacctggc ctccatcgag
     5401 aggaccgaca gggcgatagg gcttcgctag gccatgggag gggcgtccgg tcgtctccct
     5461 tcaggcgatc gggcgcacca ccctcttcat ggcaaacccg gtcggggctg cgaggcattt
     5521 ggcgatcggg agcccctgcc ggcgcggcgg tcgaggtcca tcgggaccga gaaaggggca
     5581 agcctgccta tgggatgggc gcagggtccc tcaaaagcga cgctaatgcc ttggggacta
     5641 ccgggcattg atcctggaat tccatcgtgg agaccgtggg gaggcacggt cccgaacctt
     5701 ccatcacgta gcctggcagg ttttcccctg cacatttttc tcccttgcgc tccgaacggt
     5761 tcgccgggac ctctcccttt ccgctcggag gggaggtgcc tggacaaggg gggcgatggc
     5821 cccagggcgc ccaggcaaat cattttttat tcaatcgcca tccccgtgct gaggagctgc
     5881 cgctgacagg gtctgaggcc gccggggaat ttgccatgaa ggaccacttc accatggacg
     5941 atctggacgt aaaggggaag accgtcatct atcgcgtgga catcaactgc ccgcttaaca
     6001 gggacacact agagctggcc aacgacaatc gcatccggga gatccttccc accctgcggg
     6061 agctggtgga ccgcggggcg aaggtggtca tcctggccca tcagagccgg ccgggggagt
     6121 gggactacat cccccttgaa cgccatgcca agcgcgcctc ggaactcctt gggaccgagg
     6181 tcacctatat cgatgacatt tacggggaga gggcgaagag ggcgatcaag agcatgaggc
     6241 cgggggaggt catcatgctc cggaacgcga gggacgagaa gagcgagatg gacaaggtgg
     6301 acatggagca gcaccgccag tcccccatgg tccgggagct ttccgccctg gccgacctct
     6361 atatctcgga cgcgttcgcc gccgcccacc gctcccattg ctcccttgtc ggcttccagg
     6421 ctgtcctgcc ctcggccgcc ggcagactga tggagaagga attggaggcc ttgcgccggg
     6481 tcatcgattc cccttcccat ccctgcgtct tcgtgctcgg cggcgccaag ttcgcggacg
     6541 cggtcaaggt cgtcgagcgc gtgctccggg acggcatcgc cgacaaggtg ctgctcgtcg
     6601 ggctggtcgg aaacgccttc ctgaaggcga ggggcataga cctgggagcg aagagcgagc
     6661 aggtcctgat gaacgagctc acggaggaca acctggaggc ggccaaggcg ctcatggaca
     6721 ggtacgggga gcgcatcgag atccctgtgg acgtcgccct ggacgatggg ggcgtccgga
     6781 aggaggtgcc tgtgagcgcc cttccttccg agctgcagat catggacata ggccgcacga
     6841 cagccgccag gttcgcggac ataattgccc aggcctgcac ggtcttcatc tccggcccgg
     6901 caggggtgat cgagcgggag gagttctccg tggggaccaa ggctctgatg gaggcggcgg
     6961 tcgccagcgg cgccttctcc gtcatcgggg gcgggcatac ggtcagcgcc gcgcagcgct
     7021 tcaactgctg cgaccgcttc tcctatgtga gcacgggcgg gggggcgctg gagaactaca
     7081 tcatgggcaa gcccctgccc gtggtcgagg ggctcaaggc cgcgtaccga agggagacgg
     7141 ggaagtagcc tagaggacct tggccatcct ttccagggcc tcctcgatcc gttccactgg
     7201 ctgggtggtg gccatgcgca catagccctc accgttcctc ccgaagccga tcccgggggt
     7261 gacgaccacg ccggcgctca gcagcctctc cgcgaactcc agggagggac cgtccacctt
     7321 cagccagaga tagaaggtcc ccttgggtgg gtccaccttg aagcccagcc tgcgtagccc
     7381 cgtgaccagc acgttcctgc gcctttggta gacggctatg ttgtccttga ccatctgcgg
     7441 cctctctcct ccctcatacg cccccagggc catggtgacc gccctctgca ggaacttggg
     7501 cgcgccagaa tcgatctggg acttgacctt cttcaggcc
//
LOCUS       DAOSYN010000049         7522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_8340_length_7522_cov_3.869693, whole genome shotgun sequence.
ACCESSION   DAOSYN010000049 DAOSYN010000000
VERSION     DAOSYN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8340_length_7522_cov_3.869693"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLJ11_03300"
     CDS             <1..868
                     /locus_tag="PLJ11_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO03727.1"
                     /translation="PILPLHLFKESITTLGSAGLFIMSFGLFGVISFLPLFLQAVVGM
                     SATNSGELLIPMMLGVMATSLASGFLLKRTGYKPWLLAGPPVAALGLYLLSTLHSGSG
                     ETETVLYLIVTGLGMGAVMSNFIVAAQNVTSKKEMGVVSSTMSLFRSIGGTVGVTALG
                     AIVNSRMTTELKANLPEGAMALLPTTDINSIGGILMSPAAANIPTEVLEAIRLSLSNS
                     ITYMFLIGAGIVLSTVVISALVRNVPLKSADQYHEVDVSELGREGPTGPETARKDEAG
                     ETEINTERSASK"
     gene            884..1324
                     /locus_tag="PLJ11_03305"
     CDS             884..1324
                     /locus_tag="PLJ11_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO03728.1"
                     /translation="MALYKNILMPTDGSVHSKAAIAHGLELAKVHDAEVTVLSVIDVS
                     DVTSTLQGVSPGPYNASIQGDVAEAAVKEAADMAIKAGVKVKALVKIGRPAADIIEMS
                     GNYDLIVMGTQGRTGLPHLLIGSVAERVVREARCPVLVTKCKRA"
     gene            1773..1976
                     /locus_tag="PLJ11_03310"
     CDS             1773..1976
                     /locus_tag="PLJ11_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03729.1"
                     /translation="MFKYEYVINWAGQTFKDVIECDGNEDSKREVMRRLKTLGIPSGK
                     YVFVDIVRLDDAKPIIEEELWRA"
     gene            complement(2273..2348)
                     /locus_tag="PLJ11_03315"
     tRNA            complement(2273..2348)
                     /locus_tag="PLJ11_03315"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2312..2314),aa:Gly,seq:gcc)
     gene            complement(2541..5159)
                     /locus_tag="PLJ11_03320"
     CDS             complement(2541..5159)
                     /locus_tag="PLJ11_03320"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOO03730.1"
                     /translation="MAQYEAIPTERKWQRQWKDWELYRFDPESPAPVYSIDNPPRYAS
                     GALHLGHATGYSLIDFAARYHRQRGYNVMFPLCFDVNGTPVEVRVERKYGINKYSVPR
                     QRYIELCEEYANSFIESMTHQFEILGESMDPSLYYQTDAPYYRRITQISFLRMLEKGL
                     AYKGEYPVNWCPRCITALADAEVEYDQNMTKLNYVKFFLADGTGHIIVATTRPELICT
                     CQTIAVHPNDERYRDLVGKDIITPIFGKKVRVIADEKVDPAFGSGIVMICTIGDKTDL
                     EWVMKYKLPLEKGLDEEGRMTALAGPYEGMPVKEARAKVIEDLLASGMITKQEDQLQN
                     RSICWRCHTPIEFLQVKQWFIKIMDFKKEVLAKADEVQWFPEFMKVRLKDWVDSLQWD
                     WVVSRQRYFATPIPVWECQNCDFVLPVREEDCYIDPTIAKPYLDRCPRCGGELKGCPD
                     VFDTWMDSSVSPLYNTFWQRDEERFRRLYPMSLRPQSHDIIRTWAFYTLLRGYHLTGE
                     RPWDHIMIHGFIMAPDGTPMHTSLGNVIDPMPLLETYGADALRYYACTCSLGEDHAFR
                     ERDVVHGRKLCTKVWNLGKLIEMTVRERPERGELRPIDRWILSRFTRTLRAVTDHLDH
                     YDFDKAMREVEGFAWKEFADHYVEMTKHRTRGGDASVRYTLYTVYLGILKMMAPMLPH
                     VTEDAYQPFARMDGARSVHVSKWPEPVLIDEEAERRGDLTKEVIASIRTWKAEKKYPL
                     NKELARVEVVGERAIDLEGYEDDIAQTVRAGAVELLHVADLEKTAVAVRPVHARLGPM
                     FKDRAKEIVEGLMAMDPEEALHQLSADGALELALKDGERVRVTSDLVDVVYELRYQGK
                     AVDTIQVGDLLVVVQV"
     gene            complement(5213..6406)
                     /locus_tag="PLJ11_03325"
     CDS             complement(5213..6406)
                     /locus_tag="PLJ11_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO03731.1"
                     /translation="MVGESIEGLKRKELLRSQIDANKVGDLMRTKFETVGPETPLSDV
                     VAKMNSSDLHEIPVVDGKKLLGVISYQTIIRRKNLVLGTKAKTMVEPTPEIMLDTPLT
                     EVAEHFLSSGFRNLPVTKGRVVQGIITRTAVISVLPPLKEIKAMKVGDVMTTEPHAVH
                     EKDKIKDAMGLMRKLDVRTVPVVDANGKLVGIIGIRDLVQYSWSGPGIKTQGPGDMAG
                     NKDPIELEVRSLMHESPITLTAEDPLPKAIQTMVDRNISTIPVVDEGRMVGIITAYDV
                     LELVASVRQRDMVYTQISGLEEEDRFSLDSMEKEVQAGLAKIAKITRSLLFTIHVSKY
                     NNTGNSAKYSLTGRLNTENSHYMAKAVDWSLPRATEELMDTLERLVKERKEHALDVRM
                     KRGRR"
     gene            complement(6439..>7522)
                     /gene="glyS"
                     /locus_tag="PLJ11_03330"
     CDS             complement(6439..>7522)
                     /gene="glyS"
                     /locus_tag="PLJ11_03330"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOO03732.1"
                     /translation="GVIRLREFNMMEAELFVDPEDKSWPRYGGIKDRKVRLVPNTDGV
                     EVEMGIGEAVDRGIIGNETLAYFIWVTYDFLTAVGIDPARLRFRQHLKAEMAHYASDC
                     WDAEALISFGWTEIVGIADRGCWDLSQHMRFSKADLTAFKRFDEPKEVERDAIRPKFG
                     AIGPRFKGAAGDIKRQMEAMDASAIHDGLVTVIVDGTPVDLDADHYDVVRVREKVNGV
                     RVVPHVIEPSHGLDRILYSCLEHAYAERDGYVTLRLRPAVAPIKVGVFPLMAKDGMEE
                     VAKALDLALREEGITTYYDDSGSIGRRYARMDEVGTPWCVTVDYDTLEKGDVTIRDRD
                     TAEQARVPADDVVAWVKDRLKHGLVC"
BASE COUNT         1321 a         2439 c         2255 g         1507 t
ORIGIN      
        1 tcccatcctc cccctgcacc tgttcaagga gtcgataaca acccttggga gcgccgggct
       61 gttcatcatg tccttcggcc tcttcggagt gatctccttc ctcccgctct tcctccaggc
      121 ggtcgtgggg atgagcgcca ccaacagcgg cgagctcctc atccccatga tgttgggggt
      181 catggccacc agcctggcga gcggattcct gctcaagcgc actggctata agccctggct
      241 attggccggg cctccagtgg ccgctctcgg gctgtacctg ctctccaccc tgcacagcgg
      301 aagcggcgag acggagacgg tcctctacct aatcgtgacc gggttgggca tgggagcggt
      361 catgtccaat ttcatagtgg ctgcccagaa cgtgacctcc aagaaggaga tgggggtggt
      421 gtcatccacc atgagcctgt tccgcagcat cggcggcacg gtaggcgtga cggccttggg
      481 ggccatcgtc aactcccgta tgacgaccga gctgaaggcc aaccttcctg agggcgcgat
      541 ggcgctgctg cccaccaccg acatcaacag cataggcggg atcctgatgt ccccggccgc
      601 ggccaacatc cccacggagg tcctagaggc catacggtta tccctcagca acagcatcac
      661 ctacatgttc ctcatcgggg ccgggatagt gctgtcgacc gtggtcatca gcgccctggt
      721 gcggaacgtc ccgttgaaga gcgccgatca gtaccacgag gtggacgtct cggagctcgg
      781 gagggagggg cccaccggtc ctgagaccgc tcgcaaggac gaggcggggg aaacggagat
      841 caatactgaa aggagcgcaa gcaagtaagg aaaggcgata caaatggcac tctacaagaa
      901 catcctgatg cccaccgacg gcagcgtgca cagcaaggcg gccatagccc acggcctgga
      961 actggccaag gtccacgacg cggaggtcac cgtgctgagc gtgatcgatg tgagcgatgt
     1021 cacctccacc cttcaggggg tgtcgcccgg gccctacaac gcctccatcc agggcgatgt
     1081 ggccgaagcg gccgtcaagg aggccgcgga catggccatc aaggccgggg tgaaggtcaa
     1141 ggccctcgtc aagataggac ggccggccgc ggacataatc gagatgtcgg gcaattacga
     1201 cctcatcgtg atgggcaccc aggggcgcac cgggctcccg catctcctga tcggcagcgt
     1261 cgccgagagg gtggtccgcg aggcgaggtg cccggtgctg gtcaccaagt gcaagcgggc
     1321 gtaggccgct tggtccgcag gacgccattc cgaaggggag gtgctcctcg acggcctcaa
     1381 gggctatcct gaatgcccca ttccccgcct tcagggcgat ggcgggccgt ctcctgagcc
     1441 ataatctctt cgagacaggc cctttcgccc cgaccaggtc ccagatgcat cgtgaaatca
     1501 cggcccagcc ggggcctagg cggtgcctac ggtcctcttg ccacgcaagg cgctgctgga
     1561 gcaaacggct tgagcggtcg acatccccct cgagaatgag gttgacaagg cgaaggaggc
     1621 tgcaatagcc tttttatttt ttatatattt taatattata ttactaccag ccccgcaatt
     1681 cggaacgatg aggtgcaaag ggatggatat agctatatcc cagggaagcg aataccctcg
     1741 gccaaaagca atgagcgatt gagttgggtt ctatgttcaa gtacgaatac gtcatcaact
     1801 gggccgggca gaccttcaag gacgtcatcg agtgcgacgg gaacgaggac tccaagcggg
     1861 aggtcatgcg aaggctgaag acgctcggga tcccgtcggg caagtacgtc ttcgtcgaca
     1921 tcgtccgttt ggatgacgcc aaacccatca tcgaagagga gctctggcgg gcctgaaggg
     1981 gcccttcagg ccagagattc gcccgatcag gcctggccgg tcctcttccc tttctgatcg
     2041 gcaatgccgt tctgggacat cgtcccggca tcgcccatcg atcttccttg atacgcgggc
     2101 gctttcaata gggacctgag ggcttaatgg aagaggggac gggcatggtg cctgaaccat
     2161 ccgtaccgtt caaagcctca tagcgactgt cggatcggca tgggacggtg tctccggcaa
     2221 ggccttcggg aacgcattcc taggacggaa gatgcggagc gaaaaggaag catggtgcgg
     2281 gcgtcgggat ttgaacccga gttatgagct tggcaagctc aagtgataac cagctacact
     2341 acgcccgcgt gcttttgtcg gtattgtcta atcggattta aaggttgccc acacggccat
     2401 atggcccatt caggcatgcg ctaggaacgc ccgcgctcta gcgaaaggaa ggcgcgtcca
     2461 aaagcgccat cgtcccatcg agcatgtcgt cccggatcgg gcaatcctcc gttcccaagt
     2521 tcggtccatc ctcccccgcc tcagacctgg accacgacga gaaggtcccc gacctggatg
     2581 gtgtccacgg ccttgccctg gtagcgcagc tcgtagacga catcgaccag gtcgctggtg
     2641 accctcaccc tttccccgtc cttgagcgca agctcgaggg cgccatctgc gctcagctgg
     2701 tgcagtgcct cctcggggtc cattgccatg agcccctcca cgatctcctt ggcccggtcc
     2761 ttgaacattg ggcccaggcg ggcgtgcacc ggcctgaccg cgacagcggt cttctccagg
     2821 tccgcgacgt gcagcagctc caccgcgccc gcccggacgg tctgggcgat gtcgtcctcg
     2881 tagccctcta ggtcgatcgc gcgttcgccc acgacctcca ccctggcaag ctccttgttc
     2941 aggggatact tcttctccgc cttccaggtg cggatggacg cgatgacctc cttggtgagg
     3001 tcccctctcc gctccgcctc ctcgtcgatg agcaccggct ccggccactt gctcacatgc
     3061 accgatctgg ccccgtccat ccgggcgaag ggctggtacg cgtcctcggt gacgtgcggc
     3121 agcatggggg ccatcatctt cagtatgccc aggtagacgg tgtacagcgt atagcgcacg
     3181 gacgcgtccc ctcccctcgt ccggtgcttg gtcatctcca cgtaatgatc ggcgaactcc
     3241 ttccaggcga agccctcgac ctcccgcatc gccttgtcga agtcataatg gtccaggtga
     3301 tcggtgaccg ccctcagcgt cctggtgaag cggctcagga tccaccggtc gatgggcctg
     3361 agctctcccc gctcgggcct ctccctcacg gtcatctcga tcagcttgcc gaggttccag
     3421 accttggtgc aaagcttcct gccgtgcacc acgtcccgct cccggaacgc atggtcctcg
     3481 ccgagggagc acgtgcaggc gtaatagcgc agcgcgtccg ccccgtaggt ctccagcaac
     3541 ggcatcgggt ctatgacgtt gcccagggag gtgtgcatcg gggtgccgtc gggcgccatg
     3601 atgaacccgt ggatcatgat gtggtcccac ggccgctccc cggtcagatg gtaccccctc
     3661 aggagcgtgt agaacgccca cgtccggatg atgtcatggg actggggccg gagggacatt
     3721 ggatacaacc gcctgaaccg ctcctcgtcc ctctgccaga aggtgttgta taacggggag
     3781 accgaggagt ccatccaggt atcgaacacg tccgggcatc ccttcagctc gcccccgcac
     3841 ctcgggcatc tatccaggta cggcttggcg atggtcgggt cgatgtagca gtcctcctcc
     3901 ctcacaggca gcacgaaatc gcagttctgg cactcccata ccgggatggg ggtggcgaag
     3961 tagcgctggc gggagacgac ccagtcccat tgcagggagt cgacccagtc cttcaaccgc
     4021 accttcatga actccgggaa ccactggacc tcgtcggcct tcgccagcac ctccttcttg
     4081 aagtccatta tctttatgaa ccactgcttg acctggagga actcgatggg cgtgtggcat
     4141 cgccagcata tggagcggtt ctgcagctgg tcctcctgct tcgttatcat ccccgaggcg
     4201 agcaggtcct cgatgacctt ggcccgggcc tccttcaccg gcatcccctc gtacgggccg
     4261 gcgagggcgg tcatgcgccc ctcctcgtcc agccccttct ccaacggcag cttgtacttc
     4321 atcacccact cgaggtcggt cttatccccg atggtgcata tcatgacgat cccggagccg
     4381 aaggcggggt cgaccttctc gtcggcgatg acccttacct tcttcccgaa gatcggggtg
     4441 atgatgtcct taccgaccag gtccctgtac cgctcgtcgt tcgggtggac cgcgatggtc
     4501 tggcaggtgc agatgagctc cggacgggtg gtggcgacga tgatgtgccc cgttccatcg
     4561 gcgaggaaga acttaacgta attcagcttg gtcatgttct ggtcgtactc tacctctgcg
     4621 tccgcgagcg ccgtgatgca gcgggggcac cagttgacag ggtattcgcc cttgtaggcc
     4681 agtcccttct ccagcatcct gaggaaggat atctgggtga tgcggcggta atagggcgcg
     4741 tccgtctgat agtagaggga ggggtccatg ctctccccca ggatctcgaa ctggtgggtc
     4801 atggactcga tgaaggagtt ggcatactcc tcgcacagct cgatgtacct ctggcgcggg
     4861 acggagtact tgttgatccc gtacttcctc tccacgcgga cctccaccgg cgtcccgttc
     4921 acatcgaagc acagcgggaa catcacgttg tagccacgct gcctgtgata ccttgcagcg
     4981 aaatcgatca gggaatagcc ggtggcgtgc cccagatgca gggcgccgga ggcgtaccgg
     5041 ggcgggttgt cgatggaata gacgggggcg ggggactccg ggtcgaacct gtataattcc
     5101 cagtccttcc actgcctctg ccacttcctc tcggtaggga tcgcttcata ttgtgccatg
     5161 gagtggaggg ggaatggggg gtggttctaa aaaaggttgc tgaggggctc cctcaccgcc
     5221 tccccctctt catcctcacg tccagggcat gctccttcct ctccttgacg agccgttcca
     5281 gggtgtccat cagctcctcc gtcgcccgag gcagcgacca atcgaccgcc ttggccatgt
     5341 agtgtgagtt ctcggtgttc aggcggccgg tcagggagta cttggcggag ttgcccgtgt
     5401 tgttgtactt gctcacgtgg atggtgaaga gcagcgatcg ggtgatcttc gcaatcttgg
     5461 ccagcccggc ctgcacctcc ttctccatgg agtccaggga gaagcggtcc tcctcctcca
     5521 gcccggatat ctgggtatat accatatccc gctggcggac ggacgccacc agctccagca
     5581 cgtcgtatgc ggtgatgatc ccgaccatgc gcccctcgtc cacgacaggg atggtggaga
     5641 tgttccggtc caccatggtc tggatggcct tgggcagggg gtcctccgcc gtcagggtga
     5701 tcgggctttc atgcatcagc gaacggacct cgagctcgat cgggtccttg ttcccggcca
     5761 tgtcccccgg cccctgggtc ttgatccccg ggccagacca ggagtattgc accaagtctc
     5821 taatcccaat gatgcccacc agcttcccgt tggcgtcgac caccgggacc gtgcgcacgt
     5881 ccagcttccg catcaggccc atggcgtcct tgatcttgtc cttctcgtgc accgcgtggg
     5941 gctccgtggt catgacgtcg cccaccttca tggccttgat ctccttcagc ggagggagga
     6001 cggagatgac cgccgtccgg gtgataatgc cttgaacgac cctccccttc gtcaccggca
     6061 ggttcctgaa gcccgaggag aggaagtgct cagccacctc ggtcaacggc gtgtccagca
     6121 tgatctcggg ggtgggctcg accatggtct tggccttggt gcccaggacc aggttcttcc
     6181 tccggatgat cgtctggtag ctgatgaccc ccagcagctt cttgccatcc acgaccggga
     6241 tctcgtgcag gtcggacgag ttcatcttgg ccaccacgtc ggagaggggc gtctccgggc
     6301 ctaccgtctc gaacttcgtc ctcatcaggt cgccgacctt gttggcgtcg atctgggacc
     6361 tcagcagctc cttccttttc aatccctcga tgctttctcc taccattctg ctcaccttcc
     6421 tcgtctaaag gatttgggtc agcacaccag tccgtgcttg agcctgtcct tgacccaggc
     6481 gacgacatcg tcggccggca cccgggcctg ctccgcggtg tccctgtccc ttatcgtcac
     6541 gtcccccttc tcgagggtgt catagtcaac ggtgacgcac cagggagtgc ccacctcgtc
     6601 catgcgggcg tagcggcggc cgatggagcc ggaatcgtcg taataggtcg ttatcccctc
     6661 ctcccgcagc gccaggtcca gggccttggc cacctcctcc atgccgtcct tggccatcaa
     6721 cgggaagacc ccgaccttga tgggggcgac cgccggcctc agccgcaggg tgacgtaccc
     6781 gtccctctcc gcataggcgt gctccaggca gctgtacagg atgcggtcca ggccgtggga
     6841 cggttcgatc acgtgcggca cgacccggac gccgttcacc ttctccctca ccctgaccac
     6901 gtcgtagtga tcagcatcca ggtccacagg cgtcccgtcg acgatgacgg tcaccaggcc
     6961 gtcgtggatg gcggaggcgt ccatggcctc catctgccgc ttgatatctc cagccgctcc
     7021 cttgaagcgg gggccgatcg ccccgaactt cggcctgatg gcgtccctct cgacctcctt
     7081 gggctcgtcg aagcgcttga aggcggtcag gtcggccttg gagaaccgca tgtgctggga
     7141 gaggtcccag cagccccggt cggcgatgcc aacgatctcc gtccacccga agctgatcag
     7201 cgcctccgca tcccagcaat cggacgcata gtgggccatc tcggccttga ggtgctgccg
     7261 gaagcgcagc ctcgccgggt cgatgccgac ggctgtcagg aagtcatagg tgacccagat
     7321 gaagtaggcg agggtctcgt tgccgattat gcccctgtcc acggcctcgc cgatgcccat
     7381 ctccacctcg accccgtcgg tgttggggac cagcctcacc ttccggtcct ttatcccccc
     7441 gtatcggggc caggacttgt cctccgggtc cacgaacagc tcggcctcca tcatgttgaa
     7501 ctccctgagc cggatcaccc cc
//
LOCUS       DAOSYN010000050         7409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_8501_length_7409_cov_4.219200, whole genome shotgun sequence.
ACCESSION   DAOSYN010000050 DAOSYN010000000
VERSION     DAOSYN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8501_length_7409_cov_4.219200"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLJ11_03335"
     CDS             complement(<1..848)
                     /locus_tag="PLJ11_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03733.1"
                     /translation="MATPITVGERIVLHLAQYSKHMNDFDAPFEVSQDGIGEALRISR
                     AHAAIELKKLKEAGTVTERIAHIRAGPVKRKVYFLTEAGEAKARDLKDFANRQGIELS
                     PLLDLRKCKGEELWAQLNPDSRALLAKAVVFRKPFKRSALPQTTISLLPEDKEGMVDM
                     PPTLRESIPRLMDRGALREAHSFAADYWLEEGDYVERLHHLLQANRTLEAQMLLSNRG
                     WQMALTGGPELLASLKELDHVLPRYAREIRRVKGETALRNRDAAYGLLVAEEMLASGD
                     ADERLEG"
     gene            997..1827
                     /locus_tag="PLJ11_03340"
     CDS             997..1827
                     /locus_tag="PLJ11_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOO03734.1"
                     /translation="MRLLVASAPDPASLNIRDRLLEMGEWRECGTFRGQRCYRLRDML
                     MVSFDDLHLHLDHVDRLVGEALEVEIGEVVFLSKHRAASGRPTLTVHPIGNFGRAEYG
                     GRDGTLVPASPAFMSGLLRALSVSAKGLPFEVSYEVTHHGPYLEMPTTFVEIGSDEGG
                     WGNQEAGKAVTRALLGWVPADGPTVVGVGGGHYAPRFTEVTLAKRVRFGHMVAKHVLD
                     GKGDNEVLEIVGKAAGASGTSAVYIHRKSFSRPEARRLGELFEGAGLRVLEGADLQDL
                     "
     gene            complement(1824..2477)
                     /locus_tag="PLJ11_03345"
     CDS             complement(1824..2477)
                     /locus_tag="PLJ11_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO03735.1"
                     /translation="MPQVQHDAILAGGALTIIGGLLMLMSAFMPWVSMQDDGQWTLWA
                     LDLLQRELLYTVIPAASTIGAALVTTASLVLYRVFESHRPLKALPIIQALMAMASSLL
                     AIASIILLNQEYQGTGALYGAGAFTSVVGAILVMAGALLTHAMSGRRPKASPTGFQAL
                     AARSKGPVGRREWKPPETAVKAPRCPSCGEELRPGWKACPNCGYALISEEREGKGSL"
     gene            complement(2593..3276)
                     /gene="pyrH"
                     /locus_tag="PLJ11_03350"
     CDS             complement(2593..3276)
                     /gene="pyrH"
                     /locus_tag="PLJ11_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOO03736.1"
                     /translation="MDKVVVSLGGSVIVPDDDDDIFLKRFAEMVWSLCDRYQLYLVCG
                     GGRIARYYIKVGRALGLQESDLDELGIMTTRINATLVARVLGERSIGKVPTDILEAHR
                     MERKGKVVVMGGTVPGHTTDAVSAMLAKEVKAVRIVNGTSVDAVYTADPRKDPKAERL
                     PRLTHQQLYELVNVGLHGAGPSNVFDRLGAQIARDNHIDIYIVDGRDLADMRAAIEGR
                     PIKGTVVSD"
     gene            3340..4587
                     /gene="prf1"
                     /locus_tag="PLJ11_03355"
     CDS             3340..4587
                     /gene="prf1"
                     /locus_tag="PLJ11_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HOO03737.1"
                     /translation="MDEVAGETLSDRQKYDFKRALEEIKELKGRGTELISVYVPPNKQ
                     ISDVAAYLREELSQSSNIKSQGTRKNVQGAIQSILARLKYFKAPPANGVIFFVGEVST
                     TGDQTRMVQYVLEPPEPITTFVYRCDSDFYLEPLLDMLVDKKVFGLLVIDRSEATIGI
                     LRGRRISVVRNLPSLVPSKHRMGGQSAQRFERLIEISANEYYKKVSDIMTETFLNMEG
                     LQGILIGGPGPTKDYFVKEGYLHHELAKKIIDTFDTGYTDEYGLKELVEKAKDKLYDL
                     DLMREKRYIQQFLNEVRKTEGGLSVYGEEQIRQAIKNGAVDTLLVSEGLRKRRVTLDC
                     PNCGTLEVTLDKSPEGLMMACPKCGTAGTVTKDIDLVDDLFDRAGLMGTKVELISPES
                     EEGEMLLKAFGGMAGLLRFSIGV"
     gene            4588..6300
                     /gene="argS"
                     /locus_tag="PLJ11_03360"
     CDS             4588..6300
                     /gene="argS"
                     /locus_tag="PLJ11_03360"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOO03738.1"
                     /translation="MDALGHFEEHIRAKVAAELAAMGFSGEIALERPDHELADLALPC
                     FQFAKRLRKAPSAIAEELSARIHPDERIGKVWADKGYINFRVNEAVLSALVIKDALER
                     REDFGRGDPRPGKVLLEHTSVNPTGPIHVGRARNPLIGDTLARCLRMCGHEVSTEYYV
                     NDVGKQVVLLTWALEHLTEKDVPPSDREKTDHRLVGYYQAANRLMEASEEVADAVAAM
                     TQRFEAGDLEVIAKVRRTVDLMLDGIKESLSGADVHLDRYIYESQFILDGSAHRVVER
                     LKASPYAAIEDGAYYLNLEGFGVHGRDTRFFFTRKDGTTLYTTRDLAYHLDKFKRADE
                     VLNILGQDQKLGQKQLACALRILGCDRAPECIFYSFVSLPEGRMSTRKGVVVYLDDLL
                     EEAVDRAYQEVCSRRPDLDEERKRRIARDIGVGAVRYNIIRVQAEKQLVFKWEEALNF
                     EGNSAPFVQYAHARCCSILRKAGDFNKSADVSVLTDPYERRLIWTLAQFQEVVRQAGD
                     KRRINMLPTYGHELASALNQFYGAVPVLRSEHQDARLCLVEATRWALRNALVTMGIAA
                     PEEM"
     gene            6300..6791
                     /locus_tag="PLJ11_03365"
     CDS             6300..6791
                     /locus_tag="PLJ11_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO03739.1"
                     /translation="MVEIRKLKDEDKPQVRTLELFCVREYLEGSLRKNWDDLPAELVE
                     QLGASAKDSFEFYRDGGMSYVAVEQGQVIGFLFAQIIRYVNNIEKVLWIENIGVHPEH
                     RRKGVGLKLLRRAALDAKKKGAKAVQSVIMPDSKEAVMLHKKVGFFMDGRKFAFLDLE
                     NFQ"
     gene            complement(6792..>7409)
                     /locus_tag="PLJ11_03370"
     CDS             complement(6792..>7409)
                     /locus_tag="PLJ11_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOO03740.1"
                     /translation="RDRPTISLGYFEKARAVVDEEALTSGEVFLVRRMSGGSTIYTDQ
                     GQLIFSLTVDQGAVPRPEDAYALTCTVLVRALRALGVPAEHKPPNDVVVNGRKISGSA
                     QTRKRGMLMVHGTVLVDTDLDRMVRVLRPRQDKRSRTREQLTCLREELGRPVDIGEVK
                     EAVVLAFSEGLEQRPVRIPVNEREKGRVKALIEEKYGKEGYILQF"
BASE COUNT         1378 a         2242 c         2414 g         1375 t
ORIGIN      
        1 ccctccaggc gttcgtccgc gtccccggac gccagcatct cctccgccac gaggaggccg
       61 tacgccgcgt ccctgttgcg cagggccgtc tcccccttga cccttcttat ctcccgggcg
      121 tagcgcggga gcacgtggtc cagctccttc aggctggcca ggagctccgg gccgccggtc
      181 aacgccatct gccacccccg attggagagc agcatctggg cctccagggt gcggttcgcc
      241 tgtaacaggt gatgtagcct ttccacatag tccccttcct ccaaccagta atcggcagcg
      301 aagctgtgcg cctccctcaa ggcgcccctg tccatgagcc gggggatgga ctcgcgcagg
      361 gtggggggca tgtccaccat gccctccttg tcctccggaa ggaggctgat cgtcgtctgt
      421 ggaagcgcgg agcgtttgaa cggtttacgg aagaccacag ccttggccaa gagggcgcgg
      481 gaatccggat tgagctgggc ccagagctcc tcccccttgc atttcctcag gtccagcagg
      541 ggcgagagct cgatgccttg cctgttggcg aagtccttca ggtccctggc cttggcctcc
      601 cctgcctcgg tgaggaagta gaccttgcgc ttgaccggcc ctgccctgat atgggctatc
      661 cgctcggtca ccgttcctgc ctccttgagc ttcttcagtt cgatggcggc gtgcgcccgg
      721 gagatgcgga gcgcttcgcc gatgccgtcc tggcttacct cgaacggggc atcgaaatcg
      781 ttcatatgct ttgagtattg ggcaagatgg agtacgatcc tttcccctac agtgatcggc
      841 gttgccattg caatcctgat caatgatgat cgtatgaatt aaactgaatt tggttacatg
      901 tagcattttt gataatcatg aaggccgatg atcgggaagg ctggcggatc agaaggacgc
      961 cataaatgta ttaatcccgt aacttatcgg cggcctatgc gcctgttggt cgccagcgcc
     1021 cctgaccctg cgagcctcaa catcagggat cgtctcctgg agatggggga atggcgggaa
     1081 tgcggcacct ttcgggggca gcggtgctac cggctgcggg atatgctcat ggtgtccttc
     1141 gatgacctcc atctgcacct ggaccacgtg gaccggctgg tgggagaggc gcttgaggtg
     1201 gagatagggg aagtggtctt cctgtccaag catcgggccg cctccggaag gccgaccctg
     1261 accgtacatc ccatcggcaa cttcgggaga gccgaatatg gcggtaggga cgggacgctc
     1321 gtcccagcct cgccggcctt catgagcgga ttgctgaggg cgctttcggt aagcgcaaag
     1381 ggcctaccct tcgaggtgtc ctatgaggtc acgcatcacg gtccctacct ggagatgcca
     1441 acgaccttcg tggagatcgg cagtgacgag gggggatggg gcaaccagga ggccggaaag
     1501 gcggtgaccc gcgcccttct gggatgggtg cctgcggacg ggccgacggt ggtgggcgtg
     1561 ggcgggggcc attacgctcc ccgcttcacg gaggtcaccc tcgccaagag ggtgcgcttc
     1621 ggtcacatgg tggccaagca tgtcctcgat ggaaaggggg acaacgaggt gttggagatc
     1681 gtgggcaagg ccgcgggcgc aagcgggacc tccgccgtct acatccacag gaaatcgttc
     1741 tcccggccag aggcgaggcg gcttggcgaa ctgttcgaag gggcggggct cagggtgctc
     1801 gagggggctg acctgcagga cctctagagc gaccccttgc cctccctctc ctcgctgatg
     1861 agggcatagc cgcagttggg gcaggccttc cagcctggcc tcagctcctc tccgcaggag
     1921 gggcacctcg gcgccttcac cgcggtctca ggaggcttcc attcccttct gcccacgggc
     1981 cccttgcttc gggccgccaa tgcctggaag ccggtggggg aggccttggg cctgcgtccg
     2041 gacatggcat gcgtcaatag ggccccggcc atgaccagga tggcgccgac cacgctggtg
     2101 aacgctccgg ccccgtacag cgcaccggtc ccttggtatt cctggttcag caggatgatc
     2161 gaggcaatgg cgagcaggga actggccatt gccatgagcg cctggatgat cggaagcgcc
     2221 ttcagtggac ggtgcgactc aaacacccga tacaggacca gggaggccgt ggtcacaagc
     2281 gcggccccga tggtggaggc ggctgggatg accgtataaa gaagctcgcg ctggagcagg
     2341 tcgagggccc atagcgtcca ttggccatcg tcctgcatgc tgacccaggg catgaaggcg
     2401 gacatcagca tgagcaggcc tccgatgatc gtgagcgctc cccccgccaa tatggcgtcg
     2461 tgctgcacct gcggcatgct ggagcgcggc ctcgggggct gcggggccgg cggggctgcc
     2521 cgccctttct ttttaggagc cccctccctc cgcccgccct tcgcccttcc ctgtccttca
     2581 tttgagaatg ggttaatctg aaaccaccgt ccctttgatg ggcctcccct ctatggccgc
     2641 cctcatatcg gcgaggtccc tcccgtcaac gatgtagata tcaatatggt tatcgcgggc
     2701 gatctgcgct cccagcctgt cgaagacgtt ggatggtcct gccccgtgca gccctacgtt
     2761 caccagctcg tacagctgtt ggtgcgtgag ccttggcagg cgctccgcct tgggatcctt
     2821 gcgcgggtca gccgtgtaga cggcgtccac ggaggtgccg ttgacgatgc ggacggcctt
     2881 gacctccttc gccagcatgg ccgagacagc gtcggtggtg tgcccgggaa cggtgccccc
     2941 catgaccacc accttgccct tcctctccat gcgatgcgcc tccaggatgt cggttgggac
     3001 cttgccgatg ctcctctccc ccaggacgcg agcgacgagc gtggcgttga tgcgggtggt
     3061 cattatgccc agctcgtcca gatcgctctc ctgcaggcct agcgccctgc cgaccttgat
     3121 gtagtagcgg gctatcctcc ctcccccgca caccaggtag agctgatagc ggtcgcacag
     3181 ggaccagacc atctccgcga acctcttcag gaagatatca tcgtcatcgt ctggcactat
     3241 cactgagcca ccgagggaga ccactacctt gtccatttgc ataccaacag atataagtga
     3301 gaaccgtttt cacctaaaaa aaggataggt cgttggatca tggatgaagt agcaggcgag
     3361 accctctccg atcgccagaa gtacgatttc aagagggcct tggaggagat taaggaactc
     3421 aagggccggg gcaccgagct catatccgta tacgtcccgc ccaacaagca gatctccgac
     3481 gtcgccgctt acctgaggga ggagctctcc caatcctcca acatcaagtc ccagggcacc
     3541 aggaagaacg tccagggggc tatccaatcc atcttggcga ggctgaagta cttcaaggcg
     3601 cccccggcga acggggtcat cttcttcgtg ggcgaggtct cgaccacggg ggaccagacg
     3661 aggatggtgc agtatgtgct cgagccgccg gagcccatca ccaccttcgt gtaccgatgc
     3721 gactcggact tctaccttga accgctgctg gacatgttgg tggacaagaa ggtgttcggc
     3781 ctgctggtca tcgaccgcag cgaggccacc ataggcatcc tgcgggggag gcgcatctcg
     3841 gtggtccgga accttccctc gctcgtccct tccaagcata ggatgggggg gcagtccgcc
     3901 cagcggttcg aacggctgat cgagatctcc gccaacgagt actacaagaa ggtcagcgac
     3961 atcatgacgg agacgttcct caacatggag gggctgcagg gcatcctgat cggggggcct
     4021 gggcctacga aggactactt cgtgaaggag ggatacctgc atcacgagct ggccaagaag
     4081 atcatcgata ccttcgacac cgggtatacg gacgagtacg ggctcaagga gctggtcgag
     4141 aaggccaagg acaagctcta cgacctggac ctcatgaggg agaagcgcta catacagcag
     4201 ttcctgaatg aggtccggaa gacggagggc gggctttccg tctacgggga ggagcagatc
     4261 cgccaggcca taaagaacgg ggcggtggat accctcctgg tctcggaagg cctgcggaag
     4321 cgaagggtga ccctcgattg ccccaactgc gggacgttgg aggtcaccct tgacaagagc
     4381 ccggaagggc tgatgatggc ctgcccgaag tgcgggaccg ccggcacggt caccaaggac
     4441 atcgacctcg tggacgacct gttcgaccgg gcgggcctca tgggcacgaa ggtcgagctc
     4501 atctccccgg agtcggagga gggagagatg ctcctcaagg cgttcggcgg gatggccggg
     4561 ctgctcaggt tcagcatagg tgtgtaaatg gacgccctag gtcatttcga ggagcacatc
     4621 agggccaagg tggccgccga gctggccgcc atggggttca gcggggagat cgccctggag
     4681 aggccggacc atgagctggc cgaccttgcc cttccttgct tccaattcgc caagcggttg
     4741 aggaaggcgc cctcggccat cgccgaggag ctgagcgcca ggatccaccc ggacgagcgc
     4801 atcggaaagg tctgggcgga caagggctat atcaatttca gggtgaacga ggcggtgctc
     4861 agcgccctgg tcatcaagga tgccttagag cgccgggagg acttcgggag gggcgatccg
     4921 cgcccaggga aggtgctctt ggagcataca tccgtcaatc ccacggggcc catccacgtt
     4981 ggcagggcga ggaaccccct gatcggggac accctggcga ggtgcctgcg catgtgcggc
     5041 catgaggtct cgacggagta ttacgtgaac gacgtcggga agcaggtggt gctgctgacg
     5101 tgggccctgg agcatctgac cgagaaggac gtcccgccct cggaccgcga gaagaccgac
     5161 catcggctcg tcggctatta ccaagcggcc aacaggctga tggaagcctc cgaggaggtg
     5221 gccgatgcgg tcgcggcgat gacgcagcgg ttcgaggcgg gggacctgga ggtcatcgcc
     5281 aaggtgcgcc ggacggtcga tctgatgcta gacggcatca aggagagcct gagcggggcg
     5341 gacgtgcacc tggaccgcta catctatgag tcccaattca tcctggacgg cagcgcccac
     5401 cgggtggtgg agaggctcaa ggcctccccc tatgcggcga tcgaggacgg cgcctattac
     5461 ctgaacctgg aggggttcgg cgtgcacggg cgggacaccc gcttcttctt cacccggaag
     5521 gatggcacga ccctctacac cactcgggac ctggcgtacc atctggacaa gttcaaacgg
     5581 gcggatgagg tcctcaacat tctggggcag gaccaaaagc tggggcaaaa gcagctcgcc
     5641 tgcgccctga ggatcctggg atgcgatcgg gccccggagt gcatcttcta ttcgttcgtc
     5701 tcccttccag aggggcgtat gtccacgcgg aagggcgtcg tggtctatct ggatgatctg
     5761 ctggaggagg cggtggaccg ggcctaccag gaggtctgct cccgccgccc cgacctcgac
     5821 gaggaacgga agcgcaggat cgcccgggac attggcgtgg gggcggtccg atacaacatc
     5881 atcagggtac aggcggagaa gcagctggtg ttcaagtggg aggaggccct gaactttgag
     5941 ggcaacagcg cccccttcgt tcagtacgcg catgcccgat gctgctccat cctgcggaaa
     6001 gcgggagatt ttaataaaag cgctgacgta tctgtgctca ccgacccata cgagcgtagg
     6061 ctcatatgga ccttggcgca gttccaggaa gtggttcggc aggcggggga taagcggcgc
     6121 atcaacatgc tccccacata cgggcatgag ctggcctctg ccctgaacca gttctatggc
     6181 gccgttccgg tgctcaggtc agagcaccag gacgccaggc tctgcctggt ggaggcgacc
     6241 cgttgggcgc tgcgaaacgc attggtgacc atgggaatag ccgctccgga ggagatgtga
     6301 tggtggagat aaggaagctc aaggacgagg acaaaccgca ggtgaggacg ctggagctgt
     6361 tctgcgtccg ggagtatctg gaaggatcgc tgcggaagaa ctgggatgac ctaccggcgg
     6421 agctcgtgga gcagttgggg gcctctgcca aggactcctt cgaattctac cgggacgggg
     6481 ggatgagcta tgtggccgtg gagcaggggc aggtgatagg ctttcttttc gcccagatca
     6541 tccgctatgt caacaacatc gagaaggtcc tctggatcga gaacatcggc gtccatccgg
     6601 agcaccggcg caagggggtg ggcctcaagc tgctccggcg cgctgccctg gacgctaaga
     6661 agaagggcgc caaggccgtg cagagcgtga tcatgccgga cagcaaggag gcggtcatgc
     6721 tgcacaagaa ggtggggttc ttcatggacg ggaggaagtt cgcgttcctg gacctggaga
     6781 actttcagtg atcagaactg gaggatatac ccctccttcc cgtacttctc ctcaataagg
     6841 gcctttaccc gccccttctc cctttcattg acagggatgc ggaccggcct ctgctccaat
     6901 ccctcggaga aggccagcac gaccgcttcc ttcacctcgc cgatgtccac tggccgtccg
     6961 agttcttctc tcaggcaggt cagctgctcc ctcgtcctgg aacgcttgtc ctgccggggc
     7021 cgcagcacgc ggaccatgcg gtccaggtcg gtgtccacaa ggactgtgcc gtgcaccatg
     7081 agcatccctc gcttgcgcgt ttgggcgctt ccggagatct tcctgccgtt caccaccaca
     7141 tcgttgggcg gcttgtgctc ggcaggtacc cccaaggccc tcagcgccct gaccagcacg
     7201 gtgcaggtga gcgcataggc gtcctccggt cggggcaccg cgccctggtc cacggtgagg
     7261 gagaagatca gctgcccctg gtcggtgtag atggtgctcc cgccggacat cctgcggacg
     7321 aggaagacct cgccgctcgt cagcgcctcc tcgtccacga ccgccctcgc cttctcgaag
     7381 tagccgaggg agatagtggg cctgtcccg
//
LOCUS       DAOSYN010000051         7396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_8525_length_7396_cov_4.414385, whole genome shotgun sequence.
ACCESSION   DAOSYN010000051 DAOSYN010000000
VERSION     DAOSYN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8525_length_7396_cov_4.414385"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..176
                     /locus_tag="PLJ11_03375"
     tRNA            104..176
                     /locus_tag="PLJ11_03375"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:138..140,aa:Gln,seq:ctg)
     gene            complement(186..764)
                     /locus_tag="PLJ11_03380"
     CDS             complement(186..764)
                     /locus_tag="PLJ11_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOO03741.1"
                     /translation="MDRLDEMLLELKDTSELMIDLAYSSLLYNNKEIAEEVVLMEEML
                     DELANDIQRRAIVRAMDDRDEGKAFAIIKLAVAVEEISDAAMQIATVTIQEDEPHPVI
                     RLSIKDADTTITRAEIKPGSDLVGKSLGKMRLASQSGMWVVAVKRERKYLYGPDKNVV
                     LQVGDVVIARGPPEAEQYFQDICDGKERLEEP"
     gene            complement(764..1408)
                     /locus_tag="PLJ11_03385"
     CDS             complement(764..1408)
                     /locus_tag="PLJ11_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOO03742.1"
                     /translation="MIDIGREKRLDKYDLAHATVREILTEMKDISEIMVDLAYASIMF
                     DSEEIAEEVKNLEEDMEELSKNIRIKAMLAARTHKEAAKLSSLLEVASASRRISNAAG
                     DIVKIMEVDVAKRPFLGFILREAEEKIKRLSLAEGSDMVGRLIGELGVESETGMRIIA
                     IKRGKRWIYDPEEDTKLKAGDVLIVRGTEDGYEVLRSYANGEERWPVYPEEEED"
     gene            1677..2909
                     /locus_tag="PLJ11_03390"
     CDS             1677..2909
                     /locus_tag="PLJ11_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOO03743.1"
                     /translation="MDGAVKAFFSNNRTVFTMGFTALLISSLGDILAGITLGFMTDTL
                     EMLPGLMVLIPAAIGMRGNIFGALGSRLGTAMHVGTFELSLRKGSILRQNIEASLIMT
                     MVVSFLMGFLAKVISTAIGMESISMQQFIFISVLGGVLAGLVLLVVNVIVATLGFRRN
                     WDIDNISAPLLTAAGDIVTLPMLFLAGLIVIKGEGVIDAAFYDVMTLLFLAITIFLIY
                     EAMCKRDEETKRIFVQSSPVLVICILLDLGAGLTIEDQLSKLVAYPALLVLIPLFLQN
                     CNALGGILTSRLSSLLHMGIMLPGKTPGKGSYENFAIIYIFALWVFAFVSMATHFVCL
                     ALGLASPGLLAMVTLGLVAGLITITVLNFLAYYVAVTTFMLSLDPDDHSIPVTSSAID
                     FIGSMALMAIIVAMGLSV"
     gene            2966..4123
                     /locus_tag="PLJ11_03395"
     CDS             2966..4123
                     /locus_tag="PLJ11_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase family protein"
                     /protein_id="HOO03744.1"
                     /translation="MIDRENILGILESYDPRKVKIGVLASHSALDVCDGAVEEGFRTL
                     AVCQEGRDSPYTKYFKAFRDRNGKAMRGMVDETLMLKRFSEATTPAVMDRLLKDNVLF
                     VPNRSFTSYCGIDAVENDFRVPMVGSRSLLRCEDRGGERDYYWLLEQAGLPSPRKVER
                     PEDIDALTIVKLHHKQKKLERGFFTASTPEEYWEKANKLIRDGVIEEDSLADARMEEY
                     IIGPVFNLDFFCSPLEEKGSRIELLGVDWRFESSLDGFVRLPAEQQLALNDRQRIPEY
                     TVCGHNSATMRESLLEGAFELAERFVSAAERHFFPGMIGPFCLQTCVDKDLRFFIYDV
                     APRIGGGTNVHMAVGHPYGNSLWRRSMSTGRRLAMEVRRAIEEDRLGEIVT"
     gene            4274..7183
                     /locus_tag="PLJ11_03400"
     CDS             4274..7183
                     /locus_tag="PLJ11_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO03745.1"
                     /translation="MQRNRTWKLATGLMVLMLLATPFLAFVPSEVSAVTTGNKLSIIV
                     YDEGNGNPVVGATVTLIGVHQQSAIPSKTTVSGGLAEFTPGDGYYRLVVSKEGYYNYE
                     SRDESGEIIRFTDLEAKTIFAPMEKMSEDKYTLSLQVNDTSGGLSGADVKIKTVRDNI
                     TTTISRKADSTGVVTLQLPNGTYSVVVSKLNYTTRVQDVTINGMDISETITLSPSVRY
                     SGFVTVAGAPATGVVAYLVCSNSFADKEKIIKASGVSPYFSFDAYVGNFTLLVDADGA
                     LAKVENVTINATNPVKTIALSKQSVQTETNHFEFGDEDWNTAMLTKSLVLDHDNTVPG
                     LPYAELPSARMQIDLVFGDGDGAVNESEAAAFEAKMEAFGPIDVTTIGMLAVGAQSLV
                     SADADLEYTITSMEGPVTSNAQFRVEMVAGYTTIEPVANGLSSYTVKVNALKTSANMS
                     YTYALAFPDLYEKVGSATISPASSGATVSGYTTVTIASGAAAQAEVTLTIQSSVAPTA
                     AAAIVTGADAYKVLNGSTLLHYIVAQDANVTFTASGSSDPNGNPLIYIWNFGDGNSSG
                     EVSNVTYVFAYADAKQYTVNLTVKDQAGLVAYKAFTVKVDGVPPTVVAKENVTVLGST
                     LNVDQNKAYTFNSADSYDRLNSSEEAGLIASYVWDWGDGNSTTVLMGENQTVTHTWTK
                     AGTFQMYLNVTDVANHTTSKPVAVSVRDTVPPTVKFSVKLNDTVVTSAKENQTLTFDA
                     SASSDASGIASYLWEFGDGTNSTLASPTHKYQAIKTFTVKLTLTDSAGNSANTTYSLK
                     VESSARPDLRVGTVDFEPDRFTEGEAGYIYVNITNVGTARAEGIYAQLWKLNLDGTRT
                     GLTDESVLLVNGTEATYLEVGETGVLRFKHTFGSKGDYTLQVNVSASNEVSSKLTDNS
                     ATVSLTVEEAGWKTWLLYGGIFAVIIVVVVLFLFRKKLPKISGKKKAPAPPAKRK"
BASE COUNT         1461 a         2381 c         2118 g         1436 t
ORIGIN      
        1 cgatcgacga cgtctacaag cgcatcgtgg cggccatcaa ggccgcctga gccggcccct
       61 gcgaaaaggt taaaggcagg gagcatttac caccgttccg cacagccccg tagtgtagcg
      121 gtcaatcatg cgggactctg gatcctgcga cggcagttcg aatctgcccg gggctactcc
      181 ttttcctacg gctcctccag gcgctccttg ccatcacaga tgtcctggaa gtactgctcg
      241 gcctctggcg ggccacgggc tatcaccaca tcgccgacct ggagcacgac gttcttgtcc
      301 gggccgtaga ggtacttgcg ctccctcttc accgccacca cccacatccc gctctggctg
      361 gccagccgca tcttccccag gctctttccc acaaggtccg agcccggctt gatctccgcc
      421 cgggtgatcg tcgtatccgc gtccttgatg ctcagcctga tgaccggatg cggctcgtcc
      481 tcttggatcg tgaccgtggc tatctgcatc gccgcgtccg atatctcctc caccgccacc
      541 gccagcttga tgatcgcgaa ggccttgccc tcgtcccggt cgtccatcgc ccggacgatc
      601 gccctgcgct ggatgtcgtt tgccagctcg tccagcatct cctccatcag cacgacctcc
      661 tcggcgatct ccttgttgtt gtaaaggagc gaggagtagg ccaggtcgat catgagctcg
      721 gacgtgtcct tgagctccaa tagcatctcg tcaagccggt ccatcagtcc tcctcctctt
      781 cggggtacac cggccatctc tcctccccgt tcgcatatga gcgcaggacc tcgtacccgt
      841 cctccgtgcc gcgcacgatc aggacatccc ccgccttcag cttcgtgtcc tcctccgggt
      901 cgtagatcca gcgcttgccc cgtttgatgg cgatgatgcg catccctgtc tcggactcca
      961 cgcccagttc acctataagc cgccctacca tgtccgaccc ctccgccagg gagaggcgct
     1021 tgatcttctc ctccgcctcc ctgaggatga agcctaggaa gggccgcttg gccacgtcca
     1081 cctccattat cttgactatg tccccggccg cgttggagat gcggcgggag gccgaagcca
     1141 cctccaacag ggacgacagc ttggccgcct ccttgtgggt acgggcggcc agcatggcct
     1201 ttatcctgat gttcttgctg agctcctcca tgtcctcctc gaggttcttg acctcctcgg
     1261 cgatctcctc gctgtcgaac atgatggagg cgtaggcgag gtcgaccatg atctccgaga
     1321 tgtccttcat ctcggtcagg atctccctga ccgtggcatg ggccaggtcg tacttgtcaa
     1381 gacgtttctc ccttccgatg tcaatcataa aaggaacacc tgcgccacgt cacagcgacc
     1441 gacctgcagc taccgcaagg gttgggcaag ggcattgctc gtgacatctc cattggcctc
     1501 atagggccat cggcctcgaa taggcctggg cccgccgttc cttccttatg aggttttcca
     1561 tatatcatcg ttgcgaattc gccagcccgt tccatccccg caagcacggc caggcatccc
     1621 tgatggaatg acttattatt aatagggtca aacgctacct ctaggcgggt ggtacgatgg
     1681 acggggcagt gaaggcgttc ttctccaaca acaggacggt cttcaccatg gggttcacgg
     1741 cccttctcat ctcgtccctc ggggacatcc tggccgggat caccctgggc ttcatgaccg
     1801 acaccctcga gatgctgccg gggctgatgg tcctcatccc ggcggccata gggatgcggg
     1861 gcaacatctt cggcgccctg ggaagccgcc tgggcacggc catgcacgtg ggcacgttcg
     1921 agctctccct gcgcaagggg agcatcctgc ggcagaacat cgaggcctcc ctgatcatga
     1981 cgatggtggt ctccttcctg atgggcttcc tggccaaggt gatctccacc gccatcggca
     2041 tggagagcat cagcatgcag cagttcatct tcatctccgt cctcgggggg gtgctcgccg
     2101 ggctcgtcct cctggtggtg aacgtgatcg tcgccacgct gggcttccgg aggaattggg
     2161 acatcgacaa catcagcgcg cccctattga ccgccgcagg ggatatagtg accttaccga
     2221 tgctcttcct ggccggcctg atcgtcataa agggcgaggg ggtcatcgac gccgcgttct
     2281 acgatgtcat gacgctcttg ttcctggcga tcaccatctt cctgatctac gaggccatgt
     2341 gcaagaggga cgaggagacc aagcgcatct tcgtgcaaag ctccccggtg ctggtgatct
     2401 gcatcctgct cgacctgggg gcagggctga cgatagagga ccagctcagc aagctggtcg
     2461 catacccagc gctgctggtg ctcatcccac tgttcctgca gaactgcaac gccctgggcg
     2521 gcatcctgac gtcccggctg tcctctctgc tgcacatggg catcatgctc ccagggaaga
     2581 cgccgggaaa ggggagctat gagaacttcg ccatcatcta catcttcgcc ctctgggtct
     2641 tcgccttcgt cagcatggcc acccacttcg tctgcctggc actggggctg gcctctcccg
     2701 gattgctcgc catggtcaca ttgggcctgg tggccggcct gataaccatc accgtgctca
     2761 acttcctagc gtattacgtg gcggtcacca ccttcatgct ctccttggac cccgatgacc
     2821 acagcatccc ggtcacatcc tcggcgatcg acttcatagg gtccatggcc ctcatggcca
     2881 tcatcgtggc catgggcctc tcggtctgag ttggcacaac ttaatatgct atggaacgtt
     2941 ccctatccat tcgcgaggga ccctaatgat cgacagggaa aatatccttg ggatactgga
     3001 atcctacgac ccgaggaagg tgaagatcgg cgtgctggcc tcccactccg ctctggacgt
     3061 gtgcgatggg gcggtggagg aaggcttccg caccctggcc gtatgccagg agggccggga
     3121 cagcccctac acgaagtatt tcaaggcgtt ccgggaccgg aacgggaagg cgatgagggg
     3181 aatggtggac gagacgctga tgctgaagag gttcagcgag gccaccaccc ccgcggtcat
     3241 ggaccggctg ctcaaggata acgtcctctt cgtccccaat cgctccttca cctcctattg
     3301 cggcatcgat gcggtggaga acgacttccg tgtcccgatg gtcggttccc gctccctcct
     3361 gcgctgtgag gaccgagggg gggagaggga ctactattgg ctcttagaac aggcggggct
     3421 gccgtcaccg cgtaaggtcg agcgtccgga ggacatagac gccctcacga tagtcaagct
     3481 gcatcacaag cagaagaagc tggagcgcgg cttcttcacc gcctccaccc cggaggagta
     3541 ttgggagaag gcgaacaagc tcatccgtga cggcgtaatc gaggaggatt ctttggcgga
     3601 cgcccggatg gaggagtaca tcatcggccc ggtgttcaac ctggactttt tctgctcccc
     3661 cctggaggag aaaggctccc ggatcgagct cctcggggtc gattggcgct tcgaatcgtc
     3721 cttggacggc ttcgtccggc tgccggcgga gcagcagctg gcgctcaacg accgccagcg
     3781 catccccgag tacacggtct gcgggcataa ctcggccacg atgcgcgagt cgctgttgga
     3841 gggggcattc gaactggccg agaggttcgt cagcgccgcg gaaaggcatt tcttcccggg
     3901 gatgatcggc cccttctgcc tgcagacctg cgtcgacaag gacctgaggt tcttcatcta
     3961 cgacgtcgcg ccccgcatcg gcgggggcac gaacgtgcac atggccgtag ggcacccgta
     4021 cggcaactcg ctatggcggc gcagcatgag caccggaagg cgcctcgcca tggaggtaag
     4081 aagggctatt gaagaggaca ggttgggcga gatcgtgacc tgagcgatgg ttcgtcccga
     4141 catttcaatc atctccatgg gtatggccag cataggtttc ccttggataa catttattaa
     4201 ggacaacact attccgacat aacccgagac tcagtcagca ctagatgtgc gcctatcatg
     4261 agggagggaa ataatgcaaa ggaacagaac ctggaagcta gcgaccggat tgatggtcct
     4321 gatgctcctg gccacgccat tcctggcgtt cgtgccgtcg gaggtcagcg ccgttacgac
     4381 cggaaacaaa ctgtcgatta tcgtgtatga tgagggaaat ggtaatcccg ttgtcggagc
     4441 cacggtcaca ctgataggcg tacaccagca gagcgccatc ccctccaaga ccaccgtaag
     4501 cggcggtcta gcggaattca cgcccggaga tggatactat agactagtcg tatcaaagga
     4561 aggatattac aactatgaga gcagggatga gagcggggag ataataaggt tcaccgacct
     4621 tgaggccaag accatctttg caccaatgga aaagatgagc gaggacaagt acacgctctc
     4681 ccttcaggtg aacgacacca gtggtggcct gtccggcgcc gacgtcaaga tcaagaccgt
     4741 ccgggacaat atcaccacga ccatctctag gaaggctgat agcactggag tggttaccct
     4801 ccagttgccc aacggtacct attcggtggt ggtctccaag ctcaattaca caacgcgtgt
     4861 acaagacgtc accatcaacg ggatggacat atcggaaacc atcacgctct ccccctctgt
     4921 aagatactcc ggcttcgtga ccgtggcagg ggccccggcc accggggtcg tcgcctacct
     4981 ggtctgctcc aactccttcg cggacaagga gaagatcatc aaggcctcag gtgtttcgcc
     5041 gtacttcagc ttcgatgcct atgtggggaa cttcaccctc ctggtcgacg ccgatggggc
     5101 tctggctaag gtcgagaacg tgaccatcaa cgccaccaat ccggtgaaga ccatcgccct
     5161 ctccaagcag agcgtgcaga ccgagacgaa ccacttcgag ttcggggacg aggactggaa
     5221 cacggccatg ctgaccaagt ccctggtcct ggaccacgac aacacggtgc ccggcctgcc
     5281 ctacgccgag ttgccaagcg ccaggatgca gattgacctg gtcttcggcg acggggacgg
     5341 ggcggtgaac gaaagcgagg ccgccgcgtt cgaggccaag atggaggcct tcggtcccat
     5401 cgatgtgacg accatcggca tgttggcggt cggagcgcag agcctggtct ccgccgacgc
     5461 cgacctggag tacaccataa ctagcatgga aggccctgtt accagcaacg ctcagttccg
     5521 ggtcgagatg gtggccgggt acactaccat cgagccggtc gccaacggcc tcagctccta
     5581 cacggtgaag gtgaacgccc tgaagacaag cgccaacatg agctatacct atgcgctcgc
     5641 cttccccgat ctctatgaga aggtcggcag cgccaccatc agcccggcga gttccggggc
     5701 gaccgtcagc ggctacacca cggtcacgat agcatccggc gccgccgccc aggccgaggt
     5761 gaccctcacc atacaaagct cagtggcccc cacggccgcc gcggccatag tcaccggggc
     5821 ggacgcctac aaggtcctga acggctctac gctcctgcac tacatcgtag cccaggacgc
     5881 caacgtgacc ttcaccgcct ccggctccag cgaccccaac ggcaatcccc tgatctacat
     5941 ctggaacttc ggggacggga acagcagcgg cgaggtctcc aatgtgacgt acgtattcgc
     6001 ctatgccgat gcaaagcagt acaccgtcaa cctgaccgtg aaggaccagg ccgggcttgt
     6061 agcatataag gccttcacag tgaaggtgga tggtgtgccc ccgaccgtcg tcgccaagga
     6121 gaacgtgacc gtgctcggtt ccaccctcaa cgtcgaccag aacaaggcct atacgttcaa
     6181 cagcgccgac tcctatgacc gcctgaacag ctccgaggag gcgggcctca tcgccagcta
     6241 cgtttgggac tggggcgacg ggaacagcac caccgtgctc atgggcgaga accagaccgt
     6301 cacccacacc tggaccaagg ctgggacctt ccagatgtac ctgaacgtga ccgatgtggc
     6361 caaccacacc accagcaagc cggtcgccgt gagcgtgcgg gacaccgtcc cgcccacggt
     6421 caagttctcg gtcaagctca acgacaccgt ggtcacaagc gccaaggaga accagacgtt
     6481 gacgttcgac gcctcggcct ccagcgacgc cagcgggatc gcgagctacc tctgggagtt
     6541 cggcgatggg accaacagca ccctggcctc cccgacccac aaataccagg ccatcaagac
     6601 gttcaccgtc aagctgacat tgaccgacag tgccggcaac tccgccaaca ccacctacag
     6661 cctgaaggtc gagtcctcgg cccgtccgga cctgcgggtg ggcaccgtgg acttcgagcc
     6721 tgacaggttc accgagggcg aggccggcta catatacgtc aacatcacca acgtgggcac
     6781 cgcgagggcg gagggaatat acgcccagct gtggaagctc aacctcgatg gcacgaggac
     6841 cggcctgacg gacgagagcg tcctcctggt gaacggcacc gaggccacct acctcgaggt
     6901 cggggagacc ggcgtcctgc gcttcaagca caccttcggc tccaagggcg actacaccct
     6961 gcaggtgaac gtcagcgcca gcaacgaggt ctcctccaag ttgacggaca actccgccac
     7021 cgtctccctg acggtggagg aggccggttg gaagacatgg ctgctgtacg gcggcatctt
     7081 cgccgtgatc atcgtggtgg tggtcctgtt cttgttcagg aagaagctgc ccaagatctc
     7141 cggcaagaag aaggcccccg cgcccccggc caagaggaag taggcaaaac cttttccctt
     7201 ctctttcttc ttattccatt gaatgctgcg gctcatcgat tccgggatgt gcgaccctct
     7261 ctattccgtc gccttggagg aggtcatcct ggagtcctgc tccaagggcc tcatgccccc
     7321 cacgctccat ctttatgtcc gggacaggcc caccatttcc ctggctcgtt tcagtctcca
     7381 cccgatatcc tccatg
//
LOCUS       DAOSYN010000052         7342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_8606_length_7342_cov_4.621929, whole genome shotgun sequence.
ACCESSION   DAOSYN010000052 DAOSYN010000000
VERSION     DAOSYN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8606_length_7342_cov_4.621929"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLJ11_03405"
     CDS             complement(<1..405)
                     /locus_tag="PLJ11_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HOO03746.1"
                     /translation="MTLPLEILYIRLRNELEACQQYLPDAFDLSERSLTTFPLKVEVS
                     LDRTPGPVMENGKVTYRYNHRLELIIGREYPFEKPLVIWKTPIFHPNIMMPEDGGHVC
                     IKLLSDWSFNSTLSTFIKGLESLLLSPNGGSPF"
     gene            complement(402..647)
                     /locus_tag="PLJ11_03410"
     CDS             complement(402..647)
                     /locus_tag="PLJ11_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03747.1"
                     /translation="MVVRVRVRNAETGATVDMELELENTVEEIIEGVSAYWEKDPGAY
                     VIRRGRRLLRGQQKVQELDVQPNEELELIPDPEGGNR"
     gene            complement(700..1500)
                     /locus_tag="PLJ11_03415"
     CDS             complement(700..1500)
                     /locus_tag="PLJ11_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOO03748.1"
                     /translation="MGDLLRPGERDDDRYDRTRLIRWVDLERVQRARALVVGAGALGN
                     EAVKDLVLFGFRHVDVLDMDTVVRSNLSRCVLFREGDQRSGRGKAELVAERAMELDPA
                     AEVRGLHGRVQALGPDGLRAYDIVLGCLDNIAARLHLNANSYYASVPYVDGGTEGFRG
                     RVQVVVPPTSPCLQCGMNRSHLKVMERRFSCTGQEAVYYRPNLPAEITTTSAIAAIQV
                     REAVKVLSRRAPIEHVMHYDGLAGTCEVLSLEVDPGCPNHGAGRLSQS"
     gene            1640..3766
                     /gene="ppk1"
                     /locus_tag="PLJ11_03420"
     CDS             1640..3766
                     /gene="ppk1"
                     /locus_tag="PLJ11_03420"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOO03749.1"
                     /translation="MTGRKGDRGGMPGKGVPKAAGTSERFINREISWIRFHKRILEEA
                     KDPSHPLLERAKFLAICGSNLDEFFMVRVSGLKRQLRGGALEAPPDGLTPLEQLEAIN
                     RELVPLLEEHGRLWHEDLLPKLRAEGVAIHRIEDLDGESRRALRAHFERMVFPALTPL
                     ALDLNQPFPFISNLSINLAVIVRHPQRGDRYARVKVPLDLFPRFVPLPGSASARTQGF
                     VLLEDLVADNLDLLFPGMRVKEAHAFRITRDADVEIMTDEAEDLLTAIEESMETRKVG
                     SPIRLEVDKDMPDKLVDLFATKLGLHPLMVFRSHAPLSLVSLWDLMALNRPDLKDVPF
                     LPHVPRELMPEKPLMAAVERRDRLFYHPYDSFMPLVTFLRQAAHDPQVLAIKITLYRI
                     DKRSPIIDALMEARENGKAVAAVVELKARFDEENNIVWARALENAGVHVVYGLLDLKV
                     HAKLLLVVRKKGDSIVRYSHMSSGNYNAQTARIYGDIGYLTADPDIGADVADLFNYLT
                     GYSRKDSYRRLLVAPMNLKREMIARIDRETTRQIEHGDGYLAFKLNGLLDPDIIQALY
                     RASQAGARVDLNVRGLCALRPGVRGLSENIRVNSIVGRFLEHARIYYFRNGGEEEVYL
                     GSADMMPRNLNRRVEVLFPVLDAGYRRALVDEILPLHLRDNVKSREMLPDGTYVRRKA
                     AKGEERVDSQDWLLRNRGRWHREEGN"
     gene            3742..5892
                     /locus_tag="PLJ11_03425"
     CDS             3742..5892
                     /locus_tag="PLJ11_03425"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOO03750.1"
                     /translation="MAQGGGELTVDGPCAYGRWVTSQRLDALLAEVDGVRRGEDIECV
                     HRMRVASRRLRSVLRLFGECFKGKRGKRWRKAVRKVTATLGEARDLDVQLELLEALSR
                     EWKEAVDLEPVIATLRRRRDALQPGIAALMDEISGGGAVQELRAELKGAEWDGDASAL
                     RPYAFAHASVAVEELMAHSHSVPVYEDWQGHHELRIAGKRLRYVLEAFRDAYPDRLEG
                     ELRTLKALQDVVGELHDCDVWLQGLPAMQEEVPQASLAFDRLQETFEARRRILHGELV
                     ETWGALVQERFFSRMLEGLKGHRSGEGAPVVLALLSDVHGNSAALRAVLKDARERGAT
                     AFLNAGDTIGLPRPGEAVRTLDAPEGLSVLGNVDRAVLRSKGGRGTGDRQLEMAAALL
                     GQREWEWLSSLPEELRLDVRGRTLLIVHASPGQEEERLLPSTPEARLREVVEGAGADI
                     IVTGHTHVPMVRACGGALFVNPGSVGRPRDGAEASYALLELPAMRAELHRVAYDAEGV
                     AGELRSMGFKDLPEAIVHGMDPMGTLGSWAEGLHGDRAHAEQVRALALLLFDQTCELH
                     CLSPRDRQLLEMAALVHDVGLRDGTEGHQRRALDIVLKADIPVNADDRLMLACVARYH
                     GRRPPRASDRGFRDLRERDRERVRKLSALLALADGLDRGHAALVNWIEVSVGKEEVLL
                     RIKGKGKLGAEREWGLRKAVQFERAFGRKVRIDG"
     gene            5885..>7342
                     /locus_tag="PLJ11_03430"
     CDS             5885..>7342
                     /locus_tag="PLJ11_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOO03751.1"
                     /translation="MADMKRGKVVAFMDVGTNSVRLLVVRIGPDRSYAVLSEQKEMVR
                     LGERVFRDGLLREEAMERTVSVLKNFCDLSRTYGATELVAKATSATREASNRQELIDR
                     VREATGLELQIISGLEEARLIYLGVSSGHHIGHRTALFVDIGGGSTELALGDQDDHSY
                     LESMKVGAIRLTTMFVRRPEGPVPEEVLGKMRKYVKSSLVRARKEVLQRKVSLAFGSS
                     GTIVNLGEVLLRRGSHRPGVIRRSQLKRLIPYLAGMDLATRRKVPGINPERADIIVAG
                     AVVLDTVMDELRLSEIAVSPRGMRDGMLQDYLSGMEGPPGLRELSVRERSVIQLGRAC
                     LIDEEHARRVTIIALDLFDSSERKGLHVMGRKERELLRHAAFLHDVGDFISFNDHHLH
                     SHYMISHAELLGFSRREVLLMANVAMYHRKRMPKVGMAELRELDGKDQEAVVRLSTLL
                     RMAESLDRSHSNLVQGVRFSKVTKRSARLIVTASGD"
BASE COUNT         1305 a         2197 c         2579 g         1261 t
ORIGIN      
        1 gaaggggctc cctccgttcg gagagagcag aagggactcc aagcctttga tgaaggtgga
       61 gagggtggaa ttgaaggacc aatcggacag gagcttgatg cacacgtgcc cgccgtcctc
      121 gggcatcatg atgttcggat ggaagatggg ggtcttccag atcaccaggg gtttctcgaa
      181 cgggtattcc cggcctatga tgagttccaa ccgatggttg tacctgtacg tcactttccc
      241 gttctccatc accggcccgg gggtgcggtc gagggagacc tccaccttca gcgggaaggt
      301 agtgaggcta cgttcgctga gatcgaaggc gtcgggtagg tactgctgac aggcctccag
      361 ctcgttccgg aggcggatgt aaaggatctc caggggaagg gtcatctgtt cccgccctca
      421 gggtcgggta tcagttccag ctcctcgttc ggctgtacgt ccagttcctg aaccttctgc
      481 tggcctcgca acagacgccg ccctctccgg atgacatagg ccccggggtc cttctcccaa
      541 taggccgaga cgccctcgat gatctcctcc acggtgttct ccagctccag ttccatgtcg
      601 accgtggccc ctgtctctgc gttcctgacc cttaccctta ccaccatggt atcgtcctcg
      661 gcaatccggg ggaaggccgt ataatccttt ggattagaat cagctttgtg ataatctccc
      721 cgccccatgg ttcgggcatc ctgggtccac ctccagggac agcacctcgc atgtgccggc
      781 gagcccgtcg tagtgcatca cgtgctcgat gggggcgcgg cgggacagga ccttcaccgc
      841 ctccctgacc tggatggccg cgatcgcgct cgtggtcgtt atctccgcgg ggaggttcgg
      901 ccgatagtag accgcctcct ggccggtgca gctgaaccga cgctccatga ccttgaggtg
      961 gcttcggttc atgccgcatt gcaggcatgg ggacgttggg gggaccacca cctgcacccg
     1021 tcccctgaag ccttcggtcc ccccgtccac gtacgggacg gaggcgtagt aggaattggc
     1081 gttcaggtgc agacgggcgg ctatgttgtc gaggcatccc aggactatgt cgtaggccct
     1141 cagcccgtcc ggccccagcg cctggaccct gccatgtagg cctctcacct cggcggctgg
     1201 gtccagctcc atcgcccgct cggccacgag ttcggccttg cccctgccgg aacgttggtc
     1261 cccttcccgg aagaggacgc agcggctcag gttggagcgc accacggtgt ccatgtccag
     1321 cacgtccaca tggcggaagc cgaagaggac gaggtccttc accgcctcgt tccccagggc
     1381 cccggcccct accactagcg ctcgagcgcg ctgcacacgc tccaggtcca cccagcggat
     1441 gagcctggta cgatcgtagc ggtcatcatc cctttccccc ggacggagca ggtcacccat
     1501 cggggaaggg acgccttagg gcgtataaaa gggtggacgg ccccggtgcg agccatgata
     1561 attactggcg aagaacttga gaagggagag ctggaccgca tcttcatata ttatagagga
     1621 cggaggcgat agggatgtga tgaccggtcg gaagggcgat agggggggca tgccaggcaa
     1681 aggggtcccc aaggccgcag ggaccagtga gcgtttcatc aaccgagaga tcagctggat
     1741 ccggttccac aagcgcatcc tggaggaggc gaaggacccc agccatcccc tcctggaacg
     1801 ggccaagttc ctggccatat gcggcagcaa cctggacgag ttcttcatgg tccgggtctc
     1861 cgggctcaag aggcagctga gggggggagc gctcgaggcc ccgccggacg gcctcacccc
     1921 cctggagcaa cttgaggcca tcaaccgcga gctcgtcccc ctcctggagg agcacggcag
     1981 gttgtggcac gaggacctgt tgccgaagct gcgggcggaa ggggtcgcca tccatcgcat
     2041 cgaggacctc gacggggaga gcaggcgggc cctgcgcgcc cacttcgagc gtatggtgtt
     2101 cccagccctt acccctctgg ccctggacct gaaccagccc ttcccgttca tttccaacct
     2161 gagcatcaac cttgccgtca tcgtgagaca cccgcaacgg ggcgatcgct acgcccgggt
     2221 caaggtcccc ttggacctgt tcccccggtt cgtccccctg cccggcagcg cttcggcacg
     2281 aacgcagggc ttcgttctac tggaggacct cgtggccgac aatctagacc tcctgttccc
     2341 tgggatgagg gtcaaggagg cgcacgcctt ccgtatcacc agggatgcgg acgtcgagat
     2401 catgaccgat gaggccgagg acctgctgac cgccatcgag gagagcatgg agacccggaa
     2461 ggttggttcc cccatccgtc tggaggtgga caaggacatg cccgacaagc tggtggacct
     2521 cttcgccacc aagctcggtc tccatccgct gatggtcttc cgttcccatg ccccgctctc
     2581 cctggtgagc ctctgggacc tgatggcgct caaccgtccc gatctgaagg atgtgccctt
     2641 cctcccccat gtcccccgcg agctgatgcc ggagaagccc ctgatggccg ctgtagagcg
     2701 ccgggaccgc ctgttctatc atccctacga cagcttcatg cccctggtga ccttcctgag
     2761 gcaggccgcc catgaccccc aggtgctggc gataaagata acgctgtatc ggatcgacaa
     2821 gcggtcgccg atcatcgatg ccctcatgga ggcccgggag aacggcaagg cggtggcggc
     2881 cgtcgtggag ctcaaggccc gcttcgacga ggagaacaac atcgtatggg ccagggccct
     2941 agagaacgcg ggcgtgcacg tggtctatgg gcttctggac ctgaaggtgc acgccaagct
     3001 gctgctcgtc gttcggaaga agggggacag catcgtccgt tacagccaca tgagctctgg
     3061 caactacaac gcccagacgg cccgcatcta cggggacata gggtacctca cggccgaccc
     3121 ggacataggg gcggacgttg cggacctctt caactatctt actggctact cccgtaagga
     3181 ctcctaccgg cggctgctgg tcgcgcccat gaacttgaaa agggagatga tcgcaaggat
     3241 cgacagggag acgaccagac agatcgagca tggcgatggc tatctggcct tcaagctcaa
     3301 cgggctgctt gacccggaca tcatccaggc cttgtacagg gcgtcgcagg ccggggcgag
     3361 ggtggacctc aacgtccgcg ggctctgcgc cctccgcccg ggggtgaggg ggctcagcga
     3421 gaacatcagg gtgaacagca tcgtcggccg gttcctggag cacgcccgca tctactattt
     3481 ccggaacggc ggggaggagg aggtgtatct gggaagcgcg gacatgatgc cccggaacct
     3541 gaatcggagg gtggaggtcc tgttccctgt cctggacgcg ggctaccgga gggcgctcgt
     3601 ggacgagatc ctacccttgc acctgaggga caacgtcaaa tcgcgggaga tgcttccgga
     3661 cggcacctac gtcaggcgga aggccgcaaa gggggaggag cgggtggact cccaggactg
     3721 gctgctcagg aaccgaggcc gttggcacag ggaggagggg aactgaccgt ggacgggccg
     3781 tgcgcctacg ggcgatgggt caccagccag aggctcgatg cccttctggc cgaagtggac
     3841 ggggtgcgca ggggggagga tatcgaatgc gtgcaccgca tgcgggtggc ctcgaggcgg
     3901 ttgcggtccg tgctccgcct gttcggggag tgtttcaagg gaaagagggg caagcgatgg
     3961 cgcaaggcgg tcaggaaggt cacggctacg ctgggggagg cgagggacct ggacgtgcag
     4021 ctcgagctcc tggaagcgct ctcccgggaa tggaaggagg cggtcgacct tgagcctgtc
     4081 atcgcgaccc tccgccggcg cagggacgcg ctccagcctg ggatcgcggc attgatggac
     4141 gagatctccg ggggaggggc cgtccaggaa ctccgggcgg agctgaaggg ggccgaatgg
     4201 gacggggatg cgagcgcgct gcgtccctac gccttcgcgc acgcctcggt cgctgtggag
     4261 gaactgatgg cgcattccca ttccgttccg gtctatgagg actggcaggg acatcacgag
     4321 ctgcgcatcg cggggaagcg cctgcgctac gtccttgagg cgttccggga cgcctaccct
     4381 gatcggctgg agggcgagct taggacgttg aaggcgctac aggacgtcgt cggcgagctg
     4441 cacgactgcg atgtatggct gcaagggctg ccggcgatgc aggaggaggt accgcaggcc
     4501 tccctcgcct tcgatcgcct gcaggagacc tttgaggcga ggaggcggat attgcacggc
     4561 gagctggtgg aaacgtgggg cgccctagtc caggagcgtt tcttctcccg aatgctggag
     4621 ggattaaagg ggcaccgatc gggtgagggc gcccctgttg tcctggcctt gctctccgat
     4681 gtgcatggca actcggccgc gctgagggcg gtgctgaagg acgcgcggga gaggggagcg
     4741 accgccttcc tgaacgcggg ggacacgata gggctcccga ggccggggga ggcggtcagg
     4801 accttggacg caccggaggg cctcagcgtg ctgggcaacg tggaccgcgc ggtcctgcgc
     4861 tccaaggggg gaaggggaac gggcgaccgc cagctggaga tggcggcggc cctcctgggg
     4921 cagagggaat gggaatggct gtcgtccctt ccggaggagc tgcgcctgga cgtgcgcggg
     4981 cgcaccctgc tgatcgtcca tgcctcgccg gggcaggagg aggagaggtt gctccctagc
     5041 acccccgaag cgcggctcag ggaggtcgtg gaaggagcgg gggcggacat catagtgacc
     5101 ggccatacgc atgtgcccat ggtccgggcc tgcggggggg cgctgttcgt caaccctggg
     5161 agcgtggggc ggccaaggga cggggcggag gcctcctatg ccctgctcga gcttccggcg
     5221 atgagggcgg agctgcaccg ggtggcctac gacgcggagg gcgtggccgg ggagctgcgt
     5281 tccatgggct tcaaggacct ccccgaggcc atcgtccatg gcatggaccc catgggcaca
     5341 ttgggctcct gggcggaagg gcttcacggg gacagggcgc acgcggagca ggtgcgcgcc
     5401 ctggccctgc tcctgttcga tcagacctgc gagctgcatt gcttgtcccc cagagaccgg
     5461 cagctgctgg agatggccgc cctggtccat gatgtggggc tgagggatgg gacggagggg
     5521 caccagcgga gggcgttgga cattgtcctg aaggctgata ttcccgtcaa tgcggacgac
     5581 cggctgatgc tcgcctgcgt ggccagatat catggccgtc gtccgccccg cgcctcggac
     5641 agggggttca gggacctcag ggagagggac cgcgaacggg tacggaagct ctccgccctc
     5701 cttgccctgg cggatggcct ggaccggggg catgcggcgc tggtgaactg gatcgaggtc
     5761 agcgttggga aggaggaggt cctgctccgg atcaagggga aagggaagtt gggcgcggag
     5821 agggaatggg ggctgcgcaa ggccgtccag ttcgagaggg cctttggtcg gaaggtgcgc
     5881 atagatggct gatatgaaga ggggcaaggt ggtggccttc atggacgtgg ggaccaattc
     5941 cgtgcggctg ctggtcgtcc gtatcggccc cgaccgctcc tacgccgtcc tgagcgagca
     6001 gaaggaaatg gtgaggcttg gagagcgcgt cttccgggac gggttgctgc gggaggaggc
     6061 catggagagg acggttagcg tgctcaagaa cttctgcgac ctctcccgca cctacggggc
     6121 gacggagctt gtggccaagg ccacctcggc cacccgggag gcttccaatc gccaggagct
     6181 aatagaccgg gtcagggagg ccacgggcct ggaactccag atcatctcgg ggctggagga
     6241 ggcgaggctg atctatttgg gagtatcgag cgggcaccat atcggccata ggaccgccct
     6301 cttcgtggac atcggcgggg gaagcaccga gctggccctc ggggaccagg acgaccattc
     6361 ctacctggag agcatgaagg tgggggcgat ccgcttgacg accatgttcg tcaggaggcc
     6421 ggagggcccg gtgcccgagg aggtcctcgg gaagatgcgc aaatacgtca agagctccct
     6481 ggtgcgggca aggaaggagg tcctgcagag gaaggtctcc ctggccttcg ggagctccgg
     6541 caccatcgtg aacctggggg aggtcctgct caggcgggga agccaccgtc ccggtgtgat
     6601 ccggcgctcc cagctgaagc ggctcatccc ttacctggcc gggatggacc tcgccaccag
     6661 gaggaaggtg ccgggcatca atccggagag ggcggacatc atcgtggccg gagctgtggt
     6721 cctggatacg gtgatggacg aactgaggct gagcgagatc gccgtgagcc cgcggggcat
     6781 gcgggacggg atgctgcagg attacctgag cgggatggag gggcctcctg gccttcgcga
     6841 gctctccgtc agggagagga gcgtcatcca gctcggtcgg gcgtgcttga tcgacgagga
     6901 gcatgcccgc agggtgacca tcatcgccct ggacctgttc gacagctcag agaggaaggg
     6961 attgcatgtc atgggcagga aggagcggga gctcctgcgc catgcggcct tcctgcatga
     7021 tgtcggggac ttcatctcct tcaacgacca tcatctccat tcccattaca tgatctccca
     7081 cgccgagctc ctcggcttct cccgccggga ggtgctgctc atggccaacg tggccatgta
     7141 ccatcggaag cgcatgccga aggtggggat ggcggaactg agggagctgg acgggaagga
     7201 ccaggaggcg gtggtccgcc tttccaccct gctccgcatg gcggagagcc tggataggtc
     7261 gcattccaac cttgtacagg gtgtccgctt cagcaaggtc accaagcgct cggcgaggct
     7321 gatcgtgacc gccagcgggg ac
//
LOCUS       DAOSYN010000053         7322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_8629_length_7322_cov_4.169121, whole genome shotgun sequence.
ACCESSION   DAOSYN010000053 DAOSYN010000000
VERSION     DAOSYN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8629_length_7322_cov_4.169121"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..837)
                     /locus_tag="PLJ11_03435"
     CDS             complement(391..837)
                     /locus_tag="PLJ11_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03752.1"
                     /translation="MKTNDRPFEGILGNTVELRILERLIASPDAEFNVAELSSMTGVH
                     RDSASKVINKFVGWNVLVRTTKGKMDFYRLNSDEPLVVSINAFNDSLIMQMFPEVRAA
                     MESVEDRSAISPPQEGSHTQHRTAIETASKRIAEKYARTLKRPAEP"
     gene            complement(917..1198)
                     /locus_tag="PLJ11_03440"
     CDS             complement(917..1198)
                     /locus_tag="PLJ11_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03753.1"
                     /translation="MTCGLSKDELDILTYLYKGRCLSPDHSISLKAIERDLGHHITDL
                     ENKLDVLVAKRYLGCKKKRSKNYYAGPSPTIKAISAHGVQIWWGGRGKI"
     gene            complement(1546..3120)
                     /locus_tag="PLJ11_03445"
     CDS             complement(1546..3120)
                     /locus_tag="PLJ11_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03754.1"
                     /translation="MRSLMAMVIVGFLAVMMISTSVPGVVSGDEFSFTENWPVFRPGD
                     YSTFVEPSMAVDLNGSIHLAYTYDPTPGDDYQDTGLHRYSVLLRYATLTNRTWSFSDL
                     GEEEWIQSVTVAVGNHGQPHIVCLQGANLTTWELVHWYRTNDTWTSETVRTFGHMGVT
                     GINTLVDSNDSVHLAFSAALESDFTFKTHVHMTNAGGNWTSDELATVRSSRAVWTNPP
                     QLAMDSQGRLHMAYLNVTDYVIEDWYSGNHSIEFVTLDDGEWGDRQVLNVGTLNRFSF
                     GVDTDGFESVMYQQSDQLWSSGGKVRLAIRYDFGWSYKPYDEDGTPFCKLIMDGDEER
                     MLYQRGSCLNRLEYYANNWIEEPLWIPDGMSLAIKEFAAGPWDHLVLLYFDSLTQSIA
                     VAQPGAQMELVPPTLNLKQDELEVNIYWEPMSTVFSGSCVIYRSGGASFVQQNGTNSF
                     VDDTLNEDSWRHTYQYRMSVLTPDGEEYVGPLTEVWIEPEFGQGALFDVLIAVTVVIA
                     VIVVFILYMNRGKLIR"
     gene            complement(3325..3894)
                     /locus_tag="PLJ11_03450"
     CDS             complement(3325..3894)
                     /locus_tag="PLJ11_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03755.1"
                     /translation="MHEDDHFFVGNNSDDSDRSLRLPAATYPASRDPGDMNDDGTVIL
                     LFDAMNEELLLYMPGSLSLYPEITDLDSTSTEVHLEWEPSGSSVTEVRIYHAALWGET
                     VPITVDSGMPFVFPADVTGMTDATIGSIQVWTLLNYRISVITAEGEYLGEMVTIHTEE
                     GWDEGLDMRDLWLMFAATAISSIAFTAVL"
     gene            complement(3943..4851)
                     /locus_tag="PLJ11_03455"
     CDS             complement(3943..4851)
                     /locus_tag="PLJ11_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03756.1"
                     /translation="MIISSLSFGPAQADDELQLENWPVFRAGPAGSTLEPSAVMDEEG
                     VVHTVYLYRNPAYSDWYLRPSFLRYANLSQGTWEHADLQAGFFEFADIALDAEGNVHI
                     LCATTWNYKIDVMYWNNTNGTWACQNVTQMTMGGARNADMAVDVEGKVHAVMGLRSLQ
                     GEENLTYYYVTNAREGWELMPFHYIDMYDGQTSELICMPDIEIDSTGKVHIAHVKVDN
                     AISGDWGSGDWSLQLLTRDGDSWKDEKNLSLPDRCNSLSLQIDADDNEHVLYHCVTSS
                     TEEDGPVRLAAWNDSGVSWGGIAEGT"
     gene            complement(5011..5412)
                     /locus_tag="PLJ11_03460"
     CDS             complement(5011..5412)
                     /locus_tag="PLJ11_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOO03757.1"
                     /translation="MTIARFWRENSPRYNLKASKCGNCGKISFPPRSVCPECHRRSIG
                     KMQEVQLSGKGEVYSFSVVHDAPAQFLNQRPYVVALIKLDEGVMLTAQIIDVEPGDVR
                     IGMRVWASFRKLGEEGPGGVIHYGYKFHPEL"
     gene            complement(5412..6578)
                     /locus_tag="PLJ11_03465"
     CDS             complement(5412..6578)
                     /locus_tag="PLJ11_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOO03758.1"
                     /translation="MRDVAIIGIGQTRFGELWDLSFREIGIQAGLAAIYDANVASEDI
                     EALYVGNMSAGRFIEQEHVAALIADYSGIARDHIPATRVEAAGASGALAVRQGYFDVA
                     SGLHDIVVVGGAEKMTDVPDAQGAAIQDSAADQQWECTFGATFPALHAMIARRYMHEH
                     GATREEIASVAVKNHKHGALNPKAQFQKEIKLETVLGSAPVAEPLRMFDCAPLSDGGA
                     AVVMCAMEKARRYTDSPVRMIASSQSSDTLALHHRRTITSFEATKVTMRKLVKRSGVD
                     ISKINLAEVHDNYTISELLALEDIGFFPPGEAGKATMDGQTEIGGRVAVNPSGGLKAR
                     GDPIGATGVAQIVELVTQLRGEAGKRQVADARYGLAQSVGGTGSTVVMHLLGVA"
     gene            complement(6575..>7322)
                     /locus_tag="PLJ11_03470"
     CDS             complement(6575..>7322)
                     /locus_tag="PLJ11_03470"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOO03759.1"
                     /translation="TVADYEFACKAGTAAIQTCMGLVGAGMIKYGVAIGADTSQGAPG
                     DALEYSASAGGAAFLIGPEKIIAELNLTYSFTTDTPDFWRREGQPYPSHGGRFTGEPA
                     YFKHVTACALGTLKKAGTKPSDYRYAVFHQPNGKFPSRVAKQLGFTEEQIRAGLLTPY
                     IGNTYSGAVPLGLSAVLDEAAPGDRIFAVAYGSGAGCDGFDITVTEEMSKYKRGNAPS
                     VRFMMESTKAIDYATYARYRGKIRMKEGGE"
BASE COUNT         1372 a         2223 c         2147 g         1580 t
ORIGIN      
        1 gagtgggaac cacagcgatg aaaggctttg attggccaca ccggctcttc gccagctgat
       61 acacgtcctt gaaagatgag gaatgggcaa tgaccctttc atcacatatg gcgacccatt
      121 gtcccgcgta tggggtgaca tccatgctca tgaagtgatc gtagttggtg gtcacgttct
      181 tcccgagatg ggattcgcta tgtcaggtaa taaacatcat cgctaattgt aatgggtcaa
      241 ttggcataga ttcggcgatc ttatcccagt tatccaatga tcacctggtc atcgagagat
      301 cattgttcat tgatgatagt gaccgaggag tgtgtcgacg gcaagaactt cgacctggac
      361 gtgaagtgat caggtccgat agatgatcgc tcacggctca gcaggtcgct tgagcgttct
      421 agcatacttc tccgcgatcc gtttcgatgc ggtctcgatc gctgttcggt gttgggtatg
      481 agacccctct tgcggcggag atatcgcgga tctgtcctca acgctttcca tagcggccct
      541 gacctcggga aacatctgca ttatcagtga atcgttgaag gcgttgatcg agacgaccag
      601 cggttcatcc gagttcaacc tgtagaagtc catcttgccc ttggtggtgc gcacgagaac
      661 gttccacccg acgaacttgt tgatcacctt ggaagcgctg tcccggtgaa ccccggtcat
      721 gctgctgagt tccgctacgt tgaactccgc atcgggagag gcgatgagcc tttccagtat
      781 cctcagctcc acggtgttgc ctagtatccc ttcgaacggc ctgtcattcg tcttcatgca
      841 tctcccctcg acgaccgtat acagatgcct atgacataaa tatgtcggat ataatcagca
      901 tttatgtcgt ataaaatcat atcttgcccc tccctcccca ccagatctgc acgccgtggg
      961 ccgagatggc cttgatggtc ggcgagggac cggcataata gttcttcgac ctcttcttct
     1021 tgcaccccag atagcgtttg gccacgagaa cgtccagctt gttctccaga tctgttatat
     1081 gatggcctag gtcgcgttcg atggccttca gactgatgga gtgatctggg ctaaggcagc
     1141 ggcccttgta aagataggtc agaatgtcaa gctcgtcttt gctgagtcca caggtcatca
     1201 aaggcacctg ctttcctgat aatgatggtg cattgtcccg ggagtataga tcacgatagt
     1261 gtgacagaag agctgatggt gtaatatcta tgattacaga tatattaact caaaaagatg
     1321 aaaaatgaaa acggtcacga gagggatcac ccttcgtaaa aaaaaatgaa taagctgtac
     1381 ttgggtcact actgcggaca gaacatcatg atgtcccatg cgacagcaag ttcgacctgg
     1441 acgtgaagtg aggttggccg atttattgaa aatcgtcctg aatgcggacg gaaatggttt
     1501 attaccggcc tattccagga cgatttgagg aataatctga gagattcacc tgatgagctt
     1561 gccccggttc atgtacagga tgaacaccac gatgaccgct atcaccaccg tcacggcgat
     1621 gagaacgtcg aacaatgccc cctggccgaa ctccggttcg atccaaactt ccgtcaacgg
     1681 cccgacgtac tcctctccgt ccggggtcaa gacgctcatc cggtactgat aggtgtgacg
     1741 ccagctgtcc tcgttcaacg tgtcgtccac gaagctgttc gttccgttct gctgaacgaa
     1801 cgatgcgccg ccgctgcggt agatgacaca gcttccagag aacacggtgc tcatcggttc
     1861 ccaatatatg ttcacctcca gttcgtcctg cttcagattg agggtgggcg ggacgagttc
     1921 catttgagcg cccggctgcg ccacggctat gctctgcgtg aggctgtcga agtaaagaag
     1981 aacgagatgg tcccaggggc cggcggcgaa ctctttgata gcgagcgaca ttccgtccgg
     2041 tatccacagg ggctcttcga tccagttgtt cgcgtaatat tccagacggt tgaggcagct
     2101 tcccctctgg tacagcattc tttcttcatc tccgtccatt atgagcttgc agaacggggt
     2161 gccgtcctcg tcgtagggtt tgtaagacca gccgaagtcg taccttatcg ccaatcgcac
     2221 cttgccgccg gagctccaca actggtcgct ctgctggtac atgacgctct cgaaaccgtc
     2281 cgtgtccacc ccgaaggaga agcggttgag ggtgccgacg ttgagcacct ggcggtcgcc
     2341 ccattctccg tcatccagcg tcacgaactc gatggagtgg ttcccggaat accagtcctc
     2401 gatgacgtaa tcggtcacat tgagataggc catgtgcaat ctaccttggc tgtccatggc
     2461 cagctgcggc gggttcgtcc acacggcgcg ggaggagcgg acggtcgcca gctcgtccga
     2521 cgtccagttg cccccggcgt tggtcatgtg cacgtgggtt ttaaaagtga agtcgctttc
     2581 caaggcggcc gagaaggcca ggtgaacgct gtcgttggag tccaccagcg tgttgatgcc
     2641 ggtaacgccc atgtggccga aggtgcgcac cgtttcggag gtccatgtat cgttcgtcct
     2701 ataccagtgc accagctccc aagtggtgag attggctccc tgcaggcaca cgatgtgcgg
     2761 ctgaccgtgg ttacctacgg ccaccgtcac gctctggatc cattcttcct cgcccaggtc
     2821 cgagaagctc caggtcctgt tcgtcagcgt tgcgtaacga agcaggacgg agtacctgtg
     2881 caatcctgta tcctggtaat cgtctccagg tgtcgggtca tacgtataag ccaggtggat
     2941 cgagccgttg aggtccacgg ccatgctcgg ctcgacgaac gtgctgtaat caccgggacg
     3001 gaacaccggc cagttctccg tgaacgaaaa ttcatcgccg ctaaccaccc ccggtacgct
     3061 ggtcgatatc atcatcacgg ccaggaatcc cactatgacc atcgccatca acgatctcat
     3121 ttactatccc tcgatcgggt tcgcgaccat gagcggcgct gcgaccgctg atccggatat
     3181 cgccttccgt tccactcccg cttacccggt gatgcaggat ttggtctgag cataaattca
     3241 tcggtgcctc ttcctgaagc gataaacggc gacatcgggt tcgatcacga tagctgacca
     3301 tcatctcttc ttccggatca acagctacaa caccgcggtg aaggcgatcg aggatatcgc
     3361 ggtcgcggcg aacatcagcc agaggtcgcg catgtccaga ccttcgtccc atccttcctc
     3421 ggtgtggatg gttaccatct cccccaggta ctccccttcg gcggtgatga cgctgatgcg
     3481 gtaattcagc agggtccaga cctgtatgct gcctatggtg gcgtcggtca tgccggtgac
     3541 atcggccgga aatacgaacg gcatgccgga gtcgacggtt atggggacgg tttcgcccca
     3601 gagggccgca tggtatatcc tgacctcggt cacgctcgat ccgctcggct cccattcgag
     3661 gtgaacctcc gtactggtgc tgtcgaggtc ggtgatctcc gggtacagcg acaagctccc
     3721 gggcatgtaa agtagcagct cctcgttcat ggcgtcgaac agaaggatga ccgtgccgtc
     3781 gtcgttcata tcgccggggt cgcgggaggc cgggtaggtt gcggccggca agcgcaggga
     3841 gcggtcgcta tcgtcgctat tgttgccgac gaagaagtga tcgtcctcgt gcatgaagat
     3901 gaaacgctcg cttccatccg cgttcagatg aagctcgcac tgtcaggttc cctcggcgat
     3961 gccgccccag gaaacaccgg aatcgttcca ggcggcaagc cttaccggtc cgtcctcttc
     4021 cgttgaggat gtgacgcaat ggtacagaac atgctcattg tcatcggcat cgatctgcag
     4081 cgacaggctg ttgcaccggt ctggcaagct caaattcttc tcatccttcc agctgtctcc
     4141 gtcgcgggtg agcagctgca acgaccaatc tccggagccc cagtcgccgg agatggcatt
     4201 gtcgaccttg acgtgggcta tatgcacttt tcccgtgctg tcgatctcga tgtcgggcat
     4261 acatatcagt tcgctggtct ggccgtcgta catgtctatg taatggaagg gcatgagctc
     4321 ccacccctcc cgagcgttgg tgacataata ataggtcaag ttctcctcgc cttggagaga
     4381 gcggagcccc atgaccgcgt gcacctttcc ctcgacatcc accgccatat ccgcgttcct
     4441 cgcgccaccc atggtcatct gtgtaacgtt ctggcaggcc caggtcccgt tggtgttgtt
     4501 ccagtacatg acgtcaattt tgtagttcca tgtggtggcg catagaatgt gcacgttccc
     4561 ttcggcgtcc agggcgatgt cggcgaattc gaagaaaccc gcctggaggt cggcatgctc
     4621 ccaggttcct tgcgagagat tggcgtagcg caggaagctc ggtcggaggt accagtcaga
     4681 gtatgctgga ttgcggtaaa ggtataccgt atgaacgacg ccttcttcgt ccatgaccgc
     4741 cgacggttcg agcgtggagc cagctggtcc ggcgcggaac accggccagt tctccagctg
     4801 aagttcgtca tcggcctgcg ccggtccgaa cgataaggac gagatgatca tcaaaaagga
     4861 tagcgcgacc gctgccatga tggcggtcct ggagaacgca gtgatgcgtg acgctccgac
     4921 ttcccccggg gaactactag cgtgggggat aaagaagcat cggtcgattt tgtagaaaaa
     4981 tgtgttcgag aaggggtttt tcgttcgggt tcaaagttcc ggatggaact tgtagccgta
     5041 gtggatgacg ccccccggcc cctcctcccc gagcttgcgg aaggaggccc agacccgcat
     5101 gccgatccgc acgtccccag gctcgacgtc gatgatctgc gccgtgagca tgaccccctc
     5161 gtccagcttg atgagcgcga cgacgtaggg cctctggttc aggaactgcg ccggggcgtc
     5221 gtgcaccacg ctgaacgagt acacctcccc cttcccggac aattggacct cctgcatctt
     5281 gccgatagac cgccgatggc actccgggca cacggagcgc ggcgggaacg atatcttgcc
     5341 gcagttgccg cacttggacg ccttgaggtt gtaccggggc gagttctccc gccagaagcg
     5401 cgcgatcgtc atcaggccac ccccagcagg tgcatgacga ccgtggagcc ggtgccgccg
     5461 acgctctgcg ccaggccgta cctggcgtcg gccacctggc gcttccccgc ctcgccccgc
     5521 agctgcgtca ccagctccac gatctgcgcc acgccggtgg cgccgatcgg gtcgcccctc
     5581 gccttcagcc ccccggaggg gttgacggcg accctgccgc cgatctccgt ctgcccgtcc
     5641 atcgtcgcct tccccgcttc gcccggcggg aagaagccga tgtcctccag ggccaggagc
     5701 tcggagatgg tgtagttgtc gtgcacctcg gccaggttga tcttggagat gtcgaccccg
     5761 gagcgcttga cgagcttgcg catcgtgacc ttggtggcct cgaacgaggt gatggtgcgc
     5821 cggtggtgca gcgccagcgt gtcggacgac tgcgaggacg ctatcattcg caccggcgaa
     5881 tccgtgtacc tccgggcctt ctccatggcg cacatgacca cggccgcccc gccgtcggag
     5941 aggggagcgc agtcgaacat gcgcagcggc tcggcgaccg gggcggagcc gagcacggtc
     6001 tccagcttga tctccttctg gaactgcgcc ttggggttca gcgcgccgtg cttgtggttc
     6061 ttgaccgcca cggaggcgat ctcctccctc gtcgcgccgt gctcgtgcat gtaccggcgg
     6121 gcgatcatgg cgtggagcgc cgggaaggtc gcgccgaagg tgcactccca ctgctggtcc
     6181 gccgcggagt cctggatggc ggccccttgc gcgtccggca cgtccgtcat cttctccgcc
     6241 ccgccgacga cgacgatgtc gtgcagcccg gaggcgacgt cgaagtaccc ctggcggacc
     6301 gccagggccc cggaggcgcc ggccgcctcg accctcgtgg ccgggatgtg gtccctcgct
     6361 atgcccgaat agtcggcgat gagcgccgcg acatgctcct gctctatgaa gcgcccggcg
     6421 gacatgttgc cgacgtacag cgcctcgatg tcctccgagg ccacgttggc gtcgtagatg
     6481 gccgccagcc cggcctggat gccgatctcg cggaaggaga ggtcccagag ctccccgaac
     6541 ctcgtctgcc cgatgccgat gatcgccacg tccctcattc ccctccctcc ttcatcctga
     6601 tcttcccgcg atacctggcg tacgtcgcgt agtcgatggc cttcgtgctc tccatcatga
     6661 acctgacgct cggggcgttg ccccgcttgt acttggacat ctcctccgtc accgtgatgt
     6721 cgaacccgtc gcaccccgcg ccggagccgt aggccacggc gaagatgcgg tcccccggcg
     6781 ccgcttcgtc cagaacggcc gagaggccca ggggcaccgc cccggagtag gtgttgccta
     6841 tgtacggcgt gagcagcccg gccctgatct gctcctccgt gaagccgagc tgcttggcga
     6901 cgcgggacgg aaacttgccg ttgggctggt ggaagacggc gtacctgtag tcggacggtt
     6961 tcgttcctgc cttcttcaag gtccccaggg cgcaggcggt gacgtgcttg aagtacgccg
     7021 gctccccggt gaagcgcccc ccgtgcgagg ggtacggctg cccctccctc cgccagaagt
     7081 ccggggtgtc cgtggtgaac gagtacgtga ggttcagctc cgctatgatc ttctcagggc
     7141 cgatcaggaa cgccgccccg ccggcggacg ccgagtactc caatgcgtcg cccggggccc
     7201 cttgggacgt gtccgcgccg atcgccacgc cgtatttgat catcccggcg ccgacgaggc
     7261 ccatgcacgt ctggatggcg gcggtgccgg ccttgcaggc gaactcgtag tccgcgacgg
     7321 tg
//
LOCUS       DAOSYN010000054         7226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_8775_length_7226_cov_4.006136, whole genome shotgun sequence.
ACCESSION   DAOSYN010000054 DAOSYN010000000
VERSION     DAOSYN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8775_length_7226_cov_4.006136"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /gene="nrdD"
                     /locus_tag="PLJ11_03475"
     CDS             <1..360
                     /gene="nrdD"
                     /locus_tag="PLJ11_03475"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOO03760.1"
                     /translation="GAQISAIERVKTEGLFHPLVEAGALTHVWLGEHKPPAENLAAFV
                     IKTFQNTQNAQIAFSPEFTSCLSCGKTARGLSDTCVYCHSPDVEGITRVTGFFSKTSG
                     WNKGKLGELKERTKNII"
     gene            462..740
                     /locus_tag="PLJ11_03480"
     CDS             462..740
                     /locus_tag="PLJ11_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03761.1"
                     /translation="MVRIQVSEHRFVEIRRRSLCDNCSVRNCTSFDGSRVTECAGFKP
                     LFLVLLKCRECGRVYDPYQSLRSLDYALCPECNHINKGEAMISFVCRE"
     gene            complement(741..2351)
                     /locus_tag="PLJ11_03485"
     CDS             complement(741..2351)
                     /locus_tag="PLJ11_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO03762.1"
                     /translation="MYESLLLVLLAICAFVLIVRTADRKGWIKRYGMKAYGPFLMWST
                     RRGKGALDLLARPKRLWRAYGAVAKWVCAIVMVLMMALLLWEATLVDQIPAESAPGLE
                     LMLGIPGVNPIIPLWYGILGLIVAIVIHEGAHGILTRVGNMEVRSMGLIFLLVPLGAF
                     VEPDEEALVKADKRRRMSVYAVGPATNIIVGLVCALLLSSMLIGSVEPVRESPIVISL
                     ADDGPADRAGLRFGAQIVQLNGTEILTYEEFQSFPGPDPGTNITISYYYNGELRTTEV
                     TAGVMLTSVSSDYPAAKAGLRAGMLLHSVNGTVIRNDADLKEVLRNTVGGQTIPVTVL
                     VYDKSVGTYVPEPTVRNVTLMSRLAYYQEVSPGSIGEDFEDYGFMGINSAYMGAGVNS
                     PEIILDRIAPPIGEVDDFGSFVSASLGYIALPFYGLAPLTGSITELFEPQGAWGALPD
                     SAFWVLVNSLYWIFWINIMVGMTNVLPAVPLDGGYLFRDGLDALVQKVRKGASEQERM
                     RSVATITNALSIFVLFLILWQLIGPRLG"
     gene            complement(2386..2760)
                     /locus_tag="PLJ11_03490"
     CDS             complement(2386..2760)
                     /locus_tag="PLJ11_03490"
                     /inference="COORDINATES: protein motif:HMM:NF039321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03763.1"
                     /translation="MTMDDVVKALGNTMGKRGMSREDQEKLAEWILSFFGYTDEVIDN
                     KLTSEDRDVFYWLEEEGFLTTTQEEVHLKKGKLWRIHYWILKKDQILRMAHQAEEGAG
                     GPEDPSTVYNNMNDEMWKARER"
     gene            complement(2836..3180)
                     /locus_tag="PLJ11_03495"
     CDS             complement(2836..3180)
                     /locus_tag="PLJ11_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03764.1"
                     /translation="MASKRRLLVDTSKQQGELQGGGAEEEVEDRRTDRDIMIEGTWRE
                     WFLLVFLKYCYVVALLFIACIVPLEIVRVIDGSIGIALAFVAILIIVPLGAVAYLRLW
                     GNEGRLGKGGPV"
     gene            complement(3187..4284)
                     /locus_tag="PLJ11_03500"
     CDS             complement(3187..4284)
                     /locus_tag="PLJ11_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO03765.1"
                     /translation="MSDDVLFEIRHLQKYFPIRGGILGRRLGNVKAVDNISLSIYKGG
                     TLGVVGESGCGKTTTGRCILMLTPPTDGNIYYQMPCDARKRMLELEAEEKTLFDKNPN
                     AKKKKDGIIPELEEIRDSYALNRKSVDELRHLRKNMQIVFQDPYSSLNPRMLVKDIIA
                     EPMKIHGISGNKSNITGRVKELMERVGLSPEHIYRYPHEFSGGQRQRIGVAKALALNP
                     SFIVLDEPTSALDVSVQSQILNLLNDLQRELDLTYMFISHDLSTIKYMCDSICIMYLG
                     KIVEMGTKDAVFGKPLHPYTEALLNSIPVPDPKLKRKRVPLSGDIPSPANPPHGCRFH
                     TRCRYREAICEEVEPQLEDKGNGHMVACHFR"
     gene            complement(4277..5686)
                     /locus_tag="PLJ11_03505"
     CDS             complement(4277..5686)
                     /locus_tag="PLJ11_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO03766.1"
                     /translation="MMDNGKNFLTISDLIVNFYTKAGVVKVIDGVDLKINHGETLGLV
                     GESGCGKSVTAYSIMRLIPSPPGKIEHGMILMDLPEELYAKLSTIEQEKRKGASPEEI
                     KKKREELAPELRKYDILMRSQYAMRKIRGKQISMIFQEPMSALNPVFTIGFQITEILY
                     LHEMPDLIKGTLRRLEESRNDATGLPDGQQTEGEEMKCSSCGSTLNGSSHRCPYCGVD
                     CNRSKKNGLTIWNIKRKEKALKRMLKDQDNIVLTIMNKIPFINSYKKDLEGEAMGRAI
                     RMLRLVRIPDPGSIVKNYPHELSGGMQQRVMIAMALACKPKLLIADEPTTALDVTIQA
                     QILKLMRELQEEMGTTILLITHNLGVVAEVCERIGVMYAGNIVEIGKTDDIFAEPLHP
                     YTQGLMHSIPRITGASDRLDVITGSVPNLLNPPSGCRFHPRCPFSMPICSEIKPILGN
                     IREDHQVACHLYGMVKKNE"
     gene            complement(5689..6669)
                     /locus_tag="PLJ11_03510"
     CDS             complement(5689..6669)
                     /locus_tag="PLJ11_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO03767.1"
                     /translation="MANKKKVHKKKAVKTEQAMPKKRSRNPEVEELIAELRPRIKEYS
                     DSLSMVVHNPTVMLGILIISIIIIIALFAPFIAPPADNSDPYLIPMDFIVPPRAPGVD
                     GFILGSGDLGLDVFYGIVWGARTTIYTSLLVVGIATIIGLLLGSVAGFYGGRIDNILM
                     RITDVFLSLPALILAMAVSSVLSKDINSIIFSLIIVWWPSYARLVRGQVLAIKENTYI
                     EAARAIGAKNSRILFRHVVPNSLTPLIVAVTMDIGSVALTAAGLSYIGFGVETGYAEW
                     GRMVSDGQKWFVAGYWWTVAFPGLAILLFTMGFSLVGDGLRDILDPRMKR"
     gene            complement(6674..>7226)
                     /locus_tag="PLJ11_03515"
     CDS             complement(6674..>7226)
                     /locus_tag="PLJ11_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO03768.1"
                     /translation="FYQLNLLPAINIYNPVEYSAADIPHITNFLIIDSLLVGNFGLFI
                     DHIAHMVMPAVTLSFGIIAILIRIQRNSMLEVLNLDYIKTARAKGLPEKTVINKHARR
                     NALIPTTTVIGLSFGASLGGAVLTENIFALPGLGKWSANSITHFDSTSILGFVLFIAL
                     VYSLVNLVVDIMYAYLDPRVKLG"
BASE COUNT         1625 a         2036 c         1862 g         1703 t
ORIGIN      
        1 ggggcgcaga taagcgccat cgagcgggtg aagacggagg gactgttcca ccccttggtg
       61 gaggcgggcg ccctcaccca cgtctggctt ggagagcaca agcccccggc ggagaacctg
      121 gcggccttcg tcatcaagac cttccagaac acgcagaacg cgcagatagc cttcagcccg
      181 gagttcacct cctgcctgag ctgcgggaag accgcgcgcg gcctttccga tacctgcgtc
      241 tactgccact ccccggacgt ggaggggatc acccgggtca ccgggttttt ctccaagacc
      301 tccggatgga acaaggggaa gctgggggag ctcaaggagc ggaccaagaa catcatttga
      361 gcggtcggcg aagccccgcc ccctcccttt ctttttttat ttacggggca gcgctccaaa
      421 cctttatgta accataacat gaaagtaagg aagggacgtg tatggtgcgc atacaggtgt
      481 cggagcatcg gttcgtcgag atcaggcgga ggagcctgtg cgacaactgc tcggtgcgca
      541 attgcacgtc cttcgatggg agccgggtga ccgagtgcgc cgggttcaaa ccgctgttcc
      601 tggtcctttt gaagtgccgg gaatgcgggc gggtgtacga cccctatcag agcctccgtt
      661 ccctggacta cgccctctgc ccggagtgca atcacatcaa taagggcgag gctatgatct
      721 ccttcgtctg ccgggaatga tcatcccagc cgcgggccga tgagctgcca taggatcagg
      781 aagaggacga atatgctgag ggcgttggtg atcgttgcca cggaccgcat acgctcctgt
      841 tcgctcgcgc cctttctgac cttttgcacc aacgcatcga gcccgtcgcg gaagaggtac
      901 ccgccgtcca ggggaaccgc cggcagcacg ttggtcatgc ccaccattat gtttatccag
      961 aatatccaat agaggctgtt caccaggacc cagaacgcgc tgtccggaag ggcgccccag
     1021 gccccctgag gctcgaagag ctcggtaatg ctgcccgtca gtggggccag gccgtagaac
     1081 gggagcgcga tgtagcccag ggaggcggag acgaacgaac cgaagtcgtc cacttccccg
     1141 atcgggggcg cgatgcggtc cagtatgatc tcgggggagt tgacccccgc ccccatgtag
     1201 gcggagttga tccccatgaa cccatagtcc tcgaagtcct cgccgatgga gcccgggctg
     1261 acctcctggt aataggccag acggctcatc agggtgacgt tgcgcaccgt cggctcgggc
     1321 acgtacgtcc ccaccgactt gtcatacaca aggacggtga ccgggatggt ctgcccgccg
     1381 accgtgttcc tcaggacctc cttcaggtcg gcatcgttcc tgatgacggt gccgttcacc
     1441 gaatggagga gcatcccggc cctgagcccc gccttggccg caggataatc gctcgacacc
     1501 gaggtgagca tcacccctgc cgtcacctcg gtcgtccgga gttcgccatt gtagtaatag
     1561 gaaatggtga tgttcgtccc tgggtccggc cccgggaagg attggaattc ctcgtaggtg
     1621 aggatttccg tcccgtttaa ctgaacgatc tgggccccga agcgcagccc ggcccggtcg
     1681 gcagggccgt catcggccag gctgatgacg atggggctct ccctgaccgg ctctacggac
     1741 cctatgagca tcgacgagag gaggagcgcg cacaccagcc cgacgatgat gttggtggcc
     1801 ggccccacgg catagacgct catgcggcgg cgcttgtcgg ccttcacgag cgcctcctcg
     1861 tcgggctcca cgaacgcccc cagggggacg agcaggaaga tgaggcccat cgacctcacc
     1921 tccatgttcc ccaccctggt gaggatgccg tgcgccccct cgtggatcac gatcgccacg
     1981 atcaggccca gaatgccata ccatagcgga atgatcgggt tcaccccggg gatcccgagc
     2041 atcagctcca ggccgggggc gctctccgcc gggatctggt ccaccagggt ggcctcccag
     2101 agcagcaggg ccatcatcag gaccatgacg atggcgcaca cccatttcgc caccgccccg
     2161 taggcgcgcc aaagcctctt gggccgtgcg aggaggtcca gcgccccctt ccccctccgg
     2221 gtgctccaca tgaggaaggg gccgtaggcc ttcatcccat acctcttgat ccagcctttg
     2281 cggtcggccg tgcgcacgat gaggacgaac gcacagatgg ccagcaggac cagaagtagg
     2341 gattcgtaca tctaggggca ggaaaggggg ctgtgtataa aatacctagc gttccctggc
     2401 cttccacatc tcgtcgttca tgttgttgta cacggtggac gggtcctcgg gcccgccggc
     2461 gccctcctcg gcctggtgcg ccatcctcag gatctggtcc ttcttgagga tccagtagtg
     2521 gatgcgccag agcttcccct tcttcaggtg gacctcctcc tgggtggtgg tcaggaagcc
     2581 ctcttcctcc agccagtaga acacgtcgcg gtcctctgag gtgagcttgt tgtcgatgac
     2641 ctcgtcggtg tagccgaaga acgagaggat ccactccgcg agcttctcct ggtcctcgcg
     2701 ggacatcccc cgtttgccca tggtgttgcc cagggctttc accacgtcgt ccatcgtcac
     2761 gaccgtcaag cgtgcctccc ccggaggcga atcgaagtcg acgtataaat taactgagtt
     2821 tactttagag ccgggctaaa cggggccgcc cttacccaat cgcccctcgt tcccccagag
     2881 acggagatag gcgaccgcgc ccagggggac gatgatgaga atggcaacga atgccagggc
     2941 gatgccaatg ctgccgtcga tcacgcgcac gatctccagc ggcactatgc aggcgatgaa
     3001 taggagagcg acgacgtagc agtattttag gaagacgaga aggaaccact cccgccaggt
     3061 gccctcgatc atgatgtccc gatcggtcct gcggtcctcg acctcctcct cggcccctcc
     3121 tccctgcaat tccccctgct gtttggaggt atctacaagg agccggcgct tgctcgccat
     3181 ggtccctcag cggaagtggc aggcgaccat gtgcccgttg cccttgtcct cgagctgggg
     3241 ctcgacctcc tcgcagatgg cctcgcggta ccggcagcgc gtgtggaacc ggcagccatg
     3301 gggcggattg gcgggggatg gtatgtcacc acttaaaggc acccttttcc ttttgagctt
     3361 aggatcagga acgggaatag agttcaatag agcctctgta tagggatgca aaggcttgcc
     3421 aaatactgca tccttggttc ccatttcaac gatcttgcca aggtacatga tgcaaatgga
     3481 atcgcacatg tatttgatgg tcgacaggtc atgggagatg aacatatagg tgagatccaa
     3541 ttccctctgt aaatcattca ataggttgag tatttgcgat tgtacagata catcgagggc
     3601 cgaggtaggt tcatccaata caatgaagct tgggttgagg gctaaggcct tcgccactcc
     3661 aatcctctgc ctttgccctc cactgaactc atgtgggtaa cggtaaatgt gctctggaga
     3721 aagccccacc ctttccatta gttcctttac cctccccgtg atgttgctct tgttcccaga
     3781 tataccgtga atcttcatag gctcggcgat tatgtccttg acgagcatgc gcggattcaa
     3841 tgatgaatag ggatcttgaa aaacaatctg catattcttc cgaagatgcc gaagctcatc
     3901 tacacttttt ctgttcaggg catacgaatc tcggatctcc tcgagctccg gaattatacc
     3961 gtctttcttt ttttttgcat tcgggttttt atcaaagagg gttttctctt cagcctctag
     4021 ctccaacatt ctcttccgcg catcgcatgg catttgatag tagatatttc catcagtagg
     4081 aggtgtgagc attaagatgc atctacccgt ggtggtcttc ccgcagccgc tttcgcctac
     4141 cactccgagg gtaccgcctt tgtatatgct aagactaata ttatctactg ccttgacgtt
     4201 tcctaaacgt ctaccaagga tgccgcctct gatgggaaaa tatttttgca gatggcggat
     4261 ctcaaacaag acgtcatcac tcattcttct tcaccattcc atacagatgg caggctacct
     4321 gatggtcctc cctaatattc cctaggattg gtttaatttc actgcatatc ggcatagaaa
     4381 acgggcatct cggatgaaaa cgacaacctg aagggggatt caatagattg ggaacgctgc
     4441 ctgtaatcac gtctaaccgg tcagaagcgc cagttatccg tgggatcgag tgcattaacc
     4501 cctgcgtata ggggtgtaag ggctcagcga aaatatcgtc cgtttttcct atttccacta
     4561 tattacctgc atacatcaca ccgatgcgtt cgcatacttc cgctacaacc cccagattat
     4621 gagtgatcaa taggatggtc gtgcccattt cctcttggag ttccctcatg agcttcaaga
     4681 tctgggcttg aatagtgaca tccagggcag tggtcggttc atcggcgatc agtaatttag
     4741 gcttgcatgc caaggccatg gcgatcatta ccctctgttg catccctcca gaaagctcat
     4801 gtggatagtt cttcactatg ctgcctgggt ctggtatacg gaccagcctg agcatgcgaa
     4861 tggctctgcc catggcctcc ccttccagat cctttttata tgaatttata aaaggaatct
     4921 tattcattat agtcaagaca atgttgtcct ggtctttcag catccttttc aatgcttttt
     4981 ccttgcgctt gatgttccag atggtcaatc cattcttttt cgaacgatta caatcaactc
     5041 cacaatatgg acaacgatgg ctcgaaccgt ttagggttga tccacaacta gaacacttca
     5101 tttcctcacc ctcggtctgt tggccatcag gaagtccggt cgcatcattg cgcgattcct
     5161 ccaatctgcg caaggtgcct ttgatcaagt caggcatttc atgaagatat aatatctcgg
     5221 ttatctggaa gccgatcgtg aacactggat tgagagccga catcggttct tggaagatca
     5281 tcgatatttg ttttccgcga atcttccgca ttgcgtattg tgatctcata agaatgtcat
     5341 acttcctgag ttccggtgca agttcctccc tctttttttt gatctcttca gggctggcgc
     5401 cttttctctt ctcttgctcg atggtactaa gcttcgcata gagttcctca ggaagatcca
     5461 tcagaatcat tccgtgttca atttttccag gtggggatgg gattaagcgc ataatgctgt
     5521 aggccgtgac gctttttcca catccgctct ctcctaccag ccccaaggtc tcaccgtgat
     5581 tgatcttcag gtccacgcca tcgatgactt tgaccacacc agccttggtg tagaagttga
     5641 cgataaggtc gcttatagtt aggaagtttt taccattgtc catcatgatc acctcttcat
     5701 cctagggtcc agtatgtccc tcaacccgtc accgaccaag ctgaagccca tagtgaagag
     5761 caggattgca aggcctggaa aggcaaccgt ccaccaatat ccagccacaa accatttctg
     5821 tccgtcggat accatcctcc cccattccgc atagccagtt tccacaccaa aaccaatata
     5881 acttaagcct gcagcggtta aggcaactga acctatgtcc atggtgaccg ccacaatgag
     5941 cggggtcaat gaattaggga cgacatgacg gaacagtatg cggctgttct tcgccccaat
     6001 tgccctcgcc gcctctatat acgtgttctc tttgatcgca agaacctgcc cgcggaccaa
     6061 cctcgcatat gatggccacc acactatgat aagagagaat attatactgt tgatatcctt
     6121 actaagtacg ctagatacag ccatggccaa gatcaaagct ggcaacgaca ggaagacatc
     6181 tgttattcgc ataaggatat tgtctatcct ccctccatag aagcctgcca cagaacccag
     6241 cagtaaacct attatcgtgg caatcccgac tactaataga gaggtatata tcgtggtcct
     6301 tgcgccccaa acgatcccat agaaaacgtc caagcccagg tccccacttc ctaatatgaa
     6361 accatccaca ccaggcgctc ttggaggcac gatgaaatcc attggtatta gatacggatc
     6421 gctattatca gctggcggag caatgaaggg ggcgaagagc gcgattatta tgataatgga
     6481 aatgatcaga atccccagca tcacggttgg attgtgcacg accatactga gggaatcact
     6541 atactccttg atacgaggcc taagctcggc aattagctct tctacttcag ggttcctaga
     6601 ccttttcttc ggcatggcct gttcggtctt cacggccttt ttcttatgaa ccttcttttt
     6661 gttggccatg atatcaccct aattttaccc ttgggtctag gtatgcatac ataatatcta
     6721 ccactaaatt gaccaaagag tacaccaagg caataaacaa gacaaagccc aatatggacg
     6781 ttgaatcaaa atgcgtaata gaatttgccg accactttcc aagcccggga agggcaaaaa
     6841 tattctctgt gaggaccgca ccccctaaag aagcgccgaa agatagacca atgacggtgg
     6901 ttgttggaat caaggcattc ctacgagcgt gtttattgat aacagtcttt tcaggaagcc
     6961 ctttcgctct ggcggtctta atatagtcca gatttaagac ctccagcata ctgtttcttt
     7021 gaatgcggat taaaatggcg ataataccaa aagaaagagt cactgccggc atcaccatat
     7081 gagcgatatg gtctatgaat aggccaaagt tgccaaccaa tagcgagtcg atgatgagaa
     7141 aattagtgat atgggggata tccgcggctg aatattcaac agggttgtag atattgatag
     7201 ccggtaacaa attcaattga taaaag
//
LOCUS       DAOSYN010000055         7052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_9030_length_7052_cov_11.849793, whole genome shotgun sequence.
ACCESSION   DAOSYN010000055 DAOSYN010000000
VERSION     DAOSYN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9030_length_7052_cov_11.849793"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLJ11_03520"
     CDS             <1..1130
                     /locus_tag="PLJ11_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HOO03769.1"
                     /translation="RGGGKTRAGSEWVLQRVRDGYRSIALVGKTAADVRDTMIELGES
                     SIMKVARPHERPLYEPSKRRLVFPNGAVAVAYNGDEPDQLRGPQHDSAWIDELAKFRY
                     PEECWDNLMFGLRLGPNPQAFVTTTPRPIPIIRKLIADKDTIDVRFSTFDNAENLSPI
                     FLQRVRERYEGTRLGRQELYGEILDDNPGALWKRDVIENLRVREHPPLVRIIVAVDPA
                     VTHNEDSDETGIVTAGIAASGHCYVLDDSSLQGSANQWAVAAVRAYHKWEADRIVGEV
                     NNGGDLVEVNIRTVDPGVPYEAVTASRGKLIRAEPVSALYEQGKVHHVGTFPQLEDQM
                     VEWVPGERSPDRMDALVWAITKLIPAAQTADPSRFLRYAVR"
     gene            1211..2413
                     /locus_tag="PLJ11_03525"
     CDS             1211..2413
                     /locus_tag="PLJ11_03525"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOO03770.1"
                     /translation="MRGSSGPYSPSFFDRVWIRLNEIVNPPVRPRTIAGTRRQRTGAD
                     LFSKRERSYKELDALEVRYLQGGPVREAIDSYPLFALSNGWYLDGKDEMLVKDTEAQL
                     AALDIESSIWQGIIDSLVFGDAFQEIVTGRGQYSDTVLALQPRPAKMFDIDADEYGVK
                     TGYRQYLDQEGRKYIPLALDQILHISLFHIGGSVYGTSLVNSAKDDIDRDVQMIESLV
                     SAIERHGHPRYHAKVGKEGEDVGQPVLDSVADQLHDLKSNTELATCHDVEIVSLDTAG
                     VGNTQAYSDLTLGRMACALGVPLDVLGVTEGSNRSTATVRQKVFETKISTIQRRIENI
                     YNSNIIDRLTGQPGSVRFKFNDISPEDELREVEYVAKVLAADPISPIVGHEWARRRLR
                     IPEEDAGP"
     gene            2410..3135
                     /locus_tag="PLJ11_03530"
     CDS             2410..3135
                     /locus_tag="PLJ11_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HOO03771.1"
                     /translation="MTSPGRIDPRDPSRLRATEERRARIAERDIKEAVDAAVKRVIEG
                     NITDPKTIQAIVQEELGRYESVASDRYRSWTSDTVQRSVLRAEQLLRASGVRIDAAFG
                     PRPISPQLRDALTLNVQNQIESLTASTKAKVTSALIESMSAGDGAKAAAKRVSEATGM
                     ERSRASLIARTETMRAYNQVNEDQFRKHDIAAVEWLAAIDERTCDVCGAHHGQRYSID
                     KHPEIPAHPNCRCILVPVIEGAG"
     gene            3135..4193
                     /locus_tag="PLJ11_03535"
     CDS             3135..4193
                     /locus_tag="PLJ11_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03772.1"
                     /translation="MPYRTIADLPENIRNSLTEEEAKRFIAAFNAAYKDGKDERTCFK
                     IAMTAAKKREASAAHSAMRMLNRTLSFSNYIEVDGGMLVKDVPLLAVGEWTDSAVGTP
                     LYYPAKVLERDALNWYDNSFWARHGGKVPRSVVSDLIGEVRNIRYDGSYKENGMVEPG
                     AILGDIYYSYSTQAGKDAAAQALARAKAGNPLAVSVEHGGGEKYNPKTKRMEATDLWF
                     GGVASVVRGACERCVMPRANEADADGTTGDESMDEETQKALSALEARLKACEEKLAAL
                     TEGPPAEVAEEVAEMGKKLGAALEQLKELEGVKMKNATYEERIKALEKTPNRRTVAEQ
                     ERELEQLTVPDGYSVRRV"
     gene            4195..4662
                     /locus_tag="PLJ11_03540"
     CDS             4195..4662
                     /locus_tag="PLJ11_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03773.1"
                     /translation="MADISKFPDMGDNILVSGDNIQTYVAGAAIKKGQAVAIHATGVS
                     MTVHPAVKGTTASVEGVALATVDSGENVAVAGPGCVVYMANADGTTAIDAGDGVEDND
                     NAVGGTISALPANSGGVTGAYANLVGVALDDIAGGGTGRVRLICSITCIPNSS"
     gene            4672..5649
                     /locus_tag="PLJ11_03545"
     CDS             4672..5649
                     /locus_tag="PLJ11_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03774.1"
                     /translation="MTQLLKDYLAVALSDNKATARRLAERPYIRKLGYLDEQGRVQPV
                     RELLLTGDLSGSNLIQTEVKATVVEGAQPVRAFMDALPIDRTPGTTFKWPYGASGVYA
                     KKYPQGAEIHINTQEYKAASYDASEVIAQRPLITDTMIESGQVDIIEQEIKYAGAAVM
                     NKAERICLSAILENSGLEWDTTGSNQGLKAVAKAVSKLKGVNMAPDVVVMHPDAEGIC
                     MQDVVVPTSPGADTIARGQGIPDGYLGLKWRVCSVADDSSTYTWGYSADGNIGMLVLD
                     SARAGGIYLPRDLTVKDYEDPIRDMKGMTVTMRMDCQSHIANGACRVEY"
     gene            5685..5942
                     /locus_tag="PLJ11_03550"
     CDS             5685..5942
                     /locus_tag="PLJ11_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03775.1"
                     /translation="MLTENGSGKYLNQQWRRKRAQAMVDRDRFSDSELEWLEIGENDG
                     TGKLRKETYEIETQPVVDLPYAATSRRRHDVRERPECGGID"
     gene            5942..6331
                     /locus_tag="PLJ11_03555"
     CDS             5942..6331
                     /locus_tag="PLJ11_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03776.1"
                     /translation="MGQRFTQSNLNMSGYETDVDLTAGQNYTTAGSSIPVDGVDEVLV
                     FAKGTGANAASSGTVTFYVAYSYDGTTFTTESDAIVMTLNANNPVRAKPVSLWIKGCK
                     ALKVIRMVNGDVTYGVNDVNAQFVSQG"
     gene            6336..6707
                     /locus_tag="PLJ11_03560"
     CDS             6336..6707
                     /locus_tag="PLJ11_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03777.1"
                     /translation="MSYCSTVDLVNATGAAYDTTTLQALIDRADRQIDARLRAARVAG
                     SGDAIKEASLSLSIAMLLTRMRMDGTKPASLSLGGLSMSDNIDQAIKELQDRAWELVD
                     EHIDHFSPSRRYYVARSDGRR"
     gene            6712..7029
                     /locus_tag="PLJ11_03565"
     CDS             6712..7029
                     /locus_tag="PLJ11_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03778.1"
                     /translation="MDTTTKILIGAAAVAVISYAGTQLMMRFPGARAWLETKLIDAYA
                     YLDKHKDEVPDEAKGVWTAAYEFCDSTLQAMADGTLTWAESRKAGLQIISLMNEVRKI
                     IKV"
BASE COUNT         1526 a         2055 c         2415 g         1056 t
ORIGIN      
        1 gacgaggcgg cgggaagacg cgggcaggga gcgaatgggt gctgcagcgc gtgcgggatg
       61 gctatcggag catcgccctg gtcggcaaga cagcggccga cgtccgggat acgatgatcg
      121 aactggggga gtcgtcgatc atgaaggtgg cgcgtccgca cgaacgaccg ctctacgagc
      181 cgtcgaagcg ccgcctagtg ttccctaacg gggccgtggc cgtcgcctac aatggcgacg
      241 agccggacca gctcagaggt ccccagcacg acagcgcctg gatcgacgaa ctggccaagt
      301 tccgttatcc cgaggaatgc tgggacaatc tcatgttcgg tctgcggctc ggtccgaacc
      361 cgcaggcgtt cgttacgacg accccgaggc cgatcccgat catccgcaag ctgatcgcgg
      421 acaaggacac tatcgacgtg cggttcagca cattcgataa cgccgagaat ctctcaccta
      481 tattcctgca gcgggtccgg gagcgatacg aagggacgcg gctcggacgg caggagctgt
      541 acggagagat cctcgacgac aaccccgggg cgctgtggaa gcgcgacgtc atcgagaacc
      601 tgcgggtccg ggagcatccg ccgctggtcc ggatcattgt ggccgtggac ccggcggtca
      661 cgcacaacga ggacagcgac gagaccggga tcgtcacggc gggcatcgcc gcctcggggc
      721 attgctacgt cctcgacgac agctccttgc agggcagtgc taatcaatgg gcagtggcgg
      781 cggtccgcgc gtatcacaaa tgggaggcgg accgcatcgt cggcgaggtc aacaacggcg
      841 gggacctggt cgaggtcaac atccgcacgg tggacccggg cgtgccctac gaggcggtga
      901 ctgcgtcgcg gggcaagctc atccgagccg agccggtgtc cgccctgtac gagcagggca
      961 aggtccatca cgtcggcacg ttcccgcagc tcgaggacca gatggtcgaa tgggtccccg
     1021 gcgagaggag cccggaccgc atggacgcgt tggtgtgggc gatcaccaag ctcatcccgg
     1081 cggcgcagac ggccgatccg agcaggttcc tgcgctacgc ggtcagatga tcgaagcgat
     1141 gtcggatgat ggacggacag gtcctcgatg gatatggcgt ttatattaat gcacgcacct
     1201 aaacatgata atgcggggct cctcagggcc gtacagccca tcatttttcg acagggtgtg
     1261 gatcaggctc aacgagatcg ttaatccacc ggttcggccg cggaccatcg ccggaacgcg
     1321 caggcagagg accggcgccg atctgttctc caagcgggag cggtcctata aggagcttga
     1381 tgccctcgag gtccgatatc tgcagggcgg tccggtgcgc gaggcgatcg acagctatcc
     1441 cctgttcgcc ttgagcaatg gttggtacct cgatgggaag gacgagatgc tggtgaagga
     1501 caccgaggcg cagctggcag ccctggacat agagagcagc atctggcagg ggatcataga
     1561 cagcctggtg ttcggcgatg ccttccagga gatcgtcacc ggaagggggc aatattcgga
     1621 tacggtcctg gcgctccagc cccgaccggc caagatgttc gacatagacg cggacgagta
     1681 cggcgtcaag acagggtaca ggcaatatct ggaccaagaa ggtagaaaat acatcccgct
     1741 agccctcgac cagatcctgc atatatccct gttccacatc ggcgggtccg tgtacgggac
     1801 atcgctggtg aacagcgcca aggacgacat cgatcgggac gtacagatga tcgaatccct
     1861 tgtcagcgcc atcgagcgcc acgggcaccc gcgatatcat gccaaggtcg gaaaggaggg
     1921 ggaggatgtc gggcagccgg tgctggactc cgtggccgac cagctgcacg acctcaagag
     1981 caacacggag ctagccactt gtcatgatgt ggagatcgtg tccctggaca ccgccggggt
     2041 cggcaacact caagcctatt cggacctcac cctcgggcgc atggcctgcg cattgggtgt
     2101 tcctcttgac gtcttgggcg tcaccgaggg cagcaacagg tccacggcca ccgtgcggca
     2161 gaaggtcttc gagacgaaga tctcaacgat ccagcgccgg atcgagaaca tatacaactc
     2221 gaatataatc gatcgcctca ccggccagcc aggctctgta aggttcaagt tcaacgacat
     2281 atccccggag gacgagcttc gcgaggtcga atacgtcgcc aaggtcctgg ccgcggatcc
     2341 gatcagcccg atcgtcggcc atgagtgggc gaggcgcagg ctgcggatcc ctgaggagga
     2401 tgcgggacca tgacctcccc aggaaggata gacccgcgcg acccgtcccg cctgagggcg
     2461 accgaggagc gccgcgcccg catcgccgag cgggacatca aggaagcggt cgacgcggcg
     2521 gtcaagcggg tgatcgaggg caacatcaca gatcccaaga ccattcaggc gatcgtgcag
     2581 gaggaattgg gccgctacga gtccgtcgcc tccgatagat atcggtcgtg gacatcggat
     2641 acggtgcaga ggtccgtcct ccgcgccgag cagctcctgc gcgcatcggg agtgcgcatc
     2701 gacgctgcgt tcggtccgcg ccctatctcc ccgcagctcc gcgacgcgct gacgctcaac
     2761 gtgcagaacc agatcgagtc gctcaccgcc tcgacgaagg ccaaggtcac aagcgccttg
     2821 atcgagagca tgagcgctgg cgacggcgcg aaggccgcgg ccaagcgggt gagcgaggcc
     2881 acgggcatgg agcgcagcag ggcgtccttg atcgcccgga cggagacgat gagagcctat
     2941 aaccaggtca acgaggacca gttccggaag cacgacatcg cggccgtgga atggctggcg
     3001 gcgatcgatg agcggacctg cgacgtgtgc ggcgcgcatc acggtcagcg gtactcgatc
     3061 gacaagcatc cagagatccc ggcgcatccg aactgccggt gcatactggt cccggtcatt
     3121 gagggggcgg gctgatgcct tacaggacga tagcggacct ccctgagaac atccgcaaca
     3181 gcctgaccga ggaggaggcg aagcggttca tcgccgcgtt caacgcggcc tacaaggacg
     3241 gcaaggacga gaggacatgc ttcaagatcg ccatgaccgc cgcgaagaag cgagaggcat
     3301 ccgcagcaca tagtgccatg aggatgctga accggaccct ttcgttcagc aattacattg
     3361 aggtcgatgg cgggatgctc gtcaaggacg tcccgctgct ggcggtcggt gaatggaccg
     3421 attcggcggt ggggaccccg ctctactatc cggccaaggt cctggagagg gacgctctga
     3481 actggtatga caacagcttc tgggccaggc acggcgggaa ggttccgcgc agcgtcgtct
     3541 ccgatctgat cggcgaggtc cgcaacatac gctacgacgg atcgtacaag gagaacggca
     3601 tggtggagcc gggcgcgatc ctcggggata tttactactc atactccaca caggcaggga
     3661 aggacgcggc ggcgcaagcg ctcgcacgcg ccaaggcagg caacccgctc gccgtgagcg
     3721 tggagcacgg cgggggggag aaatacaatc ccaagaccaa gcgcatggag gccacggacc
     3781 tgtggttcgg tggcgtcgcc agcgtggtca ggggagcgtg cgagcggtgc gtgatgccgc
     3841 gtgcgaacga ggcggacgcc gacgggacga caggagacga aagcatggac gaagagacgc
     3901 agaaagccct ctccgcattg gaggccaggc tcaaggcgtg cgaggagaag ctcgccgccc
     3961 tgaccgaggg accgccggcg gaggtggccg aggaagttgc cgaaatgggc aagaagctgg
     4021 gcgcagcgct cgagcagctc aaggagctgg agggcgtcaa gatgaagaat gcaacttatg
     4081 aggagcgcat caaggcgctc gagaagacgc cgaaccgcag gaccgtggct gagcaggaga
     4141 gggaactcga acagctcacc gtgcccgacg ggtacagcgt gaggagggtg tgagatggca
     4201 gacatatcga agttcccgga catgggcgac aacatcctgg tgtccgggga caacatacag
     4261 acgtacgtcg caggagcggc gatcaagaag gggcaggccg tggcgatcca tgccacgggc
     4321 gtgagcatga ccgtgcatcc ggcggtcaag gggaccaccg cgagcgtgga gggggtcgcc
     4381 ctggcgaccg tggactcggg cgagaacgtg gccgtggccg gcccgggctg cgtcgtctac
     4441 atggcaaacg cggacggcac caccgccatc gacgccggtg acggggtcga agacaatgac
     4501 aacgccgtgg gtgggaccat aagcgcgctg ccggcgaaca gcggtggagt gactggggcc
     4561 tacgcgaacc tggtgggcgt ggcgttggac gacatcgccg gcggagggac cggcagggtg
     4621 aggctgatct gttcgatcac ctgcatcccg aactcgagct gaggtgaaca aatgacacaa
     4681 ctactcaagg actatctggc cgtggcgctt agcgacaaca aggcaacggc caggcggctc
     4741 gccgagcggc catacatacg caagctcgga tatctggatg aacagggcag ggtccagccg
     4801 gtgagggagc tgctgctcac gggcgacctg agcggcagca acctgatcca gacggaggtc
     4861 aaggccaccg tcgtggaggg cgcgcagccg gtcagggcgt tcatggatgc cctcccgatc
     4921 gaccggaccc ccgggaccac gttcaagtgg ccgtacgggg ccagcggcgt ctacgcgaag
     4981 aagtacccgc agggcgcgga gatacacatc aacacgcagg agtataaggc cgcatcctac
     5041 gacgccagcg aggtcatcgc tcagaggccg ctcatcaccg acaccatgat cgagtcgggc
     5101 caggtggaca tcatcgagca ggagatcaag tacgccggtg cggcggtcat gaacaaggcc
     5161 gagcgcatct gcctctccgc gatcctggag aacagcgggc tggagtggga caccacgggg
     5221 tccaaccagg gcctcaaggc cgttgccaag gccgtctcca agctgaaggg ggtgaacatg
     5281 gcgcccgatg tggtcgtcat gcatcccgac gccgaaggca tctgcatgca ggacgtggtc
     5341 gtcccgacct cgcccggagc ggacacgatc gccagagggc aggggatccc ggacgggtat
     5401 ctcggtctga agtggcgcgt atgcagcgtc gcggatgact ccagcaccta cacctgggga
     5461 tactccgccg acggcaacat cggcatgctc gtgctcgaca gcgcgcgggc gggcggcatc
     5521 tacctgccca gggacctcac cgtcaaggac tacgaggacc ccatccgcga catgaagggc
     5581 atgaccgtga ccatgcgcat ggactgccag agccacatcg ccaacggcgc gtgccgggtc
     5641 gagtactaaa ccccttcccc ttataatttt caggggtgac aacaatgctg accgagaacg
     5701 ggagcgggaa gtatctgaat cagcaatggc ggcgcaagcg agcacaggct atggtcgatc
     5761 gcgatcgatt ctcggattcc gagcttgaat ggcttgagat cggggagaac gacggcaccg
     5821 ggaagctgcg gaaagagacc tacgagatcg agacgcagcc cgtcgtcgat ctcccgtacg
     5881 ctgcgacgtc ccggcgcagg catgacgtcc gggaaagacc ggaatgcgga gggatagact
     5941 gatgggacag agattcaccc aatccaatct gaacatgagc ggttacgaga cggacgtcga
     6001 cctgaccgcc gggcagaact acacgactgc gggatcgagc atccccgtgg acggcgtcga
     6061 tgaggtgctg gttttcgcca agggcaccgg agcgaatgca gccagcagcg ggaccgtgac
     6121 gttctacgtc gcctacagct acgacggcac cacgttcacg acagagagcg atgcgatcgt
     6181 gatgacgctt aacgcgaaca atccggtccg ggccaagccc gtgagcctgt ggatcaaggg
     6241 ctgcaaggcc ctcaaggtga tccgcatggt gaatggcgac gtcacctacg gcgtcaatga
     6301 tgtgaacgca cagttcgtga gccagggatg atagcatgtc atactgcagc accgtcgatc
     6361 tggtgaatgc cacgggggcg gcgtacgata cgacgacctt gcaggcgctc atcgatcggg
     6421 cggaccggca gatcgacgcg cggctcaggg ctgcgcgcgt tgcgggcagc ggggacgcca
     6481 tcaaggaggc gtccctctcg ctctcgatcg ccatgctgct aaccaggatg cgtatggacg
     6541 ggaccaagcc cgcctcgctg agcctgggcg gactgagcat gagcgacaac atcgaccagg
     6601 ccatcaagga gctgcaggac agggcctggg agctggtgga tgagcacatc gaccactttt
     6661 cgccgagcag gcgatattac gtcgcgagga gcgacggtag gagatgagaa catggacaca
     6721 actactaaga tcttgatcgg agcggccgcg gtggccgtca tatcgtatgc aggaacgcaa
     6781 ttgatgatga ggttccccgg ggccagggca tggctcgaga cgaagctgat cgacgcttac
     6841 gcgtacctag acaagcacaa ggatgaggtt ccggacgagg cgaagggcgt ctggaccgct
     6901 gcgtatgagt tctgcgacag cacgctgcag gcaatggcgg acgggacctt gacctgggcc
     6961 gagagccgga aggccgggct gcagatcatc agcctgatga atgaggtccg gaagatcatc
     7021 aaggtctgag gtgagcgggt ggggttcggc ga
//
LOCUS       DAOSYN010000056         6947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_9235_length_6947_cov_4.602002, whole genome shotgun sequence.
ACCESSION   DAOSYN010000056 DAOSYN010000000
VERSION     DAOSYN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9235_length_6947_cov_4.602002"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLJ11_03570"
     CDS             complement(<1..278)
                     /locus_tag="PLJ11_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178753.1"
                     /note="required for the ATP-dependent degradation of
                     proteins by the 20S proteasome; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HOO03779.1"
                     /translation="MDEELDQSISNEIVEKLELLERQNSELLEEVRRVEGEKRYVESE
                     LFRLQKEIKRMRSELERLKSPPLIIGSIKDMLVDGRVIVKSSTGPDFIV"
     gene            complement(400..1125)
                     /locus_tag="PLJ11_03575"
     CDS             complement(400..1125)
                     /locus_tag="PLJ11_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOO03780.1"
                     /translation="MKTLRQKCTAELIGTMFLVIVAIASTILPITKGVDLGVTVFVNA
                     VAVAFVLFALIEALAPISGAHFNPAVTVVLAQTGEMRKGEAPYYIASQFAGAFLGLIT
                     VHLMFWDSNPVLVTVSENVMTPSQYFAEVVGTFILVGVIYGCVRGKSTATGMAVAFLV
                     GGMLVSTASTMFANPAVTFARMMTYAICGIAPASGVVFMAMEVIGAMLAMVVFAKVLY
                     PSRLEGKEICEPGYCPPKLIEIK"
     gene            1248..2312
                     /locus_tag="PLJ11_03580"
     CDS             1248..2312
                     /locus_tag="PLJ11_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO03781.1"
                     /translation="MKVRMVIIGGFLGAGKTTLITRLARDLKDAGVNVGIIMNDQADT
                     LVDTQYAQALGLNSCEVAGGCFCCRFPDLMGQAERLVESSRPQVVMAEPVGSCTDLLA
                     TVVAPLKAFYPERFQVAPLMVMVDAERAIQEGFDRQTLNGYLRRHQLEEAEVVILSKA
                     DLVGREDIERLKGTIAGINPRAITIVYSAVSSEGYGKVLDVVRSEEVSENGPKEIDYA
                     VYAEAEAELGWYNGTLRADFGEGVDSHALGMAILGNISSQYPMEDIAHAKVMVTSLSN
                     AMKMSCVRGRVTVDLARGSRVAKGPGRITVNARVVSDPGRLKGNVRAALEGALPGKRL
                     EFEREDCFSPAPPKPFHRMT"
     gene            2410..2901
                     /locus_tag="PLJ11_03585"
     CDS             2410..2901
                     /locus_tag="PLJ11_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HOO03782.1"
                     /translation="MMCEMCGKEMASLMQVRIEGSVLNVCKSCARFGDNVKAGGKQGA
                     VAEPSVIQARLQNRERRMKTKDVYEGGEESFELAEDFPRRIKEARERLGWKQEELAAK
                     MNERVSIVQKLEGGSMHPDDTLVRKVERTLDIKLKEKVTIAPVEAAPGKKGLTLEDFI
                     KKK"
     gene            complement(3096..3647)
                     /locus_tag="PLJ11_03590"
     CDS             complement(3096..3647)
                     /locus_tag="PLJ11_03590"
                     /inference="COORDINATES: protein motif:HMM:NF021513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HOO03783.1"
                     /translation="MSELQRTVAVVFNRKGKKLLSEREFINALFFELKWADPTGTGRI
                     EAKEAQRILEAAIRKGLLELSDGYVRPAFDHRSVDVPLNYRPTRDMLKELDDGPSKPS
                     SPPSPSAQGKSEAGLMPVFTVLIDEIAAKSGWSKRDVVARINRVVERLSVDAEVAALL
                     IGRDVGLDVGKYILDVKEEVLRK"
     gene            3886..5520
                     /gene="thsB"
                     /locus_tag="PLJ11_03595"
     CDS             3886..5520
                     /gene="thsB"
                     /locus_tag="PLJ11_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HOO03784.1"
                     /translation="MAMGNAPILILKEGSKREKGKDAQYNNIMAARAIADAVRSTLGP
                     RGMDKMLVDSMGDVVITNDGVTILKEIDVEHPAAKMLVEVAKTQDEECGDGTTTAVIL
                     TGELLKKAIDLIDANIHPTIITSGYRMACNKAMEIIDSMAIKVEPKDRDTLMKIAKTA
                     MMSKSVSGSKELMAQVAVDSVINVAEKVNGKWKVDMDNIQVTKKHGGSMDDTQLIAGI
                     IVDKEPVHPGMPKKIEKAKIALLDVALEIKKTEIDAKIEITDPTQMHAFLDEEERMLK
                     GMVDKIKAVGANVVFCQKGIDDLVQHFLAKEGIYAARRVKKGDMEKLSKATGATIISK
                     INELDPNDLGKADLVEVRKIQEEEMTFVTGCKNPKAISILIRGGTEHVVAEIERSLDD
                     AMSVVALAIEDGRMLTGGGSTAVEVALRLREYSASVGGREQIAIDAFASSMEIVPTAL
                     AENAGLDPIDILIEMRKAHKANKKYAGVNVFTGKVEDMVANDVLEPFRVGKQAINSAT
                     DAAVMILRIDDVIASKGTGGPSPKDQVGKEGLADVD"
     gene            5675..6139
                     /locus_tag="PLJ11_03600"
     CDS             5675..6139
                     /locus_tag="PLJ11_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOO03785.1"
                     /translation="MARYKAEAEEAKAEAERNLDLAKRIQAEFDNYKKRVQRDREEQV
                     RAANDKLLCELLGFLDDFERALSSNASPNEFRRGVESIHANLRSLLQSKGLVETPCET
                     FDPALHEALCVGEGEEGKVLEVYQKGYRLGPRVVRYAKVKVGNGTERGESDG"
     gene            6132..>6947
                     /locus_tag="PLJ11_03605"
     CDS             6132..>6947
                     /locus_tag="PLJ11_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO03786.1"
                     /translation="MAKIIGIDLGTSNSAAAVMEGGRPTIIPSAEGTSIGGKAFPSYV
                     AFTKDGQLLVGEPARRQAVTNPEGTIVAIKRKMGTDYKVKVNGKEYTPQQISAFILQK
                     IKRDAEAYLGEEVNKAVITVPAYFNDNQRQATKDAGAIAGLEVIRIINEPTAAALAFG
                     LDKVGKSQKIMVFDLGGGTLDVTIMDFSEGVFEVVATSGDTQLGGTDMDEILIKYAVD
                     NFRKETGVDLTSDKMAMWRVREACEKAKIELSTTMSTEINLPFIAAGADGPKHL"
BASE COUNT         1539 a         2027 c         2213 g         1168 t
ORIGIN      
        1 acgatgaagt ccgggcccgt gctgctcttg actatgaccc ggccgtccac aagcatgtcc
       61 ttgatgctgc caatgataag gggcggggac ttgaggcgct ccagctcgga gcgcatgcgc
      121 ttgatctcct tctggagccg gaagagctcg ctttcgacgt acctcttctc gccctccacc
      181 ctcctgacct cctccagaag ctcactgttc tggcgctcca gcagctcaag cttctcgacg
      241 atctcgttgg agatggattg gtcaagctct tcgtccaaca aatgtggcac cttcgagcat
      301 tgattaaacc gtcctctata ttagcgtttg ccaatgccta tccgcgttcg ctaccgaggc
      361 gcatcgcgca tccattggat gacccgccca ccgcccggct catttgatct cgatgagctt
      421 cggagggcag tatcccggtt cgcagatctc cttcccctcc agccgcgagg ggtacagcac
      481 cttggcgaag accaccatgg ccagcatggc cccgatgacc tccatggcca tgaacaccac
      541 cccgctggcc ggggctatgc cgcagatggc ataggtcatc atccgggcga aggtgacggc
      601 cgggttggcg aacatggtgc tcgccgtcga gaccagcatc cccccgacca ggaaggccac
      661 ggccatcccc gtggcggtgc tcttcccccg cacgcacccg tagatcaccc ccacaaggat
      721 gaaggtgccc accacctcgg cgaagtattg gctgggggtc atgacgttct cggaaacggt
      781 caccaggacg gggttcgagt cccagaacat aaggtgcacc gtgatgaggc ccaggaacgc
      841 ccccgcgaac tgggaggcga tgtaataggg cgcctctccc ttgcgcatct ccccggtctg
      901 cgccagcacc acggtcacag cgggattgaa atgggccccg gagatgggcg ccaaggcctc
      961 tatcagggcg aagaggacga aggccaccgc caccgcgttg acgaagaccg tcacccccag
     1021 gtcgaccccc ttggtgatgg ggaggatcgt gctggcaatg gccacgatga ccaggaacat
     1081 ggtgccgatc agctcggccg tgcacttctg tctgagcgtc ttcatggaac gacctgagag
     1141 gaaatgttga tttcatttat ttataattga ttttaacatc aaataattaa atgttaaata
     1201 cataagcaat ggttatcggt atgtaaaaaa ggctgcgagg ggcgcggatg aaggtacgga
     1261 tggtcatcat cgggggcttc ctgggggctg gaaagacgac gctgatcaca agattggcca
     1321 gagacctcaa ggacgcgggc gtgaacgtcg gcatcatcat gaacgaccag gcggacaccc
     1381 tggtcgacac ccaatacgcc caggccctgg gcttgaactc ctgcgaggtg gccggggggt
     1441 gcttctgctg ccggttccca gatctcatgg gccaggcgga gaggctcgtc gaaagctcga
     1501 ggcctcaggt cgttatggcg gaaccggttg gaagctgcac cgacctcctg gccacggtgg
     1561 tggctccctt gaaggcattc taccccgaga ggttccaggt ggcgccgttg atggtaatgg
     1621 tggacgccga gcgcgccatc caagagggct tcgatcggca gacgttgaac gggtatctca
     1681 gaaggcatca gctcgaggag gccgaggtgg tcatcctgtc caaggccgac ctggtcggca
     1741 gagaggacat cgaacgcctg aagggaacga tcgccgggat caatcccagg gctatcacca
     1801 tagtctattc cgcggtgagc tcggagggct acgggaaggt gctagacgtc gtaaggtcgg
     1861 aggaggtgag cgagaacggg cccaaggaga tcgactacgc cgtatacgcc gaggcagagg
     1921 ccgagcttgg atggtacaat ggcacgctga gggccgattt cggggagggt gtcgactccc
     1981 acgccttggg catggccata cttgggaaca tctcctccca atacccgatg gaggacatcg
     2041 ctcacgctaa ggtgatggtc acctcgctca gcaacgccat gaagatgtcc tgtgtccgag
     2101 gaagggtgac cgtggacctg gccaggggct cccgcgtcgc caagggcccc gggaggatca
     2161 ccgtgaacgc ccgggtggta tccgacccgg gaaggctcaa agggaacgtg cgggccgcgt
     2221 tggagggggc gctgccgggg aagaggctcg agttcgagcg cgaggactgc ttctcccccg
     2281 ccccgcccaa gcccttccat cggatgacat gagcggaccg aacgatgtcc agaccatcta
     2341 tggacccctt ctgggacccc ttctgctcac atttaaatta cccttcctat attagctttg
     2401 acgatagcga tgatgtgtga gatgtgcgga aaggagatgg cgagcctgat gcaggtgagg
     2461 atcgaggggt cggtcctcaa tgtgtgcaag agctgcgccc ggttcgggga caacgtcaag
     2521 gcgggtggga agcagggggc ggtcgccgag ccatccgtca tccaggcccg cctgcagaac
     2581 cgcgagcgcc gcatgaagac caaggacgtc tacgaaggcg gggaggagtc ctttgagctg
     2641 gcggaggact tccccaggag gatcaaggag gcccgggaac ggctcggctg gaaacaagag
     2701 gagctggcgg ccaagatgaa cgagagggta agcatcgtcc aaaagctgga gggtgggagc
     2761 atgcatcccg acgacacgct tgtccgaaag gtggagagga ccctggacat caagctcaag
     2821 gagaaggtca ccatcgcgcc ggtggaggcg gccccaggca agaaaggact gaccctcgag
     2881 gacttcatca agaagaagtg agcgcgccat ccttttttct gcctacggga tccagaggac
     2941 ccgttagcca tccctgcacc ttcacgaccc atgagggacg cagggcatta ccagccctct
     3001 tacgaacggc gcccctgtga gaaccgaggg gagccaacca catttcctag cattaccgta
     3061 ggatgggcag tgcgtcctat tgggccaggt ggccgtcact tgcgcaggac ctcctccttc
     3121 acatcgagga tgtacttccc gacgtccaag ccgacatccc gtcctattag cagtgcggcc
     3181 acctcggcgt cgaccgacag cctttccacg accctgttga tgcgcgcgac gacatcccgt
     3241 ttgctccatc ccgacttggc cgctatttcg tcgataagga cggtgaagac aggcatcagc
     3301 cctgcctcgc tctttccctg ggctgagggc gatggggggg aggacggctt ggatgggccg
     3361 tcatcaagct ccttcaacat gtcccttgtc ggcctgtagt tgagggggac gtccaccgac
     3421 ctgtggtcga acgccggccg gacgtagccg tccgagagct ccaggagccc cttcctgatc
     3481 gccgcctcca ggatgcgctg ggcctccttg gcctcgatcc ttcccgtacc ggtggggtcc
     3541 gcccacttca gctcgaagaa gagggcgttg atgaactccc gctcggacag cagcttcttg
     3601 cccttgcggt tgaagacgac ggcgacggtc cgctggagtt cgctcatacc cgatcgctcc
     3661 cacgaatcgc atcgggggat ataaccttgc cctacgccag gggggaataa tctcaaatac
     3721 ggtccagcct ttatgttacc atgcgctccc ggacatggat catggggttt tcatatgggt
     3781 gcggagcgca tatacaatat gtatatatag aagaacaaac attccaagcc cgctagcata
     3841 cagctaggtg tcaatctgag ttgataccga gggaggttag tagctatggc aatgggaaac
     3901 gcacctatac tgatacttaa ggaaggttcc aagcgtgaga agggcaagga cgcccaatac
     3961 aacaacataa tggccgcaag ggccatcgct gacgctgtta ggagcaccct agggccccgc
     4021 ggaatggaca agatgctcgt cgactccatg ggcgacgtcg tcatcaccaa cgacggcgtc
     4081 accatcttga aagagatcga cgtcgagcac cccgccgcca agatgctcgt ggaggtcgcc
     4141 aagacccagg acgaggagtg cggggatggg accaccaccg cggtgatcct caccggcgag
     4201 ctgttgaaga aggccatcga cctcatcgat gccaacatcc acccgaccat catcacctcc
     4261 gggtaccgca tggcctgcaa caaggccatg gagatcatcg attccatggc gatcaaggtc
     4321 gagcccaagg accgcgatac ccttatgaag atcgcgaaga ccgccatgat gagcaagtcg
     4381 gtctccggct ccaaggagct gatggcccag gtggcagtcg actccgtgat caacgtggcg
     4441 gagaaggtga acggcaagtg gaaggtcgac atggacaaca tccaggtcac caagaagcac
     4501 gggggctcca tggacgacac ccagctcatc gctggcatca tcgtggacaa ggagccggtg
     4561 catcccggca tgcctaagaa gatcgagaag gccaagatcg ccctgctgga cgtggcgctc
     4621 gagatcaaga agaccgagat cgacgccaag atcgagatca ccgaccccac ccagatgcac
     4681 gccttcctgg atgaggagga gcgcatgctc aagggcatgg tcgacaagat caaggccgtg
     4741 ggcgcgaacg tcgtgttctg ccagaagggc atcgacgacc tggtgcagca cttccttgcg
     4801 aaggagggca tctacgccgc ccgccgcgtg aagaagggcg acatggagaa gctgtccaag
     4861 gccacgggag cgaccatcat ctccaagatc aacgagctgg accccaacga cctcgggaag
     4921 gccgacctgg tcgaggtccg caagatccag gaggaggaga tgaccttcgt gacgggctgc
     4981 aagaacccca aggccatatc catcctgatc cggggcggga ccgagcacgt ggtcgccgag
     5041 atcgagaggt ccctggacga tgccatgagc gtcgtcgctc tggccatcga ggacgggcgc
     5101 atgctgaccg gcggcggctc caccgctgtc gaggtcgccc tgcgcctgcg ggagtactcc
     5161 gcctccgtcg gagggcgcga acagatcgcc atcgacgcct tcgcctcctc catggagatc
     5221 gtgcccaccg ccctggccga gaacgccggg ctggacccga tcgacatcct catcgagatg
     5281 aggaaggccc acaaggcgaa caagaagtac gccggggtga acgtcttcac cggcaaggtc
     5341 gaggacatgg tggccaacga tgtcttggaa ccgttcaggg tcgggaagca ggcgatcaac
     5401 tccgccaccg atgccgcggt gatgatcctg aggatcgatg acgtcatcgc ctccaagggc
     5461 acgggcggcc cgagcccgaa ggaccaggtc ggcaaggaag gccttgcaga cgtcgattaa
     5521 acgctaaagg ggagggggcg tcccctccct gaaaaaatcc tttaggttct gatcgatagc
     5581 taattggtgg gcccagatgg aaaacggttc caatggaaca tggcccctgc aaaaggggga
     5641 ggctcaaggc gagccgtcaa ggccagagga cgaggtggcc cgatacaagg ccgaggcgga
     5701 ggaggccaag gcggaggcgg agaggaacct ggacctggcc aagaggatcc aggccgagtt
     5761 cgataactac aagaagcgcg tccagcggga cagggaggag caggtgaggg cggccaatga
     5821 caagctgctc tgcgagctcc taggcttcct ggacgatttc gagcgggcgc tcagcagcaa
     5881 tgccagcccc aatgagttca ggcgcggggt ggagtccatc cacgccaact tacgatcatt
     5941 gctccagtcc aagggactgg tggagacgcc ctgcgagacc ttcgacccgg cgctgcacga
     6001 ggcgctgtgc gtcggggaag gggaggaggg caaggtcctg gaggtgtatc agaaaggata
     6061 tcgcctggga ccaagggtcg tgcggtatgc caaggtgaaa gttggcaatg gcacggaaag
     6121 aggtgagtca gatggctaag atcataggaa tagatttagg cacaagcaac tcggccgcgg
     6181 cggtcatgga gggcggccgg ccgacgatca tcccgagcgc ggaggggacc agcattggcg
     6241 ggaaggcctt cccctcctac gtcgccttca ccaaggacgg gcagctcctg gtcggcgagc
     6301 ctgcccgtcg gcaggcggtt acgaacccgg aaggaacgat cgtggcaata aagcgcaaga
     6361 tgggcacgga ctacaaggtc aaggtcaatg ggaaggagta cacgccacag cagatatccg
     6421 ccttcatcct tcagaagata aagagggatg ccgaggcgta cctcggcgag gaggtcaaca
     6481 aggccgtcat caccgtgccc gcctacttca acgacaacca gaggcaggcc accaaggatg
     6541 cgggggccat cgccgggctg gaggtcatca ggatcatcaa cgagccgacc gcggccgcgc
     6601 tcgcgttcgg cctggacaag gtggggaagt cccaaaagat catggtcttc gacctgggcg
     6661 ggggaacgtt ggacgtcacc atcatggact tcagcgaggg ggtcttcgag gtggtggcca
     6721 ccagcgggga cacccagctg ggcgggacgg acatggatga gatcctcatc aagtacgcgg
     6781 tcgacaactt caggaaggag accggggtgg acctcacctc cgacaagatg gccatgtgga
     6841 gggtgcgcga ggcctgcgag aaggccaaga tcgagctgtc caccaccatg tccaccgaga
     6901 tcaacctgcc gttcatagcc gcgggcgccg acggccccaa gcacctg
//
LOCUS       DAOSYN010000057         6864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_9405_length_6864_cov_4.206638, whole genome shotgun sequence.
ACCESSION   DAOSYN010000057 DAOSYN010000000
VERSION     DAOSYN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9405_length_6864_cov_4.206638"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="PLJ11_03610"
     CDS             complement(<1..1712)
                     /locus_tag="PLJ11_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO03787.1"
                     /translation="MVSKSGSVLVVGGGIGGVQTSLDLAESGFKVYMVESKASIGGVM
                     SQLDKTFPTNDCSMCILSPKLVEAARHPMISMMTLTEVMGIEGEAGNFTVTLKKKPRY
                     VREDRCVGCGLCAEKCPSKVPSEYELGLMNRKAIYVAYAQAVPMKYAIDADKCLYLTK
                     GKCGNCKKVCPADAIDYEMKEQIEKINVGAVVLAPGYELFDARLKKEYGYKEFKNVLN
                     SLEFERVLSATGPYQGHVVRPSDHQTPKKVAFIQCVGSRDEKVGNPFCSSVCCMYALK
                     QAIIAGEHTTGLKSTIFFMDLRAFGKEFEDYAKRAENEYGIKMHRGTRVASVEETEGN
                     NLLLRYSDGNNVLAEEFDLVVLSAGFQPPSSAKALADRLGFKLNEFGFCQTGVYTPLE
                     TTKKGVYVTGAFSAPKDIPTSVAEASGAAAKAGSHVFNNRVSIDTVVKEVPETDVTGQ
                     EPRIGVFVCDCGINIRGTVDVPSVVEYVKTLPNVVYAAENKYTCSADTQESIKKMIKE
                     HKLNRVVVASCTPRTHEALFQSTIKEAGLNPFLFEMANIRDQCSWIHMHEPEKATEKA
                     KDLTR"
     gene            2386..2901
                     /locus_tag="PLJ11_03615"
     CDS             2386..2901
                     /locus_tag="PLJ11_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03788.1"
                     /translation="MVCQICMGKLKAGLPSVDCECGRTFHITCLKRTGFCPYCQKAYS
                     PEEVEERATYPELESMECPICGRTVLKDSGVCECGAIIADEDGVFYCPICGTQMDEGE
                     VECPYCHELFEEVKLVLCPSCGRMIDEKKGVCECGLFLGEVCPECGVRLSPEDRCCPS
                     CGIAFEMVGDE"
     gene            2985..4034
                     /gene="ftsZ"
                     /locus_tag="PLJ11_03620"
     CDS             2985..4034
                     /gene="ftsZ"
                     /locus_tag="PLJ11_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO03789.1"
                     /translation="MFDDMFQKGAEQPIATSSTEDDELRALVDSLKIKIAIIGCGGGG
                     SNTIRRMYQAGVEGVKLVACNTDARHLLSIQAHHKLLMGRTMTKGLGSGSMPEVGKRA
                     AEESENDLWKYLDGQNIVFVVAGLGGGTGTGSAPVVAELAKRAGALTIGVVTLPFKAE
                     GAMRMSNALKGLERLKEQCDTTIVLQNDRLLELVPKLPLEAAFRVTDEVLMQSIKGIT
                     DALTKPGLINIDFNDLLTIMRNGGMALIGLGESSEYGKRAEECIEEALSSPMLGDVDI
                     KQAKGALVRVIGGEDLTVTEAEKAALLVSERVDPRARIIWGCAVDGNIKDEMRVMVVL
                     TGVRFNSIVDSFKGK"
     gene            4045..4344
                     /locus_tag="PLJ11_03625"
     CDS             4045..4344
                     /locus_tag="PLJ11_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03790.1"
                     /translation="MVVKEKRGRRRYIAFRVDGKASDEELLAALNAALSSRGVKVPKV
                     IQFDGRMGVVRCSPKDKDRILAALAADGKGIVPLSTSGTLLTLRKRYFSGLERRD"
     gene            4438..5160
                     /gene="psmA"
                     /locus_tag="PLJ11_03630"
     CDS             4438..5160
                     /gene="psmA"
                     /locus_tag="PLJ11_03630"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:NF003075.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HOO03791.1"
                     /translation="MQPGQMAYDRAITVFSPDGRLFQVEYAREAVKRGTTTVGLKFKD
                     GVVLIVDKRIASRLMEPKSIEKIFQIDHHIGCATSGLVADARILVDQARVLAQINKIT
                     YDERADVEDLVKRICDYKQNYTQYGGVRPFGTALLVAGVDDQGEHLFETDPSGALVSY
                     KAGSIGAGRNVVMEVFEEEYQEGMSMEDAIILGLKALKKATEEENLNPKAVEIGVVRH
                     GENFHRLDEPDVETYIAKVNQD"
     gene            5174..5869
                     /locus_tag="PLJ11_03635"
     CDS             5174..5869
                     /locus_tag="PLJ11_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HOO03792.1"
                     /translation="MVDLEEAIVARLESHGETFEVLIDPKVVNHLKEGKDVDLIDYMV
                     IDEIFKNARKGTRASEEKLREVFGTTDPVEIAKVIILKGEVQLTAQQRKEMLESKRLR
                     IIATIARNAINPQTGGPHTPARIELAMEEAKVHIDPFKPVDLQVQHVLEKLRPLIPIR
                     FDKVRIAVKLKADEYGRCYEDMKEMGKITREEWQKNGDWIGVVEIPAGLRDDLFQRLN
                     AKTHGNVETKQLK"
     gene            5899..6582
                     /gene="rrp4"
                     /locus_tag="PLJ11_03640"
     CDS             5899..6582
                     /gene="rrp4"
                     /locus_tag="PLJ11_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HOO03793.1"
                     /translation="MSKESTARELVLPGDLLDPGTMKPGEGTYVHDGKIFAAMLGIKS
                     VKSNFVNVIPLGGRYIPAPGDMVIGKVIDIGPSNWLVDINSPYPAPLHVNEVPWRVEF
                     GDTARYLSVGDTLLAKVLSVDETKRVQVTMKEQGLRKLQGGQILEISHSKVPRVIGKN
                     GSMIQMIKNYTNCRIFIGQNGRIWVDGNMDDMVHAMRAIKMIEEGAQTLKLTERVKEF
                     LDSVYRKEE"
     gene            6593..>6864
                     /locus_tag="PLJ11_03645"
     CDS             6593..>6864
                     /locus_tag="PLJ11_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504198.1"
                     /note="involved in the 3' to 5' degradation of a variety
                     of RNA species; forms a trimer of heterodimers (hexamer)
                     with Rrp42; Rrp41 is the catalytically active subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HOO03794.1"
                     /translation="MGGNSDMKLIDENGIRIDGRKVDELRPIRIEAGVLKSADGSAYV
                     EIGKNKVLAAVYGPRECHPRHMQNPAKAIVQCKYNMISFSVSDRKRP"
BASE COUNT         1452 a         1891 c         2161 g         1360 t
ORIGIN      
        1 cgcgtcaggt ccttggcctt ctcggtggcc ttctccggct cgtgcatgtg gatccaggag
       61 cactggtcgc ggatgttggc catctcgaag aggaacgggt tcagcccagc ctcctttatg
      121 gtgctctgga acagggcctc atgcgtccgg ggggtgcagg aggcgaccac tacgcggttc
      181 aatttgtgct cctttatcat cttcttgatg ctttcctggg tatcagcgga gcaggtgtac
      241 ttgttctccg cagcgtacac tacgttgggc agggtcttca cgtactccac gacggagggc
      301 acgtcgacgg tcccgcggat gttgatgcca cagtcgcaca cgaacacacc gatgcggggc
      361 tcctgcccgg tgacatcggt ctcggggacc tccttgacaa cggtgtcgat gctgaccctg
      421 ttgttgaaca cgtgggagcc ggccttggcg gccgcaccgc tcgcctcggc cacggaggtc
      481 gggatatcct taggggcgct gaaagcacca gtgacgtaca cccccttctt ggtggtctca
      541 aggggggtgt agaccccggt ttggcagaag ccgaattcgt tcagcttgaa ccccagccgg
      601 tcagccaagg ccttggcgga tgatggaggc tggaagccag cggacaggac gaccaggtcg
      661 aactcctcgg ccaggacgtt gttcccatcg ctgtagcgca gcagcaggtt gttgccctcg
      721 gtctcctcca cggaggcgac gcgggtgccg cggtgcatct tgatgccata ctcgttctcg
      781 gccctcttgg cgtagtcctc gaactccttg ccgaacgccc tgaggtccat gaagaagatg
      841 gtggatttca ggccagtggt gtgctccccc gcgattatgg cctgcttgag cgcatacatg
      901 cagcagacgg atgagcagaa ggggttgccg accttctcat ccctggaacc gacgcactgg
      961 atgaaagcga ccttcttagg tgtctgatgg tcggagggtc tgaccacgtg cccctgatag
     1021 ggaccggtgg cgctaaggac cctttcgaac tccaggctgt tgaggacgtt cttgaactcc
     1081 ttgtagccgt actccttctt caagcgggcg tcgaatagct cgtacccggg cgcgagcacg
     1141 accgcaccca cgttgatctt ctcgatctgc tccttcatct catagtctat ggcatcggcg
     1201 gggcagacct tcttgcagtt gccgcacttg cccttggtga gatagaggca tttatcagcg
     1261 tcgatcgcgt acttcatggg cacagcctgg gcgtaggcca cgtagatagc cttccggttc
     1321 atcaggccca gctcgtactc gctggggacc ttggacgggc acttctctgc gcagagaccg
     1381 cagccgacgc atctgtcctc gcggacgtac cggggctttt tcttcagggt aacggtgaag
     1441 ttgccggcct ccccctcgat ccccatgacc tcggtcagcg tcatcatgct gatcattggg
     1501 tgcctcgccg cctccacgag cttaggggac agaatgcaca tcgaacaatc gttcgttggg
     1561 aaggtcttgt ccaactggga catgacaccc cctatgctgg ccttgctttc gaccatgtag
     1621 accttgaacc cggactcggc caggtccaaa gaggtctgga cgccgccgat acctcccccg
     1681 acgaccagaa cagaaccgct ctttgatacc atattcgctc ctccttttac gtcatccatc
     1741 gatccttggg acgattgttc ccccttgctg tccttcttga acaatccgcc aaatattgat
     1801 tttaaaccgc tcaacctctc cctcctattg taactcccac tccggaccaa aacgccgggt
     1861 ccgcccctta cccttcgcac acctatccca gagaggcgaa tgggcacctc ccgggaacct
     1921 catcccagag gcctggaccg ccagggagag cccccctgac gactgggcgg ccttgcaggg
     1981 attggaatgt ccatatttca acattatgtg ctctgttgat ggataggaca tggtgttttg
     2041 gtgattgcgt tccgtttaag aacatttcgc ttttatcgtt ttagtaatcg gaagaataag
     2101 cgtgcgtgca taggaaatgg gcttttgacc ttagcgcgct gatcgctccc aatgacggca
     2161 ttcaataacc gggtgggaaa taatataaac ggcgatgcgg tccgttcgag ggtgctcgcc
     2221 ctgttcctcg gatcgctcgg catatccagc ctcatcccta tttattattt tttcgttaaa
     2281 agggaccgag ggcaggcacg gtgctggacc tttcacgagg ataggttgga cccgttaagc
     2341 cccgtgggac ctcgggggaa gatccggcca gtacggggtg agggcgtggt ctgccagata
     2401 tgcatgggaa agctgaaggc cgggcttccc agtgtcgatt gcgagtgcgg aaggaccttt
     2461 cacatcacct gcctgaagcg cactggcttc tgcccctatt gccaaaaggc atactccccg
     2521 gaggaggtgg aggaaagggc gacctaccca gagctggaga gcatggaatg cccgatatgt
     2581 ggtaggacgg tgttaaagga ctccggggta tgcgaatgcg gggcgatcat cgccgatgag
     2641 gacggagtgt tctattgccc catctgcggg acccagatgg acgaagggga ggtggagtgc
     2701 ccttattgtc acgagctgtt tgaggaggtt aagctggtcc tatgcccttc ctgcggaagg
     2761 atgatcgatg agaagaaggg cgtctgcgag tgcggtttgt tccttggaga ggtctgcccc
     2821 gagtgcgggg tccgcttgtc cccggaggac cgctgctgtc cgtcatgcgg catcgccttt
     2881 gagatggtcg gagatgaata gcggccggct gggcttactt tgggcttcct tgccttttaa
     2941 tactatatag aatattcatt acacgggtgg gtgccatttg cccattgttc gatgacatgt
     3001 tccaaaaggg ggccgagcag ccgatcgcaa catccagcac ggaggacgat gagctgcggg
     3061 cccttgtcga ctcgcttaag ataaagatcg ccatcattgg gtgtggagga gggggatcga
     3121 acaccatccg tcgcatgtac caggccggtg tggaaggggt caagctcgtg gcctgcaaca
     3181 ccgatgctcg acatctcctt tcgatccaag cccaccacaa gctcctgatg gggcggacca
     3241 tgacgaaggg gctgggctcc ggttccatgc ctgaggtggg gaaacgggcg gccgaggagt
     3301 cggagaacga cctctggaaa tacctcgacg gacagaacat cgtcttcgtg gtggccgggc
     3361 tgggcggggg gactggcacg ggctccgccc cggtggtcgc agaacttgcc aagagggcgg
     3421 gggccctgac cataggcgtc gttactttgc ccttcaaggc cgaaggcgcc atgcgcatga
     3481 gcaacgccct caaaggattg gagaggctga aggagcagtg cgacaccacc atcgtcctgc
     3541 agaacgatcg gctgttggag cttgttccca agctgccgct ggaggctgcc ttccgggtga
     3601 ccgatgaggt gttgatgcag agcatcaagg gcatcaccga cgccctgacc aagcccggtc
     3661 tgatcaatat cgatttcaac gacctgctca ccatcatgag gaacggcggc atggcactga
     3721 taggcctcgg ggagtccagc gaatacggga agcgggccga ggaatgcatc gaggaggcac
     3781 tttcgtcccc aatgctgggc gatgtggaca taaagcaggc caagggagcc ctcgtacggg
     3841 taatcggggg tgaggacctg acggtcaccg aggcggagaa ggccgcattg ctggtgagcg
     3901 agagggtcga tcccagggcg cgcatcatct ggggctgcgc cgtggacggc aacatcaagg
     3961 acgagatgcg ggtgatggtc gtcctcacag gtgtgaggtt caacagcatc gtcgattcct
     4021 tcaagggcaa atgaggcttc cgggatggtc gtcaaggaga agaggggacg ccgacggtac
     4081 atcgcgttcc gggtggacgg gaaggcctcc gatgaggagc tcctagcggc cctgaacgcc
     4141 gccctgtcct cgcggggcgt gaaggtccct aaggtcattc agttcgacgg gcgcatgggg
     4201 gtcgtccgct gctccccgaa ggacaaggac cggatcctcg ccgcattggc cgccgatgga
     4261 aagggaatag tgcccctcag cacctccgga acgctcctga cgctgcgcaa gaggtacttt
     4321 tcggggttgg aaaggcgcga ttgactggcc ttccctcagg gtttttgaaa agtcccaaga
     4381 aatatatacg tcctcataac ttaggtggct tggatcgagg ataatcggag tgatttcatg
     4441 caaccaggac agatggcata tgatagggct ataacggttt tctcccccga tggccggctc
     4501 tttcaggtag agtacgcccg tgaggctgtc aagcggggca cgaccaccgt cggtctgaag
     4561 ttcaaggatg gcgtggtcct tatcgttgac aagcggatcg ctagccgcct gatggagccc
     4621 aagtccatag agaagatctt ccagatcgac catcacatag ggtgtgccac ctccggcctg
     4681 gtagcggacg cgcgcatcct ggtggaccag gcaagggtcc tggcccagat caacaagatc
     4741 acctatgacg agcgggcgga cgtggaggac ctggtcaaac gaatatgcga ctataagcag
     4801 aactacaccc aatacggtgg ggtcaggcca ttcggcaccg cgcttctggt ggcgggtgtg
     4861 gacgaccagg gcgagcatct gttcgagacg gacccgagcg gggcgctcgt ttcctataag
     4921 gcggggagca taggggcggg gcgcaacgtc gtcatggagg tcttcgagga ggagtatcag
     4981 gagggcatgt ccatggagga cgcgatcatc ctcggcctga aggccttgaa gaaggccacc
     5041 gaggaggaga acctcaaccc caaggccgtg gagatcggcg tggtccgcca cggcgagaac
     5101 ttccatcgtc tggacgagcc cgacgtggaa acctatatcg ccaaggtcaa tcaggactga
     5161 gacggagtat cagatggtag acctggaaga ggcgattgtg gcccgtctgg agtcccatgg
     5221 cgagaccttt gaggtcctca tcgaccccaa ggtagtcaac cacctcaaag agggcaagga
     5281 cgtcgatctg atcgactaca tggtcatcga tgagatattc aagaacgccc gcaaggggac
     5341 cagggcatcc gaggagaagc tgagggaggt cttcggcacg accgatcccg tggagatcgc
     5401 caaggtgatc atcctgaagg gggaggtcca gctgaccgct caacagcgca aggagatgct
     5461 tgagagcaag aggctcagga tcatcgccac catcgcccgc aatgccatca acccccagac
     5521 cggcggcccg cataccccgg cgcgcatcga gcttgcgatg gaggaggcta aggtccacat
     5581 cgatccgttc aagcccgtgg acctccaggt gcagcacgtc ctggagaagc tccgccctct
     5641 catccccatc cgtttcgaca aggtgcgcat agccgtcaag ctcaaggccg acgagtacgg
     5701 ccggtgctat gaggacatga aggagatggg gaagataacc agggaggaat ggcagaagaa
     5761 cggcgattgg atcggcgtgg tcgagatccc tgcgggcctc cgcgacgacc tgttccagcg
     5821 cctgaacgcc aagacccacg ggaacgtgga gaccaagcag ctaaagtgat gtaatataca
     5881 tttccaagag ttgatcaaat gagcaaagag agcacagcca gagaactggt gcttcccggg
     5941 gaccttctgg accctgggac catgaagccc ggcgagggga cgtacgtcca tgacggcaag
     6001 atcttcgctg cgatgctcgg catcaagagc gtcaagtcca atttcgtgaa cgtcattccc
     6061 ttgggcggga ggtacattcc ggcccccggc gatatggtca taggcaaggt gatcgacatc
     6121 gggccctcga actggctggt ggacatcaat tcgccctacc ctgccccgct gcacgtcaat
     6181 gaggtgccat ggagggtcga gttcggggac accgcccgct acctgagtgt gggggacacc
     6241 ttgctggcga aggtgctctc ggtggatgag acgaagaggg tgcaggtgac catgaaggag
     6301 caggggctgc gcaagctgca gggagggcag atcctggaga tctcccacag caaggtgccg
     6361 agggtgatag gcaagaacgg ctccatgatc cagatgatca agaactacac caactgccgc
     6421 atattcatcg gccagaacgg caggatctgg gtagatggga acatggacga catggtgcac
     6481 gccatgcgtg ccatcaagat gatcgaagag ggcgcacaga cgctgaagct gaccgagcgg
     6541 gtgaaggagt tcctggactc ggtctacagg aaggaagagt gaggtgttct aaatgggtgg
     6601 aaattccgat atgaagctca tcgatgagaa tggcataagg atcgatggcc ggaaggtgga
     6661 tgagctccgg ccgataagga tcgaggcggg tgtgctcaag agcgccgacg gttccgcgta
     6721 cgtcgagata gggaagaaca aggtcctggc cgccgtgtat ggccccaggg agtgccatcc
     6781 ccggcatatg cagaaccccg ccaaggcgat cgtccaatgc aagtacaata tgatctcctt
     6841 ctcggtctcc gaccgcaaaa ggcc
//
LOCUS       DAOSYN010000058         6643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_9801_length_6643_cov_3.638737, whole genome shotgun sequence.
ACCESSION   DAOSYN010000058 DAOSYN010000000
VERSION     DAOSYN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9801_length_6643_cov_3.638737"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PLJ11_03650"
     CDS             complement(<1..1242)
                     /locus_tag="PLJ11_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03795.1"
                     /translation="MKKIVSALFSSLLFALLLCSLAVPAFASAPMAEAPEWNEGDRWG
                     YGVEVDFGEDLGDLMANLTDLLEMVDEVQSVNELSADGGSEFWMLFEVTDVTEDSYVM
                     DMDLASMVTLDAALSVTASMPVAGTYHLGDEIETENMTISLEAGAEVTLVVNVEITYD
                     KETMAIEAVSLSAKLLGTADFTGENIPHVDRGFTNTTYRYEDYDVHAELKADLDLDLT
                     FDPCLNIWDFPMSVNDTWWSNFTASLSGNLTGYLDVQGLPDDVEDEIFNDEFVEETGI
                     TGFPIVFEDIEGDEGSPIDKGVIGPFDSGEIDLWFNCTDYFEDLYDMTNVYEIRVMTG
                     GEGVPFAFYYSSDAGFMAAFKMDAGELNETVGGMIDEDMTTMASSDPDVVEENINEIV
                     ERQGELEEDDGILGFFTDPPYL"
     gene            complement(1422..3101)
                     /gene="thsB"
                     /locus_tag="PLJ11_03655"
     CDS             complement(1422..3101)
                     /gene="thsB"
                     /locus_tag="PLJ11_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HOO03796.1"
                     /translation="MAYGMGQGQQPIIVLREGTERSKDKEAQYNNIAAARAVADAVKS
                     TLGPKGMDKMLVDSMGDVVITNDGVTILKEIDVQHPAAKMVVEIAKTQDTECGDGTTS
                     SVIIAGELLKKAEALIEQNIHPTIVANGYKMAAAEAIRILDTIAVPITSDDTEKLKRV
                     AMTAMTGKSVGGQGEFLAELAVKAVKAVAERTGNKYTVDVDNIKVEKRTGGSIAETEI
                     IEGIVIDKERVHPRMATQVKKAKIALLSVPMEVKKTEVDAKIQIRDPSQMQRFLDEEE
                     AVLKGMVDQVAASGANVVFCQKGIDDLVQHYLAKAGIFAARRLKESDMEKIAKATGGT
                     IVGKIDNLTKNDLGVAELVEQRKVGENDMIFITGCKNPKAISIVVRGGTEHVADEVER
                     SLHDALRVIGVALEDGKIVPGAGAPEIELSLRLADYASAVGGREQLAIEAFASAMEII
                     PWALAENAGLDAIDMVIQLKSAHEKKAANAKNMGLDLDAGKPADMLKANIIEPLRVKT
                     QAVKSAAEVANMILRIDDVIASKRAPPAPPGGNDGMGGMGGMGGMGGMGGM"
     gene            3263..3553
                     /locus_tag="PLJ11_03660"
     CDS             3263..3553
                     /locus_tag="PLJ11_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03797.1"
                     /translation="MDEDLERFRVDLGYCKACLGMQGGREQRIYRFPNGYGASLISAP
                     RLGGPPRWTVMALRFDGDEYEAVEVPEVTNGSTGDWSASVSALRKLMARPHF"
     gene            3604..4890
                     /locus_tag="PLJ11_03665"
     CDS             3604..4890
                     /locus_tag="PLJ11_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nre family DNA repair protein"
                     /protein_id="HOO03798.1"
                     /translation="MQGFEPLPLGQEAFAASRREGSSLCHVCKGSKNLCGKDRCPLMI
                     KFYSYSKTKRLIDAQDLHGSSPPGVFVGRYGYPKVDIGPLVPPDLGDTVIMDTPEQWM
                     GKTMEEIVDFRFRLVRGKHLVNVRDFQSAGRIVDLTRELALSVNSVDVEAKFSKRPSG
                     RIVLDDNIQPFGPSARLERLRIGNPKYDHKVEKAYYDTDLRAVDAVKELYRNDVLISR
                     IEKAFSVGAFGREGARRLVPTRWSITAVDDMLGKDLLRTTKTFPLINEFRVYDWDQLD
                     NRWSVLLTPTSWRYELIEAWYPNTVWNPAGLQVEMISSHEFFDGRKEYAEIGGCYYAA
                     RLAVNELLQRERRQAGAVIFREAHPGYLLPVGVWNVRENVRMALTQRPRRFDTLDQAL
                     AHVQTRMDIPISTWTAHSAVLRDLRHQRRIDDYVDP"
     gene            4877..5713
                     /locus_tag="PLJ11_03670"
     CDS             4877..5713
                     /locus_tag="PLJ11_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03799.1"
                     /translation="MSTLDLFLEMEDGRAAPSVPRVREVRTRVALPASRLPGLDYSLN
                     PYVGCQHDCLYCYAPYVTKRPRGKWTEVLARTDLPHVLSKELRGKRGTIGLGTVTDPY
                     QEAERHLLLTRRCLIEIIPHGLAVSALTKSDLVLRDIDLYKELRGEVGITVTSVSPEL
                     SRAMEPGAPLPARRLDALRGLTEEGVNAYALIGPLLPMLEERDVIEMVDALAATGIER
                     AMLDRFRPRPGMFDDIRARPACKGLGDGLERAHRERDYSALEALVRRECSRKGLRCVD
                     AF"
     gene            complement(5964..6338)
                     /locus_tag="PLJ11_03675"
     CDS             complement(5964..6338)
                     /locus_tag="PLJ11_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOO03800.1"
                     /translation="MVENEEERVRKVLERTREEYGFVPLVSEVLSERPDLYLPYAELS
                     SRLFFKPQHMDRRTAELAAIAAGAALGSENCLNVHIIQAFRLGVTEEAVMEALMLGAF
                     MSMNRSCSVALRCLRSATEDRV"
BASE COUNT         1184 a         2073 c         2025 g         1361 t
ORIGIN      
        1 gagatagggc gggtcggtga agaaccctag gatcccgtca tcctcctcga gttcgccctg
       61 gcgctccact atctcgttga tgttctcctc gactacgtcc gggtcggagg atgccatggt
      121 ggtcatgtcc tcgtcgatca tcccaccgac ggtctcattc agctctcccg cgtccatttt
      181 gaacgcggcc atgaagccgg cgtcagagga gtagtagaag gcgaacggta cgccttcccc
      241 gcccgtcatc accctgatct catagacatt ggtcatgtca tagaggtcct cgaagtagtc
      301 ggtgcagttg aaccacaggt cgatctcgcc ggagtcgaac ggcccgatga cccccttgtc
      361 gatcggggag ccctcatcgc cttcgatatc ctcgaacacg atcgggaagc ccgttatccc
      421 ggtctcctcg acgaactcgt cattgaagat ctcgtcctcc acatcatcgg gcagcccctg
      481 cacgtccagg tagccggtca ggttgccgct cagggaggcg gtgaagttcg accaccaggt
      541 gtcgttgacg ctcatcggga agtcccagat gttcaggcag gggtcgaagg tgaggtccag
      601 gtccagatcg gccttcagct ccgcgtgcac atcgtagtcc tcgtaccggt aggtcgtatt
      661 cgtgaaccca cggtccacat gagggatgtt ctctcccgta aagtccgcgg tgcccaggag
      721 cttggcgctg agggaaacgg cctcgatggc catagtctcc ttgtcatagg tgatctccac
      781 attgaccacc agggtcacct ccgctccagc ctcgagggag atcgtcatgt tctcggtctc
      841 gatctcgtcc cctaggtggt atgtgcccgc caccggcatg ctggcggtca cggacagggc
      901 ggcatccaat gtaaccatgg aggccaggtc catgtccatg acgtagctgt cttcggtcac
      961 atctgtgacc tcgaacagca tccagaactc ggacccgcca tccgcgctca gctcattgac
     1021 cgattgtact tcatccacca tctccaagag gtcggtcaga ttggccataa ggtcgcccag
     1081 gtcctctccg aagtccacct cgaccccgta cccccatctg tccccctcgt tccactctgg
     1141 tgcctcagcc atcggagcgg aggcgaacgc ggggaccgcc agggagcaca ggaggagcgc
     1201 gaacagcaga gagctgaaca atgcgctaac tatcttcttc atgcttcatc ccttcttaac
     1261 ggtcggttgg taagcgattt gttttgaatt gaacatttca ctattaatat cattgcgctt
     1321 cgaattatca aaacgagaaa tcaaaagagc gagaaatcaa aagaggaata ggaaaatggt
     1381 ttaaagggac cttacgcggg aggtttggaa gggacgcttg cttacatccc gcccattcca
     1441 cccatcccgc ccatgccacc cattccgccc atgccgtcat tgccgcccgg gggtgccggc
     1501 ggggccctct tggaggcgat gacatcgtcg atcctcagga tcatgttggc gacctcggct
     1561 gcggacttca ccgcctgggt cttgaccctc aggggctcga tgatattcgc cttgagcatg
     1621 tccgccggct tgccggcgtc caggtccagg cccatgttct tggcgttggc ggccttcttc
     1681 tcatgagcgg acttcagctg gattaccatg tcgatggcgt ccaggcctgc attctcggca
     1741 agggcccagg ggatgatctc catggcggag gcgaacgcct cgatggccag ttgctcgcgg
     1801 cccccgacag cggaggcgta atcggccagt cgcagggaca gctcgatctc cggggcgcca
     1861 gcaccgggaa cgatctttcc atcctccagg gcgacgccga ttacgcgcag ggcgtcgtgc
     1921 agggacctct cgacctcatc ggcgacgtgc tcggtgcccc cgcgcacgac tatgctgatg
     1981 gccttcgggt tcttgcagcc ggtgatgaat atcatgtcgt tctccccgac cttcctctgc
     2041 tcgaccagct cggccacccc gaggtcgttc ttggtcaggt tgtctatctt gccgacgatg
     2101 gtgcctccgg tggctttggc gatcttctcc atatcggact ccttcaacct tcgggcggcg
     2161 aatatcccgg ccttggcgag gtagtgctgc accaggtcgt cgatgccctt ctggcagaat
     2221 acgacgttcg cgcccgaggc tgccacctgg tcgaccattc ccttcaggac ggcctcttcc
     2281 tcgtccagga agcgctgcat ctgggacggg tcccttatct ggatcttggc gtccacctcg
     2341 gtcttcttga cctccatcgg cacgctcagc agggcgatct tcgccttctt gacctgggtg
     2401 gccattcggg ggtgcacgcg ttccttgtcg atcacgatgc cctcaattat ctcggtctcg
     2461 gcgatggagc cgccggtcct cttctcgacc ttgatattgt ccacatcgac cgtatacttg
     2521 ttcccggtcc tctcagcgac ggccttcacg gccttcacgg ccagctcggc aaggaactcc
     2581 ccctgcccgc ccacgctctt gccggtcatg gcggtcatcg ccacgcgctt cagcttttcg
     2641 gtgtcatcgg aggtgatagg cacggcgatt gtgtccaaga tcctgatggc ctcggctgcg
     2701 gccatcttgt acccgttggc tactatcgtc gggtggatgt tctgctctat cagcgcctcc
     2761 gccttcttca acagctcccc cgcgatgatc acggaggagg tggtcccatc cccgcactcg
     2821 gtgtcctggg tcttggcgat ctccacgacc atcttggcgg ccgggtgctg aacgtcgatc
     2881 tccttcagga tcgtcacgcc atcgttggtg atgaccacat cccccatgga atcgaccagc
     2941 attttgtcca ttcccttggg gcccagggtc gacttgaccg cgtcagcgac cgcacgggcg
     3001 gcggcgatgt tgttgtactg tgcttccttg tcctttgatc tctctgtacc ctcacgaaga
     3061 actatgatag gctgttgtcc ttgacccatt ccgtaagcca tagtgagcct ccttacggag
     3121 ggtattttat cgcttctata tatagatatc taattcagcg ttcctaaaaa agagctgaga
     3181 cgcggttggg aacgcctacg ccatataaaa gattagccat ccgcgcccca atattttttc
     3241 gttcagtgac cgatttcagt tcatggacga ggaccttgag aggttccggg tggaccttgg
     3301 gtactgcaag gcctgcctgg ggatgcaggg aggaagggag cagcggatct acaggttccc
     3361 caatggctat ggggcgtccc tcatctccgc cccccgcctg ggtggaccac ccaggtggac
     3421 ggtgatggcc cttcggttcg acggggacga gtatgaggcc gtggaggtgc cagaggtgac
     3481 gaacggcagc actggggatt ggagcgcctc tgtctcggcc ttgaggaagc tcatggcaag
     3541 gcctcatttt taaggccccc tggaaaacgg tcaaatacgg ttgtcgcggt agaacgggac
     3601 gagatgcagg gcttcgaacc gctgccactg ggccaagagg cgttcgccgc atcgaggagg
     3661 gaggggtcca gcctatgcca tgtatgcaag ggctccaaga acctctgcgg caaggacaga
     3721 tgccccctca tgatcaagtt ctattcctat tccaagacga aacgcctcat cgacgcccag
     3781 gacctccatg gcagctcccc gccgggggtc ttcgtcggcc gctatggcta ccccaaggtg
     3841 gacatcggcc ccctggtgcc tccggacctg ggggacacgg tcatcatgga caccccggag
     3901 caatggatgg ggaagaccat ggaggagatc gtggacttcc gcttccggct tgtgcgggga
     3961 aagcaccttg tgaacgtgcg ggacttccag agcgccgggc gcatcgtgga cctcacgagg
     4021 gaattggccc tctcggtcaa ttccgtggac gtggaggcca agttctccaa gaggccctcc
     4081 ggccggatag tcctggacga taacatccag ccattcgggc cctccgcaag gctggagagg
     4141 ctgcgcatcg gcaaccccaa gtacgaccac aaggtggaga aggcctatta cgacacggac
     4201 ctgagggccg tcgacgcggt caaggagctt tacaggaacg atgtcctcat ctcccgcatc
     4261 gagaaggctt tcagcgtggg ggccttcggc cgggagggcg ccaggcgcct tgtgccgacg
     4321 cggtggagca tcaccgctgt ggacgacatg ctggggaagg acctcctccg gacgacgaag
     4381 acctttccgc tgatcaacga gttcagggtg tatgactggg accagctgga caaccgctgg
     4441 agcgtgctgc tcacccccac ctcctggcgg tacgagctga tcgaggcctg gtatcccaac
     4501 accgtctgga acccggccgg gctgcaggtg gagatgatct cctcgcacga gttcttcgac
     4561 ggccgcaagg agtacgcgga gataggggga tgctactacg cggcaaggct ggcggtgaac
     4621 gagctgctgc aacgggagcg tcgtcaggcg ggcgcggtca tcttcaggga ggcgcacccc
     4681 ggctaccttc tccccgttgg ggtctggaac gtcagggaga acgtccgcat ggcgctcacc
     4741 cagaggcccc gcaggttcga caccctggac caggcgctgg cgcatgtgca gacccgtatg
     4801 gacatcccta tatcgacctg gacggcgcac agcgctgtgc tcagggacct gaggcaccag
     4861 aggaggatag acgactatgt cgacccttga cctctttctg gagatggagg acggccgggc
     4921 tgcccctagc gtcccgcgcg tccgggaggt acgcacgcgg gtggcgctcc cggcgtcccg
     4981 cctccccggc ctcgactact ccctgaaccc ctacgtggga tgccagcacg actgcctgta
     5041 ttgctacgcc ccctatgtca ccaagaggcc gcgcgggaag tggacggagg tcctggcccg
     5101 gacggacctt ccgcatgtgc tcagtaaaga attgaggggg aagaggggga ccatagggct
     5161 cgggaccgtc accgacccct atcaagaggc ggaaaggcat ctgctgctca cccggcgctg
     5221 ccttatcgag atcatccctc atgggctggc ggtcagcgcc cttacgaaat cggacctcgt
     5281 cctgagggac atagacctct acaaggagct caggggggag gtgggcatca ccgtcacgtc
     5341 ggtgagccct gagctgagca gggcgatgga gccgggggcc cccctccccg cgaggcggct
     5401 ggatgccctt cgtggattga ccgaggaagg ggtgaacgcg tacgccctca tcggcccgct
     5461 cctcccgatg ctggaggaga gggacgttat agagatggtc gatgccctcg cggccacggg
     5521 catcgaacgg gcgatgctgg accgcttccg gccgcggccg gggatgttcg atgacatccg
     5581 ggcgcggcca gcgtgcaagg gccttgggga tgggctcgag agggcccacc gggaaaggga
     5641 ctattcggcg ctggaggccc ttgtgcggag ggagtgctcg cggaaggggc tccggtgcgt
     5701 ggacgccttc tgatcgctgc ccgccctatt agcctccagg gtttcctcga gggcgggggg
     5761 cgagccgttc attcctttac agcgggaaag gaggcacgcc cctggcctcg gctatcttcc
     5821 gttccaatcc tcaattccga tcctctggtg atcacggtcc cagttcttgg gtgggggcgt
     5881 ggtcctggac cgtccttcta tgagcacacg ttgaggatcg atcccgtagg tgaatgactg
     5941 cgcccgcctc cgcggccgag gcctcataca cggtcctccg tcgccgatct caggcagcgg
     6001 agggcgacgg agcagctgcg gttcatgctc atgaaggcgc ctaacatgag tgcctccatc
     6061 accgcctcct cggtcacccc gagcctgaag gcttggatga tgtgcacgtt caggcagttc
     6121 tcgctgccca gggccgcccc cgcggctatc gccgccagct cggccgtcct ccggtccatg
     6181 tgctggggct tgaagaagag cctgctcgac agctccgcgt aggggagata caggtcgggc
     6241 ctttcggaca ggacctccga caccaatggc acgaacccat actcctccct ggtcctctcc
     6301 aggaccttgc gcaccctctc ctcctcgttc tcgaccatgg aaggggcatg gcggtggacg
     6361 gtaattgagc tatcgtcccg agcgcggggc aatcgttatg agcgagaggt tcgttccctc
     6421 aatggtatgg gaacggtcac ttgtccgagc tgtggaacgg ccaatgatga cggtgctgtg
     6481 ttctgtcagc gatgcggggg gcgcatggac gccccgcctg ggcccgtgaa ggtccctcca
     6541 gccaattgga accagacccc cttcgaccgc gccttccggg gcgggggacc gctgctcaag
     6601 gcccttctca gcgtgatctt cgtccttctt gtcatggagg tgt
//
LOCUS       DAOSYN010000059         6611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_9859_length_6611_cov_4.175717, whole genome shotgun sequence.
ACCESSION   DAOSYN010000059 DAOSYN010000000
VERSION     DAOSYN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9859_length_6611_cov_4.175717"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLJ11_03680"
     CDS             <1..367
                     /locus_tag="PLJ11_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO03801.1"
                     /translation="APGDSGVPGVFLCGSALSGHRDGSETQAARAAAVSLARELRSPA
                     PRTPKARVDRERCSKCLTCLRLCPYGAPSLEHGEVVVSVERCQGCGLCVAMCPGMAIE
                     MPPSDLRAEVGATRMGGMG"
     gene            364..738
                     /locus_tag="PLJ11_03685"
     CDS             364..738
                     /locus_tag="PLJ11_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOO03802.1"
                     /translation="MIVCYCCARSGMPAALRAMEDGGLDDVLLREVPCTGAVESWEVL
                     RSFRQGADGVLLVGCLLENCAHHYGSEVASRRAARLSQVMSDIGSVPGKVAMVHLAEH
                     QADRFRRAVEGVRKANGQEEGR"
     gene            735..1427
                     /locus_tag="PLJ11_03690"
     CDS             735..1427
                     /locus_tag="PLJ11_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOO03803.1"
                     /translation="MIIAERKPLPEVLEMLEGAKDVLVLGCRACTAICLAGGEREAVE
                     MREALSLHSELKGKGWNVSALTVERACEREFLSPIEGHTGTVVSMACGVGAQVVQGLF
                     PSLRVVPGVNTSNMGAPEGMGVFVERCAGCGDCIIHLTAGICPIARCAKGLLNGPCGG
                     SKGGRCEVSPETPCAWEQIVRGLEAQGRTDLLEAIIPPRDWRPSRSGGPRRIVNAEAA
                     PGPAQREWRGED"
     gene            1424..2323
                     /locus_tag="PLJ11_03695"
     CDS             1424..2323
                     /locus_tag="PLJ11_03695"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOO03804.1"
                     /translation="MSVSRLQGTLDRGAIAVTAEIGPPRSCEVERLRAMARALKGSAH
                     AFNLTDNQAANVRMSSIASSVICLQEGLEPVMQMTCRDRNRIAMQSDVIGASALGIRN
                     VLCISGDHQTFGNHPQAKNVYDLDSVQELMVLRRLREGANWAGETLAEPPQLFLGAAA
                     NPFADPFEHRVTRLAKKVEAGAQFVQTQCVLDLDRFRRFMALVVERGLHERVHIIAGA
                     IPLRSHKAALFMRDKVAGMSVPDEVIDRLRRAADPKEEGIRLCVETMQELVSIEGVHG
                     VHITAIAWEDVVPRLVREAGLTP"
     gene            complement(2343..2873)
                     /locus_tag="PLJ11_03700"
     CDS             complement(2343..2873)
                     /locus_tag="PLJ11_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03805.1"
                     /translation="MSGELGRIDVPVLRWEQPKWTQRFHRLIGGETTYATLEWPQAFG
                     STAVSSSREGRYTFKRGGFLRPFVTVRRQGSEVDLGRLGLDWKGNGELVLNNVQRFGF
                     VRQSIMGFDYEVTGKDGTRLFSSRKEWAPIKHGGEVHITPAGMADRDIGMLITLCWYV
                     AVMASEDVMVGTAAGA"
     gene            2956..4077
                     /locus_tag="PLJ11_03705"
     CDS             2956..4077
                     /locus_tag="PLJ11_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO03806.1"
                     /translation="MNDVAGQFKPIDDEWHAEARKRFASPFMGTNYGHIPVDPMMLSH
                     AAVANGHTIREFYESPELGAHCVAVAMERYDLLPVTHWYYSLPWLAELGMEVQYMDWM
                     PPVPKAPIILDPSQVEELQVPGREELLRGATLDQLFRSNDYVKRHLPKMFSPLAIATD
                     LTGSGAQLVGVENFIMWTLTEPDLAHALVRIYMETAAEGAGLIAERYGEAVITTGSVL
                     GNNDIFSDAMVRDFSGRYLNEFVMKCFEKGAGPQVFYHLCGNHETDYKVLKEAIVWSP
                     MTILHIGYKGREVFPSDLLVQEFGGYATCMGSVDTKLMLHGTPEKVYEQAKLQVLRGR
                     DCSSGYILGTACEVPPFTPPENIRALNRAAEDFGTYGTW"
     gene            complement(4280..5056)
                     /locus_tag="PLJ11_03710"
     CDS             complement(4280..5056)
                     /locus_tag="PLJ11_03710"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOO03807.1"
                     /translation="MLVISERINGLYAAVGRAIDARDEGFIQRHAVLQMECGAQALDL
                     NVGPGRDDGPAAMEWLVRTVQEVSDLPLCIDTPGIKTMTAGVKACRNRLIINSTTAEA
                     RKMQALFPLAKEHDADVICLTMDERGVPNDAESRAEMAMLMITTAMEHEIMPDRLYLD
                     PLVLPIKAAQDQPMKVIKAMQLFQTLNDPAPRTVVGLSNVSNGAKERGILNRTFLAML
                     MGAGLSAAIVDVQDAELMRLVKAGELLRAEKLYCDDFLRA"
     gene            5258..>6611
                     /gene="purH"
                     /locus_tag="PLJ11_03715"
     CDS             5258..>6611
                     /gene="purH"
                     /locus_tag="PLJ11_03715"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOO03808.1"
                     /translation="MVRIERAVISVSDKEGIVDFCKGLSQMGVELLSTGGTAKLLSSH
                     GLKVVNISDYTGSPEMMDGRVKTLHPAIFAGLLARRDSEEHMRQLREAGLKRIDMVVV
                     NLYPFREAVLDKGGDLNEVIENIDIGGPSMIRAAAKNSASVAVVTDPKRYPALLDELR
                     ANGGELSQATLRSLMLEAFRLTAYYDALIASHLGSTFGDEGFPGTYCMGLEKRQDLRY
                     GENPQQAAAFYVDPFAKGVCVSRSEQLHGKELSYNNILDLEAALELVREFERPTAIVI
                     KHTNPCGVASADTISEALVQAYNVDPLAAFGCVIGLNRPVDVPTATEVSQHFVDCLIA
                     PTFEPAALEVLRRKKNIRLLRTNAPIALDEAPEWRMKRIKGGLLVQTNRYVPITAKDL
                     KVVTERAPTEEEVRGLLFANKVCKHVWSNSIILAKGETVVGIGAGQMSRVDASMIAVH
                     K"
BASE COUNT         1143 a         2168 c         2229 g         1071 t
ORIGIN      
        1 cgctccgggg gacagcgggg tcccgggggt cttcctctgc ggctccgccc tctctgggca
       61 tcgcgacggc tcggagacgc aggcggccag ggccgcggcg gtctcgttgg ccagggagct
      121 gcggtccccc gcgcccagaa cgccgaaggc ccgggtggac cgcgaacggt gctcgaagtg
      181 cctgacctgc ctccggctgt gcccttacgg cgccccgtcc ctggagcacg gggaggtggt
      241 cgtatccgtc gaacgctgcc agggctgcgg actgtgcgtg gccatgtgcc cgggcatggc
      301 catcgagatg cccccctccg acctgagggc ggaggtcggg gccaccagga tgggagggat
      361 gggatgatag tctgctactg ttgcgcaagg tccgggatgc cggcggccct cagggccatg
      421 gaggacgggg gcttggacga cgtgctcctg agggaggtcc cctgcacggg ggcggtggag
      481 tcgtgggagg tcctgcggag cttccggcag ggggcggacg gtgtgctcct cgtcggctgc
      541 ctcctggaga actgcgccca tcactacggc agcgaggtgg cctcgaggcg ggccgcccgc
      601 ctctcccagg tcatgagcga catcggctcc gtccccggca aggtggccat ggtgcacctg
      661 gccgaacatc aggcggaccg cttccggagg gcggtggaag gggtccggaa ggccaacggc
      721 caggaggagg gacgatgatc atcgccgaga ggaagccgct gcccgaggtg cttgaaatgc
      781 tggaaggggc gaaggacgtc ctggtcctgg gctgcagggc ctgcaccgcc atctgccttg
      841 ctggagggga gagggaggcc gtggagatgc gagaggcgct ctccctgcac tccgaactca
      901 aggggaaggg gtggaatgtc agcgccctca ccgtggagag ggcgtgcgag cgggagttcc
      961 tctctcccat cgaaggccac acgggcaccg tcgtcagcat ggcctgcggg gtgggggccc
     1021 aggtggtcca ggggctgttc ccctcgctcc gggtcgtccc cggggtgaac acgtccaaca
     1081 tgggggcgcc cgaggggatg ggcgtgttcg tggagaggtg cgcgggctgc ggcgattgca
     1141 tcatccatct cacggccggc atctgtccta ttgcccgctg cgcgaagggc cttctgaacg
     1201 ggccgtgcgg ggggagcaag ggcggaaggt gcgaggtgtc cccggagacc ccctgcgcct
     1261 gggagcagat cgtcaggggg ctcgaggcgc agggccggac ggacctgctg gaggccatca
     1321 tcccccccag ggactggcgc cccagccgga gcgggggccc caggcgcatc gtcaacgcgg
     1381 aggcggcgcc gggcccggcc caaagggaat ggaggggaga ggattgagcg tctcccggct
     1441 gcaggggacg ctggacaggg gggccatcgc ggtcacggcg gagatcgggc cgccgcgctc
     1501 ctgcgaggtg gagaggctga gggccatggc ccgggcgctc aagggaagcg cgcacgcctt
     1561 caatctcacg gacaaccagg cggcgaatgt gcgcatgtcc agcatcgcct cgtcggtcat
     1621 ctgcctgcag gaggggctgg agccggtgat gcagatgacg tgtcgggacc gcaaccgcat
     1681 cgcgatgcag agcgatgtca tcggcgcaag cgccttgggc ataaggaacg tgctgtgcat
     1741 aagcggcgac caccagacct tcgggaacca cccccaggcc aagaatgtgt acgacctgga
     1801 ctcggtccag gagctcatgg tcctcaggcg cctccgggag ggggcgaact gggccgggga
     1861 gaccctcgcc gaaccgccgc agctcttcct gggagcggcg gccaacccct tcgccgaccc
     1921 gttcgagcac cgggtgacaa ggctggccaa gaaggtcgag gccggggccc agttcgtcca
     1981 gacccagtgc gtcctggacc tcgaccggtt ccggaggttc atggcgctcg tggtggagag
     2041 ggggctgcac gagcgggtgc acatcatcgc cggggcgatc ccgctgcgct cgcacaaggc
     2101 cgccctcttc atgagggaca aggtcgccgg gatgagcgtc cccgacgagg tcatcgaccg
     2161 ccttcggcgg gcggccgacc ccaaggagga ggggatcagg ctctgcgtgg agacgatgca
     2221 ggaactcgtc tcgatcgagg gcgtgcacgg cgtccatatc accgccatcg cctgggagga
     2281 tgtggtcccc cggctcgtgc gggaggccgg gctcaccccc tgagcccctt ccaattagaa
     2341 cgtcaggcgc ccgcggcggt gccgaccatg acgtcctccg aggccatcac cgccacgtac
     2401 cagcacaggg tgatgagcat gccgatgtcc cgatcggcca ttcccgccgg ggtgatgtgc
     2461 acctccccgc catgcttgat gggggcccac tccttccgcg aggagaagag ccgggtgccg
     2521 tccttgccgg tcacctcata atcgaacccc atgatgctct gccggacgaa gccgaaccgc
     2581 tggacgttat tgaggaccag ctcgccattg cccttccagt ccaggccgag cctcccgagg
     2641 tccacctcgg acccctggcg cctgaccgtc acgaagggcc gcaggaaccc tcctcgtttg
     2701 aaggtgtacc tcccctcccg ggaggacgat acggcggtgg aaccgaaggc ctgcggccac
     2761 tccagcgtgg cgtaggtcgt ctcccctcct atcagccgat gaaagcgttg ggtccacttc
     2821 ggttgctccc atctcagcac cggcacgtct atgcgcccca gctctccgct catggtcata
     2881 atattctccg atgcctttat atgtcctccc cctcaccgca tctgacaaat actaccagcc
     2941 ccccttccaa ggaccatgaa cgacgtggca gggcagttca agccgataga cgacgaatgg
     3001 cacgccgagg ccaggaagcg cttcgcgtcc cctttcatgg gcacgaacta cggccacatc
     3061 ccagtggacc cgatgatgct ctcccacgcc gcggtggcca acggccacac catccgggag
     3121 ttctacgaga gccctgagct gggagcgcac tgcgtggccg tcgccatgga gaggtacgac
     3181 ctgcttcccg tcacccactg gtactactcc cttccctggc tcgccgagct gggcatggag
     3241 gtccagtaca tggactggat gcccccggtg cccaaggccc ccatcatcct cgacccctcc
     3301 caggttgagg agctccaggt cccgggcagg gaggagcttc tcagaggcgc gacattggac
     3361 cagctgttcc gctccaacga ttacgtcaag aggcacctgc ccaagatgtt ctccccgctc
     3421 gcgatcgcca ccgacctgac cgggagcggg gcccagctgg tcggcgtgga gaacttcatc
     3481 atgtggacgc tgaccgagcc tgacctggcc catgccctgg tccggatcta catggagacc
     3541 gcggcggagg gcgcgggcct catcgccgag cggtacggcg aagcggtcat caccaccggc
     3601 tcggtcctgg gcaacaacga catcttctcc gatgcaatgg tccgggactt ctcgggaagg
     3661 tacctcaacg agttcgtgat gaaatgcttc gagaagggag cgggccccca ggtgttctac
     3721 cacctgtgcg ggaaccatga gacggactac aaggtcctga aggaggcgat cgtctggtcc
     3781 ccgatgacga tccttcacat aggctacaag ggcagggagg tgttcccgtc cgacctcctg
     3841 gtccaggagt tcggggggta cgccacctgc atgggctcgg tcgacaccaa gctcatgctg
     3901 cacggcaccc cggaaaaggt ctacgaacag gccaagctcc aggtcctccg ggggcgcgac
     3961 tgctcctccg ggtacatact gggaacggcg tgcgaggtgc cgccgttcac cccgccagag
     4021 aacatccgcg ccctgaacag ggccgcagag gacttcggga cctacgggac ctggtgaggg
     4081 ctctcggcca tccctggagc ccttcggcca tggaggaaaa gatcgtgaag ggactggcca
     4141 tcaccagctg gatgggatga gcatagggac cggcagcgtc ccccccgggc ttgaggtagg
     4201 ggaatgcatg gggatcgagc cccaccagcc agggtccggg cgcgccccgt cctcctgtca
     4261 aggcgatggc gtcgccatgt cacgccctga ggaaatcatc gcagtagagc ttctccgccc
     4321 tcagcagctc ccccgccttg acaaggcgca tgagttcagc gtcctgcacg tccacgatcg
     4381 cagcgctgag ccccgccccc atcagcatcg ccaggaacgt cctgttgagg atgccccgct
     4441 ccttggcccc gttggagaca ttggagaggc cgaccacggt gcggggggcg ggatcgttca
     4501 gggtctggaa cagctgcatg gccttgatga ccttcatcgg ctggtcctgg gcggccttga
     4561 tcggcaggac cagggggtct aggtaaaggc gatcgggcat tatctcatgc tccatcgccg
     4621 tggtgatcat gagcatggcc atctccgccc tcgattccgc gtcgttcggc acgccacgct
     4681 cgtccatggt caggcagatc acgtccgcat cgtgctcctt tgccaggggg aagagcgcct
     4741 gcatcttcct cgcctcggcc gtagtggaat tgatgatcag cctgttgcgg caggccttga
     4801 cgccggcggt catggtcttt atgccaggcg tgtcgatgca cagggggagg tcggaaacct
     4861 cctgaacggt ccgcacgagc cattccatgg ccgccgggcc gtcgtccctc cccgggccga
     4921 cgttcaggtc cagggcctgg gccccgcact ccatctggag gaccgcatgc cgctggatga
     4981 acccctcgtc ccttgcgtcg atggcccgtc cgaccgcggc gtacaggccg tttatccgct
     5041 cgctgatgac caacatctcg gttcggtagg ctatccccgc gcaccgcttt aagcattccc
     5101 gccggggtcc tcagggatgt gacaccgagg ggtgcgcttc gaattggcca gccaaagggt
     5161 gattccgata ggccatcgct atctttatta cggaacgggc gatatatccc tggcaaacca
     5221 tccttggtgg tctgcctttc ccaccgaagg aatgcacatg gtcaggatcg agagggcggt
     5281 catcagcgtc tcggacaagg agggcatagt cgacttctgc aaaggactga gccagatggg
     5341 cgtggagctg ctgtccacgg ggggcacggc caagctcctc tcctcccacg gcctcaaggt
     5401 ggtcaacatc tctgactata ccggttcgcc agagatgatg gacggcaggg tgaagacgct
     5461 ccaccccgcc atattcgccg ggctgcttgc ccgcagggac tccgaggagc acatgcgcca
     5521 gctcagggag gcgggcctca agcgcatcga catggtggtc gtgaacctct atcccttccg
     5581 ggaggcggtc ctcgacaagg ggggcgatct gaacgaggtc atcgagaaca tcgacatcgg
     5641 ggggccgtcc atgatcaggg cagcggccaa gaactcggcc tcggtggcgg tggtcaccga
     5701 cccgaagcgc taccccgccc tgctggatga gctcagggcc aacggcgggg agctgtcgca
     5761 ggccaccctg cgttccctca tgctggaggc cttccgcctg accgcctatt acgacgcgct
     5821 catagcctcg cacctcggct cgaccttcgg ggacgagggc ttcccaggga cctactgcat
     5881 ggggctggag aagcgccagg acctgaggta cggggagaac ccccagcagg ccgcggcctt
     5941 ctacgtcgac cccttcgcca agggggtctg cgtctcccgc tccgagcagc tccacgggaa
     6001 ggaactctcc tacaacaaca tcctggacct ggaggcggcg ctcgagctgg tgagggagtt
     6061 cgagaggccc acggccatcg tgatcaagca caccaacccc tgcggcgtgg cctccgcgga
     6121 caccatctcc gaggcgctcg tccaggcgta caacgtcgac ccgttggccg ccttcggctg
     6181 cgtcatcggc ctgaacaggc ctgtggacgt gcccacggcc accgaggtct cgcagcactt
     6241 cgtggactgc ctcatcgcgc ccaccttcga gccggccgcc ctggaggtgc tgaggaggaa
     6301 gaagaatatc cgcctcctcc gcaccaacgc ccccatcgcc ctggacgagg cgccggaatg
     6361 gcggatgaag cgcatcaagg gcgggctgct cgtccagacg aacaggtacg tgcccataac
     6421 tgcgaaggac ctcaaggtcg tcaccgagcg ggcgccgacc gaggaggagg tccgcgggct
     6481 gctgttcgcc aacaaggtct gcaagcacgt gtggtccaac agcatcatcc tggccaaggg
     6541 cgagaccgtc gtgggcatcg gcgccgggca gatgtcccga gtggatgcct ccatgatcgc
     6601 cgtgcacaag g
//
LOCUS       DAOSYN010000060         6503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10071_length_6503_cov_4.063586, whole genome shotgun sequence.
ACCESSION   DAOSYN010000060 DAOSYN010000000
VERSION     DAOSYN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10071_length_6503_cov_4.063586"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1260)
                     /locus_tag="PLJ11_03720"
     CDS             complement(76..1260)
                     /locus_tag="PLJ11_03720"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOO03809.1"
                     /translation="MGRFFSAEHDDIQKGLTTDVYFQRTEAILKAKGLLDVHTRSEVT
                     VADLPDAWPWAVLCGTEEVVRILEGREVDLYALPEGSLFTPRTRRGVKLPVASIDGPY
                     GQYCSLETPALGFICYASGVATMAARCKLAAGSLPVISFGVRRMHPAMAPVIDRSSFI
                     GGCDGVSSLKGAELVGKAASGTMPHSLVIMMGDQREAFKAFGEIIDPTVPRVALTDTF
                     FDEKAEALMAAEAMPGLRSVRLDTPGSRRGNMADIVREVRWELDLRGYTNVGVFVSGG
                     LDEIKIKELASAGANGFGVGTSISNAPTIDFAMDIVEKAGKPIAKRGKFAGRKYVFKC
                     PDCLEYEVSLRRDDPPKCSCGEEMRWAEVKVIEKGKRLVPEEPAEEVRRRALRQMERA
                     AL"
     gene            complement(1310..2581)
                     /gene="eno"
                     /locus_tag="PLJ11_03725"
     CDS             complement(1310..2581)
                     /gene="eno"
                     /locus_tag="PLJ11_03725"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOO03810.1"
                     /translation="MADVTITRVWSREVLDSRGNPTVEAQVEAGKVTVTAIAPSGAST
                     GTFEALELRDGGNRYSGKGVLKAVRNVRELISPRLKGMDVTDLKAVDEAMRELDGTDN
                     MGRLGGNATTAVSYAAAKAGAVLKGVELHEHLGQGSKVLPVPCMNVINGGKHAGGNLR
                     VQEFMVVPCGAPSFSEALRAGTEVYHALRQVLRKGMGPSSINVGDEGGFAPPFDTSRQ
                     ALDALLRAIEAAGYAPGKEVSLAMDAAASEFYSNGVYEVDGRRMAPGEIVDFYIDLSK
                     EYPLISLEDPVHEEGFLDMAELTRKVGTRLQLVGDDLFVTNVDRLRRGIEAKAGNAML
                     LKVNQIGTVSDAMRAAAMCFDHGYNVMVSHRSGESEDTTIADIAVALECGQIKSGAPA
                     RAERTAKYNRLLRIEEGLGGKAVFKGREAFL"
     gene            complement(2600..3541)
                     /locus_tag="PLJ11_03730"
     CDS             complement(2600..3541)
                     /locus_tag="PLJ11_03730"
                     /inference="COORDINATES: protein motif:HMM:NF017570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF835 domain-containing protein"
                     /protein_id="HOO03811.1"
                     /translation="MDDNAAILEIVTELISNAGYQPLTAIGGRECLAKVQSERPDLML
                     LDINMPDMDGWAVLRTLKEKGLTEGMKIMMLTATTEIGADIFGLQDVVTGYIRKPFTN
                     DELSQKVRKALGDQPEGRETSVLESERKGLRSLLRKRDEKGGVEEARTSAKMYELHRG
                     FSYLVKETKPHRSFEIFVDQVTHNIPGLCVTRQHPTIIRNQWGLEKTPIIWLSNQLGK
                     VYVNPTNIGILSDTIIRFVEKSGDSIVLIDGVEFLIVNNDFDKVLRMVHHVTEAVMEN
                     RSRLIISVDPRTLETRELALLERNMEIIEAEEARPHQ"
     gene            complement(3673..3822)
                     /locus_tag="PLJ11_03735"
     CDS             complement(3673..3822)
                     /locus_tag="PLJ11_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HOO03812.1"
                     /translation="MARFKEADERLLNKKVCLSCYARNAVKATRCRKCGYTSLRMKAK
                     ESRKA"
     gene            complement(3875..4843)
                     /locus_tag="PLJ11_03740"
     CDS             complement(3875..4843)
                     /locus_tag="PLJ11_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HOO03813.1"
                     /translation="MWLEIMQEIWTEKYRPRRLSDIVGQKEIVERLSSYVRSGNLPHL
                     MFAGPAGTGKTTSALAMAKEMYGEAWRENFIELNASDERGIDVVRGKIKEFARTAPIG
                     GASFKIIFLDEADALTQDAQAALRRTMERYSRNCRFVLSCNYSSKIIEPIQSRCAVFR
                     FRPLRAEDIKRYLQAIAQKEGIKIDDDALDAIVHVGEGDMRKSINSLQVAATMGGDVT
                     IDVVYRATGNARPDEVVDMVNTALAGDFSGARAKLDEMMVTYGLSGEDIIKQIHRSFY
                     DLDMPDREKIRLIDRTGEIEFRIVEGSNERIQLESLLAYLALMERD"
     gene            4931..5842
                     /locus_tag="PLJ11_03745"
     CDS             4931..5842
                     /locus_tag="PLJ11_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HOO03814.1"
                     /translation="MRCSKCSAPAVTYIRYNGSHLCPAHLLEYVERRVKKEVRSQLRL
                     EGETHIAVGASGGKDSQTALFLLTEILGRRRNVRVTAITVDEGIAGYRPETVPKVREL
                     CRELGVEHVVVSFEEVAGLSLDTMVARTDAREACTYCGVFRRRCLNITARELGVDVLA
                     LGHNLDDMAQSVLMNITRGDVERLARLGPHAHAQPGLVPRIMPLRTVPEKETYLYALL
                     SGLPFSDAECPYAVHALRNEYRQIIDGMEHRHPGTRHAILGSYDRLRPLMREGFPQAK
                     LRECACGEPTLNERCMACEMLERLKKG"
     gene            complement(5870..>6503)
                     /locus_tag="PLJ11_03750"
     CDS             complement(5870..>6503)
                     /locus_tag="PLJ11_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOO03815.1"
                     /translation="NVMTASTHKTFPGPNHGIVLGNDLTEDLGKRLSSAVFPGVTSSH
                     HLHAMAALAVTLAEWEIYGEGYAAQVCKNAKALGQALNELGMDVLCAHKGFTESHTIA
                     VNVAAHGGGAEAANLMEKANIIANKNMLPGDRSSVKPSGIRLGSQELTRVGMREGEMK
                     EVASLIHRVVVKKEAPEAVKKDVIALKRSFTKVRYCLHEGEEAYAYHKLV"
BASE COUNT         1090 a         2138 c         1939 g         1336 t
ORIGIN      
        1 gtcgaagctg atcctgaact ggtagtcgtg cagcagctcc agctcggtaa ggaacccctc
       61 ttgcatcctc gcacctcaga gggccgcccg ctccatttgc ctgagcgccc ggcgccggac
      121 ctcctccgcc ggctcctcgg gcaccaggcg cttccccttc tcgatcacct tgacctcggc
      181 ccaccgcatc tcctcgccgc aggagcactt gggcggatcg tcgcggcgta gggagacctc
      241 gtactccagg cagtccgggc atttgaacac gtacttgcgc ccggcgaact tccctcgttt
      301 ggcgatcggc ttcccggcct tctccactat gtccatggcg aagtcgatgg tgggggcgtt
      361 gctgatggac gtccccactc cgaacccgtt ggcccccgct gaggccagtt ccttgatctt
      421 gatctcgtcg agaccgccgg agacgaatac gcccacgttg gtatagccgc gcaggtcaag
      481 ctcccagcgg acctcacgga cgatatcggc catgttccca cgccgggagc ccggggtgtc
      541 cagcctgacg gagcgcagcc cgggcatcgc ctccgcggcc atgagggcct cggccttctc
      601 atcgaagaac gtgtccgtca gcgccaccct cgggaccgtc gggtcgatta tctcgccgaa
      661 ggccttgaac gcctcccgct ggtcgcccat cattatgacc agcgaatggg gcatggtccc
      721 gctcgccgcc ttgccgacca gctctgcacc cttgagcgat gacacgccat cgcatccccc
      781 tatgaaggag gaacggtcga tgaccggcgc catggcgggg tgcatccgcc tcaccccgaa
      841 cgagatgacg ggaaggctgc cggcggccag cttgcaccgg gcggccattg tggcgacccc
      901 gctggcatag catatgaagc ccagggcagg ggtctccagg gagcagtatt gaccataggg
      961 accgtcgatg gatgccacgg gcagcttcac ccccctccgg gtgcgcgggg tgaacaatga
     1021 tccctccgga agggcgtata ggtccacctc cctcccctcc aagatccgca ctacctcctc
     1081 ggtcccgcag agcaccgccc aaggccacgc atctggaagg tcggccaccg tcacctcgga
     1141 cctggtatgg acgtccagaa gccccttggc cttcaggatc gcctcggtgc gctggaagta
     1201 gacgtcggtc gtcaagccct tttggatgtc atcatgctca gcggagaaga acctgcccat
     1261 cgcatcacct gaacatgcgc taatcaccgt tttccatgat taaccttctt caaaggaagg
     1321 cctcgcggcc cttgaagacg gctttccccc ccagcccctc ctctatgcgc agcagcctgt
     1381 tgtacttggc cgtgcgctcc gcccgcgccg gcgccccgct cttgatctgg ccgcactcga
     1441 gggcgaccgc gatgtcggcg atcgtggtgt cctccgattc cccggagcga tggcttacca
     1501 tcacgttgta gccgtggtcg aagcacatcg ccgccgcccg catggcatcg gacaccgtcc
     1561 cgatctggtt caccttcagc agcatcgcgt tccccgcctt ggcctcgatc cccctccgca
     1621 gccggtcgac attggtgacg aacaggtcgt cccccacgag ctgcagcctt gtcccaacct
     1681 tgcgggtcag ttcggccatg tcgaggaacc cctcctcatg caccgggtcc tccaggctga
     1741 tgagcgggta ttccttgctc aggtcgatgt agaagtccac gatctccccg ggggccatcc
     1801 gcctgccgtc cacctcatag acgccgttgg aatagaactc gctggcggcg gcgtccatgg
     1861 ccagggacac ctcctttcca ggggcgtacc ctgcggcctc gatcgccctg agcagggcgt
     1921 ccaaggcctg gcggctcgta tcgaacgggg gggcgaaccc gccctcgtcc cccacgttga
     1981 tggacgatgg ccccatcccc ttcctcagga cctgcctcag ggcatgatag acctcggtgc
     2041 ctgccctcag cgcctcggag aagctggggg ccccgcaggg aacgaccatg aactcctgga
     2101 ccctcaggtt gcctccggca tgctttccgc cgttgatgac gttcatgcat gggaccggca
     2161 gtaccttgct gccctgtccg agatgctcgt gcagctccac gcccttcagg accgccccgg
     2221 ccttggccgc ggcgtaggag acggcggtgg tggcgttgcc gcccagcctg cccatgttgt
     2281 ccgtcccgtc aagctccctc atggcctcgt ccaccgcctt gaggtccgtg acgtccatgc
     2341 ccttgagcct gggcgagatg agctcccgga cgttcctaac ggccttgagc acgccctttc
     2401 ccgagtatct gttccctccg tcacgaagct cgagggcctc gaaggtcccg gtggacgccc
     2461 ccgaaggggc tatggcggtc acggtcacct ttccggcctc cacctgcgcc tccacggtgg
     2521 ggttgcccct ggagtccagc acctctctgc tccaaaccct ggttatcgtt acgtcagcca
     2581 tactgtgcag ctccgttgct cattgatgcg ggcgggcctc ttccgcctcg atgatctcca
     2641 tgttcctttc caggagcgca agctccctgg tctcgagggt ccgggggtcc acggatatga
     2701 tgagccggga acggttctcc atgacggcct cggtgacatg atggaccatc ctcagcacct
     2761 tgtcgaaatc gttgttgact atgaggaact ccacgccgtc tatgaggacg atgctgtcgc
     2821 cgctcttctc gacgaagcgg atgatggtgt cgctcaggat cccgatgttg gtggggttca
     2881 cgtacacctt ccccaactgg ttgctgagcc atattatcgg ggtcttctcg agcccccact
     2941 gattgcggat gatcgtaggg tgctgccggg tgacgcatag accagggatg ttgtgggtca
     3001 cctggtccac gaatatctcg aaggacctgt gcggcttggt ttctttcacc agatagctga
     3061 acccccggtg cagctcgtac atcttggcgc tggtgcgcgc ttcctccacg cctcccttct
     3121 cgtccctctt ccgaaggagg gagcgcagcc ccttgcgctc gctttcgagg accgacgtct
     3181 ccctgccctc gggctggtcc ccaagcgcct tcctgacctt ctgggagagt tcatcgttgg
     3241 tgaacggctt gcggatgtac ccggtcacca cgtcctgcag cccgaagatg tcggcgccga
     3301 tctcggtcgt ggccgtgagc atcattatct tcatgccctc ggtcagccct ttctccttca
     3361 aggtgcgcag gaccgcccag ccatccatgt ccggcatgtt tatgtccagg agcatcaggt
     3421 ccggcctctc cgactggacc ttggccaggc attcccggcc gccgatggcc gtgagaggct
     3481 gatagcccgc attggagatc agttcggtga cgatctccag gatggcggca ttatcgtcca
     3541 cgaccaagac ctttttgatt cgaattcccc cagtgcctag agcagtgagg catgcactcc
     3601 cctgattaaa aatcctatcc catggaatca gcttgggaga atggtgcgga ggaaggggtt
     3661 tgcggccctt gcttaggcct tgcggctctc tttggccttc atacgcaacg aggtgtatcc
     3721 gcacttgcgg cacctggtgg ccttgacggc gttacgggca tagcagctga ggcagacctt
     3781 cttgttcagc agcctttcgt cagcttcttt gaagcgtgcc atttttcccc tctcgccttg
     3841 aaatagggag ccgatataaa tacgttgccg gtccctagtc cctttccatc agcgcgaggt
     3901 acgccagcag ggattccagc tggatgcgtt cattgctccc ttccacgatg cggaactcta
     3961 tctcccccgt gcgatcgatc agcctgatct tctccctgtc aggcatgtcc aggtcgtaga
     4021 acgaccggtg gatctgcttt attatgtcct ccccgctcag tccataggtg accatcatct
     4081 cgtccagctt tgcccttgcg cctgagaagt ccccagccag ggcggtgttc accatgtcca
     4141 ccacctcgtc cggccgggcg ttccccgttg cccgatagac gacatcgatg gtgacatccc
     4201 ctcccatcgt ggcggccacc tgcagggagt tgatggattt gcgcatgtcg ccctcgccca
     4261 cgtgcacgat ggcgtccagg gcgtcgtcgt cgatcttgat tccctccttc tgggcgatgg
     4321 cctggagata gcgtttgatg tcctcggccc ttaggggacg gaaccggaat acggcgcagc
     4381 gggattggat gggctcgatt atcttggagg aatagttgca ggagagcacg aaacggcagt
     4441 tccggctgta gcgttccatg gtcctccgca gcgcggcctg cgcatcctgg gtgagcgcat
     4501 cggcctcatc gaggaatatt atcttgaagg aggccccgcc tatgggcgcg gtgcgggcga
     4561 actccttgat ctttccacgg acgacgtcga tgccacgctc gtccgaagcg ttcagttcga
     4621 tgaagttttc gcgccacgcc tccccgtaca tctcctttgc catagccagg gcggatgtcg
     4681 tcttgcctgt gcccgcgggc ccggcgaaca tcagatgggg cagattgccg gagcgcacgt
     4741 atgaggaaag cctctccacg atctccttct ggccaacgat gtccgacagc ctgcgtgggc
     4801 gatacttctc ggtccaaatc tcctgcatga tctccagcca catcaaagat gtaactaagt
     4861 taaaaggtta tctggggacg atcggacgta ttcccgaaca tttaataccc cggccggcgt
     4921 gaacggagcg atgcgctgct ccaagtgctc cgcccccgcg gtgacctaca tccgctacaa
     4981 cgggtcccat ctatgccccg cgcatctcct cgagtacgtg gagaggaggg tcaagaagga
     5041 ggtgcgctcc cagctcagat tggagggaga gacgcacatc gccgtgggcg cttccggagg
     5101 aaaggatagc cagacggcgc tcttcctatt aacggagatc ctcggccgtc ggaggaacgt
     5161 gcgcgtgacc gccatcaccg tggacgaagg gatagcgggc taccgccccg agacggtccc
     5221 caaggtccgc gagctctgcc gcgaactggg ggtggagcat gtggtggtgt cgttcgaaga
     5281 ggtggcaggc ctgagcttgg atacgatggt cgcccggacc gacgcccggg aggcgtgcac
     5341 ctactgcggt gtctttcgaa ggcgatgcct caacataacg gcaagggagc tgggcgtgga
     5401 cgttctggca ttggggcaca acctggacga tatggcccag tcggttctta tgaacatcac
     5461 ccggggcgac gtggaacggt tggcccgcct ggggccgcat gcccacgccc agccaggcct
     5521 ggtgcccagg atcatgccct tgcgcaccgt cccggagaag gagacgtacc tttatgccct
     5581 gctctcgggc cttcccttct cggatgccga gtgcccctat gcggtgcacg ccctgcgtaa
     5641 cgagtaccgg cagataatag acgggatgga acacaggcac cccggcacca gacatgccat
     5701 cctggggagc tacgaccgcc tcaggccttt gatgcgggag ggattccccc aggccaagct
     5761 ccgggagtgc gcctgcgggg aaccgaccct gaacgagagg tgcatggcct gtgagatgct
     5821 ggaaaggctg aagaaaggtt gaggggggtt tggaaagggg cctgagaggt cataccaact
     5881 tatggtaggc gtacgcttcc tcgccctcgt gcaggcagta gcgcaccttg gtgaaggagc
     5941 gcttcagggc gatgacgtcc ttcttgaccg cctcgggggc ctccttcttg accacgacgc
     6001 gatggatcaa ggaggccacc tctttcatct cgccttccct catgcccacc ctggtaagct
     6061 cctgggagcc gaggcggatg ccggacggtt tcacggagga cctgtccccg ggcagcatgt
     6121 tcttgttggc gatgatgttg gccttctcca tgaggttggc cgcctcggcg ccgccgccgt
     6181 gcgcagcgac gttgacggcg atggtatggc tctcggtgaa gcccttgtgg gcgcagagca
     6241 cgtccatgcc cagctcgttc aacgcctggc ccagggcctt ggcgttcttg catacctggg
     6301 cggcgtaccc ctcgccgtag atctcccatt ccgccaaggt caccgcgagc gcggccattg
     6361 catgcaggtg gtggctcgag gtcaccccgg ggaacaccgc agaactgagc ctcttgccca
     6421 ggtcctcggt gagatcgttc ccaagtacga tgccgtggtt gggccccggg aacgtcttgt
     6481 gggtcgaggc ggtcatgaca ttc
//
LOCUS       DAOSYN010000061         6443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10197_length_6443_cov_3.900595, whole genome shotgun sequence.
ACCESSION   DAOSYN010000061 DAOSYN010000000
VERSION     DAOSYN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10197_length_6443_cov_3.900595"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..777
                     /locus_tag="PLJ11_03755"
     CDS             262..777
                     /locus_tag="PLJ11_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOO03816.1"
                     /translation="MARKPGRMYRRLTGQAYTRREYMGGVPALRINTFDLGTPNGDFP
                     ITVNLKVKETCQIRHIAMEAARIACNRVMSKTPGNNYHIKFRIYPHHVLRENKLATGA
                     GADRVSSGMRGAFGKNVGTAARVHAGQVVLTIRVPAANFNSAKEALWKASLKLPTPCF
                     IEVEKGAELVR"
     gene            893..2146
                     /locus_tag="PLJ11_03760"
     CDS             893..2146
                     /locus_tag="PLJ11_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO03817.1"
                     /translation="MGFISNYLFRRFKAPDVLILMFLGIILGPGGIGLIGSEAAEGLE
                     AFTPYVAAAALAIIMFQAGMDLAIGDVVKSFSKSLIQTIIAFLASMLITALICLGLTD
                     WNLETCLLLGSIVGGTSGAIVIPIISGLRISTNLKMVLTLESAITDVLVIVVATSLIV
                     FMSQDTADIMGAVELLVSSFMVSTMIGIIAGVLWTRLLQRLSGQPFSYMITIAAMLMV
                     YSITDILVEKAGGGAIAVLVFGLTLGNWEEISRVLRQKGEQFALGEKVKRFHDEISFL
                     IRTVFFVYLGLVITTIHFTAWYVLLGLFVFFGLLIARYISLLLANRYIGLDASDEMGL
                     FYMMPRGLAAAVMASIPFSYPLVFSNEVATAILGVTVVVVLLSTVLASLGAFYMELHA
                     KRNGLNGEGERETSENGSEGGEDDL"
     gene            2143..3141
                     /gene="dph2"
                     /locus_tag="PLJ11_03765"
     CDS             2143..3141
                     /gene="dph2"
                     /locus_tag="PLJ11_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HOO03818.1"
                     /translation="MMFNIELERAIAEIRIRGARKVGLQMPDGLRPRARAFASELEEA
                     TGCVVVILGDPCYGACDVHQEPGLDLLVHIGHAPIPDLRPSSPVLFLEVRIDLDVRKG
                     LLESSRRIRDRVGLVATAQHLHLLPEAAEALREMGRIPLIGMGDRRTVAPGQVLGCNI
                     TSALAIRSEVEQYLFLGSGYFHPMAVSLGTGLPVLCIDPHRGPVDDVATLTEKVVRQR
                     YAAMAKAKDAKDWLVILCDKPGQRRARTALRCRELLRERGMKADIVQLTEVRAEALLP
                     YRAEAVVSTACPRLAIDDGPHYPIPLLTPSELEMVLGLRGMDDYRLDQITEGDTSG"
     gene            3184..3945
                     /gene="mtxX"
                     /locus_tag="PLJ11_03770"
     CDS             3184..3945
                     /gene="mtxX"
                     /locus_tag="PLJ11_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HOO03819.1"
                     /translation="MLGPEGIFERAIASHARVGIGKGDDPRKALRSARSAEAKGYADV
                     VVYDSAKGMVAALCTGEVDAVVRGDLGANTTMAEVKRQFSLDHLLRAALMKPRGGRMF
                     FLAPVGVDEGWEVRAKMEFIRRSARLFEQLGEEMRVGVLSGGRLEDIGRNANVDRTIR
                     EALEVERLGKEEGFNVEHCQILIEDAIKVKNLIIAPDGISGNIIFRTMHLVDGCVSMG
                     APILNLDKVFVDTSRAKRCYVDSIALASALAFQGK"
     gene            3981..4463
                     /locus_tag="PLJ11_03775"
     CDS             3981..4463
                     /locus_tag="PLJ11_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydropterin synthase family
                     protein"
                     /protein_id="HOO03820.1"
                     /translation="MRLEIDGEFAGIRFSACHFIAGHHKCGRLHGHTYVVSLRLQGDM
                     GKDGMVMDFIPLKKELRSIVEELDHRVLVPANSKRITVALGEEVFIKADGKRYVLPKE
                     DVVLLPTEESSAEELCTFILRKVMENVDFPPNIWEVEVGVHEELGQSAWASKRLEAKG
                     "
     gene            4460..5131
                     /gene="queC"
                     /locus_tag="PLJ11_03780"
     CDS             4460..5131
                     /gene="queC"
                     /locus_tag="PLJ11_03780"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOO03821.1"
                     /translation="MKAICLLSGGLDSTVTLACALRQGYEVTALTINYGQRHIRELDA
                     ARAVAAHYKLPHVLMDFPLVGFRSALTDESIPVPDRGIDEIGYDIPQTYVPARNIVFL
                     SLAAGMAESIDASAVFIGANVIDYSGYPDCRPEFFQAFEAMLAVGTKRGVLGHPVRIE
                     HPILTKSKAEIVKMGLKLEAPLHLTWSCYRGGERACGHCDSCVLRLKGFREAGVEDPV
                     PYEGR"
     gene            5131..5760
                     /locus_tag="PLJ11_03785"
     CDS             5131..5760
                     /locus_tag="PLJ11_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03822.1"
                     /translation="MLVNEMFLSIQGEGGTMGLPTAFVRLSGCNLNCHWCDTRYAREG
                     GAELSVSDVMTAVGAYRVRHVCLTGGEPLLQGETPELIASLLTEGKHVTLETNGSLPI
                     GGLPAHPDLLVSMDYKCPSSGEEGSMLIENLSLLRRKDQLKFVVSDVRDMERAEKVLL
                     RYHPDCSAIFTPVGGLTLGPVADFVLSHRLEVRVLPQLHKLIWGERRGV"
     gene            5919..>6443
                     /locus_tag="PLJ11_03790"
     CDS             5919..>6443
                     /locus_tag="PLJ11_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03823.1"
                     /translation="MTELRSSCSQDSGAYGGYEAGRIFTSHFNRLALDPSTPERVEVL
                     DTTLRDGEQTPGVALSIDDKVRIAQLLDELGVDVIEAGFPRTSKGEQEAIRRIASLGL
                     KAKVCGLARCSREDVDSAVDSGVDYVHAFIATSEAHMKHKLKMTREQVKARAVDAVEY
                     ARSRGVTVEFSCEDG"
BASE COUNT         1289 a         1877 c         2049 g         1228 t
ORIGIN      
        1 cccaacaccc tccgaggatc gggtacccag tcctatccat cttcccaagc ttgggccaag
       61 gcaggctgag ggggcccaac ccacccccgt cattgtactg gatgcgctgc atggccgggc
      121 ctaatgggag acggccccct caatcgcccc tccccactcg gtcaaggcca taccctccca
      181 gttgcccttt ccatcagtct tatataggaa aataggaatg tagccatccc gtcgcattaa
      241 tgcaatcaca ggtggtatga tatggccaga aagccgggaa ggatgtacag gaggctcaca
      301 gggcaagcct atacccgaag ggaatacatg ggtggggttc cagccctgag gatcaacact
      361 ttcgatcttg ggacccccaa cggggacttc cccatcaccg tcaacctcaa ggtcaaggaa
      421 acctgccaga taaggcacat tgccatggag gccgcccgta tcgcctgcaa cagggtcatg
      481 tccaagaccc cgggcaacaa ctatcacatc aagttcagga tctacccgca ccacgtgctc
      541 cgggagaaca agctcgccac cggtgctggt gccgaccgtg tgtccagcgg gatgagggga
      601 gccttcggca agaatgtggg caccgccgcc cgagtgcatg ccgggcaggt cgtgctcacc
      661 atcagagtgc ccgcggccaa cttcaacagc gccaaggagg cgttgtggaa ggcgtccctc
      721 aagctgccca ccccctgctt catcgaggta gagaagggcg ccgagctggt gcggtaggcc
      781 gcgatcatca aatcccttcc ctgcaattaa tttaaatccc tgacccttct actccatccg
      841 atatacattg gacgctgaca ttctattcct ggctataggt gccattatat tcatggggtt
      901 catctctaat tatctgttca ggcgcttcaa ggcccccgac gttctgatcc tcatgttcct
      961 cggcatcatc ctgggccctg gaggcatcgg cctgataggc tctgaggcgg cagagggcct
     1021 cgaggctttc accccctacg tggccgctgc agcgctggcc atcatcatgt tccaggcagg
     1081 tatggacctg gcgatcgggg acgtggtcaa gtccttcagc aaatcactga tccagacgat
     1141 catcgccttc cttgcctcca tgctgataac cgcgctcatc tgcctcggtc tcaccgattg
     1201 gaacctggag acctgcctat tattgggctc catcgtgggt gggaccagcg gggccatagt
     1261 catcccgatc atctccggcc tcaggatatc caccaacctc aagatggtgc tcacgctcga
     1321 atcggccatc accgatgtgc tggtgatcgt ggtcgccacc tcgctaatag tatttatgag
     1381 ccaggacacg gcggacataa tgggtgcggt cgagctcctt gtaagctcat tcatggtctc
     1441 cacgatgatc gggatcatcg ccggggtgct gtggacaaga ctgctccagc ggttgagcgg
     1501 acagcccttt tcatacatga tcaccatagc ggcaatgctc atggtctact cgatcaccga
     1561 tatcttggtc gagaaggcag ggggcggggc gatcgcggtg ctggtgttcg gcctcaccct
     1621 cggcaattgg gaagagatat ctagggtgct gaggcagaag ggggagcagt tcgccctggg
     1681 ggagaaggtg aagcgcttcc acgatgagat atcattcctc atccggaccg tcttcttcgt
     1741 ctatctgggg cttgttatca ccaccatcca tttcaccgcc tggtacgtgc tgctgggact
     1801 gttcgtcttc ttcggcctgc tcatcgcccg gtacatctcc cttctgttgg ccaacaggta
     1861 catcggcctc gacgccagcg atgagatggg gctgttctac atgatgccca ggggcctggc
     1921 ggccgcggtc atggcctcca tccctttctc ctatcctctc gtgttcagca atgaggtcgc
     1981 tacggccata ctaggggtga cggtcgtcgt ggtgctgctc tccaccgtcc ttgcctctct
     2041 gggagcgttc tacatggagc tgcatgccaa gcgcaatggg ctcaatggcg aaggggaaag
     2101 ggagacctcg gagaacggct cagaaggcgg cgaggacgat ctatgatgtt caacatcgag
     2161 cttgagcgcg ccatagcgga gatcaggata aggggggcga ggaaggtcgg gcttcagatg
     2221 ccggacgggc tcaggccgag ggcaagggcg ttcgccagtg aactggagga ggccaccgga
     2281 tgcgtggtgg tcatccttgg cgacccatgc tacggcgcat gcgacgtcca ccaggagccc
     2341 ggcctggacc tccttgtcca cataggccat gccccgatcc cggacctcag gccatcgtcc
     2401 cccgtgctct tcctcgaggt ccggatcgac ctcgatgtca ggaaggggct gctggaaagt
     2461 tcaaggcgga tcagggacag ggtggggttg gtggccaccg cccagcacct tcacctcctc
     2521 cctgaggcgg cggaggcctt gagggagatg gggcggatcc ctctgatcgg gatgggagat
     2581 aggcggaccg ttgccccagg ccaagtgctc ggatgcaaca tcacctccgc cctggccata
     2641 aggagcgagg tcgaacagta cttattcctt ggaagcggat actttcaccc aatggccgtt
     2701 tccctgggca cggggctgcc agtcctctgc atcgatcccc accgcggccc ggtcgatgac
     2761 gtggccacgc tcacggaaaa ggtcgtccgg caacgctatg cggccatggc gaaggcgaag
     2821 gacgccaagg actggctcgt catcttgtgc gacaagcctg ggcaaaggcg ggcccgaacc
     2881 gcactccggt gcagggagct cctgagggag aggggaatga aggcggacat cgtacagctc
     2941 accgaggtcc gcgcggaggc gctgcttccc tatcgggcgg aggcggtggt gagcaccgcc
     3001 tgtccccgcc tcgcgatcga cgatggcccc cattacccga tcccgctgct caccccgtcg
     3061 gagctggaga tggtgctcgg cctccgggga atggacgatt accggttgga tcagataact
     3121 gaaggggaca cctccgggta ggacaatgaa gctattatat ccaaaagggc ggatagcctc
     3181 cccatgcttg gaccggaggg catcttcgaa agggcgatcg ccagccatgc aagggtcggc
     3241 attggaaagg gggatgaccc ccgcaaggcc cttcgcagcg ccaggtccgc tgaggccaaa
     3301 gggtacgcgg acgtcgtggt ctacgattcc gccaaaggga tggtagccgc cctctgcacc
     3361 ggcgaggtgg atgcagtggt gcgcggggac ctcggcgcga acaccaccat ggctgaggtc
     3421 aagcggcagt tctcgttgga ccatctcctg cgggcggcgc tcatgaagcc tagaggcggg
     3481 cgcatgttct tcctcgcccc ggtgggggtc gacgaggggt gggaagtgag ggcgaagatg
     3541 gagttcatcc gtcgctccgc ccgcctgttc gagcagctgg gagaggagat gagggtcggg
     3601 gtcctgtccg gagggcgcct ggaggacatc gggcgcaacg ctaacgtaga caggacgata
     3661 agggaggcct tggaggtgga gcggctgggc aaagaggaag ggttcaatgt ggaacattgt
     3721 cagatcctga tcgaggatgc gataaaggtg aagaacctga taatcgcccc ggacggcatc
     3781 agtggcaaca tcatcttccg caccatgcac ctggtcgatg gatgcgtttc catgggggcg
     3841 cccatactca acctggacaa ggtgttcgtg gacacgtcca gggcgaagag gtgctatgtc
     3901 gactccatag ccctcgcctc cgccctggca tttcagggaa aatgattatt gggcctcgga
     3961 gcaatatgaa gggagatcat atgcggctag agatcgacgg ggagttcgcg gggatcaggt
     4021 tctccgcatg ccatttcatc gccggccatc acaagtgcgg tcggttgcac ggacatacat
     4081 atgtcgtcag cctgaggctg caaggggaca tgggcaagga tggcatggtc atggacttca
     4141 ttcccttgaa gaaggaactc aggagcatag tcgaggagct cgaccaccgg gtcctggttc
     4201 cggccaactc aaagaggatc accgtggcgt tgggcgaaga ggtcttcatc aaggccgatg
     4261 gcaagaggta cgttctgccc aaggaggacg tcgtcctcct gccgaccgag gagagcagcg
     4321 cggaggagct gtgcaccttc atcctaagaa aggtcatgga gaacgtggac ttccccccca
     4381 acatctggga ggtggaggtg ggggtgcatg aggagttagg gcagagcgca tgggccagca
     4441 agagattgga ggcgaaggga tgaaggccat ctgcctgctc tccggggggc ttgactcgac
     4501 cgtcaccctc gcctgcgcgc tacgtcaggg atatgaggtc accgccctga ccatcaacta
     4561 cggtcagagg cacataaggg aattggatgc ggccagggcg gtagccgccc attacaagtt
     4621 gccccacgtg ctgatggact tccccctcgt cggcttccgt agcgcgctca cggacgagtc
     4681 catcccggtg cccgaccggg gcatcgatga gatcgggtac gacattccgc agacgtacgt
     4741 cccagccagg aacatagtgt tcctcagcct ggccgcaggt atggcggaga gcatcgacgc
     4801 cagcgcggtc ttcatagggg ccaacgtcat cgattactcg ggctaccctg actgccggcc
     4861 cgagttcttc caggccttcg aggccatgct tgcggtgggg accaagcggg gcgtgctcgg
     4921 ccatccagtg cgcattgagc atcctatttt aacgaagagc aaggcggaga tagtgaagat
     4981 gggcctcaaa ttggaagcgc ccctccacct tacctggagc tgctatcgag gcggggaaag
     5041 ggcgtgcggc cactgcgatt cctgcgtgct taggctgaaa gggttcaggg aggccggggt
     5101 ggaggaccct gtgccctacg agggccgctg atgttggtca acgagatgtt cctctccatc
     5161 cagggggagg gagggacgat gggcctgccg acggcgttcg ttcgcctgag cgggtgcaat
     5221 ctcaactgcc attggtgcga cacccgctat gccagggagg gcggggcaga gctgtccgtc
     5281 tcggacgtca tgaccgccgt cggcgcctac cgcgttcgac acgtatgcct caccggagga
     5341 gagccgctgc tgcaagggga aacccccgag ctgatcgcga gcctgctcac tgagggaaag
     5401 catgtcaccc tggagaccaa cggctccctc cccataggcg gcctcccggc ccacccggac
     5461 ctgctggtca gcatggacta caagtgcccc tcctcagggg aggagggctc gatgctgatc
     5521 gagaacctgt ccttgctgag gcgcaaggac cagttgaagt tcgttgtatc cgacgtccgg
     5581 gacatggaga gggcggagaa ggtgctcctc agataccatc ccgactgctc tgcgatcttc
     5641 accccggtgg gcgggctgac cctggggccg gtggcggact tcgttctcag ccacaggctg
     5701 gaggtcaggg tgctcccgca gctgcataag ctcatctggg gtgagaggcg cggggtctag
     5761 gcgtgccatg atctagcaat gattaaataa tatgttccta gttccctacc ccatacattc
     5821 ggggtagtgc ggtgagcatc aatatcgtgg acggacacgg ccttttgcgt tactaagtca
     5881 tctcatcgcc gcgaccttgg atgtgagggt tggttatcat gaccgaactt cgatcgtcgt
     5941 gcagtcagga ctccggcgcc tatggtgggt atgaggcagg caggatattc accagtcact
     6001 tcaaccgctt ggccttggac ccctccaccc cggaaagggt agaggtgctt gacaccaccc
     6061 ttcgcgacgg agagcaaaca ccaggcgtag cgctctccat cgacgacaag gtgcgaatag
     6121 cccagctgct cgacgaactg ggggtggacg tgatcgaggc cgggttcccc cggacatcca
     6181 agggagagca ggaggcgata cgaaggatag cctccctggg gctcaaggcc aaggtgtgcg
     6241 gccttgccag gtgttccagg gaggacgtcg attccgcggt ggacagcggc gtggactacg
     6301 ttcacgcctt catcgccacc tccgaggctc acatgaagca taagctcaag atgacgaggg
     6361 agcaggtcaa ggcccgcgcg gtcgatgccg tggagtacgc ccgctcccgc ggggtgacgg
     6421 tggagttctc ctgcgaggac ggc
//
LOCUS       DAOSYN010000062         6414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10252_length_6414_cov_4.637993, whole genome shotgun sequence.
ACCESSION   DAOSYN010000062 DAOSYN010000000
VERSION     DAOSYN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10252_length_6414_cov_4.637993"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLJ11_03795"
     CDS             complement(<1..555)
                     /locus_tag="PLJ11_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01210 family radical SAM protein"
                     /protein_id="HOO03824.1"
                     /translation="MRSVDPTVPVTVWKEDDVVDGRRERALVAILRTRGCHWWRRGGC
                     SMCGYNVESAEGIGTEELSIQLKAVLARYEGERMVKLYTSGSFLDPDEVPPEMRKRFL
                     RSFDGAKRVLFESRPEFVTPDVLEELPDHAAVALGLESASDTVLRCSVNKGFSTRDYV
                     RAAEALQARGLPVRTYVLLKPPFLT"
     gene            665..2032
                     /locus_tag="PLJ11_03800"
     CDS             665..2032
                     /locus_tag="PLJ11_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO03825.1"
                     /translation="MARKVVVIGGGAAGMTAASTARGHGAGEITLITEDEHVAYSPCA
                     IPFVVEGRIKDFQDIVMHTPEFYRRERGIDVLVKTSVDKVDEEARRVMTKDGRSFEYD
                     ALVIATGGTVFIPPVEGRELKGVFPVRGISDGQAIMERLPMVHQAVVAGAGVIGLEMA
                     VALRARGKEVTVVEMFDQVVPRILDRDMAQLVQAHCEALGIRFILGAPLSCIEGEGSV
                     TGVTAGRHQVPCQMVIMATGVRANLRIPEQMGLEIGPLGGVRVSPSLQPYRKGRLVPD
                     VYLAGDVVMCQSAVLAGPTMSQLGSTAVRQGRVAGIRAAGGSATFPGTASPWISVIGD
                     MHVGGTGMSRSLAEYYGVKVAEATGVGSSRARYYPGGKDITVKLLADAGSGRLIGGQI
                     VGGEDVAGRVNWITAAILEGVTAGAFVERFEGSYCPPTSMVVDTVCAAAERLEKVLRA
                     QGKSV"
     gene            2063..3121
                     /locus_tag="PLJ11_03805"
     CDS             2063..3121
                     /locus_tag="PLJ11_03805"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOO03826.1"
                     /translation="MDLEERYALIARNTEEIITSEDLREVLRSKSSPTGYIGFEPSGL
                     VHIGWMVTAQKVRDFVDAGFSFIIFLADWHAYINDKLGGDIGNIRTCAEYMKDCFEAL
                     GLPRDKVEYRLASEIMDDIKYWEMVMKVGKASSLQRVKRAMTIMGRSEDEAEVDASKT
                     LYPLMQAADLIFMDVDVAYAGLDQRRAHMLAREAAEKMGVKKAVAVHTPLLPGLKGGN
                     RMDPVSAKMSKSDPDGSVLIHDAPEDIRRKLSKAYCPPEAEGNPVLAICRYIIFQRQD
                     VMRIERPERFGGNLEVKGYDELERTYLAGKLHPMDLKNGTATALAEALAPAREYFQRK
                     PENLERMRGVLAEVKKLR"
     gene            complement(3124..4068)
                     /locus_tag="PLJ11_03810"
     CDS             complement(3124..4068)
                     /locus_tag="PLJ11_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOO03827.1"
                     /translation="MLDDIIAKLSSAEKLILVHGNADPDALGSAYALAKAFPPADILP
                     AEGLDRTSKNVAARLGIELLARPQRSYPLIVILDTSSPEQLGDYGQMQGDMIIIDHHC
                     PTNKWAERGTYLCDDSKRSCAEVVLDLIERAGRPISREMALGLCAGILTDTGHFHFAN
                     PASLRAFARLMEGADVSMEEVLAITDSPTDISERVAQMKGGQRMRFERMGDHIVAISQ
                     GSAFESSVCKALLSLGADIAFVASQRNEGFRLSARARQELVRKGLHLGQLLGGVGEET
                     DSDGGGHGGAAGLVGIGDAEAILNICMQKALEFLRELR"
     gene            complement(4138..4518)
                     /locus_tag="PLJ11_03815"
     CDS             complement(4138..4518)
                     /locus_tag="PLJ11_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HOO03828.1"
                     /translation="MDEISPKLQNQIAQFQQLQQQLQTVMGQKIRMDAMLKEMEMTIE
                     ELKKTPQDATVYRNVGSLMIKVADIPALLKELEEDKETTEVRIKSLDRQEKMLKEKFQ
                     NVQEQLSRAIGAQGAPLRMDAEDV"
     gene            complement(4511..4753)
                     /locus_tag="PLJ11_03820"
     CDS             complement(4511..4753)
                     /locus_tag="PLJ11_03820"
                     /inference="COORDINATES: protein motif:HMM:NF011470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HOO03829.1"
                     /translation="MFRATLVVRSPFASNVAKAIGPEAGREIPRTRAEVHAPQGSVTL
                     RLDADDLPALRAALNSYIRWMCVAEKMSETVGDIDG"
     gene            complement(4755..4892)
                     /locus_tag="PLJ11_03825"
     CDS             complement(4755..4892)
                     /locus_tag="PLJ11_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HOO03830.1"
                     /translation="MNYKCAQCEKTTRSNLNQVGIQCESCGSKLFYKERPNVKKVIKG
                     R"
     gene            complement(4889..5164)
                     /locus_tag="PLJ11_03830"
     CDS             complement(4889..5164)
                     /locus_tag="PLJ11_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HOO03831.1"
                     /translation="MAKRTMKAGSSGRFGTRYGVVTRKLTRDIEKQQRSNFECPSCHH
                     MSVTRVASGIWACRHCETKFAAACYAPRIREAVSKELLAAHGKEQTP"
     gene            complement(5170..5943)
                     /gene="rrp42"
                     /locus_tag="PLJ11_03835"
     CDS             complement(5170..5943)
                     /gene="rrp42"
                     /locus_tag="PLJ11_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HOO03832.1"
                     /translation="MARSVVSEIKRDHIHRLIADGRRVDGRAWDQYREIMVQTNVVET
                     AEGSARVKIGNTEVIVGLKMTVGEPFPDTPNRGVLSTNAELIPMASPSFESGPPDENS
                     IELARVVDRGIRESQMIDLERLCVEPGKKVWINFLDIYVLDFDGNLFDACNLGAVAAI
                     KSATVPAKANGLGEDFPMPVTCTPVAVTSVQIENSILVDPTLDEEKVAAARLTVTTDD
                     NGDLRAMQKGLSGALTLEQVRTAIDTSRRLGAEIRKLIG"
     gene            complement(5946..>6414)
                     /locus_tag="PLJ11_03840"
     CDS             complement(5946..>6414)
                     /locus_tag="PLJ11_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504198.1"
                     /note="involved in the 3' to 5' degradation of a variety
                     of RNA species; forms a trimer of heterodimers (hexamer)
                     with Rrp42; Rrp41 is the catalytically active subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HOO03833.1"
                     /translation="SEAMEYVVFTENFPRTSIDVYIEVLQANAGTRCAGLTAASVALA
                     DAGIPMKDVVPAVAVGKVNDQVVLDLNKEEDNFGQADLPIAIIPRTGEVLLMQMDGHM
                     TQAEFDRGLEMGVRACSEIYRLQKDALRRRYAVVSGEGEEETKGLVHAMTEEA"
BASE COUNT         1160 a         2046 c         1991 g         1217 t
ORIGIN      
        1 ggtgaggaag gggggcttca gcaggacata ggtcctcacc ggcagtccgc gcgcctgcaa
       61 agcctccgcg gccctgacgt agtcccttgt gctgaagccc ttgttgacgg agcatctcag
      121 cacggtgtcg gaggcgctct ccagccccag ggcgaccgcc gcatggtctg gaagctcctc
      181 gagcacatcg ggcgtcacga actcggggcg gctctcgaac agcacccgtt tcgccccatc
      241 gaaggacctc aggaagcgct tccgcatctc cggggggacc tcgtcagggt cgaggaagct
      301 gcccgacgtg tagagcttca ccatccgctc gccctcgtac cgagcaagca cggccttgag
      361 ctggatggac agctcctccg tcccgatgcc ctccgcactc tcgacgttgt agccgcacat
      421 ggagcaccca cccctgcgcc accaatggca gccacgggtg cggaggatgg ccaccagcgc
      481 cctctccctc cggccgtcca ccacgtcgtc ctccttccat accgtcaccg gcacggtcgg
      541 gtccacgctc ctcacgctac caccatgtga gggatggtca accccccctt actattttat
      601 tgctgccctg ggggacgcaa cattttatat cctcccgtcc ggattcgcgg ccaggagagg
      661 catcatggcc aggaaggtcg ttgtcatagg agggggggcg gcaggcatga ccgccgcctc
      721 caccgcaagg gggcatgggg cgggcgagat caccctcatc accgaggacg agcacgttgc
      781 atactcgccc tgcgccatac ccttcgtggt ggaagggagg atcaaggact tccaggacat
      841 agtgatgcac acccccgagt tctaccggag ggagaggggg atcgacgttc tcgtcaagac
      901 ctcggtggac aaggtggatg aggaggccag gagggtgatg accaaggatg gccggtcgtt
      961 cgagtacgat gcgctggtca tcgccaccgg cgggacggta tttatccccc cagtggaggg
     1021 ccgagagctc aagggcgtgt tcccggtgag ggggatatcc gacggccagg ccataatgga
     1081 acggcttccc atggtccacc aggcagtggt ggcaggggcg ggcgtgatcg gcctggagat
     1141 ggccgttgcg ctgcgcgcaa ggggcaagga ggtcaccgtg gtggagatgt tcgatcaggt
     1201 ggtcccccgc atcctggaca gggacatggc ccagctggtg caggcgcact gcgaggccct
     1261 tggcataagg ttcatcctag gggctcccct ctcctgcatc gagggggaag ggagcgtcac
     1321 cggcgtcacc gccggcaggc accaggtccc gtgccaaatg gttatcatgg ccacgggggt
     1381 gcgggccaac ctccgcatcc cagagcaaat gggcttggag atcggacccc tcgggggcgt
     1441 ccgggtgagc ccgagcctgc agccctatcg caagggacgg ctcgtgcccg atgtctatct
     1501 cgcaggggac gtcgtgatgt gccaaagcgc ggtcctggcc ggccctacga tgagtcagct
     1561 cggctccacc gccgtcaggc agggcagggt ggccggtatc cgggccgctg gcggcagcgc
     1621 cacattccct gggaccgcca gcccttggat cagcgtgatc ggggacatgc acgtcggagg
     1681 cactggtatg tcccgctccc tcgccgagta ctatggggtc aaggtcgcag aggccacggg
     1741 cgtgggctcc tcccgtgcca ggtactatcc cgggggtaag gacataactg tgaagctcct
     1801 agcggacgcc gggagcgggc ggctcatcgg cgggcagata gtgggcggcg aggatgtggc
     1861 aggaagggtg aattggatca ccgcggccat tctggaaggc gtgacggcag gggccttcgt
     1921 tgaaaggttc gagggctcat actgccctcc cacctcgatg gtcgtagata ccgtatgcgc
     1981 ggcggcggag aggctggaaa aggtgctgcg ggcccagggc aaatctgttt aataccgtgt
     2041 gcctcattcg gccctgagca tcatggacct agaggagagg tacgccctaa tagcccggaa
     2101 caccgaggag atcatcacct cggaggacct gagggaggtc cttcggagca aatcctcccc
     2161 cactggctat atcggcttcg agccctcggg cctggtgcac atcggctgga tggtcaccgc
     2221 gcagaaggtt cgggacttcg ttgacgcggg cttctccttc atcatattcc tggccgattg
     2281 gcacgcctac atcaacgata agttgggcgg ggacataggc aacatccgga cctgcgccga
     2341 gtacatgaag gactgcttcg aggccctcgg gctgccgagg gacaaggtgg aatatcgtct
     2401 ggcatcagag atcatggacg acataaaata ttgggagatg gtcatgaagg tcgggaaggc
     2461 ctcctccctg cagcgcgtga agcgggcgat gaccattatg ggccgaagcg aggacgaggc
     2521 ggaggtcgat gccagcaaga ccctctaccc cctaatgcag gcggccgacc tgatcttcat
     2581 ggacgtggac gtggcctacg ctggcctcga ccagcggagg gcacacatgc tggcaaggga
     2641 ggccgcggag aagatggggg tcaagaaggc ggtggcggtc cacacacccc tgcttcccgg
     2701 gctcaagggc gggaaccgca tggaccccgt gagcgcgaaa atgtccaaga gcgatcccga
     2761 tggaagcgtg ctcatccacg acgcgccgga ggacatacgg cgcaagctga gcaaggccta
     2821 ctgccctccc gaggccgagg ggaacccggt gctcgctatc tgcaggtaca taattttcca
     2881 gaggcaggac gtcatgcgca ttgagcgccc ggagaggttc ggcgggaacc tggaggtcaa
     2941 gggctacgac gagcttgaac ggacctacct ggccggaaag ctgcacccca tggacctaaa
     3001 gaacgggacc gccacggcgc tcgcagaggc gctggccccg gccagggagt acttccagag
     3061 gaagcctgag aacctggaga ggatgagagg ggtgctcgca gaggtcaaga agctgcggtg
     3121 acctcatctc agctctctga ggaactccaa ggccttctgc atgcagatgt tcagaatagc
     3181 ctccgcatcg cctatgccga ccagtccggc cgcgccgccg tgcccgcccc cgtcgctgtc
     3241 cgtctcctcc cccactcccc ccagaagctg gccgagatgc agcccctttc gcaccagttc
     3301 ctggcgggcc cgggcgctga ggcggaaccc ctcgttccgc tgggaggcga cgaaggcgat
     3361 gtccgcccct agggacagga gcgccttgca caccgaggac tcaaacgcgc tcccctgcga
     3421 gatggccacg atgtgatcgc ccatccgctc gaagcgcatg cgctgcccgc ccttcatctg
     3481 ggccacgcgc tccgagatgt ccgttgggga atcggtgatc gccaggacct cctccatgct
     3541 cacgtccgcc ccttccatca gacgggcgaa ggcccgaagc gaggctggat tggcgaaatg
     3601 gaagtgcccc gtgtccgtga ggatgcctgc gcagagcccc agggccatct ccctgcttat
     3661 tggccgaccc gcccgctcga tcaggtcaag gacgacctcg gcacagctgc gcttgctatc
     3721 gtcgcagagg tatgtccctc tttccgccca tttgttcgta ggacaatgat ggtcgattat
     3781 tatcatgtcc ccctgcatct gaccatagtc gcctagctgt tcaggcgatg aggtgtcgag
     3841 gatgacgatg agggggtagc tacgctgagg gcgggcgagg agctcgatcc ccaggcgggc
     3901 tgcgacgttc ttggaggtgc ggtccagccc ttcagcgggc aggatgtccg cggggggaaa
     3961 ggccttcgcc agggcatagg cactgcccaa cgcgtcaggg tcggcgttgc catggaccag
     4021 gatgagcttc tccgccgagg agagcttggc tatgatatca tctagcatcc aaggccgcta
     4081 acgtgtcctg gctatattca atttatcgta aaaaaggagc gttagggacc tcaaggctca
     4141 gacgtcctcc gcgtccatcc gcagaggggc gccctgagcg cctatagccc ggctgagctg
     4201 ctcttggacg ttctggaact tctccttgag catcttctcc tggcggtcca ggctcttgat
     4261 gcggacctcc gtggtctctt tgtcctcctc aagttccttc aagagcgcag ggatgtccgc
     4321 taccttgatc atcagggagc caacgttcct gtagaccgtc gcatcctgcg gggtcttctt
     4381 cagctcctcg atggtcatct ccatctcctt gagcatggcg tccatcctta tcttctggcc
     4441 catcaccgtc tgcagctgct gctgcagttg ctggaactgg gcgatctggt tctgaagctt
     4501 ggggctgatc tcatccatct atatcaccga ccgtttcact catcttttcc gctacgcaca
     4561 tccatcggat gtaggaattt aaagctgccc ttagggccgg gaggtcgtcc gcatcaagcc
     4621 tgagggtcac ggacccctgg ggcgcatgaa cctccgcccg ggtgcgtggg atctccctcc
     4681 cggcctccgg accgatggcc ttggccacat tggaggcgaa gggcgagcgc accaccaggg
     4741 tggcccggaa catgtcaccg gccctttatg accttcttga cgttaggccg ctccttatag
     4801 aacagcttgg agccacagct ctcgcactgt ataccgacct ggttgaggtt ggagcgggtg
     4861 gtcttctcgc attgagcgca cttgtagttc atggagtctg ctccttgcca tgggcggcca
     4921 acagctcctt ggagacagcc tccctgatcc tgggcgcata gcaggcggca gcgaacttag
     4981 tctcgcaatg cctgcaggcc cagatcccgg acgctaccct ggtaacgctc atatgatggc
     5041 agctggggca ctcgaagttg gaccgctgct gcttctcgat gtccctggtc agcttcctgg
     5101 tgaccacacc gtacctggtt ccgaacctgc cggagcttcc ggccttcata gttctctttg
     5161 ccattgccat cacccgatca gctttctgat ctccgctccc agtctgcggg aggtatcgat
     5221 cgccgtcctg acctgctcca acgtgagcgc cccgctgagc cccttctgca tggcccgcag
     5281 atcgccgttg tcatcagtgg taacggtcag cctagccgcc gcgaccttct cctcgtcgag
     5341 agtcgggtca accaatatag agttttctat ctgtaccgag gtgacggcca cgggggtgca
     5401 ggtgacgggc atcggaaagt cctcccccag cccattggcc ttggccggga ccgtggcgct
     5461 cttaatggcc gcgaccgctc ccaggttgca ggcgtcgaac aggttgccat cgaaatccag
     5521 gacgtatatg tcaaggaaat tgatccagac cttcttgccc ggctccacgc aaagcctttc
     5581 caggtcgatc atctggctct cccttatccc ccgatcgacc acccgggcca gctcgatcga
     5641 gttctcgtcc ggcggaccgg actcgaagct gggggaggcc atggggatga gctccgcgtt
     5701 cgtcgacagc acacccctgt tgggggtgtc cgggaacggc tctcccacgg tcatcttcaa
     5761 accgacgatg acctcggtat tgcctatctt gaccctcgcc gagccctccg cggtctcgac
     5821 cacgttcgtc tggaccatta tctcccgata ctggtcccac gccctcccat ccacacgcct
     5881 gccgtcggca atgagcctgt ggatatggtc cctcttgatc tctgaaacga cggacctcgc
     5941 catgttcagg cctcctcggt catcgcgtgg acgagcccct tggtctcctc ctcgccctcc
     6001 ccactgacca ccgcatagcg gcggcgcagg gcgtccttct gcagcctgta gatctcggag
     6061 caggccctga cgcccatctc caaacccctg tcgaactcgg cctgggtcat atgcccgtcc
     6121 atctgcatca acagcacctc tccggtccgc gggatgatgg cgatgggaag gtcggcctgt
     6181 ccgaagttgt cctcctcctt gttcaggtcc aggaccacct ggtcattgac cttacccacg
     6241 gcgaccgcgg gcacaacgtc cttcatcggg atgcccgcat cggccagcgc caccgaggcg
     6301 gcggtgaggc cggcgcatct cgtgccggca ttggcctgca gcacctcgat gtacacatcg
     6361 atggacgtcc tggggaagtt ctcggtgaac accacgtact ccatcgcctc ggag
//
LOCUS       DAOSYN010000063         6282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10522_length_6282_cov_4.053798, whole genome shotgun sequence.
ACCESSION   DAOSYN010000063 DAOSYN010000000
VERSION     DAOSYN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10522_length_6282_cov_4.053798"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLJ11_03845"
     CDS             <1..324
                     /locus_tag="PLJ11_03845"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOO03834.1"
                     /translation="KGEYPSAIHYLSAAVGNCLCASLALCLRKARSDPLAIRARVRTV
                     LGRNERGRLRVQGMQVTIFPEVEDPAKLERCLPLFEDFCVVTAAVREGIDVQVSVEPL
                     KNEQR"
     gene            352..792
                     /locus_tag="PLJ11_03850"
     CDS             352..792
                     /locus_tag="PLJ11_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOO03835.1"
                     /translation="MLDDKDRAILSALEKDSRRSTKAIAKDLNIPRATVHERIRRMME
                     RGVIKGFTVVPDFGKLGEPVTAFILVSFLPNNHISQRELAEKIGRLEGVHAVHLISGE
                     HDILLKVRGESMERIGDLVIDKLRQMDGVGRTLTCTCFSTVKDE"
     gene            1069..1422
                     /locus_tag="PLJ11_03855"
     CDS             1069..1422
                     /locus_tag="PLJ11_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03836.1"
                     /translation="MLRSEGEEIEPTLEVRLQDKAPTLPSLVDEGQTRSRSLFGEWKG
                     AVVLCACGSLVHLDEATVLRRRALGKPVECHRCRNRRVALEREDLDNEFYGLDREGDA
                     PGSATPGGQGSYTSP"
     gene            1452..2051
                     /locus_tag="PLJ11_03860"
     CDS             1452..2051
                     /locus_tag="PLJ11_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO03837.1"
                     /translation="MDMVIIAGFLGSGKTTLILNTVDRISERTGKRIAVIVNDFGNIG
                     IDGKVMEKYGLKVQELPRGCICCTLGPNFLETVRMVQDRYDPDLVLVEPSGIADPDSI
                     LATMEHYHGRPLGRITTVVLIDAARFPVLAQAMRVPLERQLHAADMVLMSKADEVDDP
                     RLKEVEAFARNVVGTKPVIPVSNATRSNLDVFVGRLVGQ"
     gene            2048..2461
                     /locus_tag="PLJ11_03865"
     CDS             2048..2461
                     /locus_tag="PLJ11_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03838.1"
                     /translation="MITEDFAAYAARVRLRFPAPVEASDLQAMLESFLGGVAMDCLEA
                     GTRLIGHIKSMVEGGEEVIACSVTDHDARVRCRGRFETPRAEMDVIVNVLQYGLAKRE
                     LARIVATCAARSFPGLETMTIEDLEVEQETLIRLG"
     gene            complement(2445..4889)
                     /locus_tag="PLJ11_03870"
     CDS             complement(2445..4889)
                     /locus_tag="PLJ11_03870"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HOO03839.1"
                     /translation="MKRLVISEKSNAAARIATILSDGGANRRSARGVQVFNFERDGDE
                     CYVVGLRGHIVELDYPPEFNDWSKVDPIDLVKASPAKRVTALNILHTLSELAAECDEV
                     IIATDYDREGELIGLETVTLLERPPRSVRRARFSALTKYEIERAFKELTDPDHRLAES
                     AETRQIIDLAWGAALTRFLSLASGQMGNNFLSVGRVQSPTLTLIVDRHREITEFVPEP
                     YWTVSAKMRKGEDFIGEHAANPFKDEALAEACRTRADKGSGTVVRMERKERDEYPPAP
                     FNTTTMLSEANKLGLSPSMAMKVAEDLYTAGYISYPRTDNTVYPRSLGLRTILEKLRR
                     SDLGKEAEEVLAQETIRPSRGKVETTDHPPIYPTEAATKKELKGAKWTVYELVTRRFL
                     ATLAPPARSETARCELDLGGEPFHADGYRILSPGWRRYYPYWRVNEVLLPDLKAGDQV
                     EVLEVRNERKETRPPSRYSQGSLLQEMERLGLGTKSTRHEIVQKLYDRKYVNGNDLVP
                     TLSGIAVVNALEKHAKIVTESRMTAQLEKDMDAIARGESSLAEVVQESQAMLLDVIRV
                     MSDHRMEIGEEIRSALQEQRFIGTCPKCGKDLRLIRSRKGSEFIGCSGYPECDVTYPK
                     PGGALVQPTDEQCGTCGLPVVKVIRRGSPVRMQCIDPDCASNQGREAVGACPECGKEL
                     RVLYSRAGKRFIGCSGYPECKRTYPLPQFGTLEFLGEACPECGAPVLRVKGKGGWRFC
                     ANMECPTSKKAGSGQGAKAKGEGKKPPAKRSTPKRPVKKADEDVGTEGAGKKASGKAK
                     GPAVKKAAKTVVSPSE"
     gene            complement(4945..5394)
                     /gene="nikR"
                     /locus_tag="PLJ11_03875"
     CDS             complement(4945..5394)
                     /gene="nikR"
                     /locus_tag="PLJ11_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOO03840.1"
                     /translation="MDNVTRIGVSLEPELLEDFDKLIDRKGYITRSEAIRDLIRSALA
                     KEIIKEDENAAVFGTITLFYNHNKSGVKERLMEIQHEHHQHILSSIHVHLDIEQCLEV
                     LIVNGTVKEVKQLSDELGSVKGVIHRTLHRTPVSLGEESAHAHLHEH"
     gene            5460..6245
                     /locus_tag="PLJ11_03880"
     CDS             5460..6245
                     /locus_tag="PLJ11_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOO03841.1"
                     /translation="MGLLALQDDIGNGLQGALTATLRRELTGPTAVLFSGGLDSVLLV
                     ALARQCADMRLYTVGYPGSHDLKAGERAAGELGLPWKGIVLDDDALRAAVIFLRERLG
                     LSDPVVISFELPLYIVCATVPEGVLLSGQGADELFGGYARYAHMTDGERVRSMKEDLR
                     ALLAEGLPREERLATLFGKRLVCPYLSPETIKAAEAVPPTALRGELGNKGPLRDLALG
                     LGLSVARAPKKAAQYGSGTMGAMKRMASAAGMPLGRWAREVNE"
BASE COUNT         1098 a         1945 c         1998 g         1241 t
ORIGIN      
        1 aagggggaat atccaagcgc catccactac ctctctgcgg ctgtgggcaa ctgcctgtgc
       61 gcctcgctgg cgctctgcct gagaaaggcc cgctccgatc cgctggccat cagggcgagg
      121 gtgcgcaccg tcctgggcag gaacgagcgg ggccggctgc gggtgcaggg catgcaggtg
      181 acgatcttcc ccgaggtgga ggacccggcc aagctcgaac ggtgcctccc ccttttcgag
      241 gacttctgcg tggtgaccgc cgcggtccgc gagggcatcg atgtccaagt gtcggtggaa
      301 cccctaaaaa atgagcaaag gtaatctagc ggaagggcgt tcgtctttcc catgctcgat
      361 gacaaggacc gggcgatcct gagcgctctg gagaaggact cccggcgcag caccaaggcc
      421 atcgccaagg acctcaacat cccccgggcc accgtccacg agcgcatccg gcgcatgatg
      481 gagcgcgggg tgatcaaggg cttcaccgtg gtcccggact tcggaaagct gggggagccg
      541 gtcaccgcgt tcatcctggt gtccttcctg ccgaacaacc atatctcgca gagggagctg
      601 gccgagaaga tcggtcgcct ggagggcgtc cacgcggtgc atctcatctc cggggagcac
      661 gacatccttc tcaaggtgag gggcgagtcc atggagcgca tcggcgacct ggtcatagac
      721 aagctgaggc agatggacgg ggtgggacgg acgctcacct gcacctgctt ctccacggtg
      781 aaggatgagt gacccgttaa cggaatcggg ggtaccttca cgtttctgag tagcggtcgc
      841 cctcgttctt atatgaaaat cagcattccc cattatatgc tggatgacat aaaaacctat
      901 ataggacgtt aaggaaatct ttaaaatatc ccaaggcgtt aggcattttt gttcagaata
      961 ttgcgggggt cgagtcttcc ttccagtaac ttgtgggctg ctggttgagg gggcagcatg
     1021 ctctgaatgg gaaggggttt attgcttaac catcaattag cgcaagctat gctcaggtcc
     1081 gaaggcgagg agatcgagcc caccttggag gtgcgcctgc aagacaaggc gccgacgctt
     1141 ccctcgctcg tcgacgaagg gcagacgcgc tcgcgctccc tgttcgggga atggaagggc
     1201 gccgtggtcc tctgcgcctg cgggtccctg gtccatctgg atgaggccac agtgctccgg
     1261 cgccgggcgc ttggaaagcc cgtggaatgc catcggtgcc ggaacaggag ggtggccctg
     1321 gagagagagg acctcgacaa tgagttctat gggctggaca gggaggggga tgctcccggc
     1381 agcgccactc ctggcggcca aggttcttat acatcgccct gaattgattc tgtccagccc
     1441 gaggtaagaa gatggacatg gtcatcatcg ccggcttcct gggcagcggg aagacgacgc
     1501 tcatcctaaa cacagtggac aggatctccg aacggaccgg caagaggatc gcggtcatcg
     1561 tcaacgattt cgggaacata ggcatcgatg gcaaggtcat ggagaaatat ggcctcaagg
     1621 tgcaggagct tccccgcgga tgcatatgct gcacgctggg ccccaatttc ctggagaccg
     1681 tgcgcatggt gcaggacagg tacgacccgg acctcgtcct cgtggagcct tccggcatag
     1741 cggacccgga cagcatattg gccacgatgg agcattacca cggcaggccg ttaggccgca
     1801 tcaccacggt cgtgctcatc gatgccgcgc gcttccccgt gctcgcgcag gcgatgcgcg
     1861 tgcccctcga gaggcagctg cacgcagcgg acatggtgct gatgagcaag gctgacgagg
     1921 tggacgatcc ccgcctgaag gaggtggagg cgttcgcgag gaatgttgtc ggcaccaagc
     1981 cggtgatccc cgtgtccaat gctacgagga gcaatttgga tgtattcgtc ggaagattag
     2041 tggggcaatg ataaccgagg actttgccgc ctacgccgcc cgcgttcgcc tgaggtttcc
     2101 cgccccggtc gaggccagcg atctgcaggc aatgctggag agcttccttg gcggggtggc
     2161 gatggactgc ctggaggcgg ggacccggct gatcgggcat ataaagagca tggtggaggg
     2221 cggcgaggag gtcatcgcct gcagtgtcac cgaccacgat gcccgggtgc gctgccgggg
     2281 taggttcgag acccccaggg cggagatgga cgtgatcgtg aacgtcctgc agtatgggct
     2341 tgcgaagaga gaactcgcgc gcatcgtggc gacctgcgcc gcccgcagct tcccgggcct
     2401 ggaaacgatg accatagagg acctggaggt cgagcaggaa accctcattc gcttgggctg
     2461 accacggtct tggccgcctt cttgacagca ggccccttgg cctttccgga cgccttcttc
     2521 cccgccccct cggtcccaac gtcctcgtcc gccttcttga ccggcctctt cggcgtggac
     2581 ctcttggcgg ggggcttctt accttccccc ttcgctttgg ccccctgtcc ggaccccgcc
     2641 ttcttgcttg tcgggcattc catgttcgcg cagaagcgcc agccgccctt tcccttgacc
     2701 cttaggaccg gagccccgca ctcagggcac gcctccccga ggaactcgag cgtcccgaac
     2761 tggggcaggg gataggtgcg cttgcactcc ggatagccag agcatcctat gaagcgcttc
     2821 cccgccctgg agtagagcac ccggagctcc ttgccgcact cagggcacgc cccgacagcc
     2881 tccctgccct ggttgctcgc gcagtccgga tcgatgcact gcattctgac cggggaaccg
     2941 cgacggataa ccttgacgac cggcaggccg caggtcccgc attgctcgtc cgtcggctgc
     3001 acaagcgctc ctcctggctt gggataggtg acgtcgcatt ccgggtagcc ggagcagccg
     3061 atgaactcgg accccttcct ggaccggatg agcctgaggt ccttgccgca cttggggcag
     3121 gtgccgatga agcgctgctc ctgaagggcg ctccggatct cctccccgat ctccatccta
     3181 tgatcgctca tcaccctgat cacgtccagc agcatcgcct gggattcctg gacaacctcg
     3241 gccagggagg actcccccct ggcgatggcg tccatgtcct tctcgagctg cgcggtcatc
     3301 ctgctctccg tgactatctt cgcgtgcttc tccaaggcgt tgacgaccgc tatcccgctg
     3361 agcgtgggca ccaggtcgtt cccgttcacg tacttgcggt cgtagagctt ctggacgatc
     3421 tcatgcctgg tggacttggt ccccagtccc aggcgctcca tctcctggag gagcgaaccc
     3481 tgggaatatc tcgagggcgg gcgcgtttcc ttccgctcgt tcctgacctc caggacctcc
     3541 acctggtccc cggccttgag atcgggcaga aggacctcgt tcacccgcca atatggatag
     3601 tacctgcgcc agcccgggga caggatgcgg tacccgtccg cgtggaaagg ctcccctccc
     3661 aggtccagct cgcatctcgc cgtttcggag cgggccgggg gggccagcgt cgccaggaaa
     3721 cgcctggtca ccagctcgta gactgtccac tttgccccct taagctcctt cttggtggcc
     3781 gcctccgtcg gatagatggg gggatggtcg gtggtctcca ccttgccccg ggacgggcgg
     3841 atggtctcct gggccaatac ctcctccgcc tccttgccta gatcggacct cctcagcttc
     3901 tcaaggatgg tcctgagccc cagggacctc gggtagaccg tgttgtcagt cctggggtat
     3961 gatatgtacc cggcggtgta taggtcctcc gccaccttca tggccatgga cggcgagagg
     4021 ccgagcttgt tggcctcgct gagcatggtg gtggtgttga agggcgccgg tggatactca
     4081 tccctctcct tccgttccat gcgtacgacc gtgccggagc ccttgtccgc gcgcgtacgg
     4141 caggcctcgg ccagggcctc gtccttgaag gggttggcgg catgctctcc aatgaaatcc
     4201 tcccccttgc gcatcttggc cgaaacggtc caatagggct cgggcacgaa ctcggtgatc
     4261 tccctgtggc ggtccacgat cagggttagg gtagggctct gcaccctccc caccgataga
     4321 aagttgttac ccatctggcc actggcgagg gaaaggaagc gggtgagtgc cgctccccac
     4381 gccagatcga tgatctgcct ggtctcggcc gattcggcca gacggtggtc gggatcggtg
     4441 agttccttga acgccctctc tatctcgtac ttcgtcaagg cgctgaagcg ggcccggcgc
     4501 acggagcgcg ggggcctttc cagcagcgtc accgtttcta ggcctatcag ttccccttcc
     4561 cggtcatagt cggtggcgat gatgacctcg tcgcactccg ccgcaagctc gctgagcgtg
     4621 tgcaggatgt tcagggcggt gacccgcttg gcaggactgg ccttcaccag atcgatgggg
     4681 tcaaccttcg accagtcgtt gaactcaggg gggtagtcga gctcgacgat atggcccctc
     4741 aggcctacga catagcactc gtccccatcc ctttcgaaat tgaacacctg gaccccgcgt
     4801 gcgctccgcc ggttcgcccc tccgtccgag aggatggtgg cgatgcgcgc cgcggcgttg
     4861 gacttctccg agatgaccag tctcttcatc gggagggcat caagaatcaa cagttaaaaa
     4921 ggttatctgc cccatccttg cggatcaatg ctcatggaga tgagcgtgag cggactcctc
     4981 gcccaagctg accggggtgc gatgcagcgt ccggtggatg actcccttga cgctacccag
     5041 ctcatcggac aactgcttga cctccttgac agtgccattg acgatgagga cctccaggca
     5101 ctgctcgatg tccagatgca catggatgga cgagaggatg tgctgatggt gctcatgctg
     5161 gatctccatg agccgctcct tgaccccgct cttgttgtgg ttatagaaga gggtgattgt
     5221 cccgaagacg gccgcgttct catcctcctt gatgatctcc ttggccagcg cgctccggat
     5281 aaggtccctt atggcctcag agcgtgtgat gtagcccttg cggtcgatga gcttgtcgaa
     5341 gtcctccagc agctccggtt ccagggagac gccgatccta gtgacattgt ccatgtgctc
     5401 tgctgtggta ttatcctaat gattattaat gctattcagc gatgaccggg aggaccatca
     5461 tggggcttct cgcgctgcag gacgacattg gaaacgggct acagggagct ttgaccgcca
     5521 ccctgcgacg ggagctgacc ggcccgacgg ccgtcctctt ctccggcggc ctggacagtg
     5581 tccttctcgt cgcgctcgcc cgccagtgcg ccgacatgcg cctgtacacc gtcggctacc
     5641 cgggctccca cgatctgaag gcaggggaga gggccgccgg ggagctcggg cttccctgga
     5701 aggggatcgt tctggacgat gatgcgctcc gggcagcggt gatcttcctc cgagagcgtc
     5761 tgggcctgag cgacccggtc gtcatctcgt tcgagcttcc tctctacatc gtctgcgcga
     5821 ccgttcccga gggcgtcctc ctgagcggcc aaggcgcgga cgagctgttc ggaggatacg
     5881 ctcggtacgc gcatatgacc gatggagaac gggtcagatc tatgaaggag gatctgcgcg
     5941 cgctgctcgc ggagggcctt cctcgagagg agcgcctggc gacgctgttc ggcaagaggc
     6001 tggtctgccc ttacctcagc cctgagacta tcaaggcggc cgaggcggtc ccgccgaccg
     6061 cgctcagggg ggagctggga aataaggggc ccctgcgcga tttggcatta gggctcggac
     6121 tgagcgtggc ccgcgccccc aagaaagcgg cccagtacgg ctcggggacg atgggggcca
     6181 tgaagcgcat ggcctcggcc gcgggaatgc cgctcggtag atgggcccgg gaggtgaatg
     6241 aatgagcacg accctcgagg aggacctgca gtggctctat tc
//
LOCUS       DAOSYN010000064         6266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10551_length_6266_cov_3.788923, whole genome shotgun sequence.
ACCESSION   DAOSYN010000064 DAOSYN010000000
VERSION     DAOSYN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10551_length_6266_cov_3.788923"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4205)
                     /locus_tag="PLJ11_03885"
     CDS             complement(<1..4205)
                     /locus_tag="PLJ11_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOO03842.1"
                     /translation="MATKLEGSGQPIGKQRSPSLSNFSSGIRGSWLGRNWQTVLLLTM
                     MVFLALFVRSYFGYSISVDNGYLVSGGSDSYYHMRVIDHAVATGDHLVWDDMLNYPNG
                     MRNPRPPLYDWSVAVAGMLLSTLTGLAVADAIGLSLIFSTAVWGALTVIPVYMLGKAA
                     FGRKAGLLGAFIFALMAGHIERTVLSNADHDAMVLFFVVFSFYFLLRSLQSVNGTRWV
                     NSWKDPRAIAAGLRAYLGTNQVSLIYAALGGVSVAAVGLIWTGYTYLLVIVLAYLLVQ
                     LLVDRFRNADSMGVLFPIAVFFGLAFLLMAPAYVSLDLVITWFDTPVILYLVGVVGGL
                     MFVVTRDYPWTLVLPVLVAISIVAMVALSFLDPALLDSILSGQGYLVKSKLYSTISEA
                     QAPLFSSLAMSFGMVTFWLALIGVGYAAVKAPKNLNPHFIFVVVWVAISIYMASSAAR
                     FVFNASPAFAVSAGWVLGLIIDRLRFDEMIKGLGTDHGGLWRTIKASVKVRHVLGILF
                     IVLMVLLPNVWFALDAGIPSTLKTDYDAQIYTVMPDLLRPDDYDLENGTYWYLGAFSY
                     GLTLPNEYWPAAWRWFSAQDAEAAVALDRPAFVSWWDYGFEAIQEGAHPAVADNFQNG
                     YNFAGTFLMTQTEEGAIALLIVRCVENDIGGNTAAGAQVLAAMAAHGVDTVRLKDILT
                     HPANYVDEVRGNPEVYGELDDALSAANAKYVAAREVLSRLDKDQLISIYNEVRQITGN
                     DIGYVAVDSRLFPFTATSYNIFYAPAKLSDQRVDGNNIPYDYYNIYAVDYYGNEIPLD
                     EVTSDDYIVSYSIEYTDLFYDTMLYRAFMGYGPNDVGESEQGLPGISGSLQSVPSMQG
                     WNMSNFRLVYRTAYYNPYPSSDVANHSDAWRAVSYMEAVEMYNKISAGELIGTVDLSS
                     SGLYSGVTFLQYYDGAIVEGTAVTDAGRPMAGIWVTVLDEYGIPHQVVRTDADGGYSV
                     LAPFGDVTIVYSYGDLDPRLLYGTELHTESLHITYDQAMRVPADVDKDDQLDYLIDLD
                     VMIEAGSLAGSVYLDLDGNGKYSATSDEMLVGATVIFENATNGFRAETTSTATGYELP
                     GISPMNGTIWAEYDGHAFGGTGVQVKVGTPVALDLHVEPAKVSGNATLEDGTAADQVT
                     ISLLDRSNGEVLEAVTDALGAFSFEGLLPGNYTIQTPDGTVAVNADMTLEEGDALSLD
                     LVLYGSMRLSGLVSYDGQAVANALVGLSGELGDVWVSSDSRGRYSVVMPKGDVSLYAT
                     ATVGGAEAVRLLKVTATESAVVDLSLEGGLVLDGTVLSSGAAAVRAEVRLQDIASGAV
                     LPSLTNSKGVFRAVLPQGVYSVYISDGTRAYWGDVGLDASKAITFNLLPGVKLSGTVW
                     YDADGNGAASASEAQGDVVLTVT"
     gene            complement(4324..4974)
                     /gene="pyrF"
                     /locus_tag="PLJ11_03890"
     CDS             complement(4324..4974)
                     /gene="pyrF"
                     /locus_tag="PLJ11_03890"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOO03843.1"
                     /translation="MRRESRVILALDEVRPERAFMVAERTADVVDAIKINWPLVLATS
                     PETITRLAGLAPVICDFKVADIPNTVSLIVSQAMGRGAEGVIVHGFAGSDSVRAAVEA
                     AGSGKIFVVTEMSHPGGAEYTAPVAGSLARMAVRCGAAGIIAPATRPERLAALRREVG
                     PLLILSPGVGAQGGSASEALRMGADYVIVGRGIYGAEDPREAALRVVREAKAVPLP"
     gene            5055..6113
                     /locus_tag="PLJ11_03895"
     CDS             5055..6113
                     /locus_tag="PLJ11_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03844.1"
                     /translation="MRPIPMESSSWYTKRLPRGCRLCRQGAKMVLLVTGRCGESCYYC
                     PLSEAKKGRDVVYANELRVASDEDIVREAEAMGARGTGITGGDPLLVMDRTVRYVRLL
                     KERFGTAHHIHLYTATVDRERFLRLQEAGLDELRVHPPIRHWGRLGDLGLTEAVEGLD
                     MKVGFEVPVVPGEGEGLIALCRFAAANGLAFVNLNELEVSETNCQALLARGLHVRSDV
                     SSAIEGSERTAMAVMEEVGDAVPVHFCSSSFKDRVQLRERLKRRAKRVARPLDLVTSE
                     GMVLLGVVETQDLEGAYRLLRDAHGVPAELMAIERGRLEVAPWVLEALAPGLPFPCFL
                     VEEYPTADRLEVERRPLG"
BASE COUNT         1080 a         2021 c         2136 g         1029 t
ORIGIN      
        1 tcggtgacgg tcagcacgac atcgccctgc gcctccgagg cggaggccgc cccgttgccg
       61 tccgcgtcgt accatacggt gccgctgagc ttgacccccg gcaggaggtt gaaggtgatg
      121 gccttggatg cgtctaggcc aacatcgccc cagtaggccc tcgtcccgtc gctgatgtag
      181 acggagtaca cgccttgggg caggaccgcc cggaacacgc ccttggaatt ggtgagcgag
      241 ggaagcacgg ccccgctggc gatgtcctgc agacggacct ctgccctcac cgcggcagcc
      301 ccggaagaga ggacggtgcc atcgaggacc agccccccct ccagggacag gtccacgacc
      361 gcgctctcgg tggcggtgac cttcaacagg cgcacggcct ccgcgccccc gacggtggcc
      421 gtggcgtaca gggacacgtc gcccttgggc atcaccacgg aatagcgccc gcgggagtcg
      481 ctcgataccc agacatcgcc cagttcgccg gacagcccga ccagggcgtt ggccaccgcc
      541 tggccgtcgt agcttaccag cccgctcagg cgcatcgagc cgtagagcac caggtccagg
      601 gagagggcgt cgccctcctc gagggtcatg tcggcgttta cggcgacggt gccgtccggc
      661 gtctggatgg tgtagttccc ggggaggagc ccctcgaagc tgaacgcgcc gagcgcatcg
      721 gtcacggcct cgagcacctc cccgttggag cgatccaaga gggatatcgt gacctggtcg
      781 gcggcggtgc cgtcctccag ggtcgcgttc ccgctgacct tggccggctc cacatggagg
      841 tccagggcca cgggcgtgcc caccttgacc tgcaccccgg tccccccgaa ggcgtggccg
      901 tcgtactcgg cccatatcgt gccgttcatg gggctaatgc ccggcagctc gtacccagta
      961 gctgtggacg tcgtctcggc ccggaacccg ttggtcgcgt tctcgaagat gactgtggcc
     1021 ccgaccagca tctcgtcgct ggtcgcggag tacttgccgt tcccgtcgag gtccaggtag
     1081 accgatccgg ccagggagcc ggcctcgatc atgacgtcca ggtcgatgag gtagtcgagc
     1141 tggtcatcct tgtccacgtc cgccggcacg cgcatggcct ggtcataggt gatgtgcagg
     1201 gactcggtgt gcagctcggt cccgtagagc aggcgggggt ccaggtcgcc gtaggagtag
     1261 acgatggtga cgtccccgaa cggcgccagc acgctgtagc ctccgtcggc gtcggtgcgc
     1321 accacctgat gggggatccc gtactcgtcc aggacggtga cccagatccc tgccatcggc
     1381 cttccggcgt ccgtgaccgc ggtgccctcg acgatggccc cgtcgtagta ctgcaggaag
     1441 gtgaccccgg agtacagccc cgaggaggac aggtccactg tgccgatcag ctcgcctgcg
     1501 cttatcttat tgtacatctc caccgcctcc atgtaggaga ccgccctcca ggcatcggag
     1561 tggttggcca catcgctgct ggggtagggg ttgtagtagg cggtgcggta caccaggcgg
     1621 aagttgctca tgttccagcc ctgcatggac gggacgctct gcaggctgcc ggagatgccg
     1681 ggcagcccct gctcgctctc ccccacgtcg ttggggccgt agcccatgaa ggcccggtag
     1741 agcatggtgt cgtagaacag gtcggtgtac tcgatggagt agctgacgat atagtcgtca
     1801 gaggtgacct cgtccagcgg gatctcgttc ccgtagtagt cgaccgcgta gatgttgtaa
     1861 tagtcatacg ggatgttgtt gccatccacc cgctggtccg acagcttggc cggcgcatag
     1921 aagatgttgt aggaggtggc ggtgaagggg aagaggcggc tgtccaccgc gacgtagccg
     1981 atgtcgttcc ccgtgatctg ccggacctcg ttgtagatgg agatcagctg gtccttgtcc
     2041 agacgagaga gtacctcccg ggccgccacg tacttggcgt tggcggcact gagggcgtcg
     2101 tccagctccc cgtacacctc ggggttgccc ctcacctcat ccacgtagtt cgccggatgg
     2161 gtcaggatgt ccttgagccg gaccgtgtcc accccatggg cggccatcgc ggccagcacc
     2221 tgggcgccag cggcggtgtt gccgcctatg tcgttctcca cgcagcgcac tatgagcagg
     2281 gcgatggccc cctcctcggt ctgggtcatg aggaacgtcc cggcgaagtt gtagccgttc
     2341 tggaagttgt cggcgaccgc cggatgggca ccctcctgga tggcctcgaa gccgtagtcc
     2401 caccaggaga cgaacgcggg acggtcgagg gcgacggccg cttcggcgtc ctgggcggag
     2461 aaccatctcc acgcggccgg ccagtactcg ttcggaaggg tgagcccata gctgaacgcc
     2521 cctaaatacc agtaggtgcc gttctccagg tcgtagtcgt ccggcctcag gaggtccggc
     2581 atgaccgtgt agatctgcgc gtcatagtcg gtcttcaggg tcgaggggat ccctgcgtcc
     2641 agggcgaacc acacgttggg cagcagcacc atcagcacga tgaagaggat accgagcacg
     2701 tgccttacct tgaccgaggc cttgatggtc ctccatagcc caccgtggtc cgtgcccagg
     2761 cccttgatca tctcgtcgaa ccgcaatcgg tcgattatga gcccgagcac ccagcccgcg
     2821 gagacggcga aggccgggga ggcgttgaac acgaacctgg cggccgagga ggccatgtag
     2881 atcgagatcg ccacccagac caccacgaat atgaaatggg ggttgaggtt cttcggggcc
     2941 ttgaccgcgg cgtagcccac cccgatgagc gccagccaga acgtcaccat gccgaaggac
     3001 atggcgaggc tggagaacag cggggcctgc gcctcggaga tggtgctgta cagcttgctc
     3061 ttgaccaggt acccctggcc ggacaggatg gaatcgagca gggcggggtc gaggaacgag
     3121 agcgctacca tggccacgat ggagatcgcg acgaggacgg ggaggaccag ggtccaggga
     3181 tagtcgcggg tgaccacgaa catgaggccg ccgacgaccc cgaccaggta caggatcacc
     3241 ggggtgtcga accaggtgat gaccaggtcc aggctcacat aggcgggtgc catcagcaga
     3301 aaggccaggc cgaagaagac ggcgatgggg aagagcacgc ccatggagtc cgcgttgcgg
     3361 aagcggtcca ccagaagctg gaccagaagg taggcgagca cgatgaccag gaggtaggtg
     3421 tacccggtcc agatgaggcc caccgctgcc acgctcaccc ctcccagcgc cgcgtagatg
     3481 agcgagacct gattggtccc caggtacgcc ctgagcccgg ccgctatggc cctagggtcc
     3541 ttccagctat tcacccatct ggtcccgttg acggactgca gggaccgaag caggaagtag
     3601 aaggagaata ccacgaagaa cagcaccatt gcgtcgtggt cagcgttcga gagcaccgtc
     3661 cgctcgatgt gccccgccat tagggcgaag atgaacgccc cgaggaggcc ggccttgcgg
     3721 ccgaaggccg ccttgccgag catgtacact gggatgacgg tcagggcccc ccagaccgcg
     3781 gtggagaaga tcagggacag gcctatggca tcggcgaccg ccagcccggt cagggtgctg
     3841 aggagcatgc ccgccaccgc caccgaccag tcgtacagcg gggggcgggg gttcctcatc
     3901 ccattcggat agttcagcat gtcgtcccac accaggtggt ccccggtcgc caccgcgtgg
     3961 tcgatcaccc tcatgtgata gtaggaatcg gagccgcccg agaccaggta gccgttgtcc
     4021 accgagatgg agtacccgaa gtagctcctg acgaacaatg ccaggaacac catcatcgtc
     4081 aataacagca ccgtttgcca attccttccg agccaggagc ccctgatgcc gctggagaaa
     4141 ttggacaacg aaggagagcg ctgcttgcca atgggttgac cggacccctc tagtttcgtt
     4201 gccattaagt gagtctcccc tggggtgatt accaactgat gacggacccc gtataaatac
     4261 gttggtccca tccaggtcaa taaaatccga tgcctggcag cccgccggga gcgatggttc
     4321 ggctcaggga agagggacgg ccttggcctc ccgcacgacc cgcagggccg cctcccgggg
     4381 gtcctccgcc ccgtagatgc cgcggccgac gatgacgtag tccgcgccca tgcggagggc
     4441 ttccgaagcg ctccccccct gggcgcccac gcccggggac aggatgagga gggggccgac
     4501 ctcccgccgc agcgccgcca gccgctccgg ccgtgtggcc ggggcgatga tgcccgcggc
     4561 gccgcagcgc acggccatgc gggcgagcga tccagcgacc ggcgcggtgt actccgcccc
     4621 cccggggtgc gacatctcgg tgacgacgaa gatcttcccg ctcccggcgg cctcgaccgc
     4681 cgccctgacc gagtcgctgc ccgcgaagcc gtggacgatg accccctccg ccccccgccc
     4741 catcgcctgg gagacgatca gggagacggt gttggggatg tcggccacct tgaagtcgca
     4801 gatgaccggg gcaagcccag ccagcctggt gatggtctcc ggggaggtgg ccaggaccag
     4861 aggccaattg atcttgatgg cgtccacgac gtccgccgtc cgctcggcca ccatgaaggc
     4921 ccgttccggc ctgacctcgt ccagggcgag gatgaccctg ctctcccttc gcatttcgcg
     4981 aatcccaacg gcccgccccg atatagggtt tgccataggg aagcgttaag tccgccccgg
     5041 tccgtagagg gggcatgcgc cccatcccca tggaatcgtc gtcctggtac accaagcgcc
     5101 tcccgcgcgg ctgcaggctc tgccgacagg gggcgaagat ggtcctcctg gtcaccggca
     5161 ggtgcggcga gtcgtgctac tactgccccc tatccgaggc caagaagggg agggacgtgg
     5221 tatatgccaa cgagctccgg gtggcgagcg atgaggacat cgtccgggag gccgaggcca
     5281 tgggtgccag ggggaccggc atcaccggcg gcgacccgct gctggtcatg gaccggacgg
     5341 tgcgctacgt ccggctgctc aaggagcggt tcggaacggc gcatcacatc cacctgtaca
     5401 cggccacggt ggaccgggag cggttcctcc gcctgcagga ggcggggctg gacgagcttc
     5461 gggtgcaccc gccgatcagg cactggggcc gcctcgggga cctcggcctg accgaggcgg
     5521 tggaggggct ggatatgaag gtggggttcg aggtgccggt ggtccccggc gagggggagg
     5581 ggctaatagc gctctgccgg ttcgcggccg ccaatggcct ggccttcgtg aacctgaacg
     5641 agctggaggt gtccgagacc aactgccagg ccttgcttgc ccggggcctc cacgtccgct
     5701 cggacgtgtc cagcgccatc gagggaagcg agcggacggc catggcggta atggaggagg
     5761 tgggggacgc ggtgcctgtc cacttctgct cctccagctt caaggacagg gtgcagctgc
     5821 gcgagcggct gaagcggagg gcgaagcggg tcgcaaggcc cttggacctg gtgacctccg
     5881 agggcatggt cctgctgggg gtggtggaga cgcaggatct cgagggcgcc taccggctcc
     5941 tcagggacgc gcatggcgtc ccggccgagc tgatggcgat cgaaagggga aggctggagg
     6001 tggccccttg ggtcctggag gccctggccc ccgggcttcc gttcccctgc ttcctggtgg
     6061 aggagtaccc caccgcggac aggctggagg tggagaggcg gccccttggc taggccaccg
     6121 agatgctctg cacgttgtga tggcggttga ccaggtcccg ggcgatcttc aggttcttcc
     6181 cgttcttgcc gatggcccgg cccttcacgg ccgggtcgac cgtgacggtg gcgtggacga
     6241 tgttccccct cgtctcgatc tccacc
//
LOCUS       DAOSYN010000065         6236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10615_length_6236_cov_4.226986, whole genome shotgun sequence.
ACCESSION   DAOSYN010000065 DAOSYN010000000
VERSION     DAOSYN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10615_length_6236_cov_4.226986"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLJ11_03900"
     CDS             complement(<1..284)
                     /locus_tag="PLJ11_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03845.1"
                     /translation="MEKFAQDLEALMAKLVVGCDAPCEEALCKLEQRLLDLKEKNLLK
                     INHSVLEMIVAKYLVLDGYEVDLEHMIDAGLSCDVYARKGEGVLIVEVETG"
     gene            complement(320..559)
                     /locus_tag="PLJ11_03905"
     CDS             complement(320..559)
                     /locus_tag="PLJ11_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOO03846.1"
                     /translation="MNSAIVAITNDIGTEGKVMEALCQMPAVRECYMVYGVYDILAKV
                     QASSEEEMSAAIHSIRDLKGVRSTLTLVVCEEHKK"
     gene            complement(633..782)
                     /locus_tag="PLJ11_03910"
     CDS             complement(633..782)
                     /locus_tag="PLJ11_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03847.1"
                     /translation="MPLDEAKDCYVGVPYSLCVAHEITSEGYGFSVSCETGTITDVFQ
                     PSLEE"
     gene            complement(1336..1857)
                     /locus_tag="PLJ11_03915"
     CDS             complement(1336..1857)
                     /locus_tag="PLJ11_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HOO03848.1"
                     /translation="MHVVFEGADTEDYESGPMEYVGYQEGRFDQDIIEFFVAGEPVPQ
                     GSTKAYYIKKLERVVTTHTNANTENWRMRIATEAQRTNELRSVSYFSDDRRLGYEVAM
                     DFVFTRPKSQPKKWRMNTKRPDLDKLVRAALDAITGVLIPDDSQVVRIAAGKSYGDGD
                     RGPGLYISVRRVE"
     gene            complement(1919..3223)
                     /gene="aspS"
                     /locus_tag="PLJ11_03920"
     CDS             complement(1919..3223)
                     /gene="aspS"
                     /locus_tag="PLJ11_03920"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOO03849.1"
                     /translation="MLYDSRSITANEYDKEVTVEGWAQEVRNLGGISFLILRDRYGTV
                     QVTAPKKRTAPDVMQTIASLARESVVRVTGTVKASPQVRAGYEIVPASIQVVGPAKAP
                     LPMGVVDKVDVEPETRFNHRYMDLRRPEVRAVFELKSLTTRLIDEYLVDNGFVEVFTP
                     KLVASGAEGGSTLFKVDYFGRPAYLAQSPQLYKQMLMATGLDRVFEIGPAFRAEPSDT
                     ARHVSEFISFDGEMAHIDSQADVMAMVEGCTQFVIAEVRERGARELSVLGVDVSVPKA
                     PYPVIPYERAIAMVREAGLRIELGDDLGTEGERLLGDLMLQDGHEMYWIIEYPEAAKP
                     FYIMEKDGTPYSFSFDLDYRGQEISSGGQREHRYDRLVQRMQRLGLDPGSFEFYLNAF
                     AYGMPAHGGWGLGVERMVQKMLGLSNVRETILFPRDRNRLVP"
     gene            3355..3579
                     /locus_tag="PLJ11_03925"
     CDS             3355..3579
                     /locus_tag="PLJ11_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOO03850.1"
                     /translation="MPVKITIDQNECTGCGICYNDECPDVFMEGDDGNSNVKPMYQKG
                     DKAVGEVPDSLKGCVKNAEASCPASAISVQ"
     gene            3672..4820
                     /locus_tag="PLJ11_03930"
     CDS             3672..4820
                     /locus_tag="PLJ11_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HOO03851.1"
                     /translation="MSMSQKVTVSVIKADVGSVGGHSKPHPKMMRKCEEVLAEGVRAG
                     VINDYYVTRVGDDINLFMTHYKGENHREVHSLAWSAFQEAAKLAKEMKMYAAGQDILS
                     DAFSGNIRGMGPGAAEIEFTERGAEPMVFFMADKTEPSSYSMPLARIFMDPFTTTGLV
                     IDPRAHEGFRFEIVDVMDSKRIVMSAPEETYDILSLIGDTTRYAIKKVLSKDKNIGPA
                     CVVSTEKLNICAGKYVGKDDPVAVVRCQSGLPAVGEVLQPFMFPALVAGWMRGSHYGA
                     WYPCSVEDSDPTYFDGPPRICALGFQISNGRFQGCEDPDSPLGKKVPVDYFASSVWDE
                     ARRKAVRASVYMRQHGPFMPSILGPEEMEYTTRPAVLSKLKDRMEKID"
     gene            4813..5493
                     /gene="tpiA"
                     /locus_tag="PLJ11_03935"
     CDS             4813..5493
                     /gene="tpiA"
                     /locus_tag="PLJ11_03935"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOO03852.1"
                     /translation="MIELRAPVIIVNFKGYPEVEGAKATALASICEDVARRTGAAIIV
                     CPPMVELSKVASSVAIPVFAQHVDAKAPGSITGYVSPQSVKAAGAKGTLLNHAEHRLP
                     FPEIASLVDTCRGLGLISVVCADTAESAATIASVSPDYIAVEPPELIGGDVSVTTADP
                     QVVRRTVELVKGVDPRVRVLCGAGVKGGRDVRAALALGAEGVLLASGVVKAKDARAAL
                     LDLVPSEG"
     gene            5556..>6236
                     /locus_tag="PLJ11_03940"
     CDS             5556..>6236
                     /locus_tag="PLJ11_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOO03853.1"
                     /translation="MAILLTLLLSLPALPSLAEDAPDIPSLVISRACPAYPGEFITIT
                     NIGEAVDLAGWRISDGEGSVTFAAPLPLQGMASVTWGGADARFPELYPGEAWLPSGSP
                     GLRSEGNLKLADAGDQVLLMDPSGRTVDVLVYGNAEPLPSWSGPPVACKKGLMLLRSA
                     DGTGPGCWEARTPGVYSVSTPHWYAEVEPLLYPEDGLPALVREVDLARESVELACYII
                     ENWTLARHS"
BASE COUNT         1106 a         2060 c         1907 g         1163 t
ORIGIN      
        1 ccggtctcca cctccacgat gagcacgccc tcccctttcc gggcgtacac atcgcagctg
       61 aggccggcat cgatcatgtg ctccaggtcc acctcatagc cgtccagcac gaggtacttg
      121 gccactatca tctccaggac ggaatggttg atcttgagca ggttcttctc cttgaggtcc
      181 agcaatcgtt gctccagctt gcaaagggcc tcctcgcacg gtgcatcgca cccgacgacc
      241 agcttggcca tcagcgcttc caggtcctgg gcgaacttct ccaaaaggat gcctctgctc
      301 ggtaatcgcg acctatccct tactttttat gctcctcgca caccaccagg gtgagggtgg
      361 acctcacccc cttgagatcg cggatggaat ggatggcggc gctcatctcc tcctccgagg
      421 aggcctggac cttggccagt atgtcgtaga cgccatacac catatagcat tcccgcacag
      481 ccggcatctg gcacagggct tccatcacct tcccttcggt gccgatgtca ttggttatgg
      541 ccacgatggc gctgttcatg gtctatgata tgaactgccc ttaatagaaa cctaccgtct
      601 ggtgaagaag gtggacggca ggggcatggc gctcactcct ccagcgaggg ctggaagacg
      661 tcagtgatgg tcccggtctc gcaggacaca ctgaagccgt acccttcgga ggtgatctca
      721 tgggccacgc acagggagta cgggaccccg acatagcagt ccttcgcttc atccaagggc
      781 atttggttca ttctatccct cttttttttg gaattgcaca ttatcatcgg atggaagata
      841 acgatgctgt tccgcggtct gcatcttccg tgtatatgtg tccgttctcg gacatcgtag
      901 gagcgatcga ggctcggcaa tggccgatcc tccgatcgcc gccctccatg ctttgcagga
      961 cagcgcggtc ctgacgacct cgatcgacga tgtcccagcc ctctaagccc cgggcggccc
     1021 gtggatggga acggccctgg cgtggtccgg tccttcttga ccagcattcc gccataggcc
     1081 caggccattt cccggaaggg gcatgcgcac gaggctccga acagtccgat gtccataagc
     1141 cccatctttt gctcgatacg ggtcgacgag atcgaccggg acccacggac atccagcctg
     1201 caaacgttta tctcaccgga gagggtaagg gggttcagtc attggaccgt tccccggatc
     1261 gatgaagaga agagggtcag ctgatccccg cggacatgat gaaccctatg agtgcagaga
     1321 tcccggtcca ggacctcatt ccacccttct gacggagatg tagaggccgg gaccgcggtc
     1381 gccgtcgcca taggacttgc ctgcggcgat gcggaccacc tgcgaatcgt ctggaattag
     1441 aacgccagtg atcgcgtcca gggccgcgcg caccagcttg tcgaggtccg gccgcttggt
     1501 gttcatccgc cacttcttcg gctggctctt cggtctggtg aacacgaagt ccatcgccac
     1561 ctcgtacccg aggcgccggt cgtcggagaa gtagctgacc gaacgcagtt cgttggtccg
     1621 ctgggcctcg gtcgcgatgc gcatcctcca gttctcggtg ttggcgtttg tgtgggtggt
     1681 tacgaccctt tccagcttct tgatgtagta ggccttggtg gagccctgcg gcaccggctc
     1741 gccggcgacg aagaactcga tgatgtcctg atcgaaccgc ccttcctgat accccacata
     1801 ctccatgggt ccggactcgt agtcctcggt gtccgcccct tcgaatacca cgtgcatccc
     1861 aggggggata tgaatcccgt cactaatata atttatccgc tggaagcgaa ctgcgctctc
     1921 agggcaccag ccggttgcgg tcccttggga acaggatcgt ctcgcgcacg ttgctcagcc
     1981 cgagcatctt ctggaccatc cgctccaccc ccagccccca gccgccgtgc gccggcatgc
     2041 cgtaggcgaa ggcgttcagg tagaactcga acgagcccgg gtccaggccc agccgctgca
     2101 tcctctgcac caggcggtcg tagcggtgct ccctctgccc cccggacgag atctcctgcc
     2161 ccctgtagtc caggtcgaag gagaacgagt agggggtgcc atccttctcc atgatgtaga
     2221 acggcttggc cgcctcgggg tactcgatga tccaatacat ctcatggccg tcctggagca
     2281 tcaggtcccc gagcagcctc tcgccctcgg tcccgaggtc atcgcccagc tcgatcctga
     2341 gccccgcctc ccttaccatg gcgatcgccc tctcgtacgg gatcacgggg tagggcgcct
     2401 tggggacgga gacgtcgacg ccgaggacgc tcagctccct ggccccccgc tccctcacct
     2461 ccgcgatgac gaactgcgtg cacccctcca ccatggccat gacgtcggcc tgggagtcta
     2521 tgtgcgccat ctccccgtcg aagcttatga actccgagac gtggcgggcg gtgtcggacg
     2581 gctccgccct gaacgccggc ccgatctcga acacccggtc caggccggtg gccatgagca
     2641 tctgcttgta cagctggggg gactgcgcca ggtaggcggg cctgccgaag tagtccacct
     2701 tgaacagcgt ggagccgccc tcggccccgg aggccaccag cttgggtgtg aacacctcga
     2761 cgaacccgtt gtccaccagg tactcgtcga tgaggcgcgt cgtcagggac ttgagctcga
     2821 agaccgccct gacctcgggc ctcctcaggt ccatgtacct atggttgaac cgcgtctccg
     2881 gttccacgtc caccttgtcc acgacgccca tgggcagcgg ggccttggcc gggcccacga
     2941 cctggatgga ggcggggacg atctcgtacc cggccctcac ctgggggctc gccttgaccg
     3001 tcccggtcac gcgcaccacg gactcgcgcg ccagggaggc gatggtctgc atcacgtccg
     3061 gggcggtccg cttcttggga gcggtgacct ggaccgttcc atagcggtcc cggaggatca
     3121 ggaaggatat ccctccaagg ttgcgcacct cctgcgccca accctcgacg gtgacctcct
     3181 tgtcgtattc gttggcggtg atggagcggg aatcgtagag catgcgcacc cctcgttcgg
     3241 aacgccccca tatcaccgat ttcacttata agcgttggcc gtccagccat ctatcgcccc
     3301 ctccggccct ccgaagcgaa aggttaaagg tcctcagaaa acgttctctc cctcatgcct
     3361 gttaagatca ccatagacca gaacgagtgc accggatgcg ggatctgcta caacgacgaa
     3421 tgcccggacg tcttcatgga gggggacgac ggcaactcca acgtgaagcc gatgtaccag
     3481 aagggggaca aggccgtcgg ggaggtcccc gacagcctga aggggtgcgt gaagaacgca
     3541 gaggcctcct gccccgcgtc ggcgataagc gtccaataga ccaagggagc ccccgcgctc
     3601 tctcggcatt cagcgatatc agctcatcaa ccattatata tcgttgcagt aattctgagc
     3661 caaggtatgg gatgtccatg tctcagaagg tcactgtctc tgtaataaag gccgatgtcg
     3721 gttcggtggg cggccattca aagccgcacc cgaagatgat gcgcaagtgc gaggaggtcc
     3781 tcgccgaggg cgtgcgggcc ggggtcatca acgactacta cgtcaccagg gtcggggacg
     3841 acatcaacct gttcatgacc cattacaagg gcgagaacca cagggaggtg cactcccttg
     3901 cctggtccgc cttccaggag gccgccaagc tggccaagga gatgaagatg tacgccgcgg
     3961 gccaggacat attgtccgac gccttcagcg ggaacatccg tggtatgggg cccggggcgg
     4021 ccgagatcga gttcacggag cggggcgccg agcccatggt cttcttcatg gccgacaaga
     4081 ccgagccctc ctcctactcc atgccgctgg cgcgcatctt catggacccg ttcaccacca
     4141 ccgggcttgt catcgacccg agggcccatg aggggttccg cttcgagatc gtggatgtca
     4201 tggactccaa gaggatcgtg atgtccgccc ccgaggagac ctatgacatc ctgagcctga
     4261 taggcgatac caccagatac gccatcaaga aggtcctcag caaggacaag aacatcggcc
     4321 ccgcgtgcgt ggtcagcacg gagaagctga acatctgcgc cgggaagtac gtcggcaagg
     4381 acgaccccgt ggcggtggtg cggtgccaga gcggcctgcc tgccgtgggc gaggtcctgc
     4441 agccgttcat gttccccgcg ctcgtggccg ggtggatgcg cggctcccac tacggcgcct
     4501 ggtacccctg ctcggtggag gactccgacc ccacctattt cgacggcccc ccgcggatct
     4561 gcgcgctggg cttccagatc agcaacggcc gcttccaggg ctgcgaggac cctgactccc
     4621 cgttgggcaa gaaggtcccg gtggactact tcgccagcag cgtttgggac gaggccagga
     4681 ggaaggcggt ccgcgcgagc gtgtacatgc gccagcacgg gccgttcatg ccctccatcc
     4741 tcggccccga ggagatggag tacaccaccc ggccagcggt cctgagcaaa ttgaaggaca
     4801 ggatggagaa gattgattga gcttcgcgcc cctgtgatca tcgtcaattt caagggctac
     4861 cccgaggtgg agggggccaa ggcgaccgcc ctcgccagca tatgcgagga cgtggcccgt
     4921 aggacgggcg cggccatcat cgtgtgcccg cccatggtcg agctctccaa ggtggcctcc
     4981 tcggtcgcca tccccgtctt cgcgcagcac gtcgacgcca aggcacccgg ctccatcacc
     5041 gggtacgtct ccccgcagag cgtcaaggcc gcgggagcga aggggacgct gctcaaccat
     5101 gccgagcatc gcctcccgtt cccggagatc gcctcactgg tcgatacatg ccgaggccta
     5161 gggctgataa gcgtggtttg cgctgacacg gcggaatcgg ccgcgacgat cgcatctgtc
     5221 tcccccgact acatcgccgt ggagccgccc gagctcatcg ggggggacgt ctcggtcacc
     5281 accgccgatc cccaggtcgt ccgcaggacc gtcgagctgg tcaaaggggt cgatcccagg
     5341 gtcagagtgc tgtgcggcgc gggcgtgaag ggcggacggg acgtgagggc ggccctcgcc
     5401 ctgggggccg agggcgtcct gctcgcctcc ggcgtggtca aggccaagga cgcaagggcg
     5461 gcgctgctgg acctcgtccc ctccgaaggc tgattgcatc cccaaccccc atttcggccc
     5521 cctccatttt ttgcaccgtg cgtaaacttc ccgcggtggc gatcctcctc acgctcctat
     5581 tgtcgcttcc ggcgctcccc tccttggcgg aggatgcgcc tgacatcccc tcactggtaa
     5641 tttccagggc gtgccccgcc tatcccgggg agttcatcac gatcaccaac atcggggagg
     5701 ccgtggacct cgcgggatgg cgcatcagcg atggggaggg cagcgtaacg ttcgccgccc
     5761 cgctcccatt gcagggcatg gcaagcgtga cctggggtgg ggccgacgcc cggttcccgg
     5821 agctgtaccc cggggaggca tggctcccct caggctcccc cggcctgcgc tccgagggga
     5881 acctcaagct cgccgacgcc ggggaccagg tccttctgat ggacccctcc ggcaggacgg
     5941 tggacgtgct ggtctacggg aacgcggagc cgcttccttc ctggagcggc ccgcccgtcg
     6001 cctgtaagaa ggggctcatg ctcctcagga gcgccgacgg gaccggtccc ggatgctggg
     6061 aggccaggac ccctggcgtc tattcggtga gcacgcccca ttggtacgcc gaggtcgagc
     6121 ccctgctgta cccggaggac ggcctgcccg ccctggtgag ggaggttgac ctagcccgcg
     6181 agagcgtcga gctcgcctgc tacatcatag agaactggac cttggcaagg cattcg
//
LOCUS       DAOSYN010000066         6213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10654_length_6213_cov_4.609776, whole genome shotgun sequence.
ACCESSION   DAOSYN010000066 DAOSYN010000000
VERSION     DAOSYN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10654_length_6213_cov_4.609776"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLJ11_03945"
     CDS             <1..580
                     /locus_tag="PLJ11_03945"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOO03854.1"
                     /translation="WHLRTAGALIMAAAFIVLIGIITAEALYPGYHTGERTISALVEE
                     EPSGAIFTIAMVVGGTVLSLASLLLPGAGLPTSLTVPALLMGVGCVGVGLFPMQTGAP
                     HVIAAALALGMGGIAALMAARAARPPFSYISLVLGAFGLASLLLFAIMGRTGPLGPLG
                     EGGVERWAAYADLAWAMGFGGHLMGKGAAPSR"
     gene            662..1051
                     /locus_tag="PLJ11_03950"
     CDS             662..1051
                     /locus_tag="PLJ11_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03855.1"
                     /translation="MEPDQAIHLCRSTRRDLRRLMSALERRSFYDIGHEIGQVAATGA
                     AFRNMDEELRWLEQLLTVGNYETIRENAWTVYDLVNYYDRMIDLIKSRPIPPGRATPL
                     VDSIQAWRQNAGRLAQMVCQIEDGEVP"
     gene            1086..1505
                     /locus_tag="PLJ11_03955"
     CDS             1086..1505
                     /locus_tag="PLJ11_03955"
                     /inference="COORDINATES: protein motif:HMM:NF018621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HOO03856.1"
                     /translation="MEERMQEVRLRHHHLLCTMTFSGHGYDRGFVANMEEVVARLRSP
                     DGQWVRLEASCDALCAACPNAAEGMCRDHRSVLRKDRSAALFLGLPERAVLEAAPLIR
                     SVEERMRSLTDIRQVCGDCQWAELCNEWLRGRRSLEG"
     gene            1550..3322
                     /locus_tag="PLJ11_03960"
     CDS             1550..3322
                     /locus_tag="PLJ11_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO03857.1"
                     /translation="MDAELRIILDMTLLMVIAGLCSLIFARIKMPPILGYLTAGIILG
                     PTMFPELWVEQTTVFVLSNIGIVMLMFWIGLEQSASKLRRVGSRLVLIVTMEMTLVVI
                     IGYLGGMAMGLGSTASIFLGAIISGTSTAVVVGVLQTNKTVTKEQATAIVSITVFEDV
                     GQVIILSMAAPLLAGDSPALGSTVNMVIGLVIFFGLTIMLGLAVIPRLMDRIGERYPS
                     EIVFIVAVGMAFTMALLSSSIGLSIAIGPFLMGLIVSTSLYSERIKAKVVPVKELFMA
                     VFFISIGLQMDPMLIVYNLPLVMAIAVLFIVGKVLAVGISTYIFGFRAKDSFVVAISL
                     PAMGEFAFIIAKIALDAGLLSQELYSSVIGAALITMVTLPLSSKLQEPAYRALVRAIP
                     KRTRAALGRIDSVKNAVMAKNGMRTKERNEVGRHLTRIFIDFLLIVLILMIFNFVNEL
                     EEAVIGVTGEAGLLSSAVLLFLMLLAISPPVVSIYWHLKKLSMTLTDMATRSQRIAEG
                     SGRNLYRMIIDLNAIVMFLLVLALIFPFMPPAVVRSPWMFMGILLAVVVVAVLAWDAL
                     KNSYVRLVGMVSGAGRSEVEEKKE"
     gene            complement(3341..3715)
                     /locus_tag="PLJ11_03965"
     CDS             complement(3341..3715)
                     /locus_tag="PLJ11_03965"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HOO03858.1"
                     /translation="MPRPPPDLESRPGKAILSWKAKIATGIKMGVYVVMSKLTREGRK
                     TLKNRPERLKEVNKEISKMGAKVVEQYALLGKYDFLNILEAPDNETVAKVMVALGSRG
                     TLETATMAAIPIDDFLRALEEE"
     gene            complement(3696..4934)
                     /locus_tag="PLJ11_03970"
     CDS             complement(3696..4934)
                     /locus_tag="PLJ11_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO03859.1"
                     /translation="MSGASRSEGKAGVKVPGHVKWLILLTSFSSIGYGYLTTVISAYL
                     PEVGVSSGQVGLIIGVSGLAMIISAIPFGLMSDRIGRKRLLLVGLAALPLSMFICSLS
                     TSPDVFMLAGIISGVAEGAILSTWNAMIADKTTVDNREMAFSISFIINGAFSGLGLAM
                     PFAFPFLEDLGGWTSGQVHSWAFVLLGFVTAISPITVNRLLRDHEDKVPSGGLRRGSF
                     LKERSKRTLLRFSINNTLIGLGAGLIIPLISTWFYLRYGIPDSFSGPLLALSSLSIAV
                     AAIFSPRLAQRLGQVKAIVLTQGLSTLFMVAIPFVPGAALAGAVYLVRSALMNMAGPI
                     MDSYLMGIVDKDDRGLASAINSIVWRVPNSATTIIGGVLLASGYYALPFVLASGIYIT
                     AVIAFWFNFRDVKVYAEAPA"
     gene            5056..5457
                     /locus_tag="PLJ11_03975"
     CDS             5056..5457
                     /locus_tag="PLJ11_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03860.1"
                     /translation="MPEISAAHRTADYGASFDREDGHGEGGAVEAGQGIHPPFIWEVV
                     DLARTWETVPHGNGDDIPFKAIAGRRACTERRRPLGGVPEAVPRLRAGMPRPWRAGGP
                     EVLRAGDEIPVQGGAGVDTGKVQGPRSPDGP"
BASE COUNT         1092 a         1938 c         2022 g         1161 t
ORIGIN      
        1 ctggcacctc agaaccgctg gggccttgat catggcggcg gcgttcatcg ttctgatcgg
       61 catcataacc gcggaggcgc tctaccccgg gtaccacacg ggcgaacgga ccatcagcgc
      121 tctggtggag gaggagccct ccggcgccat cttcaccatt gcgatggtgg tgggcgggac
      181 ggtcctttcc ctggcctccc tgctccttcc aggtgctggc cttcccacgt cccttaccgt
      241 ccccgccctc ctgatggggg tgggctgcgt gggcgtcggg ctgttcccta tgcagaccgg
      301 ggcgccgcac gtcatcgccg ccgcgctggc cttgggcatg gggggcatag cggccctcat
      361 ggcggcgcgg gccgcacgcc cgccgttcag ctatatctcc ttggtcctgg gagccttcgg
      421 gcttgcctcc ctcctcctct tcgccatcat ggggcggacc ggtccgctcg gcccgctggg
      481 ggagggcggg gttgagcgat gggccgccta tgcggacctg gcgtgggcga tggggttcgg
      541 cggccacctg atggggaagg gagcggcccc atccagatga tagggccagg gaactggccg
      601 tggcggggcg gcgaaaggta gattaccctt gaccctctag ttatcaatag agagggatca
      661 tatggagccg gaccaagcga tccacctctg tcgttcgacg agaagggacc tgaggaggct
      721 catgtccgcc ctggaaagga ggagcttcta tgatatcggg catgagatcg gacaggtcgc
      781 agcgaccggc gccgccttcc ggaacatgga cgaggagctg aggtggttgg agcagctgct
      841 caccgtaggg aactacgaga ccatcaggga gaacgcatgg acggtgtatg atctggtgaa
      901 ctactacgac cggatgatcg atctcattaa gagtaggccc atcccccctg ggagggcaac
      961 gccgctggtg gattccatcc aggcctggcg gcagaacgcg gggagattgg cccagatggt
     1021 atgccagatc gaggacgggg aggttccctg agcccagaag ggccattcaa ggaggggcgt
     1081 tccccatgga ggaacgaatg caggaggtcc ggctcagaca tcatcatctt ctgtgcacca
     1141 tgacgttcag cggacatggt tacgaccgtg ggttcgtggc caacatggag gaggtggtgg
     1201 cccgcctgcg gtcgcctgac ggacaatggg tccgcctcga ggcctcctgc gatgccctat
     1261 gcgccgcgtg ccccaatgcc gcggagggga tgtgcaggga ccaccgttcg gtccttcgga
     1321 aggaccgctc cgcggcgctg ttcctcggcc tgccggagag ggcggtcctg gaggccgctc
     1381 cgctcatcag gagcgtggag gagcggatgc gctcgctgac ggacatccgc caggtctgcg
     1441 gggactgcca atgggcggaa ctgtgcaatg aatggctccg ggggcgccgg agcttggaag
     1501 gataggccgg ggcgataacc ttttagtagc attccatttt ctaggggaca tggacgccga
     1561 gctgaggatc atcttggaca tgacccttct gatggtcatc gccggcctct gctcgctgat
     1621 cttcgcccgc atcaagatgc cccccatcct gggctacctg acggccggca tcatcctggg
     1681 ccccaccatg ttccctgagc tatgggtgga gcagaccacg gtgttcgtgc tctccaacat
     1741 aggcatcgtg atgcttatgt tctggatcgg gctggaacag agcgcatcca agctgaggag
     1801 ggtcggctcc cgtctcgtcc tcatcgtgac catggaaatg acgctggtcg tgataatagg
     1861 ctacctgggg gggatggcca tggggctcgg cagcaccgcc tccatcttcc tgggcgcgat
     1921 catatctggt accagcacgg cggtggtggt cggtgtgctc cagaccaata agaccgtcac
     1981 gaaggagcag gccaccgcca tcgtgagcat aacggtgttc gaggatgtgg ggcaggtcat
     2041 catcctttca atggccgcac ccctgctcgc gggcgactcc cccgccctgg gctcgaccgt
     2101 caacatggtc atcggcctgg tcatcttctt cggcctcacc atcatgctcg ggttggcggt
     2161 catcccgagg ctgatggacc gcatcgggga gcgctacccc tcggagatcg tgttcatcgt
     2221 cgccgtgggg atggcgttca ccatggccct cctctcctcc tccatcgggc tgtccatcgc
     2281 catagggccg ttcctcatgg gcctcatcgt ctccacgtcc ctgtacagcg agcgcataaa
     2341 ggccaaggtg gtgccggtga aggagctctt catggccgtc ttcttcatat ccataggcct
     2401 gcagatggac ccgatgctga tcgtatacaa ccttcccctg gtcatggcca tcgccgtcct
     2461 gttcatcgtc ggcaaagtgc tggccgtggg gatcagcacc tacatcttcg ggttcagggc
     2521 caaggacagc ttcgtggtgg ccatctccct tcccgccatg ggcgagttcg ccttcatcat
     2581 cgccaagatc gctttggacg ccgggctttt gagccaggag ctgtactcct cggtcatcgg
     2641 ggccgccctc atcaccatgg tcaccctgcc gctgtcctcc aagctccagg agccggccta
     2701 cagggcattg gtccgggcga taccgaaaag gacaagggcg gccttggggc gcatagactc
     2761 ggtcaagaac gcggtcatgg ccaagaacgg gatgcggacc aaggagagga atgaggtcgg
     2821 gaggcacctc acccgcatct tcatcgattt cctgctgata gtcctcatcc tgatgatctt
     2881 caacttcgtg aacgagctgg aggaggcggt catcggtgtg accggtgagg ccggcctcct
     2941 ttcgagcgcg gtactgctct tcctcatgct gttggccatc tcccctccgg tggtcagcat
     3001 ctactggcac ctgaagaagc tgtccatgac cctcactgac atggccacgc gctcccagag
     3061 gatcgccgag gggagcgggc ggaacctcta ccgcatgatc atcgacctca acgcgatcgt
     3121 catgttcctg ctggtccttg ccctgatctt cccgttcatg ccccctgcgg tggtgaggag
     3181 cccctggatg ttcatgggca tcctgctggc cgtggtcgtg gtggcggtct tggcatggga
     3241 tgccctgaag aactcctatg tgcggctggt gggaatggtc agtggagcgg gccgctcaga
     3301 ggtcgaggag aaaaaggaat aagggggcgg cgacccccgt tcactcctct tcgagcgccc
     3361 tgaggaaatc gtcgatgggg atcgcggcca tcgtagccgt ctccagggtc cctcgggacc
     3421 caagggcgac catgaccttg gccaccgtct cgttgtccgg cgcctccagg atgttcagga
     3481 agtcgtactt cccgaggagc gcgtactgct cgaccacctt tgcccccatc ttggatatct
     3541 ccttgttgac ctccttcaac cgctctggcc tgttcttgag ggtcttgcgg ccctcccggg
     3601 tcagctttga catcactacg tatacgccca tttttatccc cgttgctatc ttggccttcc
     3661 aggacaatat agctttcccc ggccggctct ccaggtcagg cgggggcctc ggcatacacc
     3721 ttcacatccc ggaagttgaa ccagaaggct atgaccgccg tgatgtagat ccctgatgcc
     3781 agcacgaagg ggagggcgta gtatcctgag gccaggagca cgcccccgat gatcgtggtc
     3841 gcgctgttcg gcaccctcca cacgatggag ttgatcgcgc tggccagtcc cctatcgtcc
     3901 ttgtccacga tgcccatgag ataggagtcc atgatggggc cggccatgtt catcagcgcc
     3961 gagcggacca ggtagaccgc tcccgccaag gcagccccgg gcacgaacgg gatggccacc
     4021 atgaagaggg tggataggcc ttgggtgagg acgatcgcct tcacctgtcc taagcgctgg
     4081 gccagacggg ggctgaatat ggccgccacg gctatggaga gggaggacag ggccagcagg
     4141 ggcccggaga aggagtcggg gatgccgtag cgcaggtaga accaggtgga tatcagcggg
     4201 atgatgagcc ccgccccgag gccgatgagg gtgttgttga tcgagaagcg caacagggtc
     4261 ctcttgctcc gctccttaag gaagctgccg cggcgaaggc ctccggacgg taccttgtcc
     4321 tcatggtccc tcagcaatcg gttgacggtg atcggggaga tggcggtcac gaatcccagc
     4381 aggacgaagg cccaggagtg gacctgtccg gatgtccatc cccccaggtc ctccaggaac
     4441 ggaaaggcga acggcatggc caggcccagg ccgctgaagg ccccgttgat gatgaaggag
     4501 atggagaagg ccatctcgcg gttgtccacg gtggtcttgt cggcgatcat ggcgttccat
     4561 gtggagagga tggccccctc ggccacccca gagattatcc ctgccagcat gaagacgtcg
     4621 ggggatgtgg agagcgagca gatgaacatg gagaggggca gcgcggccag gccgacgagg
     4681 agcaaccgct tgcgcccgat gcggtcggac atcagcccga aggggatggc gctgattatc
     4741 atggccagac cggacacccc gattatgagc ccgacctgcc ccgagctcac gcccacctcg
     4801 ggcaggtagg cggatatgac cgtggtcagg tacccgtacc caatggagct gaaggaggtg
     4861 aggaggatga gccatttgac atgaccgggg accttcaccc cggccttccc ttcggacctg
     4921 gacgcgccgc tcatcctccc tcctcccttc cccgacaccc attgaaccgg tttggtcggc
     4981 gaatcgggtt ctactatcag atacttgcgg tcggaaggcc tctggttagg gttccattga
     5041 ctcgctgcga tacccatgcc cgagatctct gccgcccatc gcaccgctga ctacggggca
     5101 tcgtttgatc gagaggacgg ccacggggag ggcggagcgg tggaggcggg gcaggggatc
     5161 catccgcctt tcatctggga ggtggtcgat ctcgcgcgga cctgggagac cgttccccac
     5221 ggcaacgggg atgacatccc tttcaaggcc attgcaggga ggagggcctg cacggagcgc
     5281 cgccggcccc tggggggcgt tcccgaggcc gttccaaggc tgcgcgccgg gatgccgcgc
     5341 ccatggcggg caggaggacc ggaggtcctt cgtgccgggg atgagatccc tgtccaaggc
     5401 ggagcagggg ttgatacagg aaaggttcaa ggacctcggt ccccggatgg cccgtgagcg
     5461 caatgcttgg gcggtggcgc gcccatgcag aggacgatcg gtgcgcccct tacccgaggg
     5521 gggatcgctg acgccgtcgg gtgcggggat gcgcccatgc cccgggccac ggggacctga
     5581 ggacgggcgt cccagccatt atggccgttg taggcctccc agacggatag gcgtgccctg
     5641 tgtgcgggga ggaatgggcg ccctcgagcc cctgcgaagg gcgaatgcct tgaggagggg
     5701 gactgtgcga tcgacgatgg acatatcgta gacaagcaga cgcaaatgat taatcgccct
     5761 cccgccattc ccctttgatg ctctgtccaa ggtgcggcag ccaccctctg gccactgaca
     5821 atttctgcat ggagtgcggg accccgaagg cccagttcgt cgacgaggag cgcccggaga
     5881 ggccggcccc atccccgcag ttcgtccctc agcccgaggc gccgcagggc ccacctgccc
     5941 catcgtcggt gagaccaatg cccccacagg ggcctcccta tcgagccccg gtccaacccc
     6001 agcccccagt tcagatcggc cagcagggcc ctcagcccct gccccaggcg atcccttaca
     6061 aaggctcatc cgagggatgg ccctcgagcg ggtatggcca gatgggtcca taccaggtgt
     6121 acctcccacc gaaggccccg ccgttccagc cccagtcccc acccttgtcc tcccctacgc
     6181 ctcagcagtc ctcggctcca tcggccacca aac
//
LOCUS       DAOSYN010000067         6143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10798_length_6143_cov_3.791393, whole genome shotgun sequence.
ACCESSION   DAOSYN010000067 DAOSYN010000000
VERSION     DAOSYN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10798_length_6143_cov_3.791393"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLJ11_03980"
     CDS             <1..547
                     /locus_tag="PLJ11_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03861.1"
                     /translation="PEGGEEERIVPLCLSLQEVVGLSAKAIGGFSHRLELVPHYLFSY
                     EVPLYVGEGRIGSKEGIVAVNALTKEVQGWPEVPGTVPRLEQGHKRLEPVLEEEEAFS
                     LVRAHVEREHTSERDMVRENGQVTMTERVTVHPRLDLGLVRPLGLYYVPIWCVEGTKG
                     VMVLDASTGRVLSEDHFQDLC"
     gene            complement(516..992)
                     /locus_tag="PLJ11_03985"
     CDS             complement(516..992)
                     /locus_tag="PLJ11_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOO03862.1"
                     /translation="MAEPRFLLDGMLGSLARWLRIMGYDALYVRDASDADMLARLVGN
                     DRTLLTRDRQLADRAGGRGLYLEEGELEGQLLTVVERFGLSTEGRMARCSACNGELEA
                     VGKEAVAGLVEAGTLSGHAEFWRCRDCGKIFWQGAHWRTIRDRLQRVSRGPGSGPR"
     gene            complement(985..1620)
                     /locus_tag="PLJ11_03990"
     CDS             complement(985..1620)
                     /locus_tag="PLJ11_03990"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HOO03863.1"
                     /translation="MRPKVIVNCAMSPDGKIAGTERRQLRISSPEDMERVRRLRSECQ
                     AILVGAGTIVADDPHLTVKGLPPERQPLRVILDPRGKVPETAMVLDARARTLVVTVRE
                     CERIYPGAETVRLGTGRIDLEALLALLGARGVSTLLVEGGGETIYSFFRAGLVDVYSV
                     FIGDFIIGGREAPTPVDGEGFRTGEHIPLRLVSVERLGAGVHLTYEVLRNG"
     gene            1697..2473
                     /locus_tag="PLJ11_03995"
     CDS             1697..2473
                     /locus_tag="PLJ11_03995"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOO03864.1"
                     /translation="MNDFDIIELRRSNLRGATVIEGFSSIGLVGSITANYLVNLLDLD
                     LIAVIDSPYLPSVSIVRDGVPYSPVRVYAGMIGEKGTDKIVVIVSEFEPPQEILRPLA
                     WTIMDWVEAKGCRMVISPEGLANRAGDEAEEPEEETTEFKVYAVASTPRARNLLVKNN
                     IPIFENGVVVGLAGVLLNEGANRNFDVVSILSEAHSDYPDARAAAAATEAIDKLLLHT
                     RLDTQPLLEEAGLIEEALKEIKKKTGESEELAKKRSIMYG"
     gene            complement(2460..3467)
                     /gene="rtcA"
                     /locus_tag="PLJ11_04000"
     CDS             complement(2460..3467)
                     /gene="rtcA"
                     /locus_tag="PLJ11_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HOO03865.1"
                     /translation="MLELDGSQGEGGGQTVRTALSLSVITGTPFHITEVRAGRPEPGL
                     KPQHLAGVQLMQRLSSAEVEGASIGSRELTFRPGTLTGGRVEFDVGTAGSLTLLVQTV
                     LPALVTSSKPTELVLTGGTDVRWSPPVDHYRMVLLPLLRRMGANVELEVVRRGFYPKG
                     GGLVRLRVEGGPLSPMRLEERGGLVRVRGRACVQNLPEKVADRMLASAAAEVLGMTRE
                     REVAVGPSPGAVLTLAAEYEGTVLGASALGERGVPAERVGREAAACLRQAMEGGGTVD
                     ARTADQLVPFMALADGPSCFTVGRISGHLASQMGLLPQFLPVAFNVEGGPPYRVELTR
                     T"
     gene            complement(3461..>6143)
                     /locus_tag="PLJ11_04005"
     CDS             complement(3461..>6143)
                     /locus_tag="PLJ11_04005"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOO03866.1"
                     /translation="GTRTEGLSTAEARERLVRYGPNELGEGRGPSPLRLFLGQFKDLM
                     VIVLIIAALISAGIGAWKGSAEEWLDAGIILIIVMVNAVLGFVQNYRAERTMQALKEM
                     AAPKATVVRDGEERSVPSRDLVPGAIIILTTGDRISADARLLEAVNLKVNEAPLTGES
                     APVRKSAEAVLPPDAPLAERSNMVFSGCTVDHGRGRAVVTATGMTTQLGTIAQMVRVE
                     SESTPLQRKLDRLGRQLGLIVLGVCVFIFAVGYLRGVPVEEMFLTSVSLAVAAIPEGL
                     PAVVTISLALGLQRMAKRKAVMRRLPAVEALGSATVICTDKTGTLTRGEMNVREVYVG
                     ETISVSGEGFEPEGEFLVNGGPIAPAEREDLISLLMAGALCNDSSLYQEGGRWKVRGD
                     PTEGALVVAAMKAGLDVEGLARRSPRLYEVPFDSTKKRMTTVHEMDGRRIAFMKGAAE
                     SVVPLCTRYRTREGEHPLDKATADAILAKDNEMAGRALRVLAIATRDVSDVPLEEAAI
                     EREMTFLGLVGMMDAPRSEAVEAVRRCHAAGIRVIMITGDHELTARAIALELGIAGPG
                     RDRVVSGKALEAASPEELAEMLRTTNVFARVAPEHKMRIVEALQAQGEIVAMTGDGVN
                     DAPALKRADIGVAMGVTGTDVSKEASQMVLMDDNFATIVNAVEEGRGVYDNIRKFVAF
                     LLSCNSGEVIAMFVASLVLVGPEYMPFLLPVHLLWMNLVTDGLPALALGVEPIEKGVM
                     QRPPTDPNEPPLTRRAAVRVLIIGGVIALSALLAFQLADLLALSSGQADNVRHARTVA
                     FCTMVLAQLFLGLSARSETLTIRRLGLFTNRKMVLAIAVSVALQLAVVYVPVLSGAFR
                     TAPLGLAEWALILPLSMLALAVNEAWKVLASRREGRC"
BASE COUNT         1013 a         2232 c         1945 g          953 t
ORIGIN      
        1 cccggaaggc ggcgaggagg agcggatcgt cccgttgtgc ctgtcccttc aggaggtcgt
       61 ggggctctcc gccaaggcca taggggggtt cagccaccga ctggagctgg tgccccatta
      121 cctcttcagc tacgaggtcc ccctctacgt cggggagggg cgcataggca gcaaggaggg
      181 catagtggcg gtgaacgccc tcaccaagga ggtgcagggc tggcccgagg tgccgggaac
      241 ggtgccccgc ctcgagcaag ggcacaagcg gctggagccc gtactggagg aggaagaggc
      301 cttctccctc gtcagggcgc acgtggagag ggagcacacc tccgagcggg acatggtgag
      361 ggagaacggg caggtcacga tgaccgagcg ggttacggtg cacccccggc tggacctggg
      421 cctggtaagg cccctcggcc tgtactacgt ccccatatgg tgcgtggagg gcaccaaggg
      481 agtgatggtc ctcgacgcca gcaccggcag ggtgctcagc gaggaccact tccaggacct
      541 ctgctgaccc gctgcaggcg gtcccttatc gtcctccagt gcgctccctg ccagaagatc
      601 ttcccgcagt cccggcatcg ccagaactcg gcatggccgg agagcgtccc ggcctccaca
      661 aggcccgcga cggcctcctt cccgacggcc tccagctccc cgttgcaggc cgagcagcgg
      721 gccatcctcc cctccgtgga aagcccgaac cgctcgacga cggtcaggag ctgcccctcc
      781 agctccccct cctcaaggta cagccccctc cccccggccc ggtcggccag ctgtctgtcc
      841 cgggtgagca gcgtcctatc gttccccacc aggcgcgcca gcatgtccgc gtccgaggcg
      901 tccctcacgt acagggcatc gtagcccatg atcctcagcc accgggccag ggaccccagc
      961 atcccgtcca ggaggaagcg cggctcagcc attccgaagg acctcgtagg tgaggtgcac
     1021 gccggcgccc aggcgctcga cggagaccag ccgcaacggg atgtgctctc ccgtccggaa
     1081 cccctccccg tccaccgggg tcggcgcctc ccgccccccg atgatgaagt cccctatgaa
     1141 gaccgagtag acatcgacga gcccggcccg gaagaacgaa tagatcgttt cccccccgcc
     1201 ctccaccagg agggtggaga cgcctcgggc cccgagaagg gcgagcagcg cctccaggtc
     1261 tatcctcccc gttcccaggc ggaccgtctc cgcccccgga tagatgcgct cgcactcccg
     1321 gacggtgacc acaagggtgc gcgcccgggc gtccaggacc atggccgtct cgggcacctt
     1381 gccccggggg tcgaggatca ctcggagggg ctggcgctcc ggcgggagcc ccttgacggt
     1441 caggtggggg tcgtccgcga cgatggtgcc ggcgccgaca aggatggcct ggcactccga
     1501 gcgcaggcgc ctcacccgct ccatgtcctc gggggaggag atgcggagct gcctccgctc
     1561 cgtcccggcg atcttcccgt cgggggacat ggcgcagttc acgatgacct tgggcctcat
     1621 ggaccggata tcatcaatga tcaccataag ggtgatggga aggttgatta atagcgccac
     1681 accgatatcc aaggcgatga acgatttcga cataatcgag ctgcgcaggt ccaacctccg
     1741 gggcgctacc gtaatcgaag ggttctccag catagggctg gtcggttcca ttaccgcgaa
     1801 ctacctggtc aacctgctgg acctggacct gatcgcggtg atcgactccc cttacctccc
     1861 gtccgtgtcc atcgtccgcg atggcgtccc ctattccccg gtgcgcgtct atgcggggat
     1921 gataggggag aaggggaccg acaagatcgt ggtcatcgtc tccgagttcg agccccccca
     1981 ggagatcctc aggcccctgg cctggaccat catggactgg gtggaggcca agggttgcag
     2041 gatggtcatc tcgcccgagg gcctcgcgaa cagggcgggg gacgaggccg aggagccaga
     2101 ggaggagacg acggagttca aggtgtacgc cgtggcctcc acccctcggg cacggaatct
     2161 gctggtgaag aacaacatcc ccatcttcga gaacggcgtg gtagtgggcc tcgccggcgt
     2221 gctgctcaac gagggggcca atcgcaactt cgatgtcgtc tccatcctct cggaggccca
     2281 ttccgactat ccggacgccc gggccgccgc ggcggccacc gaggcgatcg acaagctgct
     2341 gctccatacc cgcctggaca cccagccctt actggaggag gccggcctca tcgaggaggc
     2401 cctcaaggag atcaagaaga agacagggga gagcgaggag ctggccaaga agcgttccat
     2461 catgtacggg tgagttccac cctgtacggc ggccccccct ccacgttgaa ggccacaggc
     2521 aggaactggg ggagcaagcc catctgggag gccaggtgcc cgctgatccg ccccacggtg
     2581 aagcatgagg ggccgtccgc caaggccatg aacggcacca gctgatcggc cgtgcgcgca
     2641 tcgaccgtac ccccgccctc catggcctgc cgcaggcagg ccgcggcctc ccgtccgacc
     2701 ctctccgccg ggaccccccg ctcgcccaag gcgctcgctc ccaggaccgt tccttcgtac
     2761 tcggcggcca gggtaagcac cgccccgggg gacggcccga cggccacctc ccgctccctc
     2821 gtcatcccga ggacctccgc cgcggcggag gcgagcatgc ggtccgccac cttctccggc
     2881 aggttctgca cgcaggccct gccccggacc cttacgaggc cgccccgctc ctcgagcctc
     2941 atcggggaca gcgggccgcc ctcgaccctg aggcgcacca gccctccccc cttggggtag
     3001 aacccccttc tcaccacctc cagctccacg ttggcgccca tgcgcctcag gagggggagg
     3061 agcaccatcc ggtaatggtc gacgggcggc gaccagcgca catcggtgcc gccggtgagc
     3121 acgagctcgg tgggcttcga cgaggtcacc agggcaggaa gcacggtctg gaccagcaat
     3181 gtcaggctgc cggccgtccc cacgtcgaac tcgacccttc ccccggtcaa ggttccaggg
     3241 cggaaggtga gctccctgga gccgatgctg gccccctcca cctccgccga ggagaggcgc
     3301 tgcatgagct gaaccccggc cagatgctgc ggcttgagcc ccggctccgg ccttcctgcc
     3361 cggacctcgg tgatatggaa gggggtgccg gtgatcacgg acagcgagag cgctgtcctc
     3421 accgtctgtc cccctccctc cccctgggaa ccgtccagtt ctagcatcgg ccctccctcc
     3481 tagaggccag caccttccag gcctcgttga ccgccagcgc cagcatgctg agaggaagga
     3541 tcagcgccca ttccgccagc ccgagcggcg cggtgcggaa ggccccgctc agcaccggca
     3601 catagaccac cgccagctgg agcgccaccg acaccgcgat ggccaggacc atctttcggt
     3661 tcgtgaacag gccgagccgc cttatcgtaa gcgtctcgga cctggccgag aggccgagga
     3721 agagctgcgc cagcaccatc gtgcagaagg cgacggtccg ggcgtgccgc acattgtccg
     3781 cctggccaga ggagagggca aggaggtcgg cgagctggaa ggccaaaagg gccgagaggg
     3841 cgatcacccc gccaatgatc aggacgcgga ccgccgcccg ccgcgtgagc ggcggctcgt
     3901 tcgggtcggt gggcggccgt tgcatgacgc ccttctctat aggctccact cccagggcca
     3961 gggcggggag cccgtccgtc acaaggttca tccacagcag gtgcacgggc agcaggaacg
     4021 gcatgtactc cgggcctacc aggaccaggc tggccacgaa catggcgatg acctcgccgg
     4081 agttgcagga gaggaggaag gccacgaact tcctgatgtt gtcatacacc ccccttccct
     4141 cctccaccgc attgactatg gtggcgaagt tgtcgtccat gagcaccatc tgggatgcct
     4201 ccttggagac gtcggtcccg gtcacgccca tggccacccc gatgtccgcc cgcttcagcg
     4261 ccggcgcgtc gttcaccccg tcgccggtca tcgccacgat ctccccctgg gcctgcaggg
     4321 cctcgactat gcgcatcttg tgctccgggg ccaccctggc gaacacgttg gtggtcctca
     4381 gcatctccgc aagctcctcg ggcgaagctg cctcgagcgc cttgccgctg acgacccggt
     4441 cccggcccgg gccggctatc cccaactcca aggcgatggc cctggccgtg agttcgtggt
     4501 cccccgttat cattatcacc cggatgccgg cggcatggca ccgccgcacc gcctccacgg
     4561 cctcgctccg cggggcgtcc atcatgccca ccaggcccag gaaggtcatc tcccgttcta
     4621 tcgccgcttc ctccaagggc acgtccgaga cgtccctggt cgctatggcc agcacccgca
     4681 gcgcccgccc ggccatctca ttgtccttgg ccaggatggc gtccgccgtg gccttgtcca
     4741 ggggatgctc cccctccctc gtccgatacc tggtgcagag ggggaccacg ctctccgccg
     4801 cccccttcat gaaggcgatc ctcctcccat ccatctcgtg cacggtggtc atgcgcttct
     4861 tcgtcgaatc gaaggggacc tcatagaggc gggggctcct cctggccaac ccctccacgt
     4921 ccaggccggc cttcatggcg gcgaccacca gcgcgccctc tgtggggtcg ccgcgcacct
     4981 tccacctccc tccctcctga tacaaggagg agtcgttgca tagggcgccg gccatcaaga
     5041 gcgagatcag gtcctcccgc tcggcagggg cgatcggtcc gccattcaca agaaactccc
     5101 cctcaggctc gaacccttcc ccgctcacgc ttatggtctc cccgacgtag acctcccgga
     5161 cgttcatctc tcccctggtc aacgtcccgg tcttgtccgt gcagatgacg gtggcgctgc
     5221 ccaacgcctc gacggcgggg agccggcgca tcaccgcctt gcgcttggcc atgcgctgca
     5281 ggccgagggc cagggagatc gtgacgaccg ctggcagccc ctcaggaatg gccgcgacgg
     5341 ccaggctgac ggatgtcagg aacatctcct ccaccgggac cccgcgcagg tagccgacgg
     5401 cgaagatgaa cacgcatacg ccgagcacga tcagtcccag ctgcctgccc agccggtcca
     5461 gcttccgctg gagaggggtg ctctcgcttt ccacccgcac catctgggcg atggtgccga
     5521 gctgggtagt catccccgtg gcggtgacca cggcccttcc ccgcccatgg tccacggtgc
     5581 atccggagaa caccatgttg ctccgctcgg ccagcggcgc atcggggggg agcaccgcct
     5641 ctgcgctctt gcggaccggg gcagactccc cggtgagggg ggcctcgttg accttgaggt
     5701 tgaccgcctc cagcagccgg gcgtccgcgg agatcctgtc ccccgtcgtc aggatgatta
     5761 tggcccccgg cacaaggtcc cgcgagggca cgctccgctc ctccccgtcc cggaccacgg
     5821 tcgccttggg ggctgccatc tccttgaggg cctgcatggt cctctcagcc ctgtagttct
     5881 gcacaaagcc caggaccgcg ttgaccatca cgatgatcaa gatgatgccg gcgtccaacc
     5941 actcctcggc ggagcccttc caggccccga tgccggcgga gatcagcgcg gcgatgatga
     6001 ggacgatgac catgaggtcc ttgaactggc ccaagaacag ccggaggggg gagggcccgc
     6061 gcccctcgcc aagctcgttg gggccgtagc gcaccagccg ctcccgggct tccgcggtcg
     6121 aaagcccctc ggtcctcgtg ccc
//
LOCUS       DAOSYN010000068         6114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10871_length_6114_cov_4.702756, whole genome shotgun sequence.
ACCESSION   DAOSYN010000068 DAOSYN010000000
VERSION     DAOSYN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_6114_cov_4.702756"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1592
                     /locus_tag="PLJ11_04010"
     CDS             195..1592
                     /locus_tag="PLJ11_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOO03867.1"
                     /translation="MKEYDLIVIGSGAGLNVVAKARQRKMKVALAENGPMGGTCLNRG
                     CIPSKLLATPAELVRTIADAKRIGIHATVEKADFQFVRERMWKLIAPDRDGILESIRR
                     DSGIDLFQTTVHFVGRNTLQAGDEQITSKRIVIAAGARTKVPNVPGLAEAGFLTTENF
                     FELSELPESMLILGGGYKAYELGMFLASFGCRTTIIGRNPRLLKGEEPEISAIVLKRA
                     REYMDVLVDQEVLAVRREGSRKVAIVRDRSSGAGREVGAEQILVATGVQGNADWLRPE
                     AAGIAVDEGNYVLVDRYLETNVPGIFAIGDIIGRTMFRHTANYHSELVWHNMFSEKRA
                     ELDEHAVPHAVFGYPEVGSVGLTEEECKRRGHTILVGYSEYMDCAKGYAMGEEDGLVK
                     VIVDKGSHRILGAHIVGAHAALLVQQVVYLMNAGDQSYLPFFRGQCIHPALSEAVVKA
                     FGALADPDHARYYDG"
     gene            complement(1537..2487)
                     /locus_tag="PLJ11_04015"
     CDS             complement(1537..2487)
                     /locus_tag="PLJ11_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOO03868.1"
                     /translation="MRSKADVHVHTRYSGFGQYGVITFPESVCPPEAVVDEARRKGMR
                     VLCITDHDSVRGALRAREYAKRFDDLEVVIGSEISAAEGEVIGLFIEEEVPQGLPVRE
                     TIDRIRAQGGLVVAPHPFSCHVPALRDLVDELEIDGFEVLNGGHVDGYANDAAKNHAM
                     NGRWARLAGSDAHSTGQIGCCYTTFEGETAEDLYKAILAKATAAEGEVATLQMGIKWT
                     VQVVLNTDLLILRSLFHPLKSDDPEDPLVKKINIMRGENKLAALISSSIYLTPPIPIL
                     TGVTGVKVMRKLNRSNGFHQDGVSRRNNGHGLGRPGPRRP"
     gene            complement(2565..2684)
                     /locus_tag="PLJ11_04020"
     CDS             complement(2565..2684)
                     /locus_tag="PLJ11_04020"
                     /inference="COORDINATES: protein motif:HMM:NF018140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HOO03869.1"
                     /translation="MTKVGEVYECEICGNKVKVVEAGKGTLVCCGQPMTKVGG"
     gene            complement(2713..3885)
                     /locus_tag="PLJ11_04025"
     CDS             complement(2713..3885)
                     /locus_tag="PLJ11_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOO03870.1"
                     /translation="MEAVEIADGIYWVGALDWNCRSFHGFATPRGTSYNAYLLLDERN
                     VLIDSVKAPFMEEMMARVRSVIDPREIDLIVSNHAEGDHASGLPMLQELTGAEVLASK
                     RGVEALALNYGRLRMREVADMEELRIGRRTLRFIETPMVHWPESMFTYVVGDGVLFSM
                     DAFGQHYCTSQRFDDQMDPGQLLEEAATYYANIVLPFGAQVKRAYEKVKGLPLRLLAP
                     SHGVLWRAHIEEIVQAYLGWAEGRTEERVLIIYDTMWGSTERMAMAMAEGVRSQGVPV
                     VVMRLTDTDRSMVMREVLRSRVIAVGSCTMNNGMYPTVADITTYMMGLRPKGRKGAVF
                     GSYGWGGGASKAIHESLESAGLEIPYPDLEIRFAPMGEGVDRCVEYGAAIAKSIKS"
     gene            3963..4196
                     /locus_tag="PLJ11_04030"
     CDS             3963..4196
                     /locus_tag="PLJ11_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03871.1"
                     /translation="MNEGLVLLMEALQEVMGDGRYTTREMEDLLFTYFAYRCPDDLSR
                     SLNKLRLMGMIDGEVDRERACWVWWAKPKAHPE"
     gene            complement(4184..5953)
                     /locus_tag="PLJ11_04035"
     CDS             complement(4184..5953)
                     /locus_tag="PLJ11_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HOO03872.1"
                     /translation="MRIAVLLRDRCQFKKCNQECLRYCPKVRTGVEAVVIGEDGRPVV
                     SEELCAGCGICVHKCPFEALKIIQLPDEIRNDIVHQYGLNAFRLYGLPVPKEGVVTGI
                     LGPNGIGKSTSFRLLSGEEVPNLGNYEHPPSKEEVLEHFKGTEMHDHLKRVYDNRVRT
                     SIKPQYVDKLPNVFKGEVRALLKKVEGRMAMDEAVALLELEEAIDRQISELSGGELQR
                     VAIAATIMKDADIYFFDEPSSYLDIYQRIKVARTIEELARDRQVIVIEHDLAILDFLA
                     ENVYLVFGSEGAFGIYAQPRQVRQAINVYLGGYAKEENMRFRDAPIVFEARPPRSNWE
                     QFNLLEYGGIECRYPSFHLKVEPGAIKIGETVGVVGPNAIGKTTFVKVLAGVQQPTVG
                     KIDSSFKVSYKPQYISPDFDGTVREMFHATVKDFFESGFFQSEIARPLSLKNLLEKNV
                     LTLSGGELQRVAIASCLSREADIYLIDEPSAYLDSNQRMEAAKTVRRVMEKRGRSALV
                     VDHDIYFLDMVSDSIMVFSGTPGKEGLGQGPMDMRTGMNAFLKGVDVTFRRDNDTNRP
                     RINKPGSRLDREQKERGEYYYSG"
BASE COUNT         1147 a         1934 c         1850 g         1183 t
ORIGIN      
        1 agccgccgcg ttggcgatct gtcctacatt gcatccgccg gagcaggaaa agaacacgag
       61 ttcctttcag ctgcattggc actccacatt ctcagtcatg ttcccctaac ggcaagggac
      121 gtccgctgaa ggcgaccctc gggtccttga atatatatta agggcgcggc gccctaggtg
      181 gagcgggtct cagcatgaag gagtacgatc tcatcgtcat agggtccggg gcgggactga
      241 acgtggtggc caaggccagg cagaggaaga tgaaggtggc cttggccgag aacgggccga
      301 tgggcggcac atgcctcaac cggggctgca tccccagcaa gctattggca actccggcgg
      361 agctcgtgcg caccatcgcc gatgccaagc gcatcggcat ccacgccacg gtggagaagg
      421 ccgacttcca gttcgtgcgg gagcgcatgt ggaagctgat cgctccggac cgcgacggca
      481 ttctggagag catacggagg gacagcggca tcgacctgtt ccagacgacg gtccattttg
      541 ttgggaggaa cacgctgcag gcaggggacg agcagatcac ctccaaaagg atcgtcattg
      601 ccgctggtgc aaggaccaag gtccccaatg tcccggggct cgcggaggca gggttcctca
      661 ccacggagaa cttcttcgag ctttcggagc taccggagag catgctcatc ctgggcggag
      721 gatataaggc atacgagctc ggaatgttcc tggcatcctt cggctgcagg acgaccatca
      781 taggccgcaa ccccaggctc ctgaagggcg aggaaccgga gataagcgcc atcgtgctca
      841 agagggccag ggagtacatg gacgtgctcg tcgatcagga ggtgctcgcg gtgcgcaggg
      901 aaggcagccg gaaggtggcc atcgtaaggg acaggtccag cggagcaggg cgggaggtag
      961 gcgcggagca gatcctggtg gcgaccgggg tccagggcaa cgcggactgg ctccgccccg
     1021 aggccgccgg gatagccgtt gacgagggga actacgtcct cgtggacagg tatcttgaga
     1081 ccaatgtccc aggcatcttc gccatcgggg acatcatcgg ccgcaccatg ttccgccata
     1141 cggccaacta tcattccgag ctcgtctggc ataacatgtt cagcgagaaa cgcgccgagc
     1201 tggacgagca cgccgtgccc cacgccgtct tcggatatcc ggaggtgggg agcgtcgggc
     1261 tcaccgaaga ggagtgcaag cggcgggggc acaccatcct tgttggatac agtgagtaca
     1321 tggactgcgc gaaggggtac gccatgggcg aggaggacgg gctggtcaag gtcatcgtgg
     1381 acaaggggag ccaccgaatc ctcggcgcgc acatagtggg cgcgcacgcg gccctgctgg
     1441 tgcagcaggt ggtgtacctc atgaacgcgg gggaccagag ctatctgccg ttcttccgcg
     1501 gccagtgcat ccatccggcc ttgagcgagg cggtggtcaa ggccttcggg gccctggccg
     1561 acccagacca tgcccgttat tacgacggct gaccccgtcc tgatggaaac cgttgctccg
     1621 gttcagcttt cgcatgacct tcacccccgt gaccccggtc aaaatgggga tcggcggggt
     1681 taggtagatc gacgaggaga tcagggcagc caacttgttc tctccccgca tgatgttaat
     1741 cttcttgacg agggggtcct cggggtcgtc gctcttaagg ggatggaaga gcgagcggag
     1801 gatgagaagg tcggtgttga gcaccacctg caccgtccac ttgatgccca tctggagggt
     1861 ggcgacctcg ccctcggcgg cggtcgcctt ggccaggatg gccttgtaaa ggtcttcggc
     1921 cgtctcccct tcgaaggtgg tgtagcagca cccgatctgc ccggtggaat gggcatcgct
     1981 ccccgccagc ctggcccatc tgccgttcat cgcatggttc ttggccgcgt cattcgcata
     2041 cccgtccacg tgccctccgt tcagcacctc gaacccgtct atctccagct cgtccaccag
     2101 gtcccgaagg gcgggaacat ggcaactgaa gggatggggg gcgaccacta accctccctg
     2161 agcccttatc cggtcgatag tctcgcgcac cgggagaccc tgtgggacct cctcttcgat
     2221 gaaaagaccg atgacctccc cctccgcggc agagatctcc gaaccgatca ccacctcgag
     2281 gtcatcaaag cgttttgcgt attccctggc cctgagcgcc ccgcgcacgg aatcatgatc
     2341 ggttatgcag aggacccgca tacccttcct gcgggcctcg tcgaccaccg cctctggagg
     2401 gcagaccgac tcggggaacg tgatgacgcc atattgacca aagccggagt agcgggtatg
     2461 gacgtggacg tcggccttgc ttcgcacggg ggaacgaacc acacaacttg tttaattagt
     2521 ttcgcaccca gtgggaaaat gggtaggggc gggcatacgc cgcatcaccc gccgaccttg
     2581 gtcatgggct gtccgcagca taccaatgta cctttgccag cctccacgac cttgaccttg
     2641 ttcccgcaga tctcacattc gtagacctct cctaccttcg tcatttttgc acccattctg
     2701 ggaacacagt ccttaggact taatgctttt cgcgatggcc gcgccgtact cgacgcatcg
     2761 gtccaccccc tcccccatag gagcgaacct gatttccagg tccggatacg ggatctcgag
     2821 cccggccgat tcgaggctct catggatggc cttggaggcc cctcctcccc acccatagct
     2881 gccgaagacc gcgcccttgc ggcccttggg cctcaatccc atcatgtatg tcgttatgtc
     2941 ggccacagtg gggtacatcc cgttgttcat ggtgcaggag ccgaccgcta tcaccctgga
     3001 gcggaggacc tcccgcatga ccatggagcg gtcggtgtcg gtcagccgca tcacgaccac
     3061 cggaacgccc tgggaccgca ccccctcggc catggccatg gccatgcgct ccgtggagcc
     3121 ccacatggtg tcatagatta tcagaacgcg ctcctccgtc ctcccctccg cccagcccag
     3181 ataggcctgg acgatctcct cgatatgcgc cctccacagc acgccatggg agggggccag
     3241 cagcctcagg gggagcccct tcaccttctc gtacgctctc ttgacctggg ccccgaaggg
     3301 caggacgatg ttggcgtaat aggtagcggc ctcctccagc aattgtccgg gatccatctg
     3361 atcatcgaag cgctgcgagg tgcaatagtg ctgcccgaag gcatccatgc tgaacagcac
     3421 cccatccccc acgacatagg tgaacatgga ctccggccaa tggaccatgg gggtctcgat
     3481 gaaccgcagg gtgcgtctgc cgatccgtag ttcctccata tccgccacct ccctcatccg
     3541 cagccttccg tagttcagcg cgagggcctc cactcccctc ttggaggcca ggacctctgc
     3601 tccagtcagt tcctggagca ttggcagacc gctcgcatgg tcgccctccg catgattgga
     3661 gacgatgagg tcgatctccc gggggtcgat caccgaccga acacgggcca tcatctcctc
     3721 catgaagggc gccttcacag agtctatgag gacgttccgc tcatcaagaa ggaggtaggc
     3781 gttgtaggac gtcccccgag gggtagcgaa gccatgaaag ctacggcagt tccaatccaa
     3841 cgcccccacc caatagattc catccgcgat ctcgaccgct tccatgaccc cttcattcaa
     3901 ttctacggat atacattgtt ggcgagaaac ctttttgtgg acatgccccg cattaccgta
     3961 ggatgaacga ggggctggtc ctgctcatgg aggcgctcca ggaggtcatg ggcgacgggc
     4021 gctataccac cagggagatg gaggacctgc tcttcaccta cttcgcctat cggtgcccgg
     4081 acgacctctc ccgctccctc aacaagctcc gccttatggg gatgatcgac ggcgaggtgg
     4141 accgggaaag ggcctgctgg gtgtggtggg caaagcccaa ggctcacccc gagtagtagt
     4201 attcccctct ttccttctgc tcccggtcca aacgggagcc gggcttgttg atgcgcgggc
     4261 ggttggtgtc gttgtcccgg cggaacgtga catccacgcc cttcaggaac gcattcatgc
     4321 ccgtgcgcat gtccatcggc ccctgcccca gcccctcctt ccctggggtc ccgctgaaga
     4381 ccatgatgga gtcggagacc atgtccagga agtagatgtc atggtcgacc accagggcgc
     4441 tccgtccccg cttctccatc accctcctga cggtcttggc cgcctccatc ctctggttgg
     4501 agtccaggta ggcggacggc tcgtcaatga ggtagatgtc cgcctcccgg gagaggcagg
     4561 aggcgatggc cacccgctgc agctccccgc cggagagggt cagcacgttc ttctccagca
     4621 ggttcttcaa cgacaatggt ctggcgatct cgctctggaa gaagcccgac tcgaagaagt
     4681 ccttcaccgt ggcatggaac atttcgcgga cggtgccatc aaagtcgggg cttatgtact
     4741 gcggcttgta gctgaccttg aagctgctgt cgatcttccc gacggtgggc tgctggacgc
     4801 ccgccagtac cttcacgaag gtggtctttc ctatggcgtt cggtccgacg accccgacgg
     4861 tctccccgat ctttatggcc cccggctcca ccttgaggtg gaaggagggg tagcgacatt
     4921 ctatgccccc atactccagg aggttgaact gttcccagtt ggaacgggga ggccgggcct
     4981 cgaacacgat cggagcgtcg cggaatcgca tgttctcctc cttggcgtag ccgccgaggt
     5041 atacgttgat ggcctgccgc acctgcctcg gctgcgcgta gatgccaaac gccccctccg
     5101 acccgaagac aagatagaca ttctcggcca ggaagtccag gatggcgagg tcgtgctcga
     5161 tgacgatgac ctgcctgtcc ctggccagtt cctcgatggt ccgggcgacc ttgatcctct
     5221 ggtatatgtc caggtaggag gagggctcgt caaagaagta gatgtcggcg tccttcatga
     5281 tggtggcggc gatggccacc ctctgcagct cccccccgga cagttcggag atctggcggt
     5341 ctatggcctc ctccagctcc aggagtgcga ccgcctcgtc catggccatc cgcccctcca
     5401 ctttcttgag gagggcgcgg acctcgccct tgaacacgtt gggaagcttg tcaacgtact
     5461 gcggcttgat ggacgtcctc accctgttgt cgtagacccg cttgaggtgg tcgtgcatct
     5521 ccgtgccctt gaagtgctcc aggacctcct ccttgctcgg ggggtgctcg tagttgccga
     5581 ggttcgggac ctcctctccg gagagcagcc ggaacgaggt gctcttgccg ataccgttcg
     5641 gaccaagtat gccggtcacc acgccctcct tgggcactgg cagaccgtag agtcggaagg
     5701 cgttcaatcc atactggtgc acgatgtcgt tgcggatctc gtccgggagc tggattatct
     5761 tgagggcttc gaacggacat ttatgcacgc agatgccgca gcccgcgcac agctcctcgg
     5821 agaccacggg ccggccgtcc tccccgatga ccaccgcctc cacccccgtt cgcaccttgg
     5881 gacagtacct caggcactcc tggttgcact tcttgaactg acacctgtcc ctcagcagga
     5941 ccgctatgcg catgccccca cctattgaaa ggaggcataa aaaagtaatg ggcgcgtggg
     6001 gatgagggct cagatggcca aggagaccgt gaggcgcctt acgccctcaa tataaggccc
     6061 attggtcccg aggagccggg agacatcggg ctcgttctcc cggaccgtag ggtc
//
LOCUS       DAOSYN010000069         6082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10947_length_6082_cov_5.137548, whole genome shotgun sequence.
ACCESSION   DAOSYN010000069 DAOSYN010000000
VERSION     DAOSYN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10947_length_6082_cov_5.137548"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..247)
                     /locus_tag="PLJ11_04040"
     CDS             complement(122..247)
                     /locus_tag="PLJ11_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03873.1"
                     /translation="MSLERMAEQQAEGGGAMYRFKNGIFKDNKLTIKEKGFIAIA"
     gene            326..868
                     /locus_tag="PLJ11_04045"
     CDS             326..868
                     /locus_tag="PLJ11_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03874.1"
                     /translation="MEPVNMAVEIIKGMPGTCMVFPLSEEVIKDIQRVEGGIRSQIGL
                     DVVNEGLNQCLKRQHVICVIKDSRFRPPPEPTVLMMGDTDMVIGTEVLAGQHSRYKDM
                     ENVCWLGNDFVVFTDKVPRTKEYFLMPPVPFPELEQVPGARNIVSCSPSPLGDLIIKN
                     HYNIKDDPKLASILVGFDTS"
     gene            complement(1186..1455)
                     /locus_tag="PLJ11_04050"
     CDS             complement(1186..1455)
                     /locus_tag="PLJ11_04050"
                     /inference="COORDINATES: protein motif:HMM:NF021397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 family protein"
                     /protein_id="HOO03875.1"
                     /translation="MQVGEFAKYRVTGTVGKVLEVRERDGVEWALLDTYDLWYVTSLL
                     DPASEDEYKRTSYKERTRKVVLGEMQGLTEEVVADISTVTPSGSG"
     gene            complement(1464..3128)
                     /gene="mcrA"
                     /locus_tag="PLJ11_04055"
     CDS             complement(1464..3128)
                     /gene="mcrA"
                     /locus_tag="PLJ11_04055"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     alpha"
                     /protein_id="HOO03876.1"
                     /translation="MAKEKDKLFIAAMKKKFKEDPTEVRSKHYSYGGWRQSKRKREWV
                     EQANRIAKERGIPMMNQDIGVALGQRVLMPYQLSHTDIYGEADDLHFVNNAAMQQAWD
                     DIRRTVIVGLDVAHNVIEKRLSKEVTPETINRYLEAVNHTMPGGAVVQEHMAELCPEI
                     VKDSYVKVFSGDDELIDEIDKCFVIDINKEFPADQAKQLKAAVGKKLWQVVRVPTIVG
                     RVCDGATVSRHSAMQISMAFITAYRLAAGEAAIADFAFAAKHAAMVEMATMMPARRAR
                     GPNEPGGLPFGVLADCVQSTRTKPDDPARASLEAVALGAVILDQIYLGSYMSGGVGFT
                     QYATAAYTDNILEDYTYWAVDHIKEKYGGFCKSKPSTELIEKLGSEINSYALEMYERY
                     PAAMEAHFGGSQRATVAAAATGIGVAFATGNANAGINGWYLSMYQHRERLGRLGFYGY
                     DLQDECGAANSFSYRSDEGLPHELRGPNYPNYAMNVGHLSGYTGIALAPHAARGDAFV
                     ASPLVKIAFADKNLPFDFANITKEFGRGCLREFMPMGERTAIIPAR"
     gene            complement(3131..3913)
                     /gene="mcrG"
                     /locus_tag="PLJ11_04060"
     CDS             complement(3131..3913)
                     /gene="mcrG"
                     /locus_tag="PLJ11_04060"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HOO03877.1"
                     /translation="MAKTKYKAQFYPGTTLPAKNRRRFMDPSVKLEKLRDIPMDDVVK
                     ILGHRVPGSDYATIHPPLEEGNEPDCPIRAIVEPTEGAKKGDRIRYVQFTDSVYWAPI
                     VPYVRAQMYMWRYRGIDTGTLSGRQIIEMRERDVEKVAKELIDSETFDTARTGIRGAT
                     VHGHAVRLDENGLMFDAWQRFRWNAKKGEVEYVKDQVGVPLDKPVPVGKPMPEKWLKE
                     HTAMFRADGVDMREDEEVMRQNLRIHTLRTKCGFKPFDVKED"
     gene            complement(3915..4337)
                     /gene="mcrD"
                     /locus_tag="PLJ11_04065"
     CDS             complement(3915..4337)
                     /gene="mcrD"
                     /locus_tag="PLJ11_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase operon protein D"
                     /protein_id="HOO03878.1"
                     /translation="MSAPASEGVPLPEIQIFPERLLMASTTEKLLNRLYDIKNVRQIN
                     IQGEGLPAVMKSGPGTGGPVDHPERKKIKVKGKEVELTVQVGRIFVEISDIDHVPQAL
                     KDIEAVCKEVLPFGFTLEVGRYNKFRPTTTDYMKGLVK"
     gene            complement(4340..5668)
                     /gene="mcrB"
                     /locus_tag="PLJ11_04070"
     CDS             complement(4340..5668)
                     /gene="mcrB"
                     /locus_tag="PLJ11_04070"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HOO03879.1"
                     /translation="MAKYKDKVDLYDDRGKLIEKDVPLDAISPLNNGAIMRTISLTKR
                     TIAVNLEGIEKSLKTGAVGGLNVPGRGMELAIVANADKLAKKLKDTLRVSKDDDTEIK
                     VMADGKRMVAMCPSARIDTGAEYTNGFTAVAAALTEAIIDMFDVGMFEASMVKAAVWG
                     RYPQTIGMAGSNLKSILDLPQFNEGTGYALRNIMANHIVMLTGRNAMNAAALSSIYET
                     VAAYEMGDAIGNFERQHLLSLAYQGLNGNNMVYTWVKENGKTGTVGDVVASVVGTALE
                     DKVIKKGKKYPSGFVEYTTNDFPLWNAYISAGMLAAICVNIGAARAAQGVPSTILYYN
                     DLIEHETSLPGVDYGRAMGTSVGMSFFSHSIYGGGGPGLFHGNHVVTRHAKGTVVPVI
                     AAGCALDAGTQYFSAENTSLIMGEVFGKLDEFKSPMKMVGKEAKRLKELV"
BASE COUNT         1121 a         1866 c         1657 g         1438 t
ORIGIN      
        1 cgctcggccc cgccaggtac atggccacga acaacgcctc gcggtcctcg tccttggtcg
       61 cccccgcccc cggggccttc ggaatgtggc cagaggcatt cgtcgcactt cagcaccgcg
      121 gtcacgcgat tgcgatgaac cccttctcct tgatggtcag cttattgtcc ttgaagatcc
      181 cgttcttgaa gcggtacatc gccccaccgc cctcggcctg ttgctcggcc atccgttcca
      241 gggacatgaa tgggggatct gcctgaggaa tattgagcga ccggggaaac aatctttttc
      301 tagcgtgaag gccttagggg ttggcatgga acctgtcaat atggccgtgg agatcatcaa
      361 gggcatgccc ggcacctgca tggtcttccc gctttccgag gaggtcatca aggacattca
      421 aagggtcgag ggcggcatca ggtcccagat aggactggac gttgtgaacg aggggctgaa
      481 ccagtgcctt aaaaggcagc atgtcatctg tgtgatcaag gattcccgtt tcaggccccc
      541 tccggaacct accgtgctta tgatgggcga tacggacatg gtcatcggga ccgaggtcct
      601 tgctggtcag cactcccggt acaaggacat ggagaacgta tgctggctgg ggaatgactt
      661 cgtcgtcttc acggacaagg tccctaggac caaggagtac ttcctaatgc cccctgttcc
      721 cttcccggag ctggaacagg tcccgggggc gaggaacatc gtctcctgca gcccttcccc
      781 gttaggggac ctgatcatca agaaccatta caacatcaag gacgatccga agctggccag
      841 catccttgtc ggctttgaca cgtcctaaac ctttattctg attccatagg agcgagaggt
      901 tctcgtcgcc acagccctga tgagccctgg cccaattacg ctccggcccg tgcctccgtc
      961 agccgcaccg gtcaggaata tgaccagggt acagacgatc tacggcgtgt ccttcgacga
     1021 tgcccccgat tgcttagctc gccctgagcc atctccatct aaggcgatct gcatgtacca
     1081 gcacggcaag agaggggttc gtgatcaagc agtccgcaag gaataatggc tgtccagaat
     1141 taagaaaaaa aaagatgacg ggaaagggtt ttgtgcttgg ttggctcaac cgctcccgct
     1201 cggggtcacg gtggagatgt ccgccacgac ctcttcggtc aggccttgca tctcccccag
     1261 gaccaccttt cgggtccttt ccttgtagga ggtccgcttg tactcgtcct cggatgcggg
     1321 gtccaggaga ctggttacgt accagaggtc gtaggtgtcg agcagagccc attccacccc
     1381 gtcgcgctcc cgtacctcca gcacctttcc caccgtcccg gtgacgcggt attttgcgaa
     1441 ctctcctact tgcatcggac acctcaccta gcagggatga tcgcggtcct ctcgcccatt
     1501 ggcatgaact ccctcaggca tccgcgaccg aactccttgg tgatgttcgc gaagtcgaag
     1561 ggcaggttct tgtctgcgaa ggcgatcttg accagcgggc tggccacgaa ggcatcgccg
     1621 cgggcggcgt ggggtgccaa tgcgataccg gtgtatccgc tcaggtggcc cacgttcatc
     1681 gcgtagttcg ggtagttcgg accgcgcagc tcatgcggta ggccctcgtc gctcctgtag
     1741 gagaacgagt tggccgcccc gcattcgtcc tgcaggtcgt atccgtagaa cccgagcctt
     1801 cctagcctct cccggtgctg atacatcgac aggtaccagc cattgatccc tgcgttggcg
     1861 ttaccggtgg cgaaggccac acctatgccg gtggcggccg ctgcgacggt ggccctctgg
     1921 gaccctccga agtgcgcctc cattgcggcc gggtagcgct cgtacatctc caaggcgtag
     1981 gagttgatct cggagccgag cttctcgatc agctcggtgg agggtttgga cttgcagaac
     2041 ccaccgtact tctccttgat gtggtccacg gcccagtagg tgtaatcctc caggatgttg
     2101 tcggtatagg cggcggtggc atactgagtg aacccgacgc ccccggacat gtaggacccg
     2161 aggtatatct ggtccagaat caccgcgccc aaagcgacag cctccaggga ggctctagca
     2221 gggtcatccg gcttggtcct ggtggactgc acgcagtccg ccagcacacc gaacgggagc
     2281 ccaccgggct cgttggggcc cctggccctc ctggcgggca tcatggtggc catctccacc
     2341 atcgcggcgt gcttggcggc gaaggcgaag tccgcgatcg cggcctcgcc ggctgccagc
     2401 ctgtaggcgg tgatgaaggc catggagatc tgcatcgccg agtgcctgga gaccgtggct
     2461 ccgtcgcaca cgcggccgac aatggtcggg acgcgcacta cctgccacag cttcttaccg
     2521 accgcggcct tcagctgctt ggcctggtcc gcggggaact ccttgttgat gtcgatgacg
     2581 aagcacttgt cgatctcgtc gatgagctcg tcgtccccgg agaacacctt gacgtaggag
     2641 tccttcacga tctcggggca tagctcggcc atgtgctcct gcaccactgc gccgcctggc
     2701 atggtgtggt tgacagcctc caggtaacgg ttgatggtct cgggggtgac ctccttcgac
     2761 agcctcttct cgatgacatt gtgggcgacg tccagaccga cgatgacggt ccttcgtatg
     2821 tcatcccacg cctgctgcat cgcagcgttg ttcacaaagt gcaggtcatc ggcctcgcca
     2881 tagatgtcgg tgtgggacag ctggtagggc atcaagaccc tctggccgag cgcgacaccg
     2941 atatcctggt tcatcattgg gatcccgcgc tccttggcga tcctgttggc ctgctcgacc
     3001 cactcccgct tcctcttgga ctgcctccag cccccgtagg agtagtgctt ggagcggacc
     3061 tccgtggggt cctccttgaa cttcttcttc atggcggcga tgaacagctt atccttttcc
     3121 ttcgccatat ttagtcctcc ttaacatcga agggcttgaa gccgcacttc gttctcaagg
     3181 tgtggatcct gaggttctgc ctcatgacct cctcgtcctc cctcatgtcg acgccatcgg
     3241 cgcggaacat ggcggtgtgc tccttcagcc acttctcggg catgggcttt ccgacaggga
     3301 ccggcttgtc cagcgggaca cccacctggt ccttgacgta ctccacctcg cccttcttgg
     3361 cgttccaccg gaacctctgc caggcatcga acatcagccc gttctcgtcc agcctcacgg
     3421 cgtggccatg cacggtggcg cctctgatac cggtcctcgc ggtatcgaag gtctcgctgt
     3481 cgatgagttc cttggccacc ttctcgacgt ccctctccct catctcgatg atctgccttc
     3541 cagatagggt accggtgtca atccctctgt acctccacat gtacatctgc gccctaacat
     3601 acggaactat gggggcccag tatacggagt cagtgaactg aacgtacctg atcctgtctc
     3661 ccttcttagc accttctgtg ggctcgacga ttgcccttat ggggcagtcg ggctcgttgc
     3721 cctcctccag cggcgggtgg atggtggcgt agtcggaacc ggggaccctg tgccccagga
     3781 tcttgaccac gtcgtccatc ggaatgtcac ggagcttctc cagcttgacg ctggggtcca
     3841 tgaagcgcct gcggttcttg gcgggcaggg tggtgccagg atagaattgt gccttatact
     3901 ttgtcttagc catgttactt caccaatcct ttcatgtagt ctgtagtagt gggcctgaac
     3961 ttgttgtaac gacctacctc caatgtgaac ccgaagggta gcacttcctt gcataccgcc
     4021 tcgatgtcct tcagggcctg cggcacatgg tcgatgtcgc ttatctcaac gaatatgcgc
     4081 ccgacctgga ccgtaagctc gacctccttt cccttgacct tgatcttctt gcgctcggga
     4141 tggtccaccg gaccgccagt gccaggaccg gacttcataa ccgccggtag cccctcgccc
     4201 tggatgttta tctgtcgaac gttctttatg tcatacaatc ggttcagcag cttttcggtg
     4261 gtgcttgcca tcaaaagtct ttccgggaag atctggatct ccggaagcgg gactccctcg
     4321 gaggcaggtg cgctcatgtt cagaccagct ccttcagcct cttggcctcc ttaccgacca
     4381 tcttcatagg gctcttgaac tcgtcgagct tgccgaacac ctcacccatg atgagggatg
     4441 tgttctcagc gctgaagtac tgcgtaccag cgtccagagc gcatccggca gcgataacgg
     4501 gcacaaccgt tcccttggca tgcctggtaa cgacgtggtt cccatggaac agccccgggc
     4561 cgcccccgcc atagatcgag tggctgaaga aggacatgcc gaccgaggtc cccatggccc
     4621 tcccatagtc cacaccgggc aggctcgtct cgtgctcgat caggtcgttg tagtacagga
     4681 tcgtggatgg aacgccctgc gcagcccttg cggcaccgat gttcacgcat atcgcggcga
     4741 gcataccggc ggagatgtag gcattccaca gcggaaagtc gttggtggtg tactcaacga
     4801 atccggacgg atacttctta cccttcttta tgaccttgtc ctccaaggcg gtcccgacca
     4861 cggaggcgac gacgtctccg acggtgccgg tcttgccatt ctccttgacc caggtataga
     4921 ccatgttgtt gccgttcaat ccttggtagg ccagggacag caggtgttgt ctctcgaagt
     4981 tgccgatggc atcgcccatc tcataggccg cgacggtctc gtagatcgaa gacagagcgg
     5041 ccgcgttcat cgcgttcctt cccgtcagca tgactatgtg gttggccatg atgttcctga
     5101 gcgcgtagcc agtaccctcg ttgaactgcg gaaggtccag gatggatttc aggttcgagc
     5161 ccgccatacc tatggtctga ggatatctgc cccaaacggc ggccttcacc atcgaggcct
     5221 cgaacatgcc cacatcgaac atgtcgatga tcgcctccgt aagggcggca gcgacggcgg
     5281 tgaatccgtt ggtgtactcg gccccagtgt cgatcctggc gctcgggcac atcgcaacca
     5341 tcctcttccc gtccgccatg accttgatct cggtgtcatc gtccttggag accctcagtg
     5401 tgtccttcaa cttcttggcc agcttgtccg cgttcgccac tatcgcgagt tccatgcccc
     5461 ttccggggac gttcagtcca ccaaccgctc cggtcttgag gctcttctca ataccctcga
     5521 ggttgacagc gatcgtcctc ttggtcaggg agatggttcg cataatggcg ccgttgttca
     5581 acggactgat ggcgtccaga ggcacatcct tttcaatcag cttgcctctg tcgtcataca
     5641 agtccacttt gtccttgtat tttgccattt tatacctcct tttcaatagc tcggtcccct
     5701 caagtagatt cagcatcgat cttgagaaaa ccggtacccc ttgcggactc gacaatgtta
     5761 agttagaata taagcttgat gatggatgga taatccatcc atgttaaaca atagcttaat
     5821 ttgagtcgtg ttccagacaa aaatgcatct gttttaacat cctaaatttt ttttaatatt
     5881 atttatagtt aatataaata atgtttcatt attaatttat ttttcaattg ttcttataat
     5941 attgatcccc gtcattcgct catctttttt agatgtctca ggacggctat tgcgtcctcc
     6001 ttcttaacat ctcggtagtt ctccgcgaaa aaccggtcga aaccctccgc cctaatgtcc
     6061 ccaaggacct cccttgcgat gc
//
LOCUS       DAOSYN010000070         6077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10958_length_6077_cov_4.181999, whole genome shotgun sequence.
ACCESSION   DAOSYN010000070 DAOSYN010000000
VERSION     DAOSYN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10958_length_6077_cov_4.181999"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLJ11_04075"
     CDS             <1..347
                     /locus_tag="PLJ11_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03880.1"
                     /translation="LASDLRWDSLRYLLRGAGAPYVSLLGLDMLEAVYGGNVVPKLLQ
                     HVDQMRRGGHIVVVEATQGSAGLEALSHQARLHFRLEDINGSVMVRGIKPSTLYHCLD
                     INEGVPLLRPML"
     gene            408..749
                     /locus_tag="PLJ11_04080"
     CDS             408..749
                     /locus_tag="PLJ11_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03881.1"
                     /translation="MVKGALNLSRLLTDEYAGRILSLTFSQARSVQEICAISGIPIAV
                     AYRRVAALEAAGLLRCLRTEIAPSGKKCKYFICQVQVARLTFREGSFEVEVEWKDQGR
                     EELAPMPSFRT"
     gene            788..1156
                     /locus_tag="PLJ11_04085"
     CDS             788..1156
                     /locus_tag="PLJ11_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOO03882.1"
                     /translation="MARNDLLGKSRLLTDEYAVKILMATVRSPRSAQQISEQYGIPIA
                     ACYRRIRDLEMVGLLHCTERRLSSQGKRVCFYQSRLRTASIQYENGRLRVRFTMQDGE
                     TDSEWQDVDLEGGGGEDDRR"
     gene            1276..2148
                     /locus_tag="PLJ11_04090"
     CDS             1276..2148
                     /locus_tag="PLJ11_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HOO03883.1"
                     /translation="MTELLEDLDQKRQFNNNEAERHRRLRDELNDKTKEWVQKRDELN
                     AKVRELVEAAAKHRENRDKLNEQVRAAKEARDKLNEQVSELNKKVMALKKDNVPQEGP
                     SVAKLKKDLKQLEFIHMTSGDLKRDKEKALVEQMKALQIQIREREKALEANDEVRQAI
                     AQLREAKEKAEEQHRLVSELAEAAQNEHDAMIKIYEEADKLRKEADEAQERFIETKAK
                     ADEEHRRHIDHIRQVHDYDKIITGLRQKARKARKKKDESVAMKEAEDIFDKFKKGEKL
                     STEDLMVLQKSGYL"
     gene            2397..3740
                     /locus_tag="PLJ11_04095"
     CDS             2397..3740
                     /locus_tag="PLJ11_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein Srp54"
                     /protein_id="HOO03884.1"
                     /translation="MVLEGLGQSLRDALRKVAGSSNVDEKLIKEVSKDIQRALLQADV
                     NVKLVLELTKEMERRALTERPPAGKSAREHVIRIIYEELVKILGEQRTLPLGKQVIMM
                     VGLYGQGKTTTAGKLARYFHKKGLKVGLIAADVHRPAAYDQLKQIGDKVGVRVFGSPG
                     ERSAVKIVEAGMRELSGLDVIIIDTSGRHALEADLIEELKAVAKVANPDERILVMDAS
                     VGQQAGPQAQAFHDAVGITSVILTKMDGTAKGGGALSAVSRTKAPIVFIGVGEHLEDL
                     DPFIPARFISRMLGMGDIQSLIETAKETMTEEQALETTRKIMSGRFSLKEMYEQMEML
                     TNMGPLKKLMSMLPGLGGGLQDKVDVEETQERLRRYRIIMDSMTEEEMENPKLIKSSR
                     VMRIARGSGMDPKHVRELLQQYNTSKKAVRGLMGNRKLRKQLMKQMQGGQLEGLQ"
     gene            3737..4324
                     /gene="trmY"
                     /locus_tag="PLJ11_04100"
     CDS             3737..4324
                     /gene="trmY"
                     /locus_tag="PLJ11_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HOO03885.1"
                     /translation="MRRFIVIGHKAATAADFKLDDLAGGAGRLDVLLRCINSAFFLSH
                     DIRRDVEVVLILQGPPGPVRTIRLVGSELRYLNPDERSTGALIRNALMRDAKGEERST
                     PGIYVSARSFQDVLALLDKGTELVYLREDGQDIRGVELPKDVTFVLSDHMDLSPDEED
                     LLLQYGPRVLTLGPMSYHADHCITIVNNELDRRSL"
     gene            4362..4556
                     /locus_tag="PLJ11_04105"
     CDS             4362..4556
                     /locus_tag="PLJ11_04105"
                     /inference="COORDINATES: protein motif:HMM:NF021415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HOO03886.1"
                     /translation="MNQKIAQHRHCKACGKAFVGDGRYCSEECEKGNEQTMRRKRRQL
                     LTLYIASVIILIGALVMSVL"
     gene            4553..5512
                     /locus_tag="PLJ11_04110"
     CDS             4553..5512
                     /locus_tag="PLJ11_04110"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF014041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOO03887.1"
                     /translation="MTKVGVGGTFNVLHRGHRRLLDVAMENGDLVAVGLMSDAYCERN
                     KAVALPYERREAAVRAYLEGRGAVFTIAPIDEREGTAPSDPGLDVLVVSQETEAAGKG
                     INALRLGNGLRPVRIVTVPYVLADDYRPISSSRVLSGEIDQEGRLLRPLRVNVGSLNP
                     VKLAAARNVVQRFHPLLEVSAVAVPRRVGEQPWGQEAEEGAIARARECLGSADLGIGI
                     EAGVWERADGLYDVQYCAIIDAMGRVSIGHGMGFRYPPALAALVRQGLSVGDACAQLF
                     KEGDQGAGMGAIGILTNGVIDRQALTEQAVLAAMVPRIRKELY"
     gene            complement(5509..5745)
                     /locus_tag="PLJ11_04115"
     CDS             complement(5509..5745)
                     /locus_tag="PLJ11_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03888.1"
                     /translation="MSGCPQLGPDGKCQGLYLGFACIRDKCLMQDKEAACPHCIGGDY
                     CTKYNRFGCIGPENCATENDYLAHIRQARDKALG"
BASE COUNT         1312 a         1737 c         2027 g         1001 t
ORIGIN      
        1 acctggcgag cgatctgcgc tgggattcgc tgcggtacct gctgaggggg gcgggcgctc
       61 cttacgtctc cctactcggc cttgacatgc tggaggcggt ctacggcggc aacgtcgtgc
      121 ccaagctcct ccagcatgtg gaccagatga ggcggggcgg gcacatcgtg gtggtggagg
      181 cgacccaggg ctccgccggg cttgaggcgc tgagccacca ggccaggctg cacttccgat
      241 tggaggacat taacggttcc gtcatggtca gagggatcaa gccgtccacg ctctatcact
      301 gcttggacat aaacgagggg gttccgctcc tcaggcccat gctctgacat tatcagccat
      361 gatatccgcg gaatcggggt tatatccgaa cacggtcttg caggagcatg gtcaagggcg
      421 ccctcaacct gtcccgcctc ctgacggacg agtatgccgg caggatcctg agcctgacct
      481 tctcccaggc ccgttccgtt caggagatct gcgccatctc cggaatcccc atcgccgtgg
      541 cctaccgccg ggtggcagcc ctggaggcgg ccggcctcct ccgctgcctg cgcacggaga
      601 tcgccccgag cggaaagaaa tgcaagtact tcatctgcca ggtgcaggtg gcccgcctta
      661 ccttccgcga agggtcgttc gaggtggagg tggaatggaa ggaccagggg agggaggagc
      721 tggcgcccat gccttcattt agaacgtagc atgtttttta tgggcatagg gcaataccat
      781 aggccagatg gcgaggaacg accttctggg gaagtcaagg ctgctgaccg atgagtatgc
      841 ggtcaagata ctcatggcta cggtccgttc cccgcgcagc gcccagcaga tctccgagca
      901 atacgggatc cccatcgccg cctgctaccg caggatacgg gacctggaga tggtcgggct
      961 cctccattgc accgaacgcc ggctctccag ccaggggaaa cgggtctgct tctaccagtc
     1021 caggctccgg acagcctcca tccagtacga gaatggccgc ctgagggtcc gcttcacgat
     1081 gcaggatggg gagacggaca gcgaatggca ggacgtggac ctggaagggg gagggggcga
     1141 ggacgaccgg aggtgagcgc tccccatgta cactggacgg aacgcaggcc atatagaaaa
     1201 agttataaac aaaattcctt atgcctactc ccaggcttga gtcttatttg atttcaggat
     1261 tgagcgtgaa cgtttatgac agaactcttg gaggacctcg accagaagcg acaattcaac
     1321 aacaacgagg cggagaggca ccgccgacta agggacgaac tgaacgacaa gaccaaggaa
     1381 tgggttcaga aaagggatga gctcaacgcc aaggtccgcg agctggtgga ggcggccgca
     1441 aagcaccgcg agaaccgcga caagctcaac gagcaggtgc gggctgcgaa ggaggcccgc
     1501 gacaagctca acgagcaggt gagcgaactc aacaagaagg taatggccct gaagaaggac
     1561 aacgttccgc aggaggggcc gtcggttgcc aagctgaaga aggacctcaa gcagctcgag
     1621 ttcatccaca tgacctcggg cgacctgaag cgggacaagg agaaggccct ggtcgagcag
     1681 atgaaggccc tccagatcca gatccgggag agggagaagg cccttgaggc caacgacgag
     1741 gtccgccaag ctatagcgca gctgcgcgag gcaaaggaga aggccgagga gcagcacagg
     1801 ctggtcagcg agctggccga ggccgcccag aacgagcatg acgcgatgat aaagatctac
     1861 gaggaggcgg acaagctcag gaaggaggcg gacgaggccc aggaaaggtt catcgagacc
     1921 aaggccaagg ccgatgagga gcaccgccgg cacatcgacc atatccggca ggtgcacgat
     1981 tacgacaaga tcatcaccgg cctgaggcag aaggcccgga aggctcgcaa gaagaaggac
     2041 gagagcgtgg ccatgaagga ggctgaggac atcttcgaca agttcaagaa gggagagaag
     2101 ctcagcaccg aggaccttat ggtccttcaa aagtccggct acctctgaaa cccattaaca
     2161 ttcttcaccc tttgcccatc tggcatttgg gcatttcagt gcttttcccc atcttcaaca
     2221 aaaatggtaa agtatatata tcgtggaaaa gatagtagtg ttcgggaagg tctgggctgg
     2281 ttttcagagt gcatcccatc ccttctgaca ggacaggtcc ttcccaaacc cctttttctt
     2341 ccgttgtcca gggcaaaggt tatctaagcc ctcaggattg tcaatgaggg ctgaagatgg
     2401 tattggaagg gttggggcaa tcgcttaggg acgccctaag aaaagtggcg ggctccagca
     2461 acgtggatga gaagctgata aaggaggtgt ccaaggacat ccagagggcg ctcctccagg
     2521 cggacgtcaa cgtgaagctg gtcctggagc ttaccaagga aatggagcgc cgggcgctca
     2581 cggagaggcc ccccgcgggc aagtccgcgc gggagcacgt catcaggata atctacgagg
     2641 aactggtcaa gatcctcggc gagcagagga cgctccccct gggcaagcag gtgatcatga
     2701 tggtcggcct gtatggccag gggaagacca ctaccgccgg gaagctcgcc cgatacttcc
     2761 acaagaaagg gctgaaggtc ggcctgatag ccgcggacgt gcatcggccg gccgcctacg
     2821 accagctcaa gcagatcggt gacaaggttg gggtgcgcgt tttcgggagc cccggggagc
     2881 ggtcggcggt caagatcgtc gaggccggca tgagggagct cagcggcctt gacgtgatca
     2941 tcatcgacac ctctggcagg catgccctgg aggcggacct catagaggag ctcaaggcgg
     3001 tggccaaggt ggccaacccc gacgagcgca tcctggttat ggacgcgagc gttgggcagc
     3061 aggccgggcc gcaggcccag gcgttccacg acgcggtggg catcacctcc gtcatcctga
     3121 cgaagatgga cgggacggcc aagggagggg gggcgctgag cgcggtctcc cgcaccaagg
     3181 cccccatcgt cttcatcggc gtgggggagc acctggagga cctcgacccg ttcatcccgg
     3241 cacgcttcat ctcgcgcatg ctggggatgg gggacattca atcgctcatc gagacggcca
     3301 aggagaccat gaccgaggaa caggccctgg aaacgacccg gaagatcatg tccggcaggt
     3361 tctccctcaa ggagatgtac gagcagatgg agatgctcac gaacatgggc cccctcaaga
     3421 agctgatgag catgctcccc ggcctgggcg gggggctgca ggacaaggtc gacgtggagg
     3481 agacccagga gcgtctgcgc cggtaccgca tcatcatgga ctcgatgacc gaggaggaga
     3541 tggagaaccc caagctcatc aagtcctccc gggtcatgcg catcgcccgg ggctcgggca
     3601 tggaccccaa gcacgtccgg gaactactgc agcagtataa cacatccaag aaggccgtga
     3661 gggggctgat gggcaaccgc aagctgcgca agcagctcat gaagcagatg cagggcgggc
     3721 agctggaagg gttgcaatga ggcgcttcat cgtcatcggg cacaaggcgg ccaccgccgc
     3781 cgacttcaag ctggacgacc ttgccggcgg ggcggggcga ttggacgtgc tgctcaggtg
     3841 catcaactcc gccttcttcc tcagccacga catccggagg gacgtggagg tcgtgctcat
     3901 cctccagggc cccccggggc cggtgcgcac catccgcctg gtcgggagcg agctccgtta
     3961 cctcaacccc gacgagagga gcaccggggc cctgatccgg aacgccctga tgagggacgc
     4021 caagggggag gagaggagca cccctggcat ctacgtgagc gcgcggtcct tccaggacgt
     4081 gcttgcgctc ctggacaaag gcaccgagct cgtctatctc agggaggacg gtcaggacat
     4141 aaggggggtg gagcttccga aggacgtcac cttcgtgctc agcgaccata tggacctgag
     4201 cccggatgag gaagacctcc tcctccagta cgggccgagg gtgctcacgc tcggtcccat
     4261 gtcgtaccat gccgaccatt gcatcactat agtcaacaac gaattggacc gccggtccct
     4321 ctgagccaag ctttatattc ctcggaaaat gtgggagacg catgaaccag aagatcgccc
     4381 agcacagaca ttgcaaggcc tgcggcaagg ccttcgtggg cgatgggagg tactgctccg
     4441 aggaatgcga gaaggggaac gagcagacca tgcgcaggaa aaggagacag ttgctgaccc
     4501 tgtatattgc gagcgtcatc atattgatcg gcgcgctggt gatgtcggtg ctatgacgaa
     4561 ggtgggcgtc gggggaacgt tcaacgtgct ccatcgcggg catcgcaggc ttcttgacgt
     4621 ggccatggag aacggggacc tggtggcggt gggcctaatg agcgacgcct actgcgagcg
     4681 caataaggcg gtcgcgctcc cgtacgaacg gagggaggcg gcggtgcgcg catacctgga
     4741 aggacggggg gccgtcttca ccatcgcccc catcgatgaa cgggagggca ccgccccgtc
     4801 ggaccctggg ctcgacgttc tggtggtctc ccaggagacc gaggccgcag ggaaggggat
     4861 caacgccctg cggctgggga acgggctgcg tcccgtgcgc atcgtgacgg tcccctacgt
     4921 cctagcggac gactaccgcc ccataagctc cagccgcgtt ctgtcgggcg agatcgacca
     4981 ggagggccgg ctgctccgcc ccctcagggt gaacgtcggt tcattgaacc cggtcaagct
     5041 ggccgcggcc cggaacgtgg tccagcggtt ccatccgctc ttggaggtgt ccgcggtggc
     5101 cgtgccccgc agggtaggag agcagccctg gggccaggag gcggaggagg gggcgatcgc
     5161 ccgggcccgg gaatgtcttg ggagcgcgga cctggggatc ggcatagagg ctggtgtgtg
     5221 ggaaagggcc gatggcctgt acgacgtgca gtactgcgca atcatcgatg ccatgggcag
     5281 ggtgagcata gggcacggca tgggcttccg gtatccgcca gccctcgccg ccttagtgcg
     5341 ccaagggttg tccgtcgggg acgcatgcgc ccagctcttc aaggaggggg accagggggc
     5401 gggcatgggg gccataggga tcctgacgaa cggtgtcatt gaccggcagg cgttaacgga
     5461 gcaggccgtg ctggcggcaa tggtgccccg gataagaaag gagctttact agcccagtgc
     5521 cttgtctctc gcctgccgga tgtgagcgag gtaatcgttc tcggtggcgc agttctcagg
     5581 cccgatgcat ccgaaccgat tgtacttcgt gcaataatcg cccccgatgc agtgcgggca
     5641 tgccgcctcc ttatcctgca taaggcattt atccctgatg caagcgaagc ccaggtacag
     5701 cccctggcac ttaccatcgg ggcccagctg cgggcacccg ctcatcccga ccgcctcccc
     5761 cggtggtcca ttgaatcgga acgatcgacg cgcatcgaac tcccatcctc ctctaaagcg
     5821 ataggatatt gattcgtgca tattaaataa ttctcttgaa atttatcgga cccggacccc
     5881 tcggacccgc ttgggcgggc cacaccaagc ggcgggggat cattgaaagg gatgggagca
     5941 tgatgacgaa cgaggcagga acgcgggtgt cgatggaaga cccatagatg acggcctcag
     6001 cctcgagggg gcatggccga cggggatggg tttcggacgg cggtgaggat ggcgctcacg
     6061 gacagccttg ccatggg
//
LOCUS       DAOSYN010000071         6074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_10964_length_6074_cov_4.307028, whole genome shotgun sequence.
ACCESSION   DAOSYN010000071 DAOSYN010000000
VERSION     DAOSYN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10964_length_6074_cov_4.307028"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLJ11_04120"
     CDS             complement(<1..341)
                     /locus_tag="PLJ11_04120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO03889.1"
                     /translation="MSRRAWDLLYRNDRHPWKGGQDVELPLTGTVLELGIGNGKNLSA
                     LPQGARVIGLDFSRPALLSCRERTAVQLVQANILALPFRDGCMDGIAASHVLGHLDGE
                     GRRRAASEIVRV"
     gene            complement(338..895)
                     /locus_tag="PLJ11_04125"
     CDS             complement(338..895)
                     /locus_tag="PLJ11_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO03890.1"
                     /translation="MAHQAQFPASERHRLVDEERQRRQPVEMLLARIRPRKGEAIADL
                     GAGVGYLSLPLAAAGAEVIALDSQQEMLEGLRERGNGSERVQLVKAILPSIPLRDASL
                     DRVVMVNVLHEVEDKAGLAKEIARVLRPTGRMTLVDWQARETGRGPPLHERVPVELAP
                     SIFEGFVLERGYDDGDHYHLELRRE"
     gene            complement(921..2732)
                     /locus_tag="PLJ11_04130"
     CDS             complement(921..2732)
                     /locus_tag="PLJ11_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOO03891.1"
                     /translation="MLLRAEGIGKSFGPREVLKDVDLQIELGDRIGLVGRNGAGKTTL
                     VKVLMGQLKPDVGKVELRTSKIAYLSQFVEVDRSSAVGSAVAVLSEEEAALRRRAEEI
                     EATMTSGGGPETDWNALSAEYAHIHEELARLSSQRTDGKALDILEGLGLEDKAEGRMG
                     DLSGGERAKVMLSRALAQAERAELVFMDEPTSHLDLETVEWLEDQLLKLKGAVMIISH
                     DRYFLNNVATRIVELEAGRLTHYTGNYSVFMEKKALDRERLLQAHERALRERERQERI
                     ADELHVRYKFKSYHRTREKMAERVEVPEPPPEGKDLEVRIYALGKSGKNVLTAKGLEV
                     FRGKRKVLDKVDLELEKGDRLGIFGPNGSGKTTLLKALQGRLPYQGELYVSPGVKIGY
                     YAQEHELLEPELTAEEQMRKALGQQATEARAILARLLLTGKDVERPIATLSGGERARL
                     ALAILLAQHKNLLILDEPTNYLDIPSREAVEEALREYTGTLIVVTHDRYLLDAVCTKV
                     GELRDGKLAVFNGTYSEMKGRQSFSQGIEEAEVYKAVSGFKDWTSGVTYRAGDKVTIA
                     RSERENFKWALETGKLKRVPGTELKKVRRPPAVQGDD"
     gene            complement(2769..3503)
                     /locus_tag="PLJ11_04135"
     CDS             complement(2769..3503)
                     /locus_tag="PLJ11_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOO03892.1"
                     /translation="MDVVFMGTNGWYDTETGNTLSVLVRSKEFNVVLDAGGGLHKLDR
                     YADLSKPTVIMLSHHHLDHVIGLHQLVRFRFQGKVTIVGIMGTHTILHELLRDPYSVP
                     LGLIPYNIEVLEVKEGVHERPFPFHSRLLFHSSPCLGYRLELDDRVIAFCTDTGPCKN
                     TVELARGADLLITESAFRTGESSDLWPHMTPEDALEIKRAAGAKSLVLTHFDAYKYPT
                     LRDREEIEERYPGELRVSKDDMVFKL"
     gene            3600..3746
                     /locus_tag="PLJ11_04140"
     CDS             3600..3746
                     /locus_tag="PLJ11_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03893.1"
                     /translation="MNVEQELLLRQVIEELKVQSQYLFDIRMLLMRSLDAGSKAPNEA
                     PRSR"
     gene            3751..4593
                     /locus_tag="PLJ11_04145"
     CDS             3751..4593
                     /locus_tag="PLJ11_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOO03894.1"
                     /translation="MGQGRLGIIGCPVLEDEIVYLLTSDRDLKDVFVVEDDNSEKLIQ
                     KLRAKGVPIVPIKEGDIAGLPNGAGRSVLVWMKSMGLHEDPGELGREVSRTVMALDGH
                     CSVILLFYGLCGNAFKDMDRLLEPVRTPVVILRDEEGRIVDDCIAVPLGGTDGYLRLL
                     KRYAGVFYMTPAWADNWRTLINKMEITRGTEMGDLDMLRMIFEMTGYNRVLMIDTGLG
                     DREVLRTRTHEFAQEFGFRELELEPGFVSTAVSDAAYQRCIELMEHGKKGRGEEGPKS
                     VPSS"
     gene            complement(4725..4847)
                     /locus_tag="PLJ11_04150"
     CDS             complement(4725..4847)
                     /locus_tag="PLJ11_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03895.1"
                     /translation="MHDVVCSDCGAKTQVPFKPTEGRPVYCRDCFQKHKPKDRF"
     gene            5275..5760
                     /locus_tag="PLJ11_04155"
     CDS             5275..5760
                     /locus_tag="PLJ11_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOO03896.1"
                     /translation="MKGVVCYDSVYGSTKQVAEAIAEQLRAEGHEAVLVSVKDRPRPK
                     LEGDIMFIGSPTRWFTMTKDAKDFVKDLRAMGWKDQPIVMFDTMLGVPEDLGERTKGE
                     WGRKGAAAKMRDMAKADGLSVRDEVLHIGVTGMKGPLTATGAQEAKGYASQVLASMGK
                     R"
BASE COUNT         1097 a         1900 c         1894 g         1183 t
ORIGIN      
        1 acccgtacga tctcggaggc cgccctgcgc ctgccctcgc cgtccaaatg accgaggacg
       61 tgcgaggccg ctatgccgtc catgcacccg tcccggaacg ggagcgcaag tatgttcgcc
      121 tggaccagct ggaccgcggt cctttccctg caggacagca gcgccggccg ggagaagtcc
      181 aggccgatca ccctcgcccc ctggggaagg gcggagaggt tcttcccatt gccgatgccc
      241 agctccagga ccgtgcccgt gagcgggagt tcgacgtcct ggcccccctt ccaggggtgc
      301 cgatcgttcc ggtagaggag gtcccacgcc cttctgctca ttccctgcgc agctccaggt
      361 ggtagtggtc cccgtcatcg tatccgcgct cgagcacgaa gccctcgaag atggacgggg
      421 cgagttccac cggcaccctc tcgtgcaggg gcggccccct tccggtctcc cttgcctgcc
      481 agtccaccag ggtcatcctg ccggtggggc ggagcacccg cgcgatctcc ttcgccagcc
      541 ctgccttgtc ctccacctcg tggaggacgt tcaccatgac aacgcggtcg agcgaggcgt
      601 cccgcagcgg gatgctcggt aggatagcct taaccagctg gaccctctcg gagccgttgc
      661 ccctctccct cagcccctcg agcatctcct gctgggagtc aagcgcgata acctcggctc
      721 ccgcggctgc cagaggaagg gagagatagc cgacgcccgc ccctaggtcg gcgatcgcct
      781 cgcccttccg ggggcggatg cgggcgagca acatctccac cggctgtcgg cgctgccgct
      841 cctcgtcgac caggcggtgc cgctcagagg ccgggaactg ggcctgatgc gccatgcctc
      901 accatcctcg gggcggtatt tcaatcatcc ccctgcaccg ctggggggcg gcgcaccttc
      961 ttgagctcgg tcccggggac ccgtttcagc ttgccggtct cgagggccca tttgaagttc
     1021 tccctctccg acctggcgat ggtgaccttg tcccctgccc tgtaggtcac cccgctcgtc
     1081 cagtccttga acccggagac ggccttgtac acctcggcct cctcgatgcc ctgggaaaag
     1141 ctctgtctcc ccttcatctc cgaatatgtg ccgttgaaga cggcgagctt gccgtccctg
     1201 agctccccga ccttggtgca gaccgcgtcc aacaggtatc ggtcatgggt gacgacgatg
     1261 agcgtgccgg tatactcccg cagcgcctcc tccaccgcct ccctgctcgg gatgtccaga
     1321 tagttcgtgg gctcatcgag gatgagcagg ttcttgtgct gggcgagcag gatcgccagg
     1381 gccaggcgcg ccctttcccc gcccgagagg gtggctatgg gcctttcgac gtccttgccg
     1441 gtcaggagca gcctcgcgag gatggccctg gcctcggtgg cctgctgtcc caatgccttc
     1501 ctcatctgct cctcggccgt cagctccggc tctaagagtt cgtgctcctg cgcgtagtac
     1561 ccgatcttaa cccccgggga gacgtaaagc tccccctggt atgggagcct gccctgcagc
     1621 gccttcagca aggtcgtctt cccgcttccg ttcggcccga agatgcccag cctgtccccc
     1681 ttctcaagct ccaggtccac cttgtccaga accttgcgct ttccgcggaa cacctccaac
     1741 cccttggcgg tgaggacgtt cttgcccgac ttaccaaggg cgtagatccg cacttccagg
     1801 tccttcccct cgggcggagg ctccgggacc tccaccctct cggccatctt ctccctggtc
     1861 ctgtgatagg atttgaactt gtacctgaca tgcagctcat cggcgatgcg ctcctgccgc
     1921 tccctctccc gcagggccct ctcatgggcc tggaggagcc tctcccggtc cagggccttc
     1981 ttctccatga acacggagta gttgccggta taatgggtca gccttccggc ctccagctcg
     2041 acgatgcggg tcgcgacatt gttcaggaag tagcggtcgt gggagatgat cataaccgcg
     2101 cccttgagct tgaggagctg gtcctccagc cattccaccg tctcgaggtc cagatggctg
     2161 gtgggctcgt ccatgaagac cagctcggcc ctctcggcct gggcgagcgc ccgggagagc
     2221 atgaccttgg cgcgctcccc tccgctgagg tcccccatcc tgccttcggc cttgtcctca
     2281 agcccgagcc cctccaggat gtccaatgcc ttgccatcgg tccgctggga agataggcgg
     2341 gccagttcct cgtggatatg cgcgtactcc gcggaaaggg cgttccagtc cgtctcaggc
     2401 cctccgcccg acgtcatggt cgcctcgatc tcctctgccc ttctccgcag ggcggcctcc
     2461 tcctcgctca ggacggccac ggcggagccg acggccgagg agcggtccac ctcgacgaac
     2521 tgcgagaggt aggcgatctt gctggtccgc agctccacct tgcccacgtc cggcttgagc
     2581 tgccccatga gcaccttgac gagggtggtc ttgccggccc cgttccggcc gaccagcccg
     2641 atgcggtccc ccagctcgat ctggaggtcc acatccttca ggacctcccg ggggccgaag
     2701 ctcttgccta tcccctccgc gcgcaggagc atgccaaggc aatcgtgccc cggcctttat
     2761 gcctttccct agagcttgaa caccatatcg tccttggaca ccctcagctc gcctgggtag
     2821 cgctcctcga tctcctcgcg gtcccgcagg gtggggtact tgtacgcatc gaagtgcgtg
     2881 aggaccaggc tcttggcgcc ggccgcccgc ttgatctcca gggcatcctc cggcgtcatg
     2941 tgcggccaca ggtcgctgct ctccccggtc cggaaggcgc tctcggtgat gaggaggtcc
     3001 gcgcccctgg ccagctccac ggtgttcttg catggcccag tatccgtgca gaaggcgatg
     3061 accctgtcgt ccagctcaag acggtacccg aggcaggggg aggaatggaa gagcaggcgg
     3121 gaatggaagg ggaaaggcct ctcgtgcacc ccctccttga cctccagcac ctcgatgttg
     3181 tagggaatga gccccaaagg caccgagtag gggtcgcgca ggagctcatg caggatggtg
     3241 tgggtgccca ttatcccgac gatggtgacc ttcccctgaa agcggaagcg gaccagctga
     3301 tgaaggccga tgacgtggtc caggtgatgg tgggagagca tgatgacggt cggcttcgac
     3361 aggtcggcgt agcggtccaa cttgtgcaga ccgcctccgg cgtccagcac cacgttgaac
     3421 tcctttgagc gcaccaggac gctcagcgtg ttccctgttt ccgtatcgta ccagccgttc
     3481 gttcccatga acactacgtc cactcgacca ccgaggatgc tatggcgctc gcccccataa
     3541 ataagcattc ggcccgggga gggcaatcca ttattaccat ggatgccctt ccccattcga
     3601 tgaacgtcga gcaagaactg ctcctgaggc aggtcatcga ggagctcaag gtccagagcc
     3661 agtacctatt tgacatccga atgctcctca tgaggtcatt ggatgccggg agcaaggccc
     3721 ccaacgaagc gccacggtca aggtgagcca atgggacaag ggagattggg catcattggc
     3781 tgccccgtgc tggaggatga gatagtgtac cttctgacca gtgaccggga tctgaaggac
     3841 gtgttcgtgg tcgaggatga caactcggag aagctcatcc aaaagctgcg cgccaagggg
     3901 gttcccatcg tccctataaa ggaaggggac attgccggcc tccccaatgg cgctggtcgc
     3961 tccgtcttgg tatggatgaa gtccatgggg ctgcatgagg accctgggga gcttgggagg
     4021 gaagtctcta ggacggtcat ggcgctggac gggcattgct cggtcatcct gctgttctat
     4081 gggctatgtg gcaatgcatt caaggacatg gacagactgt tggaacccgt taggacaccc
     4141 gtggtgatcc tgagggacga ggaggggcgg atcgtcgacg attgcatcgc ggtgcccctg
     4201 ggcggcacgg atggctacct gcggctcctg aagcggtatg cgggggtgtt ctacatgacc
     4261 ccggcctggg cggacaattg gaggacgctc atcaacaaga tggagatcac caggggcacg
     4321 gagatgggag acctggacat gctgaggatg atctttgaga tgaccggata taaccgggtt
     4381 ttgatgatag acaccggcct aggggaccga gaggtcctgc gcacccggac gcacgagttc
     4441 gcccaggagt tcggcttccg agagttagag ctggagccgg ggttcgtatc caccgccgta
     4501 agcgatgccg cctatcagag atgcatcgaa ctcatggagc acggcaagaa ggggcgcgga
     4561 gaggaaggac cgaagtccgt tccttcctcc tgagcggcgg gaccagcgcc tcatacgtcc
     4621 cagatgctgc gccaccatgg gatgagggcg ccgatgatgc gaaatgatga gaatacaatg
     4681 agaatgaaaa aaggaaagtg gtttgtcgaa gttgccttct gagtttagaa cctgtccttc
     4741 ggcttgtgct tctggaagca gtccctgcag tagacaggcc tgccctcagt cggcttgaat
     4801 ggaacctggg tcttagctcc acagtcggag caaactacat cgtgcatttc gcggggcccg
     4861 tcatttctgg ggcggttgta tccgcccgac ctctgtcgtc cgtacattac tactacctat
     4921 tgttgttggc aaagatcagc tgacccttgc aacggataat gcgaagcaat tactactatt
     4981 tatattaaac acccggggac agcggaaatg atagggtggt gccgtgcatc gtttgggcgc
     5041 tgatggcacg atgcggccat caatgtggat tgaaaatggc cttccaccgg tcttaccact
     5101 gccacgtcat ccataggaac gcccccggtt ctccgaacgg tgcgtgtgtt cgagagggat
     5161 atgaggaaag atccggacgc catttcccga accatcgtgt tgagcgggcc atcctcaaga
     5221 ccaagattga tctgctagct caggctatta gataggaggg agggaggcat ttcgatgaaa
     5281 ggggtcgttt gctacgatag tgtctatggg tccaccaagc aggtggccga ggccatagcc
     5341 gagcagttga gggcggaggg gcatgaggcg gtcctcgtca gcgtgaagga caggccgaga
     5401 ccgaagttgg aaggggacat catgttcata gggtccccga cccgctggtt cacgatgacc
     5461 aaggatgcca aggacttcgt gaaggacctg agggccatgg gctggaagga ccagcccatc
     5521 gtcatgttcg acaccatgct gggagtgccg gaggacttgg gggagaggac caagggggaa
     5581 tgggggcgca agggggcggc cgcgaagatg cgggacatgg ccaaagcgga cggactctcc
     5641 gttcgggatg aggtcctgca tatcggggtc accgggatga aggggcctct tacggccacg
     5701 ggcgctcagg aggccaaggg atacgcctcc caggtactgg cgtccatggg gaagaggtga
     5761 gccaaaggag cgggggatcg cataggggaa taggcatcgg ggcccatgac gggacccacc
     5821 gtcccacgat cgctcgcccc tccatcggcc tcgtctcgaa gggctctccc gatcatggcc
     5881 gcaacgcttc tttttcctag caccctcata cgcgatttga aggaccgcaa ggaagtgacc
     5941 aatggctaca cgaaacggag cgggccatgg gaccaacggt ctgggtattg gttcgtcccc
     6001 gatggatcga gagaagtggt ggacactggg ggatttgaac ccccggcctc cgccttgcga
     6061 aggcggcgat cttt
//
LOCUS       DAOSYN010000072         6035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_11051_length_6035_cov_4.303846, whole genome shotgun sequence.
ACCESSION   DAOSYN010000072 DAOSYN010000000
VERSION     DAOSYN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11051_length_6035_cov_4.303846"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="PLJ11_04160"
     CDS             complement(<1..1485)
                     /locus_tag="PLJ11_04160"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOO03897.1"
                     /translation="MLFRELVDTYERLEATSSRLEMTEILAEAFRRFDPAEIRMAVYL
                     TQGQLYPDFVPLKLGMADKLALKAIAFASGAGEPLVQELMRKEGDPGLVAYIVFQSKK
                     QTTLFSEPLTLKRAYETLEAIARAEGGGSQEHKVKLLAKLLSDASPSEAKFLSRVITG
                     RMRLGVSTMTIVDALAVAFASKEDRDAVERAFNVSSDLGLVAETLALHGLEGVKGIRV
                     QVNRPLRAMLAERMSSPEEILERMGGRCAFEYKYDGVRLQAHICDGEVRLFSRKLDEL
                     TAQFPDVVAGLKRAVHARSAIVEGECVPVDTNTGEFLPFQEVSHRRGRKYGVHEAVED
                     YPVKLFLFDCILKDGEDLTDKPFLERRAALTSIVVPDETVALSEIRLLSSPAEVQAFF
                     QEAIRDGCEGLMAKSVAENSVYRAGNRGFLWIKYKKEYRSEMTDTVDLVVVGAFAGRG
                     KRRGLYGALLMATYDPATDTFQTVSKLGSGFDDAALAALPEMLAN"
     gene            complement(1527..3092)
                     /locus_tag="PLJ11_04165"
     CDS             complement(1527..3092)
                     /locus_tag="PLJ11_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO03898.1"
                     /translation="MYYEHPLIKRESVQRRDYQVELARQASLTSTLLVLPTGLGKTVV
                     AAMVIAHVLHAKGGKVLFLAPTRPLVEQHYRSLQGMIEGKRIGMMTGEVDPLERELTF
                     LESDIVVSTPQVVSNDLRQGRMDLRDVRLLIFDEAHRAVGNYAYVDVAKAYLDHDGLV
                     LGMTASPGSDKAKIEEVCRNLNIKAIEVRTERDPDVAKYVQAISMDWVEVELPPEMKA
                     LAQSIRSLYEQYLRELVDLQVVDKDRVTNKRYLLEMAETWQARLRAGERSRALYKALS
                     VQAKAVKVEHALDLVETQGMSALRAYLQRLKEEAAMEDGSKAAREIVSSERFQRICEE
                     AEAVKAEHPKLSRVMGVVARQLADSPSSRVLVFTHYRDTCDLVASKLGNVEGARVAKL
                     VGQSDRNGERGLRQREQVGVLQRFRDGELNVLVATSVGEEGLDVASTDLVVFYEPVPS
                     EIRTIQRRGRTGRSRAGRVVILITKGTRDEAYYYSSLNKERAMRRRLETLKEELGDGG
                     RPKERGQLTLDGF"
     gene            complement(3140..3463)
                     /locus_tag="PLJ11_04170"
     CDS             complement(3140..3463)
                     /locus_tag="PLJ11_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOO03899.1"
                     /translation="MVTVTEEASKYITDLIAKNNKSGYGIRIYLAGMGCSGAQFGMAF
                     QKDPKEGDIEEKLDGFSVYYDEETKEVLDACTVDFVETPYGSGLVVNNPNAVSGCGSC
                     GGGCH"
     gene            3643..4065
                     /locus_tag="PLJ11_04175"
     CDS             3643..4065
                     /locus_tag="PLJ11_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO03900.1"
                     /translation="MRRADLPALVAMSRENMAAIILSSWGEEWKDETLLDMLLDQRVD
                     TTVLDGEDGIEAYYCVEDMDDCIFISSIQVRRGCQGRGLGGRMLRMIEDQAKSDGKAW
                     VELCVQSTNERAKEFYHYHGYDLMYANGNNIVMRKRLG"
     gene            4116..4571
                     /locus_tag="PLJ11_04180"
     CDS             4116..4571
                     /locus_tag="PLJ11_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOO03901.1"
                     /translation="MAPRSIPLCITLLLVALLAVPSVQAVPEAPEALRATAGNGYADL
                     SWLPVDGAERYLLYRGDMDGMAMIANLSAPFTAFHDAQVEEGESYLYYVTAVVGGEEG
                     APSNTVAVTVPEEERDFLLPVLALVISVIAIQVCVVMLLYQFKSNFRLK"
     gene            complement(4585..5553)
                     /locus_tag="PLJ11_04185"
     CDS             complement(4585..5553)
                     /locus_tag="PLJ11_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOO03902.1"
                     /translation="MGSGCFSRDARDKHIITRGNVDGIVSAAIFLSRFPSAKVTFVTS
                     PVAGAKALTLDTASEQIYLVDIASVPELVEAVRAKRGRRSLILVDHHPTALAVACTAL
                     VREGVSAANVLFHHLGQEARLRKLVALADLIEYMPTALLDEEMRRFGRQRVDQEAMVL
                     DFAWRLKIDDDHFRYLAAKQLAQGVWPSQVDAVRGRYIQVVNENRWPRALAKVDGALK
                     VRGPLGILDEMDRTRGLYGFGTRALVEVAHRRGCGYAVMINPRGRYASVSVRGMAQES
                     VDLGRFVEDYTKRNGVDGGGHPRAAGARIPTGGGEKFLDALLEEVS"
     gene            5718..>6035
                     /locus_tag="PLJ11_04190"
     CDS             5718..>6035
                     /locus_tag="PLJ11_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03903.1"
                     /translation="MSMTTDGLYSYAEGKDEGRGELQRCFAKVGAEHGFTEVEARWEP
                     YKEFKATWRRCASSAELRITDYMMASGAELLTDLARGIFGRMGRGGQREIYSERLRQH
                     FLSP"
BASE COUNT         1019 a         2067 c         1871 g         1078 t
ORIGIN      
        1 gttcgcgagc atctcgggca atgccgccag ggcagcgtca tcgaagcccg agcccagctt
       61 ggatacggtc tggaaggtgt ccgtggccgg gtcgtacgtc gccatgagca gcgccccgta
      121 gagcccgcgg cgcttgccgc ggccggcgaa ggcccccacg accaccagat ccaccgtgtc
      181 ggtcatctcc gaacggtact ccttcttgta cttgatccac aggaagccgc ggttgccggc
      241 ccggtagacc gaattttccg ccacggactt ggccatcaac ccctcgcacc cgtccctgat
      301 ggcctcctgg aagaacgcct gcacctcggc gggcgaggac agcagccgga tctccgacag
      361 ggcgaccgtc tcgtccggaa cgacgatgga ggtcagcgcc gcacgccgct ccaggaaggg
      421 cttgtcggtg aggtcctccc cgtccttgag gatgcagtcg aacaggaaca gcttgacggg
      481 gtagtcctcg acggcctcgt gcacgccgta cttcctcccg cgccggtggg agacctcctg
      541 gaacggcagg aactcgccgg tgttcgtatc gaccggcacg cattccccct cgactatggc
      601 gctccgggcg tgcacggccc tcttcaggcc ggcgacgacg tccgggaact gggccgtgag
      661 ctcgtccagt ttgcgggaga acagtcggac ctccccgtcg caaatgtggg cctgcagccg
      721 caccccgtcg tacttgtact cgaaggcgca cctgcccccc atcctctcca ggatctcctc
      781 gggcgaggac atgcgctccg ccagcatggc cctcagcggt cggttcacct gcacgcggat
      841 ccccttcacc ccttccaggc cgtgcagcgc cagggtctcg gccaccaggc cgaggtccga
      901 ggagacgttg aacgcccgtt ccaccgcatc gcggtcctcc ttgctggcga aggccacggc
      961 cagggcgtcc actatggtca tcgtcgagac cccgaggcgc atccgcccgg ttatcaccct
     1021 ggaaaggaac ttcgcctccg agggggaggc gtcgctgagc agcttggcga gcagcttgac
     1081 cttgtgctcc tgggagccgc cgccctcggc ccgggcgatg gcctccagcg tctcgtacgc
     1141 ccttttcagt gtcaagggct cggagaaaag ggtggtctgc ttcttgctct ggaagacgat
     1201 gtaggcgacc aggcccggat ccccctcctt gcgcatgagc tcctggacca ggggctcccc
     1261 ggcccccgag gcgaaggcga tcgccttcaa ggccaatttg tccgccatgc ccagcttcag
     1321 cgggacgaag tccgggtaca gctgtccctg ggtaaggtag accgccatcc ggatctcggc
     1381 ggggtcgaac cggcggaagg cctcggccag gatctcggtc atctccagcc tcgacgaggt
     1441 ggcctccaac cgttcatagg tgtcgaccag ttcccggaac agcatgcctg gaccatcgac
     1501 tcgcgattat ttaaactgct gaccgcctag aacccgtcga gggtgagctg tccccgctcc
     1561 ttcggccgcc ccccgtcgcc cagctcctcc ttcagcgtct ccaggcgccg gcgcatcgcc
     1621 ctctccttgt taagggacga gtaatagtac gcctcgtccc tggtcccctt ggtgatcagg
     1681 atgaccaccc tcccggcgcg cgagcgcccg gtgcggcccc gccgctggat cgtgcggatc
     1741 tctgacggca ccggctcata gaagaccacc aggtcggtgc tggccacgtc cagtccctcc
     1801 tccccgaccg aggtggcgac gagcacgttc agctccccgt cccggaaccg ctgcagcacc
     1861 cccacctgct ccctctggcg cagccccctc tccccattgc ggtcggactg ccccaccagc
     1921 ttcgccaccc tggcgccctc aacgttcccc aacttggagg ccaccaggtc gcaggtgtcc
     1981 cggtagtgcg tgaacaccag gacccgggag ctggggctgt ccgccagctg cctggccacc
     2041 acgcccatca ccctcgagag cttggggtgc tcggccttca ccgcctccgc ctcctcgcat
     2101 atcctctgga agcgctccga ggacacgatc tcccgggcgg ccttggagcc atcctccatg
     2161 gccgcctcct ccttcaaccg ctgaagatag gcccgcaggg cggacatgcc ctgcgtctcc
     2221 accaggtcga gggcgtgctc gaccttcacc gccttggcct ggacgctgag cgccttgtac
     2281 agcgccctgc tcctctcccc cgcccggagg cgggcctgcc aggtctcggc catctccagg
     2341 aggtaccgct tgttcgtcac ccggtccttg tccaccacct gcaggtccac cagctcccgg
     2401 aggtactgct cgtagagcga ccggatgctc tgtgccagcg ccttcatctc cgggggcagc
     2461 tccacctcca cccagtccat gctgatggcc tgcacgtact tggccacgtc gggatcccgt
     2521 tccgtgcgta cctcgatcgc ctttatgttc aggttgcggc agacctcctc gatcttggcc
     2581 ttgtcgcttc ccggggaggc ggtcatcccc agtaccagac cgtcatggtc aaggtacgcc
     2641 ttggccacgt ccacgtaggc gtagttcccg accgcccggt gcgcctcgtc gaagatgagc
     2701 aggcgtacgt ccctcagatc catgcggccc tgccggaggt cgttcgagac gacctgcggc
     2761 gtcgagacca ctatgtcgct ctccaggaac gtaagctccc gctccagggg gtccacctcg
     2821 ccggtcatca tgccgatgcg cttcccctcg atcatgccct gcaatgagcg atagtgctgc
     2881 tcgaccaagg gccgggtggg cgccagaaag aggaccttcc cccccttggc gtgcagcaca
     2941 tgggcgatga ccatggccgc caccacggtc ttcccgaggc cggtggggag caccagcagg
     3001 gtggaggtga gggaggcctg gcgggcgagc tccacctggt aatccctacg ctggacgctt
     3061 tcccgcttta tcagggggtg ctcataatac acgatgggta atcgctatcg gactaaaaag
     3121 gaaagggatg ggaagaggtt cagtggcagc cgccgccaca ggagccgcag ccgctgacgg
     3181 cgttggggtt gttgaccacg aggccggaac cgtagggggt ctccacgaag tccacggtgc
     3241 aagcgtccag gacctccttg gtctcctcgt cgtagtagac ggagaagccg tccagcttct
     3301 cctcgatgtc cccttccttg gggtccttct ggaacgccat gccgaactga gccccagagc
     3361 agcccatgcc ggccaggtag atcctgatac cgtacccgct cttgttgttc ttggctatca
     3421 ggtccgtgat gtacttgcta gcttcctcgg tgacagtgac catcttttcc cgtctcctga
     3481 gggtttcaaa acaagaggat gtatataaac aatcgtatgg aagccccatg ccatggcctc
     3541 gcaggcccgg gctggagagc ggcgagcggc cggtcgcacg ggacgggagc cactatcctt
     3601 attctagggg cttggcatac tccctcgatg cgatgccgtc gtatgcggag ggccgacctc
     3661 cccgcgctcg tggccatgag cagggagaac atggccgcaa tcatcctttc ctcctggggc
     3721 gaggaatgga aggacgagac gctcctggac atgctcctcg accagagggt ggacaccacg
     3781 gtgctcgatg gggaggacgg gatcgaggcg tactactgcg tggaggacat ggacgactgc
     3841 atcttcatct cctccatcca ggtccggagg ggatgccagg gaagggggct gggggggcgg
     3901 atgctccgaa tgatcgagga ccaggccaag agcgatggca aggcctgggt ggagctctgc
     3961 gtccagtcga ccaacgagcg ggccaaggag ttctatcact accatggcta tgacctgatg
     4021 tacgccaacg gcaacaatat cgtcatgcgt aagcggctgg gctgatgcca aggttttaat
     4081 cgtcgcccga ggatctggaa aggaggtagt ccgccatggc accccgttcc atcccgctgt
     4141 gcatcacgct gctcctcgtc gcgctactgg ccgtcccgtc cgtgcaggcc gttccagagg
     4201 cgccggaggc cctgcgggcc accgcgggga acggctacgc ggacctcagc tggcttccgg
     4261 tggacggcgc ggagcggtac ctcctgtacc gcggggacat ggacgggatg gcgatgatcg
     4321 cgaacctgtc cgctccgttc accgccttcc acgatgccca ggtggaggag ggggagagct
     4381 acctctatta cgtcaccgcc gtcgtgggcg gggaggaggg cgccccgagc aacacggtcg
     4441 ccgtgaccgt ccccgaggag gaacgggact ttctccttcc ggtcctggca ttggtgatct
     4501 ccgtgatagc catccaggta tgcgtggtca tgctcctcta tcaattcaaa tcgaacttcc
     4561 ggctcaagtg acgccctgct tcggtcatga cacctcttcc agaagggcgt ccaggaactt
     4621 ctctccgccg cccgtcggga tcctggcgcc ggcggccctc ggatgcccgc ccccatcgac
     4681 gccgttcctc ttggtgtaat cctccacgaa ccggccgagg tccacgctct cctgggccat
     4741 gccccggacg gagaccgagg cgtacctgcc gcgggggttg atcatcaccg catagccgca
     4801 ccctcggcgg tgagccacct cgacgagggc cctcgtcccg aagccgtaga ggccccgggt
     4861 acggtccatc tcgtccagga tgcccaaggg cccgcgcacc ttcagggcgc cgtcgacctt
     4921 ggccaacgcc ctgggccacc tgttctcgtt gaccacctgg atatagcgcc ccctcaccgc
     4981 gtccacctgg gagggccaga cgccctgggc gagctgcttc gccgccaggt accggaaatg
     5041 atcatcatcg atcttaaggc gccaggcgaa gtcgaggacc atcgcctctt ggtccaccct
     5101 ctgtctcccg aagcggcgca tctcctcgtc cagcagcgcg gtcggcatgt actcgatcag
     5161 gtcggccaat gccaccagct ttcgcaggcg cgcctcctga ccaagatgat ggaacagcac
     5221 gttcgccgcg ctcaccccct cccgcacaag ggcggtgcac gccaccgcca aggccgtggg
     5281 gtgatggtcc acaaggatga gggagcggcg gccccgcttc gcccgcaccg cctcgaccag
     5341 ctctggcacc gaggcgatgt ccaccagata gatctgctcg gatgcggtgt ccagcgtcag
     5401 cgccttggcc ccggcgacag gggaggtgac gaacgtcacc ttggccgaag ggaacctgct
     5461 taggaagatg gccgcggaca cgatgccgtc aacgttgccc cgggtgatga tgtgcttgtc
     5521 tcgggcgtcc ctcgaaaagc agccgcttcc catccgctgt aacacattga tcccagacta
     5581 gataatccta ttgcctggcc tcccttggcc ggcctggaag cgggccaagg cccttatcct
     5641 cggcattcat taccgtaccg agcgtagcgt gcagctactc ccacccgtta tgggcatcgc
     5701 caagggtcgg agggcagatg agcatgacga cggacggtct gtactcgtat gccgagggaa
     5761 aggacgaagg gaggggtgag cttcagcgct gcttcgccaa ggtgggggcg gagcacgggt
     5821 ttaccgaggt ggaagcgaga tgggagccct acaaggagtt caaggcaacg tggaggcgat
     5881 gcgcgtccag cgcggagctg cgcatcacgg actatatgat ggcctccggg gccgagctgc
     5941 taaccgacct tgcccggggc atcttcggcc ggatgggccg gggaggccag agggaaatat
     6001 actcggagcg cctgagacag cacttcctga gcccg
//
LOCUS       DAOSYN010000073         5996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_11139_length_5996_cov_3.613701, whole genome shotgun sequence.
ACCESSION   DAOSYN010000073 DAOSYN010000000
VERSION     DAOSYN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11139_length_5996_cov_3.613701"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLJ11_04195"
     CDS             complement(<1..585)
                     /locus_tag="PLJ11_04195"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOO03904.1"
                     /translation="MKQCVAVLAPAEGWEPLCQRFKELEFLFVPPLTCVPMDSAAFSY
                     FLSDLAVGRFDAVVMTCPMAVRAVIDIANSRGMLNRFISSLARTHLVVIGDRTAESAR
                     WNGLRVGSVSPEVSNDALVKHVQALPFRGSIALLRSDRGTKALPRALVECGWTVTEVP
                     VYSPRLAQSEDMELLLDRMEKREVAALAFPTPLQT"
     gene            725..1372
                     /locus_tag="PLJ11_04200"
     CDS             725..1372
                     /locus_tag="PLJ11_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO03905.1"
                     /translation="MRSVTLRLKMLDDHICCNCLEGMEERGLSIKVLRCVPMGERHAH
                     SLLRIDGDPEICEELLANPPELERDGMRIEMARSGNGTISLLVINEGCRLSHLISESG
                     CFLELAIPAGDDGMIFHLLGRDAEAITDFVRMAKEEGYAIEVISTYEKREWGGLTFRQ
                     ERDLRIAFDLGYFDIPSRITLDELASKVGCSKSTLNVILRRAERKIISDYLKGAK"
     gene            complement(1460..2506)
                     /locus_tag="PLJ11_04205"
     CDS             complement(1460..2506)
                     /locus_tag="PLJ11_04205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004889.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtaA/CmuA family methyltransferase"
                     /protein_id="HOO03906.1"
                     /translation="MKDDKGEMRPRERFFRAMACKEVDRPPVCGMTTTATVQLMDKVG
                     AAWPEAHTDAELMSRIALGAPDFFGFESVRVPYCLTYEAEALGCKVFLGKKNSTPMVK
                     SHPYRDDPDAELVLPKPKEIMEMARNKVVIEAAKIIKEKAGKELPTLLGVTGPFTIAG
                     HLVGTENLLYWIVTDPEKVHKFVKFAAEYEREWLKIVETLGIDVIQMSEPSASYDMMS
                     PEMFEEYALPYIKRTYEPLENTKKVLHICGNTTVILPLMMRTGADGLSIEEKVDPYKA
                     VEIVDGKAALVGNVGVVKPLLQGTPEEVRRDALRSADAGFNIISAGCGMSALIDVENV
                     RAMTSAIKSYKKKR"
     gene            2851..4158
                     /locus_tag="PLJ11_04210"
     CDS             2851..4158
                     /locus_tag="PLJ11_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO03907.1"
                     /translation="MVVNEELKKKMLRYRWVIFGVLALAYFFVYFHRVSVAVVSSDIK
                     AQFGVGASSIALLSSAYFYTYTIMQLPSGLLTDSWGIKKTAGVFTFILAIGAVLCGVA
                     TSFEMILLGRLLIGLGAAMVYIPVMKVLALWFRKYEFASVNGILLAVGSIGSLSAATP
                     LALMSDALGWQEVFLLLGMVSVVTAILIFILVKDHPKDMGFPTIPEIEAFEKGEPLPE
                     TSAQEAKAEEKVPMGKALKDVFGSGLKFWPLAIWFFFMYGSIMVYQGLWGSQYFRDIL
                     GWDKGTYALTITFIGIGMIFGSPLAGTISDKIHSRKKVLVIGTVCYTAVWAALLLLMG
                     ECESELVYHALHFLFGFFGGFFVVCYAMIKEHFPTAIAGTSTGALNLFPFAGGAILQL
                     LSGYMILDYSLSEYRSVWLFMLVGMVIACACIFLAKERTKEGA"
     gene            complement(4444..5226)
                     /locus_tag="PLJ11_04215"
     CDS             complement(4444..5226)
                     /locus_tag="PLJ11_04215"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOO03908.1"
                     /translation="MRKQVVILGPPEQWAPLLRDHRQLIDITFAQPLRAVPRDDSVFS
                     LFLVDLLSGRYSMLIATCPMAIEAAVAMARKRRMLDRLREAVVRTELVVIGERTAQGA
                     ARNGLTVSSIAPEATTDALIEHVNRSPHRGAVALLRSDQGSERMVRGLQAAGWEVEDV
                     PVYTLLLDEGEGMQALLDRIEDGEVDALVLPTPAHAQAFMLQLQERCGDDAPRMLEGI
                     TIAAMGRETEEWLEAFGVKVDVRPERAEAKTLLSALTDRLGV"
     gene            complement(5280..5447)
                     /locus_tag="PLJ11_04220"
     CDS             complement(5280..5447)
                     /locus_tag="PLJ11_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03909.1"
                     /translation="MKPRATAAALLAKGIDCRIALCEAMGYPEERIVLGDPSHPPSPR
                     GQMFAVVVGRW"
     gene            5470..5667
                     /locus_tag="PLJ11_04225"
     CDS             5470..5667
                     /locus_tag="PLJ11_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03910.1"
                     /translation="MAEAILNNVSGGRHRRKATEAVERLAKVPVLGAVERGSPLPERH
                     LRLVPAEEQGDLKALRAGLRP"
     gene            5664..>5996
                     /locus_tag="PLJ11_04230"
     CDS             5664..>5996
                     /locus_tag="PLJ11_04230"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenobyrinic acid a,c-diamide synthase
                     (glutamine-hydrolyzing)"
                     /protein_id="HOO03911.1"
                     /translation="MIEEVDLEMLLEIGEKAGPMEPRDAPVLPEGSADIRVAVPRDAS
                     FCFSYPGNLEALQRAGGRLTFFRPTEGEPLPEADLYYLGGGYPEAHLEELASNRDFLE
                     GLRTAAEDG"
BASE COUNT         1069 a         1857 c         1930 g         1140 t
ORIGIN      
        1 ggtctggagc ggggtgggga aggccagggc ggctacctcc cgtttctcca tgcggtcgag
       61 cagcagctcc atgtcctcgg actgggcgag gcggggggag tagacgggga cctcggtcac
      121 ggtccacccg cattcgacca gggcccgggg gagcgccttg gtgcccctgt cggagcgcag
      181 cagggcgatg cttccgcgga acgggagggc ctgcacatgc ttcacgagcg cgtcgttgga
      241 cacctccggg gaaacggagc ctactctgag gccgttccac ctggcgctct ccgcggtgcg
      301 gtccccgatg acgaccagat gcgtccttgc cagggaggag atgaaccggt tcagcatccc
      361 cctgctgttc gcaatgtcga tgaccgcccg gaccgccatg gggcaggtca tcaccaccgc
      421 atcgaacctg cccaccgcga ggtcggaaag gaaataggaa aaggcggcgc tatccattgg
      481 cacgcatgtt agaggaggga cgaacaggaa ctccagttcc ttgaacctct ggcagagcgg
      541 ctcccagccc tccgcagggg caagcaccgc cacgcattgc ttcatctgaa ggggaattgc
      601 ccacgtatta tgaacctttt tctaacgatg cggggatgca tatatatgat atatataata
      661 aatacaatgt ttataaaagg acaatccctg ccctttaata tcccctatac cgttttacgg
      721 aaccatgcgg tcggtcaccc tgaggctcaa aatgctggac gaccacatat gctgcaactg
      781 cctggaaggc atggaggagc gggggctgtc gatcaaggtc ctgcgctgcg tcccgatggg
      841 cgagcggcat gcgcacagcc tcctgcggat cgacggggac ccggagatat gcgaggagct
      901 tttagcgaac cctccggagc ttgagaggga tggtatgcgg atagagatgg cccgctcggg
      961 gaacgggacc atctccctgc tggtgatcaa cgaggggtgt cgcctcagtc acctgatctc
     1021 cgaatcgggg tgcttcctgg aactggccat accggcgggg gacgacggga tgatcttcca
     1081 cctgctgggc agggacgcgg aggcgatcac cgacttcgtg cgcatggcca aggaggaggg
     1141 gtatgccatc gaggtgatca gcacctacga gaagcgggaa tggggcgggc tgaccttccg
     1201 gcaggagcgg gacctgcgca tcgcgttcga cctcggctac ttcgacattc caagccggat
     1261 caccctggac gaactggcct ccaaggtagg ctgctccaag tccaccttga acgtgatcct
     1321 gcgcagggcg gagaggaaga taatctccga ttatctgaaa ggggctaaat gaaaacaggg
     1381 gccgggcggg gagaggtcat gggcacggga ccgatggcag agcggtccgt gcgcttccgc
     1441 ccggcgcccc tcccgaggtt tagcgttttt tcttatagct cttgatggcc gaggtcatgg
     1501 cccgcacgtt ctccacgtcg atcagcgcgg acatgccgca gccggcggag atgatgttga
     1561 accccgcgtc cgcggagcgc agggcgtccc gccggacctc ctccggggtg ccttgcagca
     1621 ggggcttgac cacgccgaca ttgcccacaa gggccgcttt cccgtcgact atctccaccg
     1681 ccttgtaggg gtcaaccttc tcctcgatgc tcagcccgtc cgccccggtc cgcatcatga
     1741 ggggcaggat gaccgtggtg ttgccgcata tgtggaggac cttcttggtg ttctccaggg
     1801 gctcgtacgt tcttttgata tatggcaggg cgtactcctc gaacatctcc ggggacatca
     1861 tgtcgtagga ggccgagggc tcgctcattt ggatgacgtc tatgcccagc gtctccacga
     1921 tcttcagcca ctccctctcg tactccgcgg cgaacttgac gaacttgtgc accttctcag
     1981 ggtcggtgac gatccagtag agcaggttct cggtccccac caggtggcca gcgatggtga
     2041 agggaccggt cacgccgagc agcgtgggca gctccttgcc cgctttctcc ttgatgatct
     2101 tggccgcctc gatgacgacc ttgttcctgg ccatctccat gatctccttg ggcttcggca
     2161 gcaccagctc ggcgtccggg tcgtcccggt acggatgcga cttcaccata ggcgtgctgt
     2221 tcttcttacc aaggaacacc ttgcatccca aggcctcagc ctcgtacgtg aggcagtagg
     2281 gcaccctgac cgattcgaag ccgaagaagt ccggggcgcc caacgcgatc ctgctcatca
     2341 gctcggcatc cgtgtgcgcc tctggccatg ccgcccctac cttgtccatg agctggaccg
     2401 tggcggtggt ggtcatcccg cataccggcg ggcgatcgac ctccttgcat gccatcgccc
     2461 ggaagaacct ctccctgggc ctcatctctc ccttgtcgtc cttcattctg ttcatcctcc
     2521 tttttccccg gcacggccgg ggagcgctga ccggaggacc tcgaccaaag gttgaagggg
     2581 tgagcgccgg cccgcgtatt tgcaatgagc ggcgaacctg ttcgctcccc ttccgttcaa
     2641 atagggaacg gcggacatgt tcgccttttg cgctccatcc accttgggcc gatgaagtgt
     2701 gcatcgctcg gaagagggcc ccgccgcaga aacgctgggc ttgtggaggc gaacatgtac
     2761 gccatcaacc ttaacctcct cgccgtgatg gtcaatcgct gaccgggacc ggggaccaag
     2821 gtgacggctc tctacaagag gggtgttaca ttggtagtca atgaggaact gaaaaagaag
     2881 atgctaaggt accggtgggt gatattcgga gtgttggccc tggcctattt cttcgtctat
     2941 ttccaccggg tgtccgtggc cgtggtctcg tcggacatca aggcacagtt cggtgtcggg
     3001 gcgagttcca tagccttgct gagctcggcg tacttctaca catacacgat aatgcaattg
     3061 ccgagcggat tactgaccga tagctggggc atcaagaaga cggcgggggt cttcaccttc
     3121 atcctggcca taggcgcggt cctgtgcggg gtggcgacca gctttgagat gatcctgctg
     3181 ggccgcctcc tgatagggct tggcgccgcc atggtgtaca tacctgtcat gaaggtgctc
     3241 gcgctctggt tcaggaagta cgagttcgcc tccgtcaacg ggatattgct tgcggtgggc
     3301 agcataggct ccctgagcgc cgccacgccc ctggccctga tgtccgacgc cctggggtgg
     3361 caggaggtgt tccttctgtt ggggatggta tccgtggtga ccgccatcct gatattcatt
     3421 ctggtcaagg accacccgaa ggacatgggc ttcccgacca tcccggagat cgaggcgttc
     3481 gagaagggcg aaccccttcc cgagacgtcc gcccaggagg ccaaggccga ggagaaggtc
     3541 cccatgggca aggccctcaa ggacgtgttc ggctccggcc tcaagttctg gccgctggcg
     3601 atatggttct tcttcatgta cgggagcata atggtgtacc aggggctttg gggctcccag
     3661 tacttccggg acatattggg ctgggacaag ggcacctatg ccctgacgat caccttcatc
     3721 ggcatcggca tgatcttcgg cagccccctg gccgggacca tctcggacaa gatccactcg
     3781 aggaagaagg tgctggtgat cgggaccgtc tgctacaccg cggtctgggc cgcattgctc
     3841 ctcctgatgg gggagtgcga gagcgaactc gtgtaccacg cgctgcactt cctgttcggc
     3901 ttcttcggcg gcttcttcgt ggtgtgctac gcgatgatca aggagcactt ccccacggcc
     3961 atcgccggaa cgtcgacggg cgccctgaac ctgttcccct tcgccggcgg ggccatcctg
     4021 cagctgctga gcgggtacat gatcctggac tattcgctga gcgagtaccg gagcgtctgg
     4081 ctgttcatgc tggtcggcat ggtcatcgcc tgcgcgtgca tcttcttggc caaggagagg
     4141 accaaggaag gggcctgaga ggaccagaaa aaggcgcgcc atcgcgcccc ctttctattt
     4201 ttaccctttt tttcttcaaa cagctctcct gagcctgccc atctcagcac ccactcgaca
     4261 gctgggcagg caggactgct gaagggatgc ctggggacca tatcgatgcc ttcccccggt
     4321 cttcttttta agggagggga gaagaggccg tgggccagca agaagatgta tgcactcgat
     4381 ccttcgaggg aaccggcaga gagatggccg cgtatcaggg gacggtcggt gaggccccgc
     4441 cggtcagacg cccaatcggt ccgtcagggc ggagaggagc gttttggcct ccgccctctc
     4501 gggccggacg tcgaccttga ccccgaacgc ctccagccat tcctccgtct ccctgcccat
     4561 ggccgctatc gtgatgccct cgagcatgcg gggtgcgtcg tcgccgcagc gctcctgcag
     4621 ctgcagcatg aaggcctggg cgtgagcggg ggtagggagg accagcgcgt ccacctcgcc
     4681 gtcctcgatg cggtccagga gggcttgcat gccctccccc tcatccagga ggagggtgta
     4741 caccggcacg tcctccacct cccagcccgc ggcctggagc cccctcacca tacgctcgga
     4801 gccctgatct gagcggagca gggcgaccgc tccgcggtgg ggggagcggt tgacgtgctc
     4861 aatcagagca tcggtcgtgg cctccggggc gatcgatgac acggtgaggc cgttgcgggc
     4921 ggcgccctgc gccgtgcgct ccccgatcac caccagctcg gtgcgcacca ccgcctccct
     4981 caagcggtcc agcatcctcc gcttccttgc catggccacc gcagcctcga tcgccattgg
     5041 gcaggtggcg atcagcatgg aatagcgtcc ggaaagcagg tccactagga acagggagaa
     5101 tacggagtcg tccctgggca cggcccgcag gggctgggcg aaggtgatat cgatgagctg
     5161 cctatggtcc ctcagaaggg gggcccattg ctccgggggg ccgaggatga ccacctgctt
     5221 cctcatcccg cggaggatgg gcggcgggca tatacaactt tgccttgcac cattcccgat
     5281 taccatctcc caacgaccac cgcgaacatc tggccccggg gggagggagg gtgcgacggg
     5341 tccccgagga cgatgcgctc ctccggatag cccatggcct cgcacaaggc tatccggcag
     5401 tcgatcccct tggccagaag cgcggccgcc gtggccctcg gcttcaaaca cttggaccca
     5461 ggggggagga tggcggaggc catcctgaac aacgtgagcg gagggaggca ccggaggaag
     5521 gccacggagg ccgtggaacg gctggccaag gtcccggtgc tcggggcggt cgagcgggga
     5581 agcccccttc cggaaaggca cctgaggctc gttcccgcgg aggagcaagg agatctaaag
     5641 gcgctgaggg cggggctcag gccttgattg aggaggtgga cctggagatg cttctcgaga
     5701 taggggagaa ggcggggccc atggagccca gggacgcccc cgtcctccca gaggggagcg
     5761 cggacatccg ggtggcggtc ccccgggacg cctccttctg cttctcttac ccagggaacc
     5821 tcgaggccct gcagcgcgcc ggcgggcgcc tcaccttctt ccgccccact gagggcgagc
     5881 cactgccgga ggccgacctt tattacctgg gaggggggta tcccgaggcc cacctggagg
     5941 agctcgcctc caaccgggac ttcctggaag gcctccggac cgccgcggag gacggc
//
LOCUS       DAOSYN010000074         5848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_11487_length_5848_cov_3.097359, whole genome shotgun sequence.
ACCESSION   DAOSYN010000074 DAOSYN010000000
VERSION     DAOSYN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11487_length_5848_cov_3.097359"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLJ11_04235"
     CDS             complement(<1..204)
                     /locus_tag="PLJ11_04235"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HOO03912.1"
                     /translation="MGRKVERTEEEWRRELDPEQYRALRRKGTEAPFTGRYYRTKDKG
                     TYLCAGCGRELFASEDKFDHGCGW"
     gene            complement(648..1145)
                     /locus_tag="PLJ11_04240"
     CDS             complement(648..1145)
                     /locus_tag="PLJ11_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOO03913.1"
                     /translation="MVSSVPYEVVRHLGDAELRRYGTWLLATVYDLDEDEAFQMLFQY
                     IDGDNALGTRFPIVVPVMARAGEPTDPESEGQMSFVLPYGLDLVDVARPNDPWIVLET
                     EEGGLFAVIRFRGMAEGADLLGRMNDLMTGLKEADYEMDVRPFDAVQFAVNACVRATA
                     RWPCA"
     gene            complement(1139..1366)
                     /locus_tag="PLJ11_04245"
     CDS             complement(1139..1366)
                     /locus_tag="PLJ11_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03914.1"
                     /translation="MSARVEMKGPLLAASAPAIGRKVFEPVRGEGAMWGGGRSGPLGG
                     RVPQAHCGFGPSSPGVADRRHGWPWRRGLAW"
     gene            complement(1412..2239)
                     /locus_tag="PLJ11_04250"
     CDS             complement(1412..2239)
                     /locus_tag="PLJ11_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO03915.1"
                     /translation="MLYVRAIKGGFGHRFALARLTRWPLLGRAVARALFDGDEIYVLP
                     KGSSVTLREIPLDIPVPDREDVVLPSEIVRYFIERSKHHFIMDRCICRTSAHCRDYPH
                     ELGCIFLGKAVLAIDPRLGRLVSKEEALAHLDRCEEAGLVHIIGRNKLDSIWTAGRPK
                     EDLMTVCNCCPCCCLWKMLPQLDEGISSKMKRLPGVHLTVDAARCTGCGTCVDGCYVR
                     AISMREGRAVIDQAMCKGCARCAHACPEKAITLHIEDGSYSMHAVERIAPLVDVTKD"
     gene            2356..2655
                     /locus_tag="PLJ11_04255"
     CDS             2356..2655
                     /locus_tag="PLJ11_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03916.1"
                     /translation="MLGTMEIEEVAKALSSDTRLKIIRLLAEGEASAVSVFKKYNERF
                     SDRRERATIYRELEVLYKSNFLVKTYREDSKEIVYRLAARSINIDLITQTVSAGP"
     gene            2790..2999
                     /locus_tag="PLJ11_04260"
     CDS             2790..2999
                     /locus_tag="PLJ11_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03917.1"
                     /translation="MVTVELTFGVPDKVADEVKKKFKGEKDILRVTKANPAFPPYYSV
                     GDPRAKPEKIKVDGKEYWVYRCFPG"
     gene            3213..3422
                     /locus_tag="PLJ11_04265"
     CDS             3213..3422
                     /locus_tag="PLJ11_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03918.1"
                     /translation="MVTVELTFGVPDKVADEVKKKFPDEKDILRVTKANPAFPPYYSV
                     GDPRAKPEKIMVDGKEYWVYRCFPG"
     gene            3517..3990
                     /locus_tag="PLJ11_04270"
     CDS             3517..3990
                     /locus_tag="PLJ11_04270"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO03919.1"
                     /translation="MVEVRLVPMGRDEFEAMLEASVRRYAEENVKVGYWNPDGALLRS
                     QQQHDLLLPDGLRSEGHHFYTAVDTETDVAVGHIWMRVEPGKERRGFIFDVYIEEGMR
                     GKGYGRAMMLALEGKARRMKLCSLALHVFAYNSVARHLYGSLGYEVKSLNMLKEL"
     gene            complement(3996..4274)
                     /locus_tag="PLJ11_04275"
     CDS             complement(3996..4274)
                     /locus_tag="PLJ11_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03920.1"
                     /translation="MAVSYGPFNRTTKERALPGKLPSNGAAPTIHPYAALIIHLLMES
                     GRYDHAAIMDDPRDLDRPSALRGEYLDRSIELRNEFVTMMGWQFKGLV"
     gene            4364..4738
                     /locus_tag="PLJ11_04280"
     CDS             4364..4738
                     /locus_tag="PLJ11_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOO03921.1"
                     /translation="MRCTHCSKCCQETRMELCRADIARLIRRGYADGTFYSRGEDGIP
                     RLINVNGHCVLFDPDGRRCKEYRSRPLGCALYPVNLTEDGVVVIDDLCPEAGSLGAEE
                     VREKGERLRRLIATIDAETSRH"
     gene            5029..>5848
                     /locus_tag="PLJ11_04285"
     CDS             5029..>5848
                     /locus_tag="PLJ11_04285"
                     /inference="COORDINATES: protein motif:HMM:NF018332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO03922.1"
                     /translation="MFTGKRARKAQVDGAAQDQQRNLSTRNRTFIMTGLALGMLLASL
                     DQTIVGTCLPKIVGELGGMSLYTWLFTAYMLANTVTIPIAGKMSDRYGRKPVFLLGMG
                     LFMAGSILAGMSNSMEQLIMFRGVQGLGAGCMMPVSMATVADLYAPTERGKIQGILGT
                     MFAVSSVIGPFIGGFIVDNLSWRWVFYVNIPVGVLAIAVTSLRFPNQSTDLDRPIDYL
                     GMVFLTLALTPALLVVSWGGTRYDWSSPLILGMSVLALASVVIFVHMERRAADPI"
BASE COUNT         1170 a         1839 c         1670 g         1169 t
ORIGIN      
        1 ccagccgcat ccatggtcga acttgtcctc ggaggcaaac agctccctgc cgcaccctgc
       61 acacaggtat gtccctttat ccttcgtacg ataatacctc ccggtgaagg gagcctcggt
      121 gcccttcctc ctgagagctc gatactgctc ggggtccagc tctcgcctcc attcctcctc
      181 ggtcctctcg accttgcgcc ccatcgccac cccgccgtta tatggtcggc catgtttatc
      241 tcgattgctc ccagacctgt gcggatgctt cggccgctta ttgccgcacc cccggcatca
      301 ttcccatacc ggccgcggcg ctcgctccgc tcccgccctt ttccattaag gccaacccgc
      361 gcccccgtca tgggaatgcc tggcgatccg aggatacagc cttcgaccat ctacgatatc
      421 cttttgccgt tcgctccatc caaggtgacc catatgggcg cggacccctc gatcgaacaa
      481 atggtggccc tcaggggcgc tcccgacaaa aaatagggaa cgccctcccc ttccagaact
      541 agtcatgggc cggtcgccaa cgcctccggc cccccgggaa cattgggcgc cgtgtcccta
      601 tcctcgttcg ttcgaacggc ctcattcggt cccaatcgat cccggggtca cgcgcacggc
      661 cacctcgctg tggctcgaac gcaggcgtta acggcgaatt gtaccgcatc gaaggggcgc
      721 acgtccattt catagtcggc ctccttgagc cctgtcatca gatcattcat cctccctagc
      781 aggtccgcac cctcggccat tccgcggaac cgtatgacag cgaaaagccc gccctcctcc
      841 gtctcaagca cgatccaggg atcgttcggc ctcgctacgt ccacgagatc aaggccatag
      901 ggcagcacga aggacatctg cccttccgac tcgggatcgg taggctcccc cgcgcgggcc
      961 atcaccggga ccacgatcgg gaacctcgtc ccgagggcgt tgtccccgtc tatatattgg
     1021 aagagcattt gaaaggcctc gtcctcatcc agatcataga ccgtcgccag cagccaggtg
     1081 ccatagcgcc gtagctcagc atcccccaga tgcctaacca cttcgtatgg cacgctgctc
     1141 accatgccaa ccccctccgc catggccagc catgccgacg gtcggcaacg cccggagagg
     1201 acggcccgaa accgcaatgg gcctggggaa cgcgccctcc aagggggccg ctcctacctc
     1261 cgccccacat cgccccttcc ccccgaacag gctcgaagac ctttctgccg atggccgggg
     1321 cagaggcggc gagcaagggt cctttcatct ccacccgagc gctcatgcta tcaccattct
     1381 cggaagggac tataaatcct catgcgggcc ctcagtcctt cgtcacgtcc accaaggggg
     1441 cgatccgttc caccgcatgc atcgaatagc ttccgtcctc gatgtggagg gtgatggcct
     1501 tctccgggca ggcatgggcg catcgggcgc agcccttgca catggcctgg tctataacgg
     1561 cccgcccttc cctcatggag atggcgcgca cgtagcatcc gtccacgcat gtcccgcaac
     1621 cggtgcacct ggccgcgtcc accgtcagat ggacgcccgg aagacgtttc atcttgctcg
     1681 agatgccctc atccaactgc ggaagcatct tccaaaggca gcagcaggga cagcagttgc
     1741 aaacggtcat caggtcctcc ttgggccttc ccgccgtcca tatactgtcc agcttgttgc
     1801 gccctatgat atgcaccaag cctgcttcct cgcatcggtc aagatgggcc agtgcctcct
     1861 ccttgctcac caatcgcccc agccggggat cgatggcaag caccgccttg cccaggaaga
     1921 tgcatcccag ctcgtgcggg tagtcccggc agtgcgccga tgtgcggcag atgcagcggt
     1981 ccataatgaa gtggtgcttg gagcgctcga tgaagtatcg aacgatctcc gacgggagga
     2041 caacgtcctc ccggtctggc actggaatgt ccaggggaat ctccctaagc gtgacggagg
     2101 accccttcgg aaggacgtag atctcgtccc cgtcgaagag cgccctggcc acggcccgcc
     2161 ccaggagcgg ccacctggtc agcctggcca atgcgaagcg gtggccaaac ccgcccttga
     2221 tggcgcgaac gtagagcatc gtcatccggc ccagacaatc accttcttgc aaggaacggg
     2281 atgtccccta atcaacgctt cgccgccctg ctcaagggtg cagggacgaa tcaaaactaa
     2341 ataggccgga aggcgatgct cgggactatg gagatcgagg aggtggccaa ggcgctttcg
     2401 tcggataccc gccttaagat aataaggcta ctggccgagg gtgaggcgtc cgctgtcagc
     2461 gtcttcaaga agtacaacga acggttcagc gaccgtcggg aaagggccac catctatagg
     2521 gagctggagg tcctgtacaa atcaaacttc ctcgtgaaga cctacaggga ggacagcaag
     2581 gagatcgtat accggcttgc ggccagatcg atcaacatcg acctcatcac ccaaacggtg
     2641 tccgccggcc catgatgccc caccccatcc caggacggtt acatgatatg gtgactggtt
     2701 acgcgccaac gtaacaagtg tttataatgt attaacggat tcactaaaat gttggtagcg
     2761 gtccgcgccg ctacccagga ggaaaacgaa tggtaacagt cgaactgacc ttcggtgtgc
     2821 ctgacaaggt cgccgatgag gtgaagaaga agttcaaggg cgagaaggac atcctgcgcg
     2881 tcaccaaggc caacccggcc ttcccgccgt actactccgt cggagacccg agggcgaagc
     2941 ccgagaagat caaggtcgat ggcaaggagt actgggtcta tcgctgcttc ccaggctgag
     3001 gcccccgtcc ttcgcccaaa cccattccat ttatctcatt tgccctttgt ccttttgtcc
     3061 tcgacggttc gtcccttcta cggtcgttgc gttccctcgg aacaggtatc atcggaatac
     3121 aacgggtttc accagcatgt aacaaagcct tataggctat tgggtgttaa tatggtctag
     3181 cggtgaaagc cgctgcaaag gaggaaaatc atatggtaac cgtcgaactg acgttcggtg
     3241 tgcccgacaa ggtcgccgac gaagtgaaga agaagttccc agacgagaag gacatcctgc
     3301 gtgtcaccaa ggccaacccg gccttcccgc cgtactactc cgtcggagac ccgagggcga
     3361 agcccgagaa gataatggtc gatggcaagg agtactgggt ctaccgctgc ttcccaggct
     3421 gatcggcctt cggccgaacc ttttttttct tttccatttc atagcgtcca tttgaaatag
     3481 catttctgcg atcgtcctcc gatctaggcg agctgtatgg tcgaggtgcg cttggtcccg
     3541 atgggcaggg atgagttcga ggccatgctg gaggccagcg tgaggaggta cgccgaggag
     3601 aacgtcaagg tcggatattg gaaccccgac ggcgcgttgc tgcggtcgca acagcagcac
     3661 gatctgctgc tcccggacgg acttcgttcc gaggggcacc acttctatac ggcggtggac
     3721 acagagaccg atgtggccgt cgggcacatc tggatgcgtg tggagcctgg caaggagcgg
     3781 cggggcttca tcttcgatgt ctatatcgaa gaggggatgc gcgggaaggg ctacggcagg
     3841 gcgatgatgc tggcccttga gggcaaagcc aggcgcatga agctgtgctc cctggccctg
     3901 catgtgttcg cctataacag cgtggctagg catctgtatg ggtccctggg ctatgaggtg
     3961 aagagcctca acatgctgaa ggagctttaa aggggtcaga cgaggccctt gaactgccag
     4021 cccatcatcg tcacgaactc gttcctcagc tctatggagc ggtccaaata ctcgcccctc
     4081 aacgcgctcg ggcggtcaag gtccctggga tcgtccatga tggctgcgtg atcgtacctc
     4141 ccgctttcca tcaggagatg gatgatgagg gccgcgtacg ggtggatggt aggggccgct
     4201 ccattcgatg gcagttttcc aggaagggcg cgctccttcg ttgtacggtt gaacgggccg
     4261 tagctgactg ccatgcgccc ccttccgtgc taggcacggg ctcgatctgt ggatgcgttg
     4321 tgcgccctca cgattatatc gccgctcggc catcttccat ccgatgcgct gcactcactg
     4381 ctccaaatgc tgtcaggaga cccggatgga gctctgcaga gcggacatag cccggctcat
     4441 cagaaggggc tatgccgacg gcaccttcta ttcgaggggt gaggacggca tcccccgcct
     4501 gatcaacgtg aacggtcact gcgtgctctt cgatcccgat ggacggaggt gcaaggagta
     4561 tcggtcgcgc cctcttggct gcgccctcta tccggtcaat ctgaccgagg acggcgtcgt
     4621 ggtcatcgac gacctctgcc cggaggctgg ctctctgggc gcggaagagg tcagggagaa
     4681 gggggagcgg ctgcgaagat tgatcgcgac catcgatgcc gagacatcca gacattgaat
     4741 ctacatagcc aataggaatc gttttgaaaa ctcagggcat agctggtcgt tccccacctc
     4801 tggtttgccc attctaaata gcatcccaga cagcatttga tgattatgat gcgactctgt
     4861 aaaataataa ataataaata gggggtgctc atcgacccta atggtcggac ggagcgttcg
     4921 acagcacatt gcacctctga tcgtcggtgc atcggcccat gggatggggt cggtgcctgt
     4981 cgataaaggg gaacacgcca agatatcgct catcgaggga atcatcacat gttcactgga
     5041 aagagagcgc gaaaggccca ggtcgatggt gccgcccagg accaacagcg caacttgagc
     5101 acccggaaca ggaccttcat catgaccggg ctggccttag gaatgctctt ggccagcctt
     5161 gaccagacta tcgtgggcac ctgtctgccc aagatcgtgg gcgagctcgg cggcatgagc
     5221 ctgtatacat ggcttttcac cgcctacatg ctggccaaca cggtcaccat ccccatcgcc
     5281 gggaagatgt ccgaccgcta cggacgcaag ccggtgttcc tgctgggcat ggggctgttc
     5341 atggccggtt ccatattggc cgggatgtcg aattccatgg agcaactgat catgttccgc
     5401 ggggtgcaag gactgggcgc agggtgtatg atgcccgtgt ccatggccac cgtcgccgat
     5461 ctctacgccc ccaccgaacg cggcaagatc caaggcatat tggggacgat gttcgcggtc
     5521 tccagcgtca tcggcccctt catcggcggc ttcatcgtcg acaacctttc ctggcgctgg
     5581 gtgttctacg tcaacatccc cgtcggggtc ctggccatcg ccgtcacctc cctgaggttc
     5641 cccaaccaga gcaccgacct cgacaggccc atcgactatc tgggaatggt cttcctgacc
     5701 ctggccctca cccctgcgct gctcgtggtc tcctgggggg gcacccgata cgactggagc
     5761 agcccgctca tcctggggat gagcgtcctt gccctggcgt ccgtggtgat cttcgtgcat
     5821 atggagcgca gggcggccga tcccatcc
//
LOCUS       DAOSYN010000075         5847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_11489_length_5847_cov_3.901070, whole genome shotgun sequence.
ACCESSION   DAOSYN010000075 DAOSYN010000000
VERSION     DAOSYN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11489_length_5847_cov_3.901070"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..720
                     /locus_tag="PLJ11_04290"
     CDS             82..720
                     /locus_tag="PLJ11_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HOO03923.1"
                     /translation="MDGKAILAGLEKAVISGNKNDAIKYSKDALAAGVKALDAIDNGL
                     VKGMTIVGDKYAKHEYFLPQVLLAANAMYGGLDILLPYIPKADAAKKVVGVIGVVEGD
                     VHDIGKNIVKTMLTAAGLDMHDFGKDVPIENFVNSVKNQKANLIAMSTLMTPTMDGMK
                     AVVDGLIENGDRMKVKIIIGGAPTSQAFADDIGADMHAINAQEAVKKVKEVL"
     gene            722..1819
                     /locus_tag="PLJ11_04295"
     CDS             722..1819
                     /locus_tag="PLJ11_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO03924.1"
                     /translation="MAEKMTHIQRCLASLADEPVDRLTVYPIACGVSRRTIGDGTMTY
                     KEWCSSPKNYAQGLISGQKYFDLDFTVGLMDLSVIAGDFGSHVRMDEQNTPFVDDPVI
                     KTVEDYETLQMPDPKKGRSGVILEGTKIFAEALKNETITSGFLEGPLLSLTQSASAEK
                     VFMDMYNNRSAVHKALGTFTDYLKEMVKGIGQAKPAACMWDYLWGSYSCLGDKEYDEF
                     EGQHKYAGSLNEAVGKEGMALAIHNCADLPHLDAQVKQMKPAVYTMAWYPLIPGSPSA
                     SDAIGKGYADNCVLCGTIDPQGFVRWSQEKMQKVTMNLIQEVKTALCARGLKSRYTIA
                     SGCEVPPTVTTRLENIKEVSDTVKKYGQVTC"
     gene            2284..2958
                     /locus_tag="PLJ11_04300"
     CDS             2284..2958
                     /locus_tag="PLJ11_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO03925.1"
                     /translation="MKIAQVAGFLGSGKTTFLIELVKILVDRGHRIAIIVNDVGEINV
                     DVRIVEMYGLKAKEISGGCICCQIAGNFANTIAILQKTFGPDIIVVEPSGVAIPWGLK
                     RAAEYSEEEMEGETIVHAPVVTLVDSLRIDELMNAVRRLVETQIREADVIFINKVDSA
                     SKEKVDLSVQFAKSINPRAEIMFGSGKNNQGVKEVADLIETRTSPRYDESYEKEILKK
                     DYGGKG"
     gene            2958..3431
                     /locus_tag="PLJ11_04305"
     CDS             2958..3431
                     /locus_tag="PLJ11_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03926.1"
                     /translation="MTELDEDDDLHLEMHRAVDELGKFSLHMEVEFGKGSKAERAISF
                     VEELLIKVTDACLHHGADLVGHVKAFMMVEDGSTVGFSLIHQNMKVNITRQLKGDVIE
                     RAEVIVHVIVHGLWDPEVRDQTMTVLEGLAKERGVPFRIIKDFFETEKSAAHHGG"
     gene            3434..3745
                     /locus_tag="PLJ11_04310"
     CDS             3434..3745
                     /locus_tag="PLJ11_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03927.1"
                     /translation="MDGYYQAMRSRGFSRDTVDSIRRFKCPNCGFEFSITYARTFACR
                     GCPEVMNNCPKVRCSKCDYEFYFRETPHITGELREREVSHHISAIVEDYYKQQGWKKS
                     R"
     gene            complement(3746..4123)
                     /locus_tag="PLJ11_04315"
     CDS             complement(3746..4123)
                     /locus_tag="PLJ11_04315"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOO03928.1"
                     /translation="MRKGVVVCGANGERGYFLERVIEAFSKDNMSKVIQFSVCTVGLS
                     PIMLEMGGVDMGSLVTVNGCRNRCCDRLMEENGLKVGASVTVDDRIERRLAPCEPTSK
                     FNFQEPTEAEVRTMVRAMEDAIR"
     gene            complement(4120..4473)
                     /locus_tag="PLJ11_04320"
     CDS             complement(4120..4473)
                     /locus_tag="PLJ11_04320"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOO03929.1"
                     /translation="MGQTSTIDVVICSGFSPGARVLRAAVRELADEGDIRVVTVAPAL
                     AGIPKAVAEMRALQDHKVVCVDGCEGGCGLQVLNRFEVLPSSIVMIEKHFNVTRKGID
                     EAKDRIRQAVREVQG"
     gene            complement(4451..>5847)
                     /locus_tag="PLJ11_04325"
     CDS             complement(4451..>5847)
                     /locus_tag="PLJ11_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOO03930.1"
                     /translation="LMIVKGDGSLMGEQLAKERPVETILSGPAASLIGARNLTGENDA
                     VVVDVGGTTTDIGVLRGGRPRMDPEGAIIGGWRTRVRAADISTSGIGGDSRVVVVNGQ
                     IMLGTLRVMPLCIASSQYPRVLERLRKLKDVKQTPQATHIALESILQADEFFIFSRMV
                     KGYEPSENERALIDLIRTEPKTLYEISEATGVHPYSYNVRKLEELGVITRIGFTPTDA
                     LHASGEYVEYDREAPLLSAEYRAGLCGMDVQRFCAEVKEAVIQKIAREIVYRLIYEDC
                     GKTVHCDVCSSMIDKMVSQLPGIDFSVDMRLHRKIIGIGAPIGAYLPSVAQRLHTELL
                     IPENSDVGNAVGAITGSIMESVEVLIKPKPGLGVMEDPPCTMHSMDEMREFETMSEAA
                     AYAKSWGENKVRRNAVTAGADEIEVLVENHRMMGQIGKSWGDGLTLEVHVKVTAVGKP
                     RMFFEVEHGSDEYD"
BASE COUNT         1192 a         1755 c         1770 g         1130 t
ORIGIN      
        1 ccataacaat ggtttataat ccctaatgac cgtgtgtttt ttacaccaat ggaattggtt
       61 ctgaggagag gtggttaaaa aatggacggg aaagcgattt tggctggttt ggagaaagct
      121 gtcatctcgg gcaataagaa cgatgccatc aagtattcca aggatgcgct ggccgctggc
      181 gtcaaggcgt tggacgccat agacaacggc ctggtcaagg ggatgaccat cgtgggggac
      241 aagtacgcca agcatgaata tttcctaccc caggtcctac tggcggcgaa cgccatgtat
      301 ggcgggttgg atatactgct cccctacatt cccaaggcgg acgccgccaa gaaggtggtg
      361 ggcgtgatcg gcgtggtcga gggggatgtc catgacatag gcaagaacat cgtcaagacc
      421 atgctgaccg ccgccggcct ggacatgcat gacttcggga aggatgttcc gatcgagaac
      481 tttgtgaact cggtcaagaa ccagaaggcc aacctcatag ccatgagcac cctcatgacg
      541 cccaccatgg atggcatgaa ggcggtcgtc gatggcctta tcgagaacgg cgaccgcatg
      601 aaggtgaaga tcatcatcgg cggggcgccc acgtcccagg cctttgcgga cgacatcggg
      661 gcggacatgc atgccatcaa cgcccaggag gctgtgaaga aggtcaagga ggtgttatga
      721 tatggcagag aagatgaccc atatccaaag gtgcctggcc tcgctggccg atgagccggt
      781 ggacaggctg accgtttacc ccatcgcctg cggggtgtcc cgccggacga taggcgatgg
      841 caccatgacg tacaaagaat ggtgctcgag ccccaagaac tacgcgcagg ggctgatctc
      901 cggacagaag tatttcgacc tggatttcac cgtcggcctc atggacctgt cggtcatagc
      961 gggggacttc gggtcccatg tgcggatgga cgagcagaac acgccgttcg tcgacgaccc
     1021 ggtgatcaag acggtggagg actacgagac gctgcagatg cctgacccga agaagggaag
     1081 gtccggtgtg atcctcgagg gcaccaagat tttcgccgag gcccttaaga acgagaccat
     1141 cacctcgggg ttccttgagg ggccgctgct ctccctcacg cagtcggcca gcgcggagaa
     1201 ggtcttcatg gacatgtaca acaacaggtc cgccgtccac aaggctctgg ggaccttcac
     1261 ggattacctg aaggagatgg tcaaggggat agggcaggcc aagcccgccg catgcatgtg
     1321 ggactatctc tggggttcgt attcctgtct aggggacaag gagtatgacg agttcgaggg
     1381 gcagcataag tacgctggtt ccctgaacga ggcggttggc aaggagggaa tggcattggc
     1441 catccacaac tgcgccgacc tgccccacct tgacgcccag gtcaagcaga tgaagcccgc
     1501 ggtgtacacc atggcatggt accccctgat ccccggctcg ccctcagcgt ccgacgccat
     1561 cggcaagggg tatgcggaca actgcgtcct gtgcgggacg atcgacccgc agggcttcgt
     1621 gcgctggagc caggagaaga tgcagaaggt caccatgaac ctgatccagg aggtcaagac
     1681 ggccctgtgc gcccgggggc tgaagtcgag gtacaccatc gcttccggct gcgaggtgcc
     1741 tccgacggtc accacccgcc ttgagaacat caaggaagtg tcggacacgg tcaagaagta
     1801 cggccaggta acctgctgaa ggaccgggcg tttccaaacc ccttccatct ttttcttttc
     1861 cctttgtctt gcctcaccga gcccgaccga acgtcttcag atcagccatg gtcctccatg
     1921 ttgggatcgg gacgctccga tgggacggac gataaggacc ttgtccagac cacggatgtg
     1981 cttcacatgt tcgaagggga tgcggaacct gccgagagga gggggcggat ttttttatgg
     2041 catagcgacg cgaacacgac cgggagggat agcgcctgag ggaaactgcc gcctggtccg
     2101 cggagcgctt cttagaagct ttgggcgggt cgatggaacg gtcctcggca gatggtcctg
     2161 ggacaacggc ggggatcccg ggcgggaagc ctccatccag gtagacgctc ggccgtctcc
     2221 gtgccacgga atcaacaacc tattcatact tggagcccga tttccgattt cactggtgta
     2281 tcgatgaaga tcgcacaggt cgcggggttc ttgggaagcg gcaagaccac tttcctcatc
     2341 gaactggtca agatcctggt ggaccgcggt cataggatcg cgataatagt caacgacgtg
     2401 ggcgagatca acgtcgatgt gaggatagtc gagatgtatg ggctcaaggc caaggagatc
     2461 tcgggcggat gcatatgctg ccagatcgcg ggcaactttg ccaacaccat cgccattctc
     2521 cagaagacgt tcgggcctga tatcatcgtg gtcgagccat ctggagtggc catcccatgg
     2581 ggcctcaagc gtgccgcgga gtactccgag gaggagatgg agggcgagac gatcgtccac
     2641 gcccccgtcg tcacgctggt ggactccctt aggatcgatg agctgatgaa cgcagtgcgg
     2701 cgcctcgtcg agacccagat aagggaggcc gacgtgatct tcatcaacaa ggtggacagc
     2761 gcctccaagg agaaggtcga cctctccgtt caattcgcca agtcgatcaa tccccgcgca
     2821 gagataatgt tcggctcagg gaagaacaac cagggagtga aggaggtggc cgatctcata
     2881 gagaccagga cctccccccg ctacgacgaa tcgtatgaga aggagatatt gaagaaggac
     2941 tacggaggca agggctgatg accgagctcg acgaagacga tgacctgcat ctcgagatgc
     3001 accgggcggt ggacgagctt ggcaagttct ccttgcacat ggaggtcgag tttggaaagg
     3061 gctccaaggc cgaaagggcg atctccttcg tggaggagct tcttatcaag gtgacggacg
     3121 cctgccttca tcacggcgcg gaccttgtgg gccatgtcaa ggccttcatg atggtcgagg
     3181 acggctcgac ggtcgggttc tcgctcatcc atcagaacat gaaggtcaac atcacaaggc
     3241 agctgaaggg ggacgtcatc gagcgggcgg aggtcatagt ccatgtcatc gtccacgggc
     3301 tttgggaccc cgaggtccgg gatcagacca tgacggtcct ggaaggcctg gccaaggagc
     3361 ggggagtacc gttccgcatc atcaaggact tctttgagac cgagaagagc gcagcccatc
     3421 atggaggctg agcatggatg gatactacca ggcgatgcgg agcagggggt tctcccggga
     3481 caccgtggac tccatccgca ggttcaaatg cccgaactgc ggcttcgagt tctcgatcac
     3541 ctatgcaagg accttcgcct gccggggctg ccctgaggtc atgaacaact gccctaaggt
     3601 gagatgcagc aagtgcgact acgagttcta tttcagggag acgccgcaca tcaccgggga
     3661 gctccgggag agggaggtgt cccatcacat ctccgccata gtggaagact attacaagca
     3721 gcagggctgg aagaagagcc gctgatcagc ggatcgcgtc ctccatggcc ctgaccatcg
     3781 tcctgacctc cgcctccgtc ggttcctgga agttgaactt cgaggtgggc tcgcagggtg
     3841 ccagccttct ttcgatccga tcgtcgacgg tgacggaggc cccgaccttg agcccgttct
     3901 cctccatcaa ccggtcgcag caccgattcc tgcacccgtt gaccgtgacc agggagccca
     3961 tgtccacccc gcccatctcg agcattatcg ggctgaggcc gacggtacaa acggagaact
     4021 ggatcacctt ggacatgttg tccttggaga aggcctcgat gaccctctct aggaagtagc
     4081 cgcgttcgcc gttggccccg cacaccacca cgcccttcct catccctgca cctccctgac
     4141 cgcctggcgt attcggtcct tggcctcgtc gatccccttc cgggtgacgt tgaagtgctt
     4201 ctcgatcatg acgatggagg agggcagcac ctcgaagcgg ttgaggacct gcagcccgca
     4261 cccgccctcg cacccatcca cgcagacgac cttgtggtcc tggagcgcac gcatctcagc
     4321 cacggccttg gggatccccg ccagggcggg ggccacggtc acgacccgga tgtccccctc
     4381 atcggccagc tcgcgcacgg cggcccgtag caccctggcc ccggggctga acccgctgca
     4441 tatgaccaca tcaatcgtac tcgtctgacc catgctctac ctcgaagaac atgcggggct
     4501 tgcccaccgc ggtcaccttc acatgcacct ccagggtcag cccatccccc cacgacttcc
     4561 ctatctggcc catcatcctg tggttctcca ccaggacttc gatctcgtcc gctccggccg
     4621 tcactgcgtt cctgcgcacc ttgttctccc cccaggattt ggcataggca gcggcctctg
     4681 acatggtctc gaactccctc atctcgtcca tggagtgcat ggtgcagggg gggtcctcca
     4741 tcacccccag gccgggcttg ggtttgatga gcacctcgac gctctccatg atgctgcccg
     4801 tgatggcccc caccgcgttc ccgacgtccg agttctctgg tatgagcagt tcggtatgca
     4861 gccgctgggc cacggaaggc aggtacgccc cgatgggggc cccgatgccg atgatcttcc
     4921 ggtgcagtcg catatcgact gagaaatcga tgccgggaag ctgactgacc atcttgtcga
     4981 tcatcgagga gcatacatcg caatggacgg tcttgccgca gtcctcgtag atgagccggt
     5041 agacgatctc ccgggcgatc ttctggatga ccgcctcctt gacctcggcg cagaacctct
     5101 ggacatccat gccgcacagg cctgccctat attcggccga caggaggggt gcctccctgt
     5161 catattccac gtactccccg gaggcgtgca gggcgtcggt cggcgtgaac cctatccggg
     5221 tgatgacccc cagctcctcc agctttcgca cgttatagct gtagggatgg acccccgtcg
     5281 cctccgaaat ctcgtacagg gtcttcggct ccgtcctgat gaggtcgatg agggctcgtt
     5341 cgttctcgga cggttcgtag cccttcacca tccttgagaa gatgaagaac tcatcggcct
     5401 gcaggatgct ctccagagct atgtgggtgg cctggggggt ctgttttaca tccttgagct
     5461 tcctgaggcg ctccaacacc cgggggtatt gggaggacgc aatgcagagg ggcatcaccc
     5521 tcagcgtgcc gagcattatc tggccgttga ccacgaccac ccggctatcg ccccctatgc
     5581 cggaggtgga gatgtccgcc gctcggaccc ttgtccgcca gccacctatg atggccccct
     5641 ccgggtccat ccttggcctc ccgcccctga gcacgccaat gtcggtggtg gtgccgccga
     5701 cgtccacgac caccgcgtca ttttcccccg ttaggttgcg ggccccgatc aggctcgcgg
     5761 ccggcccgga gaggatggtt tccacaggtc tttcctttgc cagctgctct cccatcaatg
     5821 acccgtcccc cttcacgatc atcaacg
//
LOCUS       DAOSYN010000076         5713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_11832_length_5713_cov_3.899965, whole genome shotgun sequence.
ACCESSION   DAOSYN010000076 DAOSYN010000000
VERSION     DAOSYN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11832_length_5713_cov_3.899965"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PLJ11_04330"
     CDS             complement(<1..970)
                     /locus_tag="PLJ11_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03931.1"
                     /translation="MRSQRILAALLVLALLTIVPSAPVQGDGAFDSPISVTDSRGEVT
                     NLTGPAVHVASFGAFATNTLVDIGMIDKAVIFDAGSEFDKSGIPEMRNISEDRFVMVS
                     SANKDMVVQRMLALVDEDRWNKSSDVVLGHGYSYLSSVWNALEDRGFHVVTFYPDSYD
                     GIVQVVEDIETITGADHDVSGHMVFVKEHIAERLEEEGITNDSEKIRAVYVSYSNNLM
                     WLANTGSVTVDFIAFAGGVNVAEDPTKTSPRYSADLSAILLLYPRPDIVLLDGLYPWT
                     AEEFKSKIGNDDIIVYKLNKSWNSYCPDAAEGLWAVAHLFYPEYFEG"
     gene            1193..1522
                     /locus_tag="PLJ11_04335"
     CDS             1193..1522
                     /locus_tag="PLJ11_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03932.1"
                     /translation="MASRVKVVTPICSHETWVTAEMDGDDRLRVRIESDCANVRNYAE
                     RLRSVTMDDINEQRGSRILTEAQDGILTPTCLVPTAVFNACWVEAGMISKRLAVKEGS
                     VAIQFVD"
     gene            1587..2666
                     /locus_tag="PLJ11_04340"
     CDS             1587..2666
                     /locus_tag="PLJ11_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HOO03933.1"
                     /translation="MCGTTAFFYNDGLLRYQFGPEHPYQPIRFQLIRDTLRDMDVFND
                     RLVYVEPPAATEEDLREVHDPALIAYVKELSAIGSGYLDLGDTPVCQGLYEGALAVVG
                     ATLHGAESIARGEYDHVMTPGGGLHHARRDKAAGFSVFNDLAIAARRLQRRYGYERIA
                     IIDIDGHHGDGTQSIFNGERVLTISFHRYGAGFYPGTGAVTDIGEGEGEGYAINVPLP
                     RCTPDDVYVPTYERVVTEALDAYRPEIIIHQFGTDAHYSDQLVGMGLTTRAYEKVAEI
                     THRLAHEHCDGRYLVTGGGGYSIDAARRIWSIAVCTVSGSFPLNPEGLHLLRDSGLNR
                     KWKVQQEEFHKRLDYLFEEVIPKIC"
     gene            complement(2647..3282)
                     /locus_tag="PLJ11_04345"
     CDS             complement(2647..3282)
                     /locus_tag="PLJ11_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03934.1"
                     /translation="MARQQGLLVSTFGANVSEIVPGLRSLPHDHLILVVETTADESAA
                     TAFLDRLGVRYEALTVDPQDLAAAAMRIVQRVQGQCWKGWSVCFDITGGRKLLSDTAM
                     LAALAIGAEVCCYETKACRFPLLAARGVEEALPREIADALLRTKWPFPLATVQELDGR
                     HPLAILMRRMREMDLIEPTVAGKAPALALTGRGRACLDWLQRMEGISRSWE"
     gene            complement(3282..3926)
                     /locus_tag="PLJ11_04350"
     CDS             complement(3282..3926)
                     /locus_tag="PLJ11_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOO03935.1"
                     /translation="MVAILGTLGFRARSLLPTVESTPSLERAVVFCNDHPRALEALAE
                     VDSVCTVMGIELRRVAIPDAFDLLQAARAMRTEVRALRRKGEEIAVFNIAGGTKMMSA
                     AALLICAMEGLNSVYVHDETYQEIPLPLLRVDYSNLLTPAQKEVLAFIWQNRSHPMTQ
                     MEIAKGIGKHKATVNHHIQVLEGKGILHLTEDAADRRRKVVTVDDALELMMELD"
     gene            4085..>5713
                     /locus_tag="PLJ11_04355"
     CDS             4085..>5713
                     /locus_tag="PLJ11_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO03936.1"
                     /translation="MVVTAFLLTLALLPLVHVPSFEATAPTFMTSSMSYEYHFSEPFV
                     DLTSADRLFRIEGSDLDPRGGVPALPVFPVSLAVPPGQRLVDLVMSHSAPRMIELMTA
                     YPTNPVEMAVDGEMAYCHEYVAEPWEATGSQILEGVEVVGLTLRPLYWNAGTGALSFV
                     EDFTLTLILEDARPDYVGDMGRVREMVDNPEAVPDLLPFASSNVLPQGEYDHLIITSE
                     ALSSPFEELARWKGERDERGAVYEGVRSKVVTLEDILATKALWGSPSSHHGTGNDTQT
                     VVRNMIVAAHREWGVDYVLIGGDDDVVPVRMVAAPIFDTEYRELPADIYYSGLDGDWD
                     ADGDGVYGELLSTFPTVRDEADLLAEVFVGRATVSTAEGAWNFVNKTMAYERGYVNQY
                     ADDILLIGERLDSAPTYGDDYKQEIFDEVLADEGLTSSTLYARDGTFSGPAVLAAMRS
                     GVHVINHMGHGNFGSFAGVTSQDVQGLDNALPFLLYTQACMVAGFDERADDPGDCIAE
                     EMVQGEGGGGLHRQLPLWVVCQGQHVRPIAAVRQG"
BASE COUNT         1078 a         1704 c         1794 g         1137 t
ORIGIN      
        1 cgccctcgaa gtattcggga tagaacaggt gggccaccgc ccacaggccc tcggcggcgt
       61 ccgggcaata ggagttccag gacttgttca gtttgtagac gatgatgtcg tcgttgccga
      121 tcttgctctt gaactcctcc gctgtccatg ggtagagacc gtcgagcagg acgatgtccg
      181 ggcgcgggta cagcagcagg atggctgaga ggtccgcaga gtaccgcggg gaagttttgg
      241 tggggtcctc ggcgacgttg acgcctccag cgaatgctat gaagtccacg gtcaccgagc
      301 cagtgttggc cagccacatg aggttgttgc tatagctgac gtacaccgcc cggatcttct
      361 cggaatcgtt ggtgatcccc tcctcctcga gcctctccgc gatgtgctcc ttcacgaaga
      421 ccatgtgccc tgagacatcg tggtcggccc ccgtgatcgt ttcgatgtcc tctaccacct
      481 ggactatgcc atcatatgaa tcgggataga aggtgaccac gtggaaaccg cggtcctcca
      541 gggcgttcca gacagatgag aggtaggaat agccatgccc gaggacgacg tcgctgctct
      601 tgttccatcg atcttcatcg accagggcca gcatcctctg caccaccatg tccttgttgg
      661 cggagctgac catgacgaac ctatcttcgc tgatgttcct catctcgggg atcccgctct
      721 tgtcgaactc agagccagca tcgaagatca ccgccttgtc tatcatcccg atgtccacaa
      781 gggtgttggt ggcgaacgcg ccgaacgagg cgacatggac cgccggtccg gtcaggttgg
      841 tgacttcccc cctggaatcg gtcacggaga tggggctgtc gaatgcgccg tccccttgga
      901 cgggggcgga gggaacgatg gtcagcaagg ccaggaccag tagcgcggcc aggattctct
      961 gggacctcat ggtgcattcc tcttgtgagc ggttttgata atattattac agtataaagt
     1021 attatcttat tcgtaatatc gttcttcgtt ccatctgaac gggcggccca cttccttgta
     1081 aggagcgcat gagcggagac cgcatagaga ttccgaactt ggtttatcaa ggctgatcga
     1141 tgcagaatcg gtgtgaaagg gagttatacc aaatcgtacc ttcgcaataa tcatggcgtc
     1201 gcgggtgaag gtggtcacgc ccatatgctc gcacgagacc tgggtgaccg cggagatgga
     1261 tggggatgac aggctcaggg tgcgcatcga gtccgattgc gccaatgtga ggaactacgc
     1321 cgagcggctg cggagcgtca cgatggacga catcaacgag cagcgcggtt cccgcatcct
     1381 gaccgaggcc caggatggta tactgacccc cacctgtctc gtccccaccg cggtcttcaa
     1441 cgcgtgttgg gtggaggcag ggatgatctc caagcggctg gcggtgaagg aggggtcggt
     1501 ggctatccaa tttgtcgatt gagccggcaa aaaatatatt gcttcaatcg tcccttaaaa
     1561 aaaaggccaa cggataggtc ttcctgatgt gtggtaccac tgccttcttc tataacgatg
     1621 gtctgcttcg gtatcagttc ggtccggagc atccctacca gcccattcgg ttccagttga
     1681 taagggatac cttgcgggac atggacgtgt tcaacgaccg cctcgtgtac gtggagcccc
     1741 cagcggcaac ggaggaggac ctgagggagg tccatgaccc cgcgctcatc gcgtacgtga
     1801 aggagctgag cgccatagga agcgggtacc ttgacctggg ggacacccca gtgtgccaag
     1861 ggctgtacga gggggccttg gcggtggtcg gcgccaccct gcacggcgca gagagcatcg
     1921 cccgggggga gtatgaccat gtcatgaccc cgggcggtgg gctacatcat gcccgcaggg
     1981 acaaggcagc tggcttcagc gtgttcaatg accttgccat cgccgcgagg cgtctgcagc
     2041 ggaggtatgg gtatgagagg atcgccatca ttgacatcga cggccatcac ggggatggta
     2101 cccagtcgat cttcaacggg gagcgggtcc tgaccatctc cttccatcgc tatggcgccg
     2161 ggttctaccc gggcacggga gcggtcaccg acattgggga gggggagggg gagggatacg
     2221 ccataaacgt cccgctcccc cgctgcactc ccgacgacgt ctacgttccc acgtacgaga
     2281 gggtggtcac ggaggccttg gacgcctatc gccctgagat catcatccac cagttcggca
     2341 cggacgcgca ctacagcgat cagcttgtgg ggatggggct caccaccagg gcgtacgaga
     2401 aggtggcgga gataacccat cgcctggcgc atgagcattg cgacggccgg tacctggtga
     2461 ccggcggagg tgggtattcg atcgacgccg cccggcggat atggagcatc gccgtgtgca
     2521 ccgtctctgg ctccttcccc ttgaacccgg agggcctgca cctgctacgc gactccgggc
     2581 tgaaccgtaa atggaaggtg cagcaggagg agttccacaa acgccttgac tatctattcg
     2641 aagaggtcat tcccaagatc tgctgatgcc ctccatcctc tgaagccaat ccaggcaggc
     2701 cctccccctg cccgtcaagg ccagtgccgg ggcttttcca gccaccgtgg gctctatgag
     2761 gtccatctcc ctcatcctgc gcatcagaat ggcgaggggg tgacgaccgt cgagttcctg
     2821 caccgtcgcc agggggaaag gccatttggt tcggagcagg gcatcggcga tctctctggg
     2881 aagggcttcc tcgacccccc tggccgccag caacgggaat ctacacgcct tcgtttcgta
     2941 gcagcacacc tcggccccga tcgccagggc cgccagcatg gccgtgtcgc tcaacagctt
     3001 cctccctccg gtgatatcga agcacacgct ccatcccttc cagcactggc cctgcacccg
     3061 ctgcacgatg cgcattgcgg ccgctgccag gtcctgaggg tccaccgtaa gcgcctcata
     3121 cctcaccccc agcctatcca ggaaagccgt ggctgcgctc tcgtccgctg ttgtttccac
     3181 caccaggatg agatggtcgt ggggaaggga ccgcaggccg ggaacgatct cgctgacgtt
     3241 cgcgccgaag gtgctgacca gcaggccctg ttgcctggcc atcaatccag ctccatcatc
     3301 agctcgaggg cgtcatcgac cgtgacgacc ttcctgcggc ggtcggcggc gtcctccgtg
     3361 agatgcagga tgcccttccc ctcgaggacc tggatgtggt gattgacggt cgccttgtgc
     3421 ttccctatgc ccttagcgat ctccatctga gtcatcgggt gcgagcggtt ctgccatatg
     3481 aaggcgagga cctccttttg agcgggggtg agcaggttgg agtaatccac cctcaggagg
     3541 ggcaacggga tctcctggta agtctcatcg tgcacataga cggagttgag cccctccatg
     3601 gcgcagatca agagggcggc cgcgctcatc atcttcgtcc ctccagcgat attgaatacc
     3661 gcaatctcct cgcccttcct tctgagcgcg cggacctccg tgcgcatcgc cctcgcggcc
     3721 tgaaggaggt cgaaggcgtc cggtatggcg acccgcctca gctctatgcc catgacggtg
     3781 cacaccgagt cgacctcggc cagggcctcc aatgcgcgcg ggtggtcgtt gcagaagacc
     3841 accgccctct ccagagaggg cgtcgactct acggtaggaa gcagggacct ggccctgaag
     3901 cccaatgtgc ctaggatggc gaccatgata tcatctcatc atcagtggaa tgctttaatt
     3961 caatttccta agacggtatt attattaatg atactaattt gaaggtcctg tggtaaatcg
     4021 aaataggtac ggacccctta cctcttcatc ggatagaacg atgcgccccc ctcatcgcgc
     4081 ccgcatggtc gtaacggcct ttctactgac cctggccctt ctccccctcg tccacgttcc
     4141 ttccttcgag gccaccgcgc ccaccttcat gacctcctcc atgagctatg agtatcattt
     4201 ttcagagcct ttcgtcgacc tgacgagcgc ggaccgcctc tttcggatcg agggctcgga
     4261 ccttgacccc cggggagggg tcccggccct gccggtattc cccgtgtccc tggcggtccc
     4321 ccccgggcaa aggctcgtgg acctggtcat gagccattcc gccccccgga tgatcgagct
     4381 gatgaccgcc tatccgacca atccggtcga gatggcggtg gatggggaaa tggcctattg
     4441 ccatgaatat gtcgcggagc cgtgggaggc gacgggaagc cagatccttg agggggtgga
     4501 ggtcgtaggg cttaccctcc ggccgttgta ttggaacgcg ggcacggggg cgctgtcgtt
     4561 cgttgaggat ttcacgctca ccctgatcct ggaggatgcc cggcccgatt atgtcgggga
     4621 catggggagg gtgagggaga tggtcgacaa tccggaggcg gtgccggacc tactgccctt
     4681 cgcaagctcg aacgtcctcc cgcaggggga gtacgaccat ctcatcatca ccagcgaggc
     4741 cctttcctcc ccattcgagg aattggcgag gtggaagggg gagagggatg aacggggggc
     4801 cgtgtacgag ggcgttcgga gcaaggtcgt gaccttggag gacatcctcg ccaccaaggc
     4861 cctttggggc tcgccatcct cgcatcacgg caccgggaac gacacgcaga cggtggtccg
     4921 caacatgatc gtcgccgccc acagagagtg gggggtggac tacgtcctca ttggagggga
     4981 cgatgacgtc gtccccgtcc gaatggtcgc cgcgccgata ttcgacacgg aataccggga
     5041 gctgccggcc gacatctact actccggtct ggatggggac tgggacgcgg acggggacgg
     5101 ggtgtacggg gagctgctca gcaccttccc gacggtgcgg gacgaggcgg acctgcttgc
     5161 cgaggtgttc gtgggcaggg ccacggtatc gaccgccgag ggcgcctgga acttcgtgaa
     5221 caagacgatg gcgtacgaga gggggtacgt gaaccaatac gcggacgaca ttctgctgat
     5281 aggggagcgg ctcgactcgg cccctaccta cggcgatgat tacaagcagg agatcttcga
     5341 cgaggtgctt gccgatgagg ggctgacatc ctcgaccctt tacgcgaggg acggaacgtt
     5401 ctccggcccc gccgtgctgg cggcgatgcg gtcgggcgtg catgtgatca accacatggg
     5461 ccatgggaac ttcgggtcct tcgccggcgt aaccagtcag gacgtccagg gattggacaa
     5521 tgcccttccc ttcctgctct acacccaggc ctgcatggtc gctggcttcg acgagagggc
     5581 ggatgacccg ggggactgca ttgcggagga gatggtgcag ggcgaggggg gcggtggcct
     5641 tcatcggcaa cttccgctat gggtggtatg tcaggggcag catgtacggc ccatcgcagc
     5701 ggttcgacaa ggc
//
LOCUS       DAOSYN010000077         5651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_12008_length_5651_cov_4.561830, whole genome shotgun sequence.
ACCESSION   DAOSYN010000077 DAOSYN010000000
VERSION     DAOSYN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12008_length_5651_cov_4.561830"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..575)
                     /locus_tag="PLJ11_04360"
     CDS             complement(204..575)
                     /locus_tag="PLJ11_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOO03937.1"
                     /translation="MREVRADGAPAPVGPYSQAVVCGDLVFCSGQLGLDPATGAMAEG
                     AVAQARQALANLQAVLQAAGSSLDRAIKVTVYMTDLAHFPEVNKAYADFFEPPFPART
                     AIQVAALPKGGEVEIDVIALR"
     gene            complement(572..1282)
                     /locus_tag="PLJ11_04365"
     CDS             complement(572..1282)
                     /locus_tag="PLJ11_04365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO03938.1"
                     /translation="MSLIMKSALRPNGLRAVIFDMDGTLIQTTIDFHAMNRAVTDTLL
                     RHGLPKAVLDPSGRVNESIARAYAYLHAQGREERAEALELDLNRVTAEVEMARVEESV
                     EVPGAGDVLEGLRSRGMRSAVLTRGSREYTTRALRASGLEGWFDIVVCRDDHPLTEAK
                     PNPLALQRVHSALGLLNRECLFIGDHEIDLRCAKGAGSPFVAVLTGSNDQRAWDRLGA
                     EMVISSVADLPEILERGP"
     gene            1471..1683
                     /locus_tag="PLJ11_04370"
     CDS             1471..1683
                     /locus_tag="PLJ11_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03939.1"
                     /translation="MIKKKVLIGVAALVAFAACALLLGSSIGLTTGIQAIPFIDPHSI
                     PFIDPHAIPFIDPHAIPFIDPHAVAL"
     gene            complement(1709..4366)
                     /locus_tag="PLJ11_04375"
     CDS             complement(1709..4366)
                     /locus_tag="PLJ11_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO03940.1"
                     /translation="MKERVFLHLLFQQRFALDSDSPKSVTQEGVAKAVDVGRNNVAKV
                     LAEMGREGTVETFTRHVKGLPTVRKVYFLSAKGQAVAKAIKQEVEATVVDVIDLQGER
                     HRDEIGRLGNYLPKRYDLLELVNGIVQGTLDCRAFHANRMREERRYVDFTDKKPAVRA
                     FFGRERELDVLAALLTSETKRMVVINGIPGIGKTTLLAKFAQTVRESTNVFWYKVHEW
                     ANLKALLRPMAEFLSQLGRRNLEMYLNQTEAPLVGEAAHILETDLTDVSALLIFDDVH
                     KAEPAVLEFLSALLTIMEGSEHVRAVCTARELPTFYSRSAVFRGLVEELNLEGLDRGS
                     SERMIRARGLAAEDVDEIFRVTNGHPLFLELIDDPKLALGKNIRIFIEQEVFSRLGVA
                     ERKIMSIAAVFRYPVLIDAFFTMEEEIQLAAGMDRVELEGVDYAVSYEAVDSLLSKAI
                     IYESVGGLIGMHDLLREFSLSRLTPRQRRVYHRAAARYYLQDGSAPARVEGLYHSLMA
                     GDLQTAASIAAGDGRTIISRGYATQFAPLLDRLVRDMHEADPKDLVEILLLQGEVLYL
                     QGEWDRAMEVLRSLREGPPGRLDGRMLSEVDRMMGVILLNRADYERADALLQEGLDIA
                     TRSGNRSTTADILYDMGGVMERRGMHSEAVERFQRAREIAIELADDLALGKALYGLGR
                     AYATLRDYERAIAYKKEAMAILERRGDVTMMAKASISIGNDLCILGRNAEGMTFLARA
                     IELANAVGDLSTMGYALSNMAGCKLNQGDLEGAEELVERCLPISTKLDDPLMTSTLLF
                     HRGYIRTSRRQWDAACNDFDRSLEILRGIDMPTRLGQWLIEIAEVYVDHGEVNQARAL
                     LDEAYDIASRIGHQKLLEKVAENRERLCA"
     gene            4520..5080
                     /locus_tag="PLJ11_04380"
     CDS             4520..5080
                     /locus_tag="PLJ11_04380"
                     /inference="COORDINATES: protein motif:HMM:NF017570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF835 domain-containing protein"
                     /protein_id="HOO03941.1"
                     /translation="MQTAEMKEARVKLLMDGYPRKGFSIFSSYLGKERGVCITRLHPE
                     YVREKYGLEGSRFHWLSGVKQEGTLSPKNISALVKQVRGELKGGRCKFFLDGVEYLLL
                     WNDMDSIVASLREIERELERVEGCMIVSIDPLTFEERDLTLLKGAFPEMPVLSAGSPH
                     TDAAEPADKDRIDAGLLVSRGSLATP"
     gene            complement(4980..5402)
                     /locus_tag="PLJ11_04385"
     CDS             complement(4980..5402)
                     /locus_tag="PLJ11_04385"
                     /inference="COORDINATES: protein motif:HMM:NF017570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF835 domain-containing protein"
                     /protein_id="HOO03942.1"
                     /translation="MSERLDKTLSLINALASAGKEIICVSRIHPDLLKERYPLHAIKG
                     VWLSERGNDNCVSPDQLERVVEVIADFVSSRRNAAAVLDGIEHISLFTDFRTLNIFLE
                     ELNDVIMASRAILLIPIDPRLFDPCQLARLRRYAETLH"
BASE COUNT         1063 a         1847 c         1585 g         1156 t
ORIGIN      
        1 cctctcggtg gccaggttca catctgcctc gaagaccccc tccagcccgt tcaacgaatc
       61 ggcgatggtg ccggcgcaag tggcgcaggt catgcccgtc acggagaacg ttgccctctt
      121 ccgcctgccc tccgggctct cctccctttc cccgctcatc gaacgctcaa cccaggtcct
      181 gggtgaaaaa tgttttcaca gacctatcgc aaggcgatca cgtctatctc gacctccccg
      241 cccttcggca gggcggccac ctggatggcc gtcctggcag ggaatggagg ctcgaagaag
      301 tcggcatagg ccttgttgac ctcggggaaa tgggccaggt cggtcatgta caccgtgacc
      361 ttgatcgctc tgtcgaggga gctccccgcc gcctgcagga ccgcctgcag gttcgcaagt
      421 gcctgcctgg cctgcgccac agccccctcg gccatcgctc ccgtagctgg gtcgagcccg
      481 agctgccccg aacagaagac caggtccccg cacacaaccg cctgggaata ggggccaacg
      541 ggggcggggg cgccgtccgc cctcacctcc ctcatggccc cctctccaat atctcaggga
      601 ggtcggccac gctgctaatc accatttctg cgcccaaacg gtcccacgcc cgttggtcgt
      661 tgctccccgt cagcaccgcc acgaacgggc tccccgcccc tttggcgcac cgtaagtcta
      721 tctcatgatc gccaatgaaa aggcattccc ggttcaacag gcccagggcg ctgtgcaccc
      781 tctgtagcgc cagggggttg ggcttggcct ctgtcaaagg atgatcgtcc ctgcaaacga
      841 cgatatcgaa ccagccttcc aggccggagg cacggagcgc gcgcgttgtg tattcccggc
      901 tccctctggt cagcacggcc gagcgcatcc ctcgggacct tagaccctcc aggacatccc
      961 cggcccctgg gacctctacg ctctcctcca ccctggccat ctccacctcg gcggtgaccc
     1021 ggttgaggtc cagctccaag gcttccgccc tctcttcccg accctgggca tggagatagg
     1081 cataggcccg cgcgatgctc tcgttcaccc ttccggaggg atcgagcacg gccttaggaa
     1141 gcccgtgcct cagcaaggtg tccgtgaccg cccggttcat cgcgtggaag tctatggtcg
     1201 tctggatcaa tgtcccgtcc atgtcaaata tgaccgccct aagtccgttc gggcgaaggg
     1261 cgcttttcat tatcaggctc atggtcgaag cctatcagcc ccccttgact attaattatt
     1321 atggcgacct caggtcaaag gttccagtta tttcagacga tatcgttcgc tataatacag
     1381 atattaaaac ttttctgtag catgctcagg gaccgttttt atatgcacaa aaccaagatg
     1441 gttcaaatat aatcaaaggt gccctttatg atgataaaga aaaaagtatt gatcggtgtg
     1501 gcggccttgg tcgccttcgc tgcctgcgca ctgctactcg gatcgtccat tggcctgacg
     1561 accgggatcc aagctatccc gttcatcgat ccccactcga tcccgttcat agacccgcat
     1621 gccatcccgt tcatcgatcc ccatgcaatt ccgttcattg acccgcatgc cgtagccctc
     1681 tgagcgcttg aacgtaaaga agtgcgcctc aggcgcacaa cctttcccta ttctctgcca
     1741 ctttctccag aagtttttga tggcctatcc tgctcgcgat gtcataggcc tcgtccaaaa
     1801 gggcgcgggc ctggttgacc tcgccatggt ccacgtagac ctcggcgatc tctatcagcc
     1861 attgcccaag ccgggtgggc atgtcgatgc ccctgaggat ctccaaggac ctgtcgaaat
     1921 cattgcaggc cgcatcccac tgcctgcggc tggtgcggat gtatccgcgg tggaacagca
     1981 gggtcgaggt catgaggggg tcgtccagct tcgtcgagat tggaaggcag cgctcgacaa
     2041 gctcctccgc tccctccaga tccccttggt tcagcttgca gccggccatg ttcgataggg
     2101 catagcccat ggtgctcagg tcgcccaccg cgttggccag ttcgatcgcc ctggcgagga
     2161 acgtcatccc ctcggcgttc ctgccgagga tgcacaggtc gttcccgatg cttatggagg
     2221 ccttggccat catggtcacg tccccccggc gctccaggat cgccatggcc tccttcttgt
     2281 aggcgatcgc ccgctcatag tcccgcaggg tggcgtaggc cctccccagc ccatagaggg
     2341 ccttgcccag cgccaggtcg tcggccaact ctatggctat ctcgcgtgcc ctctggaagc
     2401 gctcgaccgc ctcagagtgc atcccacgcc gctccatcac ccctcccata tcatacagga
     2461 tatcggccgt ggtggagcgg ttcccgctcc tggtcgcgat gtcgagcccc tcctggagga
     2521 gggcatcggc cctctcgtag tcggcccggt tgaggaggat cacccccatc atgcggtcca
     2581 cctcgctgag catccggcca tccagccttc ccggggggcc ttcccgcagg gacctcagga
     2641 cctccatggc ccggtcccac tcgccctgca gatacaggac ctccccctgc aggaggagga
     2701 tctccacgag gtccttgggg tccgcctcat gcatgtcccg gaccagtcgg tcgagcaacg
     2761 gggcgaactg cgtggcatac cccctgctta taatagtccg cccgtcaccc gcggcgatgg
     2821 aggccgccgt ctgcaggtcg cccgccatca gggagtggta gagcccctcc accctcgccg
     2881 gggccgagcc gtcctgcagg tagtagcggg cggccgccct gtggtacacc ctcctctgcc
     2941 tgggcgtgag ccgcgacagg gaaaactcgc gaaggaggtc gtgcatgccg atcagccccc
     3001 ccaccgactc gtagattatg gccttggaga gcaaggagtc caccgcctcg tagctgaccg
     3061 cgtaatcgac cccctccagc tccacccggt ccatgcccgc ggcgagctgg atctcctctt
     3121 ccatcgtgaa gaaggcgtcg atgagcaccg ggtatcggaa caccgcggcg atgctcatga
     3181 tcttgcgctc ggccaccccc agcctggaga acacctcctg ctcgatgaat atccttatgt
     3241 tctttcccag ggcaagcttg gggtcgtcga tgagctccag gaacaacgga tggccgttgg
     3301 tcacccggaa gatctcgtcc acgtcctcgg cggcaaggcc ccgggcgcgg atcatgcgtt
     3361 cgctgctccc tcgatccagc ccttccaggt tcagctcctc caccagcccg cggaacacgg
     3421 cgctgcggga atagaatgtg ggaagctccc tggcggtgca caccgcccgt acatgctccg
     3481 atccttccat tatcgtcagc agggccgaga ggaactccag gaccgccggc tcggccttgt
     3541 gcacgtcatc gaatatcaga agggctgaga cgtccgtaag gtccgtttcc aggatatggg
     3601 cggcctcgcc gaccaggggg gcctcggtct ggttcaggta catctcgagg ttcctgcgtc
     3661 ccagctggga caggaactcg gccattggcc tgagcagcgc cttcaggttc gcccactcat
     3721 gcaccttgta ccagaagacg ttggtgctct ccctgaccgt ctgggcgaac ttggccaaca
     3781 atgtggtctt tccgatcccc gggatcccat tgattaccac catccgcttg gtctccgagg
     3841 tcagaagggc ggccaggaca tccaattccc tttcccggcc gaaaaaggcc ctgaccgccg
     3901 gcttcttgtc cgtaaagtcc acgtaccgcc gctcctcccg catgcggttc gcatggaagg
     3961 cacggcagtc tagggtcccc tggacaatgc cattgaccag ctcaagcagg tcataccgct
     4021 tcgggaggta gttgcccagc ctgcctatct catcccggtg gcgctcgccc tgcaggtcga
     4081 tgacgtccac cacagtggcc tccacctcct gcttgatcgc cttcgccacc gcctggcctt
     4141 tggcgctcag gaagtacacc ttgcgcacgg tggggagccc cttaacatgc ctggtgaagg
     4201 tctccaccgt cccttccctg cccatctccg ccaggacctt ggccacgttg ttccttccga
     4261 cgtccaccgc cttcgctacc ccttcctggg tgaccgactt cggagagtcc gagtcgaggg
     4321 cgaagcgctg ctggaacagc agatgcagaa acaccctctc cttcaccgta agcaacgacc
     4381 ttcccggacc ttggttcaca gataaaaatc acctttccca tatcaatatt cttccactcc
     4441 cctgtttgct cattcggcga aagtagcatg ctcaaattcc ctttaaatac cgttaaaacg
     4501 aaaatagggg gacggaggaa tgcaaacggc agaaatgaag gaggctaggg tcaagctgtt
     4561 gatggatggg tatccccgga aggggttctc gatcttctcg agctacctcg gaaaggagcg
     4621 gggggtgtgc atcacccgcc ttcacccgga gtatgtgagg gagaagtacg ggctggaggg
     4681 ctcgaggttc cattggttga gtggggtaaa gcaggaaggc accctttccc cgaagaacat
     4741 ctctgctctg gtcaaacagg tccgggggga gctcaagggc ggcaggtgca agttcttcct
     4801 cgatggcgtg gagtacctcc tcctttggaa cgacatggac agcatcgtgg cctcgctgag
     4861 ggagatagaa agggagctgg agagggtgga gggatgtatg atcgtctcca tcgacccgct
     4921 gaccttcgaa gagagggacc tgaccctgct gaagggggcg ttccccgaga tgccagtgct
     4981 cagtgcaggg tctccgcata ccgacgcagc cgagccagct gacaaggatc gaatagacgc
     5041 gggtctattg gtatcaagag gatcgctcgc gacgccatga tcacgtcgtt cagctcctcc
     5101 aggaagatgt tcagggtccg gaagtcggtg aagagggaga tgtgctcgat cccgtccagc
     5161 accgccgccg cgttccgtcg ggacgatacg aagtccgcta tgacctccac taccctttcc
     5221 agttggtccg gggacacgca gttgtcgttc cccctctcgc tcagccaaac ccctttgatg
     5281 gcatgcaaag gataacgttc cttgagcagg tccgggtgga tgcggctcac gcatatgatc
     5341 tccttccccg cggaggccaa ggcgtttatc agggagaggg tcttgtcaag ccgctcgctc
     5401 attatgatgt agatgcgccc ttgatcgagg gtgaacttcc cgtagtccga atggacatcg
     5461 acggaactat aaccgttccc cgggtctagg ccagcgctcg tagcatcagt ccccgcatca
     5521 gataataggg gtatgagatt aataatggta ttcaaaagaa tttgaccata tccgttggaa
     5581 ggaatgtgca aaacggttaa aaaaaagaag gggggttagc cccttgaggc acctacgtca
     5641 ggttcccgct c
//
LOCUS       DAOSYN010000078         5530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_12355_length_5530_cov_4.361096, whole genome shotgun sequence.
ACCESSION   DAOSYN010000078 DAOSYN010000000
VERSION     DAOSYN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12355_length_5530_cov_4.361096"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLJ11_04390"
     CDS             complement(<1..681)
                     /locus_tag="PLJ11_04390"
                     /inference="COORDINATES: protein motif:HMM:NF026161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030218 - erythrocyte differentiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPD domain-containing protein"
                     /protein_id="HOO03943.1"
                     /translation="MKNEDFKYLYDRLKEPSDIDALSKDLCLDREFLTVIYTQRTVRE
                     TTKKYYKVQRNGRRMVRSWQSGESILEIARRYEFPPILTGLMIFQEAGRSKKQFWSLV
                     RDPESIQDPDLRTQIEEIIAADIIYSPEGTEKQYQRGQWGEKNLQDWLTSQGIGFRTE
                     KDLRGEYPKTPDALLDRPILVNGWKVNWIESKATFGDRVEVNKNTRKQLSPYVELFGQ
                     GLVVYWFGF"
     gene            839..1456
                     /locus_tag="PLJ11_04395"
     CDS             839..1456
                     /locus_tag="PLJ11_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03944.1"
                     /translation="MDLSDFLASENGPAWLPASVAEARKLVYIGQLFALIFSAIALLA
                     GIIAIISGEFGGGIYSLVAAGLGVVVLLMVKGNVFANLDQGRFKQASDALLVWTIVGF
                     FVYIIPGLIMLFGFLKLQEIFQPQYQQYQAQPAQSVPAPEQPSPVPPASAPEPPAPQP
                     QSVPPEGAPVQEHKEKKVEMTKCRKCGVNYPAFMHNCPNCNEPRH"
     gene            complement(1416..1685)
                     /locus_tag="PLJ11_04400"
     CDS             complement(1416..1685)
                     /locus_tag="PLJ11_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair domain-containing protein"
                     /protein_id="HOO03945.1"
                     /translation="MTFPREALNRLRWGAGGLDGVVVTYIHRGAPGGLASVMGEDITE
                     LGRSFFVVGEAHIPYHRIVLIERGGEIVFRAPPNGGAHCSSGNCA"
     gene            1745..3370
                     /gene="ade"
                     /locus_tag="PLJ11_04405"
     CDS             1745..3370
                     /gene="ade"
                     /locus_tag="PLJ11_04405"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOO03946.1"
                     /translation="MGRTEIIGQLVDVIEGGIAPAKVVMEEGRFTEVAPLPEAPRRFL
                     LPGLIDSHIHIESSQLCPSRFAEAVVPHGTTAVVSDPHEIANVLGMKGIEYMLEDSSK
                     VPLRIYLTAPSCVPATPFERSGAAIGVAEIEFMLKDPRFVGLGEVMDVQGVLSDDPRI
                     ISKIKAAKAFWKGIDGHCPGLTGTDLVKYINAGIRTDHESLTAEEAEEKYFLGMWVQV
                     REGSASQDLRTLMPFAKGHECMLVSDDLRAEDIVNGHMDVLLRKAVALGMPPLHAIRA
                     VTAWPAWHYGLAGGSVAVGRTADLVVVDDLHSFNVLEVYIGGTKVAERGTALFTATPR
                     ANGLGISPRALHGQDLLLEAKGERAAVRAIEAYPNRIQSGGLVTELPVREGRVRASLE
                     QDVLHLALVNRYLDERPVLGFIKGFGLKRGAMATSVSHDCHHLLAVGASVEDMAMAIN
                     AVSRAGGFAVCDNGKMKTLPLEVAGLMSVSPVGTVAQQEAHLLAMLNEMGCRLPAPFM
                     TLSFQSLLVVPELKIGDRGLFDTVRMEFVPAVL"
     gene            3427..4113
                     /locus_tag="PLJ11_04410"
     CDS             3427..4113
                     /locus_tag="PLJ11_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOO03947.1"
                     /translation="MDKVESFKCRILTWDEIAHWTEAVAYDIADNGFRPTVVIGLTRG
                     GWVPARVICDHLQVKRLYAVKTEHWGVTANHDGRALLTQELNASIEGESVLIVDDITD
                     TGESLMLAEKHLGQMGPKEVRTATLLHITHSKKRPDYYEVEVPEEDWTWFIFPWNLHE
                     DLRTLLPKTLHTPQTVLGIQKGFKEQFQIDVPEDLVRRTIRHLAASGKIRKEGAKWHR
                     VDAGEALVPK"
     gene            4341..4898
                     /gene="hpt"
                     /locus_tag="PLJ11_04415"
     CDS             4341..4898
                     /gene="hpt"
                     /locus_tag="PLJ11_04415"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine/guanine phosphoribosyltransferase"
                     /protein_id="HOO03948.1"
                     /translation="MLERLKESLERSDVVRFGEYQYFVHPITDGIPAMDPRVLEEVLD
                     RIFDMMPMDVDYLVGAEAMAIPLIVPLSLMTGIPYNVVRKRRYGLPGEVSVRQTTGYS
                     EKELFINGLRKGDRVIVMDDVVSTGGTLRAIVKALRAMGVEVADIIVVIRKTDRLEEL
                     ERDIRQKVRTLVSVEVRDGRVHVLP"
     gene            5151..>5530
                     /locus_tag="PLJ11_04420"
     CDS             5151..>5530
                     /locus_tag="PLJ11_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO03949.1"
                     /translation="MLGADLLLLLYFTLFGMGLGALSGLAPGIHVNTLAALLMIGAPA
                     LIAPLEGLCLSVGCALSPALLLACAILSAAVCHSFLDYIPSLIMGVPDESECLSVLPS
                     HRLLLEGKGMNALQAAAQGSLVGA"
BASE COUNT         1018 a         1737 c         1748 g         1027 t
ORIGIN      
        1 gaagccgaac cagtagacga ccaggccttg gccgaacagc tccacatagg gggagagctg
       61 cttcctggtg ttcttgttga cctccacgcg gtccccgaag gtggccttgg actctatcca
      121 atttaccttc catccattga ccaggatggg tcggtccaac agggcatccg gggtcttggg
      181 gtactccccg cgcaggtcct tctccgttcg gaacccgatc ccctgggagg tcagccagtc
      241 ctgcaggttc ttctctcccc actgtccacg ctggtactgc ttctcggtcc cctccgggga
      301 gtagatgatg tcagccgcga ttatctcctc tatctgcgtg cgcagatcgg ggtcctgaat
      361 gctctctggg tccctcacaa ggctccagaa ctgcttcttg gaccgtcccg cctcttgaaa
      421 gatcatcagg ccggtcagga tgggcgggaa ctcatagcgg cgcgctatct ccaggatgga
      481 ctccccgctc tgccaggacc tgaccatcct ccttccgttc cgctggacct tgtagtattt
      541 tttggttgtc tcccgcaccg tccgctgggt gtagatgacg gtaaggaact cgcggtccaa
      601 gcagagatcc ttggagagcg catcgatatc agagggttcc ttcaatcggt catacaggta
      661 tttgaaatcc tcattcttca tccatttcaa ccttggcgcc ttttgtagac ataggaacat
      721 atataattgt tatggggttg gcgcccctcg cgggccgcgt ctctaagggc tcgatagatt
      781 aaaatccgcg aatcccatgt atgagatcca ttgggatgga gccctctgga ggaacaccat
      841 ggacctgagc gatttcctgg catcggagaa cggccccgcc tggctgccgg ccagtgtggc
      901 ggaggcccgt aagctcgtct acatcggtca attgttcgcc ctgatcttct cggccattgc
      961 gttgttggcg gggatcatcg ccatcatcag cggtgagttc ggagggggca tctactccct
     1021 ggtggcggcc ggcctgggcg tggtggtcct gctgatggtc aaaggaaacg tgttcgccaa
     1081 cctggaccag ggcaggttca agcaggccag cgatgccctg ttggtctgga ccatcgtagg
     1141 gttcttcgtc tacatcatcc ccgggctgat catgctgttc ggcttcctca agctgcagga
     1201 gatcttccag cctcaatacc agcaatatca ggcacagccc gctcaatcgg tcccggcccc
     1261 tgagcagccc tcccccgtcc cgcccgcttc agcccctgag ccccccgccc cgcaaccgca
     1321 atcggtccca cccgagggag ctcccgtcca ggagcacaag gagaagaagg tggagatgac
     1381 caagtgtcgg aagtgcggcg tcaactaccc cgccttcatg cacaattgcc cgaactgcaa
     1441 tgagccccgc cattaggcgg ggcccggaag acgatctccc cgcccctctc gatcagcacg
     1501 atgcgatgat aagggatatg cgcctccccc acgacgaaga acgaccttcc cagttccgta
     1561 atgtcctctc ccatgaccga ggccaggccc cccggggccc cccggtggat ataggtgacc
     1621 acgaccccat ccaggccccc ggccccccac cgcagcctgt tcagcgcctc cctgggaaag
     1681 gtcataggcg aacatcggat ggaaggggca aaagattttt gcatgttaaa gctgaatcgc
     1741 acccatgggc aggacggaga tcataggcca gctcgtggac gtgatcgagg ggggcatagc
     1801 ccccgccaaa gtggtcatgg aggagggcag gttcacagag gtggccccgc tgccagaggc
     1861 gcccaggcgg ttcctcttgc cggggctgat cgattcccac atccacatcg agtcctctca
     1921 gctctgccct tcccggttcg ccgaggcggt ggtgccccac ggcaccacag cggtggtctc
     1981 ggacccgcac gagatcgcca atgtgctggg gatgaagggc atcgagtaca tgctggagga
     2041 ttcatccaag gtgccactgc gcatctacct caccgccccc tcatgcgtgc ctgccacccc
     2101 cttcgagcgc tccggggcgg cgatcggcgt ggcagagatc gagttcatgc tcaaggaccc
     2161 ccgcttcgtt ggtctggggg aggtcatgga cgtccaaggg gtcctcagcg acgacccgcg
     2221 catcatttcg aagatcaagg cggccaaggc gttctggaag ggcatcgacg ggcattgccc
     2281 cggcctgaca ggcaccgatc tggtgaagta catcaatgcg gggatccgaa cggaccatga
     2341 gagtctcacg gccgaggagg ccgaggagaa gtacttcctg gggatgtggg tccaggtgcg
     2401 ggaggggagc gcctcccagg acctgcgaac gctcatgccc ttcgccaagg gccacgagtg
     2461 catgctggtc tccgacgacc tcagggcgga ggacattgtc aacgggcata tggacgtcct
     2521 gctcaggaag gcggtggccc tggggatgcc ccccctgcac gcgatcagag cggtcaccgc
     2581 ctggccagcc tggcattacg gattggccgg ggggagcgtc gccgtgggcc gcaccgccga
     2641 cctcgtggtg gtcgacgacc tacacagctt caacgtcctt gaggtgtaca taggcggaac
     2701 taaggtcgcg gagcgcggca ccgccctctt cacggcgacg ccccgggcca atggccttgg
     2761 gatctcgcct agggccctgc acggccagga cctcctgctc gaggccaagg gggaaagggc
     2821 agccgtcagg gcgatcgagg cctatccgaa ccggatccag agcggcgggc tggtcaccga
     2881 gctgcccgta cgcgagggac gtgtccgagc ctcactggag caggacgtgc tccacctggc
     2941 cttggtgaac cgctacctgg acgagcggcc ggtgctgggc ttcatcaagg gtttcggcct
     3001 gaagcggggg gccatggcaa ccagcgtctc gcacgattgc caccacctcc tagcggtcgg
     3061 ggcgtcggtc gaggacatgg ccatggccat caatgccgtg tcccgggccg gtgggttcgc
     3121 tgtgtgcgac aacggaaaaa tgaaaacgct tcccttggag gtggccgggc tcatgagcgt
     3181 ctcccctgtg ggtaccgtcg cgcagcagga agcccatctg ctggccatgc tcaatgaaat
     3241 ggggtgcagg cttcccgccc ccttcatgac cctctcgttc caatcgctcc tggtcgtgcc
     3301 ggagctcaag ataggggacc gcggattgtt cgacaccgtg cgcatggagt tcgttcccgc
     3361 ggtcctctga aggagaagcg ttatcttccc gtagagcggt caagggccgt taccacgggg
     3421 ggtatcatgg acaaggtgga gagcttcaag tgccggatat tgacctggga cgagatcgct
     3481 cattggaccg aggcggtggc gtacgacatc gctgacaacg gcttccgccc gacggtcgtc
     3541 atcgggctca cccgcggagg gtgggtgccg gccagggtga tctgcgacca tctgcaggtg
     3601 aagaggctgt acgcggtgaa gaccgagcac tggggggtga ccgccaacca cgacgggagg
     3661 gcgttgctca cccaggagct taacgcgagc attgagggcg aatcggtcct gatcgtggac
     3721 gacatcaccg acacggggga gagcctcatg ctggccgaga agcacctggg gcagatgggg
     3781 cccaaggagg tccgcacggc cacgctcctc cacatcaccc attccaagaa gcgccccgac
     3841 tattacgagg tggaggtgcc agaggaggac tggacatggt tcatcttccc ctggaacctt
     3901 catgaggacc ttcgtaccct gctcccgaag acgctgcata cgccgcagac ggtgctgggc
     3961 atccagaagg gcttcaagga gcagttccag atagacgtcc ctgaggacct cgtccgtcgc
     4021 accataaggc atctggccgc ttccgggaag atcaggaagg aaggggcgaa gtggcacagg
     4081 gtcgatgccg gggaggccct cgtcccgaag tagggccgag gacgcacagg gcgttcaatg
     4141 ccatcggacg accagggcat cggggcgcaa ccgcggcgat caccgtctcc tcaatcccgg
     4201 gcctcgggaa gggccttggg ggaacggtcc cctgtggaga cggttcttca tacccgtggt
     4261 cctcccggga atcgcttgga ggggtcgtct tgacgggcgc atgattaatt ggtggccggg
     4321 gcatataccg cccgaggcgc atgctggaga gattgaagga aagcctggag cgcagcgatg
     4381 tggtgcgctt cggcgaatac caatatttcg tgcatcccat caccgacggc atcccagcca
     4441 tggaccccag ggtcctcgag gaggtgctag accgcatctt cgacatgatg cccatggacg
     4501 tggactacct cgtgggagcg gaggccatgg ccatcccgct catcgtgccc ctctcgctga
     4561 tgaccgggat cccgtacaat gtggtgcgca agcgcaggta cggccttcca ggggaggtca
     4621 gtgtccgcca gaccactggg tactcggaga aggagctgtt catcaacggg ctgaggaagg
     4681 gtgaccgcgt catcgtcatg gacgacgtgg tgagcaccgg cggcacgctc cgggctatcg
     4741 tgaaggcgct cagggccatg ggcgtggagg tggccgacat catagtggtg atcaggaaga
     4801 ccgacaggct cgaggagctg gaaagggata tccgccagaa ggtcaggacc ctggtgagcg
     4861 tagaggtgag ggacggccgg gtccatgtgc ttccctgaac cggcgtccca ccgcacgtcg
     4921 gcgggaacgt gatcggggcc ggggtcgcaa tgcgcgctgt ccctcatcaa ggccgagaca
     4981 ttcccttccc tgttcaatga tgagcggtcc cccaccccgg cccccgcgag gtcctccctc
     5041 tgcctaggaa gggcatgcat gccgcacaac gacatatgaa cctcggcgca gagcacggta
     5101 aggctcactg caaccgttcc cctattcatc ccacgctcaa ctggtaccat atgctcggtg
     5161 cggacctcct gctcctgctc tacttcaccc tattcgggat gggcctcggg gcgttgtccg
     5221 gcctggcgcc cggaatacat gtgaacactc tcgccgccct cctgatgatc ggtgcccccg
     5281 ccctgatcgc ccctcttgag gggctgtgcc tctcggtggg ctgcgcgctc tccccggccc
     5341 tgctccttgc ctgcgcgatc ctgtcggcgg cggtctgtca ctccttcctg gactacatac
     5401 cgtcgctgat catgggcgta ccggacgaat cggagtgcct ctccgtcctc ccctcccacc
     5461 gcctgctgct ggagggaaag gggatgaacg ccctgcaggc ggccgcccag ggaagcctgg
     5521 tgggagccat
//
LOCUS       DAOSYN010000079         5483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_12493_length_5483_cov_4.430730, whole genome shotgun sequence.
ACCESSION   DAOSYN010000079 DAOSYN010000000
VERSION     DAOSYN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12493_length_5483_cov_4.430730"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..369)
                     /locus_tag="PLJ11_04425"
     CDS             complement(235..369)
                     /locus_tag="PLJ11_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03950.1"
                     /translation="MEGSEMNDVYGLIYWIIVGTMVLAEIAAGYLTYRHLEEADRCRR
                     "
     gene            complement(439..1062)
                     /locus_tag="PLJ11_04430"
     CDS             complement(439..1062)
                     /locus_tag="PLJ11_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03951.1"
                     /translation="MDEIHRRQFLAWSRDDDHLAKLDRTRREIAACPDRSTVLFSGGK
                     DSLTVLDLAVKAGKCGQAIHIHSGYDLMPKPYYDEIKEQLRRVCGDRCRIVLIEPGGK
                     DDPDPIPFVIGTLRAEENGRRRRGGPNADCFGAPCRQVIFDWSWMDVWAYIVSNDLPY
                     HKVYDLRAPIEGWDKARFSEFFCKDLERFTGAVDGLTAWRYRPQTRR"
     gene            complement(1046..1351)
                     /locus_tag="PLJ11_04435"
     CDS             complement(1046..1351)
                     /locus_tag="PLJ11_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03952.1"
                     /translation="MPAAGEWFITPHAIEKYRERFAPYLTYEEALEDLIEQSRKAHYV
                     KTYDHHPNSIRGGLELWRGPRPRRLRYWVGLDNDGKPQLVTVIGEHDRWRYGDGRDP"
     gene            complement(1354..2157)
                     /locus_tag="PLJ11_04440"
     CDS             complement(1354..2157)
                     /locus_tag="PLJ11_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03953.1"
                     /translation="MNKWEIERALSEYRPPNPFSDFSVTFRMRTQIALANPWINGDAL
                     VMYLLLNDLLGDAYYDLPRMPLDLRDLRLPLERTHEDLVPGGIHHASASLFDGEFLTN
                     MAPDRPGIFSTTQYKRFASEQLDHLNSSKKIPLDVGYFKAMMTTTPYVHAREVKFYMR
                     GDRQECERLLKHVTHLGKDRGRGWGEVMGMTIEELPEDRSLEYNGVAMRPLPWPMVEG
                     RVRQQDLANIAYRTPYWVRSNTMLCAPPGCALSTKAPNDDVDWYAQAPG"
     gene            complement(2154..3017)
                     /locus_tag="PLJ11_04445"
     CDS             complement(2154..3017)
                     /locus_tag="PLJ11_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03954.1"
                     /translation="METQTYRGYLIAKSPLHHGGDEQSGATALVRRHKYLSSDGRVLN
                     VPVISGNSIRGNMRRMVMGDMLYRIGYGELKNAKVYHMLFAGGSLESVDVGDSGKIDV
                     GMKKKYRLGIPPLGLFGTSIGNQMIEGRLKVGHAIPLCKEIAPMLAPDGEVRQLADLK
                     DIISSARQRSYYELLGHDFQTRRDDLHEEREEGEAAHQMKVDLETVAMGTVFEHTFAL
                     WNTSPIEAAVLGRAMELWRTRPYVGGKSGSGYGRVGIYYDVPDQAPWLNYMREHADDL
                     KTIIADLEATI"
     gene            complement(3021..3359)
                     /locus_tag="PLJ11_04450"
     CDS             complement(3021..3359)
                     /locus_tag="PLJ11_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03955.1"
                     /translation="MNEERMQVYEELSSILSLAWSRVEWRRITSMKRTALDIFTDRIR
                     AAASEPDVVTAIERLCKLLGISSINPEPDAVRAIMARNDEAMHAMRENAIYLAVLSKE
                     KAKAKYAKEG"
     gene            complement(3331..4023)
                     /locus_tag="PLJ11_04455"
     CDS             complement(3331..4023)
                     /locus_tag="PLJ11_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03956.1"
                     /translation="MIDDGPIIKTVPELVCLARGIVPELGTLRGICVYCQRETEHGHK
                     HKDNADSCATYHLIRGGTVVCPSCQHMRGERWLYRSNMWEASASGFRTFKFQEARDAL
                     LNPPEPPFAQFFTKTWKKNGWMNMVNEVNWSRDRYTVALDYDIIHVIAERRDRMIALA
                     EGLLAKGARKADLVSGELGPKAVLALGADTLSSMAEVRRYASDPLWDLVVYVAHGPKK
                     EAKDERGENAGV"
     gene            complement(4020..4451)
                     /locus_tag="PLJ11_04460"
     CDS             complement(4020..4451)
                     /locus_tag="PLJ11_04460"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HOO03957.1"
                     /translation="MTAIKFFVAGEPAPKGSTKSFPYRCKDGRIGVSTTNANTRTAPW
                     EMRIATEAQKAMAGRAPFEGPVYIEAGFWLTRPKSLPKRVTLPIGRKNDLDKLLRCCL
                     DGLTGICYLDDGQVAIISGSYKCYANHDHPPGCAITVGEVL"
     gene            complement(4451..4747)
                     /locus_tag="PLJ11_04465"
     CDS             complement(4451..4747)
                     /locus_tag="PLJ11_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOO03958.1"
                     /translation="MEMVMMIDERIGTADPHNERESEAAAESGGHEIVPGEDRDDEIV
                     ALHNQGLTVIEIGKRLGWPTQKVSHRVRMCRKYGLIEKRTDTERRVRTLWPGGR"
     gene            complement(4722..4967)
                     /locus_tag="PLJ11_04470"
     CDS             complement(4722..4967)
                     /locus_tag="PLJ11_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03959.1"
                     /translation="MKTARDRERLMLLIGLEKGTYSVPQLMQSWLAFYGPRSAPTRGQ
                     IGAMLRQLKGKGIVEMVGEGCPQLWRTAKNGDGNDDR"
     gene            complement(4964..5149)
                     /locus_tag="PLJ11_04475"
     CDS             complement(4964..5149)
                     /locus_tag="PLJ11_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03960.1"
                     /translation="MPTYINYGIVVSIDVERIPELKALLEDLGAKLIYQKASSNHLII
                     AERQKPNDRGSYGEDDR"
BASE COUNT          961 a         1801 c         1493 g         1228 t
ORIGIN      
        1 gttcagacgc tcgatcttaa tgcgatagat atctgtcaat ccgtcccccc gcccttcgga
       61 tcgtactcaa cgatgatctg cccgttgggt gtgggcgtag cggtccgatc atcctcgccc
      121 gagcccaata ggggccgccc gcatcgagga cagctcccac cggtggtaga ttctacccat
      181 ccacacattg gacaatagac gctcatggac cgcctcctgt cataatgcgc gaagtcatcg
      241 ccggcatcgg tccgcctcct cgaggtgtct gtaggtcaga tatcccgccg cgatctcggc
      301 caggaccatc gttccgacga tgatccaata gatcagacca tagacatcgt tcatttcgct
      361 tccttccatt gatctcatgc cgacttcttc ttgacaacgc ttgccatctt cctattcgtg
      421 atcggacttg ccatctcctc acctcctcgt ctgcggtcta tatctccacg cggtcaggcc
      481 gtccaccgct ccggtgaatc tctccaggtc cttgcagaag aactcgctga agcgcgcctt
      541 gtcccatccc tctataggcg ctcgcaggtc atagaccttg tggtagggaa ggtcgttcga
      601 gacgatgtat gcccacacat ccatccaact ccagtcgaat atgacctggc ggcacggggc
      661 tccgaagcag tcggcgttcg gacctcccct cctcctccgc ccgttctcct cagcgcgaag
      721 cgtgccgatc acgaacggga tgggatccgg gtcgtccttg ccgcccggct cgatcagcac
      781 gatccggcag cgatcgccgc acacccggcg cagctgctcc ttgatctcgt catagtacgg
      841 cttcggcatc aggtcgtagc cgctgtgtat gtggatcgcc tggccgcatt tgccggcctt
      901 gacggcaagg tccagcaccg tcagcgagtc cttgccgcct gagaacagca ccgttgatcg
      961 gtcggggcag gcggcgatct ccctgcgggt cctatccagc ttggcaaggt gatcgtcatc
     1021 cctcgaccac gccagaaact gacgcctatg gatctcgtcc atctccgtac ctccatcggt
     1081 catgctcgcc gatgaccgtc accagctgcg gcttgccgtc gttgtcaagt ccgacccaat
     1141 agcgcagccg gcgcggccgt ggacccctcc atagctccag cccgccgcgg atgctgttcg
     1201 gatgatgatc gtacgtcttg acgtagtgcg ccttccgcga ttgctcgatg aggtcctcga
     1261 gcgcctcctc gtacgtcaga tagggcgcga accgctccct gtacttctcg atcgcgtgcg
     1321 gcgtgatgaa ccactcaccg gcggcaggca tggtcacccc ggcgcttgcg cgtaccagtc
     1381 cacgtcatca ttcggagcct tagtgcttag ggcgcatcct ggcggggcgc acagcatggt
     1441 gttgctgcgc acccaatagg gcgtccgata ggcgatgttg gccaggtcct gctgccgcac
     1501 tctgccctcg accatcggcc acggcagcgg gcgcatcgcc acgccattat attccaggga
     1561 ccggtcctcc ggcagttcct cgatggtcat ccccatcacc tcgccccatc ctcgaccgcg
     1621 gtccttgccc agatgggtca catgcttgag cagccgttcg cattcctgtc gatcgccgcg
     1681 catgtagaac ttcacctccc tggcatggac atacggcgtg gtcgtcatca ttgccttgaa
     1741 gtagccgaca tccagtggga tcttcttgct gctattgaga tgatccagct gttccgaggc
     1801 gaatcgcttg tattgggtcg tcgagaatat ccccgggcgg tccggggcca tgttggtcaa
     1861 gaactcgccg tcgaacagcg aggcggacgc atgatggatg ccgcccggga ccaggtcctc
     1921 atgcgtgcgt tccagcggca ggcggaggtc gcgcaggtcc agcggcatcc tcggcagatc
     1981 gtaataggca tcgccaagga gatcgttgag cagcagatac atgaccagtg catctccgtt
     2041 gatccaggga ttggccaggg cgatctgcgt ccgcatccgg aaggtgacgg agaaatcgct
     2101 gaaggggttt ggagggcggt actccgacag cgcccgctcg atctcccact tgttcatatc
     2161 gttgcctcta ggtcggcgat gatggtcttg agatcgtcgg cgtgctcgcg catatagttc
     2221 agccacggag cctgatccgg gacgtcgtaa tagatgccga cacgcccgta cccactgccg
     2281 gacttgccgc cgacgtacgg cctggtccgc cacagctcca tggctcggcc aagcacggcg
     2341 gcctcgatcg gcgacgtgtt ccacagcgcg aacgtgtgct cgaacaccgt gcccatcgcg
     2401 accgtctcaa ggtccacctt catctgatgc gccgcctctc cctcctcgcg ctcctcatgc
     2461 aggtcgtccc ggcgcgtctg gaagtcgtgc ccgagcagct catagtacga gcgctgcctg
     2521 gcgctcgata tgatatcctt gagatcggcc agctgccgga cctctccgtc cggggcgagc
     2581 atcggagcga tctccttgca tagagggatg gcatgcccga ccttcaggcg gccctcgatc
     2641 atctgattgc cgatgctcgt gccgaacagg ccgagcggcg gtatgcccag ccgatacttc
     2701 ttcttcatgc cgacgtcgat cttgccgctg tccccgacat ccacgctctc cagcgagccg
     2761 ccggcgaaca gcatatgata gaccttggcg ttcttcagct ccccgtagcc gatgcggtag
     2821 agcatgtcgc ccatgaccat ccgcctcatg ttgccccgga tgctgttgcc cgagatcacc
     2881 ggcacgttga gcactcgacc gtccgatgac aagtacttat gccgccgcac cagcgccgtc
     2941 gctccgctct gctcatcccc tccatgatgg agagggctct tggctatcag atagcccctg
     3001 tacgtctgtg tttccatgga tcatccctcc ttcgcatatt tcgctttggc cttctccttg
     3061 ctcaggaccg ccaggtagat ggcgttctcc ctcatcgcat gcatcgcctc gtcgtttcga
     3121 gccatgatcg cccgaacggc gtccggctca gggttgatcg acgagatccc gagcagcttg
     3181 catagccgct cgatcgccgt caccacatcc ggctcggatg ccgccgccct gatccggtcg
     3241 gtgaatatgt ccagggcggt gcgcttcatg ctcgtgatcc gtctccactc caccctggac
     3301 cacgccaggg aaagtatgga ggacagttcc tcatacacct gcattctctc ctcgttcatc
     3361 cttcgcctcc ttcttcggac cgtgcgcgac gtaaacgacg aggtcccaca ggggatcgct
     3421 cgcgtaccgc cgcacctcgg ccatcgatga cagcgtatcg gccccgagcg cgagcacggc
     3481 ctttggccca agctcgcccg acacaaggtc ggccttgcga gcgcccttgg ccagcagccc
     3541 ctcggccagc gcgatcatcc ggtcacgcct ctcggcgatg acgtgtatga tgtcgtagtc
     3601 cagcgccacc gtgtagcggt ccctggacca gttcacctcg ttgaccatat tcatccagcc
     3661 gttcttcttc cacgttttcg tgaaaaattg cgcgaacggg ggctctggcg ggttcagcaa
     3721 tgcgtcccgg gcctcctgaa acttaaatgt gcggaacccc gatgccgacg cctcccacat
     3781 gttggaccgg tacagccatc gctcgcccct catgtgctgg catgatgggc agacgacggt
     3841 cccgccacgg atcagatgat aggtggcgca gctatccgca ttgtccttat gcttgtgccc
     3901 atgctcggtc tcgcgctggc aatagacgca tatgcctctc aaggtcccca gctcggggac
     3961 gatcccgcga gcgaggcaga ctagctcggg gacggtcttg atgatcggcc catcatcgat
     4021 cataggacct cccccacggt gatcgcgcat ccgggcgggt ggtcatggtt cgcgtaacac
     4081 ttgtacgagc cgctgatgat cgccacctga ccatcgtcga ggtagcagat gccggtgagc
     4141 ccgtccagac agcagcggag cagcttatcc agatcgttct tgcgaccgat cgggagcgtc
     4201 acacgcttgg ggagggactt agggcgggtc agccagaacc ctgcctcgat atagaccggc
     4261 ccctcgaacg gggcgcgacc ggccattgcc ttctgcgcct cggtcgcgat ccgcatctcc
     4321 caaggagcgg tcctcgtgtt agcgttggtg gtgctgacgc cgatcctccc gtccttgcag
     4381 cggtagggga acgacttggt gctgccctta ggagcaggct caccggcgac gaagaatttg
     4441 atcgcggtca ttatctccct ccgggccaca gcgtcctgac ccttctctca gtgtctgttc
     4501 gtttctcgat cagaccatat ttccggcaca tcctcacgcg atggcttacc ttctgggtcg
     4561 gccatcctag ccgcttcccg atctcgatca cggtcaatcc ctggttatgc aatgcgacga
     4621 tctcatcgtc ccggtcttct ccaggaacta tctcgtgccc gccgctctcc gcggcggcct
     4681 cgctctcgcg ctcattgtgc ggatcagctg ttccgattcg ctcatcgatc atcattacca
     4741 tctccattct tggcggtcct ccatagctgt ggacatcctt ccccgaccat ctcgacgatc
     4801 cccttcccct tgagctgcct gagcatggct ccgatctgcc ctcgcgtagg ggcgctcctt
     4861 ggaccataga atgccagcca gctctgcatg agctggggga ccgaatacgt ccccttctcg
     4921 agcccgatca gcagcatgag gcgctcgcgg tccctggcgg ttttcatcga tcgtcctctc
     4981 catacgaccc tcggtcgttc ggcttctgac gctcagcgat gatgagatgg ttgctcgacg
     5041 ccttctgata gatcagcttc gcgcccaagt cctcgagcaa tgccttgagt tcggggatcc
     5101 gctcaacatc gatggacacc acgatcccgt aattgatgta tgtcggcatc attgaccaaa
     5161 acactcccca tgaagatatt ctgatggtcc ggcgatctta tggaccagaa cgcttgagct
     5221 ttgatatttt atcatcattt tattcgtcct caccgaggcg cgatcgtgtc gcggtcccga
     5281 tgtgttgatg atatcggaat tgttgcagat atagtttatc atagcggtgt cggaagaaga
     5341 attgtctcaa cgttcgttaa gtcatttcct gtggaaaatc agctaattat gatgcggtcc
     5401 ttgattgctg gccataacgt tatacgcaac tcgaataatg ttatacataa ttagatttat
     5461 atattggaat cggaaccgtt tcc
//
LOCUS       DAOSYN010000080         5439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_12614_length_5439_cov_3.674034, whole genome shotgun sequence.
ACCESSION   DAOSYN010000080 DAOSYN010000000
VERSION     DAOSYN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12614_length_5439_cov_3.674034"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PLJ11_04480"
     CDS             <1..616
                     /locus_tag="PLJ11_04480"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO03961.1"
                     /translation="CGSGLIDLVAQLFLTGALDKKGHLQPSERVREGEDGKEYVLVRS
                     RDVERDGYDLKANGMARFPMDEVERRRDITVTEADIANVIRTKAAIYAAAEVLLDTAG
                     LAFGELERVYIAGGFGHHIDLDNAVAIGLLPDLPRERFTFLGNASLDGAKLYLLSGEM
                     RKEALRVHRAMAYVELSTDQRFFERFSSATFLPHTDLSRFPSVK"
     gene            complement(617..1309)
                     /locus_tag="PLJ11_04485"
     CDS             complement(617..1309)
                     /locus_tag="PLJ11_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03962.1"
                     /translation="MRLITINRTRPNASIELYGCPMQCKYCSHTSAQFKDYDLDKVVQ
                     AMSDNGIRSVFLGGAEPAMHRKEALDLIRILHGRGKEVILKTTGADPEFLAATKGKVT
                     RYILEVKAPLDDPQLLTVVTSLPLEKAREQQENVRRSLTVLEGQKVRAVLRLIPGRYT
                     VEAVERMAEDLAGHVDELVIAQFLSSDSDVSYAGITTPSPDQATMMAFGQAARRHLPR
                     VKVKGSGFEVVL"
     gene            1447..2478
                     /locus_tag="PLJ11_04490"
     CDS             1447..2478
                     /locus_tag="PLJ11_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03963.1"
                     /translation="MAWNCAATGIGSLPHKDPQRAMDLIAASMREVPYWPQLPALGFG
                     ENMYAQFSTALPGVRLDARRNRITVDLQAYDPEDFYTAVVTDDVERFAPPVENFRGLY
                     ALLERFKGRRLGAVKGQVTGPLSAGLQVLDQNGKAAIYDEAYGEIIRRALNMQAKWQA
                     RKLREISARPIIFLDEPSLSLIGTPFASVSQRQVVEWTDEVLDGVGAIKGLHCCGNTD
                     WPLLLSTSIDLLSFDAYNYAHTIALFPKEVAHFLERGGSIAWGLIPNMEDQLAKETVG
                     TLLRRFEGYMDMLSRKGMDPALVLERSLITPQCGLGGLDEANAERALELLTGTVEAIR
                     ERYRLGDME"
     gene            2475..3227
                     /locus_tag="PLJ11_04495"
     CDS             2475..3227
                     /locus_tag="PLJ11_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO03964.1"
                     /translation="MSPLVAVAGKGGVGKSTIAALLVRVLSEGGAVLAVDADPNSNLA
                     QRLGVEVSGTVGQLREGLLSTHGQPPRDMSKQEYLRLKLREMLVEGDGFDLLTMGRPE
                     GPGCYCYVNNLLRVLVDQLMDSHRYVVIDNEAGMEHLSRRTARSMDVLLLVSDPTPVG
                     AATAGRLAELADELELKVGRKVLLMNEVREGQEERAEALAEGTGLEHYLLHEDERVRE
                     GAYAGAPLKGDEALARELREIIPRLFPSDRRR"
     gene            complement(3190..3633)
                     /locus_tag="PLJ11_04500"
     CDS             complement(3190..3633)
                     /locus_tag="PLJ11_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03965.1"
                     /translation="MRRPAALLMALLVVLSAVALPSSEAAPASPTLSAERDGARIFLS
                     WDPPSGNVTVLRYNIYRGTDPGNLTFYGSVDGNYTAGYDLEVVRGWTYYYAISANTTE
                     GEGELSPIVAVPPPGDAYPVMVMGTILVLAVGTLIFAYRKGRGEG"
     gene            complement(3795..>4564)
                     /locus_tag="PLJ11_04505"
     CDS             complement(3795..>4564)
                     /locus_tag="PLJ11_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03966.1"
                     /translation="TTEAKCKITGTISGGQEPVSKTLVFVGNKRFQATLDNDTSTFEV
                     EVPLRQGVNDIVVLATDANTFTNWAGFYRTEIESTAAMGALTVTLTWGQDNSDVDLHV
                     LEPYGRHIYYANDGYDPNGPYLDFDNTYGYGPEHYYATDDMVMYDASGVQQSTDLYGE
                     YQIAVHYYADHDDDDEEDQLITWTVTISYLVLYVEATGQEIWEEVTYTGTLGSAYSGS
                     SHSFASGGGWSEVMTFECFEPDLDDYSVPDPEDVHFE"
     assembly_gap    4565..4574
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4575..5008)
                     /locus_tag="PLJ11_04510"
     CDS             complement(<4575..5008)
                     /locus_tag="PLJ11_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03967.1"
                     /translation="MSAPSFYILSGLPGVGKSHARKTFLASIPVVDCDEIKQSLPGYD
                     PNNPGSEPSSTQQQIPFNLTSGFTQVFNSGLSAADPLQFNIFYHASAKVLSPLNAYTG
                     LYESAQDNFEDDSIFPEIELTDVDTTPEGETPIDTDADGVRDT"
     gene            complement(5005..5409)
                     /locus_tag="PLJ11_04515"
     CDS             complement(5005..5409)
                     /locus_tag="PLJ11_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03968.1"
                     /translation="MRYYVLVEKRSTSLAETSGAGAGAVVLYHGSRIRGLREILPAAI
                     TGATPTDGRAGMLDVVYVTTSIDHARSYAGRNGSVYVVDAPDARPYAEVWRDEKGRKA
                     SCARKKLAALKASDIWVASRATVVAELPGGVA"
BASE COUNT          956 a         1704 c         1802 g          967 t
ORIGIN      
        1 gtgcggctcc gggctaatcg accttgtggc gcagctgttc ctgaccggcg cattggacaa
       61 gaaggggcac ctccagccct ccgagagggt cagggagggg gaggacggga aggagtacgt
      121 cctggtccgc tccagggacg tggagaggga cggctacgac ctgaaggcga acgggatggc
      181 ccgtttcccc atggatgagg tcgagcgccg cagggacatc acggtcacgg aggcggacat
      241 cgccaacgtc atccggacca aggcggccat ctacgccgcc gccgaggtgc tgctggacac
      301 ggcgggcctc gccttcgggg agctcgagag ggtgtacatc gccggaggct tcggtcacca
      361 catcgacctc gacaacgcgg tggccatcgg cctcctgccc gatctcccca gggagcgctt
      421 caccttcctg gggaacgcct cgctggacgg ggccaagcta taccttctgt caggggagat
      481 gaggaaggag gccctgaggg tgcacagggc gatggcctac gtcgaactct cgacggacca
      541 gaggttcttc gaacgcttct cctcggccac cttcctgccg cacaccgacc tgagcaggtt
      601 tccttcagta aaataattag aggacaacct cgaagccgct gcctttgacc ttcaccctgg
      661 gcaggtgcct ccgtgccgcc tgcccgaagg ccatcatcgt ggcctggtcc ggactggggg
      721 tcgtaatgcc cgcataggac acgtcgctgt cgctggacag gaactgggcg atcactagct
      781 catccacatg accggcgagg tcctctgcca tcctctccac cgcctccacg gtgtaccggc
      841 cggggatgag gcgcaggacg gcccttacct tctgtccttc gagcaccgtc agcgacctgc
      901 gcacgttctc ctgctgctcc ctcgctttct ccagggggag cgaggtcacc acggtcagga
      961 gctgggggtc gtccagcggc gccttcacct ccaatatgta ccgggtcacc ttgcccttgg
     1021 tggctgccag gaactccggg tcggccccgg tggtcttcag gatcacctcc tttcccctgc
     1081 cgtgcaggat ccggatgagg tcgagggcct ccttgcggtg cattgccggt tccgccccac
     1141 cgaggaaaac ggagcgtatc ccgttgtcgg acatggcctg gacgaccttg tccaggtcat
     1201 agtccttgaa ctgggcggac gtgtgcgagc agtacttgca ctgcatcggg cagccgtaga
     1261 gttcgattga ggcgttcggc ctagtcctgt tgatggtgat cagccgcatc gtccccctat
     1321 tcggcgaaag gcccataatg ctttcacatg gcccttggac ggcctaggct gtagtggcgt
     1381 ccggaccacc gcccatctcc aggccgcgac cgaattgttt tataacggga aggacaactc
     1441 cggagcatgg cctggaactg cgccgccacc gggatcggct ccctcccgca caaggacccc
     1501 cagcgggcga tggacctcat cgccgccagc atgcgtgagg tcccctattg gccccagctg
     1561 ccggccctgg gcttcgggga gaacatgtac gcgcagttct caaccgccct gccgggggtg
     1621 cggctcgatg cgcggaggaa ccgcatcaca gtggacctcc aggcctacga ccccgaggat
     1681 ttctacaccg cggtggtcac cgacgacgtg gagcgcttcg ccccccctgt cgagaacttc
     1741 agggggctgt acgccctgct cgagcggttc aaggggcgga ggctgggggc ggtcaagggg
     1801 caggtcaccg gcccgctcag cgcagggctc caggtcctgg accagaacgg taaggccgcg
     1861 atctacgacg aagcgtacgg ggagatcatc cggcgggcgc tcaacatgca ggccaagtgg
     1921 caggcccgga agctcaggga gatctcagcc cgccccatca tcttcctgga cgagccctcg
     1981 ttgagcctca tcggcacccc gttcgcctcc gtctcccaaa ggcaggtggt ggagtggacc
     2041 gacgaggtgc tggacggcgt gggcgccatc aaggggctgc actgctgcgg caacaccgac
     2101 tggccgttgc tgctgtcgac gtccatcgac ctgctgagct tcgacgccta caactacgcg
     2161 cataccatcg ccctgttccc caaggaggtc gcccatttcc tcgagcgggg cgggtccata
     2221 gcctgggggc tcatccccaa catggaggac cagctcgcca aggagacggt ggggacgctg
     2281 ctgaggaggt tcgaggggta catggacatg ctctcccgca agggaatgga cccggccctg
     2341 gtcctggagc gctcgctcat caccccacag tgcgggctcg gcgggctgga cgaggcgaac
     2401 gcggagagag cgctggaact gctgaccggc accgtcgagg ccattcggga gcgctaccgg
     2461 ctgggggaca tggaatgagc cccctggtgg ccgtggccgg caaggggggc gtgggcaaga
     2521 gcaccatcgc cgccctgctc gtgagggtcc tttccgaagg gggcgcggtc cttgcggtcg
     2581 acgcggaccc caacagcaac ctcgcgcaga ggctgggggt cgaggtctca gggacggtgg
     2641 ggcagctccg ggaggggctg ctctccacgc atgggcagcc tcccagggac atgagcaagc
     2701 aggagtacct gaggctcaag ctccgggaga tgctggtgga gggcgacggc ttcgacctgc
     2761 tcacgatggg ccggcccgag ggccccggct gctattgcta cgtgaacaac ctcctccgcg
     2821 tgctcgtgga ccagctcatg gactcccatc gctatgtcgt aatcgacaac gaggcgggga
     2881 tggagcacct ctccaggcgc accgccagga gcatggacgt attgctgctg gtctcggacc
     2941 cgaccccggt gggggcggcc accgccggac gcctggcgga gctcgcggat gagctggaac
     3001 tgaaggtcgg gagaaaggtc ctgctcatga acgaagtgcg cgagggccag gaggaaaggg
     3061 cggaggcgct ggccgagggc accggcctgg agcactacct tctccacgag gacgagaggg
     3121 tgagggaagg ggcatacgcc ggggcgccgc tcaaagggga cgaggccctg gcaagggagc
     3181 tgagggagat catccctcgc ctcttccctt ccgataggcg aagatgaggg tgccgacagc
     3241 caggaccaat atcgtcccca taaccatcac gggatacgcg tcgcccgggg gagggacggc
     3301 cacgatcggg ctgagctccc cctccccctc ggtggtgttg gcgcttatgg cgtagtagta
     3361 cgtccatccg cgcacgacct cgagatcgta cccggcggtg tagttgccgt ccaccgaacc
     3421 gtagaacgtc aggttccccg ggtccgtccc gcgatagata ttgtaacgga ggaccgtcac
     3481 gttcccggac ggggggtccc aggagaggaa tatcctggcg ccgtcgcgtt cggcggagag
     3541 ggtcggcgag gccggggccg cctcgctcga cgggagcgcg acggcgctca agaccacgag
     3601 caaggccatc agaagggctg cggggcgcct cacggccatc catcgccccc tgcgatataa
     3661 atggtttcca ggtccgtggc cacggcgcgg tcggtgggat ggcccacgac aaacggtaga
     3721 gaagtgaaga aagaaggtcg tcgaagggcc ccggggacgg ggcccttcaa cagaaaaatg
     3781 gtttgggggg cgcctcattc gaagtgcacg tcctccggat ccggaacgct atagtcgtcc
     3841 aggtcgggct cgaagcactc gaacgtcatt acctccgacc atccgccccc ggaggcgaac
     3901 gagtgggagc tgccggagta ggcgctgccg agggtgcccg tgtacgtgac ctcctcccag
     3961 atctcctggc ccgtggcctc gacgtagagc accaggtagg aaatggtgac ggtccaggtg
     4021 atgagctggt cctcttcatc gtcgtcatca tggtccgcgt agtagtggac ggcgatctgg
     4081 tactcgccat acaggtcggt gctctgctgc acgccggagg cgtcgtacat caccatgtca
     4141 tcggtggcgt agtaatgctc cggaccgtac ccataggtgt tgtcgaagtc caggtatggg
     4201 ccgttcgggt catagccatc attggcatag tatatatggc gcccgtaggg ttcgaggacg
     4261 tgcaggtcga cgtcgctgtt gtcctgcccc caggtgaggg tgaccgtcag cgctcccatg
     4321 gcggcggtgc tctctatctc cgtcctatag aagccggccc agttggtgaa ggtgttggcg
     4381 tcggtcgcaa gcaccacgat gtcgttcact ccctgcctca gcggcacctc gacctcgaag
     4441 gtggaggtgt cattatccag ggtggcctgg aacctcttgt tcccgacgaa caccagggtc
     4501 ttggacacgg gctcctggcc gccggagatg gtgcctgtga tcttacactt ggcctcggtg
     4561 gtgtnnnnnn nnnngtgtcg cggacgccgt cggcatcggt gtcaatagga gtctctccct
     4621 caggcgttgt gtccacatcc gtcagctcga tctccgggaa tatcgaatcg tcttcgaagt
     4681 tgtcctgggc gctctcatac agcccggtgt aggcgttcag cgggctgagc accttggcgc
     4741 tagcgtgata gaagatgttg aactgcagtg ggtcggcggc gctaaggccg gagttgaaca
     4801 cctgggtgaa gcctgatgtc agattgaagg ggatctgttg ctgtgtgcta ctcggctcgc
     4861 tccccgggtt gttcgggtca taccccggca aggactgctt tatctcgtcg cagtccacga
     4921 ccggaatgga ggccaagaag gtcttccggg catgggactt gcccacgccg ggaagcccgg
     4981 acaagatata gaaggagggc gcgctcatgc cacacccccc gggagttcgg ccacgacggt
     5041 cgcccttgag gcgacccaga tatcgctcgc cttgagcgcg gcgagctttt tccgggcgca
     5101 agaggccttg cgcccttttt cgtctcgcca gacctcggca tatggtctgg cgtcgggagc
     5161 gtctactaca tagacactcc catttcgccc ggcataggac cgggcgtggt cgatggacgt
     5221 ggtcacgtag accacgtcca acatcccggc gcggccatcg gtcggcgtcg cgccggtgat
     5281 ggctgcgggc aagatctccc gcagcccacg gatccgggat ccgtggtaca gcaccacggc
     5341 cccggctccg gctccgcttg tctctgctaa acttgtggat ctcttttcca cgagcacata
     5401 atacctcacc acctcctaac ctgtcaaccc caaatcgca
//
LOCUS       DAOSYN010000081         5375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_12792_length_5375_cov_3.320489, whole genome shotgun sequence.
ACCESSION   DAOSYN010000081 DAOSYN010000000
VERSION     DAOSYN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12792_length_5375_cov_3.320489"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..2545
                     /gene="acnA"
                     /locus_tag="PLJ11_04520"
     CDS             2..2545
                     /gene="acnA"
                     /locus_tag="PLJ11_04520"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOO03969.1"
                     /translation="MSYYSLKKLGASTDRLPFSIKVLLESLLRQEDGVLVTREDVEHL
                     LAWTPAGNAEVDIPFLPARVVLQDFTGVPCVVDLAAMRDAVRGRGGNPSRINPLVPVD
                     LIIDHSVQTDYHGTCYARELNERKEMERNLERYSLLKWAQGSFNNLRVVPPANGIIHQ
                     VNLECLASVVASRDGVAFPDSVFGTDSHTTQVNALGVLGWGVGGIEAEAVMLGQPCYM
                     PLPQVVGFRLTGKLGPGVTATDMVLTAVEMLRRKGVVGKFVEFYGPGIKGLSLPDRAT
                     LANMAPEYGATMGFFPVDEVTIAYLKQTGRERLCELVERYCREQGLFYEGQSPAYSDH
                     LELDLRTVRPCLAGPKRPQDRIELARMKQGFQEMMARQGGQRQGRLLRDGDVLIASIT
                     SCTNTSNPSVMMAAGLLAKRAVERGLRPHPNVKTSLAPGSKAVTAYLEKAGLLPYLEA
                     LGFHVVGYGCMTCIGNSGPIRQELAEDIEANRLTVAAVLSGNRNFEGRISPHAKANYL
                     ASPPLVVAYALAGTVDLDLEHEPLGVDPNGMPVFLRDLWPTAKEVKAALRKVTPAMYR
                     KAYSTVFQGSELWDGVPAPRGETFAWDPASTYIRKVPFFDGMAEEAGGPAPIRGARAL
                     AVLGDSITTDHISPAGEIPADSVAGQYLISLGVGEDAFNSYGARRGNHEVMVRGTFAN
                     PRLRNELAGGREGGYTTLQPDGTPMDIHSACEEYRRRGVPLIILAGKEYGTGSSRDWA
                     AKGPYLLGVRAVIAESFERIHRSNLVGMGIIPLQFEPGQGVRSLGLDGTEEFDVELEF
                     KPRALAKVTARKGGKGIEFNAIVRIDTPVEVEYLRNGGILQTVLRKLTA"
     gene            2581..3561
                     /locus_tag="PLJ11_04525"
     CDS             2581..3561
                     /locus_tag="PLJ11_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO03970.1"
                     /translation="MSDGEAVIEAEGLTKVYPAAFRLFGDGGREKLAVDNVTFNVRRG
                     EIFGLLGPNGAGKTTTVKMLSTLLIPTSGTATVLGLDVVRDAGKLRSRINLVSGGERG
                     LYFRLTGRQNLEFFSDMYHVPREEKRRRITNLLEIVGLTEAADVRVEEYSRGMKQRLH
                     LARGLVNEPEVLFLDEPTLGLDPEISRETRALIRHLSEEGVTILLTTHYMFEADELCD
                     RLAIISEGRIKALGSPYSMKEAVRNDSIIEVEARELGEAQRRELADLPGVSAVSVEHL
                     EGRQRARIVVANAPALIPTVAAMLESRRVISIRVDQPTLEDAYIKLVNGR"
     gene            3561..4343
                     /locus_tag="PLJ11_04530"
     CDS             3561..4343
                     /locus_tag="PLJ11_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO03971.1"
                     /translation="MSVRAMKAALKQQAIHFFVDPQWVIPSIIAPFIFTTVTLFLFRH
                     TGGDLMLYAVLGGGVLGMWGNTIHSSSWSIGYDRMNGTLEAIMVTPAPLSSVVFGRCL
                     WNAFLGLLNALAVFLVAQFAFGIDVTVRDPLGFLVALAATLLSLAVLGMVFGAFYVVT
                     RASSALFQMLEYPIYVLSGAMFPLVVLPEWSRPFSYALPATWGAEALKIASLPGYDPL
                     GLGYMGALGLTALTTLVFLLVSLWLFSFLEGRAKVNGTLVRY"
     gene            4345..5139
                     /locus_tag="PLJ11_04535"
     CDS             4345..5139
                     /locus_tag="PLJ11_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO03972.1"
                     /translation="MMRNVTGVKASALVAMRSQFAVVGPVLWLIQILSTALFQMWFFV
                     LVSDFAGDPAAGPAYVALGNAVSSLTYAAVYGVTMAAGSEKHIGTMASILGTPTRMYY
                     VFLGKGAYQSVIGLFTVTVSLLLSSWLFGVDLSGADPLTLATVLVVTCFAMVGFGMVI
                     GSVGVYLRSSMVLGSIVLYLGLLLCGVNFPIAYLPGWLQPASYAFPLTYGVEAVRRVA
                     MGAGLADVSHLLIVMLAMGAAFYVLAYLLFEVFERLALRRGSLDMF"
BASE COUNT          840 a         1744 c         1876 g          915 t
ORIGIN      
        1 cctgagctat tattccttga agaagctggg ggcttcgacc gaccgccttc cgttctccat
       61 aaaggtgctc ctggagtccc tcctccgcca ggaggacggg gtgctggtga ccagggagga
      121 tgtggaacat ctgctcgcct ggacccctgc cgggaacgcc gaggtggaca tccccttcct
      181 tcctgcccgg gtggtcctgc aggacttcac gggcgtgcca tgcgtcgtgg acctggccgc
      241 catgcgcgac gccgtcaggg gaaggggcgg gaacccctcg cgcatcaatc ccctggtccc
      301 ggtggacctg atcatcgatc actcggtcca gaccgattac cacgggacgt gctatgcgcg
      361 ggagctcaac gagcggaagg agatggagcg gaacctggag cggtattcgc tcctcaagtg
      421 ggcccagggc tccttcaaca acctgagggt ggtcccgcca gcgaacggca tcatccacca
      481 ggtgaacctg gagtgcctgg cgtccgtggt cgcgagccgg gacggcgtgg cgttccccga
      541 ttcggtcttc gggaccgatt cccacactac ccaggtgaac gccctcggcg tgctagggtg
      601 gggcgtgggg ggcatagagg cggaggcggt catgctcggc cagccctgtt acatgccatt
      661 gccgcaggtg gtgggcttca ggctcaccgg gaagctgggg ccgggggtca ccgccaccga
      721 catggtgctg accgcggtgg agatgctgcg gaggaagggc gtggtgggca agttcgtgga
      781 gttctacgga cctggcatca aggggctgtc gctcccggac cgcgcgacgc tggcgaacat
      841 ggccccggag tacggcgcga ccatgggctt cttcccggtg gacgaggtga ccatcgccta
      901 tctcaagcag accgggcggg agcgcctctg cgaactcgtc gagcggtact gcagggagca
      961 ggggctcttc tacgaggggc agagccccgc gtatagtgac catctcgagc tggacctcag
     1021 gacggtgagg ccgtgcctgg ccgggccgaa gcgcccccag gaccgcatcg agctggcccg
     1081 gatgaagcag gggttccagg agatgatggc aaggcaagga gggcagcgcc aaggcaggct
     1141 gctgagggac ggggacgtcc tcatcgcctc gataacttcg tgcaccaaca cctccaaccc
     1201 gtccgtgatg atggccgccg ggctcctcgc caagagggcg gtggagaggg gattgcgccc
     1261 ccacccgaac gtgaagacgt ccctggcccc ggggagcaag gcggtgacgg cctatcttga
     1321 gaaggccggc ctccttccct acctggaggc cctggggttc cacgtcgtgg ggtacgggtg
     1381 catgacgtgc atcggcaact ccggcccgat ccggcaggaa ctggccgagg acatcgaggc
     1441 caaccgcctc acggtggcgg cggtgctctc gggcaaccgc aacttcgagg gccgcatctc
     1501 gccgcacgcc aaggcgaact acctggcctc cccgccgctg gtggtggcct acgccctcgc
     1561 cggaacggtc gacctggacc tggagcatga gccgctgggc gtggacccga acggcatgcc
     1621 ggtgttcctg agggacctct ggccgacggc gaaggaggtc aaggcggcgc tgcggaaggt
     1681 caccccggcc atgtaccgga aggcctactc gaccgtcttc caggggagcg agctgtggga
     1741 cggcgtcccc gcccccaggg gggagacgtt cgcctgggac cccgcctcca cgtacatccg
     1801 caaggtcccc ttcttcgacg gcatggccga ggaggcgggg gggccggcgc cgatccgagg
     1861 ggcccgggcc ttggcggtgc tgggggacag catcacgacc gatcacatct ccccggcggg
     1921 ggagatcccg gcggacagcg tcgccggcca gtacctcatc tccctggggg ttggggagga
     1981 tgccttcaac tcctatgggg cgcgccgcgg caaccatgag gtcatggtgc gcgggacgtt
     2041 cgccaatccc aggctgagga acgagctggc gggcggaagg gagggcggat acacgacgct
     2101 gcagccggat gggacgccga tggacatcca ctccgcctgc gaggagtata ggcggagggg
     2161 cgtgcccctc atcatactgg ccgggaagga gtacgggacg gggagctccc gggactgggc
     2221 ggcgaagggg ccgtacctcc tcggcgtcag ggctgtcatc gccgagtcct ttgagcgcat
     2281 ccaccgcagc aacctggtgg gcatggggat catcccgctc cagttcgagc ccgggcaggg
     2341 cgtccgctcg ctcggcctcg atggcaccga ggagttcgac gtggagctcg agttcaagcc
     2401 cagggcgctc gccaaggtga ccgcgcggaa gggagggaag ggcattgagt tcaacgcgat
     2461 cgtccgtata gataccccgg tggaggtgga atacctccgc aacggcggca tcctccagac
     2521 cgtgctgagg aagctgacgg cgtaacctca ttataatcgc gagggcgttg cgccaccgtc
     2581 atgagcgacg gggaggcggt catagaggcc gaggggctca ccaaggtgta tcctgcggcc
     2641 ttcaggctct tcggggacgg gggaagggag aagctggccg tggacaacgt caccttcaac
     2701 gttcgccggg gggagatctt cgggctcctg ggcccgaacg gcgcggggaa gacgaccacg
     2761 gtcaagatgc tctcgaccct gctcatcccc acctccggca cggccaccgt cctggggctg
     2821 gacgtggtca gggacgccgg gaagctcagg tcgaggatca acctggtctc ggggggcgag
     2881 cgcgggctgt acttccggct caccggccgc cagaaccttg agttcttctc ggacatgtac
     2941 cacgtcccgc gggaggagaa gcggaggagg atcaccaacc tgctcgagat cgtcgggctc
     3001 accgaggcgg cggacgtccg ggtggaggag tactcccggg gcatgaagca gcgcctgcac
     3061 ctcgcccgcg ggctggtgaa cgaaccggag gtcctattcc tggatgagcc cacgctcggc
     3121 ctcgacccgg agatatccag ggagaccagg gcgctgatcc gccacctgtc cgaggagggc
     3181 gttaccatcc tgctcaccac ccattacatg ttcgaggcag acgagctctg cgatcgcctt
     3241 gccatcatct ccgaggggcg catcaaggcc ctcggctccc cgtactccat gaaggaggcg
     3301 gtgcgcaacg actcgatcat cgaggtcgag gcccgggagc tcggggaggc ccagcggcgg
     3361 gagctggccg atctgcccgg cgtgtccgcg gtctcggtgg agcacctcga ggggcggcag
     3421 cgggccagga tcgtcgtggc caacgccccc gcgctcatcc caaccgtcgc ggccatgctg
     3481 gagagccgca gggtgatcag catccgggtg gaccagccca cgctcgagga cgcctacatc
     3541 aagctggtga acgggagatg atgagcgtcc gggccatgaa ggccgccctc aagcagcagg
     3601 ccatccattt cttcgtagac ccccagtggg tgatcccgag catcatcgcc cccttcatct
     3661 tcaccacggt caccttattc ctgttcaggc acaccggggg ggacctgatg ctctacgccg
     3721 tgctgggggg aggggtgctg ggcatgtggg ggaacaccat ccacagcagc agctggtcca
     3781 tcgggtacga ccggatgaac ggcacgctgg aggcgatcat ggtcaccccc gcccccctgt
     3841 cgtccgtggt gttcggccga tgcctttgga acgccttcct cgggctgctg aacgcgctgg
     3901 ccgtcttcct cgtggcccag ttcgccttcg gcatcgacgt caccgtgcgg gaccccctcg
     3961 gcttcctggt ggccctggcg gcgacgctcc tctccctggc cgtgctaggc atggtcttcg
     4021 gggccttcta cgtggtcacg cgggcctcct ccgccctctt ccagatgctc gagtacccga
     4081 tctacgtcct ctcgggggcg atgttcccgc tcgtggtcct gccggaatgg tccaggccgt
     4141 tctcgtacgc cctcccggcc acatgggggg cggaggcgct gaagatcgcc tccctgcccg
     4201 ggtacgaccc gctcggcctg ggatacatgg gcgcgctggg gctgacggcg ctcacgaccc
     4261 tcgtcttcct tctggtctcc ctctggctct tctccttcct ggaggggagg gccaaggtga
     4321 acggcacgct ggtgaggtac tgacatgatg cggaacgtga cgggggtcaa ggcatcggcc
     4381 ctggtggcca tgcgctcgca gttcgcggtg gtcggcccgg tgctgtggct catccagatc
     4441 ctttccaccg ccctcttcca gatgtggttc ttcgtgctag tgagcgattt cgccggcgac
     4501 cccgcggccg ggccggccta cgtggccttg ggcaacgcgg tgtcctcgct cacctatgca
     4561 gcggtgtacg gggtgacgat ggcggcgggg tcggagaagc acatcgggac catggccagc
     4621 atcctgggca ccccgacccg catgtactat gtcttcctgg ggaagggggc gtatcaatcc
     4681 gtaatagggc tgttcaccgt cacggtctcc ctgctcctct cctcctggct cttcggagtg
     4741 gacctgtcgg gcgcggaccc cctgaccctc gcgacggtcc tggtggtcac ctgcttcgcc
     4801 atggtagggt tcggcatggt catcggcagc gtcggcgtct acctccgctc gtccatggtc
     4861 ctcggctcca tcgtgctcta cctgggcctg ctcctttgcg gggtgaactt cccgatagcg
     4921 tacctgccgg ggtggctcca gccggcctcc tacgccttcc cgctcaccta cggggtggag
     4981 gcggtgcggc gtgtggccat gggcgccggg ctggcggacg tgtcccatct cctcatcgtc
     5041 atgcttgcca tgggggccgc gttctacgtc ctggcatacc tgctcttcga ggtgttcgaa
     5101 cgacttgccc tgaggcgagg ctccctggac atgttctgag cctggaggcc cgcgcccgct
     5161 tagggatcgg ggaacgccag agcacatcgg ccctgcccgg ggctgggacc gagtagccgg
     5221 tccctcagcg ggagccgcgg cctcgccgcg tcatctcggc gttgggaccg gcccttcctt
     5281 gcgcgccagg ttgacgtccc cgacggggat ccaatcgtcc agggtatgga agtcgcggcg
     5341 ggtgaagcgg aagggcggaa cctcctcgaa ccggt
//
LOCUS       DAOSYN010000082         5324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_12936_length_5324_cov_3.808503, whole genome shotgun sequence.
ACCESSION   DAOSYN010000082 DAOSYN010000000
VERSION     DAOSYN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12936_length_5324_cov_3.808503"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLJ11_04540"
     CDS             <1..591
                     /locus_tag="PLJ11_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOO03973.1"
                     /translation="PKDRKGYSGVAMYTKVEPRRVVPGLGRERFDSEGRVLVAHFDAF
                     VLFNIYFPNGKASPERLRYKMEFYEEVLEVAKATVDGGRHVVLCGDVNTAHREIDLAR
                     PRENERTSGFLPQERQWIDRFLAAGFVDTFRLFDPSPERYTWWDMKTRARERNVGWRI
                     DYFFVDEGLRARCRSAFIMPEVTGSDHCPIGLELEV"
     gene            674..1063
                     /locus_tag="PLJ11_04545"
     CDS             674..1063
                     /locus_tag="PLJ11_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO03974.1"
                     /translation="MSVVLATDGRPHTAKAVDYALEYARLHHEPLYVVFVISPRHEEN
                     REGIVGEAKRHLEEIKGRAAQQGVAVTALLETGPPAESIIAVAERVGASAIIVGTSGK
                     SVLDRMLIGSVSEHVVRTSPCSVIVVK"
     gene            complement(1056..1808)
                     /gene="nuoB"
                     /locus_tag="PLJ11_04550"
     CDS             complement(1056..1808)
                     /gene="nuoB"
                     /locus_tag="PLJ11_04550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOO03975.1"
                     /translation="MLDIIERRLREKRTATEPLAELQQGGRTRAPMADARCDLCGACA
                     RQCPTSAITATEDWSVDVGRCIFCGDCQPACPKGAITLSREVPAVSDRSSLILRRALP
                     LPELRFHLRDEVQRVLGRSLNIREVDAGSCNGCEVEVNSLSNPVYDLERFGIRIVASP
                     RHADALLVTGPVTRNMRPALMRTYRAVPEPRIVVAMGTCAISGCPFGGTYAAGAGVAE
                     VLPVDIFIPGCPPHPRTVVLALLEALGRTQVT"
     gene            complement(1817..3268)
                     /locus_tag="PLJ11_04555"
     CDS             complement(1817..3268)
                     /locus_tag="PLJ11_04555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOO03976.1"
                     /translation="MKVPEDLLRPLLLHLLRREEGELANLYYVHDGNRMVAVVLIPRH
                     GTVVVEAGLEGEGYQSLTPEVPAANWYEREMLEMNGIVPLDHPDPRPLRLHEWPRGLR
                     PLDPGFPIDAEVPLTGEPYRYRRVEGDGVLEVPVGPVHAGVIEPGHFRFSTAGEPILN
                     LEVRLGYVHRGVEKALEGCPLGRALRLVERISGDNGVAHSLAFCQVVEMGAPVPERAQ
                     LLRTVFTELERIHCHLGDIAGIATDVAFAVPAAEASVLRERMLRLNERLTGHRLLWGT
                     MAVGGVAKDLDAEACAFLERALVELGLAFEPLVDSLKGSPSFLDRMETTGVLPLRTAK
                     ELRATGPVARASGWDRDTRRDFPYAAYPRLGFLVPVRREGDVLARLMVRIEEVRESIS
                     MVKQCLDHLGEGELRIEVPAPEGFGLGLVEAPRGELMHCAHFRSGAIERYKVRDPSFC
                     NWPTMERAVLGNIVPDFPVINKSYGLSYSGNDL"
     gene            complement(3346..4803)
                     /locus_tag="PLJ11_04560"
     CDS             complement(3346..4803)
                     /locus_tag="PLJ11_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HOO03977.1"
                     /translation="MIEALLLVPLFTALLCGLARSERTIYVLSAAGSTVTALVAFPCC
                     YQAFSGPPLHRGPWYLDPLSALFVLVIVVITLLVSVYSVQYLQHDRREGEVNVKDLRY
                     YHVLLHLFALCMLLVVVVDSLGLMWIAVEGTTLISAFLVGFYKKGEAIEASWKYLIIC
                     SVGITLALLGIVLLYASSMAALGEGADTLDWSALRAVADQLDPALVKVAFIFIVVGLG
                     TKAGLAPMHTWLPDAHSQSPTPISALLSAVLLNCAMYGVLRVHILAQAAVPGFSSSLL
                     TFFGLLSVAIATAFLLTSRDLKRFLAFSSVEHMGIIALGFGIGGPLAVLGALFHVVAH
                     SLTKTLMFFGAGNVIQRYRTREMREIKGVGRTMPATASLLVLGGAAIAGSPPFAIFIG
                     ELLITAGALASGEYLVAGLFIALMAIIFAAMTYRVLGMVSGRPQEGVPAGELGTLRLM
                     PMLVLAAAIIVLGVLLPSALGDALGSVAELFEVII"
     gene            complement(4805..>5324)
                     /locus_tag="PLJ11_04565"
     CDS             complement(4805..>5324)
                     /locus_tag="PLJ11_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOO03978.1"
                     /translation="AAVLAHYTGSEHIYVVAGLTLVVKGVVIPRFLIYSMDRLKVRRE
                     IEPLVGIPASLLISGGLVIVAYYITEPLISSAETVTRNCLAVSLAVVLIGLFMMISRR
                     KAMTQMVGLLMMENGLFLGIISTTYGMPLIVEIGIFFDVLMAVLIMGIFAYRINQTFE
                     TLDTTFMRRLRG"
BASE COUNT          961 a         1738 c         1834 g          791 t
ORIGIN      
        1 cccaaggacc ggaagggtta cagcggggtg gccatgtaca cgaaggtcga accgagaagg
       61 gtcgtccctg gccttggccg ggagaggttc gattccgaag ggcgcgtcct cgtggcccat
      121 ttcgacgcct tcgtgctctt caacatctac ttccccaacg ggaaggcctc cccggaacgg
      181 ctgcggtaca agatggagtt ctacgaggag gtcctggagg tggccaaggc caccgtggac
      241 ggcgggaggc acgtcgtcct ctgcggggac gtcaacaccg cgcacaggga gatcgacctg
      301 gcccggccga gggagaatga gaggacctcc ggcttcctcc ctcaggagcg ccaatggata
      361 gaccggttcc tggcagcggg ctttgtggac accttccgcc tcttcgaccc gtccccggag
      421 cgctacacct ggtgggacat gaagacccgc gcccgggagc ggaacgtcgg gtggcgcatc
      481 gactacttct ttgtggacga gggattgcgg gcacggtgcc ggagcgcctt cataatgccc
      541 gaggtgacgg gctccgacca ttgcccgata gggctggagc tggaggtgtg acctccggcg
      601 cccggccgcg ccgcaggacg gcattgacaa ttttatatcc tggcgaagcc aatcccgatg
      661 tggaagtggc gcgatgagcg ttgtattggc taccgacggt agaccgcata cggccaaggc
      721 ggtcgattac gcattggagt acgcgaggct gcaccatgag ccgctgtacg tcgtctttgt
      781 catcagccct cggcacgagg agaaccggga ggggatcgtg ggcgaagcca agaggcatct
      841 ggaggagatc aagggaaggg cggcccagca gggcgtggcg gtcaccgccc tccttgagac
      901 cggccccccg gcggagtcca tcatcgcggt ggccgagcgg gtgggcgcct ccgcgatcat
      961 cgtcggcacc tcgggcaagt ccgtcctgga ccggatgctg ataggcagcg tctccgagca
     1021 cgtcgtgcgc acctcaccct gctcggtcat cgtggtcaag tgacctgggt gcgcccgagg
     1081 gcctcaagga gcgccaggac cacggtgcgg gggtggggag ggcagccggg gatgaagatg
     1141 tccaccggca gcacctccgc caccccggcc ccggcggcgt aggtgcctcc gaacgggcag
     1201 ccggagatgg cgcaggtgcc catggccacc acgatgcggg gctccgggac ggcccggtac
     1261 gtcctcatca gggctgggcg catgttcctg gtcaccggcc cggtgacgag gagggcgtcg
     1321 gcgtgccggg gcgacgccac gatcctgatg ccgaagcgct ccagatcgta tacgggattg
     1381 gacagcgagt tcacctcgac ctcgcatccg ttgcaggagc ccgcgtccac ctcccttatg
     1441 ttcagggacc tcccgagcac cctttggacc tcgtcccgga gatggaacct cagctcaggc
     1501 agcggaaggg cgcgacgcaa tatcagcgag gagcggtcgg agacggccgg gacctcgcgg
     1561 gagagcgtga tcgccccctt cgggcaggcg ggctggcagt ccccgcagaa gatgcatctg
     1621 ccgacgtcta cgctccagtc ctccgtggcg gtgatggcgg aggtcgggca ttggcgggcg
     1681 catgccccgc acaggtcgca ccgggcgtcg gccatggggg cgcgggtccg ccctccctgt
     1741 tggagttccg ccagcggctc cgtggccgtc cgcttctccc tgagcctcct ctcgatgatg
     1801 tccagcatat cctcacctac agatcgttcc ctgaatagga caggccatag ctcttattta
     1861 tcaccgggaa gtccgggacg atgttgccga gcaccgctct ctccatcgtt ggccaattgc
     1921 agaaggaggg gtcccggacc ttgtacctct cgatcgctcc ggaacggaag tgcgcgcagt
     1981 gcatcagctc cccccgcggc gcctccacca ggccgaggcc gaacccctcc ggggccggga
     2041 cctcgatgcg cagctcccct tccccgagat ggtccaggca ctgcttgacc atgctgatcg
     2101 actcccgcac ctcctcgatc cgcaccatga gcctggccag gacgtccccc tccctacgca
     2161 ccgggaccag gaaccccagg cgggggtagg cggcatacgg gaagtcccgg cgggtgtcgc
     2221 ggtcccaccc cgatgccctg gccaccggtc ccgtggctcg cagctccttg gccgtccgca
     2281 gcgggagcac ccccgtggtc tccatccggt ccaggaagga gggggagccc ttgagcgagt
     2341 ccacgagggg ctcgaaggcc aaccccagct ccacaagggc gcgctcgagg aacgcacatg
     2401 cctcggcgtc caggtccttt gcgaccccgc ccacggccat ggtcccccac agcaggcggt
     2461 gcccggtcag cctctcgttg aggcgcagca tgcgctcccg caggacggag gcctcggcgg
     2521 ccgggaccgc aaaggccacg tcggtggcga tgcccgcgat atcgcccaga tggcagtgga
     2581 tccgctccag ctcggtgaag accgtgcgca gcagctgggc cctctcaggg accggggccc
     2641 ccatctccac gacctggcag aaggccaggg aatgggcgac gccgttgtcc cccgagatcc
     2701 gctcaaccag gcgcagcgcc cggccgagcg ggcagccctc gagcgccttc tccacgccgc
     2761 gatgcacgta gcccagcctt acctccaggt tcaggatggg ctcgccggcg gtgctgaacc
     2821 ggaagtgccc cggctcaatg accccggcat gaacggggcc gaccgggacc tccaggacgc
     2881 cgtccccctc caccctgcga tatcggtagg gctctccggt gagagggacc tcggcgtcga
     2941 tcgggaaccc ggggtcgaga gggcgcaggc ccctcggcca ttcatgcagc cggagcggcc
     3001 ttgggtccgg atggtccaac gggacgatcc cgttcatctc cagcatctcc cgctcgtacc
     3061 agttggccgc cgggacctcg ggggtgaggg actgatagcc ctcgccctcg agcccggcct
     3121 ccaccacgac ggtcccatgg cgggggatga gcaccacggc caccatcctg ttcccgtcgt
     3181 gcacatagta gaggttcgcc agctcgccct cctcccggcg gagcaggtgg aggaggaggg
     3241 gacgaagcag gtcctcgggg accttcaagt gcaaccgtcc ggagcgctcc tcgaacgaga
     3301 gcggagggaa gggaagggag tccagcagct cgacgttgta gtatctcaga tgatcacctc
     3361 gaacagttcg gcgaccgagc ccagggcgtc acccagggct gaggggagga gcacccccag
     3421 cacgatgatg gcggccgcaa ggacgagcat cggcatcagg cggagcgtgc cgagctcccc
     3481 ggccggaacg ccctcctgcg gccttccgga caccatcccc aggacgcgat aggtcatggc
     3541 ggcgaagatg atggccatga gggcgatgaa gaggccggcg acgaggtact caccgctggc
     3601 cagcgccccg gcggtgatga gcagctcgcc gatgaatatg gcgaagggcg gcgaccctgc
     3661 gatggccgcc ccgccgagga ccagcaggga ggcggtggcc ggcatggtcc tgcccacccc
     3721 cttgatctcc cgcatctccc tggtgcggta cctctgtatg acgttgccgg ccccaaagaa
     3781 catcagggtc ttggtcaggg aatgggcgac gacgtggaag agggccccga gcacggccag
     3841 cggccctccg atcccgaagc ccagggcgat gatccccatg tgctccacgc tggagaacgc
     3901 caggaaccgc ttgaggtccc tggaggtgag gaggaaggcc gtcgctatgg ccactgacag
     3961 cagcccgaag aacgtgagca gggaggagga gaagccgggc accgccgcct gggccaggat
     4021 gtgcaccctc agcaccccgt acatggcgca gttgagcagg acggcggaga ggagggcgct
     4081 tatgggggtg ggggattggc tgtgggcgtc gggcagccag gtatgcatcg gcgccaggcc
     4141 ggccttcgtc cccaggccca ccacgatgaa gatgaaggcc accttcacca gggcggggtc
     4201 cagctggtcg gcgacggcgc gcagcgcgga ccagtccagg gtgtctgcgc cctcccccag
     4261 cgcggccatc gaggaggcgt agagcaggac gatcccgagg agggcgaggg tgatgcccac
     4321 cgagcagatg atcaggtact tccaggaggc ctcgatggcc tcccctttct tgtagaaccc
     4381 caccaggaag gcggagatca gggtggtgcc ctccacggct atccacatca gcccgaggga
     4441 gtcgaccacc acgaccagga gcatgcagag ggcgaagagg tggaggagga cgtggtagta
     4501 gcgcaggtcc ttgacgttga cctcgccctc cctccggtca tgctgcaggt actgcaccga
     4561 gtagacgctc acaaggagcg tgatgaccac gatcacgagc acgaagagcg cggacagggg
     4621 gtccaagtac catgggcccc ggtgcagcgg tgggccggag aaggcctggt agcagcaggg
     4681 gaacgctacc agggcagtga ccgtggagcc ggccgcgctg aggacgtaga tggtccgctc
     4741 gctgcgcgca aggccgcaga gcagggcggt gaaaaggggg accagcagga gcgcttcgat
     4801 catactatcc cctcagcctc ctcatgaagg tcgtgtccag ggtctcgaac gtctggttga
     4861 tgcggtaggc gaatatgccc atgatgagca cggccatgag cacgtcgaag aatatcccga
     4921 tctccacgat gagcggcatg ccgtacgtgg tggagattat gcccaggaac agtccgttct
     4981 ccatcatcag caggccgacc atctgggtca tggccttgcg ccggctgatc atcatgaaga
     5041 ggccgatgag cacgaccgcc agcgagaccg ccaggcagtt gcgggtcacc gtctccgccg
     5101 aggagatgag gggctcggtt atgtaatagg cgacgatgac cagccccccg gagatgagca
     5161 gggaggcggg gatgccgacc aacggctcta tctccctccg gaccttcagg cggtccatgg
     5221 aatatatgag gaagcggggg atgaccaccc ccttcaccac cagggtgagg ccggcgacca
     5281 cgtagatgtg ctccgagccc gtatagtgcg ccagcacggc ggcg
//
LOCUS       DAOSYN010000083         5301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_12997_length_5301_cov_4.446054, whole genome shotgun sequence.
ACCESSION   DAOSYN010000083 DAOSYN010000000
VERSION     DAOSYN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12997_length_5301_cov_4.446054"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLJ11_04570"
     CDS             <1..782
                     /locus_tag="PLJ11_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOO03979.1"
                     /translation="NSMLRFIGNGSMDMTRMRFAQNFPKRVFQRYDPITDKDREKIIH
                     FANSVNDSDWQLMLGYGITIIGICTGLRPKELRLANNGDLDLDRGIIHAEHVKGEGKY
                     GEPRDTGIHPDGIPFLMRYVKVRAQKISKEAPTCEALFPAIQNIKKGGDGYFSQNSLT
                     VLRAKVIAQTGVRYDSRACRRTFGQKNVDMGVPIDSVSRMMGHSTTKTTEKYYCRKTT
                     ESAISEAQKVWGNAPKPDKVASSMKVKNPLIDEKFSITGYG"
     gene            complement(783..857)
                     /locus_tag="PLJ11_04575"
     tRNA            complement(783..857)
                     /locus_tag="PLJ11_04575"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(822..824),aa:Gly,seq:tcc)
     gene            complement(1004..1180)
                     /locus_tag="PLJ11_04580"
     CDS             complement(1004..1180)
                     /locus_tag="PLJ11_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03980.1"
                     /translation="MRRNEDPLSEQTYDGHAIEWMKTHTIRDLKLMAFEMDHQGQETT
                     ELWDLINDLKIMGR"
     gene            1419..>5301
                     /locus_tag="PLJ11_04585"
     CDS             1419..>5301
                     /locus_tag="PLJ11_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO03981.1"
                     /translation="MDGLLDAEVRAIKKAVRSLDAADKHIQAKEAEKAIAEANRAKAA
                     LEGLDPKTVSKLDEYRGTLSRLATAYLDLGATIKGMEVLDRFTKAFPEDVPGAKLRIA
                     ALRRLGRTEEALAMLDGLVATHPNDLDAHLQRKDLLLGMGRRGEALQALFKALEADPL
                     NEGTYDLILEMTDDRPLWLGRKASALIQKDRAEAALFELDTALGLAPQDLSLMMLKVK
                     ALEALGQQEEAEDLLDEVLQRDPTNKEANLAMARKAKADGKGTEAVGHYKNVLRSDPE
                     CTEAWAEVAALLQSLGRSEESLRCYDHLKEVMPDDLPTLKGRMSVLAAMKDLPGLDAA
                     AKDVLRVAPNDAAAHLSIIDTFLGLGAEEEADAFLENALREFPRNLEVLDRRRALLSK
                     RKRYQEAIALCEAQLAIKADHLPAFQDLGQAQAGLGNVTEAIRTFERGLRIWPEDLMM
                     LEALRECFKRTGRDKDVADTADRLLRLRPNDKAALFDRAVALDRLGRKEEAVGLYAQV
                     LALDPNDVDASMGIAVALFSLERFEEALARAVQGALHDPGRLVLWRVQADSLFMLKRF
                     EEAVKAYERAIAISPQEQRLLYQKGLSLESLRRFEEALVSYDQALALDPKDKNVWISK
                     GIALEWLERHEEALACYDQALALDKEGRFVHARRGQVLAKLSRHEEAIASFDKALEMG
                     PKDLEVLTAKKGSLKALGRNEDIVKVCDRIVKIEPKNRLAWIDRGMAQFRLGSFAEAV
                     RSYDRALEVEPKDMGVLQLKRAALVAKGDQEEVAKVCDEILRIDPRNKSALIDKATAL
                     ERLGRLEEALATFNAALMLDEANAQLHKDKGRVLAALGKYGEAAEAYDLAFRLDHDPD
                     ALFNKGRALLMMRNYDLALDAFDQCIALDGSVAHFHSDRGRALASLNRLEDAVSAFDK
                     ALTLDRNDAQTWKYKGNALFRLGQLENALVCFNRALDLGADEFAIFKMRGRAMEELGR
                     LEDAMDAYEKALAMEPNEPSVLEGMALLEDKMGRPERALELLDRSLGLDPRNRHGWME
                     RADIAERLKRDEEVLKSYDNAIGLDPSDPAAWNGKGFALLRLGRFDQARRGFEKALEL
                     SPNMPSALEGLRMADGKQRERQVAEMAGKVLELQYRNGRRMSKEEVFREANVPYHMLD
                     EVFAFLEQREYVDPSQLSEGEFASLEMQARTALLMYYRSARSGQGGLTLSDVYSSLPE
                     RDIAQAKRVLGYIEAVNDIDFTYVMPDPETERLLRTALNMPEEKRTLFSLMEGLNIGV
                     YQARNVLAIISSLKGGERPAATPRSKGQRAPP"
BASE COUNT         1140 a         1557 c         1733 g          871 t
ORIGIN      
        1 tgaacagtat gctacgtttc atcggcaacg gttcaatgga catgacgcga atgcggtttg
       61 cccaaaactt cccgaagcgc gtgttccaga ggtatgaccc catcactgac aaggacaggg
      121 aaaagatcat ccatttcgcc aatagcgtga acgattcaga ttggcaactg atgctaggtt
      181 atggaataac cataatcggt atctgtactg ggctgagacc gaaagagctg aggttagcga
      241 acaatggaga tctcgaccta gataggggaa tcattcacgc agagcatgtc aagggcgagg
      301 gcaagtacgg ggaacccaga gacaccggga ttcatcctga tggcattccg ttcctaatgc
      361 gatacgttaa ggtcagagcc caaaagatat cgaaggaagc tccgacatgc gaagcgctct
      421 tccctgccat acaaaacata aaaaagggtg gtgacgggta cttctcgcag aacagcctta
      481 ccgtgctcag agcaaaggta attgctcaga ccggggttcg atatgacagc cgcgcttgtc
      541 gtcgcacctt tggtcaaaag aacgtcgata tgggcgtccc gatcgattcc gtgtcccgca
      601 tgatggggca ctcgaccacg aagaccacgg agaaatatta ctgccggaaa acgaccgaat
      661 cagctatctc ggaagcacag aaagtgtggg gcaatgcacc gaagcccgac aaggtagcca
      721 gttcaatgaa agtcaaaaac cctctgatcg atgaaaaatt ttcgattact ggatatggtt
      781 aatggtgcag gtgtcgggat tcgaacccga gtatttagct tggaaggcta aaatcctgac
      841 cgctagatta cacctgcgcg gtgcgctcat aataaggagt ccgtatttta acccttccat
      901 atcggaggac caactggaag cggaattgaa aataaaggtt gaggctagtg gaaagtgtaa
      961 gggggggggt ctttccacca gggccgttcg ggaacgtatg agcctagcgg cccatgatct
     1021 tcaggtcatt gatgaggtcc cacagctccg tggtctcctg gccctgatgg tccatctcaa
     1081 aggccatcag cttcaggtcc cttatggtgt gggtcttcat ccactcaatg gcatgaccat
     1141 cgtaggtctg ttccgagagc ggatcctcgt ttcgcctcat ttgcatccca tccgtccgac
     1201 cttcgtcaga caataataga cattacgtca cacaatataa gtaattttcc atacgaatag
     1261 ccagtattga aaatatccgt ttccgatcga agcttcgatg gcaataattg tcagacaaac
     1321 agtaaataac tgtatgacat tggaaacgaa gaggccttga caaacatatt aattgtctgg
     1381 gctccattca agtcaggata ggtgatcgga tgggggtgtt ggacggcttg ttggatgcgg
     1441 aggtgagggc catcaaaaag gccgtgagat cgttggacgc tgccgacaag cacatccagg
     1501 ccaaggaggc ggagaaagcg atcgccgagg ccaacagggc caaggccgcc ctcgaaggac
     1561 tggaccccaa gaccgtatcc aagctggacg agtaccgcgg tacgctttcc aggctggcga
     1621 cggcctacct cgacctgggc gccaccatca agggaatgga ggtgctggac cgcttcacga
     1681 aggcgttccc agaggacgtg cctggcgcca agctcaggat cgccgccctg cggaggctgg
     1741 gaaggacgga ggaggccttg gctatgttgg atggattggt cgccacccat ccgaacgatc
     1801 tggacgccca tctgcaaagg aaggacctcc tgctcggcat gggaaggcgg ggggaggccc
     1861 tgcaggccct gttcaaggcc ctggaggccg acccgttgaa cgaggggaca tacgacctca
     1921 tcctggagat gacggacgac cggccgcttt ggctcggcag gaaggcgtcc gccctgatcc
     1981 aaaaggacag agcggaggcc gcgctcttcg agctggacac ggccttggga ttagccccac
     2041 aggacctgtc cctgatgatg ctcaaggtga aggccctgga ggccctcggc cagcaggaag
     2101 aggccgagga cctgctggat gaggtcctgc aaagggaccc cacgaacaag gaggcgaacc
     2161 tggccatggc tcggaaggcc aaggccgacg gtaagggcac cgaggcggtg gggcactaca
     2221 agaacgtcct acgctcggac ccggaatgca cggaggcctg ggccgaggtg gccgcccttc
     2281 tccaatccct agggcgctcc gaggagagcc tgcgctgcta cgaccatctg aaggaggtca
     2341 tgccggacga cctccccacc ctcaagggcc gcatgagcgt cctggcggcc atgaaggacc
     2401 tccccggcct ggacgccgcg gccaaggatg tgctcagggt cgccccgaac gatgcggccg
     2461 cccatctctc gatcatcgac accttcctgg ggctgggggc ggaggaggag gccgacgctt
     2521 tcctggagaa cgccttaagg gaattccccc ggaacctcga ggtccttgac cgacgccggg
     2581 cgctcctctc caagcgcaag cggtaccagg aggcgatcgc cctgtgcgaa gcccagctcg
     2641 cgataaaggc cgaccacctg ccagcgttcc aggacctggg ccaggcccag gcggggctgg
     2701 ggaacgtcac cgaggccatc aggaccttcg agcgcggcct gaggatatgg cccgaggacc
     2761 tcatgatgct cgaggcgctc cgggaatgct tcaagcgcac cggccgggac aaggatgtgg
     2821 cggataccgc cgaccggctg ctgcgtctgc gcccgaacga caaggccgcc ctcttcgaca
     2881 gggcggtggc cctcgaccgc ctcggccgga aggaggaggc cgtcggtctc tacgcccagg
     2941 tcctggcact ggaccccaac gacgtggacg cgtccatggg gatagccgtc gcgctctttt
     3001 cgctggaaag gttcgaggag gcattggcaa gggcggtgca gggcgccctc cacgacccgg
     3061 gcaggctggt cctgtggcgc gtccaggccg attccctgtt catgctcaag cggttcgagg
     3121 aggcggtgaa ggcctacgag agggcgatcg ccatctcccc gcaggagcag cggctgctct
     3181 accagaaggg gctttccctg gaaagcctgc gtcgcttcga ggaggccctc gtaagctacg
     3241 accaggccct ggcgctggac cccaaggaca agaacgtctg gatcagcaaa ggcatcgccc
     3301 tggaatggct ggagaggcac gaggaggccc tggcctgcta cgaccaggcc ctggctttgg
     3361 acaaggaggg gcgcttcgtg catgcccgcc gcggccaggt gctggccaag ctgtcccggc
     3421 acgaggaggc catcgcctcg ttcgacaagg ccctggagat ggggcccaag gacctggagg
     3481 tgcttacggc caagaagggc agcctgaagg cgttggggcg gaacgaggac atagtcaagg
     3541 tctgcgaccg catcgtgaag atcgaaccga agaacaggct ggcctggatc gaccggggca
     3601 tggcccagtt ccgattgggc agcttcgccg aggccgtccg ttcctacgac cgggcgctgg
     3661 aggtggagcc caaggacatg ggcgtcctgc agctgaagcg ggcggcgctg gtggccaagg
     3721 gggaccagga ggaggtggcg aaggtctgcg acgagatcct caggatcgat cccaggaaca
     3781 agtccgccct catcgacaag gcgacggcgc tggagaggct ggggaggctg gaggaggcgc
     3841 tcgctacctt caacgcggcc ttgatgctcg acgaggcgaa cgcgcagctg cacaaggaca
     3901 agggcagggt gctggccgcc ctcggcaagt acggcgaggc ggccgaggcc tacgacctcg
     3961 cgttcaggct cgaccacgac cccgacgcgc tgttcaacaa gggccgggcg ctcctcatga
     4021 tgcgcaacta cgacctggca ttggacgcct tcgatcaatg catcgcactg gacggctcgg
     4081 tcgcccattt ccatagcgac cgcgggcgcg ccctggcatc gctgaaccgc ctcgaggacg
     4141 cggtgagcgc cttcgacaag gcgctcaccc tggaccgcaa cgacgcccag acctggaagt
     4201 acaagggcaa cgccctgttc cgcctggggc agctggagaa cgcgctggta tgcttcaata
     4261 gggccttgga cctgggggcg gatgagttcg ccatcttcaa gatgcgcggc cgggccatgg
     4321 aggagctggg gcgcctcgag gatgccatgg acgcctacga gaaggcgctg gccatggagc
     4381 ccaacgagcc ctccgtgctg gagggcatgg ccctgctgga ggacaagatg ggccggccgg
     4441 agagggccct agagctcctg gaccgctcgc tgggcctcga tccccgcaac cgccacggat
     4501 ggatggagag ggcggacatc gcggagaggc tcaagcggga cgaggaggtc ctgaagagct
     4561 atgacaacgc catcggtctc gacccctcgg accccgcggc ctggaacggc aagggcttcg
     4621 ccctgctgcg cctcgggcgg ttcgaccagg cccggcgcgg gttcgagaag gccctggagc
     4681 tcagcccgaa catgccttcg gccctcgagg ggctgcgcat ggcggacgga aagcaacggg
     4741 aaaggcaggt cgcggagatg gcggggaagg tgctggaact ccagtaccgc aacggccggc
     4801 ggatgagcaa ggaggaggtg ttccgggagg ccaacgtccc ctaccacatg ctggacgagg
     4861 tcttcgcctt cctggagcag cgggagtatg tcgatccgtc ccagctgtcc gagggggagt
     4921 tcgcctccct ggagatgcag gcccgcaccg ccctcctgat gtactaccgt tcggcgcgca
     4981 gcgggcaggg ggggctcacc ctctcggacg tctattcctc cctcccggaa agggacatcg
     5041 cccaggccaa gcgcgtcctc gggtacatcg aggcagtgaa cgacatcgac ttcacctacg
     5101 tcatgccgga cccggagacc gagaggctgc tccgcaccgc gctcaacatg ccggaggaga
     5161 agcgcaccct gttctccctg atggaaggat tgaacatcgg cgtgtaccag gcgcggaacg
     5221 tccttgccat aatcagctcc ctgaagggcg gggagcggcc ggcggcgacg ccccgctcca
     5281 aggggcaacg ggccccgccc g
//
LOCUS       DAOSYN010000084         5300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_13000_length_5300_cov_4.374833, whole genome shotgun sequence.
ACCESSION   DAOSYN010000084 DAOSYN010000000
VERSION     DAOSYN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13000_length_5300_cov_4.374833"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..394
                     /locus_tag="PLJ11_04590"
     CDS             173..394
                     /locus_tag="PLJ11_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03982.1"
                     /translation="MFPSKRTRLERKIKELNALMAEYRDELEETERRFRRREIGRDEL
                     DRITARNKAKMEGITERIRAARAELDGLK"
     gene            1226..2806
                     /locus_tag="PLJ11_04595"
     CDS             1226..2806
                     /locus_tag="PLJ11_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOO03983.1"
                     /translation="MVAEDENVSREMAQVVAKLQDAIGRENVKTSKMERLLYSHDLAP
                     LPNLAQIAFKNIPDVVVRPHTTEDVVKIVKIAAEDKVPITPRGASTWGLGGSTPVFGG
                     ILIDMSGGMNKILEINRENLYVKAQAGCTWKEVYDACMEQGLLLGGYPSSFPSATLGG
                     WISVNGVGVGSMKYGTSGDLVRNMEVVMPDGTVVQTGYDLIVDNETGYNLNRLIVGAE
                     GTLAHICTVTFKLEPAPEVMRSLTYSFPTLKEAGKPLMEICRSRVRPLHIGFSDHNHF
                     DLLRKAGRHTLPEGHNVLNIQLEGDKEIVAWEEKKIDEIMAKYGGKKLPDEVAAHEWE
                     ERCYEYRCREIGIGSIPGEAVMPLNTFEETVDACYNLLKELKLTGAIIGSIVDRNTVM
                     FMPYYLFNPDELQNLTSFGFNAKFANYALSIGGRPLGFGAFFASNLDAIRGTGAKYIR
                     DIKRLIDPDDIMNPGKLTGTTLRYGIRIPPALFNLGMGAMGVIKRAFPTEDEVAERQE
                     AYAQERANKARDGRHKGH"
     gene            complement(3022..3144)
                     /locus_tag="PLJ11_04600"
     CDS             complement(3022..3144)
                     /locus_tag="PLJ11_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOO03984.1"
                     /translation="MEVDEKSAKWTSEYKGKRYYFCAPGCKKKFDASPDKYVKG"
     gene            complement(3159..>5300)
                     /locus_tag="PLJ11_04605"
     CDS             complement(3159..>5300)
                     /locus_tag="PLJ11_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOO03985.1"
                     /translation="ERLAVRYDPQRVRVAQIKKAVEDAGYEVIEAQTLDVEREMREKE
                     TRRQRRMLLLSVGLAVPTMAIMLLMDLTSIAHHFLMEWGGLLMFALATPVQFIAGRQF
                     YVGAYKALRNRTANMDTLIAVGTSAAYFYSVAVVLFPGAVPVDHVYFDSSSMIIALIL
                     FGKYLEAKAKGSTSEAIKRLIGLQAKTARVVRDGEEVEMPVDDLDVGDVMVVRPGEKV
                     PTDGVVIEGRSSVDESIITGESLPVPKEEGSEVIGASINRNGMLKVRATRVGKDTALA
                     QIVKLVEDAQVAKAPIQRLADRVAAVFVPTVIVIALTAFALWYVFGVPALAPEEPRFA
                     FSLTIFITVLVIACPCALGLATPTAIMVGTGKGAELGILIKGGDALEVAGRVTTVVFD
                     KTGTLTKGQPEVMEILRFDESCPDMFVMAASVEAGSEHPLGEAIVRRAKADGIPLMDI
                     SGFESLAGKGVRATVNGRLVTVGNRALLEELNVPLGERETVVAALEERGMTVTMVLLD
                     GKPCGAIGIADAIKPTSAETVSELKGMGIDVVMLTGDNRRTAEAVARRLGIERVLAEV
                     LPGDKAKEVAELQSSGRVVAMVGDGINDAPALAQADVGIALGSGTDVAVEAADIVLIK
                     DDPRDVVAAVQLSRQTMRKIRQNLFWAFAYNTAGIPIAAGALYPFFGILLSPIIAAGA
                     MAFSSVSVVTNAALLKRYVPEIKKKEVIERP"
BASE COUNT         1085 a         1667 c         1537 g         1011 t
ORIGIN      
        1 ggggaccgca tttggaaagg ggcaagggcg ataggcggcc ggagcgctct agaccacctg
       61 gccccactgg caggccaagg gggtcgcgca tgggccaagg agagcattgc cgtccagata
      121 gtgggtgcgg tcccaaaacc ttttattcgc tcatttaatt gttttttata cgatgttccc
      181 gagcaagcgc accaggctgg aaaggaagat caaggaattg aacgccttga tggccgagta
      241 ccgggatgag cttgaggaga ctgaaaggcg gtttcggaga agggagatcg gacgggacga
      301 actggaccgc atcacggcgc ggaacaaggc caagatggag gggatcaccg aaaggataag
      361 ggccgcgagg gcggagctgg atggattgaa atgaccctct acttttttcg tatccatcga
      421 taaaaggaac ttattaatag tcagcagagc aacataggat attgcccgtg gaatggagag
      481 ggcaggacac gtgaacggca ataacgttca cccaagcctt catcggcctt cgcggtcgac
      541 cgcatcgcgg tccgagcaga tgatcactgc cctggacgga gcggcttccc ccctgcgccc
      601 tacgtgcgat aggcgggaga tgaaggggtg gtcgcccgag gaacagctcc attttgagga
      661 atgggtcgga ccaagggtca ccttcctcct cccctccgcc cgggcactct tgagctatat
      721 catttttcac ccttattcat ttttatttat ataaaataaa taatattctg gatatctcat
      781 taaatatgca aagttatata tactaggcaa tacaataggt attattgccc gagtcggaaa
      841 ggctgttggg aaacctgaat ggatccagca tgatttgagg atcatgccca tcggaaaagg
      901 tcctattcgc gtgctccttc gggagcaggt tcgcccacgc acttcccggc tataggcggg
      961 aaagcagcga ggcggatggc gatcgattcc ctccgggggg tcgaaagcta ggtaggacgt
     1021 catcgaggtg gcgcatactc catctgcaga tgggagggcg ccaagcaggg acgaagtacc
     1081 ttcgagcgcc gaactcgggc acccttctcc attttttcct ttcgaccaat gcacattttt
     1141 catgtatggc tcgggaatag gtttaaaata cttcacggac ttcagggtac aatctcattt
     1201 atatcctggt gatggaggtc ttcacatggt agctgaggat gaaaacgtaa gcagggaaat
     1261 ggcccaggtc gtggccaagt tgcaggatgc gatcggtcgg gagaatgtca agaccagcaa
     1321 gatggagaga ttgctgtaca gccatgacct ggccccgctc cccaacctcg cccagatcgc
     1381 cttcaagaac attcccgacg tcgtcgtgcg cccccacacc acggaggacg tggtcaagat
     1441 cgtgaagatc gcagcggagg acaaggtccc gataaccccc cgcggggcat ccacctgggg
     1501 gctgggaggc tccacccccg tctttggcgg gatcctcatc gacatgtccg gcgggatgaa
     1561 caagatcctc gagatcaaca gggagaacct ctatgtcaag gcgcaggccg gatgcacctg
     1621 gaaggaggtc tacgatgcgt gcatggagca aggtctcctc ctcggcggct accccagctc
     1681 cttcccctcg gccaccttgg gcggctggat atccgtgaac ggggtcggcg tcggctccat
     1741 gaagtacggg acctcgggcg acctcgtccg caacatggag gtcgtgatgc ccgatggcac
     1801 agtcgtccag accggatacg atctcatcgt ggacaacgag acaggctaca acctcaaccg
     1861 cctcatagtg ggcgcggagg gcaccctcgc ccacatctgc acggtcacct tcaagctcga
     1921 gcccgccccc gaggtcatgc gatccctgac atactccttc cccaccctca aggaggcggg
     1981 gaagcccctc atggagatct gccgctcccg cgtcaggccc ctgcacatcg ggttcagcga
     2041 tcacaaccac ttcgacctgc tccggaaggc cggcaggcac acccttccag agggccataa
     2101 cgtcctaaac atccagctgg agggggacaa ggagatcgtc gcctgggagg agaagaagat
     2161 cgacgagatc atggcgaagt acggcggcaa gaagcttccc gatgaggtcg ctgcccatga
     2221 gtgggaggag cggtgctatg agtatcgctg ccgcgagatc ggcataggga gcatcccggg
     2281 ggaggctgtg atgccgctga acacgttcga ggagacggtg gacgcctgct acaacctcct
     2341 gaaggaactg aagctcacgg gagccataat cggttccatc gtcgaccgca acacggtcat
     2401 gttcatgccc tattacctct tcaacccgga cgagctgcag aacctcacct ccttcggctt
     2461 caacgccaag ttcgccaact atgccctgtc gatcggcggg cgcccgctgg gattcggagc
     2521 gttcttcgca tccaatctcg atgcgatcag ggggacgggg gcgaagtaca tcagggacat
     2581 caagaggctc atcgaccccg atgacatcat gaaccccggg aagctcactg gcacgaccct
     2641 gcggtatgga atcaggatcc cgccggccct gttcaacctg ggcatggggg caatgggggt
     2701 cataaagcgg gcgttcccca ccgaggacga ggtggcggaa cgacaggagg cctatgccca
     2761 agagcgcgcg aacaaggccc gggacgggag gcacaagggc cactgatcgt tataggcgcg
     2821 atcccctttc ttatcgaagg gtccccggtt gagcgggggg cctttcggca ctttcccctt
     2881 ccatcgatcc cccggtccaa ctatctgaaa agggcttcct tcgcattcag actaccatgc
     2941 cgctcagccc gatggcatgg cggtctggat ggcatgctca tctctcagag ccgagtataa
     3001 aggatgggaa gtggtttctt ttcacccctt tacgtactta tcagggctcg catcgaactt
     3061 cttcttacag ccaggggcgc agaagtagta tcgcttgccc ttgtactcgg aggtccattt
     3121 cgcgctcttc tcatcgacct ccatcttgca tatcgggtct acggccgttc aatcacctcc
     3181 tttttcttga tctccggaac atagcgtttc agcagcgcag cgttcgtcac cacggacacg
     3241 gacgagaagg ccatggcccc agccgcgatt ataggggaga gcaggatccc gaagaagggg
     3301 tagagtgcac cggcggcgat ggggatcccg gcggtgttgt aggcgaaggc ccagaatagg
     3361 ttctgtcgta tcttgcgcat ggtctgccgg ctcaactgca cggccgccac gacatcacgc
     3421 gggtcatcct tgatgaggac tatgtccgcc gcctccaccg caacatccgt gcctgagccc
     3481 agggcgatcc ccacatcggc ctgcgccagc gcaggggcgt cattgatgcc gtcgccgacc
     3541 atggccacca ccctgccaga tgactgcagt tcggccacct ccttcgcctt atcccccggg
     3601 agcacctccg ccagcaccct ctcgatgccc agccttctcg cgaccgcctc cgccgtcctc
     3661 cggttgtccc ccgtcagcat caccacgtcg atgcccatcc ccttcagctc ggatacggtc
     3721 tccgccgagg tgggcttgat cgcatccgcg atgcctatgg ccccgcatgg cttcccatca
     3781 aggagcacca tcgtgaccgt cataccccgt tcctccaagg cagcgacgac cgtctcccgc
     3841 tcccccaacg gaacgttcaa ttcctcgagg agggcgcggt tacctacggt caccaggcga
     3901 ccgttcacgg tcgccctcac cccctttcca gccaggctct cgaacccgct gatatccatg
     3961 aggggtatcc catcggcctt cgccctgcgg acgatggcct ctcccaacgg gtgctcggac
     4021 cccgcctcga cgctggccgc cattacaaac atgtccgggc acgattcatc gaagcgtagg
     4081 atttccatca cctccggctg ccccttggtc agggtccccg tcttgtcgaa gacgaccgtg
     4141 gtcacccttc cagccacctc gagcgcgtcc cctcctttga tgagaatgcc gagctcggcc
     4201 cccttccccg tccctaccat gatggccgtc ggggtggcca atcccagggc gcaggggcag
     4261 gcgatcacta ggaccgttat gaagatggtg agcgagaacg cgaacctcgg ttcctccggg
     4321 gcgagggccg ggaccccaaa gacgtaccag agggcgaagg cggtcagggc gatcacgatc
     4381 acagtcggta cgaacacggc cgccacccgg tccgccagcc tctggatggg ggccttggcc
     4441 acctgggcgt cctccaccaa tttgactatc tgcgccaggg cggtgtcctt accgaccctg
     4501 gtggccctga ccttgagcat tccgttcctg ttgatgctcg ccccaatgac ctccgagccc
     4561 tcctccttcg gaacaggcag gctctcccct gttattatgc tctcatccac cgaggaacgc
     4621 ccctcgatga ccaccccgtc ggtcgggacc ttctcccccg ggcgaaccac catcacgtcc
     4681 ccgacgtcaa ggtcgtccac cggcatctcc acctcctccc cgtcccggac gacccgggcg
     4741 gtctttgcct gaagcccgat gaggcgcttg atcgcctcgg acgtgctccc cttggccttg
     4801 gcctccaggt acttgccgaa gaggataagg gcgatgatca tcgacgagct gtcgaagtag
     4861 acatggtcca caggcaccgc tcccgggaag aggaccacgg ccacgctgta gaagtaggcg
     4921 gcggaggtcc ccacggcaat gagcgtatcc atgttcgccg tgcggttgcg gagggccttg
     4981 taggcgccaa cgtagaactg gcggccggcg atgaactgca ccggcgtggc cagggcgaac
     5041 atgagcaggc ccccccattc catcaggaag tgatgggcga tggacgtgag gtccatcagc
     5101 agcatgatgg ccatcgtggg gaccgcaagc ccgaccgaca ggagcaacat ccgcctctgc
     5161 ctacgggtct ccttctcccg catctccctc tccacgtcca gggtctgggc ctcgatgacc
     5221 tcgtaccccg catcctccac cgccttcttg atctgggcga cccggacccg ctgaggatcg
     5281 tagcggacgg cgagcctttc
//
LOCUS       DAOSYN010000085         5280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_13072_length_5280_cov_4.324402, whole genome shotgun sequence.
ACCESSION   DAOSYN010000085 DAOSYN010000000
VERSION     DAOSYN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13072_length_5280_cov_4.324402"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..915
                     /locus_tag="PLJ11_04610"
     CDS             13..915
                     /locus_tag="PLJ11_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO03986.1"
                     /translation="MCEGHRLCAGCAEPIIARQVLMATDKPVVVANATGCFEVSTTIY
                     PFTAWNVPWIHTAFENAPATISGVEAAYNSLKRQGKLEGEIKFVAFGGDGATYDIGLQ
                     SLSGAIERGHDFLYVCFNNEAYMNTGIQRSGATQKGASTTTCPAGSCIPGKREFPKDL
                     TRIIIAHDIPYAAQASPHNWKDLIEKSRKGIETHGPAFINVVSPCPRGWRHDSSQTIA
                     ISKLAVETCVWPLYEYENGEWRLTGESLRIAEGSKEKRPVSEWLSSQGRFKHLTKGKF
                     TSVADEIQANVDKKWGELLKLSKM"
     gene            complement(960..1667)
                     /locus_tag="PLJ11_04615"
     CDS             complement(960..1667)
                     /locus_tag="PLJ11_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO03987.1"
                     /translation="MRIVYGPIKSLGLGKVVAVDPISRSPKVCTLNCVYCRLGRGGMM
                     LLDRTAFIDCSSLMDQVGECLYREECETVMFKGTGEPLLASNIFDMARRLRDGSDKKV
                     ALLTNCTLLKDPQVLGQLDAFDIIIAKLDAASEETFQAVNRPHPSLRFQDVLEGIRSA
                     RERFDGSFRIQITLVRENLSEVDDLSQLCREICPDYVYLNCPEGCDPFHMVSRKDMQA
                     AMDRFFGIRCVSSNAKE"
     gene            complement(1720..1944)
                     /locus_tag="PLJ11_04620"
     CDS             complement(1720..1944)
                     /locus_tag="PLJ11_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOO03988.1"
                     /translation="MCLAMPAKVLSIDGDEAEVDFGGAIRRTNVSMVDAKVGEYVIIH
                     AGFAIQKVDEEEALETLQLWSEFLGGSEEA"
     gene            complement(1950..2315)
                     /locus_tag="PLJ11_04625"
     CDS             complement(1950..2315)
                     /locus_tag="PLJ11_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HOO03989.1"
                     /translation="MPGMGRGVNPRQMKQAMKRMGISQEEMQGVEEVVIRTADKDYVI
                     KEAAVTCITMQGQKTYQVAGEMEVRPRQKAVQEAPDIPEEDVQLVMSQTGASKERALH
                     ALKECDGQPAEAILKLMSE"
     gene            complement(2380..3057)
                     /locus_tag="PLJ11_04630"
     CDS             complement(2380..3057)
                     /locus_tag="PLJ11_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOO03990.1"
                     /translation="MCGRFSLGLVYGFSTRFGVPEVPELRPRYNIAPFQQVLIIVGGE
                     AKSVKMMRWGLVPHWAKGEEFGLKLINVRSESVMEKPMFKPLLNRQRCLVPATGFYEW
                     QRQGPRKRPYHVRVKGQEYFAMAGIYDTWSKEGKDLVTFSILTTAANATIAPIHDRMP
                     VILRPEREAEWVSKEVLSADTLSEMFEPLPAASMDAYPVSDMVNDSHLDHPDMVVPQR
                     MQQATLF"
     gene            complement(3127..4470)
                     /locus_tag="PLJ11_04635"
     CDS             complement(3127..4470)
                     /locus_tag="PLJ11_04635"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOO03991.1"
                     /translation="MATDKDYAEMKEDILKRVRKENVKFIEMQFSDILGTVKSVSIPT
                     TRVESVIDDGVYLDGSSILGYATIEESDMRANPILDSFQIYPWVDSGVKTARFMCTIS
                     DHSGNRFKGDPRWALEKMVDRVKEMGYEFNVGPEFEFFMFPMQNGIPVLVPADAGGYF
                     DLMPQDKGEVARKEMVLAFDTLGYDMEASHHEVAPGQVEIDLRYQSALTMADRMLTLK
                     YGVKCIAQKYGLYASFMPKPLYGENGSGMHVHMSLSNKDRNAFYDPEGKWGLSEMAMN
                     FMGGLLAHAKENCAILASYVNSYKRLVPGYEAPCYISWANMNRSALLRVPAGRGMRTR
                     VEQRNPDPAGNPYLQFAVMLAAGLDGIKNRLSPGEPIEKDIYHMSPEDRRRHRIEDLP
                     MNLGDALDVLAESKVMKEALGEHIFNHYLHIKGLEWDEYRKHVTDWEIQRYLRVL"
     gene            complement(4608..>5280)
                     /locus_tag="PLJ11_04640"
     CDS             complement(4608..>5280)
                     /locus_tag="PLJ11_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOO03992.1"
                     /translation="PRAKLDRISERMVRVVSIGEHDHAACAGVHLGDAREIGMLLVNK
                     FTSAKPAGDWEIEFLVGPEAVRAAVDLSISALMLGERMGALPQDSLTAFDNRERELNR
                     AREALKVYGRTALASLRPRELGPWKVFTGSFLALDRRLVMEWANELVAIGGSVAVLVC
                     QDERTFLVLARSPDVDIDCVTILREALAPYGGRGGGKPAFASGGTSDQVDPGALIDGV
                     LRLLK"
BASE COUNT          976 a         1716 c         1509 g         1079 t
ORIGIN      
        1 accgaggaga ggctgtgcga ggggcatcgc ctctgcgccg ggtgcgcgga gcctatcatc
       61 gcccgccagg tgctgatggc gaccgacaag cccgtggtgg tcgccaacgc caccgggtgc
      121 ttcgaggtgt ccaccaccat ctatccgttc accgcttgga acgtgccatg gatccatacc
      181 gcctttgaga acgccccggc cacgatcagc ggggtggagg ctgcctacaa ctccctgaag
      241 cgccagggca agctcgaggg agagatcaag ttcgtggcct tcggcgggga cggggcgacc
      301 tatgatattg gcctgcagtc cttgtccggc gccatagagc gcgggcacga tttcctgtat
      361 gtctgcttca acaacgaagc gtacatgaac accgggatcc agaggagcgg ggccacccag
      421 aagggcgcat cgaccaccac ctgtccggcc gggagctgca ttccgggcaa gcgggagttc
      481 cctaaggacc tcacccgcat cataatcgcc catgacatac cctatgccgc tcaggcatcg
      541 ccgcacaact ggaaggacct catcgaaaag tcccgcaagg gcatcgagac ccatggaccg
      601 gccttcatca acgtggtctc cccctgcccc cggggatgga ggcatgacag ttcccagacc
      661 atcgccatct ccaagctggc ggtggaaacg tgcgtctggc ccctctacga gtacgagaac
      721 ggcgagtgga ggctgaccgg ggagtcgctg cgcatcgccg aagggtctaa ggagaagcgg
      781 ccggtgtcgg aatggttgag ttcccagggc cgcttcaagc acctgaccaa gggcaagttc
      841 acctccgtgg cggacgagat ccaggccaat gtggacaaga agtggggcga actgctcaag
      901 ctcagcaaga tgtgagcaaa ccccttcccc ttctttttac cctctttttt accttcaact
      961 cattccttgg cattggagct tacgcacctt atcccgaaga accggtccat ggccgcctgc
     1021 atgtccttcc tgctcaccat gtggaagggg tcgcagccct cgggacagtt cagatagacg
     1081 tagtcgggac agatctccct gcacagctgg gaaagatcat ccacctcgga gaggttctcc
     1141 cggaccagcg tgatctggat gcggaacgag ccatcgaacc gctcgcgggc gctccgtatg
     1201 ccctccagca cgtcctggaa cctcagggat ggatgtgggc ggttcaccgc ctggaacgtc
     1261 tcctcgctcg ccgcatccag cttggcgatg atgatgtcga aggcgtccag ctgtcccagg
     1321 acctgcgggt ccttcagaag ggtgcagttg gtgagcaggg ccaccttctt gtcggagccg
     1381 tcccggagcc ttctggccat gtcgaagatg ttgctggcca gcaggggctc ccccgtcccc
     1441 ttgaacatga ccgtctcgca ctcctcccgg tacaggcact ccccgacctg gtccatcaag
     1501 gaggagcagt cgatgaaggc cgtccgatcg agcaacatca tcccgccccg ccccaaacgg
     1561 cagtacacac aattgagggt acacaccttg ggagaacggc tgatcgggtc caccgccacc
     1621 acctttccca agcccaggga cttgatcggc ccgtatacga tcctcatttg ccttctcggt
     1681 cctcataagg gaagggggta gggcataaat gtttggtgcc taggcctcct cggacccgcc
     1741 caagaactcg ctccacagct gtagggtctc gagagcctcc tcctcgtcca ccttctggat
     1801 ggcgaaccct gcatggatga tgacatactc gccgaccttg gcatccacca tggacacgtt
     1861 cgtcctcctg atcgctcccc cgaagtccac ctcggcctcg tcgccgtcga tggacaatac
     1921 cttggctggc atcgctaggc acatgacgct cactccgaca tcagcttgag gatggcctcc
     1981 gcaggctggc catcgcattc cttcagggcg tgcagcgccc tttccttcga cgccccggtc
     2041 tgggacatca ccagctgcac atcctcctcc gggatgtccg gggcctcctg cacggccttc
     2101 tgacggggcc ggacctccat ctcccccgcc acctggtacg tcttctggcc ctgcattgtg
     2161 atgcatgtca ccgcggcctc cttgatgaca tagtccttgt ccgcggtcct gatcacgacc
     2221 tcctccacgc cctgcatctc ctcctgggag atgcccatgc gcttcatggc ctgcttcatc
     2281 tggcgcggat tgacgcccct gcccattcct ggcatcatga gatcgctcga cggattaatc
     2341 ctaccggtat aaaaacagtt gggatttatc caacgccgtt cagaacaggg tggcctgctg
     2401 catccgttgc ggcacaacca tgtccgggtg gtcgaggtgc gaatcgttga ccatgtcgga
     2461 aacaggatag gcgtccatgc tggcggcggg cagcggttcg aacatctccg agagcgtgtc
     2521 cgcggagagc acctccttgg aaacccattc cgcctccctt tccggacgca gtatcaccgg
     2581 catccggtcg tggatcggcg ctatcgtcgc gttcgccgct gtggtgagga tggaaaaggt
     2641 gaccaggtcc ttcccctcct tggaccaggt gtcgtagatc ccggccatgg cgaagtactc
     2701 ctgccccttt actctgacat gataggggcg tttccgtggt ccttgcctct gccactcata
     2761 aaaaccggtg gccgggacca ggcacctctg gcggttgagc aggggcttga acatcggctt
     2821 ctccatgacc gattccgagc ggacgtttat gagcttgagg ccgaactcct cgcccttggc
     2881 ccagtgcggc accagccccc atctcatcat cttaacgctc ttggcctccc cacccacgat
     2941 gatcagcacc tgctggaagg gcgcgatgtt gtaccgcggc ctcagctccg ggacctcggg
     3001 caccccgaac cgggtggaaa agccgtacac caggccgagg gagaagcgac cgcacatgcg
     3061 aatacgattg aatacagaac ggaaaaaggt ttagtaaggc gttcggaagg gggttgcgag
     3121 atcgcatcat agtacgcgga ggtaccgctg tatctcccag tcggtgacat gcttccggta
     3181 ctcgtcccat tccaagccct tgatgtgcag gtagtggttg aagatgtgct ctcccagcgc
     3241 ctccttcatc accttgctct ccgccagcac gtccaatgcg tcccccaggt tcatcgggag
     3301 gtcctcgatc ctgtgcctgc ggcgatcttc cggggacatg tggtagatgt ccttctcaat
     3361 cggctctccg gggctgagcc tgttcttgat gccgtccaga ccagccgcga gcatgacggc
     3421 gaactgcagg taggggttcc cggccgggtc agggttcctc tgctccaccc gggtgcgcat
     3481 cccgcgcccc gcaggcaccc tcagcagggc gctgcggttc atgttggccc atgagatgta
     3541 gcagggggcc tcatacccgg ggaccaggcg cttgtaggag ttgacgtagg aggccaggat
     3601 ggcacagttc tccttggcgt gggccaatag tccgcccatg aagttcatgg ccatctcgct
     3661 cagcccccac tttccttccg ggtcatagaa cgcgttcctg tccttgttgg acagggacat
     3721 gtgcacatgc atgcccgagc cattctcgcc gtacagcggc ttgggcatga agctggcgta
     3781 caggccgtac ttctgggcga tgcacttcac cccgtacttg agggtgagca tgcggtcggc
     3841 catggtgagg gcgctctggt agcgcaggtc gatctcaacc tggccggggg cgacctcgtg
     3901 gtgggaagcc tccatatcgt acccgagcgt atcgaaggcg aggaccatct ccttcctggc
     3961 gacctcgccc ttgtcctggg gcatcaggtc gaaatagcct ccggcatccg cggggacaag
     4021 gacggggatg ccgttctgca tcgggaacat gaagaactcg aactccgggc ccacgttgaa
     4081 ctcgtagccc atctccttca ccctgtccac catcttctcc agcgcccagc gggggtcccc
     4141 cttgaatcgg ttgccgctgt ggtcggagat ggtgcacatg aagcgggcgg tcttgacccc
     4201 tgagtccacc cagggataga tctggaagga gtcgaggatg gggttggccc tcatgtcgga
     4261 ctcctcgatg gtcgcataac cgaggatgga ggagccgtcg aggtacacgc cgtcgtcgat
     4321 cacgctctcc accctggtgg tcgggatgga cacgctcttc acggtcccta gtatgtccga
     4381 gaactgcatc tcgatgaact tgacgttctc cttcctcacc ctcttcaaga tgtcctcttt
     4441 catctcggcg tagtccttgt cggtggccat tgggaagggg ttacagtgaa gtacattaaa
     4501 tatctttcta agcaacaaat gtgcatcgtt aagcctatag actatgcatt tgcatattct
     4561 atatcacata taatgtacat gtgaatgttt ttttgatgat atttgctcta tttgagcaaa
     4621 cgcaataccc catcgatcag cgcccctggg tccacctggt cggacgtccc tccgctggcg
     4681 aacgccggtt tgccgcctcc ccttcccccg tagggagcga gcgcttccct caggatggtc
     4741 acgcaatcga tgtccacgtc cggggaccgg gcgagcacca ggaacgtcct ctcatcctga
     4801 cagaccagaa cggccaccga gccccctatg gcgaccagtt cgttcgccca ttccatcact
     4861 agcctcctgt ccaaggccag gaacgagccg gtgaacacct tccaggggcc gagctcccgc
     4921 ggacgcaggg atgccagcgc cgtcctgccg tacaccttca acgcctccct ggccctgttc
     4981 agttcccttt cccggttgtc gaaggcggtc aggctgtcct gcggaagcgc ccccatcctc
     5041 tcgcctagca tgagcgcgct tatggagagg tcgacggccg ccctcaccgc ctccggcccc
     5101 accaggaact cgatctccca gtcccccgcg ggcttggcgg acgtgaactt gttgacgagc
     5161 agcatgccga tctcgcgggc gtcccccaag tgcacaccag cgcaggcggc gtgatcgtgc
     5221 tccccgatgc tgaccaccct gaccatcctt tcggatatac ggtccagctt ggccctcggt
//
LOCUS       DAOSYN010000086         5274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_13088_length_5274_cov_4.383982, whole genome shotgun sequence.
ACCESSION   DAOSYN010000086 DAOSYN010000000
VERSION     DAOSYN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13088_length_5274_cov_4.383982"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLJ11_04645"
     CDS             complement(<1..695)
                     /locus_tag="PLJ11_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF108 domain-containing protein"
                     /protein_id="HOO03993.1"
                     /translation="MRLLIIGCGAIGATLAEAIQGMEEFDIFYITDKNEDRARRIVSK
                     CPKAKFISPTDESIKARLKNVDLVVEAASQEAVRHYVPIVLGMGVDVLVMSVGAFADD
                     DTRERCFNVAKRKGGRLYVPSGAICGTDGLHSASADVIQSVELMSIKGPKSFQENEYL
                     LAKGVDVNALKERTILFEGPAREAIQHFPKNVNVSVTISLIGIGLDKTMVKLVCDPKS
                     DCNTHRLEVWGAFG"
     gene            803..1207
                     /locus_tag="PLJ11_04650"
     CDS             803..1207
                     /locus_tag="PLJ11_04650"
                     /inference="COORDINATES: protein motif:HMM:NF001617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOO03994.1"
                     /translation="MRYRTLDHTADVMVEAFGGSLEECFANAAYALTDQIVDVRTVLP
                     REEREIVAEGHDHESMLYNFLSEVLFVFDATGMVFSEFSVTFSDGEVRCLARGERFDA
                     ERHRPKKEVKAITYHMLAVDPVRPSVRVVFDI"
     gene            1291..2409
                     /locus_tag="PLJ11_04655"
     CDS             1291..2409
                     /locus_tag="PLJ11_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOO03995.1"
                     /translation="MDEAFPFEGKVFVSALQGPVTANDIARELTAEVVEDGDQLFEVI
                     RRYEWVLANVLRKEGVKSGDSVRLVLPFLKAYGATDSLLLRLSKQRLRIVPGADKAMR
                     YVQELMSSFILSTSFEHYVGVACDQIGFPFESTFCTRLSMDMLEIEDWEGETLRTLAR
                     EIVRLPPLEVPEGARSIRDLGPDARLALRRLDEIFWTEMTDLSSYQLVSQVSPVGRDE
                     KATSVVEICKRMSVPLEDCMYIGDGDTDARALQVVRRSGGLAVAFNGNLSALQEAEVG
                     TITPNAIVTSILAELFYRGGRDGVLEAIEGWSTEGLRSTGMVHNYLLRELSRTFPEAL
                     PTVRRMSKECLPAMFHEAARMRIQMRRPLPNAPSDEMS"
     gene            2454..2717
                     /locus_tag="PLJ11_04660"
     CDS             2454..2717
                     /locus_tag="PLJ11_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO03996.1"
                     /translation="MTFFLVRMLRHHSRADRSMVPDPEMLDYYFEEYQLGGITRTLIR
                     SDPPSKGVSQWLPLGTEGHKGQDGTAGPVDVWLVHDLLNDLMS"
     gene            2761..3126
                     /gene="cutA"
                     /locus_tag="PLJ11_04665"
     CDS             2761..3126
                     /gene="cutA"
                     /locus_tag="PLJ11_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOO03997.1"
                     /translation="MYCSVLVTAPDLRTARDIGRTMVERGLAACVNIWEVSSIYRWKG
                     EVHEGPEAAMLFKVPREGFDALRSAILEVHPYEVPCIVGYAITAGHPPYLDWISESAP
                     PSGGEAQKPRRTGNDLIGR"
     gene            3145..4185
                     /locus_tag="PLJ11_04670"
     CDS             3145..4185
                     /locus_tag="PLJ11_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO03998.1"
                     /translation="MNDELDQSRPLLLDLLRTLQQCEDRESAIRAVMDTVAGMLRPER
                     LCYLPNKGTPIGDAGAWTSGARLLPDQIVKLLPSKKGFSLAIKEKGLRIGTLCADDVE
                     RPRSLDSMLPILLMVPDALEIAFSNIRALEGLRLSEAKLAQLSESLAVANRILRHDIA
                     NEILVVSSSLDLYKANQRERDLARAQSSLQRMQGIVLKMRELDGFLLSRTEMMPTNLR
                     EVIDKAMPPVEIAYEVVGDGNVLADPAIGAIIENMASNAKRHGMAGHITFTISQGNGT
                     VLLTVRDDGKGIPEEHLPRVFIEGAAFGEARGTGLGLYLIKRTMERYGGSITAGNDPR
                     GGARFELTFQSA"
     gene            complement(4260..4526)
                     /locus_tag="PLJ11_04675"
     CDS             complement(4260..4526)
                     /locus_tag="PLJ11_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOO03999.1"
                     /translation="MERPDLYVVARVLERLWREPSPILRTRLQVAVGTNYDVFSRYLD
                     WMTERGLTALIEEDGHQKVVLTDGGREAYRRLVVWINEVVHGRR"
     gene            complement(4531..>5274)
                     /locus_tag="PLJ11_04680"
     CDS             complement(4531..>5274)
                     /locus_tag="PLJ11_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04000.1"
                     /translation="LFLFSLYIYAQCWEGVEALSSKMRGVGPAEDRGVEELMVDSLSS
                     AYILARDALDVEKGSYQDRGLVLVSLALVAWVVLLALTGPTSGHGHALLLGIGTLIIA
                     GATAGGVLLLRRRCQEDIAREEAWAERILAAMRDREGVSSSVGLLLDICREVPRWLEV
                     RRRGVWNRAPGKTLLIVLLFISGGQIMALYGPGWWGAYIIGAGLIALGTGLFLDESVR
                     ARKEHRRSTEDWDRKLAELYLLLEPGGGR"
BASE COUNT          977 a         1698 c         1658 g          941 t
ORIGIN      
        1 ccgaacgcgc cccaaacctc caggcgatgg gtgttacagt cgctcttcgg atcacacaca
       61 agcttgacca tggtcttgtc cagcccgatg ccgatgaggg atatcgttac ggatacgttg
      121 acgttcttag ggaaatgctg aatggcctcc ctggcgggtc cctcgaagag gatggtcctc
      181 tccttcaagg cattgacgtc cacgcccttt gctagcaggt actcgttctc ctgaaagctc
      241 ttgggcccct tgatggacat gagctcgacg ctctggatca catcggccga tgcggagtgc
      301 agcccatccg tgccgcagat ggcgccagaa gggacgtaca atcgaccgcc cttgcgcttg
      361 gcgacattga agcacctttc cctggtatcg tcatcggcga aggcgccgac gctcatgacc
      421 aggacgtcca cccccatccc cagtacgatg ggcacatagt gccgcaccgc ctcctgcgag
      481 gcggcctcca cgaccaggtc gacgttcttg aggcgggcct tgatgctctc gtcggtcggg
      541 gagatgaact tggccttcgg gcatttggag acgatccttc ttgcccggtc ctcgttcttg
      601 tcagtgatat agaagatatc gaactcttcc attccctgga tcgcctcagc cagggttgcg
      661 ccgatggccc cgcagccgat gatgaggagt cgcatcgggc gactaatcac ataaccccga
      721 tataaccttg tgccccctcc cagcgccccc taccgcttcg ggcgggcatg gggttttaag
      781 cctggccggt gatgacggag cgatgaggta tcgcaccttg gaccacacgg cggacgtgat
      841 ggtggaggcg ttcgggggct ccttggagga atgcttcgcc aatgccgcct acgccctgac
      901 cgaccagatc gtggacgtcc gcacggtgct tcccagggag gagagggaga tcgtggcgga
      961 ggggcacgac cacgagtcca tgttgtacaa cttcctctcc gaggtgctct tcgtgttcga
     1021 cgccaccggc atggtgttct ccgagttctc ggtcaccttc tccgatgggg aggtgcgttg
     1081 cctggcaagg ggcgagcggt tcgatgcgga gcggcaccgg ccgaagaagg aggtcaaggc
     1141 cataacctac catatgttgg ccgttgaccc ggttaggccc tccgtgcggg tcgtcttcga
     1201 tatctgaccc cgaatcgtta gctaaataag gggaaccccc cttatggcac caacatgcga
     1261 cgggaggggg cctgaacgga gttcgacatg atggatgagg cgttcccctt cgaagggaag
     1321 gtgttcgttt ccgccctgca ggggccggta accgcgaacg acatcgcccg ggagctcacg
     1381 gccgaggtcg tggaggacgg cgaccagctc ttcgaggtca tccggcggta cgaatgggtc
     1441 ctggccaatg tcctcaggaa ggagggcgtg aagagcgggg actccgtgcg gctcgtcctc
     1501 cccttcctga aggcctacgg cgcgaccgat tccctgctgc tgcgcctgtc aaagcagcgg
     1561 ctgcgcatcg ttcccggggc ggacaaggcc atgcggtacg tgcaggagct catgtccagt
     1621 ttcatcttaa gtacaagctt cgagcactac gtgggagtgg cctgcgacca gatcggcttt
     1681 ccgttcgaga gcaccttctg cacccgcctg agcatggaca tgctggagat cgaggactgg
     1741 gagggggaga cccttcgcac cctggcgagg gagatcgtcc ggctccctcc cttggaggtg
     1801 ccggaagggg cccgcagcat acgggacctg gggccggacg cccgcctggc gttacggcgg
     1861 ctggacgaga tattctggac cgagatgacc gacctgtcct cctaccagct ggtctcccag
     1921 gtgagcccag tgggaaggga cgagaaggcc acctcggtcg ttgagatatg caaacggatg
     1981 agcgtcccct tggaggactg catgtacata ggcgatggcg acaccgacgc ccgggccttg
     2041 caggtcgtcc gacgcagcgg agggctggcc gtggccttca acggcaacct gagcgcgctg
     2101 caggaggcgg aggtggggac gatcaccccg aacgccatcg tgacctcgat cctggccgaa
     2161 ctcttctaca ggggcggccg ggacggggtg ctggaggcga tcgaggggtg gagcacggag
     2221 ggtctgcggt ccacaggcat ggtgcataat tacctgctta gggaactctc ccgcaccttc
     2281 ccggaggcgc tccccacggt caggaggatg agcaaggaat gccttccggc catgttccac
     2341 gaggccgccc gaatgcgtat ccagatgcgc cgcccccttc ccaacgcccc ttccgacgaa
     2401 atgtcataaa ggggacgata cgttaaaata tgctctgcat tgtccttgac accatgacct
     2461 tctttctggt ccggatgctc cggcatcatt cccgcgcaga ccggtcgatg gtccctgacc
     2521 cggagatgct ggactattac ttcgaggagt atcagctggg gggcatcacc cgcaccctga
     2581 tccgttccga ccccccttcg aagggcgtca gccagtggct gccgctgggc acggagggcc
     2641 ataaggggca ggacgggacg gcgggtccag tggacgtctg gcttgtacac gacctcctca
     2701 acgacctcat gagctgacct ggaccgggca tttgatcagg catagccccc ataccctttc
     2761 atgtactgct ccgttctggt caccgccccc gacctcagga ccgccaggga catagggcgg
     2821 accatggtgg aaagggggtt ggcggcctgc gtgaacatat gggaggtcag ctccatctac
     2881 agatggaagg gcgaggtgca cgagggaccg gaggcggcga tgctgttcaa ggtccctagg
     2941 gaggggttcg acgccctgcg ctcggccatc ctcgaagtcc atccgtacga ggtgccgtgc
     3001 atagtgggct acgccatcac ggccggccat cccccctatc tggattggat ctccgagagc
     3061 gctcctccct ccggcggtga ggctcaaaag cctagaagga ctggcaacga tttaataggg
     3121 cgctgatccg ttcgctatcc gatcatgaac gatgagctag accagagccg cccgctgctc
     3181 ctggaccttc tgcgaaccct ccagcagtgc gaggacaggg aatcagccat cagggcggtg
     3241 atggacacgg tggcgggcat gctccgtccc gaaaggctgt gctatcttcc gaacaagggc
     3301 acgcccatcg gggacgccgg cgcatggacc tccggcgccc gccttcttcc cgaccagatc
     3361 gtgaagctcc tcccctccaa gaaggggttc tccctggcca tcaaggagaa ggggttgcgc
     3421 atcgggaccc tgtgcgctga cgacgtggag aggccgcgct ccttggacag catgctcccc
     3481 atcctgctca tggtcccgga cgccctggag atcgcgttct cgaacatcag ggcgctcgag
     3541 gggcttcgcc tctccgaggc taagctggca caactgtcgg agtccttggc ggtcgccaac
     3601 aggatcctta ggcacgacat tgcgaacgag atcctcgtgg taagttcgtc cctggacctc
     3661 tacaaggcca accaaaggga gagggacctc gcccgggcgc aatcgtccct gcagcgcatg
     3721 caaggcatcg tcctcaagat gagggagctg gacggcttcc tgctctcccg gacggagatg
     3781 atgccgacca acctgcgcga ggtcatcgac aaggcgatgc cccctgtgga gatcgcgtac
     3841 gaggtggtgg gggacgggaa cgtgctggcc gacccggcca tcggggcgat catcgagaac
     3901 atggcctcga acgccaagcg gcacggtatg gccgggcata tcaccttcac gatatcccaa
     3961 ggcaacggga cggtgctctt gaccgtgagg gacgacggga aggggatccc ggaggaacac
     4021 ctccccaggg tgttcatcga gggggcggcc ttcggggagg cgcgcgggac cgggctcggc
     4081 ctctacctca tcaaaaggac aatggagcgc tatggcggca gcatcaccgc cggcaacgac
     4141 ccccgcggag gggccaggtt cgaactcacc ttccagtccg cctaggcccc gggaagggga
     4201 tggaagggtc caatggcgaa gcgtccgccg ggccatcgcc ggccgcggag ccccggtcct
     4261 caccgtctcc cgtgcacgac ctcgttgatc cacaccacca ggcggcggta cgcctccctt
     4321 cccccgtcgg tgaggaccac cttctggtgc ccgtcctcct ctatcagggc ggtcaacccc
     4381 cgttcggtca tccagtcaag gtacctgctg aagacgtcat agttcgtgcc gaccgcgacc
     4441 tgcaggcggg tcctgaggat gggggaaggc tcccgccaca gcctctccag gaccctggcg
     4501 acgacataga ggtccggccg ctccatcagc tcagcgcccc ccgcccggtt ccagcaggag
     4561 gtagagctcg gccagcttgc ggtcccaatc ctcggtcgat cgcctgtgct ccttcctggc
     4621 cctaacgctc tcgtcgagga agaggcccgt tccaagcgcg atcagccctg ccccgatgat
     4681 ataggcgccc caccacccag ggccgtagag ggccatgatc tggcctcctg atatgaatag
     4741 gaggacgatg agaagggtct tccccggtgc ccggttccaa acccccctgc gccggacctc
     4801 gagccatctg ggcacctctc ggcagatgtc cagcaggagc cctaccgatg agctgacgcc
     4861 ctccctgtcc ctcatggcgg ccaatatcct ctccgcccag gcctcctccc tggcgatgtc
     4921 ctcctggcac cttctccgga gcaggaggac gcccccggct gtggcgccag ctatgatcag
     4981 cgtgccgatg ccgagcagca gggcatgccc atggccgctc gtcgggccgg tgagggccaa
     5041 gagcaccacc caggcgacca gggccaggga cacgaggacc agtccccggt cctgatacga
     5101 tcccttctcc acgtccaacg catcgcgggc cagaatgtac gcgctggaca ggctgtccac
     5161 cataagctcc tccacccccc tgtcctccgc cgggccgacg cccctcatct tggaggagag
     5221 ggcctccacg ccctcccaac attgggcgta tatataaagg gagaaaagga acaa
//
LOCUS       DAOSYN010000087         5263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_13130_length_5263_cov_4.402266, whole genome shotgun sequence.
ACCESSION   DAOSYN010000087 DAOSYN010000000
VERSION     DAOSYN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13130_length_5263_cov_4.402266"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..515
                     /locus_tag="PLJ11_04685"
     CDS             36..515
                     /locus_tag="PLJ11_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04001.1"
                     /translation="MRFEHDRRSLTINLALWAIAAVWLMALLALLPGVRLLHLLLVGS
                     VLTIAVLVVGVSPLLTPHEVTEGRIILRQGWHHRLVVPVARVKRLERLDRIEAKEGVL
                     MDAFNRTLVMTDGKANGLRLELKEAVRVPSALWKKVDAVIFDVDEPDRFIAEVDKAR"
     gene            complement(516..1313)
                     /locus_tag="PLJ11_04690"
     CDS             complement(516..1313)
                     /locus_tag="PLJ11_04690"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOO04002.1"
                     /translation="MPQLKELTVETITDFIQTQVREAGASGVVIGLSGGIDSAVVTKL
                     CVDALGGDKVLNVFMPSPSTPTQDLRDVQGMSRAWGTTLLVYDIEQLLRTYHKIMPGS
                     QSKEVSGNLIARIRMTILYAHANMEGRLVMGTGNKSELLMGYFTKYGDGGADLMPIGD
                     LYKTEVRELARVIGVPEGIIAKEPSAGLWEGQTDESEMGIAYDDLDRVLSGLEDQLPL
                     DVISKRSGVPMELVRMVATKNATSVHKRRLALIPKLGGRTIGCDWRE"
     gene            1683..2465
                     /locus_tag="PLJ11_04695"
     CDS             1683..2465
                     /locus_tag="PLJ11_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOO04003.1"
                     /translation="MRVALCQLRSAVGDVQANLNRVREVMEGTDADLFVFPETYLTGY
                     MVRDRVVALAEDMDGESINHLLELADRTGKAVVVGVPLNDPCNHGLITNSAVMVTPDG
                     RVQRYDKLHPANFGPFEEGLYFTAGQTPAMFQLRGHGIGVCICYDLFFPEICKAYAMQ
                     GADMLLCIAASPVTSRTPFETMVPARAVENTCPIAYVNNVGSQQNMVFFGGSQAYDAK
                     GRPLAKLPYLREAVEVVDVDLLQNGPARRARPTLRDSLGSLR"
     gene            2519..2619
                     /locus_tag="PLJ11_04700"
     tRNA            join(2519..2556,2571..2619)
                     /locus_tag="PLJ11_04700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2553..2555,aa:Ser,seq:gga)
     gene            2734..3231
                     /locus_tag="PLJ11_04705"
     CDS             2734..3231
                     /locus_tag="PLJ11_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04004.1"
                     /translation="MRVNVDLRLKDVPGQLIKALEPISLNEGNIVGVVHNHDLVTGGR
                     ISVNVTFEYKDQRALDAISSAWKERGVEVVKMGEIFRTYAAEYLLVGDLTHAILRTIT
                     ESIERMDGVETMDLQYSLATGTADRSVLLVVSVRERERVRDIDSYLGAMSERHDFLLI
                     RGMGV"
     gene            3233..4255
                     /locus_tag="PLJ11_04710"
     CDS             3233..4255
                     /locus_tag="PLJ11_04710"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOO04005.1"
                     /translation="MRLALVGFGTVGQGLAEAVAMKKDLIYDRFGTELRIVAAFDSRT
                     YAMDPRGLKPLELVARKQAGKGLGRIRPKSVAEMLRELDYDVLVEMTPTNIEDAEPGY
                     TNIRTALECCRDVVTSNKGPLALRFRTLDELARKNGVELRFEATVGGATPIINLSREL
                     LMAEKVESMKGILNGTCNFILSRMGEEGLPFEYVLKEAQEMGYAEADPSYDIDGIDSA
                     SKLAILANAIMGMDVTYHDVRRTGIRRITQEAVELAAKERKVIRLIGEVNGEGVEVAP
                     RMVPLGHPLAISGTLNVLHMHTDLAGAITVVGRGAGRKETASAIMSDLVGLLKRNACL
                     LNGRVK"
     gene            4366..>5263
                     /locus_tag="PLJ11_04715"
     CDS             4366..>5263
                     /locus_tag="PLJ11_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOO04006.1"
                     /translation="MRDGAQTEGVCFSNEDKLEILERLDAFGIDFIEGGYPASNPKDR
                     AFFQAARRVELRNSKLVAFGATHRSGVRPEEDKGMDALLEAGTEWVCVFGKAWDLHVE
                     KALQVPLEENLRIVRDTVAYLKGKGRSVIFDAEHFFDGYQHNAEYAMGVLHEAEDAGA
                     DWLCLCDTNGGAFPSLVGKVVSRVREEMRAPLGIHAHNDGELAVGNSLAAVEAGAAMV
                     QGTVNGLGERCGNANLCSIIPNLMLKMGVRTGISDLSQLTKLSHFVAEMANVAPDPRA
                     PFVGKSAFAHKGGIHVSAVLKDP"
BASE COUNT          981 a         1565 c         1796 g          921 t
ORIGIN      
        1 gggatatatc catacaatcc cacgtacacc tttgaatgcg gttcgagcac gatcgccgtt
       61 ccctcacgat caatttggca ttgtgggcca tcgccgcggt ctggctgatg gcgctcctag
      121 ccttgcttcc gggggtgcgc ctccttcacc tgctgttggt cggttccgtc ctcacgatcg
      181 cggtgctcgt cgtcggcgtc tccccgctgc tcacccccca cgaggtgacc gaggggcgca
      241 tcatactgag acagggatgg caccaccgcc tggtggtgcc cgtggcccgc gtcaagcggc
      301 tggagcggct ggaccgcata gaggcgaagg agggggtgct tatggacgcc ttcaaccgca
      361 ccttggtcat gaccgacggc aaggcgaacg ggctgcggtt ggagctcaag gaggcggtgc
      421 gggtaccatc ggccctctgg aagaaggtcg acgccgtgat cttcgacgtt gacgaaccgg
      481 accgcttcat cgccgaggtc gacaaggccc gatgatcact cgcgccagtc gcagcctatg
      541 gtgcgccccc cgagcttggg gatcagggcg aggcgcctct tgtggaccga tgtggcgttc
      601 ttggtcgcga ccatcctgac cagctccatg ggcaccccgg acctcttgga gatgacgtcc
      661 agggggagtt ggtcctccag cccggagagg acgcggtcga ggtcgtcgta ggctatgccc
      721 atctccgact cgtccgtctg gccctcccag aggcccgcgg aaggctcctt ggcgattatg
      781 ccctccggaa cgccgatcac cctggccagc tccctcacct cggtcttgta caggtcgccg
      841 atgggcatga ggtccgcccc tccatccccg tacttggtga aataccccat gagcagctcg
      901 gacttgttgc ccgtgcccat gaccaatcgc ccttccatgt tggcatgggc gtacaggatg
      961 gtcatacgga tgcgggcgat gaggttcccg gacacctcct tgctctgcga acccggcatg
     1021 atcttgtggt atgtcctcag cagctgctcg atgtcgtaca ccaagagggt cgtcccccag
     1081 gcccggctca tcccctgcac gtcccgaagg tcctgcgtgg gggtgctcgg tgagggcatg
     1141 aacacgttca gcaccttgtc cccgcccagg gcgtccacgc acagcttggt caccacggcc
     1201 gagtcgatgc ccccggacag gccgatcact acccccgagg ccccggcctc cctgacctgc
     1261 gtctggatga agtcggtgat cgtctccacg gtaagctctt tcagttgcgg catcggatac
     1321 ggtaatgccc tccagtttaa taattctctt tccggggacg tgctgaacga gctggagaag
     1381 agggcgttcg tttttccata tcaatgatga cgccgtcctt ggggcatcta cgttgaccag
     1441 aggaaccggg tccgggcgga cggctctcgt tgaaatgcca gggttcgggg gagggcagga
     1501 cggtgcgcta cgcgatggag tatggggtga taggctggaa acggtcctcc tcgcccctga
     1561 tgaagggaga actcaaccgg gacatggccc tcaggggtgc cactaggagc tgttcctcgc
     1621 gatccctctc cggcaaacca ttcggcaaag gttgattacc gccgttggcg ttggggcaca
     1681 ccatgcgggt tgccctttgc cagctccgga gcgccgtggg ggacgtacaa gccaatctga
     1741 acagggtcag ggaggtgatg gagggcacgg acgccgacct gttcgtgttc ccggagacct
     1801 atctcacagg gtacatggtc agggaccgcg tggtcgccct agccgaggac atggacgggg
     1861 agagcatcaa ccacctgctg gagctggccg accgtacggg caaggccgtg gtcgtcgggg
     1921 tcccgttgaa cgacccctgc aatcatggcc tgatcaccaa cagcgcggtg atggtgaccc
     1981 ccgacggaag ggtgcagagg tacgacaagc tacacccggc caacttcggg ccgttcgaag
     2041 agggactgta cttcaccgcc ggccaaacgc ccgcgatgtt ccagctcagg gggcatggga
     2101 tcggtgtctg catctgctac gacctgttct tcccggagat atgcaaggcc tatgccatgc
     2161 agggggcgga catgctgctg tgcatcgcgg cctccccggt cacctccagg acgcccttcg
     2221 agaccatggt tccggcgagg gcggtggaga acacctgtcc aatcgcctac gtgaacaatg
     2281 tcggaagcca gcagaacatg gtgttcttcg gcgggagcca ggcgtatgat gcgaaggggc
     2341 ggccgctggc caagctgccc tatctgcggg aggcggtcga ggtggtggac gtggacctgc
     2401 tgcagaacgg cccggccagg agggctaggc ccaccctgag ggattcgctg gggagcctcc
     2461 ggtgaaccgc cgagcgcaaa tagttttaaa tgagcgcgcg gttatcgccg ctggaagtgc
     2521 cgaggtggcc cagaccggta aggcgcgagc ctggaaactt tccgccagct agctcgtggc
     2581 ggcaacgcct cgggagttca aatctccccc tcggcgccat ctccccgctc ccgtccctga
     2641 cgctggggat tgaaagcatt acataatgga atccttatat cgggccgggc accgcggacc
     2701 ggcatcctgc ccaaggtgcg aggggtcatc cagatgaggg tcaacgtgga tctgcgcttg
     2761 aaggacgtcc caggacagct catcaaggcc ctggagccga tatcgctcaa cgaggggaac
     2821 atcgtgggcg tggtgcacaa ccatgacctg gtgaccgggg ggcgcatatc ggtgaacgtg
     2881 accttcgagt acaaggacca gcgcgccctt gatgcgatct cctcggcctg gaaggagcgg
     2941 ggggtggagg tcgtgaagat gggggagatc ttccgcacct acgccgccga gtacctcctg
     3001 gtcggggacc tgacgcatgc catcctgagg accatcaccg agagcatcga acggatggac
     3061 ggggtggaga ccatggacct gcagtactcc ctggccaccg gcaccgccga caggtccgtg
     3121 ctgctggtgg tgagcgttcg ggaaagggag agggtgaggg acatcgattc ctacctgggg
     3181 gccatgagcg agcgccacga cttcctcctg atccgcggga tgggggtgtg acatgcgcct
     3241 ggcgctcgtg ggcttcggga ccgtcggcca gggattggcg gaggcggtgg cgatgaagaa
     3301 ggacctcatc tacgaccgct tcggcaccga actcaggatc gtggcggcct tcgactcgcg
     3361 cacctatgcg atggatccca gggggctcaa gccattggag ctggtggctc gaaagcaggc
     3421 cgggaagggg ctgggaagga tcaggccgaa gagcgtggcg gagatgctca gggagctgga
     3481 ctacgacgtc ctggtggaga tgacccccac gaacatcgag gacgccgaac cggggtatac
     3541 gaacatccgc accgccctcg aatgctgccg ggacgtggtc acctccaaca agggcccgtt
     3601 ggccttacgg ttccgaacgc ttgacgagct tgccaggaag aacggcgtgg agctgcgctt
     3661 cgaggccacc gtgggcgggg cgacgccgat aatcaacctc tcccgggagc tcctcatggc
     3721 cgagaaggtc gaatccatga aggggatcct caacggcacg tgcaacttca tcctcagccg
     3781 gatgggggag gaggggcttc cgttcgagta cgtcctgaag gaagcgcagg agatgggata
     3841 cgcggaggcg gaccccagtt acgatatcga cggaatagac agcgccagca agctggccat
     3901 ccttgccaac gccatcatgg gcatggacgt cacctaccac gatgtgcgcc ggaccggcat
     3961 ccgccggatc acccaggagg cggtggagct ggcggccaag gagaggaagg tgatcaggtt
     4021 gataggcgag gtgaacggcg agggggtcga ggtggccccg cggatggtgc ccctagggca
     4081 ccccctggcc atatcaggga cgctcaacgt gcttcatatg cacaccgacc tggccggggc
     4141 gataacggtg gtggggcggg gggccggcag aaaggagacg gccagcgcca tcatgagcga
     4201 tctggtgggg ctcctgaagc ggaacgcctg cctgttgaac ggtagggtta aataggtcgt
     4261 gccatgatag agcatacctt acctgagggg tcgcaatggc aaggacgtcg aactacgggt
     4321 gggatctatt tattgcccga cagggctagg ctattcgata ccacattgag ggacggggcg
     4381 cagacggagg gcgtctgctt ctcgaacgag gacaagctgg agatcctgga gcgattggac
     4441 gccttcggca tcgatttcat cgagggcggc tacccggctt ccaaccctaa ggaccgggcg
     4501 ttcttccagg ccgcccggag ggtcgagctt aggaacagca agctggtggc cttcggcgcc
     4561 acccaccgct ccggggtccg gccggaggag gacaagggca tggatgccct gctggaggcc
     4621 gggaccgaat gggtgtgcgt cttcggcaag gcctgggacc tgcatgtgga gaaggcgctg
     4681 caggtgccgc tcgaggagaa cctgcggata gtgcgggaca ccgttgcgta cctgaagggg
     4741 aaggggcgca gcgtgatctt cgacgccgag cacttcttcg acggctatca acacaatgcg
     4801 gagtacgcga tgggcgtatt gcacgaggcg gaggacgcgg gggcggattg gctgtgcctc
     4861 tgcgacacca acggtggggc cttcccgagc ctggtcggga aggtcgtctc ccgggtcagg
     4921 gaggagatgc gggcgccgct cggcatccac gctcacaacg atggggagct ggcggtcggc
     4981 aattccctgg cggcggtgga ggccggggcg gccatggtcc agggcacggt caacggcctg
     5041 ggggagcgct gcggcaacgc caacctgtgc tcgatcatcc ccaatctcat gctcaagatg
     5101 ggcgtgagga ccggaatctc agatctgagc cagctgacaa agctttccca tttcgtggcc
     5161 gagatggcca atgtcgcgcc cgatccccgc gccccgttcg tgggcaagag cgccttcgcc
     5221 cacaaggggg ggatccacgt aagcgcggtg ctcaaagacc ccc
//
LOCUS       DAOSYN010000088         5185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_13376_length_5185_cov_4.442885, whole genome shotgun sequence.
ACCESSION   DAOSYN010000088 DAOSYN010000000
VERSION     DAOSYN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13376_length_5185_cov_4.442885"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLJ11_04720"
     CDS             complement(<1..427)
                     /locus_tag="PLJ11_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04007.1"
                     /translation="MDWTMMLAAALGIGPALALMFWTLQDYTYPRVERPFFDDRKLFL
                     MLAVGMVIGVIVYVLQSYFDLGYLLFALLFAVVEELLKLVILNFPRFARRLDTSFYGL
                     SMGLGIGSTMAFGAVLSTIQQSDGLGAMAWVMVIILGVQL"
     gene            complement(454..2373)
                     /locus_tag="PLJ11_04725"
     CDS             complement(454..2373)
                     /locus_tag="PLJ11_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOO04008.1"
                     /translation="MSNYQHGKVRFRWCDTCGTLVLGEVCGICGRPGREFEVSAPGDI
                     RPCFGKGVDTVAELFQRHFGTSGLLRTRNVFLNKVAGEDRADEVVLDGRVVAVIRFEL
                     VRDDFVLELKAEGAALLAKAARKGVIRARRPPGHLKGKKLDGADVLEVLGDLKAGDPV
                     VVVMGNTVAAGEARVGSDHMKEGEKVVHLRDVCKADVPFPLVPADWNDFVKANQKHLR
                     QLEAAAISDLRSFAHNSALPISLSFSGGKDSLACYGLTKKALGRFDLLFIDTGLEFPE
                     TVQYVRSFARSRGERLRVAKAGNAFWENVGAFGPPAKDFRWCCKVCKLGPLAALIEEN
                     YRQGTVTVEGNRALESFARSRIGFVERNPFVPNQTVLNPIRDWRAAEVWGYIWMEGLE
                     YNPLYEQDLERIGCYLCASCLGSEWRAVRQLHPDLYSEWERWLGEWGERVGVSPDFVR
                     YGFWRWKALPPKMRLVADELHMKVPQMRNDSMELRISKGASPCVAGGFSMEGVLTIPR
                     KRDFSQVAEALKTVGKVKFSPEFEVVMVKTKGGTLKMFGGGHISAIAPTKEEAERLFR
                     AGAEAFLRGQLCTSCRICERNCRFKAITADGQLKVDERLCQQCGECAKACVVAHYYDK
                     LVQAGDGKPVYAGVK"
     gene            2450..3124
                     /locus_tag="PLJ11_04730"
     CDS             2450..3124
                     /locus_tag="PLJ11_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent protease"
                     /protein_id="HOO04009.1"
                     /translation="MRSASEMYDEYGNLVIPRGYGRIHFSRQEVVQILISVAVLTIAF
                     TVVLLGGLFGDLESLDTVVWLFALGVSAVVSITGFLLHELAHKFVAQRYGAWAEFRMY
                     PLGLLMALLFSFLGFLLAAPGAVFISGRINRKQNGVISLAGPATNIAFGGAFVALWLV
                     FPDAGIWSTAFNLIGQLSLLLGVFNLLPFGPLDGRKVWSWSPATWVMAMAVAAALLMV
                     SWGLFI"
     gene            complement(3117..4556)
                     /locus_tag="PLJ11_04735"
     CDS             complement(3117..4556)
                     /locus_tag="PLJ11_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOO04010.1"
                     /translation="MTMDERLVREIAAIVGEGDCSTRAADLYTYGFDSSIYHHSPDVV
                     VRPRNAEEVSALMRFASREGIPVVPRGGGTGLCGGAVPIRGGMVMDLSRMDRIKEIRV
                     NDLYCVVEPGVVYDHLNDALAPKGFFFPPTPGSGEACTIGGMVATNASGMKAVKYGAT
                     RDYVLGLEVVLADGSLIHVGTNTIKNSSGYQLERLFVGSEGTLGIITEVTLKVAPRPL
                     RAAMVLAAFDSLESAGACVSNLISVPLIPSATELMDDVCIRAVNKAVNAGLPDAQAIC
                     MIEVDGGPETVARELKVAEDVAVRSGAFHVQVSDDGRQMAKWTSARKSVMSALSRLGD
                     DLVSVSLADDMAVPVSKVPEAVVAFHRIAEENRVIVGTYGHAADGNLHTKMLLDPCSE
                     DSWKRGERAVGQIYDTVIALGGTVTGEHGVGISKAPYMMRERPDAWRTMLAIKNALDP
                     QNILNPGKLMQWEGGIIRDLRYPCPGPIR"
     gene            4647..5009
                     /locus_tag="PLJ11_04740"
     CDS             4647..5009
                     /locus_tag="PLJ11_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOO04011.1"
                     /translation="MEQRSTTRAVKLSPERFLSLMAKVGKGECVLIDVRSHEEYRAER
                     MEGAVNFPVDSIVGSVGGMDRGTPLLVYCRTGNRSVRAAEQLARMGFNDISVLEGGIE
                     ALKAYLGRSAGASGPPVR"
BASE COUNT          888 a         1742 c         1578 g          977 t
ORIGIN      
        1 ccaattggac cccgagaatg atcaccatca cccaagccat cgcccccagt ccatcggact
       61 gctggatggt tgaaaggacc gcgccgaagg ccatggtgct ccctatgccc agccccatgg
      121 acagcccgta gaacgaggtg tccagcctgc gggcgaagcg ggggaagttc agtatgacaa
      181 gcttgagcag ctcctccacg accgcgaaga ggagggcgaa cagcaggtac ccgagatcaa
      241 agtaggattg gagcacgtaa actatgacgc cgatgaccat gcccaccgcc agcatcagga
      301 acagtttgcg gtcgtcgaag aagggcctct ccaccctggg gtacgtgtag tcctgaaggg
      361 tccagaacat caaggccagg gctggaccta tccccagggc tgcggccaac atcatcgtcc
      421 aatccatggt cgggtcgccc caccccggtc cgattattta acgcctgcgt acacaggctt
      481 cccatccccg gcctggacca gcttatcgta gtagtgggcg acgacgcatg ccttggcgca
      541 ttccccgcac tgctggcaca ggcgctcgtc caccttgagc tgcccatcgg cggtgatggc
      601 cttgaacctg caattgcgct cgcatatgcg gcaggaggtg cagagctgtc ctctcaggaa
      661 cgcctccgcc cctgcccgga acagcctttc cgcctcttcc ttggtcgggg ctatggcgga
      721 gatgtgcccc cctccgaaca tcttcagggt cccgcccttg gtcttgacca tcaccacctc
      781 gaactccggg gagaacttca ccttcccaac ggtcttgagg gcctccgcca cctgcgaaaa
      841 gtccctcttg cgggggatgg tcagtacccc ttccatcgag aacccgccgg ccacgcaggg
      901 gctggccccc ttggagatcc taagctccat gctgtcgttc ctcatctgcg gcaccttcat
      961 atgcagctca tcggccacca gcctcatctt gggcggcaac gccttccagc gccagaaccc
     1021 gtagcggacg aagtccgggg agaccccgac ccgttccccc cattccccca gccagcgctc
     1081 ccattcggaa tacaggtccg gatgaagctg ccgtaccgcc cgccattcgc tgcccaggca
     1141 ggatgcgcac aggtagcagc ctatgcgctc aaggtcctgc tcgtaaaggg gattgtactc
     1201 caggccctcc atccagatat acccccagac ctcggcggcc ctccaatccc ggatcgggtt
     1261 caggacggtc tggttgggga cgaacgggtt ccgttcgacg aacccgatcc tcgaacgcgc
     1321 gaaggactcc aaggcgcggt tcccctccac ggtgaccgtg ccttggcgat agttctcctc
     1381 gatcagggcc gccaggggcc ccagcttgca caccttgcag caccatcgga agtcctttgc
     1441 cggcgggccg aaggcgccta cgttctccca gaacgcgttc cccgccttgg cgaccctgag
     1501 cctctcccct ctggacctgg cgaaggagcg cacgtattga acggtctccg ggaactcgag
     1561 gccggtgtcg atgaacagca ggtcgaacct cccaagcgcc ttcttcgtca ggccgtagca
     1621 tgccagggag tccttccccc cggagaagga cagggagatc ggcagggcgc tgttgtgggc
     1681 gaaggagcgc aggtcgctga tggcggcggc ctccagctgg cggaggtgct tctggttggc
     1741 cttcacgaag tcgttccaat ccgccggcac cagggggaag ggcacgtccg ccttgcagac
     1801 gtcccgaagg tggacgacct tttccccttc cttcatatga tcggagccga cgcgggcctc
     1861 ccccgccgcg actgtattgc ccatgaccac gaccacgggg tcccccgcct tgaggtcgcc
     1921 gagcacctcc aggacatccg ccccgtccag cttcttcccc ttgagatggc ccggcggcct
     1981 cctggccctg atcaccccct tccgggcggc cttcgccagc agcgccgccc cctcggcctt
     2041 gagctccagc acgaaatcgt ccctcaccag ctcgaagcgg atgaccgcca ccacccgccc
     2101 gtccagcacc acctcgtcgg cgcggtcctc ccccgccacc ttgttcagga agacgttcct
     2161 ggtgcggagg aggcccgagg tcccgaagtg cctctggaag agctcggcca cggtgtccac
     2221 gcccttgccg aagcagggac ggatgtcccc gggggcggat acctcgaact cccttcctgg
     2281 acggccgcag atcccgcaga cctccccgag gaccagggtg ccgcatgtat cgcaccaccg
     2341 gaagcgtacc ttgccgtgct ggtagttgct catttcccca agctatccct cgccgatgta
     2401 cttcaagctt gtcctacgcc ggggacgggc aatatatatc tagtaggtga tgcgctcggc
     2461 gtccgagatg tatgatgaat acgggaacct ggtcattccc cgcggctatg ggcgcatcca
     2521 tttcagccgg caagaggtcg ttcagatcct gataagcgtg gcggtgctca ccatcgcctt
     2581 cactgtcgtc ctgctcgggg ggctctttgg agacctggaa agcctggaca cggtggtgtg
     2641 gctcttcgcc ctgggcgtat cggcggtggt atcgatcacc ggcttcctgc tgcatgagct
     2701 ggcgcacaag ttcgtcgccc agcgctatgg tgcctgggcg gagttccgca tgtatcccct
     2761 ggggctgctc atggccctgc tcttcagctt ccttggtttc ctcctggccg cccccggggc
     2821 cgttttcatc tccggccgga tcaacaggaa gcagaacggc gtgatctccc tggccggtcc
     2881 ggccaccaac atcgccttcg gaggggcgtt cgtggccctg tggctcgtgt tcccggacgc
     2941 gggcatctgg agcaccgcct tcaacctgat cggtcagctg tcgcttctgt tgggggtgtt
     3001 caacctactc cccttcggtc ccctggacgg caggaaggtg tggtcctgga gcccggccac
     3061 ctgggtgatg gcgatggcgg tcgcggcggc cctgctgatg gtaagttggg gcctcttcat
     3121 ctgatcgggc cggggcacgg gtagcgaagg tcccggatga tccctccctc ccactgcatg
     3181 agcttgccag ggttcagtat gttctggggg tccagggcat tcttgatggc caacatggtg
     3241 cgccacgcgt ccgggcgctc ccgcatcata tagggcgcct tggagatgcc gacgccgtgc
     3301 tcccccgtga ccgtgcctcc caacgctatc accgtgtcgt agatctgccc gaccgccctc
     3361 tcccccctct tccaggagtc ctccgagcag ggatcgagca gcatcttggt gtgcaggttg
     3421 ccatcggccg cgtggccgta ggtgcccacg atcacccggt tctcctctgc gatgcgatgg
     3481 aacgccacca cggcctccgg gaccttggac acggggacgg ccatgtcatc cgccagggac
     3541 acggagacga gatcgtcgcc gaggcggctc agggcggaca tgaccgactt gcgagcgctg
     3601 gtccacttgg ccatctgcct accgtcgtcc gagacctgga catggaaggc cccgctccgg
     3661 accgcgacgt cctccgccac cttcagctcc ctggccaccg tctccggccc tccgtccacc
     3721 tcgatcatac agatggcctg ggcgtccgga aggccggcgt tcaccgcctt gttcaccgcc
     3781 ctgatgcaga cgtcgtccat cagttcggtg gccgagggga tgagggggac ggagatgagg
     3841 ttggagacgc aggccccggc gctctccagg ctatcgaacg cggccagcac catggccgcc
     3901 ctcaggggcc tgggggcgac cttgagggtc acctcggtta taatgcccaa tgtcccctcg
     3961 ctccccacga acaggcgctc caactgataa ccggacgagt tcttgatggt gttggtcccg
     4021 acatggatga gggagccgtc ggcgaggacg acctccaggc cgagcacgta atcgcgcgtg
     4081 gcgccgtact tgaccgcctt catcccggag gcgttcgtgg ccaccattcc gccgatggtg
     4141 cacgcctccc cgcttcccgg ggtgggaggg aagaagaacc ctttgggggc gagcgcgtcg
     4201 ttcagatggt cgtacacgac cccgggctcg accacgcagt agaggtcgtt cacccgtatc
     4261 tccttgatgc ggtccatccg ggagaggtcc atgaccatcc cgcccctgat gggcacggcc
     4321 cctccgcata gccccgtccc tccgccccgg gggacgaccg gaatcccctc cctggaagca
     4381 aatcgcatca atgcggagac ctcctcggcg ttcctgggcc ggaccaccac atccgggctg
     4441 tgatgatata tggaggagtc gaaaccgtat gtgtagaggt ccgcggcccg ggtggagcag
     4501 tccccctcgc ccactattgc ggcgatctcc ctcaccaggc gttcgtccat cgtcatggtg
     4561 ggcgagatac gcggccgggg atagataatc gttgcccatg ccctagaacg atatgctaat
     4621 gtactattag ctgaggatag gggacgatgg agcaacggtc gacgacccgg gcggtcaaac
     4681 tgtccccgga gcggttcctg tcgctgatgg ccaaggtcgg gaagggcgaa tgcgtcctga
     4741 tcgacgtccg cagtcacgag gagtaccggg cggagaggat ggagggggcc gtgaacttcc
     4801 ctgtggattc gatcgtcggc tccgttgggg gaatggaccg tggaacgccc ctgctcgtct
     4861 attgcaggac cggcaacagg tccgtcaggg ccgcggagca attggcccgg atggggttca
     4921 acgacatctc ggtcctggag ggagggatcg aggccctgaa ggcgtacctc ggaagatcgg
     4981 cgggggcgtc cggcccgccg gtacggtagg gcggcctctc aacggtattt aatgattgct
     5041 ctttccgcgc ctagacatcc catcctgctc gatccctgtt gatgctggcc ggtacgagat
     5101 ccgtagaggc cgattggatg gatgagccat ccataacaat ggtttataat ccctaatgac
     5161 cgtgtgtttt ttacaccaat ggaat
//
LOCUS       DAOSYN010000089         5152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_13467_length_5152_cov_4.776143, whole genome shotgun sequence.
ACCESSION   DAOSYN010000089 DAOSYN010000000
VERSION     DAOSYN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13467_length_5152_cov_4.776143"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /gene="serS"
                     /locus_tag="PLJ11_04745"
     CDS             <1..1097
                     /gene="serS"
                     /locus_tag="PLJ11_04745"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOO04012.1"
                     /translation="LRISKLSGEEKAEAVTRMKEVAVRIGQLDARVAELEAQRDEVVL
                     LFPNIPHETVPIGDCYEKNRIVMEFGKVRDFDFQPKDHIALGEDLDIIDFQRGVKVAG
                     SGFFVLKGDGARMERALINYMLDLHHRQGYTEVFPPVVVNRAAVIGTGQYPKMKDDMY
                     WCERDDLWLNPTAEVPVTNLLQDEILAKEDLPIYYTAYLPSFRREAGRHAEMKGMIRV
                     HEFNKVELVKFVLPDGSFQELESLLNDAQDVLRGLELPFRTLLLCTGDMGFASAKTYD
                     LEAYAPGSGSWLEVSSCSCFTDFQARRARIKYRPEPHLKSEFVHTLNGSGLALPRTMV
                     SIMENCQDREGRIHIPKVLRPYMQGQELIE"
     gene            complement(1137..1403)
                     /locus_tag="PLJ11_04750"
     CDS             complement(1137..1403)
                     /locus_tag="PLJ11_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04013.1"
                     /translation="MVSLNDLIEELDCTLEDFLDQVTLAIDLLEEGEQEEAVEVLKGL
                     EEDLFDFLGYEEMEEGEEEAEEEEEEEEEEEDKPKKAPAKKGKK"
     gene            complement(1502..>5152)
                     /locus_tag="PLJ11_04755"
     CDS             complement(1502..>5152)
                     /locus_tag="PLJ11_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOO04014.1"
                     /translation="DYPLKGLGQVPWPLLKDRMDSFEREVNDGLGDLSSMVNYMLESL
                     AAYRTLQGWGASVNGARGLEETLTERDLLNALDLGLVLLQMEVFRSASPCLGMRTDAL
                     DGQGCWDYVSAALSGGGRIDPADLFLGLYGYDELDWRRVFSQALYSAVDKIALRWMEH
                     LHLIDLLEIVETVGEKIYFAVNDLIDDVLDVNMAERRFKEWLEEEFEASGVPEQLYRY
                     LGSDGPEETIQVRPGTMLLLDEDWEEVPALIGGDVVLDIPKVDIMAWEGWGDFHEQYK
                     KGTKEIISTIRSEIYAVAEQISMGLFLPSTILSLDPRDGKPFLEEIKASLSDALCNKE
                     RWLRPAIAAAEVSVTSMDPMAEAAKAELRENRDDILRRQEVIDTLVSSVAEQLLSTAM
                     ASMPDASLLWDENLRAIEGRIIEDGPWGVYAAMEDAFERRAEFLEEQFVRGLSAGSGD
                     GRSSCLAEVIARTGDPTAGLGVVLTDDVNDLLSEISHGLSLRGNQVEVDIPATPGFAL
                     LGTDGRVYHESLQVQVSYPSMNGPGERLVVSIADPRHFQGGQGEPQTHDTDLVSMKTA
                     SFQSTWGISFDGEVDVTVLSGGEAGEVVPMELRRGIALGSEQTVTVLTAHALMGVDYV
                     NINTLGDHLSNVIEGLLRPIREGLEDISSGLRAVYRLIQSTIDRLIDTGKEVLEILSA
                     KLGALVEQLQDFLRKAISTIEEKLAEVLLDIIGERSFRLCFLGVEMVIDVRPRDMMYR
                     EVSIPASFSMAFRANGCRIDVTTRLIKGPEEMSLLANASLLGDGWSVHAVIDPFMDVF
                     QHMVEVRGIMDDACVELVMPEVLCYREVTFALSDIPGVGALLANIPLPIPGLKGSVDA
                     GISLMVLEGRTDDVVINEYELNPAGEDAGREWVELYNPTSQVVDLAGWSLETRHGVQG
                     ALPLSGVLLPGSRTVHHFPGQALDNMGEGFPYEEGIVLRDEHGRRVDSTPFSTDCWND
                     GRTWQRAKDGVDRWEFRDGTKGTSNGKDHLSRFDLDPLQQAFAAAVTESIYQLTSTGP
                     SMEGLALTLETAILRFGERLSSEALEKEVVIGAYVEVAVTEASSTAKVGMRMEAVWRC
                     GTLRDTLHDLVGSAGVVVASFGNPFRAYLDGLSGADGIWLGMSSFGAIGIPKMISAPG
                     LDLEVRYQTTVSVNLGVLAMALGVRSPGWAVEAGAKVSGVPAAAVPALKVPVGTMADI
                     WLCKATVQRCMV"
BASE COUNT          985 a         1660 c         1470 g         1037 t
ORIGIN      
        1 ccctgcgcat atcgaagctg agtggggaag aaaaggccga ggcggtgacg aggatgaagg
       61 aggtcgctgt tcgcattgga cagctggatg ctagggtcgc cgagctggag gcccagaggg
      121 atgaagtggt gctccttttc ccgaacattc cgcacgagac ggtaccgatc ggggactgct
      181 atgagaagaa ccgcatcgtc atggagttcg gcaaggtgcg cgatttcgac ttccagccta
      241 aggaccacat cgccctgggg gaggacctgg acatcatcga cttccagcgt ggggtgaagg
      301 tggccgggag cggcttcttc gtgctcaagg gcgacggtgc caggatggag cgggccctca
      361 tcaactacat gctcgacctg catcatcgcc agggctatac ggaggtcttc ccgcctgtgg
      421 tcgtcaaccg agcggcggtc atcggtacgg gccagtaccc caagatgaag gacgacatgt
      481 actggtgcga acgggacgac ctttggttga accctacggc ggaggttccg gtcaccaatc
      541 tgctgcagga tgagatattg gccaaggagg acctgcccat ctactatacc gcctacctgc
      601 cctcgttccg aagggaggcg ggccggcatg cggagatgaa ggggatgatc cgggtgcatg
      661 agttcaacaa ggtggagctg gtgaagttcg tgcttccaga tggctcattc caggagttgg
      721 agtcgttgct caatgacgct caggacgtgc tgcgcggtct ggagctgccc ttccgcacgc
      781 tcctgctatg cacgggggac atggggttcg cttccgcgaa gacctatgac ctggaggcct
      841 atgcgccggg atctggaagc tggcttgagg tgtcctcatg cagctgcttc accgatttcc
      901 aggcccgccg ggcaaggatc aagtaccgtc ccgagccgca cctcaagagc gagttcgtgc
      961 acacactgaa cggctccggg cttgccctgc caaggaccat ggtgtcgatc atggagaact
     1021 gccaggatcg cgaagggcgc atacacatcc ccaaggtcct gcggccttac atgcaagggc
     1081 aagagctgat agaataggag agttgaaacg gtttctgggc ctccggatgg ccccttctac
     1141 ttcttacctt tcttggccgg ggctttcttg ggcttatcct cttcctcctc ttcctcttcc
     1201 tcctcttcct ccgcctcttc ctcgccctct tccatctcct catatccaag gaagtcgaag
     1261 aggtcctctt cgagcccttt caatacctca acggcctctt cctgctcgcc ttcctcaaga
     1321 aggtcgatgg ccagagttac ctggtctaaa aagtcctcca gggtgcagtc gagctcctcg
     1381 atcagatcgt tcaggctaac catatgctgt acctcggcct ccaatatcct ttgccaatag
     1441 ataagatatg gggtgcctcc aagcggccta gccccgatgc cgatgaatgc aattgaacgg
     1501 cctagaccat gcacctctgc acagtggcct tgcatagcca gatgtctgcc atggtcccca
     1561 ctggtacctt gagggcgggc acggccgcgg ctgggacccc gctgaccttg gccccggcct
     1621 ctaccgccca tccggggctc cgaacgccga gcgccatggc caggaccccc aggttaacag
     1681 agacggtggt ctggtatctc acctcgaggt ccaacccggg ggcggatatc atcttgggga
     1741 taccaatggc tccgaacgag gacattccca accaaatacc atcggcccct gagaggccgt
     1801 ccagatatgc ccggaaggga tttccgaagg atgcgaccac gacacctgcc gatccgacca
     1861 ggtcatgtag ggtgtcccgc agcgttccgc atcgccaaac agcctccatt cgcattccga
     1921 ccttagcagt ggacgaggcc tcggtgaccg ccacctcgac ataagcccct attacgacct
     1981 ccttttcaag ggcctcggag gagagcctct ctccgaaacg gaggatcgct gtctcaagtg
     2041 tcagagcgag gccttccatg ctcgggcccg tcgacgttag ctggtagatg gattcggtca
     2101 cggccgccgc gaaggcctgt tggagagggt cgaggtcaaa gcgggacagg tggtcctttc
     2161 cgttcgaagt ccccttcgtc ccgtcccgga actcccatct atccacgcca tccttagccc
     2221 tttgccaggt ccttccatcg ttccagcagt ccgtggagaa gggcgtgctg tccaccctgc
     2281 gcccgtgctc gtccctcagt acgatgccct cctcgtaggg gaatccctcc cccatattgt
     2341 ccaatgcttg gcccgggaag tgatggacag ttcgggaacc gggcaggagc acgccgctca
     2401 gcgggagcgc gccctgcacc ccatgccggg tctctaagga ccagccagcg agatcgacga
     2461 cctgggaggt gggattgtag agctctaccc attccctccc tgcgtcctct ccggccgggt
     2521 tcagctcgta ctcgtttatc accacatcgt ccgttcgacc ttccaggacc atcagggaaa
     2581 tgccggcgtc cacggagccc ttgaggccgg ggatcggaag ggggatgttt gccagcagcg
     2641 cccccacccc ggggatgtcg gagagggcaa aggtcacctc ccggtagcaa aggacctctg
     2701 gcattaccag ctccacacag gcgtcgtcca tgatccccct gacctccacc atgtgctgga
     2761 agacgtccat gaacgggtcg atcaccgcgt ggaccgacca tccatcccca agtagggaag
     2821 cgttcgcaag caggctcatc tcctcgggcc ctttgatcag gcgggtagtg acgtctatcc
     2881 tacagccatt ggccctgaag gccatggaga atgaggcggg gatgcttacc tcccggtaca
     2941 tcatgtcccg tggcctgaca tctatcacca tctccacccc gaggaagcag aggcggaagg
     3001 acctctcccc tattatatcg agcaggacct cggcgagctt ctcctcgatc gtcgagatgg
     3061 ccttccgaag gaagtcctgg agctgttcga cgagggcgcc cagcttggcc gaaaggattt
     3121 cgagcacctc cttgccagtg tcgatcagcc tgtcgatcgt gctctgaatc agccgataga
     3181 ccgccctcag cccggaggaa atgtcctcaa gcccttcccg tatggggcgc agcagccctt
     3241 cgatgacatt ggacaggtgg tcccccaagg tgttgatgtt aacgtaatcg acccccatga
     3301 gggcgtgagc ggtgagcacg gtgacggtct gctccgatcc aagcgcgatc cctcttcgca
     3361 gctccatcgg gaccacctcc ccggcctctc cccctgagag cacggtcaca tccacctccc
     3421 cgtcgaagga aatgccccat gtggattgga aggaggcggt cttcatgctg acgaggtccg
     3481 tatcgtgcgt ttgaggttcc ccctgccccc cctggaaatg gcggggatcg gcgatggaca
     3541 ccaccagcct ttctcccggt ccgttcatgc ttgggtatga gacctgtact tgcagggact
     3601 cgtgatagac ccgcccgtcc gtaccgagca gggcgaaccc gggggtggct ggaatgtcga
     3661 cctccacctg gttccctcgc agcgaaagcc cgtgggatat ctccgacagg aggtcgttca
     3721 catcatcggt caggacaacg ccgagcccgg ccgtggggtc ccctgtcctg gcgatgacct
     3781 cggccaggca tgaactgcga ccatccccgc tccccgcgct cagcccacga acgaactgct
     3841 cctcgaggaa ctccgcacgc cgttcgaacg cgtcctccat cgcagcgtag acgccccagg
     3901 ggccgtcctc gatgatacgg ccttcgatgg cccttaggtt ctcatcccaa agcagggagg
     3961 cgtccggcat ggatgccatc gccgtgctca acaattgctc agccacgctc gagaccaggg
     4021 tgtcgatgac ctcctgcctt cgcaggatat cgtcccggtt ctccctcagc tcagccttgg
     4081 cggcctcggc catcgggtcc atgctagtga ccgatacctc tgccgcggcg atcgccggtc
     4141 taagccatct ctccttattg cacagggcat cggagaggga ggcctttatc tcctctagga
     4201 agggcttgcc gtccctgggg tccaaagaga ggatggtcga agggaggaac aggcccattg
     4261 agatctgttc cgcgaccgca tagatctcgc ttcgtatggt gctgatgatc tccttggtgc
     4321 ccttcttgta ctgctcatgg aaatcgcccc atccctccca ggccatgatg tcgaccttcg
     4381 gtatgtccag gacgacatcg cctccgatca gggctgggac ctcctcccaa tcctcatcta
     4441 ggaggagcat tgtgcctggc cgtacctgga tggtttcctc gggtccatcg gagccgagat
     4501 agcgataaag ctgctcgggg accccggacg cctcgaactc ctcttccaac cattccttga
     4561 accgcctttc cgccatgttc acgtccagga catcgtcgat gagatcgttg acagcgaaat
     4621 agatcttctc cccgacagtc tccacgatct ctagcagatc gatcaggtgt aagtgctcca
     4681 tccacctcag ggcgatcttg tcaacggcag agtacagcgc ctgggagaag accctgcgcc
     4741 aatccagctc atcatagccg tacagcccca agaacaggtc cgcgggatcg atccttccgc
     4801 ctccggacaa ggccgcgctc acataatccc agcacccctg tccgtccagg gcgtccgtgc
     4861 gcatcccgag gcagggggat gcgcttcgga acacctccat ctgtaacaac accagcccga
     4921 ggtctagggc gttgaggagg tccctctccg tcagcgtctc ctccagccct cgggccccgt
     4981 tgacgcttgc tccccatccc tgcagggtgc gataggcggc caaggattcc agcatatagt
     5041 tgaccatgga gctgaggtcc cccaacccgt cgttgacctc tcgttcgaag ctgtccatgc
     5101 ggtccttcag cagcggccaa gggacctggc ccagcccttt caaaggataa tc
//
LOCUS       DAOSYN010000090         4915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_14284_length_4915_cov_3.779218, whole genome shotgun sequence.
ACCESSION   DAOSYN010000090 DAOSYN010000000
VERSION     DAOSYN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14284_length_4915_cov_3.779218"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..1399
                     /locus_tag="PLJ11_04760"
     CDS             476..1399
                     /locus_tag="PLJ11_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04015.1"
                     /translation="METKVASDVHPLFYDHLNAIGRQDRIDLFLYTTGGLTNSAYALV
                     NMIKEYCDHFGIIIPFKALSSGTLMALGADEIVMTRMGQLSPIDPTITTPHGPQVMLP
                     GKPAPDWIPISVEDVNSYINLAKREFGIKDQEGMIKVFDRLSQTINPLALGAVNRVRE
                     QIEFLGLTLLNGHSSDQEKNRKIVETLIRGRYSHDYIINRREARDVIGLNVIDIGPSL
                     EDKIIDLFRQYQEVMALNIPYHPEQALNGKEEVRAVFNRAIIESTVRTNVFRTERTIK
                     RMYAQQPGVPMSTMGYAEESHPDRWVVDENL"
     gene            1436..1681
                     /locus_tag="PLJ11_04765"
     CDS             1436..1681
                     /locus_tag="PLJ11_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04016.1"
                     /translation="MDRPLSVWKESSSGLANGSQLVDSSSSVTPAVLDVTPVQSVPVV
                     FHSATPAIIRSRREIFEDAIGVTLSRNKVALDELGKL"
     gene            1690..2106
                     /locus_tag="PLJ11_04770"
     CDS             1690..2106
                     /locus_tag="PLJ11_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HOO04017.1"
                     /translation="MAPAQILFFLDSEDILAIHERLMKDQEENTRGLLSPQNLDMCVK
                     SPNHRMFGIEPYDDLIKKGAKLAFEIVCLHPFVDGNKRTALSALSIFLEMNGYSLIVP
                     SRTAVHVMVEVAKGSADFESFLIFVKEHTTAKEPLQ"
     gene            complement(2123..>4915)
                     /locus_tag="PLJ11_04775"
     CDS             complement(2123..>4915)
                     /locus_tag="PLJ11_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04018.1"
                     /translation="VHGSLVTIAEAVEERTRAAVGERLAEAEAFIARCAPGPGTDRAR
                     AALAEARGRLARKEYEGAHALAGELMERLMAERREGLDRSLGEGRELLEMAKRLGADS
                     VILRDKLQRAEELRALGRTDEAAALAEEVRGYGRGIVSSEIDQRLTGIVRRMALARRE
                     GVEVGGPERMAEQASGALQHGDLGQAFELALAAKNALDEVVGTHRRLRARLEEGKRVL
                     AEGRRAGVDMAEARGMMERAEALLGAGDLKGATEELDRASVLVRTKAPDLLLEYRLRE
                     MVELARLRERLGRKGGEAELAGLGPLDAAHLQGSLEALARLREEWRREVAEALRAALD
                     DRQREVERATARGYCVGPAQQMVLQGRAALDEGRLEEAARVLDVVRTELDEGLQADRR
                     LSEALAALEDAVGQLTELKADARDATALLEQAKALRRGGDVARAEEHARRGLERADGI
                     AALLVGQLAAFAGGLTPEREEWEDLRPARKLHEDMEAALRARRYRHAHLLARSFREEL
                     ERTLQDKAQAEDAVREFEARLEEEAKAGLRPPALERTLERAKALLAQGRFVQAMGTVA
                     AAGDELRALSEMYEARLKEYNELREALNSLEVLDARKDHIEGLLEQGLSSLRELRFDS
                     ASLFLRRARTALNEFLATRTNELLWEFTPLHDLMKRFKAQRRFAAEVAEIERTTVDGI
                     DPRALNRLARNVELVRAGLGDIFQEQREKARRAIERAARAGKEAGRAWEVWAEAEAQA
                     GKGDLWGAFTSLEAAVNAIGRKAGEAPEQLSKQVAEVLERAGRERVRLPETERAYAEA
                     LQAGRDGGADAELLRRALETSRNEVRATCPRIAVDAELAGDAKEGRPMDVLLRVTNTG
                     EYAARAVRAFLFGDAEVRGMLEAEALAPGESAEGRITVIPNRVGILSLGISVKCRPLL
                     TDEDILYDSKLDLDVK"
BASE COUNT          846 a         1749 c         1393 g          927 t
ORIGIN      
        1 cggtgagagc ctttaatcgc tatggttccc acccccgaca ccatgatcct gtgatccaat
       61 ggtgctgagc ttcaaaaaca agtaagtggc caatcccgaa tgtatcaata aaaggccaat
      121 ggttcctatc acgctgtcca acggtgatgt ctccttcgag acgctcgccc tccttgactc
      181 aggagccgac gcctcggcga tatcgaggga aatggccgag gtgcctggtt tgggcatcag
      241 tgtatatatt gagcattggg ttcaagccgg gcttcctttg aacatcgacc ccctgcattt
      301 ccgatgttaa tatccgatcg ccgaactatt tgtaatggaa tcctttaatc tgcaataatc
      361 tatgaacatc taaggtggtc tcatgggaag ggctcaaaga ctggcattgt tacgttcatt
      421 ggagaagaaa cggaagagta aagtgatagc atatctagcc ggcgaccgtc ccggaatgga
      481 gaccaaggtg gcttctgatg ttcatccttt gttctacgac caccttaatg cgattgggcg
      541 tcaggaccgg atcgacctgt tcctatacac caccggaggg ctgaccaatt ccgcctatgc
      601 attggtcaac atgatcaaag aatattgcga tcattttgga ataatcatac ctttcaaagc
      661 tttgtcgagc ggtactttga tggctcttgg ggcggacgaa atagtcatga ccaggatggg
      721 tcagctatcg ccgatcgatc caacgataac cactcctcac ggaccacagg taatgttgcc
      781 tggaaaaccc gctcctgatt ggatcccgat cagtgttgag gacgtcaaca gttacatcaa
      841 cttggcaaag cgcgagttcg ggatcaaaga tcaggaaggg atgatcaagg tcttcgatag
      901 attgagccaa acgatcaatc cgctcgctct tggcgctgtt aacagggtaa gggaacagat
      961 agagttcctc gggctcaccc tgctcaatgg acattcctcc gaccaagaaa agaacagaaa
     1021 gatcgtggag accctgataa gggggcggta ttcccacgat tatatcatca acaggaggga
     1081 ggccagggac gtcattggtc tgaacgtcat tgatatcggt ccttcgttgg aagataagat
     1141 catcgatctg ttcaggcaat accaggaagt gatggcattg aacatcccct atcatccgga
     1201 acaggctctc aatggaaagg aggaggtcag ggcggtgttc aacagggcga tcatcgaaag
     1261 cacggtgcgc accaacgtct tccggaccga gaggacgatc aaaagaatgt acgcccagca
     1321 gccaggtgtt cccatgtcaa ccatgggcta tgccgaggaa tctcatcctg ataggtgggt
     1381 ggtcgatgaa aacttataag tcatccatgc attatggaaa ttcggagggg ctttcatgga
     1441 caggccattg agcgtttgga aggagagcag ttccggtttg gctaacggga gccaactggt
     1501 cgacagctca agctccgtta cacctgcggt cctcgatgta acccccgtcc aatcggtgcc
     1561 tgtggtgttc cactccgcta ccccggccat catcagatct cgaagagaga tcttcgagga
     1621 tgccatcggg gtaacactgt ccaggaacaa ggtcgccttg gacgagctcg gaaagctcta
     1681 ggtgttctct tggcacccgc ccaaatcctt ttctttcttg attccgagga catactggcg
     1741 atccatgaaa ggttgatgaa ggatcaagag gagaacactc gcggtctgtt gtcccctcag
     1801 aatctggaca tgtgcgtcaa atctccgaac cacaggatgt tcggcatcga gccgtatgat
     1861 gatctgatta aaaaaggcgc caagctggcg ttcgagatcg tctgtctgca cccgtttgtc
     1921 gacggcaata agaggaccgc cctatcggcg ctctccatct ttctggaaat gaacggatat
     1981 tcattgatcg taccatcgcg gacggcggtt catgtcatgg tggaggtagc gaagggttcg
     2041 gcggactttg aaagtttcct aattttcgtg aaggaacata ccacggccaa agaaccgctt
     2101 caatgagaga tgatctttag gatcacttca cgtccaggtc gagcttgctg tcgtagagta
     2161 tgtcctcgtc cgtcagcagg ggcctgcatt taactgatat ccccaaggat agaatgccga
     2221 cgcggttcgg gatgacggtg atgcgcccct ccgcgctctc cccgggcgcg agcgcctccg
     2281 cctcgagcat cccgcgcacc tccgcgtccc cgaagaggaa cgcgcggacc gcccgcgccg
     2341 cgtactcccc cgtattcgtc acgcggagga ggacgtccat cggcctcccc tccttcgcgt
     2401 cccccgcaag ctccgcgtcg acggcgatgc ggggacaggt cgccctcacc tcgttcctcg
     2461 aggtctcgag cgccctcctc aggagctccg cgtcggcccc gccgtcccgc cccgcctgca
     2521 gcgcctccgc gtacgcccgc tccgtctccg ggagccggac ccgctcccgg cccgcccgct
     2581 cgagcacctc ggccacctgc ttggagagct gctccggggc ctcccccgcc ttcctcccga
     2641 tggcgttcac cgccgcctcc agggaggtga acgcgcccca gaggtccccc ttcccggcct
     2701 gcgcctccgc ctccgcccag acctcccatg cccgtccggc ctccttcccc gcgcgggcgg
     2761 cccgctcgat cgcccgcctg gccttctccc tctgctcctg gaagatgtcc cccagcccgg
     2821 ccctcaccag ctccacgttc ctagcgagcc ggttgagagc gcgcgggtcg atgccgtcca
     2881 ccgtcgtccg ctcgatctcc gcgacctccg ccgcgaaccg cctctgcgcc ttgaaccgct
     2941 tcatcaggtc gtgcagcggg gtgaactccc agagcagctc gttcgttctg gtggccagga
     3001 actcgttcag cgcggtccgc gccctccgga ggaacaggga ggccgagtcg aaccgcagct
     3061 ccctcaggga cgaaaggccc tgctcgagca gcccctcgat gtggtccttc cgcgcgtcga
     3121 gcacctccag ggagttgagc gcctccctca gttcgttgta ctccttcagc cgggcctcgt
     3181 acatctcgga tagcgcccgg agctcgtccc cggcggccgc caccgttccc atcgcctgga
     3241 cgaagcgccc ctgcgccagg agcgccttgg cgcgctccag cgtcctctcc agcgccggag
     3301 gcctcagccc cgccttcgcc tcctcctcca ggcgcgcctc gaactcgcgg accgcgtcct
     3361 cggcctgcgc cttgtcctgc agcgtccgct ccagctcctc gcggaacgac cgcgccagga
     3421 ggtgcgcgtg ccggtagcgc cgggcgcgga gcgccgcctc catgtcctca tgcagcttgc
     3481 gggcggggcg caggtcctcc cattcctccc tctccggcgt caggccgccg gcgaaggccg
     3541 cgagctgccc caccagcagg gcggcgatgc cgtccgcgcg ctccagcccc ctccgggcat
     3601 gctcctccgc gcgcgccacg tccccgcccc tccgcagggc cttggcctgc tccagcaggg
     3661 ccgtcgcgtc cctcgcgtcc gccttgagct cggtcagctg cccgaccgcg tcctccaggg
     3721 ccgcgagcgc ctcgctcagc cgccggtcgg cctgcagccc ctcgtcaagc tccgtccgga
     3781 ccacgtccag gacgcgcgcc gcctcctcga ggcgcccctc gtcgagcgcc gctctgccct
     3841 gcagcaccat ctgctgcgcg ggcccgacgc agtaccccct agccgtcgcc cgctccacct
     3901 cccgctggcg atcgtcgagc gcggcgcgca gggcctcggc gacctcccgc ctccattcct
     3961 ccctgagccg ggcaagcgcc tccagggacc cctgcaggtg cgcggcgtcg agggggccga
     4021 gccccgccag ctcggcctcg ccgcccttcc gccccagccg ctcccgcagg cgggcgagtt
     4081 cgaccatctc cctgagcctg tactcgagga ggaggtccgg ggccttcgtt cggactaaaa
     4141 cgctggcccg gtccagctcc tccgtcgccc ccttgaggtc cccggcgccg aggagcgcct
     4201 ccgccctctc catcatcccc ctggcctcgg ccatgtccac gcccgcccgc ctgccctcgg
     4261 ccaggacccg cttcccctcc tccaggcgcg ccctcagccg cctgtgcgtc ccgacgacct
     4321 cgtccagcgc gttcttcgcg gcgagggcga gctcgaacgc ctgccccagg tccccgtgct
     4381 gcagggcgcc cgaggcctgc tcggccatgc gctccgggcc cccgacctcc acgccctccc
     4441 tgcgcgcaag cgccatccgc cggacgatcc cggtcagcct ctgatcgatc tccgaggaga
     4501 cgatcccgcg gccgtagccg cgcacctcct ccgcgagcgc ggcggcctcg tccgtccggc
     4561 ccagggccct gagctcctcg gcgcgctgca gcttgtcccg gaggatgacg ctgtccgccc
     4621 ccagccgctt ggccatctcc aggagctccc tcccttcgcc gagcgagcgg tcaaggccct
     4681 cccggcgctc ggccatcagc ctttccatca gctccccggc gagggcgtgc gccccttcgt
     4741 actccttccg cgccaggcgc cccctcgcct ccgccagggc cgccctcgcc ctgtcggtcc
     4801 cgggcccggg ggcgcaccgg gctatgaacg cctccgcctc ggcgaggcgc tcgcccaccg
     4861 ccgcccgcgt ccgctcctcc accgcctcgg cgatggtcac cagggagccg tggac
//
LOCUS       DAOSYN010000091         4913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_14297_length_4913_cov_4.161383, whole genome shotgun sequence.
ACCESSION   DAOSYN010000091 DAOSYN010000000
VERSION     DAOSYN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14297_length_4913_cov_4.161383"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLJ11_04780"
     CDS             <1..576
                     /locus_tag="PLJ11_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOO04019.1"
                     /translation="PLDAPDAPEYVEIDFRKGLPAGLDGTEMPMTDIIKRLNQLGGKH
                     GYGRVDHIEDRLVGIKSREVYEAPAALMLINAHKDLEKMVLTKDVLNFKRGVEQRYSE
                     LCYDGLWFSPLKNALDAFVAETQEHVEGTVRVKLYKGSATIVGRSSPYSLYDTGLATY
                     AQGDRFDHRSAEGFIYVWGLPLRTVAKAQKK"
     gene            582..2027
                     /gene="argH"
                     /locus_tag="PLJ11_04785"
     CDS             582..2027
                     /gene="argH"
                     /locus_tag="PLJ11_04785"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOO04020.1"
                     /translation="MAERQLWSGRFAEGPSEMTLSFTSSLAVDARLAWYDVAGSLAHA
                     RMLAATGIVPKEAAEALIDGLKQLLDDLEAGKIELSDELEDVHTNIEHLLTERVGEAG
                     KRVHTARSRNDQVATDLRMFVRDAVLEIITLLIDVQEVLIERAEEELGTIMPGFTHMQ
                     HAQPVSLGFHLMAHVFRLQRDAERFLDAYKRLNQCPLGAGALAGTGHPVDRALTAALL
                     GFDRPTDNAMDTVSDRDFALEFVYACAQTMVHLSSMGEELVLWTSPEFRFAEMPDAFA
                     TGSSIMPQKKNPDVAELVRGRSSIAIGNLVQMLTMMKGLPLSYNRDMQEDKPGVFSSY
                     ETLTSCLYVLAPMYRSLAFDRERMEEACAEGFLNATDLADHLVQKGMPFRQAHEVVGA
                     AVQYCLRTGKRLESLSAEELKGLSDLLDEGSLAHISMEACLNRRTSKGGTAPSAVREQ
                     VACARAIIDSHRSVCQGEIGRLESVWKELRK"
     gene            complement(2032..2400)
                     /locus_tag="PLJ11_04790"
     CDS             complement(2032..2400)
                     /locus_tag="PLJ11_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04021.1"
                     /translation="MKASSGHLVLSDSVEYQDMAGVFPEVLKLFLEETDQVPEDPEIM
                     QMFIHLNMMDLEANRKPEGYNRKGRMRLIFPTNRKEFYVKGSSKSGEVVRVTEKISKL
                     LTRNGVEHELEWNTLSFSDS"
     gene            2504..3814
                     /gene="thiD"
                     /locus_tag="PLJ11_04795"
     CDS             2504..3814
                     /gene="thiD"
                     /locus_tag="PLJ11_04795"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOO04022.1"
                     /translation="MTVILTVAGSDSVGGAGVEADVRAIASMGGHAAVALTAVTAQNT
                     ARLRDVFPLPPEQVTAQITAVAEDARIAAVKTGMMFSARTVRAVAPLLMELKAPLVVD
                     PVLFAGVGASLHREGLREAMVKDLFPLATVVTPNRSEAEAFSGMRIEGPGSLEDVGNR
                     LLEMGPEAVLIKGGHLEGDLAVDSLFTAEGVLEVASPRLDRRVHGAGCTFSAFIATGL
                     GIGMGLREAVREAKRRIYDSVAMSVPVGKGLLAIDPMATLHKEAMRAGVIEEVRKAVA
                     VIEERLPPELVPEVGMNLAFALPYPQGYGEICGVEGRLVRVGDKVRRVGEVRFEGSRH
                     MARVVMAASFVDPEVRCAMNIRFTEAVVDRLRATGAMVGTFDRGEEPAMVSSMEWGTA
                     EAIRGCGHVPDVIYDRGGAGKEAMVRVLGRDPDDVLRKVSALMR"
     gene            3825..>4913
                     /locus_tag="PLJ11_04800"
     CDS             3825..>4913
                     /locus_tag="PLJ11_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO04023.1"
                     /translation="MKIAMFTDSYLPTRDGVVTSLLLTKRELERMGHTVYVFAPDPAD
                     PSDREEGVHYFRSIGFHRYSGYRIPLFPTDKCHILEGLDVDLIHVHGLMFQAVRGMLA
                     GRALRKPVVLTFHTMVTEAARFYNFTPFPEWVIQLGMWTYLRTILHRAEVVIAPTVAI
                     KNELKRYAPHIKRLEVIPTGVDLDRFNPRIDGSEVRRRYGLEGKKVILHLGRIAWEKN
                     IDLVVRGFALLSAHEPEARLLLVGEGPAKEHVRGLVKELGIEERVIFTGFVPDPELPQ
                     FYAACDVLTLASKFETQGLVILEAMAVGKPVSGIRYRAVAELVREGENGELFEETPYS
                     WSQATLRLLRDPERYREGALAKAREFSAG"
BASE COUNT          870 a         1475 c         1689 g          879 t
ORIGIN      
        1 ccgctcgacg cccccgatgc cccggagtac gtcgagatcg acttcaggaa ggggctgccc
       61 gccggcctcg acggcaccga gatgccgatg accgatatca tcaagcggct caatcagctc
      121 ggtgggaagc atggctatgg cagggtggac catatagagg accggctggt cggcatcaag
      181 tcccgggagg tctatgaggc cccggccgcg ctcatgctca taaacgccca caaggacctg
      241 gagaagatgg tcctcaccaa ggacgtgctc aacttcaagc ggggggtcga gcaacgctat
      301 tccgagctct gctatgatgg gctatggttc tccccgctca agaatgccct ggacgccttc
      361 gtggctgaga cccaggagca tgtcgagggc acggtgaggg tgaagctcta caagggcagc
      421 gccaccatcg tggggcgctc ctcgccgtac tcgctgtacg ataccgggtt ggcgacctat
      481 gcgcaggggg atcggttcga ccatcggtcc gccgaggggt tcatctatgt ttggggactg
      541 ccattgcgca cggtggccaa ggcccagaag aagtgatccg aatggcagag aggcagctct
      601 ggtccggtcg gttcgccgag ggcccttccg agatgaccct ttccttcacc tcctccctgg
      661 ccgtggacgc gcggcttgcg tggtacgacg tcgccggctc cctggcgcac gcccggatgc
      721 ttgcggcgac ggggatcgtg ccgaaagagg cggcggaggc attgatagat ggcctcaagc
      781 agctgctcga cgaccttgag gctggaaaga tcgaactctc cgacgagctg gaggacgtgc
      841 acaccaacat cgagcatctg ctcactgagc gggtcggaga ggcgggcaaa cgggtgcata
      901 ccgcccgcag tcggaacgac caggtggcca ccgacctccg catgttcgtt cgcgatgccg
      961 tccttgagat catcaccctg ctgatcgacg tccaagaggt cctgatcgaa agggccgagg
     1021 aggagctcgg gaccatcatg ccgggcttta cccacatgca gcacgcccag cccgtgtccc
     1081 tggggttcca tctcatggcc cacgtcttcc gcctgcagag ggatgcggag aggttcctgg
     1141 acgcctacaa gcgcctcaac caatgcccat tgggcgcagg ggccctcgcc ggcactggac
     1201 atcctgtgga ccgcgccctg acagcagccc tactgggctt cgatcgcccg accgacaacg
     1261 ccatggacac ggtgagcgac cgggacttcg cacttgagtt cgtctatgcc tgcgcccaga
     1321 ccatggtgca cctgagcagc atgggggagg agcttgtgct ttggacctcc cctgagttcc
     1381 gcttcgcgga gatgcccgac gccttcgcca ccggcagctc catcatgccc cagaagaaga
     1441 acccggacgt ggcggagctg gtccgtggac ggagctccat cgccatcggg aacctggtgc
     1501 agatgctgac catgatgaag gggctgccgt tgagctacaa cagggacatg caggaggaca
     1561 agcccggggt gttctccagc tacgagacgc tcacctcctg cctgtacgtc ctcgccccca
     1621 tgtaccgctc gctggccttc gaccgggagc gcatggagga agcatgcgct gaagggttcc
     1681 tgaacgccac cgacctggcc gaccatctcg tccagaaggg gatgcccttc cgacaggccc
     1741 atgaggtcgt gggcgcggcc gtgcagtact gcctgcggac cggcaagcgc ctggaatcac
     1801 tgagcgcgga ggagctgaag gggctttccg acctcctcga cgagggctcc ctcgctcata
     1861 tttccatgga ggcatgtctc aaccggcgca cctcgaaggg gggcacggcc ccctcggcgg
     1921 tccgagagca ggtagcctgc gccagggcca tcatcgattc gcatcggtcg gtgtgccaag
     1981 gggagatagg tcggctcgag agcgtttgga aggaactccg gaaataggcc ctcagctgtc
     2041 cgagaaggaa agggtgttcc actccagctc atgctcgacc ccgttccggg tgagcagctt
     2101 gctgatcttc tccgtgaccc ggaccacctc tccgctcttg gaggagccct tgacatagaa
     2161 ctccttcctg ttcgtgggga agatgagccg catccgcccc ttgcggttat agccctcggg
     2221 tttgcggttg gcctccaggt ccatcatgtt caggtggatg aacatctgca tgatctccgg
     2281 gtcctcgggg acctggtccg tctcctccag gaacagcttg agcacctctg gaaagacccc
     2341 ggccatgtcc tggtattcga cgctgtcgct cagcaccagg tggccactgc tcgctttcat
     2401 cggattttcg aacagaaacg aattataaat aagttcgccc catggccctg gacggggcca
     2461 ttggtcgcct aaggttatta agagggctga ccttacacca tctatgaccg tcattctgac
     2521 cgtcgcgggc tcggactcgg tgggcggggc gggcgtggag gccgatgtac gggccatcgc
     2581 ctctatgggg gggcatgcgg cggtcgccct gaccgcggtg accgcccaga acaccgcccg
     2641 gctgcgggat gtattcccat tgccacctga acaggtcacc gcccagatca ccgcggtggc
     2701 cgaggacgcc cgcatagcag cggtgaagac cggcatgatg ttctccgccc gcacggtacg
     2761 ggcggtcgcc cccctgttga tggagctcaa ggcgcccctt gtcgtggacc cggtgctctt
     2821 cgccggggtg ggggcgtccc tgcaccggga ggggttgagg gaggcgatgg tgaaggacct
     2881 ttttcccttg gcgacggtgg tcacccccaa ccgcagcgag gcggaggcct tctcggggat
     2941 gaggatcgaa ggccctggct cgctcgagga tgtggggaac cgtctgctgg agatggggcc
     3001 cgaggccgtg ctcatcaagg gcgggcatct ggagggggac ctggccgtcg actcgctgtt
     3061 cacagcggag ggcgtcctcg aggtggcctc tccccggctt gaccgcaggg tgcatggcgc
     3121 gggctgcacg ttctccgcgt tcatcgccac agggcttggg attgggatgg ggctcaggga
     3181 ggccgtaagg gaggccaagc gccgcatcta cgactcggtg gccatgtcgg taccggtggg
     3241 aaaggggctg ttggcgatcg acccaatggc cacgcttcac aaggaggcca tgagggctgg
     3301 ggtgatagag gaggtgagga aggccgttgc cgtgatcgag gaacgcctcc cgcccgagct
     3361 ggtgcccgag gtgggaatga acctcgcgtt cgccctgccc tatcctcagg ggtatgggga
     3421 gatatgcggg gtggagggcc gattggtccg ggtcggggac aaggtccgca gggtggggga
     3481 agtgaggttc gaagggagcc ggcacatggc gagggtggtg atggccgcct ccttcgttga
     3541 cccggaggtg cggtgcgcca tgaacatcag gttcacggag gccgtcgtgg acaggctgag
     3601 ggcgacgggg gcgatggtcg ggaccttcga ccgaggggaa gagccggcga tggtgtcctc
     3661 gatggaatgg ggcaccgcgg aggccataag gggatgcggt catgtgccgg acgtcatcta
     3721 cgatcgcgga ggcgcgggga aggaggccat ggtccgggtc cttggccggg accctgatga
     3781 tgtgctccgc aaggtctcgg cgctgatgcg atgagggtgc ggccgtgaag atcgccatgt
     3841 tcaccgattc ctatcttccc acccgggacg gggtggtcac atccctgctg ctcaccaaga
     3901 gggagctgga gcggatgggg catacggtct atgtcttcgc ccctgatccg gcggacccct
     3961 cggacaggga ggagggcgtc cactacttcc ggtccatcgg gttccacaga tactccgggt
     4021 accgcattcc cttgttcccg acggacaagt gccacatatt ggaagggctg gatgtcgact
     4081 tgatccacgt tcatggcctt atgttccagg cggtgcgggg tatgctggcc ggcagggcgc
     4141 tccggaagcc ggtggtgctc acgttccata ctatggtcac agaggcggcc cgcttctaca
     4201 acttcacccc cttcccggaa tgggtcatcc agctggggat gtggacctat ctgcgcacga
     4261 tcctgcatag ggcggaggtc gtgatcgccc ccaccgtcgc cataaagaac gaattgaagc
     4321 gctacgcccc acacatcaag cgcctggagg tcatcccgac cggggtggac ctggatcggt
     4381 tcaaccccag gatcgatggg agcgaggtcc ggaggcgcta cggcctcgag gggaagaagg
     4441 tcatcctgca cctggggcga atagcctggg agaagaacat cgatctggtc gtcaggggct
     4501 tcgccctgct cagcgcccat gagccggagg cccgcctcct tctcgtcggg gagggcccgg
     4561 ccaaggagca tgtgcggggg ctcgtgaagg agcttggcat cgaggaaagg gtgatcttta
     4621 ccggcttcgt tcccgacccg gagctgcccc agttctatgc ggcatgcgac gtgttgacgc
     4681 tggcgagcaa gttcgagacc caggggttgg tcatactgga ggccatggcc gtcggcaagc
     4741 cggtcagcgg cataaggtac cgggcggtgg ccgagctggt ccgcgagggg gagaacgggg
     4801 agctgttcga ggagacccca tacagctggt cccaggccac gcttcggttg ctgagggacc
     4861 ccgagcgcta tcgggagggc gccctggcca aggcgaggga gttctccgcg ggg
//
LOCUS       DAOSYN010000092         4911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_14305_length_4911_cov_3.785008, whole genome shotgun sequence.
ACCESSION   DAOSYN010000092 DAOSYN010000000
VERSION     DAOSYN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14305_length_4911_cov_3.785008"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLJ11_04805"
     CDS             complement(<1..779)
                     /locus_tag="PLJ11_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO04024.1"
                     /translation="MSRQAAMDLAKQLRRRPGDRGGQRILVAGKGGVGKTTLAAALCL
                     LGAEKGRRVLAVDQDAQQNLAFSLGYPKERAAALRSIIQDLDFIEERTGARPGQGWGG
                     LMNLDPDVSDVAERFGVRISDGLYLLVMGGVAQASTGCLCPENALLGALVRFLNSRED
                     DLIVMDAQAGLEPFGRTVAEGFGTCLVVSEPTFNSLQVAGRVAQLSRQMGMHRVDLVL
                     NKCEEGDICKARESGWEGYDDVHVLPWDPSVRENEPDVSRLL"
     gene            complement(776..2716)
                     /gene="cooS"
                     /locus_tag="PLJ11_04810"
     CDS             complement(776..2716)
                     /gene="cooS"
                     /locus_tag="PLJ11_04810"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HOO04025.1"
                     /translation="MSDEQGLEVISVECVRMPPERISPDKVPEKVEERTTDPSAKEVL
                     AKTMLEGVETVWDRFEKQQPSCKFCEAGVSCQRCAMGPCRIMGEGKNRGVCGATADLI
                     VSRNLLDQIATGAAAHSDHGREVAETLLKAAGGEAPQYKISDRAKLMTLAKEYGLDTD
                     GPPEKVAEKLALAMLEEFGTLKGELRMARRLPLETHKMLREAGILPRSIDREIVEAMH
                     RVHMGVGADYRSILAHGVRASMGDGWGGSMIGTEVSDVLFGTPGIRTSMVNLGVLKQD
                     HVNISLHGHNPILSEMVVKAASLPEMKRRAEEAGAKGINLVGLCCTGNELLMRKGIPQ
                     AGNHLSQELVITTGALEAMIVDYQCIFPSLPNTAACFHTKVISTSPKAVIPGSVYVEV
                     TPENAFEQAKRMVAMAVDNFRNRVPERVLIPKAPVDAVVGFSVEALKNAMGGSLKPLL
                     DLILEGKVRGAVGIVGCNNPRIKQDFGHITLAKELIRRNILVVETGCAAVACAEAGLM
                     QLGAADLAGRSLGNACRSLGIPPVLHMGSCVDNTRILTLVAELANLAGVRTDQLPVAG
                     AAPEWYSPKAVAIGCYFVGSGISVALGVMPKIGGSPNVIKLLTDDLERTVKAKFWVEP
                     DPKKAAAILFDHIETKRADLGL"
     gene            2838..>4911
                     /gene="metG"
                     /locus_tag="PLJ11_04815"
     CDS             2838..>4911
                     /gene="metG"
                     /locus_tag="PLJ11_04815"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOO04026.1"
                     /translation="MERSKVFIGVAWPYANSAIHLGHVAGSLLPPDIFSRYHRLKGHR
                     VLMVSGSDQHGTPVTVRADKEGVSPQQVADRYHEINKRAIEDLGIEFSLFTKTHTDNH
                     IQVVQDIFLRLLEKGHMELRETMQYYCPTCAKFLPDRYVEGRCTKCGNESSRSDQCDR
                     CGATFEVGEIADPVCITCNNRPEMRETKHHFFKLTDFEDQLRSYVQVSDHWRSNVQLF
                     TQNWLESGLKDRPITRDMAWGVPVPLPGMEGKVIYVWFEAVIGYLSASKEWAMSTGDP
                     DAWREFWTDPEVKGYYFLGKDNIPFHTIIWPAILMGYGGLNLPYDVPANEFLTFKGEK
                     LSKSKGPSIDIPSILKVFDADQIRYYLASNMPEGKDADFSWEDFETKVNNELVATLGN
                     YFHRVLSFTQKNFHQVPPLGEGLEVETEQVEGAIAQAIAEVDANISSCQFKRGLKAIM
                     DLAQFGNRFFDSCAPWSMLRSDRDRCGGMLHLNLRVVQALAVLAHPYLPFTTQRLWAF
                     LANDGAIGEGSWSEITKPLNVGRELPEPRPLYRKVDIQQEERPFSGFEALDLRVGVVK
                     EVSDHPNADRLMVLKVDIGRPIQLVAGLKAFYTKEELTGKKVVVIANLQPAKLRGVES
                     QGMVLAAEADGEVKVLTPSGPAEPGDRVTSGLSPGGRPLSLQEFQAFVLRTGTVLNDQ
                     EADVGRRGK"
BASE COUNT          938 a         1610 c         1464 g          899 t
ORIGIN      
        1 aggagccggg agacatcggg ttcgttctcc cggaccgaag ggtcccacgg aaggacatgc
       61 acatcatcgt acccctccca cccgctctcc cgggccttgc agatatcccc ttcctcgcat
      121 ttgttgagca caaggtccac cctatgcatc cccatctgac gggatagctg ggccacccgt
      181 ccggccacct gaagggagtt gaacgtcggc tccgagacca cgagacatgt cccgaaccct
      241 tcggccaccg tccggccgaa cggttccagc ccagcctgcg catccatgac gatgaggtcg
      301 tcctcccggg aattgaggaa acggaccaaa gcgcccaaga gggcgttctc cggacacagg
      361 cagccggtgg aggcctgcgc caccccgccc atcaccagca ggtacagccc atccgagatc
      421 cgtaccccga accgctcggc cacgtccgaa acgtcgggat cgaggttcat cagccctccc
      481 catccctggc cagggcgtgc ccccgtcctc tcctcgatga agtccaggtc ctggatgatg
      541 gagcggagag cggccgccct ctcctttgga tagccgaggg agaaggcgag gttctgctga
      601 gcgtcctgat cgacggcaag cacccgacgc cccttctccg cccccagcag acagagggcg
      661 gcagcgagcg tggtcttgcc caccccgccc tttccggcga ccaggatacg ctgtcctccc
      721 cggtccccag gcctccggcg cagctgcttc gccaggtcca tggccgcttg cctgctcata
      781 ggcccaggtc cgcccttttc gtttcgatgt ggtcgaagag gatcgccgcc gccttcttcg
      841 ggtcgggctc cacccagaac ttggctttga ccgtcctctc tagatcgtct gtgagcagct
      901 tgatgacgtt cggcgagccc cctatcttcg gcatcacgcc caacgccaca ctgatcccgc
      961 tccctacgaa gtagcatccg atggccaccg ccttcgggga ataccactca ggggccgctc
     1021 cggccaccgg aagctggtcc gttcgaaccc ccgccagatt ggcaagctcc gcaactaggg
     1081 tgaggatgcg ggtgttatcc acgcatgagc ccatgtgtag caccgggggg atgcccagcg
     1141 aacggcacgc attgcccagg gacctaccgg ccagatcggc tgcgccaagt tgcatcaggc
     1201 ccgcctcggc gcaggctacg gccgcgcaac cagtctccac caccaagatg ttacgacgga
     1261 tgagctcctt ggccaaggtg atgtggccga agtcctgttt gatcctgggg ttgttacagc
     1321 ccacgatccc cacggctcct cggaccttgc cctccaggat caggtccaaa aggggcttaa
     1381 ggctgccgcc catagcgttc ttcaaggctt ccacggagaa gcccaccacg gcatcgaccg
     1441 gcgctttggg gatgagcacc cgttccggca cgcggttccg gaagttgtca acggccatgg
     1501 ccaccatgcg cttcgcctgc tcgaaggcgt tctccggggt gacctccacg tatacgctcc
     1561 ccgggatcac ggccttgggg gaggtggata tcaccttggt atggaagcag gcagcggtgt
     1621 ttggaaggga tggaaagatg cactggtagt ccacgatcat ggcctccagg gccccggtgg
     1681 tgatgacaag ttcctggctg aggtggttcc ctgcctgggg gatgcccttc ctcatcaaca
     1741 gctcgttccc cgtgcagcag aggccgacaa ggttgatgcc cttcgctcca gcctcctcgg
     1801 ccctcctctt catctccggg agcgaggcgg cctttaccac catctcggag aggatggggt
     1861 tatggccgtg caaggagatg ttcacatggt cctgcttgag cacccccaga ttgaccatgc
     1921 tggtgcggat gccgggggtt ccgaacagaa cgtcgctcac ctcagtgcct atcatggagc
     1981 caccccaccc atcccccatc gatgcccgga ccccatgggc caatatgctc cggtagtcag
     2041 cgcccacgcc catatgcacc cggtgcatgg cctccactat ctccctgtcg atcgacctgg
     2101 gaaggatgcc agcctccctc agcatcttgt gcgtctccaa gggcagccgt ctggccatcc
     2161 tcagctctcc cttcagggtg ccgaactcct cgagcattgc aagggccaac ttctccgcga
     2221 ccttctccgg cggcccatcg gtgtcaagcc catattcctt ggccaatgtc atcagcttgg
     2281 ctcggtccga tatcttgtat tgcggtgcct cgccccctgc cgccttgagg agggtttcgg
     2341 ccacctcccg gccatggtcg gaatgggcag cggcgccagt ggctatctgg tccaaaaggt
     2401 tcctcgagac gatgaggtcg gccgtggccc cgcatacccc ccggttcttc ccctccccca
     2461 tgatgcggca cggacccatg gcgcaccttt gacaggatac gcccgcctcg cagaacttgc
     2521 aggacggctg ctgcttctcg aagcggtccc ataccgtctc caccccctcc aacatggtct
     2581 tggccaggac ctccttcgcc gaagggtccg tcgtcctctc ttccaccttt tccggcacct
     2641 tgtccggaga tatgcgctca ggcggcatcc tcacgcattc tacggatatg acctcaagcc
     2701 cttgctcgtc cgacatcggt tcaccttccg ccatccctgc gatggctcag gtatcaatgg
     2761 tcattccaga atatttgaac ctgtggattt tcccctctga cggcaaggtt atattcaaaa
     2821 agtccattcg tctgccgatg gagcgctcaa aggttttcat cggggtggcc tggccatacg
     2881 cgaacagcgc catccacctg gggcatgtgg ccggttcgct gctacccccc gacatattct
     2941 cccgctatca cagattgaaa gggcaccgcg tgctgatggt ctctggctca gaccagcacg
     3001 gaactccggt caccgtccga gcggacaagg aaggggtgag cccccagcag gtggcggaca
     3061 ggtaccacga gatcaacaag agggcgatcg aggacctggg catcgagttc tcgctgttca
     3121 cgaagaccca cacggacaat cacatccagg tcgtgcagga catcttcctg cggttgctgg
     3181 agaagggcca catggagctt agggagacga tgcagtacta ttgccccacc tgcgccaagt
     3241 tcctccccga caggtacgtc gaggggaggt gcaccaagtg cggcaacgag agcagcagga
     3301 gcgaccaatg cgatcggtgc ggggcgacgt tcgaggtggg ggagatcgcg gacccggtgt
     3361 gcatcacctg caacaaccgc cccgagatgc gggagacaaa gcatcatttc ttcaagctga
     3421 cggacttcga ggaccagctg cgctcctatg tccaagtgag cgaccattgg cgctccaacg
     3481 tccagctctt cacccagaac tggttggaat cggggctcaa ggaccggccg atcacccggg
     3541 acatggcgtg gggggtcccg gtcccgctcc cgggcatgga gggcaaggtc atatacgtct
     3601 ggttcgaagc ggtcatcggc tatctctcgg ccagcaagga atgggccatg agcacggggg
     3661 acccggacgc ctggagggag ttctggaccg accccgaggt caagggatac tacttcctgg
     3721 ggaaggacaa catccccttc cataccatca tctggcccgc catcctgatg ggatacggag
     3781 ggctcaatct gccctacgat gtgccggcga acgagttcct caccttcaag ggggagaagc
     3841 tctcgaaaag caaggggccg agcatcgaca tccccagcat cctgaaggtg ttcgacgccg
     3901 accagatcag gtactatttg gcaagcaaca tgccggaggg aaaggacgcg gacttctcct
     3961 gggaggactt cgagaccaag gtcaacaacg aactcgtggc caccctggga aactatttcc
     4021 acagggtgct gagcttcacc cagaagaact tccaccaggt ccccccgctc ggcgaggggc
     4081 tggaggtgga gaccgaacag gtggagggcg ccatcgccca agccatcgcc gaggtggacg
     4141 ccaacatttc ctcctgccag ttcaagaggg ggttgaaggc cataatggac ctggcccagt
     4201 tcgggaaccg cttcttcgat tcctgcgccc cttggagcat gctgaggtca gaccgggaca
     4261 ggtgcggggg gatgctccac ttgaacctga gggtcgtcca ggccttggca gtgctcgccc
     4321 atccctatct tccgttcacc acccagaggc tctgggcgtt cctggccaac gacggggcca
     4381 tcggggaagg gtcctggagc gagatcacca agcccctgaa cgtcggccgc gaactccctg
     4441 agccaaggcc attgtaccgc aaggtggaca tccagcagga ggagcggccc ttctcagggt
     4501 tcgaggccct ggacctaagg gtaggggtgg ttaaggaggt cagcgatcac cccaacgccg
     4561 ataggctcat ggtcctcaag gtcgatatcg gtcggccgat acagttggtc gccggcctga
     4621 aggccttcta caccaaggag gagctcaccg ggaagaaggt agtggtgatc gccaatctcc
     4681 aacccgcgaa gctcaggggg gtggagtcac agggcatggt cctcgccgcc gaggccgacg
     4741 gcgaggtaaa ggtgctcacg ccatccgggc ccgcggagcc gggagatcgc gtgaccagcg
     4801 gcctcagccc ggggggcagg ccattgtcat tgcaggaatt ccaggccttc gtgctgagga
     4861 ccggcacggt gctcaacgac caggaggcag acgtcgggag gaggggtaag g
//
LOCUS       DAOSYN010000093         4870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_14443_length_4870_cov_4.357009, whole genome shotgun sequence.
ACCESSION   DAOSYN010000093 DAOSYN010000000
VERSION     DAOSYN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14443_length_4870_cov_4.357009"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLJ11_04820"
     CDS             complement(<1..362)
                     /locus_tag="PLJ11_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HOO04027.1"
                     /translation="MAATKKASTKAASRKVKDRWKAKEWYKIYAPRMFNQVQLGETPS
                     ADAANLIGRTTEATVHDINGDFSKMHVKLKFKVQDVSGFDAHTVFIGQDLASDYIRRL
                     TRRKRTKTDHVVDVRTKDG"
     gene            complement(480..764)
                     /locus_tag="PLJ11_04825"
     CDS             complement(480..764)
                     /locus_tag="PLJ11_04825"
                     /inference="COORDINATES: protein motif:HMM:NF017208.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF749 family protein"
                     /protein_id="HOO04028.1"
                     /translation="MHQAHLVGVYTQEEVPEEVEGFVRYQAARDGHRAVPGERIAVLN
                     VIGTSSYVPVFLMDVDSLAELESKLSEHGVQADQVSKASLRRVLQEMGRT"
     gene            complement(774..1628)
                     /gene="hdrB"
                     /locus_tag="PLJ11_04830"
     CDS             complement(774..1628)
                     /gene="hdrB"
                     /locus_tag="PLJ11_04830"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit B"
                     /protein_id="HOO04029.1"
                     /translation="MAADKFALFLGCVAPLRYPGIEKATRELFKALGYDLREMTGAGC
                     CPAPGVVRSFDQATWLALAARNLAIAEKQGCDVLTICNGCFGSLYEANHMLQEDPALL
                     KKVNAILAEVGMKYEGKVRVRHFADVLFNDVGIEKMKQKFTRPLDYKVAVHYGCHFTR
                     PTKLKHLDDPERPHILDELVEWTGAKSLPYADKNMCCRAGGGVRSRDGKFALTFTEAK
                     LKNMKAVNAQFIIDVCPFCHLQFDRGQKDLPGYDIPVIHLAQLYGLAMGLPESSLGLE
                     AHEIKVTL"
     gene            complement(1629..2138)
                     /gene="hdrC"
                     /locus_tag="PLJ11_04835"
     CDS             complement(1629..2138)
                     /gene="hdrC"
                     /locus_tag="PLJ11_04835"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HOO04030.1"
                     /translation="MSNVKIPDPDFAKSIIDAGGKTLDLCYQCGTCTGSCPSGRTTAF
                     RTRQLIRKAQLGLKDDILPSEDLWMCTTCYSCVERCPRDVEIVDIIILMRNMAVQKGY
                     LAEEHRKVGRSLLKIGHTVPASDKLKQLRVSMGLTADPPTTIADPKAMADFRKIMEKT
                     GFEKLIGGA"
     gene            complement(2227..>2724)
                     /locus_tag="PLJ11_04840"
     CDS             complement(2227..>2724)
                     /locus_tag="PLJ11_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04031.1"
                     /translation="TPLTEEMLLLALNAADPQGMAEMRISDDRRNVADLQAGLVDLVV
                     LDDPLYAFEMDDMMWEEVGSDRLFHVRKGEEYALYRFGPQRLGLRTLDSSNERYRVVR
                     TYSSLAPLLRSGYSYFVSEGLLLRKGHRPRGDEPSIPYAILCVYRPTVGTEAVLQALR
                     ANRRT"
     assembly_gap    2725..2734
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2735..2992)
                     /locus_tag="PLJ11_04845"
     CDS             complement(<2735..2992)
                     /locus_tag="PLJ11_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO04032.1"
                     /translation="MVQVRASLTVQVNGRPLTEKQAKAVLALYLNGTQRSAARSLGIS
                     APVLCRHLRQVEVKAGAPIMDCGARGTVLNELGETIAKEQLA"
     gene            3082..3543
                     /locus_tag="PLJ11_04850"
     CDS             3082..3543
                     /locus_tag="PLJ11_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOO04033.1"
                     /translation="MKASRLLLGIGSPIVCDDGLGFRVVEEVRAMGLPDLDVDQQSVS
                     GLDLIEIMMDYKRVVVVDAIVTGRYPAGTVMVLEPEDFKSALHGTNPHEVNIHMAIEL
                     GRRLSPERMPRDIRFVAMEVKDVWTVSDALTEDVERAVPLAVRTVLELLGD"
     gene            complement(3540..4817)
                     /locus_tag="PLJ11_04855"
     CDS             complement(3540..4817)
                     /locus_tag="PLJ11_04855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO04034.1"
                     /translation="MAESGPGLHRTTAGVPVIVEEIPRSRTAALSVNFKVGCRDEDQD
                     LCGTAHFLEHIMFKGTHKRNARQFSELVEGAGGEMNAYTTKESTSFHVFSLDETFDVM
                     KGLMADMMTAPLIDEEHVELERGVVLQEISMLEDDPEDYSRVLLDRTIWEGHPMANTE
                     TGRPEWVKELKARDLRRFFEDHYRPPNMYIVASGHVSAEDVVSWTEGTFDHFPPATRS
                     LKRVPPEPKACTKVFPREGDQAYVELGFPGYPGDHPDRQALLVACIILGGGTSSRLYQ
                     TVREDQGLVYHIGTFPQSYTDCGIVDTFFSASIGKLDRVMRTLAKELAAFKDQGPTEE
                     EVRRAKRWVKGMLVRKLEGTDSRLYWLGEEYATCGKVADAEASIREFEAVRREDVARA
                     MNDVFQRKRMCAVLLAPEKAGEATARRMQSLDF"
BASE COUNT          816 a         1522 c         1539 g          983 t
ORIGIN      
        1 ccgtccttgg tgcggacgtc cacgacgtga tcggtcttgg tccgcttgcg gcgcgtcaaa
       61 cgccttatgt aatcgctggc caggtcttgg ccgatgaaca cggtgtgcgc atcgaacccg
      121 ctcacgtcct ggaccttgaa cttgagcttg acgtgcatct tcgagaagtc gccgttgatg
      181 tcatgaacgg ttgcctcggt ggtcctgccg atcaggttag cggcatcggc gctgggggtt
      241 tcgcccagct ggacctgatt gaacatgcgg ggagcgtaga tcttgtacca ctccttggcc
      301 ttccaccggt ccttgacctt ccttgaggcg gccttcgtag acgctttctt agtagcagcc
      361 aattaatagc ctccaatcgc gagatgaact cggaaacaag taaccctatt aataagtttt
      421 ctccttttcc gtgcggtcgt ccaattttca ctggaaaggg aaaaaccgag ggaaagggtt
      481 taggtgcgcc ccatctcctg tagcacccgg cgcagggacg ccttgctcac ctggtccgcc
      541 tggaccccat gctcggagag cttggattcc agctccgcaa ggctgtccac gtccatgagg
      601 aagacaggga cgtaggagga ggtaccgatg acgttgagca cggctatcct ctcccccggc
      661 accgctcggt gcccgtcccg ggcggcctgg tacctgacga agccctccac ctcttcaggc
      721 acctcctcct gggtgtagac ccctaccaga tgcgcttggt gcaagcccat gcctcacagg
      781 gtgaccttga tctcgtgggc ctcgagcccc agggaggact cagggaggcc catggccagg
      841 ccatagagct gcgccaggtg gatgaccggt atgtcgtagc cgggcaggtc cttctggccg
      901 cggtcgaact gcagatggca gaacgggcac acgtcgatta tgaactgggc gttgacggcc
      961 ttcatgttct tgagcttggc ctcggtgaag gtcagggcga acttgccgtc cctgctgcgc
     1021 acgccccctc cggcccggca gcacatgttc ttgtcggcgt acgggaggct cttggcgccc
     1081 gtccactcca ccagctcgtc caggatgtgc ggcctctcgg gatcgtccag gtgcttgagc
     1141 ttggtgggcc tggtgaagtg gcatccgtag tgcacggcca ccttgtagtc cagcggccgg
     1201 gtgaacttct gcttcatctt ctcgatgccc acatcgttga acaggacatc ggcgaagtgc
     1261 cggacgcgga ccttgccctc atatttcatg ccgacctcgg ccaggatggc gttgaccttc
     1321 ttcagtaggg cggggtcctc ctgcagcatg tggttcgctt cgtacagaga gccgaagcag
     1381 ccgttgcaga tggtgaggac gtcgcatccc tgcttctcgg cgatggccag gttgcgggcg
     1441 gcgagcgcca gccaggtggc ctggtcgaag gaacggacca ccccgggggc ggggcagcac
     1501 ccggcaccgg tcatctccct caggtcgtag ccgagggcct tgaacagctc cctggtcgcc
     1561 ttctctatgc caggatatct gaggggggcg acgcacccca ggaacagagc gaacttgtca
     1621 gcggccattt acgcccctcc gatcagcttc tcgaacccgg tcttctccat gatcttcctg
     1681 aagtcggcca tggccttcgg gtcggcgatg gtggtcggcg gatcggcggt aaggcccatg
     1741 ctgacgcgca gctgcttgag cttgtccgag gcgggaacgg tgtgcccgat cttgaggagg
     1801 gagcgcccga ccttcctgtg ctcctcggcc aggtagccct tttggaccgc catgttcctc
     1861 atcaggatga tgatgtccac gatctcgacg tcccgggggc acctttccac gcatgaatag
     1921 caggtggtgc acatccagag gtcctcggac ggcagtatgt cgtccttcag tcctagctgg
     1981 gccttcctga tcagctgccg ggtgcggaac gcggtggtcc gtccggaggg gcagctgccg
     2041 gtgcatgtgc cgcactggta gcacaggtcc aaggtcttgc cgccagcatc gatgatgctc
     2101 ttggcgaagt ccgggtctgg tatcttaacg ttcgacatgg aagtattcct cttagggcgg
     2161 ggagagggga cgccctatat aaagatggac gagaaaaggc tccgagtgtt gtctatatag
     2221 gttacattat gtccgccgat tcgcccgaag ggcctgcagg accgcctccg tccctaccgt
     2281 gggccggtag acgcagagga tggcgtacgg gatggagggc tcatcgcccc tgggcctatg
     2341 ccctttcctg agcagcaggc cttcgctcac gaaatagctg tacccgctgc ggagcaaggg
     2401 ggccaaggag gagtacgtcc gtaccaccct gtacctttca ttggaggagt cgagggtgcg
     2461 gagccctagg cgctggggcc cgaaccgata gagggcgtac tcctccccct tgcggacgtg
     2521 gaaaagccgg tcgctgccca cctcctccca catcatgtca tccatctcaa aggcgtacag
     2581 gggatcgtcc agcaccacta ggtcgacaag cccggcctgg aggtcggcca cgttgcgcct
     2641 gtcgtccgat atcctcatct ccgccatgcc ttgcgggtcg gcggcgttga gggccaacag
     2701 gagcatctcc tcggtcaggg gggtnnnnnn nnnncgccag ctgctccttc gctatcgtct
     2761 ccccgagctc gttcagcacc gtcccgcggg ccccgcagtc catgatgggg gcgccggcct
     2821 tgacctccac ctgtcggagg tgcctgcaga ggacgggggc ggatatcccc aaggagcggg
     2881 cggcagaccg ctgggtgcca ttgagataga gcgccaggac cgccttggcc tgcttctccg
     2941 tgagcgggcg gccgttgacc tggacggtga gcgaggctcg cacctggacc atggccctgc
     3001 atgcccgtgg ggctagatta atccgccgac cggagcgggc ccggggcaaa gttaatagcc
     3061 tgcgcctgct tccgcggcat gatgaaggcg tcccgtctgt tgctgggcat cggcagccct
     3121 atcgtttgcg acgatggcct cgggttccgg gtggtggagg aggtgagggc gatgggcctg
     3181 ccggaccttg acgtggacca gcagtcggtc agcggcctcg acctcatcga gataatgatg
     3241 gactataaga gggtggtcgt ggtggacgcc atcgtcaccg gtcgataccc tgccggcacg
     3301 gtcatggtcc tggagcccga ggacttcaag agcgccctgc acgggaccaa cccgcacgag
     3361 gtcaacatcc acatggccat tgagctggga aggcgccttt cgccggagcg catgccccgg
     3421 gacattaggt tcgtggccat ggaggtcaag gacgtgtgga ccgtctcgga tgccttgacc
     3481 gaggatgtgg agagggccgt ccctctggcc gtgcggaccg tgctcgagct tcttggcgat
     3541 tagaaatcca gggactgcat gcgcctggcg gtcgcctccc ccgctttctc cggggctagc
     3601 aggacggcgc acatcctctt cctctggaag acatcgttca tggctcgggc cacatcctcc
     3661 cgtctcaccg cttcgaactc gcggatggat gcctcggcgt ccgcgacctt gccgcacgtg
     3721 gcatactcct cccccagcca gtaaagccgg ctgtccgtgc cctcgagctt tctgaccagc
     3781 atgcccttga cccaccgctt ggccctccgc acctcctcct cggtggggcc ctggtccttg
     3841 aaggcggcca gctccttggc cagggtcctc atcacccggt ccagcttgcc gatggaggcg
     3901 gagaagaatg tgtcaacgat cccgcagtcg gtgtacgatt gggggaacgt gcctatatga
     3961 tatacgagcc cctggtcctc acggacggtc tggtacagtc gggaggaggt ccccccgccc
     4021 aatattatgc aggcgacgag gagcgcctgc cggtctggat gatcgcctgg atagcctggg
     4081 aagccaagct ccacataggc ctggtccccc tccctgggga agaccttggt gcaggccttc
     4141 ggctccggag ggaccctttt cagggagcgg gtggccgggg gaaaatggtc gaaggttccc
     4201 tccgtccaag agaccacgtc ctcggccgaa acgtgcccgg aggccacgat gtacatgttc
     4261 ggagggcggt agtggtcctc gaagaagcgc cggaggtccc tggccttcag ctccttgacc
     4321 cattccgggc gaccggtctc ggtattggcc atcggatggc cctcccagat ggtgcggtcc
     4381 aggagcaccc tggagtagtc ctccgggtcg tcctcgagca tgctgatctc ctgcagcacc
     4441 acccctcgct cgagctccac gtgctcctcg tcgatcagag gcgcggtcat catatcggcc
     4501 atgagcccct tcatcacgtc gaacgtctca tccagcgaga acacgtggaa cgacgtgctc
     4561 tcctttgtgg tataggcgtt catctccccg ccggcgccct ccacgagctc cgagaactgc
     4621 cgggcgttcc ttttgtgggt ccccttgaac atgatgtgtt cgaggaaatg ggccgtgccg
     4681 cagaggtctt ggtcctcgtc ccggcatccc accttgaagt tgaccgaaag ggctgcggtg
     4741 cgtgagcggg ggatctcctc cacgatcacc ggcacgccag cagttgtacg gtgcagccct
     4801 ggtccagatt cagccacagc cctggaacgc tttccctgga taatggtttt ggggtgcggg
     4861 caccgtcaac
//
LOCUS       DAOSYN010000094         4781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_14758_length_4781_cov_4.116589, whole genome shotgun sequence.
ACCESSION   DAOSYN010000094 DAOSYN010000000
VERSION     DAOSYN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14758_length_4781_cov_4.116589"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLJ11_04860"
     CDS             complement(<1..611)
                     /locus_tag="PLJ11_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOO04035.1"
                     /translation="MVFKLDLSGLRHGTELVKRGFAKMQQGGVIMDVTNAEQAVIAEE
                     AGAVAVMALERVPADIRAEGGVARMADPVKILEIMDAVTIPVMAKCRIGHFVEAQVLE
                     AIGVDMIDESEVLTPADPFMHVAKQYFTVPFVCGARDLGEALRRIDEGAAMIRTKGEP
                     GTGNIIEAVRHQRIIMGKVRELKGKDEAELAVIARQIEAPFYL"
     gene            733..1887
                     /locus_tag="PLJ11_04865"
     CDS             733..1887
                     /locus_tag="PLJ11_04865"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOO04036.1"
                     /translation="MFAKRMENVLESGTVKTANLVSKLKGEGADIISFSMGEPDFHTP
                     ENIKEACMRSLANNFTYYTPSAGIPELRRAIADRCRRLNNIPCDASNVLVTPTKQAIF
                     MTMLAMVDEGDEVILPDIAWGTFEACARLAGGKVRYAHLSVENAFRMTPEAVAEKITK
                     RTKLLVLNSPSNPCGAVLTKGDVKGIADLAQDHGFFVLADEIYNQLVFEGEHVSISSL
                     EGMFDRTITVDGFSKTYAMTGWRAGWAVAPKPIFKELNKLQTQSITCVTSFVQQACLE
                     ALHGPQGSVAMMKQEFKARRELVYALMKDIPSLDCPMPSGAFYMMPSYAFDMSSEDMA
                     AYLLEEAHVAVTPGSAFGPAGEGRFRLSYAASRENIEKGMARIKDALARL"
     gene            complement(1888..2064)
                     /locus_tag="PLJ11_04870"
     CDS             complement(1888..2064)
                     /locus_tag="PLJ11_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HOO04037.1"
                     /translation="MKRELMDILACPVCKHHPLLLEVEREMGGEVMEGRLLCQRCKAV
                     YPIEDGIPDMLPPE"
     gene            2165..3199
                     /locus_tag="PLJ11_04875"
     CDS             2165..3199
                     /locus_tag="PLJ11_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOO04038.1"
                     /translation="MRRIDLRSDTFTLPSKEMLDSIPSAELGDDVEGEDPTVNQLQDM
                     AADMFGAEAALLVASGTMGNLVSLMAHCRRGDEVFLEAEAHIYYYELGGMSAIVGAIP
                     NLIKGVRGKFTSEQLEAAIRHPDLHYPIPRLVCIENTHNRGGGTVWTPREVEAVAKVA
                     HDHGLKVHCDGARIFNAAVALDVDVKEYMRHLDSLSFCLSKGLACPVGSVVLGNREFI
                     EEVRRARKMVGGGMRQAGIIAAPGIIALQKMVGRLKEDHRNARRLAEGLNQIPGVKVD
                     LDSVQTNIVLADITGTGWDVDYFVNRAGKIGVQVFEFGATKARFVTHYGIDEQDIDEV
                     LSRLRRLLQR"
     gene            3326..4390
                     /locus_tag="PLJ11_04880"
     CDS             3326..4390
                     /locus_tag="PLJ11_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOO04039.1"
                     /translation="MSRRLFTVGPVEVFPDTLASMNRAMIVHRGKEYQDLQHRVVEKI
                     RKVLDIDSDVLLAPASASGFLEGCVRSAVETKMAGLSNGSFGDRWQQIGQENGKQVQR
                     LNGEWGKALRAPHIAGKLERDVEAVTLVSNESSTGVLNPVGEIVNAIREQSDALVMVD
                     GVTAVGAVDLKLRKLDLDALVFGSQKALALPPGLAVVCVSDRLMERAAKVKNRGYYLD
                     LVQYKKMADKDLPLTTPPVSLMYGLDYQLDKILREGVQERYARHQRMADMVRGWASKR
                     LGLYAEEGFRSNTITVIKLNGLDFKEFDRRLRQKGFEVSPGYGTVKDVTFRIGHMGDL
                     SERDIQDLLDAMDETMEEMR"
     gene            4387..>4781
                     /locus_tag="PLJ11_04885"
     CDS             4387..>4781
                     /locus_tag="PLJ11_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOO04040.1"
                     /translation="MKVLVTDELSQEGLDMLTKGSDIQVDVRPKLAHEELLKIIGEYD
                     ALIVRSGTKVTAEVIEAGKRLRVIGRAGVGVDNVDVEAATRRGVLVMNTPAANIISAA
                     EHTMAMMLTLARNIPRADASVKAGKWERS"
BASE COUNT          944 a         1492 c         1533 g          812 t
ORIGIN      
        1 atgaggtaga agggggcctc tatctggcgg gcgattaccg ccagctccgc ctcgtccttg
       61 cccttcagct cccgcacctt ccccattatg atcctctgat gccgcacggc ctcgattatg
      121 ttccccgttc caggctcgcc cttggtcctg atcatggccg ccccctcgtc gatgcgccgc
      181 aatgcctcgc ccaggtccct cgccccgcag acgaagggta cggtgaaata ctgcttggcc
      241 acgtgcatga acggatccgc gggggtaagc acctccgact cgtcgatcat gtccacgccg
      301 atggcctcca gcacctgtgc ttccacgaag tgcccgatgc ggcatttggc catgaccggg
      361 atcgtcaccg cgtccattat ctccaagatc tttacggggt cggccatgcg ggccacccct
      421 ccctcggccc tgatgtccgc cgggaccctc tccaaggcca tgacggccac tgcgcccgcc
      481 tcctcggcga tgacggcctg ctcggcgttg gtcacgtcca tgatcactcc tccctgctgc
      541 atcttcgcga acccgcgctt gaccagctcg gtgccatggc gcagtccgga caggtccaac
      601 ttgaatacca tattgctcac cagatcggct tacctgcaga gctttattta tcacttttcc
      661 caacgcattc cagctatacc gacagaccta cctgagaaaa gcataaagac atcgtgctcg
      721 ttcaacagct cgatgttcgc gaaaaggatg gagaacgtcc tggagtctgg tacggtcaag
      781 acggccaacc tggtcagcaa gctcaagggg gagggggcgg acatcatctc gttctccatg
      841 ggggagcccg atttccacac gcctgagaac attaaggagg cgtgcatgcg ctccttggcc
      901 aacaacttca cctattacac cccgtccgca gggatcccgg agctccgcag ggccatcgcg
      961 gaccggtgca ggaggctcaa caacatccca tgcgacgcct ccaacgtcct ggtcaccccg
     1021 accaagcagg ccatcttcat gaccatgctg gccatggtgg acgaggggga tgaggtcatc
     1081 ctgcccgaca tcgcgtgggg gacctttgag gcatgcgccc gcctggccgg cgggaaggtc
     1141 cggtatgccc atctctccgt ggaaaacgcc ttccgcatga cccccgaggc ggtggcggag
     1201 aagatcacga agaggaccaa gctgctggtc ctcaactcgc cctccaaccc ctgcggggct
     1261 gtgctgacga agggggacgt caagggcatc gccgacctcg cccaggacca cggcttcttc
     1321 gtcctcgccg acgagatata caatcaactg gtgttcgagg gggagcacgt ctccatctcc
     1381 tcgctggagg gcatgttcga caggaccatc accgtggacg ggttctcgaa gacctacgcc
     1441 atgacgggat ggcgggcggg ctgggccgtc gcgccgaagc ccatcttcaa ggagctcaac
     1501 aagctccaga cccagtccat cacatgcgtc acctccttcg tgcagcaggc ctgcctggag
     1561 gcgctgcatg gaccccaggg gtccgtggcg atgatgaagc aggagttcaa ggcccgccgg
     1621 gagctggtgt acgcgctgat gaaggacatc cccagcctgg actgcccaat gccgagcggg
     1681 gcgttctaca tgatgccctc ctacgccttc gacatgagct ccgaggacat ggccgcctac
     1741 ctgctagagg aggcgcatgt ggccgtcacg cccgggtccg ccttcgggcc ggcgggggag
     1801 ggcaggttcc gcctgtcgta cgccgccagc agagagaaca tagagaaggg catggccagg
     1861 ataaaggacg ccttggccag gctctgatca ttccgggggg agcatgtccg ggatgccgtc
     1921 ctcgatgggg tagacggcct tgcaccgctg gcagaggagc cggccctcca tcacctcccc
     1981 gcccatctcc cgttccacct ccagcaggag gggatgatgt ttgcacaccg ggcacgccag
     2041 gatgtccatg agctccctct tcatgccaaa ccccaggcgc tagcccgcat atcaaccttt
     2101 cagcggcgtc agggcgctaa ctatttatcc ggtcctccgc atcgagcggc ctggagagat
     2161 gcgattgcgc cggatagacc ttaggagcga taccttcacc ctgccttcca aagagatgct
     2221 ggacagcata ccgtccgccg aactgggcga tgacgtggag ggggaggacc ccacggtgaa
     2281 ccagctgcag gacatggcgg ccgacatgtt cggggccgag gccgcgctgc tggtcgcaag
     2341 cgggacaatg ggcaacctcg tgtccctgat ggcccattgc cggaggggcg acgaggtctt
     2401 cctggaggcc gaggcgcaca tctactatta cgagctcggg ggaatgtccg cgatcgtcgg
     2461 agcgatcccc aatctgatca agggggtgcg cgggaagttc acctccgagc agcttgaggc
     2521 ggccatccgc catccggacc tccattaccc catcccccgc ctcgtttgta tagagaacac
     2581 gcacaaccgg gggggaggga cggtctggac acccagggag gtcgaggcag tggccaaggt
     2641 ggcccacgac catggcctga aggtccattg cgatggcgcc cgaatattca acgccgccgt
     2701 ggcgttggat gtggacgtca aggagtacat gcgacacctg gacagcctga gcttctgcct
     2761 gtccaagggg ctggcgtgcc ctgtgggatc ggtggtctta gggaacaggg aattcatcga
     2821 ggaggtccgc cgcgcccgca agatggtcgg tgggggcatg cgccaggccg gcatcatcgc
     2881 cgcccctggg atcatagcgc tgcagaagat ggtcggccgg ctgaaggagg accaccggaa
     2941 cgcccgcagg ctggcggaag gcctaaatca gattccgggc gtcaaggtcg acctggactc
     3001 ggtgcagacc aacatcgtgc tggcggacat caccggcacc ggatgggacg tggactactt
     3061 cgtgaacagg gccgggaaga tcggggtcca ggtgttcgag ttcggcgcca ccaaggccag
     3121 gttcgtcacc cattacggca tcgacgagca ggacatcgat gaggtccttt cccgcctccg
     3181 ccgcctgttg caacggtagg ccatggaaac ctatttcatt cggggccctc taccctctca
     3241 ggccgcagtg ccaagggccg caaggccctc cggcccaacg gtatgaggga aagcgagcga
     3301 cgcgcattca cggtgaggga tcgatttgag cagaaggctt ttcacggtcg gaccggtcga
     3361 ggtgttcccg gacacactgg cctcgatgaa cagggcgatg atcgtccacc ggggcaagga
     3421 ataccaggac ctgcagcaca gggtggtcga gaagattcgc aaggtacttg acatagacag
     3481 cgacgtcctc ctcgcccccg cctccgcctc cggcttcctg gagggctgcg tgcgcagcgc
     3541 cgtggagacc aagatggcgg ggctttcgaa cgggtccttt ggggaccgct ggcagcagat
     3601 cggccaggag aacgggaagc aggtgcagcg gctcaacggg gaatggggca aagccctacg
     3661 cgcgccgcac atcgctggaa aactggaaag ggacgtggag gcggtgacgc tcgtttccaa
     3721 cgaatcgtcc accggggtcc tgaacccggt gggggagatc gttaatgcga tcagggagca
     3781 gagcgacgcc ctggtcatgg tggacggcgt gaccgccgtc ggcgcggtcg acctcaagct
     3841 ccgcaagctg gacctggacg cccttgtctt cggcagccaa aaggccttgg cccttccacc
     3901 cgggctggcg gtggtctgcg tctcggaccg cctgatggag agggcggcca aggtcaagaa
     3961 ccggggctac tacctggacc tagtgcagta caagaagatg gcggataagg acctgcccct
     4021 gacgacgccc cccgtgtccc tcatgtacgg gctggactat cagctggaca agatcctcag
     4081 ggagggcgtg caggagaggt acgcccggca ccagcgcatg gcggacatgg tgaggggatg
     4141 ggcctccaag cgcctaggcc tctatgccga ggaaggcttc cgctccaata ccatcaccgt
     4201 gatcaagctc aacggcctcg acttcaagga gttcgaccgg cgcctccggc aaaagggctt
     4261 tgaggtctcc cccggatatg ggaccgtgaa ggacgtgaca ttccgcatcg gccacatggg
     4321 agatctgagc gagagggaca tccaggacct cctggatgcc atggacgaaa cgatggagga
     4381 gatgagatga aggtcttggt aaccgacgaa ctgtcccagg aaggcctgga catgctgacc
     4441 aaggggtccg acatccaggt ggatgtgcgg ccgaagctgg cgcacgaaga gctgctcaag
     4501 ataatcggcg agtacgacgc gctcatagtg cgcagcggca ccaaggtcac cgcggaggtg
     4561 atcgaggccg ggaagagatt gcgcgtgatc ggaagggccg gcgtgggggt ggacaacgtg
     4621 gacgtggagg cggccacgcg gcgcggcgtc ctggtcatga acaccccggc cgccaatatc
     4681 atctccgcgg cggagcacac catggccatg atgctcacct tggccaggaa catcccccgt
     4741 gcggatgcct cggtgaaggc cggtaagtgg gagaggagca a
//
LOCUS       DAOSYN010000095         4768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_14815_length_4768_cov_4.532782, whole genome shotgun sequence.
ACCESSION   DAOSYN010000095 DAOSYN010000000
VERSION     DAOSYN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14815_length_4768_cov_4.532782"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /gene="infB"
                     /locus_tag="PLJ11_04890"
     CDS             <1..1336
                     /gene="infB"
                     /locus_tag="PLJ11_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOO04041.1"
                     /translation="SPATPFILSEKTQSEEARAALADKLYAIVGRLSEEGLSADRYDR
                     IENFTKNIALVPISARNGEGVPDLLLVLIGLAQRFLEDSLRTAEGPAKGTILEVKEER
                     GLGQTIDVILYDGELQQGDQVALGTKGRPLITKVKAILKPKPLDEIRDPRDKFDRVKT
                     VGAAAGVKLLCQNLDGAVAGAPLRAIKGNLEEVLRDIAEETTIDIEVADDGVMIKADA
                     LGSLEALAYECKRAGIPIRKYEIGDISKRDIIEVSSYTDPLHKVVLGFNVSMLPDAKD
                     ALTTHQSKVFLNAVVYGLIDDYLKWVEEEKRRAEEEKRALTAFPGKIRVLPDCVFRAS
                     KPAIVGVRVLAGRIRTNQRLISADGKEIGRIRSLRTGEDALKEATAGQEIAVAIEGAT
                     VGRQLNVDDILYVDIPESEVKGLQDYDLNLDEREVLEELMAIKRREDPFWGM"
     gene            complement(1337..2005)
                     /locus_tag="PLJ11_04895"
     CDS             complement(1337..2005)
                     /locus_tag="PLJ11_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO04042.1"
                     /translation="MLRSAVPMVALVMLLAALAPGSAAAVSEDGTVTVAAGGHAVLLD
                     FTVAGTGVDDPAAAGNVRYLVYLDEGSEQDVFDVFVMPYESYQGYLAGEGFRPVSGWS
                     SEGAGAAPAYNLVYLFDEGRYVLLIDNSDVGGAPKAPAELKVRYQLEANNVEVEREPR
                     WGAFLGLLALVALLGGAFLVGLSLYLKRRVGHVDAQRRKRCASCDKVSITDGRFCPYC
                     GKER"
     gene            2078..3640
                     /locus_tag="PLJ11_04900"
     CDS             2078..3640
                     /locus_tag="PLJ11_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOO04043.1"
                     /translation="MSRTVIRKHNPATLEVIGEVEATIPEEVPSIVERARTAQMGWSA
                     LPVKERERILRQVQAALCADMDALIDVVCRETGKPRLEALATDGLGALGAADFAIGRM
                     ARLFQESRVDLGGLSAAMRYMGRTSLLIPRPLGVIGIIAPWNYPLAIPYSQTMMALAA
                     GNAVVLKPSSQTPFTALRMGEVMAKAGLPEGLLQVVLGPGGAVGEALVTSGVDRIIFT
                     GHPDVARRVMVLASQRLTPMTLELGGKDALIVLEDADLRRAARAACWGAFVNCGQTCV
                     AVKRIYVHRAIEERFTELLLREVSAIRQGYDPRDPTITLGPLISEEAVQDMEAQVARA
                     VEQGAVVLVGGARPPGLKGHFFQPTVLSGAEQGADIVQRETFGPVVVLLPFEGDEEAV
                     RLANDSKYALNGSVWTADLARGKALATRMRSGTVTVNNVAYTYGLGATPWGGRGESGF
                     GRTHGDAGLEELIERQHVHLDRGRFPSEVWWPPYGEDGVEAVHTLIGMAFTGERAHLM
                     RGLLQVRRLMRR"
     gene            3691..4353
                     /locus_tag="PLJ11_04905"
     CDS             3691..4353
                     /locus_tag="PLJ11_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04044.1"
                     /translation="MRSRGWNRRAKFYFALTIALIVAAWTYTWFTREVPLHHELLIPF
                     AFVTLGMAIKYGDQVFDLNIGSKRKAVLLSIPVGALMGALIFLDEGSATIFIGLLLAL
                     LLVAKYDNLAFKLGFLTAGAMAVLAVLEGRPFHLLGTIIVMVAAIADEVLSDRGDRMR
                     EGALSLLLKERLVLKGAVLVLCAVGFLPTFLYFFAFLGFDTGYSFVEQISLSGGIGHR
                     KA"
     gene            4355..>4768
                     /locus_tag="PLJ11_04910"
     CDS             4355..>4768
                     /locus_tag="PLJ11_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04045.1"
                     /translation="MLVIGSGPAGAAAAFFIPYLSEGRLSVLSLEKLDRNFAKYHRMC
                     GEAVSRAAFDTLAPLEPHAVEFTIRRAVEHWPGDVTIETEAQGYVLDRPRFLTHVMDM
                     ARKEGCHTEVGAAVEVGRDENGYMVRTRDGRRFGAR"
BASE COUNT          845 a         1566 c         1612 g          745 t
ORIGIN      
        1 ctctcccgcg acccctttca tcctctccga gaagacccag agcgaggagg ccagggccgc
       61 gttggccgac aagctctacg ccatcgtcgg gcgtctctcc gaggaaggcc tctccgcgga
      121 tcgctacgac cgcatagaga acttcaccaa gaacatcgcc ctggtgccca tcagcgcccg
      181 caatggcgag ggcgttccgg acctgctgct ggtgctgatc gggctggctc agcgcttcct
      241 cgaggattcc ctgcggaccg ctgagggtcc ggccaagggc accatcctgg aggtcaagga
      301 ggagcgcggg ctcgggcaga ccatcgacgt catcctgtac gatggggagc tgcagcaggg
      361 ggaccaggtc gccttgggga cgaagggcag gccgctcatc accaaggtca aggccatcct
      421 gaagcccaag cccctcgacg agatccgcga cccgcgggac aagttcgacc gcgtgaagac
      481 ggtgggcgcc gccgcgggcg tgaagctcct ctgccaaaac ctggacgggg cggtcgccgg
      541 cgccccgctt agagccatca agggcaatct ggaggaggtg ctgagggaca tagcggagga
      601 gaccacgatc gacatcgagg tcgccgacga cggggtgatg atcaaggccg acgccctggg
      661 ctccctcgag gccctggcgt acgagtgcaa gagggccggg atccccatcc ggaagtacga
      721 gatcggggac atctccaagc gggatatcat cgaggtcagt tcgtacaccg acccactcca
      781 taaagtggtc ctaggattca acgtcagcat gcttccggac gccaaggacg ccctcaccac
      841 ccatcaatcg aaggtgttcc tgaacgccgt ggtgtacggc ctgatcgacg actacctgaa
      901 atgggtggag gaggagaagc gccgcgccga ggaggagaag cgcgccctca cggccttccc
      961 gggaaagatc cgggtcctgc cggactgcgt tttccgggcg agcaagccgg ccatagtcgg
     1021 cgtccgggtg ctcgccggcc ggatccgcac caaccagcgg ctcatctccg cggatgggaa
     1081 ggagataggc cggatccggt cgctgcgcac gggcgaggac gccttgaagg aggcgaccgc
     1141 cggccaggag atcgccgtgg ccatagaagg ggcgacggtc ggacgccagc tgaacgtgga
     1201 cgacatcctc tacgtggaca tcccggaatc ggaggtgaag ggcctgcagg actacgacct
     1261 caacctggac gagcgggagg tcctggagga gctcatggcg ataaagcgga gggaggaccc
     1321 cttctggggc atgtgatcat cgctccttcc cgcagtacgg gcagaaccgc ccgtcggtta
     1381 tggagacctt gtcacagctg gcgcaccgtt tcctgcgctg cgcatcgaca tgcccgaccc
     1441 tgcgcttgag gtataggctc agccccacca ggaacgcccc gccgagcagc gcgaccaggg
     1501 ccaagaggcc caggaaggcg ccccaccggg gctccctctc cacctccacg ttgttggcct
     1561 cgagctggta acgcaccttc agctcggccg gggctttggg cgccccgccc acgtccgagt
     1621 tgtcaatgag cagcacgtac ctcccttcat caaagagata gaccaggttg tacgccgggg
     1681 ccgcgccggc gccttcggag ctccagccgc tgaccggtcg gaacccctcc ccggcaagat
     1741 acccctgata gctctcatag ggcatcacga agacgtcgaa cacgtcctgc tccgacccct
     1801 cgtccaaata gaccaggtag cgcacgttcc ccgcggccgc cggatcatcg acgccggtcc
     1861 ccgcgacggt gaagtccaac aggaccgcgt gcccccccgc cgccaccgtc acagttccgt
     1921 cctcggacac tgccgccgcg cttccagggg ccagcgcagc caggagcatc acgagcgcga
     1981 ccatcgggac ggccgacctg agcattgcca agggaatcgg cctccggcta gataaacaat
     2041 ggtcagcttc atctacgctc attgcgttcc caggacgatg agccgaaccg tcatccgcaa
     2101 gcacaacccc gccaccctgg aggtgatcgg ggaggtcgag gccaccatcc ccgaggaggt
     2161 cccctcgatc gtcgaacggg ccaggacggc gcagatgggc tggagcgctc tccccgtgaa
     2221 ggaaagggag cgcatcctgc gccaggtgca ggccgccctc tgcgcggata tggacgctct
     2281 gatcgacgtc gtatgcaggg agacggggaa gccgcgcttg gaggccctgg ccaccgatgg
     2341 cctgggcgcc ctcggcgctg ccgatttcgc catcggccgc atggcccggc tgttccagga
     2401 gagcagggtg gacctcggcg ggctctctgc ggcgatgagg tacatgggcc gcacctccct
     2461 cctcatcccc cgccccctgg gggtcatagg catcatcgcc ccctggaact atcccctcgc
     2521 catcccgtac tcgcagacca tgatggccct cgccgccggc aacgccgtgg tgctgaagcc
     2581 ttcctcccag acccccttca ccgccctgcg catgggggag gtcatggcca aggccggcct
     2641 gcccgaaggt ctcctccagg tcgtgctcgg ccccggaggg gcggtcggcg aggccctggt
     2701 gacctccggg gtggaccgca tcatcttcac cggtcacccg gacgtcgccc gcagggtgat
     2761 ggtgctagca tcccagcggt tgacccccat gaccctggag ctcggcggga aggacgccct
     2821 catcgtgctc gaggacgccg atctgcggcg ggcagcgagg gccgcctgct ggggagcgtt
     2881 cgtgaactgc ggccagacat gcgtcgcggt caagcgcatc tacgttcaca gggccatcga
     2941 ggaaaggttc accgagctgt tgctgcggga ggtctcggcc atccggcagg ggtacgaccc
     3001 acgggacccg acaataaccc tcggaccgct gatctccgag gaggcggtcc aggacatgga
     3061 ggcccaggtg gccagggccg tggagcaggg ggcggtggtc cttgtcggag gggcccggcc
     3121 cccggggctc aaggggcact tcttccagcc cacggtgctc tccggcgccg agcagggggc
     3181 cgacatcgtg cagagggaga ccttcggacc ggtggtggtc ctgctgccgt tcgaaggtga
     3241 cgaggaggcc gtccgcctgg cgaacgattc caagtacgcc ttgaacggaa gcgtatggac
     3301 cgccgacctg gcaaggggga aggcgttggc gacgaggatg cgctccggga cggtcaccgt
     3361 gaacaacgtc gcctacacct acgggctggg ggccaccccc tggggcggcc ggggggagag
     3421 cggcttcggg cgcacccacg gggacgccgg gctcgaggag ctgatcgaga ggcagcacgt
     3481 gcacttggac aggggccggt tcccctcgga ggtctggtgg ccgccctatg gggaggacgg
     3541 cgtggaggcc gtccacaccc tcatcggcat ggccttcacc ggggaacgcg cacatctcat
     3601 gcgaggactg ctccaggtaa ggcggctcat gcggcgttga gcgcatttat ataatcggtg
     3661 ccctttgaag agaggaggtc aggaggccac atgaggagca ggggatggaa ccgcagggcc
     3721 aagttctact tcgccctgac catcgccctg atcgttgcgg catggaccta cacctggttc
     3781 acccgggagg tccctctgca ccatgagctc ctcatcccct tcgcgttcgt cacgttgggg
     3841 atggcgataa aatacgggga ccaggtcttc gacctgaaca tcgggagcaa gcgcaaggcg
     3901 gtcctgctct ccatccccgt gggggcgctc atgggcgcat tgatattcct ggacgagggc
     3961 tctgccacca tcttcatcgg gctgctgctg gccctgctgc tggtggccaa gtacgacaac
     4021 ctggcgttca agctgggctt cctgaccgcc ggcgcgatgg ccgtcctggc ggtgctcgag
     4081 gggaggccct tccacctgct cgggaccatc atcgtcatgg tcgccgccat cgccgacgag
     4141 gtgctaagcg accgcgggga ccggatgagg gagggagcgc tatccctgct cctgaaggaa
     4201 cggctggtgc tcaagggggc cgttctggtc ctgtgcgcgg tcggcttcct gcccaccttc
     4261 ctctacttct tcgccttcct cggcttcgac accggctact ccttcgtgga gcagatcagc
     4321 ctctcagggg ggatcggcca ccgaaaggca tgacgtgctg gtgatcggct ccggcccggc
     4381 cggggccgcc gcggcgttct tcattcccta cctgagcgag ggaaggctca gcgtcctctc
     4441 tctagagaag cttgacagga acttcgccaa gtaccatcgg atgtgcgggg aggcggtgag
     4501 cagggccgcc ttcgataccc tcgcccccct ggaaccgcac gcggtggagt tcaccatccg
     4561 ccgggcggtg gagcactggc ccggggatgt cacgatcgag acggaggccc aggggtacgt
     4621 gctcgatcgg cccaggttcc tgacgcatgt catggacatg gcgaggaagg aagggtgcca
     4681 taccgaggtc ggggccgcgg tcgaggtcgg cagggacgag aacggataca tggtccgaac
     4741 ccgggacggg aggaggttcg gcgcaagg
//
LOCUS       DAOSYN010000096         4532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_15688_length_4532_cov_4.538977, whole genome shotgun sequence.
ACCESSION   DAOSYN010000096 DAOSYN010000000
VERSION     DAOSYN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15688_length_4532_cov_4.538977"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLJ11_04915"
     CDS             complement(<1..412)
                     /locus_tag="PLJ11_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04046.1"
                     /translation="MWKSLAKHFGKYPSQAKVAKLLLVHGLRVREGRVYCGDIEIADM
                     ALGRAAGVDRRIVRSAIDTIERSPELRAVYSRLVPTALLTEVAPVMGWTALEIIPTDA
                     ATPGIVAEVTGVIAREGISVRQTVVSDPDLSPDPR"
     gene            609..1886
                     /locus_tag="PLJ11_04920"
     CDS             609..1886
                     /locus_tag="PLJ11_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO04047.1"
                     /translation="MLLEFEVENFRSFADPVTFSMIASSDRSHADHLIAMGGSRDRAM
                     RSASIYGANASGKSNLVMAMGMLQSIVLRSHLAQLGEPMPYFPFKFGRPGEWTSTRYR
                     VVFLMNGVEYEYSLRHDAARVYEERLYHYPKNRKAIIFERVDGRELRAPQDKGSLRAT
                     AEKTLPNALLLSKAVQDNNPLVMPAFKWFQERLMVILKVNSMVVEERALARMQQDERF
                     RALLLKALNMADLGIVDVRGKVVQMDQEELDRMPPEIQEQMRGLVKGKIEQWRRLEIK
                     TVHQAVDEGGKVVQIEMDFHHEESLGTQKVFAVMALILEAMEAGRVLVMDEMDLHLHP
                     QIARFLVELMNDPEQNRGNAQFVFTTHNTNLIDLNLFRRDQIWFIEKDERGRSSLYSL
                     SDFNVRTDLRVQKAYEVGRFGAVPFVKGGKVIE"
     gene            1883..2509
                     /locus_tag="PLJ11_04925"
     CDS             1883..2509
                     /locus_tag="PLJ11_04925"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HOO04048.1"
                     /translation="MTRHRGRRSHPREPRPRLLIVCEGRRTEPNYFEGFRRENRNIII
                     MICESPGKTPMQIVRYAKERADEMQIGKVTGDSAWCVFDVDECTDKDMRRAIENAGRM
                     LRLAVSNPCFEFWFLLHYTYFDRRMDTCREVITELRRYIPSYEKSRDHFDLLRPMTST
                     AVDNAEGLERGRRGMGLEPYSRGANPCTGVHHLVTEILTRRRDPPRDG"
     gene            2656..3447
                     /locus_tag="PLJ11_04930"
     CDS             2656..3447
                     /locus_tag="PLJ11_04930"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOO04049.1"
                     /translation="MLGKSVRMERIMDRGTGRAVIVPMDHGMTMGPIDGLVDMRSTID
                     KVAQGGATAVVLHKGIIPYSHRSFGRDIGLIVHLSASTKFSKRTDQKILVTTVEEALK
                     IGADAVSIHVNIGNEYENEMLEDFGDISRRCNEWGMPLLVMIYPRGKDMKDAYDPDAL
                     KVCARVGTELGADIIKTSYTGDIDTFREVVRGAQAPVVIAGGPKMDSDERLLQMVRDS
                     IDAGGKGVSIGRNIFQHKNVIGITKAISGIVLEDMEVEEAMQLLK"
     gene            3452..4489
                     /locus_tag="PLJ11_04935"
     CDS             3452..4489
                     /locus_tag="PLJ11_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HOO04050.1"
                     /translation="MSGTGKIVWVRADHLPSAAERKRMVTSALEAGYADIVIREEDAA
                     LQRVGRYCAIVRKGDGLYVEGKRLGAVLTVREAKDVEGASRFKGKEEAVLVESHDWKV
                     IPLENLIAEFQGTGTKVIASCASVEEAKLFAATLESGVDGLAITIGDPAGLGRFSELR
                     RPEVATIELVEATVKGVRPVSVGDRVCVDTCSLLRVGEGMLVGSQSACLFLVQSESMD
                     SHYVASRPFRVNAGAVHAYVLGADGRTKYLSEVKGGTELLAVDSEGRTRPVVVGRAKV
                     EVRPLLLLEAEVGGRTYTTVLQNAETIRLCTRDGSISVADLKEGDKVLVRLEEGGRHF
                     GHAVKESVVER"
BASE COUNT          965 a         1254 c         1516 g          797 t
ORIGIN      
        1 ggcgggggtc cggggagagg tcggggtcgc tcaccaccgt ctggcgcacc gagatcccct
       61 cccgggcgat gaccccggtg acctccgcga cgatcccggg ggtggccgcg tccgtaggga
      121 tgatctccag cgccgtccag cccatgaccg gggcgacctc ggtgagcagg gcggtgggca
      181 ccaggcgcga gtagaccgcc cggagctccg ggctgcgctc gatcgtgtcg atggccgagc
      241 gcacgatgcg gcggtccacg cccgccgccc gccccagggc catgtccgcg atctcgatgt
      301 ccccgcagta cacccggccc tcccggaccc tgagcccgtg gaccaggagc agcttggcca
      361 ccttggcctg cgatgggtat ttgccgaaat gcttggcgag cgatttccac atcgatagta
      421 gtgatgtgtt ccaaccttat aatcgttgac agaagcgcgt aataatgcgc agatgtgcac
      481 gctcccatcc attaaagtgt gtagtccctt cggcgataat cgggatattt gtggtattaa
      541 tgattaaata atggtaatac aattggaaat ttgtgaataa ttcacaaaaa tacagtggag
      601 gattgtgaat gctcttagaa tttgaggttg agaactttcg ttccttcgcc gaccctgtaa
      661 cgttcagcat gatcgcttcc tccgacagga gccatgcgga ccatctgatc gcgatggggg
      721 gttccaggga ccgggcgatg cgatcggcat cgatctatgg ggcgaacgcc tcgggcaagt
      781 ccaacctcgt tatggccatg ggcatgcttc agagtattgt attgcgttcc caccttgcgc
      841 aacttggcga gcctatgcct tacttcccgt tcaagttcgg aaggccgggg gagtggacgt
      901 ccacccgata tagggtggtc tttctaatga acggcgtgga atacgaatat tcgctgaggc
      961 atgacgccgc ccgggtatat gaggaacgtc tctatcatta tcctaagaac cgtaaggcca
     1021 tcatcttcga gagggtcgat gggcgggaac tgagggcccc ccaggacaaa gggtcgcttc
     1081 gagctacagc tgagaagacc cttcccaacg cgctcctgct gtcaaaggcg gtacaggaca
     1141 acaatcccct tgtgatgcct gccttcaaat ggtttcaaga aaggctcatg gtcatcctga
     1201 aggtcaattc catggtcgtt gaggaaaggg ccctcgccag gatgcagcag gatgaaaggt
     1261 tcagagcgct cctcctcaag gccttgaaca tggcagacct agggatcgtc gacgtccgcg
     1321 gcaaggtcgt tcaaatggac caagaggagc tggacaggat gcccccggag atccaggagc
     1381 agatgcgagg attggtgaaa ggcaagatag agcaatggcg caggctggag ataaagactg
     1441 tccatcaggc cgtggatgag ggcgggaagg tagtacagat agagatggac ttccaccatg
     1501 aggaatcgtt ggggacccag aaggtcttcg ccgtcatggc gctgatcctg gaggcgatgg
     1561 aggccggccg cgtcctggtc atggacgaga tggacctgca tctgcaccct cagatcgcca
     1621 ggttcctggt cgaacttatg aacgatcctg agcagaaccg ggggaacgct cagttcgttt
     1681 tcaccacaca caacaccaac ctgatcgatc tgaacctttt caggagggac cagatctggt
     1741 tcatagagaa ggatgaaaga ggccggtcta gcctgtactc gctgtccgat ttcaatgtgc
     1801 ggacggacct tagggtccag aaggcgtatg aggtgggcag gttcggcgcg gtccccttcg
     1861 taaagggagg gaaggtcatc gaatgacgcg ccatagaggc cgtaggtccc atccaaggga
     1921 accaaggccc cgattgctga tagtctgcga aggaaggagg acggagccca actacttcga
     1981 gggtttccgg agagagaatc gcaatatcat catcatgatc tgcgaatccc ctgggaaaac
     2041 gccgatgcag atcgtccgct acgcgaagga aagggcggac gaaatgcaga taggcaaggt
     2101 cacgggggat tccgcctggt gcgtcttcga tgtagatgaa tgtacggaca aggacatgcg
     2161 aagggcgata gagaacgcag ggagaatgct gaggctggcg gtgtctaacc cttgcttcga
     2221 gttctggttc ctgctgcact acacttattt cgatagacgc atggacacct gccgggaggt
     2281 catcaccgaa cttcgaagat acatccccag ttatgaaaaa tcaagggacc atttcgacct
     2341 cctcagaccc atgacctcga cagctgtcga taatgcggaa gggctagaga gaggccgccg
     2401 agggatgggg cttgaaccct attcgagggg ggcaaatccc tgcactggtg tgcatcacct
     2461 cgtaacggag atcttgaccc gacggagaga ccccccgagg gatggctgat cgatgcgggg
     2521 gaaagatggc ccccattctg ccagaaccta gtaggccgtg gactggaaca tattaataaa
     2581 gcctacacat tatatttgtc aggaacgcaa cccttatatg cgtgctatta attgacacgg
     2641 caggaaggtg aaaaagtgct aggcaagagc gtaaggatgg aacgcatcat ggaccggggc
     2701 accgggaggg cggtcatcgt ccccatggac cacgggatga ccatgggccc catcgacgga
     2761 ctagttgaca tgaggtcgac catagacaag gtggcgcaag ggggcgccac cgcggtggtg
     2821 ctgcacaagg gcatcatccc gtacagccac cggtccttcg ggcgcgacat cggcctgatc
     2881 gtgcatctct cggccagcac caagttcagc aagcgcaccg accagaagat cctggtcacc
     2941 accgtggagg aggcgctgaa gatcggcgcg gacgcggtgt ccatccatgt caacatcggg
     3001 aacgagtacg agaacgagat gctggaggac ttcggggaca tctcccgccg ctgcaacgaa
     3061 tggggcatgc cgctgctggt catgatctac ccgcggggga aggacatgaa ggacgcgtac
     3121 gaccccgacg ccctcaaggt ctgcgcccgg gtggggacgg agctgggcgc ggacataatc
     3181 aagacgagct acaccggcga catagacacc ttccgcgagg tcgtccgggg agcgcaggcg
     3241 ccggtggtca tcgccggggg cccgaagatg gactccgacg agcggctgct gcagatggtg
     3301 cgcgactcca tcgacgccgg gggaaagggg gtctcgatcg gccgcaacat cttccagcac
     3361 aagaacgtga tcggcataac caaggccatc agcgggatcg tgctggagga catggaggtc
     3421 gaagaggcca tgcaactgct gaagtgaggg catgtccggg acgggcaaga tagtctgggt
     3481 gagggccgac cacctcccgt cggccgcgga gcgcaagcgc atggtcacct ccgccctgga
     3541 ggcggggtac gcggacatcg tcataaggga ggaggacgcc gcgctgcagc gggtcgggcg
     3601 ctactgcgcg atagtgcgca agggcgacgg gctctacgtc gagggcaagc ggctcggcgc
     3661 cgtgctcacg gtccgggagg ccaaggacgt ggagggggcc tcccgcttca aggggaagga
     3721 ggaggccgtg ctcgtcgaat cgcatgactg gaaggtcata ccgctcgaga acctcatcgc
     3781 cgagttccag gggaccggca ccaaggtcat cgcctcctgc gcgtcggtgg aggaggcgaa
     3841 gctcttcgcg gccaccctgg agagcggggt ggacgggctg gccatcacca tcggcgaccc
     3901 ggcggggctg gggcgcttct ccgagctgcg gcggcccgag gtcgccacca tcgagctggt
     3961 ggaggccacc gtcaaagggg tcaggccggt cagcgtcggc gaccgggtgt gcgtggacac
     4021 ctgctccctc ctccgcgtcg gcgaagggat gctggtcggc tcgcagtccg cctgcctctt
     4081 cctggtgcag agcgagtcca tggactcgca ttacgtcgct tcccgcccgt tcagggtgaa
     4141 cgccggggcg gtgcacgcct acgtcctggg cgcggacggc cgcacgaagt acctctcgga
     4201 ggtcaagggc ggcaccgagc tcctggccgt ggactcggag gggcgcacga ggccggtggt
     4261 cgtcggccgg gccaaggtgg aggtccgccc gctcctgctg ctggaggcgg aggtcggcgg
     4321 caggacgtac acgacggtcc tgcagaatgc ggagaccatc cgcctctgta ccagggacgg
     4381 ctccatctcg gtggcggacc tcaaggaggg ggacaaggtc ctggtgcgcc tggaggaggg
     4441 gggccggcat ttcgggcatg cggtgaagga gagcgttgtg gagcgatgac caggacctgc
     4501 gtggcgatct cggaggcgga cgcggcctcg gc
//
LOCUS       DAOSYN010000097         4505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_15812_length_4505_cov_2.977528, whole genome shotgun sequence.
ACCESSION   DAOSYN010000097 DAOSYN010000000
VERSION     DAOSYN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15812_length_4505_cov_2.977528"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..349)
                     /locus_tag="PLJ11_04940"
     CDS             complement(26..349)
                     /locus_tag="PLJ11_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO04051.1"
                     /translation="MKVAVTAGGPGTEAMMDDRLGRCPYFVILDTDTGKIDSLRNDAA
                     DASSGAGTKAMQALVDHEVSVVITGRIGPHATAMLEETGMKAVTNKSGKVGQVLEDFR
                     RERGL"
     gene            complement(346..615)
                     /locus_tag="PLJ11_04945"
     CDS             complement(346..615)
                     /locus_tag="PLJ11_04945"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOO04052.1"
                     /translation="MPYLDGTGPMGTGPYGRGMGPCRANGRISVRTGRGRRGGFGCQS
                     YCWSKPWRQLTDAEIKRSMEAEIEVLEARLKYLKDQLKVEGTDSS"
     gene            complement(692..970)
                     /locus_tag="PLJ11_04950"
     CDS             complement(692..970)
                     /locus_tag="PLJ11_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOO04053.1"
                     /translation="MKPRTLCGRSDYGSFMPSRPTDDEPIVLEMDEMEALRLVDYLGM
                     SQEEAAIRMGVSRGTVWRCVDSGRRKLVAMVEEGRPLKVRDDAPRIDD"
     gene            1078..1854
                     /gene="larE"
                     /locus_tag="PLJ11_04955"
     CDS             1078..1854
                     /gene="larE"
                     /locus_tag="PLJ11_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOO04054.1"
                     /translation="MDDAMVKYRKLIDLIRGMENVAIGFSGGVDSTLLLKAALDSGAE
                     VAAFMAVGETFPPEEQKAAISLAKGLGVEVVPLKVDLVNEGRFARNWEDRCYICKRLI
                     FEAIKEEAKELDIPHILEGSQVDDLGQARPGRAALMEMGIRSPLVEAGLTKAEVRSLL
                     KEMGVPNWDAPSNTCLATRVPHDVRITADILRRVNKAEAALAPFHFKELRVRDHEYWA
                     RVEVAKDDLPRLFQEREAVTKALKGLGYRRVAIDLEGHGD"
     gene            1894..2448
                     /locus_tag="PLJ11_04960"
     CDS             1894..2448
                     /locus_tag="PLJ11_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOO04055.1"
                     /translation="MRISITILQYDHGIIRQVLDVVGELLRSHRASRYGPELKEALTF
                     LEQFLDRFHHAKEERFLFPAAMEECPKVAETLALLKKEHVQARRTVRSALRALEEGNV
                     EKAERDAMRLVERITLHISEEENRVFPVIENFLAMETDAKLYEQYQAFMQKKFGAGYY
                     QVAEAFANDIQDRVLGPGFFKGIV"
     gene            complement(2453..2559)
                     /locus_tag="PLJ11_04965"
     tRNA            complement(join(2453..2500,2522..2559))
                     /locus_tag="PLJ11_04965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2523..2525),aa:Ser,seq:cga)
     gene            2671..2880
                     /locus_tag="PLJ11_04970"
     CDS             2671..2880
                     /locus_tag="PLJ11_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04056.1"
                     /translation="MSELVLTVEMERKEREMRLPEGSTAGSVLDLLGLHPDAHIVVRG
                     RTPVPIDSPLVPGDRLRIVKVASGG"
     gene            3150..>4505
                     /gene="dnaG"
                     /locus_tag="PLJ11_04975"
     CDS             3150..>4505
                     /gene="dnaG"
                     /locus_tag="PLJ11_04975"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HOO04057.1"
                     /translation="MNIDPSTTKYLIKVKLNADGIVEKPDVVGAIFGQTEGLLGDELD
                     LRDLQKSGRIGRIEVDVHSRQGKSEGQILIPSSLDQVETVILASALETIDRVGPCKAK
                     IGVESIEDVRIVKRERIIERARELLTELIKQSKSSGIDLTESVRQSVQVEEITYYGKD
                     RLPAGPNVAESDAIIVVEGRSDVLNLLKSGIKNAIAVEGTNIPKTISDLSKERVITAF
                     VDGDRGGELILRELFQVAEVDFVARAPRAHEVEELTQKQIMKCLRNKIPGDQFIEMFN
                     LELGEGNGKDKERRPEPSAGKVEKLERFEKAERADREREEKEDTAKAEHEPKQEREPR
                     QERAERREREPRMERREQEEPKPEREPRSERQEREPRPERPPVEEREHEERRPVSRKQ
                     LSAAQEKYKGMINELASTSKARILDAMGGITKEVTVKELVNTLKEEKGPVSAVVFDGI
                     IT"
BASE COUNT          940 a         1303 c         1464 g          798 t
ORIGIN      
        1 acctggaata gaaaggatga gggggtcaca gacccctttc gcgcctgaag tcctcgagga
       61 cctgccccac cttgcccgac ttgtttgtga ccgccttcat gccggtctcc tccagcatcg
      121 cggttgcatg cgggcctatc cggccggtga tgaccacgct gacctcatgg tccaccaggg
      181 cctgcatggc cttggtcccg gcgccgctgg aggcgtctgc ggcatcgttc cttagtgaat
      241 cgatcttacc ggtatcggtg tccaatatca cgaagtacgg gcatcggccc agcctatcgt
      301 ccatcatagc ctcggtcccg gggccgccgg cggtgaccgc caccttcatg aggaatcagt
      361 cccctcgacc ttcagctggt ccttgagata cttcaacctg gcctccagga cctcgatctc
      421 tgcctccatg gagcgtttga tctccgcatc ggtcagctgc ctccagggct ttgaccagca
      481 ataagattgg cagccgaagc cacccctcct tccgcggccc gttctaacgc ttatcctgcc
      541 gttcgcgcgg caaggtccca ttccccggcc gtagggtcct gtgcccatgg gtccggtacc
      601 gtctagatat ggcatgtttg catcaccttt cgccgtgtta acggcgtcta taatgtgcat
      661 aagcacaata ttataaataa tttagggaag cctaatcatc aatgcgtggt gcatcatcgc
      721 gcaccttgag cgggcgccct tcctccacca tggcaacgag cttccgcctc ccactgtcca
      781 cacatctcca gacagtgccc cgcgagaccc ccattctaat ggccgcctcc tcctggctca
      841 tccctaggta atcgactagg cgcagcgcct ccatctcgtc catttccaaa actatcggtt
      901 catcatcggt tggacgggat ggcatgaagc tgccatagtc cgatcggccg cataatgtgc
      961 gcggcttcat gggccttccc gggcttttcc ttccgaagcg gcactgattt ccaggcatat
     1021 gaaggcaatg gtctggcctc ataataaata tccccgctcc catcatcggt cgatagcatg
     1081 gacgatgcga tggtcaagta ccgcaagctt atagacctca tacgaggcat ggagaacgtg
     1141 gcgataggtt tctccggagg ggtggatagc accctactcc tgaaggccgc attggactcc
     1201 ggcgcggagg tcgcggcgtt catggctgtg ggggagacct tccctccgga ggagcagaag
     1261 gccgccatct ccctggcgaa ggggctgggc gtggaggtcg tccccctgaa ggtggacctg
     1321 gtcaacgaag gacggttcgc ccgcaactgg gaggaccgtt gctatatatg caagaggctg
     1381 atcttcgagg ctatcaagga ggaggcgaag gagctggaca tcccgcacat attggaagga
     1441 agccaggtcg acgacctcgg gcaggcccgt cccggccggg cggccctgat ggagatgggg
     1501 atccgctccc ccttggtcga ggcggggctg acgaaggcgg aggtgcgctc cctgctcaag
     1561 gagatggggg tcccgaactg ggatgccccc agcaatacct gcctggccac cagggtcccc
     1621 cacgacgtgc gcattaccgc ggacatcctc cgcagggtga acaaggccga ggccgccctt
     1681 gccccgttcc acttcaagga gctgcgggtg cgggaccatg agtattgggc gagggtggag
     1741 gtggcgaagg acgaccttcc ccgcctcttc caggagcggg aggcggtgac gaaggcgctg
     1801 aaagggcttg gctaccggcg ggtggcaatt gacctcgagg gccatggcga ttgagccaaa
     1861 ccaataaaaa ggtggcgtgc cctagtagcg ggcatgagga tctcgataac catcctgcag
     1921 tatgaccatg gcatcattcg gcaggtgctc gatgtggtcg gcgaactcct gcgctcccat
     1981 cgcgcctccc gatatggccc ggagctcaaa gaggccctga cctttctgga gcagttcctg
     2041 gaccgcttcc atcatgccaa ggaggaacgg ttccttttcc cggccgcgat ggaggaatgc
     2101 cccaaggtgg cggagaccct agcgctgctg aagaaggaac acgtgcaggc ccggcgcacg
     2161 gtgcgaagcg ccctcagggc gctggaggaa gggaacgtgg agaaggcgga gagggacgcc
     2221 atgcgcctcg tggagcgcat caccctgcac atcagcgagg aggagaaccg ggtctttccg
     2281 gtgatcgaga acttcctggc gatggagacg gacgccaagc tctacgagca gtaccaggcg
     2341 ttcatgcaaa agaagttcgg ggcggggtac tatcaggtgg ccgaggcctt cgcgaacgac
     2401 attcaggaca gggtgctggg gccggggttc ttcaagggca ttgtttgatg gatggcgcca
     2461 aggaggagat ttgaactccc gagacatgaa gtcagtagct gtcccatcgg caaagggccg
     2521 attcgaggct accgccttac cggactaggc taccttggcc cggatgggca tttgcctgct
     2581 cctatttcaa acttgaccgg tatcattgga acagaggttg atcggaagaa caggaaaagg
     2641 attaatccct aggagggatt gggactgccg atgagcgagc tcgtgcttac ggtggagatg
     2701 gagcgcaagg agcgggagat gcgccttccc gaagggtcga ccgcggggag cgtgctcgat
     2761 ctcctcggcc tgcatcccga tgcccacatt gtcgtccggg ggaggacccc cgtgccaatc
     2821 gattcgcccc tcgtccccgg cgaccgcctc cgaatagtga aggtggcctc gggcgggtga
     2881 gcggcttggc ccgagcttcc ggagaggggg ccgtgcataa agtatataaa ggtcccctat
     2941 ttttacggta ttgtcgatca aagcctggag aaggacccgg gctttcctat atcacatgat
     3001 cactatcacc ctttcatcgc gcggcctggt gaggccgggt accggatgga aaaccccttc
     3061 gggatcgtca ggactagggt cttgatgggc atagtgggcc ttggtgtgcg aaagcctcgt
     3121 tctttcttca gctggacaga ggtgacaaca tgaacattga cccgagcacc accaagtact
     3181 tgataaaggt caagcttaac gctgacggta tagtcgagaa gccggacgtg gtgggagcta
     3241 tcttcggaca gacggaaggg ctgttgggcg acgagctcga cctgagggac ctgcagaaga
     3301 gcggcaggat aggcaggatc gaggtggacg tccactcccg ccagggcaag tccgaaggcc
     3361 agatattgat accatctagc ttagaccaag tagagaccgt gatcttggcc tccgcgctgg
     3421 agaccatcga cagggtgggc ccgtgcaagg ccaagatcgg cgtggagtcc atcgaggacg
     3481 tgcgcatcgt caaacgcgag aggatcatag agagggccag ggagcttctg accgagctca
     3541 tcaagcagtc caagtcctcc gggatcgacc tcaccgagag cgtccgccag tccgtgcagg
     3601 tggaggagat cacctactac ggcaaggacc gcctccccgc ggggccgaac gtcgccgagt
     3661 ccgacgccat catcgtggtg gagggacgct ccgacgtgct gaacctgctg aagtcgggga
     3721 tcaagaacgc catcgcggtc gaggggacga acatcccgaa gaccatctcg gacctcagca
     3781 aggagagggt gatcaccgcc ttcgtcgacg gggacagggg cggggaactc atcctgcgcg
     3841 agctgttcca ggtggccgag gtggactttg tcgcccgggc gccgcgcgcc cacgaggtgg
     3901 aggagctcac ccaaaagcag atcatgaagt gcctgcgcaa caagatcccc ggcgaccagt
     3961 tcatcgagat gttcaacctg gagctgggcg agggcaacgg caaggacaag gagcgccggc
     4021 cggagccgag cgctggtaag gtcgagaagc tggagcgctt cgagaaggcc gagagggcgg
     4081 accgcgagcg ggaggagaag gaggacaccg ccaaggccga gcatgagccg aagcaggaga
     4141 gggagcctcg tcaggaaagg gcggagcgcc gggagcgcga gcctcggatg gaacgccggg
     4201 agcaagagga gcccaagcct gagagggagc ccaggtcgga gaggcaggag cgggagccta
     4261 ggccggagag gcctccggtc gaggagcggg agcatgagga gcgcaggccc gtctcccgga
     4321 agcagctctc cgccgcccag gaaaagtaca aggggatgat caacgagctc gcgtccacct
     4381 ccaaggcccg catcctcgat gccatgggcg ggataacgaa ggaggtgacg gtcaaggagc
     4441 tggtcaacac attgaaggag gagaaggggc ccgtgtccgc ggtcgtcttc gacggcatca
     4501 tcacc
//
LOCUS       DAOSYN010000098         4499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_15836_length_4499_cov_3.249325, whole genome shotgun sequence.
ACCESSION   DAOSYN010000098 DAOSYN010000000
VERSION     DAOSYN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15836_length_4499_cov_3.249325"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2623
                     /locus_tag="PLJ11_04980"
     CDS             <1..2623
                     /locus_tag="PLJ11_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOO04058.1"
                     /translation="REAVAGRLREWYRAAPLFRRLVVIGPFEPTPSQASLLRALVEVC
                     EEVIYHHPFVPGHGKVFVQGTVDLGREMEVLDLPPDERDMRRLALIRPWGEGRVDLGA
                     ECAEGRFLDPLAETRQGAQRIATLLEQGVDPNSIAIYLPERREALPLVRDVLSDFEIP
                     FRTDLGAPLSTSPAVQAALSVLDAVDQAYSPGSIRRLLSSPYLRWPREGERLWHVDVD
                     RLARMAGVRSGRRAWSAGLERLMAELQAQAEDPEVPEARRAIALRDRERAAAPPPALE
                     ALLDELGGLEGNKGIAGHVAAFRDALERLGAVEAVGRHAREEGNEARAFQGLLRTLAA
                     LEAEGRVDEGAIPPQGFLAELRRQLAGATYQPEGTNDLGVMVGGYRSLEGHEFEHSFL
                     AFTVEGDMPKLGVRHPFITSLQARAIGLLGEEDILRQERYYFLGAFMGGASVLISHPF
                     YQEGKRALPSPFLQDLERKCTVAPMEEVPITRSRRCAQMALGTMLAGGSVPAPELWSD
                     RETLPPAELCARLNVERGRQGEYRSPHDGVLSDPEVLETLAGDLGAKVFSPSMLEAYR
                     KCPMHYLLRYVLYLAPLEEDLEAMRLGTAAHEVLCRFYRRRAERGLGMPSPDEGLTAV
                     KREIREIGREVAARFKGEGADSEARYRALIGDEGYDGTLGTFVDRQATADLPRWRPEY
                     LEYSFGSRVAGDRYDPRSTERPAVIPLTEGAGDVLLLRGKVDRVDVDGNAMLVIDYKT
                     GKAPSYKSLEKGYSMQLQLYLLACAQLLGLEPAGGAYYQLKDDGFGMQLRVADPRYKD
                     ALGGTPTPYATGLRKDLERALSNAREALEGMAAGAFHPVDSLEQERCPHSCPYSRICR
                     KDEMRVLAMTLARGEGR"
     gene            2620..>4499
                     /locus_tag="PLJ11_04985"
     CDS             2620..>4499
                     /locus_tag="PLJ11_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO04059.1"
                     /translation="MTPDRSVCVNASAGSGKTTRLVEQYFEHIEGGAPIGAVLALAFN
                     DEAAANMRRRIRERTMARGDERTFDQLHWAQVMTFHSFCAQVLKEFHLQAGVPSNFTV
                     IEDMDLAVLMDQAWEELMVTPDPALRREIVDACTAIGDRRLRQTITFLYERRARAAEA
                     LAAMEDREAFLEGYRRTCQGRLREVLGRFDDPAFTGAVGTLLRLADRYAKEDGGRAAS
                     YLHKARPLLLACGDGDQRTRLAALRGLLEVKGAKNMGDKGMGKDKDALGAAYATLRTI
                     LEAEEEELLYLPFDLDPDHQEAAVGHLMALRAIFLAYAALMDRRKAKGNALDFNDLLL
                     RAQWLLSPDRPDHQVLDVLRERYRYVLVDEYQDTDLPQDRIVRALLGGEKGKLFVVGD
                     GKQSIYLFRGADVSIFKDMRRFVRTELQGAEEEQENNFRSCPEVVAFVNRLFGRIMDR
                     EEADWEFKYAEVRAYRTLDRGSVTVVQVAKGDADAKLAMARAVADQIERLVAGDGRAV
                     HPGKEEMPRPPRYGDVAVLLRGRTNLRYYEGVLAERGIPYAVEKGIGLFQRQEVMDIA
                     NALAFLGNQADDVALYGILRSPYFGLSDETLCRVVAASSGQLFSLLRRFAEARPDEVG
                     AG"
BASE COUNT          744 a         1427 c         1700 g          628 t
ORIGIN      
        1 gagggaggcc gttgccggaa ggctgcgaga atggtaccgc gcggcaccgc tcttcaggag
       61 gctggtggtc ataggcccct tcgagcccac cccctcccag gcctccctgc tccgggcgtt
      121 ggtggaagta tgcgaggagg tcatctacca tcacccgttc gtccccgggc acgggaaggt
      181 gttcgtgcag gggaccgtgg acctgggaag ggagatggag gtcctcgacc tcccgcccga
      241 cgaaagggac atgaggaggc tggcgctcat caggccctgg ggggagggca gggtggacct
      301 gggcgccgag tgcgccgagg ggcgcttcct cgatcccctg gccgagacca ggcagggggc
      361 gcagcggatc gccacgctgc tggagcaggg ggtggacccc aactcgatcg ccatctacct
      421 cccggagagg agggaggccc tcccgctcgt gcgcgacgtg ctctccgact tcgaaatccc
      481 cttccggacc gacctgggcg ccccattgtc cacctcgccc gcggtgcagg ccgcgctctc
      541 ggtcctggac gcggtcgacc aggcctattc ccccggctcg atcagacgcc tgctctccag
      601 cccctatctc cgatggccga gggagggcga gcgcctatgg cacgtggatg tggaccgcct
      661 cgcccggatg gcgggggtcc gttcgggaag gagggcatgg agcgcagggc tggagcggct
      721 catggccgag ctgcaggcgc aggcggagga ccccgaggtc cccgaggcga ggagggcgat
      781 cgccctgagg gacagggaga gggcggcagc accgccgccg gccctcgagg cgctgctcga
      841 cgaactcggg ggattggagg ggaacaaggg catcgcgggg cacgtggcgg cgttccggga
      901 cgcgctggag cgcctcgggg ccgtcgaggc cgtcggcagg catgcccgcg aagaggggaa
      961 cgaggccagg gccttccagg ggctgctccg gacgctggcg gcgctcgagg cggagggcag
     1021 ggtggacgag ggggccatcc ccccgcaggg cttcctggcc gagctgagaa ggcagctcgc
     1081 cggggcaacg taccagcccg agggaacgaa cgacctgggg gtgatggtcg gcggctacag
     1141 gtccctggag ggacacgaat tcgagcacag cttcctggcg ttcacggtcg agggggacat
     1201 gcccaagctc ggcgtgaggc atccgttcat cacctccctg caggcccggg ccatcgggct
     1261 gctgggcgag gaggacatcc tgcggcagga acggtactac ttcctgggcg ccttcatggg
     1321 cggcgcctcc gtccttatct cccacccctt ctaccaggag gggaagaggg cgctgccctc
     1381 ccccttcctg caggacctcg agaggaaatg caccgtcgcc ccgatggagg aggtgccgat
     1441 cacccgctcc cggcgctgcg cccagatggc gctcggaacg atgctggcgg gcggcagcgt
     1501 ccccgccccg gagctctggt cggacaggga aacgctcccc ccggcggagc tgtgcgcacg
     1561 gctgaacgtg gagcggggca ggcaaggcga gtaccgctcg ccgcacgacg gcgtcctctc
     1621 cgacccggag gtgctcgaga cgctcgccgg ggacctgggg gcgaaggtgt tctcgccgag
     1681 catgctggag gcctatcgca aatgcccgat gcactatctc ctcagatacg ttctctacct
     1741 cgcccccctg gaggaggacc tggaggccat gcgcctggga accgccgccc acgaggtcct
     1801 ctgccggttc tacaggcgaa gggcggagcg gggactcggg atgccctccc ccgacgaggg
     1861 gctgacggcg gtgaagcggg agatcaggga gattggcagg gaggtggccg cccggttcaa
     1921 gggcgaaggg gcggacagcg aggcaaggta ccgcgcgctc atcggggacg aggggtacga
     1981 cggcaccctc ggcacgttcg tggaccggca ggcgacggcc gacctgccga ggtggaggcc
     2041 cgaatacctc gagtacagct tcggctccag ggtggcgggg gacaggtacg atccccgttc
     2101 caccgagcgg ccggccgtca tcccgctgac ggagggggct ggggacgtcc tgctgctgag
     2161 gggcaaggtg gaccgggtgg atgtggacgg gaacgccatg ctcgtcatcg actacaagac
     2221 gggcaaggcc ccctcctaca agagcctgga gaaagggtac agcatgcagc tgcagctgta
     2281 cctgctcgcc tgcgcccagc tcctgggcct ggagcccgcc gggggcgcgt actatcagct
     2341 gaaggacgac gggttcggca tgcagctgag ggtcgccgac ccccggtaca aggatgcgct
     2401 cggagggacc cccaccccct atgccacagg gctgcggaag gacctcgagc gcgccctgag
     2461 caacgccagg gaggcgctgg aggggatggc ggcaggcgcg ttccacccgg tcgattccct
     2521 cgagcaggag cggtgccccc actcctgccc ctactcccgc atctgccgca aggacgagat
     2581 gcgggtgctg gcgatgaccc tggccagggg ggaggggcga tgacgcccga ccgctcggta
     2641 tgcgtgaacg cctcggccgg ctcggggaag accacccggc tcgtggagca gtacttcgag
     2701 cacatcgaag ggggagcgcc gataggcgcc gtgctcgccc tcgccttcaa cgacgaggcc
     2761 gcggcgaaca tgcgcaggcg gataagggag aggacgatgg cgcgggggga cgagcgaacg
     2821 ttcgaccagc tgcactgggc ccaggtgatg accttccact ccttctgcgc ccaggtgctt
     2881 aaggagttcc accttcaggc cggggtgcct tccaacttca cggtcatcga ggacatggac
     2941 ctcgccgtgc tgatggacca ggcctgggag gagctgatgg tcacccccga ccccgccctg
     3001 cgcagggaga tcgtcgacgc ctgcacggcg atcggggacc ggcggctgcg ccagaccatc
     3061 acgttcctgt acgagcggag ggccagggcg gcggaggccc tggcggcgat ggaggaccgc
     3121 gaggcgttcc tcgagggcta caggcgaacg tgccagggcc gtctgcgcga ggtcctcggg
     3181 aggttcgacg accccgcctt caccggggcg gtgggaacgc tcctgcggct tgcggacagg
     3241 tacgccaagg aggacggggg cagggcggcc tcctacctgc ataaggcgag gccgctgctc
     3301 ctggcctgcg gggacgggga ccagcggacg aggctggccg ccctgcgggg gctgctggag
     3361 gtgaaggggg cgaagaacat gggcgacaag gggatgggga aggacaagga cgccctcggc
     3421 gccgcctatg ccaccctgcg gaccatcctg gaggcggagg aagaggagct gctctacctc
     3481 ccgttcgacc tcgatcccga tcatcaggag gcggccgtcg ggcatctcat ggccctcagg
     3541 gccatcttcc tggcgtatgc ggccttgatg gaccgcagga aggcgaaagg gaatgccctt
     3601 gacttcaacg acctgctgct ccgggcgcag tggctgcttt cccccgaccg gccggaccat
     3661 caggtgctgg acgtcctcag ggaacggtac aggtacgtgc tggtggacga gtaccaggac
     3721 acagacctgc cgcaggaccg catcgtgcgc gcgctcctcg gaggggaaaa gggcaagctc
     3781 ttcgtcgtcg gggacggaaa gcagtccatc tacctcttcc gcggggcgga tgtgagcatc
     3841 ttcaaggaca tgcggcgctt cgtgcggacg gagctgcagg gggcagagga ggagcaggag
     3901 aacaacttcc gctcctgccc cgaggtggtg gccttcgtca accggctgtt cggccgcatc
     3961 atggacaggg aggaggcgga ctgggagttc aagtacgccg aggtgcgggc gtaccgtacg
     4021 ctagaccggg ggtcggtgac cgttgtccag gtggcgaagg gggacgcgga cgccaagctg
     4081 gccatggcca gggcggtggc cgaccagatc gagcggctgg tggcggggga cggcagggcc
     4141 gtgcaccccg gtaaggagga gatgccgagg cctcccaggt acggggacgt cgccgtgctc
     4201 ctccgcggga ggacgaacct ccgctactac gagggcgtgc tcgcggagcg gggcatcccc
     4261 tatgcggtgg agaagggcat agggctgttc cagaggcagg aggtgatgga catcgcgaac
     4321 gcgctcgcct tcctgggcaa ccaggcggac gacgtggccc tgtacggcat actgaggtcc
     4381 ccctacttcg gcctctcgga cgagacgctg tgccgtgtgg tcgccgcctc ctccggccag
     4441 ctgttctccc tcctgcggag gttcgccgag gcgaggccgg acgaggtggg ggccggaag
//
LOCUS       DAOSYN010000099         4453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_16021_length_4453_cov_3.509322, whole genome shotgun sequence.
ACCESSION   DAOSYN010000099 DAOSYN010000000
VERSION     DAOSYN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16021_length_4453_cov_3.509322"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLJ11_04990"
     CDS             <1..1029
                     /locus_tag="PLJ11_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOO04060.1"
                     /translation="GVPVFPSFDSTKYVQYLLFIFAYYYLAAVVLVLALSYIALRGRF
                     RRELWALRPYQTAFFGGIVLAGTAMAWRGWGGPELISRIFEPPYWVNLAYVVPTVLSA
                     VLAWQAIVLWNDISDREWDSPTKEGRVIASGLLPVGVFKEASGVLAFVSLAVSLLLSV
                     QQFLIMAGILVLAFVYSFPPVRFKNALLSPILMGAGAFLAFLYGAATPFSVVSSVVDA
                     PYLTGEVFHPSLYPEIVMVGLFMFIGLVVGSIITDVDGYEEDRVGGVRTVYTVLGLER
                     GVDCVAVLIFLTSLTPLVLFHSAPDLVAFPALGLAAALWFRRARRSRPVMSIAMVGLL
                     YAALRFLL"
     gene            complement(1031..2206)
                     /locus_tag="PLJ11_04995"
     CDS             complement(1031..2206)
                     /locus_tag="PLJ11_04995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO04061.1"
                     /translation="MPLYDVAISGGGPSGSYCAYRCAQAGLSTVLIERRVPPWTKVCA
                     GGVLLRAASRLDFPLPSAIIEKEVRGLLVAGEGFRERFRFDRGLAYTVHRPNLDTHLL
                     GMAERAGAELMLGDEVKVIHEGSDVELHLRSSTVRARCAVVAEGAGSGNAHRLLGPPP
                     KDGMAMGLRSICPTIEGPGDEIEVFFIDSPTRGIRRPPMFPLMGWMFPYRGMANLGVG
                     GHGYSKAQLMAGMDHVLRHTSVRVASAPGPPSAHPIPIIPRPKVATRRTMLIGDSAGL
                     VSPLSGEGLSHGLASSILAAEAAIKLASGDKGAILAYEDAVASTIIRDIKAAAIISPV
                     LHWLLGVVDTGAFFRTVGKEEEYIRAWTEMALGEETWQRLLMMTIPRFHRLFFRSLA"
     gene            complement(2214..>4453)
                     /locus_tag="PLJ11_05000"
     CDS             complement(2214..>4453)
                     /locus_tag="PLJ11_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOO04062.1"
                     /translation="EWSTLPYIICSAFDALPGQQADMTSHWSGWYGSSNTGTANEDVT
                     VWSENRGSFVSRYGEYFISTNEIVGAIKAGDFDGDGDLDLVASFLHTVVYSTATDPDD
                     ADYSNSYGMFFNRGIYVFWNDGSWTKTQVYGTNVYTNQNDNPAALDLAVADLNQDGVD
                     DIVAVYETGVTKIWLNQWRQVIGISSDPKADAFGTGSPVPAANVPTVTGTNPWDHTQR
                     FPTVAIADVDLNGYPDIIRTSTTASGTSRTVTVIRTMPSTPSDILRTPSSEHSPESEV
                     SAKTTGSIANLTANDGSFETLSEVYKNTPIMLGLPDQTQASSPNIDDTGQDLADLRYD
                     DGMMYNVEALKTMHIRSFALDSTYSSKPVTQAILRVKYSVTDNYNGNNYIQYSLDGST
                     FVDTTLRPTSSSWNVNQTFDLKAMGIDTWSELHSLRVMFKHNGINGTVQFDYVRLEVR
                     FAESRWMEWVYEVPNVATQVTHQLGMSAYTTGETFTVKYSTDGTIWYDAFAFNETVET
                     VKQVQLPHTTNSVYYIKVSAADASTTDVQNDSLVIDIIGIRHISPTVQWPSADLRTVS
                     MTMQNSAEYITCLAVGDVGSTLNKLPDGYPDIIVGTSFVGSGSSTYTLMLSYGNGNSF
                     SAPESLATSGLSAAVGGNNAIYDTKSIALGDFNGDGYLDIAIVIGFAPGRSGGTAPSI
                     WLYRNDPSVGQWGEQVLNALATGDSAINIQAGNVDLTVLYPLLGILGLVAVEGWMARA
                     GRKRR"
BASE COUNT          738 a         1332 c         1416 g          967 t
ORIGIN      
        1 ggcgttccag tgttcccgag ctttgatagt accaagtatg tccaatatct cctcttcatc
       61 ttcgcgtact attacctggc ggcggtggtc cttgtccttg ccctcagcta catcgccctg
      121 agaggacggt tccgtcgaga gctgtgggcc ctgcgtccct atcagaccgc gttcttcggc
      181 ggaatcgtgc tcgctgggac ggccatggcc tggcggggat ggggcggacc ggaattgatc
      241 agcaggatat tcgagccccc atactgggtt aacctggcct atgtcgtgcc gaccgtgctc
      301 tcggccgttc tcgcctggca ggccatcgtt ctttggaatg atatcagcga ccgcgaatgg
      361 gattctccga ccaaggaggg gagggtgatc gcctcgggcc tccttccggt cggggtgttc
      421 aaggaggctt cgggggtcct cgccttcgtg tccttggccg tatcgctgct gctgagcgtg
      481 cagcagttcc tcataatggc ggggatactt gtattggcct tcgtgtactc cttccctccg
      541 gtgaggttca agaacgccct gctgagcccc attctcatgg gcgcgggggc gttcctggcg
      601 ttcctctacg gggcggcgac acccttcagc gtggtcagct ccgttgtcga tgccccatac
      661 cttactgggg aggtgttcca tccttcgctt tatccggaga tcgtgatggt ggggctgttc
      721 atgttcatcg ggctggtcgt cggatcgatc ataacagatg tcgacggtta tgaggaggac
      781 cgtgtcggag gggtgaggac ggtctacacc gtcctgggcc tggaacgggg ggtggactgc
      841 gtggccgtcc tcatcttcct gacctccctg acccccctgg tgctgttcca ttctgcaccg
      901 gacctcgttg catttccagc cctgggattg gcggctgccc tgtggttccg tagggccagg
      961 cggtcgaggc cggtgatgtc catcgccatg gtggggctgc tctacgccgc cctgcggttc
     1021 cttctgtagg ctaggccaag gagcggaaga agagccggtg gaagcgcggg atggtcatca
     1081 tcaggaggcg ctgccatgtt tcctctccga gcgccatctc ggtccaggcc cttatgtatt
     1141 cctcctcctt tccgacggtc cggaagaagg cccccgtgtc aacgaccccc aggagccaat
     1201 gcaggaccgg ggagatgatc gcggcggcct tgatgtccct gatgatggtg gaggccaccg
     1261 catcctcata ggcgaggatg gcccccttat ctccggaggc cagtttgatc gctgcctccg
     1321 ccgccaggat ggagcttgcc aacccgtgcg acagcccttc cccgctgagc gggctgacca
     1381 gccccgcgct gtccccaatg agcatcgtcc tacgtgtggc caccttcggc cgtgggatga
     1441 tggggatggg gtgggcggag gggggccctg gcgcactggc taccctcacc gaggtatgcc
     1501 ttaggacgtg gtccatccct gccatgagct gcgccttgga atagccatgc cctcccacac
     1561 ccaggttggc cattccacgg taggggaaca tccagcccat cagggggaac atggggggac
     1621 ggcggatgcc ccttgtcggg ctgtcgatga agaagacctc tatctcgtcc ccaggcccct
     1681 caatggtcgg gcagatggag cgcaggccca tggccatacc gtccttcggc ggcggcccga
     1741 gcaaccggtg ggcgttcccg ctgcccgccc cctccgcgac cactgcgcat ctggccctga
     1801 cggtggagct tcggagatgg agctccacgt cgctcccttc gtggatcacc ttgacctcgt
     1861 ccccgagcat cagctccgcc cctgccctct cggccatacc gagcaggtgc gtgtcgaggt
     1921 tgggccggtg gaccgtgtag gccaggccac ggtcgaaccg gaagcgctcc cgaaagccct
     1981 cgcccgccac caggaggccg cggacctcct tctcgatgat ggctgaggga agcgggaaat
     2041 ccagcctgga ggccgcccgc agcagcaccc cgcccgcgca gaccttggtc cagggaggga
     2101 cgcgtctctc gatcagaacg gtcgagaggc ccgcctgggc gcagcggtag gcgcaatagg
     2161 accccgaggg tcccccgccg gagatggcca cgtcgtacag gggcacgcgg gggtcacctg
     2221 cgcttgcggc ccgccctggc catccagccc tccactgcca ccaggcccaa tatcccgagg
     2281 aggggatata ggacggtcag gtccacattg cccgcctgga tgttgatcgc ggaatctccg
     2341 gtcgccaatg cgttcagcac ctgttccccc cattgcccga ccgatgggtc gttgcggtac
     2401 agccagatcg atggcgccgt gcccccgctc ctcccaggcg cgaagcctat cactatggcg
     2461 atgtccagat agccgtcgcc gttgaagtcg ccaagcgcta tgctcttggt gtcatagatg
     2521 gcattgttcc cgccgacggc ggccgagagg ccggaggtcg ctaatgactc cggggcgctg
     2581 aaggagttgc cgttgccgta ggatagcatt agggtgtatg tggacgagcc gcttccgacg
     2641 aaggacgttc cgacgataat atcgggataa ccgtcgggca gtttgttgag tgtggacccg
     2701 acatccccta cggccagaca tgtaatgtac tcggcactgt tctgcatggt catgcttacg
     2761 gtcctaaggt ctgctgaggg ccattgcacg gtcggcgata tgtgccttat cccgatgatg
     2821 tcgatcacca gggaatcgtt ctgcacatcg gtcgtggagg cgtcggccgc ggagaccttg
     2881 atgtagtata cagaattggt ggtgtgcggc agctggacct gcttgaccgt ttcaacggtc
     2941 tcgttgaagg cgaacgcatc gtaccagatg gtgccgtccg tggagtattt cacggtgaac
     3001 gtctctcccg ttgtataggc ggacatgcca agctggtggg tgacctgggt ggcgacgttc
     3061 ggcacctcgt acacccactc catccaacgg ctctccgcga acctgacctc cagccggacg
     3121 tagtcgaact ggacggtacc gtttatcccg ttgtgcttga acatgaccct gagcgaatgg
     3181 agctcggacc aggtgtcgat gcccatggcc ttcaggtcga aggtctggtt cacgttccat
     3241 gaggaggagg tcggtctcag ggtcgtgtca acgaacgtgc tgccatccag ggagtactgg
     3301 atgtagttgt tgccgttgta gttgtccgtg acggagtatt tgaccctcaa tatggcctgg
     3361 gtcacgggct tgctggagta tgtgctgtcc agggcgaagc tccggatgtg catcgtcttc
     3421 agcgcttcga cgttgtacat catcccgtca tcatacctga ggtcggccag gtcctgccct
     3481 gtgtcatcta tgttgggcga tgaggcctgg gtctgatcgg gaaggcctag catgatgggc
     3541 gtgttcttgt atacctcgct cagggtttcg aaggacccgt cgttcgccgt aaggttggcg
     3601 atcgacccgg tggtcttggc gctgacctcg gactccgggg aatgctccga tgatggggtc
     3661 ctgagtatgt ccgagggcgt ggaaggcatc gtcctgataa cggtgacggt cctggatgtg
     3721 ccagaggctg tggtgcttgt ccggatgatg tccgggtatc catttaggtc gacgtccgct
     3781 atggcgaccg ttgggaacct ctgcgtgtgg tcccaggggt tggtcccagt gaccgttggc
     3841 acgttcgctg ccggcacggg cgagcccgtg ccgaaggcat cggccttggg atcggaggag
     3901 atcccgatga cctgcctcca ctggttgagc cagatctttg tgacgccggt ctcgtacacg
     3961 gctacgatgt cgtccacccc gtcctggttg aggtcggcga ccgctaggtc cagggcggcg
     4021 gggttgtcat tttgattggt atatacattg gtaccgtata cctgggtctt ggtccagctc
     4081 ccatcgttcc agaacacgta gatgccccgg ttgaagaaca tgccataaga attgctataa
     4141 tctgcatcgt ctggatcggt agctgtagaa taaacgaccg tgtgaaggaa ggaagcgaca
     4201 aggtccagat ccccatcgcc gtcgaagtcc ccggccttga tggcccccac gatctcattg
     4261 gtcgagatga aatactcgcc atacctcgat acaaagctgc ccctgttctc agaccacacc
     4321 gtgacgtcct cgttcgcggt tccagtgttc gagctgccat accaaccact ccaatgggag
     4381 gtcatgtccg cctgttggcc tgggagggcg tcgaaggcgc tgcagatgat gtacggcaat
     4441 gtggaccatt cgc
//
LOCUS       DAOSYN010000100         4406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_16238_length_4406_cov_3.064123, whole genome shotgun sequence.
ACCESSION   DAOSYN010000100 DAOSYN010000000
VERSION     DAOSYN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16238_length_4406_cov_3.064123"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PLJ11_05005"
     CDS             <1..1068
                     /locus_tag="PLJ11_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO04063.1"
                     /translation="RYPSISEGWNEDRSGRIFMGLCTPGLGPNTPMTRVWARLLMEVY
                     EQRTDPAAKYYWTLVGYFNTIRSLGNTVALYRENIIERVDELANIARRQPRSLDMTNF
                     VELHGSMDSTLVPQVLSDLEDGITKPLQQNKDAVFTTAMFGTGVDVPQLSVMLMDGQP
                     KTTSQYIQAAGRVGRNHGGLVIVFLRAGRTRDLSHYELFSSYHHRIYLDVEPPSVSPF
                     SKGAMRKAGGPTIVGYLRNIPDPRVDWATKNDGHVIVNSGADRDIADFIEALESRLSL
                     IEPMNRNEIVNYYRGQVDMWSNLSTQIAREILLMNQTLGPGSVPSNVVLGTRAHSAVQ
                     VVYPNSPRSLRDIEETTTFGV"
     gene            1074..2924
                     /locus_tag="PLJ11_05010"
     CDS             1074..2924
                     /locus_tag="PLJ11_05010"
                     /inference="COORDINATES: protein motif:HMM:NF038324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOO04064.1"
                     /translation="MGEFNQIRRSQFVFGFGPGAILETKDGSRIIPMPHIGLGKRWNR
                     SFLEQHTISDSRLSSAIGSIIGSDSRVFKLPSNDAEGMKAWETLYHVHAFPSWRICNG
                     KRSRPHEPVIYNGYSNNGVCPRCNQYEGTTPSRFIVACLDGHMDDVDWDLAVHWNMSA
                     HTCANNCTSNYYFWDAGGSSLSDIKITCPICNAQNDMSSIYSARFICTGRYPELEYVS
                     NTSREGGREGVPYSTPRTYPGCSRDMRIVQRQTSALRLPETITLLTLPKYDHPAFNIV
                     QQPAIKKSLEMLFKQYKDGLGGSSSLRDFIIQYLGNEELVKFLQDQDPDLKDFKEKID
                     GLGIAASRPFIDFMFEEFESLIGLPVGSEHFEMSHSPYVYSLKLPEKTFKLDAHQIFK
                     IRTVTAQIGYRRIPEKGLGKEPKRVSHDCNICGDPTNFYPGFEGFGEGIFIHMSDETM
                     NGELLDWLKKTGRWAEWEVTTNEDGQNQPGKLWGDVPKSPLFAWWHTLSHALIRSISI
                     YSGYSSASIRERVYIDEKNEHGGILLYTTIPGNDGTMGGLVATTEQLEPMLRNAMRSI
                     EACSNDPLCGEVRKTPERKNGAACHSCLLISETSCEQMNNSLDRHLLLGD"
     gene            2925..3386
                     /locus_tag="PLJ11_05015"
     CDS             2925..3386
                     /locus_tag="PLJ11_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOO04065.1"
                     /translation="MKVETTIVATGRNWVGNGARSFIKVVHELITESTKHLAMTIYLI
                     TSSDIVDDIESALGKGVNVDVFVCYSKIVDSQKEINELKRMREEYPNLNIFEVKDGVL
                     HAKVLISDKRRALVGSANLTPNAMKNNYELGLLVEDGGMAHNIYKILKGLD"
     gene            3386..>4406
                     /locus_tag="PLJ11_05020"
     CDS             3386..>4406
                     /locus_tag="PLJ11_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04066.1"
                     /translation="MGAYYHDLPICPTCCKSPNIAEGSYIDIPNGEGGSRICSECKVK
                     FKASINLEGRCVFHDLSILISSNGWIDPSKVPLFWRIWPNRIERDYLEWVRNPRPGNH
                     LVTWPWPEVRFAPLLAFEFLEANPKCKVLIVDSLGPNRNETDTIYSFDLVDRIGGLYK
                     TTEDIDQKSIQKDPGNKKAWHSLSKVDILEKQLTTEYYVTRAGKDHDYAVDRSRVERH
                     GIMPFRLSTCEGRIVKEIAEEVGKDRIRKVTRIKAGKSKVIKTLNPNGVYDIRLDEEE
                     RWVKDVTRTISKDDFWNIVLQRTRPRRAGDFILAAPQNNESIPAIKDDSNLIIVDISS
                     SDTDYR"
BASE COUNT         1238 a          945 c         1178 g         1045 t
ORIGIN      
        1 cgatatccat ccatctctga gggatggaac gaggaccggt caggaaggat attcatggga
       61 ctgtgtacgc ccggtctagg accgaacacc ccgatgacgc gagtgtgggc acgattgttg
      121 atggaggtct atgagcaaag gacggacccg gcagctaagt actactggac gctggtcggt
      181 tatttcaata cgattaggag ccttggaaat actgttgccc tgtatcggga aaacatcatt
      241 gaacgcgtcg atgaactggc aaatatcgca agaaggcagc ctagatcttt ggatatgacc
      301 aatttcgtcg agctccatgg atcgatggat tctaccttgg tcccccaggt ccttagcgac
      361 ttggaagacg ggataaccaa gccgttacaa cagaacaagg atgctgtgtt cacgacagcg
      421 atgttcggaa cgggggtcga tgtcccacag ctctctgtga tgctgatgga tggacaacca
      481 aagacgacat ctcagtacat ccaagctgcc ggaagggtgg ggcgcaacca tgggggactg
      541 gtcattgtgt tcttgcgtgc aggtaggaca cgagacctca gccattatga gctgttctca
      601 tcgtatcatc acaggatata tctggatgtg gaaccgccct ctgtctctcc gttctcaaag
      661 ggagccatga gaaaggcggg gggccccacc atagtgggtt atcttcggaa catcccggac
      721 cctagggttg attgggcgac gaagaatgat ggacatgtta tcgtcaattc aggagcggac
      781 cgagatatcg cagatttcat tgaggccctt gaaagccgcc tatcattaat agagccgatg
      841 aacagaaatg agatcgtaaa ttattatcgt ggacaagtgg acatgtggtc gaacttatcg
      901 acacaaattg ccagggagat actgttgatg aatcagaccc ttgggcctgg tagtgtgcca
      961 tcaaatgttg tcttgggaac acgagcgcac agtgcggtac aggtcgtata tccaaactca
     1021 ccacggtccc tacgggacat tgaagagacc accacctttg gagtgtgaag aaaatgggag
     1081 agttcaacca gattagaagg tcccaatttg tgttcggttt tggaccgggg gccattctcg
     1141 agactaagga tggttcacgc atcatcccta tgccacatat tggtctaggt aagcgctgga
     1201 atcgatcctt cctcgagcaa catacgatat cagatagcag attgtctagt gcaatcgggt
     1261 cgataattgg tagtgactcg agggtgttca aattgccctc caatgatgct gagggtatga
     1321 aggcatggga aacgttgtac catgtgcatg cgtttccttc atggaggatt tgcaatggaa
     1381 aaagaagtag gccacatgaa cccgttatct acaatggtta tagcaataac ggtgtatgcc
     1441 cccggtgcaa tcaatacgaa ggtacgaccc cttccagatt tattgtagcg tgcttggatg
     1501 ggcatatgga cgatgtcgat tgggacctcg cggtccattg gaacatgagt gcccatacat
     1561 gtgctaataa ttgtacatca aactattatt tttgggatgc tggaggaagc tcgctctcag
     1621 atatcaagat aacctgtcca atttgtaatg cacaaaatga catgagcagc atctacagcg
     1681 cccggttcat ttgtaccgga agatatccag aactggaata tgtgtccaac acgtcgcggg
     1741 aaggaggccg tgaaggcgtc ccatattcaa cacccagaac ctatcctggg tgttctcggg
     1801 acatgaggat cgtgcagaga cagaccagtg cactgaggtt accggaaacg attactctgc
     1861 tgaccttacc gaaatatgac cacccggcct ttaatatcgt tcaacagccc gccatcaaga
     1921 aaagtctaga gatgctcttt aaacaataca aggatggatt aggaggaagc agttcactac
     1981 gggatttcat cattcagtac ctaggtaatg aagagcttgt gaaattttta caagaccagg
     2041 acccggacct taaggatttc aaggagaaaa ttgatggact gggaatagct gcctcacgcc
     2101 ctttcatcga tttcatgttc gaggagttcg agtctttgat agggttgcca gtaggtagcg
     2161 agcattttga gatgtcccat agtccatatg tctattctct gaagctccct gagaaaacgt
     2221 tcaaacttga tgcgcaccaa atcttcaaga ttcgtaccgt caccgctcag ataggctatc
     2281 gtaggatacc ggagaagggt ctgggcaaag aaccaaaaag agtatctcat gactgtaata
     2341 tctgtggtga ccccaccaat ttttatccag gtttcgaagg atttggcgag gggatattca
     2401 tccatatgtc cgatgagacc atgaatggag agctgctcga ttggttgaaa aagaccggac
     2461 gatgggccga gtgggaggta acgacaaacg aggatggtca gaaccaacca ggtaagctat
     2521 ggggtgatgt acccaaatcc cctctattcg cctggtggca caccttgtcc cacgcgctga
     2581 tcaggtccat ttcgatatac tcgggctatt cgtccgcttc catcagggaa cgtgtctata
     2641 tcgatgagaa aaatgaacat ggtggcatcc tgttatacac gaccatacca gggaacgatg
     2701 gaacgatggg aggcctagta gccacaacgg aacaacttga gccgatgctc cggaacgcca
     2761 tgcggtccat tgaggcctgt tccaacgatc cgttgtgcgg agaggtcagg aagacgccag
     2821 agcgtaagaa tggagctgcc tgccatagct gtctgttaat atcagagaca tcgtgtgagc
     2881 agatgaacaa ctcattggac cgacacttgt tgttgggtga ttgaatgaag gtggagacga
     2941 caatcgttgc caccggtcgt aactgggttg ggaacggggc gaggtccttc ataaaggtcg
     3001 tccatgaatt gataacggag tccacgaaac atctggccat gaccatctat ctaataacga
     3061 gctcggacat cgtggatgat atcgagagcg cgttagggaa aggtgtgaac gtcgatgtat
     3121 ttgtctgtta cagcaagatc gtcgacagtc aaaaggaaat aaatgagcta aaaaggatga
     3181 gggaggagta tcccaacctc aacatattcg aggtcaagga tggcgtcctt catgcgaaag
     3241 tgctgatctc ggacaaaaga cgagccttgg tggggtccgc aaaccttacc ccgaacgcca
     3301 tgaagaacaa ttacgagctt ggattattgg tcgaggacgg cgggatggcg cataacatct
     3361 ataaaatctt aaaggggctt gactgatggg cgcctattat cacgacctcc ccatatgccc
     3421 cacctgttgc aagtcaccaa acatagctga gggatcgtat atcgatatcc ccaatgggga
     3481 aggcgggtcg aggatctgtt cagaatgtaa agtaaagttc aaggccagta tcaatcttga
     3541 agggcgttgt gttttccatg acctgtccat cttgatatcg agtaatggtt ggatagatcc
     3601 atcgaaagtg ccattgtttt ggaggatatg gccaaatagg atcgagagag attatcttga
     3661 atgggtcagg aaccctaggc caggaaatca tctcgtcacg tggccatggc cagaagtaag
     3721 gttcgctcct ctcctggcat ttgaatttct cgaggcgaac ccaaaatgca aagtgctgat
     3781 cgttgattca cttggaccta atcggaatga aactgacacc atctattctt tcgatcttgt
     3841 cgaccggata gggggacttt ataaaacaac tgaggatatc gatcaaaaat caattcaaaa
     3901 ggaccctggc aataaaaaag cgtggcattc cttgagtaag gtggatattt tagagaaaca
     3961 gcttactacg gaatattatg tcacccgcgc cggaaaggat catgattatg cggttgatag
     4021 gtcacgcgtc gaaaggcacg ggattatgcc atttagattg agcacttgtg aaggacggat
     4081 tgtaaaagag attgctgaag aagttggaaa agataggata aggaaggtca cgagaatcaa
     4141 ggcgggtaaa tcgaaggtaa ttaagaccct taatccgaac ggtgtctatg atattcgatt
     4201 ggacgaggaa gaacggtggg tcaaggatgt gacgagaaca atatcaaagg atgatttttg
     4261 gaatatcgtt cttcagagga caaggccaag acgtgcggga gattttatat tggcagcgcc
     4321 tcaaaataac gaatcgatcc cggcgatcaa ggatgatagc aacttgatca tcgtcgacat
     4381 ctcatcatct gacacggact acaggg
//
LOCUS       DAOSYN010000101         4354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_16462_length_4354_cov_3.711561, whole genome shotgun sequence.
ACCESSION   DAOSYN010000101 DAOSYN010000000
VERSION     DAOSYN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16462_length_4354_cov_3.711561"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..882
                     /locus_tag="PLJ11_05025"
     CDS             127..882
                     /locus_tag="PLJ11_05025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOO04067.1"
                     /translation="METWELAIILLAAIVPSLIYLIWIRNSERYNRESWMRVLSVFVF
                     GMIVSILAAMVLESVAVELLYASGSIFSGGLWSYEPYDPTVEIVVLAVVIAPLVEEWT
                     KGWGVLTVRYKVREPEDGLVYGAAAGLGFAAMENVFYNSSALLSGFDVFLSTAITRAV
                     ASTLLHATASAVVGYGIARKSLLGTRGVGASWLPYYLAAVLLHGAFNGFAVAGEVWDH
                     WAIPLIGLIAASVMAVGMFLWMRHRLKAMDRGW"
     gene            complement(857..1441)
                     /locus_tag="PLJ11_05030"
     CDS             complement(857..1441)
                     /locus_tag="PLJ11_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO04068.1"
                     /translation="MTPFVVGLNGSPRREGNSATLLGEALQGAEAHGARTARFDLAFM
                     NISPCTACDECFSFGECVLEDDMSLLYKALEEADVVIVCSPIYFSGISSYTKLAVDRC
                     QALWARRKVLCVPRRPGHGAIVLCAAQPDARFDNAVSELRAFLLGIGITPKEVMRVPG
                     AGTRTYVRESEELRASARALGRAMVDLTNPGPWP"
     gene            complement(1438..1785)
                     /locus_tag="PLJ11_05035"
     CDS             complement(1438..1785)
                     /locus_tag="PLJ11_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO04069.1"
                     /translation="MVEDNPDHTELCQEHLSPEEFDLFIARSGAECLAMAKGRPYDVI
                     LLDYMLPDTNGMDLLPQIKELQPSSLILFVSAADDPDLSYKAMTAGASDYVVKGFGYY
                     RTLRDRIREARGE"
     gene            complement(1913..>4354)
                     /locus_tag="PLJ11_05040"
     CDS             complement(1913..>4354)
                     /locus_tag="PLJ11_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOO04070.1"
                     /translation="ITEQLIRYEKEEEAFNGMSSEGKRGASGEGAGRTMVCTLDLFGR
                     LKYYNRTVEELLGTDGARLLGTPFASLLSNGSAPAFESALQECLQGRSVRERLTIELG
                     EAEFTVSMTPVKEREEVVGVSLLGFLIDPDRRQLSLGMVLDVLQDHSLLAMVYSRDGE
                     VHMVNPPLQRFLQRDRGTVEGRGISSLLQGQEDQLDKALLQPEPPSFITLPMDVQGRP
                     VKLTWSLHPFVEDGKDMVLAVADLPALPRLRAEDNESLLFLAESSADLISEGNPSEML
                     QSELDNLLHREGLDFAVVRLLGWDGRPQLFCSGVDFKQARAIFDAQVGEGTLAMRLAK
                     GEGCVVSAEDQQGGGAMGGLGAHSLICLPLRFRGDLVGAAVFGKRAAIEDLETRKLAL
                     QVLSNQMAMIYFYSLLNEHYLRACSELQALLEISRLVSSTLDHREVLDIILSKARELV
                     ESDNCIIYGLDRDGGRLVPLTYLSRYEVDPQALVVRVGEGITGDVARTGKGELVERAD
                     LDPRAKQVDGTPDEPSSLICVPLRIGDEVLGVMTLEKVPGRPFTPGEYRLMEMYAPHA
                     AMALKNSGLFQDIRSKVSAQKMYNILLTHDVANYNVPIHGFLEMLLKDPKLDERQRRY
                     VRSALAQSQNVSNLIADIRKLWNIMEDEGKVELLPVDIIPLLHAVVQEIRASPLYSDL
                     PIELQYPGESAWVLADPLVRDVLFNLISNAAKYGETRKVEVVVRPEREGDSDHWRVEV
                     RDQGKGVPDDRKPAIFQRFESLGTDVAAESHGLGLSVVKALVDRYHGRVWVEDRVKGD
                     HTKGATFVLLLPKAR"
BASE COUNT          691 a         1448 c         1419 g          796 t
ORIGIN      
        1 ccaccgtcca ggaggaacgt ctgctccctg cgttggagag gcgggacgtc aggacgggcg
       61 cgggcccggc gccagataaa tcggcacatt gatatgctcg cactcggctt aacgctcggg
      121 tgttggatgg aaacatggga gctggcaata atccttctgg cggcgatcgt cccctcgctc
      181 atctacctga tatggatccg caactccgag cggtacaacc gggagagctg gatgagagtg
      241 ctttccgtgt tcgtcttcgg aatgatcgtc tccatcctgg ccgcgatggt cctggagagt
      301 gtggcggtcg agctcctata cgcctctggc tccatcttct cgggggggct ctggtcctac
      361 gagccttatg accctacggt ggagatagtc gtcctggcgg tcgtcatcgc tcccctcgtg
      421 gaggaatgga ccaaggggtg gggcgtgctc accgtgcgct acaaggtgcg cgagccggag
      481 gacgggctcg tctacggcgc ggcggccggc ctgggcttcg ccgccatgga gaacgtgttc
      541 tacaacagct ccgccctgct cagcgggttc gacgtgttcc tgagcaccgc catcacccgg
      601 gcggtggcct ccacgctgct gcacgccacc gcctccgccg tggtcgggta cggcatcgcc
      661 cggaagagcc tgctcggaac gaggggcgta ggggcgagct ggctcccgta ctacctggcg
      721 gcggtgctcc tgcatggcgc cttcaacggg ttcgcggtgg caggggaggt ctgggaccac
      781 tgggcgatcc cgctcattgg gctgatcgcc gcctccgtca tggcggtggg catgttcctg
      841 tggatgcgtc acaggctcaa ggccatggac cggggttggt gaggtcgacc atcgcccgac
      901 cgagcgcgcg ggcggaggcc cgcagctcct cgctttccct tacgtacgtc ctggtccctg
      961 cccccggcac ccgcatgacc tccttcggcg tgatcccgat cccgagcagg aacgcccgca
     1021 gctcggagac ggcgttgtcg aaccgtgcgt cgggctgggc ggcgcacagg acgatcgccc
     1081 cgtgccccgg cctgcgaggg acgcacagga ccttgcgcct ggcccagagc gcctggcagc
     1141 ggtccaccgc gagcttcgtg tacgagctga tgccggagaa gtagatgggg gagcagacga
     1201 ttaccacgtc cgcctcctcg agcgctttgt acagaaggga catgtcgtcc tcgaggacgc
     1261 attccccgaa ggagaagcac tcgtcgcagg cggtgcaggg ggagatattc atgaaggcga
     1321 ggtcgaagcg tgcggtgcgg gcgccatgcg cttccgcccc ctgcagcgcc tccccgagga
     1381 gcgtcgccga gttgccttca cggcgggggg agccgttcag gccgacgacg aagggggtca
     1441 ttcccccctt gcctcacgga tccggtccct gagggtccgg tagtagccga agcccttcac
     1501 cacgtagtcg gaggcacccg ccgtcatggc cttgtagctg aggtcggggt cgtccgccgc
     1561 gctcacgaag aggatgaggg agctgggctg gagttccttg atctggggca ggaggtccat
     1621 cccgttggtg tcaggcagca tgtagtcaag gaggatgacg tcgtacgggc ggcccttggc
     1681 catcgcgagg cactccgccc cgctgcgcgc gatgaacaga tcgaactcct ccggggaaag
     1741 gtgctcctgg cagagctccg tgtgatcggg attgtcctcc accatgagca cggacagcct
     1801 gaccttccct attggaccac cttcgctccc gcaattgccg tccgaggtat agaactatcg
     1861 ggcacccctc tccctgatgg gtgcgaacgg ccccctcctc cgggctgcga ggctatcggg
     1921 ccttgggcag caggaggacg aacgtcgccc ccttggtgtg gtcgcccttg acccggtcct
     1981 ccacccatac cctgccgtga tagcggtcca cgagcgcctt gaccacggaa agccccaggc
     2041 cgtggctctc ggcggcgacg tcggtgccca ggctctcgaa cctctggaag atcgccggct
     2101 tcctgtcgtc cggcacgccc ttgccctggt cgcgcacctc gaccctccaa tggtcgctgt
     2161 ccccttcgcg ctccgggcgc accaccacct ccaccttcct agtctccccg tacttggccg
     2221 cgttgctgat gaggttgaag aggacgtccc ggaccaaggg gtcggcgagc acccacgcgc
     2281 tctccccggg gtactgcagc tcgatcggga ggtcggagta gaggggggag gcccggatct
     2341 cctgaacgac ggcgtggagc agggggatga tgtccaccgg cagcagctcc accttgccct
     2401 cgtcctccat gatgttccag agcttccgga tgtcggcaat gagattggag acgttctgcg
     2461 actgggcgag ggcgcttcgc acgtacctgc gctgccgctc gtccagcttg gggtccttga
     2521 ggagcatctc gaggaagccg tggatcggaa cgttatagtt ggccacgtcg tgcgtgagca
     2581 ggatgttgta catcttctgc gcggagacct tggacctgat gtcctggaag aggccggagt
     2641 tcttgagggc catggccgcg tgcggggcgt acatctccat gagcctgtac tccccggggg
     2701 tgaagggcct cccgggcacc ttctccaggg tcatcacccc cagcacctcg tcccctatcc
     2761 tcaggggcac gcagatcagg cttgacggct catccggcgt gccgtccacc tgcttggcgc
     2821 gggggtcgag gtccgccctc tccaccagct cccccttgcc cgtccgcgcc acatccccgg
     2881 tgatgccctc ccccaccctg accaccaggg cctgggggtc cacctcgtac ctgctcaggt
     2941 aggtcagggg taccaggcgg ccgccgtcgc ggtcgaggcc gtagatgatg cagttgtcgc
     3001 tctccaccag ctcccttgcc ttggagagga tgatgtccag cacctccctg tggtccaggg
     3061 tgctcgagac cagcctggat atctccaaga gcgcctggag ctccgagcat gcccgcaggt
     3121 aatgctcgtt cagcagggag tagaagtaga tcatggccat ctggttggag aggacctgaa
     3181 gggcaagctt cctggtctcg aggtcctcga tggcagccct cttgccgaag acggcggcgc
     3241 cgacgaggtc cccgcggaag cgcaggggca ggcagatgag ggaatgggcc ccgaggccgc
     3301 ccatcgcccc gccgccctgc tggtcctcgg cggagacgac gcagccctct cccttggcca
     3361 gcctcatcgc cagggtgccc tccccgacct gggcatcgaa gatcgccctc gcctgcttga
     3421 agtccacacc ggagcagaac agctgcgggc ggccgtccca gcccaataga cggaccacgg
     3481 cgaagtccag cccctcccgg tgcagcagat tgtccaactc gctctgcagc atctcgctgg
     3541 gattcccttc ggagatgagg tcggcggacg attcggccag gaacagcagc gattcgttgt
     3601 cctcggccct caggcggggg agcgctggca ggtcggccac cgccaggacc atgtccttgc
     3661 cgtcctcgac gaagggatgg agggaccagg tgagcttgac cgggcgccct tgcacgtcca
     3721 tcggcagggt gatgaaggag ggcggctcgg gctggagcaa cgctttgtcg agctggtcct
     3781 cctggccctg gaggagggag gagatccccc tcccctccac cgtccccctg tccctctgga
     3841 ggaaccgctg taggggggga ttgaccatgt gcacctcccc gtcgcgggaa tagaccatcg
     3901 ccagaaggga gtggtcctgc agcacgtcca acaccatgcc caaggagagc tggcgcctgt
     3961 cagggtctat gaggaagccg aggaggctga cccctacgac ctcctccctc tctttgaccg
     4021 gggtcatgga gaccgtgaac tcggcctccc ccagctcgat ggtcagccgc tcccggacgc
     4081 tccttccctg caagcattcc tgcagcgcgc tctcgaaggc tggggcggac ccgttcgaca
     4141 ggagggaggc gaagggtgtg cccagaagcc gggcaccgtc cgtgcccagc aattcttcta
     4201 ctgtgcgatt gtagtattta agccgaccga agaggtccaa cgtgcagacc atcgtcctgc
     4261 cggccccctc gcccgatgcc ccccttttcc cttccgagct catgccgttg aacgcctcct
     4321 cttccttttc gtaccttatc aattgttcgg ttat
//
LOCUS       DAOSYN010000102         4279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_16792_length_4279_cov_5.774858, whole genome shotgun sequence.
ACCESSION   DAOSYN010000102 DAOSYN010000000
VERSION     DAOSYN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16792_length_4279_cov_5.774858"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..467)
                     /locus_tag="PLJ11_05045"
     CDS             complement(15..467)
                     /locus_tag="PLJ11_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04071.1"
                     /translation="MSDPKQITLKQVPIYDRALLHLVRHDHARSEAVHPYEITADGLA
                     DAIGRRRSDIYGVIIYLVEQGWVEYERHRVANKRRVKTLTLTPAGRAEAARAARVVSD
                     LGGSIEDVIRAPPTMYSDANVLLNRCKVLQDQLDELRRQVESYMEGPR"
     gene            complement(464..760)
                     /locus_tag="PLJ11_05050"
     CDS             complement(464..760)
                     /locus_tag="PLJ11_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04072.1"
                     /translation="MSKLILNPEHDLYEKATNIFPKEIQGQLKDLEIMAMKILEAVLS
                     PFDDISHGPMPDQPWSTFTHRYYSRYGERLNPLVIIECDTFGDLAEREAMEAKE"
     gene            complement(748..990)
                     /locus_tag="PLJ11_05055"
     CDS             complement(748..990)
                     /locus_tag="PLJ11_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04073.1"
                     /translation="MRGALSERIDDLLTEANKLRTDIAALLQEVNDREGIEAIEGLAD
                     VPDDLEYAEALMRSVCAELELARQDAITGETIKCQN"
     gene            complement(993..1283)
                     /locus_tag="PLJ11_05060"
     CDS             complement(993..1283)
                     /locus_tag="PLJ11_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04074.1"
                     /translation="MTPIDEEYIRVVLPQLEKDIKRLSVRVEVLEAALRERGEVEARR
                     SSIEVGTPAKGGSIKCYFDPFGDPQANEDAINEAKRLLALAGGQPLPPTEVR"
     gene            complement(1507..1926)
                     /locus_tag="PLJ11_05065"
     CDS             complement(1507..1926)
                     /locus_tag="PLJ11_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04075.1"
                     /translation="MIRTVSYKASIQSDGSIYNESIEYGVEMDSMPLAQSQLDDAGAR
                     VDQLRQAILAKRREWEGVPEAPAPKVPAKPVDKAEIGQGEPGKVQEDEALLIRCVKCG
                     TVVEQKVSRKGVAYSYCPQCRDNRRKDGTEFPPTKGA"
     gene            complement(1923..2054)
                     /locus_tag="PLJ11_05070"
     CDS             complement(1923..2054)
                     /locus_tag="PLJ11_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04076.1"
                     /translation="MVTCQECQYRDHCQIYPWEECEINDWEDDGAEAIVEEEASQCQ"
     gene            complement(2278..2574)
                     /locus_tag="PLJ11_05075"
     CDS             complement(2278..2574)
                     /locus_tag="PLJ11_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04077.1"
                     /translation="MELEFSPMKYLRQMIADGREIIGEKDGHEVAVALIDGPRDGYIE
                     VYTAPAGGTLYIPVYVIDEDLQSKDSVETAIWDEVIDAIYRSATPEDWAEWEEE"
     gene            complement(2734..3009)
                     /locus_tag="PLJ11_05080"
     CDS             complement(2734..3009)
                     /locus_tag="PLJ11_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04078.1"
                     /translation="MSSKARIKCEFCGGPTTAQRLDGRVIAHVCPGCRARRYTRAGLS
                     HAQVCPVCGRVPIWVEIGQDGQLLFIHNRSSEGSRGCVVPMTDYGER"
     gene            complement(3071..3550)
                     /locus_tag="PLJ11_05085"
     CDS             complement(3071..3550)
                     /locus_tag="PLJ11_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04079.1"
                     /translation="MEQEPMVVRTFRLPRSVSERLDQMSNASGFVRQALQIAMASAEQ
                     EIINDKVAELKEAIIQAELRSQQEIARLRSALAKAEASLNSAHIQQTSSIQARLEQYQ
                     GYRRLRRNVRDARDMKGWLTGPRSVELLSECGFRDLDEAGAWMEEEYQRELKAKLVR"
     gene            complement(3795..4004)
                     /locus_tag="PLJ11_05090"
     CDS             complement(3795..4004)
                     /locus_tag="PLJ11_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04080.1"
                     /translation="MSNRQYHAYGCPRWWARVGSGDNARFLDIPKVRGDNDLRCTVEV
                     EDDVTVIYIGVGPNNKHGVRETVRC"
BASE COUNT          746 a         1342 c         1120 g         1071 t
ORIGIN      
        1 ccgcttgcat tggatcatct tggtccctcc atatatgact cgacctggcg gcgcagttca
       61 tccaattggt cctgcagcac cttgcatcga ttgagcagca catttgcgtc actatacatc
      121 gtcggtgggg cgcgtattac gtcttcgatg ctgccgccca ggtcggatac gaccctggcc
      181 gccctcgccg cctcggcacg gccagcaggg gtgagggtga gcgtcttgac cctgcgctta
      241 tttgccactc tgtgccgctc atattccacc catccctgct cgaccagata tatgatgacg
      301 ccatagatgt ccgatcgcct tctgccgatc gcgtccgcca gtccatcggc ggtgatctca
      361 tatggatgca cggcctcgga tcgcgcatga tcgtgccgga cgagatgcag gagcgccctg
      421 tcgtagatgg ggacctgctt taaggtgatc tgcttcggat cgctcattcc ttcgcctcca
      481 ttgcctccct ttccgcgaga tcgccgaatg tatcgcactc gatgatgacc aggggattga
      541 gccgctctcc atatcggcta taatatcggt gggtgaatgt gctccatggc tgatcgggca
      601 ttggcccatg ggatatgtcg tcgaatggtg agagcacggc ttctaggatc ttcatcgcca
      661 tgatctcgag gtccttgagc tgtccttgga tttcctttgg aaagatattg gtcgctttct
      721 cgtagagatc atgttctgga ttcaggatca gttttgacat ttgatcgtct cccctgtgat
      781 ggcatcctgc ctagcaagct cgagctccgc gcagacgctc ctcattagag cctcggcgta
      841 ctcgaggtcg tccgggacat ccgccaaccc ctcaatggcc tctatgccct ctcgatcgtt
      901 gacctcttgc agaagggccg caatgtccgt ccggagcttg ttcgcctcgg taagcagatc
      961 gtctatgcgt tcggataatg ctccgcgcat acctacctca cctccgtggg cgggagcggc
     1021 tgacccccgg cgagggccag aaggcgcttc gcttcgttga tggcgtcctc gttggcctgg
     1081 ggatcgccga atgggtcaaa ataacacttt atcgaaccgc ccttcgccgg cgtaccgacc
     1141 tcgatggagg accgcctggc ctcgacctct ccgcgctccc tgagggccgc ctcgaggacc
     1201 tccacgcgca cgctgagacg cttgatatcc ttctcgagct gggggaggac cacccggatg
     1261 tattcctcgt cgatcggggt cattggccgg cctccttggt cggctgatcc gcctcacact
     1321 ggaggactac ggcatagcta tggaccacgc cagactcggt caatagcgag agcgcatgca
     1381 tcatcctcat tgagatgtcg atcatgctcg agtatgattc tgccttgata taggctgggg
     1441 ggctggtggt cggtttgcca tcgttatctg tgatggtcag cttgatccct acagtatatg
     1501 gcatcctcac gcccccttgg tcggcggaaa ctcggtcccg tccttgcgcc tgttgtcgcg
     1561 gcactgaggg caatagctgt acgccacgcc cttccgtgac accttctgct cgaccaccgt
     1621 cccgcacttg acgcaccgga tcagtagcgc ttcgtcctcc tggaccttgc caggctcgcc
     1681 ttgtccgatc tccgccttgt caactggctt ggccgggacc ttcggggcag gggcctcggg
     1741 gaccccttcc cactccctgc gcttcgccag gatcgcctgc cggagctggt ccacgcgcgc
     1801 tcccgcgtcg tccagctggg actgcgccag gggcatggag tccatctcca ccccgtactc
     1861 gatgctctcg ttgtagatcg agccgtccga ttggatgctg gccttgtaag atactgttct
     1921 tatcattgac attggctcgc ctcctcctcg acgatggcct ccgctccatc gtcctcccag
     1981 tcgttgatct cgcattcctc ccagggatat atctggcagt ggtccctgta ttgacactcc
     2041 tggcatgtta ccattttagt cctccttttc gttttcttct cggtattcaa atttgaactg
     2101 acacacatga catttttgag gtttagcaac gcgtgggaac cacacgtgcc gacatctggg
     2161 gcagaccatg acgaccgatg tggactctgt gttgccctca gccgttttct cgtctgttgc
     2221 ctcttgattc attagtctcc tccgttgcga tcaataatgg cgggccgctc ggcccgccta
     2281 ctcctcctcc cactcggccc agtcctcagg ggtggcgctc ctgtagatcg cgtcgatcac
     2341 ctcatcccag atcgcagtct cgaccgagtc cttgctctgc aggtcctcgt cgatgacgta
     2401 gaccggtatg tatagggtcc ctcccgcggg tgcagtatag acctcgatat agccgtcgcg
     2461 cgggccgtcg atcagggcga ccgcgacctc gtggccgtcc ttctcgccga tgatctcgcg
     2521 accgtcggcg atcatctgtc gcagatattt cattgggcta aattccagct ccattttttt
     2581 acctcctaat actgcataca tgtatcagat atatataact tatcatgttg ctatcatcaa
     2641 tgataaaaag tgggcccggc aggatggggg gaattggagc tggagccttg ccccgccgag
     2701 ctcagtagga tattgacggc ttggatataa cctttatcgc tcgccgtaat cggtcatggg
     2761 gactacgcat cccctcgatc cctcggacga tctattgtgg atgaacagga gctggccgtc
     2821 ctgaccgatc tccacccaga tcggtacccg cccgcagacc gggcagacct gcgcatgcga
     2881 tagccccgcc ctcgtgtagc gccgcgccct gcatccaggg catacatggg cgatcaccct
     2941 gccgtccagc ctctgggcgg tcgtcggccc tccgcagaac tcgcacttga tcctggcctt
     3001 agagctcatg gtgacctatc ctagcgcccc tccccaagcc gagctggagg agggcgctgg
     3061 aggatgcggc ttagcggacc agcttggcct ttagctcgcg ctggtactcc tcctccatcc
     3121 atgccccagc ctcgtcgagg tccctaaagc cacactccga gaggagctcc acggacctcg
     3181 ggcctgtaag ccaccccttc atgtcccttg cgtccctgac gttacggcgc agtcgcctat
     3241 acccctgata ttgctctagc ctggcctgta ttgagctcgt ctgctggata tgagcgctat
     3301 tgagcgatgc ctctgccttg gccagggcgg acctgagccg cgcgatctcc tgttgcgacc
     3361 tgagctccgc ctgtatgatg gcctccttga gctctgccac cttatcgttg atgatctcct
     3421 gctcggcgct ggccattgcg atctgcagcg cctgcctcac gaacccggat gcattggaca
     3481 tctgatccag gcgctcgctc acgcttcggg gcaagcgaaa tgtcctgacg accatgggtt
     3541 cttgttccat ggacctcctg gtcaatacga tccctccact gggttctcat gaaggagccg
     3601 gcgctggcaa tatatgcacc tccgcgggcg gtccaccctt ggcgtccagg atagtccaca
     3661 ataaggacat gtgatcgccg gtcttggctg gcgcaccagc ttatccagtc ggtccttgca
     3721 tctcgggcat tccctgggat ggtcggtcct cgaggtccac tcgtagccgc aatgcccgca
     3781 gcggaattgc atacctagca ccgcaccgtc tcgcggaccc cgtgcttgtt gttcggcccg
     3841 acgccgatgt agatcacagt cacgtcgtcc tcgacctcga cagtgcatct caggtcattg
     3901 tctccccgca ccttggggat atcgaggaac ctggcgttat ccccgctgcc taccctagcc
     3961 caccatcggg ggcatccata ggcatggtat tgacgattgc tcatgcttgg gcccttgttc
     4021 ctgatctcta cggtcttcaa tcttgacacc tctaattagt agtatgtcgt tgttagtata
     4081 tataacttat ggacttgtta tcattattga ttttcgtgcg atatctagct ctaaagatct
     4141 atgcctagtg ataacgtatt acgtaataca ttaacgtatt acgtattacg aaaacgtctt
     4201 ctatactctc tctcccgttc cacaggaaac ggttccgatt ccaatatata aatctaatta
     4261 tgtataacat tattcgagt
//
LOCUS       DAOSYN010000103         4255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_16898_length_4255_cov_4.548333, whole genome shotgun sequence.
ACCESSION   DAOSYN010000103 DAOSYN010000000
VERSION     DAOSYN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16898_length_4255_cov_4.548333"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..780
                     /locus_tag="PLJ11_05095"
     CDS             301..780
                     /locus_tag="PLJ11_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04081.1"
                     /translation="MGPSPVQSPSAPQAMGPSQGTMLDPGVASAAMLRPNERAVHAWR
                     AGMERTGAVGDAVGGGSVVYGVLVATDQRLIFVQDRGTLADRYVLLESVEYGGIADWR
                     LSETTGVRALQVDSRDRGPWGNFVNLYEVDPMTLRGIVPGPLEQLRRSLGALKGTMP"
     gene            complement(959..1507)
                     /locus_tag="PLJ11_05100"
     CDS             complement(959..1507)
                     /locus_tag="PLJ11_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOO04082.1"
                     /translation="MDRAGALELVKAHVSKEGNLRHMVAVGAVMRQVALHLGEDPEAW
                     EVIGILHDIDIEECHGREDHTLIARDILRGMVPPEMVQTILAHNFEASGVPVDDQAKR
                     ALVAADAVSGLVMACALVLPSRRLTDLRVSSISKKFGNKDFARGVDRERIVVCEQLGI
                     PRDELLSLALEGMRARAEDLGL"
     gene            complement(1539..1943)
                     /locus_tag="PLJ11_05105"
     CDS             complement(1539..1943)
                     /locus_tag="PLJ11_05105"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOO04083.1"
                     /translation="MVKMPPEVKETLAKQKPIPIATATKDGTPNVVFVGLLKVLDDET
                     LMLADNYFLKTAANLSENPRISILCYDSETKRSYQIKGSAKVYKAGPQFEEMRKWVHG
                     TNPKLPAKAAVVVKVEAVYDSMWGPSAGKLIV"
     gene            2039..2569
                     /locus_tag="PLJ11_05110"
     CDS             2039..2569
                     /locus_tag="PLJ11_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOO04084.1"
                     /translation="MSNKEAVLMVGRLMELAARTAPKAMGQDFIEASLLSDEQRVRLG
                     EDMIALGRERGVPGFGRDGQNVLDSDAVVLLGLLPHLGAGLDCGACGLSSCQEFNSRS
                     AAKDFQGPNCALRLLDLGIALGSAAKVAAEHNVDNRIMYRIGVSAIRLGLSRANVCLG
                     IPLSASGKNIFFDRGR"
     gene            complement(2570..3136)
                     /locus_tag="PLJ11_05115"
     CDS             complement(2570..3136)
                     /locus_tag="PLJ11_05115"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOO04085.1"
                     /translation="MGKATLGRKVPTMGLPVAVVGAKVQGKVNFCTVAWVTMVEDDPP
                     RMAVVMGKRRRTKDGIRENRTFSINLPGVEQVAATDYCGLRSGYETDKGEVFDVFFGK
                     TLTAPMARECPVNIELTLDDIIELEGTDIVIGEVEEVYVDEGRMTSGQPDIDKLQLLM
                     YLSPGGPYFVKGRTVAEAFQVGKGFRPR"
     gene            3281..3913
                     /locus_tag="PLJ11_05120"
     CDS             3281..3913
                     /locus_tag="PLJ11_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04086.1"
                     /translation="MAEAKPDALPFAADALHTTALAASPDAALIEGEAGYYGLSRAVL
                     EIGRSCLTPGARVVDLRCDLRVVMPLIEEHEDLCRFTLLSPDEESYLQCFDRMRTRVR
                     LGFVDTAQIDLAERFPEVSARLFLALGALGDLSEGRRAEVARSMHRRLERGGTGVIME
                     TVEDGIALGQRRKRGLWSAAEWESLFKHAGFSNVQRLWTDGGRMAWSVRK"
     gene            complement(3957..>4255)
                     /locus_tag="PLJ11_05125"
     CDS             complement(3957..>4255)
                     /locus_tag="PLJ11_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HOO04087.1"
                     /translation="RGKTARVYGQLTALLTLTVLFTTDKKAAAGFAGIPIGIVLALIH
                     LISIPVDNTSVNPARSIGPALFQGGWALEQLWIFIAAPMVGAALAAIIYDLLKD"
BASE COUNT          719 a         1401 c         1334 g          801 t
ORIGIN      
        1 ccatcggcca ccaaccccca gcctccgccc cagtggcagg tcccttcatc gttcccggcc
       61 ccgggccccc cgcccgatcc ctctgggccg tcataccagc cgtcgggtcc ggggccatac
      121 tggccatctc cgccggaccg ccctgccccc gcggcatcgc cgcggtctcc ctcaccggcc
      181 cccagccccc catcgcggcc gtatcagggg ccgccctcgc agtggacgat gcctccccag
      241 gcgccttatg ctggcccgat cgtcgaacag cccccgcagc cttctcgatc cggccctccg
      301 atgggcccat ccccggtcca gagcccatcc gcaccacagg cgatgggacc gtcccagggg
      361 acgatgctcg accctggcgt ggcctccgcc gccatgcttc gtccgaacga aagggcggtg
      421 catgcatgga gggcgggaat ggagcggacc ggagcggtcg gtgatgcggt cggcggaggg
      481 tccgtggtct atggggtgct cgtagccacg gaccaacgcc tcatcttcgt ccaggacagg
      541 gggacgctcg ccgaccgcta tgtgttgctg gagagcgttg agtacggggg catcgcagat
      601 tggaggctca gcgagacgac gggggtcagg gcactgcagg tggactcccg ggaccgcggc
      661 ccctggggga acttcgttaa cctctatgag gtggacccga tgaccctgag gggcatagtg
      721 cctgggccct tggagcagct caggcgatcg ctgggagcgc tcaaggggac catgccttga
      781 accttcgcgc atcgtagccg ttgatcacag ctgcatgacc cctgagcaca atcgggtgca
      841 tcattgatgt tgcccatcgg tccggccggc agtgctgacc ccgtgctcct cggtccgtgc
      901 agctcgttcc tagaccgacc cgtccatcag gggcctgcga tgaaaaaaaa aggacacctc
      961 aaaggccgag gtcctccgcc ctcgccctca tcccctccag cgccaacgag agaagctcgt
     1021 ccctagggat tccgagctgt tcgcagacca cgatgcgctc ccggtccacg ccgcgggcga
     1081 agtccttgtt gccgaacttc ttggagatgg agcttaccct caggtcagtg agccttctgc
     1141 tcggaaggac gagcgcgcat gccatgacca acccggacac ggcgtccgcg gccacaagcg
     1201 ccctcttcgc ttgatcgtcg acaggcaccc cgcttgcctc gaaattgtgg gcaaggatcg
     1261 tctggaccat ctcgggcggg accatccccc gcaggatgtc cctggcgatc agcgtgtggt
     1321 cctccctgcc gtggcactcc tcgatgtcta tgtcgtgcag tatgccaatg acctcccacg
     1381 cctcggggtc ctccccgagg tgcagggcga cctgcctcat caccgcccca accgccacca
     1441 tgtgccgcag gttgccctcc ttgcttacgt gggccttcac cagctcgagc gctcccgccc
     1501 tgtccatgcc ggccctcccc gcccccaggt cgctcgcctt agactatcag cttaccggcg
     1561 gaggggcccc acatggagtc atagacggcc tccaccttga cgacgaccgc ggccttggcc
     1621 gggagcttgg ggttcgtccc gtgcacccac ttgcgcatct cttcgaactg cggcccggcc
     1681 ttgtagacct tggccgagcc cttgatctgg tacgaccgct tggtctcgct gtcatagcac
     1741 aggatggaga tgcgggggtt ctccgagagg ttggcagcgg tcttcaggaa gtagttgtcc
     1801 gccagcatca gggtctcgtc gtcaaggacc ttcagcagtc ccacgaagac cacgttgggc
     1861 gtcccgtcct tcgtagcggt ggcgatcgga atgggcttct gcttggctag cgtctccttg
     1921 acctcaggcg gcatcttgac catttttatc accgtgatag gaattatttg ctcacctatt
     1981 taaacatcgc cgcccggggg atgaaagggt catatgctcg cacgtccaat cagttggcat
     2041 gagcaacaag gaagccgtgc tgatggtcgg ccgactgatg gagctggccg ccaggaccgc
     2101 gcccaaggcc atgggccagg acttcatcga ggcctccctg ctctccgacg agcagcgagt
     2161 caggttaggc gaggacatga tcgccctggg cagggaaagg ggggtcccgg ggttcgggag
     2221 ggacggacag aacgtcctcg attcggatgc ggtggtgctc ctcggcctgc tcccccatct
     2281 gggagcgggc ctggactgcg gggcctgcgg cctttccagc tgtcaggagt tcaattcccg
     2341 ctccgccgcg aaggacttcc agggaccgaa ctgtgccctc aggctccttg atctgggaat
     2401 agcccttggt tcggcagcga aggtggccgc cgagcacaac gtggacaacc gcatcatgta
     2461 ccgcatcggt gtctccgcca tccgcctggg gctctcccgg gcgaacgtat gtctaggcat
     2521 accgctctcg gcgtccggca agaacatctt cttcgaccga ggccgctgat cacctcggcc
     2581 gaaagccctt gccgacctgg aacgcctcgg cgaccgtccg ccctttgacg aagtaggggc
     2641 cgccgggact gaggtacatc aacagctgga gcttgtcgat gtccggttgc cctgaggtca
     2701 tgcgcccctc gtccacgtag acctcctcca cctctccgat cacgatgtcc gtgccctcga
     2761 gctcgatgat gtcgtcgagg gtcagctcga tgttgacggg gcactccctg gccatgggcg
     2821 cggtgagcgt tttgccgaag aagacgtcga acacttcccc cttatccgtt tcatatccgg
     2881 agcggagccc acagtaatcg gtggcggcca cctgctccac gcccggcagg ttgatgctga
     2941 aggtcctgtt ctcccttatc ccgtccttcg tgcggcgcct cttccccatc acaactgcca
     3001 tcctgggagg gtcgtcctcg accatcgtga cccatgccac ggtgcaaaag ttcaccttcc
     3061 cttggacctt cgcccccacc acggccactg gcagccccat ggtggggacc ttcctaccca
     3121 atgtcgcctt gcccatagct atcgaatggg aattgcctcc gggaatatca atcctggccc
     3181 ccttttcccc ctttttgggg gaggaacaaa acgcatatat atttccatgc tatgtcatga
     3241 catagcacgt taaccgtgca tataggaagg aggataggaa atggccgaag cgaagcccga
     3301 tgccctgccg ttcgcggcag atgccctgca caccaccgct ttggcagcgt ccccggacgc
     3361 tgcgctgatc gagggcgagg cgggctacta tggcctttcc cgcgcagtcc tggagatagg
     3421 gcgctcatgc ctcacccctg gcgccagggt ggtggacctg cgctgtgacc tacgagtggt
     3481 gatgccactg atcgaggagc atgaggacct gtgccgcttc accctcctgt ccccggacga
     3541 ggagagctac ctccaatgct ttgacaggat gcgcacgagg gtgcgcctgg ggttcgtgga
     3601 caccgcccag atcgatttgg ccgagcggtt ccccgaggtg tcggccaggt tgttcctggc
     3661 cttgggagcc ttgggggacc tgtcggaggg gcgccgggcg gaggtcgcga ggtccatgca
     3721 tcgccggctg gagaggggcg ggaccggcgt gataatggag actgtcgagg acggcatcgc
     3781 gctggggcag cgccgtaaga ggggtctgtg gtcggcggcc gaatgggaat ccctcttcaa
     3841 gcatgccggg ttcagtaacg ttcagaggct ttggaccgac ggcggcagga tggcctggtc
     3901 agtaagaaaa taggaagcaa tgagggggct tgggccccct cgagatcgtt ttccactcag
     3961 tccttaagca gatcgtagat gatggcggcg agagcggcac cgaccatggg ggcggcgatg
     4021 aatatccaga gctgctccag cgcccatccg ccctggaaga gggccgggcc gatcgaacgg
     4081 gccgggttga ccgaggtgtt gtccaccggt atggatatca gatggatcag ggccagcacg
     4141 ataccgatcg ggatccccgc gaatccggcg gcggccttct tgtcggtggt gaacaggacg
     4201 gtcagcgtca gcagggcggt gagctggcca tagacgcgcg cggtcttgcc gcgcg
//
LOCUS       DAOSYN010000104         4222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_17070_length_4222_cov_5.078474, whole genome shotgun sequence.
ACCESSION   DAOSYN010000104 DAOSYN010000000
VERSION     DAOSYN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17070_length_4222_cov_5.078474"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..571
                     /locus_tag="PLJ11_05130"
     CDS             137..571
                     /locus_tag="PLJ11_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO04088.1"
                     /translation="MTEGERRNPNFIEVSHGRLRVNVPLSAYEGTTYHIKPEEAEKLK
                     RTLASRYPWLTPNALKVFIEEAEQAMRERIQSQLGEMERARHALQAGGYERAKRMAED
                     YLEDRPDDPDVWYVLGEALCRLGDTEAGFAALRKARALSGKG"
     gene            complement(575..1420)
                     /locus_tag="PLJ11_05135"
     CDS             complement(575..1420)
                     /locus_tag="PLJ11_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04089.1"
                     /translation="MPNLPDHCKDVSLRHVDFPLTRENILSSFQGKVAYTRTDFMVLR
                     NGEDTATVRVIKRGGKDLFRPIIDLEILSLPEDTVFIHDEGIDVLNMSQLARLVRENG
                     GRTVVVSGMFSHVSFLKAERLLDLRVLDVVPPSPSKLSVLVERALSSGLVERPIIPSY
                     VDVDIMEKERMVRTPGVIFPCRASGLSSEKRMFYLDETPTIDVESTLIGCDLSRRIFQ
                     HIYRRPIEFLDICPQNLAPRDGVPTIVKCCKVKEGCVIEGSVASVPWGATMMDVVQAL
                     QALFP"
     gene            1604..2641
                     /locus_tag="PLJ11_05140"
     CDS             1604..2641
                     /locus_tag="PLJ11_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOO04090.1"
                     /translation="MARGAYRIEINYSKNGRPRYFLVKDIVVKGKKRKVLKYIKTGDA
                     PSPDELDRFKREHAYQLELKAARKKAELSAEHYSTGFLSKDIIVQLEELRFIYLRFEE
                     LLTVNEAEIYERDFEVRYVSGTTGYEGNTLSLEQTKDLLVSGILPRDKASREIYEVDN
                     FKRVIRYRNTYRKKVSLDFIRNLHALIMDNIDNESAGTFRRTDDVIIGGCSMDLTPAA
                     VIEDELAQAIEKYYSDLKEGRHPFESAIMFHYTFETIHPFTDGNGRVGREILNYMLKK
                     ERFPKLLFLGRDRDMYISALRMGNEERYGDMVSSMAELILSQRMEILRRNLEKVIEPP
                     KKSGQMRLTDF"
     gene            2765..2853
                     /locus_tag="PLJ11_05145"
     tRNA            join(2765..2800,2816..2853)
                     /locus_tag="PLJ11_05145"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2797..2799,aa:Cys,seq:gca)
     gene            complement(2855..3286)
                     /locus_tag="PLJ11_05150"
     CDS             complement(2855..3286)
                     /locus_tag="PLJ11_05150"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOO04091.1"
                     /translation="MKEIKGSVMVNAPASKVWERVIDFPSYPTWNPFIVQMKGELKEG
                     SAFEVVISTPSKKEIRLRSRAVKVENGKEALFKGTVRKGLLSMDHSFLVEGLEDAKCI
                     LTQHIALRGLLSFFSRATARDLQAGLGNMNAEVKRLCEKKE"
     gene            3620..>4222
                     /locus_tag="PLJ11_05155"
     CDS             3620..>4222
                     /locus_tag="PLJ11_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04092.1"
                     /translation="MAKLKFIRGTPRALYRSALADFVDAFRAAPLDTALVVPRPGMAM
                     EVRATVLRGMVVPTFCVTDLDDLVRYLFDQNEKGLRPVGGWAAKRVIRQVLLGNEARF
                     QPLVRDGEVSEETLDGLYTLAATLRDFHADLGRFREDGGMGIDVPLFLRLYLERMASL
                     GLVDPMGGRGTVTGRLGEWYRAAPLFRRLVVIGPFEPTPSQ"
BASE COUNT          916 a         1177 c         1298 g          831 t
ORIGIN      
        1 aagcttgggg ttgaaagggc taagggccac ctcgttcatg gttgttgcat tcgatcgaag
       61 gttcaaaatt gtttccatgg gccgcccccg gcattcggcc ggtagctatt tcaatgggct
      121 gacggttgcg agcggcatga cggaagggga gaggcggaac cccaacttca tcgaggtcag
      181 ccacgggcgg ctccgggtga acgtgcccct ctccgcgtac gaagggacca cctatcacat
      241 caagcctgag gaggctgaga aattgaagag gacgctggcc tcccgctatc cctggctgac
      301 cccgaacgcg ctcaaggtgt tcatagagga agcggagcag gcgatgcgcg agcgcatcca
      361 gtcgcagctg ggggagatgg agagggccag gcacgccctg caggccgggg gctacgagcg
      421 ggcgaagcgg atggccgagg actatctgga ggacaggccg gacgaccctg acgtatggta
      481 cgtgctcggg gaggccctct gcaggctggg cgacacggag gccgggttcg ccgccctcag
      541 gaaggcgcgc gccctgagcg ggaagggctg agcgtcaggg gaagagcgcc tgcagcgcct
      601 gcaccacgtc catcatggtc gcgccccatg gcacggaggc cacgctgccc tcgatgacgc
      661 acccctcctt caccttgcag catttgacga tggtcgggac gccgtccctt ggggcaaggt
      721 tctgcgggca gatgtccagg aattcgatgg gacggcggta gatgtgctgg aagatgcggc
      781 gggagaggtc gcagccgatg agcgtcgatt caacgtcgat cgtcggggtc tcatccaggt
      841 agaacatcct cttttccgaa ctcaggccgg aggcccggca cgggaatatc acaccaggcg
      901 tcctgaccat ccgctccttc tccatgatgt cgacgtccac gtacgagggg atgatcggcc
      961 tctcgaccag gccggaggag agcgccctct ccaccagtac cgagagcttg gacggggacg
     1021 gagggacgac atcgagaacg cggaggtcca gcaggcgctc ggccttcagg aaggagacgt
     1081 gggagaacat gccggagaca acaacggtcc tgcccccgtt ctccctgacc agtctggcca
     1141 gctgcgacat gttgaggacg tctatcccct cgtcgtggat gaagacggtg tcctccggga
     1201 gggataggat ctccagatcg atgatcggcc ggaagaggtc cttacctccc cgtttgatca
     1261 cccgcaccgt tgccgtgtcc tcgccgttcc tcaggaccat gaagtccgtg cgggtgtagg
     1321 cgaccttgcc ctggaagctg gagagtatgt tctcccgcgt cagggggaag tccacgtggc
     1381 gcagggagac gtccttgcag tggtcgggga ggttcggcat gcgcgtttct atggtctgtg
     1441 cctatttaat caaattgcct tcatctcccc agccgcatct aggcgcgatc aagggcgttc
     1501 ttcagtaaag gcatggcata atggaacgga ccgattaatt atataatagg tgaccattat
     1561 aaaaatcaca ttttaataac aatggtcgcc tactcatact tcaatggcta ggggcgcgta
     1621 tcggatcgag atcaactact ctaagaatgg caggccaagg tactttttgg tcaaggacat
     1681 cgtcgtgaaa ggaaagaaaa ggaaggttct gaaatatatc aagacaggcg atgccccgtc
     1741 tcccgatgag cttgacaggt tcaagcgcga acatgcgtat cagcttgaat tgaaggcagc
     1801 caggaagaag gcggagctta gcgcggagca ctacagtacg ggttttctaa gcaaggatat
     1861 catcgttcag ctcgaagagc tgcggttcat ctatcttcgg ttcgaagagc tattgaccgt
     1921 caatgaggcc gagatctatg agcgcgactt cgaggtgcgt tatgtttctg gaacgacagg
     1981 atacgagggc aatacgctct cattggaaca gacaaaggac ctattggtgt ccgggatcct
     2041 tcccagggat aaggcatcga gggagatcta tgaggtggat aacttcaaga gggtcatccg
     2101 ttaccgcaac acatatagga aaaaggtcag cctggacttc atccggaacc tgcacgcgct
     2161 tataatggat aatatcgaca acgaatcggc cggaacattc cggaggaccg atgacgtgat
     2221 catcgggggc tgctcaatgg acctgacccc ggcggctgtg atagaggatg agctggctca
     2281 ggcgatagaa aagtactatt ccgatctgaa ggaaggtagg catccctttg aatccgccat
     2341 catgtttcat tatacgttcg aaacgattca ccctttcacg gacggcaatg gccgggtggg
     2401 aagggagatc ctgaactata tgctcaagaa ggaaaggttt cccaagctgt tgttcctggg
     2461 gagggataga gatatgtaca tttccgcatt aaggatggga aatgaggagc gctacggaga
     2521 catggtctcg tcgatggccg agcttatcct ttcccaaagg atggaaatat tgagaaggaa
     2581 cctcgagaag gtgatcgagc ctccaaaaaa gtccggacag atgaggctga cggatttcta
     2641 aagacgttag gaagggataa cccatctccc gatcatctga ccattatggc caccccattc
     2701 accagagagc acatccaggc agaaggaaaa cctaattata cggggtacag aatcagccat
     2761 caaggctggg atgacagagc ggccatgtgg cggactgcag tccaccaagg cggttatccg
     2821 tacacccgtg ttcgaatcac ggtcccagct ccattcactc cttcttctcg caaaggcgct
     2881 tcacctccgc gttcatgttg cccagccccg cctggaggtc gcgagcggtc gcccttgaga
     2941 aaaaggacag gaggccgcgg agggcgatgt gctgcgtaag gatgcacttg gcgtcctcca
     3001 gcccctcgac gaggaacgaa tggtccatgg aaagcagccc cttccggacg gtccctttga
     3061 acagggcctc cttcccattc tcgaccttga ccgccctgga cctgagcctg atctccttct
     3121 tgctgggggt tgatatgacc acctcgaagg cgctcccctc cttgagctcc cccttcatct
     3181 ggacgatgaa gggattccaa gtgggatagg acgggaagtc gatcaccctt tcccagacct
     3241 tcgatgccgg cgcattgacc atgaccgagc ccttgatctc cttcatggga tagcgtaccg
     3301 ccttcgggct gatataatgc ttaatattaa tttaataatt gaataatata ccgcccgatg
     3361 acccgtcgga taagggcagc aggtcatacc cttccccaat aggccggtcc cgtcatccgc
     3421 ctgcgggcgt cgccgtcctc tttggaggga gcgcgtgccc gaagcacgaa gggtgaacag
     3481 gagcgcatat atgcggcttt cataggacgg tattagggac gatgggaccg atgcccttcg
     3541 cgaactcgat cccccggccc tcttccagtg gcatcacctg agggaaaacc tttttcaagt
     3601 gaggggacag gttccgtccg tggccaagct caagttcatc agggggacgc cgagggcgct
     3661 gtaccggtca gctctggccg atttcgtgga cgccttcagg gccgcccccc tggacaccgc
     3721 cctggtggtc ccccgccccg gtatggccat ggaggtccgc gccaccgtgc tgagggggat
     3781 ggtcgtcccc accttctgcg tcaccgatct ggacgacctc gtccgttacc tgttcgacca
     3841 gaacgagaag gggctgcgac cggtgggcgg atgggcggcg aaaagggtca tccgccaggt
     3901 gcttctcggg aacgaggcgc gctttcagcc cctcgtccgg gacggagagg tttccgagga
     3961 aacgctggac ggcctctaca ccctggccgc gacgcttcgg gacttccacg ccgacctggg
     4021 ccgcttccgc gaggatggag gcatggggat cgacgtcccc ctcttcctca ggctgtacct
     4081 ggagaggatg gcctccctcg gcctcgttga cccaatgggg ggacggggga ccgtgaccgg
     4141 aaggctgggg gaatggtacc gcgcggcgcc gctcttcagg aggctggtgg tcataggccc
     4201 cttcgagccc accccctccc ag
//
LOCUS       DAOSYN010000105         4179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_17278_length_4179_cov_10.815228, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000105 DAOSYN010000000
VERSION     DAOSYN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17278_length_4179_cov_10.815228"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            907..1107
                     /locus_tag="PLJ11_05160"
     CDS             907..1107
                     /locus_tag="PLJ11_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04093.1"
                     /translation="MTHYEIWTAPYKGENALRHEYHRHGEDLDKVIAEARSLARTERD
                     VCVVEISEEGDRHATVMCRIES"
     gene            complement(1737..3164)
                     /locus_tag="PLJ11_05165"
     CDS             complement(1737..3164)
                     /locus_tag="PLJ11_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04094.1"
                     /translation="MTSTKKAKDLSDKVEGAELFVKEDGALGAVLPHYAPDHIRPLYE
                     WRQEGDGIAEYLDEQLSNAFWPICGAPTTGEDPALFEDLERFIRDHVYFQDEAIYETV
                     TSWAICTWIKEALRIAPRPIFYGTTASGKSRAINTLREVVYRGYVPTIPTPAVLYALI
                     ERYQPTIFIDEYQAIKNPEKLNDIEAVFRAGYERGGTVPRMNPNTMEPIIHHVYGFMG
                     IGTKGRLPPEDLVNRALIVNMQQKPKEAAIARRIDDARGQALRGRLLGLRLRVLSGDV
                     SIAAERDRAVAEALRPVMHRGASTELGDRPVDLAESLLVPPLLFGRPCGILGVIARCQ
                     AESRDELVGTLEGRTFHALQACYRGCPPLKTGTLEGQPLRDIAQLTTLNVADQLNLDL
                     MEQEGRDTYTKRTRIETHTVTSWLKTLGFSFRRGTGNRSYFLDDGFMRAYRTNLDKYG
                     VREGCPDIRGRQLTIADEVNLGPRS"
     gene            complement(3184..3498)
                     /locus_tag="PLJ11_05170"
     CDS             complement(3184..3498)
                     /locus_tag="PLJ11_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04095.1"
                     /translation="MRKIVRESGELYLLDTAKDLLVFNGRKHQGARQDRWLELWLHRT
                     EGAAIYYVAHMTRWPGETSYLEAVSAEQAADLVGGNIDRLSDEDLRSVERLGLIDLSA
                     LR"
     gene            3648..3812
                     /locus_tag="PLJ11_05175"
     CDS             3648..3812
                     /locus_tag="PLJ11_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOO04096.1"
                     /translation="MNESRQATKKICISIDKELLDWIDAQVRERRFATRSHGIALAVQ
                     ELGRKEQGSA"
BASE COUNT          823 a         1279 c         1321 g          756 t
ORIGIN      
        1 agcgagtttt gtcagcctat agcgatcccg gcacgggtcc cgcggccctc acgcgctgaa
       61 tctctctttt tggggtttcc aggaatgtcc aatagcgatc ccggcacggg tcccgcggcc
      121 ctcacgcggg ggcgagttaa ctccttctca tgttaactcg cagttaactc gcagttaact
      181 cggcgatctc tcctcatttt gaggacagcc agcgtgtctg agggcgatcg ccgccgaatg
      241 aagatagtta attaagttaa ttaagttaac aaataaggaa ggatagaggg attcttctac
      301 ggcgcgcgga ccttcgcaca cgccagcaac aaagaggggc gcgaaggcgg tcaggagcga
      361 tctagaggcg tcctcagcgt caaacgaacg cccccatcga ccaagggaag aacgcgccta
      421 caaggcccgg ttccgaggcc ggtcctcctg gcatcgggcc tgacggttcg gacatcctcc
      481 ccggtcgcag ggagcggccc gccgagggcc ctagagcggc cctagaacga tcccgaatcg
      541 gtgcaggccc ggtcccagac cggccccggg ccaaaccgga cctgggatcg gggccgatcc
      601 ggacctagat ggggccggac ctgctccata atcggagcat ttccgagagg atataggatg
      661 gagcgccaag gtacatatac cgaaccagac gataccgata cgtgcacggc gagccgccaa
      721 ggagccacac aaggctcgaa gttaaacgaa aaccccgatg ccctaaatgc gatacggtgc
      781 tcatccagtt atatcgacag cgggggaagt tcaacagcac cacggggcac acatgggaga
      841 aggtggcgct gtattgcgag gagtgcaagg ctctccgggc cccaccgatc gaacggggga
      901 acgctcatga cgcactatga gatctggacc gctccctata agggggagaa tgcattgagg
      961 cacgaatacc accggcacgg agaggacctc gacaaagtaa tagccgaggc gcgatcgctc
     1021 gcgaggaccg aacgggacgt ctgcgtggtg gagatctcgg aggaggggga ccggcacgcc
     1081 acggtgatgt gccgtataga gtcataaaat tggcattgaa tgccctcctc ggggcgccct
     1141 cggggcgtgc cgcatgggtg aggcggccgg ggccgggatc gagggatgat gggggggtcc
     1201 gcggctcgcc tggacggaga tcacggcctg aggcgcgggg tgaggcgttg agggggggtg
     1261 gggcggtcct accaatgtaa catattgtcc gaaggaccgt aggtatgtag gatgaatgat
     1321 gatgagaaag gctggctagg accaactaag agtttgtccg aaggactgta ggtaggtagg
     1381 atgaataata ggaggatgga aggccgccgc ccccccaaag gtccttggac catctgtcga
     1441 gctctcgaca ggggggagct gtcggaatac ctccccccga aagaccgccc cctgttctta
     1501 tagtccgagg aggtcccgcc gatgacggag gacgggcgtg atccgacacc cccgagcttt
     1561 taccgagctt taccctaggt cccgcaggcc gtcctgcggg cctctagcgg cgctcctagc
     1621 gcccctagcg gcgccggcgc ccgccccccc cttgaaaatg ttaacttaat taacttaatt
     1681 aactccctcg gagcgctggc tcgggccccg ggaacagtta actcggcggc ccgaagttaa
     1741 ctcctcggtc ctaagttaac ttcgtccgca atggtcagct gccgccccct gatgtccggg
     1801 cagccctcgc ggaccccgta cttgtctagg ttcgtccggt acgcccgcat gaacccatca
     1861 tccaggaaat aggaccggtt gcccgtcccc cgcctgaagg agaaccccag ggtcttgagc
     1921 cacgaggtca cggtgtgcgt ctcgatgcgg gtccgcttgg tgtaggtgtc ccggccctcc
     1981 tgctccataa ggtccaggtt gagctggtcg gccacgttca gcgtggtgag ctgggcgatg
     2041 tcgcgtaggg gctggccctc cagggtgccg gtcttgagag gagggcagcc ccggtagcaa
     2101 gcctggaggg catggaaggt gcgcccctcc agggtgccga ccagctcgtc cctcgattcg
     2161 gcctggcatc tggcgatgac ccccaggatc ccgcacggcc gaccgaacag gagcggcggg
     2221 acgagcaggc tctcggccag gtcgacgggc cggtccccca gctcggtgct cgccccgcgg
     2281 tgcatgacgg gccgcagggc ctcggcgacg gcgcggtccc gctcggcggc tatggacacg
     2341 tcgcccgaga ggaccctcag cctgaggccg agcaggcggc cccggagggc ctgcccgcgt
     2401 gcatcgtcga tcctccgcgc gatggccgcc tccttcggct tctgttgcat attgacgatc
     2461 agcgcccggt tgaccaggtc ctccggcggc aatcgcccct tcgtgcctat gcccatgaac
     2521 ccgtagacgt gatggatgat cggctccatc gtgttggggt tcatgcgggg gaccgtgccc
     2581 ccccgctcat atccggcccg gaacaccgcc tcgatgtcgt tcagcttctc cgggttcttg
     2641 atggcctggt attcgtctat gaagatcgtt ggctggtagc gctcgatcag cgcgtacagg
     2701 accgcgggcg tcgggatcgt cggcacgtac ccgcgataga ccacttcgcg cagggtgttg
     2761 atggccctgg acttgccgga ggccgtggtc ccgtagaaga tggggcgagg ggcgatccgg
     2821 agcgcctcct ttatccaggt gcatatggcc caggaggtga cggtctcata gatcgcctca
     2881 tcctggaaat agacatgatc gcggatgaac cgctcgaggt cctcgaacag cgcagggtcc
     2941 tctccggtcg tcggtgcgcc gcagatcggc cagaaggcgt tgctcagctg ctcgtccagg
     3001 tactccgcga tcccgtcccc ctcctgcctc cactcgtaga gcgggcggat gtggtcgggg
     3061 gcgtaatgag ggaggaccgc cccaagcgcc ccatcctcct tgacgaagag ctccgctccc
     3121 tcgaccttgt cggaaaggtc cttggccttc ttcgtgctag tcatcggacc accaggcgct
     3181 ccctcaccta agggccgata ggtcgatcag cccgagcctc tccacggacc gcaggtcctc
     3241 atcgctcagc cggtcgatgt tcccccccac gaggtcggcg gcctgctcgg cgctgaccgc
     3301 ctccagatag gaggtctcac ccggccagcg ggtcatatgc gccacgtagt agatggccgc
     3361 tccttccgtc cggtgcagcc acagctccag ccagcggtcc tggcgcgccc cctggtgctt
     3421 gcggccattg aacacgagga ggtccttcgc ggtgtccaga agatacagct ctccgctctc
     3481 tcttacgatc ttcctcattc cttccttctc ccggcattgt gccgcccggg acgccacaat
     3541 accagggtat tacatagtaa tacttctaaa taaatactat tacgaagtat tgtttacata
     3601 atagcacggg attacataat aacaaaaaga caatatggag gtggagcatg aacgagagcc
     3661 gtcaggccac gaagaaaata tgcatatcca ttgacaagga gctgctggat tggatcgacg
     3721 cccaggtccg ggagcgccgg ttcgcgacgc gctcccacgg catcgccctc gcggtccagg
     3781 agctgggacg gaaggaacag ggctcggcgt gagaggggcg atcggcccag atcacgaccg
     3841 cgtcaatggg accggcccgg ggaagcccct ggaccactcg gggaaagctc ggactatcac
     3901 ctggcatcgg agcgatcgcc ctctcggggg cgatgagagc ggccaatatg tccaattctt
     3961 ggatgaggcc acgcgcgcgc gaaggcactc ggtaaaagta gggtgaaagt ggggttcaaa
     4021 acgaattttt caagtctata cgggacccgc aaacggcatt caaaatcaat tttatgagcc
     4081 tatacgggat cgcggaacgc attcaaagcg agttttgtca gcctatagcg atcccggcac
     4141 gggtcccgcg gccctcacgc gggggcgagt taactcctt
//
LOCUS       DAOSYN010000106         4123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_17590_length_4123_cov_4.214356, whole genome shotgun sequence.
ACCESSION   DAOSYN010000106 DAOSYN010000000
VERSION     DAOSYN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17590_length_4123_cov_4.214356"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1982)
                     /locus_tag="PLJ11_05180"
     CDS             complement(<1..1982)
                     /locus_tag="PLJ11_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HOO04097.1"
                     /translation="MVRFRKQLRKCRKAVSEIIGNLLILAITVTLFSSIMFYVATIPS
                     PEEQTYAEFDYDLSDLEGNARWVYVTHKGGQELMNGSTNIYIFTYGSTLLSLKMNDSV
                     NPLGDAWSPGEVWKYKLEGINPDTPLSVMIIDTAHNNIVWEAEMVGGQASALYAPIIT
                     SRGTKPATIIHGTAFRFFATVVDPNGDLDPASIYVNATSIGLGLVQLTDEDNDGVFLS
                     APLNGNIAYNDKEVMITAKDLTNRGTSSRFTLSAQQAGGGSQNTTNIGPFYEYESYLV
                     NGTYPPDASGGESGGSLGTTFYYIRRASDNKITRTFQPGEALYIEIYSNKLINLALEN
                     SFSLYNPATGALLTPPTKLINAFSYGGIYATFNRYYINLTAPTQPLYFPLQISLRDNT
                     GTVANIADSISVVGATYPIIKTYKLNTTTNELVLCTSFNHTDTVYLKIFTKDVDLSLT
                     TVTLNDIQINAYSGKYIVKQTLPAPNTWISGSEPVMTSNPPLSQLFKTSKTGSSSSRV
                     LDSKVGDAVYTVKIDLLRPNYGWWLSGKNSYTLFIPVLTDGGSYGTGETYYNLNYQFN
                     VTAPRSTTDLVASVGSGSFTWSASGASWTDNKLVWYKNGERFDQWDATVIDGDTYKGP
                     IGMELADIDNDGYSDLVVGFQDPTVSIAWYKNEKTDG"
     gene            complement(2063..2590)
                     /gene="cobO"
                     /locus_tag="PLJ11_05185"
     CDS             complement(2063..2590)
                     /gene="cobO"
                     /locus_tag="PLJ11_05185"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOO04098.1"
                     /translation="MEGNELGLLHVYTGEGKGKTTAALGLALRAWGHGLRVCVVQFMK
                     RGEDYGEIRALRRMEVDVFQFGSGRLIIKGGHCQEDLDCACRALEFARLVLASGEYDL
                     VVLDEVNVAIYFGLVDCADVLAALRARTPGVEVICTGRNAPDELLEEADLVTVMTVQK
                     HPYDDGLGARKGIEY"
     gene            2659..3177
                     /gene="cyaB"
                     /locus_tag="PLJ11_05190"
     CDS             2659..3177
                     /gene="cyaB"
                     /locus_tag="PLJ11_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOO04099.1"
                     /translation="MIELEVKAPCRDLVGLEGRLRAMGARRIEELEQVDVYYSHPARN
                     FAASDEALRLRRENARSMITYKGPKLDRDTKMREEVELEVEDLERMALLLERVGFRPV
                     RRVAKRRTVYAVGLVHICLDRVEGLGDFVEMEYTGDDLDAGKGKILELKQALGLEGNE
                     RRSYLELLMEKG"
     gene            complement(3181..3432)
                     /locus_tag="PLJ11_05195"
     CDS             complement(3181..3432)
                     /locus_tag="PLJ11_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04100.1"
                     /translation="MDEDAPGKNVDFVTLSSEKIPFGRNNFIEVARKKAITKEGENEF
                     ISLSRGYYLPDGTERFKKSVTIPDDPKIKDFVIDKIRTI"
     gene            3599..>4123
                     /locus_tag="PLJ11_05200"
     CDS             3599..>4123
                     /locus_tag="PLJ11_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO04101.1"
                     /translation="MEDDAPDRPCPRCSAKLPPDAAFCPQCGLPVGGGEEASKQYALY
                     APPPRPIREKGTLQTARELMRGIGAVMALLVLVLVTVNVGIMLWGMHLVIPEALEEAT
                     SLFVVLPWLVRFLELPGLAFVGYYLLLIVAVLLSYAFMLYKGGARFRKELRFQPTEHS
                     PIYAIFTLFMAVLAL"
BASE COUNT          725 a         1185 c         1303 g          910 t
ORIGIN      
        1 ccatcggtct tctcgttctt gtaccaggct atggagaccg tggggtcctg aaacccgacc
       61 accaggtccg aatacccgtc gttgtcgatg tcggcaagct ccatcccgat cggccccttg
      121 tacgtgtcgc cgtcgatgac cgtggcgtcc cactggtcga acctctcccc gttcttatac
      181 cagacgagct tgttgtccgt ccaactggcc cccgaggcgc tccaggtgaa ggagccggag
      241 ccgacgctgg ccacgaggtc cgtcgtgctg cggggcgcgg tgacgttgaa ctgatagttc
      301 aggttgtaat atgtctcacc ggtgccatag ctcccaccgt cggtcagcac cgggatgaac
      361 agcgtatagg agttcttccc ggacagccac cagccgtaat tgggcctcag caggtctatc
      421 ttgaccgtgt agacggcatc gcccaccttg ctatcgagga ccctggagga actggaccct
      481 gtcttcgagg tcttgaagag ttgggagagg ggcggattgc tggtcatgac cggttctgaa
      541 cctgagatcc atgtgttggg cgcgggcagg gtctgcttca cgatgtactt tcctgagtag
      601 gcattgatct ggatgtcgtt aagcgttaca gtggtcaaac tcaggtccac atcctttgtg
      661 aagatcttca agtacaccgt gtcggtgtgg ttgaaagatg tgcaaagcac tagttcgttg
      721 gtggtggtgt tgagcttgta ggtcttgatg atcgggtacg tggcgccgac cacggagatg
      781 gagtcggcga tgtttgctac cgttccagta ttgtccctga gggagatctg aagcgggaag
      841 tagagaggct gcgtgggcgc ggtcaggttg atgtagtacc ggttgaacgt ggcatatatc
      901 ccgccgtagg agaacgcatt gatcagtttt gtgggcgggg tcagcagcgc accggtggcg
      961 ggattgtaca gggagaagga gttctccaac gccaggttga tcagcttgtt gctatagatc
     1021 tcgatgtaca gcgcttcgcc gggctggaag gtgcgcgtta ttttattgtc cgaggccctc
     1081 cgaatataat agaaggtggt ccccagggag ccgccgctct ccccgccgct ggcatctggg
     1141 gggtatgtgc cgttcacaag atagctttca tattcgtaga acggtcctat gttggtggtg
     1201 ttctgtgaac ctccgccggc ctgctgggcg gagagcgtga acctggagga ggtccccctg
     1261 ttggtcaggt cctttgccgt gatcatgacc tccttgtcgt tgtaggcgat gttgccgttt
     1321 aagggagcgc tcaggaacac accgtcattg tcctcatcgg tgagctgcac taggcccagc
     1381 cctatggatg tggcgttgac gtagatgctg gcgggatcaa ggtccccgtt cgggtccacc
     1441 actgtggcga agaaccggaa ggcggtgccg tggatgatcg tggccggctt ggtcccccgg
     1501 gatgtgatga tgggggcgta cagggctgac gcctgcccgc ccaccatttc cgcctcccag
     1561 actatgttgt tatgggctgt atcgattatc atcacgctca acggcgtgtc agggttgatg
     1621 ccttccagct tgtacttcca tacctccccg gggctccagg catctcctag ggggttgacg
     1681 gagtcgttca tcttcaacga gagcagggtg ctgccgtatg tgaagatgta tatgttcgta
     1741 ctcccgttca ttagttcctg gccgccttta tgggtgacat acacccatcg agcgttgccc
     1801 tccaaatccg acagatcata gtcgaactcc gcataggtct gttcctccgg cgacgggatg
     1861 gttgccacgt agaacatgat actggagaac agggtcaccg tgatggccag gatgagaagg
     1921 ttccctatta tctccgacac cgccttgcga catttcctaa gctgtttccg gaatcggacc
     1981 atgagctttt cctctctaaa gctagcgctc caggacgtta tctatgagat gaatgaatat
     2041 tcaatgtttt gcatagtttg gctcaatatt cgatgccctt cctggcgccg agcccgtcat
     2101 cgtagggatg cttctgcacg gtcatgaccg tcaccaggtc cgcctcctcc agaagctcgt
     2161 ccggggcgtt ccgaccggtg catatcacct cgaccccggg cgtccgggcg cgcagggcgg
     2221 ccaggacgtc cgcgcagtca acaaggccga agtagatggc cacgttgacc tcgtcgagga
     2281 ccaccaggtc gtactccccc gaggccagga cgaggcgggc gaattcgagg gcgcggcagg
     2341 cgcagtccag gtcctcctgg cagtgcccgc cttttattat tagcctgccg gagccgaact
     2401 ggaagacatc gacctccatc ctccgcagcg cccggatctc cccgtagtcc tcccctctct
     2461 tcatgaactg gaccacgcac accctcaggc cgtgccccca ggcccgcagg gccagcccga
     2521 gggcggcggt cgtcttcccc ttcccctcgc cggtatagac gtgcagcagc cccagctcgt
     2581 tcccttccat cgtatcctcg tccgtccata cctcattcgg ctccgggttt ttatatattg
     2641 caatccctag tgagggggat gatcgaactg gaggtcaagg cgccctgccg cgacctcgtc
     2701 gggctcgagg ggcggctgag ggcgatgggc gccaggcgaa tcgaggagct ggagcaggtg
     2761 gacgtctatt attcccaccc cgcccgcaac ttcgcggcct cggacgaggc gctgcggctg
     2821 cggcgggaga acgcccgctc catgatcacg tacaagggtc cgaagcttga tcgggacacc
     2881 aagatgcggg aggaggtcga gctggaggtg gaggacctgg agcgcatggc gcttctgtta
     2941 gagcgggtcg gcttccgccc tgtcaggagg gtggccaagc gccggaccgt ctacgccgtc
     3001 gggctcgtcc atatctgcct cgaccgggtg gaggggctgg gggacttcgt ggagatggaa
     3061 tacacggggg acgacctgga cgctgggaag ggtaagatac tggagttgaa gcaggccctc
     3121 ggactggagg ggaacgagcg ccgctcctac ctggagctgc tcatggaaaa gggatagggc
     3181 tcagatcgtg cggatcttgt cgatcacgaa gtccttgatc ttgggatcgt ccgggatcgt
     3241 cacggacttc ttgaaccgct cggtgccgtc cgggaggtag tagccgcggg acagggagat
     3301 gaactcgttc tccccctcct tggtgatggc cttcttccgc gccacctcga tgaagttgtt
     3361 gcggccgaag ggtatcttct cggagctcag tgtgacaaaa tccacgttct ttcctggcgc
     3421 atcttcgtcc attgcggttg cctccgtttt gtagagatgc aacattcggg aaaattataa
     3481 aggtttgcct tattgatata tcatgctggc cgagccgcgc ccatcctgcg cggggttgat
     3541 gccactgggg ggaaggggag cgacaggcgt aaatagaacg gccgggatgg atggaggcat
     3601 ggaggacgac gccccggacc gcccctgccc ccgatgctcg gccaagctcc cgccggacgc
     3661 cgccttctgc ccgcagtgcg ggcttccggt gggcgggggg gaggaggcct ccaagcagta
     3721 cgccctctac gccccgccgc cccggccaat aagggagaag ggcacccttc agacggcgag
     3781 ggagctgatg cgggggatcg gagcggtgat ggcgctcctc gtgctcgtgc tcgtgaccgt
     3841 caacgtcggg atcatgctct gggggatgca tctggtcatc ccggaagccc tggaggaggc
     3901 gacctcgttg ttcgtggtgc tcccgtggct ggtcaggttc ctcgagctgc cgggcctggc
     3961 cttcgtcggc tattaccttt tgctcatcgt ggccgtgctg ctctcctacg cgttcatgct
     4021 ctacaagggc ggggcgcggt tccggaagga gctacgcttc cagcccacgg agcattcccc
     4081 catctacgcc atcttcaccc tcttcatggc cgtgctggcc ctg
//
LOCUS       DAOSYN010000107         4118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_17610_length_4118_cov_4.154073, whole genome shotgun sequence.
ACCESSION   DAOSYN010000107 DAOSYN010000000
VERSION     DAOSYN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17610_length_4118_cov_4.154073"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PLJ11_05205"
     CDS             <1..1305
                     /locus_tag="PLJ11_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO04102.1"
                     /translation="RMSNLSKIIKKEVKELLTPATLIPVIIIAVIFGSLGGVFGGVEE
                     SMSEPQKIAVVIEDNGELGQVAFAVLNSSAEIVYNGDDVKEALGTLEDAGGSSLFVIP
                     SNFTSNIMDNRTGTIEAVWVMYGAGLMDSLPSEVAASLLTSVDQAISAYMITMGMTEN
                     ASLVLQPTQLVETTIFKDKVMDGISPGAIAGVMSSQSFVMPLIVMMVIIYAGSIVISS
                     MGSEKENKTLETLLTMPISRTSIVFGKLVGAALVGLLMAIIYMFGMGYYFDSLVGSAS
                     IDLGEYGLTLGVGDYVLVGISLFASLVFALALCMILGIFTKNYKAAQTMTLPVTFLAM
                     IPMFVTMFTDFDTLPTVAKVLVFAIPFSHPMIAMRSLMFDNYALVLSGIAYTSVVAII
                     AMLVAVTLFKKDILLTGKVARSEEKGRSPLGSMIRRFVLKRK"
     gene            complement(1544..1708)
                     /locus_tag="PLJ11_05210"
     CDS             complement(1544..1708)
                     /locus_tag="PLJ11_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04103.1"
                     /translation="MIVLSGLLALLEINLTLWSSALPTSVLVVLMLGTLGASIVYLNT
                     ARRFRKAGRS"
     gene            complement(1705..1962)
                     /locus_tag="PLJ11_05215"
     CDS             complement(1705..1962)
                     /locus_tag="PLJ11_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HOO04104.1"
                     /translation="MDDDVKLALAWFISVILIMLGVVIIAFPPTGGIVLMTSLVAGLV
                     IAGIALLSGRGAWMIAGYNTASPEERAAFDERELCRGVGRS"
     gene            complement(2029..3456)
                     /locus_tag="PLJ11_05220"
     CDS             complement(2029..3456)
                     /locus_tag="PLJ11_05220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO04105.1"
                     /translation="MASFQDFGLREEIMKAIERMGFVEPTPVQVETIPLIMKGKDVIA
                     QAQTGTGKTAAFGIPILEALTKGVKPFSLILVPTRELGAQVSDELKKLSHFMDDVRVL
                     PVYGGRSIDDQMDALRKGVDIVVGTPGRIIDHLRRGTLSLSEIEMLVLDEADRMLDMG
                     FIEDIEYILARTKTPRQTLLFSATIPPAVREIASRHMVDPVTVLIGEEQLVLPTTKQI
                     YFNIERKNKIWALCRVLDAYHPKAMVFVQTKAMVDIVTKRLVSYGYRAAALHGDLTQA
                     KREKVLEDFREGKTAVLVATDVAARGLDIEGVTHVINYDIPEDPEIYVHRIGRTGRAG
                     KEGIAITFITSKEVHLLRKINEFGVTEITKEEVPESGQKDVVRRVMDFEDQADMFGMV
                     LFKVVAGDGEPVERGTLLELLHRKLRIPEIAVGNINVLKDRTEFEVHKDSAKKVLMEL
                     KGLRAGDKRLRVELVHRELPPISMQ"
     gene            complement(3500..>4118)
                     /locus_tag="PLJ11_05225"
     CDS             complement(3500..>4118)
                     /locus_tag="PLJ11_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04106.1"
                     /translation="VYTRKVPVIVAFEGWDAAGKGGAIQRLTAELNPRGYEVVPVGAP
                     TEDERAHHYLWRFYRKLPPAGHMRIFDRSWYGRVLVERVEGLCPPAEWRRAYQEINSF
                     ERMLVEEGAVLVKVWMEIDRETQLRRFQERAADPRKQWKITEEDWRNREKWDLYERAV
                     DEMLFRTSTAHAPWTIVESNDKYHSRVKTLETVVRAMEVKGKGSK"
BASE COUNT          735 a         1363 c         1184 g          836 t
ORIGIN      
        1 cggatgagca acctttcaaa gatcatcaag aaggaggtca aggagctgct gacccctgcc
       61 accctgatcc cggtcatcat catcgcggtg atcttcggct ccctgggcgg ggtgttcggg
      121 ggcgtggagg agtccatgag cgagccccag aagatcgccg tggtcataga ggacaacggt
      181 gagcttggcc aggtggcgtt cgcggtgctc aactcgagcg ctgagatcgt ctacaacggg
      241 gacgacgtca aggaggcgct cggcaccctg gaggatgccg gcggttcgtc cctgttcgtg
      301 atccccagca acttcacgtc caatataatg gacaacagga cggggacgat cgaggccgtc
      361 tgggttatgt acggggcggg gctaatggac tccctgccca gcgaagtggc cgcctccctg
      421 ctcaccagtg tggaccaggc catctccgcc tatatgataa ccatggggat gaccgagaac
      481 gcctccctcg tcctgcagcc gacccagctc gtggagacga ccatattcaa ggacaaggtg
      541 atggacggca tctcacctgg ggcgatagcc ggcgttatgt cctcccaatc cttcgtgatg
      601 ccgctcatcg tgatgatggt gatcatctac gcgggcagca tagtcatctc ctctatgggc
      661 tccgagaagg agaacaagac cctggagacc ctgctcacca tgcccatcag ccgcacctcc
      721 atcgtctttg ggaagctcgt cggggcggca ttggtggggc tcctcatggc gatcatctac
      781 atgttcggca tgggctatta cttcgactcg ttggtgggtt ccgcgtcgat cgacctggga
      841 gagtacgggc tgaccctcgg ggtcggcgac tacgtcctgg tcgggatctc gctcttcgcc
      901 tcgctggtgt tcgcgctggc gctgtgtatg atactgggca tattcaccaa gaactacaag
      961 gccgcccaga ccatgaccct gccggtgacc ttcctggcca tgatcccgat gttcgtgacg
     1021 atgttcaccg acttcgacac gctgccgacg gtggccaagg tgcttgtgtt cgccatcccg
     1081 ttctcccacc caatgatagc catgcgctcc ctgatgttcg acaactatgc gctggtgctc
     1141 tcgggcatag cctacacctc ggtcgtggcc atcatcgcaa tgctcgtggc ggtgacgctg
     1201 ttcaagaagg acatcctgct cacgggcaag gtggccagat cggaggagaa gggcaggtcc
     1261 cccctgggga gcatgatacg gaggttcgtc ctcaagcgca agtgagccgg atgccgtcaa
     1321 gcgttccaaa ccgtttcctt cgttcctcct ttcatcgaac cgcagcgacg gggacgtccg
     1381 ttcctgaggg gtcgcatggc gaattccgat caccaaggtg ggcggtccgc gactcgcggt
     1441 ccatgggata cagggcaatg tccggacgcg ttcccgacgg ccctccgttc gaacctgcga
     1501 tggccgcgat ctcagcaaca gcgatacgcg atgatgtggc cgttcagctc cttcctgctt
     1561 tcctgaaacg ccgcgccgtg ttcagataga cgatcgatgc ccccagcgtc cccagcatca
     1621 ggacgaccag gacggatgtc ggcagggcgg acgaccacaa ggtcagattg atttccagca
     1681 acgccaagag acccgaaagg acgatcatga tcgccccacc cccctacaga gctccctctc
     1741 atcgaacgcc gccctctcct cgggggatgc cgtattgtat ccagctatca tccatgcccc
     1801 ccttccggaa agaagggcga tcccggcgat cacgaggccg gcgaccaggg atgtcatcaa
     1861 cacgatgccg cccgtcggag ggaatgcgat gatgacgaca ccgagcatga tcaggatgac
     1921 cgagatgaac caggccagcg ccagttttac atcgtcatcc aaggttcccc cgcccattca
     1981 taatggtcaa gcccgatgat gggcgcgttc cccgcacatg aaagcgcctc actgcatgga
     2041 gatgggcggg agctcgcggt ggacgagttc gaccctcagc ctcttgtcgc ccgcgcgcag
     2101 ccccttcagc tccatgagga ccttcttggc cgagtccttg tgcacctcga actccgtgcg
     2161 gtccttgagc acgttgatgt tgccaacggc gatctcaggg atgcgaagct tgcgatggag
     2221 cagttccagc agcgtcccgc gctccaccgg ttccccgtcc ccggcgacca ccttgaagag
     2281 caccattccg aacatgtcgg cctggtcctc gaagtccatc accctgcgga ccacgtcctt
     2341 ctgcccgctc tccggcacct cctccttggt tatctccgtg accccgaact cgttgatctt
     2401 cctgagcagg tggacctcct tggaggtgat gaacgtgatc gcgatccctt ccttccccgc
     2461 cctcccggtg cggcctatgc ggtggacgta gatctccggg tcctcgggga tgtcgtagtt
     2521 gatgacgtgc gtcacgccct cgatgtccag gccccgggcg gcgacatcgg tggcaaccag
     2581 caccgcggtc ttcccttcgc ggaagtcctc gagcaccttc tcccgcttgg cctgcgtcag
     2641 gtccccatgc agcgccgccg cgcggtagcc gtaggacacc agcctcttgg tgacgatgtc
     2701 caccatcgcc ttcgtctgca cgaagaccat ggccttgggg tggtaggcgt caaggacacg
     2761 gcacagggcc cagatcttgt tcttgcgctc gatgttgaag tagatctgct tggtggtcgg
     2821 aaggacgagc tgctcctccc ctatgagcac ggtgacgggg tcgaccatgt gccgggaggc
     2881 tatctcccgc accgctggag ggatggtggc cgagaacagg agcgtctgcc tcggggtctt
     2941 ggtcctggcc aagatgtatt cgatgtcctc gatgaacccc atgtccagca tgcggtccgc
     3001 ctcgtcaagg acgagcatct cgatctcact cagggaaagg gtcccccggc gcagatggtc
     3061 gatgatcctc cccggcgtgc cgaccacgat gtccacgccc ttgcggagcg cgtccatctg
     3121 atcgtctatg gacctgcccc cgtacacggg cagcacccgg acgtcatcca tgaaatggga
     3181 aagcttcttg agctcatcgg acacctgcgc ccccagctcc cgcgtcggaa caaggatgag
     3241 ggagaacggc ttcacgccct tcgtgagggc ctccaggatg ggaatgccga aggccgccgt
     3301 cttccccgtc ccggtctggg cctgggcgat gacgtccttc cccttcatga tcagagggat
     3361 cgtctccacc tgcaccgggg tcggctccac aaagcccatc cgttcgatgg ccttcattat
     3421 ttcctcccga aggccgaagt cttggaaact agccatatgc atcactcgta ttgcatgggc
     3481 atagccatgg ggatatttat catttggagc cctttccctt cacctccatg gcgcgcacca
     3541 ccgtctccag ggtcttgacc ctggaatggt acttgtcatt gctctccacg atggtccagg
     3601 gggcgtgggc cgtgctcgtc cggaacagca tctcgtccac cgccctctcg tagaggtccc
     3661 acttctccct gttccgccag tcctcctcgg tgatcttcca ttgcttccgg gggtcggccg
     3721 ccctctcctg gaagcgcctg agctgcgtct ccctgtcgat ctccatccag accttgacca
     3781 gcactgcccc ctcctccacc agcatcctct cgaaggaatt gatctcctgg tacgccctcc
     3841 tccattccgc aggggggcac agtccctcca ccctctccac cagcaccctt ccgtaccacg
     3901 agcggtcgaa gatgcgcatg tgcccggccg gagggagctt ccggtagaac cgccatagat
     3961 aatgatgcgc cctctcgtcc tcggtcggtg ccccaacggg cacgacctcg tatcccctgg
     4021 gattgagctc ggccgtcagc cgctggatcg ccccgccctt cccggccgcg tcccagccct
     4081 cgaaggcaac gattacgggc accttgcgcg tgtacacc
//
LOCUS       DAOSYN010000108         4046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_17974_length_4046_cov_3.660737, whole genome shotgun sequence.
ACCESSION   DAOSYN010000108 DAOSYN010000000
VERSION     DAOSYN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17974_length_4046_cov_3.660737"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            588..3032
                     /gene="gyrA"
                     /locus_tag="PLJ11_05230"
     CDS             588..3032
                     /gene="gyrA"
                     /locus_tag="PLJ11_05230"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO04107.1"
                     /translation="MQVKKVQGAPPAHTYDLQVAGAHEFVANGVIVHNCLGKYHPHGD
                     TAVYDSLVRLAQDFSLRYPLVEGQGNFGSVDGDEAAAMRYTECRLHARAEDMLADLEK
                     DTVDWGDNFDASLKEPLVLPSKLPNLLINGSSGIAVGMATNMPPHNLREVADAIVHLV
                     DNPEADVMDLMQFIKGPDFPTGGSIHGLSGIVEAYQTGRGKLKVRAKAHFEDAENRKR
                     IIVDEIPYQVNKALLIESIAELVKEKRIDGITDLRDESDREGMRIVIDLRKDVMEEIV
                     LNQLYQHTQMEVTFGVINLALVNNQPRVLTLKEELQIFIQHRHEVIVRRTRYDLAQAR
                     KRDHILQGLVKAIGALDETLRIIRSAKSPEEARAGLMARFDLDEEQAKAILDMRLQKL
                     TGMELEAIQLEFQEIERLIRDLEDILANEGRILGIIKAEALEMKERYGDERRTEIVAQ
                     ALDMDIEDLIPNEEMVLMITQDGYIKRMPLDTYKQQRRGGLGLMGMETKEEDVVTDLF
                     VSMTHDSVLFFTNLGKMYSLKAWQVPVGSRQSKGKPIVNLLPKLEEGERIMATRPITK
                     MAGEHYLVFATRAGIVKKTPLAAYANVSSRGLIALGLDEGDKLVDVKLTDGSKEIILA
                     TKKGLAVRFDEAQVRPMGRPARGVIGIRPEEGDEVVSMAIVDGGSALLTVTENGYGKI
                     SMVGKRDAEDERDTYRKTNRGGKGVITIRTEGRNGNVVAVMEVEQDDELILATANGMV
                     QRIRVADIRVMGRATQGVTVMDLREGDKVIAVARLAGRKERQAVQDAEAHPDAGLSPG
                     RMEEGADDPAPEEGKG"
     gene            3137..3835
                     /gene="gvpD"
                     /locus_tag="PLJ11_05235"
     CDS             3137..3835
                     /gene="gvpD"
                     /locus_tag="PLJ11_05235"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD"
                     /protein_id="HOO04108.1"
                     /translation="MRDSIPGLERVFRTDIEAPKVILVTGPPGSMKTSFCYSVISSYL
                     ERSGEFGLYITLEESAESHLRNMRSLNIALSENMEISDFTDLRELDEVVEVDSPTDYM
                     DFIVQMISYHKKKHGDQFTVFALDSLGALYSLMEDVKGVRKKMFYFFKTLRDYNLTSF
                     IVMERGPNTPSELLGDEGFLVDGIIELGLDRTRGKLVRYMRVEKMRACEHSMERHTLE
                     VGEKGLTVLGPTLA"
     gene            3852..>4046
                     /locus_tag="PLJ11_05240"
     CDS             3852..>4046
                     /locus_tag="PLJ11_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04109.1"
                     /translation="MSDEEIPIWQVIDEFKEKIKSQEADIKRRREDLERWEATLRAKE
                     DHLDQVQQAQAAKESELKERE"
BASE COUNT          932 a         1122 c         1329 g          663 t
ORIGIN      
        1 gtttgatgga cggcggcggt tcggttcata aaagccgcaa cgtcgtaaat tatagcacca
       61 tgtccgaggc cctggccaac gatgtgcagt cattgatgca acagctaggc gtccttagtc
      121 agaggtatat tcaggataaa acagatagcg tcgggctggt cagaggagat gcccttcaga
      181 gaaatggacc atcaagggcg attgaagtaa caggccgttc tgtaaagact ttatctaaac
      241 atattaattt atataatgaa tgcaaaaaag aaagaattga gaggatcggg ggctcccaaa
      301 taaaaacctc agcgtttgat gggattccat atgcatccga ggcggtgctc aacaattcat
      361 ccgagcacca tctgggcgga ggttggttcg aggatgatta cggtaatcaa ttccgttctg
      421 gaacatccta cccaaacgga aacaatatcc ttttcagttc acaattaagg gaaaactcat
      481 tgggccgaac gcaaataacg gatcggggtc cgagatcaaa actatgcatg atcgggtctg
      541 atccggctcc aatcattgac gagatcatca acaacaatat ttatttcatg caagttaaaa
      601 aggttcaggg tgcgcccccc gcgcacacct atgacctgca ggtggccggc gctcatgagt
      661 tcgtggcaaa cggcgtgata gttcacaatt gtttaggaaa atatcatcct cacggcgata
      721 ctgccgtcta cgattccctg gtgcgcctcg cccaggattt ctccttaagg tacccgttgg
      781 tcgaaggcca gggcaacttc ggttccgtgg acggggacga ggcggcggcg atgaggtaca
      841 ccgaatgccg cctgcatgcc cgggcggagg acatgctcgc cgacctggag aaggacacgg
      901 tggactgggg ggacaacttc gacgcctccc tgaaggagcc gctcgtgctt ccgtccaagc
      961 tgcccaacct tctgatcaac ggctcatcgg ggatagccgt gggcatggcc accaacatgc
     1021 ccccccacaa cctccgggag gtggccgacg ccatcgtgca cctcgtggac aatcccgagg
     1081 ccgacgtcat ggacctcatg cagttcatca agggccccga cttcccgacc gggggcagca
     1141 tccacggcct gagcgggata gtggaggcct accagaccgg ccggggcaag ctaaaggtca
     1201 gggcgaaggc ccacttcgag gatgccgaga accgcaaacg gatcatcgtg gatgagatcc
     1261 cctatcaggt caacaaggcc ctgctcatcg aaagcatcgc ggagctcgtc aaggagaagc
     1321 ggatcgacgg catcaccgat ctgagggacg agtcggaccg cgaggggatg cgcatcgtca
     1381 tcgacctgcg caaggacgtc atggaggaga tcgtcctcaa ccaactttac cagcataccc
     1441 agatggaggt gaccttcggg gtcatcaacc tggccttggt gaacaaccag ccccgggtcc
     1501 tgaccctcaa ggaggagctc caaatattca tccagcatcg gcacgaggtc atcgtccggc
     1561 gcaccaggta cgacctggcc caggcaagga agcgggacca catcctgcag ggcctcgtga
     1621 aggcgatcgg cgcccttgac gagacgctgc gcatcatccg ctccgccaag tcccccgagg
     1681 aggccagggc cgggctgatg gcccgcttcg acctggacga ggagcaggcc aaggcgatcc
     1741 tggacatgcg cctgcagaag ctaacgggga tggagctcga ggccatccag ctcgagttcc
     1801 aggagataga gaggctgatc cgcgacctgg aggacatact ggccaacgaa ggacgcatcc
     1861 tgggcatcat caaggccgag gcccttgaga tgaaggagag gtacggggac gagcggcgga
     1921 cggagatcgt cgcccaggcc ctggacatgg acatcgagga cctgatcccc aacgaggaga
     1981 tggtgctcat gatcacccag gacgggtaca taaagcgcat gccgctggac acgtacaagc
     2041 agcagcggcg cggtggactg gggttgatgg gcatggagac caaggaggag gacgtggtga
     2101 cggacctctt cgtcagcatg acccatgaca gcgtgctctt cttcaccaac ctagggaaga
     2161 tgtactccct gaaggcatgg caggtgcctg tgggctcccg gcagtccaag gggaagccta
     2221 tagtgaacct ccttcctaaa ctggaggagg gggagaggat aatggccacc cggcccataa
     2281 ccaagatggc cggcgagcat tacctggtct tcgccactcg cgccggcatc gtcaagaaga
     2341 cgccgctggc cgcctatgcc aacgtcagct ccagggggct catcgccctg ggcttggacg
     2401 agggggacaa gctggtggac gtcaagctga cggacggaag caaggagatc atcctggcca
     2461 cgaagaaggg cctggccgtc aggttcgacg aggcccaggt gcggcccatg ggccgccccg
     2521 cccgcggggt catcgggatc aggccggagg agggggacga ggtggtgagc atggccatag
     2581 tggacggggg ctccgccctg ctgacggtca ccgagaacgg ctatggcaag atatccatgg
     2641 tcgggaagag ggacgccgag gacgagaggg acacctaccg taagaccaac cgcgggggga
     2701 agggcgtcat caccatccgc acggagggcc gcaacgggaa cgtggtggcg gtcatggagg
     2761 tggagcagga cgacgagctc atcctggcca ccgccaatgg catggtccag cgcatccggg
     2821 tcgcggacat cagggtgatg gggcgggcca cccagggcgt gaccgtcatg gacctccggg
     2881 agggggacaa ggtcattgcc gtcgctcgat tggcggggcg caaggagcgg caggccgtgc
     2941 aggacgccga ggcgcacccc gacgccgggc tctccccggg tcggatggag gaaggggcgg
     3001 acgatccggc gccggaggag gggaagggct gacaggggcg aacctcttta tcctcaaagc
     3061 gaaataccat ctacggcctg gacaggctcc ggatccggtc atctgggcgt ctcaggcgcg
     3121 gcggtgaggg cgattgttgc gcgattcgat cccagggctg gaaagggtgt tccggaccga
     3181 cattgaggct ccgaaggtca ttctggtcac gggcccgccc gggtccatga agacctcctt
     3241 ctgctactcg gtcatctcga gctatctgga gcggtccggg gagttcgggc tgtacatcac
     3301 gctggaggag tcggcggaga gccatctgcg caacatgcgg agcctcaaca tagcgctgag
     3361 cgagaacatg gagatctccg acttcaccga cctgagggag ctggacgagg tggtggaggt
     3421 ggactccccc accgattata tggacttcat agtccagatg atcagctatc acaagaagaa
     3481 gcacggcgac cagttcaccg tcttcgcgct ggactcgctg ggggcgctgt actccctgat
     3541 ggaggacgtc aagggggtcc ggaagaagat gttctacttc ttcaagaccc tgcgggacta
     3601 caacctcacg agcttcatcg tgatggagag ggggccgaac accccctccg agctgttggg
     3661 cgacgagggc ttcctggtcg acgggatcat cgaactgggg ctcgaccgca cccgcgggaa
     3721 gctggtgcgc tacatgcgcg tggagaagat gagggcctgc gagcacagca tggaaaggca
     3781 caccctggag gtcggggaga aaggcctcac cgtactcggg cccacgctgg cctgacctag
     3841 gaggtgacga gatgtccgac gaggagatcc ctatctggca agtgatcgat gagttcaagg
     3901 agaagataaa gagccaggag gccgacatca aacggcgccg ggaggacctg gaaagatggg
     3961 aagccacgct gagggcgaag gaggaccacc tggaccaggt gcagcaggcc caggcggcca
     4021 aggagtcgga gctcaaggag cgcgag
//
LOCUS       DAOSYN010000109         4045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_17981_length_4045_cov_3.365414, whole genome shotgun sequence.
ACCESSION   DAOSYN010000109 DAOSYN010000000
VERSION     DAOSYN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17981_length_4045_cov_3.365414"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLJ11_05245"
     CDS             <1..630
                     /locus_tag="PLJ11_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOO04110.1"
                     /translation="APLRAYGRDFAGECVARAMDGATVRLGSIDADVRLAMVGGYQCQ
                     NAAVAAEVALEVGRWTEVPRESIIEGLSRARWPGRLDVVRRSPLTVLDVTHTPDGART
                     VAAELDVFPGRPRVLVIGMLRDKDARGTMAHLAPLFDHVVCTNSASPRALSAQGMLAA
                     AAPYARSCQAVEGVGAAIDVAEGIAGRDGFLMVSGSLYTIGEAMQHLEG"
     gene            632..1273
                     /gene="nth"
                     /locus_tag="PLJ11_05250"
     CDS             632..1273
                     /gene="nth"
                     /locus_tag="PLJ11_05250"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOO04111.1"
                     /translation="MDLRWLLRSMQGAVKGTAFPGLIDEEDPWWEKDPFHVLVATVLS
                     QRTRDLNTSRASSELFRRFNTPEAMAAADLAELEALVRPVGFHRAKAKAIKEIARLVV
                     ENGGVPKDIDRLMEWPMVGRKTANCVLAYAFKEPAICVDTHVHRISNRLGLVTCATPE
                     GTEMALRRLFPRALWCEINGTMVRFGQKVCLPRNPKCGLCPVKDVCAFYKRKG"
     gene            complement(1279..1557)
                     /locus_tag="PLJ11_05255"
     CDS             complement(1279..1557)
                     /locus_tag="PLJ11_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04112.1"
                     /translation="MPEERPHDAMESIIEGKKMEAYAEHRTKDMHVCALCGAIGYRKR
                     PMRPVGQKWVCIDCLRALKEMLEGLDQWEAEIQLEKEMAKKIDETMRA"
     gene            1679..2050
                     /locus_tag="PLJ11_05260"
     CDS             1679..2050
                     /locus_tag="PLJ11_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOO04113.1"
                     /translation="MHEVSVMSGIVESVLKELERHDFLRVEEVQLVLGELTFLGEEQL
                     RFAYEVITRGTPLEGSRLVIVPEGTELECLACGYRGKAEMVGEEFHMAMPSLACPRCH
                     GKVRVLKGQSCRVTSLKVVQE"
     gene            2047..3120
                     /gene="hypD"
                     /locus_tag="PLJ11_05265"
     CDS             2047..3120
                     /gene="hypD"
                     /locus_tag="PLJ11_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOO04114.1"
                     /translation="MKDLRYRDEAMAKKILARLREMDLRLRFMHVCGTHQDTLVRFGL
                     ERMLAEAGITIGQGPGCPVCVTTTKEVVEAITLARAGVTITVFGDMMAVPTPIGSLAD
                     IKAEGADVRVVYSVEDALRLSHEVDRMVFMAIGFETTSPSTAYALLNDPPKNFSVLSC
                     HRLLPPALRALFAMGEVRIDGLIQPGHVATITGLDVFSEFAGPNGVPQVVTGFEPLDL
                     LMGVYMLAKQVKEGRSEVENEYRRVVRPEGNPRARELLERAFTPVDRAWRGFPVLPLS
                     ALEVSEDLAEHNARLVYENVLKGAPVVKEDMGGCRCGEVLRGIIRSEECPAFGKGCSP
                     KRPMGPCMVSREGSCNISYRYRE"
     gene            3319..4038
                     /locus_tag="PLJ11_05270"
     CDS             3319..4038
                     /locus_tag="PLJ11_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04115.1"
                     /translation="MNAVEVKGLVKSYGRFQAVRGISFDVREGEVYGLIGPNGAGKTT
                     TLRVLSTLLEITSGSVMVHGIDLRERPDEVRKVISYLPEDAGAYKHLRGREYLRFIAE
                     FFAEGEKADELVARGMEIANLGERIDDKVDTYSKGMARRLLVARALMIRPKLAILDEL
                     TSGLDVLNAREIRRTVKAYAAQGTTMLLSSHNMLEVEMLCDRIALINDGLIVAEGTPA
                     DLKRRYDAQNIEDVFVKVVSG"
BASE COUNT          734 a         1201 c         1421 g          689 t
ORIGIN      
        1 gcgcccctga gggcctacgg aagggacttc gccggcgagt gcgtcgcccg tgcaatggac
       61 ggagcgaccg tccgcctcgg ttccatcgac gccgacgtaa ggctggcaat ggtaggaggc
      121 taccaatgcc agaacgccgc ggtggccgcc gaggtggcgt tggaggtcgg cagatggacc
      181 gaggtcccga gggagagcat catcgagggc ctctccaggg cccgatggcc agggcgcctg
      241 gacgtggtgc gccgttctcc cctgaccgtc cttgacgtga cgcacacccc ggatggggcg
      301 aggacggtgg ccgccgaact cgacgtcttc cccggcaggc cgagggtact ggtcatcggc
      361 atgctcaggg acaaggacgc ccgggggacg atggcccacc ttgcgcccct gttcgaccac
      421 gtggtgtgca ccaattccgc ctccccgagg gcgctgagcg cccaggggat gttggctgcc
      481 gccgccccct acgcgcgaag ctgccaggca gtggagggcg tgggggcggc catcgacgtc
      541 gcggagggga tcgccggcag ggacggattc cttatggtca gcggctcgct ctatactatc
      601 ggcgaagcga tgcagcactt ggagggctga catggacctt agatggctgc tgcgctccat
      661 gcagggcgcg gtgaagggga cggccttccc gggcctgatc gacgaggagg acccctggtg
      721 ggagaaggac cccttccacg tgctggtggc caccgtgctc tcgcaacgga cccgggacct
      781 gaacacctcc cgggcctcct cggagctgtt caggcggttc aacaccccgg aggcgatggc
      841 cgccgcggac ctcgccgagc tggaggcctt ggtgcgtccg gtcgggttcc atcgggctaa
      901 ggccaaggct atcaaggaga tcgcacgtct ggtggtggag aacggagggg tccctaagga
      961 catagacagg ctgatggaat ggcccatggt cggccgaaag accgccaact gcgtcctggc
     1021 ctacgcgttc aaggagccgg cgatatgcgt ggacacgcac gtgcaccgca tcagcaaccg
     1081 gcttgggctg gtcacctgcg ccacgccgga agggacggag atggccctca ggaggctctt
     1141 cccccgggcc ctctggtgcg agatcaacgg gaccatggtc cgcttcgggc agaaggtgtg
     1201 cctgcccagg aatcccaagt gcggactgtg tcccgttaag gacgtctgcg cgttctataa
     1261 aaggaaggga tgaggggctc aggcgcgcat ggtctcatcg atcttcttgg ccatctcctt
     1321 ctccagctgg atctccgcct cccactggtc cagcccctca agcatctcct tcagggccct
     1381 aaggcagtcg atgcagaccc acttttggcc gacgggccgc atggggcgct tcctgtagcc
     1441 gatggccccg cacagggcgc agacgtgcat gtccttggtg cggtgctccg cgtaggcctc
     1501 catcttcttg ccctcgatga tggactccat ggcatcatgc ggtctttcct ctggcatggg
     1561 aaacggcttt cgtattcccc tggggctata tctaggttgc ttcgggccag cggtccttcg
     1621 ttcgccccgc ccttgccggg ggatccaatt aataatggtt cagggattgg cgtaaaggat
     1681 gcatgaggtc tcggtgatgt cgggcatcgt ggagagcgtg ctcaaggagc tggaaaggca
     1741 cgattttctc cgcgtggagg aggtccagct cgtcctgggg gagctgacct tcctgggcga
     1801 ggagcagctg cggttcgcct acgaggtgat caccagggga acgccccttg agggctccag
     1861 gctcgtgata gtgccagagg ggaccgagct agagtgcctg gcatgcgggt accgggggaa
     1921 ggcggaaatg gtgggggaag agttccacat ggccatgccc tcgttggcat gcccgagatg
     1981 ccatggaaag gtccgggtgc tcaagggaca atcgtgcagg gtcacgtcat tgaaggtggt
     2041 gcaggaatga aggacctgag atatcgggac gaggccatgg ccaaaaagat cctggccagg
     2101 ctgagggaga tggacctgcg gctgcgcttc atgcacgtct gcgggacgca ccaggacacc
     2161 ctggtgaggt tcggcctgga aaggatgctc gccgaggcgg ggatcaccat cgggcagggg
     2221 cctggctgcc cggtctgcgt gaccaccacg aaggaggtcg tggaggccat aactttggcc
     2281 agggccgggg tgaccatcac ggtcttcggg gacatgatgg ccgtcccgac cccgataggc
     2341 tcccttgcgg acatcaaggc cgagggggcg gacgtgcggg tcgtgtattc ggtggaggac
     2401 gccctcaggc tcagccatga ggtggaccgg atggtgttca tggccatcgg cttcgagacg
     2461 accagcccgt ccacggcgta tgcgctgctc aacgaccccc cgaagaactt cagcgtgctg
     2521 agctgccaca ggcttctgcc cccggcgctc agggcgctgt tcgcgatggg cgaggtgcgc
     2581 atcgacggcc tgatccaacc ggggcacgtg gccaccatca ccgggctgga tgtgttctcc
     2641 gagttcgccg gtcccaacgg ggtgcctcag gtcgtcaccg gcttcgagcc cctggacctg
     2701 ctgatgggcg tctacatgct ggccaagcag gtcaaggaag ggcggagcga ggtggagaac
     2761 gagtatcgga gggtggtgcg gccggagggg aaccccaggg ccagggagct gctcgaaagg
     2821 gcgttcacgc ccgtggacag ggcctggcgg ggcttcccgg tactgccgct cagcgccctg
     2881 gaggtctcgg aggatctggc ggagcacaac gcccggctgg tctacgagaa cgtcctgaag
     2941 ggcgcgcctg tcgtcaagga ggatatgggc ggatgccggt gcggggaggt cctgaggggc
     3001 atcatccgct ccgaggaatg ccccgcgttc gggaaggggt gctcccccaa gaggccgatg
     3061 ggcccgtgca tggtcagccg ggaggggagc tgcaacatca gctaccggta ccgggaatga
     3121 gcctgggcga ggcgaccctt cccggcatat ccccggaagg gcaaaaggct ctggaaccac
     3181 gcgtccctcg gccaaggtcc cgccgcttcc ggggcgcacc gctggtcatt cgcataggtc
     3241 ctccgtccga ggctgatttt ttatagaatc atgataaatt atattagggt ccatcacttc
     3301 ccctccctcg ggaaatccat gaacgcggtg gaggtaaagg gcctggtcaa gagctatgga
     3361 cgattccaag cagtgagggg catctcgttc gatgtcaggg agggggaggt ctacggcctc
     3421 atcggcccca acggggccgg gaagaccact acccttcgcg tcctgtccac cctgctggag
     3481 atcaccagcg ggagcgtcat ggtccacggg atagacctta gggaaaggcc cgatgaggtg
     3541 aggaaggtca tcagctacct cccggaggat gctggggcct ataaacacct gagggggcgg
     3601 gagtacctaa ggttcatcgc tgagttcttc gccgagggcg agaaggccga cgaactcgtc
     3661 gcccggggga tggagatcgc caacctcggg gagaggatag acgacaaggt ggacacctac
     3721 agcaagggca tggcccggcg cctgctcgtg gcccgcgccc tgatgataag gccaaagctg
     3781 gccatcctcg acgagctgac ctcgggcctg gacgtcctca acgcccggga gatcaggcgg
     3841 acggtcaagg cctacgcggc ccagggcacc acgatgctac tgtcctccca caacatgctg
     3901 gaggtggaga tgctctgcga ccgtatcgcc ctcatcaacg atggcctgat cgtggcggag
     3961 ggaacgcccg cggacctcaa gcggaggtac gacgcgcaga acatcgagga cgtcttcgta
     4021 aaggtggtga gcggatgagc aacct
//
LOCUS       DAOSYN010000110         4042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_17995_length_4042_cov_3.708553, whole genome shotgun sequence.
ACCESSION   DAOSYN010000110 DAOSYN010000000
VERSION     DAOSYN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17995_length_4042_cov_3.708553"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..214
                     /locus_tag="PLJ11_05275"
     tRNA            join(111..148,179..214)
                     /locus_tag="PLJ11_05275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:145..147,aa:Met,seq:cat)
     gene            315..1469
                     /locus_tag="PLJ11_05280"
     CDS             315..1469
                     /locus_tag="PLJ11_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOO04116.1"
                     /translation="MIIYTGKGGVGKTSVAAATALRCARMGYRTIVISTDSAHSLADS
                     LETPLSGEIRSIGNNLDAIEVDVIHEMETRWNEIGKYLSDFMVSQGLDPISSKEMTVW
                     PGMELMSALFYIEEFQKTGRYDVVVMDTAPTAETLRLLSFPDTADWYFDKMYKIFRNT
                     VRLARATVGKVMSIPMPTNAFLDDLEQLKERMKTVRTILLDPRTTSVRLVLNPERMVI
                     NETKRAYTYLSLYNLTVECIVVNRLLPDGARDMYPKAKYEEQSRYMQMIEESFSPLRM
                     LKAYQASTEVVGREALEVLAEQLFDGTDPAQVYSLEKSMEIVNRDGEDTLALKLPFTS
                     KDQVELYKSNDVLIVTVGWYKRSVTLPYSLVKKEAHKAEFKNGRLVISFK"
     gene            1479..1793
                     /locus_tag="PLJ11_05285"
     CDS             1479..1793
                     /locus_tag="PLJ11_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04117.1"
                     /translation="MVGDTDKPEGEQQERQQERPTHPREERALPEDTVVHLIKAGTEF
                     LAAMDTLMPHSRMPPDAQEHLNNIHKETLLLFRSFVDARLKEINRKKEPPAPTLRKIE
                     VD"
     gene            complement(1795..2349)
                     /locus_tag="PLJ11_05290"
     CDS             complement(1795..2349)
                     /locus_tag="PLJ11_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO04118.1"
                     /translation="MQAKEVREILVREVMSSRPRTGRPDMTVKEAAALMLREGVGSLV
                     VLEEGEPVGILTERDILYKVVALGKMPARVRVAQVMSSPVITISPDSSVAVAAKRMSD
                     LHLRRLPVVEDGKLIGMLTEKDILRLSPSLIELTREWGKIGVGGGRSNGQGSLVGYCE
                     SCEAYSFDLTLENGALLCPDCREG"
     gene            2477..3070
                     /locus_tag="PLJ11_05295"
     CDS             2477..3070
                     /locus_tag="PLJ11_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HOO04119.1"
                     /translation="MKDQEGALAVKLARRAIDAETRNLDIDRFDVPDSFREKSGAFVT
                     LRTFPGDELRGCIGYPEPVFPLAEAILRAAQGACHDPRFPPLRPEELDHVVVEVSILT
                     PPEEVKVEHRLDLPGTIVIGRDGLIIERGMYRGLLLPQVPVEWGWDATTFLEQACLKA
                     GLTPDMWLDRRTRIFTFKAEVFQEESPYGQIVRRRTL"
     gene            3075..>4042
                     /locus_tag="PLJ11_05300"
     CDS             3075..>4042
                     /locus_tag="PLJ11_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO04120.1"
                     /translation="MDVVEGRLFFQGRLQRACVGIEDGLIVAVKKVLRGGEHFDFGDR
                     LVLPGAVDPHVHFREPGLTHKEDFRSGSLSALHGGVTCVLDMPNTVPPVIDRAALDDK
                     REAVRGRSWIDYGLFAGIAPTTDVASLGPCVGYKVFLGSSTQSVLLREERELAKAMDA
                     VGRAGRVVSVHAEEEALLTHRDERSLREHEECRPRQAETAAIERLTAYQERARINVCH
                     VSCADSLAMLKASRMGFEVAPHHMLLDASMDLEAWGKVNPPLRRRADREALFQAFCRG
                     DVPMLATDHAPHAPEEKERGFGEAPSGVPGVETGFSMLLALVKRGFL"
BASE COUNT          821 a         1234 c         1285 g          702 t
ORIGIN      
        1 agccggtctt cgcccccgag gtcccggccg aggaggtcct agagcctcca gtccaggagg
       61 aggcctccgc cgtccaggcg gagaccgaac agtaaatcct tttcccgaat gccggggtgg
      121 ctcagcctgg tggagcgtcg gactcatagg gatttggaac aagaccctct ccagggagat
      181 ccgaaggtca ggggttcgaa ccccctcccc ggcactcccc gtcgctcttc tgatcgaacc
      241 ctcagcgcgc ctcgccgcat gcaatcattc aagtatgtcc gcctatcttg catccctccg
      301 gtgagagaac gaggttgatc atctatactg gcaagggagg cgttgggaag acctcggtgg
      361 ccgcggccac cgccttgcgc tgcgcccgga tgggctatcg cactatagtc atcagcaccg
      421 attccgcgca ttccctggcc gattccctgg aaacccctct gtccggggag ataaggagca
      481 taggcaacaa cctcgacgcc atcgaggtgg acgtcatcca cgagatggag acccgctgga
      541 acgagatcgg gaaatacctt tcggacttca tggtctcgca ggggctggac ccgatatcgt
      601 ccaaggagat gaccgtctgg ccggggatgg agctgatgtc cgcgctgttc tacatcgagg
      661 agttccaaaa gacagggagg tacgacgtgg tggtgatgga caccgccccc accgccgaga
      721 cgctgcgcct gctaagcttc cccgacaccg ccgattggta cttcgacaag atgtataaga
      781 tcttcaggaa cacggtccgc ctggccaggg cgacggtcgg gaaggtgatg agcatcccca
      841 tgcccaccaa cgccttcctg gacgatctgg agcagctcaa ggagcggatg aagaccgtac
      901 gcactatcct gttggaccct cgaacgacct cggtccggct tgtgctgaac ccggagagga
      961 tggtgatcaa cgagaccaag cgggcctata cctacctatc cctttacaat ctgacggtgg
     1021 aatgcatcgt cgtcaatcgg ttgctcccgg acggcgccag ggacatgtac ccaaaggcga
     1081 agtacgagga acagtcccgc tacatgcaaa tgatagagga gtccttctcg cccctgagga
     1141 tgctcaaggc ctaccaggcc agcacggagg tcgtcgggag ggaggcgttg gaggtgctcg
     1201 ctgagcagct tttcgacggc accgaccccg cccaagtata ttccctggag aagagcatgg
     1261 agatcgtcaa cagggacggc gaggacacgc tcgccctgaa gctgccattc accagcaagg
     1321 accaggtaga gttatataaa tcgaacgatg tcctaatagt gacggtcggt tggtacaagc
     1381 gctccgtcac gctgccctat tccctcgtga agaaggaggc gcacaaggcg gagttcaaga
     1441 atggccggct ggtcatcagc ttcaagtgag ggatgagaat ggttggcgat accgacaagc
     1501 ccgaggggga gcagcaggag aggcagcagg aaaggccgac gcatcccagg gaggaaagag
     1561 cgctgccgga ggacaccgtc gtccatctca ttaaggccgg gaccgagttc ctggccgcaa
     1621 tggacaccct gatgccccac agcaggatgc ccccggatgc ccaggagcat ctgaacaaca
     1681 tccacaagga aacgctcctg ctcttccggt ccttcgtgga tgcaagactg aaggagatca
     1741 ataggaagaa ggagccaccg gcccccacct tgagaaagat agaggtagac tgatctagcc
     1801 ttcccggcaa tccgggcaaa gcagggctcc gttctccagc gtaaggtcga aggagtacgc
     1861 ctcgcagctc tcgcagtacc ctaccaggga gccctggcca ttggacctgc cccctccgac
     1921 ccctatcttc ccccattccc tggtcagctc gatgagcgag ggggagagcc tgagaatgtc
     1981 cttctccgtc agcataccga tcagctttcc atcctcgacg accggcagcc tccgcagatg
     2041 caggtcggac atgcgcttgg cggccaccgc aacactggaa tcaggggata tggtgatgac
     2101 cggcgaggac atgacctgcg ccaccctgac gcgcgccggc atcttcccga gggcgacgac
     2161 cttgtagagg atgtcccttt cggtcaatat gcccacgggc tccccctcct ccaggaccac
     2221 caggctgccc acgccctccc ggagcatcag ggcggctgct tccttcacgg tcatgtccgg
     2281 tcttcccgtg cgaggacggc ttgacatgac ctcccggacc agtatctccc ttacctcctt
     2341 cgcctgcatg atggatatat ctccttgggc agtagatatc tttgccgttg tcaggggcgt
     2401 gaaatggaac ggcggttggg tgtccgccat ccacagcctt taatacgatg tagaacaaca
     2461 tcaccccgga tcgagcatga aggaccagga aggggcattg gcggtcaagc tggcccggag
     2521 ggcgatagat gcggagacca ggaacttgga catagaccgg ttcgacgtcc cggacagctt
     2581 cagagagaag agcggggcgt tcgtgaccct caggaccttc ccgggggatg agttgcgggg
     2641 ttgcatagga taccccgagc cagtcttccc gctggccgag gcgatcctaa gggcggccca
     2701 gggcgcctgc cacgaccccc gtttccctcc gctgcggccc gaggagctcg atcacgtggt
     2761 cgttgaggtc agcatcctga cgcccccgga ggaggtcaag gtcgagcacc gcctcgacct
     2821 tccggggacg atcgtcatcg gaagggacgg gctgatcatt gagagaggaa tgtacagggg
     2881 cctgctcctg ccccaggtcc ctgtggaatg gggatgggac gcgaccacct tcttggagca
     2941 ggcatgcctg aaggccgggc tcaccccgga catgtggctg gaccgccgga cgcggatatt
     3001 caccttcaag gccgaggtgt tccaagagga atcgccatac ggtcaaatag tccggagacg
     3061 taccctgtga tcacatggat gtggtagagg gcaggctgtt cttccaagga aggctgcaga
     3121 gggcctgcgt gggcatcgag gacggcctca tcgtcgccgt gaagaaggtc ctgagaggag
     3181 gcgagcactt cgatttcggg gaccggctgg tgctgcccgg ggcggtcgat ccgcacgtcc
     3241 atttccggga gcctggcctc acccataagg aggacttccg ctcgggatcg ctctccgccc
     3301 tgcacggcgg ggtcacatgc gtattggaca tgcccaacac cgtgccgccg gtcatcgaca
     3361 gggcggccct cgacgataag cgggaggcgg tccgggggcg ctcctggatc gactacgggc
     3421 tgttcgccgg gatcgccccc accaccgacg tcgcctccct cggcccctgc gttggctaca
     3481 aggtcttcct ggggtcgtcc acgcagagcg tgctgctgcg ggaggaaagg gagctagcga
     3541 aggcgatgga cgccgtgggg agggctgggc gcgtggtcag cgtgcatgcc gaggaggagg
     3601 ccctcctgac gcacagggac gaacgctccc tgcgggagca tgaggaatgc cgcccccgcc
     3661 aggcggagac cgctgcaata gagaggctta cggcgtatca ggaacgggca cgcatcaacg
     3721 tatgccatgt gagctgcgcc gactccctgg cgatgctcaa ggcgtcccgc atgggcttcg
     3781 aggtcgcccc tcatcacatg ctgctcgatg ccagcatgga cctggaggcc tgggggaagg
     3841 tgaacccgcc gttgaggcgg agggccgatc gggaggcgct gttccaggcc ttctgccggg
     3901 gcgacgttcc gatgctggcc acagaccacg ccccccatgc gccagaggag aaggaaaggg
     3961 ggttcggaga ggcgccctca ggggttccag gcgtcgagac cggcttcagc atgcttctgg
     4021 cccttgtcaa aaggggcttc ct
//
LOCUS       DAOSYN010000111         4030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_18061_length_4030_cov_4.109937, whole genome shotgun sequence.
ACCESSION   DAOSYN010000111 DAOSYN010000000
VERSION     DAOSYN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18061_length_4030_cov_4.109937"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PLJ11_05305"
     CDS             complement(<1..1014)
                     /locus_tag="PLJ11_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04121.1"
                     /translation="MGEPMRAAVFICRSHGANLMAYRGLMKHPLVGHSEALPSCCSQA
                     GRDQIRLHLFGGKEQALLVLGCRASEFGRYQEVAASAGVSPDRVAVVPYQQRTASAAE
                     FALARALDGREPVIPREARDGRLLLLGEGHSFEAAAEQAAKEGLVTIALSPDGLDAPV
                     RLLGGPGAFTLEAGEVICRFGAALLAFDLQVEVTRETLGEGPSVVLLVGGEECRPALV
                     RAVEGTLAEGRKAYIVSQESPFSGSSEGVYRALQARGATFLRAAEAEVSAEGVRVHDE
                     HLGETVLIEAGEVVTIASHRPMALDRVLGVFGLPPSRRPLGPAPGDSGVPGVFLCGSA
                     LSGH"
     gene            complement(1007..2173)
                     /locus_tag="PLJ11_05310"
     CDS             complement(1007..2173)
                     /locus_tag="PLJ11_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO04122.1"
                     /translation="MGGGPAGLACAAELAGRGIGCTIIERREALGGRAQELACKGSVH
                     CRQCDVCRAHRLRDQVLASEAVTVLTGVELEQVERCNGRYRFALRRPTEAIDGGRCSR
                     CGKCVEACPTGAMAKRGGRIRLDRGLCRSLQGLECERCVSVCPVGAIDLFGEEHLSVS
                     GGAVVVATGAREFPPAADPRLGWGLVPGVISSMQLEAHLEGRSPIALGPKGKVAFVLC
                     VGSRTSKAGTQMCSAVCCKYSLRQALALRERAPALGITMFIMDWRGLDGEDALLRELE
                     GSGIQVVRARPAEVVEEEGRPAVRYAGDGTVRCEAFDVVILSIGLIPALDDGTLTELG
                     VPRDELGNLVDQGCGDRGLFLAGSCLGPRDIERCIEDGISAARDAIAYLEGGDG"
     gene            2351..3175
                     /locus_tag="PLJ11_05315"
     CDS             2351..3175
                     /locus_tag="PLJ11_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO04123.1"
                     /translation="MRAVAFTVDVDRDVNLACQGDVCSISKGGGAPRFGSSARGLELV
                     LQVLEEMGVKGTFFWEGRTAEVLSARMDLAGMMRGHEVALHGYDHEDFSGKESGLPLD
                     RQGTKEALDRGEGALERVFGRGPRGFRAPYQRTTEALLDELRERGYLYDSSETAPLVD
                     GAVGPYLLGNGLPEAPVCWARDRRGRRIVSYLWPYHEGKRPMGDYLELIEGFQEGLLV
                     IATHSWHLVESYCAGLRPEEEVRRGTDALRTLLEGAERAGAELVTIAAHLRRAHAL"
     gene            3165..3764
                     /locus_tag="PLJ11_05320"
     CDS             3165..3764
                     /locus_tag="PLJ11_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HOO04124.1"
                     /translation="MPCDPSHRQAMMLAWDALARVPDERLAEASGGELRGRELALTTL
                     GQWTMVDLADRTVTTPDLLGGSWGLIALHHLKGCLAWKDDAEWTSYDQVAEGRPFAAA
                     FQARGIAPLADRFGGSPGDLVRTAEALGGRPMALGDAAAALHAFPRLKVAVVVWRGDD
                     EVSGRAVLLFERGGARTLPAEDLAEAGIALSQALISKAR"
     gene            complement(3748..>4030)
                     /locus_tag="PLJ11_05325"
     CDS             complement(3748..>4030)
                     /locus_tag="PLJ11_05325"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOO04125.1"
                     /translation="ETAAQRHLRTIEEAGLGNMMVCMAKTQLSLTDKPELKGAPTDWE
                     LTVREIFVSQGAGFVVPICGRIMLIPGLPSRPSALGIDIDEDGRITGLY"
BASE COUNT          606 a         1450 c         1387 g          587 t
ORIGIN      
        1 atgcccggag agggcggagc cgcagaggaa gacccccggg accccgctgt cccccggagc
       61 gggaccgagg ggccggcggg agggagggag cccgaagaca ccgagcaccc tgtcaagggc
      121 catcgggcga tgggaggcga tggtcacgac ctcgcccgcc tcgatgagga ccgtctcccc
      181 gaggtgctcg tcgtggaccc tcacgccctc cgcggatacc tcggcctccg ccgccctcag
      241 gaacgtcgcc cctctggcct ggagggcgcg gtacacgccc tcggatgatc cggagaaggg
      301 actctcctgg gaaacgatgt acgccttcct gccctcggct aaagtcccct ccaccgcccg
      361 gacgagcgcc ggcctgcatt cctcgcctcc gaccaggagc accacggaag gcccctcccc
      421 cagtgtctcc ctggtcacct ccacctgcag gtcgaacgcg agcagggcgg ctccgaacct
      481 gcagatgacc tccccggcct ccaacgtaaa ggcccctggc cctccgagca ggcggacggg
      541 tgcatcgagc ccgtcgggcg acagcgcgat ggtgacgagc ccctccttgg cggcctgttc
      601 ggccgccgcc tcgaaggagt gcccctcccc gagcaggagg aggcgcccgt ccctcgcttc
      661 ccttgggatg accggctccc ttccgtccaa cgcccgggcg agggcgaact cggcggcgct
      721 cgccgtccgc tgctggtacg ggacgacggc cacccggtcg ggcgagaccc cggcggacgc
      781 ggcgacctcc tggtacctcc cgaactcgga ggccctgcac cccagcacca ggagcgcctg
      841 ctccttccct ccgaacagat gcagcctgat ctggtcccgg cccgcctggg agcagcagga
      901 ggggagcgcc tccgaatgac cgaccagggg atgcttcatc agcccgcggt aggccatgag
      961 gttcgccccg tgcgagcggc agatgaacac cgccgccctc atcggctcac ccatcgcccc
     1021 cctccagata ggcgatggca tctctcgccg cgctgatccc gtcctcgatg cacctttcta
     1081 tgtcccgcgg accgaggcag ctcccggcga ggaacagccc cctgtcccca cacccctggt
     1141 ccaccaggtt ccccagctcg tcccggggga cgcccagctc ggtcagggtg ccgtcgtcca
     1201 gggcgggtat gagccctatc gagaggatga cgacgtcgaa ggcctcgcag cgcaccgtcc
     1261 cgtccccggc gtagcggacg gcggggcggc cctcctcctc gaccacctcc gcggggcggg
     1321 cccgcaccac ctggatgccc gagccctcga gctccctgag cagggcgtcc tccccgtcca
     1381 gccctcgcca gtccatgatg aacatcgtaa tgccgagcgc gggcgccctc tccctcaggg
     1441 caagcgcctg cctcaaggag tacttgcagc acaccgccga gcacatctgg gttcctgctt
     1501 ttgaggtccg ggagcccacg cacaggacga acgccacctt gcccttgggc ccgagggcga
     1561 tgggcgaacg cccctccaga tgggcctcca gctgcatgga cgagatcaca cctggcacca
     1621 ggccccagcc gagcctcgga tcggctgcgg gggggaactc gcgggccccc gtggccacca
     1681 cgacggcgcc cccgctcacg ctcagatgct cctccccgaa caggtcgatg gcgcccacgg
     1741 gacagacgct cacgcaccgt tcgcactcca gcccctgcag ggagcggcat aggcctctgt
     1801 ccagccggat cctgccccct cgtttggcca tcgccccggt ggggcaggcc tccacgcatt
     1861 tcccgcagcg ggagcacctc ccgccatcga tggcctccgt ggggcggcgg agggcgaagc
     1921 gatagcgccc gttgcacctc tccacctgtt ccagctccac gccggtgagg accgtgaccg
     1981 cctcggaagc cagcacctgg tctcgcaggc ggtgcgcccg gcacacgtcg cactgtctgc
     2041 agtggaccga acccttgcac gccagctcct gcgccctccc cccgagcgcc tcgcgcctct
     2101 cgatgatcgt gcagccgatg cccctgcccg ccagctccgc cgcgcaggcc agtccggcgg
     2161 ggcctccccc caccacgatg accttggcta tcgccctccc ccctcgggat cgacgtctac
     2221 ggcatcgagg ggggaggcgc gcccttgaag gtcgggaacg gcgtccatcg cttgcttgcc
     2281 agcattggcc agatattaaa acctttgcgg ggaccgcacc ccttaagtca tcgccccggg
     2341 atatcccctc gtgcgagcgg tggccttcac ggtggacgtg gaccgggatg tgaacctggc
     2401 ctgccaaggg gatgtctgct ccatatccaa gggcgggggg gcgccccgct tcgggtcctc
     2461 cgcgcggggg ctcgagctgg tcctgcaggt gctggaggag atgggggtga aaggcacctt
     2521 cttctgggag ggcaggacgg cggaggtcct gagcgcgcgc atggacctgg ccgggatgat
     2581 gcgggggcat gaggtggccc tccatgggta cgaccatgag gacttctcgg gcaaggagag
     2641 cgggctcccc ctggaccggc aagggaccaa ggaggctctg gaccgggggg agggggcctt
     2701 ggagcgcgtg ttcggccggg ggccgagggg cttccgggca ccctatcaga ggacgaccga
     2761 ggccctcctg gacgagctgc gggagcgtgg ctatctctac gattcctcgg agaccgcccc
     2821 cctggtcgac ggcgccgtcg gcccatacct tttgggcaat gggcttccgg aggcccctgt
     2881 atgctgggcg agggaccgcc ggggaaggag gatcgtctcc tacctctggc cgtaccacga
     2941 ggggaagcgg ccgatggggg actacctgga gctgatagag gggttccagg aggggctgct
     3001 ggtgatcgcc acgcactcct ggcatctggt ggagagctat tgcgcaggcc tccggccgga
     3061 agaggaggtc aggcggggga ccgacgccct tcgaacgctc ctcgaggggg ccgagcgggc
     3121 gggggcggag ctggtcacca tcgccgcgca cctgcggagg gcgcatgccc tgtgatccct
     3181 cccatcggca ggcaatgatg ctggcctggg acgccctggc ccgggtgccg gacgaaaggc
     3241 tggccgaggc ctcgggaggg gagctgcgag gccgggagct ggcgctgacg accctgggac
     3301 aatggacgat ggtggacctg gccgatcgca ccgtcaccac gcctgacctc ctgggcggga
     3361 gctgggggct catcgccctc caccacctga aaggatgcct cgcctggaag gacgacgcgg
     3421 aatggacctc ctatgaccag gtggcggagg gacgcccctt cgccgcggcc ttccaggcga
     3481 gggggatcgc acccttggcg gaccgtttcg gggggagccc cggggacctg gtccgcaccg
     3541 cagaggcgct ggggggaagg ccgatggcgc tcggggacgc ggcggcggcg ctccatgcct
     3601 tcccccgcct caaggtggcg gtggtcgtat ggcgcgggga cgatgaggtc tctggcaggg
     3661 ccgtcctgct tttcgagagg ggaggcgccc gcacgctccc ggcagaggac cttgccgagg
     3721 cggggatagc cctgtcccag gcgctgatca gtaaagcccg gtgatcctgc cgtcctcgtc
     3781 tatgtcgatc ccgagggcgg aggggcggga cgggaggccg gggatcagca ttatgcgccc
     3841 gcatatcggc accacgaagc ccgcgccctg ggacacgaag atctccctca cggtgagctc
     3901 ccagtccgta ggcgcgccct tcagctcggg cttgtccgtg agggacagct gggtcttggc
     3961 catgcacacc atcatgttcc ccagccccgc ctcctcgatg gtccggaggt gcctctgggc
     4021 cgcggtctcg
//
LOCUS       DAOSYN010000112         4021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_18111_length_4021_cov_4.129097, whole genome shotgun sequence.
ACCESSION   DAOSYN010000112 DAOSYN010000000
VERSION     DAOSYN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18111_length_4021_cov_4.129097"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3332)
                     /locus_tag="PLJ11_05330"
     CDS             complement(<1..3332)
                     /locus_tag="PLJ11_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HOO04126.1"
                     /translation="MSEAERLVSDRAEVWKGRLLDVSNRNPLIRFRRLKGSVLSVVLP
                     DAATLFSELRQGRQYTVVHSPEPLEEAGTGRLMVAAQVPEQPVDKVLYTLRSRARIYR
                     NDHGVNILYVAFGSLLWNDASSGETNESPLFLVPVALERQNSFTPYRLQPLDEDTVLN
                     PSLRKRLELDHRIRLPEIELENGLDLPKAMGMVAAAIPPASGWRVEGSCHLALFHFSK
                     LLMYSDLEQGAGTLSSHPVIRALAGDPAALPPEPSGLPRPEEYDSKLRPEDSFHVLDS
                     DSSQLEAVQAALAGVSFVLQGPPGTGKSQTITNMISELIAHGKRVLFVSQKMAALDVV
                     RGNLERCGLGDMCLELHDPKRSRAEIIKEIATGLEDAGRSGGAAVDAQELVEVRRELD
                     GYVRALHMPRGRLGTSVYRMIGQYAELTGTPDLCFDLPGALEVDEERARRMEGTVLRL
                     SQMASAFREPAHPWAGALVTEWSDRLQAKVQEELSALMAAHGRLCTALPALTIPFGLP
                     EPVSLPEVRRLRQLFKDMAGRPRVRREWLEGDLDLLMTMVRDGLASFQDLLGKEGRLS
                     GMGARDILDLDGRGLRHRFDGEHTGPFRALKGRYRQDMRTLRTASGRRLSFEEARFLV
                     RAVDDYQQALAAWKVRRDALMTALRGEFLGDGQELEALSERLEWTKAFRSKHALLMSE
                     RVIDAVTAEEIPREVGQGAMELELRMDDLDAALSAFAARFTADLRLNGRPLQDAALKD
                     LEEWTERAWACRHRYQEWLDLSSLLLAMRALGLEGLLKELREGSLPPEDFVKGFQRRF
                     YRLWIEEALASDPTLTGFRRDRQMGLIARFDELDRKYVEGSRARVRTRAEANLRREME
                     GPAHSMLLKQEMEIARLSKLKRQRKAIKAMLAECWDLVGLLKPCMLMSPLSVAQFLDR
                     ERTRFDVIIFDEASQICPEDAIGSIVRGRQLIVVGDSKQLPPTSFFALAAEDEDAEPD
                     LESILDECETCGMPRRMLMWHYRSKDESLIAFSNHQFYGGRLNTFPSPLFGREGYGVH
                     HVLVPNGTFDRGRTRTNREEARAVAGMVRDHYALKDGRSLGVIAFSEATMEAIDQALD
                     ELRSGDKNLEAALSSEEGEPFML"
     gene            3447..>4021
                     /locus_tag="PLJ11_05335"
     CDS             3447..>4021
                     /locus_tag="PLJ11_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOO04127.1"
                     /translation="MKEDAYWIRDQVQKHNKWFEESLPMIASENLMSPLAKEMMISDF
                     HDRYAEGLPGKRYYNGNIYVDQVEMRCLELAKRIFKCQFADVRPISGTVANMAALMAL
                     TEPGDTITCTSLDHGAHISTAKFGAVGFRGVNTVTYPFNIHDMVIDVDGAAKMIRETK
                     PKVVQFGLSVFLFPAPIRELKDVAAEVNAPI"
BASE COUNT          720 a         1364 c         1221 g          716 t
ORIGIN      
        1 agcatgaacg gctccccctc ctccgaggac agcgccgcct ccagattctt gtccccactt
       61 ctcagctcgt ccaacgcctg gtcgatggcc tccatcgtcg cctccgagaa ggcgatcacc
      121 ccgaggctcc tcccgtcctt cagggcgtaa tggtcgcgga ccattcccgc gaccgccctc
      181 gcctcctccc tgttggttct ggtccttccc cggtcgaagg tcccgttggg aacgaggaca
      241 tgatggacac cgtagccctc ccgcccgaag agggggctcg ggaaggtgtt cagccttccg
      301 ccatagaact gatgattgga gaatgcgatc agcgactcgt ccttggagcg atagtgccac
      361 atcaacatac gccgaggcat cccacaggtc tcgcactcgt cgaggatgct ctcaaggtcc
      421 ggttcggcgt cctcgtcctc ggccgcgagg gcgaagaacg aggtgggggg cagctgcttg
      481 ctgtccccca ccacgatgag ctgcctcccc ctgacgatcg agcctatggc atcctcgggg
      541 cagatctggg acgcctcatc gaagatgata acgtcaaagc gcgtcctttc cctgtccagg
      601 aactgggcca cggagagcgg gctcatgagc atgcaaggct tgagcagccc aacgaggtcc
      661 cagcactcgg ccagcattgc cttgatggcc ttccgctgcc gcttcagctt ggacaggcgg
      721 gcgatctcca tctcctgctt gaggagcatg gagtgggccg ggccctccat ctccctcctc
      781 aggttggcct ccgcgcgggt ccgcacgcgc gccctgctcc cctccacata cttccggtca
      841 agctcgtcga agcgcgctat cagccccatc tggcggtccc gacggaaacc tgtaagcgta
      901 ggatcggagg ccagggcctc ctcgatccat agcctgtaga accgccgttg gaagcccttc
      961 acaaagtcct cgggaggtag ggagccctcc cgcagctcct tgagcagccc ttcaagcccc
     1021 agggcccgca tggccaaaag gagggaggag aggtccagcc attcctggta cctgtggcgg
     1081 caggcccagg ccctctccgt ccattcctca aggtccttga gcgccgcatc ctggaggggc
     1141 cgtccattga ggcgaaggtc cgcggtgaag cgggccgcga aggcgctcaa ggcggcgtct
     1201 aggtcgtcca tgcgcagctc cagttccatc gccccctgcc cgacctcccg tggtatctcc
     1261 tcggccgtga ccgcgtcgat cacccgctcg ctcatcaaaa gggcgtgctt ggaacggaat
     1321 gccttggtcc attccagtcg ctcggacagc gcctccagct cctggccgtc ccctaagaac
     1381 tctccccgca gggcggtcat aagggcgtcc cggcgcacct tccaggccgc caacgcctgt
     1441 tggtagtcgt ccaccgcacg gaccaggaac cgggcctcct caaagctgag gcgacgcccg
     1501 gaggccgtcc gcagcgtccg catgtcctgt cgatagcgcc ccttcagcgc ccggaacggg
     1561 ccggtgtgct ctccgtcgaa cctatggcgc agaccacgtc cgtccaggtc gaggatgtcc
     1621 cgggccccca tccccgacag cctgccctct ttccccaaga ggtcctggaa ggaggcgagc
     1681 ccatccctta ccatggtcat cagcaggtcg aggtccccct cgagccattc ccggcggacc
     1741 cttggccgcc cggccatgtc tttgaacagc tggcggagcc ggcgcacttc cgggagggac
     1801 accggctcgg gcagaccgaa agggatcgtc aatgccggga gcgccgtgca gagccgcccg
     1861 tgggcggcca tcaatgcgga gagctcctcc tgcaccttgg cctgcagccg gtcgctccac
     1921 tcggtgacca gggcgcccgc ccatggatgg gcgggttccc tgaaggccga agccatctgg
     1981 gataggcgga ggaccgtccc ctccatacgc ctcgcccgtt cttcgtccac ctccagcgcg
     2041 ccaggcagat caaagcatag gtccggcgtc ccggtgagct cagcgtattg gccgatcatc
     2101 ctgtacacgc tggtgccaag cctccctcgg ggcatatgca acgccctgac gtacccatcg
     2161 agttcccggc ggacctcgac gagctcttgg gcgtcgacgg ccgcgccgcc cgatcgtcca
     2221 gcatcttcca ggcccgtcgc tatctccttg atgatctccg ccctgctcct cttggggtca
     2281 tgcagctcca ggcacatgtc gcctagcccg caccgctcga ggttcccccg caccacgtcc
     2341 agggcggcca tcttctgcga gacgaagagc acacgcttcc catgggcgat gagctccgag
     2401 atcatgttgg tgatcgtctg gctctttcca gtgccaggcg ggccttgcag gacgaagctg
     2461 acgccggcca gggctgcctg caccgcctcg agctggctcg agtccgagtc caggacatga
     2521 aaggaatcct ccggcctgag cttggagtcg tactcctccg gacgcgggag gccggaaggc
     2581 tcgggcggaa gggccgcagg gtcccctgcc aatgccctta tgaccggatg cgaggacagc
     2641 gttccggccc cctgctcaag gtccgagtac atgaggagct tggagaaatg gaaaagggcc
     2701 aggtggcagg agccctctac cctccatccg gacgccggcg ggatcgcggc ggccaccatc
     2761 cccatggctt tcggcaggtc gagcccgttc tccagctcga tctccggaag ccggatccgg
     2821 tggtccagct ccagccgctt ccgcaggctc gggttgagga ccgtgtcctc gtccagtggc
     2881 tgcaagcgat agggggtgaa ggagttctgc cgctcaaggg ccacgggcac caggaagagc
     2941 ggggattcgt tggtctctcc agaacttgca tcgttccaaa ggagcgagcc gaacgccacg
     3001 tacaatatgt tcaccccatg gtcattgcgg tagatgcgcg ccctactgcg gagggtgtag
     3061 aggaccttgt ccacgggctg ctcggggacc tgggcggcga ccatgagccg gccggtcccg
     3121 gcctcctcca ggggctcagg ggaatgcacc acggtgtact gccgtccctg cctcagctcg
     3181 ctgaacaggg tggcggcgtc ggggaggaca acgctcagca ccgagccctt taaccggcgg
     3241 aacctgatga gaggattgcg gttggaaacg tccaacaacc tgcccttcca cacctcggcc
     3301 ctatcggaga ccaatcgctc tgcctcgctc attcctctcc ggcaaaggca agggttgcca
     3361 gctaataaat catttgacct gcggtccctc agggcagagg gccaacctat ttaatcccct
     3421 ccttttatga gggtgcaagt gaccttatga aagaggacgc ctactggatc cgggaccagg
     3481 tgcagaagca taacaaatgg ttcgaggagt cgctgccgat gatagcctct gagaacctca
     3541 tgagcccgtt ggccaaggag atgatgatct ccgacttcca cgatcgctat gccgagggac
     3601 tgccagggaa gcgctattac aacggtaaca tctacgtgga ccaggtcgag atgcgatgcc
     3661 ttgagctggc gaagcgcata ttcaaatgtc agttcgccga cgtgcggccg atctccggaa
     3721 cggtggccaa catggccgcc ctgatggcgc tcaccgagcc gggggatacg atcacatgca
     3781 cctccctgga ccacggggcg cacatctcca ccgccaagtt cggggcggtg ggcttccgcg
     3841 gggtgaacac agtgacctac ccgttcaaca tacacgacat ggtcatcgac gtggacgggg
     3901 cggccaagat gatccgagag accaagccca aggtcgtgca gttcggcctc tccgtgttcc
     3961 tgttcccagc tcccataagg gagctgaagg atgtggcggc ggaggtcaac gccccaatat
     4021 g
//
LOCUS       DAOSYN010000113         3984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_18302_length_3984_cov_4.087045, whole genome shotgun sequence.
ACCESSION   DAOSYN010000113 DAOSYN010000000
VERSION     DAOSYN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18302_length_3984_cov_4.087045"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PLJ11_05340"
     CDS             complement(<1..1339)
                     /locus_tag="PLJ11_05340"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO04128.1"
                     /translation="MDLERISKRTAHKALEEGAKEAEAYVVRVRSLTAYIDDDRVKNM
                     EEKLDQGISVRALVNGRMGQSSSTLYTNRDLEECGAKAVALAKLSRPDPFFKRFPGFS
                     QPIGHVDVRQAEVVEATTADLRAKVEGIVKAAGEEVKVPRGLLRVAVTERALANSNGA
                     AFRELGTMAYAHFTSMTKGADPGEGCESYYSSGLEALDPHAMGERLCRKAKDAQSATH
                     FKGRMTGSVILPPGELAEMLLAAVGYSLDGENALRKRSPWAEQVGKEVGNQLVNLQDL
                     PWDPRSALSCRYDDEGVPTRERPLVERGVLKTLLYDSYHAQMGNVAPTGNYLRRDPSD
                     ALNLYRRGGVTLPVNLAWKAGGKSVEDMTSEIEEGVIVERLAAPDVNGVTGGFALEVR
                     SARVVRGGAVEGHVDQCLLVGNFYQALRNVGAVGNDVAVHRNCIVPSVRFDGLE"
     gene            complement(1340..2716)
                     /locus_tag="PLJ11_05345"
     CDS             complement(1340..2716)
                     /locus_tag="PLJ11_05345"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO04129.1"
                     /translation="MMDELTLAVERMRAEGADFCDARYQTLVTNGIAVVDAAVRRLSE
                     DRGRGVCLRARKRGSWGYATTVDISKEAVLEAAAKAAMNAKAGTALGRPIPDTGDARA
                     DLKADVKLHPAKVGMDEKVQAVLDMDRAQRVDRRIVNTSSVLREEVRQNMLVNSYGRQ
                     LSWEEVRTTIMVQAVASEAGRTEFFYDIKGGTTGYEVVKGTDLEAFGREVGKEALNML
                     RAEKAPSGLVTCITDPSISGLLAHEVMGHASEADEIVKRRSFLTDMVGRQVASPLITM
                     VDDGTVPGARGSIPFDDEGTPASRTVIIEDGGYKGYMQSLETAAEMGARPTGNGRAQS
                     YDRRVWVRMTNTFFEKGDQGLDEMLSDVRFGVLTDRMISGMEDPVGGGFEAKALRGFL
                     VENGRVTRPLRSFTLTGNALEILGTADAVGDEVRLDGGSCGKGIEDWVPVSSGGPYCR
                     FKIVLGGD"
     gene            complement(2713..3804)
                     /locus_tag="PLJ11_05350"
     CDS             complement(2713..3804)
                     /locus_tag="PLJ11_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOO04130.1"
                     /translation="MKERVRWIFSNCPKDIDAIVVMNGEEPLLDVTFPYVTGTRAGLF
                     EGCLGVITPDGRSVIITSALEETSARGGKAEVLTYSTAQDRRAIATDLLRGFRRIGIN
                     GPGIVHTNYLEVQRLAPSAELVDVGEGLEAARMIKDREEVGRIRRACRIASRVADEIP
                     GMLSKGMKEYEAAAELDYRMMRMGASGAAFGTNASFWASSAEPHHGAEDIALREGDTA
                     LFDFGCKVEGYCSDITRTFFLGEPEDWQRRMYQVVAEAQRAALGTIRAGANGRDVDAA
                     ARAVIDASEFKGLMVHSTGHGLGLSVHDGGRMAPNHDLPLRPGMVLTVEPGVYVRGKG
                     GVRIEDDVLITEEGYEMLTDADRTMRAIP"
     gene            complement(3851..3967)
                     /locus_tag="PLJ11_05355"
     CDS             complement(3851..3967)
                     /locus_tag="PLJ11_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04131.1"
                     /translation="MCETDNLPFPSNPATSYLAALSAIAAIKRIAGNVWIGI"
BASE COUNT          589 a         1511 c         1151 g          733 t
ORIGIN      
        1 tctccagccc gtcgaaccgt acggaaggga cgatgcaatt gcggtgcacg gcaacgtcgt
       61 tccccaccgc ccccacgttc cttaaggcct ggtagaagtt gcccacgagc aggcattggt
      121 ccacatggcc ctcgacggct cccccgcgca ccacccgggc gctgcgcacc tccagggcga
      181 agcctccggt gaccccgttg acatcggggg ccgccagcct ctccacgatg accccttcct
      241 cgatctccga ggtcatgtcc tccacgctct tgccccccgc cttccatgcc aggttcaccg
      301 gcagggtgac gcccccgcgg cggtagaggt tcagggcatc ggacgggtcc ctccgcaggt
      361 agttgcccgt cggcgccaca ttgcccatct gcgcgtggta gctgtcgtac agcagggtct
      421 tgagcacgcc cctctccacc aggggcctct ccctcgtcgg caccccctcg tcgtcgtagc
      481 ggcaggacag cgcggagcgg gggtcccagg gcaggtcctg cagatttaca agctggttcc
      541 cgacctcctt cccgacctgc tccgcccagg gcgagcgctt gcgcagggcg ttctccccgt
      601 ccagagaata gccgacggcc gccaggagca tctcggccaa ttccccaggc ggcaggatga
      661 cggagcccgt catcctgccc ttgaaatgtg tggcgctctg ggcgtccttg gccttgcggc
      721 agagccgttc gcccatggca tgggggtcga gcgcctccag tcccgatgag tagtatgatt
      781 cgcacccctc tcccgggtct gcgcccttgg tcatggaggt gaagtgggca taggccatcg
      841 tgccgagttc cctgaaggcg gccccgttgc tgttcgccag ggcccgttcg gtcaccgcca
      901 ccctgagcag cccgcgggga accttgacct cctccccggc cgccttgacg atcccctcca
      961 ccttggcgcg caggtccgcg gtggtggctt cgacgacttc ggcctgccgg acgtccacat
     1021 ggcctatggg ctgggagaat cccgggaacc gcttgaagaa cgggtccggc cgggacagct
     1081 tggccaaggc caccgccttg gccccgcact cctccaggtc cctgttggtg tacagggtgg
     1141 aggaggactg tcccatcctt ccgttcacca gcgcccgcac cgagattcct tggtccagct
     1201 tttcctccat gttcttgacc ctgtcgtcat cgatgtaggc ggtgagcgag cggacccgca
     1261 ccacataggc ctcggcctcc ttggccccct cctcaagcgc cttgtgcgct gtcctcttgg
     1321 aaatgcgctc caggtccact caatcccctc ccagcacgat cttgaagcgg caatagggcc
     1381 ctccggacga taccgggacc cagtcctcga tcccctttcc gcaggacccc ccgtccagcc
     1441 tcacctcgtc gcccacggcg tccgctgtcc ccaggatctc cagggcattg ccggtgaggg
     1501 tgaaggagcg cagcggcctg gtgaccctcc cgttctccac gaggaacccg cgcagggcct
     1561 tcgcctcgaa ccccccgccc accgggtcct ccatcccgct gatcatgcgg tccgtgagca
     1621 ccccgaaccg cacgtcggag agcatctcgt ccaggccctg gtcgcccttc tcgaagaagg
     1681 tgttggtcat gcgcacccac acccgccggt catagctctg cgcccggcca ttgcccgtgg
     1741 gccgcgcccc catctccgca gcggtctcca gggactgcat gtacccctta tagcccccgt
     1801 cctctatgat gaccgtgcgc gaggctgggg tgccctcgtc gtcgaacggg atgctccccc
     1861 tcgccccagg cacggtgcca tcgtccacca tcgtgatgag cggagaggcc acctgcctcc
     1921 cgaccatgtc cgtcaggaag gagcgccgct tcactatctc gtccgcctcc gaggcatgcc
     1981 ccatgacctc gtgggccagg aggccggaga tgctcgggtc cgtaatgcac gtcaccagcc
     2041 cggagggggc cttctcggcc ctcagcatat tgagggcctc cttcccgacc tccctgccga
     2101 acgcctccag atcggtcccc ttgaccacct cgtaccccgt cgtccctccc tttatatcgt
     2161 agaagaactc ggtgcggccg gcctccgagg ccaccgcctg caccattatg gtggtgcgta
     2221 cctcctccca gctcagctgg cgcccatagc tgttcaccag catgttctgc ctgacctcct
     2281 ccctaagcac agagctggtg ttgactatcc gccgatcgac ccgctgggcc cggtccatgt
     2341 ccagcaccgc ctggaccttt tcgtccatgc ccaccttggc cgggtgcagc ttgacgtcgg
     2401 ccttgaggtc cgcccgggca tcgcccgtgt ccgggatcgg cctcccaagg gcggtgccgg
     2461 ccttggcgtt catggccgcc ttcgccgccg cctcgaggac cgcctccttg gagatgtcca
     2521 cagtggtggc gtacccccag gacccccttt tcctggcccg gaggcacacg cccctgcccc
     2581 tgtcctccga caggcggcgc accgccgcgt ccaccacggc gatgccattg gtgaccaggg
     2641 tctgatagcg ggcgtcgcag aagtccgcgc cctccgccct catgcgttcc accgccaagg
     2701 tcagctcgtc catcatggga tcgccctcat cgtccggtcc gcgtcggtca gcatctcata
     2761 tccctcctcg gttatcagga catcatcctc tatccgcacc ccgcccttcc cgcggacgta
     2821 cacgcctggc tccaccgtaa ggaccatccc cggcctcagg gggaggtcgt ggttcggcgc
     2881 catcctcccg ccgtcgtgca cggacaggcc aagcccatgc ccggtggagt gcaccatgag
     2941 ccccttgaac tcgctcgcgt ctatgactgc ccgggccgcg gcgtccacgt ccctgccgtt
     3001 cgcccccgcc ctgatcgtcc ccagggccgc ccgctgggcc tccgccacca cctggtacat
     3061 tcgcctctgc cagtcctcag gctccccgag gaagaaggtc cgggtgatgt ccgagcaata
     3121 cccctccacc ttgcatccga agtcgaagag ggcggtgtcg ccctccctca gcgcgatgtc
     3181 ctccgcccca tgatgcggct ccgcggaact ggcccagaag gaggcgttcg tcccgaaggc
     3241 cgccccagag gcccccatcc tcatcatgcg atagtcgagc tcggccgcgg cctcgtactc
     3301 cttcatgccc ttgctcagca tcccagggat ctcgtcggcc accctcgagg cgatgcggca
     3361 ggcccttcgg atgcgcccca cctcctcccg gtccttgatc atccgggcgg cctccagccc
     3421 ttcgccgacg tccaccagct ccgcggaggg ggcaagccgc tggacctcca gatagttagt
     3481 gtgcactatc ccgggcccgt tgatgcctat ccttcggaac cctctcagga ggtcggtggc
     3541 gatcgccctc cggtcctggg cggtactata ggtaagcacc tcggccttcc ctccccgggc
     3601 agaggtctcc tccagggcgg aggtgatgat gacggaccgc ccgtccggcg ttatcacacc
     3661 gaggcatccc tcgaagagcc cggccctggt gccggtgaca taggggaatg tgacgtccag
     3721 aagcggttcc tcgccattca tcaccacgat ggcgtcgatg tccttagggc agttcgagaa
     3781 tatccaccgc acgcgctcct tcatcccgct cacgtgggcc cgaagggggt tggcggtata
     3841 aagggaatcc ctagatccct atccacacgt tgccagctat gcgtttgatg gccgcgatcg
     3901 ccgacagcgc cgccagatag ctggtcgcag ggttggacgg gaagggcagg ttgtccgtct
     3961 cgcacacgat atgcccgaac gcgc
//
LOCUS       DAOSYN010000114         3962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_18438_length_3962_cov_4.633990, whole genome shotgun sequence.
ACCESSION   DAOSYN010000114 DAOSYN010000000
VERSION     DAOSYN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18438_length_3962_cov_4.633990"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1089
                     /locus_tag="PLJ11_05360"
     CDS             91..1089
                     /locus_tag="PLJ11_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOO04132.1"
                     /translation="MRSLYLGSVVEKSGKSMVTLGLARNFKGRVGFFKPFKERLIRVG
                     DRVIDQDAYLMRNVLDLEQPEEVLCPFAYDIFRPTHMDAIKAAYGRAACDCELMLIEG
                     TREVTTGYLNDVSGLAIAEAIGADLVLISTPEPASLDKIAMLYKLSASYKIDVRGVVL
                     NKTDDLSVANFLESKGVKVLGCIPHIPEMTYFTVKEVAEALSANVLVGEGHMDRTVEG
                     VFIGAMTPETALKYMRRHRRKAIITGGDRSDVQLAALSTDTSCLILTGGLYPANQVVS
                     RAYERGIPILVTRYDTLAASEMVEHLIARIEPEDTEKVRLVEKAVQDNVDLEAVFG"
     gene            1125..1406
                     /locus_tag="PLJ11_05365"
     CDS             1125..1406
                     /locus_tag="PLJ11_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOO04133.1"
                     /translation="MVKEVRAAHILVANEDKAKELLKMIKGGADFADLARRHSTCPSG
                     KKGGDLGWFKKGMMVKEFEDAAFSHGKGEIVGPVKTQFGWHIIQVQDQR"
     gene            complement(1409..3829)
                     /locus_tag="PLJ11_05370"
     CDS             complement(1409..3829)
                     /locus_tag="PLJ11_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOO04134.1"
                     /translation="MRLRYMELRNYRKFRDARIEFPDGVVAIIGPNGAGKTTVLEAVT
                     WALFGNEGSVVRDGKEGVISYGASPGDECSVVLEFDLGEDSYRLVRAMKGKGLKVDAS
                     LEVNGRLLATGDTVVTNAIVARLGMDHRAFFASVFARQKDLNALANLRPAERKALVLR
                     MLGVDSLDDVIKEVERDAKEVRGQAEQLSGLLRDAQGRDRLGLLREEERSAREALEAV
                     RESRAKLLAERQAAGGRTGTAKALWEGEEERYRKDTMLERRMTGLRAGLQARRSQLAR
                     LEGEVRELEGLRASLPELERREGEYQQARAEGERHAALRESYLQRRRGQEELEAREKE
                     LRALDEERERTAKALAAMPDVEASLATVEASLREVRELASTVSAQDRLAGAEADRLRL
                     QLAEAMRRAEEVRALGEDSNCPVCLRRMGQQYIELLRRYEEEQRTLEKGIAHQEEVRK
                     GLLEQRARVEQRREALEARRDRLLGQARERASLEERARAIERSYVQVTEGIGELRRRL
                     EAIGEVAYDPAAHEEAKRKAAELEGDHLSLSRAQVKLERLPCALREREEAMATIALEE
                     EELAKAVQERAALAFDAEALASRRREYEEARSAEETLVQAIIRADGEERRLASELDSL
                     RQRAEELEGMRARHTELRAEQEMLNRLSQVMRGFKESIISRIVPTLSETSSDLLSQLT
                     EGKYGGMRLDDNYQMWLYDQGEEHRLERFSGGEVDLANLCLRLAISRMITERSGSQIN
                     LLVLDEIFGSQDQTRKRSILETLGQLQRQFRQILLITHIDDVKDSVGAVLRVQEREDG
                     SSTVVLED"
BASE COUNT          612 a         1440 c         1245 g          665 t
ORIGIN      
        1 ggagctggag atgaaccccg tcatggtggg ggacgagggc atgggagtaa cggcggtgga
       61 cgcgttggtc actatcaagg gggagcagca atgagatctc tttatctagg gtcggtggtg
      121 gagaagtccg ggaagtccat ggtcaccctt ggacttgcga ggaacttcaa gggcagggtg
      181 ggcttcttca agcccttcaa ggaacgcctc atacgggtgg gggaccgggt catcgaccag
      241 gacgcctacc tgatgcgcaa cgtgctggac ctggagcagc cggaggaggt gctgtgcccc
      301 ttcgcctatg atatattcag gccgacgcac atggacgcca tcaaggccgc gtacgggagg
      361 gcggcctgcg actgcgagct gatgctcata gagggcacca gggaggtcac cacaggctac
      421 ctgaacgacg tgtccgggct ggcgatcgcc gaggccatag gcgccgacct ggtgctcatc
      481 tccacgccgg aacccgcatc cctggacaag atcgccatgc tctacaagct gagcgccagc
      541 tacaagatcg acgtcagggg cgtcgtcctg aacaagacgg acgacctgtc agtggcgaac
      601 ttcctggaga gcaagggggt caaggtcctg ggctgcatcc cgcacatccc ggagatgacc
      661 tacttcacgg tcaaggaggt ggccgaggcg ctgtccgcca acgtgctggt gggcgagggg
      721 cacatggaca ggacggtgga gggcgtattc atcggggcca tgaccccgga gaccgccctc
      781 aagtacatgc gccggcaccg gcgcaaggcc atcatcaccg gcggggaccg ctcggacgtc
      841 cagctggccg cgctctccac ggacacctcc tgcctcatac tgaccggcgg cctgtacccg
      901 gccaaccagg tcgtgtcgag ggcgtacgag agaggtatcc ccatcctggt caccaggtac
      961 gacaccctgg ccgcctcgga gatggtggag catctcatcg cccgcatcga gccagaggac
     1021 acggagaagg tccgcctggt ggagaaggcc gtgcaggaca atgtggacct ggaggcggtc
     1081 ttcgggtagg agaggttaat accatcctct gcgatgcgca gggcatggtc aaggaagtaa
     1141 gggccgccca catcctggtg gccaatgagg acaaggccaa ggaacttctc aagatgatca
     1201 agggcggcgc cgacttcgcc gatctggcca ggcggcactc cacctgtccc tcgggcaaga
     1261 agggcgggga cctcggctgg ttcaagaagg gcatgatggt caaggagttc gaggacgccg
     1321 cgttctccca cggcaagggc gagatcgtcg gccccgtcaa gacccagttc ggttggcaca
     1381 tcatccaggt gcaggaccag agataggcct agtcctccag caccaccgtg ctggagccgt
     1441 cctccctctc ctgcaccctg agcaccgcgc ccacgctgtc cttgacatcg tcgatgtgcg
     1501 tgatcagcag gatctgccgg aactgccgct gcagctgccc aagcgtctcc aggatgctgc
     1561 gcttgcgcgt ctggtcctgc gagccgaaga tctcatccag caccagcagg ttgatctggc
     1621 tgccggaacg ctccgttatc atgcgggaga tggccaggcg gagacagagg ttggccaggt
     1681 cgacctcccc gccggagaac ctctccaggc ggtgctcctc cccctggtcg tacagccaca
     1741 tctggtagtt gtcgtccagg cgcatgccgc cgtacttgcc ctcggtcagc tgggagagca
     1801 ggtcggagga cgtctcggag agggtgggga cgatgcggga gatgatgctc tccttgaacc
     1861 cccgcatcac ctgggagagg cggttgagca tctcctgctc cgccctgagc tccgtatgtc
     1921 tcgcccgcat gccctccagc tcctcggccc gttggcggag cgagtccagc tccgaggcca
     1981 gccgccgctc ctccccgtcc gcccggatga tggcctgcac cagcgtctcc tccgcgctcc
     2041 gggcctcctc gtactcccgc cgccgggagg cgagggcctc ggcgtcgaac gcgagggcgg
     2101 cgcgctcctg gaccgccttg gcaagctcct cctcctcgag ggcgatggtc gccatagctt
     2161 cctctctctc ccggagggcg caggggaggc gctcgagctt gacctgggcc ctactcaagg
     2221 aaaggtggtc gccctccagc tcggcggcct tgcgcttcgc ctcctcgtgc gccgcgggat
     2281 cgtacgccac ctccccgatc gcctcgagcc ggcgccggag ctccccgatc ccctcggtca
     2341 cctggacata ggagcgttcg atcgcacgcg cccgctcttc cagcgaggcc ctttcccgcg
     2401 cctgcccgag caggcggtcc cgccgggcct ccagggcctc gcggcgctgc tcgacgcgcg
     2461 ccctctgttc cagcagcccc ttccgtacct cctcctgatg ggcgatgccc ttctccaggg
     2521 tacgctgctc ctcctcgtac cggcggagca gctcgatgta ctgctgcccc atccgccgga
     2581 ggcacacggg gcagttgctg tcctccccca gcgccctgac ctcctccgcc ctgcgcatgg
     2641 cctcggcaag ctggagccgc agccggtccg cctccgcccc ggcaagcctg tcctgggcgg
     2701 agacggtgga ggccagctcg cgcacctccc tcaggctggc ctccacggtg gccaggctgg
     2761 cctccacgtc gggcatggcc gccaacgcct tggcggtccg ctcccgctcc tcgtccagcg
     2821 ccctcagctc cttttctcgc gcttcaagct cctcctgccc gcgccgccgc tgtagatagc
     2881 tctcccggag cgcggcatgc cgctccccct ccgcccgggc ctgttgatat tccccttccc
     2941 tccgctccag ttcggggagg gaggcgcgca gcccctccag ctccctcacc tcgccctcga
     3001 gcctggcgag ctgggagcgc ctggcctgca gccccgcccg gagcccggtc atgcgcctct
     3061 ccagcatggt gtccttccgg taccgctcct cctcgccctc ccagagcgcc ttggccgtcc
     3121 cggtcctccc gccggcggcc tgccgctccg ccagcagctt tgcgcggctt tcccggacag
     3181 cctccagggc ctcccgggcg ctccgctcct cctcgcgcag gaggccgagc ctgtccctgc
     3241 cctgggcgtc gcggagcagc cccgagagct gctccgcctg cccccggacc tccttcgcgt
     3301 cccgctccac ctccttgatg acatcgtcca gggaatcgac ccccagcatg cggagcacca
     3361 gggccttgcg ttccgctggc cgcaggttgg cgagggcgtt caggtccttc tgtcgcgcga
     3421 acacggaggc gaagaaggcc ctgtggtcca tgcccagcct cgccacgatg gcgttggtga
     3481 caacggtgtc ccccgtggcg agcagcctcc cgttgacctc cagggaagcg tccaccttga
     3541 gccccttgcc cttcatggcc cgcaccaggc ggtaggagtc ctccccgagg tcgaactcga
     3601 gcactaccga gcactcgtcc cccggggacg cgccgtagct gatcaccccc tccttgccgt
     3661 ccctcaccac cgacccctcg ttcccgaaca gcgcccaggt gaccgcctcc aggaccgtcg
     3721 tcttccccgc gccgttaggg ccgatgatgg ccaccacccc gtccgggaac tcgattcggg
     3781 cgtcccggaa cttgcggtag ttgcgcagct ccatgtagcg cagcctcatc cctcccccct
     3841 cctcaggtag gccaggccgc ggacgcgcag cgcctccctg tccacgccct ccaccgggac
     3901 ctgcgataga taggactgga attcctgctc cagggagccg agggaggccg agcgccactg
     3961 ca
//
LOCUS       DAOSYN010000115         3955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_18473_length_3955_cov_3.023333, whole genome shotgun sequence.
ACCESSION   DAOSYN010000115 DAOSYN010000000
VERSION     DAOSYN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18473_length_3955_cov_3.023333"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1452)
                     /locus_tag="PLJ11_05375"
     CDS             complement(<1..>1452)
                     /locus_tag="PLJ11_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOO04135.1"
                     /translation="LVECAVLCRAAHHRAIDRVTVPENALDVLAQTLVGMSLERRWTV
                     EEALRLVRGSYCYRGLSEEQLLQTLRYLGSKDAFEGVYSKLWYDPEEGAFGKKKGARM
                     IYFLNLGTIPEEAAYKVFNERGGLLGDLSEKFVERLSVRDVFVLGGRPYEFVRAKGMR
                     VYVRSAPGRKPTVPSWTGEMLPRSFDLSMEIARFRGEMEPRLDAPEGEVVQWLMDDFS
                     IDHGSALSLLHYFKEQRAAAGVPNQRRLLVEGYLDPQGNSGVIFHFPFGRRVNDALSR
                     AYAFLLTQRMGCNISVSISDDAFLLMASKRIDLAALPGLLRSGELRGVLRRAVKDSEI
                     FKQRFRHVAARSFMVLRNYKGREVSVNRQQVRSQYLLDYLAEQESVPVIEETYREVLE
                     DVMDIQRAEQVVQAMEAGEMTVTTVPYSPVPSPFGHSIVLAGISDIVMMEDRSSLLRE
                     LHRKVLSKVLGEGQRFQFEDEAVSAHFRAKRGIA"
     assembly_gap    1453..1462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1463..2679)
                     /locus_tag="PLJ11_05380"
     CDS             complement(<1463..2679)
                     /locus_tag="PLJ11_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO04136.1"
                     /translation="MQRVRKRASKQEVLSLLDPLIAEWFDSRFEGLTEPQAYAIPLIH
                     ERKSVLVSSPTGSGKTLTAFTSIINELFKYAKEGRLEDRIYAVYVSPLKALANDINKN
                     LEEPLGQMHELATRKGYGFPQVRVGVRSGDTSPAERQRQLRRPPHIFITTPESLALVL
                     TAPKFREKFSQVEYVIVDEIHEICDSKRGAHLSLTLERLQALCQRPITRIGLSATLAP
                     IEDIAAYLVGCQDGNVRPVEIIEVLTKRDLDLEVMCPTEDMTALPYEIVNARMYDMLR
                     DMVNAHRTTLVFTNTRSGTEQVVYKLRERGIESIEAHHGSLSKETRLDVEDRLKRGEL
                     RCVVSSTSLELGIDIGSVDLVCQIGSPKSVAKGLQRIGRSGHSVGRTPKGRMIVLDLD
                     DLVAQITLPTTGAT"
     gene            2807..3616
                     /locus_tag="PLJ11_05385"
     CDS             2807..3616
                     /locus_tag="PLJ11_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04137.1"
                     /translation="MRWFSFGEEGARLPWGEWGRAVRDPEDMLASIALGEAAHRRCVR
                     TAGLRAEEGAEGALTAFAHILHHMLDELDEDRVLELRYHLQEGWMRTEPWLWERPEEV
                     LWPMGGDLRAELLALRHGLERAVGPELLRLAWSGMVASGRRMSLGPPALGTAIPLALL
                     DRMLVERIPPFLDGEERGGMAFLRSALRLSERASTEELAAALSAQALVLHKGRVHLDG
                     RLGGGGWYTGREVLEWRRRGLRSCCLLIPFRVMFLASVTGASGPLRVAFPD"
     gene            3651..>3955
                     /locus_tag="PLJ11_05390"
     CDS             3651..>3955
                     /locus_tag="PLJ11_05390"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOO04138.1"
                     /translation="MHRSSVALLALALVLCTCVPGGTEAAGVGGTDIVGRVVDETGAA
                     FPGVEVRAVNAASRTIYSAYSGPDGNYTMPSNFVQGRYDVSAHFTNHSANITYYDVQV
                     "
BASE COUNT          608 a         1304 c         1367 g          666 t
ORIGIN      
        1 ggcgatgccg cgcttggccc ggaagtgcgc ggagaccgcc tcgtcctcga actggaaccg
       61 ctgcccctcc ccgaggacct tggacaggac cttgcggtgc agctcccgca gcagggagga
      121 acggtcctcc atcatcacga tgtcggagat gccggccagc actatgctgt gcccgaaggg
      181 cgacgggacg ggggagtagg ggacggtggt caccgtcatc tcccccgcct ccatggcctg
      241 gacgacctgc tcggccctct ggatgtccat gacgtcctcc agcacctccc ggtaggtctc
      301 ctcgatgacc gggaccgact cctgctcggc caggtagtcg agcaggtatt gggagcgcac
      361 ctgctgccgg ttcacggaca cctcccggcc cttgtagttg cgcaggacca tgaaggaccg
      421 ggcggccacg tgccggaagc gctgcttgaa gatctcggaa tccttgaccg cccggcgcag
      481 gaccccgcgc agctcccccg agcggagcag cccggggagc gcggccaggt cgatgcgctt
      541 cgaggccatc agcaggaagg cgtcatcgga gatggagacg gagatgttgc accccatgcg
      601 ctgcgtcagc aggaaggcgt aggcgcggga cagggcgtcg ttcaccctcc ggccgaaggg
      661 gaagtggaag atcaccccgc tgttcccttg ggggtccagg tacccctcga cgaggagccg
      721 tctctggttc ggcaccccgg cggccgcccg ctgctccttg aagtagtgca gcagggacag
      781 ggcggagccg tggtcgatgg agaagtcgtc catgagccat tgcaccacct ccccctccgg
      841 ggcgtccagc ctcggctcca tttccccgcg gaagcgggcg atctccatgg acaggtcgaa
      901 ggagcggggc agcatctcgc cggtccagga ggggacggtc ggcttcctcc ccggggccga
      961 gcgcacgtag acgcgcatcc ccttggcccg cacgaactcg taggggcggc ccccgaggac
     1021 gaacacgtcc ctgacggaga ggcgctccac gaacttctcc gagaggtccc cgagcagccc
     1081 cccgcgctcg ttgaagacct tgtaggccgc ctcctccggg atggtcccaa ggttcaggaa
     1141 gtagatcatg cgtgccccct tcttcttgcc gaaggcgccc tcctcgggat cgtaccagag
     1201 cttggagtac acgccctcga aggcgtcctt gctgcccagg tacctaagcg tctgaaggag
     1261 ctgctcctcg ctcaggcccc tgtagcagta ggagcccctc accagccgca gggcctcctc
     1321 caccgtccac cgcctctcca gggacatccc gaccagggtc tgcgccagga cgtccaacgc
     1381 gttctcgggg accgttaccc ggtcgatggc gcggtggtgc gccgcgcggc acagcaccgc
     1441 gcattcgacc agnnnnnnnn nngtcgcgcc ggtcgtcggc agtgtgatct gtgccaccag
     1501 gtcgtccagg tcgagcacga tcatgcgccc cttcggggtc cttccgacgg agtgcccgct
     1561 ccggccgatg cgctgcagcc ccttcgccac gctcttcggc gagccgatct ggcacacgag
     1621 gtccaccgag cctatgtcga tgccgagctc gagcgacgtc gaggagacga cgcagcgcag
     1681 ctccccccgc ttcaggcggt cctcgacgtc caggcgcgtc tccttggaga gggagccgtg
     1741 gtgcgcctcg atgctctcga tgcccctctc ccggagcttg tacaccacct gctccgtgcc
     1801 ggaccgggtg ttggtgaaga ccagggtggt ccggtgcgcg ttcaccatgt ccctgagcat
     1861 gtcgtacatg cgggcgttca cgatctcgta cggcagggcg gtcatgtcct ccgtggggca
     1921 catcacctcc aggtccaggt cccgcttggt gagcacctcg atgatctcga ccggccggac
     1981 attcccgtcc tggcagccca ccaagtaggc ggcgatgtcc tcgatcgggg cgagggtggc
     2041 cgacagcccg atgcgggtga tcggccgctg gcagagggcc tgcagccgct cgagcgtgag
     2101 cgagaggtgc gccccccgct tggagtcgca tatctcgtgg atctcgtcca cgatcacgta
     2161 ctccacctgc gagaacttct cgcggaactt gggggcggtg agcaccagcg ccagggactc
     2221 gggggtcgtg atgaagatgt gcggcggcct cctcagctgc ctctgcctct ccgccgggga
     2281 ggtgtcgccg gagcgcaccc ccacgcgcac ctgggggaac ccgtagccct tcctcgtcgc
     2341 cagctcgtgc atctggccca gcggttcctc caggttcttg ttgatgtcgt tggccagggc
     2401 cttgagcggg gacacgtaca cggcgtagat acggtcctcc agccgcccct ccttggcgta
     2461 cttgaagagc tcgttgatga tggaggtgaa ggcggtgagg gtctttccgg agccggtggg
     2521 ggaggacacc aggacgctct tccgctcgtg gatgaggggg atggcgtacg cctgcggctc
     2581 cgtcagccct tcgaaccgcg aatcgaacca ttccgcgatg agcggatcga ggagggagag
     2641 cacctcctgc ttgcttgccc ttttccttac cctctgcatg cgcgcacctt gatggagcgc
     2701 tttctatctg atgcgcctct attattagtt tgccctacga ccatcgaaag ggagggcggg
     2761 agataggttt aagggggtcg aggggcaaca caggaacggt gctgggatga ggtggttcag
     2821 cttcggggag gagggggcca ggctgccctg gggggagtgg gggagggccg tgagggaccc
     2881 agaggacatg cttgcgagca tcgcgctggg ggaggccgcg caccggaggt gcgtgaggac
     2941 cgcagggctc cgcgccgagg agggggccga gggggcgctc accgccttcg cccatatcct
     3001 ccaccacatg ctcgacgagc tggacgagga ccgcgtgctc gagctccgct accacctgca
     3061 ggagggatgg atgcggacgg agccatggct ctgggagcgg ccggaggagg tcctctggcc
     3121 gatgggcggc gacctgcgag cggagctgct ggccctgcgg catggcctgg aaagggcggt
     3181 gggcccggag ctgctccgcc tggcgtggag cggcatggtc gcctccggga ggcgcatgtc
     3241 cctgggcccc ccggccctcg gcacggccat ccccttggcc ctgctggaca ggatgctggt
     3301 cgagagaatc ccgccgttcc tggacgggga ggagcgcggg gggatggcct tcctgcgctc
     3361 cgcgctgcgc ctcagcgaac gggcctcgac ggaggagctc gccgcggcgc tgtccgcgca
     3421 agccctcgtc ctccacaagg gcagggtgca cctggacggg aggctggggg gagggggatg
     3481 gtataccggg agggaagtgc tggagtggag aaggaggggg ctgcgctcct gctgcctcct
     3541 catccccttc cgggtcatgt tcctcgcctc ggtcacaggg gcgagcggcc ccctgcgggt
     3601 ggccttcccc gattaaggtt aaatcctcgg ttcccccatg ccttacccat atgcacaggt
     3661 cgtccgtggc gctgctggcc ttggccctcg tgctctgcac ctgcgttccc gggggaacgg
     3721 aggcggcggg ggtgggaggg accgacatcg tgggcagggt ggtggacgag accggcgccg
     3781 ccttccccgg cgtggaggtg cgcgccgtca acgcggcctc tcgtaccatc tatagtgcgt
     3841 acagtgggcc ggacggcaat tacaccatgc ccagcaattt cgtgcagggg cgctacgacg
     3901 tctccgccca cttcaccaac cactcggcca acatcaccta ttatgacgta caggt
//
LOCUS       DAOSYN010000116         3951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_18503_length_3951_cov_4.080852, whole genome shotgun sequence.
ACCESSION   DAOSYN010000116 DAOSYN010000000
VERSION     DAOSYN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18503_length_3951_cov_4.080852"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLJ11_05395"
     CDS             complement(<1..428)
                     /locus_tag="PLJ11_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04139.1"
                     /translation="MRWRRALSSWWFAGLISLAVGIALLPLLHWLMPFGGLSDEGADH
                     LLFFAHLFFLIAAGVCLLITIVPWHAVDLGGRLDRSALTKAGYEVVERGGSIDVRVAR
                     WSVARIREGRTAFRALPHRTALLVMVLLPAIPGLGVGLFL"
     gene            complement(476..1585)
                     /gene="proB"
                     /locus_tag="PLJ11_05400"
     CDS             complement(476..1585)
                     /gene="proB"
                     /locus_tag="PLJ11_05400"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOO04140.1"
                     /translation="MRDIRATRTVIKIGTNTICDAEGKVDAAYLDDVARQLIELERMG
                     VQSIIVTSGAIGTGSSELGLDGVQKDIPHKQACAAVGQAALMLTYRQVFARYDRSVGQ
                     LLLTYNDFSDRKVYLNLRKTMDVLFELGVIPIVNENDVISTDEIGETFGDNDKLSALV
                     ASKIDADLLILLTDVDGLYDRNPETDKDARLIDTVDEITKDIELMAGGGTKGRSKGGM
                     RSKVNAARIAMGAGCNTVIANGRERDVILRVVKGENVGTLFSAKAMYTNRERWILYAS
                     PRGKINVDPGAEEALRAGGSLLPCGIISVEGRFKKGDVVRIGSFAKGVVNLSPGEIMR
                     LKEEYERERAEGGQRAHNGSVVVDHSNIVFHDRPD"
     gene            complement(1582..2886)
                     /locus_tag="PLJ11_05405"
     CDS             complement(1582..2886)
                     /locus_tag="PLJ11_05405"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOO04141.1"
                     /translation="MASIDMDTVEEAARKAKEASYRLQGLTQAVRNEALQEIAAALQD
                     RADEIVRANEADIDEAERAGLAKALVKRLRYDRSKMRESVASLQALVRQEDPIGKVVS
                     RTELDEGLVLERVTCPIGVIGVIFESRPDALVQISSLCLKSGNAVILKGGSEARRTNE
                     ALTKVIVKAVTDLDERFSSAVQLLSTREEIDRLLALDQYVDLIIPRGSNQLVRFIKEH
                     TKIPVLGHADGICHTYVDEAADLEMAMRVCYDAKVQYPAVCNAMETLLVHEGVAVPFI
                     PAIADRLRHAGVELRGDEGSRALAEMKKATEEDWRTEYNDLVLSVRTVPSLEEAIAHI
                     NRYGSHHTDAIITNDERAARRFMEEVDSSSVMWNCSTRFADGYRYGLGAEVGISTNKT
                     HARGPVGLEGLTIYKYRLMGSGHVVADYVGEDARPFIHRKVL"
     gene            complement(2976..3194)
                     /locus_tag="PLJ11_05410"
     CDS             complement(2976..3194)
                     /locus_tag="PLJ11_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04142.1"
                     /translation="MVSPQVEKLFTALVRAGATSEDKAVSAEKATSLSKLPKAQCMNA
                     IQELKKAGALKEKKKNNAVDYYLTKTTL"
     gene            complement(3389..>3951)
                     /locus_tag="PLJ11_05415"
     CDS             complement(3389..>3951)
                     /locus_tag="PLJ11_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04143.1"
                     /translation="YPRCEKMHPYNLLVTFHGDQRGRAEQEVRERMRDLGVYVERLET
                     CDVDGVFLLQVRGDAKALVVQLKRLCHEDPVDFQYTYHWVPIERWVPTDLPEIREEVA
                     ALGNGIGKDETWKMHLHKRHVPEHYDALISYLTDPLDRGTVDLTDPDKVLAVEMLGKC
                     TGLSLLTKHELLDVNKARVEAGAGKV"
BASE COUNT          669 a         1335 c         1176 g          771 t
ORIGIN      
        1 aaaaggaaca accccacccc cagtccggga atggcgggaa ggaggaccat caccaagagc
       61 gcagtgcgat gggggagcgc ccggaaggcc gtccgcccct cccttatcct ggccacggac
      121 cacctggcga cccggacgtc gatagaacct ccccgctcca ccacctcgta gccggccttg
      181 gtgagcgcgg agcggtccag cctgcccccg aggtccacgg catgccaggg gacgatggtg
      241 atgagcaggc acactccagc cgcgatgagg aagaacaggt gcgcgaagaa caggagatgg
      301 tccgccccct cgtccgaaag cccgccgaag ggcatgagcc aatggagcag cggcaagagg
      361 gcgatgccca cggccaggga gatcaggccg gcgaaccacc aactggaaag ggccctcctc
      421 cacctcatgg aagatatgag ggacggaaag gctggtaaag cttactcctc gccgatcagt
      481 cgggccgatc gtggaagacg atgttggaat ggtcgacgac gaccgagccg ttgtgcgccc
      541 tttgcccgcc ttccgccctt tccctttcgt attcctcctt caaccgcatg atctccccgg
      601 gcgagaggtt gaccacgccc ttggcgaagg acccgatccg gacgacgtca cccttcttga
      661 agcgcccctc gacggagatg atcccgcagg gcaggaggct gccgcccgcc ctcagggcct
      721 cctccgcccc cgggtcgacg ttgatcttgc ccctggggct cgcatacagg atccaccgct
      781 cccggttggt gtacatcgcc ttggcggaga agagcgttcc cacgttctcc cccttcacca
      841 ccctcaggat gacgtccctc tcccgcccgt tggcgatgac cgtgttgcac cccgcgccca
      901 tggcgatgcg ggcggcgttc accttggacc gcatgccgcc cttggagcga cctttggtgc
      961 cccctccggc cataagctcg atgtccttgg tgatctcgtc cacggtgtcg atgaggcggg
     1021 cgtccttgtc cgtctcgggg ttgcggtcat agaggccgtc gacgtccgtg aggaggatga
     1081 gcaggtccgc atcgatcttg ctggcgacca atgcggagag cttgtcgttg tccccgaagg
     1141 tttcccctat ctcatccgtg gagatcacat cgttctcgtt cacgatcggg atcacgccaa
     1201 gctcgaacag gacgtccatc gtcttcctta ggttcaggta gaccttccgg tccgagaagt
     1261 cgttgtaggt gaggaggagc tgccccaccg accggtcgta gcgggcgaag acctggcggt
     1321 aggtgagcat gagggccgcc tgcccgacgg cggcgcaggc ctgcttgtgc gggatgtcct
     1381 tctgcacgcc gtccagcccc aactcgctgc tcccggtgcc gatcgccccg gaggtgacga
     1441 ttatggactg gacgcccatc cgctccagtt cgatcagttg gcgggcgacg tcgtcgagat
     1501 aggcagcatc gaccttcccc tccgcatcac atatggtgtt cgtcccgatc tttatgacgg
     1561 tccgggtcgc cctgatgtcc ctcatagcac cttcctatgg atgaaaggcc gggcgtcctc
     1621 ccccacatag tcggccacga catggccgga gcccatgagg cggtacttgt agatcgtcag
     1681 cccctccaat cccaccggcc cgcgggcgtg ggtcttgttg gtgcttatcc ccacctccgc
     1741 gcccagcccg tagcggtagc catccgcgaa acgggtggag cagttccaca tgacggagga
     1801 ggaatcgacc tcctccatga acctccgcgc cgcccgctcg tcgttcgtga tgatcgcgtc
     1861 agtatggtgc gagccgtacc tgttgatgtg ggcgatcgcc tcctccagcg aggggacggt
     1921 cctcacggag aggacgaggt cgttgtactc cgtcctccaa tcctcctcgg tagccttctt
     1981 catctcggcg agcgcgcgcg agccctcgtc cccgcgaagc tccacgccgg catggcggag
     2041 ccggtcggcg atggccggga tgaagggcac ggccacgccc tcatgcacca ggagggtctc
     2101 catggcattg cacaccgccg gatattgcac cttggcgtcg tagcacaccc tcatcgccat
     2161 ttccaggtcg gccgcctcgt ccacataggt gtggcatatg ccgtcggcat ggcccagcac
     2221 agggatcttg gtgtgctcct tgatgaaccg gacgagctgg ttcgatcccc tcgggatgat
     2281 gaggtccacg tactggtcca gggcaaggag ccggtctatc tcctcccggg tggacagcag
     2341 ctgcacggcc gaggagaacc gctcgtcaag gtcggtcacg gccttgacga tgaccttggt
     2401 caaggcctcg ttcgtgcggc gggcctcgga gccgcccttg agtatcacag cgttcccgga
     2461 cttgaggcat agggaggata tctgcaccag cgcatccggc ctcgactcga agatgacgcc
     2521 gatgacccct attgggcagg tcaccctctc caggaccaat ccctcgtcca gctcggtccg
     2581 cgagacgacc ttaccgatgg ggtcctcctg ccgcacgagc gcctgcagcg atgccacgga
     2641 ctccctcatc ttggaacggt catagcgcag acgcttgacg agggccttcg ccagcccggc
     2701 ccgctccgcc tcatcgatat cggcctcgtt cgcccttacg atctcatcag cgcggtcctg
     2761 cagggcggcg gcgatctcct gcagcgcctc gttcctgacg gcctgagtaa gcccctgcaa
     2821 acggtacgag gcctccttgg ccttcctagc ggcctcctct acggtatcca tgtcgatgga
     2881 cgccatggcg gaggaaacaa tgaagctttc gtcttgggcc cgggagggcc tggagcgatc
     2941 tccccgatcc cctcggccga tcggccccgg tccctttata aagtggtctt ggtcagataa
     3001 taatcgacgg cgttgttctt cttcttctct ttgagggcgc ctgccttctt aagctcctgg
     3061 atggcgttca tgcactgagc cttgggcagc ttggacaggg aggtggcctt ctcggcgctg
     3121 accgccttgt cctcagaggt cgcgccggcc ctgaccaacg ctgtgaacag cttctcgact
     3181 tggggggata ccatgaagag cgggagattt tcttctattt attataggtt gcggtccgat
     3241 cgggggccgt tcatctgcgg gcctccgctc cgtccgggca tcgccctccg gaccgtcagg
     3301 acaaacagtc ctcactcatg accggccgaa cggtcggatg ccgtcggaga acaaagctac
     3361 cgctgtcctc ggcctggccg gcccttcctc acacctttcc tgccccggcc tcgacccgcg
     3421 ccttgttgac gtccaaaagt tcatgcttgg tcaggaggga cagccccgtg catttgccca
     3481 acatctccac cgccaacacc ttgtccgggt cggtcaggtc caccgtgccc cggtccaggg
     3541 gatccgtcag gtagctgatc agggcgtcat agtgctcggg cacatgccgc ttgtgcaggt
     3601 gcatcttcca cgtctcatcc ttcccaatgc cattgcccag ggcggcgacc tcctccctta
     3661 tctccggcag atcggtcggg acccatcgtt ctattggcac ccaatggtag gtgtactgga
     3721 aatcgaccgg gtcctcatgg cagaggcgct tgagctggac gaccaaggcc ttggcgtccc
     3781 ctctcacctg aagcaggaag acgccatcca cgtcgcaggt ctcaagccgc tcaacgtaga
     3841 cccccaggtc cctcatccgt tcccgcacct cttgctcggc gcgccccctc tgatcgccgt
     3901 ggaaggtcac caggaggtta tatgggtgca tcttctcaca cctcggatag g
//
LOCUS       DAOSYN010000117         3950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_18508_length_3950_cov_3.180231, whole genome shotgun sequence.
ACCESSION   DAOSYN010000117 DAOSYN010000000
VERSION     DAOSYN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18508_length_3950_cov_3.180231"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2556
                     /locus_tag="PLJ11_05420"
     CDS             <1..2556
                     /locus_tag="PLJ11_05420"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOO04144.1"
                     /translation="IRAAAVQSGSKTLAEQTRVFLQDLEEAVALRSNTAIIVSQLPEE
                     FDPLDEKETLEKVTKGKSGVEKEEIERRAMRETKIAQGLLMRKAESYNPVQDDVELVS
                     ILRRRIFSKVDKEEAKKVAEAYQEFYGSPGPRGLIPNDALGKNAKERMVDDYPFHPKT
                     ISLIRDKLGQAPKFMQTRGSLLLMTYAVRNILEVKKKPQLIHPSHLDPRDTNIRELLA
                     KRVFDDGRLENAIHTELVGKGEGARAQRIDQVFGTDIGSRITASILLESALVGVKGDA
                     DPLVGATKPEIMYDVLEPGEDETRVEKALESILETSYHIIIRNQKYVFRGEVNLNRPI
                     DERKKDIPSYRITNDIKRRMENHVLKGSTPFRQCLWPISPEDIPDIRQLKLVALAPED
                     DYWLDSMDSKILDELFFNKNQSREPRMNKNSLVIVAPNRDQREETEEKVRHSIAVKEI
                     SEDKEFAKSLSDEQKKWLEKERSKSEALATLRIGMAYNLLFYPEAEGKYGKPKLEGVR
                     LSLGESDIGVDTEDWKIKSSRYLENVIKRLDDKGKWRTDPYGSDFLVEKVFSTSDRQI
                     DSRKFKDLEGAFFEDTKLPFLASKDILRKTVEDGVTDGSFVVHSGGRLFTSDQAGKMV
                     LHDDSEVIRRGTQRWDEMLETYCFRCGKLAGECECVERCPTCNRPVSECTCESLCPVC
                     KQKKDECTCSRSPVSWRIDGALLKNLPDRASDLLKERGVKASGLKVFANRPSDVAFVL
                     RLIGTISGRGVEARPDDVMVNISKYGTEKVDISFRASAPAPLWNMLKASTEGMLAKIT
                     AGEAKGDASVTLNLVFKDPLGPEELKDRMRAFTMTGGDKVKVNAQIIPQDKTG"
     gene            2522..2995
                     /locus_tag="PLJ11_05425"
     CDS             2522..2995
                     /locus_tag="PLJ11_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04145.1"
                     /translation="MPRSFLKIKLDDIMEGKRETSWRFERTRRYGSSGWVLSRPKDDE
                     GSMENAAMIFGKRADFVLPLLIDVMRRQGADAKVIKKKGPLEIPEEEGYRLALAFELL
                     RFTDSSARMESILEAIRSFEAEESYLWYSYLALAKMNGKEGRLAVSMAHLGEAMT"
     gene            3023..>3950
                     /locus_tag="PLJ11_05430"
     CDS             3023..>3950
                     /locus_tag="PLJ11_05430"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOO04146.1"
                     /translation="MMALKEYPRLFLEGEDIPSAALGVECKRENSTGDHPPLNRIHIW
                     WARRPLAASRFAIVGSLVGDDWTHEDILEIIGVPRGKDPVLERERIDNANKRGEKLSS
                     PFGYRRAFLNPVNSRILKKFKESSKSLWGTERPTILDPFSGGGSIPFEGLRLGLDVKS
                     VELNPVACVGIDAVTNIPLHNGGDLNIKLKKIGDAIANKLEVALSQFFPKKQGEKIFA
                     YIWVRSIICPKCGFNAPLTSNWWLDTKNGLAFKPIIIDGIDFPEFKIEKAGTSGFNPD
                     KGSQSGGAGVCVRCGKTISSEYIKEEAQKGKMG"
BASE COUNT         1062 a          843 c         1183 g          862 t
ORIGIN      
        1 atcagggctg cagcagttca gtctggctcg aagaccttgg ctgagcagac ccgggtattc
       61 ctgcaggacc tcgaggaggc ggtcgctctt cggtcgaaca ccgccatcat cgtatcccaa
      121 ttacccgagg agttcgatcc gctggatgag aaggagacct tggagaaggt caccaaaggt
      181 aagagcggtg tcgaaaagga ggagatcgaa cgacgggcca tgcgggagac caagatagcc
      241 caaggtctat tgatgcgcaa ggcagagagc tacaatccgg tccaggacga tgttgagttg
      301 gttagcatcc taagacgccg catattcagt aaggtggaca aggaggaggc gaagaaggtc
      361 gctgaggcat atcaggagtt ctatggctcg cccggtccaa gaggcctgat ccccaatgac
      421 gccttgggca agaacgcgaa agaaaggatg gtggatgatt atccgttcca tcccaagacc
      481 atatcattga tccgtgataa gttggggcag gcaccaaagt tcatgcagac cagggggtcg
      541 ctgcttttga tgacctatgc cgtccggaac atattggagg tgaagaagaa acctcagttg
      601 atccacccct cccatcttga tcctagggac actaacataa gggagctttt ggccaagagg
      661 gtctttgatg atgggcggtt agagaacgcc atacacaccg aacttgtggg taagggagag
      721 ggagcccggg cacagcggat agaccaggtc tttggtacgg acatcgggag ccggataact
      781 gcttccattc ttctagagag cgccttggta ggggtgaaag gggatgctga ccccttggtg
      841 ggggccacca agcctgagat catgtacgac gtgctcgagc ctggagagga tgagacccgg
      901 gtggagaagg cgttggaatc gatcctggag accagctacc acatcatcat caggaatcag
      961 aagtatgtgt tcagaggaga ggtcaatctc aacaggccta tcgatgagcg taaaaaggac
     1021 attcctagct accggatcac caacgacatc aagcggagaa tggagaacca cgtgctcaag
     1081 gggagcacgc ccttccgaca atgtctctgg ccgatttcgc ccgaagatat tccagacata
     1141 cgtcaactga agcttgttgc cctcgcgccc gaggacgatt attggctcga ttcgatggat
     1201 tcaaaaatat tggacgaact gttcttcaat aagaaccagt cgagagagcc tcggatgaat
     1261 aagaactcct tggtgatagt ggcccccaac agggaccaga gggaggagac tgaggagaag
     1321 gtcagacatt cgatagccgt gaaggagata tctgaggaca aggaatttgc caagagccta
     1381 tccgacgagc agaagaaatg gttggagaag gagcgctcga agtccgaggc actggccacg
     1441 ctacgtatcg gtatggccta caaccttttg ttctaccctg aggccgaggg caagtatggg
     1501 aagccaaaat tggaaggtgt gaggctgagc ctgggagagt ccgacatagg agtggacact
     1561 gaggattgga agatcaagag cagccgatat ctggagaatg tgatcaaacg gttggatgat
     1621 aaagggaaat ggcgcacaga cccatacggc tccgatttct tggtggagaa ggtgttcagc
     1681 acctccgacc gccagatcga ctccaggaag ttcaaggacc tggagggcgc gttcttcgag
     1741 gatacgaagc ttccattcct ggccagcaag gacatcctga gaaaaactgt ggaggatggt
     1801 gtgaccgatg gctctttcgt ggtccacagc ggcggaaggc tcttcacctc ggaccaggcg
     1861 gggaagatgg tcctgcatga tgattcggag gtgataaggc gaggaaccca gagatgggat
     1921 gagatgcttg agacgtactg tttcagatgt gggaagttgg cgggggaatg cgagtgcgtg
     1981 gagcgttgtc caacatgcaa ccgcccggtc agcgaatgta cctgtgaatc gttatgtccg
     2041 gtgtgcaagc agaaaaagga cgaatgtacc tgttctcgat ctccagtgag ttggaggatc
     2101 gatggcgccc ttctcaagaa cctacctgac cgtgcctcgg atttgttgaa agagagagga
     2161 gtgaaggcct ccggactgaa ggtgttcgcc aataggccaa gtgacgtagc cttcgtatta
     2221 cgcttgatag gcaccatctc aggtcgaggg gtggaggcaa ggcccgacga tgtaatggtg
     2281 aacatctcga aatatgggac ggagaaggtg gacatatcct tcagagcttc cgcccctgct
     2341 ccgctctgga acatgttgaa ggcttcgacc gaaggtatgc ttgctaaaat tactgcgggg
     2401 gaggccaagg gagatgctag tgtaactcta aacctggtct tcaaggaccc gttaggccct
     2461 gaggagctaa aggataggat gagagccttc accatgaccg gtggcgataa ggtcaaggtg
     2521 aatgcccaga tcattcctca agataaaact ggatgatatc atggaaggta aaagggaaac
     2581 atcgtggaga ttcgagcgca ccaggaggta cggaagcagt ggttgggtgc tctcccgccc
     2641 taaggacgat gaaggcagta tggagaatgc tgccatgata ttcgggaaga gggcggattt
     2701 cgtccttccc ctgctgattg atgtgatgcg gaggcagggt gcggacgcca aggtgataaa
     2761 gaagaaaggg cctctggaaa tccccgaaga ggagggttac cggctcgcct tggcctttga
     2821 gctactacga tttaccgatt cctcggcgag gatggaatcg attctagagg ccataaggtc
     2881 cttcgaggcg gaggagtcct acctttggta ctcctacctc gccctggcga agatgaatgg
     2941 caaggagggc agattggcgg tgtccatggc ccatcttggt gaggcgatga cttaatcgtg
     3001 agtgatttct aaataggggg aaatgatggc attaaaagaa tatcctagat tgttcttgga
     3061 gggtgaggac attccctcag cagctcttgg ggttgaatgt aagagggaga acagcactgg
     3121 tgaccatcca ccccttaata gaatccatat ttggtgggca cgccgacctc tcgctgcatc
     3181 gaggtttgcg atagtgggaa gtttggtagg tgatgattgg acccatgaag acatcctgga
     3241 aattatcgga gtccctaggg gaaaggaccc cgttttagag cgtgagcgga ttgataacgc
     3301 taataaacgt ggagaaaaat tgtcatctcc ctttggttat agaagagcgt tcctcaatcc
     3361 agtgaactca agaattctca agaagtttaa ggagagttcg aaatcgttgt ggggtactga
     3421 aagaccgaca atattggatc cattctccgg aggcggtagt attcccttcg aaggtttacg
     3481 cttaggtttg gatgttaaaa gcgttgagct aaaccccgtt gcatgtgttg ggattgatgc
     3541 tgtcaccaat attccacttc acaacggcgg tgatctaaat ataaaattga aaaaaattgg
     3601 tgacgcgatt gccaataaat tagaggttgc gttatctcag ttctttccaa agaagcaagg
     3661 agagaaaata tttgcttaca tttgggtccg ttcaataata tgcccaaaat gtggttttaa
     3721 cgccccgctg acatctaact ggtggcttga tacgaaaaac ggacttgctt ttaaacccat
     3781 cataatagat ggtatcgatt ttccagaatt caagattgag aaggctggaa ccagtggttt
     3841 taatccagat aagggaagtc aatctggagg ggccggtgtt tgtgtcaggt gcggtaagac
     3901 tatttccagt gaatatatca aagaagaagc acagaaaggt aaaatgggaa
//
LOCUS       DAOSYN010000118         3877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_18930_length_3877_cov_3.513344, whole genome shotgun sequence.
ACCESSION   DAOSYN010000118 DAOSYN010000000
VERSION     DAOSYN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18930_length_3877_cov_3.513344"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2856
                     /locus_tag="PLJ11_05435"
     CDS             <1..2856
                     /locus_tag="PLJ11_05435"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOO04147.1"
                     /translation="MSVVNARSEWLEVRVRREGKEHYLRCEKGVVTVPLRELGPAEGS
                     GTTITFMPDRTIFETVQFEYDALAEKFKDLAYLNRTAVITFRDEASGRQDRFHFEGGI
                     TEFVEDLNHNKTKLHDKPIYIYAEKDNVIVELAMQYTDSYSENIYSFVNNINTAEGGT
                     HMVGLRSALTRAMNDYAKKNNMLKGGLDALSGEDVREGLTAILSIKIPEPQFEGQTKT
                     KLGNSEVKGIVDSLVFEKLTEFLMENPKVAAVCIERAVLAAQAREAARKARDLTRRKG
                     VLESAALPGKLADCSERDPARSELYIVEGNSAGGCFSGDTKVALVDGRELSLEELVKE
                     QAEGKEHFCYTIRKNGTIGVEKAINARRTKVDAPVIRVTLDNGEVITCTPDHQFMIRD
                     GGYLEARSLYPGVSLMPLYRKSADRSQKNIAIDGHEMVWDPNSESWLFTHMLADRYNR
                     WVGTYSVQDGSHCHPIDFNKRNNNPTKLVRMDVDDHLAHHRSHVEKTLHRKDTMEKCR
                     VLKQQPGFEARMSDRMRAGSTAAILSRNAKKQWANPGYKNHMISRWIKFYGSNPDHQL
                     ANRELLMAEAKKYWITPGHRKEQSIRKTILYSNNPGLRENLSAIAQEQWADPKLREWR
                     SQTTKAQWTEEFRKKRKAALYANLYETSYRKTISVLTQYLDTNGHVDVGRYEEHRISI
                     GDESLLKFDTFCERYYQNDVVAADEAIAAFNHKVVMVEDIGERMDVYDIEVPHSHNFA
                     LASGVFVHNSAKMGRNREFQAILPLRGKILNVEKARMDRILKNQEIRNLITAIGANIG
                     PDCDASKARYHRIIMMTDADVDGAHIRTLLLTLFYRYMRPLIDAGFIYIAQPPLYKVS
                     KGGKEVYVYDDEALERTLQEMGRNVNLQRYKGLGEMNPKQLWETTMDPETRTLKRVTV
                     EDAMRADELFTVLMGDEVEPRREFIMSHADEVENLDI"
     gene            2867..>3877
                     /locus_tag="PLJ11_05440"
     CDS             2867..>3877
                     /locus_tag="PLJ11_05440"
                     /inference="COORDINATES: protein motif:HMM:NF025881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO04148.1"
                     /translation="MTDEDNNSANDSGAPAQSAGDTKVPDAGTDRVVPQPIEGEMKKS
                     YIDYSMSVIVARALPDARDGLKPVHRRILYSMYDMGLYHNKAHKKSARVVGECFAAGT
                     RVLTERGLIPIEEVQLGEKVYTQSGRAQVTELYEMPERALLRITLENGVSVVATPSQP
                     FKVIGRGLSYEWKEAKDLSPSDHVVMRLDYPDDVPFVALSKWKGKDKQLNENIAYLIG
                     QFLADGHIEKARSNSSSHKGVFNFFSSSVEVIEHIHGLLKNEFDYESTIQCRCANQDE
                     YLPGQPIMHWIRISDNDLNDYLANNFGIDWTWGVQTKRIPELFYRSPKAVIGALLSGL
                     MDG"
BASE COUNT         1014 a         1040 c         1159 g          664 t
ORIGIN      
        1 atgtcggtgg tcaacgcccg ctcggaatgg ctcgaggtca gggtgcgccg cgagggcaag
       61 gagcactacc tcaggtgcga aaagggcgtg gtgaccgtcc ccctcaggga actgggcccc
      121 gccgagggca gcgggaccac catcaccttc atgcccgaca ggaccatctt cgagacggtc
      181 cagttcgagt acgacgccct ggcggagaag ttcaaggacc tggcgtacct caacaggacg
      241 gccgtgataa catttaggga cgaggcctct ggaaggcagg accgcttcca tttcgaaggg
      301 ggcatcaccg agttcgtgga ggacctgaac cataacaaga ccaagctgca cgacaaaccc
      361 atatacatct acgcggagaa ggacaacgtg atcgtcgagc tggccatgca gtacacggac
      421 tcctactcgg agaacatcta ctccttcgtc aacaacatca acaccgcgga gggcggcacc
      481 cacatggtcg ggctgcgctc cgcgctgacc cgggcgatga acgactacgc caagaagaac
      541 aacatgctga aggggggcct ggacgcgctg agcggggagg atgtcaggga aggccttacg
      601 gccatcctga gcatcaagat cccggagcca cagttcgaag ggcagacgaa gaccaagctg
      661 gggaacagcg aggtgaaggg catagtggac agcctggtgt tcgagaagct gaccgagttc
      721 ctgatggaga accccaaggt ggccgcggtc tgcatcgagc gggccgtcct cgctgcccag
      781 gccagggagg ccgcccggaa ggccagggac ctcacccgcc ggaagggcgt cctggagagc
      841 gccgcccttc ccgggaagct cgcggactgc agcgagaggg acccggcgag gtcggagctg
      901 tacatcgtgg aggggaacag cgcaggtggc tgcttctcag gggataccaa ggtcgccctg
      961 gtcgacggga gggagctctc gctagaagaa ctggtgaaag aacaggcaga gggaaaggag
     1021 cacttctgtt acacgatcag gaagaacggt acgatcgggg tcgagaaggc gataaacgcg
     1081 cgcaggacca aagttgatgc accggtcatc agggtgaccc tggacaatgg cgaggtcatc
     1141 acctgcacgc ccgatcacca gttcatgatc agggatggag ggtatttgga agcgcggtca
     1201 ttgtaccccg gagtctcatt gatgccgctt tatcggaaat cagctgatag gtcacagaag
     1261 aacatcgcaa ttgacgggca tgaaatggtc tgggacccta attcagaatc ctggcttttc
     1321 actcatatgt tggcggatcg gtacaatcga tgggtgggga cctactcggt ccaggatggt
     1381 tctcattgtc accctattga tttcaacaag cggaataaca acccaacaaa gctggtacga
     1441 atggacgtgg acgatcatct cgcacatcac agatcccacg tcgaaaaaac cctgcatagg
     1501 aaggacacca tggagaaatg ccgggtttta aaacaacaac caggatttga agccagaatg
     1561 agcgaccgca tgagagcagg ctcaacagcc gctatcctat caagaaatgc gaaaaaacaa
     1621 tgggcaaatc ccggctacaa gaatcacatg atctccagat ggataaaatt ctatggctcg
     1681 aatccagatc atcaacttgc aaatcgtgaa ttattaatgg cagaggcaaa aaaatactgg
     1741 atcacgcccg gacatcgaaa agaacagtcg attagaaaaa cgatcctgta cagtaataat
     1801 ccgggtttga gagaaaacct atcggcaatc gcccaagagc aatgggcaga ccctaaatta
     1861 agagaatggc gtagccaaac gaccaaagca caatggaccg aggaattcag gaaaaagagg
     1921 aaagccgctc tctatgccaa tctctatgaa accagttaca gaaagacgat atccgttctc
     1981 actcagtacc tagacacaaa tgggcatgtg gacgtcggtc gctatgaaga acatcgtata
     2041 tcgatagggg acgaatctct attgaagttc gatacgttct gtgaaagata ttaccaaaat
     2101 gatgtggtgg ctgctgacga agctatcgca gccttcaatc acaaggtcgt catggtggag
     2161 gatatcggcg aacgtatgga tgtctacgac atcgaggtgc cgcacagcca caacttcgcc
     2221 ctggcgagcg gtgtttttgt tcataacagc gccaagatgg gccgcaaccg ggagttccag
     2281 gccatccttc cgctgagggg gaagatcctc aacgtcgaga aggcaaggat ggacaggatc
     2341 ctcaagaacc aggagatccg gaacctcatc accgccatag gggcgaacat cggccccgac
     2401 tgcgacgcca gcaaggcccg ctaccatcgg atcatcatga tgacggacgc cgacgtggac
     2461 ggcgcccaca tccggacgct gctcctgacg ctcttctacc gctacatgcg cccgctcatc
     2521 gacgccggct tcatctacat cgcccagccc cccctgtaca aggtgagcaa gggcggcaag
     2581 gaggtctacg tctatgacga cgaggccctg gaaaggacgc tccaggagat gggcaggaac
     2641 gtgaacctcc agcggtacaa gggcctgggg gagatgaacc cgaagcagct ttgggagacc
     2701 acaatggacc cggagaccag gacgctgaag agggtcaccg tagaggacgc gatgagggcc
     2761 gacgagctgt tcaccgtcct gatgggggac gaggtggagc cccgccggga gttcatcatg
     2821 tcgcacgcgg acgaggtgga gaacctggac atctgaggtg cccaagatga ccgacgaaga
     2881 caataacagt gccaatgaca gtggagcccc ggcccagagc gcgggggaca ccaaggtccc
     2941 cgacgcaggg accgacaggg tcgtccccca gcccatcgag ggcgagatga agaagtccta
     3001 tatcgactac tcgatgagcg tcatcgtggc cagggcgctc ccggacgcga gggacggcct
     3061 caagccggtg caccggcgca tcctgtactc gatgtacgac atggggctct atcataacaa
     3121 ggcgcacaag aagagcgccc gggtggtcgg ggaatgcttc gcggccggca cgcgcgtgct
     3181 gaccgaaagg ggcctgatcc cgatcgagga ggtccaattg ggcgagaagg tctacacgca
     3241 gagcggccgg gcgcaggtca cggagctcta cgagatgcct gaacgtgcac tgctcaggat
     3301 cacgctggag aacggggtct cggtcgtggc cacgccctcc cagccattca aggtgatcgg
     3361 tcgagggctg tcgtatgaat ggaaggaggc caaggacctc tccccgtcgg accacgtggt
     3421 catgcggttg gactaccccg acgacgtccc attcgtggca ctgtccaaat ggaaggggaa
     3481 ggacaagcag ctcaatgaga acatcgccta cctcatcggg cagttcctgg cggacgggca
     3541 catagaaaag gcacgatcca acagttccag tcacaaaggc gtcttcaact ttttctcatc
     3601 gtctgttgag gtgatcgaac atattcatgg gctattgaaa aacgaattcg attacgaatc
     3661 aacgatccaa tgccggtgcg ccaaccagga cgaatatctt cctggccagc cgataatgca
     3721 ttggattaga atttccgata atgacctaaa tgattatctt gcgaacaatt tcgggatcga
     3781 ttggacctgg ggagtacaaa caaagcgtat tccagagcta ttctataggt cgcccaaagc
     3841 cgtcataggt gcgctactgt ccggtttgat ggacggc
//
LOCUS       DAOSYN010000119         3870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_18969_length_3870_cov_4.719790, whole genome shotgun sequence.
ACCESSION   DAOSYN010000119 DAOSYN010000000
VERSION     DAOSYN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18969_length_3870_cov_4.719790"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="PLJ11_05445"
     CDS             <1..1635
                     /locus_tag="PLJ11_05445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177373.1"
                     /note="unique DNA polymerase that is similar to eukaryotic
                     polymerases; forms a heterodimer of the small and large
                     subunits; possesses polymerization and 3' to 5'
                     exonuclease activities; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HOO04149.1"
                     /translation="KAPSHTIFPIGMHGGPQRLMEKAAEKGTIEVELGIRKCQLCGEH
                     RVYYRCPCGGHTVPIGESKVVPVDLAGELEKAKETLGERTILPKLKGEKELRSKDMGP
                     EPLEKGILRRKHEVFVNKDGTIRFDMTDVPLTHFRPREIGLSVAKARELGYESDVHDR
                     PLTDPEQLCELKVQDIVPSISCGDFMVRVAGFVDDLLERFYGLPRYYNAARREDLIGH
                     LAIGLAPHTSGGILCRIIGFTRASVCFGHPFFHAAKRRNADGDEDSVVLLLDALLNFS
                     RAYLPDSRGGLMDAPLVLALRLDPNEVDKEAQNIDVRWEYPLELYRAGMEFRHPREVQ
                     ERMDLVADRIKSLLQYEGFGFTHDTSDIAEGPAESAYKTLESMMDKMTAQLDLAKRIR
                     AVDEGDVVHRVITKHFLPDLIGNLKSFSGQKFRCTKCGESYRRVPLIGHCYCGNKLNL
                     TVYEASVKKYLEVTSRIGRDYGVSEYTQQRIRLIERSITSIFGEATDPPMTLDAFDEG
                     GEEPLEQVNDPDADEAPAEEPPSDVKGKRPLCLDDF"
     gene            complement(1672..2508)
                     /locus_tag="PLJ11_05450"
     CDS             complement(1672..2508)
                     /locus_tag="PLJ11_05450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HOO04150.1"
                     /translation="MHIKMLEEMETKAKFLVTDASPEKVNALRRALIMDVPKMAIDDV
                     EFHLGSSVDAEGNEYESMSPVFDEIVAHRLGLVPIPTDLELFEFRDRCSCGGEGCPSC
                     TIYYSLEKRGPCEVYSGDLNCLGDSKFAPKDQLIPIVRLGERQGILAYAIAELGTGRK
                     HAKWQPTHGVGYKYFPTVTIDQSKCDNGGTCIQACPKHVMGFKDQKVQVIDNDACVLC
                     EDCVKVCRTGAIEVKWSESKFVFEFETDGSLTARVALVTALEALERTFDEFREKVASL
                     EG"
     gene            complement(2520..2903)
                     /locus_tag="PLJ11_05455"
     CDS             complement(2520..2903)
                     /locus_tag="PLJ11_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOO04151.1"
                     /translation="MGKWGIANVFASYNNIIITLTDITGAETITKCTGGMVVKAAKDE
                     SSPYAAMRAAEKVAEVAKEKGIEGIHVKVRAPGGNRSTSPGPGAQAAIRALARAGLRI
                     GRIEDVTPIPHDGTKKKGGRRGRRV"
     gene            complement(2903..3589)
                     /locus_tag="PLJ11_05460"
     CDS             complement(2903..3589)
                     /locus_tag="PLJ11_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOO04152.1"
                     /translation="MGDPKFPRRSFDTPSHPWQGERIKEEALLVKQYGLKSKKELWKA
                     KTILRDLRKQSRDLQARLRTGEAQAKIETENLLRKCARLGLLPIEGTRLDDVLGLSDI
                     ALLERRLQTVVFNKGLASTVGQARQFIVHGHVCIDDQKVTIPGYIVTREDEERIVMNP
                     RSPIADEMHPLKQAQREAAAAKAHREEAAESRKDLGKKKFVKHVPKDVEAAVQDVEDV
                     PADLPTEEVN"
     gene            complement(3592..>3870)
                     /gene="rpsM"
                     /locus_tag="PLJ11_05465"
     CDS             complement(3592..>3870)
                     /gene="rpsM"
                     /locus_tag="PLJ11_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOO04153.1"
                     /translation="RLITTYSEYAPNWAVNRQADYETGDDLHIVGADLDIVLKDDVNR
                     LKMIRCYRGLRHESGHKVRGQRTRSNGRKGLTLGVSKVKTQPAPKKEA"
BASE COUNT          701 a         1240 c         1183 g          746 t
ORIGIN      
        1 aaggccccct cccacacgat attcccgata ggcatgcacg gcgggccgca gcgactcatg
       61 gagaaggcgg ccgagaaggg cacgatcgag gtggagctgg ggatcaggaa gtgccagctc
      121 tgcggcgagc atcgcgtcta ctatcgatgc ccctgcggcg ggcacaccgt ccctattggg
      181 gagtcgaagg tcgtgcccgt tgatctggca ggggaactcg agaaggccaa ggagaccttg
      241 ggggaaagga ccatcctccc aaagctgaag ggcgagaagg agctgaggtc caaggacatg
      301 gggccggagc cgctggagaa ggggatcctt cgcaggaagc acgaggtctt cgtgaacaag
      361 gatggcacca tccgcttcga tatgaccgat gtcccactca cccatttccg acccagggag
      421 atagggctga gcgtggcgaa ggcccgcgaa ctggggtatg agagcgacgt ccacgaccgc
      481 ccgctcacag accctgagca gctgtgcgaa ctcaaggtgc aggacatagt gccctccatc
      541 tcctgcgggg acttcatggt gcgggtcgcc gggttcgtgg acgacctcct cgagaggttc
      601 tatgggcttc cacgctatta caacgccgcc cggagggagg acctcatcgg gcacctggcg
      661 ataggactgg cgccgcacac ctccgggggc atcctctgtc gcatcatcgg cttcaccagg
      721 gccagcgtgt gcttcgggca tccgttcttc catgcggcga agcgccgcaa cgccgacggg
      781 gacgaggaca gcgtggtcct gcttctggac gcccttctca acttctcccg cgcctatctg
      841 ccagacagcc gcggagggct gatggacgcc ccgcttgtat tggcgctgcg attggacccc
      901 aatgaggtgg acaaggaggc ccagaacatc gatgtgcgat gggaatatcc cctggagctc
      961 taccgggcgg ggatggagtt ccgccatccc cgggaggtcc aggagaggat ggacctcgtg
     1021 gcggaccgca tcaagtcgct ccttcagtat gaggggttcg ggttcaccca cgacacctcg
     1081 gacatcgcgg aggggccggc cgagtccgcc tacaagaccc tggagtccat gatggacaag
     1141 atgacagccc agctggacct ggcgaagagg atcagggcgg tggacgaggg ggacgtcgtg
     1201 cacagggtga tcaccaagca cttcctgccc gacctgatcg gaaacctgaa gagcttctcc
     1261 gggcagaagt tccggtgcac caagtgcggg gagagctata ggcgcgtacc cctgatcggg
     1321 cactgctact gcggcaacaa actgaacctc acggtgtacg aggcaagcgt caaaaagtac
     1381 ctggaggtca ccagcaggat agggcgggac tacggtgtgt ccgagtacac ccagcaacgc
     1441 attcggctga tcgagaggtc gatcacctcc atattcgggg aggcgaccga cccgcctatg
     1501 accctggatg cgttcgacga gggaggggag gagcctttgg aacaggtcaa tgaccccgat
     1561 gccgacgagg ccccggccga ggagcccccc tccgatgtca agggcaagcg cccgctatgt
     1621 ctggacgatt tctagggaag aaaaaaggta gaaaaaaggt tagggggtcg ctcacccctc
     1681 gagggaggcc accttctccc tgaactcgtc gaaggtcctc tcaagcgcct ccagggccgt
     1741 caccagagcg accctggcgg tcagagatcc gtccgtctcg aactcgaaga cgaacttgct
     1801 ctccgaccac ttgacctcta tggccccggt gcggcatacc ttcacgcagt cctcgcagag
     1861 gacgcacgcg tcgttatcaa tgacctgcac cttctggtcc ttgaatccca ttacatgctt
     1921 ggggcaggct tggatgcacg tcccgccatt gtcgcacttg gactggtcta tggtcacggt
     1981 cgggaagtac ttgtacccga ccccatgggt cggctgccat ttggcatgct tgcgcccggt
     2041 ccccagttcc gcaatggcat acgccaggat cccctgcctc tcccccagcc tgacgatggg
     2101 gatgagctgg tccttagggg cgaacttgga atcgccaagg cagttcaggt ccccggagta
     2161 cacctcgcag ggcccccgct tctcgaggga ataatagatc gtgcagctcg ggcacccctc
     2221 ccctccgcag gagcacctgt cccggaactc aaagagctcc aggtcggtgg ggatcgggac
     2281 gaggccgagc cgatgcgcga cgatctcgtc gaacacaggg ctcatgcttt cgtactcgtt
     2341 gccctccgca tcgaccgagg agcccagatg gaattccaca tcatcgatgg ccatcttggg
     2401 gacatccatg atcaacgccc ggcgcagggc gttgaccttc tccgggctgg catcggtgac
     2461 caggaacttg gccttggtct ccatctcctc tagcatcttg atgtgcatgg agcgcctctt
     2521 tacacccttc tgccccgccg cccgcccttc ttcttggtac cgtcatgcgg gatgggggtc
     2581 acatcctcga tgcgaccgat cctcaggcca gcacgggcaa gcgccctgat cgccgcttgc
     2641 gcgccagggc cgggggaggt ggagcggttt ccgccaggcg ccctgacctt gacatggatc
     2701 ccctctatcc ccttctcctt ggcgacctcg gccaccttct ctgcggccct cattgcggcg
     2761 tagggcgagg attcgtcctt ggccgccttg accaccatgc cgccggtgca cttggttatc
     2821 gtctccgcgc cggtgatgtc ggtcaaggtg atgatgatat tgttgtagct agcgaaaacg
     2881 ttcgctattc cccacttgcc catcagttca cctcctcggt cggcaggtcc gccggaacgt
     2941 cctcaacgtc ctggaccgcc gcctccacgt ccttgggcac atgcttcacg aacttcttct
     3001 tccccaggtc cttccgggac tcggcggcct cctcgcggtg cgccttggcc gcagcggcct
     3061 cccgctgagc ctgcttcagg ggatgcatct cgtccgcgat cggggagcgg gggttcataa
     3121 ctatcctctc ctcgtcctcc ctggtcacga tatagccggg gatggtgacc ttctggtcat
     3181 cgatgcacac atgcccgtgc acaatgaact gcctggcctg gccaacggtc gaggcaaggc
     3241 ccttgttgaa caccacggtc tgcaatcggc gttccaagag ggcgatgtcg ctgagcccga
     3301 gcacgtcgtc cagccttgtg ccctcgatgg gcaggaggcc cagccgggcg cacttccgca
     3361 acaggttctc ggtctctatc ttggcctggg cctcacccgt cctgagcctt gcctggaggt
     3421 cacgggactg cttgcgcagg tccctcagga tggtcttggc cttccacagc tccttcttgc
     3481 tcttcaggcc atactgcttc accagcaggg cctcttcctt gatccgctcc ccctgccacg
     3541 ggtgggaggg ggtatcgaag ctcctcctcg ggaactttgg gtcgcccatg ctcaggcctc
     3601 cttcttgggc gcaggctggg tcttgacctt ggaaacaccc agggtcagcc ccttccggcc
     3661 attggacctg gtccgctgcc cgcgcacctt gtgcccggac tcatggcgca ggccccggta
     3721 gcatctgatc atcttgaggc ggttcacgtc atccttcagc acgatgtcca ggtctgcgcc
     3781 tacgatatga aggtcatccc cggtctcgta gtcggcctga cgattgacgg cccagttggg
     3841 ggcatactcc gaataggtcg taatgagcct
//
LOCUS       DAOSYN010000120         3852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_19085_length_3852_cov_3.679747, whole genome shotgun sequence.
ACCESSION   DAOSYN010000120 DAOSYN010000000
VERSION     DAOSYN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19085_length_3852_cov_3.679747"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /gene="mptA"
                     /locus_tag="PLJ11_05470"
     CDS             <1..865
                     /gene="mptA"
                     /locus_tag="PLJ11_05470"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HOO04154.1"
                     /translation="QNRYLTNGFKLTRVGVTGVKKPVQVHRDGRNTCLTCVIDVFVDL
                     PSTQRGSHLSRNLEVIAEIVEQGVASPIKGLEVLAADICRKLLERHDYATYAEVRLSA
                     DYFLERSLPTGRRSNESYKLIASATHKRGNGLMKMIGVQVVGMTACPCAMETVDELQG
                     STLKKEGCLPTITHNQRNVTTAMLEVPEEFDVEADDLIEIVESSFSSPTFEILKRTEE
                     AHVVITAHRNPKFVEDVVRDNLAAILNRYPLLPDDTVVTVRSESEESIHKHNAMAERV
                     TTLGELRADLV"
     gene            943..1542
                     /locus_tag="PLJ11_05475"
     CDS             943..1542
                     /locus_tag="PLJ11_05475"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HOO04155.1"
                     /translation="MEVLRRAQENGAVVMLASGNVLPIAYGLSTFIGLRGPVVAENGG
                     IVSYQDRTYALFSNEVPLQAYEHLREVMPEVRRLFTDQWRRTEVALVREVDLDGVREA
                     LRGWPIEVEATGFAIHLMQVGHSKMAGVRKACELLGMDASEVVAFGDADNDERMLREC
                     GVGVAIGNASERAKRAADLVTKGAHAEGVLEGLTSLGIL"
     gene            1582..1764
                     /locus_tag="PLJ11_05480"
     CDS             1582..1764
                     /locus_tag="PLJ11_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04156.1"
                     /translation="MEVLGLCSLCSKPARYSCSLCGRPVCLEHFDRYAHLCYHCSPDK
                     KDERRHRELPDRRLLG"
     gene            complement(1767..2261)
                     /locus_tag="PLJ11_05485"
     CDS             complement(1767..2261)
                     /locus_tag="PLJ11_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO04157.1"
                     /translation="MATSEFDKLMKDGYRLMEEGEYSQALSKFDKAIKADPANPAAYL
                     AKADAAVLVPKVSQEEVIALYRKASELDPENPFILQSWGAFCMDVGLFNDAEACYNKA
                     AQIDPENAIYYYSEFGVEYYKKALVVYEQRLDEQTRAIIAKKALKYLLKSIDLDETSV
                     KKLL"
     assembly_gap    2312..2321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2334..2687)
                     /locus_tag="PLJ11_05490"
     CDS             complement(2334..2687)
                     /locus_tag="PLJ11_05490"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOO04158.1"
                     /translation="MLRSMRQLLRGKIHRATVTAAEPEYVGSIVIDQDLLERADIWAG
                     ERVLVSDVDNGARFETYVVPGRKGSGVISVNGAAAHLVNVGDKVIIMAFELTDRPIPS
                     RVVLVDQGNRFIRNL"
     gene            complement(2746..3534)
                     /locus_tag="PLJ11_05495"
     CDS             complement(2746..3534)
                     /locus_tag="PLJ11_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04159.1"
                     /translation="MLAVKDLSFKYKERPVLEDVDLEIRKGEVIGILGPNGCGKTTLL
                     KLLNRNLHPQEGKVLLEGTDLEGMSKRRIARHIAVVPQSNEIRFAFTVRDIVSMGRMP
                     FMDRFQGESAEDMRIVDEAMERTNIKEFADRAINTMSGGERQRVIIARALAQRPEVIL
                     LDEPTLHLDINHQFEVLDLVHELSRKEGLTVVIVSHDLPMVVKYCDRMVLIHDHRVHA
                     VGPPEEVLTRENMRTVFNIEAVLTYDDMLGSNCVKILGSCSGRR"
     gene            complement(3534..>3852)
                     /locus_tag="PLJ11_05500"
     CDS             complement(3534..>3852)
                     /locus_tag="PLJ11_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOO04160.1"
                     /translation="KRVRLQLLVASALVTAAAVAFVGVIGFVGLVVPHILRILMGPDN
                     RLLLPASLLGGAAFLMICDYISRVIAPNSIGILPIGIVTALIGAPYFIYLLRRRRTEV
                     GWD"
BASE COUNT          695 a         1166 c         1252 g          729 t
ORIGIN      
        1 tcagaaccgg taccttacca atgggttcaa gctgacccgc gtgggggtaa ccggggtcaa
       61 gaagccggtg caggtgcatc gtgacggccg gaacacctgt ctcacctgcg tgatagacgt
      121 cttcgtggac cttccctcga cccagcgagg ctcccacctc tcccggaacc tggaggtcat
      181 cgccgagata gtggagcagg gcgtggccag tccgatcaag ggccttgagg tgcttgccgc
      241 cgatatatgc cgcaagctgc tggagcggca tgattatgcc acctacgccg aggtgcgctt
      301 gagcgccgat tacttcctgg agcgctcctt gcccaccgga aggcggagca acgaatccta
      361 taagctcatc gccagcgcca cccacaagcg tgggaacggc ctgatgaaga tgataggcgt
      421 ccaggtcgtg ggcatgaccg cctgcccctg cgccatggag accgtggacg aactccaggg
      481 gagcaccctg aagaaggagg ggtgcctccc caccatcacc cacaaccaga ggaacgtgac
      541 caccgccatg ctggaggtcc ctgaggagtt cgatgtggag gcggacgacc tcatcgagat
      601 cgtggagtcc tccttctcca gcccaacgtt cgagatcctc aagcggacgg aggaggcgca
      661 tgtggtcatc accgcccacc gcaaccccaa gttcgttgag gacgtcgtgc gggacaatct
      721 ggcggccata ttgaaccgct accccctgct tccagacgac actgtggtga ccgttcggag
      781 cgagagcgag gagtccatcc ataagcataa cgccatggcc gagcgggtca ctaccttggg
      841 agagctccgc gctgacctgg tgtgaggatg cgctacaagg cggtggtgtg cgatgtggac
      901 ggcaccatca cggacagctc caaacgaatc tagtgcctgg ggatcgaggt cctcagaagg
      961 gcccaggaga acggggccgt ggtgatgctg gcgagcggga acgtgctgcc gatcgcctat
     1021 gggctctcca ccttcatcgg cctccggggc ccggtggtgg cggagaacgg gggcatcgtc
     1081 tcctatcagg accggacgta cgcgctcttc tccaacgagg tcccgctgca ggcgtacgag
     1141 catctgcgcg aggtgatgcc ggaggtaagg cgcctcttca cggaccaatg gcgccggacc
     1201 gaggtcgcat tggtccgcga ggtggacctg gacggggtca gggaggccct gcggggatgg
     1261 cccattgagg tggaggccac gggcttcgcc atccacctga tgcaggtggg gcatagcaag
     1321 atggcggggg tgcggaaggc ctgcgagctc ctagggatgg acgcctcgga ggtcgtggcg
     1381 ttcggggacg cggacaatga cgagcgcatg ctccgggaat gcggcgttgg ggtggcgata
     1441 gggaacgcct cggagagggc caagcgagcg gcggacctgg tcacgaaggg cgcgcatgcg
     1501 gagggcgtcc tagaggggct gacgtccctg ggcatcctct gaagggaagc tattactagt
     1561 accgctgaca attggattag catggaggtc ttgggcctct gctcgctgtg ctccaagccg
     1621 gccaggtaca gctgctcgct ctgcgggcgg ccggtgtgcc tggagcactt cgacaggtat
     1681 gcgcatctgt gctatcattg ctcgccggac aagaaagacg agcgccggca ccgggagctg
     1741 cccgaccggc gcctgctcgg ctgagttcag aggagcttct tgaccgaggt ctcgtcgagg
     1801 tcgatggact tgagcaggta cttcagcgcc ttcttggcga tgatcgcccg cgtctgctcg
     1861 tcaagacgct gctcatagac caccaacgcc ttcttgtaat actccacccc gaactcggaa
     1921 taatagtaga tggcgttctc gggatcgatc tgggcggcct tgttgtagca tgcctccgca
     1981 tcgttgaaga gccctacgtc catgcagaag gcgccccagc tctggaggat gaaggggttc
     2041 tccggatcaa gctcggaggc cttcctatag agagcgatga cctcctcctg ggagaccttg
     2101 gggaccagca ccgctgcgtc cgccttggca agataggccg cagggttggc cgggtcggcc
     2161 ttgatggcct tgtcgaactt ggaaagggcc tgggagtact ccccttcctc catcagcctg
     2221 tagccgtcct tcatcaactt gtcgaactcg ctggtcgcca ttggatccct ttagccgagt
     2281 attggatggt tggagataaa gctttcttgg gnnnnnnnnn nccgaggtgg gggctacagg
     2341 ttccggatga accggttgcc ctggtccaca agcacgaccc ttgatggtat ggggcgatcg
     2401 gttagctcga aggccatgat gatgaccttg tcccctacgt tcaccaggtg ggcggccgcc
     2461 ccgttcacac tgatgacgcc tgaccccttc cttcccggga cgacgtacgt ctcgaagcgg
     2521 gcgccgttgt ccacgtcgga caccaggacc ctctccccgg cccagatgtc cgccctttcc
     2581 aggaggtcct ggtcgatgac gatgcttccc acgtactccg gctcggcggc ggtgaccgtg
     2641 gcccggtgga tcttgccccg caggagctgc cgcatggacc taagcaagaa aagcctcctc
     2701 ttgaaccttt ccccgacggg tgcctggagc gatcgacgcc cggtgtcagc gcctacctga
     2761 gcatgagccg aggatcttta cgcagttgga gccgagcatg tcgtcgtagg tcagcaccgc
     2821 ctcgatgttg aacaccgttc gcatgttctc tctcgtcagg acctcctcag gggggccgac
     2881 ggcatgcacc ctatggtcat ggatgagcac catgcggtcg cagtacttga cgaccatggg
     2941 caggtcgtgg gagacgatga ccacggtcag cccctccttc cgggagagct cgtggaccag
     3001 gtccaggacc tcgaactggt ggttgatgtc caagtgcagc gtcggttcgt cgaggaggat
     3061 gacctccggc ctctgggcca gggccctggc gatgatcacc ctctgccttt cccccccgct
     3121 catggtgttg atcgccctgt cggcgaactc cttgatgttg gtcctctcca tggcctcgtc
     3181 cactatgcgc atgtcctcgg cggactcgcc ctggaagcgg tccatgaagg gcatccttcc
     3241 catgctgacg atgtcgcgca cggtgaaggc gaaccggatc tcgttgctct gcgggaccac
     3301 cgcgatgtgc ctggctatcc tccgcttgga catcccctcc aggtcggtcc cctccagcag
     3361 gaccttgccc tcctgagggt gaaggttgcg gttgaggagc tttagaaggg tggtcttccc
     3421 gcatccattc ggccctagga tgccgatgac ctcccccttc ctgatctcca ggtcgacatc
     3481 ctcgagcact ggcctttcct tgtacttgaa ggagaggtct ttgacggcca gcatcagtcc
     3541 caccccactt cggtgcgcct tctgcgcaac aggtagatga agtagggggc gccgatgagg
     3601 gcggtcacga tgccgatggg caggatcccg atgctgttgg gcgcgatgac cctggagatg
     3661 tagtcgcaga tcatgaggaa cgccgcgccg ccgaggaggg aggcggggag cagcagccgg
     3721 ttgtccggtc ccatgaggat gcgcaatatg tgcgggacga cgagcccgac gaagccgatg
     3781 accccgacga aggccactgc cgcggcggtc accagggcgg aggcgacgag cagctgcaag
     3841 cggacccttt tc
//
LOCUS       DAOSYN010000121         3842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_19140_length_3842_cov_2.870346, whole genome shotgun sequence.
ACCESSION   DAOSYN010000121 DAOSYN010000000
VERSION     DAOSYN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19140_length_3842_cov_2.870346"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3547
                     /locus_tag="PLJ11_05505"
     CDS             <1..3547
                     /locus_tag="PLJ11_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO04161.1"
                     /translation="QRRTGYFNSRALAMAARGLSGLISNGGKMQMICSVELDAADMEV
                     YRDPSKHAKFIEGQAKLVAKMMEEPYDQLEKDRFSILAEMLSRGLLEIKVAFPDGSGI
                     FHEKAGIFKDEVGNFVAFNGSDNETPGGWLHNTESFHVFRSWSENDHILSEQRIFDQL
                     WNDKFPKVTVVPLPKAVQDSILRYKRYDDGQWWTTLDRPDGEEQQVDVPNWVWTPELA
                     YIFESPRLWNRSDFAFAETGIKPFEHQDYVANMVMSQWPPRCMLCDEVGLGKTIEAGL
                     ILKGLMASGKTDRVLILAPKNILKQWQLQMLSKFNLTAWILEGNFVLGPQPDPDLPVP
                     KEPVDIINPFRTKPIMLVSSQLVRSEARLAQATQVEYDLIFLDEAHHARASRGVGGRR
                     EPNLLLQAMESLKLQTQGLIFMTATPIQLSRADLWDLLMVLEIPGEWQDEGSFDRFFE
                     EINKKHPDWNFLLGMARSSVNAYGIDRGSVRAIGAKYPMVDVYRLEQLLKDGNGNQVR
                     ELSAQEVEVLKLLLYRHTPAYKLIFRNTRELLREYHRQGKFSERIAEREPLPPIVISL
                     DGSEDDPRSELGLYSRIDKYVRDYYDSYESVRKGLGFIMEVYRKRLTSSFYAITKSLE
                     RRRDKLQNALRTGDLHELIPNEFDEEDEDEYEGAGELGDLRLNQVRAIITEELEYLNS
                     FLNDLRSLRTDTKTDAMDELLRKLLRSGTKQVIMFSQYMDTVEHILDYLRPKYGDKLG
                     SYSGSGGRYWDGEKWATCTKQKIQEKFRNDDDPLCILVCTDAASEGLDLQTCDTLINF
                     DIPWNPMRIEQRIGRIDRIGQRSPKVFIHTYFYKDTVEEEVYRRCLQRIDFFRTTLGN
                     LQPILAQAQRMIRQGAMARNKADGAKVIDEMDRELQRSIAELDEEIRIDDLMNHYEPS
                     YPVKKGMVPISQQELCSNIAPILEGLGWKKAENVWSKDGSRVTFDPSIIDSKDATTEL
                     LTPLSNLTHHIGVLPVMVDEIPIDGTGQLIKASVDGQTVFMVKDANGFRLIRTLTDIR
                     SSDGRYHPTKEACIREVQYFLRQRKRENLDTALGIWNNRLEGWKVRVGMYLNSVFEWR
                     MSEIGAGIESFDEDRVWKAWNDYLGEAERAQTKQLASLIGYAPPIGKIRTKRGRKPSS
                     SPRSSEKEGRMLIEYQNIIDRIERTKISLKEIEGR"
BASE COUNT         1001 a          958 c         1109 g          774 t
ORIGIN      
        1 tcagagaagg accggctact tcaacagccg cgccctggcc atggccgctc gtggtctgag
       61 cggtctcatc agcaacggcg gaaagatgca gatgatatgc tccgtggaac ttgacgccgc
      121 ggacatggag gtctaccgcg acccatccaa gcacgccaag ttcatagaag ggcaggcgaa
      181 gctggtggcc aagatgatgg aggagccgta tgaccagttg gaaaaggacc gcttctccat
      241 tttggcggaa atgctctctc ggggtcttct ggagatcaag gtggccttcc cggacggctc
      301 cggcatcttc cacgagaagg ctggaatatt caaggacgag gtagggaact tcgtggcgtt
      361 caatggaagc gataacgaaa cgccaggtgg ttggcttcac aacaccgaat cgttccatgt
      421 gttcaggtcg tggagcgaga acgaccacat actgtccgag caaagaattt tcgatcaatt
      481 atggaatgac aaattcccga aggtcaccgt cgtacctctg cccaaggcgg ttcaggattc
      541 cattctccgg tacaagcgtt atgatgatgg ccaatggtgg actacattgg acaggcccga
      601 tggggaggaa cagcaagtgg acgttcctaa ttgggtctgg acccccgagc tcgcttacat
      661 tttcgaatcc cctcggctat ggaacaggtc tgacttcgct ttcgctgaga ccgggataaa
      721 accgttcgaa catcaggact acgtggccaa catggtcatg tctcaatggc cgcccaggtg
      781 catgctctgc gacgaggtag gtctggggaa gaccatcgag gccggactga tcctcaaagg
      841 attgatggca tcgggcaaga ccgatcgagt tttgatactg gccccaaaga acatcctgaa
      901 gcagtggcag ctgcagatgc tgagcaagtt caacttgacc gcctggatcc tggaaggcaa
      961 cttcgtgctc ggaccccagc ccgaccccga tctgcccgtt cccaaggagc cggtggacat
     1021 catcaacccg ttccgcacca aaccgatcat gctggtctcc agtcagcttg ttcgaagcga
     1081 ggctcgcttg gcccaggcca cacaggtgga gtacgacctc atattcctgg acgaggccca
     1141 tcatgcccgc gcctcccggg gcgtaggcgg aagaagggaa cctaacctcc tgctacaggc
     1201 catggaatca ttgaagctgc agacccaagg cctcatcttc atgaccgcca ctcccattca
     1261 gctctccagg gccgacctat gggaccttct catggtattg gagatccccg gggaatggca
     1321 ggacgaaggc tcgttcgaca ggttcttcga ggagatcaac aagaagcatc cggactggaa
     1381 cttccttctg ggcatggcta ggtcttctgt caatgcctac ggcatcgata ggggaagcgt
     1441 aagggccatc ggtgccaagt atcccatggt ggacgtgtac cgcttggaac agctcctgaa
     1501 ggatgggaat gggaatcagg tcagggaact gagcgctcag gaggtcgagg tgctcaagct
     1561 cctcctgtac cggcacactc cagcttacaa actgatattc aggaacacca gggagctgtt
     1621 aagggaatac caccgccaag gcaagttctc ggaaaggatc gccgaaaggg agccgctccc
     1681 gcccatcgtc atatccctgg atggcagcga ggatgaccct cgctccgagc tcgggctgta
     1741 ttccaggata gacaagtatg tccgggacta ctacgacagt tacgagtcgg tcagaaaagg
     1801 cttgggcttc atcatggagg tctataggaa aaggctgacc tccagcttct atgccataac
     1861 caagtcattg gagcggaggc gggacaagct gcagaacgca ttaaggaccg gtgatctgca
     1921 cgaactcatt ccaaatgagt tcgatgaaga agacgaggat gagtatgagg gtgcggggga
     1981 gctcggggac ctgagattga accaggtccg agcgatcatc acggaagagc tggaatatct
     2041 gaactccttc ctcaacgacc tgcgatctct gcgcacggac accaagaccg atgcgatgga
     2101 cgaactgctg agaaagcttc ttcgttctgg gaccaagcag gtcataatgt tcagtcagta
     2161 catggacact gtcgaacaca tcctggacta tctgcgtccc aagtacgggg ataagctggg
     2221 ctcgtattcc ggttccggtg gaaggtattg ggacggggag aaatgggcca cctgtaccaa
     2281 gcagaagata caggagaagt tcaggaacga cgatgatccc ctgtgcatcc tggtatgcac
     2341 ggatgcggcc agtgaagggt tggaccttca gacatgcgac actttgatca atttcgacat
     2401 cccctggaac cccatgagga tagagcagcg gataggcagg atagatcgca taggtcagcg
     2461 gtcgccaaag gtcttcattc acacctactt ctacaaggac acggtggagg aggaggtata
     2521 ccgcagatgt ctgcaacgca tcgacttctt ccgcacgacc ctgggtaacc tgcagccaat
     2581 actggcccag gcacaaagga tgatccgtca gggggccatg gccaggaaca aggcggatgg
     2641 tgcaaaggtc atcgatgaga tggacaggga actgcagagg agcatcgcgg aactggatga
     2701 agagatccgc atcgacgatc tcatgaacca ttacgaacca tcatatccgg tcaagaaagg
     2761 aatggtaccg atctcccaac aggaattgtg ttcaaacatc gctccgatcc ttgaggggtt
     2821 gggatggaag aaagccgaga acgtatggag caaggacggc tctcgagtaa cgttcgatcc
     2881 tagtataata gattccaagg atgccacgac cgagctgctg acccccctct ccaatctgac
     2941 ccatcatatc ggtgtacttc cagtcatggt cgatgagatc cccatcgatg gcacaggtca
     3001 attgatcaag gcatccgtcg atggacagac cgtgttcatg gtgaaggatg ccaacggttt
     3061 ccgcttgatc aggacattga cggatatccg ctcatcggat gggcggtatc accccaccaa
     3121 ggaagcatgt atacgggagg tccaatactt cctccggcag aggaagaggg agaacttgga
     3181 cacagccctt ggaatatgga acaacaggtt ggagggctgg aaggtcagag tggggatgta
     3241 cttgaacagt gtattcgagt ggaggatgag cgagataggg gctggcatcg aatcgttcga
     3301 tgaggatagg gtttggaagg cctggaacga ttatctcggg gaggcggaaa gggcgcaaac
     3361 caaacagctg gcgtcactga taggttatgc acctcctata gggaagatcc gcaccaagag
     3421 gggcagaaag ccatcatcaa gccctcgctc ttccgaaaag gagggccgga tgctcattga
     3481 gtaccagaac ataatcgata ggatcgagcg tacgaaaatt tcattgaagg agatcgaagg
     3541 gcggtaaacc gtggaatgac attatagctc gaggggacga caatgggtga gatcaaatta
     3601 ttcagaactt caaaggacaa agtggaggag atcaaggggg actcagttgc cctcgagaaa
     3661 tcattacaaa tactgattgg gctttgttgc aaaattcagc cagacactat gccaacctca
     3721 ggcaattaga tcctaattga tctatattgt taccaatatt gattacaaaa tatttaagtg
     3781 tggtccgatc cattcgccag ctgtccaaag aaggtgactg gattggttgc cacggtcgta
     3841 ga
//
LOCUS       DAOSYN010000122         3743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_19778_length_3743_cov_2.963666, whole genome shotgun sequence.
ACCESSION   DAOSYN010000122 DAOSYN010000000
VERSION     DAOSYN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19778_length_3743_cov_2.963666"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..908)
                     /locus_tag="PLJ11_05510"
     CDS             complement(120..908)
                     /locus_tag="PLJ11_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO04162.1"
                     /translation="MPLAAAFRSFFRKTLAITELEARKLRHDPWDLFTRAVQPVLWLV
                     VFGQVLAQVKAIPTGDMPYLDFLTPGILAQSVLFISIFYGISIVWERDLGIVQKFLVS
                     PTPRTALVTGKALAAGLRSFTQAVIVLILAMALGVDVVLAPLELLTVVLVVLLGAAIF
                     ATLSLIIACLVKTRERFMGIGQMVTMPLFFASNAIYPIAMMPGWLQVISYGNPLTYVV
                     DALRGSMVVGGASEFGLLLDLGLLAAIAVALILIAGRVYPRVGM"
     gene            complement(909..1712)
                     /locus_tag="PLJ11_05515"
     CDS             complement(909..1712)
                     /locus_tag="PLJ11_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO04163.1"
                     /translation="MNDRTMMVRTERLTHRFGDLTAVDSLDLEVAEGEVFGLLGPNGA
                     GKTTTLKMLCTLLPPSAGRAFVAGHDVVREAATVRKEIGYVPQLLSAEASITGYENLL
                     IFGKLHDVPRAELEERARKALERMGLSEFGDRLVRTYSGGMVRRLEIAQSSLHRPKVL
                     FLDEPTIGLDPVARRAVWEMVQGLRETFGTTLFLTTHFMDEADALCDRIGIMHHGRLI
                     ALGSPEELKDMTGMSTLDEAFISLTGNALETSGNVRDIQRDRRTARRLG"
     gene            1974..3422
                     /locus_tag="PLJ11_05520"
     CDS             1974..3422
                     /locus_tag="PLJ11_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04164.1"
                     /translation="MVRKGIVGVVVLLLAMGLGRWRHRPQAAPQDWVLEGQITGERSA
                     FVSAELPDGRTFVALRYNVTGEVFRNDTWLLDPADMTWERLADSPVGTEGSAGAYADG
                     KVYVFGGLLSNFSYITTVLIYDLATDEWSTGPALSGTGVFMRAATIDGTCILIAGGWH
                     HGFEKCYAFDTRTGAFEAAPDMPEGRAAGAMVAYGDSVYYFGGWDSSYAIQRSVFGYT
                     LIGDYWWSGTPLPEARASMAGVAGSDGVVYLIGGTNNVGWYEANVAEVWGYDLSSGGT
                     IIVPAVAEPARYGAAFETADGRIVFFGGHNGTEGSTDIWSLKVWDIEAILSSGTVAQG
                     GSVQLSLSLTTYFAPEEGWYGTAYLMAGDITYALYDISALGVKGILELPISEGMPAQD
                     YLLVLDVGADWDYDLQVEPLPLTVTEAPSIADQLQEMQEQNEALQEQLEAAQAELADA
                     TDAKLDATIGYVILVLVLVTLVVGVVGLVRRK"
BASE COUNT          650 a         1202 c         1250 g          641 t
ORIGIN      
        1 gtgaacctgt tctccttgtt ccagacgctc tccccatgcc tcaaaagtac gaccttgatc
       61 attgtcctcc catccagatg acagggggag gtcaaaactg tttctacagg ccagcgcgct
      121 cacatgccca cccgcgggta caccctcccg gcgatgagga tgagggcgac ggcgatggcc
      181 gcgagcagcc ccaggtccag gaggaggccg aactcgctcg cgcccccgac gaccatggac
      241 ccccgcagcg cgtccaccac gtaggtcaat gggttcccgt acgagatcac ctgcagccag
      301 ccgggcatca tcgcgatggg gtagatggcg ttgctcgcga agaacagggg catggtcacc
      361 atctgcccta tgcccatgaa ccgctccctc gtcttcacca ggcacgctat gatgagcgaa
      421 agggtggcga agatggccgc ccccaggagg acgaccagga cgacggtcag cagctccagg
      481 ggggcgagca cgacatccac ccccagggcc atggccagga tgaggacgat gacggcctgg
      541 gtgaaggagc gcagcccagc cgcgagcgcc ttcccggtca ccagggcggt gcggggcgtc
      601 ggggacacca ggaacttctg gactatcccg aggtcccgct cccagacgat cgagatgccg
      661 tagaagatgg agatgaagag gacgctctgc gccaggattc ccggggtcag gaagtccagg
      721 tagggcatgt ccccggtggg gatggccttc acctgcgcca gcacctggcc gaagacgacg
      781 agccagagca ccggctgcac cgcccgggtg aacaggtccc aggggtcgtg ccgcagcttg
      841 cgggcctcca gctcggtgat ggccagggtc ttcctgaaaa aggagcggaa cgcggcggcc
      901 agcggcacct accccagcct cctggcggtg cggcggtccc gctggatgtc ccgcacgttg
      961 ccgctcgtct ccagggcgtt ccccgtcagg ctgatgaacg cctcgtccag cgtgctcatt
     1021 cccgtcatat ccttcagctc ctccgggctt cccagggcga tgaggcgtcc atggtgcatg
     1081 atgccgatgc ggtcgcacag cgcgtccgcc tcgtccatga agtgcgtggt caggaagagc
     1141 gtggtcccga atgtctcgcg cagcccctgc accatctccc acaccgccct ccgggcgacc
     1201 gggtccagtc ctatcgtggg ctcgtccaag aaaaggacct tggggcggtg cagggaggac
     1261 tgcgcgatct ccagccttct caccatccct cccgagtacg tgcggacgag ccggtcgccg
     1321 aactcgctca gccccatgcg ctccaatgcc ttcctcgccc tctcctccag ctcggccctg
     1381 ggcacgtcat gcagcttgcc gaagatgagc aggttctcgt acccggtgat gctcgcttcc
     1441 gcggagagga gctggggcac ataccctatc tccttccgga cggtcgccgc ctctcgcacg
     1501 acgtcgtgcc cggccacgaa ggcccttccg gccgagggcg ggagcagggt gcagagcatc
     1561 ttgagcgtgg tcgtcttccc cgcgccgttc gggcccagga gcccgaagac ctcgccctcg
     1621 gcgacctcga ggtccaggga gtccacggcg gtgaggtcgc cgaagcggtg ggtcaggcgc
     1681 tccgtcctga ccatcatcgt cctgtcgttc atgatgtacc ttggccgcag ccgatggacc
     1741 gcatcgcgga gcgcattatt tacgggtttg ccgatgggaa agagggcggc gcgggctcga
     1801 gggaggtccc cttatcccca tatggagccc cgtcccatgt ccttgacggg cggtcgggcc
     1861 ctaagcctcc tcaggtaggg ccgccgatag cagggcagga agcattatat gccgatgaaa
     1921 aaaatagtaa cgcgtccccc gtgggcaggg gacgttcata aaggttgatc gatatggttc
     1981 gcaaggggat agttggggta gtcgttctat tgctggcgat gggcctgggc cgatggcgcc
     2041 atcggcccca ggccgcccct caggattggg tcctggaggg gcagattaca ggggaacggt
     2101 cggcgttcgt cagcgccgag ctgccggacg gccggacctt cgtggccctg aggtacaacg
     2161 tcacgggaga ggtatttcgc aatgacacct ggctgctcga ccccgccgat atgacatggg
     2221 agcgcctcgc cgattcgccg gtgggcaccg agggaagcgc aggggcctat gcggacggga
     2281 aggtgtatgt gttcggcggg ttgctttcca actttagcta catcacgacc gtgctgatct
     2341 atgacctggc cacggacgaa tggagcaccg gcccggcact gtcaggtacg ggggtgttca
     2401 tgagggcggc gaccatcgac ggcacgtgca tcctgatagc gggcggctgg caccacgggt
     2461 tcgagaaatg ctatgctttc gatacaagga caggggcgtt cgaggccgca ccggacatgc
     2521 cagaggggcg cgccgctggg gccatggtcg cctacgggga ctccgtctat tacttcggcg
     2581 ggtgggactc ctcctatgcc atccagagga gcgtgttcgg atacaccttg atcggtgact
     2641 attggtggtc gggcaccccc ctccccgagg cccgggcgag catggccgga gtggccggga
     2701 gcgatggcgt cgtctacctg ataggcggga cgaacaacgt cggttggtac gaggccaatg
     2761 tggccgaggt ctggggatac gacctctcct ccggagggac catcatcgtc ccggccgtgg
     2821 cggagcccgc gaggtacggg gcggcgttcg agacggccga cggcaggata gtgttcttcg
     2881 gagggcacaa cgggaccgag gggagcacgg acatctggtc gctcaaggtc tgggacatcg
     2941 aggcgatcct gagctcaggg acggtcgccc aggggggcag cgtccagctc agcctctccc
     3001 tcacgaccta tttcgcgcct gaggagggct ggtacggaac ggcgtacctg atggccgggg
     3061 acatcacgta cgccctttac gacatcagcg ccttgggcgt gaaggggatc ctcgagcttc
     3121 cgatctcgga ggggatgccc gcgcaggact acctgctggt gctcgacgtg ggggcggact
     3181 gggattacga ccttcaggtc gagcccctgc ccctgacggt caccgaggcg ccctccatcg
     3241 cagaccagct ccaggagatg caggaacaga acgaggcgct gcaggagcag ctcgaggccg
     3301 cccaggccga gctcgcggac gccacggacg ccaagctgga cgcgacgatc ggctacgtca
     3361 tactggtgct ggtcctggtc accctggtcg tcggcgtcgt cggcctggtg cgcaggaagt
     3421 gagccaaacc ctttcatcct tcttttttta ctcggtccat tatagtgggc aagggtctcc
     3481 ggccatcgga tgattcctgc ccctgggccg tggacgtgag ggccacgacg gagggaaccg
     3541 cgcatcctct catcgaggcc ctccgtgtcg ccggcaacct cggcgcgcgc cgtccgtcgt
     3601 cccgtgatag cggtccctga gccagggagg ccccctccag ctctgtcagg cctccctatc
     3661 agcaaccgct cagtaaagag gatcgtcctt cagtgcctga ggaatccggc cctctccatg
     3721 gcccgatggg ccacggcgcc gct
//
LOCUS       DAOSYN010000123         3722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_19911_length_3722_cov_3.537497, whole genome shotgun sequence.
ACCESSION   DAOSYN010000123 DAOSYN010000000
VERSION     DAOSYN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19911_length_3722_cov_3.537497"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="PLJ11_05525"
     CDS             <1..1367
                     /locus_tag="PLJ11_05525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOO04165.1"
                     /translation="FLAGFSLAGVGLSMLAVVMHFVDGTGRVVFDTGLLVLDDFSGLF
                     MLVFLTVAFLVILASIRYIEDERHKAEYYSLILLATVGMMLVAASTDLFVLYIGIEIT
                     SLSSYALVAFRKKDIKGAEAATKYFIFGGISSAFTLFGISLVYGLTGTTSFQEVGVLV
                     GTLDGYREMLWLALVMVMVGFGFKVAMVPFHSWAPDVYEGAPTTITAMLAAGSKKMGF
                     VAMFKFFLIGMLAIKADWDVVVAILSIVTMTLGNLAALNQTSIKRMLAYSSIAQAGYI
                     LMALPVGTEYAVTGGIFHILTHAFMKGGAFIAVATLATVAIGDRISDYKGLAKRSNFM
                     AFSITILLFSLAGIPPLAGFASKVVLFSSPIMGTVAGSEWMVWLAVAGIINSAISLYY
                     YARVVKYMYVDELDEGAPSERLKLPKGMAAAVAICVLAVIGIGVFPEYFIQLCQNAAQ
                     ALFA"
     gene            1472..2434
                     /locus_tag="PLJ11_05530"
     CDS             1472..2434
                     /locus_tag="PLJ11_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOO04166.1"
                     /translation="MSECWDSSIKDELQMVEEEIRRSIRSEQPILTEICEYVISSGGK
                     RMRPGVTLLSYLANGGRETDDVVKIAAAFEMIHSATLIHDDINDRSEMRRGRPSAYKR
                     FGLQQALVGGDFLFVKAFAIGGFFDEEIVGLVADACTAIAESEIVQFEKEGQIAPLDV
                     YLRIIEGKTARPIMAGARIGAYLAKAGPDRIHAMGEYGLNIGMAFQIVDDILDIIGKQ
                     GSLGKPLMVDFHEGKATLPLIQAMEDPKVGPRLSKLFRKKRKTNADIEEASALIAMTD
                     TVERARQRAKGYAERALAALEGVQDSGHKQGLIRLATMVVERDY"
     gene            2454..3542
                     /locus_tag="PLJ11_05535"
     CDS             2454..3542
                     /locus_tag="PLJ11_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOO04167.1"
                     /translation="MAKELRYDVVVVGAGPAGSTTARFAAEAGASVLMLERRAKVGVP
                     VRCGELMPSMQEMRAMFPKAGDMEDIFEVPPALVSLRFNTIRIFSPKGTRYEVPFSGY
                     TTYRDRLDQFFVSQATAAGTELLTGQRVTRVEAGAVHTADLDVRAKVIVGADGPLSIV
                     AKAFGLPHSADLCPAVTAQVAGEFEPVPEMYFGTVAPGGYAWVIPKAGGANVGLGVSR
                     KFARMPVSKYFDAFLSSKGLQAGRPMGKVVPMAMPVKRTVAGQAMLVGDAAGQVMAVN
                     GGGIPMARIVGKVAGKAAAAHVLNGTPLSEYEREWRRQVYKPLRTAAHTKVLAELCFG
                     SAWRTDAAMRVLGPRRMNNLIRCRPIFP"
     gene            complement(3539..>3722)
                     /locus_tag="PLJ11_05540"
     CDS             complement(3539..>3722)
                     /locus_tag="PLJ11_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04168.1"
                     /translation="VDAAMALPDPEERKKAFQELQRRSEELMESTITHKREMEWSAKG
                     LLRSVPRAAWAVLRGD"
BASE COUNT          685 a         1119 c         1238 g          680 t
ORIGIN      
        1 ggttcctggc ggggttctcc ctcgcggggg taggcctgtc catgctggca gtggtcatgc
       61 acttcgtcga cggcacgggc agggtggtat tcgacactgg actgctcgtc ctggacgact
      121 tcagcggcct gttcatgctg gtgttcctga cggtggcatt cctcgtcatc ctggcttcca
      181 tcaggtacat agaggacgag cggcataagg cggagtacta ctccctgatc ctattggcga
      241 ccgtgggcat gatgctggtg gccgcatcca ccgacctctt cgtgctttac atcggcatag
      301 agatcacgag cctgtcctcg tatgcccttg tggccttccg caagaaggac ataaaggggg
      361 cggaggcggc cacgaagtac ttcatcttcg gaggcatctc atcggccttc accctgttcg
      421 gcatatccct ggtgtacggc ctgaccggga cgacttcctt ccaggaggtc ggcgtcctcg
      481 tcggcaccct ggacggctac agggagatgc tctggctggc gttggtcatg gtcatggtgg
      541 gcttcggctt caaggtggcc atggtcccgt tccactcctg ggccccggat gtgtacgagg
      601 gcgcgcccac gaccatcacc gccatgctcg cggcgggctc caagaagatg ggcttcgtgg
      661 ccatgttcaa gttcttcctg attggcatgc tcgccatcaa ggcggactgg gacgtagtgg
      721 tcgccatcct gtccatagtc accatgacct tgggcaacct ggctgcactg aaccagacga
      781 gcatcaagcg gatgctcgcc tactcgagca tcgcccaggc gggctatatc ctgatggcgc
      841 tgccagtggg aacggagtac gcggtcaccg gagggatatt ccacatcctg acccacgcct
      901 tcatgaaggg gggcgcgttc atcgcggtag caaccctggc caccgtggcc ataggggaca
      961 ggatatcgga ctacaaggga ttggcaaaga gatcgaactt catggccttc tcgatcacca
     1021 tcctgctgtt ctccctggcc gggatcccgc cattggcagg gttcgccagc aaggtcgtgc
     1081 tgttctcctc cccaataatg gggacggtgg ccggctcgga atggatggtg tggctggcgg
     1141 tcgctggtat catcaacagc gccatatccc tgtactacta cgcccgcgtg gtcaagtaca
     1201 tgtacgtgga cgaattggac gagggagccc cgtccgagag gctgaagctg cccaagggca
     1261 tggccgccgc ggtggccatc tgcgtgctgg cggtcatcgg cataggggtg ttcccggagt
     1321 acttcatcca gctctgccag aatgccgcac aggccctgtt cgcctgagaa cccccttccc
     1381 ctttcctttt tttctaagcg ggaacgctgg acgttcagag ctaaccaaag ccagtaacgg
     1441 ttaagtataa atttaccata tcgagcgaac gatgtccgag tgctgggact cctctataaa
     1501 ggacgagctg cagatggtgg aggaagagat ccgccgcagc atccgttcgg agcagcccat
     1561 actcaccgag atctgcgagt acgtgatctc atccggcggc aagcgcatgc gccccggggt
     1621 caccctcctc tcctatttgg cgaatggggg cagggagacg gacgacgtgg tcaagatcgc
     1681 cgcggccttc gagatgatcc attccgcgac attgatccat gacgacataa acgaccggtc
     1741 ggagatgaga cggggacggc cctccgcgta caagaggttc ggactgcaac aagcgttggt
     1801 gggcggggac ttccttttcg tgaaggcctt cgccatcggc ggcttcttcg acgaggagat
     1861 agtgggcttg gttgcggacg cctgcaccgc catagccgag agcgagatcg tccagttcga
     1921 aaaggagggg cagatcgccc ccttggacgt atatctcagg atcatcgagg gcaagaccgc
     1981 aaggccgatc atggccgggg cccgcatagg ggcgtacctg gccaaggcgg ggccggaccg
     2041 catccacgcc atgggcgagt atgggctgaa catcgggatg gccttccaga tcgtggacga
     2101 catattggac atcatcggaa agcagggttc gctcggcaag cccctcatgg tcgatttcca
     2161 tgaggggaag gccaccttac ccctcatcca ggcgatggag gaccccaagg tcggcccgcg
     2221 cctttccaag ctcttcagga agaagcggaa gacgaacgcg gacatcgagg aggcctccgc
     2281 gctcatcgcg atgacggata cggtggaacg ggccaggcag agggccaagg ggtacgcgga
     2341 aagggcattg gcggccctgg agggggtcca ggacagcggg cacaagcagg gcctcatcag
     2401 gttggcgacc atggtcgtcg agcgcgacta ttgagcgcgg caaggggagg ggcatggcaa
     2461 aggaactgag gtacgacgtg gtcgtggtgg gcgcgggacc cgccgggtcc accaccgccc
     2521 ggttcgcggc ggaggccggg gcaagcgtgc tgatgctcga gaggcgcgcg aaggtgggag
     2581 tcccggtccg gtgcggcgaa ctcatgccca gcatgcagga gatgagggcc atgttcccaa
     2641 aggcggggga catggaggac atattcgagg tcccccccgc cctggtctcc ctgaggttca
     2701 acaccatccg catcttctct cccaagggca caaggtacga ggtgcccttc agcgggtaca
     2761 ccacctaccg tgaccgtctg gaccagttct tcgtctccca ggcaacggcc gcggggacgg
     2821 aactgctgac ggggcagcgc gtcacaaggg tggaggcggg agcggtccac acagcggacc
     2881 tggacgtcag ggccaaggtg atcgtcggcg cggacggccc cctctccatc gtagccaagg
     2941 ccttcgggct gccccattcc gcggacctct gccctgcggt caccgcgcag gtggcagggg
     3001 agttcgagcc ggtgccggag atgtacttcg gaacggtggc ccccggcggt tacgcatggg
     3061 tgatccccaa ggcgggcggg gcgaacgtgg ggctgggagt atcaaggaag ttcgccagga
     3121 tgccggtcag taagtacttc gacgctttcc tgagctcgaa ggggctgcag gccggaaggc
     3181 cgatgggcaa ggtggtcccg atggcgatgc cggtgaagcg caccgtggcc ggtcaggcta
     3241 tgctcgtggg ggatgccgcc ggccaggtca tggcggtcaa cgggggaggg atcccgatgg
     3301 cccgcatcgt gggtaaggtc gccggcaagg cagctgcggc ccacgttctg aatggaactc
     3361 ctctctcgga gtatgagcgg gaatggcgcc ggcaggtcta caagcccctc aggaccgcgg
     3421 cgcacaccaa ggtactggcg gaactgtgct tcggaagcgc ctggcgcacc gatgcggcca
     3481 tgcgggtcct cggcccccgc aggatgaaca acctcatccg ctgccggccg atcttcccct
     3541 aatcgcccct caggacggcc caggcggccc ggggcacgct ccgcaggagc cccttcgcgg
     3601 accattccat ctcccgcttg tgggtgatgg tgctttccat aagctcctcc gacctccgct
     3661 gcagctcctg gaacgccttc ttgcgctcct cgggatcggg gagggccatg gccgcgtcca
     3721 cc
//
LOCUS       DAOSYN010000124         3676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_20206_length_3676_cov_3.231980, whole genome shotgun sequence.
ACCESSION   DAOSYN010000124 DAOSYN010000000
VERSION     DAOSYN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20206_length_3676_cov_3.231980"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1319)
                     /locus_tag="PLJ11_05545"
     CDS             complement(129..1319)
                     /locus_tag="PLJ11_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOO04169.1"
                     /translation="MKAVVLAAGEGNRLRPFTVSRPKVMIPIGNRPILEYVVRALASN
                     GITDIILVVGYMKERIMSFFGNGARFGVQISYAIQEKQLGTAHAALAVRDQVQEEFIM
                     VAGDNIIDAQAIADLLGHGDGMAMLVTESEMPSKYGVVHIEGGRVVDIIEKPDKKVGN
                     IINTGIYRLPPSIFDHLEKGVMAGRSGITHVLHGCLPKIKMEAVRSTGRWMDAVYPWD
                     LVRLNEYAFSMAGQETSGTIEAGVTMRGPVRVGAGSRIRAGCYIEGPVSIGEGCEIGP
                     YVTIFPTSSIGNGVNIAPFTTIDSSIIMNNVSIGSHSHISRAVIDDGCRIMTGLSAPS
                     GLAQVKVEDDFFKVERAGSMVGEFTQIGPRVVLSAGAIVGANCRVGDGAKVSGTLEDR
                     SIVV"
     gene            1455..>1790
                     /gene="gvpD"
                     /locus_tag="PLJ11_05550"
     CDS             1455..>1790
                     /gene="gvpD"
                     /locus_tag="PLJ11_05550"
                     /inference="COORDINATES: protein motif:HMM:NF018756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD"
                     /protein_id="HOO04170.1"
                     /translation="MPDPTRSMLPTEVVQFLEAPGGHSLIVRGNAGTGKTTFALQVAD
                     EFYDRRKVHYLSTRVSDAALLRQFPWLAERTLAREGVGSHPRSGLGRLKGLGASEMVL
                     PRREMTVSIG"
     assembly_gap    1791..1800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1801..2881
                     /gene="gvpD"
                     /locus_tag="PLJ11_05555"
     CDS             <1801..2881
                     /gene="gvpD"
                     /locus_tag="PLJ11_05555"
                     /inference="COORDINATES: protein motif:HMM:NF018756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gas vesicle protein GvpD"
                     /protein_id="HOO04171.1"
                     /translation="ERLYELVEKEGGRALVIIDSIDALADRYELECRALMMALQCDLV
                     EGQGAGALFVLESNDHMLDYLGDGVVECSRVEHERRTVREMALMKLRGCEVQQPLYLF
                     SLKGGRFRCFGADWGRGDGTKGGWTAPVEPPGRMSYGIEDLDSITGGLAPGTVALIEL
                     GTGLPPVVSGILERCLVSSFAKAGRGVLWLPLRKEGGANARALLQGALDDSEMERAVR
                     VMEPASQMELGSERYVMAVEGSRVSDDLRWRNIAYSLDGALQPFLSLMGFDSLESIYG
                     GDVMEGLVDHLASVKRNGGAFVGLTSPTSRSTDRLADLATVHLKVDRLGGTVVLYGIE
                     PFTECFALTVEERPRGGCMSLTPLV"
     gene            2891..3571
                     /locus_tag="PLJ11_05560"
     CDS             2891..3571
                     /locus_tag="PLJ11_05560"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HOO04172.1"
                     /translation="MFKTNLPAMCSVLILGTPGVGMLEFQMGMVKEYVEAGEPVVFVT
                     MDLLPADLLKLMERFGIPLGGLGKGLYIIDYHSGLLGSANGMENGDREKVRRIHDLEG
                     IMFNIASIYEEERRPLRVFMHTLSTMFLYNQPSVVLKFFQISASRIKSEYGTAIVSVY
                     DGVHDEKAVNHLVALADGVIELRFDADLNRMLRVRHMLGMSVPGRWVPFEISPGGEDA
                     PSPVLEWK"
BASE COUNT          655 a         1100 c         1209 g          702 t
ORIGIN      
        1 cgccagccgg ccgttctcga ccatggccac tcccgccgag tcgtagcctc ggtactccag
       61 gcgcttgagt ccctccacca ggacctccga tgccctgcga tgccccgaat agcccatgat
      121 gccacacatc agaccactat gctcctgtcc tcgagggtcc cgctgacctt ggcgccgtcc
      181 cctacccggc agttggcgcc tacgatcgcg ccggcggaca gcaccaccct gggaccgatc
      241 tgggtgaact cccccaccat gctacccgcc ctctccacct tgaagaagtc gtcctccacc
      301 tttacctggg cgaggccgga gggcgcggag aggccggtca tgatgcggca tccgtcatcg
      361 atgaccgccc tggatatgtg ggagtgggag ccgatggaga cgttgttcat gatgatggag
      421 ctgtcgatcg tcgtgaaggg ggcaatgttc accccattgc cgatgctgct ggtcgggaag
      481 atcgtcacgt aggggccgat ctcgcacccc tcgccgatgg aaacggggcc ttcgatgtag
      541 cagccggccc ttatcctgct tccagcgccc acgcgcaccg ggccccgcat ggtaaccccg
      601 gcctcgatgg tcccagaggt ctcctggccc gccatgctga aggcgtactc gttcagccgg
      661 actaggtccc agggatagac cgcgtccatc caccgcccgg tggagcgcac cgcctccatc
      721 ttgatcttgg gaaggcagcc gtgcagcaca tgggtgatgc cggacctgcc ggccatcacc
      781 cccttctcca aatgatcgaa gatgctcggc ggcaggcggt agatacccgt gttgatgatg
      841 ttgcctacct tcttgtccgg cttctcgatg atgtcgacca cccttccgcc ctctatatgg
      901 accaccccgt acttggaggg catttccgat tcggtgacca acatggccat gccgtccccg
      961 tgccccagga ggtcggcgat ggcctgggcg tcgatgatgt tgtcccccgc caccatgatg
     1021 aactcctcct ggacctggtc cctcactgcc agagcggcgt gggcggtgcc cagctgcttc
     1081 tcctggatgg cataggagat ctgcaccccg aagcgcgcgc cattgccgaa gaaggacatg
     1141 atgcgctcct tcatgtatcc caccaccagg atgatgtccg ttatgccgtt ggaggccaag
     1201 gcacgaacga cgtactccag gatgggacga ttcccgatgg gaatcatgac cttgggccgg
     1261 gaaacggtga acggacgcag cctgttcccc tcgccggccg ccagaacgac agctttcatc
     1321 atcaatcctc tgggcatagc catccctgtg gccattaatt aatctttttt catccttcgc
     1381 tgattcccta ggattgataa tggagaggaa agttgtttaa caaacgatgt tggtggggcg
     1441 gagagggaga ccgaatgccc gacccaacgc gctccatgct tccgaccgag gtggtccagt
     1501 tcctggaggc cccgggcggg cattccctga tcgtgcgtgg gaacgccggg acgggaaaga
     1561 cgaccttcgc gctccaggtg gccgacgagt tctacgaccg ccggaaggtg cactatctct
     1621 ccaccagggt ctcggacgcc gccctgctta gacagtttcc ctggttggcc gaaaggacct
     1681 tggcccggga gggagtgggg tcgcatcccc gctccggact gggaaggctc aagggtctgg
     1741 gagccagtga gatggtcctt ccccggcggg agatgaccgt atccatcggg nnnnnnnnnn
     1801 cgaaaggctc tacgagctcg tggagaagga gggagggaga gcgctggtca tcatcgacag
     1861 catcgacgcc ctggcggacc gctacgagct ggaatgccgg gcgctgatga tggccctcca
     1921 gtgcgacctc gtggaggggc agggggcggg cgccctcttc gtcctggaga gcaacgatca
     1981 catgctggac tatttggggg acggggtggt ggaatgcagc cgggtcgagc acgagcgccg
     2041 gacggtccgg gagatggccc tcatgaagct gcggggctgc gaggtccaac agcctctgta
     2101 cctgttcagc ttgaaagggg ggaggttcag gtgcttcggg gccgattggg gccggggcga
     2161 tgggacgaaa ggggggtgga ccgctccagt ggaaccaccc ggtcgcatgt cctacgggat
     2221 cgaggacctg gattccatca ccggaggact ggcccctggg accgtggcgc tgatcgagct
     2281 gggcacaggg ctcccccccg tcgtgagcgg gatactggaa agatgccttg tctccagctt
     2341 tgccaaggcc gggagggggg tgctgtggct gcccctccga aaggaaggcg gggccaatgc
     2401 ccgggcgctc ctgcaagggg cgttggacga cagtgagatg gagagggcgg tgagggtgat
     2461 ggaaccggcc tcccagatgg aactgggcag tgagaggtac gtcatggccg tcgagggcag
     2521 tcgggtctcg gacgacctcc ggtggaggaa catcgcctac agtctcgatg gggccctcca
     2581 gcccttcctg tcgctgatgg ggttcgattc cctggaatcc atctatggcg gggatgtgat
     2641 ggaggggctc gtggaccatc tggcttcggt gaagcgcaac ggcggggcct ttgtcggtct
     2701 cacctcccca acgagccgct ccaccgaccg cttggccgac ctggcgaccg tccatctcaa
     2761 ggtggacagg ctcggaggga cggtggtgct ctacggcatc gagccgttca ccgagtgctt
     2821 tgcgctgacc gtggaggaac gcccccgggg agggtgcatg tccctgaccc cgctggtgtg
     2881 agggagagag atgttcaaga ccaatctgcc cgcgatgtgc tccgttctga ttctgggaac
     2941 ccctggggtg ggaatgctgg agttccagat gggcatggtc aaggagtacg tcgaggcggg
     3001 ggagccggtg gtcttcgtga ccatggatct gcttcccgca gacctcctca aattaatgga
     3061 gcgcttcggg ataccgctcg ggggccttgg gaaggggctc tacatcatcg actatcattc
     3121 agggcttctg ggctccgcta acggaatgga gaacggcgat cgggaaaagg tccggcggat
     3181 ccatgacctg gaaggcatca tgttcaacat tgcctcgatc tatgaggagg agcgaaggcc
     3241 gctgagggtg ttcatgcaca ccctgtcaac gatgttcctc tacaaccagc caagtgtggt
     3301 cctgaaattc tttcagatca gcgcgtcgcg catcaagagc gagtacggta cggccatcgt
     3361 gtccgtctat gacggggtgc atgatgagaa ggccgtgaac cacctggtgg cgctggcgga
     3421 cggtgtcatc gagcttcgct tcgatgcgga cctcaacagg atgctgaggg tgcgccacat
     3481 gctggggatg tcagtgcccg ggcgatgggt cccgttcgag atatcccctg gtggggagga
     3541 cgctccctcc ccggtgctgg aatggaagtg atcagtcctc tacctcttcc agggtcccgt
     3601 acagggcgcg gttgagctcc tgcaccgcac gggcggtcgc tctggagtat aacgggcatt
     3661 cccccttcgg acggac
//
LOCUS       DAOSYN010000125         3672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_20239_length_3672_cov_3.997235, whole genome shotgun sequence.
ACCESSION   DAOSYN010000125 DAOSYN010000000
VERSION     DAOSYN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20239_length_3672_cov_3.997235"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLJ11_05565"
     CDS             complement(<1..512)
                     /locus_tag="PLJ11_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04173.1"
                     /translation="MTENGCPRRLARDRRGQMPFSMIALALIVTSSLSIVLLSEIEEG
                     ATDIGLTMDEIDEMAALSASARQKTEGLAWQALLTTCAGDTVNESAMVERFNHQLSAL
                     MASEFPIVRSGLTAEVNVSGVHLTFMRLALDPGGSPASIVGTYIPAYVGAEGNITIKV
                     ISKDGDLTRN"
     gene            complement(563..1300)
                     /locus_tag="PLJ11_05570"
     CDS             complement(563..1300)
                     /locus_tag="PLJ11_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO04174.1"
                     /translation="MNIIDVLRLDLGDIEREFAQIASVLGNIGLSNYEARAYVALILG
                     THATAEEVAEMAMMPRTSAYKALAGLTAKGFAQEVDGRPTIFYPLDVEELRERTMNEL
                     NGAFAKLSSIKGLLSEKGTPELVYTVHGRKKVLAKIGEMLETARDSFMISSPRMQDIR
                     AELSNQFKEAMGRGVRVTIITEPMVKVPSASEVFRRKGLIATDVIIDGTTAMIATKDL
                     DLGGYSDNPLLAAHLENFLHILMSPSP"
     gene            1464..2927
                     /locus_tag="PLJ11_05575"
     CDS             1464..2927
                     /locus_tag="PLJ11_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO04175.1"
                     /translation="MSEKDGKVVMDKDCPEHGHFHDVVWSDVELYLKCEGLAYDGVGV
                     ENPFIPHAKVCPNECGLCDLHLSHTALANLDLTNRCNMKCPICFANANQAGYVYEPSF
                     EEVVKMMQVLRDSKPVPCPAIQFAGGEPTIYPQFVDVVRKAKELGFAQIQVATNGIMF
                     ANDFEFLKASVEAGLNTIYLQFDGMSDDIYMISRGRKLLDIKMKVIENVRRLEKRPSV
                     VLVPVVVKGVTDSQIGPIFRYALENTDVVRGINFQPVAFTGRINKDELAKQRYTLPDL
                     AKDFEVQTNGQIRKSDWFPVPSVVPISTLASALIGEPKVTFTTHPHCGIATYVFIQDK
                     DHVVPLTSFVDVEPLFRDLYELSKKAERSKMKFPSKVKVYALLRKYMHEERMPEGLDM
                     RTFLKLLGSLMGDSSKGSLAEFSWKMMMIGGMHFQDMYNYDIERVKRCAIHYVVPDGR
                     IIPFCAYNGGPTYREQVEKKFSVPIEEWRRTRGTEHT"
     gene            2939..3112
                     /locus_tag="PLJ11_05580"
     CDS             2939..3112
                     /locus_tag="PLJ11_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO04176.1"
                     /translation="MIVSIEKCMHCGACVGSCPQNAIYLNEVVPEFNEDCNKCGRCVR
                     VCPAGALRMEGKR"
     gene            3109..>3672
                     /locus_tag="PLJ11_05585"
     CDS             3109..>3672
                     /locus_tag="PLJ11_05585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO04177.1"
                     /translation="MSPDYDVIVVGAGPAGSMTAKHAAKKGARVLMIEKRQEIGASLR
                     CAEGVNRKGLEKAGVEVDRRWVAAEIAGAKLVSPGGKVFRIDESQAGNEVGMVLERHL
                     FDKALAADAARAGAEIWLKTAAVGVLKEGDRIIGVKAMREGEPVKLTAGCVVGADGFE
                     SQVARWAGIDTSLKTGDITTCYQYRMAN"
BASE COUNT          781 a         1016 c         1154 g          721 t
ORIGIN      
        1 taattcctgg tgagatcgcc gtccttcgaa atgaccttaa tggtgatgtt gccctccgct
       61 ccaacatagg cggggatgta cgtcccgacg atgctcgctg gggaaccgcc ggggtcgagg
      121 gccaaacgca tgaacgtgag atggacgccg ctgacgttca cctcggccgt cagtccggac
      181 ctcacgatcg ggaactccga ggccatgagc gccgagagct gatggttgaa ccgctccacc
      241 atcgcgctct cgttgacggt gtcacccgcg caggtcgtca gcagggcctg ccatgccagc
      301 ccctcggtct tttgccgggc gctggcggag agcgcagcca tctcatcgat ctcatccatc
      361 gtgagcccga tgtccgtggc cccctcctcg atctcgctga gcaagacgat cgagaggctc
      421 gaggtcacta tgagcgccaa agcgatcatg gagaacggca tctgcccccg acggtcgcgg
      481 gcaagccttc gcgggcaacc attttcggtc atcccttttg gcaagcccgg gagggataaa
      541 aagggggaga gctacagtca ggttacgggg acgggctcat caagatatga aggaagttct
      601 ccagatgggc ggcgaggagc gggttgtccg agtatccgcc caggtcaagg tccttggtgg
      661 cgatcatggc ggtcgtccca tctatgatga catcggtggc gatgaggcct ttcctcctga
      721 agacctcgct tgctgaaggc accttcacca tcggttcggt gatgatggtg acccgaaccc
      781 ccctgcccat cgcctccttg aactggttgg agagttcggc cctgatgtcc tgcatcctgg
      841 gggaggatat catgaacgaa tccctggcgg tctccaacat ctccccgatc ttcgcgagga
      901 ccttcttgcg accgtggacc gtgtagacaa gctcgggggt ccccttttcc gagagcaatc
      961 cctttatcga actcagtttg gcgaacgccc cgttgagttc gttcattgta cgctcccgaa
     1021 gctcctcaac gtccagtggg taaaagatgg ttggacggcc atcgacctcc tgggcgaaac
     1081 cttttgccgt caggcctgcc agtgccttat aggctgaggt gcggggcatc atggccatct
     1141 cggccacctc ctcagcggtc gcgtgggtcc cgaggatgag cgcaacatac gccctggcct
     1201 catagttcga caggccgata tttcccagga cggatgctat ttgagcaaac tccctttcta
     1261 tgtcccctaa atccagtctt aaaacgtcaa tgatgttcat ggccttacat gtcgctgagg
     1321 tcgctacata aatattatat tatcgtgccg aagtaccagt ctggggtgca atcgtgatag
     1381 ctaaagaata ttcattgcca aaagggttgc cgaaaacgac ggggtccctt tgtcccgagt
     1441 gcggcaagat aataacggcg accatgtcgg agaaggatgg caaggtggtc atggacaagg
     1501 actgccctga gcacgggcat ttccatgatg tcgtctggtc cgacgtggag ctgtacctca
     1561 aatgcgaggg gctggcctat gatggtgtcg gggtggagaa tccgttcatc ccccatgcca
     1621 aggtctgccc gaacgaatgc gggctctgcg atctgcatct gagccacacc gcgttggcca
     1681 acctggacct caccaaccgg tgcaacatga aatgcccgat ctgctttgca aacgccaatc
     1741 aggccggcta tgtgtatgag cccagcttcg aagaggtcgt aaagatgatg caggtgctgc
     1801 gggacagcaa accggtccca tgtccggcta tccagttcgc cgggggagag cccacgatct
     1861 acccccagtt cgtcgatgtg gtccggaagg ccaaggaact gggctttgcc cagatccagg
     1921 tggccaccaa cggcatcatg ttcgccaacg acttcgagtt cttgaaggcc tcggtggagg
     1981 cggggctcaa caccatctat cttcagttcg acggaatgag cgatgacatc tatatgatat
     2041 ccaggggaag gaagctgctc gacatcaaga tgaaggtcat cgagaacgtc cggagattgg
     2101 agaagaggcc gtccgtggtc ctagtgcccg tcgtcgtcaa gggcgtcacc gacagccaga
     2161 tagggccgat attcagatac gcgttggaga acacggatgt ggtccgaggg atcaacttcc
     2221 agccggtggc cttcaccggg aggatcaaca aggacgaatt ggccaaacag cggtacaccc
     2281 ttccagacct cgcaaaggac ttcgaggtcc agaccaacgg tcagattagg aagagcgatt
     2341 ggttccccgt tcccagcgtc gttccgatct cgaccttggc ctccgcgctc atcggggagc
     2401 cgaaggtcac gttcaccacg cacccccatt gcggcattgc cacctacgtg ttcattcagg
     2461 ataaggacca cgtggtgccc ttgacgtcct ttgtggatgt ggagcccctg ttcagggacc
     2521 tctacgaact ctccaagaag gcggagaggt ccaagatgaa gttccccagc aaggtcaagg
     2581 tctacgcctt gctcaggaag tacatgcatg aggaaaggat gcccgagggg ttggacatga
     2641 ggaccttcct taaattgctg ggctccctca tgggggacag ttccaagggg tcgcttgccg
     2701 agttctcctg gaagatgatg atgatcggcg ggatgcactt ccaggacatg tacaactacg
     2761 atatcgaacg ggtaaagcgg tgcgctatac attatgtcgt ccctgatggc aggatcattc
     2821 cgttctgcgc ctataacgga gggccgacat accgggagca ggtggagaag aagttctccg
     2881 tgcccataga ggaatggagg cggaccagag gcacggagca tacataagga gggatcaaat
     2941 gatcgtgagc atcgagaaat gcatgcattg tggcgcctgc gtgggctcct gcccccagaa
     3001 cgctatctac ctcaatgaag tagtgccgga gttcaacgag gattgcaaca agtgcgggcg
     3061 atgcgtgcgg gtgtgccccg ccggcgccct taggatggag ggaaagcgat gagccccgat
     3121 tatgatgtga tcgtggtcgg ggccgggccg gcgggcagca tgaccgccaa gcatgcggcc
     3181 aagaaggggg cgagggtgct gatgatagag aagcgccagg agatcggcgc ctctctgcgc
     3241 tgcgccgagg gcgtgaacag gaaggggctt gagaaggccg gggtagaggt ggaccgccgg
     3301 tgggtggcag cggagatcgc gggcgccaag cttgtgtccc cgggcggtaa ggtcttccgg
     3361 atcgatgaga gccaggcggg caacgaggtc gggatggtgt tggaaaggca cctcttcgac
     3421 aaagccttgg ccgcggacgc cgctcgggcc ggggcagaga tatggctgaa gaccgcggcc
     3481 gtgggcgtcc tcaaggaggg cgacaggatc atcggggtca aggccatgcg ggagggggag
     3541 cctgtcaagc tcaccgccgg gtgcgtcgtc ggggcggatg ggttcgaatc ccaggtggcc
     3601 cgctgggccg ggatcgacac ctcactgaag accggggaca tcaccacctg ctaccagtac
     3661 cgaatggcca at
//
LOCUS       DAOSYN010000126         3672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_20244_length_3672_cov_3.139342, whole genome shotgun sequence.
ACCESSION   DAOSYN010000126 DAOSYN010000000
VERSION     DAOSYN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20244_length_3672_cov_3.139342"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1183
                     /locus_tag="PLJ11_05590"
     CDS             146..1183
                     /locus_tag="PLJ11_05590"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOO04178.1"
                     /translation="MRKSAVALLALALVLCSACVPGGTEAAGVAGTDIAGRVVNETGA
                     AFPGVEVRAVNSASRTVYRAYSGPDGNYTMPDDFVQGTYAVSAHFTNYSANITYYDVR
                     VGLGQVLRLNFTMREVLCTLTGFVTNGTTPVYGATVRLIGAGGNYSGVSQLSGQYSIT
                     GVKPGTYTVVASKVGYYPSDPLEPVDMIRGNTTNIDLVLREQPAELTGMVRYDGDGLS
                     GVKVRLSNAQFTAETITDAEGNYTFAQVPSGSYTITFSKDQFLTVEMSVGLSPFEAME
                     LNIEMEYDSANNTQTFLLGLDLAHSLMVVGLGVSLIILAVGLYIDHKVRRRPDVLERE
                     DEGAEKGSEKG"
     gene            complement(1169..1747)
                     /locus_tag="PLJ11_05595"
     CDS             complement(1169..1747)
                     /locus_tag="PLJ11_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOO04179.1"
                     /translation="MTLRELKAPLSEQGARALRAGELVSLSGELLTVRDEAHLRAVRM
                     LRAGEGLPFRLEGMAIYHCGPIVRRDAGWKVIAAGPTTSARLDRLAPELIRLGRPRLI
                     IGKGGMSAEVLEAMREVGCAYLAFPGGAAVVAAEAFPEVIDVHWEDLGMPEAVWHLRA
                     EGLGPMVVAMDAHGRSLFKEVEEGVAKRSALL"
     gene            complement(1744..2613)
                     /locus_tag="PLJ11_05600"
     CDS             complement(1744..2613)
                     /locus_tag="PLJ11_05600"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOO04180.1"
                     /translation="MYSSAIVEEAVVRLLREAECMLPADVRAALSAALENEPEMLPRS
                     QLEVVLRNAAMAAELTVPMCQDTGLPVLFVRGRYDPRLERAIRQGVARATEEVPLRPN
                     TVDPVSRANHGDNLGPGMPPVHFEPSDARHLDLAVLPKGAGSENWSALRMLNPAEGME
                     GVRRFVLECVVRAGPRPCPPTVVGVGIGGTADQAALLSKRALLRDLGSRNGDGALAAL
                     ETSLLTDLNSLGLGPMGLGGRTTCLDVLIEKASCHTASLPVAVSLSCWALRRAFARVR
                     PDGTVSFRQEGGW"
     gene            2707..3645
                     /locus_tag="PLJ11_05605"
     CDS             2707..3645
                     /locus_tag="PLJ11_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04181.1"
                     /translation="MEAPIQVQGLTKVYGGGFRAVDALDLSVPKRAFMGFLGPNGAGK
                     TTTIKIITNMLAATSGEAYINGVSVRKDPKRALADVGAVVETPEFYPYLTPMETLHYL
                     GSLRGMPASEITERGRDLLELVKLTEWRDQRVGKFSKGMKQRLALAQALLPQPSVIIL
                     DEPTSGLDPRGMVEVRDILRQLHKMDVTVLMSSHLLNEVQEVCTDVALINRGRLLRAG
                     SVADLLNEVKARRIDVRTLRPLAPAMFDRVRAVPGVRGLTITGDLSFAVDFEGEEEAQ
                     AQLLIALQNLGLMVVSFKESGVALESLYMSLIMDSR"
BASE COUNT          611 a         1269 c         1203 g          589 t
ORIGIN      
        1 tggaggagga aagggctgcg ctcctgctgc ctcctcatcc ccttccgggc catgttcctt
       61 gcctcggtca caggggcgag cggccccttg cgggttacct ttcttgatta aggttaaatc
      121 ctcgcttctc ccatgcccta ccaatatgcg caagtcggcc gtcgcgctgc tggccttggc
      181 cctcgtgctc tgctcggcct gcgtccccgg ggggacggag gcggcggggg tcgcaggaac
      241 cgatatagcg ggcagggtgg tgaacgagac cggcgcagcc ttccctggcg tggaggtgcg
      301 cgccgtcaac tcggcctctc gcaccgtcta cagggcgtac agcgggccgg acggcaacta
      361 caccatgcct gatgacttcg tgcaaggaac ctacgccgtc tccgcccact tcaccaacta
      421 ctcggccaac atcacctatt acgatgtgcg ggtcgggctg ggccaggtcc tacgcctgaa
      481 cttcaccatg cgcgaggtcc tgtgcaccct gacaggcttc gtgaccaatg gcaccactcc
      541 cgtctacggg gccacggtga ggctgatcgg cgccgggggc aactactcgg gggtgtccca
      601 gctctcgggg cagtacagca tcaccggggt gaagcccgga acgtacacgg tcgttgccag
      661 caaggtcggc tactatccct cggacccctt ggagccggta gacatgatca ggggcaatac
      721 cacgaacatc gacctcgtcc tgagggagca gcccgccgaa ctcaccggga tggtgaggta
      781 cgacggggac gggctgtcgg gggtcaaggt gcgcctgtcc aacgcccagt tcaccgcgga
      841 gaccatcacg gacgcggagg gcaactacac cttcgcccag gtcccgtcgg gctcttacac
      901 aataacgttc agcaaggacc agttcctcac cgtggagatg agcgtcgggc tgtcgccgtt
      961 cgaggccatg gaactgaaca tcgagatgga gtacgacagc gccaacaaca cccagacatt
     1021 cctgttgggg cttgacctgg cgcattccct catggtcgtc gggctggggg tctccttgat
     1081 aatcttggcc gtgggcctct acatcgacca caaggtccgc cgccgcccgg acgtccttga
     1141 gcgtgaggat gagggggcgg agaaaggctc agagaagggc tgaccgcttg gccacgccct
     1201 cctccacctc cttgaacagg ctccttccat gggcgtccat ggccaccacc atggggccga
     1261 ggccctcggc ccggaggtgc cagaccgcct ccggcatccc caggtcctcc cagtgcacat
     1321 cgatcacctc cgggaaagcc tcggcggcca ccaccgccgc cccgcctgga aaggccaggt
     1381 aggcgcagcc gacctcccgc atcgcctcga ggacctcggc ggacatgccc cccttcccga
     1441 tgatgaggcg gggccggccc aggcggatga gctcgggggc caggcggtcc aggcgggcgc
     1501 tggtggtcgg gccggcggcg atgaccttcc atcccgcgtc ccgccgtacg atcggcccgc
     1561 aatggtagat ggccatgcct tccagccgga acgggaggcc ttccccggcc cgcagcatcc
     1621 gcaccgcgcg caggtgcgcc tcgtcccgga cggtcagcag ctccccgctc agggacacca
     1681 gttccccggc ccgcagcgcc cttgccccct gctccgacag gggcgccttc aactccctga
     1741 gggtcaccat cccccctcct ggcggaagga gaccgttcca tcgggccgga cccgcgcgaa
     1801 cgcccttcgc agggcccagc agctcaggga aaccgccact ggcaggctgg ctgtatggca
     1861 gctcgccttc tctatgagca catcgaggca ggtcgtcctt ccccccagcc ccatcgggcc
     1921 caggcccagg gagttgaggt ccgttaaaag ggaggtctcc agggccgcga gcgcgccgtc
     1981 cccgttccgc gaccccaggt ccctgagcag cgcccgtttt gacagcaatg cggcctggtc
     2041 cgccgtcccc ccgatgccca ccccgaccac ggtcggcggg cagggacggg ggccggccct
     2101 cacgacgcac tcgaggacga agcgccggac gccctccatg ccctcggcgg ggttcagcat
     2161 cctgagggcg ctccagttct ccgagcccgc ccccttgggc agcacggcga ggtccaggtg
     2221 cctggcgtcg gagggctcga agtgcaccgg cggcatcccg gggcccagat tgtcgccgtg
     2281 gttggccctg gagaccgggt ccacggtgtt ggggcgcagc ggcacctcct ccgtcgcccg
     2341 cgccaccccc tggcggatcg ccctctccaa tctcgggtcg tatctgccgc gcacgaagag
     2401 caccggcagg ccggtgtcct ggcacatggg gaccgtcagc tcggccgcca tggccgcatt
     2461 gcgcaggacc acctcgagct gggagcgggg cagcatttcc ggctcgttct ccagggcggc
     2521 gctcagggcg gcgcgcacgt cggccggcag catgcactcc gcctccctca gcagccggac
     2581 caccgcctcc tccacgatcg cggacgaata catctcatcg cctggattga tggaggggga
     2641 gttaatcctt ggcccggtcc cgtggttaat atattatagc accttatgaa tcaatgcccg
     2701 attccgatgg aagcgcccat tcaggtccaa ggcctcacga aggtctacgg gggagggttc
     2761 agggcggtgg acgcgctcga cctgagcgtg cccaagcgcg ccttcatggg cttcctgggc
     2821 cccaacgggg ccgggaagac gaccaccata aagatcatca ccaacatgct cgccgctacc
     2881 tccggggagg cgtacatcaa cggggtgagc gtcaggaagg accccaagcg ggcgctggcg
     2941 gacgtggggg cggtcgtcga gaccccggag ttctacccct acctcacccc catggagacg
     3001 ctgcactacc tgggctcctt gcgggggatg cccgcctccg agatcacgga gcggggccgg
     3061 gacctgctgg agctggtcaa gctcacggaa tggagggacc agcgggtggg caagttctcc
     3121 aagggcatga agcagcgcct cgcgctcgcc caggccctgc tgccccagcc gtccgtgatc
     3181 atcctggacg agccgacctc cggcctggac ccccggggca tggtggaggt gcgggacatc
     3241 ctgcggcaac tgcataagat ggacgtcacc gtgctgatgt cctcgcacct gctgaacgag
     3301 gtccaggagg tgtgcactga cgtcgccctc atcaaccggg gcaggctgct gcgggcgggg
     3361 agcgtcgccg accttctcaa cgaggtcaag gcgcgcagga ttgacgtccg cacgctgcgg
     3421 ccgctggccc cggccatgtt cgaccgggtg cgcgccgtgc cgggggtgag ggggcttacc
     3481 ataacagggg acctctcctt cgccgtcgat ttcgaggggg aggaggaggc gcaggcccag
     3541 ctgctcatcg cgctgcagaa cctcggcctc atggtcgtct cgttcaagga gtcgggcgtg
     3601 gccctggaat cgctgtacat gtcgctgata atggactcga ggtgagatga tggaaggaga
     3661 gaccgtaaac aa
//
LOCUS       DAOSYN010000127         3665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_20301_length_3665_cov_3.599723, whole genome shotgun sequence.
ACCESSION   DAOSYN010000127 DAOSYN010000000
VERSION     DAOSYN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20301_length_3665_cov_3.599723"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..453
                     /locus_tag="PLJ11_05610"
     CDS             76..453
                     /locus_tag="PLJ11_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HOO04182.1"
                     /translation="MLKVRGIDRALMRMVNEAARDSLPNEFAAMLREEDGVVQELVLL
                     PGTLQGKDSATLMLHMMPIDYSIVGTVHSHPGYSNRPSRQDLELFRRSGYVHVITCMP
                     FDEGSWRAYDGAGRPLRLPVVDL"
     gene            complement(450..1676)
                     /locus_tag="PLJ11_05615"
     CDS             complement(450..1676)
                     /locus_tag="PLJ11_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOO04183.1"
                     /translation="MKALILAAGEGTRLRPLTSNIPKPLLTVAGRPYLAHLVEALRDA
                     GVKEQCLLVGWKSNRVKEFFGDGRQMGVELSYLEQKERLGTANAIGVAEGAIDGDFIC
                     VNGDVVMAPEALSQLISAFERDRTPFMGAVEVPDPERFGVVEHVNGKLVRIIEKPADP
                     PSRLINGGVFGLSQEVFGYIRRTGRSPRGEYEFTDTLNMMAAKTDVLVRRIEGEWMDV
                     GYPWELLRANEFLMGRLKRDVRGTVEEGAVLKGEVVVEEGALVRSGSYIEGPVHIAKG
                     CDIGPNCYIRPSTCLGEGVRVGSAVEVKNSIIMKGTHVPHHNYVGDSIIGERCNLGAG
                     TKVANLRFDDRPVKVTAKGQLMGTGRRKLGVIMGDDVRTGINAMIEPGTVIWEGTVVG
                     MGARARGSIGPCSKVF"
     gene            complement(1723..3048)
                     /gene="glmM"
                     /locus_tag="PLJ11_05620"
     CDS             complement(1723..3048)
                     /gene="glmM"
                     /locus_tag="PLJ11_05620"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOO04184.1"
                     /translation="MPGQGCPGDNMGLFGSSGIRGLIGKDIDAEMGVRIGRAVGSLSG
                     TVVVGWDPRTSGDMLVSALVAGMIEAGAEPYRAGMVPTPTLARAASHFDCGVMVTASH
                     NPPEYNGVKLWNPDGSAFSMEQATEVERLIAAGGRRAEWDAMHPERPWEGAIDEHIET
                     IADEVGQLERSVALDCGCGATGVVSPRLLRVLGCRVATLNANPDGRFPGRPSEPAEGN
                     LGDLMALCCARGTVGIAHDGDGDRMVAVDERGRYVSGDRLLALFATLLGVEGMAAPVD
                     ASMVLDDIIGRPVLRTKVGDAFVSEALKRTGLPFGGEPSGTFIFPSVSYCPDGILAGA
                     YLLKLLEGRSLADMVDALPSYPSARDSFPFDPSRREEVAARLYEEISAIEGDLSTVDG
                     YRMDLGYGWLLVRLSGTEPKVRLTVEARTKEDLGRTVELARSKVLKALD"
     gene            3273..3497
                     /locus_tag="PLJ11_05625"
     CDS             3273..3497
                     /locus_tag="PLJ11_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04185.1"
                     /translation="MVMEKISLDKAKDIAKKKGLKPGRVRGTNGIQFTKGNNARLEII
                     SWEEFEKTLNKRNLAVFESGGWMKIMKKTK"
BASE COUNT          640 a         1302 c         1061 g          662 t
ORIGIN      
        1 cccggacagg gcacggttcg tcagcttcgg gaaggggttc tacgctcgta ccagggaaaa
       61 gctgatgggg accctatgct gaaggtcagg ggtatcgacc gggccctcat gaggatggtg
      121 aacgaggccg cccgggactc cctccccaac gagttcgcgg ccatgctccg ggaggaggat
      181 ggggtggtcc aggagctggt cctccttccg gggacgctgc aggggaagga ctcggccacc
      241 ctgatgctgc acatgatgcc catcgactac tccatcgtcg ggacggtgca ctcccacccc
      301 gggtacagca accgcccctc ccgacaggac ctggagctct tccgccgctc cggctacgtg
      361 catgtcataa catgcatgcc cttcgacgag ggcagctgga gggcctacga cggcgccggc
      421 cgtccgctgc gccttccggt ggtcgacctc tagaacacct tgctacaggg gccgatgctc
      481 ccgcgcgccc tggcccccat ccccacgacc gtcccttccc agatcaccgt gccgggctcg
      541 atcatcgcgt tgatgcccgt ccgcacgtca tcgcccatga tcacccctag cttgcgccgg
      601 cctgtgccca tcaactgccc cttggctgtc accttcaccg ggcggtcgtc gaaccgaagg
      661 ttggccacct tggtccccgc cccgagattg cacctttccc cgatgatgct gtcccccaca
      721 tagttgtgat ggggaacgtg cgtccccttc atgattatgg agttcttgac ctccaccgcg
      781 ctgccgacgc gcaccccctc cccgaggcat gtggacgggc ggatgtagca gttcgggccg
      841 atgtcgcagc ccttggctat gtggacgggc ccctcgatgt agctgccgct tcggaccagg
      901 gcgccctcct ccacgaccac ttcccccttg agcaccgcgc cctcctccac cgtccccctc
      961 acatcccgtt tgagccgccc catcaggaac tcgttggccc ggagcagctc ccacggatag
     1021 ccgacgtcca tccattcccc ctcgatgcgc cggaccagca cgtcggtctt ggccgccatc
     1081 atgttgaggg tgtccgtgaa ctcgtactcc cctcggggcg agcggccggt cctgcggatg
     1141 tacccgaata cctcctgaga caggccgaac actcccccgt tgatcagcct cgacggcgga
     1201 tcggccggct tctcgattat ccggacgagc ttgccattga catgctccac caccccgaac
     1261 ctctcggggt ccggcacctc caccgccccc atgaaggggg tgcggtcccg ctcgaaggcg
     1321 gagatcaact gggagagcgc ctcgggggcc atgacaacgt ccccgttgac gcagatgaag
     1381 tccccgtcga tggccccctc ggccaccccg atggcgttgg ccgtccccag ccgctccttc
     1441 tgctccagat agctgagctc cacccccatc tgcctgccgt ccccgaagaa ctccttcacc
     1501 cggttgctct tccagcccac caataggcat tgttccttga ccccggcgtc cctaagggcc
     1561 tccaccagat gcgccaggta gggcctcccg gccacggtca ggagcggctt cgggatgttg
     1621 ctggtcagcg gtctgaggcg ggtgccttcc ccggcggcga ggatgagcgc tttcatcacg
     1681 gtcccttcgg tcgcaaatgg gcgtcagcta ttaaatccct gctcagtcca aggccttcag
     1741 caccttcgac ctggccagct ccaccgtcct cccaaggtcc tccttggtgc gcgcctcgac
     1801 ggtcaaccgg accttcggct ccgttcccga gagcctgaca agcagccagc cataccccag
     1861 gtccatcctg tacccatcga cggtgctcag gtccccttcg atcgccgaga tctcctcata
     1921 gagcctggcg gccacctcct ccctgcggga agggtcgaag gggaaggaat ccctggccga
     1981 gggatagctc ggaagggcgt ccaccatgtc cgccagcgac ctcccctcca gcagcttgag
     2041 gagatacgcc ccggccagga tgccatccgg gcaatagctc accgagggga agatgaacgt
     2101 tcccgagggt tcgcccccga agggaagtcc cgttctcttc aacgcctccg agacgaacgc
     2161 gtcgcccacc ttggtgcgga ggaccggccg gccaatgatg tcgtcaagca ccatcgaggc
     2221 gtccaccggg gcggccatgc cctcgacccc gagcaaggtg gcgaagaggg cgagcaggcg
     2281 gtccccgctg acgtaccgcc ccctctcgtc caccgccacc atgcggtcgc cgtccccgtc
     2341 atgggcgatg cccaccgtcc ccctggcaca gcagagggcc atcaggtccc ccaggtttcc
     2401 ctcggccggc tcggaggggc ggccagggaa cctgccgtcg gggttggcgt tcagtgttgc
     2461 aacgcggcag cccaggaccc ggaggaggcg cgggctgacg accccggtgg ccccgcaacc
     2521 gcagtccaag gccacggaac gctccagctg cccgacctcg tctgcgatgg tctcgatgtg
     2581 ctcatcgatc gccccctccc aggggcgctc tgggtgcatg gcgtcccatt ccgcccgccg
     2641 tccgcctgca gcgatcaggc gctccacctc cgtggcctgt tccatgctga aggcgctccc
     2701 gtccgggttc cacaacttga cgccattgta ttccggcggg ttgtgcgagg cggtgaccat
     2761 gaccccgcag tcgaaatggg aggcggcgcg ggccagggtg ggcgtgggga ccatgccggc
     2821 ccgatagggc tcagctccgg cctcgatcat cccggcgacg agggcgctca ccaacatgtc
     2881 ccccgacgtg cgcgggtccc atcccaccac cacggtgccg ctcaggctcc cgaccgcccg
     2941 tccgatgcgc acgcccatct cagcatcgat gtccttccct atcaacccgc gaatcccgga
     3001 ggaaccgaag aggcccatgt tatccccagg gcatccctgc ccgggcattt aatccctccc
     3061 ccaccagcat tggccagttg ttcagccagc atctcggagc gatccccacc gcgaggctga
     3121 ttatctggat gcgaaatgat gttaacggtc taaatatata agtgaaaatg gctctggtca
     3181 atgtccttta tgcgataaat tttatatata gaatatttcc attacctact ggataacggg
     3241 ccagattggt cgattatcgc ggaggtcatg ccatggtgat ggagaagatc agtttagaca
     3301 aggccaagga catcgcgaag aagaagggtc tcaagccggg tcgcgtcagg ggcacgaacg
     3361 ggattcagtt cacgaagggc aacaacgcaa ggctcgagat aatctcttgg gaggagttcg
     3421 agaagaccct gaacaagagg aacctcgcgg tgttcgagag cggcggctgg atgaagataa
     3481 tgaagaagac caagtaaggg cgtcttgcac cgctcgacca aaccccttaa tgtccctttt
     3541 accctctcct attcttccac tctgtccagg acgaccacca gctcccggcc gaacgccttt
     3601 ttgaaggcct tgcggtcctc ctccatgcgc cacatctcca gctgggggtc cccgctggcg
     3661 gtcac
//
LOCUS       DAOSYN010000128         3657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_20359_length_3657_cov_3.774570, whole genome shotgun sequence.
ACCESSION   DAOSYN010000128 DAOSYN010000000
VERSION     DAOSYN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20359_length_3657_cov_3.774570"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..788
                     /locus_tag="PLJ11_05630"
     CDS             153..788
                     /locus_tag="PLJ11_05630"
                     /inference="COORDINATES: protein motif:HMM:NF016831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO04186.1"
                     /translation="MIEATLEVSIPDGWVHTISVDFDVQIRILDCIPLGDRGARSFVE
                     MECADPAMQQIVSRAIGAHPDVRRWEPALTDDGKLRGIALLTECRACRAIMRSDCHLR
                     SARTVGGGLVEWRIIAAHETALRFLLNELREGGCTTRLLSKKKMDDSSFVTKRQEAAV
                     RSALEKGYYDYPRRVTLQELAKGFGVTASTMGEVLQRGERNIIREYFRTRM"
     gene            799..1638
                     /locus_tag="PLJ11_05635"
     CDS             799..1638
                     /locus_tag="PLJ11_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOO04187.1"
                     /translation="MEDSDRIDVKTMDEKDYRLKRSMARIRHKIMVMSGKGGVGKTTV
                     AVNMAVALAMRGYEVGILDADIHGPNVPKMLNIEHGDVMADEDGLMPVLVPPHLKVMS
                     MAFLLQDPDTPVVWRGPIKMGALRQFLAEVKWGDLDFMIVDLPPGTGDEPLTIAQLIE
                     DADGAIIVTTPQDVALLDSRKSVTFAQALKLPVLGIVENMSGLVCPHCHGVIDLFKVG
                     GGEKAAEEMGVPFLGRVPLDPEVVLGGDDGTPIVLKERENPASKAFEEIVERVLVKVN
                     GGK"
     gene            1639..1968
                     /locus_tag="PLJ11_05640"
     CDS             1639..1968
                     /locus_tag="PLJ11_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO04188.1"
                     /translation="MVRLLVTAEGPTLDDLVEEDFGHARYLLIVDPETWTVEVHDNPK
                     KYTDMGHGIQTAENVAKLRPDAVLTGYAGEHGRRKMEELGIRIITDEEGTVRDAVNGY
                     IRRHMRK"
     gene            2549..3337
                     /locus_tag="PLJ11_05645"
     CDS             2549..3337
                     /locus_tag="PLJ11_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO04189.1"
                     /translation="MLGRVLNRTNGNGNVILLATDGSKPALTATKRAVELAKAMGATL
                     HVVCVEEDIMSMPLERKEELLAEEALLGIHAKGGEVAMEWAARNSVPAVLKTYPGGPV
                     VGAILDYAKQVHPTYIVLGHAGRSGWEKIALGSVAEGVMRHSPYPVIMTRGYDDTYIA
                     DILEVAKTMLRPAEELAEEVPPIPLEQLHIGRKMRIASGTLLAFLVPYFGLGALNSLA
                     VGTATAKVLGDANVAVIWVLLLFPIGWLTAITFHILSSREEAGP"
     gene            3334..>3657
                     /locus_tag="PLJ11_05650"
     CDS             3334..>3657
                     /locus_tag="PLJ11_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOO04190.1"
                     /translation="MTSVVAMVIVVALIAVSMGVAVFTRRSARTSSEFYVAGQNISWL
                     QNALALTGDYLSAASFLGVAGAIAIFGIDKTWDGLGYFGGYIVLLALLAVPLRKVGKY
                     TASEVM"
BASE COUNT          713 a         1035 c         1273 g          636 t
ORIGIN      
        1 ccatggtcat gcttgcaaag aaggggggct ggagctgccc gttgggggag gagggggtag
       61 cgcagcggat aatagacata tgtcgggatt ggcgaagcaa acgggggtaa ccttttctat
      121 ataccattag atcggcggtc tgaggcaggc tgatgataga ggctacgttg gaggtgagca
      181 ttccagatgg gtgggtgcat accatttccg tagacttcga tgtgcagatc cgcatcctgg
      241 actgtatacc gctcggggac agaggcgcgc gctccttcgt ggagatggaa tgcgccgatc
      301 ccgcgatgca gcagatcgtc tccagggcga tcggcgcgca ccctgacgtc cgcaggtggg
      361 agcctgcctt gaccgacgat gggaagctga ggggcatcgc cctgctcacc gagtgccggg
      421 catgcagggc catcatgcgc tccgactgcc atctgcgctc cgcgcgcacg gtcggtggcg
      481 gacttgtgga atggcggatc atcgccgcgc acgagaccgc cctgcgtttc cttttgaacg
      541 aactgcggga gggcggctgc accaccaggc tgctgtccaa gaagaagatg gacgatagca
      601 gcttcgtcac caagaggcag gaggccgcgg tgcgctccgc cttggagaag ggctactacg
      661 attatccccg gagggtcacg ctgcaggagc tggccaaggg gttcggggtc accgcttcca
      721 ccatggggga ggtcctccag cggggggagc gcaacatcat ccgggagtac ttccggacca
      781 ggatgtgagg aggatagaat ggaagattcg gataggatag acgtgaagac catggacgag
      841 aaggactacc ggctcaagag gtccatggcc aggataaggc acaagatcat ggttatgtcc
      901 gggaagggtg gggtgggcaa gaccaccgtc gcggtgaaca tggccgtcgc cctggcgatg
      961 cgcgggtacg aggtcggcat cctggacgcg gacatccatg gcccgaacgt ccccaagatg
     1021 ctcaacatcg agcacgggga cgtcatggcc gacgaggacg ggctcatgcc cgtcctggtg
     1081 cccccgcacc tcaaggtcat gtccatggcc ttcctgctgc aggaccccga cacccccgtg
     1141 gtgtggaggg ggccgatcaa gatgggcgcc ctccgtcagt tcctggccga agtgaaatgg
     1201 ggcgacctgg actttatgat cgtggacctg cccccgggca caggggatga gccgctgacc
     1261 attgcccagt tgatcgagga cgccgatggg gcgatcattg tcaccacgcc gcaggacgtg
     1321 gccctgctgg actcccgcaa gagcgtgacc ttcgcccagg ccctgaagct gcccgtcctg
     1381 gggatcgtgg agaacatgag cgggctcgtc tgcccgcact gccatggtgt catagacctg
     1441 ttcaaggtcg gcggcgggga gaaagcggcc gaggagatgg gggtgccgtt cctgggccgg
     1501 gtgccattgg accccgaggt ggtgctgggc ggggacgacg ggacgcccat cgtgctcaag
     1561 gagagggaga accccgcctc caaggccttc gaggagatag tggagagggt gctggtcaag
     1621 gtgaacggag ggaagtgagt ggtccggctg ttggtgacgg ccgaaggccc caccctggac
     1681 gacctggtgg aggaggactt cgggcacgcc cgctacctcc tcatcgtcga cccggagacc
     1741 tggacggtgg aggtccatga caaccccaag aagtacaccg acatgggcca tggcatccag
     1801 acggctgaga acgtggccaa gctgaggccg gacgcggtgc tcaccgggta cgcgggggag
     1861 cacggccgaa ggaagatgga ggagctgggg ataaggatca taacggacga ggaggggacc
     1921 gtgcgggacg cggtcaacgg gtacatccgc aggcatatga ggaagtgagg ctccaccgga
     1981 acgtccgctg cctaaaggaa tcgggccgtt acgacgaacg ccgttctgtt cttttcattt
     2041 tattcgtatt tgtacgaagg ggtacgtatg cccccttgct gctggctcgt aaggaatgct
     2101 agtcattggc acgcaatggc catcgactcc gagcgttgca tctccgattt tcaatgcgca
     2161 tatagattat aatcgtaata tttctgcaat ttcagtaatt atttcccaaa atatctgtga
     2221 atatcgcagg ttatatccat agctttaagt aagggtctga aagtagctac cccctcatgg
     2281 atgtcagatc ccatccatct gagagcagca aacggcactg ccccgccggt gccggcctcg
     2341 atcggtcaga gagagaggtt gcctgggcgg acctcctgga cgagcatggc gttgccaatg
     2401 cccggagcag gaggcggttt gaaagcgcca tccgcgaaag gatgagggcc gggggagggc
     2461 gctcgatgag cgccgttaat ccgcgaaggg gctggcctga cccaaggcag cgcctaggcg
     2521 agcgcgcgcc gcgaggaggt gccctctgat gctggggcgg gtactcaacc ggacgaacgg
     2581 caacgggaac gtcatcctcc tggccaccga cggctccaag cctgccctca ccgccaccaa
     2641 gagggcggtg gaactggcca aggccatggg cgccaccctg cacgtcgtct gcgtggagga
     2701 ggacatcatg agcatgcccc tcgagcggaa ggaggagctc ctggccgaag aggcgcttct
     2761 gggcatccat gccaagggag gggaggtggc catggagtgg gccgcccgca actccgtccc
     2821 ggcggtgctc aagacctatc cgggggggcc ggtggtgggg gccatcctgg actatgccaa
     2881 gcaggtccat cctacgtata tagtcctggg gcatgccggg cgcagcggct gggagaagat
     2941 cgccctgggc tcggtggccg agggggtgat gaggcactcc ccctacccgg tgataatgac
     3001 caggggctat gacgacacgt acatagccga catcctggag gtcgccaaga ccatgctgag
     3061 gcccgcggag gagctggccg aggaggtgcc tccgatcccc ctcgagcagc tgcacatagg
     3121 gcgtaagatg aggatcgcct ccggcaccct cctggcattc ctggtgccct acttcgggct
     3181 gggcgcgctc aactcgctgg ccgtgggcac ggccaccgcc aaggtcctgg gggacgccaa
     3241 cgtggcggtg atctgggtgc tgctgctgtt ccccatcggc tggctcaccg cgatcacgtt
     3301 ccatatcctg tcgagcaggg aggaggccgg accatgacca gcgtcgtggc gatggtcatc
     3361 gtggtggccc tcatcgccgt atcgatgggg gtcgccgtgt tcacccggcg ctcggcaagg
     3421 acctcctcgg agttctacgt ggccgggcag aacatcagct ggctgcagaa cgccctcgcg
     3481 ctcaccgggg actacctgag cgcagcgtcc ttccttgggg tggcgggcgc catagccata
     3541 ttcggcatag ataagacctg ggacgggctg ggctacttcg gcggatacat agtgctcctg
     3601 gcgctgctgg ccgtgccgct gaggaaggtg ggcaagtaca ctgcgtcgga ggtgatg
//
LOCUS       DAOSYN010000129         3618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_20625_length_3618_cov_5.211619, whole genome shotgun sequence.
ACCESSION   DAOSYN010000129 DAOSYN010000000
VERSION     DAOSYN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20625_length_3618_cov_5.211619"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="PLJ11_05655"
     CDS             complement(<1..1994)
                     /locus_tag="PLJ11_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HOO04191.1"
                     /translation="MEIEDRYPTKRSLVIPFEQLNSLNDEFAAYVLKHPDETLEIGKT
                     ILRTQTDKDDVGIRITDLPSDSHVEISKLRAEHLSKLISINGLVRKTTAVRPNIKIAR
                     YKCARCSAIISEPQTSNILTEPLECYKEQEGCGRTLSTTKFTLVASECYTTDIQKIEV
                     QENPEGLRGGTQPGRITAYLSHEITGVIQPGMRVTLNGILRMKLDDGRTKSTMTDMEL
                     EVISYKLDEGDLDDNRLTDDDMVRIREMAASPSLMNDLVRSIAPGIKGYDVEKEALLY
                     QQVGGVAKELDDGQKMRGDIHILLVGDPGVAKSQLLHYMCELAPRGIYASGKSSSAAG
                     LTAAAVKDDFGEGRWVLEAGALVLADKGLACIDELDKMNDNDRSALHEAMESQQVTIS
                     KAGINATLKCRFCLLGGANPKFGRFDDLEPIASQIDMPPTLLSRFDLIFAIKDKPDKD
                     KDRGIAEHICKGQMRAGALKHIHDQDEMADNILRTTNDLRPVYTRDEIRKYLIYARSQ
                     APIITPRATDTIVEHYTKIRQLSADGAPTVPITPRQMEAFLRISEASAKLRLSKYVED
                     QDVERAISVVMYYLNTIATNEEGKQDIDVLMTGRTGKERNKLQTIAHILRTHGPIKEE
                     ELIDICRTNGIDEYDVTKAIKQLSEAGQIYEPKFGVYQLMG"
     gene            complement(2241..2918)
                     /locus_tag="PLJ11_05660"
     CDS             complement(2241..2918)
                     /locus_tag="PLJ11_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04192.1"
                     /translation="MKIRARKFKEFLTLAKKQWNEPCLRMNDEGLSTVTVDPAHVSML
                     SAIIKPSDDCIPDDKERMFCIDADKLVRFLLNYSPSDMLNVELSDERMTVTDGKMTFK
                     LALLTPEAWPKFPDLESKLTSGGSFNVRDLKAIYDCHFENLAFKIEDETLIAEANNKN
                     DSIVVKGEIGGKAQAQSIYPMEYVNNALLGEKIYVKFATDLPCLLIPQLENIEARIML
                     APRIEND"
     gene            complement(3360..>3618)
                     /locus_tag="PLJ11_05665"
     CDS             complement(3360..>3618)
                     /locus_tag="PLJ11_05665"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HOO04193.1"
                     /translation="KGPFEGPIYVEVDFWISRPKSLNKKITLPTGRKNDLDKLLRCVL
                     DGLTGICYIDDGQVVYISKATKCYADEEHPPGCNITIGMVQ"
BASE COUNT          773 a          947 c          751 g         1147 t
ORIGIN      
        1 cccatcagtt gatatacccc gaatttaggc tcatatatct gtcctgcttc cgataattgc
       61 tttattgctt tagttacgtc atactcgtct attccgttcg tgcggcatat atctatcagt
      121 tcctcctcct ttattggccc gtgcgtcctc agtatatgcg ctattgtctg aagcttattg
      181 cgctcctttc ccgtcctgcc tgtcatcaag acatcgatat cctgctttcc ctcctcgttg
      241 gtcgcgatcg tgttcagata gtacatcaca actgatatgg cgcgttctac gtcttggtcc
      301 tctacatatt ttgatagccg gagctttgcg gacgcctcgg agatcctcaa gaatgcttcc
      361 atctgtctcg gtgttatcgg gactgtaggt gcgccgtctg cgctcaattg ccttatttta
      421 gtgtaatgtt cgacgatcgt atctgtggcc cttggggtta ttatcggagc ttgggatcgc
      481 gcatagatca agtattttct gatttcatct ctagtatata ccggccttag atcatttgtc
      541 gtccttaata tgttatcagc catctcgtcc tggtcatgga tatgcttgag cgctccggcc
      601 ctcatctgtc ctttgcatat atgctcagcg atcccccggt ccttatcctt atcaggcttg
      661 tccttgattg cgaagatgag atcgaacctg ctgagcaatg tcggcggcat atctatctga
      721 gaggctatcg gctcaagatc atcgaaccgt ccaaacttcg gattggcccc gcctagtaag
      781 cagaacctgc atttcagagt ggcatttatt ccggccttgg aaattgtgac ctgctggctc
      841 tccatagcct catgcagtgc tgagcggtcg ttatcattca tcttgtccaa ttcgtcaatg
      901 cacgccaatc ccttatccgc aaggaccaat gctccggctt ccaataccca tcgcccctcc
      961 ccgaagtcat cctttacagc cgcagccgtc aatcctgcag cgcttgatga tttgccagaa
     1021 gcgtatatcc ccctgggagc aagttcgcac atataatgca gtaattgaga ttttgccacc
     1081 ccaggatctc ctactagaag gatatggata tcgcccctca tcttctgccc atcatccaat
     1141 tccttagcta cacctcctac ttgttgatat aatagtgctt ctttttccac gtcgtatcct
     1201 ttaatgcctg gggcgatcga acgaacaagg tcattcatta atgaaggact ggctgccatt
     1261 tctcttattc ggaccatatc atcatcagta agccgattgt catcaagatc gccctcatcc
     1321 agcttatagg atatgacctc gagctccata tccgtcatgg ttgatttggt ccgcccgtcg
     1381 tctaatttca tccttaatat cccattcagt gttacgcgca tccctggctg gatcactcct
     1441 gttatttcat gggatagata ggctgtgatc cttcctggct gggttccccc tcttagtcct
     1501 tccgggttct cttggacctc gatcttctgg atgtccgtag tatagcattc cgaggcaacc
     1561 agtgtaaact tggttgtgct caatgtcctc ccgcatcctt cctgctcctt atagcattcc
     1621 aagggttcgg tcaatatatt ggatgtctga ggctcgctga tgatcgccga gcatctggcg
     1681 cacttgtatc tggcgatttt gatgttaggt ctgactgcag tggtctttct gaccaggccg
     1741 ttgatgctta tcagtttgct cagatgctcg gctcttaact tggagatttc gacatgggaa
     1801 tcgcttggaa gatcggttat tcgtatccct acatcatcct tatcggtttg tgtcctcagg
     1861 atggtttttc cgatctccaa ggtttcatcg ggatgcttta agacgtaggc cgcgaactcg
     1921 tcattaaggc tattcaattg ctcgaatggg atgaccagtg aacgcttggt aggataacgg
     1981 tcctcgatct ccatcagttg cacatgctca ttatctatta tttggcccca taaggaccgt
     2041 atatcatctt cttgatattc tgccatttat cccatcacca ccgcccattt tccgcaaaat
     2101 cggcattgaa agaccaacat tatattatca gtccctttta tcgggatcgc ggtccttatc
     2161 atcatcggct tattgcattc ggggcatcct ataggattcc ttatcggtag ttcttctttg
     2221 tttttttcca tgtttccacc tcaatcgttc tctatccgag gagccagcat tatccttgct
     2281 tctatgttct cgagctgtgg gatcaatagg cacggaaggt ccgtagcgaa tttcacatat
     2341 atcttttccc cgaggagcgc attattcacg tactccatcg gatatatgga ctgtgcctga
     2401 gcttttcctc ctatttctcc tttgacaact atgctgtcat tcttattgtt tgcttctgcg
     2461 atcaatgttt catcctcgat cttgaatgcc aggttttcaa aatggcaatc atatatggcc
     2521 ttaagatcgc gaacattgaa tgatccgccg gatgttaatt tggattccaa gtccgggaat
     2581 ttcggccatg cttcaggagt caagagcgcc aatttgaacg tcatctttcc gtccgttact
     2641 gtcatccgtt catcgcttaa ttctacattg agcatgtcag atgggctata gttcaacagg
     2701 aacctgacca gcttatcggc gtctatgcag aacatgcgct ccttatcatc ggggatgcaa
     2761 tcatccgatg gcttgatgat cgcagatagc atcgaaacat gggcaggatc tacagttaca
     2821 gtagatagcc cctcgtcatt cattctcaag catggctcgt tccattgctt ttttgcaagc
     2881 gtcaggaact ccttgaattt cctcgctctg attttcatag tttcagctcc gttgtttatt
     2941 tagctctccc atcttttgac caataggcag accgcgaatc cagaccgtgg ataaatgtat
     3001 cccctaaagc atactgggac atatacggtc atctcctcat atggggagat ttcctccctt
     3061 agtccaggat caggaccatt gacgcattcc tggcattgag cgcatcgcat ggttcggata
     3121 gatgcctcag tagtagatgg tcgttgtcct ccagccgtgt aatcaatcaa tccgttcctc
     3181 cccccatagg atcgcggtca tcaattatgg gatcgtcatc aggccctgat atagctatct
     3241 ttggttttct tatttgcggc attggtcaac ccccttcata tatagttcct aaactatcca
     3301 gacgaatagc tctatgatga ctgttattac tacgatcatc caatatagat ttccaagcgt
     3361 cattggacca tccctatggt tatattgcac cctggcggat gttcttcatc agcataacac
     3421 ttggtggctt tactgatata tacgacctgg ccatcgtcaa tatagcatat cccggtcagg
     3481 ccgtcaagca cgcagcgaag caatttatct agatcgttct ttctgccagt cggcaatgtt
     3541 atttttttat tcagcgactt agggcgggat atccagaaat cgacctcgac atatatcgga
     3601 ccttcaaacg gtcctttt
//
LOCUS       DAOSYN010000130         3613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_20651_length_3613_cov_4.027263, whole genome shotgun sequence.
ACCESSION   DAOSYN010000130 DAOSYN010000000
VERSION     DAOSYN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20651_length_3613_cov_4.027263"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /gene="phoU"
                     /locus_tag="PLJ11_05670"
     CDS             complement(<1..615)
                     /gene="phoU"
                     /locus_tag="PLJ11_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOO04194.1"
                     /translation="MAPRTTFNYEMEGLQKEITEMANASRAAFEKAVRMITDGDLSLR
                     EEVRALDRHVYELNERIDKHCLDIIALHSPVASDLRLISACLKMVEDLNRFGRYATDI
                     AELIDDFEEGKAFKRFESLRSMSCLVVGMVDDAVYSFLQRDAEKASHLHERDDAVDSL
                     YDTIFREILTYMMEDPRKITVGIDLILVARYMERVADHACNIGEY"
     gene            complement(615..1406)
                     /gene="pstB"
                     /locus_tag="PLJ11_05675"
     CDS             complement(615..1406)
                     /gene="pstB"
                     /locus_tag="PLJ11_05675"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOO04195.1"
                     /translation="MTIAIRTDDLHVYFDKNHALKGVSMDIEMNQVTAVIGPSGCGKS
                     TFIRCINRMNDLIPGCTVKGKVLVDGMDINDDDVDVVDVRRRIGMVFQKPNPFPKSIY
                     ENVAYGPRLHGVVETGNGKGRRRKHTKRELDEIVEHSLRRAALWDEVKDRMDKSGTSL
                     SGGQQQRLCIARTLAVDPEIILFDEPCSALDPIATAKIEDLLVDLKKDYTVVIVTHNM
                     QQAARISDRTAFFYLGDLIEMGDTKQIFERPQKELTEQYITGRMG"
     gene            complement(1408..2265)
                     /gene="pstA"
                     /locus_tag="PLJ11_05680"
     CDS             complement(1408..2265)
                     /gene="pstA"
                     /locus_tag="PLJ11_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOO04196.1"
                     /translation="MMLNRRATEMAVFTLFRGCALLVVLALVVLLGYIVSGGLERLSV
                     EFLTAMPRRANTEGGIFPAIVGTFYLMLIVLSISVPIGVMSAIYMVEYEQNSLMGKVF
                     KLAVYNLAGVPSIVYGLLGLGIFVAFLGFGFSLISAGLTLSIMTLPLMISSAREAIAA
                     VPSTVREASYALGATKWQTIWHHVLPYALPGIFTGMILSVSRAAGETAPLMLTGVAMS
                     LQRLPDSFFDSFMALPYHVFYMATQSSDLLATRPIQYATALVLLLLVLGMNLFAIVLR
                     KRYRDRYRW"
     gene            complement(2265..3155)
                     /gene="pstC"
                     /locus_tag="PLJ11_05685"
     CDS             complement(2265..3155)
                     /gene="pstC"
                     /locus_tag="PLJ11_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOO04197.1"
                     /translation="MRKNRHLPPREKAIQLALTVATWSSVLALAAVFGELLLGALPAL
                     SSISIIDFFTGTVWNPSHPIYPLYGILPLFVGTMMVSFGAALIAIPVGLGCSIWLAEL
                     ASPRVKAIFKPAIEILAGIPSVIFGFFALVILSEWIAVVFEPVTKLNALNGAIMLAVM
                     MIPIMVTVAEDAMNAVPMSLREASLALGATRWETVRHVVIPASLSGVVAAVVLAFGRA
                     VGETMTVLMATGNATALHFDILRSVQTMTAAIAIDFGEVEFGSEHYHVLFLVGLVLFV
                     MTFLINFLADWVTRRFKEDY"
     gene            complement(3267..>3613)
                     /locus_tag="PLJ11_05690"
     CDS             complement(3267..>3613)
                     /locus_tag="PLJ11_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04198.1"
                     /translation="LSGNSAIVSAVQGDEGGIGYVGIGYASASGINVLELKKNSTSEA
                     YSPLDAEAVQSGTYDLSRYLYIYTDGTPTDQISRWLSWILNADLGQKVAEEIGFYALS
                     AEVIAEERAKLG"
BASE COUNT          675 a         1125 c         1097 g          716 t
ORIGIN      
        1 gtattccccg atgttgcatg cgtggtctgc cacccgctcc atgtacctgg cgaccaatat
       61 gaggtctatg cccaccgtga tcttcctggg gtcctccatc atgtacgtca gtatctcccg
      121 gaatatggtg tcgtacagcg agtcgacggc gtcgtccctc tcatgcaggt gggacgcctt
      181 ctcggcgtcc ctttgcagga acgaatatac ggcatcgtcc accatcccga ccaccaggca
      241 ggacatgcta cggagcgact cgaagcgctt gaaggccttg ccctcctcga agtcgtctat
      301 cagctccgcg atgtccgtgg cgtaccggcc gaagcggttg aggtcctcga ccatcttcag
      361 gcacgccgag atcaaacgca ggtcgctggc gaccggcgaa tgcagggcga ttatgtccag
      421 gcagtgcttg tcgatccgtt cgttcagctc atagacgtgc ctgtccaggg cgcgcacctc
      481 ctcgcgcagg gagaggtccc cgtcggtgat catccgcacc gccttctcga aggcggcccg
      541 agaggcgttg gccatctccg ttatctcctt ctgcagcccc tccatctcgt aattgaacgt
      601 ggttcgtggc gccatcagcc catcctcccg gttatgtact gctccgtgag ctccttctgc
      661 ggtctttcga atatctgctt ggtatcgccc atctctatca ggtcgcccag gtagaagaag
      721 gcggtcctgt cggatatcct cgccgcctgc tgcatgttgt gcgtgacgat gacgacggtg
      781 taatccttct tcaggtccac cagcaggtcc tctatcttgg ccgtggctat ggggtccagg
      841 gcggagcagg gctcgtcgaa gaggattatt tccggatcga ccgcgagcgt ccgcgcgatg
      901 cacagcctct gctgctgtcc ccccgatagg gacgtcccgc tcttgtccat gcggtccttg
      961 acctcgtccc agagggccgc cctgcgcagg gagtgctcga cgatctcgtc cagttccctc
     1021 ttggtgtgct tgcgtctgcg ccccttgccg ttccccgttt ccacaacgcc gtgcaaacgc
     1081 ggcccgtagg ccacgttctc gtagatgctc ttcgggaagg ggttcggctt ctggaacacc
     1141 attcctatgc ggcgacggac gtccaccacg tccacgtcgt catcgttgat gtccatgccg
     1201 tccaccagca cctttccctt cacggtgcag ccggggatca ggtcgttcat gcggttgatg
     1261 cacctgatga acgtggactt gccgcagccg gacgggccga tcacggcggt cacctggttc
     1321 atctcgatgt ccatgctcac gcctttgagg gcgtggttct tgtcgaagta cacgtgaagg
     1381 tcatccgtgc ggatcgctat ggtcatttta ccacctgtac ctgtcgcggt atcttttcct
     1441 gagcacgatg gcgaagaggt tcatccccaa taccaatagc aggagcacca gggccgttgc
     1501 gtactggatc gggcgggtgg ccagcaggtc ggacgactgc gtggccatat agaatacgtg
     1561 atagggcagg gccatgaagc tgtcgaagaa gctgtccggc aacctctgga gcgacatcgc
     1621 gacgccggtc agcatcagcg gcgcggtctc cccggccgct ctggacacgg agaggatcat
     1681 gccggtgaag atgccgggga gcgcgtacgg aagaacgtga tgccagatcg tctgccattt
     1741 ggtggcgccg agagcgtatg acgcttcccg aaccgtgctt ggcacagcgg ctatcgcttc
     1801 cctggccgac gatatcatca gcggcagggt cattatgctc aaggtgaggc cggccgagat
     1861 cagggagaac ccgaagccta ggaaggcgac gaatatcccc aggcccagca gtccgtagac
     1921 tatggaaggc actccggcca gattgtatac ggccagcttg aacacctttc ccatcaagga
     1981 gttctgttca tactccacca tgtagatggc ggacataacg ccgattggga ccgatatcga
     2041 cagcacgatc agcatcaggt agaacgtgcc cacgatggcc gggaatatgc cgccctcggt
     2101 gttcgcccgg cggggcatcg ccgtgaggaa ctcgacgctg agccgctcca gtccgccgga
     2161 gacgatatat ccgagcagca ccaccaacgc cagcacgacc agcagggcgc agccccggaa
     2221 cagggtgaag accgccattt cggtggccct gcggttcagc atcatcagta gtcctccttg
     2281 aagcgccggg tgacccaatc ggccaggaag ttgatgagga aggtcatcac gaacagcacc
     2341 agcccgacga ggaagagcac gtgatagtgc tcagaaccga actccacctc gccgaagtcg
     2401 atggcgatgg ccgccgtcat cgtctgcact gagcgcagga tgtcgaagtg cagcgcggtg
     2461 gcgttcccgg tggccatgag cacggtcatc gtctccccga ccgcccggcc gaaggcgagc
     2521 acgacggcgg cgaccacccc cgacagcgag gcggggatga cgacatggcg gacggtctcc
     2581 catctcgtcg cgcccagggc cagggatgcc tccctcaggc tcatgggaac ggcgttcatg
     2641 gcatcttcgg ccacggtgac cattatgggt atcatcatca ccgccagcat tatcgccccg
     2701 ttcagggcgt tcaatttcgt caccggctca aagaccacgg cgatccattc cgacaggatc
     2761 accagggcga agaagccgaa gatcacggac ggaatgccgg ccaagatctc gatggccggc
     2821 ttgaatatgg ccttcacccg ggggctggcg agctcggcca gccagatcga gcaccccagt
     2881 ccgacgggga tggcgatgag cgcggcgccg aaggacacca tcatggtgcc tacgaacagg
     2941 gggagtatgc cgtagagagg gtaaatggga tgggaagggt tccacaccgt cccggtgaag
     3001 aaatcaatta tactgatgga ggagagcgcc ggcagcgccc ccagaagcag ctcgccgaac
     3061 acggccgcca gcgccaggac ggaggaccag gtggccactg tcagcgccag ctggatggcc
     3121 ttctcccggg ggggcaggtg cctgttcttg cgcaacctcc ggcgccctcc ttcctttctc
     3181 acccttgatg ccactgttcc ttcgctcctt ttagggtttt gaaggggttt gatagggttt
     3241 ggtaaaaggt gggtgatggg tttggatcag cccagcttag ccctctcctc tgcgatgacc
     3301 tcggccgata gggcgtagaa acctatctcc tcggccacct tctggcccag gtccgcgttc
     3361 aggatccagc tcagccagcg gctgatctgg tccgtgggcg tcccatcagt atagatgtat
     3421 aggtacctgg acaggtcgta cgttccggac tggacggcct cggcgtccag cggcgagtag
     3481 gcctcggacg ttgagttctt cttcagctcc aggacgttga tgccggaggc gctggcgtaa
     3541 ccgatcccca cgtagcctat gccgccctcg tcgccctgca ccgccgagac gatggcggag
     3601 ttgccggaga gca
//
LOCUS       DAOSYN010000131         3600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_20738_length_3600_cov_3.667701, whole genome shotgun sequence.
ACCESSION   DAOSYN010000131 DAOSYN010000000
VERSION     DAOSYN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20738_length_3600_cov_3.667701"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="amrB"
                     /locus_tag="PLJ11_05695"
     CDS             complement(<1..601)
                     /gene="amrB"
                     /locus_tag="PLJ11_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOO04199.1"
                     /translation="MVRRPAVAGQFYSSSRSALMKELEQCFLSPLGPGKVPTVAKAGP
                     RRLVGAMVPHAGLVFSGPVAAHAYAEMAADGMPETAVIIGPNHTGYGRAVSASSEDFE
                     TPLGVVRADRDIVSRLRGVVEIDCTAHLYEHSLEVQLPFLQYLCPDIKIVPIVMGRQD
                     MVMAKETAKVLREACAGKDVVFLASSDLSHYVPPEVARER"
     gene            complement(641..1252)
                     /gene="rpsB"
                     /locus_tag="PLJ11_05700"
     CDS             complement(641..1252)
                     /gene="rpsB"
                     /locus_tag="PLJ11_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOO04200.1"
                     /translation="MSDETKTELLVPEDVYLTSGVHIGTQQKSADMKDFIFKVRTDGL
                     YVMDVKQTDVRIRAAAKFLSRFKPERILVVSARQYGQKPVRVMSKAIGAMYFEERLIP
                     GSLTNPMLDRYIEPDVIVVTDPAADQQAVAEALNVGIPIVALCDANNETRNVDLVIPT
                     NNKGRRSLACVYWLLTREVLKEQGKLARDEDFKLTIDDFEASL"
     gene            complement(1263..1436)
                     /locus_tag="PLJ11_05705"
     CDS             complement(1263..1436)
                     /locus_tag="PLJ11_05705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HOO04201.1"
                     /translation="MKYTRFEKARIIGARALQISLGAPVLLDIPEGMIDPIAIAQLEF
                     DKGVIPITVKRDI"
     gene            complement(1608..2090)
                     /locus_tag="PLJ11_05710"
     CDS             complement(1608..2090)
                     /locus_tag="PLJ11_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOO04202.1"
                     /translation="MRGRVWKYGDHVNTDVIIPGRYLDDYDVRSMAKHALEDLDPRFA
                     KDVAYGDVIVAGLNFGCGSSREQAPLALKHAGVGAVVAISFARIFFRNAVNVGLPVVE
                     LDATGMFERGDVALINLEKGVIVNETTGRTGRFEPLPDFLLDILRDGGLVPHTAKRLH
                     "
     gene            complement(2087..3346)
                     /locus_tag="PLJ11_05715"
     CDS             complement(2087..3346)
                     /locus_tag="PLJ11_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOO04203.1"
                     /translation="MEAMTFAQKVLSRASGREAFPGEIVSAKIDLAMSHENAALVLRA
                     FNEMGAKEVWDAERMVLLFDHRVPANTIKAAEGHQAVRAFVRTEGLPHFYDLREGICH
                     QVLPEKGHVLPGMLIVGTDSHTTTYGALGALSTGIGATEMAAVWATGELWMKVPRTFR
                     FHLTGRLDGPVVAKDVILHLIGMMGADGCDYRCAEFVGPVVDAMSISGRMTMCNMSME
                     MGAKAGVAFPDQRTRDYLKGRTAKEWKPVLSDPEAQVEREIALDLTGLAPQVAYPHSV
                     DNVKPVEEMAGIPVDQAVIGSCTNGRAEDLLAAERVLHGRKVPDSVRLIVVPASREVY
                     LWALESGVMASLVRAGAVVTNPGCGPCLGAHQGLLAPGERCIATTNRNFRGRMGSPES
                     EVYLASPATVAASALKGQIADPREVIG"
BASE COUNT          614 a         1181 c         1095 g          710 t
ORIGIN      
        1 ccctctcccg ggcgacctcc gggggaacgt aatgggaaag gtcggaggag gccaggaaga
       61 ccacgtcctt cccggcgcat gcctcccgca gcaccttggc ggtctccttg gccatgacca
      121 tgtcctggcg ccccatgacg atgggcacga tcttgatgtc cgggcagagg tactggagga
      181 aaggcaactg cacctccagg ctgtgctcgt agagatgggc ggtgcagtcg atctccacca
      241 cccccctcag cctggacacg atgtcccggt ccgccctcac cacgcccaga ggcgtctcga
      301 agtcctcgct gctggccgac accgccctgc cgtacccggt atggttcggc ccgatgatca
      361 cggccgtctc cggcatgcca tcggcggcca tctccgcgta tgcgtgcgcc gccacaggcc
      421 cggagaacac caggcccgca tggggcacca tcgccccgac cagccgtctg gggccggcct
      481 tggcgaccgt ggggaccttc cccgggccca ggggggagag gaaacattgc tccagctcct
      541 tcatcagagc gctcctggac gaggaataga actggccggc gacggcgggg cggcgcacca
      601 tgtatgaaga taggaaaagg gggttgaaaa gggttggggt ttaaaggctg gcctcgaagt
      661 cgtcgatggt cagcttgaag tcctcgtccc gggcgagctt gccctgctcc ttgagcacct
      721 cgcgggtcag aagccagtag acgcatgcca gggagcgcct tcccttgttg ttggtcggga
      781 tgacgagatc gacgttcctg gtctcgttgt tggcgtcgca gagggccacg atcgggatcc
      841 ccacgttcag cgcctcggcc accgcctgtt ggtcggcggc ggggtcggtg accactatga
      901 cgtccggctc gatgtagcgg tcgagcatgg ggttggtgag ggagccgggg atgagccgct
      961 cctcgaagta catggccccg atggccttgg acatgaccct caccggcttt tggccatact
     1021 gcctcgcgga cacgaccaat atcctttccg gcttgaagcg ggagaggaac ttggcggccg
     1081 ccctgatgcg gacgtcggtc tgctttacgt ccatgacgta caggccatcg gtcctgacct
     1141 tgaagatgaa atccttcata tcagcactct tttgctgggt gccaatgtgg accccggagg
     1201 tcagatagac gtcctccggt acgagaagct cggtcttggt ctcgtcgctc atttcaataa
     1261 cctcatatgt cgcgctttac cgttattggg ataactccct tgtcgaactc caattgagca
     1321 atcgcgatcg ggtcgatcat cccttcgggg atatctagaa gcacaggcgc gcctagggat
     1381 atctggagtg ccctggcacc tattatgcgc gccttctcaa atctagtata cttcatgaat
     1441 tcaccataag ggggagtgtc cccatattag cggggggctt ataaattttt tcacgacctg
     1501 catgggacat gccagatgcc tgttttgccc gttccctcgc ctatttgcgg gggtgtcatc
     1561 ggccatcctc ccaccctgcc aacccttgcc tgtataaata agttgcctca atggagcctc
     1621 ttggcggtat ggggcaccag gccgccgtcc ctcaggatgt ccaataggaa atcgggcaga
     1681 ggctcgaacc tcccggtgcg gccggtcgtc tcgttcacga tgaccccctt ctccaggttg
     1741 atcagggcca cgtcccccct ctcgaacatt ccagtggcgt caagctccac cacgggcagg
     1801 ccgacgttca ccgcgttgcg gaagaagatg cgggcgaagc tgatggcgac gacggccccg
     1861 accccggcgt gcttgagcgc caggggcgcc tgctccctgc tcgagccgca gccgaagttc
     1921 aggccggcga cgatcacgtc cccgtaggcg acatccttgg cgaatctggg gtccaggtcc
     1981 tcgagggcat gtttggccat gcttcggaca tcgtagtcgt ccaggtacct tccggggatg
     2041 atcacgtcgg tgttcacgtg gtccccgtac ttccacaccc tgcccctcac ccgatcacct
     2101 cccgggggtc ggcgatctgc cccttcagcg cggaggcggc gaccgtggcg ggcgaggcca
     2161 ggtacacctc cgactctggc gagcccatgc gcccgcggaa gttgcggttg gtggtggcga
     2221 tgcacctctc cccgggggcc agcagcccct ggtgcgcgcc gaggcatggc ccacaccccg
     2281 gattggtcac cacggccccg gcgcggacca aagaggccat gacgccactt tccaacgccc
     2341 agaggtacac ctcgcgcgag gctggaacga cgatgaggcg cacgctgtcc gggaccttgc
     2401 gcccgtgcag gaccctctcc gcggccagga ggtcctccgc ccgcccgttg gtgcaggagc
     2461 cgatgacagc ctggtccact ggaatccctg ccatctcctc caccggtttg acgttgtcca
     2521 ccgaatgggg ataggcgacc tgcggcgcaa ggccggtcag gtcgagggcg atctcccttt
     2581 ccacctgggc ctccgggtcg gagagcactg gcttccattc ctttgccgtc cttcccttca
     2641 gatagtccct ggtcctctgg tccgggaagg ccacaccggc cttcgcgccc atctccatgc
     2701 tcatgttgca catggtcatc ctgccggaga tggacatggc atccaccacc gggcccacga
     2761 actccgcgca gcggtagtcg catccgtccg cccccatcat gccgatgaga tgcaggatga
     2821 cgtccttggc gacgaccggc ccgtccagcc tgccggtgag atggaagcgg aaggtccgcg
     2881 gcaccttcat ccaaagctcg cccgtggccc aaacggcggc catctccgtg gcccctatcc
     2941 cggtggagag ggcgcccagc gcaccatagg tggtggtgtg ggaatcggtc ccgacgatga
     3001 gcatgccggg gagcacgtgg cccttctccg gcagcacctg atggcatatc ccctcccgaa
     3061 ggtcgtagaa gtgcggcagg ccttcggtcc gcacgaaggc gcggacggcc tggtgcccct
     3121 ccgcggcctt gatggtattg gccgggaccc tgtgatcgaa gagcagcacc atcctttcgg
     3181 cgtcccacac ctccttggcc cccatctcat tgaaggcgcg gaggaccagg gcggcgttct
     3241 catgggacat ggcgaggtct atcttggcgg acactatctc accggggaat gcctcccttc
     3301 cgctggcacg tgatagtacc ttctgggcga aggtcatggc ctccatgggc ccttcatcgc
     3361 actcccccct atattcgttt ttcattgccc aggacccatt gctcgaaggc acaggcgttc
     3421 ctcaaggatg cgatcgggac gcgtagctcg atgtggacgc aacccaggca tatggaaagg
     3481 ctgatatcct cccatcctta aatcgggtct atgagcccga ggcgaagatg gttcctttgg
     3541 aggttcgtcg tctctgcgct gtccatcccc ttcgcccttt ccattttcat ccatgattgg
//
LOCUS       DAOSYN010000132         3556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_21047_length_3556_cov_4.226507, whole genome shotgun sequence.
ACCESSION   DAOSYN010000132 DAOSYN010000000
VERSION     DAOSYN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21047_length_3556_cov_4.226507"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLJ11_05720"
     CDS             <1..229
                     /locus_tag="PLJ11_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOO04204.1"
                     /translation="DAMGGIVKEVTVKELVNTLKEEKGPVSAVVFDGIITQRILDLAS
                     DLKIGTIVGTKMGNITKMPAGIDVWSKDDLN"
     gene            232..1740
                     /locus_tag="PLJ11_05725"
     CDS             232..1740
                     /locus_tag="PLJ11_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO04205.1"
                     /translation="MDGTEMGDWRSVECTSEVVIPGDPLQRVIGQEEAVTLARIAAKQ
                     RRHLLLVGPPGTGKSMIAQALALHLARPTQEIRVVHNPENPERPLVEVKGAEEVRIEM
                     RSRENAEGELMDPKNAPVNVAERLGYRCRNCGTYSSPKERICPKCQKGKGDVAANNPF
                     GDLMTGLFEQMNVAGLTGTAGKERVTTTRTRFGKEEVVVFERCGEMIRVLDQEALEKR
                     RDLEKRSPRKVIVRLDRNPFVLATGSSETELLGDVRHDPYGGHAQLGTQPYDRVVPGA
                     IHEAHEGVLFIDELPHLGHQQRFILTAMQEKRFPISGRNPQSAGASVKVENVPCDFIF
                     VGACNIQDLEQVLSPLRSRICGNGYEVLVNTAMPDTDANRGRLVQFIAQEIVSDGRIP
                     HADASAIEAIVREARGRAVKVDGINNALTLRLRELGGLIRAAGDLAVMEGAELIDAKH
                     IAKAVERSKSAEEQIKDRYGSYTQGLGTDISSAQKERSPYYFWNETKDSMYH"
     gene            complement(1737..2171)
                     /locus_tag="PLJ11_05730"
     CDS             complement(1737..2171)
                     /locus_tag="PLJ11_05730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOO04206.1"
                     /translation="MQLRRFQDRDLIDVWQVATESLREHYDPTLFTQLVPFWPEGLIV
                     IEDMGRTIAFIFGVLSGPQQARVLMLAVDRRFRNMGLGTLLTQELFRECAKKGVRQVS
                     LEVRASNIGAQRFYQRLGFFVVRRLAHYYSDGEDGILMMRYL"
     gene            complement(2197..>3556)
                     /locus_tag="PLJ11_05735"
     CDS             complement(2197..>3556)
                     /locus_tag="PLJ11_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOO04207.1"
                     /translation="YPEDGLPALVREVDLARESIELACYIMENWTLARHLAAAAARGV
                     AVTVLLEGQPVGGVSENGAAIAYHLQRAGTEVYVMRSSQSFRRYDYLHAKYVVFDHER
                     LLVSSENMADSSFTCNRGWAAMITSGPLALQASAVFHRDLAGKGLDVFPLETSIAPTE
                     GGPGRLLDIPSQRPVPVPASAALLTSPFELGEALTLLLERAQRRVLVQQLRIDEAWLD
                     DDGVLRSLLSAAERGVCVRILLDSGQGTKEENARVAARLNERAQEGNWDLQCRLVGGS
                     AGFDRLHNKGVIVDDTVLVGSANWVDTSMLENREMALSLRSEALARTFSDWFERDWKG
                     DASPPEIALPWHFIEVAEGEAVALDATGCSDASGVANITWDLDGDGVPDLSGPVQAVR
                     LPAGEHTICLTVEDALGNIAQETITVRVVPNGKGASAWLLYAPLPLLLLLPLLRRRNR
                     RV"
BASE COUNT          654 a         1173 c         1205 g          524 t
ORIGIN      
        1 cgatgccatg ggcgggatag tgaaggaagt gacggtcaag gagctggtca acaccttgaa
       61 ggaggagaag gggcccgtgt ccgcggtcgt cttcgacggc atcatcaccc agcggatcct
      121 ggacctggcc tccgacctga agatcgggac gatcgtcggc accaagatgg gcaacatcac
      181 caagatgccc gccggcatcg atgtctggtc caaggatgac ctgaactgaa tatggacggg
      241 accgagatgg gggactggcg cagcgtggag tgcacctccg aggtggtcat tccaggcgat
      301 cccctgcaaa gggtcatagg ccaggaggag gccgtgaccc tggcccgcat cgccgccaag
      361 cagcgccggc acctcctcct cgtcggcccc ccgggaacgg ggaagtcgat gatcgcacag
      421 gcgctggcgc tgcacctcgc ccgcccgacc caggagatcc gggtcgtgca taacccggag
      481 aacccggaga ggcccctggt cgaggtgaag ggcgcggaag aggttcgcat cgagatgcgg
      541 tcccgggaga acgccgaggg cgaactcatg gaccccaaga acgccccggt caacgtggcc
      601 gagcggctcg ggtacaggtg ccgcaactgc ggcacctact cctccccgaa ggagcgcatc
      661 tgccccaagt gccagaaggg gaagggcgac gtggccgcca acaatccctt cggggacctg
      721 atgaccgggc tgttcgagca gatgaacgtg gccggcctca ccggaacggc cgggaaggag
      781 cgcgtgacga cgacccggac ccgcttcggc aaggaggagg ttgtcgtctt cgagcggtgc
      841 ggcgagatga tcagggtgct ggaccaggaa gcgctggaga agcggaggga tttggaaaag
      901 cggtcgccgc ggaaggtcat cgtgcgcctg gaccgcaacc cattcgtgct ggcaaccggc
      961 tcctccgaga cggagctgct gggcgatgtg cgccacgacc cgtacggagg gcatgctcag
     1021 ctggggacgc agccctatga ccgcgtcgtc ccgggcgcga tccacgaggc gcacgagggg
     1081 gtcctcttca tagacgagct gccgcacctg gggcatcagc agcgcttcat cctgacggcc
     1141 atgcaggaga agcgcttccc catctccggc cgcaacccgc agagcgccgg ggcgagcgtg
     1201 aaggtggaga acgtcccctg cgacttcatc ttcgtggggg cgtgcaacat ccaggacctg
     1261 gagcaggtcc tctcccccct gcgctcccgg atatgcggga acgggtatga ggtgctcgtc
     1321 aacaccgcca tgcccgacac ggacgcgaac cgcgggcggc tggtgcagtt catcgcccag
     1381 gagatcgtgt ccgatgggcg catcccgcac gcggacgcgt cggcgatcga ggcgatagtc
     1441 agggaggcga gggggagggc cgtcaaggtc gacggcatca acaacgccct gaccctccgg
     1501 ctcagggagc taggcgggct gatccgggcg gcgggggacc tggcggtgat ggagggggcc
     1561 gaactcatcg acgccaagca catcgccaag gcggtggagc gcagcaagag cgcggaggag
     1621 cagataaagg accgctacgg ctcgtacacc caaggcctgg gcaccgacat ctcgtcggcg
     1681 cagaaggagc gctcccccta ctatttctgg aacgagacca aggactcgat gtaccactag
     1741 aggtagcgca tcatgaggat gccgtcctcc ccgtcggagt aatagtgcgc caggcggcgg
     1801 acgacgaaga acccgagcct ctggtagaac cgctgcgccc cgatgttgct ggccctcacc
     1861 tcaagcgaga cctggcgcac gcccttcttg gcgcactcgc ggaagagctc ctgggtcagc
     1921 aacgtcccca gccccatgtt ccggaaccgg cggtcaaccg cgagcatcag gaccctcgcc
     1981 tgctgcggtc cgctgaggac cccgaagatg aaggcgatgg tcctccccat gtcctcgatc
     2041 acgatcagcc cctccggcca gaacggcact agttgcgtga agagcgtggg gtcgtagtgc
     2101 tcgcggaggg attccgtggc tacctgccat acatcgatca ggtcgcggtc ctggaaacgg
     2161 cggagctgca ccagcggccg aacgccttcc gcctaactaa acccttctgt tcctacgccg
     2221 cagaaggggc agcaggagaa ggaggggaag gggggcgtac agcagccatg ccgatgcccc
     2281 cttcccgttc ggaacgaccc ttacggtgat cgtctcctga gcgatgttcc cgagggcgtc
     2341 ctcgaccgtc aggcagatcg tatgctcccc tgcgggaagg cgcacggcct gcaccggccc
     2401 ggagaggtcc gggaccccgt ccccgtccag gtcccacgtg atgttcgcga cccccgaggc
     2461 gtcggagcag cccgtggcat cgagggccac cgcctccccc tcggccacct cgatgaagtg
     2521 ccagggaagg gcgatttcgg gaggggaggc gtcccccttc caatcgcgct cgaaccaatc
     2581 cgagaacgtc ctggcaagcg cttcggagcg gagcgagagc gccatctccc tgttctccag
     2641 catggaggtg tccacccagt tcgcgctccc cacaaggacg gtatcgtcca cgatcacccc
     2701 tttgttgtgc aaccggtcga agccggccga tccgccaacc aggcggcatt ggaggtccca
     2761 gttgccctcc tgggcccgct cgttcagcct ggcagcgacc cgggcgttct cctccttcgt
     2821 cccctggccc gagtccaaca ggatgcggac gcagaccccc cgctccgctg cggagaggag
     2881 ggaacggagg acgccgtcgt catcgagcca cgcctcatcg atgcgcagct gctgtaccag
     2941 cacgcgccgc tgcgccctct cgagcagcag cgtcagcgcc tccccgagct cgaagggcga
     3001 ggtgagcagc gccgcagagg cgggcaccgg gaccggccgt tgggacggga tgtcgagcag
     3061 ccttcccggt ccgccctcgg tcggagcgat gctcgtttcc agggggaaca cgtccaggcc
     3121 tttgcctgcc aggtcccggt ggaacaccgc gctcgcctgg agggcgagcg ggccgctcgt
     3181 gatcatcgcg gcccagccgc ggttgcaggt gaagctggag tcggccatgt tctccgagga
     3241 gacgaggagg cgctcgtgat cgaacacaac gtacttggcg tgcagatagt cgtaccggcg
     3301 gaacgattgg ctggagcgca tcacatatac ctccgtgcct gcgcgctgga gatggtacgc
     3361 gatcgccgcg ccgttctcgg acaccccgcc caccggctgc ccttcgagga ggaccgtgac
     3421 ggcgacgccc cgcgcggcgg cggccgccag atgccttgcc aaggtccagt tctccatgat
     3481 gtagcaggcg agctcgatgc tttcgcgggc taggtcaacc tccctcacca gggcgggcag
     3541 gccgtcctcc gggtac
//
LOCUS       DAOSYN010000133         3555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_21054_length_3555_cov_4.394571, whole genome shotgun sequence.
ACCESSION   DAOSYN010000133 DAOSYN010000000
VERSION     DAOSYN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21054_length_3555_cov_4.394571"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..513)
                     /locus_tag="PLJ11_05740"
     CDS             complement(103..513)
                     /locus_tag="PLJ11_05740"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOO04208.1"
                     /translation="MAETKRFAMVVSEGTFDKAMMAMMMGNTAASMGIETHIFFTFFG
                     LNLLKKGTKPKLPGMMRFFTGMMVKKMKGIGMDGYQEQLAMAKELGVKLYACSTSMEM
                     MGIKKEDLIDGITVLGAAAFLNIATDADMQLFIG"
     gene            complement(536..766)
                     /locus_tag="PLJ11_05745"
     CDS             complement(536..766)
                     /locus_tag="PLJ11_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOO04209.1"
                     /translation="MDKNDTLDAKGLMCPMPIVKLAKKMKEMPVGAVLELIADDVGSK
                     EDVPAWCKRTGNELVGQKDEGGVYYYYIKKVQ"
     gene            856..1221
                     /locus_tag="PLJ11_05750"
     CDS             856..1221
                     /locus_tag="PLJ11_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO04210.1"
                     /translation="MPESIKDVNSCGDLIQCAFSLNGFEVEVYETLLTTGPLSANDLA
                     DRMGKDRSTVYRALQKMLSCGMVYRETRSIPRGGYYHVYRAIPRSELRERLEGCVEGW
                     YRKMRAVLEQFGEEGGKPL"
     gene            1349..2263
                     /gene="pyrB"
                     /locus_tag="PLJ11_05755"
     CDS             1349..2263
                     /gene="pyrB"
                     /locus_tag="PLJ11_05755"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOO04211.1"
                     /translation="MDIVSIRDLSREQIEEVMERSERMIPYARGEKASKALDGKILAT
                     LFFEPSTRTRMSFESAMMRLGGRITDLGVPSMSSISKGESLADTIRMVEVYSDIIVIR
                     HPREGAARLAARFSSKPVINAGDGAGQHPTQTLLDLFTIKRIKKDFNKLNVVMVGDLK
                     YGRTVHSLAEALSSFGVELTFVAPAALQMPKETVRQVERMGGRPKLTNRLEETIADAD
                     VLYVTRIQKERFPDLAEYQKVAGMYRVDRRLLREAKSDLIVLHPLPRVDEIDPEVDGT
                     PHAKYFEQAFNGVPVRMALLNLVLGGEL"
     gene            2263..2724
                     /gene="pyrI"
                     /locus_tag="PLJ11_05760"
     CDS             2263..2724
                     /gene="pyrI"
                     /locus_tag="PLJ11_05760"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOO04212.1"
                     /translation="MKEFKVTPIRNGTVIDHIECGMALKVLRIIGMADGGKAQSPVSV
                     LMHVPSKKEGWKDVVKVEDRELDPREVDKIALIAPKATINIIRDFNVAEKYNVKMPER
                     VVGKARCSNPNCITNQNEPVEPEFVVESKNPPVLRCVYCDRQLDDIAGNLL"
     gene            2750..3280
                     /locus_tag="PLJ11_05765"
     CDS             2750..3280
                     /locus_tag="PLJ11_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO04213.1"
                     /translation="MGVLILTGMPGAGKEELVTIALALGYQVQRMGDVVRAEAVRNGV
                     PPQAVGEFADRERRSHGYDIWAKRTVPLVGEGDTLIDGCRSMAEVNVFRQAFGGHVKV
                     VAVHSAPSTRFPRLVARGRPDAPRDRREFDERDRRELGWGLGDTIALADLMLVNEGTL
                     EDFHRDARSLLMELRG"
     gene            3277..3546
                     /locus_tag="PLJ11_05770"
     CDS             3277..3546
                     /locus_tag="PLJ11_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04214.1"
                     /translation="MRFKVERICRSDSMSVVPLEAVRFDLQASAERLRREGYAAEPNE
                     LYLVTRMGEVDVTIYPSGRVLVRPMEDKAGAKELAQRLFALMVRE"
BASE COUNT          674 a         1073 c         1178 g          630 t
ORIGIN      
        1 gcccgctcct ggaccccttg gcgagggccc gcaccagctc cagatcgccg aggtcgtgcg
       61 gggacttcag cgttatgaac agcttggacc tcatggccat cctcagccta tgaaaagctg
      121 catgtccgca tcggtagcga tgttcaggaa ggcggccgcc cccaggacgg ttatgccgtc
      181 gatgaggtcc tccttcttga tgcccatcat ctccatggag gtgctgcagg cgtagagctt
      241 gacgcccagc tccttggcca tcgccagctg ctcctggtag ccgtccatcc cgatgccctt
      301 catcttcttg accatcattc cggtgaagaa gcgcatcatg cccggcagct tgggcttcgt
      361 ccccttcttc aggaggttga gcccgaagaa cgtgaagaag atgtgcgtct cgatccccat
      421 gctcgcggcc gtgttgccca tcatcatggc catcatggcc ttgtcgaagg tgccctcgga
      481 caccaccatt gcgaacctct tggtctctgc cattgtcatc ccctcatcct ttttctcact
      541 ggaccttctt tatgtaataa taatagacac cgccctcgtc cttctggccc accagctcgt
      601 tgccggtccg cttgcaccat gcgggaacgt cctccttcga gccgacatcg tccgcgatga
      661 gctccagcac cgcgcccacg ggcatctcct tcatcttctt ggccaatttc acgatcggca
      721 tcgggcacat caggcccttg gcatcaagcg tatcgttctt atccatagga ctcaccatag
      781 cggttttgca cggttgtgca acaattatat ataactgttg gtttataagc ttttcttaag
      841 gaaggagcgg agccgatgcc ggagtccata aaggacgtga acagctgtgg ggacctcatc
      901 cagtgcgcct tcagcctgaa cgggttcgag gtcgaggtgt acgaaacgct attgaccacc
      961 ggaccgttga gcgccaacga tctggcggac cggatgggca aggaccgctc caccgtctac
     1021 cgagcgctcc agaagatgct gagctgcggg atggtgtacc gagagaccag gagcatcccc
     1081 cgcgggggct attaccatgt gtatagggcc atcccaagga gcgagctccg cgagaggctg
     1141 gaggggtgtg tggagggatg gtaccgcaag atgagagcgg tcctggaaca gttcggggag
     1201 gagggcggca agcccctgta gcgcatcccc gccccaatcg ccccctctcg gtccgatgtt
     1261 gaccgggcct aggcgttgcc ccccggccat gccaagggta aaatacggtc gtgaggattc
     1321 aacaccgaga gtcatgagct ttgaaggaat ggacatcgtg tcgatccgcg atctgagccg
     1381 ggagcagatc gaggaggtta tggaacggtc cgagagaatg atcccctacg cccgggggga
     1441 gaaggcctcc aaggcattgg acggaaagat cctggccacc ctgttcttcg agccgtccac
     1501 ccgcaccagg atgtccttcg agagcgccat gatgcgcctc ggcggcagga taacggacct
     1561 gggcgtccct tccatgagct ccatctccaa gggggagtcc ctggcggaca ccatccggat
     1621 ggtggaggtc tattcggaca tcatcgtcat caggcacccg cgggagggcg cggccaggct
     1681 ggccgccagg ttctcgtcca agcccgtcat caacgcgggc gacggggccg ggcagcatcc
     1741 cacccagacg ctgctcgacc tgttcaccat caaacgcatc aagaaggatt tcaacaagct
     1801 caacgtggtc atggtcgggg acctcaagta cgggcgcacg gtccattccc tcgccgaggc
     1861 gctgagctcc ttcggcgtgg agctgacctt cgtggcgccc gccgcgctgc agatgcccaa
     1921 ggagacggtc aggcaggtgg agcggatggg cggaaggccc aagctgacga accgcctgga
     1981 ggagaccatc gccgacgcgg acgtgctgta cgtcacccgc atccagaagg agaggttccc
     2041 cgacctggcg gagtaccaga aggtggccgg gatgtaccgc gtggaccgcc ggctcctgcg
     2101 cgaggccaag agcgacctca tcgtcctgca tcccctcccc cgggtggacg agatcgaccc
     2161 ggaggtcgat ggcacgccgc atgccaagta cttcgagcag gcgttcaacg gggtgccggt
     2221 gcgaatggcg ctcctcaacc tggtcctggg aggcgagctt tgatgaagga gttcaaggtg
     2281 accccgatcc gcaacgggac ggtcatagac catatcgagt gcgggatggc gctgaaggtg
     2341 ctgcgcatca tcggcatggc cgacgggggg aaggcgcagt ccccggtcag cgtgctgatg
     2401 cacgtcccct cgaagaagga ggggtggaag gacgtggtga aggtcgagga ccgggagctg
     2461 gaccccaggg aggtggacaa gatcgcgctc atcgcgccga aggccaccat caacatcatc
     2521 cgggacttca atgtggcgga gaagtacaac gtaaagatgc ccgagcgcgt ggtaggcaag
     2581 gcccggtgca gcaatcccaa ctgcataacc aatcagaacg agccggtgga gccggagttc
     2641 gtggtcgagt cgaagaaccc gccggtgctg cgctgcgtct actgcgatcg ccagttggac
     2701 gacatcgcag ggaacctgct gtgagcgccg ttcccttttg aaggctgaga tgggcgtcct
     2761 gatcctgacc ggaatgcccg gggcgggcaa ggaggagctc gtgacgatag ctctggcgct
     2821 aggataccag gtccagcgca tgggggacgt ggtgcgggcc gaggccgtca ggaacggcgt
     2881 gcccccgcag gccgtgggcg agttcgcgga cagggagagg cggtcccatg ggtacgacat
     2941 ctgggccaag cgcaccgtcc ccctggtggg cgagggcgac acgctcatcg acggctgccg
     3001 gagcatggcc gaggtgaatg tgttccgcca ggcgttcgga gggcacgtca aggtcgtggc
     3061 ggtgcacagc gccccctcca cccgattccc gcggctggtg gcccggggaa ggcccgacgc
     3121 gccccgcgac cggagggagt tcgatgagcg ggaccggcgg gagctgggat gggggctggg
     3181 ggacaccata gccctggcgg acctgatgct ggtgaacgag ggcacccttg aggatttcca
     3241 ccgggatgcc cgctccctgc tcatggagct gaggggatga ggttcaaggt ggagcgcatc
     3301 tgccgctccg attccatgtc cgttgtcccc ctggaggcgg tgcggttcga cctgcaggcc
     3361 tcggcggagc ggttgcggag ggaggggtac gccgcggagc cgaacgagct ctacctggtg
     3421 accaggatgg gggaggtgga cgtgaccatc tacccgagcg gcagggtgct cgtgcgcccc
     3481 atggaggaca aggccggcgc caaggagctt gcgcagaggc tgttcgcgct catggtccgg
     3541 gaatgagcgc ccttc
//
LOCUS       DAOSYN010000134         3548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_21106_length_3548_cov_3.016032, whole genome shotgun sequence.
ACCESSION   DAOSYN010000134 DAOSYN010000000
VERSION     DAOSYN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21106_length_3548_cov_3.016032"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLJ11_05775"
     CDS             <1..678
                     /locus_tag="PLJ11_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04215.1"
                     /translation="CKAGKAQLLKVHLKDVSGFEDLAEYDIVVPPERARQVLGTEWEA
                     FLKRNRLDQEMEMVYLDKVKNEADRQLLAAEGRKRYTGWIALDKVPADRLPALMERAG
                     KDDRLTGWDMLAFDEMAATCARCPLSWDEGRGCMGTFGPENSALPGIAQKHNCATVAG
                     VPSAIEGRKVFSVEDAARLLEEVKVLRERLPEEGKVMVRRYTGVLDRLERMGNVCVTY
                     GTRFYFL"
     gene            complement(994..1986)
                     /locus_tag="PLJ11_05780"
     CDS             complement(994..1986)
                     /locus_tag="PLJ11_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HOO04216.1"
                     /translation="MRLQPRQDWDLWAIISLSLLLIVVIYAFPGSAARIVLGLPFILF
                     FPGYAALSVLFPEGKDLEIIERIALSFALSIAISPLVGFGLNYTPFGIRLAPILFSLA
                     SLNVALSVLGLYRRERAQSPYLPFDPVATWKRYFGQFKAEKGLDKALTVVLVISILSS
                     VVALVYVVAVPRQGESFTEFYILGPSGNATDYPRNLTVGEDASVIIGIANHEHRTVTY
                     TVELWLVNMSFENNVTNVHAMYFFDSFNVTLEHTDVDLEGEWQPQWERPYHFNVSVEG
                     NFKLWFLLYKDEAPSLPFPPAYMMDYTGTSAEERIVAAVENKVQSLNLNLRIGE"
     gene            2083..2907
                     /locus_tag="PLJ11_05785"
     CDS             2083..2907
                     /locus_tag="PLJ11_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04217.1"
                     /translation="MALCELFELDDLELIEGTFEELSNERFLAHMLRGSREVRLLVSH
                     DERPLAMAREDNGWKAVSFLWKEPSDELIEALPELDLKVYTCDTRTYREAVCDHFSRR
                     LLDEVEPAMEDVPEDRAKKVSDLIWKEWDFRPGELCYDCCCGSGVGSLALSKVGLQSF
                     AFDVDQALLSSGLRKGRLKTWSTVQLDAMKASQYLERASYAILLMAGNIGPGNAWMWE
                     RIFDQVLRIADHVLATVGTQEEAEQLRAWAEARGRRPRVFENERDLFYDRWACSID"
     gene            2997..>3548
                     /locus_tag="PLJ11_05790"
     CDS             2997..>3548
                     /locus_tag="PLJ11_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOO04218.1"
                     /translation="MTDYANCSRIIMDVLELKTEPVAVTLVRKGQQLPKGYEIPEKNI
                     RHCQSVMRARKGEKLLLTPDKHACHVGASALGMVPIPDKVRSGEFHFNLGMFANQEAA
                     RRMIEERPQLEVGDIIATAVSPLSKAEIPPDVVIVTGTPEQLYWLVPVAAKYFEGGKM
                     LIEMETFQASCAYSTVYPYLTGTL"
BASE COUNT          780 a          974 c         1143 g          651 t
ORIGIN      
        1 tgcaaggcgg gcaaggccca gctgctcaag gtccacctga aggacgtctc gggcttcgag
       61 gacctcgcgg agtacgacat cgtggtgccg cccgagcggg ccaggcaggt ccttggcacg
      121 gaatgggaag cgttcttgaa aaggaacagg ctggaccagg agatggagat ggtgtacctg
      181 gacaaggtca agaacgaggc ggaccggcag ctgctggccg ccgaaggccg aaagcgctac
      241 accggctgga tagcattgga caaagtgccc gcggatcgcc tgcccgcgct catggaaagg
      301 gccgggaagg acgatcggct caccggctgg gacatgctgg cattcgacga aatggcagcc
      361 acctgcgcca ggtgcccgct gtcctgggac gagggcaggg gatgcatggg gaccttcggc
      421 cccgagaaca gcgccttgcc agggatagcc cagaagcaca attgcgccac ggtcgccggc
      481 gtcccttccg ccatcgaggg gcggaaggtg ttctcggtcg aggatgccgc caggctcctg
      541 gaggaggtca aggtcctgcg ggagagactg cctgaggagg gcaaggtgat ggtccgccgg
      601 tacacagggg ttctggaccg cctggagagg atggggaacg tctgcgtgac ctacgggacc
      661 cgcttctatt tcctttaggc ctgcagtgcg caaacctttt ttctctcctt ttcatccttc
      721 atccttgatg agcggtaacg gtgccgggcc atgctggccc tgacggtccg attcctttca
      781 atgaaagcga agcgctccta gtgaacggac ggcttatgga gctcaaaggg ctcagggagc
      841 tgatggagga ggtccgtagg ctcagctgcc aaacgagggg agtggccggc atactgatgg
      901 ctcaagcgaa taaacccaac gcagtccagg caggggcatt atcaggacgc ccctaaggac
      961 ggatgcgcca ggctgcacct tggcgtaaca gtatcattcg ccgatccgca ggttcaggtt
     1021 caggctctgc accttgttct ccacggccgc cacgatccgc tcctcggcgg aggtgcctgt
     1081 atagtccatc atgtaggcgg gaggaaatgg aagtgagggc gcctcgtcct tatagagcag
     1141 gaaccagagc ttgaaattac cttcgacgga gacgttgaaa tggtacggac gctcccattg
     1201 aggctgccat tccccctcga ggtccacatc ggtatgctcc agcgtgacgt tgaaagaatc
     1261 aaagaagtac atggcgtgca cgttggtcac attgttttcg aagctcatgt tgacgagcca
     1321 tagctccacg gtgtacgtca cggtgcggtg ctcgtggttg gctatgccta tgatgaccga
     1381 cgcgtcctcc ccgaccgtta ggttcctggg atagtcagtg gcattcccgc tcggtcctag
     1441 gatgtagaac tccgtgaagc tctctccctg ccgggggacg gccacgacgt agaccagggc
     1501 gaccaccgag gacaggatcg agatgacaag gacgacggtc agcgccttgt ccagtccctt
     1561 ctcggccttg aactggccaa agtatctttt ccaggtcgcg acggggtcga atgggaggta
     1621 tggggattga gcgcgttccc gacggtagag gcccaggaca ctcaacgcca cgttgagcga
     1681 tgccaaggag aacagtatcg gggcgagcct gatcccgaag ggggtgtagt tcagaccgaa
     1741 gccgaccaag ggggatatgg cgatgctgag ggcgaaggaa agggcgatcc gctctatgat
     1801 ctcaaggtcc ttgccctccg ggaacagcac cgacagtgcg gcatagccgg gaaagaacag
     1861 gatgaagggg agccctaata caatccgagc ggcgctcccc ggaaacgcgt atatcaccac
     1921 tatgaggagc agggacaggg agatgatcgc ccacaggtcc cagtcctgcc taggctgaag
     1981 cctcatcctt cagggggagt gaatgacgct atttatcgct ttgcggctca tcgccctcct
     2041 cgaccagttt tttaatattt cgaactccgt taccactcta ccatggcgct gtgcgagctt
     2101 ttcgaactgg acgacctgga actgatagag gggaccttcg aggaactgag caacgagagg
     2161 ttcctggcgc acatgctgcg cggctctcgg gaggtccggc tcctcgtcag ccatgacgaa
     2221 aggcccttgg ccatggccag ggaggacaac ggctggaagg cggtaagctt cctatggaag
     2281 gagccttcgg acgagctgat cgaggccctt cccgagctgg acctgaaggt gtatacgtgt
     2341 gacacgagaa cgtaccgcga ggcggtgtgc gaccacttct ccagaagatt actggacgag
     2401 gtggagccgg ccatggagga cgtgccagag gatagggcga agaaggtctc ggacctcatc
     2461 tggaaggaat gggacttcag gcccggcgaa ctctgctacg attgctgctg cggttccggg
     2521 gtgggaagcc tggccttatc caaggtggga ctgcagtcct ttgccttcga cgttgaccag
     2581 gcattgctct cgtcaggcct ccgaaagggc cggttgaaga cctggagcac tgtccagctc
     2641 gatgccatga aggcctccca atacttggag agggcgtcct acgcaatatt gctgatggca
     2701 gggaacatcg gtccaggaaa tgcgtggatg tgggaaagga tcttcgacca ggtgcttcgg
     2761 atagccgacc acgtcctggc gaccgtcggc acccaggaag aggcggagca gctaagggcc
     2821 tgggcggagg ccaggggaag gaggccccgg gtcttcgaga acgagcggga cctcttctac
     2881 gaccgctggg cgtgctcgat cgattaacgg ccctgatggt ccttcttcaa ttaccaatcg
     2941 aataataaaa gggaatacaa taaatgttaa aaaccagcaa ccccctcgat atcatcatga
     3001 ccgattacgc caactgttcc cgcatcatca tggacgtgct agaactgaag acagagccag
     3061 tagccgtcac actggtgagg aaggggcaac agttgcccaa ggggtacgag atcccggaaa
     3121 agaacatacg ccattgccag tccgtcatga gggccaggaa gggggagaag ctgctgctga
     3181 caccggataa gcacgcttgc catgtgggcg cctcggcgct cgggatggtg cccatcccgg
     3241 acaaggtgag gagcggggag ttccacttca acctgggcat gttcgccaac caggaagcgg
     3301 cgaggagaat gatagaggag cgcccccaac tggaggtggg ggacatcatc gctaccgcgg
     3361 tgtccccgct cagcaaggcg gagatacccc cggatgtggt gatcgtcacc gggaccccag
     3421 agcagctcta ttggcttgta ccggtcgcag ccaaatactt cgagggcggc aagatgctca
     3481 ttgagatgga aacgttccag gcgagctgcg cctattccac cgtatatcca tacctcaccg
     3541 gcacgctc
//
LOCUS       DAOSYN010000135         3538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_21160_length_3538_cov_3.942865, whole genome shotgun sequence.
ACCESSION   DAOSYN010000135 DAOSYN010000000
VERSION     DAOSYN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21160_length_3538_cov_3.942865"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PLJ11_05795"
     CDS             <1..920
                     /locus_tag="PLJ11_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOO04219.1"
                     /translation="GLEMEEVTDQVRAAPYEIFQKVLSKGGMVTCINLKASMLREQDA
                     DGAVGRKQVDRLIEWAKGQGMGGLTWMRMTPEGLSSNIVKYFPVEVRKAIAARTAAEP
                     GDLLLFLAGPREGTLRAGGQLRLKLARDLGLLEGKGHQFVWVVSCPLFQRDPATGGLT
                     SFHHPFVRPVGDVIPEGKECATAMGRSYDLVLDGSEIGSGSLRNHDPAMQRRVFSILG
                     MDEATMQREFDFFLEALEYGAPPHGGIGMGIDRLCAMLLGCESIRDVIAFPKNKKFQS
                     LVDGAPTKVEEAKLSELQLLSLAEDDDGA"
     gene            complement(927..2261)
                     /gene="gatB"
                     /locus_tag="PLJ11_05800"
     CDS             complement(927..2261)
                     /gene="gatB"
                     /locus_tag="PLJ11_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOO04220.1"
                     /translation="MKIGLEMHFQLPTRSKLFCPCPTAPAEPNHNICPVCLGYPGSRP
                     SLNRKALEIGLGIAQFLDCRIEERVWFSRKTYFYPDLPKNFQITQYETPLGTDGRFEL
                     NGRSIGIWRVHLEEDPGRIRRVGRPGEEIAFVDYNRSGIPLVEIVTAPDLTSPEEART
                     FIDQLVIELRALCGLEAQDEQTVRVDANISVGEERVEVKNVQGLRNLERALRFEAGRQ
                     SKLIAAGKRVVRETRRFDEERKVTTSAREKETEEDYGYIGEPDLGEFRTGNMLRALDL
                     KETPLARARRLVASYGIAEVTAKQLVLTSSRLTDLFEELCHHVPAERAVAWTTGPISG
                     NWSALQDQAAWPELVQIVKADAEGHINDIEAKRRILALAGVRQEEASGTEDDLSKLIS
                     DYIDAHPEVLADYRRNPKAANTVIGHAMKESRGRHSSKEVVEEVKRQMERRA"
     gene            complement(2258..>3538)
                     /locus_tag="PLJ11_05805"
     CDS             complement(2258..>3538)
                     /locus_tag="PLJ11_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOO04221.1"
                     /translation="QRRHLAFTETVDGLDEGRFHFSAKDNLCALGFQARAGSAVLEGY
                     RPPFDATPIHRLKAAGGQLVGKTTMDEFGFGSFSTNSAHGVPLNPFDPDRSCGGSSGG
                     AGAAACLIPGHIALGVSTGGSISGPASFCGVVGLTPTYGRVSRYGLIDYGNSLDKVGL
                     LSRSAASIAQHFGTIAGPDPKDPTSCAQPPLAGKGRMPSSLALPREAMENLDPAVRKA
                     FQGALPVLRGMGVAVEEVSMPSLRFALPAYYVLATSEASTNLARYCGMRFGRREEELN
                     QHFNDFFTGVRSEGFGDEAKRRILLGTFCRMVGFRSRYYLKSLAVRQMLIGEYRSVFQ
                     EHDAVLTPTMPFLPPRFEDIRRMTPLEMYLADFLTIPPNLVGMPHVSVPCGYVDGLPV
                     GMQVVADHWKEMDLLELAKGWEGAFPYTFPEVRP"
BASE COUNT          575 a         1129 c         1219 g          615 t
ORIGIN      
        1 tcgggttgga gatggaggag gtgaccgacc aggtgagggc cgctccctac gagatattcc
       61 agaaggtcct ttccaaggga ggcatggtca cctgcataaa cctgaaagcc tcgatgctga
      121 gggagcagga cgccgacggc gccgtcggca ggaagcaggt cgaccgcctg atcgaatggg
      181 ccaagggcca gggcatgggc gggctcacct ggatgcggat gaccccggag gggttgagtt
      241 ccaacatcgt gaagtacttt cctgtggagg tgcggaaggc catcgcggcc cgcaccgccg
      301 ccgagcccgg ggacctgctg ctgttcctgg ccggccccag ggagggcacg ctcagggccg
      361 ggggccagct ccgcttgaag ctggccaggg atctggggct cctcgagggg aagggacacc
      421 agttcgtctg ggtcgtttcc tgccccctgt tccagaggga ccctgcgacg ggaggcctca
      481 cctccttcca ccaccccttc gtgagaccgg tcggggatgt catcccggag ggcaaggagt
      541 gcgccaccgc catggggcgc tcgtacgacc tggtgctgga tggcagcgag ataggctcgg
      601 gctcgctgag gaaccacgac ccggccatgc agcgcagggt gttcagcatc cttgggatgg
      661 atgaggccac aatgcagagg gagttcgact tcttcttgga ggcgttggag tacggcgcgc
      721 ccccgcacgg ggggataggc atgggcatcg accgcctgtg cgccatgctc ctcggctgcg
      781 agagcatccg ggacgtgatc gccttcccga agaacaagaa gttccagtcc ctcgtggacg
      841 gcgcgcccac gaaggtggag gaggccaagc tctccgagct gcagctcctt tccctggccg
      901 aggatgacga tggggcctag ggtccgctag gccctccgtt ccatctgcct tttgacctct
      961 tcgaccacct ctttggagga gtgccgcccc ctgctctcct tcatcgcgtg cccgatgacg
     1021 gtgttggcgg ccttggggtt cctccgatag tcggccagga cctccgggtg ggcgtcgatg
     1081 tagtcggaga tcagcttgga gaggtcatcc tccgtgccgg aggcctcctc ctgccgcacc
     1141 cctgccaggg ccaggatgcg gcgcttggcc tcgatgtcgt tgatgtgtcc ctccgcgtcg
     1201 gccttcacta tctggaccag ctccggccat gcggcctggt cctgcagggc cgaccagttc
     1261 cccgagatag gtccggtggt ccatgcgacc gccctttccg cgggaacgtg atggcagagc
     1321 tcctcgaaca ggtccgttag ccgggacgac gtcaggacga gctgcttggc cgtgacctcc
     1381 gctatcccat aggaggcgac gaggcgcctg gcccttgcca gcggcgtctc cttaaggtcc
     1441 agggcccgga gcatgttccc ggtccggaac tcgcccaggt ccggttcccc tatgtacccg
     1501 tagtcctcct cggtctcctt ctcccgggcg gaggtggtca ccttgcgctc ctcgtcgaag
     1561 cggcgggtct ccctcaccac cctcttgccg gccgcgatga gcttggactg ccggcccgcc
     1621 tcgaatctca gggccctttc caggttccgc aggccctgca cgttcttgac ctccaccctc
     1681 tcctccccga cggagatgtt ggcgtccacc cggaccgtct gctcgtcctg ggcctccagc
     1741 ccgcagaggg cgcgcagctc gatcaccagc tggtcgatga acgtccttgc ctcctcgggg
     1801 gaggtgaggt cgggagcggt gacgatctcc accagcggta tgccggagcg gttgtagtcc
     1861 acgaaggcga tctcctcccc ggggcggccc accctcctta tccggccagg gtcctcctcg
     1921 aggtgcacgc gccagatgcc gatgctccgg ccgttcagct cgaagcgccc gtccgtgccc
     1981 aacggtgttt cgtactgcgt gatctggaag ttcttcggca ggtcgggata gaagtaggtc
     2041 ttgcgggaga accacaccct ctcctctatc ctgcaatcca ggaactgggc tatgcccagc
     2101 cctatctcca gggccttgcg gttcagcgaa ggcctggagc ccgggtaccc gagacagacc
     2161 gggcagatgt tgtggttcgg ctcggcgggg gcggtcgggc aggggcagaa gagcttggag
     2221 cgggtgggaa gctggaagtg catttccaag cctattttca tggccgcacc tccgggaagg
     2281 tgtacgggaa cgcgccttcc caccccttgg ccaattccag aaggtccatt tccttccagt
     2341 ggtcggcgac cacctgcatc ccgaccggaa ggccgtcgac gtagccgcag gggacggaca
     2401 cgtgcggcat cccgaccagg ttcgggggga tggtgaggaa gtcggccagg tacatctcca
     2461 acggcgtcat gcgcctgatg tcctcgaagc ggggcgggag gaagggcatg gtcggcgtga
     2521 ggacggcgtc atgctcctgg aacacactgc ggtactcgcc gatcagcatc tggcgcacgg
     2581 ccaacgattt caggtagtac cgggagcgga agccgaccat gcggcagaag gtgccgagca
     2641 ggatgcgtct cttggcctcg tcgccaaagc cctcggagcg gacgccggtg aagaaatcgt
     2701 tgaaatgctg gttgagctcc tcctccctgc gcccgaagcg catgccgcag tagcgggcaa
     2761 ggttcgtgga ggcttccgag gtcgccagga cgtagtaggc cggaagggcg aagcgcaggg
     2821 agggcatgga gacctcctct acggccacgc ccatccccct caggacgggg agcgccccct
     2881 ggaaggcctt gcgcaccgcg gggtcaaggt tctccatggc ctcccttggg agggcgaggg
     2941 agcttggcat cctccccttc ccggcaaggg gcggttgagc gcaggacgta gggtccttgg
     3001 ggtcggggcc tgcgatcgtt ccaaagtgct gtgcgatcga tgcggccgat ctggagagca
     3061 ggcccacctt gtccaacgag ttgccgtagt caatcagacc atagcgggag acccggccat
     3121 acgtgggggt caggcccacc accccgcaga aggaagcggg gccggagatg gagcctccgg
     3181 tggagacacc gagggcgatg tgcccgggga tcaggcaggc ggccgcgccg gcgcccccgg
     3241 aggacccccc gcagctgcga tccggatcga aggggttgag aggcacgccg tgggcggagt
     3301 tcgtagagaa ggaaccgaag ccgaactcgt ccatcgtggt cttgccgacc agctgcccgc
     3361 ccgctgcctt gagtcggtgg atgggggtgg cgtcgaacgg ggggcggtag ccctcgagca
     3421 cggccgaacc tgcccgggcc tggaagccca gggcgcacag gttgtccttg gcggagaagt
     3481 ggaaacggcc ctcgtccagc ccatcgaccg tctcggtgaa ggcgaggtgc ctgcgctg
//
LOCUS       DAOSYN010000136         3484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_21511_length_3484_cov_4.178478, whole genome shotgun sequence.
ACCESSION   DAOSYN010000136 DAOSYN010000000
VERSION     DAOSYN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21511_length_3484_cov_4.178478"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLJ11_05810"
     CDS             <1..444
                     /locus_tag="PLJ11_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOO04222.1"
                     /translation="TKGEPGTGNIIEAVRHQRIIMGKVRELKGKDEAELAVIARQIEA
                     PFYLIKEVAQLQRLPVINFAAGGVATPADAALMMQLGSDGVFVGSGIFKSADPAVRAK
                     AIVEAVTHYDDPKAIAEVSKGLGEAMHGIEISTLPKEQRMQERGW"
     gene            complement(505..1575)
                     /locus_tag="PLJ11_05815"
     CDS             complement(505..1575)
                     /locus_tag="PLJ11_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HOO04223.1"
                     /translation="MVPKSKILKVLDEYDKSKLTVATLCSHSSLQIFNGAKKEGFRTL
                     GLTVKDNFRYYDAFPLGRPDEFLKLDSYTDLLDNVDDLLARNIIIVPHGSFVEYMGTD
                     KFERMPIPTFGNRNVLRWESHRDKMREWLETAGIAMPRVIEDPKAIDRPVLVKYNGAK
                     GGRGFFIAKDYPEFKMGLDMEQEYTIQEYILGTRYYIHYFYSPLKKDGFRVGEGSLEL
                     MSMDRRDESNIDELYKLGASEELKKLGLFPTFVVTGNVPVVMRESLLPKVFDMGEQVV
                     KRSYELFGGMIGPFCLEGIVTDRLEFKVFEISSRIVAGSNPFISGSPYSDLVEPNMST
                     GRRISREIRNAVLTDSMDKIIS"
     gene            complement(1618..2130)
                     /locus_tag="PLJ11_05820"
     CDS             complement(1618..2130)
                     /locus_tag="PLJ11_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04224.1"
                     /translation="MDVIGALTSFLEQVQGDPLTYSVVFFIYIIAATVFLPVPVELML
                     FLSPATPFALKAVLLGVGKALGSVIVFYIGLNVEKPIVGLAKRWAFFRMLVSFCKWLV
                     DKLGYIGLYLILSVPVMVDTVPVYLFSIFKKERTDRDLRNFALTNLMAGVTRAAIVYY
                     VVTELGWDIL"
     gene            2259..2882
                     /locus_tag="PLJ11_05825"
     CDS             2259..2882
                     /locus_tag="PLJ11_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04225.1"
                     /translation="MVPEKKVGKAKDEDWFAKLDAELSARTDAISKDREEINFQKTTI
                     NRTIIGDFWNILNRFSRINVQLNMEPTYSEFAQFEEFPTKWKFKNEYDFEAVNKVQLV
                     DRTQTQGRMGDSLKVMYYAVDSTPYLRMVFDYCEGEHYYKYAGWKRIFGQFVIYDSPL
                     SKFDMDLFHEKLADVVLAWYESHLRNNRDILISHLKEKYERGETFTE"
     gene            3035..>3484
                     /locus_tag="PLJ11_05830"
     CDS             3035..>3484
                     /locus_tag="PLJ11_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04226.1"
                     /translation="MDREELAPHAEELARVLGEKVTKDRLLDELERYVNLYRVSLEWA
                     KRDILKKMGGDPGSIGNGSGRKSVAELTGAEQSVDLLVKVLTVNPKTIEVGGARKNIL
                     YGYVADESGRVPYTVWETESVQLKEGETVVIRNAYTKEYKGAPQLNLG"
BASE COUNT          735 a          976 c         1101 g          672 t
ORIGIN      
        1 accaagggcg agcctggaac ggggaacatc atcgaggccg tccggcatca gaggatcatt
       61 atggggaagg tgcgggagct gaagggcaag gacgaggcgg agctggcggt catcgcccgc
      121 cagatagagg cccccttcta cctcataaag gaggtggccc agctgcaacg gcttccggta
      181 atcaacttcg ccgcgggagg cgttgccacc ccggccgatg cggccctcat gatgcagcta
      241 ggctccgacg gagtgttcgt cggctccggc atcttcaaga gcgctgatcc ggccgtcagg
      301 gcgaaggcca tcgtggaggc cgtgacgcac tacgacgacc ccaaggcgat cgccgaggtg
      361 tccaaggggc tcggggaggc gatgcatggc atcgagatct ccaccttgcc caaggagcag
      421 cgcatgcagg agcgcggctg gtgatcgccc cccttcggct tggggcttgc taccagcccc
      481 ggggccttgg atccgacggc ccggtcagga gattatcttg tccatgctgt ccgtgagcac
      541 cgcgttcctt atctcccggg agatgcggcg cccggtgctc atgttcggct ccaccaggtc
      601 ggagtagggc gagccggaga tgaacgggtt ggagccggcc acgatcctcg aggagatctc
      661 gaagaccttg aactccagcc ggtcggtcac tatgccctcc aggcagaacg gcccgatcat
      721 cccgccgaac agctcgtagg agcgcttgac gacctgctcg cccatgtcga acaccttggg
      781 cagaaggctc tcccgcatga ccaccggcac gttgccggtc accacgaacg tcgggaacag
      841 gccgagcttc ttcagctcct cggacgcccc gagcttgtag agctcgtcga tattgctctc
      901 gtcccgcctg tccatggaca tgagctccag ggagccttcg ccgacccgga agccgtcctt
      961 cttgagaggg gagtagaagt agtggatgta gtacctggtg ccgaggatgt attcctggat
     1021 ggtgtactcc tgctccatgt ccaggcccat cttgaactcc ggatagtcct tggcgatgaa
     1081 gaagccgcgg ccgcccttcg ccccgttgta cttcaccagg accggccggt cgatggcctt
     1141 ggggtcctcg atgaccctgg gcatggcgat gcccgcggtc tcgagccact ccctcatctt
     1201 gtcccggtgg gattcccatc tcaggacgtt cctgttgccg aacgtgggga tgggcatcct
     1261 ctcgaacttg tcggtgccca tgtactccac gaaggagccg tgtgggacga ttattatgtt
     1321 tcgggccagt aggtcatcga cgttgtccag gagatcggtg tagctgtcca gcttgaggaa
     1381 ctcgtccggt cggcccaggg ggaaggcatc gtagtagcgg aagttgtcct tgacggtcag
     1441 ccccagcgtg cggaaccctt ccttcttggc cccgttgaag atctgaaggg aggaatggga
     1501 acagagggtg gccaccgtca gcttgctctt gtcgtactcg tcgagaacct tgaggatctt
     1561 gctcttcggg accattacct caaaattttc tttcgggata taaacatttc taacgaccta
     1621 aaggatgtcc catcccagtt cggtgaccac gtagtacacg atggccgccc tggtgacccc
     1681 cgccatcagg ttggtgaggg cgaagttgcg caggtcgcgg tcggtgcgtt ccttcttgaa
     1741 tatggagaac aggtacacgg gcacggtgtc caccatgacg ggcacgctga ggatcaggta
     1801 cagcccgatg taccccagct tgtccaccag ccacttgcag aacgagacca gcatgcggaa
     1861 gaaggcccac ctcttggcca ggccgactat gggcttctcc acgttcaggc cgatgtagaa
     1921 gacgatgacg cttccgaggg ccttgccgac ccccagcagc acggccttga gcgcgaaggg
     1981 ggtggcgggg ctcaggaaga gcatgagctc cacgggcacc ggcaggaaga ccgtggccgc
     2041 gatgatgtag atgaagaaga cgaccgagta ggtcaagggg tcgccctgca cctgctcaag
     2101 gaaggaggtg agggccccta tgacgtccat cgggcggact atgccctccc attagaaaag
     2161 cttatctcgc ttggcgcccg ccaggcgata atctgggtta ttatatgaat gagatatctt
     2221 tttatggttg tatggcaatt atgcgagagg agactccaat ggttccagaa aagaaggtgg
     2281 gaaaggcgaa ggacgaggat tggttcgcca agctggacgc cgagctttcg gcccgtaccg
     2341 atgccatctc caaggacagg gaggagatca acttccagaa gaccacgatc aacaggacca
     2401 tcatcggaga tttctggaac atcctgaaca ggttctccag gatcaacgtc caattgaaca
     2461 tggagcccac ttacagcgag ttcgcccagt tcgaggagtt ccccaccaag tggaagttca
     2521 agaacgaata cgacttcgag gcggtcaaca aggtccagct tgtagaccgc acccagaccc
     2581 aggggcgcat gggcgattcc ctgaaggtga tgtactacgc ggtggactcc accccctatc
     2641 tgcgtatggt gttcgattac tgcgaggggg agcattacta caagtacgcc gggtggaagc
     2701 ggatcttcgg gcagttcgtg atctacgact cccccctatc gaagttcgat atggacctgt
     2761 tccatgagaa gctcgccgat gtcgtccttg cgtggtatga gtcccatctg cgcaacaacc
     2821 gcgatatttt gatatcccat ctcaaggaga agtacgagcg cggggagacc ttcaccgagt
     2881 gagccatttt caacggcctt cgacggtcga tctccgccct ggcggcagcg gtatcgatgg
     2941 cattgtcccc gcattttcgg tccgccggga ataatgctat taaacgcaag gggcataatg
     3001 ggctgcctta ttagctaagg aatggaggca tgagatggac agagaggagc tcgcacccca
     3061 tgcggaggag cttgccaggg tgctcgggga aaaggttacc aaggacaggt tgttggacga
     3121 gcttgagagg tacgtgaacc tctatcgggt ctcgttggaa tgggccaaga gggacatact
     3181 taagaagatg ggaggggacc caggctcgat cgggaacggt tcagggcgca agagcgtggc
     3241 ggagctgaca ggcgccgaac agtccgtgga cctgctggtc aaggtgctca ccgtcaaccc
     3301 caagaccatc gaggtgggcg gggcgcgcaa gaacatcctg tacggatatg tggcggacga
     3361 gtcgggcagg gtcccataca cggtgtggga gacggagagc gtccagctca aggaagggga
     3421 gacggtcgtc atccgtaacg cctatacgaa ggagtacaag ggcgcgccgc agctgaacct
     3481 gggc
//
LOCUS       DAOSYN010000137         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_21571_length_3474_cov_5.167593, whole genome shotgun sequence.
ACCESSION   DAOSYN010000137 DAOSYN010000000
VERSION     DAOSYN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21571_length_3474_cov_5.167593"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /gene="gcvPA"
                     /locus_tag="PLJ11_05835"
     CDS             complement(<1..1083)
                     /gene="gcvPA"
                     /locus_tag="PLJ11_05835"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOO04227.1"
                     /translation="MPLDRRMLDELGIGSVEELFSDIPEEVRTDGIPLPPGKSEMEVV
                     RYMQELLSANLTAESAPCFLGGGVYHHFIPSAVGTIVSRSELATSYTPYQAEISQGML
                     QSLFEYQSYIAELTAMDAVNSSNYDWATALGEAATMCHRILPKRTFLVPEAMCWDKRS
                     VLGNYVSGAALNVVGYCYDPFTGGLDLDDLRSKMTSDVCGIYAEVPNLFGVIDPQVPK
                     LKQEFPDVPLVVGVNPLSLALVRPPGEYGADIVIGEGQPLGLPMNVGGPSLGLFACRM
                     EHVRKMPGRLIGMTRDREGRRAFCMTLQTREQHIRRSKATSNICTNEALLAVAVSVYL
                     GIVGGKGLRAIARLNMRNAAELMERVD"
     gene            complement(1161..1580)
                     /locus_tag="PLJ11_05840"
     CDS             complement(1161..1580)
                     /locus_tag="PLJ11_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Toprim subdomain protein"
                     /protein_id="HOO04228.1"
                     /translation="MTRGRSPRERLEEIQAVLEEMISRDPRTVILVEGMRDRGALTIL
                     GVGGEMVQVQSSDGVFGIAERLAKEGKSAMIMTDWDRKGGQLSRLLRSALEANAVPYD
                     DGLRQRLVIVARQDIKDVQSLPSLYSRLVQEVQARDR"
     gene            complement(1577..1873)
                     /locus_tag="PLJ11_05845"
     CDS             complement(1577..1873)
                     /locus_tag="PLJ11_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOO04229.1"
                     /translation="MDLSQCLRPSAEGSELDIIVTPHSAVPGVHGVDPWRRRLVVKVR
                     APPEGGRANEEVEALLASTIGSKVTVVRGHTQRMKVVHIQAPVGLVAKALGGDA"
     gene            1979..2665
                     /locus_tag="PLJ11_05850"
     CDS             1979..2665
                     /locus_tag="PLJ11_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04230.1"
                     /translation="MAPPLEPINYKEITEVHRKEKGSVDLVEVRRDLYPAFRGYLERL
                     KKESEEEIRKDPLSFKASSLTNEYKKVLTKGRQIFFYRMRKITAMATRASEGAKIDLG
                     RLTEEEREMYSQVLRSINECRDLALEGKAPEPPPMAPSGSVCATVDQGQLRQALAIVN
                     GKAPAAERPQATDRVLVRILEDLPEIAGIDRPYRLAKNDVVNLPAPIGCALIKRGKAQ
                     EIVPFWRRAE"
     gene            2715..2791
                     /locus_tag="PLJ11_05855"
     tRNA            2715..2791
                     /locus_tag="PLJ11_05855"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2749..2751,aa:Lys,seq:ctt)
     gene            3066..>3474
                     /locus_tag="PLJ11_05860"
     CDS             3066..>3474
                     /locus_tag="PLJ11_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO04231.1"
                     /translation="MHYIGLDIHKKDTQASVLDEKGKFIHGERIRTDVVDIGHFFERM
                     ERMNDDDRKVVIEATGFYFWIYDVIVERGHQVKVVHPNRAKALMKARSKPDRNDALML
                     AQLLRLGGLEGIYVPSEDVRELRDLTRHRESLVR"
BASE COUNT          684 a         1097 c         1062 g          631 t
ORIGIN      
        1 gtcgacccgc tccataagct ccgccgcgtt cctcatgttg aggcgggcga tcgccctcaa
       61 ccccttcccc ccgacgatcc ccaggtagac ggagacggcg acggccagca gcgcctcgtt
      121 cgtgcagatg ttggaggtgg ccttgctcct ccggatatgc tgctcccggg tctgcagggt
      181 catgcagaac gcccgccgtc cctcccggtc gcgggtcatc ccgatcagcc ggcccggcat
      241 cttccgcaca tgctccatgc ggcacgcgaa gagcccgagg gacgggccgc cgacgttcat
      301 cggtagcccc agaggctgcc cttcgccgat gacgatgtcc gccccgtact cgcccggcgg
      361 cctcaccagc gccagggaca gggggttgac ccccaccacc agcgggacgt ccgggaactc
      421 ctgtttcagc ttgggcacct gggggtctat cacgccgaag aggttcggca cctccgcgta
      481 gatgccgcag acatcggagg tcatcttgct cctaaggtca tcgaggtcca gccccccggt
      541 gaacggatcg tagcagtacc ccaccacgtt gagggccgcc ccggacacgt agttcccgag
      601 caccgatcgc ttgtcccagc acatcgcctc cggcaccagg aacgtgcgct tcggcaggat
      661 cctgtggcac attgtggccg cctcccccag cgccgtcgcc caatcgtagt tcgaggagtt
      721 caccgcgtcc atcgccgtca gctcggctat gtagctctgg tactcgaaca gggactgcag
      781 catgccctgg cttatctccg cctgataggg cgtgtaggag gtggcgagtt ccgagcgcga
      841 aacgatggtg ccgaccgccg aggggatgaa gtgatggtac acccctccgc ccaggaagca
      901 cggggcgctc tcggccgtca ggttcgcgga gaggagctcc tgcatatagc gcacgacctc
      961 catctcgctc ttgccagggg gaagcgggat gccatcggtc cggacctcct ccggaatgtc
     1021 ggagaacagc tcctccacgc tccctatccc cagctcgtcc agcatcctgc ggtccaatgg
     1081 catgctaccc ggccctccta gacccatatc ccaataaata gtttggctgg atgggaccgg
     1141 tccgcgttcg tggaagggcg tcagcggtcc ctggcttgca cttcctgaac gaggcgggag
     1201 taaagggatg gcagggactg cacgtccttg atgtcctgcc tggcgacgat gaccagcctt
     1261 tggcgcaggc catcgtcgta ggggaccgca ttggcctcga gcgcgctcct cagcagcctt
     1321 gagagctgcc ccccctttcg gtcccagtcc gtcatgatca tggcgctttt gccctccttc
     1381 gccaggcgct ccgcgatccc gaagacccca tcactgctct ggacctggac catctctcct
     1441 cccacaccca ggatcgtaag ggcaccccgg tcccgcatgc cctcgaccag aattacggtg
     1501 cgcggatcac gggagatcat ctcctccaga acggcctgga tctcctccag cctctcccgc
     1561 ggggagcgcc cccgggtcat gcgtcccctc cgagggcctt ggccacgagc cccaccgggg
     1621 cctggatgtg cacgaccttc atgcgctgcg tgtgaccccg gacgacggta accttggaac
     1681 cgatggtcga ggcgaggagg gcctcgacct cctcgttggc cctcccgccc tccggcgggg
     1741 cgcgcacctt caccacaagg cgccggcgcc atggatcgac gccatggacg cccggtacag
     1801 cgctgtgcgg ggtaacgata atgtccagct ccgatccttc cgctgagggc cgcaggcatt
     1861 gggagaggtc cacgcccttt gaacggcggg ggattactta atccttcagg ggccaacggg
     1921 actggcgctg ccacgacggg agggcacctt cttatatcaa caaccgaatc cttgagcgat
     1981 ggcgcccccg ctcgagccga tcaattacaa ggagatcacc gaggttcacc gcaaggagaa
     2041 gggctccgtc gacctggtgg aggtgaggcg tgacctctat ccggccttcc gggggtactt
     2101 ggaaaggctg aagaaggaga gcgaggagga gatcaggaag gaccccctct ccttcaaggc
     2161 cagcagcctt accaatgagt acaagaaggt cctgaccaag ggccgccaga tattcttcta
     2221 caggatgcgc aagataacgg cgatggccac ccgggcctcg gaaggggcca agatcgacct
     2281 tggcaggctg accgaggagg aacgggagat gtactcccag gttttgagat cgatcaacga
     2341 gtgccgggac ctggccctgg aggggaaggc acccgaacca ccgccaatgg cgccctccgg
     2401 ctcggtctgc gccacggtgg accagggcca attgaggcag gcgctggcca tcgtcaacgg
     2461 caaggccccg gccgctgagc ggccgcaggc gaccgacagg gtgctggtca ggatccttga
     2521 ggacctgccg gagatcgccg ggatcgatcg gccgtaccgc ttggcgaaga acgacgtggt
     2581 gaacctgcca gccccgatag ggtgcgcgct gataaaaagg ggcaaggcgc aggagatcgt
     2641 gcccttctgg cgcagggccg aataggccaa ttccgcaacg tttatctagg tcaagatgat
     2701 tgagtggctc actcgggctc gtagctcagc taggtagagc aatcggctct taaccgatgg
     2761 gccgagggtt cgaatccctc cgagcccgcc ataattctat ccaatgagtc gaatttcaag
     2821 gtcaattcgg ggtttttcgg gtcttgcagt ctggccggcg aaaccctttc gatccttttt
     2881 attaccttaa ccaggccagt ttagggaata tattggtgcc gagcttgcgg tcaagctcgg
     2941 tcgccccctt cggcacgtcg gagaacatcg cgcgttcgac ggtatcgacc ttgtacctga
     3001 tgatatgtta aaagaggtcc cccgctgatc tgggtaattg ccaaatagcc caaggcagga
     3061 gaaccatgca ctatatcgga ctggacatac ataaaaagga cacccaggct agcgtactgg
     3121 atgaaaaggg aaagttcatt cacggggaac ggataaggac cgatgtcgtg gacatcggtc
     3181 atttctttga gaggatggaa cgaatgaacg atgacgaccg aaaggtcgtc atcgaggcca
     3241 ctggcttcta tttctggatc tacgatgtaa tcgtggaacg gggccatcag gtcaaggtcg
     3301 tgcatccgaa ccgggcgaaa gcactgatga aagcgaggtc caaaccggac aggaacgacg
     3361 ccctcatgct cgcccaactg cttcggctgg gtggtttgga aggaatatac gtcccttccg
     3421 aggatgttcg ggaactgagg gacctcacaa ggcatcgaga gtccctggtc agaa
//
LOCUS       DAOSYN010000138         3412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_22017_length_3412_cov_3.859398, whole genome shotgun sequence.
ACCESSION   DAOSYN010000138 DAOSYN010000000
VERSION     DAOSYN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22017_length_3412_cov_3.859398"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3104)
                     /gene="ileS"
                     /locus_tag="PLJ11_05865"
     CDS             complement(<1..3104)
                     /gene="ileS"
                     /locus_tag="PLJ11_05865"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOO04232.1"
                     /translation="MIKQVQGNYDPNALEKRVQAYWAETDAYRKTKELRASSPEFYFV
                     DGPPYTTGYIHLGTAMNKTIKDMLIRFKRMHRLNVTDTPGYDMHGLPIEVKVERSIGV
                     KNKKEIEAYGIDRFVGTCKNFALEFQKQMNADFQELGVWMDWDRPYMTIAPGYIEAAW
                     WTLRRAYDKGLLIKANRVLPGCPRCETALAEAEIDYWDEMDPSIYVKFRLKDDPKVSL
                     LIWTTTPWTLPANLAVAAHPDFEYVKVRYRRIGITDTVIVMKGLEEDIRALGGWERFE
                     VLQTIQGDDLVGTEYITPFVDMVPYQTQAGGKWLHKVLPSKNVEASNTGLVHIAPGHG
                     PEDFELGKEFGLEPFCPVDETGTYTPLVGCKYVGMHIRKANPELLKDLEAMDALFYSG
                     TIEHRVGHCWRCKSPVLFRTTDQWFLRITQVKDQMLEEIEKVRWTPDWAGSSREYDWT
                     RNARDWCISRQRYWGIPIPVWTCKCGEMRVVGLVDELKDAEGYVEDMELHRPWIDGVK
                     LRCPKCGGKMVRVPDVLDVWFDSAVASWAQLGFPRQREAFERLWPAKLIVEAHDQTRG
                     WFYSQLAAGVIAFGRAPYESVLMHGWMLDGQGQPMSKSKGNVVEPGKVVKEHGADALR
                     FYLLKTSAPWEDIPFQMDGVKNARKTLNVLWNVVNFATTYMAIDSFDPQRVTRDMASP
                     HLNQEDAWLISRTEKLKNEFTRQVNALELHKAGRALEQYILDDLSRWYVRLIRDRMWS
                     EEGNAGKLAAYRTLYDAIMDTTLLLAPICPHLAEEVYQHMDGTKPSVHMMDWPASDLT
                     LVDERLEKAMALVQEIVETITRERQLRNVKLRWPMKRVVIKVSSNDMIQPLKALEGIL
                     RSQANVKAVEYVGAGEEWAETRLNVVPNPMAIGKVYRQWASKIAVLLQSRPATMIKES
                     MQRGEYSLGIEGQMVKIEPNMVSFTISLPKDVHSAPFSGGELYLDFEVTREIEAEGFA
                     REIIRRVQQTRKDMGLDVEEFIRVEVRADPRVAEYVKMWKPHIMTEVRAKALELVMEA
                     KGERV"
     gene            complement(3185..>3412)
                     /locus_tag="PLJ11_05870"
     CDS             complement(3185..>3412)
                     /locus_tag="PLJ11_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="FEN-1; Rad2; similar to eukaryotic enzymes;
                     endonuclease that cleave the 5'overhanging flap structure
                     that is generated by displacement synthesis when DNA
                     polymerase encounters the 5'end of a downstream Okazaki
                     fragment; as 5'endo-/exonuclease and
                     5'pseudo-Y-endonuclease activities; cleaves the junction
                     between single and double-stranded regions of flap DNA;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flap structure-specific endonuclease"
                     /protein_id="HOO04233.1"
                     /translation="ALQALGVDDPDMLEVRGIFLNFERTDDYKLVWKAPDRQGVLAML
                     CGEYGFSEKRVNGALDRFASGKKDQMRLDMF"
BASE COUNT          550 a         1153 c          990 g          719 t
ORIGIN      
        1 accctttccc ccttggcctc catcaccagc tcgagggcct tggcgcgcac ctccgtcatg
       61 atatgtggct tccacatctt gacgtactcc gcgaccctgg ggtcggcccg tacctccacc
      121 ctgatgaact cctccacatc cagccccatg tccttccgtg tctgctggac cctgcggatg
      181 atctcccggg cgaagccctc agcctcgatc tcccgtgtca cctcgaagtc caggtagagc
      241 tcgcctccgc tgaagggagc ggagtggacg tccttgggga gggagatggt gaaggacacc
      301 atattgggct cgatcttgac catctgcccc tctatcccca gggaatattc cccgcgctgc
      361 atgctctcct tgatcatggt ggctggacgg ctctgcagca gcacggcgat cttggaggcc
      421 cattgtctat acaccttgcc gatggccatc gggttgggga cgacgttgag cctggtctcc
      481 gcccattcct cccccgcgcc cacgtattcc accgccttga cgttggcctg ggagcgcagg
      541 atgccctcca gagccttcaa gggctggatc atgtcattgc tggagacctt gatcactacc
      601 cgcttcatgg gccagcggag cttgacgttc ctcagctggc gctcccgcgt gatcgtctcc
      661 acgatctcct gcaccagggc catcgccttc tccaggcgct catccacgag cgtgaggtcg
      721 ctggcaggcc agtccatcat gtggaccgag ggcttcgtgc cgtccatgtg ctggtacacc
      781 tcctcggcga ggtgggggca gatcggggca aggagcagcg tcgtgtccat gatcgcatcg
      841 tagagcgtgc ggtaggctgc cagcttgccc gcgttcccct cctcgctcca catgcggtcg
      901 cgaatgagcc gaacgtacca gcgcgaaaga tcgtccagaa tgtactgttc gagggcccgg
      961 ccagccttgt gcagttccag agcgttcacc tggcgggtga actcgttctt tagcttctcc
     1021 gtcctggata tcagccatgc gtcctcctgg ttcagatggg ggctggccat gtccctggtg
     1081 acgcgctgcg ggtcgaagct gtcgatggcc atgtaggtgg tggcgaagtt gaccacgttc
     1141 cagagcacgt tgagggtctt gcgggcgttc ttgaccccgt ccatctggaa ggggatgtcc
     1201 tcccagggcg cggaggtctt aaggagatag aagcgcagcg cgtccgcccc gtgctccttg
     1261 acgaccttgc ccggctcgac gacgttgccc ttgctcttcg acatcggctg cccctgacca
     1321 tccagcatcc agccgtgcat gagcaccgat tcgtagggcg cccggccgaa ggcgatcacc
     1381 cccgcggcca gctgggagta gaaccaaccg cgggtctggt catgggcctc gacgatgagc
     1441 ttggcgggcc agaggcgctc gaacgcctcc cgttgcctcg ggaaccctag ctgggcccag
     1501 gaggcgaccg ccgaatcgaa ccaaacgtcc aggacatccg gcacccggac catcttcccc
     1561 ccgcacttgg gacagcgcag ctttacgcca tcgatccagg gccggtggag ctccatgtcc
     1621 tccacgtacc cttcggcgtc cttcagctcg tctaccaagc cgaccaccct catctccccg
     1681 cacttgcagg tccagacggg gatcgggatc ccccagtacc gctgccttga tatgcaccaa
     1741 tcgcgggcgt tcctggtcca atcgtactca cgcgaggagc cggcccagtc cggcgtccac
     1801 cggaccttct cgatctcctc caacatctgg tccttgacct gggtgatccg caggaaccat
     1861 tggtcggtgg tacggaacag caccggggac ttgcaccgcc agcagtggcc tacgcgatgc
     1921 tcgatggtgc cgctgtagaa cagggcgtcc atcgcctcca ggtccttcag cagctctggg
     1981 ttggccttcc tgatgtgcat gccgacgtac ttgcagccca ccaggggcgt gtacgtcccg
     2041 gtctcgtcca ccgggcagaa gggctccagg ccgaactcct ttcccagctc gaagtcctcc
     2101 ggaccgtggc ccggggcgat gtgcaccagc ccggtattgg aggcctccac gttcttcgag
     2161 ggcaggacct tatggagcca tttgccgccc gcctgtgtct ggtaggggac catgtcgacg
     2221 aagggggtga tgtactccgt ccccactagg tcgtccccct ggatggtctg taggacctcg
     2281 aacctctccc agccccccag ggctctgata tcctcctcca gtcccttcat cacgatgacc
     2341 gtgtccgtga tcccgatgcg cctgtagcgc accttgacgt actcgaagtc cggatgggcc
     2401 gccaccgcga ggttcgcggg cagcgtccaa ggggtggtgg tccagatgag cagggagacc
     2461 ttagggtcgt ccttaaggcg gaacttgacg tagatggagg ggtccatctc gtcccagtag
     2521 tcgatctccg cctccgctag ggcggtctcg cacctcgggc agccggggag cacccggttg
     2581 gccttgatca gtagcccctt atcataggcg cgcctgaggg tccaccaggc cgcctcgatg
     2641 tacccggggg cgatggtcat gtacggccgg tcccagtcca tccatactcc cagctcctgg
     2701 aagtctgcgt tcatctgctt ctggaactcc agcgcgaagt tcttgcaggt cccgacgaac
     2761 cgatcgatgc cgtacgcctc tatctccttc ttgttcttga ccccgatcga cctctcgacc
     2821 ttgacctcga tgggaagccc atgcatgtcg tagccggggg tatcggtcac gttgagccgg
     2881 tgcatgcgct tgaaccggat cagcatgtcc ttgatggtct tgttcatcgc cgtcccgagg
     2941 tggatgtacc cggtcgtgta cggcgggccg tccacgaaat agaactcagg ggaggaggcc
     3001 cgcagttcct tggtcttcct gtacgcgtcc gtctccgccc aataggcctg caccctcttc
     3061 tctagcgcat tggggtcata gtttccttgg acctgcttta tcatcgccgt cccttaggtc
     3121 ccctatcggc catcaccctt ttaacctctt ggttctggct ggagcattgg ataacagcgc
     3181 cctgtcagaa catgtccagc cgcatctggt ccttcttccc tgatgcgaac ctgtcgagcg
     3241 ccccgttaac cctcttctcc gagaacccgt actccccgca gagcatggcc aagacccctt
     3301 gccggtccgg cgccttccat accagcttgt agtcgtcggt gcgctcgaag ttcaggaaga
     3361 tgccccgcac ctcgagcatg tccgggtcgt cgacgcccag ggcctgaagc gc
//
LOCUS       DAOSYN010000139         3371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_22322_length_3371_cov_4.762967, whole genome shotgun sequence.
ACCESSION   DAOSYN010000139 DAOSYN010000000
VERSION     DAOSYN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22322_length_3371_cov_4.762967"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..775
                     /locus_tag="PLJ11_05875"
     CDS             113..775
                     /locus_tag="PLJ11_05875"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO04234.1"
                     /translation="MRFDLHVHSTYSGDSINKVEEVLDICTRKGLAGVAIIDHNSLEG
                     FRHALSLGRHDVLIIPGMEVSSAEGHVLAYNIQEVIPRGLGVAETIDRIRAQGGMAVA
                     AHPYRMWSGLGEEATLANDFDAIEVHNGRSTHAHNDRAADLAVRMERPFTAGSDSHEP
                     STVGKAYFETSGECSTVEEVLKEVLSLKGRTGGTHRPRGDTLSYGVKCIGEWFGRGFK
                     KM"
     gene            complement(776..1123)
                     /locus_tag="PLJ11_05880"
     CDS             complement(776..1123)
                     /locus_tag="PLJ11_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOO04235.1"
                     /translation="MRGLEERDFESMLEKVTAIEGVVLAYICSRAGNYVKGSTPKFAD
                     RQMYSAITSLAFGTAEQVGHEMNDDLQYVSLRFSEHNLIVVGLGPRHLLGLLVDGRAD
                     PEHTLEQVKPLLA"
     gene            1229..2371
                     /locus_tag="PLJ11_05885"
     CDS             1229..2371
                     /locus_tag="PLJ11_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04236.1"
                     /translation="MPPVSLRSILPISAKARTEADRLEPVLVESLGSPLSMERKRMEG
                     RVLSAFEEEAGAIMAMLLRHMETRNDNAREAIDRLLNGLTASREGKAALLENLAHPDQ
                     EVRKGARTMLVRIWGEGMDAFATDYEQAMLLMNVARSRDIYVDDIMTLTELVKVTLLE
                     GDRERALEDIALIVKLLRHRYRSVETMKDYLAEMLRITPELSKLGVMSGRIEESLRTA
                     SRANRTRTFDYTKELIDERMREVELIDRMRSIGATVKEALTEAPHMPLDDISGTDMRM
                     FTHLKGLVEKGTTMSVTGRASEIVAMVSAFLADELFPYLEGKAQDRLSAMDPSLLYVI
                     YTVGLTCLKLMAEPLPSVSEELYVTYFKELEGAPSLAAVPWPSAVL"
     gene            2494..>3371
                     /gene="ftsZ"
                     /locus_tag="PLJ11_05890"
     CDS             2494..>3371
                     /gene="ftsZ"
                     /locus_tag="PLJ11_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO04237.1"
                     /translation="MVKNALANTKYANNTNGKAEPAKQEPVPAEPAPKSNIDEELEKI
                     AAQLDVCIKIIGCGGGGCNTINRCWEANINGAQLCAINTDAKHLLTVKAPRKILIGKT
                     ATRGLGAGAKPEMGEAAARESDSEIRDFLNGSNIVFVTAGLGGGTGTGAAHYVARIAK
                     EQVRALTIGVVTMPFKAEGTVRMENALDGLNKMRLICDTTIVIPNDKLLELVPKLPVD
                     AAFKVADEVLMQTIKGLTEIITKPGLVNLDYADIMTVMNEGGVAFVGIGEGNSDDDDR
                     VKAAVQEALTSPMLGE"
BASE COUNT          721 a         1026 c         1045 g          579 t
ORIGIN      
        1 aggccgcctt cttcctgccc agcgaaggcg cggccgtcgc cctgcccctg ttcaccgaaa
       61 gaaaggtcgc gatcggcgtg gacggtgagc tggcgaacgg ggcgaaagtg agatgaggtt
      121 cgacctgcac gtgcattcaa cgtactcggg cgattccatc aacaaggttg aggaggtcct
      181 ggacatctgc accaggaaag ggctggccgg ggtggcgatc atcgaccaca actccctgga
      241 gggcttccgc catgccctct cgctgggaag gcacgacgtc ctgataatcc cggggatgga
      301 ggtgtcctcc gccgaggggc atgtccttgc ctataacatt caggaggtga ttcctcgggg
      361 gcttggggtg gcggagacca tcgaccggat cagagcccag ggaggcatgg ctgtggccgc
      421 ccatccctat cgcatgtggt ccgggctcgg ggaggaggcc accctggcca atgacttcga
      481 tgcgatcgag gtccacaacg gacgttccac ccacgcccat aacgacaggg cggccgacct
      541 ggcggtcagg atggaaaggc cgttcaccgc tggcagcgac tcccatgagc cgtccacggt
      601 cggaaaggcc tacttcgaga cgtccggcga gtgctccacc gtggaggagg tcctcaagga
      661 ggtcctctcc ctgaaagggc gtacgggcgg cacgcacagg ccgagggggg acaccctgag
      721 ctatggcgtc aaatgcatag gcgaatggtt cggccgaggc ttcaagaaga tgtgatcatg
      781 ccaggagagg tttgacctgc tccaatgtat gttcagggtc ggccctgcca tcgaccagca
      841 gcccgagcag gtgcctaggg cctagcccaa cgactatcag attatgctcg gagaagcgaa
      901 gggacacgta ctgcaggtcg tcgttcatct catggccaac ctgctccgcg gtcccgaagg
      961 ccaatgaggt tatggccgag tacatctgtc ggtccgcgaa cttaggcgta ctccccttca
     1021 catagttgcc cgcgcgggag cagatgtagg ctagcacaac cccttcgatg gcggtcacct
     1081 tctccaacat cgattcgaaa tccctttcct ccaacccgcg catctcccga atgagatacg
     1141 gtaggggaaa ataaaacctt tactcgaaca gttgctggtc cctatctcag gacaaaagga
     1201 ttaatcggac ctcctccata ataccaccat gcctccggtc tcgctgcgca gcatcctccc
     1261 gatatctgcg aaggcaagga ccgaggccga caggctcgaa ccggtcctag tcgagtctct
     1321 gggctccccc ctctccatgg aacggaagcg catggaaggc cgtgttctca gcgcgttcga
     1381 agaggaagcc ggcgcgataa tggccatgct ccttcgccac atggagacga ggaacgacaa
     1441 tgcccgcgag gcgatcgacc gtcttctgaa cgggctgacg gcctcccgag aggggaaggc
     1501 cgccttgcta gaaaaccttg cccatccaga ccaggaggtg cgcaaggggg cccggaccat
     1561 gctggtccgg atctgggggg agggcatgga cgccttcgcc accgattatg agcaggcgat
     1621 gctgctgatg aacgtcgcgc gcagccggga catctacgtc gacgacatca tgaccctgac
     1681 cgagctggtg aaggtcaccc tgctcgaggg ggacagggag agggccttgg aggacatcgc
     1741 cctgatagtg aagctcctcc ggcatcgcta ccgctcagtg gagaccatga aggactatct
     1801 ggcggagatg ctgaggataa cccccgaact ttccaagctc ggggtcatga gcggaaggat
     1861 agaggaatcg ctgcgcacgg cctcccgagc caaccggacg cgcaccttcg attacaccaa
     1921 ggagctcatc gacgaacgga tgcgcgaagt ggagctcatc gatcgcatgc gctccatcgg
     1981 cgcaacggtg aaggaggcgc tgacggaggc gccccatatg ccgttggatg acattagtgg
     2041 cacggatatg cggatgttca cccacttgaa gggactggtg gagaagggca cgaccatgag
     2101 cgtgaccggc cgcgcgtccg agatcgtggc gatggtgagc gccttccttg cggacgaact
     2161 attcccctat ctcgagggaa aggcccagga caggctctcg gccatggacc cctccctttt
     2221 atacgtcatc tacaccgtcg gcctcacctg cctcaagctg atggccgagc cgctccccag
     2281 cgtctccgag gagctgtacg tgacatactt caaggagctg gaaggcgccc cgagccttgc
     2341 ggcggtccca tggccctcgg ccgtgctctg agcctttcat ccgggcgcat gtccgacatc
     2401 cgtcatccaa tgtccaggtc tttaaaattc agtaaaattt atatttgcga ttttccttac
     2461 ccttactaga gggatgcact tatgaccagt tctttggtta agaatgcttt agcgaacact
     2521 aaatatgcga acaacacgaa cggcaaggcc gaaccggcca agcaggaacc ggtccctgcc
     2581 gaaccagccc caaagtccaa catagatgag gagctggaga agatcgcggc ccagctcgac
     2641 gtatgcatca agatcatcgg ctgcgggggc ggtggatgca acacgatcaa ccgctgctgg
     2701 gaggccaaca tcaacggcgc gcagctatgc gccatcaaca ccgacgccaa gcacctgctg
     2761 accgtcaagg ctcctcgcaa gatcctcatc ggaaagaccg ccacccgcgg cctgggggcc
     2821 ggggccaagc cggagatggg cgaggccgct gcccgcgaga gcgacagcga gataagggac
     2881 ttcctgaacg gctcgaacat tgtgttcgtc accgccggcc tgggcggtgg gaccggcacg
     2941 ggcgcggccc actatgtcgc ccggatcgcc aaggagcagg tgcgcgcgct cacgatcggc
     3001 gtggtcacca tgcccttcaa ggccgagggc accgtgcgca tggagaacgc cctggacggc
     3061 ctcaacaaga tgaggctcat ctgcgacacc accatcgtca tcccgaacga caagctcctg
     3121 gagctggtcc ccaagctgcc ggtcgatgcc gccttcaagg tggccgacga ggtgctcatg
     3181 cagaccataa aggggctgac ggagatcatc accaagcccg ggctggtcaa cttggattac
     3241 gccgacatca tgaccgtgat gaacgagggc ggcgtggcct tcgtcggcat cggggagggc
     3301 aacagcgatg acgacgaccg cgtgaaggcg gcggtgcagg aggcgctcac ctcccccatg
     3361 ctcggtgaga t
//
LOCUS       DAOSYN010000140         3340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_22591_length_3340_cov_5.312024, whole genome shotgun sequence.
ACCESSION   DAOSYN010000140 DAOSYN010000000
VERSION     DAOSYN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22591_length_3340_cov_5.312024"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1058)
                     /locus_tag="PLJ11_05895"
     CDS             complement(48..1058)
                     /locus_tag="PLJ11_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO04238.1"
                     /translation="MEDGAQGDVYARRYRILAAIMLGGIMGPIDASIVNVTLPTIAQH
                     YGAELSTAQWVPMVYLLVISSLLLLFGRLGDIIGYKKVYLAGLGGFTLASAMCSATYF
                     MPTIHWLIAFRAVQGLTASMLMAVQIAVITASFPSTERGKALGINAISISAGLAIGPS
                     LGGFLTHSFGWPSVFLVNVPIGIVGLLWAHHILPELKGRSGAVDWLGATASFVSLLSF
                     LLFVNSVQRSGFEPAALAMLLVAVVAAALFLRTEKKAPSPLVDLGLFRQRTINTVLPD
                     HRDSQRRIAWVDVFLCPLSLAAAFYGLAKSAVSRKMLWRGKIYELRSPNETVVTEVTS
                     TL"
     gene            complement(1642..2445)
                     /locus_tag="PLJ11_05900"
     CDS             complement(1642..2445)
                     /locus_tag="PLJ11_05900"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOO04239.1"
                     /translation="MTVPLYDVQETNVAIEALRTISRSLPEPFLLIGGWAVYLTVNES
                     FSKIHGTSYLGSRDIDICFHVDPGQDIDTLRGSTFARALEVVKKEGYAPHGSYRFCKM
                     IRKADGTILTEEMAKGFPLYEIIYLYIDMMVDNIHPQHKNIFKCDPLDEPIAAQIFMD
                     GSYVKKKLGDFYVNIPNPASLLATKLRSIPQRQKDDKLWKDACDIYSIIWHSPEKYSS
                     IVKKVKEEYPDDCAKARIAITKDVANRAAYHIGIERDEFIGVIDLLKLL"
     gene            complement(2442..3059)
                     /locus_tag="PLJ11_05905"
     CDS             complement(2442..3059)
                     /locus_tag="PLJ11_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04240.1"
                     /translation="MTIRKGGTEFSHYIQEGDKVERIYRVLLSIRGIGRSWYRIAKDA
                     DVSYGWAYSILKGLERDGLIDGHKVIQPKALFMRWVERKDLRLFREYNVQDLDDVIAK
                     SELDHVFTGYFAENLIGRYLFPRYREFYVKKEDALKWHQLLTGQGYVGRGNAQIILAD
                     DHVFFERQCIDKHPLVSTQQLIVDLYRTGAECAEAADLLVSKVYQ"
BASE COUNT          789 a          873 c          895 g          783 t
ORIGIN      
        1 cgggcggggc cgggccgcag atgcgtccgc caaggccggc gagcggttca gagtgtgctc
       61 gtcacttccg tcaccaccgt ttcgttcggg ctgcgaagct cgtatatctt gccccgccag
      121 agcatcttgc ggctgacggc ggacttggct aggccgtaga acgccgccgc cagcgagagc
      181 gggcagagga agacatcgac ccacgcgatg cggcgctgcg agtcgcggtg gtccggcaga
      241 acagtgttga tcgtccgctg gcggaagagg cctaggtcga cgagcgggga tggcgccttt
      301 ttttcggtcc tcaggaagag ggcggcggcg accaccgcga ccagcagcat ggcgagggcc
      361 gcgggctcga accccgatct ctggacggag ttgacgaaga gcaggaagga taggagggag
      421 acgaacgagg cggtcgcgcc gagccagtcc acagcgccgc tccgtccttt cagctcgggg
      481 aggatgtggt gggcccacag cagcccgacg atcccgatgg ggacgttcac gaggaacacc
      541 gagggccaac cgaaggagtg ggtcaagaac ccgccgaggg aggggccgat ggccaggccg
      601 gccgagatgc ttatggcgtt gatgcccagc gccttgcccc tctccgtcga ggggaacgag
      661 gcggtgatga cggcgatctg cacggccatc agcatgctgg ctgtgagccc ctggaccgcc
      721 ctgaaggcga tgagccaatg gatcgttggc ataaagtacg tggcgctgca catggccgag
      781 gcgagcgtga agcctcccag cccggcaagg tataccttct tgtacccgat gatgtcgccc
      841 agccggccga ataggagaag caggctgctt atgacgagga gatagaccat cgggacccat
      901 tgggccgtcg acagctccgc gccatagtgc tgcgctatcg tgggcagcgt gacgttcact
      961 atgctggcgt cgatcggccc cattatgccg ccgagcatga tagcggccag gatccgatat
     1021 ctgcgtgcgt atacgtcgcc ctgggccccg tcctccacgc ccgatcatcc cccgaggcgg
     1081 tacgggcggc tggcatcatt gggggaaacg ggccgatcac gctacggtcc tgctagattc
     1141 cataggcctt ggcctggctc attgcacccc gagaggccgt gccgaccgtt cgcccggtcc
     1201 gggtgtgggc acgaccgcgc caactggaga tgccctccgt cgctatacct ttcatcagca
     1261 tccgtcatgg cgtacttaat gatatttcga tgattggccc tatgggaatg atttaattga
     1321 agaatatgta ataaattcaa tgagcatcaa cacaatttgg tttcgcataa ggttggaaat
     1381 gcccttgcca agatagtctg atgctgcagg cgcgcggacc gtagaaggtc cggatcgccc
     1441 tcacaccttg gtccggcgat ggtcggtcag tggacccgtg tcattatgaa gcttgctgtc
     1501 cgaagaagat gcatggtggg ctcagccgag actgcgtgtc actcaagcca gacaatgaaa
     1561 aattgaaatc aattatcaga cagttaacgc aggccagtcg ttcaaaccct tttaccttat
     1621 tatagtaagt tcttactttg ctcacaacaa cttaagaagg tctatgaccc ctatgaactc
     1681 atctctctcg atcccaatat gataagcagc ccgattcgca acatcctttg ttatggcaat
     1741 gcgagccttc gcacaatcgt cagggtattc ttctttgacc tttttgacga ttgacgaata
     1801 tttctctggc gaatgccaga tgattgaata tatgtcgcaa gcgtccttcc aaagcttatc
     1861 atccttttga cgttgcggaa tcgaccttag ctttgttgcg agcaaggagg cgggatttgg
     1921 gatgttaacg taaaaatctc ccaatttttt ctttacgtaa ctaccatcca tgaatatctg
     1981 ggctgcaatt ggctcatcga gcgggtcgca tttgaaaata tttttatgct gtggatgtat
     2041 attatccacc atcatatcga tgtataggta tatgatctca taaagtggga atccttttgc
     2101 catctcctcg gtcaggatcg ttccgtcggc cttgcgaatc atcttacaaa accggtagga
     2161 gccatgaggc gcgtaaccct cctttttcac aacttctaaa gcccgggcaa atgtgctacc
     2221 acgaagagtg tcgatgtctt gtcctgggtc gacatgaaag cagatgtcga tatccctgga
     2281 acctagatat gatgtcccat gaatcttgct gaatgactcg ttgacggtga gataaacagc
     2341 ccatccacct atgagcagga atggttctgg tagcgaacgg gagatcgtcc taagcgcctc
     2401 tatcgcaaca tttgtctcct gaacatcgta gagtggcact gtcattgata caccttagac
     2461 actaacagat cagccgcctc tgcacactcg gccccagtgc gatataggtc gacgatcagt
     2521 tgttgcgtgg ataccaaagg atgtttgtct atgcattgtc tctcgaagaa cacgtgatcg
     2581 tcggcaagaa tgatctgcgc gttccccctg cccacatagc cttgcccggt tagaagctga
     2641 tgccatttca gtgcatcttc cttcttaaca tagaactcgc ggtaacgggg aaacaggtac
     2701 cgaccgataa ggttctcggc aaagtaaccg gtgaatacgt ggtcaagttc tgatttggca
     2761 atgacatcgt ccaaatcctg aacgttgtat tctcggaata gtctgaggtc cttccgttcc
     2821 acccacctca tgaacaacgc cttgggttgg attactttgt gtccatcgat caatccatcg
     2881 cgttcaagac cttttagaat actatacgcc cagccatagc ttacatccgc atcctttgcg
     2941 atgcggtacc atgatcggcc gatccctcgt atgcttagaa ggacacgata aatgcgttca
     3001 accttgtctc cctcctgaat gtaatgtgag aactcggtgc cgcccttgcg aatcgtcacg
     3061 atatatctta gtatgtaatc tatattaata gatatcggat atatcttaca gtatatgttg
     3121 tatttaaaat caccggataa cgacacttac atacgattga ctgaacagaa agatactggc
     3181 ttaagaaaat tgaaagttcc tggcaagagc gcctgaatac taaggataaa tgcgtctgat
     3241 tgggagcttt gcaataaaaa tatcaaatgg aatggaccaa atagaccacg catcgattga
     3301 tccagatgat taaaaagtaa atttcaatcg atcggtatcg
//
LOCUS       DAOSYN010000141         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_22775_length_3317_cov_3.970264, whole genome shotgun sequence.
ACCESSION   DAOSYN010000141 DAOSYN010000000
VERSION     DAOSYN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22775_length_3317_cov_3.970264"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..482
                     /gene="rpl18a"
                     /locus_tag="PLJ11_05910"
     CDS             237..482
                     /gene="rpl18a"
                     /locus_tag="PLJ11_05910"
                     /inference="COORDINATES: protein motif:HMM:NF001981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HOO04241.1"
                     /translation="MKAFQVIGSYDASRVGYRKSIQRFNVQVAAADEAAAKEKVLSDL
                     GSKHRKKRPEITFTEIRPLSPSEVTDPVVKHQIGEGR"
     gene            479..904
                     /gene="pfdA"
                     /locus_tag="PLJ11_05915"
     CDS             479..904
                     /gene="pfdA"
                     /locus_tag="PLJ11_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HOO04242.1"
                     /translation="MNEQELRQAMSAIEAYRAQLEAVNEQAQLLQMSLEDYSRAKDTL
                     EAVAKGRSGEEVLMPIGGGAFVYAAIAKTDRALVGVGSGVSIDRPMEEAIAAVNARIG
                     ELMDALKKVGESGTVLEAKIEQLTQAVEQEYQRMRRDQK"
     gene            912..1853
                     /gene="ftsY"
                     /locus_tag="PLJ11_05920"
     CDS             912..1853
                     /gene="ftsY"
                     /locus_tag="PLJ11_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOO04243.1"
                     /translation="MFESLKKLLGLSKKEIEPTAKVEEVVGDSGKRISERDLDEFLWD
                     LELVLLEADVALPIVEEIKASVRADLLGKRVDRSYKVEDAIELALKNAVRAVLKGSEF
                     DFDAFVATHERPVVIMFLGINGTGKTTVIAKIAHRLQGQGLSVVLSASDTFRAGAIEQ
                     LAVHGERLGAKVVRHQAGGDPAAVAYDAVEHAKARKRDVVLVDTAGRMQTNANLMDEM
                     KKIKRVVRPHLTVFVGDSLAGNDAIEQARAFEREIGIDAVILTKIDADAKGGAALSIA
                     HSIKKPIAFLSTGQGYEDLIRFDSQWMVDRLFGNGRE"
     gene            complement(1857..3107)
                     /locus_tag="PLJ11_05925"
     CDS             complement(1857..3107)
                     /locus_tag="PLJ11_05925"
                     /inference="COORDINATES: protein motif:HMM:NF010589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOO04244.1"
                     /translation="MPSLEQLLAQVGSHREEMVEALSELIRIPAIGPESGGSGELERS
                     RYLRDILDHSKFDDVEMYDALDERVRLRLRPNIIARRRGKADQTVWIVAHMDTVPPGD
                     LSAWSTPPFSPRLVDGKLYGLGAEDNGQAIISGLFAAETLMSTGVELERGLGIALVAD
                     EEAGNEKGIRFLLQEGVFKPGDIIFVPDHGTPRGDEVEIAEKHILWLKFTVTGRQVHA
                     SRPDLGINAMRVGSELIVFLQEFLQRKYGERDPLFTPDRSTFEPTKRLQTVGNVNTIP
                     GEDVFYFDCRVLPRYDLDAVQSDITAITDIFEQKTGARIRVEPVQLTRSGRPSDPEGE
                     GMRALRWAVQRVLGVEPRPVGIGGGTCANFFRLAGMDAYVWQTIEELAHQPNEYCVID
                     NLVNDAKVYATVLAALCCGSQFKA"
     gene            complement(3159..3305)
                     /locus_tag="PLJ11_05930"
     CDS             complement(3159..3305)
                     /locus_tag="PLJ11_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04245.1"
                     /translation="MALGHPIGCSGARVLTSLVYALKDRGKRTGLATLCLGGGNAVSM
                     VVRA"
BASE COUNT          590 a         1057 c         1132 g          538 t
ORIGIN      
        1 gaccatcccc cgcaggaagc gccctttgca gtgctcgcat aggagggcgc acccggcccc
       61 cgtcactgag acggagggga accgctttcc gggacggtag acggcgatgg tcctcatggt
      121 acggggatcg atggcgcccg agggcttcgt tctattggtc ggtggataac acacgcggag
      181 cggagggacc aaaagcttta ttgggcgaag cccgttcctc gcccgaggca atcccgatga
      241 aggctttcca ggtgatcggc tcctacgatg cgagcagggt tggctaccgc aagagcatcc
      301 agaggttcaa tgtacaggtg gcggccgccg atgaggccgc ggcgaaggag aaggtgctgt
      361 ccgacctggg cagcaagcac cgcaagaagc ggcccgagat aaccttcacc gagataaggc
      421 ccttgagccc gtccgaggtg accgacccgg tcgtgaagca ccagatcggt gaggggagat
      481 gaacgagcag gagctccgcc aggccatgag cgccatcgag gcctacaggg cccagctcga
      541 ggccgtcaac gagcaggccc agctcctgca gatgtccctg gaggactatt cccgggccaa
      601 ggacacgctg gaggccgtgg ccaagggacg gtcgggggag gaggtgctca tgccgatagg
      661 cgggggcgcc ttcgtctacg cggccatagc gaagaccgac cgggcgctgg taggcgtggg
      721 cagcggcgtt tccatcgacc ggccgatgga ggaggccatc gccgcggtga acgcacgcat
      781 cggggagctg atggatgccc tgaagaaggt gggggagagc ggcaccgtcc tggaggccaa
      841 gatcgagcag ctcacccagg ccgtcgagca ggagtaccag aggatgcgca gggaccagaa
      901 gtgagcgggc catgttcgag tccctgaaga agctcctggg cctgagcaag aaggagatcg
      961 agcccaccgc caaggtggag gaggtcgtcg gcgattccgg gaaacggatc tcggagcggg
     1021 acctggacga gttcctctgg gatctggagc tcgtgctgtt ggaggcggac gtcgccctgc
     1081 ccatcgtgga ggagatcaag gcgtccgtcc gggcggacct cctgggaaag cgggtcgatc
     1141 gctcctacaa ggtggaggac gcgatcgagc tcgccctcaa gaacgccgtg cgcgccgtgc
     1201 tgaagggcag cgagttcgat ttcgacgcct tcgtcgccac gcatgagcgg ccggtcgtca
     1261 tcatgttcct ggggatcaac gggacgggca agacgacggt catagctaag atcgcccatc
     1321 ggctgcaggg gcaggggctg agcgtcgtgc tctccgcctc cgacacgttc agggcggggg
     1381 ccatcgaaca gcttgcggtg cacggcgaac ggctcggcgc caaggtcgtg cggcaccagg
     1441 cgggcgggga cccggcggcc gtggcgtacg atgcggtgga gcacgccaag gcccggaaga
     1501 gggacgtggt gctcgtggac accgcggggc gcatgcagac caacgccaac ctgatggacg
     1561 agatgaagaa gatcaagcgc gtggtgagac cacatctcac ggtcttcgtg ggggattccc
     1621 tggccggcaa cgacgcgatc gagcaggcga gggcgttcga gcgggagatc ggcatcgacg
     1681 cggtcatcct gacgaagatc gacgccgacg ccaagggcgg ggcggcgctc tccatcgcgc
     1741 actccatcaa gaagccgata gccttcctgt ccaccgggca ggggtacgag gacctgataa
     1801 ggttcgacag tcagtggatg gtggaccggc tgttcggcaa tgggcgcgaa tgagcctcag
     1861 gccttgaact ggctgccgca gcagagcgcc gccagcaccg tggcgtacac cttggcgtcg
     1921 ttcaccaggt tgtcgatgac gcagtactcg ttcggctggt gcgccagctc ctctatcgtc
     1981 tgccagacgt aggcgtccat gcctgcgagg cggaagaagt tggcgcacgt cccacccccg
     2041 atgcccaccg gcctgggctc caccccgagc acgcgctgca ccgcccacct cagcgccctc
     2101 atcccctccc cctccgggtc cgaggggcgg ccgctcctcg tcagctgcac cggctccacc
     2161 cttatcctgg cgccggtctt ctgctcgaag atgtcggtga tggcggtgat gtcgctttgg
     2221 accgcgtcca gatcgtagcg gggcaggacc ctgcagtcaa agtagaacac gtcctccccg
     2281 gggatcgtgt tgacgttccc caccgtctgc agccgcttgg tcggctcgaa cgtcgaccgg
     2341 tcgggggtga acagcgggtc ccgctccccg tacttccgct gcaggaactc ctgcaggaag
     2401 acgatgagct cagagccgac ccgcatggcg ttgatgccca ggtccgggcg ggaggcgtgc
     2461 acctgccttc cggtcacggt gaacttgagc cagaggatgt gcttctccgc tatctccacc
     2521 tcgtcccccc gcggggtccc gtggtccggg acgaagatga tgtcccccgg cttgaagacg
     2581 ccctcctgca ggaggaaccg gatgcccttc tcgttccccg cctcctcgtc cgcaacgagg
     2641 gcgatgccca gccccctctc caattccaca cccgtgctca tcagggtctc ggcggcgaag
     2701 aggccggaga tgatggcctg cccgttgtcc tccgccccca gcccgtagag cttgccgtcg
     2761 accaatcggg gcgagaaggg cggcgtgctc caggcgctca ggtcccctgg gggaacggtg
     2821 tccatgtgcg ccactatcca gaccgtctgg tccgccttcc ctctcctccg ggcgatgatg
     2881 ttaggcctca gcctcagccg gaccctctca tcgagggcgt cgtacatctc cacgtcgtcg
     2941 aacttggaat ggtccaggat gtcccttaga tagcgggacc tctccagctc cccgctcccc
     3001 ccgctctccg ggccgatcgc agggatccgt atgagttcgc tgagggcctc gaccatctcc
     3061 tcccggtggg acccgacttg ggccagtagc tgttccaggc tgggcattga aatcgataat
     3121 gattgcgatt gcccatataa gatttatttg gaggcggcct aggcgcggac caccatcgag
     3181 acggcgttcc ctccgccgag gcacagagtg gccaggccgg tgcgcttgcc ccggtccttc
     3241 agggcgtaga ccagggaggt gagcaccctg gcgccggagc agccgatcgg gtggccgagg
     3301 gccaccgccc cgccgtt
//
LOCUS       DAOSYN010000142         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_22776_length_3317_cov_3.860515, whole genome shotgun sequence.
ACCESSION   DAOSYN010000142 DAOSYN010000000
VERSION     DAOSYN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22776_length_3317_cov_3.860515"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /gene="gvpD"
                     /locus_tag="PLJ11_05935"
     CDS             complement(<1..868)
                     /gene="gvpD"
                     /locus_tag="PLJ11_05935"
                     /inference="COORDINATES: protein motif:HMM:NF018756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD"
                     /protein_id="HOO04246.1"
                     /translation="MSHPNVGILPQEIIDFLLSPGGHSLLVKGEAGTGKTTMALQIIE
                     ALSDQQPEYYLSSRVSDIALYHQFPWLKERVQRNQLMRAGMAFLRRSWAMEGARRELG
                     EAEPQVDRRELNRLEGQIEAGELGLEEEEGEVVHPDGSITVEIGSVLPELEMAYDIVE
                     SNLPRKTLVVLDSVEALAESYGIPAFRIVSAVQKDLVEKADTNAVFVMETADHSHLDY
                     LGDGIVVLRNPVQGNDRVRTMEIEKLRGSSIRNWRYLFTLEGGRVTVMGRDLALGDVR
                     LTRGDGLPLPGRG"
     gene            975..2354
                     /gene="purB"
                     /locus_tag="PLJ11_05940"
     CDS             975..2354
                     /gene="purB"
                     /locus_tag="PLJ11_05940"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOO04247.1"
                     /translation="MKPYYARCVSMLTCPLDFRYGRKEMKAIFSEDHRIDLQLKVEAA
                     LARAHAKVGTIPRSAAEEITAKASIAIVPRERVSEIEAETKHDVMAMVKALSERCGDS
                     GRYVHLGATSNDIIDTTTALQLKAALDIIEADLLALLSTFADLAERHRTTICMGRTHG
                     QNAIPTTYGFKVAGYASEVMRHLERLREGRRRVCVGKLSGAIGTAAALGPKARQVQAH
                     MMKDLGLGYEEAATQVVCRDRYAELVCLMAIICTSCERYATEVRNLQRSEIAEVAEPF
                     DTKRQVGSSTMAQKRNPIVSENVCGLSRIVRGFVPPTFENMVLWHERDLTNSSAERFT
                     LPHVMILTDDILVKMEGVYRGLTVNEGNMLRNIKAAKGMIMAEPVMMALTSKGLGRQE
                     AHEIVRSTSMLAEEKGWDLERALWRNPVVRKHLTKEELASVMDPANYLGSAPEMTDAV
                     VAKARKLLQ"
     gene            complement(2391..3011)
                     /locus_tag="PLJ11_05945"
     CDS             complement(2391..3011)
                     /locus_tag="PLJ11_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOO04248.1"
                     /translation="MTIEEIDLYWLKDDDLRRYLPGKDCGECGTPDCAGFANALLMGK
                     RKAGDCPYLDRKMAEVMDSICALDIHLGESDPMLRKVPDPLLEINGPDASSPVILTSS
                     SVITIDILRQVLTAAGSKCFIVPMDTKGYTVDNSVHEGTITQMAIMKAMAGSGIGERV
                     SHRTLLIPGLAAELKGLVERIARWTVKVGPVSGFELPLFLLTEWTE"
     gene            3224..3317
                     /locus_tag="PLJ11_05950"
     tRNA            join(3224..3242,3265..3317)
                     /locus_tag="PLJ11_05950"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3278..3280,aa:Ala,seq:cgc)
BASE COUNT          653 a         1033 c         1016 g          615 t
ORIGIN      
        1 agccccgccc cgggagggga aggccatcgc ccctggtcag acgcacatcc cccaaggcaa
       61 ggtccctccc catcaccgtc acccttccgc cttccagggt gaagaggtac ctccagttcc
      121 ggatggatga gcccctgagc ttctcgatct ccatggtgcg cacgcgatcg ttcccctgga
      181 cgggattgcg cagcaccact atgccatccc cgaggtagtc caaatggctg tggtcggcgg
      241 tctccatgac gaagacggcg ttggtgtcgg ccttttccac caggtccttc tgcaccgcgc
      301 tgacgatcct gaaggcaggg atgccatagc tctctgccag cgcctccacc gagtctagga
      361 ccacgagggt cttccgcggg aggttgctct ccacgatgtc ataggccatc tccagctcgg
      421 gaaggaccga gcctatctcc accgtgatcg agccgtccgg gtggaccacc tccccctcct
      481 cttcctccag gccgagttcg cccgcctcga tctgtccttc cagacgattg agctcccggc
      541 ggtccacctg tggctcggcc tcgcccagct cccttcgcgc cccttccatt gcccaggagc
      601 gccggaggaa ggccatgccc gccctcatca gctggttccg ctgcaccctt tccttcaacc
      661 aggggaactg atggtagagg gcgatgtccg agacgcggga ggaaaggtag tactcaggct
      721 gctggtccga cagcgcttcg atgatctgca gggccatggt cgtcttgccg gtgccggcct
      781 cccccttgac caggagggaa tgccctccag gtgagagcag gaagtcgatg atctcctggg
      841 gcaggatccc tacgttagga tggctcacgg aacatcaggc gaatataccc cggtccgcca
      901 tattaattat ggtgcctgat tatcgatcgg ggcgagcggg gacgctagta agcttaaatg
      961 ccaaaagcat catcatgaag ccatactatg cgcggtgcgt ttcaatgctt acatgtccct
     1021 tagacttccg ctacgggcgc aaggagatga aggccatctt ctccgaggac cacaggatag
     1081 acctccaatt gaaggtggag gcggccctgg cccgagccca cgccaaggtc ggcaccatcc
     1141 cccgctcggc cgccgaggag atcaccgcca aagcctccat cgccatcgtc ccgcgcgaaa
     1201 gggtgagcga gatcgaggcg gagacgaagc atgacgtaat ggccatggtc aaggccctgt
     1261 cggagaggtg cggcgactcc ggaaggtacg tccatctggg ggcgacctcc aacgacatca
     1321 tcgacacgac caccgccttg cagctgaagg ccgcgctgga catcatcgag gcggacctgc
     1381 tggcgctcct ctcgacgttc gccgacctgg ccgagaggca ccggacgacg atctgcatgg
     1441 gccgtaccca tggccagaac gccatcccta ccacctacgg gttcaaggtg gctggctacg
     1501 cgtccgaggt catgcggcac cttgagcgcc tccgggaggg gcggaggagg gtgtgcgtcg
     1561 gcaagctctc cggggcgata gggaccgcgg cggcgctggg ccccaaggcc aggcaggtgc
     1621 aggcgcacat gatgaaggac ctcgggctgg gctacgaaga ggccgcgacc caggtcgtgt
     1681 gccgggaccg atacgccgaa ctggtatgcc tgatggccat catctgcacc tcgtgcgaga
     1741 ggtacgccac cgaggtgcgc aatctgcagc gctccgagat cgcggaggtg gccgagccct
     1801 tcgataccaa gagacaggtg ggaagctcga ccatggccca gaagcgcaac cccatcgttt
     1861 cggagaacgt ttgcgggctg agccgcatcg tgcgcggctt cgtccctccc acgttcgaga
     1921 acatggtgct gtggcacgag agggacctca ccaattcctc ggccgagcgc ttcaccctcc
     1981 cgcacgtgat gatattgacc gacgacatcc tggtgaagat ggagggcgtc taccgagggc
     2041 tgaccgtcaa cgagggcaat atgcttagga atatcaaggc ggcgaagggg atgatcatgg
     2101 ccgaacctgt gatgatggcc ttgacctcga agggcctggg aaggcaggag gctcatgaga
     2161 tcgttcgctc gaccagcatg ctggccgagg agaaggggtg ggacctcgaa agggccctgt
     2221 ggcggaaccc ggtggtgagg aagcacctca ccaaggagga attggcgtcc gtcatggacc
     2281 ctgccaatta cctagggtcc gcgccagaga tgacggacgc cgtagtggca aaggcgcgaa
     2341 agctcctcca gtagcctatt tcgggggccc cgggaacgaa acgctccggg tcattcggtc
     2401 cattccgtca aaaggaacag gggtagctcg aagccggaca ccggtccgac cttcaccgtc
     2461 catctcgcta tcctttcgac aaggccctta agctccgccg caagcccggg gatcagcagc
     2521 gtgcggtgcg agaccctctc ccctatgccg ctcccggcca tggccttcat aatagccatc
     2581 tgggtgatgg tgccctcatg gacggagtta tccactgtgt agcccttggt gtccatgggg
     2641 actatgaagc acttgcttcc cgccgcggtg aggacctgcc tgaggatgtc gatggtgatc
     2701 actgagctgg aggtgaggat cacgggcgag gaggcgtcag ggccgttgat ctccaataat
     2761 gggtccggga ccttccggag catcggatcg ctctccccta aatggatgtc caacgcgcaa
     2821 atgctgtcca tgacctctgc cattttcctg tccaaatatg gacaatcccc tgccttcctc
     2881 tttcccataa ggagggcgtt cgcgaagccg gcgcagtcag gggttccaca ttccccgcag
     2941 tcctttccgg gaaggtacct tcggaggtca tcgtccttca gccagtagag gtcgatctcc
     3001 tcaatggtca tggcatcacc agaattcgaa gtacgccctg acataaataa taatatccat
     3061 gcgaacgtcc ttgatgatct tgggccttgg tagttcgggc cggctcccat accctgtcct
     3121 gggaccggaa cgataggtcg agcggggaag ggggatggcc ccgtaggggc gttctccacc
     3181 tacaaaggat tataaacgtg ggtacgattc ctgagcttcg cacgggcact tagatcagcg
     3241 gatgcgtctt cacgacgtcg ctaaagatcg ccgccttcgc aaggcggagg ccgggggttc
     3301 aaatccccca gtgtcca
//
LOCUS       DAOSYN010000143         3285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_23024_length_3285_cov_2.522601, whole genome shotgun sequence.
ACCESSION   DAOSYN010000143 DAOSYN010000000
VERSION     DAOSYN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23024_length_3285_cov_2.522601"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /gene="aksA"
                     /locus_tag="PLJ11_05955"
     CDS             <1..462
                     /gene="aksA"
                     /locus_tag="PLJ11_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176519.1"
                     /note="in Methanococcus jannaschii this protein catalyzes
                     the condensation of alpha-ketoglutarate and acetyl-CoA to
                     form trans-homoaconitate; functions in alphaketosuberate
                     synthesis which is a precursor in coenzyme B and biotin
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitate hydratase"
                     /protein_id="HOO04249.1"
                     /translation="GNTPLEEIVMAAKHLLHMDVDYRTERFREVAEYVAKAAGREIPG
                     WKPIIGTNCFAHEAGIHTDGVIKYLSNYEPYTPEEVGLTRKIVIGKHSGRHTIKQILA
                     NKGYEIDDELAQRILDQVRANSIALKRSLSENELIYIYQDFIAGDNQVMRD"
     gene            1040..2233
                     /locus_tag="PLJ11_05960"
     CDS             1040..2233
                     /locus_tag="PLJ11_05960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO04250.1"
                     /translation="MRILLYTHEFPPFAGGAGVYTAGLAKGLSNLGHEVTVVAPAYGE
                     AIAQWDAQQPYTVDRMLLPGGRLTVLAGSIGLIRAWLHLRPDLTFVTDVGAQLAASMA
                     SLVFPFQAPYCITVHGSEIEWHFGIGRRKRPQALLMPVVRRFFARSLAIVCVSRATQE
                     ALLYKLPALGERVAVVHNGIDLDAFPPVTEEEVGRLRNKIDAPGPVLLTVARLIPEKG
                     IDNVLKALSRIAPDMPDLRYVVVGAGPDLGRLKAQVTDSSLEGNVVFAGKVSQRELSA
                     YYALCDIFIMPSRPSDRFEGFGLVYAEAGAYGKPVIAGRTGGVPEVVQDGHNGILVDP
                     MDVDGLERAIRSLLSNPGLARSMGCNGRRQVVERFNILRMAESTLQVVCERGGRPKHG
                     GGPGA"
     gene            complement(2964..>3285)
                     /locus_tag="PLJ11_05965"
     CDS             complement(2964..>3285)
                     /locus_tag="PLJ11_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04251.1"
                     /translation="ARNALLSAEDAERLPAADDIRKMSAACVRVLDEGVEALFRRDIE
                     QANAVIDEGMDVVRRCERFGKEVQVRDSREAIITHMVLDSIIRSTMYAVDIAEIAING
                     SMRS"
BASE COUNT          621 a         1034 c         1043 g          587 t
ORIGIN      
        1 gggaacaccc cgctagagga gatcgtcatg gccgccaagc accttctgca catggacgtg
       61 gactaccgca cggagcgctt ccgcgaggtc gccgagtacg tggccaaggc ggccggccgg
      121 gagatccccg gctggaagcc catcatcgga acgaactgct tcgcgcatga ggcgggcatc
      181 cacacggacg gggtgatcaa gtacctctcg aactacgagc cgtacacccc ggaggaggtc
      241 gggctcaccc gcaagatcgt catcggcaag cactccggaa ggcacaccat caagcagatc
      301 ctggccaaca aagggtacga gatcgacgac gagctggcgc agaggatcct ggaccaggtt
      361 cgcgcgaact cgatcgcgct gaagcggtcg ctctcggaga acgagctgat ctacatctac
      421 caggacttca tcgccgggga caaccaggtc atgagggact gagcgcgcca tcgtcagccc
      481 ggcccatgtg ggcgcaggcg cggtcgaggg cggccggtcc tccggcccct tcacgcccct
      541 cgatcgatcc ccaggcccag tgatcgctgg atggaccggt ggttcaagcc tgttgaaacc
      601 gcccaagcgt cctctccacg gcggttcaag gggccatgct ggcggccagg gcccggatgg
      661 gcacgtcccc atgcatgaac acccaagctc atgggggcgc gaactccgag gcgtctcggc
      721 atagagggca aatatagagg gcaaaggcac ctgcaggaaa gcctccctgg aggtctaccg
      781 gcgccctccg cggccgcctt gaatcccacg gtagtaaata atgggtccac atttcatagg
      841 gtatagctca acagtacgtc ggaacagggg ggcatgcatt gtcaaggacc ggcgcggatc
      901 gctcaggagc tcctgggggc aggggcttcg gggctcctat cctgagggag ggggccgacc
      961 tccggtcggg gcgatctcca atcagaatac catccgggag gatcttccgt tcaacaaagg
     1021 cgctgagtaa ggggcaacaa tgagaatact cctgtatacc cacgaatttc cgcctttcgc
     1081 ggggggagcg ggtgtctaca cagcaggcct cgccaaaggg ctatccaacc ttggccacga
     1141 ggtaacggtg gtggcgcctg cctacggaga ggccattgcc caatgggatg cccagcaacc
     1201 gtacaccgtc gatcggatgt tgctaccagg gggaaggctg accgtgctgg ctggaagcat
     1261 tgggctcatt cgggcctggc tgcacctacg gccggacttg acgttcgtga ccgacgttgg
     1321 cgcgcaactg gccgcttcga tggcctcgct ggtctttccg ttccaggcgc catactgcat
     1381 caccgttcac ggctcagaga tagagtggca tttcggcatc gggcgcagga aaaggcccca
     1441 ggcgcttctc atgcccgtcg tccggcgttt cttcgcccga tccctggcga tcgtttgcgt
     1501 gagccgagcc acccaagaag cgctcctata caagttgccc gcccttggtg agcgcgttgc
     1561 agtggtccat aacgggatcg acctggacgc tttcccaccg gtgaccgagg aggaggtcgg
     1621 cagactaagg aacaaaatag atgcaccggg accagtcctt cttacggtag cacgcctgat
     1681 ccccgagaag gggatcgaca atgtgctgaa ggccctttct cggatcgctc ctgacatgcc
     1741 cgacctgagg tatgtggtcg ttggggccgg tcctgacctg ggtcgcctta aggcccaggt
     1801 cacagattcc tccttggagg gcaacgtggt ctttgcagga aaggtgtccc agagggagct
     1861 gtcggcctat tatgccctat gtgacatctt catcatgcca agtcgcccct cggatcgctt
     1921 cgaggggttt ggcctggtgt atgcggaggc cggagcgtat ggaaagcctg tgatcgcagg
     1981 aaggacgggc ggcgtccccg aggttgtcca ggatgggcac aacggtatcc ttgtcgatcc
     2041 catggatgtg gatggactgg agagggcgat acgatcgctg ctttcgaacc cagggttggc
     2101 gcgaagcatg ggctgcaatg gtcgcaggca ggtggtggag aggttcaaca tcctgaggat
     2161 ggcggagagc acgcttcagg tcgtatgtga aaggggaggg aggcccaagc atggaggggg
     2221 accaggggct tgatcccgat agcatcacgc ccaccgctct attggtccat gatgacgctc
     2281 cctgagccgc ctatgcgggc gcatcgcacc gtggtcctca gcccctaagg atggggcacc
     2341 ggatcgccgc ccatcatttc atttgggggt cagcgtaaag ggcgatggca gcctcattgg
     2401 tccacatccc aagggcttgt tgcgaggtcg caatgaccgg gcaggccgat cgccgtggct
     2461 tgcagcaggg ccaataacga tagttacata gtctattgcc cttcctgggc atggcgctag
     2521 ggatcggcct caggcatcgg tccccaaccc ggcatcccac tcggcggcct gagcatgggg
     2581 gccgcccccc cgatggccag cggtccgccc gctcctcccg agggcatctg gaggggggaa
     2641 gggtgggacc gctcatctta tggaacctct cagaacgcag gggcacagcc cacagcgggc
     2701 caagcccaag ggaggatggt cagggctgga tttcaggggg ccttgagcgc ctcgacacgt
     2761 tcctctatct tggaccaagg gggggaatgc cgctccgggc cctccgatcc cagacccgcg
     2821 cgttcgcctc cgctgcggcc ttcagccgga gccccctccg atccctcacg cccgctcctc
     2881 ccctccgccc cgcccaccta tggaaggatg cctaggagca atcgcataat tgaccacgct
     2941 ctattgggcg cggcccgaga gcgtcagctg cgcatgctcc cattgatcgc gatctccgcg
     3001 atatcaacgg cgtacatcgt ggagcggatg atggagtcca ggaccatgtg ggtgatgatc
     3061 gcctcccggc tgtccctgac ctggacctcc ttcccgaacc gctcgcacct ccgcacgacg
     3121 tccatcccct cgtcgatgac cgcgttcgcc tgctcgatat cccttctgaa cagggcctcc
     3181 accccctcgt cgaggacgcg gacgcaggcc gcactcatct tcctgatgtc atcggccgcc
     3241 ggcagcctct ccgcatcctc cgcgctcagc agcgcgttcc gggcg
//
LOCUS       DAOSYN010000144         3275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_23111_length_3275_cov_3.635404, whole genome shotgun sequence.
ACCESSION   DAOSYN010000144 DAOSYN010000000
VERSION     DAOSYN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23111_length_3275_cov_3.635404"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLJ11_05970"
     CDS             complement(<1..667)
                     /locus_tag="PLJ11_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOO04252.1"
                     /translation="MALGVALDLGTSGYRGHLVDLDKKGKILATAITMRHPLPGANIM
                     DHLHFWMENGADVGHRIIIETVDKLIGTMGAKPEEITRVSVCGNPAQMSMFEKIEIRD
                     LAFAGQSILKRLNVKVPERKAHTIKAEELGMRSVKGTAEVRIPPSIRHEIGADALAMI
                     MKTGLMEKKETCMVTDYGTNAEMGLFHKGELYTGSAAAGPAMEGQSIDFGMLAAPQAI
                     SDLA"
     gene            873..1538
                     /locus_tag="PLJ11_05975"
     CDS             873..1538
                     /locus_tag="PLJ11_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO04253.1"
                     /translation="MRIAQVTGFLGSGKTTFLIEVAKELNARGLKVATIVNDVGHINV
                     DRKCMEVHGINTMEISGGCICCEVQGTLTTTVTNIFLSYRPDIILVEPTGVAIPLGLK
                     QAAAETVKKMPNVLEQAPIVTFVDAMRIDKLMNNVRRLVETQIVEADAVLINKVDAVS
                     DERLRQVTEMVRGLNPEVGILYGSSRTGQGVREVADIMMSGEAATYDLTEAEDRFDRK
                     YGE"
     gene            1543..1995
                     /locus_tag="PLJ11_05980"
     CDS             1543..1995
                     /locus_tag="PLJ11_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04254.1"
                     /translation="MDQKERQLLTHRASDELGKFSMWLRLYIKDPLDGKRAEEMVVDL
                     LDGITRECFARGADMIGHVKSFLTAEDGSTISVSLIDMDIPPTVQNRFDGTEMVRGEL
                     IVHVIVHGMWDPQVREASLDFTKGFMAERSIEYEVVNDFYEKEKRIKD"
     gene            2036..3085
                     /locus_tag="PLJ11_05985"
     CDS             2036..3085
                     /locus_tag="PLJ11_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO04255.1"
                     /translation="MTPRDRFDAAFDRAPADRPPIMYQHLGAARTVLRAAGLTMREGY
                     HDPEKFARICTMAQRITGFDNVMAGWGDILVEAHAHGTPWRWPEKDYYPRSDGYAIGS
                     LAQLDQVRSVDPMDDPYWSVPLRAAGLMQERIGEEVAVLGCITGPMMMGGEVMGYEAL
                     LMATWAEPDLAEELMGRLLGSSAAYGEHLARMGVGYVFIEDGTCSVDVNSIEGLQRFD
                     LGMLSRMLSAFRSQGLRSIVHNCANNPYLDGYPGMEIDALHFTPKAEERKGLYDRFRK
                     EMTVIGGIDQTVLLFKGSPEDVAREVQGMLADWGDGPGYMVAPGCEMPFKIPMENIIA
                     LREAVAGVRPLPRDG"
BASE COUNT          648 a          903 c         1075 g          649 t
ORIGIN      
        1 aggccaggtc ggaaatggcc tgcggagcgg ccagcatgcc gaagtctatg gactggcctt
       61 ccatggccgg gcccgcggcg gcggagcctg tgtagagctc gcccttgtgg aacaggccca
      121 tctcggcgtt ggtcccatag tccgtgacca tgcacgtctc cttcttttcc atgagcccgg
      181 tcttcatgat catcgcgagc gcatcggcgc ctatctcatg ccggatggac ggggggatgc
      241 gcacctcggc ggtgcccttg acggacctca tccccagctc ctctgccttg atggtatggg
      301 ccttcctctc cggcaccttg acgttgagcc tcttgaggat ggactgcccc gcgaaggcca
      361 ggtctcgaat ctcgatcttc tcgaacatgg acatctgggc ggggttgccg caaacggaca
      421 cccgggtgat ctcctcgggc ttggcgccca tggtcccgat gagcttgtcc acggtctcga
      481 tgatgatccg gtgcccgacg tctgccccgt tctccatcca gaagtgcaga tggtccatga
      541 tgttggcccc gggcaacgga tgcctcatgg tgatggccgt tgccaagatc ttccctttct
      601 tgtccaggtc gacaaggtgc ccccggtacc cgctagtgcc cagatccagc gcgactccca
      661 atgccatgga taacctcgga gggggaatgc cgtgaacgga tttataatac tgtttactat
      721 gctgtccccc catgccgatg ctatgctgcg catcgcactt tttatggtaa gatcgcgcat
      781 gttagccgcg tccagtctcg atgcggatag ggttgccaac gcatcggggg caatggaatt
      841 atatccgtga ggcttctacc ctccctgatt ccatgaggat agctcaggtg accggtttct
      901 tggggagcgg aaagaccacc ttcctcattg aggtggcgaa ggagcttaac gcgcgggggt
      961 tgaaggtggc caccatcgtc aatgacgtgg ggcatatcaa cgtcgaccgg aagtgcatgg
     1021 aggtgcatgg gatcaatacg atggagatct ccggcggctg catttgctgc gaggtgcagg
     1081 gcacgctgac caccacggtc accaacatct tcctgagcta taggccggac atcatccttg
     1141 tcgagccgac gggagtggcc atcccgctgg ggctcaagca agctgcggcg gagacggtga
     1201 agaagatgcc caatgtcctg gagcaggcgc ccatcgtgac ctttgtggac gccatgcgca
     1261 tcgacaaact gatgaacaac gtccgacggc tcgtcgaaac gcagatagta gaggcggacg
     1321 ccgtgctcat caacaaggtc gacgccgtaa gcgatgagcg ccttcgacag gtaacggaga
     1381 tggtccgggg gcttaacccg gaggttggca ttctctatgg ttcctcccgc accggacagg
     1441 gagtgaggga ggtggccgac atcatgatga gcggggaggc ggcaacgtat gaccttactg
     1501 aggcggagga ccgcttcgac cggaagtacg gggagtgagc cggtggacca aaaggaaagg
     1561 cagctgctga cgcatagggc ctccgacgaa ctgggcaagt tctccatgtg gcttcggctc
     1621 tatatcaagg accccttgga cgggaagcga gcggaggaga tggtcgtgga cctgctggac
     1681 gggatcaccc gggagtgctt cgctcgcggg gcggacatga tcgggcacgt caaatcgttc
     1741 ctcaccgccg aggacggctc cacgatcagc gtcagcctga tcgacatgga catccctccg
     1801 acggtccaga accggttcga cgggacggag atggtccggg gggagctaat cgtgcacgtc
     1861 atcgttcatg ggatgtggga cccccaggtg agggaagcct ccttggactt caccaaaggg
     1921 ttcatggccg agcgctccat cgaatacgag gtcgtcaatg acttctatga gaaggagaag
     1981 cggatcaagg actgagcgcg caaaggattt ctaggccgca cgcagatgag ggggcatgac
     2041 ccccagggat aggttcgatg cggctttcga tagggcgccg gcggacagac caccgatcat
     2101 gtatcagcac ctgggggcgg ccaggaccgt cctgagggcg gcggggctca ccatgcgcga
     2161 agggtaccat gatcccgaga agttcgcccg catctgcact atggcccaac ggatcactgg
     2221 gttcgacaat gtcatggccg gatgggggga catcctggtc gaggcgcacg cccatggcac
     2281 gccctggaga tggccggaga aggactacta ccctcgctcg gacgggtacg ccatagggtc
     2341 gttggcccag ctggaccagg ttcgatcggt ggatccaatg gacgatccgt attggtccgt
     2401 gccccttcgg gccgctggtc tcatgcagga acggatcggg gaggaagtgg cggtgctggg
     2461 gtgcatcacc gggccgatga tgatgggcgg ggaggtcatg ggctatgagg ccctgctcat
     2521 ggctacctgg gcggagccgg acctggccga ggagctgatg ggccgcctgc tcgggtcctc
     2581 ggccgcctat ggggagcact tggccaggat gggggtcggg tacgtcttca ttgaggatgg
     2641 gacatgcagc gtggacgtca attccatcga ggggttgcag cggttcgacc ttggaatgct
     2701 gtccaggatg ctctcggcct tccgttccca gggcctcagg tccatagtgc ataactgtgc
     2761 caacaatccc tatcttgatg gctatccggg catggagatc gacgccctcc atttcacccc
     2821 caaggccgag gagcgaaagg gcctctatga caggttccgc aaggaaatga cggtcatcgg
     2881 cggaatagac cagacggtcc tgctgttcaa aggctccccc gaggacgttg ccagggaggt
     2941 ccagggaatg ttggccgatt ggggcgatgg cccggggtac atggtggcgc cagggtgcga
     3001 gatgcccttc aagatcccga tggagaacat catcgctctg agggaggccg tggccggggt
     3061 ccgccccctg ccccgggatg gttgaacgcg tgttaattaa aattcgcggt cgtttcattg
     3121 ggcgaaggag atgccagcgt ctgtggaatt aacttgtgtt aatcgatgtc ctggatgctt
     3181 gcacccccga tagattaatg tacggtaaaa atgatactcg ggacaggtca gattcctact
     3241 ggactatagg gggaatgaat ttgcctgaaa aattg
//
LOCUS       DAOSYN010000145         3269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_23182_length_3269_cov_3.981332, whole genome shotgun sequence.
ACCESSION   DAOSYN010000145 DAOSYN010000000
VERSION     DAOSYN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23182_length_3269_cov_3.981332"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..679
                     /locus_tag="PLJ11_05990"
     CDS             248..679
                     /locus_tag="PLJ11_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04256.1"
                     /translation="MTAKVNFDAMDVVEQALFIIDNYADIFLDNLGLGLNRRRILSIM
                     YEARTRLMQEWRFPELVKVLTFLQETFHQDLQVEIDAFRSLMPLSREEVGDVIALRAD
                     QYFEMGNYRAALVHYQLLEAVAKGGLPSERAQECLARIDGA"
     gene            complement(682..1572)
                     /gene="rfbD"
                     /locus_tag="PLJ11_05995"
     CDS             complement(682..1572)
                     /gene="rfbD"
                     /locus_tag="PLJ11_05995"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOO04257.1"
                     /translation="MHRVMVVGAAGLLGQYLMRAGEGMGLAMVGTYNETLPGESAGTL
                     KMDISDPDSVARGFLAACPELVVLAAALTNVDQCEREPDRAYSVNMEGAFNVASECKD
                     AAVKMVYVSTDYVFNGLKGTRYHEFDVPDPQSVYAKSKLEGERVTLDADPGNLVCRVS
                     VLYGWNTLNGKSNFITWVIDSLGRGVPVPLYTDQYVSPTYAPAAARDILELGLRGARG
                     IYHTSGPDCLSRHEIGLRVAEAFGLDPSLVRPVTTEGMPQLAMRPPCSCLAVDMAEAE
                     LGHPMTSLKEGLALMRAARP"
     gene            1789..2343
                     /locus_tag="PLJ11_06000"
     CDS             1789..2343
                     /locus_tag="PLJ11_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO04258.1"
                     /translation="MRIAFVNGSPRPDGNTGSMLKLLAREAGARGAEVAYFEIVGKDI
                     EDCDGCYRCDVEDRCSKGDDMTAAYALIQASDVLVIGSPIYMGAETGLTKCFVDRLYY
                     LMAKQRLPAGKRAAALFTCGLVDGHVIYGYMHTRYDKLLRHDLGFEEAKTFIVPGTKH
                     RVDLEGNYYAQETLKEMAGFLFQE"
     gene            2357..>3269
                     /locus_tag="PLJ11_06005"
     CDS             2357..>3269
                     /locus_tag="PLJ11_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOO04259.1"
                     /translation="MKGCQHCGSQRLVPKVLVGGPMAGLDNKDGRYVCLDCGRELVPL
                     GFEDEAERKDFTLGKEDGGKGMLHIPIVPVDTWPLFSVPVLDVPLVQVAQVVELEWHE
                     DWHILPGGVPFADYLRATGSSRYGAEEVLLMDLAGLQRARPNFEALKALMKRKYSVWL
                     EMGVREVQDLFDAFTLGAETVLVGSMTCSSERLLKEVIELSDMSVPLLYHAGKVLWGA
                     AGLPAELPGALRQLSDLGYERAAVLDLRRLGGREGYDRELADLALSADIEIVFGGGVH
                     EPDLPELRAKGAVGALLDPYTPVLRDII"
BASE COUNT          570 a          979 c         1130 g          590 t
ORIGIN      
        1 tgcaggcgag cgcgtggtta aaccgtaagg aggttcggga tcattacggc atcagagcgt
       61 tcaattcgaa gcaacaaatg aaaatatcag gcaagattta ttaatcatgg cccgtttttt
      121 ttaattcaat ctttttatat cgatccaatt ttcccttttt gatgatgggg gcgtaggggg
      181 aaacgttcaa aggcgggaag ggcgatggag ccatctggcg tccgctaggg cgcctgcgag
      241 ggtgccgatg accgccaagg tgaacttcga tgcgatggac gtggtggagc aggcgctctt
      301 catcatcgac aactacgcgg acatcttcct ggacaacctc gggctgggct tgaaccgcag
      361 gcgcatcctg agcatcatgt atgaggcgcg cacgcgcctc atgcaggaat ggcgcttccc
      421 cgagcttgtc aaggtcctca ccttccttca ggagacattc caccaggacc tgcaggtgga
      481 gatagacgcc tttaggtccc tgatgccgtt gagcagggag gaggtcgggg acgtcatcgc
      541 cctgagggcg gaccagtact tcgagatggg gaactaccgc gcggcgctgg tgcactacca
      601 gctgttggag gccgtggcca agggaggcct cccgtccgaa agggcgcagg agtgcctggc
      661 caggatcgac ggcgcgtaac gctacggcct cgcggccctc atgagcgcca ggccttcctt
      721 gagggaggtc atcggatgcc cgagttccgc ctcggccatg tccaccgcca ggcacgagca
      781 gggcgggcgc atggccagct ggggcatccc ctcggtggtt accggccgga ccagggaggg
      841 gtcgagcccg aaggcctcag ccaccctcag cccgatctca tgccggctga ggcagtccgg
      901 accgctggtg tggtagatcc ccctcgcccc cctcagcccc agctccagga tgtccctcgc
      961 cgccgcgggg gcgtaggtcg gggaaacgta ctggtccgtg tacaggggta cagggacgcc
     1021 tcgtccaagc gagtcgatca cccaggtgat gaagttgctc tttccgttga gcgtgttcca
     1081 cccgtacagc accgacaccc ggcatacaag gttgccaggg tcggcgtcca aggtcacccg
     1141 ttccccctcc agcttgctct tcgcgtacac gctctggggg tccgggacat cgaactcgtg
     1201 gtacctcgtc cccttgaggc cgttgaagac gtagtccgtg gatacgtaga ccattttgac
     1261 cgcggcgtcc ttgcattccg aggccacgtt gaacgcccct tccatgttca ccgagtacgc
     1321 ccggtcgggc tcccgctcgc actggtcaac gttggtaagg gctgcggcga gcacgacgag
     1381 ctccgggcag gcggcgagga agcccctggc cacggaatcg gggtcggaga tgtccatttt
     1441 gagcgtgcct gcgctctccc ctggcagagt ctcgttgtag gtgccgacca tcgccagccc
     1501 catcccctcc ccggcgcgca tcaggtactg cccgagcagg ccggccgccc cgaccaccat
     1561 caccctgtgc atggggcccc tatccctttg gagtgattta tattttgaca ggaagatcgg
     1621 aacggtgaat gatccacggt atgatcattc cctttcccga gtgatttatg ttttgatagg
     1681 gtggagggac tggggacgga cggtcgagcg cgaggccggc actgccttcg cccccggggc
     1741 tggggcaagg attatgtctg gggtccccca tgcacgtggg aggctagcat gaggatagcg
     1801 ttcgtgaacg ggagcccccg cccggacggc aacaccggga gcatgctcaa gctcctagcg
     1861 agggaggcgg gggcgcgcgg ggcggaggtc gcctacttcg agatcgttgg taaggacatc
     1921 gaggactgcg acggctgcta tcggtgtgac gtcgaggacc ggtgcagcaa gggcgacgac
     1981 atgaccgccg cctatgccct catccaagca tcggacgtcc tggtcatcgg ctccccgatc
     2041 tacatgggcg cggagaccgg gttgaccaag tgcttcgtgg accgccttta ctacctgatg
     2101 gcgaagcaac gccttccggc cgggaagagg gccgccgcgc tcttcacctg cggcctggtc
     2161 gacgggcacg tcatctacgg gtacatgcac acccgctacg acaagctgct gcggcacgac
     2221 ctggggttcg aggaggcgaa gaccttcatc gtcccgggga cgaagcacag ggtggacctg
     2281 gaggggaact actacgcgca ggagacgctc aaggagatgg cagggttcct cttccaggag
     2341 tgaggcccct gggaccgtga agggctgtca gcactgcggc tcccagcggc tggtgcccaa
     2401 ggtgctcgtg ggcggcccta tggccgggct cgacaacaag gacgggaggt acgtctgcct
     2461 ggactgcggc cgggagctgg tccccctggg cttcgaggac gaggcggagc ggaaggactt
     2521 caccctgggt aaggaggatg gggggaaggg catgctgcac attcccatcg tccccgtgga
     2581 cacctggccc ctgttcagcg ttccggtcct ggacgtcccg ctggtgcagg tcgcgcaggt
     2641 cgtggagctg gaatggcatg aggattggca catcctgccg ggcggggtgc ccttcgccga
     2701 ctatctgagg gcgaccgggt cctcgcggta cggggcggag gaggtcctgc tgatggacct
     2761 agcgggcctg cagcgcgccc ggccgaactt cgaggccctc aaggccctca tgaagcgcaa
     2821 gtactcggtc tggcttgaga tgggggtgcg ggaggtgcag gacctcttcg acgccttcac
     2881 cctgggggcg gagacggtgc tggtggggag catgacctgc tcttccgagc ggctattgaa
     2941 ggaggtcatc gagctctcgg atatgagcgt gccccttctg taccatgccg ggaaggtgct
     3001 ctggggagcg gcagggctgc ccgcggagct gccgggggcg ttgcggcagc tctcggacct
     3061 gggatacgaa cgggcggcgg tgctggacct ccgccggctc gggggaaggg agggctacga
     3121 ccgggagctg gccgacctgg cgttgagcgc ggacatcgag atcgtgttcg ggggcggggt
     3181 gcacgaaccg gacctgccgg agctgagggc gaagggggcc gtcggggcgc tcctcgaccc
     3241 ctacaccccc gtactgaggg acatcatcc
//
LOCUS       DAOSYN010000146         3247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_23390_length_3247_cov_4.120301, whole genome shotgun sequence.
ACCESSION   DAOSYN010000146 DAOSYN010000000
VERSION     DAOSYN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23390_length_3247_cov_4.120301"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLJ11_06010"
     CDS             complement(<1..275)
                     /locus_tag="PLJ11_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04260.1"
                     /translation="MQRIELNGSKVYVLPVIKGLVSEGEKVRAAILETRPEAVGVSIS
                     REELEGLRSYQGEEIGLSALEEAYRAGLEEFGEVQLPPPCYLEALRA"
     gene            complement(262..1221)
                     /locus_tag="PLJ11_06015"
     CDS             complement(262..1221)
                     /locus_tag="PLJ11_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOO04261.1"
                     /translation="MQREELIRSVRELLLRSGFRASMPLRLRSIAFDIVARRDRSLLL
                     VKVLTNIDAFSKENAEELKVLAEALEGSIVLVGERSGSGDLEPGIVYSRFDIPIIAYE
                     TMKALLLEDEPPFIFAAPGGLYVRLDSDLLHRIREDRGLSLGTLAEVAGVSRRTIQMY
                     EAGMGAMIDAALRLEEYLERPLVVPVDPMGYRPPPREPRPLEMERYDEFSRNIYTMLT
                     QLGLVVRPTEKCPFEALTGDRDLLLLTGLGKDEARLADKAKVVADVARITGKESVIFI
                     ERLRTRQSIEGTALIGRDELQKIKESERLRKMVNARSEVDAKD"
     gene            complement(1285..1794)
                     /locus_tag="PLJ11_06020"
     CDS             complement(1285..1794)
                     /locus_tag="PLJ11_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOO04262.1"
                     /translation="MRRMRRDDDWSGLFRSFDEEFDAMRERMDRLMEAALRGTGENPL
                     VYGFSMRTGPDGRPMVQEFGNVPRGGGLPPDTSYREPLTDVVEEEGRVKVIVELPGVE
                     KKDIDLRAEDRQLTISVDTERKRFNKTLELPCEVRPESASAEYRNGVLTVYLDRVPSV
                     PKGRKIDIK"
     gene            complement(1799..>3247)
                     /locus_tag="PLJ11_06025"
     CDS             complement(1799..>3247)
                     /locus_tag="PLJ11_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO04263.1"
                     /translation="SGPEIMSKFYGESEERLREIFKQAEENAPSIIFIDEIDSIAPKR
                     DEVSGEMERRIVAQLLALMDGLEARGKVVVIGATNRPNALDPALRRPGRFDREIEINI
                     PNREGRLDILQIHTRGMPLAEDVDLERLADLTHGYAGADLAALCKEAAMHSLRRILPD
                     LDLEADSIPAEVLNRITVQKDDFFAALREMSPSSMREVLVESPNLHWDDIGGLDDVKA
                     ELKEAVEWPLKYAKLFEHMNAEPPKGVLLYGPPGTGKTMLAKAVATESESNFISIKGP
                     EFLSKWVGESEKAVRETFRKARQAAPCVIFMDEIDSIAPTRGGDSDSHVTERVISQLL
                     TELDGLEALHNVVVIAATNRPDIIDPALLRPGRFDRLIQVGAPDLETRKAILEVHFRR
                     KPLADDVDLKDIALRTDGYTGADLAAIANEAVMAAIREAVASGKGEDKEALKDLKVQM
                     RHILGAVEKHRPLSLQELSKFQKIAKDFEYVR"
BASE COUNT          487 a         1181 c          965 g          614 t
ORIGIN      
        1 cacgcccgga gcgcctctag gtagcacggc ggcggcagct gcacctcccc gaactcctcc
       61 agccctgccc ggtacgcctc ctcaagggcg ctcaggccga tctcctcccc ctggtaggaa
      121 cgcagcccct ccagctcctc cctcgatatc gagacgccca ccgcctccgg tcgggtctcg
      181 aggatggccg cgcggacctt ctccccctcg ctgaccaatc ccttgatcac cggaaggacg
      241 tacaccttcg aaccgttcag ctcaatcctt tgcatccact tcgctccggg cgttgaccat
      301 cttgcgcagg cgctccgact cctttatctt ctgcagctcg tcacgtccga tgagcgcggt
      361 gccctcgatg ctctgtctgg tgcgtagcct ctcgatgaat ataaccgatt ccttgccggt
      421 gatgcgcgcg acgtccgcca ccaccttagc cttgtccgcc aacctggcct cgtccttccc
      481 caggccggtg aggagaagga ggtcccggtc gcccgtcagc gcctcgaagg ggcacttctc
      541 cgtcggcctc accaccagcc ccagctgggt cagcatggtg tagatgttgc ggctgaactc
      601 gtcgtacctc tccatctcca gcgggcgcgg ctcccggggc ggggggcgat accccatggg
      661 gtccaccggg accaccagcg gcctctcgag gtactcctcc agccgcaggg cggcgtcgat
      721 catcgccccc atgccggcct cgtacatctg gatggtgcgg cgggacaccc ccgccacctc
      781 cgccagggtg ccgaggctga gcccccggtc ctcccggatg cggtgcagga ggtcgctgtc
      841 aaggcggacg tacagcccgc cgggcgcggc gaagatgaac ggaggctcgt cctccaggag
      901 cagggccttc atcgtctcgt acgcgatgat cggaatgtcg aagcgcgaat agacgatgcc
      961 cggctccagg tcgccggagc cggagcgctc cccgacgagc acgatcgacc cctccagcgc
     1021 ctccgccagg accttgagct cctcagcgtt ctccttcgag aacgcgtcga tgttggtgag
     1081 caccttcacg agcagcaggc tgcggtccct tcgtgcgact atatcgaagg cgatgctcct
     1141 cagcctaagg ggcatcgagg cgcggaagcc ggagcgaagc agcagctccc gcacggaccg
     1201 gatgagctct tcgcgctgca cggatgtaat tcgctataac ttctatataa tgattattaa
     1261 aaagtcaagg gggccaggcc ccgttcattt gatgtcgatc ttccggccct tcggcacgga
     1321 gggcacccgg tccaggtaca cggtgagcac cccgttgcgg tactccgcgc tggcgctctc
     1381 cgggcggacc tcgcacggca gctccagggt cttgttgaac cgcttgcgct ccgtgtccac
     1441 ggagatggtg agctggcggt cctccgccct gaggtcgatg tccttcttct ccacgcccgg
     1501 gagctcgacg atcaccttca cccgcccctc ctcctccacc acgtccgtga gcggctcgcg
     1561 gtagctggtg tccggaggga gaccgccccc tcggggcaca ttaccgaact cctgcaccat
     1621 cggcctcccg tcgggcccgg tgcgcatgga gaacccgtac accagggggt tctccccggt
     1681 gccgcgcaag gctgcctcca tcagccggtc catgcgctcg cgcatcgcat cgaactcctc
     1741 gtcaaaggaa cggaacagcc cgctccaatc atcatcacgt cgcatcctcc tcatactatc
     1801 acctcacgta ctcgaaatcc ttggcaatct tctggaactt gctcagctcc tgaagggaga
     1861 gggggcggtg cttctccacc gcccccagta tgtgcctcat ctgcaccttc aggtccttca
     1921 gcgcctcctt gtcctcgccc ttgccggacg ccacggcctc gcggatcgcg gccatgaccg
     1981 cttcgttggc gatggccgcc aggtcagcgc cggtgtaccc gtcggtccgg agcgctatgt
     2041 ccttcaggtc gacgtcgtcc gccaggggct tgcgcctgaa gtggacctcc aggatggcct
     2101 tcctggtctc aaggtccggc gctcccacct ggataagccg gtcgaagcgc ccggggcgca
     2161 gcagcgccgg gtctattatg tccggccggt tcgtggcggc gatgaccacc acgttgtgca
     2221 gcgcctcgag gccgtccagc tcggtcagca gctgggagat caccctctcc gtcacgtggg
     2281 agtcggagtc cccgccacgg gtgggcgcta tcgagtcgat ctcgtccatg aagatgacgc
     2341 acggcgccgc ctgcctagcc ttgcggaacg tctcgcgcac cgccttctcg ctctcgccca
     2401 cccacttgct caggaactcg ggccccttga tgctgatgaa gttgctctcc gattccgtgg
     2461 ccaccgcctt cgccagcatg gtcttgcccg ttccaggggg cccgtacagc agcaccccct
     2521 tcggaggctc ggcgttcatg tgctcgaaca gcttggcgta cttgagcggc cactccaccg
     2581 cctccttcag ctccgccttt acgtcgtcca gcccgccgat gtcatcccaa tggaggttcg
     2641 gcgactcgac cagcacctcg cgcatgctgg acggagacat ctcccgcagg gcggcgaaga
     2701 agtcgtcctt ctgcacggtg atgcggttca gcacctcggc tgggatgctg tcggcctcca
     2761 ggtccaggtc gggcaggatc ctccggaggg agtgcatcgc ggcctccttg cacagcgccg
     2821 ccaggtccgc cccggcgtac ccgtgggtga ggtccgccag gcgctcgagg tccacgtcct
     2881 ccgccagggg catcccgcgg gtgtggatct gcaggatgtc caggcggccc tcccggttgg
     2941 ggatgttgat ctcgatctcc cggtcgaacc tgccggggcg gcgcagcgcg gggtccaggg
     3001 cgttcggccg gttggtggcg cctatgacca ccaccttccc gcgcgcctcc aggccgtcca
     3061 tcagcgccag gagctgggcc actatcctcc gctccatctc ccccgacacc tcgtccctct
     3121 tgggggcgat ggaatcgatc tcatcgatga agatgatgga cggggcgttc tcctccgcct
     3181 gcttgaagat ctcccgcagg cgctcctcgc tctccccgta gaacttgctc atgatctccg
     3241 ggccgct
//
LOCUS       DAOSYN010000147         3225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_23609_length_3225_cov_2.802208, whole genome shotgun sequence.
ACCESSION   DAOSYN010000147 DAOSYN010000000
VERSION     DAOSYN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23609_length_3225_cov_2.802208"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLJ11_06030"
     CDS             complement(<1..250)
                     /locus_tag="PLJ11_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HOO04264.1"
                     /translation="MELIVVDGIDGSGKSTVAEWLADHYRRRGETVLVRTHPSSSIFG
                     RLTRRALASEGRAMRALASVLFIADVLHSLHLLRSWKGY"
     gene            complement(260..976)
                     /locus_tag="PLJ11_06035"
     CDS             complement(260..976)
                     /locus_tag="PLJ11_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04265.1"
                     /translation="MLGGDALGLIMVYTYVGAIAALAARWRFLRERSLQRKFIHVMVG
                     NIALFWWVFDTRYVMALLAAAPFIPLLLLLSRKAGEGDGSTAGPKGSVLTTASHEGHQ
                     LGLVYYAISWTLLAFFAFEDLVVASVGIVCMAYGDGMGGLIGKAFGRRKVRGNKTVEG
                     TLAVFLFSVLGIFLIINYYNLLFSLGLYGTRTIALPLSALTAVALGAYVAIVELYTPG
                     EYDNLVIPITTALIMALAGI"
     gene            1123..1761
                     /locus_tag="PLJ11_06040"
     CDS             1123..1761
                     /locus_tag="PLJ11_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04266.1"
                     /translation="MNARDMRQYENFRRQLGPVPRPGLMVLDVRTTQFVVVELVLLKA
                     LIGDGNPGLFVSVDRPHQYMVHLLDMHGIEHTSVTFIDTVSQFAATDTCRQARVGFFK
                     GPNNIDMLPSALREWSSKDQGFDMAQCRFALIDNISNLLTFNGHEKVQRFIREFNDLL
                     GDRTAVVLVIDRQRNPNLYHSVLAMGGTELMLETELKDRLMDSEEGIDHGRQ"
     gene            1748..2449
                     /locus_tag="PLJ11_06045"
     CDS             1748..2449
                     /locus_tag="PLJ11_06045"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOO04267.1"
                     /translation="MEGNERDRTRLERIPSGVPGLDELIEGGFPFPSVILVSGAAGTG
                     KTTFCLRFLCEGAAKREQGLFFTTLSEPSHWMLRFSSQFDFMRPEYFGDAIVYEDLSV
                     LLRHGDQDKLLEAIDRRIAEVMPQRIVIDPITVVGEMLKDQYRSFLFDLTNRLKHWNA
                     TTLVTGEVPPGELYPPEIAHSVDGIVLLHLTEEYGDRRKYLEVLKMRGTNHSTGKHSI
                     DISRKEGLVVLKARF"
     gene            2605..>3225
                     /locus_tag="PLJ11_06050"
     CDS             2605..>3225
                     /locus_tag="PLJ11_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOO04268.1"
                     /translation="MLDDPLYLTIPIAGLVGLAFAGILTWWILRKDTGTPEMNAISDA
                     IREGAMAYLARQYKTIAIFSAILGVLIAIGINWLTGLAFLLGAFFSALSGFIGMYVSV
                     NSNIRTASAAKRTLNEALLTSFRGGAVSGIAVVALSLLGVASIFYVYQGWLVPDAGTD
                     EGFRQALFLGVGYAFGASFAALFARLGGGIYTKAADVGADLVGKVEA"
BASE COUNT          670 a          970 c          978 g          607 t
ORIGIN      
        1 catacccctt ccagctccga agaaggtgga gggagtggag gacatcggcg atgaaaagga
       61 ccgaggccaa tgcccgcatc gccctgccct cggaggcaag cgcgcgtcgg gtcaaccggc
      121 cgaagatgga ggaggaggga tgggtgcgca ccagcaccgt ctcccctcgc cggcggtaat
      181 ggtcggcgag ccattcggcc actgtgctct tccccgagcc atcgatgccg tcgaccacta
      241 tcaactccat gtctttccct cagatgcccg ccagggccat gatcaatgcg gtggtaatag
      301 ggatgacgag gttgtcgtac tccccggggg tgtacagctc cacgatcgcc acataggccc
      361 ccagggccac ggcggtcagt gcgctgaggg gcagggctat cgtcctggtg ccgtagagcc
      421 cgagggagaa cagaagattg tagtagttaa taataagaaa gatccccagg acggagaaga
      481 ggaatacggc cagcgtccct tctacggtct tgttcccgcg aaccttccgc ctgccaaagg
      541 ccttgccgat cagcccgccc atcccatccc cgtaggccat gcagacgatg ccgaccgagg
      601 ccaccaccag gtcctcgaag gcgaagaagg ccaggagcgt ccaggaaatg gcatagtaga
      661 ccaggcccaa ttgatggcct tcgtgcgagg cggtggtgag gaccgagccc ttcggtccgg
      721 cagtcgaccc gtccccttcc ccggcctttc tcgagagcag gaggagcagg gggatgaaag
      781 gggcggccgc cagcagggcc attacatacc tggtgtcgaa tacccaccag aacaaggcga
      841 tgttgccgac catcacgtgg atgaacttcc tctggaggga ccgttccctt aggaagcgcc
      901 acctggcggc gagcgccgct atggcaccga cataggtgta gaccatgatc agcccgaggg
      961 cgtcgccacc tagcaatacg atccctcccc catctcgctc tggatattta atagagaatg
     1021 gactccacgc gggctttacc agcccgcagg gtcgctatca attccaataa gcaccagaaa
     1081 gtagctaaat acccttgacc atgctaccag ggacggacgt ccatgaatgc cagggacatg
     1141 cgccagtacg agaacttccg taggcagctc gggcctgtcc cacgacccgg gctcatggtg
     1201 ttggacgtca ggaccaccca gttcgtggtc gtggagctgg tcctgctcaa ggccctcatc
     1261 ggcgatggga atccaggcct gttcgtctcg gtggaccggc cgcatcagta catggtccat
     1321 ctgctggaca tgcatggcat cgagcatacc agcgtgacgt tcatcgatac tgtatcacag
     1381 ttcgcggcca ctgatacgtg ccggcaggct agggtaggat tcttcaaggg accaaacaat
     1441 atcgacatgc tccccagcgc cctgcgggag tggtcctcaa aggaccaggg cttcgacatg
     1501 gcccaatgcc ggttcgcgct catcgacaac atctccaacc tcctgacgtt caatggccat
     1561 gagaaggtcc aaaggttcat aagggagttc aatgacctac tgggggacag gaccgcggtc
     1621 gtgcttgtaa tcgacaggca gaggaacccg aacctctacc attcggtcct ggcgatgggc
     1681 gggaccgagc tgatgcttga gaccgagctt aaggatcgtt taatggacag cgaagagggg
     1741 attgaccatg gaaggcaatg agagggaccg gacgaggctg gagaggatcc catccggagt
     1801 gccgggattg gacgagctaa tagagggagg gttccccttc ccatcggtga tattggtctc
     1861 cggggccgcc gggacgggga agaccacctt ctgtctccgt ttcctctgcg agggggcggc
     1921 caaacgcgaa caggggctgt tcttcacaac attgagcgag ccgtcccatt ggatgctgcg
     1981 cttctcctcc caattcgact tcatgcgacc cgagtacttc ggcgatgcca tcgtctatga
     2041 ggacctgagc gtccttctcc gccatggaga ccaggacaaa ctgctcgagg cgatcgatcg
     2101 gcgcatcgcg gaggtgatgc cgcagcgcat cgtcatcgac cccatcaccg tggtcgggga
     2161 gatgctcaag gaccagtacc gcagcttcct gttcgacctg acgaacaggc ttaagcactg
     2221 gaacgccacc acgcttgtga cgggggaggt cccccccggg gagctttacc ctccggagat
     2281 cgcccactcc gtggatggga tagtgttatt gcacctgacc gaggagtacg gggaccgtcg
     2341 caagtacctc gaggtgctga agatgcgcgg gaccaaccac agcaccggca agcactccat
     2401 cgatatctcg agaaaggaag ggctggtcgt gctcaaggcc aggttctgag cgcgttcccc
     2461 gcgcgccccc tgtccccttg ccatcggccc tttgggacaa tatgcggcag gtccgaagcc
     2521 agactacgat tttcgtggcc tgggaatacc taaacaattt atatggggag cagtatttag
     2581 gcaggaccta aaagaggttt tgtaatgtta gacgatcctc tatacctaac gataccgata
     2641 gcggggctgg tcggactggc attcgctggg attctcacat ggtggatcct cagaaaggac
     2701 actggcaccc ctgagatgaa cgccatcagt gacgctatcc gcgaaggcgc aatggcctac
     2761 ctggcccgac agtacaagac gatcgccatc ttcagcgcca tcttgggggt cctgatcgcc
     2821 atcgggatca actggctgac tgggctggcg ttcctgttgg gagcgttttt ctccgctctc
     2881 tccggattca tcggaatgta cgtgtctgtg aactcgaaca tcaggaccgc cagtgccgcc
     2941 aagcgcacac tgaacgaggc cctcctgaca tccttccgcg gcggcgccgt ctccggcatc
     3001 gccgtggtgg ccttgagcct gttgggggtc gccagcatct tctacgtgta ccagggctgg
     3061 ctcgttcccg atgccgggac cgatgaaggc ttccgccagg cgctgttcct gggcgttgga
     3121 tacgccttcg gcgcctcctt cgccgcgctc ttcgcccgac tgggcggagg gatctacacc
     3181 aaggccgccg acgtcggcgc cgaccttgtg ggcaaggtcg aggcc
//
LOCUS       DAOSYN010000148         3220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_23659_length_3220_cov_2.529542, whole genome shotgun sequence.
ACCESSION   DAOSYN010000148 DAOSYN010000000
VERSION     DAOSYN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23659_length_3220_cov_2.529542"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PLJ11_06055"
     CDS             <1..1070
                     /locus_tag="PLJ11_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04269.1"
                     /translation="ADNIFGREDSGGIPAIAELVANTQQPVVLIVNDFYELSRKSAAI
                     KNGTLQLKLSRVQAGTMRNVLRRIALDQGLEVPQRVFETIIENSNGDMRAAIRDLQAV
                     AEGNVEMGEDDAFVLESRMSVKSMYDLMRDVLHEGDSTRARRTQMNVDEPPEQVMMWL
                     DENVPLEYRDPEDLWRAYEHLSRADIFLARATRRQYFGLWAYATDHNIVGVCSAKSRP
                     SRGWVQYRFPSYIMKMSRSKAYRAMRKGIAYKISAYTHTSTRRSAQDVLPYLTELFRA
                     DREFRLAMISAMRLTIEEVAFLLDKKVDAAEVKQLMQEAERSRATGEKAAASPPQRAP
                     EEEKKERRPEPKGKQASLFEY"
     gene            1080..2012
                     /gene="twy1"
                     /locus_tag="PLJ11_06060"
     CDS             1080..2012
                     /gene="twy1"
                     /locus_tag="PLJ11_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HOO04270.1"
                     /translation="MKDDLREILQKQHYRLCGDHAAVKLCHWMKESLLRGRTCYKQDF
                     YGIRSHRCLQMTPVVNDCTHNCLFCWRVRGFESTVARWQEPEAVLDACIEGQRRLASG
                     FKGDPRCPPAMWKEAQEPKQVAISLAGEPTMYPYLGDLIGLCHRRGMTTFLVTNGTFP
                     GVLEALSPLPTQLYVTVAAPNKEVYRTLCVPRVPDGWERLMRTLELLPSLGTRTVVRH
                     TLVKGWNLGWEKEYAALDRIASPLFVEPKGFVFVGDSRRRMTMDNMPSHAEVVEFGRR
                     VGDELGLCLLNEKPESRVAVFGEQGMVTTIPGLA"
     gene            complement(2009..2800)
                     /locus_tag="PLJ11_06065"
     CDS             complement(2009..2800)
                     /locus_tag="PLJ11_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO04271.1"
                     /translation="MPKADIGDVRINYEVHGEGEPVVLITGLGGEIAFWRRYIPLLSR
                     AHQLVVLDNRGAGETDYPSTLFTVGNMAEDVAGLMRHLGHDRYHVLGWSMGGNVAQRL
                     AIDHPERVGALVLMSTYARRPERSAFAIDTMIRTVDEGASFDTLMELMQSWCLPEDYF
                     RKLALERPAQRRCNGNGKRAAEGFRAQKLALDLFDSHGHLGAIVSPTLIVHGAEDIMV
                     APHFALELHESIKGSELEWVPGAGHIIPADRCCHRVLGFLGRHPL"
     gene            complement(2857..>3220)
                     /locus_tag="PLJ11_06070"
     CDS             complement(2857..>3220)
                     /locus_tag="PLJ11_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04272.1"
                     /translation="LMSLEGLSAWDLLLLIGLFAEQTWLMRSFQSIMSRRMALQLLNV
                     RIDKLEELVERANGLLASGEDELERLSALDALLKEYLSMMVYDLYRLDLLGHLPVYIV
                     GPRKKYALDERVLSHFPG"
BASE COUNT          615 a         1019 c         1057 g          529 t
ORIGIN      
        1 aggcggacaa catcttcggc cgggaggact cgggcgggat accggccatc gccgagctgg
       61 tggcgaacac ccagcagccg gtcgtcctga tcgtcaacga cttctacgag ctgagccgca
      121 agagcgcagc catcaagaac ggcacgcttc agctcaagct ctccagggtg caggcgggga
      181 caatgcgcaa cgtccttcgc aggatagcgc tcgaccaggg gctggaggtt ccccagaggg
      241 tcttcgagac catcatcgag aactccaacg gggacatgcg cgccgctatt agggacctcc
      301 aggccgtggc cgaaggaaac gtggagatgg gcgaggatga cgccttcgtc ctggagagca
      361 ggatgtcggt caaaagcatg tacgacctca tgcgggacgt tctgcacgag ggcgattcca
      421 ccagggcccg ccgcacccag atgaacgtgg acgagccccc cgaacaggtc atgatgtggc
      481 tcgacgagaa cgtcccgctg gagtacaggg accctgagga cctctggcgg gcgtacgagc
      541 acctgtcgag ggcggatatc ttcctggcga gggcgacaag gcgccagtac ttcggcctct
      601 gggcctatgc gaccgaccat aatatcgttg gggtatgctc ggccaagtcc agaccgagca
      661 ggggctgggt gcagtaccgc tttccctcct acatcatgaa gatgtcccgc agtaaggcct
      721 accgtgccat gaggaagggc atagcgtaca agatctcggc gtatacgcac acctccaccc
      781 gccgctccgc ccaggatgtc ctgccctacc tgacggaact cttcagggcc gatcgcgagt
      841 tccgcctggc catgatcagt gccatgcgcc tgacaataga ggaggtggcc ttcctcctcg
      901 acaagaaggt ggacgctgcg gaggtcaagc agctgatgca ggaggcggaa cggtcgaggg
      961 cgaccgggga gaaggctgcc gcgtccccgc cgcagagggc accagaagag gaaaaaaagg
     1021 agaggaggcc ggagcccaag ggaaagcagg cctcgctctt cgagtactga ggatggggca
     1081 tgaaggacga cctgagggag atcctgcaga agcagcatta ccggctgtgc ggcgaccacg
     1141 ccgcggtgaa gctctgccat tggatgaagg agagcctgct caggggaagg acctgctaca
     1201 agcaggactt ctacggcatc cgttcacacc gctgccttca gatgacgccg gtggtgaacg
     1261 actgcacgca caactgcctg ttctgctggc gcgtgcgggg gttcgagtcc acagtcgcca
     1321 ggtggcagga gccggaggcg gtgctagatg cgtgcatcga ggggcagagg cggctggcct
     1381 caggcttcaa gggcgatccc cgctgccccc ctgccatgtg gaaggaggcc caggagccca
     1441 agcaggtggc catctccctt gccggggagc ccacgatgta cccgtacctg ggggatctca
     1501 tagggctgtg ccatcgccgc gggatgacga ccttcctggt caccaatggc acgttccccg
     1561 gggtgctcga agccctttct cccctcccta cccagctcta cgtgacggtc gccgccccca
     1621 acaaggaggt gtaccggacc ctatgcgtcc cccgcgtccc ggacgggtgg gagaggctca
     1681 tgcggaccct ggagctgctg ccctcgctgg gcacgcgcac ggtggtcagg cataccctgg
     1741 tcaagggctg gaacctcggc tgggagaagg agtacgccgc cctggaccgc atcgcctccc
     1801 ccctgttcgt ggagcccaag ggcttcgtct tcgtcgggga ctcccgccgc cgcatgacca
     1861 tggacaacat gccctcccac gccgaggtgg tcgagttcgg gaggagggtg ggagacgagc
     1921 tcgggctttg cctgctgaat gagaaaccgg aaagccgggt ggcggtgttc ggggaacagg
     1981 gaatggtcac aacgatcccc gggctagcct agagcggatg gcgccccagg aagcccagga
     2041 cgcgatggca gcacctgtcc gcggggatga tgtgcccggc gccagggacc cactccagct
     2101 ccgagccctt gatgctctcg tggagctcaa gggcgaagtg cggggcaacc atgatgtcct
     2161 ccgccccatg gactataagc gtgggcgaga cgatggcccc gaggtgcccg tggctgtcaa
     2221 aaaggtccag ggccagcttc tgggcgcgga acccctccgc cgcccgcttg ccgttcccgt
     2281 tgcaacgccg ctgggcgggc cgctccaacg ccagcttgcg gaagtagtcc tccggcaggc
     2341 accacgactg catgagctcc atgagcgtgt cgaaggacgc cccctcgtcc acggtacgga
     2401 tcatggtgtc tatggcgaag gcggagcgct ccggcctgcg ggcataggtg gacatgagca
     2461 ccagcgcccc caccctctcg gggtggtcga tcgccagccg ctgggcgacg ttgccgccca
     2521 tggaccatcc caggacatga tagcggtcat ggcccaggtg gcgcatcagc ccggccacgt
     2581 cctcggccat gttgcccacg gtgaagagcg tcgacggata gtccgtctcc ccggcccccc
     2641 ggttgtcgag caccaccagc tgatgcgccc tgctcaggag gggaatgtat cttctccaga
     2701 aggcgatctc ccctcccagc ccagtgatca ggacgaccgg ctcgccctcg ccatgcacct
     2761 cgtagttgat gcgcacgtcc ccgatgtcgg cctttggcat tcggacgcct ggatatggct
     2821 catgctattt gtcattattc tttacgggag gggcgctcag ccgggaaaat gggagagcac
     2881 cctctcgtcc agcgcgtact tcttgcgcgg cccgacgata tatacaggga ggtgcccgag
     2941 gagatcgagg cggtataggt catagaccat catcgaaagg tactccttca ggagggcgtc
     3001 gagggcggaa agcctctcca gttcatcctc accgctcgcc aggagcccgt tggccctctc
     3061 gacgagttcc tcgagcttgt cgatgcgcac gttcaggagc tgcagcgcca tccgcctgct
     3121 catgatgctc tggaagctcc tcatcagcca ggtctgctcg gcgaagagcc ctatcaggag
     3181 gagcaggtcc cacgcgctca ggccctcgag ggacatgaga
//
LOCUS       DAOSYN010000149         3216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_23687_length_3216_cov_3.613097, whole genome shotgun sequence.
ACCESSION   DAOSYN010000149 DAOSYN010000000
VERSION     DAOSYN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23687_length_3216_cov_3.613097"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLJ11_06075"
     CDS             complement(<1..803)
                     /locus_tag="PLJ11_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO04273.1"
                     /translation="MKATARTMGISYAIREMLLPARELEKQGHRIIKLHIGDPNKFDF
                     KTPKYVRDALCAAVEKCDNGYEESEGNLELRKAIIEKERKKNRIDLALKDMIVTNGVT
                     EALQMVCAATIEVGDEALVPGPSYTSYLEFPKFFGGKAITYRTMEEEGWQPDIDDLRK
                     KITPRTKFIAVINPNNPTGAVYTAKRLKEIADLAGEFGLFIISDEIYDLMTFEGEHHS
                     PATLAKDVPVILVNGFSKVDLLPGWRLGYAAFHDTTGQLEEIEEAVHKQA"
     gene            949..2868
                     /locus_tag="PLJ11_06080"
     CDS             949..2868
                     /locus_tag="PLJ11_06080"
                     /inference="COORDINATES: protein motif:HMM:NF010368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOO04274.1"
                     /translation="MAEVRLGKMHLRWCDSCHVPVLEDAQCGRCGTATRQVELTPPGD
                     ARPAFAYDLERARGLLDRQFGEGSGIALLPEGSVYLMNRAPALDRMDEVIADGEVVAT
                     LRYDLGVGWRALPRVRGAAMIVRTASRNLVWADQGAVAPILASQNLMAPGVAKVQGDF
                     QRGEEVIVLDPHGNALATGVARMSSQEMRGGGRGVAVKVRWAERLEAPPERGRADWGV
                     ALEANARVMERRVRESVGFIRRLVEEHGDLPKVVSFSGGKDSLATLLLTREAGLDLPV
                     LFIDTGLEFPETVEYVHRLKEELGLDLVVGKADEEAFFKNVEFFGPPGKDYRWCCKTN
                     KLGPTVKAIVDHYPEGVLSFIGQRRYESEQRAAKPRVWRNPWTPGQIGASPIQDWTAL
                     HVWLHIMRSGVSYNPWYERGLDRIGCFLCPASDMAELEIARSSPSYERWEAFLEAYAE
                     RRGLGPEWVANGLWRWKRVPPSIMEGLERAVPGITLVREGAPRSGEGNLRLRLQDGFS
                     PCILGFSIEGAFSRDLDLERVSSVLNMVGAVEGGPKEGWCAVGNVTVFREGALIAKGA
                     DQERIRRDVERVRRAVVKAEECVGCGVCIARCKEGALLLMQGKVRVEAVRCVHCGECM
                     EPCPAISFGDAAFDY"
     gene            complement(2936..>3216)
                     /locus_tag="PLJ11_06085"
     CDS             complement(2936..>3216)
                     /locus_tag="PLJ11_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO04275.1"
                     /translation="VGEAQRVAEIVTNSVSPRARIIWGCSIDPELEGVVKVLLIITGA
                     KSRYIMGSRGAPIGMQSYQQQYQPQPQYQQQYQQPRPRNRDEDIDFVR"
BASE COUNT          531 a          949 c         1125 g          611 t
ORIGIN      
        1 gcctgcttgt gcaccgcctc ctctatctcc tccaattggc ctgtggtatc gtggaaggcc
       61 gcatacccca gcctccagcc gggaagcaga tccaccttag agaagccgtt gaccaggatg
      121 accggcacgt ccttggcgag ggtggcgggg gagtgatgct caccctcgaa ggtcatcagg
      181 tcgtagatct cgtcggagat gatgaacagg ccgaactccc cggccaggtc agcgatctcc
      241 ttcagccgtt tcgccgtgta caccgccccg gtggggttgt tgggattgat gaccgcgatg
      301 aacttggtgc gcggcgttat cttcttcctc aggtcatcga tgtccggttg ccagccctcc
      361 tcctccatgg tccggtaggt gatcgccttc ccgccgaaga acttcgggaa ctcgaggtac
      421 gaggtgtagc ttggtcctgg gaccagtgcc tcgtccccaa cttcgatggt ggccgcacag
      481 accatctgca gtgcctcggt cacgccgttg gtcacgatca tgtcctttag ggctagatcg
      541 atcctgttct tcttcctctc cttctcgata atcgccttcc ggagctcgag gttgccctcg
      601 gactcctcgt agccattgtc gcacttctct acggcagcgc agagggcgtc ccgtacgtac
      661 ttcggtgtct tgaaatcgaa cttgttgggg tcgccgatat gcagcttgat gatcctgtgg
      721 ccctgcttct ccagctccct tgctggaagc agcatctccc ggatcgcata gctgataccc
      781 atcgtgcgag cggtggcttt catggtatca ggcatggtat caggcgggat gatgagattc
      841 ctgctatatt agccttcgat tccctgacat tatgatgggg gagcggccac gtccgtcccc
      901 ttgccccaag acgacatttt tataggggtt cctgcagtag ccgcaggcat ggccgaggtc
      961 cggctaggga agatgcacct ccggtggtgc gacagctgcc atgtccccgt ccttgaggac
     1021 gcccagtgcg ggagatgcgg gacggccacg aggcaggtgg agctgacgcc ccccggggac
     1081 gcccggcctg cgttcgccta tgacctggag agggcgcgtg gcctcctgga ccggcagttc
     1141 ggggaggggt cgggcatcgc cctgctgcct gaggggagcg tctacctgat gaacagggcc
     1201 ccggccttgg accgcatgga cgaggtaatc gcggacgggg aggtggtggc gaccctacgc
     1261 tatgacctgg gcgtaggatg gagggccctc ccgcgggtga ggggcgccgc catgatcgtc
     1321 cgaacggcca gcaggaatct cgtctgggcg gaccaggggg cggtcgcgcc catcctggcc
     1381 tcgcagaacc ttatggcccc gggggtcgca aaggtccagg gcgacttcca acggggggag
     1441 gaggtcatag tgctcgaccc ccacggaaac gcattggcca ccggcgtggc ccgcatgtcc
     1501 tcccaggaga tgcgcggggg tggacggggg gtggcggtca aggtgcggtg ggctgagagg
     1561 ctcgaggccc ccccggagag agggcgggcc gattggggcg tggccctgga ggcgaacgcc
     1621 cgggtcatgg agcgaagggt cagggagagc gtcgggttca tcaggaggct ggtggaggag
     1681 catggagacc tgccgaaggt ggtctccttc tccggcggca aggactcact ggcgaccctc
     1741 ctcctcacca gggaggcggg cctggacctc cccgtcctct tcatcgacac gggactggag
     1801 ttcccggaga ccgtggagta cgtccatcgc ctgaaggagg agcttggcct ggacctggtc
     1861 gtaggaaagg cggacgagga ggccttcttc aagaacgtgg agttcttcgg ccccccggga
     1921 aaggactacc ggtggtgctg caagaccaac aagctcgggc cgacggtcaa ggccattgtg
     1981 gaccattatc cggagggcgt cctgtccttt atcgggcagc gccgctacga atcggagcag
     2041 cgggccgcta agcccagggt ctggcgcaac ccatggacgc cggggcagat cggagcgtcg
     2101 cccatccagg attggactgc attgcacgta tggctccaca tcatgcggag cggggtgagc
     2161 tacaatccct ggtacgagcg cggcctggac cggatcggct gcttcctttg cccggcctcg
     2221 gacatggctg aactggagat cgcccgctcc agcccctcgt acgaacggtg ggaggccttc
     2281 ctcgaggcct acgccgagcg ccggggacta ggcccggaat gggtggccaa tggcctatgg
     2341 cgctggaagc gggtgccgcc gtcgatcatg gaggggctgg aacgcgccgt accgggcatc
     2401 accctcgttc gggagggggc gcctaggagc ggggagggga acttacggct gaggcttcag
     2461 gatgggttct cgccctgcat cctgggattc agcatagagg gggcgttctc gcgcgacctg
     2521 gacctggaac gggtaagcag cgtcctgaac atggtgggag cggtggaggg agggccgaag
     2581 gagggatggt gcgcggtcgg caacgtcacg gtcttccgcg agggggcgct gatcgcgaag
     2641 ggggcggacc aggaaaggat aagaagggac gtggaaaggg ttcgccgtgc ggtggttaag
     2701 gccgaggagt gcgtcgggtg cggggtgtgc atcgccagat gcaaagaggg tgcacttttg
     2761 ctgatgcagg ggaaggttcg cgtggaagcg gtccgatgcg ttcattgcgg tgaatgcatg
     2821 gagccctgcc ccgcgatcag ctttggcgac gccgccttcg actactagcg gccattggac
     2881 cgcgcggcgg ccaatggtcg agtgaacggc gcagccctcg ttggccccct ccgcatcagc
     2941 gcacgaagtc gatgtcctcg tcccggttcc gcggccttgg ctgttggtac tgctgctggt
     3001 attggggctg cggctggtac tgctgttggt agctctgcat ccctatcggg gcgccgcggc
     3061 tgcccatgat gtacctggac ttggccccgg tgatgatgag caggaccttg accacgccct
     3121 ccagctcggg gtcgatggag cacccccata taatgcgcgc ccttggggac acgctattgg
     3181 tgactatctc cgccaccctc tgggcctccc cgaccg
//
LOCUS       DAOSYN010000150         3182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_23989_length_3182_cov_5.195395, whole genome shotgun sequence.
ACCESSION   DAOSYN010000150 DAOSYN010000000
VERSION     DAOSYN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23989_length_3182_cov_5.195395"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..2074
                     /locus_tag="PLJ11_06090"
     CDS             92..2074
                     /locus_tag="PLJ11_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOO04276.1"
                     /translation="MSPAALPSLMLSLPLLGAVLAVLLRSERSRVYSYLCSAASAVAA
                     AIWSVQVLLEGTASLVSDAPSVWGEYAIRLDPLGALVLLITALTFACVAMYALFSREG
                     VTARSALLFNCFVLSILLAMVADHVLLLMIAWEAMSLTSFLGAWGAREDEDTIAGWRY
                     LAITHFGAALVIAALAYLAVSSGEVRLSEMRGLSALIGPGASAAVVIMLFLGFGSKLG
                     LLPFHIWMPDLYSRSSSTVTALLSTAASNVAILVLVRVTFSCVGVEGQYALSLTVMGL
                     AALTALWASWQALVQRDGARVLAFSSMENMGLILLTMGAAMLFRAYGAAEPAAFALMA
                     ALFHLINHTVFKALLLMGAGTVRRAAGEWDLERLGGLARGLPGLSAVFLIGTLSVAAL
                     PPFNGFVSEWAMFKALMHAEPIADPLVRIALPLAIAVMALCTAIAATAYLRLYGLTFL
                     GRPRSAGAERPRPLERSELFPLLPLALLCILLGVASFPLLEELAAAVSPLVGASGLKV
                     VEGSSINFLHQGGLDPLGLSALLGFGMLVAFLIVRATGRRKVERVDTWDCGTPLDRRT
                     QYTPTGFAQPLLRVFQKVYRPQIEVREEPRPSEYIRAVRFEQRIPEPFVDHLYKPLTA
                     GAIALAMRVKRMQSGSIQAYLTYIMVTLVVLLVALR"
     gene            2079..3020
                     /locus_tag="PLJ11_06095"
     CDS             2079..3020
                     /locus_tag="PLJ11_06095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOO04277.1"
                     /translation="MQLTDVLIVIAEVSFVLMVSPLLTGIIGKAKAWMQCRQGPSVLQ
                     PYRDLWKLMRKATVASREASWLFFVIPYVCLGAMICLAVMVPVFNVGPSGGVGDMVAT
                     IYVLGLFRFMMVLGGLEGGSPFGGMGSSREMMISAIVEPTLLLSIFSVAAISGTTDLS
                     SISRFFAALGPKGLSPTLFLASAAIFISLLAENSRVPFDNPATHLELTMVHEGMLLEY
                     SGRELAMMEMASYVRLTMFMVILSNVFFPWGIALGTGAGELLVGVASISLKLLLLSVS
                     VAVVESVMAKMRLFRLPNLLTVSFTLSLLAVMSYYIL"
BASE COUNT          434 a         1118 c         1000 g          630 t
ORIGIN      
        1 atgaggcttt ccatggtgcg gcgcccatcg agcgcacaag aggcaaggat gtctcccttt
       61 atctgctcca aagcccattg aggccaagcc catgtcccca gccgcgctgc cctccctgat
      121 gctctccctc cccctcctcg gggcggtcct agcggtcctg ctacgaagcg agcggtcccg
      181 ggtgtacagc tacctgtgct cggccgcatc ggcggtggcg gcggccatat ggtccgtgca
      241 ggtcctcctc gaggggacgg cctcgctggt ctcggacgct ccctcggtct ggggggagta
      301 cgccatccgc ctggacccgc tgggcgccct ggtgctcctg atcaccgcgc tcaccttcgc
      361 ctgcgtcgcc atgtacgccc tgttctccag ggagggcgtg acggcccgga gcgccctgct
      421 gttcaactgc ttcgtcctgt ccatcctcct ggccatggtg gccgaccacg tgctgctcct
      481 catgatcgcc tgggaggcca tgtccctgac ctccttcctg ggggcatggg gcgcccggga
      541 ggatgaggac accatcgccg gctggaggta tctcgccatc acccatttcg gggcagccct
      601 ggtcatcgcg gccctcgcct atctcgccgt ctcctccggg gaggtccgcc tgagcgagat
      661 gcggggcctc tccgctttga tcggcccggg cgcatcggcg gcagtggtga tcatgctctt
      721 cctgggattc ggaagcaagc tcggcctgct ccccttccac atctggatgc cggacctcta
      781 cagccggtcc tcgtccaccg tcaccgccct gctgtccacg gccgcctcca acgtggccat
      841 actggtgttg gtgcgggtca ccttctcctg cgtcggggtg gaggggcagt acgccctttc
      901 cctgacggtg atggggctgg ccgccctgac cgccctgtgg gcctcctggc aggcgctggt
      961 ccaaagggac ggggccaggg tcctggcctt ctccagcatg gagaacatgg ggctcatcct
     1021 gttgaccatg ggcgccgcca tgctcttcag ggcctatggg gcagcggaac cggcggcctt
     1081 cgccctcatg gccgccctat tccacctgat caaccacacg gtgttcaagg cgctgctgct
     1141 aatgggcgca ggcacggtgc gcagggcggc gggggaatgg gacctcgagc ggctcggagg
     1201 cctcgcgaga ggtctccctg gcctctccgc ggtcttcctc atcggcaccc tctcggtcgc
     1261 cgcgctcccc cccttcaacg gcttcgtcag cgagtgggcg atgttcaagg cgctgatgca
     1321 cgcggagccg atcgccgacc ccctcgtccg gatcgcgttg cccctggcca tagcggtcat
     1381 ggccctatgc acggccatcg ccgcgaccgc ctacctgcgc ctgtacgggc tcaccttcct
     1441 gggccgcccc cgctcggcgg gggcggagag gccgcggccc ctggagcgca gcgagctgtt
     1501 ccccctgctc cccctcgccc ttctgtgcat cctcctgggc gtggcctcct tccccctgct
     1561 ggaggagctc gccgcggcgg tgtcgccgct ggtcggcgcc tcaggattga aggtggtcga
     1621 gggctccagc atcaacttcc tccaccaggg cgggctggac ccgttggggc tgagcgccct
     1681 cctgggcttc gggatgctgg tggcgttcct gatcgtaagg gccaccggca ggcggaaggt
     1741 cgagcgcgtg gacacctggg actgcggcac gcccctggac cgccgcaccc aatacactcc
     1801 aacagggttc gcccagcccc tgctccgggt cttccagaag gtataccggc cccagatcga
     1861 ggtcagggag gaacccaggc cctccgagta cataagggcg gtgcggttcg agcagcgcat
     1921 ccccgagccc ttcgtggacc atctctacaa accccttaca gcaggggcca tcgccctggc
     1981 gatgcgggta aagagaatgc agagcggtag catccaggcc tatctgactt atataatggt
     2041 caccctggtc gtgctcctgg tggcgctgag gtgatggcat gcagctgacg gacgtactca
     2101 tcgtcatcgc ggaggtgtcc ttcgtcctca tggtctcgcc cctcctcacc ggcatcattg
     2161 gcaaggccaa ggcctggatg cagtgccggc aagggccaag cgtcctgcag ccctaccgcg
     2221 acctgtggaa gctgatgcgg aaggccacgg tggcctcgcg cgaggcctcc tggctcttct
     2281 tcgttatccc atacgtctgc ctaggggcga tgatctgcct cgcggtcatg gtgccggtct
     2341 tcaatgtcgg cccctcgggc ggggtcgggg acatggtggc gaccatctac gtgctcgggc
     2401 tcttcaggtt catgatggtc ctcgggggcc tcgagggggg aagccccttc ggcggcatgg
     2461 gcagcagccg ggagatgatg atatcggcca tagtggagcc gaccctcctg ctctccatct
     2521 tctccgtggc cgccatatcc gggaccaccg acctgtcgtc catatccagg ttcttcgcgg
     2581 ccctcgggcc gaagggcctc tcccccaccc tcttcctggc ctccgccgcc atcttcatat
     2641 cgttgctggc cgagaactcc cgcgtcccgt tcgacaaccc agccacccat ctggagctga
     2701 ccatggtgca cgagggcatg ctcctggaat attccggaag ggagctggcg atgatggaga
     2761 tggcctccta cgtccgcctg acgatgttca tggtcatcct gagcaacgtc ttcttcccgt
     2821 gggggatcgc gctcggcacc ggcgccggcg agctgctcgt cggcgtagcc tccatctccc
     2881 tgaagctcct cctgctctcc gtcagcgtgg cggtggtgga gtcggtgatg gccaagatgc
     2941 gcctgttccg gctgccgaac ctgctgacgg tctccttcac gctatccctg ctggcggtga
     3001 tgtcctatta cattctgtga ggtgagagaa cggccgacct gctcagcata gacaccatca
     3061 acttcctggc cgcggccatc ctgctggtct ccctgctggt catggcgagc acccgcatgg
     3121 gccagctcgt ccgttccttc gccttccaat cgctgatgct ggcggccatc gccgccgtgc
     3181 tg
//
LOCUS       DAOSYN010000151         3180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_24012_length_3180_cov_3.483200, whole genome shotgun sequence.
ACCESSION   DAOSYN010000151 DAOSYN010000000
VERSION     DAOSYN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24012_length_3180_cov_3.483200"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..469)
                     /locus_tag="PLJ11_06100"
     CDS             complement(161..469)
                     /locus_tag="PLJ11_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04278.1"
                     /translation="MLKCSKCGAELDDESGGPLASMSGSFLGDEWTESYFFCPDCGTY
                     TLEIIHDCFLDQETSSVQGPITKEKGDALVELIRRCPEPWDKKCRCPAHLEYFQGQLD
                     "
     gene            complement(564..1076)
                     /locus_tag="PLJ11_06105"
     CDS             complement(564..1076)
                     /locus_tag="PLJ11_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO04279.1"
                     /translation="MVSDEDIKTAIPDFIIRYAQPKDVPMVLDFIRRLAEYERLSEEV
                     VATEEVLMDFLFNKRQAEVIIGELNGKPVAFALFFHNFPTFLGKANLYLDDLFVDESC
                     RGLGLGKVMFACLARIAVDRGCERLDRWCLDWSEPSISFYKGLGAEPMSDWTVYRLDG
                     EQLRKMAESR"
     gene            complement(1192..1266)
                     /locus_tag="PLJ11_06110"
     tRNA            complement(1192..1266)
                     /locus_tag="PLJ11_06110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1229..1231),aa:Ile,seq:gat)
     gene            complement(1458..1910)
                     /locus_tag="PLJ11_06115"
     CDS             complement(1458..1910)
                     /locus_tag="PLJ11_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HOO04280.1"
                     /translation="MKYTERALPQMYTMSPRAGKLLNDEEAMEKYVKEDQWGMATAVD
                     LSHCDPEKIRSKEVITQFAIDLCEYIKMKRFGDPIVVRFGPTPKVEGYSLAQLIETSM
                     ISGHFAEDTNRAFIDIFSCREYPPKKTAEFCKRYFGAKEMEYSVTFRN"
     gene            2398..>3180
                     /locus_tag="PLJ11_06120"
     CDS             2398..>3180
                     /locus_tag="PLJ11_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HOO04281.1"
                     /translation="MSPTDGLRNKGRTRADMEDRRRAVEERTGVRLEHSSNCSFDPLL
                     AEKNVENMIGCTQVPLGFVGPLRVNGDHAQGEFLVPMATTEGALVASVNRGASVITAS
                     GGAEALLVRDEMTRAPVFRTDGVRHSQKVVAWVNSNFDELVAAVASTTGHGKLVRVRP
                     FASGRSLFLRLSFETGDAMGMNMCTIASEAACRLIEERTGAKLISVSGNMCVDKKPAA
                     LNFIEGRGKTVLADVRVPARSVEERLHTNREAMVEDCYRKNLI"
BASE COUNT          674 a          930 c          899 g          677 t
ORIGIN      
        1 gtatacgagt tcgggctgcc ccgcctgacc gaggagttgg atgacgcgac catagccgaa
       61 tacgccaaac tgctggtgca agaggacaag tacttcgcgg aaatgttcgg cgagctcacc
      121 gcctggatga acggcctgcc gcagctacct aaatgaacgc tcaatccagc tgtccctgga
      181 aatattccag gtgcgccggg catcggcatt tcttgtccca tggttcaggg catctgcgga
      241 tcagttccac cagggcgtcc cccttctcct tggttatcgg cccctgaacc gaggaggtct
      301 cctggtccag gaaacaatcg tggatgattt ccaaggtgta tgtgccgcag tctgggcaga
      361 agaagtagga ctcggtccac tcgtctccca ggaaggagcc ggacatggag gccaaaggtc
      421 cgccactctc gtcatcaagc tcggcgccgc acttggagca tttgagcatg cagatcccgt
      481 tgcatatacg tgttagtaag aatgaccttt ttcctcggga gtctccgtcg cccgtgttcc
      541 gcgtcccggt cgatgagcgc atctcagcgc gattctgcca tcttcctcag ctgctcgccg
      601 tccagacgat agacggtcca atcagacatc ggctcggctc ccaagccctt gtagaacgag
      661 atgctcggct cgctccagtc caggcaccac cggtccagcc gctcgcatcc cctatccaca
      721 gctatcctgg ccaaacaggc gaacatgacc ttgcccagtc caagaccacg gcacgattcg
      781 tcgacgaaca ggtcatccag gtacaggttg gccttgccca ggaacgtggg gaagttgtgg
      841 aagaacaggg caaaggcgac cggtttcccg ttcagctcgc cgatgatcac ctccgcctgg
      901 cgtttattga aaaggaagtc catcaggacc tcttcggtgg ccacgacctc ttcggacagc
      961 ctctcgtatt cggccaacct tctgatgaag tccaggacca tcggcacgtc cttgggctga
     1021 gcgtaccgga tgatgaagtc cgggatggcc gttttgatgt cctcatccga gaccatgtga
     1081 acgggcggtg agggtttcgc tcgttaaaac aattaactag gcctatcaag gggaaatgga
     1141 gaaaaggctg ccgtcaacgc gaacgcgcga tcggtcaagc cagtgaatgg atgggcccaa
     1201 ggtgattcga acacctgacc tcccggttat cagccgggcg ctccaaccag gctaagctat
     1261 gggcccgagt gaaggggcga atgcggcgtt caattttaaa cctttccatg gtcctggccg
     1321 gtcggcgtga ggaattaatg gggatgcttg ccgttcaggc cccgccgaaa gtaagtataa
     1381 ggtgaagcat ggcccagcca cggcatggcc gcagctgggc attaaaggat tttcggtcat
     1441 agagttcaaa cgggcgttca gttcctgaag gtgacggagt actccatctc cttggcgccg
     1501 aagtatctct tgcaaaactc ggcggtcttc ttcgggggat actcacggca actgaagatg
     1561 tctatgaacg cgcggttcgt gtcctcggcg aagtgaccgg agatcatcga ggtctcgatc
     1621 agttgggcca gtgaatatcc ctcaaccttg ggggtggggc cgaacctgac gactatggga
     1681 tctccgaacc ttttcatctt gatgtattcg caaaggtcga tggcgaactg ggttataacc
     1741 tccttgctcc ggatcttctc cgggtcgcaa tggctcaggt ccactgcggt cgccattccc
     1801 cattggtcct ccttcacata cttctccatc gcttcctcat cattcagaag cttgcccgcc
     1861 ctcggactca tggtgtacat ctgtggtaac gccctctcgg tgtacttcac cttgaatttc
     1921 ccgcataaac gcttctgata ttaaaacata acccgtaact atatgtaatt ttaaaaagaa
     1981 ttttgagcgt acaaaattat ttttaattca ttgtaaagca atgacattta ttttatcgcc
     2041 ctgttctata ttaactaatg cgaaccttct gccctgccca tggccccaaa ggggaacggc
     2101 gcagtccttc ccgggccttg tcatcgagcc ttcctcacct ctttggggca tcttcaattc
     2161 ttcggttccc aatccaatgg gcgccgactg aggaccgacg gacatggaag gccgtcctcc
     2221 atcatagcac gtccccatgc ataggtccgg gaaaaggggt ctccagcaat ggcaatccgg
     2281 aaggtcacag ggctgaccgg gggcgacgga catcagcaac cgctcctccc cgcttcccga
     2341 tcggacctcc cttgcccggg cgagaaggtt atctaatacc atcctgattc cttgccaatg
     2401 agccccaccg acggtcttag gaacaagggc aggacccgcg cggacatgga ggaccgccga
     2461 agggcggtcg aggaacgcac cggcgtccgt ttggaacatt cgagcaattg ttcgttcgat
     2521 cccctcttgg ccgagaagaa cgtcgagaac atgatcggct gcacccaagt gcccctgggc
     2581 ttcgtcggac ccctgagggt gaacggggac cacgcccaag gggagttcct ggtgcccatg
     2641 gccaccacgg agggggctct cgtggcctcg gtgaacagag gcgcttcagt gatcaccgcc
     2701 agcggagggg ctgaagcgct tctcgtaagg gatgagatga cccgcgcgcc cgtgttccgg
     2761 acggacgggg tgaggcattc ccaaaaggtc gtcgcttggg tgaactccaa ctttgacgag
     2821 ctggtggcgg ccgtggcgtc cacgaccggc cacggaaagc ttgtcagagt caggcccttc
     2881 gcctcaggcc gttccctctt ccttcgtctt tccttcgaga ccggcgacgc gatggggatg
     2941 aacatgtgca ccatcgcctc cgaggcggcc tgccgcctca ttgaggaaag gaccggggcc
     3001 aagctgatct cggtctcagg caacatgtgc gtggacaaga agccggccgc cctgaacttc
     3061 atcgagggca gggggaagac cgtcctggcc gatgtccgcg ttcccgcacg ctcggtggag
     3121 gaaagactgc atacgaaccg ggaagccatg gtggaggact gctatcgcaa gaacctgata
//
LOCUS       DAOSYN010000152         3176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_24051_length_3176_cov_4.329702, whole genome shotgun sequence.
ACCESSION   DAOSYN010000152 DAOSYN010000000
VERSION     DAOSYN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24051_length_3176_cov_4.329702"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLJ11_06125"
     CDS             <1..836
                     /locus_tag="PLJ11_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04282.1"
                     /translation="CCIKLYTLSDSTKDMWEDNFYSMSEAVRSHGRVICLDDPNRPLE
                     ALYEPATKTAFLFNFDYYGWIKSVALAIAGDILEDEHQIYSVHGAALDMDGKGVTLIA
                     PSKTGKTTHSWGLLRSKDARLVTDDWYYVRLSERRPVAFGSEKNCYIDADIGTIWEEY
                     RGLVERAKLDNRKRAVVNVRWVTGKGSVVPMTTIYDIVLLKRDRDDPRLVRELGPDEA
                     WEHLAANDLCNPHQLVRDERKMRVRERFFRQFLAKSRVHLVNTTASPQETQAAIRQLL
                     G"
     gene            970..1443
                     /locus_tag="PLJ11_06130"
     CDS             970..1443
                     /locus_tag="PLJ11_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04283.1"
                     /translation="MGKVLITMTGRSVWGLFNSAWASIRQFDFLPDKVYVLTAGCGTD
                     RGRMVREMLTVLLREHGSPAEIELLEVPEDDIKEIGRLARETVERENALGNEVAIDVT
                     PGTKATVLGTIIGAGKGRVDHIFYLHIDSLRNASRPYLEIPLGLQRSHDVRREVL"
     gene            1443..2546
                     /locus_tag="PLJ11_06135"
     CDS             1443..2546
                     /locus_tag="PLJ11_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04284.1"
                     /translation="MEVDKDFITVVLNEALMAGKRKVVIRSDLMETDLLAVDLDRQEV
                     QLVVQEDVYDEALSRMARAVGDRLAMDLPNYLDLREALQASLLLPPSNLDGLMAEIYR
                     VTERRGDPYRFPRQMCFGLDTNLLYRRLFSRLLLAGTRCGVRPFDPQKVQLLIPSMAE
                     EELSRRVGRKYDKRDVEELKRALRDRSVAEELFNCLDKGGRKALNGQAEVAALRQRYS
                     CWSAQGGSFVEDKEVRDDEMLGSVADEMLEQRVDVLFLTNDDKTRAHANAHKVPSLPI
                     SYPYEVPSTIQYDPWLLTELVYDLSILHTSLTLKGTGVRIRGEWMGKTVDDYRSERVR
                     VIALQGSEIGEMLARDMRVISAIKSRFPWKELV"
BASE COUNT          671 a          873 c         1064 g          568 t
ORIGIN      
        1 ggtgctgcat caagctgtat accctgagcg attcgaccaa ggacatgtgg gaggacaact
       61 tctactcgat gtccgaggcg gtgcgctcgc atggccgcgt gatatgcctg gacgacccga
      121 accgcccgct ggaggcgctt tacgagccgg ccaccaagac cgcgttcctg ttcaacttcg
      181 actattacgg gtggatcaag agcgtggccc tggccatcgc aggggacatc ctggaggacg
      241 agcaccagat atattcggtg cacggcgcgg ccctggacat ggacggcaag ggcgtgaccc
      301 tcatcgcccc gtccaagacc ggcaagacca cccattcctg gggcctgctc cgctccaagg
      361 acgcccggct ggtcaccgat gactggtact acgtccgcct gtccgaacgg cgcccggtcg
      421 ccttcggctc ggagaagaac tgctacatcg acgcggacat cggcaccatc tgggaggagt
      481 accggggatt ggtggagagg gcgaagctcg acaaccgcaa gcgggcggtg gtgaacgttc
      541 gctgggtgac cgggaagggc tcggtggtgc cgatgacgac gatctatgat atcgtgctgc
      601 tcaagcggga ccgcgacgac ccccggcttg tccgggagct ggggcctgat gaggcctggg
      661 agcatttggc ggccaatgac ctttgtaacc cccaccagct ggttcgggat gaacggaaga
      721 tgagggtgcg ggagcggttc ttcaggcagt tcctggccaa gtcgagggtc catctggtga
      781 acacgacggc ctccccccag gagacccagg ccgccatccg gcagctgctg ggatgaggag
      841 gcgtggggga gggaaacggg gaagccctgg gtcccctgtc aggccatgga ccgtttgccc
      901 tcgaacgccg cctccatcgc cgggcgaatg gaggaaaggc tgattacccc tccatcccct
      961 ggactaggca tgggcaaggt cctgatcacc atgacggggc gcagcgtctg ggggctgttc
     1021 aattccgcct gggcgtcgat ccggcagttc gatttccttc cggacaaggt ctacgtcctg
     1081 accgccggct gcgggacgga cagggggcgg atggtccggg agatgctgac cgttctgctg
     1141 agggagcatg gatcgccagc ggagatcgag ctgctcgagg tccctgagga cgacatcaag
     1201 gagatcgggc gcttggcgag ggagaccgtt gagagggaga acgccctggg caacgaggtg
     1261 gcgatcgacg tcacgccggg caccaaggcc acggtgctgg ggaccatcat cggggcgggg
     1321 aagggcaggg ttgatcatat cttctatctg cacatcgatt ccctccggaa cgcttccaga
     1381 ccgtacctgg agatccccct gggcctgcag cgctcccatg atgtcaggag ggaggtccta
     1441 tgatggaggt cgacaaggac ttcatcaccg tcgtcctcaa cgaggcattg atggcgggca
     1501 agcggaaggt cgtcatccgc tccgacctga tggaaacgga cctgctcgcc gtggacctgg
     1561 accgccagga ggtgcaattg gtggtccagg aggacgtcta cgatgaggcg ctgtccagga
     1621 tggcgagggc ggtgggagac cggctagcca tggacctacc gaactaccta gacctgaggg
     1681 aggcgctgca ggcctccctg ctccttcccc cgagcaacct cgatgggctg atggcggaga
     1741 tctaccgcgt gaccgagcgc cgtggggacc cgtaccggtt cccccggcag atgtgcttcg
     1801 ggctagatac caatctcctc taccggcgtc tgttctccag actccttctg gccgggaccc
     1861 gctgcggggt acggccattc gacccgcaga aggtccagct gctcataccg tcgatggccg
     1921 aggaggaact ctcgcggagg gtgggcagga agtacgataa aagggatgtg gaggagctta
     1981 agagggcctt acgggaccgc agtgtcgccg aggagctgtt caactgcttg gacaagggcg
     2041 gacggaaggc cttgaacggg caggccgagg tcgcggccct aaggcaacgc tacagttgct
     2101 ggtcggcgca gggcggctcc ttcgtcgagg acaaggaggt gagagacgac gagatgcttg
     2161 gttccgtggc cgatgagatg ctcgagcagc gcgtggacgt gctgttcctg accaacgatg
     2221 acaagacgag ggcgcacgcc aacgcgcaca aggtcccgtc cctgccgata agctatccgt
     2281 acgaggtgcc atcgaccatc cagtacgacc catggctgct taccgagctg gtatatgacc
     2341 tcagtatctt gcacacctcc ctgaccctga agggtaccgg ggttaggatc agaggggaat
     2401 ggatgggcaa gaccgtggat gattaccgaa gcgagagggt gcgggttatc gctttgcaag
     2461 ggtcggagat cggcgaaatg ctggccaggg atatgcgggt gatctcggcg atcaaatcca
     2521 ggttcccttg gaaggagctt gtctaaggaa gggtgagggt aaaggagacc ccgcaaaacg
     2581 aaaatggtgg ggaagttgga gaaggtccgc ggggtgctcc taatatgtgg atggtcggtt
     2641 gactataaaa attacgctct aattgtatca tttctcagaa ataatcgatt gactaggaaa
     2701 acgccttcca gccgtcggca cccatttcaa ttccaacctg gcgtaaagag cgcgcgcatt
     2761 ggatttgagg gatggcggcc atcttccgtt cttagccgcc atgatcggcc taacgagcgc
     2821 ggggaccgct ggctctatcg cgatcacacg taaaaagaag gatggaccgc cctgtaccat
     2881 ttgaggctgg catggctggc cttcctcgat gctgcgagag agtgcgagag aaaccatgga
     2941 aatgaatccc ctaagatatc ctagttcgcg ggaggattga ccatagagga caccgggaag
     3001 caaccacgca tgccgttaag gaaggtggga agaggatgaa ggggcccatc cgatcctgga
     3061 aaggcatatg cccccatgaa cgcaccctaa tgaaaagttc gcaaataaga ggttggacca
     3121 ttaaaggaag tgggggcact gggatttgaa cccagatcag cgggtttcca ccacgt
//
LOCUS       DAOSYN010000153         3168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_24117_length_3168_cov_3.439447, whole genome shotgun sequence.
ACCESSION   DAOSYN010000153 DAOSYN010000000
VERSION     DAOSYN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24117_length_3168_cov_3.439447"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLJ11_06140"
     CDS             <1..253
                     /locus_tag="PLJ11_06140"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOO04285.1"
                     /translation="LTLLGLTAGFITTLGFIPQLLKGYRTGSMEDVSLTMPLVLMLGM
                     GMWLTYGVLTEDPPIIIWNAVSIVLNAGIVLLKVRSNGR"
     gene            complement(364..732)
                     /locus_tag="PLJ11_06145"
     CDS             complement(364..732)
                     /locus_tag="PLJ11_06145"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOO04286.1"
                     /translation="MTGDGWPWEESALPPEVAAGLREAGGREGLLAKMPSRRSLQASA
                     ERYQALSDPTRLLIMHLLGESVLCPCVLKDLTGLTDSKLSYHLSILERAGLVAWRQNK
                     NWRVYGLTALGRDCLKDRLP"
     gene            complement(789..1457)
                     /locus_tag="PLJ11_06150"
     CDS             complement(789..1457)
                     /locus_tag="PLJ11_06150"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOO04287.1"
                     /translation="MSRVSYSVIEVPTVSEELSRLEGLASAKGAVPARLSAKEVVVAE
                     WVRFKCRYGCKGYAKHLSCPPYVPGPRETRRMLDEYEHALLVRFDGVPGREGARPEDI
                     PEDFHPWYKGLILWVNRTVHLLEKTAFYDGFYKAFAFGAYPCIYCEHCVAEETEGTVD
                     ESVRRRCRHMDLVRPSMEAAGMDVLATARKVGWDIRPIPCKDMEYGKVTRMDLVSVGI
                     VLLE"
     gene            complement(1494..1757)
                     /locus_tag="PLJ11_06155"
     CDS             complement(1494..1757)
                     /locus_tag="PLJ11_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HOO04288.1"
                     /translation="MEIDPLPLVNLILCLAIVALGLLGYMRSRHTLPLILAVVFALFG
                     LSHAIILLDPSRELEVVVFAVRVVAYGLVLYLLYRYVHVLDIF"
     gene            complement(1953..2288)
                     /locus_tag="PLJ11_06160"
     CDS             complement(1953..2288)
                     /locus_tag="PLJ11_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04289.1"
                     /translation="MANRLRLTILSVACCQPQTAVHDKRYIELIRQVLAETGLEADID
                     LVTATEAQMSIHYFFIGEILPLFKKYGPAVAPALFVNERLTLFGGVPTAERLKEVLMK
                     AASEPPSES"
     gene            complement(2278..>3168)
                     /locus_tag="PLJ11_06165"
     CDS             complement(2278..>3168)
                     /locus_tag="PLJ11_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HOO04290.1"
                     /translation="EGRKFLFFGGKGGVGKTTMAATTATWLSDHGYKTLIVATDPTVS
                     LSAIYGQDISATEVTKIGTERNLCGLNINPKKAMGVFQSRLEGMMEGFSSMFGSELLS
                     TPCTEEIAAFDQFVSYFEDTEHDKVVFDTAPTGHTLRELSMPFDWSGYIANQIKNRRE
                     LSEVLGFVYDENMVADLEAEKARYDKSVKGLSDERISAFNLVLLPEKLPIEETARAIE
                     DLGGFGIKVRSLIVNEVIPPEVLKGNWFLERRRATQEKYLKEIAQRFPGIPRREVPLF
                     DTDIYGMEMLRMVGRELYGE"
BASE COUNT          580 a         1063 c          920 g          605 t
ORIGIN      
        1 cctcaccctc ctaggactga cggccggttt catcaccacc ctcgggttca tcccccagct
       61 gctcaagggc taccgtacgg gcagcatgga ggacgtctcc ctgaccatgc cgctcgtcct
      121 gatgctcggg atggggatgt ggctcacata cggcgtcctc acggaggacc ccccgataat
      181 catctggaac gcggtctcca tcgtcctcaa cgccggcatc gtgctgctga aggtccgctc
      241 caatgggcga tgagggcgtg gccagtctcc ccggggaaca ggcagcgcgg gtctagcatc
      301 ctcatgccgt tctcccctcc atcgtcccgc atcggcgcct gcggcattgg tccacggcct
      361 cggtcagggc aggcggtcct tcaggcaatc ccttccaagg gcggtcagcc catacacgcg
      421 ccagttcttg ttctgtcgcc aggccaccag acccgccctc tccaatatgc tcaggtgata
      481 ggagagcttg gaatcggtaa ggcccgtgag gtccttgagg acgcatgggc agagcacgct
      541 ctcccccagc aggtgcatta tcagcagcct ggtcggatcg gaaagggcct gataccgttc
      601 cgccgatgcc tggagcgatc tcctgctcgg catcttcgcc aggaggcctt cgcgcccacc
      661 agcctccctc agtccggcag ccacctctgg gggaagggcg ctctcttccc atggccaccc
      721 gtcaccggtc atctcccagc acttccaagg atgacagggg taagcgctgt tatcttacct
      781 tcggtccctc actccaatag gacgatcccg acggaaacca ggtccatccg ggtcaccttg
      841 ccgtactcca tgtccttgca cgggatcggc ctgatgtccc acccaacctt cctggcggtg
      901 gcgagcacgt ccatacccgc cgcctccatg ctcggccgga caaggtccat gtggcggcac
      961 ctcctgcgca cgctctcgtc cacggtcccc tcggtctcct cggccacgca gtgctcgcaa
     1021 tagatgcagg gataggcccc gaaggcgaag gccttgtaga agccgtcata gaaggcggtc
     1081 ttctccagga gatggacggt tcggttgacc cagaggatga ggcccttgta ccagggatga
     1141 aagtcctccg ggatgtcctc cggcctcgcc ccctcccggc caggaacacc atcgaagcgc
     1201 accaggaggg catgctcgta ctcatcgagc attctgcggg tctccctcgg cccagggacg
     1261 tagggcgggc aggagagatg cttggcatag cccttgcacc catacctgca cttgaagcgc
     1321 acccattcgg ccacaacgac ctccttggcg ctcagcctcg ccggcacggc ccccttggcc
     1381 gatgccaatc cctccaacct cgaaagctcc tccgagacgg taggcacctc gatcaccgaa
     1441 taggatactc ggctcaccgc tcattaaccc tgctacttcc gatcaatgtg cgatcagaag
     1501 atgtctagca cgtgcacgta ccgatagagc aggtacagca caaggccgta tgcgaccacc
     1561 cgtaccgcga agaccaccac ctcaagctca cgagaggggt caaggaggat gatggcgtgc
     1621 gacaggccga acagggcgaa cacaacggcc agtatgagag ggagggtgtg cctgctcctc
     1681 atatatccca acagccccag ggccactatg gccaggcaga ggatgaggtt gacaagggga
     1741 agcgggtcta tctccatggt gcaccgaatg tattcgttcg aatgtattcg ttcaaatcct
     1801 attttaaggg catgggtggg cgcttcgtcc cttcggtagg gtgagacggg cgatgggggc
     1861 agcttccaat ccctcatctg gacggagcta tcggccccca tcgaagcaat gccgcccatc
     1921 acaaggccct tgcagggccc cgcgctcctc tgtcagctct cggacggagg ctcggaggcc
     1981 gccttcatca ggacctcctt cagcctctcc gcggtcggaa cgcccccgaa caacgtcagc
     2041 cgctcgttga cgaagagggc cggggcgacc gccgggccgt acttcttgaa gagggggagg
     2101 atctccccga tgaagaaata gtggatgctc atctgggcct cggtggcggt cacaaggtcg
     2161 atgtcggcct ccaggccggt ctcggccagg acctgtctga tcagctcgat gtaccgtttg
     2221 tcatgaacgg ccgtctgggg ctgacagcag gcgaccgaca gtatggtcaa gcgcagccta
     2281 ttcgccatag agctccctcc ccaccatcct cagcatctcc atcccgtaga tgtcggtgtc
     2341 gaacagcggg acctcccggc gggggatgcc cggaaaccgc tgggcgatct ccttgaggta
     2401 cttttcctgg gtggccctcc gcctctccag gaaccagttg cccttgagca cctcgggggg
     2461 gatgacctcg ttgacgatga gcgaacgcac cttgatgccg aaccctccga ggtcctcgat
     2521 cgcccgggcg gtctcctcga tgggaagctt ctccggcagc agcaccaggt tgaacgcgga
     2581 gatgcgctcg tccgaaagcc ctttcaccga cttgtcgtac cgggccttct ccgcctccag
     2641 gtcggccacc atgttctcgt cgtacacgaa ccccagcacc tccgagagct ccctccggtt
     2701 cttgatctgg ttggcgatgt agccggacca atcgaaaggc atcgacagct ccctgagggt
     2761 atgcccggtc ggggcggtgt cgaagaccac cttgtcatgc tcggtgtcct caaagtagct
     2821 gacgaactgg tcgaaggccg cgatctcctc cgtgcacggg gtcgacagga gctcggagcc
     2881 gaacatggag gagaagccct ccatcatccc ctccaatctg ctctggaaca cgcccatcgc
     2941 cttcttgggg ttgatgttca gcccgcacag gttccgctcg gtgcctatct ttgtcacctc
     3001 cgtggcgctg atgtcctgcc cgtagatggc cgacaggctg acggtggggt cggtggcaac
     3061 gatcagggtc ttgtacccat ggtcggagag ccaggtggcg gttgtggccg ccatcgtggt
     3121 cttgcccact cccccctttc ccccgaagaa caggaacttc cgcccctc
//
LOCUS       DAOSYN010000154         3147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_24296_length_3147_cov_3.886805, whole genome shotgun sequence.
ACCESSION   DAOSYN010000154 DAOSYN010000000
VERSION     DAOSYN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24296_length_3147_cov_3.886805"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..684)
                     /locus_tag="PLJ11_06170"
     CDS             complement(256..684)
                     /locus_tag="PLJ11_06170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOO04291.1"
                     /translation="MSVFDFDASVPREARERLQRMMDIPFVQELGIRTVSISEGEVRV
                     AMDAGDKLNAVGGAHGGAVFTLADQAFALASNLEAWPQVALVASINYLRPGKGMLEAV
                     ARKTSETNRTSLYEVKVFDDGELIAVFQGTGYKLNGDRKG"
     gene            complement(1386..>3147)
                     /locus_tag="PLJ11_06175"
     CDS             complement(1386..>3147)
                     /locus_tag="PLJ11_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOO04292.1"
                     /translation="MPMNFFGRRRAFVSISVFLVVMAVISAGCAFLQIAGAEGDAACQ
                     VPVVPCLGGNALPGPPLGSIQGLTAYVEGEGEFTYDWRYDNDEYSVSFDLPDELYAYY
                     RDYSIQRYMATEGDRDNALNFITDNDSVVVYVAECMDDLSERVGLDDAGKAELVLAFV
                     QEIPYEYDDETHSMGDYWSFPVETLHDWVGDCEDTSFLYSSLMAALGYETALLVFEDH
                     IAEGVSGEEFDGWYYEIEGTRYYYCETTGTGWSIGEMPEEYDEAYVLPVGIRLGPPTD
                     LEAEVGDSEATISWSPPNSDGRSPVDRYAIYLDGEFVAEVYGTIYTLTDLLNGLEYEV
                     TIAAHNEEGYGPPAVLFIMPLAQGVLPEAPTALKVKAGDGAVTLTWSPPEDEGETAVD
                     FYVVFLDGRKVALVENTTYTIRGLMNGEEYEVTVAAHNSAGVGPKAPAVVVIPAPDEV
                     LPSWAIPLMLLILVTVIVIVCAVAVQTRRSRRMRAQMMQGTLQMGGDSAPPVPLPYQS
                     RVEGVRSEQPQGAPDTGPVVEGASIAMGSAPAAVVAPEASGDAQVDGKGLEPPRFCAY
                     CGSPLERDAVFCSNCGNRVR"
BASE COUNT          637 a         1030 c          901 g          579 t
ORIGIN      
        1 ttcccacgga tcggatgccc atgggagtat ttcaacgcat ctcaatgagg gtggcgcgac
       61 ggacggggcc gcccccaagg cctcagcctt aaaaaagagg caggccattg gcagcccgtc
      121 ccacgcgtgc tcgccaacga gccccttccg gtcatgcccg tctaggcgtt ccaagcggtg
      181 catcgccctg ggagaacagg gcgtaggggc cagtcccgat gcgacgatgc cctccgccca
      241 gtaactgaaa ggggctcagc ccttccggtc gccgttgagc ttgtaccctg tcccctggaa
      301 gacggcgatg agctccccgt catcgaacac cttcacctcg tagagcgacg tcctgttcgt
      361 ctccgaggtc ttccgggcaa cggcctccag catgcccttc cccggccgga ggtaattgat
      421 gctcgcgact agggcgacct gcggccacgc ctccaggttg gaggcgaggg cgaaggcctg
      481 gtcggcgagc gtgaacaccg caccgccatg cgcccctccc acggcgttca acttgtcgcc
      541 ggcatccatt gccaccctga cctccccctc ggagatcgag acggtcctaa tgcccagctc
      601 ctggacgaac ggaatgtcca tcatcctttg caggcgctcg cgcgcctccc ttggcaccga
      661 ggcgtcaaaa tcgaagactg acacaatgac cgaccttgaa tggcgaggag ggatttaacc
      721 ggtttggccg gagaacgaaa ggaaggcatc ccatgatggg ccttccgttc caacgttgat
      781 gaaacgaatc gctgcgggag atcaccgtcc ccgtgggcat ggcggagcat gcacggtgtc
      841 gaaggccgcg ccgccccagg gatctccggt gatgggaggg gcatcggccc acggcaattg
      901 gcatgggtgg cacgggtgcg atcaaatcaa ggcacgatgc cggtcccaag cccttgctaa
      961 tgcgcatgag accgggccga tggccgaaga caaagatccg caagcgcgat taatgcgccc
     1021 caggctttaa tgcctttagg acataaaatc gtcatggtag gttggatgga gctaaaagag
     1081 atcggtggat tcccagttcc agaggtgaca acatggctaa gaaccccaag aaggccgaga
     1141 aggttgccaa ggacgctacc cagaagaact gatcgcgctc cgtcaccgta tattgatgtg
     1201 agcgatcacg tcaaaccctc tttcgaattt tcatcggcgc ccaggaacgc ctgggcgctt
     1261 tatctgatag atgttagagg ggcggcggct ccgatctaaa agccccgctg agggggttcc
     1321 acgacgcagc gggcccttcg cacatgagcc gggatgggaa aggaagggcc cctcgaagag
     1381 acctctcacc tgaccctgtt gccgcagttg gagcagaaca ccgcatctct ctccaggggc
     1441 gagccgcaat aggcgcagaa gcggggaggc tccaggcctt tgccgtcgac ctgcgcatcc
     1501 ccgctcgcct ccggcgcgac gaccgccgct ggggcgctcc ccatcgctat ggaagcgcct
     1561 tctacgaccg gcccagtgtc cggagcgccc tgcggctgct cggaacggac cccttcgacc
     1621 cggctctgat aggggagggg aacgggcggg gcggaatcac cgcccatctg caaggtcccc
     1681 tgcatcatct gcgcccgcat gcgccggctg cgtctcgtct gaacggccac cgcgcatact
     1741 ataactatga ccgttacgag tatcaacagc atcaagggaa tggcccatga gggcagcact
     1801 tcgtccggag ctggaatcac gaccaccgcc ggggcctttg gccccacccc tgcggagtta
     1861 tgcgccgcga cggtgacctc gtactcctct ccgttcatca gcccgcggat ggtgtaggtg
     1921 gtgttctcga ccaatgccac ctttctcccg tccaggaaca cgacgtagaa atccaccgcg
     1981 gtctccccct cgtcctctgg cgggctccac gtcagggtca ccgcgccatc ccccgccttc
     2041 accttcaatg ccgtcggcgc ctccggtagc acgccctggg ccaacggcat tatgaataaa
     2101 acggcaggag gcccgtaccc ttcttcattg tgcgccgcga tggtgacctc atattccaat
     2161 ccgttcagga ggtctgtaag ggtatagatc gtgccatata cctcagccac aaattcgcca
     2221 tccaagtaga tggcgtagcg gtcaacgggg gatctcccgt cactgttggg cggggaccag
     2281 ctgatcgtgg cctccgaatc ccctacctca gcctccaggt ccgtcggagg tccgagccgg
     2341 ataccgaccg gcagcacgta ggcctcatca tactcctcag gcatctcccc gatcgaccac
     2401 cccgtgcccg tggtctcgca ataatagtac ctggtcccct ctatctcata gtaccatcca
     2461 tcgaactctt ccccgctcac gccctcggcg atatggtcct cgaagacgag cagcgcggtc
     2521 tcatacccca gcgctgccat cagcgatgag tagaggaagg aggtatcctc gcagtcccca
     2581 acccagtcat gaagggtctc caccgggaag ctccagtaat cgcccatgga gtgcgtttcg
     2641 tcatcgtact cgtatggaat ctcctgcacg aaggcgagca ccagctccgc cttccccgca
     2701 tcgtccaggc ctacccgctc gctgaggtcg tccatgcact cggcgacgta cacgacgaca
     2761 ctgtcattgt cggtgatgaa attgagggcg ttgtcccggt cgccctccgt ggccatgtat
     2821 cgctgaatgg aatagtcccg ataataggca tagagttcgt cggggaggtc aaagctcacc
     2881 gagtactcgt cgttatcgta tctccagtca tatgtgaact ctccctcgcc ttccacatat
     2941 gcggtcaggc cttggatgga acctaggggc ggtccaggga gagcgttccc accaagacag
     3001 ggaacgacgg gcacctggca ggcagcatcc ccttccgccc ccgcgatctg caaaaatgcg
     3061 cacccggcgg agatgacggc catcacgacc aagaagacgg atatcgatac gaatgctctc
     3121 cttctcccaa agaagttcat gggcatc
//
LOCUS       DAOSYN010000155         3108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_24662_length_3108_cov_5.210940, whole genome shotgun sequence.
ACCESSION   DAOSYN010000155 DAOSYN010000000
VERSION     DAOSYN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24662_length_3108_cov_5.210940"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /gene="trkA"
                     /locus_tag="PLJ11_06180"
     CDS             complement(<1..1284)
                     /gene="trkA"
                     /locus_tag="PLJ11_06180"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOO04293.1"
                     /translation="MHVIVVGAGNVGYTIARMLSKQHSVLVIEDDLKRYDYVVNHLNV
                     GALNGNGASPKVLKGVINEDTNLLLAVTERDETNIFCCMMAKQINPKLVTVARVRNPD
                     YIEGTMYSKFLNVDHIISPEYLTAVKLMRIAMFENAVGCESIPSLGVELAMFRVSGKR
                     EGVTMIPLRELPLPEECKVLVIHRGKQVIVPTPNDVLMKGDKVTIIGKGEAVSEFNRM
                     LGTVRDPRDIVIVGGGIVGEHLATLMEKEGLSVKLIENDEDRCRVLAKKLSRTVIIND
                     NGSDPSVLRNENVAMADVLICATETEEDNLLASLIGKHLGVSKVIATYSKREYEEIFG
                     MSGIDASVGYYHVVANEIIKETVHNYEVMLLLEKFSEEFFDLRVNAGSRVIGKKLGEI
                     DMPKMSSMALVVKDGKAIIPSSETVIEEGDKVFIYA"
     gene            complement(1548..1623)
                     /locus_tag="PLJ11_06185"
     tRNA            complement(1548..1623)
                     /locus_tag="PLJ11_06185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1587..1589),aa:Pro,seq:ggg)
     gene            complement(1646..1837)
                     /locus_tag="PLJ11_06190"
     CDS             complement(1646..1837)
                     /locus_tag="PLJ11_06190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HOO04294.1"
                     /translation="MIIPVRCFTCGKVVGSSYQTFVKRVQMGEDPQEVLDDLDIKRYC
                     CRRMLISNSDLLGELIPLG"
     gene            complement(1844..2245)
                     /locus_tag="PLJ11_06195"
     CDS             complement(1844..2245)
                     /locus_tag="PLJ11_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOO04295.1"
                     /translation="MSQVVNTSGKRKTAVARAVAREGTGMVRINSVPLDIVTPELARL
                     KMAEPLTLVPDKAAKVDIDVSVAGGGVMGQAEATRTAIAKAIVEYYQDEQLELAFKHF
                     DRSLLISDIRRKLPKKPLGRGARKKRQKSYR"
     gene            complement(2247..2666)
                     /locus_tag="PLJ11_06200"
     CDS             complement(2247..2666)
                     /locus_tag="PLJ11_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOO04296.1"
                     /translation="MVAVIDAENHIAGRLASIIAKRLLKGEEIVVVNAEKIVITGERA
                     SVLASYKQKRDCGNVKSGPFFPRRADLIMKRTVRGMLPWQTTSGKDAYRRLKVYVGVP
                     KEFADLQKEKVDVAIKTGLTQYVTLSQVAEFLGSKVK"
     gene            complement(2671..3030)
                     /locus_tag="PLJ11_06205"
     CDS             complement(2671..3030)
                     /locus_tag="PLJ11_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HOO04297.1"
                     /translation="MKAGKTDPNLAAVISALKAESRASGVGIWRDIALRLEKAKANWA
                     EVNLSKLERYASEGDVIVVPGKVLGAGELSKKVTVAAYGFSESARAKIEAAGGKDLTI
                     MELVKEMPKGTGVKIMG"
BASE COUNT          567 a          999 c          844 g          698 t
ORIGIN      
        1 agcgtagatg aagaccttgt caccctcctc gataaccgtc tcggaggagg ggatgatcgc
       61 cttcccgtcc ttgaccacaa gggccatgga ggacatcttg ggcatgtcga tctcccccag
      121 cttctttcct atgacccggg agcccgcgtt gaccctgagg tcaaagaact cctcggagaa
      181 cttttcgagg aggagcatga cctcgtagtt gtgcaccgtc tccttgatta tctcgttggc
      241 caccacgtga tagtagccga ccgaggcatc gatgccgctc atgccgaaga tctcctcgta
      301 ctctctcttg gagtaggtgg ctatgacctt cgataccccc agatgcttgc cgatcaggga
      361 tgccaacagg ttgtcctcct cggtctccgt ggcgcagatg agcacgtctg ccatggcaac
      421 gttctcattg cggagcaccg aggggtccga gccgttgtcg ttgatgatca ccgtccggct
      481 tagtttcttg gccagtacgc ggcagcgatc ctcatcgttc tcgatgagct tgacggaaag
      541 cccctccttc tccatgaggg tcgccaggtg ctctcccacg atcccgccgc caacgatcac
      601 tatgtcgcgt gggtcccgga cggtccccag catccggttg aactcggaca cggcctcccc
      661 cttgccgatg atggtcacct tgtcgccctt catcaggacg tcgttggggg tgggcactat
      721 gacctgcttt ccccggtgga tgacgaggac cttgcactcc tcggggaggg ggagctccct
      781 gaggggtatc atggtcaccc cctccctctt cccgctgacc ctgaacatgg ccagctcaac
      841 ccccaaggag ggaatgctct cgcaccccac ggcgttctcg aacatggcta tgcgcatcag
      901 tttgaccgcg gtaaggtact ccggcgaaat gatgtggtcc acgttgagga acttagagta
      961 catcgtgccc tcgatgtaat cgggattgcg caccctggcc acggtcacca gcttggggtt
     1021 gatctgcttg gccatcatgc aacagaagat gttcgtctcg tccctctccg tcaccgccag
     1081 gaggaggttc gtgtcctcgt tgatgacgcc cttcaggacc ttggggctag cgccgttgcc
     1141 gttcagagcg ccgacgttga ggtggttaac cacataatca tagcgcttca ggtcgtcctc
     1201 gatgaccagc actgagtgct gcttcgacag catcctggcg atggtatagc ccacgttccc
     1261 ggccccgaca acgatgacgt gcatccatca acgctcctca ttatcggccc ctaccgatcc
     1321 tttgctgact aatgcattcc ccattaagat atgtttgcca tccgaccggg gcctgcgagg
     1381 acgttcgaac cttagttcta ggcaggccat gatggcctct atattcacac agggtcagta
     1441 gggcggcggg catatgaact acgctgttca aggcccgccg ctttggatac aacggaatat
     1501 gcatgctgag ccattggtca ggaccaacgc aaggagcgaa gaaaagatgg tgggtccgtc
     1561 cgaatttgaa tcggagtcac ctgcacccca agcaggaagg attccaagct accccacgga
     1621 cccgttgtaa aggggtgaaa gaggtttaac ccagagggat cagctcaccg agcaggtccg
     1681 aattggagat gagcatccgg cgacagcagt accgcttgat gtccagatca tccagcactt
     1741 cctgggggtc ctcgcccatc tgcaccctct tgacgaaggt ctggtaggag ctgcccacca
     1801 ccttgccgca tgtgaagcat cttacgggga ttatcatgtc cgctcacctg tacgatttct
     1861 gcctcttctt cctggcaccg cggcccagcg gcttcttcgg gagcttccgg cggatgtcgc
     1921 tgatcagcag ggacctgtca aagtgcttga aggccagctc cagctgctca tcctgatagt
     1981 actccacgat cgccttggcg atggcggtgc gggtggcctc tgcctggccc atcacgcctc
     2041 cgccggccac gctgacatcg atgtcgacct tggccgcctt gtccggcacg agggtcaacg
     2101 gctcggccat cttcagccgt gccagctcgg gggtgacgat gtccaggggc acgctgttga
     2161 tgcgcaccat gccggtgccc tcgcgggcca ccgctcgggc caccgccgtc ttcctctttc
     2221 cactggtatt gacaacttgg gacatatcac ttcaccttcg agcccaggaa ctcggccacc
     2281 tgactgagcg tgacgtattg ggtcagccct gtcttgatag caacgtccac cttctctttc
     2341 tgcaggtcgg cgaactcctt cggcacgccc acatagacct tcagccttct gtacgcgtcc
     2401 ttgccggagg tggtctgcca gggcagcatc ccccgcacgg tcctcttcat gatgaggtcg
     2461 gcgcggcgag ggaagaacgg gccgctcttc acgttcccgc agtcccgctt ctgcttatag
     2521 gaggccagga cggacgcgcg ctcgccagtg atgacgatct tctcagcgtt gacgaccacg
     2581 atctcctcgc ccttcaacag cctcttggcg atgatcgatg caagcctgcc cgcgatatgg
     2641 ttctcagcgt cgatgacggc gaccatgtgc tcaccccatt atcttgactc cggtcccctt
     2701 gggcatctcc ttcaccagtt ccatgatggt gaggtccttg cccccggcgg cctcgatctt
     2761 ggccctagcg gactcggaga acccgtaggc ggccacggtg actttcttgc tcaactcccc
     2821 tgcccccaac accttccccg gtacgactat cacatcgccc tcggaggcgt atcgctccag
     2881 cttgctcagg ttgacctcag cccaattggc cttggccttc tccaatctta gagcaatgtc
     2941 tcgccagatc cccaccccgc tggccctgga ctccgccttc agggcggata tcacggccgc
     3001 aaggttgggg tcagtctttc cagctttcat gttctgactc tcccgacatt ggtaaccccc
     3061 tctaataagg aggggtatta aatacctttg ccatcgaagc gtgccatg
//
LOCUS       DAOSYN010000156         3090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_24854_length_3090_cov_3.635914, whole genome shotgun sequence.
ACCESSION   DAOSYN010000156 DAOSYN010000000
VERSION     DAOSYN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24854_length_3090_cov_3.635914"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLJ11_06210"
     CDS             <1..213
                     /locus_tag="PLJ11_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOO04298.1"
                     /translation="ALFLNNGGGAWDNAKKYIEAKGLKGTPQHAAGVVGDTVGDPFKD
                     TAGPSLHVLVKLLSTITLVFAVLFVV"
     gene            325..891
                     /locus_tag="PLJ11_06215"
     CDS             325..891
                     /locus_tag="PLJ11_06215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HOO04299.1"
                     /translation="MYLLAQREKVVRIPPHRLGEDLDTVVNELARESYEGRIEGNDSL
                     TVMVKNIEPVGEGRIVHGDGAVYQTVQFDTLLFRPMVNEVVEGRVCEVLKFGAFVRFG
                     PLDGLLHISQVMDDRMDADEANQRLIGKDSKRELKVGDQVRARIVTLSFNERNPRESK
                     IGLTMRQPGLGKKEWLAEDRAKKGGQEK"
     gene            888..1073
                     /gene="spt4"
                     /locus_tag="PLJ11_06220"
     CDS             888..1073
                     /gene="spt4"
                     /locus_tag="PLJ11_06220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HOO04300.1"
                     /translation="MKVTKACKVCSAITEEDKCPLCGGETSKDWQGYVVIIDHTKSEI
                     AKRMNIDKNGKFALKVR"
     gene            1114..1596
                     /locus_tag="PLJ11_06225"
     CDS             1114..1596
                     /locus_tag="PLJ11_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF359 domain-containing protein"
                     /protein_id="HOO04301.1"
                     /translation="MRAELSMPFGTMMQAEDLLRGIADCDRVIAVGDVVSSTLINKGV
                     IPHLTVYDRRNERRELEVGVHPADHLRMPETVVENPPGLITPGLVRAVKDGIEASDRN
                     KVRVEGEEDLAALVCAAIAPAGSCLVYGLPGKGAVLVRTSAEVNEAAQRLIRSMEVLK
                     "
     gene            1593..1901
                     /locus_tag="PLJ11_06230"
     CDS             1593..1901
                     /locus_tag="PLJ11_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04302.1"
                     /translation="MKIEIESKRPNALMDRTEVAFKVDHRGEGSPKRADVRTKLAEAL
                     AVSSGKVVIDHMETEFGMGVSTGYAKVYGSEESAKRFEKNYLQARNGLVEAKKAPKKK
                     "
     gene            1912..2103
                     /locus_tag="PLJ11_06235"
     CDS             1912..2103
                     /locus_tag="PLJ11_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HOO04303.1"
                     /translation="MAAKKKEVKKEAPSAKKDNYQIKGNKLERTRRSCPKCGPGVFMA
                     EHTDRTSCGKCGYTEFKKK"
     gene            2156..2229
                     /locus_tag="PLJ11_06240"
     tRNA            2156..2229
                     /locus_tag="PLJ11_06240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2191..2193,aa:Arg,seq:ccg)
     gene            2295..>3090
                     /locus_tag="PLJ11_06245"
     CDS             2295..>3090
                     /locus_tag="PLJ11_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HOO04304.1"
                     /translation="MQLTPEEQAMLAGKEGKAKRKAMGLLVALGTIYGADRLIPITSA
                     HLSGVSYKTIGEGGISFLEEMAEDGRVSVPTTLNPMGMDAERWREMGVDAHFAERQLH
                     ILSLYERMGVETNCTCTPYLLGNRPNVGDHVAWAESSALAFANSVLGARTNREGGPGA
                     LAAAIVGRTPNYGLHLDSNRRATHVVEVEQGIGKEEWSLVGHAVGRRLGSCIPYFRGL
                     GNDVNGLKALAAAMAASGSVAMFMVEGVTPEQPATLEGLPVVRISRQ"
BASE COUNT          652 a          915 c         1034 g          489 t
ORIGIN      
        1 gccctcttcc tcaataacgg aggaggggcc tgggacaacg ccaagaagta catcgaggcc
       61 aagggcctga agggcacccc gcagcatgct gcgggcgtgg tcggggacac cgtgggcgat
      121 ccgttcaagg ataccgccgg tccctcgctc cacgtgctgg tgaagctgct ctcgaccatc
      181 accctggtct tcgccgtgct cttcgtggtc tgagcgaaac cccttcccct tccttttttt
      241 atccagcatc ccgagcgctt atatgctcga tgctcaatct ttatataaca gatttatcat
      301 gccaaggtag ttaagggatt cctcatgtat ctgttggccc aaagggaaaa ggtggtccgc
      361 atcccccccc atcgcctagg ggaggacctg gacacagtgg tcaatgagct ggcccgcgag
      421 tcctatgaag ggcgcatcga gggcaacgac tcgctcacgg tcatggtcaa gaacatcgag
      481 ccggtgggcg aagggcgcat cgtgcacggg gacggggcgg tgtaccagac ggtccagttc
      541 gacactctcc tcttccgccc catggtcaac gaggtcgtgg agggccgggt gtgcgaggtg
      601 ctcaagttcg gggccttcgt acgcttcggg cctctggacg gcctcctgca cataagccag
      661 gtcatggacg accgcatgga cgcggacgag gccaaccagc gcctcatcgg gaaggactcc
      721 aagcgcgagc tcaaggtggg cgaccaggtc cgcgccagga tagtcaccct ctccttcaac
      781 gaacggaacc cccgggagag caagataggg ctgaccatga ggcagcccgg gctgggcaag
      841 aaggaatggc tggccgagga cagggccaag aagggggggc aggagaaatg aaggtgacca
      901 aggcgtgcaa ggtgtgctcc gccatcaccg aggaggacaa atgcccgctg tgcggggggg
      961 agacctccaa ggattggcag ggatacgtgg tcataatcga ccataccaaa tccgagatcg
     1021 ccaagaggat gaacattgac aagaacggga agttcgccct caaagtgcgc tgatctgccc
     1081 gtccctccca aggggtggag gatgcccgag cgcatgaggg ccgaactgag catgcccttc
     1141 gggaccatga tgcaggccga ggacctgctc aggggcatcg ccgattgcga cagggtcatc
     1201 gcggtggggg acgtggtctc cagcaccctt ataaacaagg gcgtcatccc ccatctcaca
     1261 gtgtacgacc ggcgtaacga gcgcagggaa ctggaggtgg gagtccatcc cgcagaccac
     1321 ctcaggatgc cggagacggt ggtggagaac ccccctgggc tgatcacccc cgggttggtg
     1381 agggcggtca aggacggcat cgaggcctcg gacaggaaca aggtccgggt ggagggggag
     1441 gaggacctgg ccgcgctggt atgcgccgcc atcgccccgg cagggtcctg cctcgtctac
     1501 ggcctcccag gcaaaggggc ggtgctggtg cgcaccagcg ccgaggtgaa cgaggcggcg
     1561 cagagactga tacgctcaat ggaggtattg aagtgaagat cgagatcgag agcaagaggc
     1621 ccaacgccct gatggaccgc accgaggtag ccttcaaggt cgaccaccgg ggggagggct
     1681 ccccgaagag ggccgacgtg aggaccaagc tcgcggaggc cctggccgtc agcagcggca
     1741 aggtcgtcat cgaccatatg gagaccgagt tcggcatggg cgtcagcacc gggtacgcca
     1801 aggtgtacgg cagcgaggaa tcggccaaga ggttcgagaa gaactacctc caggccagga
     1861 acggcctcgt tgaagctaag aaggcgccga agaagaagtg aggtgtgaac catggctgcg
     1921 aagaagaaag aggtcaagaa ggaagcgccc tcagccaaga aggacaacta tcagatcaag
     1981 gggaacaagt tggaacggac gaggaggagc tgtcccaaat gcggccccgg ggtgttcatg
     2041 gccgagcata ccgaccgcac ctcctgcggc aagtgcggct acaccgagtt caagaagaag
     2101 tgagccgctc ctcctcgaac gaggatgaag cggccttcaa gatcctaatg atcgcgggcc
     2161 tgtagtgtag cttggatatc acagaggcct ccggagcctc gaactggggt tcgaatcccc
     2221 acaggcccgc cttccccctc agcttatcta ttgcctccag taatttatta tcttccaatg
     2281 gcattgtcgg cggcatgcag ctgacccctg aagagcaggc catgctggcc ggcaaggaag
     2341 gcaaggccaa gcgcaaggcc atggggctcc tcgtggcgct tggaacgatc tacggcgccg
     2401 acaggctcat cccgatcacc tccgcccacc tctccggcgt gtcctacaag accatagggg
     2461 aggggggcat ctccttcttg gaggagatgg ccgaggacgg aagggtctcc gtgcccacca
     2521 ccctgaaccc gatggggatg gacgcggagc gctggaggga gatgggggtc gacgcgcact
     2581 tcgccgagcg ccagctgcac atcctgtccc tgtacgaaag gatgggcgtg gagacgaact
     2641 gcacctgcac gccctacctt ctgggcaatc ggccgaacgt cggagaccat gtcgcctggg
     2701 cggaatcgtc cgcgctcgcc ttcgccaact ctgtcctggg cgccaggacc aacagggagg
     2761 gcggcccggg ggccctggcc gccgccatcg tcggtcgcac ccccaactac ggcctccatc
     2821 tggacagcaa tcgccgcgcc acccacgtag tggaggtgga gcaggggata gggaaggagg
     2881 aatggtcgtt ggtcgggcat gccgtcggcc ggaggctcgg ctcctgcatc ccttacttcc
     2941 gaggtttggg caacgacgtc aacggcctca aggccctggc cgcggccatg gccgcgtccg
     3001 ggtcggtcgc gatgttcatg gtagagggcg tgacgcccga gcagcccgcg acattggagg
     3061 ggctgcccgt ggtgcgcatc tcccgccagg
//
LOCUS       DAOSYN010000157         3068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25069_length_3068_cov_4.643213, whole genome shotgun sequence.
ACCESSION   DAOSYN010000157 DAOSYN010000000
VERSION     DAOSYN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25069_length_3068_cov_4.643213"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLJ11_06250"
     CDS             <1..413
                     /locus_tag="PLJ11_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO04305.1"
                     /translation="VFNVDFSQANYLGLAVVVLVTTFAMTGFGLMLSSLGLFLRTSMI
                     VANIFLFVGLLVCGVNFPVAYLPAYLRPLSYAIPMTYGTEAARMAVGGAALGELSGPL
                     GAEALVGIAAMLVGYLMFRWFERLARSRGTLDRF"
     gene            455..1363
                     /locus_tag="PLJ11_06255"
     CDS             455..1363
                     /locus_tag="PLJ11_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOO04306.1"
                     /translation="MYGRVLERADVLLLQVLLRDSRASAAQIAEVTGLPAQAIPGMVA
                     RMREAGIIGDMTTKPSLLSLNARSVLVLGRSGLSSLQGLRMALERNENVAWMAIATGG
                     MAYVALHLREGADLEREVRRVASEAMMAQPKAMTRELFDHGRGGHAYTKDDMRILRAL
                     YRDSNKDLEQVAKETGVAVRKVRERFDLMAMKGALDFSVDFFPDNCDNLLAMLRLEVL
                     DPRGLEEKVGWLMGANAPYVMFFNAFPDTPGTLTSMVIPESMGGLRAVLRSFEESGAF
                     GHVATDLLLGSTIRDTWRDRALRGPE"
     gene            1576..2325
                     /locus_tag="PLJ11_06260"
     CDS             1576..2325
                     /locus_tag="PLJ11_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOO04307.1"
                     /translation="MKRVGWFTTGRGPGSLGLFNAVRSKVRGGELAAELSFVFLNRDV
                     RGNPYRAQLIAMAEEDGIPVLILPSDNFRSDLKAKDIDAWRAAYGEAMREMMAEHPMD
                     LGVLAGYMLILDPETCARYDIINLHPALPNTYQGTWEEIVRRVAESEDRTYGSMVHVC
                     TPELDRGRTVAYDEFGIEDLRERFHSKEELAKALRQRQVGREVPLLMETLKLLTDGDL
                     TLVRGELRDRMGRPLSIGPNLSARIDQLVHG"
     gene            2480..2812
                     /locus_tag="PLJ11_06265"
     CDS             2480..2812
                     /locus_tag="PLJ11_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04308.1"
                     /translation="MTSKVRVRMFPCDNVSTVEARSTDDGDFELKVSSTCPKVERFVS
                     TLSPLSLADLTDKASSKVFQEFMASDMSANCLTPSAVLTAAWVEAGMIARSNARKGIP
                     LMIEFVND"
     gene            complement(2840..2917)
                     /locus_tag="PLJ11_06270"
     tRNA            complement(2840..2917)
                     /locus_tag="PLJ11_06270"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2879..2881),aa:Glu,seq:ctc)
BASE COUNT          578 a          844 c         1057 g          589 t
ORIGIN      
        1 tcgtgttcaa cgtggacttc tcccaggcca actacctggg gctggcggtg gtcgtcctgg
       61 tcaccacctt cgccatgacc ggcttcggtc tgatgctcag ttccttgggc ctcttcctcc
      121 gcacctccat gatcgtcgcg aacatcttcc tgttcgtggg gctgctggtg tgcggcgtca
      181 actttccggt ggcctacctg ccggcatacc ttcggccttt atcctacgcc atcccgatga
      241 cctatgggac cgaggcggcg aggatggccg tggggggcgc cgccctcggg gagctgtccg
      301 gtccgttggg ggcggaggcc ctggtcggca tagcggcgat gctcgtcggc tacctgatgt
      361 tccgctggtt cgaacgcctg gccaggagcc ggggtaccct ggaccgcttc tgagcgggca
      421 catcattata tccgcgccgg cctgtagcga tcccatgtac ggcagggtgc tggagagggc
      481 ggacgttctg cttttgcagg tcctgctcag ggactcccgg gcttcggccg cccagattgc
      541 tgaggtcacc ggtcttcccg ctcaggccat cccagggatg gtggcccgga tgcgggaggc
      601 cgggatcatc ggggacatga ccaccaagcc cagcctgctc tccctgaacg caaggagcgt
      661 tctggtcctt ggaaggagcg ggctcagctc gctccaaggc ctccgtatgg cattggagcg
      721 gaacgagaac gtggcctgga tggccatcgc cacaggcggg atggcgtacg tggccttgca
      781 tttgagggag ggtgcggacc tggagaggga ggtccgccgg gtcgcctcgg aggcgatgat
      841 ggcgcagccg aaggccatga cacgggagct gttcgaccat gggaggggcg ggcatgcata
      901 caccaaggat gacatgcgca tccttagagc gctgtaccgg gacagcaaca aggacctgga
      961 acaggtcgcc aaggagacag gggtcgcggt caggaaggtc agggagcggt tcgatctgat
     1021 ggccatgaag ggcgcgctgg atttcagtgt ggattttttc ccggacaact gcgacaacct
     1081 tctggcgatg ctgcggctgg aggtgctcga ccctcgggga ttggaggaga aggtgggctg
     1141 gctgatgggg gccaacgccc cttacgtcat gttcttcaat gcgttccccg acacgcctgg
     1201 gacgctgacg agcatggtca tcccggagag catgggcggc ctcagggcgg tcctacgctc
     1261 ctttgaggag tccggggcct tcgggcatgt ggcgacggac ctgctcctcg ggtcgacgat
     1321 aagggatacc tggagggacc gggcgctgcg cggtcctgaa taagcgtgag ggcgggcaat
     1381 gacagtgagc gatgggctgc ggtccgatgg acagcatcga tcagggcttg gacgtcgacc
     1441 ctctctccgg cattcgaagc tttggggctg gaattgcatg agggccagca ggggacgccg
     1501 gggatgcttg tccctcctta ttccctggac cgaccgatgc gattaaaata ggtcgggagc
     1561 ggatatggga tgggaatgaa gcgtgtaggt tggttcacca ctggcagggg accggggtca
     1621 ctggggctct tcaacgccgt ccggtccaag gtccgagggg gcgagcttgc ggccgagctg
     1681 tccttcgtct tcctgaacag ggacgtcagg ggcaacccct atcgcgccca gctcatcgcg
     1741 atggccgagg aggacggaat accggtcctt atcctcccat cggacaactt ccggagcgat
     1801 ctgaaggcga aggacatcga cgcctggcgc gccgcctatg gggaggcgat gcgggagatg
     1861 atggccgagc atcccatgga cctgggggtg ctggccggct acatgctcat ccttgatccg
     1921 gagacctgcg cccggtacga catcataaac ctgcacccgg ccctcccgaa cacctaccag
     1981 ggcacatggg aggagatcgt gcgccgggtg gccgagagcg aggataggac atatggctcg
     2041 atggtgcacg tgtgcacccc cgagctggac agggggagga ccgtggccta tgacgagttc
     2101 ggcatcgagg acctccggga acgcttccat tcaaaggagg agctagccaa ggccctgcgc
     2161 cagagacagg tgggccgcga ggtgcccctg ctgatggaga cgctcaagct gctgaccgat
     2221 ggggacctca cgctggtccg gggggagctc agggaccgga tgggcaggcc attgagcatt
     2281 ggcccgaacc tttccgccag gatcgatcag ctcgtacatg gatgacccgg gagggatgaa
     2341 gagggaccga tggaccgaag atcgggggga ccgtcctaaa gaagtctgaa accgatcggg
     2401 cctgtaggat tttcgaatat tcttgagaca tcttaaatcg aggggcggtt gttacagtga
     2461 taaaacggag agggttgaga tgacatcgaa ggtcagggtc aggatgttcc cttgcgacaa
     2521 cgtatcgacc gtcgaggcgc ggagtacgga cgatggggat tttgagctga aggtcagcag
     2581 cacctgtccc aaggtggagc ggttcgtgag caccctctcc ccgttgtcct tggccgacct
     2641 caccgacaag gcgagcagca aggtcttcca ggagttcatg gcctcggaca tgagcgctaa
     2701 ctgcctcacg ccctcggcgg tgctgacggc cgcttgggtg gaggcgggga tgatcgctcg
     2761 gtccaacgct aggaagggca ttccgttaat gatcgagttc gtgaacgatt gactagtgat
     2821 gcttgatgaa gaagaaaggt ggtgctcccg tcgggatttg aacccgagtc gaagcctcga
     2881 gagggcttca tgattggccg gactacacta cgggagcgtg taggtcccca attggatggc
     2941 tgtaattaaa tcttacggat ggcaagaggc ggaagggata tatccataca atcccacgta
     3001 cacctttgaa tgcggttcga gcacgatcac cgttccctca cgatcaattt ggcgttgtat
     3061 gccatcgt
//
LOCUS       DAOSYN010000158         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25082_length_3067_cov_4.476096, whole genome shotgun sequence.
ACCESSION   DAOSYN010000158 DAOSYN010000000
VERSION     DAOSYN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25082_length_3067_cov_4.476096"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLJ11_06275"
     CDS             complement(<1..866)
                     /locus_tag="PLJ11_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO04309.1"
                     /translation="MVFDYANRLKLLPPYLFAEIEEKVERKRKEGVDIIDFGIGDPDL
                     PTPRPIVEFIKRELDDPGNHRYPSSAGEADTRKAIAQWYKERFKVDVDPNGEVAILIG
                     SKEGLANICRAFVNPGEKVLAPDPAYPVYAQGGALLTDAVPVRFPLLPERGFLPDMES
                     LPTDARMVYLNYPNNPTGAVCDEEFLLGASRWCIETDTILCYDNAYSEMTFDDYIAPS
                     ALQVAQGDGTIEFGSMSKTFNMTGYRIGYAVGDADLISGLKKVKSQIDSGAPKFLQRA
                     VIMALGAYEGGE"
     gene            complement(873..1697)
                     /gene="dapF"
                     /locus_tag="PLJ11_06280"
     CDS             complement(873..1697)
                     /gene="dapF"
                     /locus_tag="PLJ11_06280"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOO04310.1"
                     /translation="MRFWKYHGLGNDFVLVEDLDGTAAKDPDLVRSVCHRRFGVGADG
                     VLYLGRSERADFAMRVMNADGSEAEMCGNGIRCLAKHIYDMGYKREERLTIETLAGVK
                     EVTVAVVDGKVATATVDMGSPVLDRPAIPMRGEGRFINQGIEVAGKVVHGTAVSMGNP
                     HLVIFDPLPDEDIERLAPLLHAHELFPRRTNVEFVKWRDGILEARVYERGAGWTMACG
                     TGACATAVAANLLGTIELGREVQVRLPGGSLWITVAQDLSRVTMRGPATRVFEGRM"
     gene            complement(1694..2950)
                     /gene="lysA"
                     /locus_tag="PLJ11_06285"
     CDS             complement(1694..2950)
                     /gene="lysA"
                     /locus_tag="PLJ11_06285"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOO04311.1"
                     /translation="MREFQRRKDTMLIGGVSATKIAARFGTPVMVTDEDALRENYRAL
                     HRAFNAVFPTRVHYACKANTNLSILRILEQEGSCIDAVSIGEVDSCLRAGFSPDRILY
                     TGVGVSEEEMRQVVERKVPINVDSIGQLRRLAAIDSHHPIALRVNPDVGAGHSQKVVT
                     GTKGAKFGIPKEEIVQAFAEALRLGFSPKGLHAHTGSGGSDPTVFGRVTEVLVDIFER
                     VRSELGLDLEFLDIGGGISIPYRPEELRPDLDAIAAEVAGKLKGSSVRTLVVEPGRYI
                     VADTTVLLTRVNDVKSTPVKDYVLVDAGFNTLIRPAFYDAYHHVAVANKFGQQAQLKY
                     DVAGPLCESGDYLAKERYLPKVEVGDLLCVYDVGAYGFSMSSNYNTRPRCAEVLVWEG
                     KPFLIREREGLKDLLRHQKVPPRLSQ"
BASE COUNT          496 a         1053 c          924 g          594 t
ORIGIN      
        1 ctctctcctc cctcatacgc ccccagggcc atgatgaccg ccctctgcag gaacttgggc
       61 gcgccggaat cgatctggga cttgaccttc ttcaggccgg agatgaggtc ggcgtccccc
      121 accgcgtagc cgatgcggta gccggtcatg ttgaatgtct tcgacatgga gccgaactcg
      181 atggtcccgt ccccctgggc cacctgcagc gcggagggcg cgatgtaatc gtcgaaggtc
      241 atctccgagt aggcgttgtc gtagcacagg atcgtatcgg tctcgatgca ccaccttgag
      301 gcgcccagga ggaactcctc gtcgcacacc gccccggtgg gattgttggg gtagttcagg
      361 tagaccatgc gggcgtccgt cggcagggac tccatgtccg gcaggaagcc ccgttccggt
      421 agaagcggga agcgcaccgg gacggcgtcc gtcaggaggg cgcccccttg ggcgtagacg
      481 gggtaggccg ggtcaggggc caggaccttc tcccccgggt tgacgaaggc ccggcagatg
      541 ttcgccagcc cctccttgga gccgatgagg atggccacct ccccgttggg gtccacatcc
      601 accttgaagc gctccttgta ccactgggcg atggccttcc gggtgtcggc ctccccggcg
      661 ctggaggggt agcggtggtt ccccgggtcg tccagctccc tcttgatgaa ctcgacgatc
      721 ggccttgggg tcggcaggtc cgggtccccg atgccgaaat cgatgatgtc caccccttcc
      781 ttcctcttcc tttccacctt ctcctcgatc tccgcgaaga gatagggagg cagcaacttc
      841 agcctgttag cgtaatcgaa caccatagcg tctcacatcc gcccttcgaa cacccgggtc
      901 gccgggccgc gcatggtcac ccgcgagagg tcctgtgcta cggttatcca caggcttccg
      961 cccgggagcc gcacctgcac ctccctgccc agctcgatgg tccccagcag gttggctgcc
     1021 accgcggtgg cgcaggcgcc agtcccacag gccatggtcc atccggcccc tctttcgtac
     1081 acgcgcgcct ccagtatccc atccctccat ttaacgaatt ctacgttggt acggcgaggg
     1141 aaaagctcgt gggcgtggag gaggggggcg agcctttcga tgtcctcgtc cgggagcggg
     1201 tcgaagatca ccagatgagg gttgcccatg gacaccgcgg taccatgcac caccttcccc
     1261 gccacctcga tcccctggtt gatgaacctt ccctcccccc tcatcgggat ggcgggacgg
     1321 tccagaacgg ggctgcccat gtccaccgtg gccgtcgcca ccttgccgtc gaccacggcc
     1381 acggtgacct ccttcacgcc ggccagggtc tctatcgtca gcctctcctc ccgcttgtac
     1441 cccatatcgt agatgtgctt ggccaggcac cggatgccat tgccgcacat ctccgcctcc
     1501 gagccgtcgg cgttcatcac ccgcatggcg aagtcggccc tctcgctcct ccccagatag
     1561 agcacgccgt ccgccccgac gccgaaacgc cgatggcaca ccgagcgcac gaggtccggg
     1621 tccttggccg ccgttccatc aaggtcctcc acaagcacga agtcgttgcc cagcccgtga
     1681 tatttccaga acctcattgg ctcagcctcg ggggcacctt ctggtgacga aggaggtcct
     1741 tgagcccttc cctctccctg atcaggaagg gtttcccttc ccacaccagg acctccgcgc
     1801 agcgcggcct ggtgttgtag ttggaggaca tcgagaagcc gtaggccccg acgtcgtaga
     1861 cgcacaggag gtcgcccact tccaccttgg gcaggtaacg ttccttcgcc aggtagtccc
     1921 cgctctcgca gagggggccg gccacatcgt acttgagctg ggcctgctgc ccgaatttgt
     1981 tggccaccgc cacgtggtga taggcatcgt agaacgcagg gcggatcagc gtgttgaagc
     2041 cggcgtccac taggacgtag tccttcaccg gggtggactt gacatcgttc acccttgtca
     2101 gcaggacggt ggtgtccgcg acgatgtacc ttccaggctc cacgacgagc gtcctgacgc
     2161 tgctgccctt cagcttgccg gcgacctccg cggcgatcgc gtccaggtcc ggcctcagct
     2221 cctccgggcg gtaggggatg ctgatgcccc cgcctatgtc caggaactca aggtccaggc
     2281 ccagctcgct ccgcacgcgt tcgaatatat ccaccaggac ctcggtgacc ctgccgaaaa
     2341 cggtgggatc gctccccccg gagccggtgt gggcgtgcag gcccttcggg gagaagccga
     2401 gcctgagggc ctcggcgaag gcctggacga tctcttcctt ggggatcccg aacttggccc
     2461 ccttggtccc ggtgaccacc ttttgggagt gcccggcacc gacgtcgggg ttcaccctca
     2521 atgctatcgg atgatggctg tcgatggccg ccaggcgcct gagctgccct atggagtcca
     2581 cgttgatggg caccttgcgc tccaccacct gccgcatctc ctcctccgag accccgaccc
     2641 cggtgtacag gatccggtcg ggggagaagc ccgcccgaag gcacgagtcc acctcgccga
     2701 tggatacggc gtctatgcag gagccctcct gctcaagtat gcgcaggatg ctgaggttcg
     2761 tgttggcctt gcaagcgtaa tggacgcgcg ttggaaagac ggcgttgaac gccctgtgca
     2821 gggcgcggta gttctccctt agggcgtcct cgtcggtgac catgaccggg gtgccgaagc
     2881 gcgcagcgat cttggttgcc gacaccccgc cgatgagcat ggtgtccttt ctcctctgga
     2941 actctctcat tcaagcacct acttggatat gtacagctcg tgcagccttc tcacgacctc
     3001 gatggccttg gagcccccga ccatgaagtt gagcgctatg tcagaggctc cctccgatat
     3061 aagctcc
//
LOCUS       DAOSYN010000159         3044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25313_length_3044_cov_3.183004, whole genome shotgun sequence.
ACCESSION   DAOSYN010000159 DAOSYN010000000
VERSION     DAOSYN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25313_length_3044_cov_3.183004"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PLJ11_06290"
     CDS             <1..1380
                     /locus_tag="PLJ11_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOO04312.1"
                     /translation="LYYDEDPRPWLFPILLCFLIGLMLLLRYRSPEVTRPTEAMFIVA
                     TAWLVMAIMGAIPFVMYGMGVVDALFEAMSGFTTTGSSIMSDIESWPKSILFWRSFTQ
                     WLGGAGVIMVFVTVLPMLGVGGRNLFKNEFPGLDVQNFSARIQEEARKFHYIYGFFSL
                     AMLVLLLLTGIGVYDSLCVMFSTVSTGGLSPHSESIAYYASASVEWIVIIFMFLAATN
                     FYLHFQAITTRDPYRFFRSSEFRLYLGIVLAATAAITAFLWGKEFSDLGDGVRTSMFH
                     VVSLMTSTGFATANYIAWDQAVVFLLLALIVIGGSTGSTAGGIKVARFFLTYKFIASA
                     LHKTVHPRSIFTVKLDGRPLSEEALSSVVAMVMCYFATALVAVVTLILAGVDPVTSVS
                     AAVTSLSNCGPGLGMLGPYGSFGPLPVIAKLILTFTMWAGRLEFISVLVLFTPVFWRE
                     LMRYRERYV"
     assembly_gap    1781..1790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1811..2518
                     /locus_tag="PLJ11_06295"
     CDS             1811..2518
                     /locus_tag="PLJ11_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO04313.1"
                     /translation="MPKVKRKVIRIDEGRCTGCGECVLACAEGAIVIRDGKAKVVKDM
                     YCDGLGACLGHCPEGALAIEEREAEPFDEGAAKEHAASGREGEQACSGSRPMAITPLA
                     PASGEERASQLRNWPIKLALAPEVSPVYRDATLLLAADCTGFSMGNVQRDFIAGRIAI
                     IGCPKLDDFQAQVDKLTRILKGNAIRDVTLVRMDVPCCGGLQRMVREAMRLSGKRMPL
                     REYIVERYGGKVIEVTA"
     gene            complement(2508..>3044)
                     /locus_tag="PLJ11_06300"
     CDS             complement(2508..>3044)
                     /locus_tag="PLJ11_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOO04314.1"
                     /translation="EAVPIAGARTMVGSSAPIVAEGYGDEMRRAEREIRDPGELEDVI
                     RRAEVCRLGMVDGGEPYIVPMNFGYRDGHLYFHCAREGRKLDVLRANPRVCFELEAGV
                     RTVRGETACQWSTAYESIIGWGTAMVLLDEEGVRKGLEVLMEHYFEGLYAFDPRSLSL
                     TAIIRVDVERMTGKRTMR"
BASE COUNT          521 a         1022 c          926 g          565 t
ORIGIN      
        1 ctctactatg atgaggaccc ccgcccctgg ctcttcccca tcctgctctg cttcctgatc
       61 ggcctgatgc tcctgctgcg ctaccgttcg ccggaggtca ccaggccaac ggaggcgatg
      121 ttcatcgtag ccactgcctg gttggtgatg gcgatcatgg gggcgatccc cttcgtcatg
      181 tacgggatgg gggtggtgga cgccctgttc gaggcgatga gcggctttac gaccaccggt
      241 tcaagcatca tgagcgacat cgagtcctgg ccgaagagca tcctgttctg gcgcagcttc
      301 acccagtggc ttggaggggc gggcgtgatc atggtcttcg tcaccgtcct cccaatgctg
      361 ggcgtgggcg gccgcaacct cttcaagaac gagttcccgg gcctggacgt acagaacttc
      421 tccgcgcgca tccaagagga ggcgcgcaag ttccactaca tatacggctt cttctcgctc
      481 gccatgctgg tgcttctcct cttgacgggc atcggggtgt acgactccct ttgcgtgatg
      541 ttctccacgg tctccacggg gggcctgtcg ccgcactcgg agagcatagc ctactatgca
      601 tcggccagcg tggaatggat agtcataatc ttcatgttcc tcgcggccac caacttctac
      661 ctgcacttcc aggccataac gaccagggac ccctaccgct tcttccgaag ctcggagttc
      721 cggctgtacc tgggcatcgt cctggcggcc acggcggcca tcaccgcctt cctgtggggc
      781 aaggagttca gcgatctggg ggacggggta aggacctcca tgttccatgt cgtgagcctg
      841 atgacctcca ccggtttcgc gaccgcgaac tacatcgcct gggaccaggc cgtggtgttc
      901 cttctgctcg ccctcatcgt catcgggggc agcactggct ccaccgcggg gggcatcaag
      961 gttgccaggt tcttccttac ctacaagttc atagcctccg cgctgcacaa gacggtccat
     1021 ccccgctcga tattcacagt gaagctggac ggcaggccgt tgagcgagga ggcgctctcc
     1081 tcggtggtgg ccatggtcat gtgctacttc gccaccgccc tggtggccgt ggtcaccctc
     1141 atcttggcgg gggtggaccc tgtgacctcg gtcagcgcag ccgtgaccag cctctccaat
     1201 tgcggcccag gcctgggcat gcttggccca tacggctcct tcgggccgct ccccgtcatc
     1261 gcaaagctca ttctcacgtt caccatgtgg gccggtcgtc tggagttcat ctcggtcctg
     1321 gtgctgttca ccccggtgtt ctggcgggaa ctcatgagat accgggagag gtatgtatga
     1381 cattcgtgca catccctccc gaatggacca tcccatcccc cgcccatgcc atggaggaga
     1441 tcgccctcca atttccgtag acctgacctg aaagggattg ggacgcgttc cgaggccata
     1501 ggatggggcg atggtccccc gcaccgccag cccggcagcg gcccccgcgg cggccgcacc
     1561 ggcccgcacc accgcgatca acggcgtccc agaagcgctc gcccccagcg catttcgcct
     1621 ccttgccggg gttccctgtg ccgtgggtgg acaggacggc cccgaaggaa gtcgaaggcg
     1681 cggccgccag aggaggacgg tatcgcatct tcgtgagata gaccatcgtt ctcgaaccgg
     1741 gaaagggggt ggagcgggga ggcaaagccc ttaaggggga nnnnnnnnnn agtcccatgg
     1801 aacgcctgcg atgcccaagg tcaagcggaa ggtgatcagg atagacgagg ggcgctgcac
     1861 cggatgcgga gaatgcgtat tggcctgcgc cgagggtgcc atcgtcatca gggacgggaa
     1921 ggccaaggtc gtcaaggaca tgtactgcga cggcctcggg gcctgcctgg ggcactgtcc
     1981 ggagggcgcc cttgccatcg aggagaggga ggccgaaccg ttcgacgaag gggccgccaa
     2041 ggagcacgcc gcctcgggaa gggagggaga gcaggcctgc agcggctccc gtcccatggc
     2101 gatcacgccc ctcgctcctg cgtccgggga ggagagggca tcccagctca ggaactggcc
     2161 tatcaagctt gccctggccc ccgaggtctc cccggtctac agggacgcga cgctgctgct
     2221 cgccgccgac tgcaccggct tctccatggg caatgtccag agggacttca tcgccggccg
     2281 catcgccatc atcgggtgcc ccaaactgga cgatttccag gcccaagtgg acaaactgac
     2341 caggatcctc aagggcaacg ccatcaggga cgtcacgctg gtccgcatgg acgtaccctg
     2401 ctgcgggggg ctgcagcgca tggtccggga ggccatgcgg ctcagcggca agcggatgcc
     2461 cctccgcgag tatatcgtcg agcgctacgg cggcaaggtg atcgaggtca ccgcatagtc
     2521 cgcttgccgg tcatccgttc cacgtccacc cgtatgatgg cggtcaacga gagcgagcgg
     2581 gggtcgaagg cgtacaggcc ttcgaaataa tgttccatca ggacctccag ccccttcctc
     2641 accccttcct cgtccagaag gaccatggcc gtcccccagc cgatgatgct ctcgtaggca
     2701 gtggaccact ggcacgccgt ctccccccgc accgttctca ctcccgcctc cagctcgaag
     2761 catacccggg ggttggcccg caggacatcc agcttgcgac cctcccgggc gcagtggaag
     2821 tacaggtgtc cgtcgcgata cccgaagttc atgggcacga tgtacggctc cccgccatcc
     2881 accatcccca gccggcagac ctcggcccgc ctgatgacgt cctccagctc cccggggtcc
     2941 ctgatctccc tctccgctct cctcatctca tcaccgtatc cttccgccac aataggggca
     3001 gaactcccca ccatggtccg agcgcccgca atagggacag cttc
//
LOCUS       DAOSYN010000160         3038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25373_length_3038_cov_2.948709, whole genome shotgun sequence.
ACCESSION   DAOSYN010000160 DAOSYN010000000
VERSION     DAOSYN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25373_length_3038_cov_2.948709"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..988)
                     /locus_tag="PLJ11_06305"
     CDS             complement(86..988)
                     /locus_tag="PLJ11_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOO04315.1"
                     /translation="MRLYRVGKVKKVFEVDDRTLEFHFTNNISVFDKIIPTEIPHKGE
                     VLCRTSAYWFKLLEENGIRSHFLRLTGKDRMLVRRVNVIPDYSKLNSATTNYLIPLEF
                     ICRHYNAGSLNDRVREGKVDVERLGFSKGHQLRYGERLPRPFFEATTKLEETDRNLTD
                     EEAMRISGLTEGEYREVFDTIARIDALIAGRAEGAGLIHVDGKKEFAFDEGRRLMIVD
                     TFGTLDEDRWWDAAEYAKGNCVELSKEMVRQYYREIGYHAALYKARMEHAKEPPIPPL
                     PEEMAQRVSRLYIDMYERMTGERF"
     gene            complement(1036..1728)
                     /locus_tag="PLJ11_06310"
     CDS             complement(1036..1728)
                     /locus_tag="PLJ11_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KaiC domain-containing protein"
                     /protein_id="HOO04316.1"
                     /translation="MERVRTGIDGLDEMLYGGIPKGSTVVVMGSFGTGKTTLGLQFLN
                     QGLAEGEKGVFITLEEDRDSILADAEAHGWDLRGKMDAKQLEIVKLEPNDAKVTIQRI
                     KSELPEFIKKFGASRIVVDSISLLNLLFDTDHDRRANLFNLSQMIKRTGATLLMTAEV
                     KDDNPFASRDGLVEYVADGVISLRYLELLERNELMLSMRIIKMRRTEHSRKITPYSIS
                     GKGIEVHVGSEF"
     gene            complement(1734..2564)
                     /locus_tag="PLJ11_06315"
     CDS             complement(1734..2564)
                     /locus_tag="PLJ11_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04317.1"
                     /translation="MKRIPSGVSDFDSIIKGGIPVGSVILLMGDLGAGQQEYALTSAA
                     KISLVKENPAVADYYLGHRVGQRVLPKKICYVTFARSQEDILQEIRTSFNEDFYGAFE
                     RNVTFKDFSKQYFRNTLVPRSWTGDTSVGLFSDDGEQNTLEALVDYLDANAHDSMVII
                     DSLTDLVVNNRIEEMDLVAVMRGMQRMAKRWKGVIYLLLTKDIVDARKEKLIMDSVDG
                     ALVFEWSRFAQSSRRQRYMYVEKFMSILPHLDQQRIARFATVVTAQQGLVVIDTERIG
                     "
BASE COUNT          528 a          962 c          891 g          657 t
ORIGIN      
        1 tgatgctggg cgtcaccctg ctgtcgctga tgatctggat aatcgacatg tcggtgtacg
       61 gagggtgatg ggcagggagg atggctcaga acctctcccc ggtcatccgc tcgtacatgt
      121 caatgtagag cctgctcacc ctctgcgcca tctcctccgg cagcggcggg atgggcggtt
      181 ccttcgcgtg ctccatgcgg gccttgtaga gcgcggcgtg atagccgatc tcacggtaat
      241 actgtcggac catctccttg gagagctcga cgcagttgcc cttggcgtac tcggcggcgt
      301 cccaccagcg gtcctcgtcc agcgtgccga atgtgtcgac gatcatcagg cgacggccct
      361 cgtcgaacgc gaactccttc ttcccgtcca cgtggatcag gcccgcccct tcggcccgcc
      421 cggcgatcag cgcgtctatc ctggcgatgg tgtcgaagac ctccctgtac tccccttccg
      481 taaggccgga gatgcgcatg gcctcctcgt ccgtcagatt gcggtccgtc tcctccagct
      541 tcgtcgtcgc ctcgaagaac ggcctcggca gcctctcgcc gtacctcagc tgatgcccct
      601 tcgagaagcc cagccgttcc acgtcgacct tcccttccct gacccggtcg ttcaaagaac
      661 cggcgttgta gtgacggcat atgaactcca gagggatgag gtagttggtc gtcgcgctgt
      721 tcagcttgga gtagtcaggg atgacgttga cgcgcctcac cagcatgcgg tccttgccgg
      781 tgagccggag gaagtgggag cggatgccgt tctcctccag gagcttgaac cagtaggcgg
      841 aggtgcggca gagcacctcg cccttgtgcg ggatttccgt gggaatgatc ttgtcgaaca
      901 ccgagatgtt gttggtgaaa tggaactcga gcgttcggtc gtcgacctcg aagaccttct
      961 tgaccttgcc cacccggtag agcctcatcg ccgcagggat ggcggaggca cgattaaagc
     1021 ttgccttttc ctccctcaga actccgagcc cacgtgcacc tctatgccct tccccgatat
     1081 ggaatagggg gtgatcttgc gggaatgctc cgtcctgcgc atcttgatta tgcgcatcga
     1141 cagcatgagc tcgttccttt ccagcagctc gaggtagcgg agcgagatca ccccgtcggc
     1201 cacgtactcg accagcccgt cccgggaggc gaaggggttg tcgtccttga cctcggcggt
     1261 catcagcagc gtcgcgccgg tcctcttgat catctgggag aggttgaaga ggttcgcgcg
     1321 ccggtcgtga tcggtgtcga acagcagatt cagcagcgag atggaatcga cgacgatgcg
     1381 ggacgccccg aacttcttga tgaactcggg cagctcgctc ttgatccgct ggatggtgac
     1441 cttcgcgtcg ttgggctcga gcttgactat ctccagctgc ttggcgtcca tcttgccgcg
     1501 caggtcccag ccgtgcgcct cggcgtccgc gaggatggag tccctatcct cctccagggt
     1561 gatgaatacg cccttctccc cctccgccag cccctggttg aggaactgca ggccgagggt
     1621 ggtcttgccc gttccgaagg agcccatcac aaccacggtc gagcccttcg ggatcccccc
     1681 gtagagcatc tcgtcaagcc cgtctatccc tgtcctgacc ctctccattc ccatcaccct
     1741 atcctttccg tgtcgatgac caccaggccc tgctgcgccg tgaccacggt ggcgaacctt
     1801 gcgatgcgct gctggtccag gtgcggcagg atgctcatga acttctccac gtacatgtag
     1861 cgctgccgcc gggacgactg ggcgaacctg ctccattcga agaccagcgc cccgtccacc
     1921 gagtccatga tcagcttctc cttccttgcg tccactatgt ccttggtcag caggaggtag
     1981 atcacgccct tccaccgctt ggccatgcgc tgcatgccgc gcatcaccgc caccagatcc
     2041 atctcctcga tgcggttgtt gaccacgagg tccgtcagcg agtcgatgat gaccatgctg
     2101 tcgtgcgcgt tggcgtccag atagtccacc agggcctcca gggtgttctg ctccccgtcg
     2161 tcggagaaca gcccaaccga ggtgtccccg gtccagctcc tcgggaccag ggtgttgcgg
     2221 aagtactgct tggagaagtc cttgaacgtc acgttccgct cgaacgcccc gtagaagtcc
     2281 tcgttgaagg acgtccttat ctcctgcagg atgtcctcct gggagcgggc gaaggtgacg
     2341 tagcagatct tcttgggcag gaccctctgc ccgaccctgt gccctaggta gtagtcggcc
     2401 acggcggggt tctccttgac cagggagatc ttggcggcgg aggtcagggc gtactcctgc
     2461 tgaccggccc ccaggtcgcc catgagcagg atgaccgacc cgacggggat cccgcccttg
     2521 ataatgctgt caaaatcgga gacaccgctg ggtatcctct tcaccgtcgc atccattcct
     2581 gcaccgaacc cggcatacaa tgggatggga cgatatcaag tttatgtgct catccgcttc
     2641 aatggagcct gggccgttaa accgtgctga cggtttcgga cattgagggt ggagggggag
     2701 agcgaacatg ctgagctaga tccacttctg cccgctcatc gttcgtgccc gaaaagaacg
     2761 cctggggtca ttttaaggtt cttttctgcc tatgacccgg taaccgccgt tcgttctctt
     2821 acacaagagc aggtctcagt aacatctgtc acattgaaag gggggcttat ggccatgggg
     2881 gctttatgat gcaggacctc ctccagcgcg gtgagctgca tagaggtcat gcccaaggtc
     2941 cgatgcgcca ttggaaaaag ggaacggata tgcatgcgaa ctggggctca gccccactcc
     3001 acccaagcat cgggcgtgca tttcccgtct atatgcga
//
LOCUS       DAOSYN010000161         3026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25498_length_3026_cov_3.759004, whole genome shotgun sequence.
ACCESSION   DAOSYN010000161 DAOSYN010000000
VERSION     DAOSYN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25498_length_3026_cov_3.759004"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..388)
                     /locus_tag="PLJ11_06320"
     CDS             complement(47..388)
                     /locus_tag="PLJ11_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04318.1"
                     /translation="MVEEGELPLQARVRVDPGVCRLPSVIQARSDGTTVEFEVVNSDC
                     PQVRNLTKVLRRMEIWDVMRMPYVDNAVYQICGEVLKHSACPVPMAMIKCAEAAAELG
                     LKRSVVVEFVP"
     gene            540..2369
                     /locus_tag="PLJ11_06325"
     CDS             540..2369
                     /locus_tag="PLJ11_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOO04319.1"
                     /translation="MQTTKLSDAAFPQGPELDTAEARALLWMSDPQLHEALQASKDIE
                     AARHNIMAILHERESRLHARDCQIHALERSNALHCLRVMRNFLSPRNERVSGTSVVHH
                     LLCLEQDDGCGDVHPAFVHDILHIMKGTTGAPVLALQEAPDLTVMDEREGSRRRSDYL
                     DGLAKAVEAKASRYVSGLEPSVIERRRKNRERILDHLNATLDDWNDYPWQMRNVLLSE
                     EVIGDLIELTDSEREAIRLCGENRIPFGITPYYLSLMDREPSRRLDHAVRAQVIPPLN
                     YIHGILKARADPSATLDFMREGQTSPVDLVTRRYPMIAIFKPYNTCAQICVYCQRNWE
                     IEGVLSPDAMAPPEKIEAALDWFRDHPMVSEVLITGGDPGVLSNGHLRRLLGRLMELP
                     HVRRLRLGTRVPVVMPMRIDPGFLDLMAAVHDPPGREFALVTHFEHPYEVTPEAAEAV
                     RSLRTLGISVYNQQVFTMENCRRFETVAVRSTLKRIGVDPYYTFNAKGKEETSEYRVP
                     ISRLLQERKEEARLLPGLSRTDEPVFNIPALGKNHLRAWQHHDLIAITPRGERVYEFH
                     PWEKNIAVAPTYVHMDVPILDFLIALAERGEDVREYESIWYYF"
     gene            complement(2366..>3026)
                     /locus_tag="PLJ11_06330"
     CDS             complement(2366..>3026)
                     /locus_tag="PLJ11_06330"
                     /inference="COORDINATES: protein motif:HMM:NF021410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HOO04320.1"
                     /translation="NCAVLLKGAADRVRLGCEQRDEAVRAGAVGATTLVVDQGRIVFP
                     GDEEFPDQSLVAPLKNYFRMDDGDVIIIGSAFSYDAAEKGAVTAALALSNLSRRCWNE
                     GTELLSRDTEADELRCLALAVHELLGRLPVTMRSRNRPGVRCEDGEVIDTHYTGPMLE
                     EAMKRGAIVRRVSTSGPYRGLPVIAVPIMRKREAIAAIATMDIGKGSLSELLAKLAER
                     Y"
BASE COUNT          609 a          972 c          907 g          538 t
ORIGIN      
        1 cgccgtcgtg ggtatgccct ccatgaacga gctgctgacc aagtgatcag ggaacgaatt
       61 cgactaccac cgacctcttg aggccgagtt cggcggcggc ctcagcgcac tttatcatgg
      121 ccataggcac tgggcaggcg gaatgcttga ggacctcgcc gcatatttga tagacggcat
      181 tgtccacgta cggcatcctc atcacatccc agatctccat tcggcgcagg accttggtca
      241 gattgcggac ctgagggcaa tcgctattga cgacctcgaa ctccacggtc gtcccgtcgc
      301 tccgagcctg gatgacacta ggcagccggc acaccccggg gtccaccctg acccttgctt
      361 gcaggggaag ctccccttct tcgaccatcc cccctcgatc gcgctcaagt cagataatca
      421 tatcctttgg aatgcgctcg gaatgggcaa ataataagta agatgggttc ctgatgaata
      481 gcgtcgctcc tcgggacgat ttccttttcg gttcggtatg ggcggcaaag gtgagaccta
      541 tgcaaaccac gaagctatca gacgcagctt tcccccaggg accggaactc gataccgccg
      601 aggcccgcgc cctgctctgg atgagcgacc ctcaattgca tgaggccctt caagcctcca
      661 aggacatcga ggccgctcgc cacaacataa tggccattct ccatgaaagg gaaagcaggc
      721 tgcacgcccg ggactgccag atacacgccc tggaaaggtc gaacgcgctc cattgcctcc
      781 gcgtgatgcg caactttctc tcccccagga acgaaagggt gagcggcact tcggtggttc
      841 accacctcct ctgcctcgag caggatgatg gatgtggcga tgttcacccc gccttcgtcc
      901 acgatatatt gcacatcatg aagggcacca caggggcacc agtgctagcg cttcaggagg
      961 cccccgatct cactgttatg gacgaaaggg aaggaagccg gcggcgctca gactacctcg
     1021 acggcctggc caaggcggtg gaggcaaagg catctcgtta cgtcagcggc ctcgagccca
     1081 gcgtcataga gcggcgcagg aagaaccgcg agcggatcct ggaccacctg aacgccacat
     1141 tggacgattg gaacgactat ccatggcaga tgaggaacgt ccttttgagc gaggaggtaa
     1201 taggggacct catcgagctg acggactcgg agcgcgaggc catccgcctc tgcggggaga
     1261 accgcatccc gttcggaata accccttact acctctccct catggatagg gagccttccc
     1321 ggcgcctcga ccatgcggtg cgggcgcagg tcatcccacc gctcaactac atccacggga
     1381 tactcaaggc cagggcggat ccctccgcca ccctggactt catgcgggag gggcagacct
     1441 cccccgtgga cctggtcacg cggcggtacc ccatgatcgc catattcaag ccgtacaaca
     1501 cctgcgccca gatctgcgtc tactgccagc ggaactggga gatcgaaggg gtcctctccc
     1561 ccgacgccat ggccccgccc gagaagatcg aggccgcctt ggactggttc agggaccatc
     1621 cgatggtaag cgaggtcctg atcacgggag gggacccagg agtgctctcg aacggacatc
     1681 tcaggagact tctgggaagg ctgatggagc taccgcacgt acgaaggctg cggctgggca
     1741 ccagggtgcc cgtggtcatg ccaatgagga tcgaccccgg cttccttgac ctaatggcgg
     1801 cggtccatga tccccccgga agggagttcg cgctcgtgac ccatttcgag catccatatg
     1861 aagtgacccc ggaagcggcc gaggccgtgc gctccctgcg aacgcttggc atttccgtct
     1921 acaatcaaca ggtgttcact atggagaact gccgtcgctt cgagacggtg gcggtgcgct
     1981 cgacgctcaa aaggatcgga gtggacccct actacacctt caatgccaag gggaaggagg
     2041 agacgtccga gtacagggtg cccatatcaa ggctcctcca ggagcgcaag gaggaggcaa
     2101 ggctactacc tggcctgagc cgaacggacg aaccggtgtt caacatcccg gcgctgggaa
     2161 agaaccatct gcgggcgtgg cagcaccacg acctgatcgc cattacccca aggggcgagc
     2221 gcgtgtacga gttccatccc tgggagaaga acattgccgt agcgcctacc tatgtgcaca
     2281 tggacgtgcc gatcctggac ttcctgatcg cccttgcgga gaggggagag gacgtgcggg
     2341 agtatgagag catatggtat tatttctagt accgctccgc cagtttggcg agcagctccg
     2401 acagcgaccc cttgccgatg tccatcgtgg cgatggccgc gatggcctcc cgcttgcgca
     2461 tgatcgggac ggctatgaca ggcagccccc ggtaaggacc gctggtggaa acgcgccgga
     2521 cgatcgcgcc ccgcttcatg gcctcctcca gcattgggcc ggtatagtgg gtgtcgatga
     2581 cctccccgtc ctcgcagcgc acccctggtc tgttccggct gcgcatcgtg accggcaggc
     2641 ggcccaggag ctcatggaca gcgagcgcca ggcagcgcag ctcatccgcc tcggtgtccc
     2701 ggctcaagag ttccgtgccc tcgttccagc agcggcggga caggttgctc agggcgaggg
     2761 cggcggtcac cgcccccttc tccgccgcat catagctgaa ggcggagccg ataattatca
     2821 catccccatc gtccatccgg aagtagttct tcaaaggggc gaccagtgat tggtccggga
     2881 actcctcgtc cccggggaag actattcggc cctggtcgac gaccagggtc gtggccccca
     2941 cggccccggc ccgcacggcc tcgtcccgtt gctcgcaccc caaccgcacg cggtcggcgg
     3001 cccccttgag cagcacggcg cagttg
//
LOCUS       DAOSYN010000162         3017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25602_length_3017_cov_3.240378, whole genome shotgun sequence.
ACCESSION   DAOSYN010000162 DAOSYN010000000
VERSION     DAOSYN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25602_length_3017_cov_3.240378"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PLJ11_06335"
     CDS             <1..926
                     /locus_tag="PLJ11_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04321.1"
                     /translation="LLGLLSSLNVAIPGMMPTADDPLAVLEAALPGTGFDPLTWIKAH
                     TGIWAERSVVETSGNVYGMLNYAVYAVDSEVHFADDIRTSTVTIPWVHITSLGEVVEH
                     NYTINVHTLNGIKAARSELYVTGSTIEVLDDALVFEASEGWVEGATLLAGDYDYFLRG
                     GSEVYNIATTYRTYKVEDSHSLNEGTWLTITAIDEGEPVANLTIYIRSANGEVVKTVV
                     TDAEGKARVLLPQYAWTLDGKDDGFNPYTINATFGSGEASMEVTLNQSYMDVTLEGEK
                     ESNIGAILAVVGVLVILLLIVAAVVVMRRRK"
     gene            1025..2317
                     /gene="gatD"
                     /locus_tag="PLJ11_06340"
     CDS             1025..2317
                     /gene="gatD"
                     /locus_tag="PLJ11_06340"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOO04322.1"
                     /translation="MSYSERARELLARSRAGEGDTVRLRAGGREYMGILMPHHGFSHP
                     DVIVIKLSSGYNIGVRVDDGSELTVVAKAEARASRPMPPQPSNGLPPVSFLGTGGTIA
                     SYVDYRTGAVHPALRAEELVATVPELAGICVPRSRVILSMFSENMDVRSWQTLAEAVA
                     DELNAGAEGVIVPHGTDTLGYTSAALAFMLGDVPRPVVLVGAQRSSDRPSSDAYGNLL
                     SSARFCVQADAAEVFVLMHGETSDTFAHVHRGTKARKMHTSRRDAFQSINAPPVARVD
                     IEGGLELLAPCRPKGRGGVRPELAMEEEVGLLYFHPGMRPELVRKVAEGLRGLVVAGT
                     GLGHVSQGVVTILREIVSQGKPVVMTSQCLGGRVNLNVYDTGRDLLSAGVIPGEDMLP
                     ETALVKLMWVLGRTDNMEEVARMMTTDLRGEISERREL"
     gene            2317..>3017
                     /gene="gatE"
                     /locus_tag="PLJ11_06345"
     CDS             2317..>3017
                     /gene="gatE"
                     /locus_tag="PLJ11_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOO04323.1"
                     /translation="MYPVTCGLEVHQQLDTRKLFCSCRSQLVEGQGARFVRRLRPTQS
                     ELGEIDRAALAQAERGMRFTYQAPPGVSCLVEADEEPPHAADAEAVQAALVVSALLGC
                     QVMDEVHFMRKIVIDGSNTAGFQRTALVAVDGALEVDGRRIGIASLCLEEDAARKVDA
                     KDREVTYRLDRLGIPLIEIATAPGMHDPEEVREVAQRLGSILRSTRKVKRGLGTIRED
                     LNISVPGGARVEIKGA"
BASE COUNT          531 a          989 c         1029 g          468 t
ORIGIN      
        1 cgctcctggg gctcctctcc agcctgaacg tggcaatccc tggcatgatg ccgaccgcgg
       61 acgacccatt ggccgtcctc gaggccgcgc tgcccggcac tgggttcgac ccattgacgt
      121 ggatcaaggc gcacaccggg atatgggccg agcgctccgt ggtggagacc agcgggaatg
      181 tgtacgggat gctgaactac gccgtctacg ccgtggacag cgaggtccac ttcgcggacg
      241 acatcaggac gagcacggtc accatcccct gggtgcacat cacgtccctg ggggaggtgg
      301 tggagcataa ctacaccata aacgtccaca ccctgaacgg catcaaagcc gcccgctccg
      361 agctgtacgt gaccggcagc accattgagg tgctcgacga tgcgctggtg ttcgaggcct
      421 ccgagggctg ggtggagggc gccacgctcc tggcgggcga ctatgactac ttcctccgcg
      481 gagggtccga ggtctacaac atcgccacca catataggac gtacaaggtc gaggactccc
      541 acagcctgaa cgagggcact tggctgacca tcaccgcgat cgacgagggc gagccggtgg
      601 ccaacctgac catctacatc aggagcgcca acggggaggt cgtcaagacc gtggtgaccg
      661 acgccgaggg caaggcccgc gtgctcctcc cccagtacgc ttggacgctc gacggcaagg
      721 acgacggctt caacccctac accatcaacg ccaccttcgg gtccggagag gcgtccatgg
      781 aggtgaccct gaaccagtcc tatatggacg tcaccctcga gggcgagaag gagtcgaaca
      841 tcggcgcgat cctggcggtg gtcggggtcc tggtcatcct cctgctcatc gtagccgctg
      901 tcgtggtcat gaggcgccgg aagtaaggcc caaacccctt cggtccaaac ctcttcccct
      961 ttctccattt ttccatggct aggatacctt gttaaagagg gagccccatt cctgacagag
     1021 cgcaatgagc tattccgaga gggccaggga gctattggcc cgcagcaggg cgggtgaggg
     1081 ggacaccgtg cgcctcaggg ccggagggag ggagtacatg ggcatcctga tgccccatca
     1141 cgggttcagc caccccgacg tcatcgtcat caagctgagc tctgggtaca acatcggcgt
     1201 gagggtggac gatggctcgg agctcacggt ggtggccaag gctgaggcca gggcctcgag
     1261 gccaatgccc ccgcagccat ccaacggcct gccccccgtc tccttcctcg ggaccggggg
     1321 cacgatcgcg agctacgtgg actatcgcac gggggcggtc catcccgcgc ttagggccga
     1381 ggaactggtg gccaccgtcc cagagctggc cgggatatgc gtcccgcgct ccagggtgat
     1441 cctctccatg ttcagcgaga acatggacgt caggagctgg cagaccctgg cggaggcggt
     1501 ggccgacgaa ctgaacgccg gtgcggaggg cgtgatcgtc ccccacggga ccgacacctt
     1561 gggctatacc tctgccgccc tcgccttcat gctcggggat gtgccccgcc cggtcgtgct
     1621 ggtgggggcc cagcgctcct ccgaccgccc ctcctccgat gcatacggca acctcctctc
     1681 ctcggcgagg ttctgcgtcc aggcggacgc ggccgaggtc ttcgtgctca tgcacggcga
     1741 gacctccgac accttcgccc acgtgcaccg cggtacgaag gcccggaaga tgcacaccag
     1801 ccggagggat gccttccaga gcatcaacgc cccgcccgtc gcccgggtcg acatcgaggg
     1861 cggcctggag ctgctcgcgc cctgcaggcc caagggcagg ggcggcgtgc ggccggagct
     1921 ggcaatggag gaggaggtcg ggctcctgta cttccatccc gggatgaggc cggagctcgt
     1981 caggaaggtc gccgagggcc tccgcggcct ggtcgtggcc gggacggggc tcgggcacgt
     2041 atcccagggc gtcgtcacca tcctcaggga gatcgtctcc cagggcaagc cggtggtcat
     2101 gacctcccaa tgcctggggg gcagggtcaa cctcaacgtg tacgacaccg gaagggacct
     2161 gctctccgcg ggggtcatcc caggcgagga catgctgcca gagaccgccc tggtcaagct
     2221 gatgtgggtg ctcggacgca ccgataacat ggaggaggtg gcgaggatga tgaccaccga
     2281 cctgcgcggg gagatctcgg aaaggaggga gctgtgatgt acccggtcac ctgcggcctc
     2341 gaggtccatc agcagctgga cacccgcaag ctcttctgct cctgccgctc ccagctggtg
     2401 gaggggcagg gcgcgcggtt cgtccgccgc ctccgtccga cccaaagcga actgggagag
     2461 atcgacaggg cggcgttggc gcaggcggag cgggggatgc gcttcacgta ccaggcaccg
     2521 cccggcgtct cctgcctcgt cgaggccgac gaggagccgc cgcacgccgc ggacgcggag
     2581 gccgtgcagg ccgccctcgt cgtctcggcc ctgctcggct gtcaggtgat ggacgaggtc
     2641 catttcatgc gcaagatcgt gatcgacggc tccaacaccg ccggcttcca gaggaccgcc
     2701 ctggtggcgg tcgacggggc gctcgaggtc gacggcaggc gcatagggat agcatcgctg
     2761 tgcctggagg aggacgccgc ccgcaaggtt gatgctaagg acagggaggt cacctatcgc
     2821 ctggaccggc tcggcatccc gctcatcgag atcgccaccg ccccgggcat gcacgacccg
     2881 gaggaggtga gggaggtggc ccagcgcttg ggttcgatcc tccgctccac ccgcaaggtg
     2941 aaaagggggc tggggaccat ccgggaggac ctcaacatat ccgtgcccgg gggcgcgcgc
     3001 gtggagatca agggcgc
//
LOCUS       DAOSYN010000163         3012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25656_length_3012_cov_3.941495, whole genome shotgun sequence.
ACCESSION   DAOSYN010000163 DAOSYN010000000
VERSION     DAOSYN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25656_length_3012_cov_3.941495"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1071
                     /locus_tag="PLJ11_06350"
     CDS             232..1071
                     /locus_tag="PLJ11_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04324.1"
                     /translation="MVENRADIPSLSRLCLASQLLRGTEGRVIPVMAAKSFSDRTKVI
                     AERAGIRTVTLPRSVQLDRRPREGKGPTPKLTSAKAWRVVATLIGQPSASIRSIALKA
                     GVSYGWAYAVCMHLLDTDLARRKGNRVVLEDVDRLLSGAAWERPLKALMVDEFRTDMD
                     DVFGAAREISGMLSDNKQRHAFAGYLAGALYTGHAVRFDMVHLYIPMAEASTLREIYS
                     NDVGGGITVQVLVPDRDVYSESRRIEGIQTVSPAQALLDLAGVGAGALDMSKALVRSY
                     ADL"
     gene            1058..1810
                     /locus_tag="PLJ11_06355"
     CDS             1058..1810
                     /locus_tag="PLJ11_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04325.1"
                     /translation="MPTYDPLPVIGASLDELRFIASLVVQYEQRDSPRTVLIGGWAVH
                     SYNAWYGSIDIDLITDAKGKKRLIDRLVNERGFERFRMDDDLRGLRKNVANNQWVIID
                     LGTRGKHDPFEGTGYYLDYTLLDGNIEWREVTRGAVLPVPERTTLLVLKAKAAWDRNY
                     RLRNGTSPDSAWERGKLHKDRGDILALLDPTKGGKDVRIDRLAKPLEGWPFLWECIVG
                     ALRDAAAVRSYGWLTAKEAEGLATGLGGLLGR"
     gene            1903..>3012
                     /locus_tag="PLJ11_06360"
     CDS             1903..>3012
                     /locus_tag="PLJ11_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO04326.1"
                     /translation="MSDWRSELPRGAIEVLEEDGITTLYPPQERAVPLALQGKSIVLA
                     VPTASGKSLVAYLAAVKRALHEGGKVLYIVPLRALASEKLEDLRRFERLGVRVGMSIG
                     DYDEADREIDQLDILVATSEKADALMRHRPEWVRSLGLVIADEVHLMNDPERGPTLEI
                     TLTKLRMLNPGLQVIALSATVGNSKEMAEWLGAEHIAMDWRPVRLKEGVLLGDTIEFT
                     DGTERTVRPLDDQVWSLVKDTLDEGGQALVFVNTRRSTETLAAKFSRLSKGTAADMGK
                     ELDEAFQDDHTSLGSALKDCIRHGMAFHSAALSNEQRRTVERLFKAGKVRCIVATPTL
                     AAGINLPARRVVVRDVNRFDDGGWVPLPVMEVKQMC"
BASE COUNT          602 a          830 c         1026 g          554 t
ORIGIN      
        1 atcactgatg atcgttcaca tgccagttgt catttttcag ctgttatcag tgataactat
       61 atatagttat cacttaatca taataatgaa gatatgaata attcgctaga tgaatacatt
      121 agaaaaggcc taggacttcc ggagaagggt caagccaggc gtatcgacct agacgagtct
      181 gacctagccc cggacctggc cattgaaacg cctgagggcc tgatcgtcgc aatggtcgag
      241 aaccgagccg atattccatc cctgtccagg ctttgtctcg cctcccaatt gctgagaggg
      301 accgagggga gggtgatccc ggtgatggcc gccaagagct tcagcgatag gacgaaggtt
      361 atcgcggaaa gggctggcat ccgaacggtc actttaccga ggtctgtaca gctggatagg
      421 cggccgcggg aagggaaggg cccgacgcct aagcttacct cggccaaggc ctggcgggta
      481 gtggcaacgc tgatagggca gccttctgca tccataagaa gcatcgcctt gaaagcaggg
      541 gtttcttatg ggtgggcata tgctgtgtgc atgcacctgt tggacaccga ccttgcccga
      601 aggaaaggga accgtgtggt cttggaggat gtcgaccgat tgctgagcgg cgcggcctgg
      661 gagaggccgc tcaaggcatt gatggtggac gagttcagga ccgacatgga cgatgtgttc
      721 ggagcggcaa gggagatcag cgggatgctg agcgataata agcagcgcca cgccttcgct
      781 ggttacttgg ccggcgcatt gtacacaggc catgcggtcc gtttcgatat ggtgcatctg
      841 tacatcccca tggcagaggc ttcgaccttg cgggagatct attccaatga cgtgggggga
      901 gggatcaccg ttcaggtatt ggtgcccgat cgcgatgtgt actcggaatc gcgcaggata
      961 gagggcattc agaccgtttc ccctgctcag gcgctgctcg acctggccgg ggtgggggct
     1021 ggggctttgg atatgagcaa ggccttagtg aggagctatg ccgacctatg atcctttacc
     1081 tgtgatcggc gcctctttgg acgagctgcg cttcatcgcg agccttgtcg ttcagtatga
     1141 acagcgggat agcccccgga ccgtgctgat cgggggatgg gcggtccatt cctacaatgc
     1201 ctggtacggg tctattgata ttgatcttat aaccgacgcc aaagggaaaa agaggcttat
     1261 agaccgcctt gtcaatgaga gaggcttcga gcggttcagg atggatgatg acctcagagg
     1321 ccttcgtaag aacgtagcga acaatcaatg ggtcatcatt gatcttggaa cgaggggtaa
     1381 gcacgatccg ttcgaaggca cgggatatta tcttgattac acccttcttg atgggaacat
     1441 tgaatggagg gaggtgacgc gtggtgccgt gcttcccgtt ccagagagaa cgacccttct
     1501 ggtcctcaag gccaaggccg cctgggaccg gaactatcgt ttgaggaacg gcacctctcc
     1561 agacagcgca tgggagaggg ggaagctgca caaggaccgc ggggacatcc tggccctttt
     1621 ggacccgacc aaaggaggta aggacgtgcg catcgaccgg ctcgccaagc ccttggaagg
     1681 atggccattc ctttgggaat gtattgttgg agctcttcgg gacgccgccg ccgtccggtc
     1741 ctatggatgg ctgaccgcga aggaggctga ggggcttgcg accgggctgg gagggctgct
     1801 gggaagatga tgccgaggcc ggaggggcgg cccctcggcc ccgctgatcg cccgggcccg
     1861 attgaaaatc gtaataacca gggagcgcat cccctatcac ccatgtccga ctggaggagc
     1921 gagctgccgc ggggcgcgat cgaggtgctg gaggaggacg gcataacgac gctctacccc
     1981 ccgcaggaga gggcggtgcc cctggcgctg caagggaaga gcatcgtgct cgccgtgccc
     2041 acggcctccg ggaagtcgct ggttgcttat cttgccgcgg tgaagcgcgc gctgcacgag
     2101 ggcggcaagg tcctctacat cgtcccgctg cgcgccctgg cgtccgagaa gctcgaggac
     2161 ctcagacggt tcgagcgcct gggcgtccgg gtcggcatgt ccataggcga ctacgacgag
     2221 gcggaccggg agatcgacca gctggatatc ctggtcgcca cctcggagaa ggcggacgcg
     2281 ctcatgcgcc accggcccga gtgggtgcgc tccctcggcc tcgtgatcgc ggacgaggtg
     2341 cacctgatga acgacccgga gcgggggccg acgctcgaga tcacgctgac gaagctccgc
     2401 atgctcaacc ccgggctgca ggtgatagcc ctctcggcca ccgtcggcaa cagcaaggag
     2461 atggccgagt ggctgggggc ggagcacatc gccatggact ggcggccggt gaggctgaag
     2521 gagggggtgc tcctcgggga caccatcgag ttcacggacg gcacggagcg cacggtccgg
     2581 ccgctggacg accaggtgtg gtccctggtg aaggacacgc tggacgaggg cggccaggcg
     2641 ctggtgttcg tgaacacccg gaggtccacc gagacccttg cggccaagtt ctcccggctg
     2701 agcaagggga cggccgccga catggggaag gagcttgacg aggccttcca ggacgaccac
     2761 acctccctcg gctccgcgct caaggactgc atccggcacg ggatggcgtt ccacagcgcc
     2821 gcgctgagca acgagcagcg gcggacggtc gagcgcctct tcaaggccgg gaaggtcagg
     2881 tgcatcgtcg ccaccccgac cctggccgcg gggatcaacc tcccggcgcg gcgcgtggtc
     2941 gtgcgggacg tgaaccgctt cgacgacggg ggctgggtgc cgctgccggt gatggaggtc
     3001 aagcagatgt gc
//
LOCUS       DAOSYN010000164         3000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25769_length_3000_cov_3.867572, whole genome shotgun sequence.
ACCESSION   DAOSYN010000164 DAOSYN010000000
VERSION     DAOSYN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25769_length_3000_cov_3.867572"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLJ11_06365"
     CDS             <1..764
                     /locus_tag="PLJ11_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOO04327.1"
                     /translation="AIVFLVLAGLINAISYFLSSKIVLWSYRVRLVSEAEAPRLYRTV
                     RNIAAMNGMPMPKIGIIPTSTPNAFATGRNPKNAVVCATEGLLRILDDEELSGVMAHE
                     MAHVKNRDILVMSVASTIAGAISFAARFAYYGALFGDRRNEGGLLVGILIAITAPIAA
                     MIVQLAISRNREYKADYEGAKLIGRPLYLARALEKLEAANRRDPLKFGNPASSNMFIV
                     NPFRGGGMVTLFMTHPPMEERIKRLRQMASKMGQY"
     gene            complement(769..1470)
                     /locus_tag="PLJ11_06370"
     CDS             complement(769..1470)
                     /locus_tag="PLJ11_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO04328.1"
                     /translation="MTPRPNLHTHTLHSDGDFTVEEVVRAAARAGLTHVAITDHFETS
                     KVAHPLRRASLERYIEEVRAVGRAYEGRVNVLAGVEIDTNPERCALSELPFALLNRLD
                     LVLFEYVEDPLQGGVPLSELRPLCEALTVPFGLCHWDMDRIFRGRRPEWLADRLSALG
                     AFVEVPTSQHYARDGRFLYEHSERFYRAFAGKVRVSIGTDTHHALEEVGNIGRGMEFL
                     RANGLCGQLLFNGQG"
     gene            complement(1557..2855)
                     /locus_tag="PLJ11_06375"
     CDS             complement(1557..2855)
                     /locus_tag="PLJ11_06375"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOO04329.1"
                     /translation="MKPVLQVALDLMHLKRALEIARESLEGGADWIEAGTPLIKSEGA
                     ECLRALKREFPGRTLVADMKTMDVGAFEVEIAAKAGADIVTVLGLSDDGTISESVLTA
                     RQYGAAIMLDLINVPDKVARARRAEELGVSYICLHVGIDEQMKGEGSPLDVVSEVAAA
                     VSVPVAVAGGITAETAPRVVERGAAIVIVGGGIIKTGDVRAAARAVKDAMATGKGLPN
                     ALARKYGQEELFEAFSKVSTCNVADAAHKRGVMDNGIVLRTGHGVKAVGRALTVQTAK
                     GDWAKPVEAIDRAKKGDVIVIDAGGGEIAVWGELAANSALVKGVAGVVIDGAARDIDG
                     IVDLGFPCFSRHVSPHAGEPKGFGGIGHEIVCGGQMVRTGDWIVGDESGVIVIPQEHA
                     VELANRAVDVFEREERVREEIKRGGTLSSVQELEKWEQVK"
BASE COUNT          478 a         1106 c          928 g          488 t
ORIGIN      
        1 gggcgatcgt gttcctcgtg ctggccgggc tgatcaacgc gatatcgtac ttcctctcca
       61 gcaagatcgt cctctggtcg taccgagtgc ggttagtcag tgaggcggag gcgcctcggc
      121 tctaccggac cgtccgcaac atcgccgcca tgaacggcat gcccatgccc aagatcggca
      181 tcatcccgac gagcacgccc aacgccttcg cgacggggcg caacccgaag aacgcggtgg
      241 tgtgcgccac ggagggcctg ttgcgcattc tggacgatga ggagctctcg ggcgtgatgg
      301 cgcacgagat ggcccacgtg aagaaccggg acatcctggt catgagcgtg gcgagcacca
      361 tcgccggggc catctccttc gccgcgcgct tcgcgtacta cggcgcgctg ttcggcgacc
      421 gccggaacga gggcgggctg ctggtcggca tcctgatcgc gatcacagcc ccgatagcgg
      481 cgatgatcgt gcagctggcc atctcccgca accgggagta caaggccgat tacgagggag
      541 cgaagctcat aggcaggccg ctgtacctgg ccagggccct ggagaagctg gaggcggcca
      601 accggcgcga ccccctcaag ttcggcaacc ccgcgtcctc gaacatgttc atcgtcaacc
      661 cgttccgggg aggcgggatg gtcaccctgt tcatgaccca tccgcctatg gaggagcgga
      721 tcaaacggct gcggcagatg gccagcaaga tgggccagta ctgaaccctc atccctgccc
      781 gttgaacagc agctgcccgc acagcccgtt cgcgcgcagg aattccatcc cccggccgat
      841 gttgcccacc tcctccaacg catggtgcgt gtccgtccct atcgacaccc tcaccttccc
      901 ggcgaaggcg cggtagaagc gctccgagtg ctcgtaaagg aagcgcccgt cccgggcgta
      961 gtgctgggag gtgggcacct ccacgaaggc gcccagggcc gagagccggt ccgccagcca
     1021 ctccggccgc ctgccccgga agatgcggtc catgtcccaa tggcagaggc cgaaggggac
     1081 ggtcagcgcc tcgcagagag ggcggagctc ggaaaggggg acgccgccct gcagcgggtc
     1141 ctccacgtac tcgaacagga cgaggtccag ccggttgagg agggcgaagg gcagctcgga
     1201 gagggcgcac cgctccgggt tggtgtcgat ctccacgccc gcgagcacgt tgacccttcc
     1261 ttcataggcc ctccccacag ccctgacctc ctcgatgtag cgctcgaggg aggcccgccg
     1321 cagggggtgc gcgaccttgc tcgtctcgaa gtggtccgtg atcgcaacgt gcgtgagccc
     1381 ggcgcgcgcc gcggcccgca ccacctcctc gaccgtgaag tccccgtcgg agtggagcgt
     1441 atgcgtatgc aggttcggcc ggggggtcat cgacccgtcc tatggcgctc ccgatataaa
     1501 gcattgggac ggcggaagcg atggccgacg gaggacgttg accgccctag gcgccctcac
     1561 ttgacctgct cccacttctc cagctcctgg acgctcgaga gggtgccgcc ccgcttgatc
     1621 tcctcccgga cgcgctcctc ccgctcgaac acatcgacgg cccggttcgc gagctcgacc
     1681 gcatgctcct gcggtatgac gatgacgccg ctctcgtccc cgacgatcca gtccccggtc
     1741 cgcaccatct gccccccgca cacgatctcg tgcccgatcc ccccgaaccc cttgggctcc
     1801 ccggcgtgcg gggaaacgtg cctgctgaag caggggaacc ccaggtcgac gatcccgtcg
     1861 atgtcgcggg ccgcgccgtc gatgaccacc ccggccaccc ccttcaccag ggcgctgttg
     1921 gccgccagct ccccccagac cgcgatctcg ccccctccgg cgtcgatcac gatgacgtcc
     1981 cccttcttcg cgcggtcgat cgcctccacc ggtttggccc aatccccctt ggccgtttgg
     2041 acggtgagcg ccctcccgac ggccttcacc ccgtggccgg tgcggagcac gatgccgttg
     2101 tccatgaccc cgcgcttgtg cgcggcatcg gcgacgttgc aggtggacac cttcgagaac
     2161 gcctcgaaca gctcctcctg cccgtacttc ctggccaggg cgttcgggag ccccttcccg
     2221 gtggccatgg cgtcctttac cgccctggcc gccgcccgga cgtcccccgt cttgatgatc
     2281 cccccgccga cgatgacgat cgccgccccg cgctcgacca cccgcggggc cgtctccgcg
     2341 gttatgcccc cggccacggc caccgggacg ctgacggccg cggccacctc cgagaccacg
     2401 tccaacgggg agccctcccc cttcatctgc tcgtctatcc ccacgtgaag gcagatgtac
     2461 gagacgccga gctcctcggc ccgcctggcc cgggcgacct tgtcagggac gttgatcagg
     2521 tccagcatga tcgccgcccc gtactgccgg gcggtgagca ccgattccga gatggtgcca
     2581 tcgtccgaga gccccaggac ggtcacgatg tccgcgccgg ccttggccgc gatctccacc
     2641 tcgaaggccc cgacgtccat ggtcttcatg tcggcgacca gggtcctgcc ggggaactcc
     2701 ctcttcaggg cgcgcaggca ctccgccccc tcgctcttga tgagcggcgt gcccgcctct
     2761 atccagtccg cccctccctc cagggattcc cgggcgatct ccagcgcgcg cttaagatgc
     2821 atcaggtcca aggcgacctg caggaccggc ttcatggaac ggcaatctcc gccaggatac
     2881 taatcctttc ctaaaacaat atatccgcat aggggcatca tcatcgtatg gccgcatccc
     2941 cgggcaccaa gctctgcacc ggcatggagt gcatagaccg cctggtggga ggggggctcc
//
LOCUS       DAOSYN010000165         2980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25947_length_2980_cov_4.755897, whole genome shotgun sequence.
ACCESSION   DAOSYN010000165 DAOSYN010000000
VERSION     DAOSYN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25947_length_2980_cov_4.755897"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..644
                     /locus_tag="PLJ11_06380"
     CDS             180..644
                     /locus_tag="PLJ11_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOO04330.1"
                     /translation="MKVALVYDSVSSAKTTAKVAEAIAAALRGKGLEVASLPVEDAKH
                     IRVEDYDALIVGTPTMAWAPTRGITGFLNGLKGEGLRGKKAASFDTQLRSLLSGNANR
                     AMESRLKKMGFEIACPHLLSYVKHESGTYQLLDGEVQKAEGWAEGLADALLR"
     gene            706..2088
                     /locus_tag="PLJ11_06385"
     CDS             706..2088
                     /locus_tag="PLJ11_06385"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOO04331.1"
                     /translation="MVVRSINPRTGQVIKEFVPSAPDDVMVALHNGRKAQQKWMSGWD
                     KEERLAAVMELQSALLAMKDDLIATETLEGGFPRNDVIGTYNGVIKGFDHYAAEARAI
                     NDYEFPLDQAAWPHTKATVNLVPHGVIGHIGIWNYPFWQTMITLIPALMAGNAVVFKP
                     SELTTMTGLKIKEAFGRTSMPEDLFQVLIGGADIGKTIVAAPFDALVFTGGRSTGLDI
                     TRNAGIKPMVLELSGNDPGIVCSDVNVPAAARGVASGAFSHGGQVCIRVKRLYVVESV
                     AEQFIEELLNVVARIDVEHQIGPLIREEARAKVHEKVHRAVQAGCELLTGGENLPGLG
                     YFFEPTVLRVFDDSLEIIREETFGPVLPIRVVKDEEEAIRLANDSDYGLGATVWTSDP
                     QKGESIARRLEAGNIWINEHGRTVNCGELFQGWKCSGIASTNRRITLFMKKRMIVNHM
                     SSEPRPYWFK"
     gene            complement(2171..2638)
                     /locus_tag="PLJ11_06390"
     CDS             complement(2171..2638)
                     /locus_tag="PLJ11_06390"
                     /inference="COORDINATES: protein motif:HMM:NF021422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 family protein"
                     /protein_id="HOO04332.1"
                     /translation="MAKVEGISGLTKLFRESLNDVDEGSHVLFIGSEAVCPPFASLLA
                     YAVRDHDLHFGFCPKAVWEKSRVMNWVDGAGFQIGKGQFEPGEADVIVLLGGLAMPKF
                     GCPVEDVKALLSQVPRCKLVIGMGFMDVFRRAGWDKVIHFDALIDCYMSADIV"
BASE COUNT          694 a          831 c          872 g          583 t
ORIGIN      
        1 cgccttatct cgggataatc ctcgtggccg tgatcgttgt ggtcgtggtc gctgcggtgc
       61 tcatcgtccg gcggaagtaa cctaaacctc tttccaatct tttttttgat ccttccctca
      121 tcgatatgac gaagttatat acggtccaag gccgtagggt tcgatgagaa ggggatggca
      181 tgaaggtagc cctggtctat gattccgttt cttcggccaa gacgaccgcc aaggtcgccg
      241 aggcgatcgc cgccgccctt cgagggaaag ggctggaggt ggcctccctc ccggtagagg
      301 atgcgaagca catccgggtg gaggattatg acgctctgat cgtcggaacc cccacgatgg
      361 catgggcgcc cacccggggc atcaccggtt tcctgaacgg gctgaagggc gaggggctca
      421 ggggcaagaa ggcggcgagc ttcgacacgc agctgaggtc cctcctgagc ggcaacgcca
      481 acagggcaat ggaatccaga ctgaagaaga tgggtttcga gatcgcatgc ccgcatcttc
      541 tttcatacgt caagcacgaa agcggaacgt accagctctt ggatggggag gtccagaagg
      601 ccgagggatg ggccgaggga ctggccgacg ccctgctgag atgaccaaac cccgacgcgg
      661 tcctggggca acaaccctaa aagcccgaac gcgaatagta ttctcatggt agtccgttcc
      721 atcaatcccc gcacggggca ggtgatcaaa gagttcgtcc cgagcgcccc cgacgacgtt
      781 atggtcgctc ttcataacgg ccgtaaggcc caacaaaagt ggatgtccgg atgggacaag
      841 gaggaaagat tggccgctgt catggagctg caatccgccc tattggcaat gaaggatgac
      901 ctgatcgcca cggaaacgct cgagggcggc ttccccagga acgacgtcat tggcacgtac
      961 aacggcgtga tcaaggggtt cgaccattac gctgccgagg ccagggcgat caacgattac
     1021 gagttccccc tggaccaggc ggcctggccg cacaccaagg cgacggtcaa cctggtcccg
     1081 cacggcgtca ttggccacat aggcatctgg aactatccct tctggcagac gatgatcacg
     1141 ctcatcccgg ccctcatggc cgggaacgcc gtggtgttca agccctccga gcttaccacc
     1201 atgacagggc tcaagatcaa ggaggcgttc gggcgcacca gcatgcccga ggacctgttc
     1261 caggtgctca tcggaggggc ggacatcggc aagaccatcg tggcggcgcc cttcgacgcc
     1321 ctggtgttca caggcgggcg gagcactggc ctcgacataa ccaggaacgc tggtatcaag
     1381 ccgatggtcc tggagctctc cggcaacgat cccggcatcg tctgctcgga cgtgaacgta
     1441 cccgcggcgg ccaggggtgt ggccagcggc gcgttctccc atggagggca ggtctgcatc
     1501 cgcgtcaagc gcctgtatgt ggtggagagc gttgcggagc agttcatcga ggaactgctc
     1561 aacgtggtgg ccaggatcga tgtggagcat cagatagggc cgctcatcag ggaggaggcc
     1621 cgggcaaagg tccatgagaa ggtgcacagg gcggtccagg ctggttgtga gcttctcacc
     1681 ggcggggaaa accttcccgg gctgggctac ttcttcgagc ccacggtcct cagggtcttc
     1741 gatgattccc tggagatcat tcgcgaggag accttcgggc cggtgctgcc gatccgagtc
     1801 gtcaaggacg aggaggaggc catcaggctg gcgaacgaca gcgactacgg cctgggcgcg
     1861 accgtctgga ccagcgaccc gcagaaaggc gagagcatcg cccgaaggct ggaggcgggg
     1921 aacatctgga tcaacgagca tgggcggacc gtgaactgcg gggagctttt ccagggatgg
     1981 aagtgcagcg ggatcgcgag caccaaccga cggatcaccc tcttcatgaa gaagcggatg
     2041 atagtcaacc acatgtcctc cgaacccagg ccctattggt tcaagtgagg gtattggaag
     2101 gcagccctgc tcacagcccc ctgggatgcc agggggtcgc aaggttccgt aaggaccgac
     2161 gggatccaat tcagacgatg tccgcggaca tgtagcagtc gatgagggca tcgaaatgga
     2221 tgaccttgtc ccatccagcc cttcggaaga cgtccatgaa ccccattccg atcaccagct
     2281 tgcacctcgg gacctgggac aggagcgcct tgacatcctc gaccgggcat ccgaacttgg
     2341 gcatggccag gccgcctaat agcactataa catccgcctc tccaggctcg aactgcccct
     2401 tgccgatctg aaagccagcc ccgtcgaccc agttcatcac ccgacttttc tcccaaacgg
     2461 ccttgggaca gaaaccgaag tgcaggtcgt ggtcccggac cgcatatgct aacaggcttg
     2521 cgaaaggggg acagaccgcc tcagagccga tgaaaagtac gtggctcccc tcatcgacat
     2581 cgttcaacga ttccctgaac agcttggtaa ggcctgagat gccttcgacc ttggccatgt
     2641 gtgataaagg ctagagctat caaataatta tttcgaatgt ggttattaat aatttatatc
     2701 aaatatatat caaccacccc ctggtctgtg attggactgt ggaatagacg cctgaggatt
     2761 ggtacataat tcaattaata tatagaatat tataatatta atatagtata taatagttca
     2821 acgaatcata aatattaaat cttattactt aatcagtatt attaatatag caaataactt
     2881 tacaaaaact cctcacctac caaagcaacc gatacaaaat ccattaatcc tgagttcgac
     2941 agttgaccgg gcgaaatttc actgatttta gatgttgaca
//
LOCUS       DAOSYN010000166         2976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_25986_length_2976_cov_5.048271, whole genome shotgun sequence.
ACCESSION   DAOSYN010000166 DAOSYN010000000
VERSION     DAOSYN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25986_length_2976_cov_5.048271"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="PLJ11_06395"
     CDS             complement(<1..1338)
                     /locus_tag="PLJ11_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO04333.1"
                     /translation="MVAAISRKRFLTGWTAFIILFFLFFVILPTIYILSYAFTEWGSI
                     EALLEDQGRMDLIWGAIGASFQIAGIVTVIDIVTGLPMAWMLVRKDFRGKNLLDTLID
                     MPLAVPTAALGFSSAMFFAITDESLQAPSFALGTIGSPYLLIILLHVVFSYPYMVRSL
                     SAILVQIDQTYETAGRTLGASPLTAARTITLPLFRAGLVTGIILCFARSLSETGGTMI
                     ALATMANARGFATGPTLIGEWKDALDSQPDLAAPLAFVSILLILLSLALLVVIKLLVM
                     RVHLPMGRIWPRPERLLSRGVFPKVKDGLAVLFLVVFVLVPSFFIFTYVGSVEPTAFD
                     WAPFIRSLLYSFLIAGVVTGIDIALGVPLALYIAKNRSGRLAGALDVLVNVPLIVPTA
                     ALGYSLGVFWASQEIVPTHDVLLIVLAHVAFTYPLVVRNVAGAVEEVGPAYEET"
     gene            complement(1410..2393)
                     /locus_tag="PLJ11_06400"
     CDS             complement(1410..2393)
                     /locus_tag="PLJ11_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOO04334.1"
                     /translation="MKALILAGGMGTRLRPLTLSTPKPLLPLLDRPMVMHIIDSLPAE
                     VDTVVLAVSYMKDLLEGYFLKHDCGRKVVLVNEEEPLGTGGAVRNVRQYLDGTFLCFN
                     GDIISSLDLNDMLAFHRRHGGIGTLALWRVQDPSAFGVVGLEGARITVFQEKPAPGEA
                     ISDLINAGVYVFEPELLEHIPPGVVSLERQVFPNVLDRGLYGYRFDGHWVDCGTPAGY
                     LEAQRALLERGRTKVAPAIVRGSELIGPNYIDGASLSGCRVGPFVCALPGASIGEGST
                     VEGSVLMEGATIGRGCTVRACLLGPGTVVKDGSALQGRALAPLRGRQAGLH"
     gene            complement(2402..2617)
                     /locus_tag="PLJ11_06405"
     CDS             complement(2402..2617)
                     /locus_tag="PLJ11_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HOO04335.1"
                     /translation="MVMPLALMEKTLNKKVSLLLKDNRVLEGKLTGYDEYMNMVLEET
                     EERTADQTRRLGTVILRGNNVVSVAPL"
     gene            complement(2679..2855)
                     /locus_tag="PLJ11_06410"
     CDS             complement(2679..2855)
                     /locus_tag="PLJ11_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04336.1"
                     /translation="MALRGWAITRIPPKASSRYGDRVGPLSLVCVSDVVLQEDFVGCA
                     RSRLSEGQLLALKL"
BASE COUNT          569 a          933 c          966 g          508 t
ORIGIN      
        1 cgtttcctca taggcggggc ccacctcctc caccgccccg gccacgttcc tgaccaccag
       61 gggataggtg aaggccacgt gggccaggac tatgagcagg acgtcgtgcg tcgggacaat
      121 ctcctggctg gcccagaaca cgccgaggga gtagccgagg gcggcggtcg ggactatgag
      181 ggggacgttg accagcacgt ccagcgcccc ggccagcctt ccagagcggt tcttggcgat
      241 gtacagggcc aggggaacgc cgagcgctat gtcgatgccg gtgacgacgc ccgctatcag
      301 gaaggagtag agaaggctgc ggatgaaggg ggcccagtcg aaggccgtcg gctcgaccga
      361 gccgacgtag gtgaagatga agaaggaggg gacgaggacg aacacgacga ggaagagcac
      421 ggccaggccg tccttgacct tcgggaagac gccgcgggac aggagcctct ccggccgggg
      481 ccatatcctc cccattggca ggtgcaccct catgaccagc agcttgatca ccaccaggag
      541 cgccaaggag agcaggatga gcaggatgct cacgaaggcc aggggagcgg cgaggtccgg
      601 ttgggagtcg agggcgtcct tccactcacc gatgagcgtg gggcccgtgg cgaagcctct
      661 cgcgttggcc atggtggcca gcgcgatcat ggtccccccg gtctcgctta ggctgcgggc
      721 gaagcacagg atgatgcctg tgacaagtcc ggcgcggaac agcggcagcg tgatcgtcct
      781 ggctgcggtg agagggctcg cccccagggt gcgcccggcc gtctcgtatg tctggtctat
      841 ctgcaccagg atggccgaca gggagcgcac catgtagggg tacgaaaaga cgacgtgcaa
      901 caggattatc agaaggtagg gggagcctat ggtgcccagg gcgaaggacg gggcttgcag
      961 gctctcatcg gtgatggcga agaacatcgc gctggagaac ccgagcgcgg cggtggggac
     1021 cgccaggggc atgtcgatga gcgtgtccag gaggttcttt cccctaaagt cctttcggac
     1081 gagcatccag gccatgggca gtccggtaac gatgtcgatg accgtcacta tgcccgctat
     1141 ctggaaggag gcgcctatgg ccccccagat gaggtccatc ctcccctggt cctccagcag
     1201 cgcctcgatg gacccccatt ccgtgaacgc atagctcagg atgtaaatgg tgggcagaat
     1261 gacgaagaac aggaagaaga ggatgatgaa ggccgtccag ccggtgagga acctctttcg
     1321 gctgatcgcc gcgaccaagg gatcccattt gattctcgcc agaccttcac ctcgggaacg
     1381 gttggagaaa aggatgatgg aataaatacc tagtgcaatc ccgcctgcct acctcgaagg
     1441 ggggccaatg ccctaccctg aagggcgctc ccatccttca ccaccgtccc tgggccgagc
     1501 aggcaggcgc gcacggtgca tccccttcca atcgtggcgc cctccatgag cacggagccc
     1561 tcgaccgtgg agccctcccc tatggacgcg ccggggaggg cgcatacgaa cggccccacc
     1621 cggcacccgc tcagcgatgc cccgtcaatg tagttcgggc cgatcagctc ggagccgcgc
     1681 acgatggcgg gggcgacctt cgtccgccct ctttccagga gcgccctctg ggcctccagg
     1741 taccctgcgg gcgtgccgca gtccacccaa tgaccatcga accggtaccc gtagagcccg
     1801 cggtcgagca cgttcgggaa gacctgccgc tccagggaca cgaccccggg ggggatgtgc
     1861 tccagcagct ccggctcgaa cacgtacacg ccggcattga tgaggtcgga gatggcctcg
     1921 ccgggggctg gcttctcctg gaataccgtg atccgcgctc cctccagccc tacgaccccg
     1981 aaggccgaag ggtcctgcac cctccagagg gccagggtgc cgatgcctcc gtgccggcgg
     2041 tggaaggcca gcatatcgtt caggtccagc gagctgatga tgtccccgtt gaagcagagg
     2101 aaggtgccat ctaggtactg gcgcacgttg cggaccgctc cgccagtgcc caagggctcc
     2161 tcctcgttga ccaggaccac cttccggccg caatcgtgct tgaggaaata gccttcgaga
     2221 aggtccttca tatagctgac ggccaacacc actgtgtcca cttccgcggg cagggagtcg
     2281 atgatgtgca tgaccatcgg tctgtcgaga agcgggagca atggcttggg cgtcgagagc
     2341 gtcagaggcc tgagcctggt ccccatgccc ccggccagga tcaacgcttt catgaacgtt
     2401 atcagagcgg ggcgacgctg acgacgttat tgccccgcag gatgaccgta cccaggcgcc
     2461 gggtctggtc cgcggtgcgt tcctcggtct cctccagcac catgttcatg tactcgtcat
     2521 aaccggtgag cttaccttcc aggacgcggt tgtccttcag caggagggac accttcttgt
     2581 tcagggtctt ctccatgaga gcgaggggca ttaccattta gatcactgcc gtaggcatcg
     2641 tgcggtcaca tttaaacctt ttcgcctgga tggggccctc agagcttcag tgccaggagc
     2701 tggccttccg acagcctgct tcgggcacag cccacgaaat cctcctggag gaccacatcg
     2761 gagacgcata ccagggacag cgggccgacg cggtcgccgt acctggagga cgccttgggc
     2821 gggatgcggg tgatggccca accccgaagg gccattccta ttgctctctc tgcttcgatt
     2881 ccatccactc cttgatctct tccttcgtcg ttcccagccc gaacctctcg gagaactgcc
     2941 gggtggtgct gagcaggagc gactgcccca tatgct
//
LOCUS       DAOSYN010000167         2972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_26027_length_2972_cov_2.938293, whole genome shotgun sequence.
ACCESSION   DAOSYN010000167 DAOSYN010000000
VERSION     DAOSYN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26027_length_2972_cov_2.938293"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLJ11_06415"
     CDS             complement(<1..663)
                     /locus_tag="PLJ11_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOO04337.1"
                     /translation="MRDKVGLLLPTALIVLAEGLFFNGRTEACLEVHALNIFLCILLP
                     IWAEKQMLLYQSFSLVSLLRVLNIGMPVFFTETLLWMPFVYAPVIIAGYIVWRQTVVD
                     AGWPHWRNLVRFLNGHGLRENVQWKWEYVPFAIIFGYLGANMEHWVLGNEALVWDLGP
                     LDLSKLMLVMVVFVGFGEELVFRGLLQSSIATNFGRGVAVLASSLLFAAMHSGYHNVP
                     YLC"
     gene            936..1508
                     /locus_tag="PLJ11_06420"
     CDS             936..1508
                     /locus_tag="PLJ11_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOO04338.1"
                     /translation="MKEMGVEQLKVLSDPNRLAILSLLTIREMTTTAISNLLGLSVQN
                     AQYHMKKLLEAGLIVPTRTELVGNLVEKYYRSAFEPGIMGDAAGAGSVDIAERLNIVF
                     AALGAIKGILNRGIRLMDEQREYFMNVAERPNYPFGANYVILPVSAPAVEDAETLMSE
                     LNDRLKGLSDKHPEEGKPKFAVLYAVFPFE"
     gene            1557..1630
                     /locus_tag="PLJ11_06425"
     tRNA            1557..1630
                     /locus_tag="PLJ11_06425"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1592..1594,aa:His,seq:gtg)
     gene            complement(1612..2799)
                     /locus_tag="PLJ11_06430"
     CDS             complement(1612..2799)
                     /locus_tag="PLJ11_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF835 domain-containing protein"
                     /protein_id="HOO04339.1"
                     /translation="MLELGPLTPAFLFLAISEVALGTYVLSKGRTSPVNRTFFWLTIL
                     AGTGSLLDLLVASLTYAELAAWAFRSLVFLLVIEMGVAYHLSTLVPFPSPMLLLGNDR
                     RAYGALVLGTAIAFAAAVGPMVNDRYGWVPATDLPFAFLLLTVYLAALVLTLRRKWRT
                     LEGLNRGRTGILFLALAVPALVMIAIMGMATTGVEVPRMYGLGELVSVALLTYGILRY
                     QLLVIPRGQEAAAPKDRIPSLARGRAYLFEGKDSDRMFRAVVQEVSKGTAALVISRAH
                     PDHLRSRYMLAHTPFLWLAQSPGPDRIDPTNLQLLTHMVLEFIKEGPALIAIEGLEYI
                     IVNNDLTRVLKFLGQLRDSVMVSGSVLLVVVDPATLTERQRAILERELEVIKEEEGAE
                     TAI"
BASE COUNT          640 a          839 c          937 g          556 t
ORIGIN      
        1 gcagaggtag ggaacgttgt gatagccgga gtgcatggct gcgaaaagga gggaggaggc
       61 gagaacagcg acgcccctcc cgaagttggt cgctatggaa ctctgcaaca ggccgcggaa
      121 gacaagttcc tccccgaagc cgacgaaaac gaccattacc agcatgagct tgctcaggtc
      181 aaggggcccg aggtcccaga ccagggcctc gttgcccagc acccagtgct ccatgttggc
      241 gcccaggtac ccgaatatta tcgcgaaggg gacgtactcc catttccatt ggacattttc
      301 gcggaggcca tgcccgttca ggaagcgcac caggttgcgc caatgcggcc accccgcatc
      361 caccaccgtc tgcctccaga cgatgtagcc tgcgatgatc accggggcgt agacgaaggg
      421 catccaaagc aacgtttccg tgaagaacac cggcatgccg atgttcagga ccctgagcag
      481 ggagaccagg gagaaggatt gatagaggag catctgcttc tccgcccaga tcggaagcag
      541 gatgcacagg aagatgttga gggcgtgcac ctccaggcag gcttcggtcc ggccgttgaa
      601 gaacagcccc tccgctagca cgatcagcgc cgttggcagc agtagaccga ccttatcccg
      661 catcggcccg ttgaatgggg agggacgatt aattactatc gtctggaatg ggtaaaaaag
      721 gaataggagg ataggtcatc ggtagaaggc ctacggtctg aggaaaaaga tgttgccgtt
      781 cactatgaca aagttcggct ggcccaaggt cttcttcact tcattccctc ccctaattat
      841 ttcttaacgg tataatctga tttgagcgat gattaatata atatttaatc attttcaaca
      901 tactagtgcg ttgaaaaaag attttagtgg tcaaaatgaa agagatgggc gttgaacagc
      961 taaaggtgct ctcggacccc aatcgcctgg ccatactgtc cctgcttacg ataagggaga
     1021 tgacaacgac cgccatatcg aacttgctcg gcttgagcgt gcagaatgcc cagtatcaca
     1081 tgaagaaatt gctggaggcc ggcctgatcg tgccgacgcg cacggagctc gtcggcaacc
     1141 tggtggagaa gtactatcgc tcggcgttcg agccggggat aatgggggac gcggcggggg
     1201 cgggctcggt ggacatcgcc gagcggctca acatcgtctt cgcggcgctt ggggcgatca
     1261 aggggatctt gaaccgcggg atccggctta tggatgagca gcgggagtac ttcatgaacg
     1321 tggccgagcg gcccaactat ccgttcggag ccaactatgt gatcctgccg gtaagcgccc
     1381 ccgccgtcga ggacgccgaa acccttatgt ccgaactcaa cgaccgcctg aaggggctgt
     1441 cggacaagca tcccgaggag gggaagccca agttcgcggt cctgtacgcg gtctttccgt
     1501 tcgagtaaga gggcgaatat ttattaatct tgaagcgatg ctgagattcc atcatggccg
     1561 ggatggggta gcctggttat cctgtgggac tgtggatccc tagacccgcg ttcaaatcgc
     1621 ggtctcggcc ccttcttctt cctttatcac ctctagctcc cgttcgagga tggccctctg
     1681 gcgttcggtg agcgtggccg ggtccaccac gaccaggagg accgagccgc tcaccattac
     1741 gctgtcccgg agctgcccca ggaacttcag caccctggtc aggtcattgt tcacgattat
     1801 gtactcaagc ccctcgatgg cgatgagggc cggtccttcc ttgatgaact ccaggaccat
     1861 gtgggtcagg agctgcagat tggtggggtc gatccggtcc gggcccgggc tctgggccag
     1921 ccacaggaag ggggtgtgcg ccagcatgta gcgggagcgc agatggtcgg gatgggccct
     1981 gctgattacc agcgccgctg tccctttgct tacctcttgc accaccgccc tgaacatcct
     2041 gtccgagtcc ttgccctcaa agaggtacgc ccttcccctg gccaatgatg gtatgcggtc
     2101 cttgggagcg gcggcctcct ggcccctcgg gatgacgagc agttggtacc gaaggatccc
     2161 ataggtcagc agggcaacgg aaacgagctc cccgagcccg tacatgcgcg gcacctccac
     2221 ccccgtggtg gccatgccca tgatcgcgat catcaccagc gccgggacgg ccagggcaag
     2281 gaagaggatc cccgtccttc ccctgttcaa gccctccagg gtgcgccatt tccttcggag
     2341 ggtgaggacg agggccgcca gatagacggt gaggaggagg aatgcgaagg gcaggtcagt
     2401 ggcggggacc cagccgtagc ggtcgttgac catgggcccg acggcggcgg cgaaggcgat
     2461 cgcggtcccc agaacgagcg ccccataggc cctgcgatcg ttccccagca ggagcatggg
     2521 ggaggggaac ggcacaaggg tgctcaggtg ataggccacg cccatctcga tcaccagcag
     2581 gaataccaac gatcggaacg cccaggcggc cagttcggca taggtgaggc tggccaccag
     2641 gaggtccagc aggctgcccg ttccggccag aatggtcagc cagaagaagg tgcggttgac
     2701 gggggaggtg cgccccttgg acaggacgta tgtccctagg gcgacctcgg agatcgccag
     2761 gaataggaat gcgggcgtga gcggccctag ctccaacata gcagccccct tcatcataag
     2821 ctcggtggct aataatgatg cccatggacc ctggcagacc atcgcattgg aggcggggtt
     2881 tcctataaat cccggaagcg gcatgggatg ggcgtcgagg tagatggaaa tgggctggaa
     2941 tgggccgctc gagcagatcg atgcctatcg gt
//
LOCUS       DAOSYN010000168         2937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_26411_length_2937_cov_3.253990, whole genome shotgun sequence.
ACCESSION   DAOSYN010000168 DAOSYN010000000
VERSION     DAOSYN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26411_length_2937_cov_3.253990"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1181)
                     /locus_tag="PLJ11_06435"
     CDS             complement(297..1181)
                     /locus_tag="PLJ11_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOO04340.1"
                     /translation="MKENDELCRITAQDILKEQPEPENEMTFLAWLLTHKGMGQTWTR
                     MIDGFVCKLLRDSNNPFPTRKDIDDIAVKMWSSSYSKSHQRHLLMGFEYCCEWKGMKV
                     KFRKPRAKKRNPRYLTEAQMKHLCKASRDFREYALLKILCTTGLRLNELRMLNISDVD
                     LNLRRLTVRHAKRDKEREIPLSEDVCKLIATYISKYHNRPGLTEPLFISAKGNRWSAH
                     AIQANIKRCAERAKLKEKVTPHVLRHSFATAMLGNGCDLFHISHMLGHSDISTTTIYL
                     HVNDRAMRDTYNKGVPRL"
     gene            complement(1309..1392)
                     /locus_tag="PLJ11_06440"
     tRNA            complement(1309..1392)
                     /locus_tag="PLJ11_06440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1354..1356),aa:Leu,seq:cag)
     gene            complement(1430..1975)
                     /locus_tag="PLJ11_06445"
     CDS             complement(1430..1975)
                     /locus_tag="PLJ11_06445"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOO04341.1"
                     /translation="MLSGEKVDLVAAERSYVPMYVRWINDPEVTQWLKADPPFSAEME
                     QAWVDRLRREGKMTFTILTKEGRPIGNLGMEEVSWKYKRATIGIMIGEKEYWGKGYGS
                     DALTTLLQYCFEELGMRRVDLITDVDNHRALRAYRKCGFKEEGVLRQYRTKNGRAVDC
                     VMMAVLAMDWFVLHPPRGTRT"
     gene            complement(1969..2496)
                     /locus_tag="PLJ11_06450"
     CDS             complement(1969..2496)
                     /locus_tag="PLJ11_06450"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOO04342.1"
                     /translation="MLRGELVGLVPLQTTDELALYRWLNDPRVRAKAGRPEWRAAYSL
                     EHVQDIIQERLARPSNLDLVVVDLKGEGELGLVGLAQLMPMRDSAQLYLIWGEKEGDP
                     TLEALALAVVYAFNSQSLHRLWTRVPAVEMERIALFEQVGFKKEGVLREDHFNGGGWR
                     DSVLLSMLSAEARPC"
     gene            2594..>2937
                     /locus_tag="PLJ11_06455"
     CDS             2594..>2937
                     /locus_tag="PLJ11_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOO04343.1"
                     /translation="MDLNIVLLGPPGSGKGTQTERLAAEFGFTKISTGDLLREAVRNG
                     TELGTKAKGYMDAGKLVPDALVIGLIKEKLKGSKGGVILDGFPRSLEQAKALDEVLKV
                     GLAVDLKVDQEAL"
BASE COUNT          606 a          862 c          807 g          662 t
ORIGIN      
        1 gcgcctcgac ctcggagcat cgaccaaggt cgatgctgaa ggtgtcgagg cgcgattgac
       61 cgtcaaactt gaatatgtcg gacgttattt ctacgaccgt ggcaaccaat ccagtcacct
      121 tctttggaca gctggcgaat ggattggacc acacttaaat attttgtaat caatattggt
      181 aacaataaat tagaatctaa ttgtctgaag ttggcaaagt gtcaggctga attttgcaac
      241 aaggcctcta tcaataaaaa acgaggaata aaaaggtttg gaggacttgg ttgaaactac
      301 aaccgaggca cacccttgtt gtaagtgtct cgcatcgccc tatcgttgac gtgcaagtag
      361 attgtggtag tgctgatgtc ggagtgtcca agcatgtggg agatgtggaa gaggtcgcat
      421 ccgttgccga gcattgccgt ggcgaagctg tgcctaagaa catggggtgt taccttttcc
      481 ttgagtttcg cacgctccgc acaccgttta atgttggctt gaatagcatg agcagaccag
      541 cggtttcctt ttgccgatat gaatagtggt tcagtcagcc cgggccggtt atgatatttt
      601 gagatgtagg tggcaatgag cttgcacacg tcctctgata gcggaatctc cctctccttg
      661 tccctcttag cgtgacgcac agtcaaacgc ctgagattga ggtctacatc gctgatattg
      721 agcattctaa gctcattgag cctgagcccc gttgtgcaga gaatcttcaa cagggcgtat
      781 tccctgaagt ccctagaggc tttgcagaga tgtttcatct gggcctcagt cagataccta
      841 gggttacgct ttttcgcccg gggcttgcgg aactttacct tcatcccctt ccactcgcag
      901 cagtattcaa aacccatgag caggtgcctc tggtgggact tggagtagct ggatgaccac
      961 atctttacgg caatatcgtc aatgtccttc ctggtgggga aggggttgtt gctgtccctg
     1021 aggagcttgc acacgaagcc atcaatcatc ctagtccacg tctgacccat tcctttgtgc
     1081 gttaaaagcc acgcaaggaa tgtcatttcg ttctctggct cgggctgctc ttttagaatg
     1141 tcttgtgccg tgattcggca taattcgtcg ttttccttca tgctggctca cttcccgtcc
     1201 agcacgaact ggcttacaag cttggccaac agagggccgt tcaaggcctc gattcccgaa
     1261 gggaatatgg cggaagagct tagttagcca gaatagtaga atcaaaagtg caggggaagg
     1321 gatttgaacc ctcggaccac taaggactag accctgaatc tagcgccgtt ggccaggctt
     1381 ggctacccct gcctaggatt aagccgaagg atattttcgt actaatacct tatgtcctcg
     1441 tcccccgggg cggatgcagg acgaaccagt ccatggccag gaccgccatc atgacgcagt
     1501 cgaccgccct cccgttcttt gtgcggtatt gtcgcagcac cccctcctcc ttgaacccgc
     1561 atttccggta ggcccgcagg gcccgatggt tatccacgtc cgtgatgagg tccactctcc
     1621 tcatcccaag ctcctcgaag caatactgca gaagggtcgt cagcgcatcg ctcccatagc
     1681 ccttccccca gtactccttc tccccgatca tgatgcctat cgtcgcccgt ttgtacttcc
     1741 aggagacctc ctccatgccc aggttcccga tgggcctgcc ctccttggtc aggatggtga
     1801 acgtcatctt cccctcgcgg cgcaggcggt ccacccatgc ctgctccatc tccgcgctga
     1861 agggggggtc ggccttcagc cactgggtga cctcggggtc gttgatccac cgcacgtaca
     1921 tgggcacata gctccgctcg gccgccacca ggtccacctt ctccccgctc agcacggcct
     1981 cgcctccgcc gagagcatcg agagcagcac cgagtccctc cagccgcccc cgttgaaatg
     2041 gtcctcccgc agcacccctt ccttcttgaa accgacctgc tcgaaaaggg cgatcctctc
     2101 catctcgacc gcagggaccc ttgtccacag gcggtgcagg ctctgggagt tgaaggcgta
     2161 gacgaccgcc aatgcaagcg cctcgagcgt cgggtccccc tccttctccc cccaaatgag
     2221 atagagctgg gcggagtccc gcatcggcat cagctgggcg agccccacca gccccagctc
     2281 cccctcccct ttcaggtcga ccacgacaag gtcgaggttg gatggcctgg caagtctttc
     2341 ctgaatgata tcctgcacgt gctccaggct ataggcggcc ctccattcgg gccttccggc
     2401 cttcgccctc acccgggggt cgttcagcca tctgtacagc gcaagctcgt ccgtggtctg
     2461 caaggggacc aaccccacca gctcccctct caacatggag ctttgatcgc caggatggaa
     2521 gataatccta ttggcctaag cggatcggga cgggaatcgt aaagatgatt taggaagagc
     2581 ggcatggcgc accatggacc tgaacatcgt gcttttgggg cctccgggat cgggcaaggg
     2641 aacgcagacc gaaaggctgg cagctgagtt cggtttcacc aagatatcca ccggcgacct
     2701 gctgcgcgag gcggtccgca atgggacgga gctagggacg aaggccaagg ggtacatgga
     2761 cgccgggaag ctggtcccgg acgccctggt gatcggcctg atcaaggaga agctgaaggg
     2821 ctcgaagggc ggcgtgatcc tggacgggtt cccccgctcc ctcgagcagg cgaaggccct
     2881 ggacgaggtc cttaaggtgg ggctggccgt ggacctgaag gtcgaccagg aggcgct
//
LOCUS       DAOSYN010000169         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_26530_length_2924_cov_3.822586, whole genome shotgun sequence.
ACCESSION   DAOSYN010000169 DAOSYN010000000
VERSION     DAOSYN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26530_length_2924_cov_3.822586"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLJ11_06460"
     CDS             <1..709
                     /locus_tag="PLJ11_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="catalyzes condensation of pyruvate and acetyl-CoA
                     to form (R)-citramalate; functions in isoleucine
                     synthesis; belongs to the alpha-IPM synthetase/homocitrate
                     synthase family; it is difficult distinguishing these
                     proteins from enzymes in that family; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOO04344.1"
                     /translation="DLDYLLSVYMVAEAAGADRVGVTDTLGCASPEAIAFLVARAKEV
                     VRVPVSLHLHNDYGLALANAIAGVKAGADAVTTTVNGIGERCGNVPLEQFVAAMKFMY
                     GKDLGIDTTKLKELSDMVEAFSGLAKPVNQPLVGRNAFAHESGIHVAAVLACPLTYES
                     IDPRLVGNERHILMGKHSGLNYIKKRVDDIGLIATDEQLAEILGRVKDIGERKGRVND
                     LEFREIVASVIRPVLIT"
     gene            complement(699..1487)
                     /locus_tag="PLJ11_06465"
     CDS             complement(699..1487)
                     /locus_tag="PLJ11_06465"
                     /inference="COORDINATES: protein motif:HMM:NF000796.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOO04345.1"
                     /translation="MIDVRAASQAMVAIVSRNEEAKDVVTLRFDWDVPATPGQFVMVW
                     VPGVDEVPMSLSYLGARKGITVKGVGEATNALTAMQVGGMIGVRGPYGRGYRIPEGKV
                     LAVGGGTGMASLLPAVEAVPSAEVHTAIGARTEAELLFEERAKNASATVRVSTDDGSR
                     GLHGNAVQLAKAMMAETRYDVVLGCGPERMLYHLHRLCLEQGIPCQMSLERYMRCGAG
                     LCGSCALDGSRVCREGPVFTGEELKGMREFGKQRRDECGRRVRL"
     gene            complement(1480..2388)
                     /locus_tag="PLJ11_06470"
     CDS             complement(1480..2388)
                     /locus_tag="PLJ11_06470"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOO04346.1"
                     /translation="MTSLKVVLAGVEMENPTMLASGIMGETGGSLLAMAKGGAGALVT
                     KSIGSVPREGHKNPTLVELEFGYINAMGLPNPGIEAFRDEMFEAGKAGVPIVGSVFGA
                     SAEEFASLARRMQEYGAEAVELNLSCPHAKGYGMEVGTDPDVVASIIREVKEAVVVPV
                     FAKLTPNTHRLIDVAKAAEEAGADAIVAINTLRAMKIDVDARRPMLSNRYGGLSGPAV
                     RGVGVRCVYELFEALKVPVIGVGGIEDWRSALEYMMAGAAAVQIGSGVGRMGPQVFGD
                     ICSGISAYMARNGFQDISQVVGVAHD"
     gene            2670..2870
                     /locus_tag="PLJ11_06475"
     CDS             2670..2870
                     /locus_tag="PLJ11_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04347.1"
                     /translation="MLTEDGKAMLTRTVQEYLMEHPGNKKEAKRKAIRHFMDYRMAFG
                     GGKVSEKLMKEVEGYIDHVLSF"
BASE COUNT          500 a         1029 c          886 g          509 t
ORIGIN      
        1 ggacctggac tacctgttga gcgtgtacat ggtcgccgag gccgcggggg cggaccgcgt
       61 gggggtgacg gacaccctcg gctgcgcgtc ccccgaggcc atagcgttcc tggtcgcccg
      121 ggccaaggag gtggtgaggg tgcccgtgtc ccttcacctc cacaacgact acggcctcgc
      181 cctggccaac gccatcgccg gggtcaaggc cggggcggac gccgtgacga cgaccgtcaa
      241 cggcataggc gagcggtgcg ggaacgtccc gttggagcag ttcgtggcgg ccatgaagtt
      301 catgtacggc aaggacctgg gcatagacac gaccaagctg aaggaactct cggacatggt
      361 ggaggcgttc tccggcctgg cgaagccggt caaccagccc ctggtcgggc ggaacgcctt
      421 cgcccacgag tccgggatcc atgtggcggc ggtgctggcg tgcccgttga cgtacgagag
      481 catcgacccg aggctggtcg gcaacgagcg gcacatcctg atggggaagc attccggcct
      541 gaactacatc aagaagaggg tggacgacat cggcctgatc gccacggacg agcagctcgc
      601 cgagatactg ggaagggtta aggacatagg ggagaggaag ggcagggtga acgacctcga
      661 gttcagggag atcgtcgcct ccgtcatccg cccggtgctc ataacctgac ccttcggccg
      721 cactcgtccc ggcgctgttt cccgaactcc ctcatcccct tgagttcctc gccagtgaac
      781 accgggcctt cccggcatac gcggctgccg tcgagggcgc aggagccgca cagcccggcg
      841 ccgcacctca tgtagcgttc cagggacatc tggcatggga tcccttgctc caggcacagc
      901 cgatgcaggt ggtagagcat cctttcggga ccgcagccca ggacgacgtc atacctcgtc
      961 tcggccatca ttgccttcgc cagctgcacc gcgttcccgt ggaggcccct gcttccgtcg
     1021 tcggtcgaga cccgcacggt cgccgacgcg ttcttcgccc tctcctcgaa gagcagctcc
     1081 gcctcggtcc gggccccgat ggcggtgtgc acctccgccg atgggacagc ctccacggcc
     1141 ggcaggaggg acgccatgcc ggtgccccct cccaccgcca ggacctttcc ctcagggatg
     1201 cggtaacccc gcccgtaggg gccgcgcacg cctatcatcc caccgacctg catcgccgtg
     1261 agggcgttcg tcgcctcgcc cacgcccttc accgtgatgc ccttccgggc gcccagatag
     1321 gagagggaca tcgggacctc gtcgacgccg gggacccaga ccatcacgaa ctgaccgggg
     1381 gtggcgggca cgtcccaatc gaacctgagg gtcacgacgt ccttggcctc ctcgttcctg
     1441 gagactatgg ccaccatggc ctgggaggcc gcccggacgt caatcatggg ccacccccac
     1501 cacttgggat atgtcctgga acccgttccg cgccatgtag gcggatatcc cggagcagat
     1561 gtccccgaag acctgcgggc ccatcctgcc gacgccgctg cctatctgga ccgccgccgc
     1621 ccccgccatc atgtattcca gggccgagcg ccagtcctcg atgcccccga cgccgatgac
     1681 cgggaccttg agcgcctcga acagctcgta gacgcacctt acccccacgc ccctcacggc
     1741 cgggccggac agcccgccgt aacggttcga gagcatcggc cgcctggcgt ccacgtcgat
     1801 cttcatcgcc ctcagggtgt tgatcgccac tatggcgtcc gccccggcct cctccgcggc
     1861 cttggccacg tctatcagcc tatgggtgtt gggggtcagc ttggcgaaga ccgggaccac
     1921 caccgcttcc ttgacctccc tgattatgct ggcgacgacg tctgggtccg tcccgacctc
     1981 catgccgtac cccttggcat gcgggcagct caggttcagc tccacggcct ccgccccgta
     2041 ctcctgcatc ctcctcgcca gggaggcgaa ctcctccgcg gaggcgccga acacgctgcc
     2101 gacgatcggg acgccggcct tcccggcctc gaacatctca tccctgaacg cctcgatccc
     2161 cgggttgggc agccccatgg cgttgatgta gccgaactcg agctcaacga gggtgggatt
     2221 cttgtgcccc tcgcgcggaa cgctgcctat cgacttggtg accaaggccc ccgcgccgcc
     2281 cttggccatg gccagcaggg agcccccggt ctcgcccatt atgcccgagg ccagcatggt
     2341 ggggttctcc atctccaccc cggccaggac caccttcagg gatgtcatcg ctcagcgcat
     2401 gccgtccggg gatattaatc tcgctccgcc ccgccaccgg gatgccgtgg ctacgcgccc
     2461 ctcgatcggc gtggccgccg ccgtcaaagc catcaccagg tccgctctgg tcctacgtcc
     2521 ttcgagcccc tcggacctcg tcggtcctct tgcctgaagg cccgatgccc gctcgccccg
     2581 gtggcctttc gttttcttac ccattccgaa aatgatttcg caaaccttaa accgtcgttc
     2641 ggacatagta tcgaatgggg gttcaaacca tgttaaccga ggacggcaag gctatgctga
     2701 cccgaactgt ccaggagtac ctgatggagc atccaggcaa taagaaggaa gcgaagagga
     2761 aggctataag gcacttcatg gactaccgga tggcctttgg cggaggaaag gtctccgaga
     2821 agcttatgaa agaggtggag gggtacatcg accacgtact gtccttctga cctccttttt
     2881 ttacacaaag gcgttaatag gatccaatcc gttcccagcc ccga
//
LOCUS       DAOSYN010000170         2908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_26713_length_2908_cov_4.567473, whole genome shotgun sequence.
ACCESSION   DAOSYN010000170 DAOSYN010000000
VERSION     DAOSYN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26713_length_2908_cov_4.567473"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1643)
                     /locus_tag="PLJ11_06480"
     CDS             complement(186..1643)
                     /locus_tag="PLJ11_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04348.1"
                     /translation="MASLLSLMLKEEYRMHTRYSSRQLFLSLPLYIFILTALFTFGLD
                     ILTGDLRLWDLMLLAYGGMFIYGLSVGAFGVLGQTYLERRYGKDNFLIGMPFLLPVTF
                     RRAFLCMYLRDVIFYSVLMLLPALGGVALGAALSSHSVLSVLLVFLGLLVTFLYGMSV
                     SFLISVLYGIDRRLFLLAVGALLALIAGYGLTDLYGLELIIPSIGYQLHARPFQMDWG
                     RASGYLAACVALSISLLVLSLLLVRPERAAIAGTHREMFLQHLRRFSRFPSPTLLAKE
                     FVDIRRSGLTVRMLFAYVVPLVFLSFTTYYVNHGLSIPVGFNAVFYGAMVGFFGVLLY
                     SWMTNIDLNDYYETLPVRVPQVIRTKLAAFFLLTTGVSTLFVVAISLMNDEPQLLWLA
                     LIVLYCTSAYMVLATAYLTGLSTNSFFFNPTILTKFTAISVLPDLLLTVLSFSLQEGA
                     LWAFAGILGVCGALVLLSAYFYRGIERRWGNVGFL"
     gene            complement(1627..2355)
                     /locus_tag="PLJ11_06485"
     CDS             complement(1627..2355)
                     /locus_tag="PLJ11_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04349.1"
                     /translation="MPAIVVEGLTKRYGGFLALDSATFTVGEGTFFGCFGPNGAGKST
                     LLRLLTGQMPPTSGTAEVLGLDPAKDAVEVKRRVGIVPEVESPPSYLTVSEFLRFAGS
                     LHGVAQLDGRIDRWLSFFDLRGAEGMLCRDLSKGMRQKTMLAAAFLHEPRLLFLDEPF
                     INLDPIYQRKLRDYLLELREDGRTVFLCSHILDMAQRMVQEMIILDHGRVVLRDTVSN
                     ALARGEDLETLFLRLVGGGNGLAP"
     gene            2469..2801
                     /locus_tag="PLJ11_06490"
     CDS             2469..2801
                     /locus_tag="PLJ11_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04350.1"
                     /translation="MGHSVLTSKLEAEVELLERHVMMLDAIKRHEPIGIIRLSELLDI
                     PQHKVRYSLRILEQEGLINPSPEGAVTTERLDEFYERVVEILDRMEATVSKLKGELEE
                     ESKRNVKD"
     gene            2833..2905
                     /locus_tag="PLJ11_06495"
     tRNA            2833..2905
                     /locus_tag="PLJ11_06495"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2866..2868,aa:Asn,seq:gtt)
BASE COUNT          614 a          892 c          968 g          434 t
ORIGIN      
        1 ggagcgcctc gaatcccagg atgaggagcg tgagcaggac gagcgcctgc cccttccgca
       61 cgccggggag cagcgggtcc ggaaggaggt agtagacgca gagcaggggc gccagaaggt
      121 gcaccgatcg ccgcacccag tgccaatccg catgctcccc cttcccctcc ctcatcttcc
      181 ccgcctcaca ggaagccgac gttcccccac ctccgctcta tgccccggta gaagtatgcg
      241 ctgagcagga ccagggcccc gcacacccca aggatgccgg cgaaggccca cagggccccc
      301 tcctgcaggc tgaaggacag gaccgtcagc aacaggtccg ggagcacgga gatggcggtg
      361 aacttggtca ggatggtggg gttgaagaag aaggagttcg tgctcagccc cgtcaggtac
      421 gccgtggcca ggaccatgta ggccgacgtg cagtagagga cgatcagggc gagccagagc
      481 agctgcggct catcgttcat cagggagatg gcgacgacga agagcgtcga gacaccggtg
      541 gtcaggagga agaaggccgc cagcttcgtc ctgatgacct gcgggacccg cacgggcagc
      601 gtctcgtaat agtcgttcag gtcgatgttg gtcatccagc tgtagagaag gacgccgaag
      661 aagccgacca tcgcgccgta gaacacggcg ttgaagccca ccggtatgct cagcccatgg
      721 ttcacatagt aggtggtgaa gctcaggaac acgaggggga ccacgtaggc gaagagcatg
      781 cggacggtca gcccggagcg ccggatgtcc acgaactcct tggccaggag ggttggggag
      841 gggaacctgg agaagcgcct cagatgctgc aggaacatct cccggtgcgt gcctgcaatg
      901 gcggcccgct ccggccgcac caatagcaac gagaggacca ggagggagat gctgagggcg
      961 acgcaggccg cgaggtaccc gctcgccctc ccccagtcca tctggaaggg ccgggcgtgg
     1021 agctggtacc cgatgctggg gatgatcagc tccagcccgt agaggtccgt cagcccgtac
     1081 ccggcgatca gagcgagcaa cgcccccacg gccaggagga acagccgccg gtcgatgccg
     1141 tagagaacgg agatgaggaa gctgacggac atgccataga ggaacgtgac cagcagccca
     1201 aggaagacga ggaggaccga gaggacggaa tgagaggaga gggcggcgcc cagggccacg
     1261 cccccgaggg cgggcaggag catgaggacg gagtagaaga tgacgtccct gaggtacatg
     1321 cagaggaagg cgcggcggaa ggtgacgggg agcaggaacg gcatcccgat caggaaattg
     1381 tccttcccgt acctgcgttc caggtacgtc tgccccaata ccccgaacgc ccccacgctc
     1441 aggccgtaga tgaacatccc gccgtaggcc aggagcatca ggtcccagag ccggaggtcc
     1501 cctgtgagga tgtcgagccc gaaggtgaac agggcggtga ggatgaagat gtacagcggg
     1561 agggagagga agagctggcg cgaggagtac ctcgtgtgca tgcggtactc ctccttcagc
     1621 atgaggctaa ggagcgaggc cattcccccc gcccaccagc ctcaggaaga gcgtttccag
     1681 gtcctcgccc ctggccagcg cgttggagac ggtatccctc agcaccaccc gcccatggtc
     1741 caatatgatc atctcctgga ccatcctctg ggccatgtcg aggatgtgcg agcacaggaa
     1801 gaccgtccgc ccgtcctccc tcagctccag caggtagtcc ctcagcttgc gctggtagat
     1861 cggatcgagg ttgatgaagg gctcgtccag gaagagcagc ctcggctcgt ggaggaacgc
     1921 cgcggccagc atcgtcttct gcctcatccc cttggacagg tcccggcaca gcatcccctc
     1981 cgcgcccctc aggtcgaaga agctcagcca gcggtcgatg cggccgtcga gctgcgccac
     2041 gccgtgcagg ctccccgcga acctcaggaa ctcggagacg gtgagatatg agggcgggct
     2101 ctccacctcc gggactatgc cgacgcgcct cttgacctcc actgcgtcct tggcagggtc
     2161 cagccccagg acctcggctg tgccgctggt cggcggcatc tgccccgtga ggagcctgag
     2221 cagggtggac ttcccggccc cattgggccc gaagcacccg aagaacgtgc cctcgcccac
     2281 cgtgaaggtg gcggagtcca aggccaggaa gcccccgtac ctcttggtca ggccttcgac
     2341 gacgatcgcg ggcatgcggg aatcatcaag cgcgatgtat ataaggcgtg cgcgctcggg
     2401 aagggcgaaa tggatttaac cccttcaaat gctatcacgt gtctagcgcc ggggatgtag
     2461 ccgccatgat gggtcatagc gttcttacca gcaaacttga ggccgaggtt gagctcctgg
     2521 agaggcacgt gatgatgctg gacgcgatca agaggcatga gcccatcggc atcatccggc
     2581 tctcagagct cctagacatc ccgcagcaca aggtccgcta ctccctgcgc atcctggagc
     2641 aggaggggct gatcaatccc tcgcccgagg gcgcggtcac caccgagcgg ctcgacgagt
     2701 tctacgaacg ggtggtggag atcctggacc ggatggaggc caccgtcagc aagctcaagg
     2761 gggagctgga agaggagtcc aagaggaacg taaaggatta atattggacc cctgttctac
     2821 agcccacatc gtgccgtcgt agctcagtcg gtagagcgtg tgactgttaa tcacaaggtc
     2881 agcggttcga tcccgctcga cggcgcct
//
LOCUS       DAOSYN010000171         2905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_26748_length_2905_cov_3.835439, whole genome shotgun sequence.
ACCESSION   DAOSYN010000171 DAOSYN010000000
VERSION     DAOSYN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26748_length_2905_cov_3.835439"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLJ11_06500"
     CDS             complement(<1..310)
                     /locus_tag="PLJ11_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04351.1"
                     /translation="MSEFARGSLRTFWSSAVLAKRALFAWLNPVMWLMQLFGMSLFQI
                     AFFVYVAMFVNNAEVTIEYVAIGNALQSMAYVSVFAVCNITGEEKNQGTIEGLLVSPA
                     N"
     gene            complement(312..1100)
                     /locus_tag="PLJ11_06505"
     CDS             complement(312..1100)
                     /locus_tag="PLJ11_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO04352.1"
                     /translation="MPRLRAATASFRHQASMFLAEPQWLIPNIIAPFVLAMVVLMLYR
                     EPSPEAVLYAVLGGGMMGMWGNTLYASGYSIQSDRWWGTVDAIFSAPSPLIWIIGGRT
                     VWNAFIGIVNGLFVLVAAMLIFRTPLEVANFPMFLLAFVLTLLSLAALGMLFSAAFVL
                     TRSAQVLTNGLEFPIYVGTGSMFPIFLLPYWTHPLSLSLAPTWGIDAIRYSALAGYAQ
                     GFRIGYWGDMLAMAALSLLYLVISIWLFKAVDRRARRTADLMRY"
     gene            complement(1093..2076)
                     /locus_tag="PLJ11_06510"
     CDS             complement(1093..2076)
                     /locus_tag="PLJ11_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04353.1"
                     /translation="MDDREIVIDVRGLVRKYHSTTGVLHQKRRETVAVDHIDLQVRRG
                     ELFGLLGPNGAGKTTTIKVLTTLLLPTEGSVQVLGYDAVKQPMEVRKRIGLILGGERG
                     LYYRITARQNLRYFADIYGVPIAIRDKRIEEVLARVDLLDKADVRVEDYSRGMKQRLH
                     IAKGIVHDPELVFMDEPTIGLDPEAARDTRAMTKELVGEGKTILLTTHYMYEADELCD
                     RVGVIARGRILALDTPSGLKALVKDTSVIEVEGFGLTEMDVESLRALEGVRTVAANMG
                     EDKQTMRVQVEDPGGMLPRVAHILEDRKVIGMKVKEPTLEDAYLWLVREDA"
     gene            2241..2642
                     /locus_tag="PLJ11_06515"
     CDS             2241..2642
                     /locus_tag="PLJ11_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO04354.1"
                     /translation="MAAKRRPAAEGERPCRMAVTSKGDDLDGEIAPMARSPFFIIFEG
                     TPENHLVMENLIRKGGSESALRTAAELVKQRVDIVITGTIGPRAYRQLEEAGIKVHAG
                     CEGTVSEALEKCLKGKLPICDKATYGGYLGI"
     gene            complement(2655..>2905)
                     /locus_tag="PLJ11_06520"
     CDS             complement(2655..>2905)
                     /locus_tag="PLJ11_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04355.1"
                     /translation="PQEGISFLLIAGLGVGGFRIPAALVIAIAAVMMMLGWNVWRRAR
                     IARSEREMRERDRLLCDYCGGQNAEGAVQCQFCGAPLR"
BASE COUNT          579 a          950 c          880 g          496 t
ORIGIN      
        1 ggttggccgg ggacaccaga aggccctcta tggtcccctg gttcttctcc tccccggtga
       61 tgttgcacac cgcgaagacg ctcacatagg ccatcgattg caaggcgttc ccgatggcca
      121 cgtactcgat ggtcacctcg gcgttgttta cgaacatcgc cacgtacacg aagaacgcga
      181 tctggaagag ggacatgccg aagagctgca tcagccacat gaccgggttg agccatgcga
      241 acagcgcccg cttggcaagc accgccgaac tccagaaggt gcgcagtgag ccccgtgcga
      301 attcggacat gtcagtacct catcagatcg gccgtcctcc gggcccgtcg gtccaccgcc
      361 ttgaacagcc aaatggagat caccagataa agcaacgaga gcgccgccat ggcgagcatg
      421 tccccccagt agccgatcct gaatccctgc gcgtacccgg ccagcgccga gtaccggatc
      481 gcgtctatgc cccatgtcgg ggccagggag agggacaggg ggtgggtcca atacggaagc
      541 aggaatatcg ggaacatgct ccccgtcccc acgtatatcg ggaactccag gccgttggtg
      601 agcacctgcg cggaccgggt gagcacgaag gcggcgctga acagcatgcc cagggcggcc
      661 agggagagca atgtgagcac aaaggccaat aggaacatgg ggaagttggc cacctccagc
      721 ggggtccgga agatgagcat ggccgccact agaacgaaca gcccgttcac gatgccgatg
      781 aacgcgttcc acaccgtccg tccgccaatg atccagatca gggggctggg ggcggagaag
      841 atggcgtcca ccgtgcccca ccatcggtcg gactggatgg agtaccccga ggcgtagagg
      901 gtgttccccc acatgcccat catccccccg cctaggaccg catagaggac ggcctccggg
      961 ctcggctcgc gatagagcat gagcaccacc atggcaagca cgaacggggc gatgatgttg
     1021 gggatgagcc attgcggctc ggccaggaac atcgacgcct gatgccggaa cgaggcggtg
     1081 gccgcgcgga gcctaggcat cctctctcac cagccagagg taggcgtcct ccaatgtcgg
     1141 ctccttcacc ttcatcccga tgaccttgcg gtcctcaagg atatgggcga ccctcgggag
     1201 catgcccccc gggtcctcta cctgcacccg catggtctgc ttgtcctcgc ccatgttcgc
     1261 ggcgaccgtc ctcacgccct ctagggccct caacgattcc acgtccatct cggtcaggcc
     1321 gaacccctcc acctcgatga ctgaggtgtc cttgaccagc gccttcagcc cgctcggggt
     1381 gtccagggca aggatcctac ctctggcgat cactccgacc cggtcgcaga gctcatccgc
     1441 ctcgtacatg taatgggtgg tcaggaggat ggtcttcccc tctcccacca gctccttggt
     1501 cattgcccgc gtgtccctcg ccgcctccgg gtccagcccg atggtgggct cgtccatgaa
     1561 gaccagttcc gggtcatgga cgataccctt ggcgatgtgc agccgctgct tcatgccccg
     1621 ggagtagtcc tccaccctga cgtccgcctt gtccaggagg tctaccctgg ccagcacctc
     1681 ctcgatccgt ttgtccctga tggcgatagg cacgccatag atatcggcga agtaacgcag
     1741 attctgccgg gcggtgatcc ggtaatacag cccgcgctcc cctcctagga tcaggccgat
     1801 gcgcttgcgg acctccatgg gctgcttaac cgcatcatac cccaggacct ggacgctgcc
     1861 ctcggtgggc agcaggaggg tcgtcaggac cttgatcgtg gtcgtcttgc cagcgccgtt
     1921 ggggcccagc agcccgaaga gctcgccccg gcggacctgc aggtctatat ggtccacggc
     1981 caccgtctcc ctccgtttct gatgaaggac cccggtcgtg ctatgatact tcctcaccaa
     2041 cccccgcacg tcgatgacga tctcccgatc atccattcct ctctcccccg tgctggggat
     2101 cccattggac gtaataacct ttcccgtgag cgcccgaaag tggatggatg tcattgaagg
     2161 cctaggacct tcaggggtaa ggaacgctag ttcgaaagaa ccttatgggc gcaagagcag
     2221 atagcatccg gtggtaaaaa atggcagcca agagaaggcc cgccgcggag ggcgaaaggc
     2281 cttgcaggat ggcggtgacg tccaagggcg atgacctcga cggggagatc gccccgatgg
     2341 cgagaagccc cttcttcatc atcttcgagg gcacccccga gaaccatctg gtgatggaga
     2401 acctcatcag gaagggaggg agcgagtccg cgctcaggac cgccgccgaa ctggtcaagc
     2461 agcgggtgga catcgtgatc accggaacga taggcccgcg agcgtatagg cagctggagg
     2521 aggccgggat caaggtgcac gccgggtgcg agggcacggt ctccgaggca ttggagaagt
     2581 gcctgaaggg caagcttccc atctgcgata aggcgacata cggcgggtat ctggggatct
     2641 aggccaacgg ccgttcaacg gaggggcgcc ccgcagaact ggcattgaac ggcgccctcc
     2701 gcgttctgcc cgccgcaata atcgcagagc aaccggtctc gctccctcat ctcccgctcc
     2761 gaacgggcga tgcgggccct cctccaaaca ttccacccaa gcatcatcat cacagcagcg
     2821 atggcgatca ctagcgccgc tggtatgcgg aaccctccga ccccgaggcc cgcgatgagc
     2881 aggaaggaga tgccctcctg cggac
//
LOCUS       DAOSYN010000172         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_27273_length_2858_cov_4.383161, whole genome shotgun sequence.
ACCESSION   DAOSYN010000172 DAOSYN010000000
VERSION     DAOSYN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27273_length_2858_cov_4.383161"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..887
                     /gene="rnhB"
                     /locus_tag="PLJ11_06525"
     CDS             246..887
                     /gene="rnhB"
                     /locus_tag="PLJ11_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOO04356.1"
                     /translation="MICGLDEAGRGPVMGPLVVAAVMVEDDGPLREWGVKDSKLLGPE
                     ERERLSHLIMANSSWKVVVIEPGTIDGRKGSTSLNTLEIRAFASLLDELRPQRAYVDA
                     CDVNANHFKAALTRAMKGRSELVCEHKADVNYPVVSAASILAKVRRDEEMRRIAEELG
                     DVGSGYSSDPKTRAFLENWIRENGDLPPHTRRSWATAKDLLGSSKISKLSDWE"
     gene            891..1223
                     /locus_tag="PLJ11_06530"
     CDS             891..1223
                     /locus_tag="PLJ11_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOO04357.1"
                     /translation="MTTKEHLLNAIVKFNDQMQSDAQLREEIGSLRKCVNIDLGTEHY
                     SFVLEDGHISQLEEGLLESPDIVVRSDPRTIDDLFTGKMKPMKAWALRKVVIKGSLED
                     VMKLRKFF"
     gene            1286..1360
                     /locus_tag="PLJ11_06535"
     tRNA            1286..1360
                     /locus_tag="PLJ11_06535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1321..1323,aa:Met,seq:cat)
     gene            1413..>2858
                     /locus_tag="PLJ11_06540"
     CDS             1413..>2858
                     /locus_tag="PLJ11_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO04358.1"
                     /translation="MRKDVLIIGGGATGAGIVRDLALRGVECALLEMGDYCHGASGGN
                     HGLLHSGGRYAVTDPPSATECAKENAILRRTAPFCIEGTDGLFVSLPGDDPSFADPFL
                     KGCKGSGVPAEELTAKEALSAEPGLSAEALRCIAVQDGSIDPFALVQANVDSAREAGA
                     MVRNHCRVTDMVLEGGMVVRVKALDRRSGEILTIVPEIVINAAGAWAPQVAAMAGCEV
                     AMGLDQGSMLVVDGRLCGHVVNRLRPPSDGDITVPNHTATVLGTTSRPAASPEDVAPS
                     RNDMELLLRETSAMLPALRHCRVVRAYGGVRPLPGRGGRGASRTFSLITDERAGNLLS
                     VVGGKLTTYRLMAERVSDQVCNALGVRAGCRTRTEPLGPPADEAGGSVALARLRRKYG
                     FKATTVSLAVQRMLCSCEQVLRAEAQMVLAGPDALALSDVMRRTRAGMGYCQGLDCAL
                     SLQELMMEAGGTEPLASLTEFLEERERGQALA"
BASE COUNT          541 a          867 c          949 g          501 t
ORIGIN      
        1 tgttccggcc gtcacgatgc acctgcaccg gcttcttgac cccggtcacc cccacacggg
       61 tcagcttgaa cccgttggta aggtaccggt tctgaacatc gctcttggaa attcacatca
      121 ccgcccctcc aacggggaaa cgactttata gcttagccca tcaacagctt ttttggtcaa
      181 actgcatgat attggtggat ggcgctctca acaacgcttt atacgcatta taaaatccca
      241 taccgatgat atgcggcctt gacgaggcgg gcagagggcc ggttatgggc cccttggtcg
      301 tagccgcggt gatggtggag gacgacggcc ccctccggga atggggggtg aaggactcca
      361 agcttctcgg tccagaggag agggagcgct tgtcccacct gatcatggcg aattcctcct
      421 ggaaggtggt ggtcatcgag ccggggacga tcgacgggcg caaggggtcc acttccctga
      481 acaccctgga gatcagggcc ttcgcctccc tgctggacga gctcagacct cagagggcgt
      541 acgtagacgc atgcgacgtc aatgcgaacc atttcaaggc cgcgctcaca agggcgatga
      601 agggcagatc ggagcttgta tgcgagcaca aggccgacgt gaactatccg gtcgtgtccg
      661 ccgcctcgat cctggccaag gtgcgccggg atgaggagat gcggcgcata gcggaggagc
      721 tgggggatgt tggcagcggc tactcctccg atcccaagac cagggccttc ctggaaaatt
      781 ggataaggga gaatggggat ctgccccctc atacccggag gtcatgggcg accgccaagg
      841 acctgttggg ttcgtccaag atttctaagc ttagcgactg ggagtgaccc atgacgacca
      901 aggagcattt attgaacgcc atcgtcaagt tcaatgatca gatgcagagc gacgcccagc
      961 tccgggagga gatcggatcc ctaaggaaat gcgtgaacat cgacctcggg acggagcact
     1021 actccttcgt gctcgaggac ggtcacatct cgcagctcga ggaaggattg ctcgaatccc
     1081 cggacatagt ggtgcgctcg gacccccgta ccatcgacga cctgttcact ggcaagatga
     1141 agccgatgaa ggcctgggcg ttgcgcaagg tcgtcataaa gggctccctc gaggatgtca
     1201 tgaagttgcg caagttcttc tgaaccagcc tgccccggag ggcagaaagg tttataaccg
     1261 gcctatgcat gatggggaag caccaagcgg ggtagggtag ccaggacatc ccgtcgggct
     1321 cataacccgg agaccggtcg ttcaaatcga ccccccgcta cctttctatc aatctccata
     1381 tatagactcg gtcattccct gtcccggctc acatgaggaa ggacgttctg atcatcggcg
     1441 gaggcgccac aggggcgggg atcgtcaggg acctcgccct gcgcggggtc gaatgcgccc
     1501 tgctggagat gggcgactat tgtcacgggg ccagcggggg caaccacggg ctcctgcaca
     1561 gcggagggcg ctatgccgtg accgatccgc cctccgcgac ggaatgcgcc aaggagaacg
     1621 ccatcctcag gaggaccgcg ccgttctgca tcgaggggac tgacgggctg ttcgtctccc
     1681 tcccagggga cgacccctcg ttcgctgacc cctttctaaa gggctgcaag ggcagtgggg
     1741 tccccgccga ggaactcacg gccaaggagg ccctttcggc cgagccgggg ctgagcgcgg
     1801 aagcgctcag gtgcattgcc gttcaggacg ggtccatcga ccccttcgcg ctcgtccagg
     1861 cgaacgtgga cagtgcccgg gaggccgggg ccatggtgcg caaccactgt cgtgtgaccg
     1921 acatggtgtt ggagggaggg atggtcgtcc gcgtcaaggc ccttgacagg cgcagcgggg
     1981 agatcctcac catcgtgccg gagatcgtca tcaatgccgc cggggcctgg gccccacagg
     2041 tggcggccat ggccggctgc gaggtggcca tgggactgga ccagggaagc atgctggtgg
     2101 tggacgggag gctctgcggc cacgtggtca accgtctgcg ccccccctcc gacggggaca
     2161 tcaccgtccc taaccacacc gccacggtgc tgggcaccac ctcccggccc gctgcgtcgc
     2221 cggaggacgt cgccccctcc cggaatgaca tggagctcct cctgcgagag acgtccgcaa
     2281 tgctgcccgc gctccgtcat tgccgcgtgg tccgggcgta tggaggggtc aggcccttgc
     2341 cgggcagggg tgggagaggg gcgagccgta ccttctccct gatcacggac gagagagcgg
     2401 gtaatctgct cagcgtcgtt gggggaaagc tgaccaccta tcggctgatg gcggagaggg
     2461 tctccgacca ggtctgcaat gcccttggcg tgcgggctgg atgtcgcact cgcacagagc
     2521 cgttggggcc cccggcggac gaggccggag gctcggtggc cctggcccgc ctgcggcgca
     2581 agtacggttt caaggcaacc acggtctccc tcgccgtcca gcggatgctg tgctcatgtg
     2641 aacaggtgct ccgggccgag gcccagatgg tcctggccgg gccggacgcc ctcgccctct
     2701 cggacgtcat gcgcaggacc agggcgggca tgggctattg ccaggggctc gattgcgcgc
     2761 tctccctcca agagctgatg atggaagccg gagggacgga gccattggcc tccctgaccg
     2821 agttcctgga agaacgggaa agggggcaag cattggcc
//
LOCUS       DAOSYN010000173         2837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_27542_length_2837_cov_3.475917, whole genome shotgun sequence.
ACCESSION   DAOSYN010000173 DAOSYN010000000
VERSION     DAOSYN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27542_length_2837_cov_3.475917"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLJ11_06545"
     CDS             <1..723
                     /locus_tag="PLJ11_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOO04359.1"
                     /translation="SPIALPELGFLELLVLPQDDMAEVKGFCDGFDGELIVHAPEELP
                     DGRPLDLASPDKGWREASVRRVREVTKAAEGTARPVVIHPGGATEAPVQEAGPLLGAL
                     RSSLEDLPDYTWLENMPLYYHHRGRRLHCNLMRTPGEMAEVEGLVAGFAVDVAHAYLG
                     VEGDGNANVRAMFVELGEAVRHVHLSDARRPDEKGLMPGDGEVDMSFLPYLRGLPVLL
                     EVGTGRLADGAALREALRRMRS"
     gene            798..1871
                     /locus_tag="PLJ11_06550"
     CDS             798..1871
                     /locus_tag="PLJ11_06550"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HOO04360.1"
                     /translation="MLGLDDGDFTKARSMFFPRQVLAGHDVIGTVPTSCKEFGLSGTA
                     LIVSGDKTMKVAGNVVRDGLRDSGYEVQVVNVGEATEENLAKVMEAARECRADFLLGV
                     GGGSKIDLAKMAATKLQKEFLSVPTSASHDGIASGRASIRSGAGPISHEAKVPLAVIA
                     DTAIIAKSPYRLLAAGCADVISNATALMDWEFARRLRSEEFSRSAYALANYTAQTIID
                     NADLIRPNLEESVWIAIRPIIISGISMSVAGSSRPTSGSEHMFSHALDIIAPGKALHG
                     EQCGVGCIMMMYLHGGDWNRIREALMKIGAPTSAKELGVTTEQVIQALVTAHQIRKDR
                     FTILGMGLTREAAERLATITRVI"
     gene            1890..2309
                     /locus_tag="PLJ11_06555"
     CDS             1890..2309
                     /locus_tag="PLJ11_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HOO04361.1"
                     /translation="MITVIGERQAKVDGQFIYLGPLTECKECKLKGVCFNLDAGALYR
                     IIEVRDVKHECKVHEDGVRVVKVEKERREGVAPKKGVVEGTTITYEPVKCDNLGCAYF
                     RLCHPSGMDRGRKERILKILGDVECAEGKRLVRVVFE"
     gene            2503..>2837
                     /locus_tag="PLJ11_06560"
     CDS             2503..>2837
                     /locus_tag="PLJ11_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04362.1"
                     /translation="MHIRWDDRSIFKAYMVAVVVLFVLYIIPVILFSFFGLDVPAVFG
                     PVRELGGAVYRGFFLVGIPLVSLFLGALSLGMLNGKERGPRSLPRSLGYLMLGVTLLS
                     LMIWIIDMSV"
BASE COUNT          499 a          839 c         1017 g          482 t
ORIGIN      
        1 tcccccatcg ccctgcccga actcggcttc ctggaattgc tggtgctgcc ccaggacgac
       61 atggcggagg taaaggggtt ctgcgacggg ttcgacggcg aactcatcgt ccacgcccca
      121 gaggagctgc cggacggccg gcccctcgat ctcgcctccc cggacaaggg atggcgggag
      181 gccagcgtcc ggagggtccg ggaggtcacc aaggccgcgg agggcaccgc ccgccccgtg
      241 gtcatccatc ccggcggggc gaccgaggcg cctgtccagg aggctgggcc gctgctgggg
      301 gcgctccgct cctccctgga ggaccttccg gactacacct ggctggagaa catgccccta
      361 tactatcatc accggggtag gaggctccac tgcaacctga tgcgcacccc gggggagatg
      421 gcggaggtgg aggggctggt cgcgggcttc gccgtggacg tcgcccatgc ctaccttggg
      481 gtggaggggg acgggaacgc caacgtccgg gcgatgttcg tggagttggg ggaggcggtg
      541 cgccatgtgc acctttcgga cgcccggagg ccggacgaga aggggctgat gcccggggac
      601 ggggaggtgg acatgtcctt cctcccgtat ctgaggggat tgcccgtgct cctcgaggtc
      661 gggaccgggc gccttgcgga cggagcggcg ctcagggagg cgctgcggcg gatgcgctcc
      721 tgatggccag catcctatgg cagggccaaa aaaccgataa cttgaagatt agatacctag
      781 ataggaggag gcattaggtg ctcggattgg acgatggaga cttcaccaaa gcgcgctcga
      841 tgttctttcc caggcaggtt ctggctggtc atgacgttat agggaccgtg cccacctcct
      901 gcaaggagtt cgggctcagc ggcaccgccc tcatcgtttc cggggacaag accatgaagg
      961 tggccgggaa cgtcgtccgg gacgggctga gggacagcgg ctacgaggtg caggtcgtga
     1021 acgtgggcga ggcgaccgag gagaacctgg ccaaggtgat ggaggccgcc agggaatgca
     1081 gggccgactt tctgcttggg gtcggtggtg gctccaagat cgacctggcc aagatggccg
     1141 ccaccaagct ccagaaggag ttcctctccg tgcccacctc cgcctcccac gacggcatcg
     1201 cgtccgggag ggcgagcatc cgcagcgggg ccggcccgat ctcccatgag gcgaaggttc
     1261 cgctggcggt gatcgccgac accgccatca tcgccaagtc accgtatcgc ttactggccg
     1321 cggggtgcgc ggacgtcatc agcaacgcca ccgccctgat ggactgggag ttcgcccgga
     1381 ggctgcgctc cgaggagttc tcccggtcgg cgtacgcgct tgccaactac accgcgcaga
     1441 cgataatcga caacgcggac ctcatccggc ccaacctgga ggagagcgtg tggatagcca
     1501 tccgcccgat catcatctcc gggatcagca tgagcgtggc cggatcgtcc cggcccacgt
     1561 cggggagcga gcacatgttc tcgcacgccc tggacataat agccccgggc aaggcgctgc
     1621 acggggagca gtgcggggtc ggctgcatca tgatgatgta ccttcacgga ggcgactgga
     1681 accgcatccg ggaggcgttg atgaagatag gggcccccac ctcggccaag gagctggggg
     1741 tcaccaccga gcaggtgatc caggcgctgg tgaccgccca ccagatccgg aaggaccgct
     1801 tcaccatcct gggcatgggg ctcacgaggg aggcggccga gcggctggcc acgatcaccc
     1861 gggtcatctg aggaagggag gctctacgca tgatcactgt gataggggag aggcaggcca
     1921 aggtagacgg ccaattcatc tacctggggc cgttgacgga gtgcaaggag tgcaagctca
     1981 agggggtgtg cttcaatctg gacgccgggg cgctgtaccg catcatcgag gttcgggacg
     2041 tcaagcacga atgcaaggtg catgaggacg gcgtgcgcgt cgtcaaggtg gagaaggagc
     2101 ggagggaggg cgtcgcgccc aagaagggcg tcgtggaggg gaccacgata acctatgagc
     2161 cggtcaagtg cgacaacctg ggatgcgcgt acttccggct ctgccatcca tcggggatgg
     2221 acagggggcg caaggaacgg atcctcaaga tcctcgggga tgtcgagtgc gccgagggga
     2281 agaggcttgt gagggtggtc ttcgagtagc cgtgcccggt cgggccggaa ggccccgggg
     2341 gtttcctcca cccatgcggg ccggagcatc cccggaacgc ggtttccctt ccttggggag
     2401 aaaggggccg cgggaagggc gtcctgggca tgataggttg aatagggttg aggaccatca
     2461 tacgaaccga aggatgcgct agaggccgtc aggtgatatg ggatgcacat cagatgggat
     2521 gatcgctcga tcttcaaggc ctacatggtc gccgttgtgg tgctcttcgt gctgtacatc
     2581 atccccgtca tcctgttctc cttcttcggg ctggacgttc ccgcggtctt cggccccgtc
     2641 agggagctgg gcggggcagt gtaccggggc ttcttccttg tcggcatccc tctggtgtcc
     2701 ctgttcctcg gggcgctgtc gctcggcatg ctgaatggga aggagagggg gccgcgctcc
     2761 ctcccccgct ccctggggta cctgatgctg ggcgtcaccc tgctgtcgct gatgatctgg
     2821 ataatcgaca tgtcggt
//
LOCUS       DAOSYN010000174         2813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_27877_length_2813_cov_3.743292, whole genome shotgun sequence.
ACCESSION   DAOSYN010000174 DAOSYN010000000
VERSION     DAOSYN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27877_length_2813_cov_3.743292"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..789)
                     /locus_tag="PLJ11_06565"
     CDS             complement(370..789)
                     /locus_tag="PLJ11_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04363.1"
                     /translation="MHTFIDADYGPHASDELVKRGPTTTSGTTTATVNIGVTLNQQGA
                     GVTCGYGWSYTISDVAVEETGDLSLETGNWKHEIENGCAASRCTLDVYPGASIRVNQG
                     GSIQPYESYANAFGYHGLWGWYVKSEQTYIHIPFILA"
     gene            complement(961..1620)
                     /locus_tag="PLJ11_06570"
     CDS             complement(961..1620)
                     /locus_tag="PLJ11_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04364.1"
                     /translation="MNKLCNLIVALLIVTLMVTTNVIALSVSPNVQNEENDDEINIIS
                     NIPLIILGSGNYYDIIINKMIEVSSDITLTSLDDLPMKVLSNEAVILVDATWVKTQDS
                     SEVSNALKVLLDKGAPLLCIGEMSDIIKQTGYSMDYVGGCDLIVQGIKVFDGGQTAVY
                     TGAGNIDDVDEMGEALSAAYSWALKWISNTIPDCRVSSSTGPLSNPCLLSGAYWGWVH
                     D"
     gene            complement(1985..>2813)
                     /locus_tag="PLJ11_06575"
     CDS             complement(1985..>2813)
                     /locus_tag="PLJ11_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04365.1"
                     /translation="RAGRQPLLHAEDVVRYGANGWAVNEDRLTSLFRAVKAVEGVERI
                     SISHFALASVASCPDAIREISGTMGLSEENWLGAQTGVETGSPSLMQHYMKGKCKPFS
                     PEEWPDVVVSGFRVCQENNWVPCGTIIMGLPGETEEDVLATIDLVHRLREYRSLIVPL
                     FFVATAEFVNGERSFEAKDMTPAHTRLFLDCWEHNLDWARRILGQYGGRSLRNPLTRV
                     AINVILRFGIVEASEVIRMCRKEYDYDIQRMMADGRDGKIRMFPLTVKAFRNMRPTA"
BASE COUNT          721 a          732 c          594 g          766 t
ORIGIN      
        1 gatgtatcaa taacggcatg atggaaacgt ggggaaccct tttaacaaag gtgagttcga
       61 cagtcggcaa gattattcaa gaaaaatgaa tggtcaatta atgctggata ccgtagcgtt
      121 cagcatcgaa gcattattga actcatctca atttcgacca aatcaactgt cgaagttagg
      181 ttaaatgttc ctaaaaatga cgttcactag gtcctagatg accaggctat tgggaggaat
      241 tagtattgat agtaaggttc gtaattataa tatcaagagg ttgataagat gctcaaggtt
      301 gaattatgta aaaaggaccg gtaaaaaaga tcatatcccc cgaattagag gggggatgaa
      361 ttaaaatggt caagccaata taaaaggtat atggatgtag gtttgttcac tcttcacata
      421 ccagccccat aatccatggt aaccaaatgc attggcataa gattcatatg gttgaatgga
      481 ccctccctga ttaactctta ttgaagcccc tggatagaca tctaacgtac atcttgacgc
      541 ggcacaaccg ttttctatct cgtgtttcca gtttcctgtt tctaaactta gatctcctgt
      601 ttcctcgacg gccacatcac tgattgtata tgaccagcca tatccacaag tgactcctgc
      661 cccttgttga ttcagagtta ctccaatatt aacagtcgcc gttgtcgttc ccgatgttgt
      721 tgtcgggccc ctttttacca actcatcgga agcatgaggg ccataatcag catctatgaa
      781 cgtgtgcatg tgcattgtat agtatcctct atcataagcg accttgcctg gtactgcttc
      841 ctgacaaaag tgtatagagt accaatcgta tacatctgta ttatcattat ataaatatac
      901 atattcattt tgtatgtttg ttcttccata cggttttagt gaatcgagag aattaaattc
      961 tcaatcatgt acccatcccc aatacgcccc agataacaag caaggattcg ataaaggtcc
     1021 tgttgaacta gatactctac agtcaggtat agtattcgat atccatttca atgcccatga
     1081 atatgcggcg gacagtgcct cccccatctc atcaacatca tctatattcc cagctccggt
     1141 gtaaacagct gtttgtccac catcgaagac ctttatacct tgtactatga ggtcacatcc
     1201 accaacataa tccattgagt agccagtttg tttaatgata tcagacattt ctcctataca
     1261 taggagaggc gcccccttat caagtaatac cttcaatgca ttactgactt cggatgaatc
     1321 ttgcgttttt acccatgttg catccaccaa aatgaccgcc tcattactca agactttcat
     1381 tggtagatcg tcaagagagg ttaaagtgat atctgatgag acttctatca ttttattgat
     1441 tattatatca taataatttc cacttccaag aataatgagc ggaatatttg aaataatatt
     1501 tatttcatca tcattttctt cattttgaac attaggcgat actgataatg caattacatt
     1561 cgttgtaacc attaatgtca caatgagcag tgcaactatc agattacaca atttgttcat
     1621 tgatcaatca ctcccccact aactacccga tcattactga ccgagcgaat tatcatcttt
     1681 tttttaagta tttataagtt cgttaatttt ttctggcctt gttgtagaac aacaatgacc
     1741 gggttgcctg gttactgtga tattgtaaca aggtcgatgt tgaataaaaa aagggagaaa
     1801 tggtttgaag tcagaaaccc tttgattgaa taaaataatt gacatactgg ctatcttcag
     1861 agtcgaatcc cgacacctgc accatcgatc aaccctgatc ccgatggccc cataccgctc
     1921 cgggccctcc ttcccttcgt atcggccctt ccccatgctt tcgggacggg gatcgggcgt
     1981 ccgttcaggc ggtgggcctc atgttcctga acgccttcac cgtcagcggg aacatcctta
     2041 tctttccgtc cctcccatcg gccatcatcc tctggatgtc gtaatcgtac tccttccggc
     2101 acatcctgat gacctcgctc gcctccacta tcccgaaccg caggatcacg ttgatcgcga
     2161 ccctggtcaa ggggttccta aggcttcggc cgccgtactg gccgaggatc ctcctcgccc
     2221 aatccaggtt atgctcccag caatcaagga aaagccgggt gtgcgccggg gtcatgtcct
     2281 ttgcctcgaa ggacctctct ccgttgacga actccgccgt cgccacgaag aacaggggca
     2341 cgatgagcga gcggtactcc cggaggcggt gcaccaggtc gatcgtcgcc agcacgtcct
     2401 cctccgtttc gcctggcaga cccatgatga tcgtcccgca cggaacccag ttgttctcct
     2461 gacagaccct gaacccggaa acgaccacgt ccggccattc ctccggcgag aagggcttgc
     2521 actttccctt catgtagtgc tgcatgaggg agggggaccc ggtctccacc cctgtctggg
     2581 cgcccaacca gttctcctcc gacagcccca tggtcccgga gatctccctg atcgcgtccg
     2641 gacaggaggc gacggaggcc agggcgaagt ggctgatgga gatcctctcg acgccctcca
     2701 ccgccttgac cgcccggaac agcgaggtca ggcggtcctc attgacagcc catccgttcg
     2761 ccccgtagcg gacgacgtcc tccgcgtgca gcaagggctg cctgccggcc ctg
//
LOCUS       DAOSYN010000175         2807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_27950_length_2807_cov_5.719113, whole genome shotgun sequence.
ACCESSION   DAOSYN010000175 DAOSYN010000000
VERSION     DAOSYN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27950_length_2807_cov_5.719113"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1328)
                     /locus_tag="PLJ11_06580"
     CDS             complement(240..1328)
                     /locus_tag="PLJ11_06580"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HOO04366.1"
                     /translation="MLIGILLVSVSALSMPALAADSDGMDVRNAGGSVAYANGHGQGN
                     LIFFDLNETSGELSDFGLNTSEGGRVLLHSLSIEGFVPEDITTMGSLARMTGENGTVV
                     LHDNPTGMVHVFMTDACEIAIVLAGNFTVTDRKEVGNATNVSYRLVLSDGRFEGTISS
                     DAPFEVSEDGMAVTCSARDLMVKFMPQLTLHPDWREALVMDAIQDGRVAMELALFSEG
                     AEGSYDVISYNKGLNAQVQKVERGRLQFVIGGENHQGAVLLVHTDKATLDNGNGLRVT
                     MNGDGMALVEEPVELLFEQKQQASYGVIDEGENQLMVVYLPVNSVGMITVESVDPLSD
                     LLSPLGLAMVIGAIGIVVLAAVAVFRRF"
     assembly_gap    1390..1489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1709..1918
                     /locus_tag="PLJ11_06585"
     CDS             1709..1918
                     /locus_tag="PLJ11_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04367.1"
                     /translation="MLVVSVIALRRPEGKGMLWVFVSFLGFTVLSTLALLGELFDDPT
                     WDLSNGVVLILLLVIGANYLLVLKG"
     gene            1923..2459
                     /locus_tag="PLJ11_06590"
     CDS             1923..2459
                     /locus_tag="PLJ11_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO04368.1"
                     /translation="MGNALELESRKMIYDFIHSRPGTYMREMERELGMGVGMLTYHLR
                     VLTEAGIIRVEKEGNHLRYFRSDDLHLEDRRTLSYLRDGPSRVILVYILDNGSVSVSQ
                     LKALTGMSSSTIYYHLKKLSSVGLVAISKGESITASVPDPDRMINMLLLVKEDIEKDP
                     AEALTSAWDRLKRLRPLT"
BASE COUNT          564 a          778 c          759 g          606 t
ORIGIN      
        1 gaggacgtgc cagccccaag cctcccttgg tcctaccaca cctcgcacat gccccttggg
       61 ctctccgctg gtgttaccga ttggggggcg gcattggaaa tgcccttgtt gccatgcacg
      121 ccgcggaaaa ggtccctaga acgatggggg agacgcgccc tgcctggcgg gtaccggccc
      181 ccggaggcga tgatcccctt ggggagggcc tatgctcagg aaagggattg agagcggggt
      241 tagaacctcc taaagaccgc cactgcggcg aggaccacga tcccgatggc cccgatgacc
      301 atggcaaggc ccaaggggga caacaggtcc gataacgggt ccacgctttc cacggttatc
      361 atgcccactg agttgaccgg caggtacact accatcagtt ggttttcacc ctcgtcgatc
      421 actccgtagg aggcctgttg cttctgttcg aacagcagct ccaccggttc ctccaccaag
      481 gccatcccat cgccgttcat cgtgacgcgc agcccattgc cattgtcgag ggttgcctta
      541 tcagtatgga ccagaaggac cgccccctga tggttctccc ctcctattac gaactggagc
      601 ctgcccctct ccaccttctg gacctgcgca ttgagcccct tgttgtacga tattacgtcg
      661 tacgaaccct ccgcgccctc ggagaacagc gccagttcca tggccactcg gccatcttgg
      721 atggcatcca tgaccagtgc ttccctccag tcgggatgga gggtgagctg gggcatgaac
      781 ttgaccatca gatcgcgggc ggagcatgtc accgccatgc cgtcctcgct cacctcgaag
      841 ggcgcgtcgg aggagatcgt gccctcgaag cgcccgtccg ataggaccaa ccgatacgag
      901 acgttcgtgg cattgcccac ctctttcctg tcggtgaccg tgaagttccc cgcgaggacg
      961 atggcgatct cgcaggcatc cgtcatgaag acatggacca taccggtggg gttgtcatgg
     1021 agaacaacgg tgccgttctc acccgtcatc ctggccagcg agcccatcgt tgtgatgtcc
     1081 tcgggaacga acccctcgat cgaaaggctg tgcaggagca cccttccgcc ctcggacgtg
     1141 ttcaagccaa aatcggatag ctcgcccgat gtctcgttga gatcgaagaa gatgaggttg
     1201 ccctggccat gcccattggc atatgccacg ctgcctccgg cattccttac gtccatccca
     1261 tcggagtcgg cggccagtgc tggcatggag agcgcactga ccgatactaa caggatgcct
     1321 atcagcatgt agctaaataa acgagccctt gtgatcaccc acacctaaga aaaacgatgg
     1381 tgtggagatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aataaacgag
     1501 cccttgtgat cacccacacc taagaaaaac gatggtgtgg agatgccatt atgattttgg
     1561 tacgcgggtt gaaagatcca ttgagttccg ttccgatggc cgtgaccaaa tgcattatga
     1621 ttaggagcat tccatattca tcaggggaag aggtatggaa ccgcttgctc tagctttgaa
     1681 tataatcctc cttggcctga gcgtcatcat gctggtggtc agcgtgatcg cccttcgccg
     1741 gcctgaagga aaagggatgc tctgggtctt cgtctcgttc ctgggtttta cagtcctttc
     1801 aacgttggca ttgctgggag agctgttcga tgacccgacc tgggatcttt cgaacggtgt
     1861 cgtattgata ttattgcttg taataggcgc caattacctt cttgttctga agggttgata
     1921 ggatgggcaa tgccttggag cttgaaagtc gaaagatgat atacgacttc atccactcca
     1981 ggccaggcac atacatgagg gagatggaga gggagctggg gatgggggtg gggatgctca
     2041 cctatcatct cagagtgctg acggaagcgg ggatcataag ggtggagaag gaggggaacc
     2101 atctccgtta tttccggtcc gatgacctcc atctcgagga ccgtcgaacg ctttcctatc
     2161 tgagggatgg gccgtcacgg gtcattctgg tgtacatcct ggataatggt tcggtgagcg
     2221 tttctcagtt gaaggccctg acggggatgt cgtcctccac gatatattac catctgaaga
     2281 agctctcatc cgtcgggctc gtcgcgatca gcaagggcga gagcataacc gcgagcgtcc
     2341 ccgacccgga ccgcatgatc aacatgctgc tattggtgaa ggaggatatt gagaaagacc
     2401 ccgccgaggc cctgacctcg gcctgggaca gactgaagcg cctcaggcct ttgacctgag
     2461 gagagggacc cgcctttaac gttcgatcaa cgctccctat ccggtcggac cggggtcgtt
     2521 ctttaataca tgggctggtc ttggagtttt agagttccca tagtgctttt tccatctact
     2581 taccctgaca gtcagggttc cgatgttcta cgaggggaga cgacgttccc ctcacgccct
     2641 catctccgat ccgtcgcgcc aagcccctta cggccatgcg aggcgctcgg catccatctc
     2701 catgggtcct tgatggggga tgccgcaacc ccaccgaatg aaggctgtta tcgaatttta
     2761 gcacctatgc taaaagtctc cccgttatcg gccttatcat catcctc
//
LOCUS       DAOSYN010000176         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_28246_length_2782_cov_4.331500, whole genome shotgun sequence.
ACCESSION   DAOSYN010000176 DAOSYN010000000
VERSION     DAOSYN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28246_length_2782_cov_4.331500"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2314
                     /gene="alaS"
                     /locus_tag="PLJ11_06595"
     CDS             <1..2314
                     /gene="alaS"
                     /locus_tag="PLJ11_06595"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOO04369.1"
                     /translation="CVRFNDIDNVGKTGRHFTFFEMCAHHAFNKEGRFIYFKDRTVEL
                     CHRFFTERLGTDPGKMRYVEEWWEGGGNAGPCVEVILDGVEVATLVFMMYRDTPEGRK
                     PMEMTVVDTGYGLERMTWVSQGVSSAYEAVFGPVVEWLKGLACVRPDPLVLTEYSKVA
                     GATNMRTAADVRRIRETTAERIGIGYEELMRTVAPLEDIYVICDHSRALMLMLNDGVV
                     PSNVREGYFARMLVRRALRSIRSLDLDIRLAEVVSRQIDHFQDIFPELRENREDILGL
                     VQVEEDRYFETLGRGKQLVDRLVKDLREGEKLPVERLIELYDSHGLNPEIAKEFAPDR
                     IDVPDNFYIQVAARHERPEAATEASGDRPERMPETRMLYYEDPELVEFEGTVIAEVDG
                     GVVLDQTAFYPEGGGQEWDLGTLDGKKVCRVIKIKTAIVHFVEGENPKIGAKVRGTID
                     LERRQQLMRHHTAAHLINGVARTLFGNHVWQAGAHKAVDQARLDITHFENLSTEQRDL
                     LEREVNRVVLQDRPVHIQFMQRDQAERLHGYRLYQGGAVPGKVIRVVEVEGLDAEACG
                     GLHCSHTGMVGPVRIARTKRIQDGVVRIEYTAGMAAVAGMQADKRLVEGLGDMLNVPA
                     EGLPEAVGRLQEELREQRKGIERLSAAFNELSVKALVGSAPKVGSVRVVVHEALPGED
                     PEEMSKRLASVPGTVAVIGVPGERARLLVSRSSDVKLDCRKALKRMMAVMGGGGGGKP
                     DFAQGGGGDASRLRDALAGAFEIISKEIEG"
     gene            2532..>2782
                     /gene="rpsM"
                     /locus_tag="PLJ11_06600"
     CDS             2532..>2782
                     /gene="rpsM"
                     /locus_tag="PLJ11_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOO04370.1"
                     /translation="MAEAKKSGNEEKEDFRYIVRIVNTDIDGNKPTVVGLQSVKGVGK
                     RVAEIVVQKAGVDRLEKIGALDEATTDELARLITTYSEYA"
BASE COUNT          556 a          759 c         1013 g          454 t
ORIGIN      
        1 atgcgtacgg ttcaatgaca tcgacaacgt tggaaagacc ggaaggcatt tcacgttctt
       61 cgagatgtgc gcccaccacg cctttaacaa agaaggtagg ttcatctact tcaaggaccg
      121 cacggtggag ctgtgccatc gtttcttcac cgagaggctg ggcaccgatc ccgggaagat
      181 gcgctatgtt gaggaatggt gggagggcgg cggcaacgcg gggccttgcg tcgaggtgat
      241 cctggacggg gtggaggtgg ccaccctggt attcatgatg taccgggaca cgcccgaggg
      301 gcgcaagccg atggagatga ccgttgtgga cactgggtat gggctggagc gcatgacctg
      361 ggtctcccaa ggggtgtcct cagcctatga ggcggtgttc gggccggtcg tcgaatggct
      421 caagggcctg gcctgcgtga ggcccgaccc gctcgtactg accgaataca gcaaggtggc
      481 cggggccacc aacatgagga ccgcggcgga tgtgcgccgg ataagggaga ccacggctga
      541 gcgcattgga atagggtatg aggagctgat gcggaccgtc gcccccctgg aggacatcta
      601 cgtgatctgc gatcattccc gagcgctcat gctcatgctc aacgacggcg tggtgccctc
      661 aaatgtccgt gagggttact tcgcccggat gctggtccgc cgcgcgctgc ggtccatccg
      721 ctccctggac ctcgacatca ggctggccga ggtcgtcagc aggcaaatag atcatttcca
      781 ggacatcttc ccggagctga gggagaaccg cgaggacatc cttggcctcg tgcaggtgga
      841 ggaggaccgc tactttgaga cgctgggaag gggcaagcaa ctggtagaca ggctggtcaa
      901 ggacctgagg gagggggaga agctgccggt ggagaggctc atcgaactct acgattccca
      961 tggtctgaac ccggagatcg ccaaggagtt cgcaccggac cggatcgacg tcccagataa
     1021 tttctacata caggtggccg cccgccatga gaggccggag gcagcgaccg aggcttccgg
     1081 ggaccggccg gagaggatgc cggagaccag gatgctgtac tacgaggacc ccgagctggt
     1141 ggagttcgag gggaccgtga tcgccgaggt ggacgggggg gtggtcctgg accagacagc
     1201 cttctatccg gagggcgggg ggcaggagtg ggaccttggg accttggacg ggaagaaggt
     1261 atgccgggtc atcaagatca agaccgcgat cgtgcatttc gtcgaggggg agaacccaaa
     1321 gatcggggcc aaggtgaggg gaaccatcga tctggagcgc cgccagcagc tgatgcgcca
     1381 ccataccgct gcgcatctga tcaacggtgt tgcgcggacc ctcttcggca accacgtctg
     1441 gcaggcgggg gcccacaagg cggtcgacca ggccaggctg gacatcacgc acttcgagaa
     1501 cctcagcacc gagcagcgcg acctgctgga aagggaggtc aaccgcgtcg tgctccagga
     1561 ccgccctgtc cacatccagt tcatgcagag ggaccaggcg gagaggctgc atgggtacag
     1621 gctgtaccag ggcggggccg ttcccggaaa ggtcatccgg gtggtggagg tggaggggct
     1681 ggacgccgag gcctgcgggg ggctgcactg ctcccacacc ggcatggtcg ggccggtcag
     1741 gatcgcccgc accaaacgca tccaggacgg ggtggtccgc atcgagtaca ccgcaggcat
     1801 ggcagcggtg gccgggatgc aggccgacaa gcgcctggtg gaggggctag gggacatgct
     1861 caacgtgcct gcggaagggc tgccggaagc ggtgggcagg ctccaggaag agctgcggga
     1921 gcagcgcaag ggcattgagc gcctttccgc ggccttcaac gagctgagcg tcaaggccct
     1981 ggtcgggtcc gcgcctaagg tcggctcggt ccgcgtggtg gtgcacgagg cgctcccagg
     2041 cgaggacccg gaggagatgt ccaagaggct ggcctcggtg ccgggcacgg tggcggtgat
     2101 aggcgtgccc ggggaaaggg cccgactgct cgtctcccgt tcctccgatg tcaagctgga
     2161 ttgccgtaag gccctgaaac ggatgatggc ggtcatgggc ggcgggggag gcggaaagcc
     2221 tgactttgcg cagggagggg gcggggacgc ctcccggctg agggacgccc tggcgggggc
     2281 cttcgagatc atctccaagg agatcgaggg ttgagccttc caaacatccc cgccatcacg
     2341 caatccttaa atatggattg agggttggcc ttccacacag aatcgattct gttggaggag
     2401 ccccttctga aaggggcttg ttcccggacc ggtagcccta tccggagatg ggaactgcaa
     2461 accgtgcgct ttggcacggg gcgtacttcg cgagggcgag gctcgattcg cgatcatcag
     2521 cgagggatac attggctgag gcaaagaaga gcggcaatga ggagaaggaa gactttcggt
     2581 acatagtccg catcgtgaac acggacatcg acggcaacaa gcccaccgtg gtcggccttc
     2641 agagcgtcaa gggcgtggga aagcgggtgg ccgagatcgt cgtgcagaag gccggggtgg
     2701 accggctgga gaagatcggg gccttggatg aggccacgac cgatgaattg gcgaggctca
     2761 tcacgaccta ttcggagtat gc
//
LOCUS       DAOSYN010000177         2762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_28479_length_2762_cov_3.461027, whole genome shotgun sequence.
ACCESSION   DAOSYN010000177 DAOSYN010000000
VERSION     DAOSYN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28479_length_2762_cov_3.461027"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..749
                     /gene="msrB"
                     /locus_tag="PLJ11_06605"
     CDS             357..749
                     /gene="msrB"
                     /locus_tag="PLJ11_06605"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOO04371.1"
                     /translation="MGRKVERTEEEWRRELDPEQYRVLRRKGTEAPFTGRYYRTKDKG
                     TYLCAGCGRELFASEDKFDHGCGWPSFSRPMGDLDERRDLSHGRARTEVLCPDCGGHL
                     GHVFDDGPAPTGQRYCINSASLRFRPRE"
     gene            755..1846
                     /gene="corA"
                     /locus_tag="PLJ11_06610"
     CDS             755..1846
                     /gene="corA"
                     /locus_tag="PLJ11_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOO04372.1"
                     /translation="MEIAGSRSMRRHANKKVGMPPGSMIYVGDGSPSPTTVSLMDYTE
                     TEVMEKTGVTLEECMPLRDSPSVTWLNFSGLADVETIKRLGEVFGLHPLVLEDILHIG
                     QRPKLEQYDRYVYLVVKMLYRGDNGEDNGKEVTYEQLSIVLGKNYVLTFQEREGDVFT
                     NIRERIRNNKGRLRRSGPDYLVYSILDMVVDNYFPALERVGDRVEDIEERMSDDPEPE
                     VLSDIYRLKREVLFLRKSIWPAREVVGAMMRQDIELVAPSTTTYLRDVYDHSIQVMDV
                     VETYTDMLSELTDVYMSMVSNRMNEIMKTLTIIATIFIPLTFIVGLYGMNFTYMPELT
                     SRYAYPAVLLGMATIAMVMLLYFRRKGWL"
     gene            1854..>2762
                     /locus_tag="PLJ11_06615"
     CDS             1854..>2762
                     /locus_tag="PLJ11_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOO04373.1"
                     /translation="MDASSVLAVGGIVLSLTFAFTNGYNDVGADVATMVASGAASVKG
                     ALLTASLANFIGAMVGGSAVVLTVQGILTADLAGSLGLGMFCAVLAATAWNAISWYYG
                     IPSSSTHALIGGLVGVGVALQGMAGVNWGTEELLAGQLVGVVKVIVFLLVSVLVGFGG
                     SFLLMKLARLALRRAKVGLNAHLRRLQWLTTGFQTLAHGANDAQKQMALIALALMTFG
                     NAPATVPLWARVACATAIGLGSLGGGYRIMRTVGRRIFKLRPVHALVVQANTSLTVAL
                     STAMGAPVSSTQVVTAGVMGVGSAENR"
BASE COUNT          512 a          817 c          956 g          477 t
ORIGIN      
        1 cccctgaggc ccactttttg ccctgtcaag gaggcccgcg cccatatggg ccaccctggc
       61 tggaaccgag gtctcattga aagggggatg ggcgtgcgac agtggcgatg gtgctgtccc
      121 cctgaaatgg agcgaacggc gaaaggatat cgcagatggt tgaaagctaa accccgggac
      181 cgtcagctcc ttccatggcg gggccgcggg ttggccctaa tggaaagggg cgggagcgga
      241 ggccgcagcc ggtatgggag cgatgccggg ggtacggcaa caggcggccg aagcatccgc
      301 acgggtctgg gagcaatgga gataaacatg gccgaccata tagccgcggg gtggcgatgg
      361 ggcgcaaggt cgagaggacc gaggaggagt ggaggcgaga gctggacccc gagcagtatc
      421 gagtcctcag gaggaagggc accgaggctc ccttcaccgg gaggtactac cgtacgaagg
      481 ataaagggac atacctgtgc gctgggtgcg gcagggagct gttcgcctcc gaggacaagt
      541 ttgaccatgg atgcggctgg ccaagcttca gcaggccgat gggggaccta gatgagcgga
      601 gggacctaag ccatggaagg gcgcggacgg aggtgctctg ccccgattgc ggcggtcatc
      661 tcgggcacgt cttcgatgac ggcccagcgc ccacgggcca gcgctactgc atcaactccg
      721 cctcgttgcg gttcaggccg agggagtgag gaccatggag atcgccggca gccggtccat
      781 gcgcaggcat gcgaacaaga aggtggggat gccccccggc tcgatgatct atgtggggga
      841 tggcagtccc tcccccacca ccgtgtccct catggactac acggagacgg aggtcatgga
      901 gaagacgggg gtgaccctgg aggagtgcat gcccctgcgg gacagtccct ccgtcacctg
      961 gctgaacttc tccgggctgg cggacgtgga gacgatcaag cggctggggg aggtcttcgg
     1021 gctgcacccc ctcgtcctgg aggacatcct gcacatcggg caaaggccca agcttgagca
     1081 gtatgatagg tacgtgtacc ttgtcgtcaa gatgctctac aggggggaca acggggagga
     1141 caacggcaag gaggtcacgt acgagcagct ctccatcgtc ctgggcaaga actatgtgct
     1201 gaccttccag gagagggagg gtgacgtgtt caccaacatc cgggagcgaa tacggaacaa
     1261 caagggccgc ctccggaggt cggggccgga ctatctcgtc tattccatcc tggacatggt
     1321 cgtggacaac tactttccag ccttggagcg ggtgggggat agggtcgagg acatcgagga
     1381 gcggatgtcc gacgacccgg aaccggaggt ccttagcgac atctaccgct tgaaacggga
     1441 ggtcctcttc ctgagaaagt ccatttggcc cgcgagggag gtggtggggg ccatgatgcg
     1501 ccaggacatc gagctggtcg caccgagcac gaccacctac ctccgtgacg tctatgatca
     1561 ctccatccag gtgatggatg tcgtggaaac gtacaccgac atgctctccg agctgacgga
     1621 tgtctacatg tcaatggtca gcaaccgaat gaacgagatc atgaagacgc tgaccataat
     1681 cgccaccatc ttcattccgc tgaccttcat cgtcggcctc tacgggatga acttcacgta
     1741 catgcccgaa ctcaccagca gatacgcgta ccctgcggtc ctcctgggca tggcgaccat
     1801 agcgatggtg atgctccttt acttcaggcg caagggctgg ttgtgaggtg ccgatggacg
     1861 cctcgtccgt tcttgccgtg gggggcatcg tcctttccct gaccttcgcc ttcaccaatg
     1921 gctacaacga cgtgggggcg gacgtggcca ccatggtggc ctccggcgcc gcctcggtga
     1981 agggggcgct gctcacggcc tccctcgcca acttcatcgg cgccatggtg ggagggagcg
     2041 cggtcgtcct gaccgttcag ggcatcctga cggccgacct cgccgggtcc ctgggcctgg
     2101 ggatgttctg cgccgtcctt gccgccaccg cctggaacgc catctcctgg tactacggga
     2161 tcccatcctc ctccacccac gccttgatag ggggattggt gggggtgggg gtggccctcc
     2221 agggcatggc gggcgtgaac tggggaacgg aagagctgct ggccggacag ctcgttgggg
     2281 tggtgaaggt catcgtattc ctactggtct ccgtcctggt aggcttcggg ggctccttcc
     2341 tcctgatgaa gctcgcccgg ctagccctca ggagggcgaa ggtgggcctg aacgcccacc
     2401 tgcggcgcct gcagtggctc accacggggt tccagaccct cgcccacggg gccaacgacg
     2461 cccagaagca gatggcgctg atcgccctgg cgctaatgac cttcgggaat gccccggcga
     2521 ccgtgcccct ctgggcccgg gtggcctgcg ccacggccat cggcctgggc agcctgggcg
     2581 gggggtaccg gatcatgcgc acggtgggaa ggaggatatt caagctccgg ccggtgcatg
     2641 ccctcgtggt ccaggcgaac acctcgctga cggtcgccct ctccacagcc atgggggccc
     2701 cggtctcctc cacacaggtg gtcacggccg gggtgatggg ggtggggtcg gcggagaaca
     2761 gg
//
LOCUS       DAOSYN010000178         2752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_28610_length_2752_cov_3.331479, whole genome shotgun sequence.
ACCESSION   DAOSYN010000178 DAOSYN010000000
VERSION     DAOSYN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28610_length_2752_cov_3.331479"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..573
                     /locus_tag="PLJ11_06620"
     CDS             97..573
                     /locus_tag="PLJ11_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04374.1"
                     /translation="MKVELYHASKYGNGEKVVAYLQGLLVAGGHQAEYRHIRDAKPKE
                     LPSADLYVFCAPTRVGKPIGKMRRFLKKARLPEGARYALIATHGEPAPDKKTGKMPTP
                     EEIAKYQQNLPAMEAILKEKGAVKVAELKVYVKNTIKGPLADGWEKVVEEFAAKLM"
     gene            complement(607..>2752)
                     /locus_tag="PLJ11_06625"
     CDS             complement(607..>2752)
                     /locus_tag="PLJ11_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOO04375.1"
                     /translation="NGQDLWYRTNAGGTWADPVILAGTGNTGLYPSLTLDDEGDPHIS
                     FYDASQKTLRYCRNTEGTFEHSVFASSHIIGMDSSIAVDHLGGVHISYLDSTSLDLKY
                     MYRDPGSSSFFSSTVDGDGDVGFATSLALDSNGDPHIMYIDETNPEHLRVKCATCSPW
                     NRENVECQGTVGIGNSLALEADGTAHVSYFDSTNEDIVYATNAGGEWNREVVSSGGSV
                     ISASSLVVDNAGNVHMVYVEDDNLVYANNVGGSWTRLVVDDNELTGAAIHIALDSLGK
                     VHICYNNFVDHNLKYASNADGQWKRTTIDDSSNSVGNPSGMALDQNDKVHICYYDSTA
                     KDLRYINNTGGTWARANLASGNDMGSYCDIAVDSQGKVHIVFYGATNQELYHITDASG
                     AWTNEILDSMGDVGRFCSVAMDEHDRLHAVYLQWGTFDLMYGSQTGNLWTSVRLDHLR
                     ETMYLDNSLALDKEGRPHIAYYDPTHSDLKYAEPFSEPSAPLNLEAETNESGVALSWD
                     DALWDHGYPIHEYRVYRGLSPGSMVLIDHLGATANEYLDQTAYGGTYYYRVAAVNDIG
                     VGQRSGEAEIAVEATMFAPGAPENLDADAGNGTVTLTWDRPDDDGGSPITGYNIYRGT
                     AEGYLVLLTTIGNVTTYTDDEVVNGQTYWYKVSAVNAVGEGAMTGMESATPTAEDKDD
                     DGGSNTMLYIIIAIMAIAAVAVAAVFFLRKRK"
BASE COUNT          502 a          819 c          853 g          578 t
ORIGIN      
        1 ttaaggctga ggtcttgggg gcgcccccgt ccatcgcgcc gccctcattg agatgcgttg
       61 aaatactccc gtgggcatcc aatcggtggg aaggccatga aggtcgaact ctatcacgct
      121 tcgaaatatg gaaacgggga gaaggtcgtt gcctatctgc aggggctgct ggtggccggg
      181 gggcatcagg cggagtaccg tcacatacgt gacgccaagc cgaaggagct gccttctgcc
      241 gacctctacg tgttctgcgc gcccacgcga gtgggaaagc cgatcggcaa gatgaggagg
      301 ttcctgaaga aggccaggct gcccgagggc gcgagatacg ccctcatagc cacgcatggc
      361 gagcctgctc ccgataaaaa gaccgggaag atgcccaccc cggaggagat cgccaagtac
      421 cagcagaacc tgccggccat ggaggcgatc ctcaaggaga agggggcggt caaggtggcc
      481 gagctgaagg tgtatgtgaa gaacaccata aagggccctc tggcggacgg ctgggagaag
      541 gtcgtcgagg aattcgccgc gaaactgatg taataaaggt aagatgggaa agggttttga
      601 gaggacctac ttcctcttcc tcaggaagaa cacggccgct acggcgacgg ccgcaatggc
      661 cattatggcg atgatgatgt acagcatggt gttgctgcca ccgtcgtcat ccttgtcctc
      721 ggcggtgggc gtggcgctct ccattccggt catggcacct tcgcccacgg cgttcaccgc
      781 gctgaccttg taccaatagg tctggccatt gaccacctcg tcgtcggtgt aggtggtcac
      841 attccctatg gtggtcagca gcaccaggta tccctcggcg gtcccacgat agatgttata
      901 cccggttatg ggcgagcccc cgtcgtcatc tggccggtcc caggttaagg tcaccgttcc
      961 gttcccggcg tccgcgtcca ggttctccgg ggcgcctggc gcgaacatcg tggcctccac
     1021 ggcgatctcc gcctcgccgc tcctctggcc tacgccgata tcgttgaccg ccgccacgcg
     1081 gtaatagtat gtgccgccat acgccgtctg gtccaggtac tcgttggcgg tcgcccccag
     1141 atggtcgatc aggaccatag accctgggga tagcccgcgg tataccctgt actcatgtat
     1201 aggatagcca tggtcccata gcgcgtcgtc ccagctcagc gctaccccgc tctcgttcgt
     1261 ctcagcttcc aggttcaggg gggccgaggg ctcggagaac ggttcggcgt acttcaggtc
     1321 gctgtgggtc ggatcgtagt aggcgatgtg cggccgcccc tctttgtcga gggccaggga
     1381 gttgtccagg tacatcgtct cccgtaaatg atccagcctc acggacgtcc agaggttgcc
     1441 ggtctggctg ccgtacatca ggtcgaaggt gccccattgg agatagacgg cgtgcagcct
     1501 gtcatgctcg tccatggcca cgctgcagaa cctgccgacg tcccccatgc tgtccaggat
     1561 ctcattggtc cacgctcctg aagcgtcggt gatgtggtac agctcctggt tggtggcgcc
     1621 atagaacact atgtgcactt tgccttggga atccacggct atgtcgcaat agctgcccat
     1681 gtcattcccc gaggcaaggt tcgccctggc ccaggtcccg ccggtgttgt tgatgtagcg
     1741 caggtccttg gccgtggagt catagtagca tatgtgcacc ttgtcgttct ggtccagcgc
     1801 cattcccgag gggtttccca cgctgttcga cgagtcgtcg atggtggtcc tcttccattg
     1861 cccgtcggcg ttggaggcgt atttgagatt gtggtctacg aagttgttgt aacagatgtg
     1921 aacctttccg agcgagtcca gggcgatatg gatggccgcg ccggtcagct cgttatcgtc
     1981 caccaccaat ctggtccagg aacccccgac gttgttggcg tataccaggt tgtcgtcctc
     2041 gacgtacacc atatgaacgt tccccgcgtt gtccacgacc agcgatgagg ccgatatgac
     2101 ggaaccgccg ctggaaacca cctcccggtt ccattcgccc ccggcgttgg tggcgtagac
     2161 gatatcctcg ttggtggagt cgaagtagct cacgtgcgcc gtcccgtccg cctcaagggc
     2221 cagggagttg cctataccca ccgttccctg gcattcgacg ttctccctgt tccagggcga
     2281 gcaggtggcg cacttgaccc ggaggtgctc cggattggtc tcgtctatgt acattatgtg
     2341 cgggtcgcca ttggagtcca gggccaggga ggtcgcgaaa cctacgtcgc catccccgtc
     2401 cacggtcgac gagaagaacg agctgctccc cgggtcccgg tacatgtact tgagatcgag
     2461 gctggtggag tccaggtagg agatatgcac tccgcccagg tgatccaccg ctatggagct
     2521 gtccataccg atgatatggg aagaggcgaa aacggagtgc tcgaaggtgc cctccgtgtt
     2581 ccggcaatag cgcagggtct tctggctggc atcatagaag gagatgtgcg gatccccctc
     2641 gtcatccaag gtcaaggagg ggtacagacc agtgttgccg gtcccggcca atatcaccgg
     2701 gtccgcccat gtcccgccgg cgttggtccg ataccagagg tcctgcccgt tg
//
LOCUS       DAOSYN010000179         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_28901_length_2730_cov_2.912897, whole genome shotgun sequence.
ACCESSION   DAOSYN010000179 DAOSYN010000000
VERSION     DAOSYN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28901_length_2730_cov_2.912897"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLJ11_06630"
     CDS             complement(<1..799)
                     /locus_tag="PLJ11_06630"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04376.1"
                     /translation="MIASLENVSKVFTNPRTGKGLVALNDVSMDVGEGDFLCLIGPSG
                     CGKSTTLNLLAGFELPTSGRVMFKGKEIAGPSPERGVVFQEFSLYPWMTVMQNVAFSV
                     SKGRTKEEREALAMKQLELVGLSDFAHARPNELSGGMKQGVAIARALAMDADLLLMDE
                     PFGSLDEQTRRKMDNEVLRLWEGQGKTVVFVTHSIEEALMLGTRILLMSASPGRINRE
                     WKVGAPWPRDPTSSGMVALKREISRGLQVCSCANGAPKAAFIEVQGTD"
     gene            complement(801..1667)
                     /locus_tag="PLJ11_06635"
     CDS             complement(801..1667)
                     /locus_tag="PLJ11_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO04377.1"
                     /translation="MDRKEAAEKALREGRSHFEFRPETRRFLLANAVNIAIGIALVII
                     AWWAVAEAMIAVRGAFFPTPPETFSALWEALGGAKINGATIYFHTRSSLVRWGLGYAL
                     ALVIGTIAGMAFGTMGRAYDIGMVPVYIVQMIPGMAWVPIAMLIFGLGESATVFIILM
                     TALPPIVINTAGGIRQAPPIYSRVALMSGKDHFSLFAKVLIPAAALPIIDGMRIGLAN
                     GWRVLIAAEMVLGTMVGLGATIEDSRYSLDFVSAFVCIIVICLIGLTIEKLLFSGLEG
                     VVRNRLGLEEEG"
     gene            1852..2163
                     /gene="ffs"
                     /locus_tag="PLJ11_06640"
     ncRNA           1852..2163
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLJ11_06640"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            complement(2167..>2730)
                     /locus_tag="PLJ11_06645"
     CDS             complement(2167..>2730)
                     /locus_tag="PLJ11_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOO04378.1"
                     /translation="EPGQAIFSHTAQVGDDVIIGMDLDGYYPDSLPFAWDTTSRKEPI
                     LLRRQMLIMNKIAKQGLVRSGKDISNPGTLGTLGMLLETSGKGAVVDVDLIPRPKDVE
                     LVQWLRSYQGFGYVVTCAPESSRQVIEMYASVGITAAVTGKVTSEPKLVVTQKDESAV
                     MFDFEREIITGCKPTSTVGWESVDLGK"
BASE COUNT          506 a          886 c          790 g          548 t
ORIGIN      
        1 catccgtccc ttggacctct ataaacgccg ccttgggcgc accgttggcg caggagcaca
       61 cctgcaggcc cctgctgatc tccctcttca atgccaccat tcccgaggag gtggggtcgc
      121 ggggccaggg cgcgcccacc ttccattccc tgttgatccg cccgggggag gcggacatga
      181 gcaggatgcg ggtgcccagc atcagcgcct cctctatgct gtgagtgacg aacaccaccg
      241 tcttcccctg cccctcccag agcctcagca cctcgttgtc catcttccgc ctggtctgct
      301 cgtccaggga accaaagggc tcgtccatca gcaggagatc ggcgtccatt gccaaggccc
      361 gggcgatggc caccccttgc ttcatccccc ccgagagttc gttcggccgg gcgtgggcga
      421 agtccgagag cccgaccagc tccagctgtt tcatggccaa tgcctcccgc tcctccttcg
      481 tcctgccctt gctcaccgag aaggccacgt tctgcatcac tgtcatccag gggtacaggc
      541 tgaactcctg gaagacgacc ccgcgctccg gagaggggcc ggcgatctcc tttcctttga
      601 acatcacccg ccccgatgtg ggcagctcga atcctgccag caggttcagc gtcgtgctct
      661 tgccgcagcc gctcggtccg atcaggcaga ggaagtcccc ttcgccgacg tccatgctga
      721 cgtcgttcag ggccaccagc cccttcccgg tgcgagggtt cgtgaagacc tttgacacgt
      781 tctcaaggga tgcgatcatg tcatccctcc tcctccaggc caaggcggtt gcgcaccact
      841 ccctcgaggc cggagaacag cagtttctct attgtcagtc ctatgaggca gatgacgatt
      901 atgcagacga aggccgagac gaagtcgagg ctgtacctgg aatcctcgat cgtggccccg
      961 aggccgacca tggtgcccag caccatctcc gcggctatga gcacgcgcca tccgttcgcc
     1021 agccctatgc gcatgccatc gatgatcgga agcgcggcgg cggggatgag caccttggcg
     1081 aaaagggaga agtggtcctt cccggacatc agcgccaccc gactgtagat cggcggggcc
     1141 tgccggatcc cccccgcggt gttgatcacg atcgggggca gcgctgtcat caggatgatg
     1201 aacaccgtgg ccgattcccc caggccgaag atcagcatcg ctattggcac ccaggccatc
     1261 cccgggatca tttggacgat gtagacgggc accatcccta tgtcatacgc cctgcccatg
     1321 gtgccgaagg ccatccctgc gatcgttccg atcaccaggg cgagggcgta gccgagcccc
     1381 catcggacca aggaggaccg ggtgtggaaa tagatggtgg cgccattgat cttggcgccg
     1441 cccaatgcct cccatagggc ggagaaggtc tccggaggcg tcggaaaaaa tgcgccccgg
     1501 acggcgatca tggcctcggc caccgcccac catgcgatga tcacgagcgc gatgccgatg
     1561 gcgatgttca cggcattggc gaggaggaac cgcctcgtct ccgggcggaa ctcaaagtgc
     1621 gacctgcctt cccgcaaggc cttttccgcg gcttcctttc tatccatctt tcctttatcg
     1681 tccactcgat ccgccttcca cattgagatt tcaaactatt tgattatgaa atcaatttat
     1741 taatgtaaaa ggtaaataaa ctattttata cttaacagga agcattggcc catgctcata
     1801 gccgcgcgcc cattccagaa tttataaata tggtcgaggg ataggtgaac ctgctaggct
     1861 tgaccgggac gttcggcgtg tccttgtaca ccgtaatcgt cgataggcgg gggtgacagc
     1921 gggggacgtc gtatccggag cgctagaggc ccaatcattg acgttgaggc cctgtcctac
     1981 ggggtcggtg gcagcatcgc ggtgcagctg gagggcggca ccccttgctc tagtcgtgga
     2041 gaacgggcta ggccctgacg ggagcagcct taccacggac tacctacgct cgaaggggag
     2101 cggggctgag ccagtggttg gacaacctct agcggtccga gcggcaaccc ccgtggccta
     2161 gcacctttat ttccccaggt ccacggactc ccatcccact gtggaggtgg gcttgcagcc
     2221 ggtgattatc tccctctcga agtcgaacat caccgcgctc tcatccttct gtgttacaac
     2281 taatttcggc tccgaggtca ctttgccggt gacggcagcg gtgatgccca ccgaggcgta
     2341 catctcgatg acctgccgcg aactctcggg ggcgcatgtc accacgtagc caaagccctg
     2401 ataggacctg agccattgca cgagctccac gtccttgggc ctggggatca ggtccacatc
     2461 gaccacggca cccttcccgc tcgtctccag gagcatgccg agggtcccca gggtcccggg
     2521 gttgctgatg tccttgccgg aacgcaccag gccctgcttt gcgatcttgt tcattatgag
     2581 catctgcctg cgcagaagga tgggttcctt cctggatgtc gtgtcccagg cgaatggcag
     2641 cgaatcggga tagtagccgt cgaggtccat cccgatgatg acatcgtccc ccacctgggc
     2701 tgtatggctg aatatggcct gccccggctc
//
LOCUS       DAOSYN010000180         2710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_29155_length_2710_cov_4.264407, whole genome shotgun sequence.
ACCESSION   DAOSYN010000180 DAOSYN010000000
VERSION     DAOSYN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29155_length_2710_cov_4.264407"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLJ11_06650"
     CDS             complement(<1..321)
                     /locus_tag="PLJ11_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO04379.1"
                     /translation="MKECMGCGKQLLDDMRFCPYCGRAMAEEDLERFEPARGEVVISV
                     ISPVRVTGREGTYALVLTKERLIFARIKDVAADKAKSELRQLGIFMPGSSGSDNVSRF
                     YEMAP"
     gene            505..864
                     /locus_tag="PLJ11_06655"
     CDS             505..864
                     /locus_tag="PLJ11_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOO04380.1"
                     /translation="MEGERISLRLEGEDLELIDEFIDGHPEISNRSQLARIALRAFIE
                     RDGGARAEGSGKGDLVVKVPPAVRSIMEGMVEDGYYNSVEDIVVECLRKEFVSKENLD
                     KVKDELFTKNRQIAARM"
     gene            988..1788
                     /locus_tag="PLJ11_06660"
     CDS             988..1788
                     /locus_tag="PLJ11_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04381.1"
                     /translation="MEQTVRTEQMVTMLSRGLTEPKISVVGCGGAGNNIVNNIFGSCR
                     KNVQTVAINTDERSLQKVNAHKKLLIGKDVTEGRGAEGFPEVGEYCAECAKDAIREAI
                     KDRDIVFVIAGMGGGTGTGTAPVVAQVAKDLNSITFVIAITPFSFETERANKAQMGIE
                     KLKRIAKTTVVVENDRLLSITDDLPLSKAFSVIDNSVVKIIDSFCSQVSMSFMTALTD
                     EVIQYMQESDRKAEERPEQYLVPEMVMVAKGPTENGLHPVGQMGPLVQ"
     gene            1906..>2710
                     /locus_tag="PLJ11_06665"
     CDS             1906..>2710
                     /locus_tag="PLJ11_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOO04382.1"
                     /translation="MLLGLVVNPIAGMGGSVALKGTDGDSYREAVTRGAVPVAQGKAT
                     RALAMIEGDVRFLTASGEMGASVLAALGQEHEVVFQVEGESGAEDTRRTCQAFLDRGV
                     DLIVFCGGDGTARDVLDVVGDKVPVIGIPAGVKMHSGVFANSPEEAGKIISAFLAERS
                     GTRMAEVMDVDEEAFRKGEVRARPYGYMKVPALEGIQPPKGAVFGTSDREQKEAIAEY
                     VVERMDPGTLYILGPGTTTKAVADRMGRPKTLLGVDAYLAGEIVLSDATE"
BASE COUNT          594 a          709 c          913 g          494 t
ORIGIN      
        1 gggggccatc tcatagaacc gtgatacgtt gtcggagccg ctggacccgg gcatgaaaat
       61 gccgagttgg cgaagctcgc tcttggcttt gtccgccgcg acgtccttga tgcgggcgaa
      121 gatgaggcgc tccttggtca ggaccagcgc ataggtgccc tcccttcccg ttacgcggac
      181 cggcgagatc acggagataa cgacctcccc cctggcaggc tcgaagcgtt ctaggtcctc
      241 ctcggccatc gctcggccgc aataggggca gaacctcata tcgtccagaa gctgcttccc
      301 gcagcccatg cattccttca tccatctcaa tggtatatgg ccagaggtat ttattgttcc
      361 atcgcctttc atatttttat gagaggatgt tcacggaagt ccctccccct taagtcagta
      421 tgacattgta tgacaaaagg cggacatttt aaatacttag tatgacaatc tgatttttag
      481 tctatgccaa gaggaccata cgctatggag ggagagagaa tatcgctgcg cctggaaggc
      541 gaggacctgg agctcatcga tgagttcatc gatgggcatc cagagatctc gaaccgttcg
      601 cagcttgcac ggatcgccct ccgcgcgttc atagaacgag atggaggagc gcgggccgag
      661 ggttctggca aaggcgattt ggtggtgaag gtcccaccgg cggtacgtag catcatggaa
      721 gggatggtgg aggacggcta ctacaattcg gttgaggaca tcgtggtcga atgcctgagg
      781 aaggagttcg tcagcaaaga gaaccttgac aaggtcaagg acgagctctt caccaaaaac
      841 aggcagatcg cggccaggat gtaggggcct tcggacctgc tctggacagg agcgcaaagg
      901 gatgggatgc actaattatc ttagcgtatg cgccgcaagg cgctgcgcgc aagaggaatg
      961 ggggcaaaag gaatggaagc cggaacaatg gaacaaacgg tcaggaccga gcagatggta
     1021 acgatgctca gccggggcct gacagaacct aagatatctg tggtggggtg tgggggtgct
     1081 gggaacaata tagtgaacaa catcttcggg agctgcagga agaacgtgca gacggtggcg
     1141 atcaacaccg atgagcgctc cctgcagaag gtcaatgcgc ataagaagct gctcatcggg
     1201 aaggatgtca ccgaagggcg cggggccgag ggattccctg aggtggggga atactgtgcg
     1261 gagtgcgcta aggatgccat aagggaggcc attaaggacc gggacatagt gttcgtcatc
     1321 gccgggatgg gcggaggcac cggaacggga acggccccag tggtggctca ggtggccaag
     1381 gacctgaact cgatcacgtt cgtcatcgcg atcaccccct tctcctttga gacggaaagg
     1441 gcgaacaagg ctcagatggg gatagagaag ctcaagagaa tagcaaagac cacggtggtg
     1501 gtggagaacg accgtctcct gagcatcacg gacgacctgc cgctgagcaa ggccttctcg
     1561 gtcatagaca acagcgtggt gaagatcatc gactcctttt gctcccaggt cagcatgtcc
     1621 ttcatgaccg ccctcaccga tgaggtcatt cagtacatgc aagagagcga ccggaaggct
     1681 gaggaacgcc ccgagcagta cctcgtgccc gagatggtca tggtcgccaa agggccgacc
     1741 gaaaacggac tgcacccggt cgggcagatg ggcccgttgg tgcagtgagc ggcccccggc
     1801 ttcctgaaac gttacaggta cggcgcgtgg caggcgccgt accatcccct aacctatcat
     1861 gccggttctc aaaggcctta tgtcgatggt gaacgtttcc gtggcgtgct ccttggtttg
     1921 gtcgtgaacc ccatcgccgg gatgggcgga agcgtggcgc tgaaaggcac cgacggggat
     1981 tcgtatcgag aggcggtgac caggggagcg gttccggtgg cgcagggaaa agccacccgg
     2041 gccctggcga tgatagaggg ggacgtacgc ttcctcacgg cctcggggga gatgggcgct
     2101 tccgtcctcg cggccctcgg gcaggagcat gaggtggtct ttcaggtcga gggggaaagc
     2161 ggggcggagg acacgcgccg gacatgtcag gccttcctcg acaggggggt cgacctgatc
     2221 gttttttgcg gcggggacgg cacggccagg gatgtgctgg acgtggttgg ggacaaggtc
     2281 cctgtcatcg gcatcccggc cggggtgaag atgcattcgg gcgtgttcgc caacagcccc
     2341 gaggaggctg ggaagatcat ctccgccttc ctggcggagc gttcgggcac gcgcatggcg
     2401 gaggtgatgg acgtggacga ggaggccttc cggaagggcg aggtgcgcgc ccggccatac
     2461 ggctacatga aggtccctgc cctggaggga atacagcccc ccaagggcgc ggtcttcgga
     2521 acatcggaca gggagcagaa ggaggccatc gccgagtacg tggtcgagag gatggaccct
     2581 ggaacgctat atatcttagg cccgggcacc accacaaagg ccgtggccga ccggatgggg
     2641 cggccgaaga cgctgctggg ggtggacgcc tatcttgccg gcgaaatcgt cctttccgat
     2701 gcgaccgaat
//
LOCUS       DAOSYN010000181         2684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_29484_length_2684_cov_4.990871, whole genome shotgun sequence.
ACCESSION   DAOSYN010000181 DAOSYN010000000
VERSION     DAOSYN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29484_length_2684_cov_4.990871"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLJ11_06670"
     CDS             <1..445
                     /locus_tag="PLJ11_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01210 family radical SAM protein"
                     /protein_id="HOO04383.1"
                     /translation="PFLTESAAIEDAKATVRFAAPFSESVSVNPLNVQKGTVVEGLWR
                     RGDYRPPWTWSLFEVLPLKRELPTVRLFSSPSGAGAQRGMHNCPSCDKGLLSALETFN
                     FEQDVGLLEGHDCACRREWASLLTAERAMRTSVDVRRYLEDELAL"
     gene            455..775
                     /locus_tag="PLJ11_06675"
     CDS             455..775
                     /locus_tag="PLJ11_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HOO04384.1"
                     /translation="MEYDIKKGNCGNLEGDGLRRLMEAAFGSVTEEGDAYVSSYGAMG
                     RIEVRMKGRTKLDISTVTDKNASPEVAADTIKRWNDFLERATGFTSKERRNRLQKKAK
                     DGKL"
     gene            1047..2048
                     /locus_tag="PLJ11_06680"
     CDS             1047..2048
                     /locus_tag="PLJ11_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOO04385.1"
                     /translation="MPNIRRPRRGSKGYGPRKRAKKQTPKLDSWPEISEGPKVQGFAG
                     YKAGMTHAFIVDRRKSSSTAGQEIRVPVTVLEVPPMKVAAVRIYESTRYGLRTAGEVW
                     APQLDEELSRLLPIPKKVEAEAAWERMKGVDVEDVRVLAFTQPKLVTGVPKKRPELME
                     LRIGGGPIEARIEFAKGLLGKDIAITDFAKSGAMIDVIAITKGKGFQGATKRWGLKLM
                     SHKNSKHRRAIGNLGPKRPGYVRTTVPQGGQMGYHQRTEVNKLILKVGESGQEVSPKG
                     GFLHYGEVRNPFVLVHGSVPGPTKRLVRMRDPVRRQQPDMKEAPEMVYISTESKQGA"
     gene            2052..>2684
                     /gene="rplD"
                     /locus_tag="PLJ11_06685"
     CDS             2052..>2684
                     /gene="rplD"
                     /locus_tag="PLJ11_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOO04386.1"
                     /translation="MSKVNVYSLSGEVLRTEALPKAFSTQFRPDVIHRAVVAEEANKR
                     QPYGPKPGAGARHSVSTWGKGRGVARVQRLAQGAKAAESPNNVGGRRAHPPRVERDWS
                     KKVNRKEKALAKMSALAATADAEKVRARGHRFKEGLTLPVVIEDRLEGIESTTDVLAA
                     LRNVGVSDDIARAKDGIRIRAGRGKMRGRPYRMPRSLLLVVSVHDAAVVKG"
BASE COUNT          567 a          764 c          937 g          416 t
ORIGIN      
        1 ccccttcctc accgagagcg cggccataga ggacgccaag gccaccgtgc gcttcgccgc
       61 ccccttctcg gagagcgtgt ccgtcaaccc actcaacgtg caaaagggca ccgtggtgga
      121 ggggctatgg aggcgaggcg actaccgtcc gccttggacc tggtccttgt tcgaggtgct
      181 tccgctcaag cgggagctgc cgaccgtccg gttgttctcc tcgcccagtg gggccggggc
      241 ccagcggggg atgcacaact gcccttcctg tgacaagggg ctgctgagcg cccttgaaac
      301 attcaacttc gagcaggacg tgggactgct ggagggacat gactgcgctt gccggaggga
      361 gtgggcatcc ctgctgacgg cagaaagggc gatgcgcacc tcggtggatg tgcggcgcta
      421 tctcgaggat gaactggcac tttagaggga ttctatggag tacgatatca agaaagggaa
      481 ctgcgggaac cttgagggcg acgggctgcg taggctcatg gaagccgcgt tcggctcggt
      541 aacagaggag ggggacgcat acgtctcctc gtatggggcg atgggccgga tcgaggtcag
      601 aatgaagggc aggacaaaat tggatataag tacggtcacg gacaagaatg cctctccgga
      661 ggtggcggcg gacaccatca agaggtggaa cgacttcctc gagcgggcca caggcttcac
      721 ctccaaggag cgccgtaacc ggctgcagaa gaaggccaag gatgggaagc tctaagagcg
      781 gccagtgaac gcctttccct cggccctcta tatatacatg gaccgattgg tttaaatacg
      841 aacgatagta tgcgaccttc gctcaacgca ctgaggacgt tcgaccagac cccttgggca
      901 atgcgcaagc atccctgagc cgcctccatg cgcacattct ggaggaaggc gacggtgcca
      961 cggtcgaagg tgctgacctc aacactcttg agaacaggcg ttagcgaagg agagtgtgct
     1021 ctctcgcgag acagggaggg agatcgatgc cgaacattag acgtccgaga aggggctcca
     1081 agggctatgg tcccaggaag cgagccaaga agcagacacc aaagctggat tcgtggcctg
     1141 agatcagcga aggcccgaag gtccagggat ttgccggtta caaggcggga atgacccacg
     1201 cattcatcgt ggaccgccgc aagagcagct ccacagcagg ccaggagatt agggtgcccg
     1261 tcaccgtcct ggaggtgccg ccgatgaagg tggccgcggt gcgcatctat gagagcaccc
     1321 gctatggcct caggaccgcg ggggaggtct gggccccgca actggacgag gagctgtcca
     1381 ggttgctgcc catccccaag aaggtcgagg cggaggcggc ctgggagagg atgaaggggg
     1441 tggacgtcga ggacgtcagg gtccttgcct tcacccagcc gaagctggtc actggggtgc
     1501 ccaagaagcg cccggagctg atggagctgc gcatcggagg cggccccatc gaggcgagga
     1561 ttgagttcgc caagggattg ctgggcaagg acatcgccat caccgacttc gccaagagcg
     1621 gcgcgatgat cgacgtcatc gccatcacca aggggaaggg cttccagggg gccaccaagc
     1681 gctggggcct gaagctcatg tcccataaga actcgaagca ccgccgcgcg atcggtaacc
     1741 tcggccccaa gagaccgggc tatgtgcgca cgaccgtacc ccagggcggc cagatgggat
     1801 accatcagcg gaccgaggtc aacaagctca tcctcaaggt aggggagagc gggcaggagg
     1861 tcagccccaa gggcggcttc ctgcactatg gcgaggtgag gaaccccttc gtcctggtcc
     1921 atggatccgt cccaggcccg accaagaggc tggtgcggat gagggacccg gtcaggcggc
     1981 aacagccgga catgaaggag gctccagaga tggtgtacat atccaccgag tccaagcagg
     2041 gggcgtaagc aatgtccaaa gtcaacgtct attcattgag cggcgaggtc ctgaggaccg
     2101 aggcactgcc caaggcgttc tccacccagt tcaggcctga tgtcatccat cgggccgtgg
     2161 tcgctgagga ggccaacaag aggcagccct atgggccgaa gccgggcgcg ggggcacggc
     2221 actccgtctc cacctggggc aagggccgtg gggtggcgcg tgtgcagcgg ttggcccagg
     2281 gggccaaggc cgctgagtcc cctaacaacg tgggcggccg ccgggcgcac cctccccggg
     2341 tcgagaggga ctggagcaag aaggtgaacc ggaaggagaa ggccttggcg aagatgtcag
     2401 ccttggccgc gaccgcggat gcggagaagg tcagggcgcg ggggcatcgg ttcaaggagg
     2461 ggctcaccct accggtcgtc atcgaggacc gtctcgaggg gatcgaaagc accaccgatg
     2521 tcttggcggc gcttaggaac gtcggcgttt cggacgacat cgcccgggcc aaggacggga
     2581 tcaggataag ggccggccgc gggaagatgc gtggccgtcc atacaggatg ccccgcagcc
     2641 tgttgctcgt cgtctcggtg catgatgccg cggtggtcaa gggc
//
LOCUS       DAOSYN010000182         2681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_29528_length_2681_cov_3.862148, whole genome shotgun sequence.
ACCESSION   DAOSYN010000182 DAOSYN010000000
VERSION     DAOSYN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29528_length_2681_cov_3.862148"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1912
                     /locus_tag="PLJ11_06690"
     CDS             <1..1912
                     /locus_tag="PLJ11_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOO04387.1"
                     /translation="EGGTVLSGRFVRGRVRYVLREDAPMYVKAYRTMALSETDQRVLR
                     RIREHGGASLRELAKGLPDAKETIKDSLDRLDRNMYIVRRYEEGEDWSRENVYEAYDA
                     PEYEGDPAEELVRRFVRAYGPVPPMAVRMYTGLPPEAVEGALDRLGVERIVVGDAATE
                     MVLMADELDALRAFSPPSEEWSVVSPYDPAAQPHWADINARFGDSWVFPVLRDGTVVG
                     GVEKWDVGGCVELRAIDLDEPSLLPMALQALEPLLRFQAAQGLDMVRVKEVLGVAADE
                     VQGEAAQALEAAGFIHLEGMWARGGVPRQYEREELLRYAMRRQGLLPRPAYPNVIEGV
                     KRTGGFRGDPAAFARCRVKVPLKRMVEQGLLYVVTGLPEHMMYTSMQHASLYRDAKAR
                     EISEDAQAMVRMLERNLPMPRRALFERSVLGPGRTQDALRELVRATVVAYGRNNRITL
                     VPPSGLDAREARLELLRLLFRNFGTFTAENLSRYLRGEVPMRELRSLLAQLTEEGFLA
                     KGFLERGSDAVHWALKEDMASIGRKDADRELVLYQFDNMAHYLYDEIRERCGGMGSLV
                     MRGPHIIGCFRSKHSGRDLTIIDLQGGREAKEVVKDFVSELGWTVREKTSKEIPDWEI
                     QEFLGKVMGREG"
     gene            complement(1914..>2681)
                     /locus_tag="PLJ11_06695"
     CDS             complement(1914..>2681)
                     /locus_tag="PLJ11_06695"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR00292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOO04388.1"
                     /translation="EVLVTRKIVERYTEEFLENVDVDVVIAGAGPASLTAARYLAKAG
                     LKVVIFERKLTPGGGMWGGGMTFPVIVVQEGSKALLEEIGVRLRDAGDGYYTADSVEA
                     SAKLIAAAVTAGARLYNTVSVEDVMIRGDGICGVVINSSAVEVAGLHVDPLAIRSKYV
                     IDGTGHPAEVVHVVQRKVGRLSTPTGQIEGERSMWAEVGEQMTVENTREVYRNLYVTG
                     MASNAVMGAPRMGPIFGGMLLSGRKVAELIIARERER"
BASE COUNT          434 a          851 c          983 g          413 t
ORIGIN      
        1 ggagggaggg acggtactca gcgggcgctt cgtccggggc agggtgcgct acgtgctcag
       61 ggaggacgcc ccgatgtacg tgaaggccta tcgtacgatg gccttgagcg aaacggacca
      121 gcgggtgctg cggaggataa gggagcacgg gggggcaagc ttgcgtgagc ttgccaaggg
      181 gctgccggac gccaaggaga caatcaagga ctcgctggac cggctggacc gcaacatgta
      241 catcgttcgg aggtacgagg agggcgagga ttggtcccgg gagaacgtct atgaggcgta
      301 tgacgcgccc gagtatgagg gggacccggc ggaggagctc gtgcgccgct tcgtccgggc
      361 ctacgggccg gtcccgccca tggccgtccg catgtatacg gggctccctc cggaggcggt
      421 cgagggggcc ttggaccggc tgggggtgga aaggatcgtc gtgggggacg ccgccacgga
      481 gatggtgttg atggccgatg agctggacgc cctgcgggcc ttctcgcccc ctagcgagga
      541 atggagcgtg gtcagcccgt acgaccctgc ggcccagccg cactgggcgg acatcaatgc
      601 ccgcttcggg gactcctggg tgttcccggt gctgcgcgac gggacggtgg tcgggggcgt
      661 ggagaagtgg gacgtgggcg gctgcgtgga gctgcgggcg atcgacctgg acgagccctc
      721 cctcctgcca atggccctgc aggccctgga accgctgctc cggttccagg cggcccaggg
      781 gctcgacatg gtgagggtga aggaggtgct cggggtcgcg gcggacgagg tgcagggcga
      841 ggccgcccag gccttggagg ccgcaggctt catccatctg gaggggatgt gggcccgcgg
      901 aggcgtcccg cgccagtacg agcgggagga gctgctgcgc tacgccatgc gccggcaggg
      961 gctcctcccc aggccggcgt acccgaacgt catcgagggg gtgaagcgca ctggcggctt
     1021 ccgcggcgac cccgcggcct tcgcccgctg ccgggtcaag gtcccgctga agcgcatggt
     1081 ggagcagggg ctgctctacg tggtcaccgg gctgcccgag cacatgatgt acacgagcat
     1141 gcagcacgcc tcgctctaca gggatgccaa ggcccgagag atcagcgagg acgcgcaggc
     1201 catggtcagg atgctggaac ggaaccttcc catgccgagg agggccctct tcgagcgctc
     1261 ggtgctcggt cccggccgga cgcaggacgc gctccgggag ctcgtccggg cgaccgtcgt
     1321 ggcgtacggc cgaaacaacc ggatcacgct cgtgcccccc tccggcctcg atgccaggga
     1381 ggcccgcctg gagctgctcc ggctgctgtt ccgcaacttc gggaccttca ccgctgagaa
     1441 cctgtcccgc tacctgcgcg gggaggtccc aatgcgcgag ctgcgctcgc tcctggcgca
     1501 gctgaccgag gaggggttcc tggccaaggg cttcctggag cgggggagcg acgcggtgca
     1561 ctgggcgctc aaggaggaca tggcgagcat cggcaggaag gacgcggaca gggagctggt
     1621 gctctatcag ttcgacaaca tggcccatta cctctacgac gagatcaggg agaggtgcgg
     1681 ggggatgggc agcctggtca tgcgcggccc gcatatcata gggtgcttcc gctccaagca
     1741 cagcgggagg gacctcacga tcatcgacct gcaggggggc agggaggcga aggaggtggt
     1801 caaggacttc gtttcggagc tgggatggac ggtgagggaa aagacgtcga aggagatccc
     1861 ggactgggag atccaggagt tcctggggaa ggtgatgggc agggagggtt gaactacctc
     1921 tcccgttccc gggcgattat cagctccgcc accttcctgc ccgacagcag catgccgccg
     1981 aagatggggc ccatgcgcgg ggcccccatg accgcgttgg aggccatccc ggtcacgtag
     2041 aggttcctgt agacctcccg ggtgttctcc accgtcatct gctcccccac ctcggcccac
     2101 atggaccgct ccccctcgat ctgcccggtc ggcgtgctca ggcggccgac cttccgctgc
     2161 accacgtgca cgacctcggc gggatgcccc gtgccgtcga tgacgtactt ggaacgaatg
     2221 gccagcgggt cgacgtgcag gcctgcgacc tccacggcgc tggagttgat gaccaccccg
     2281 cagatgccgt ccccgcggat catgacgtcc tccacggaga cggtgttgta gaggcgggcg
     2341 ccggcggtca ccgccgcggc gatgagcttg gcggacgcct cgacggaatc ggcggtatag
     2401 taaccgtccc cggcgtccct caggcggact ccgatctcct ccaggagggc cttgctgccc
     2461 tcctgcacca cgatgaccgg gaaggtcatg cctccccccc acatcccccc gccgggggtg
     2521 agcttccgct cgaagatgac gaccttgagc ccggccttgg ccaggtagcg cgccgccgtc
     2581 aatgaggccg ggccggcgcc ggcgatgacg acgtcgacgt ccacgttctc caggaactcc
     2641 tcggtgtacc tctccactat cttgcgggtg accagaacct c
//
LOCUS       DAOSYN010000183         2674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_29640_length_2674_cov_2.573501, whole genome shotgun sequence.
ACCESSION   DAOSYN010000183 DAOSYN010000000
VERSION     DAOSYN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29640_length_2674_cov_2.573501"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLJ11_06700"
     CDS             complement(<1..735)
                     /locus_tag="PLJ11_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04389.1"
                     /translation="MAHEWLDGLRKYGIIISRPVMAEAFPDGPAALDPAGLEGLRASF
                     RQFEAGVLSIEDWLWAIAQGLMGVENRPLLAFGEEARKHLELLPGMRGLTPPDGLLLS
                     YCEGTFTPRLYIWTVGDGGKRAGRWGRTCAEAQRFIRENQRKGVRLGLVTDGLRLRLY
                     HVDLEGEAWMEWDASNWFEEAGRQEEMRGFALMLSKGMLGIGSKDEPPIEALARQSRR
                     KQGDISSQLGEWLRRAVEEMMNKVVNS"
     gene            complement(737..>2674)
                     /locus_tag="PLJ11_06705"
     CDS             complement(737..>2674)
                     /locus_tag="PLJ11_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO04390.1"
                     /translation="DPGQRDLFGSEKIVRVIRKLEVDSKADIGDDRVREDMERDASSL
                     SGELLSRHWATLGPIAERITQLLSTEMRIGHDHVVTEDDKRPLDVPDSKFQALMSWLR
                     EVLGFDGKGIATEERAIIFTEYRDTQDYLLKRMLDAGVPRGSILIVNGGSDPGQIEGV
                     KEAFNDPRHCSRILLATDAAREGLNLQQTCRYVLHHDLPWNPLRIDQRVGRVDRHGQT
                     RKVRTWHHVLDTIYEYEFLSHIVHKVEEARSDLGSLSPILEESIESIFISDAGQPNPA
                     ETLKDVDELVSHDISTMGGRRTDYTEMKLGLDRAMDILEYSEDELRQTVDSALRLDGG
                     ALNETSEKGIYSIRFPQTWAHVEGSLRQPVTTAMLKVTFQRSAFLRTENGMQKWDERK
                     DLVLLSLGHPVVRHAIERLRRHLWQSRGGSRGGITRWSIDRPPEECDRAVVTLLKMAR
                     NARGEVVKEEAEQHLYDSRTKSLSPVTMSARPSSTSQTPPPPWHSELLTVLKADSEER
                     KRTFAAQVKEELRDHLEKDIQDYNDWKMRSVRMLEEATRDAVQTNLDNLDKEIRRLEN
                     EKKQRRLDPIIDVKIRRDLEQKKLERKELEERLSAGKLAELKAHIEYEADRFINEIFP
                     SRYTLGHVDLIVAGILFTGGD"
BASE COUNT          445 a          899 c          725 g          605 t
ORIGIN      
        1 ggagttcacc accttgttca tcatctcctc gaccgcgcgg cgcagccatt ctccaagctg
       61 ggaggatatg tccccctgct tccttctgct ctggcgggcc agagcctcta tggggggctc
      121 gtccttggac ccgatgccga gcattccctt gctgagcatg agggcgaacc cccgcatctc
      181 ctcctgccgg cccgcctcct cgaaccagtt ggaggcgtcc cactccatcc aagcctcccc
      241 ctccaggtcc acatggtaca gcctgaggcg aagcccgtcc gtaacgagcc cgaggcgcac
      301 ccccttccgc tggttctccc tgatgaaccg ctgagcctcg gcacaggtcc ttccccacct
      361 ccccgccctc ttccccccgt ccccaacggt ccagatatag agcctcgggg tgaaggtgcc
      421 ttcgcaatag gaaaggagca gcccgtccgg cggggtcagg cccctcattc cagggagcag
      481 ttcgaggtgc ttcctggcct cttcaccgaa ggccaggagg gggcggttct cgacccccat
      541 gaggccctgt gcaatggccc agagccaatc ctcgatgctc aaaaccccgg cctcgaactg
      601 cctaaaggat gccctcagcc cctcgagccc tgcggggtct aaggcagcgg ggccgtcggg
      661 gaaggcctcg gccatcactg gcctggagat gatgatgccg tacttcctca ggccgtcgag
      721 ccactcgtgc gccatctcaa tcgccccctg tgaataggat cccggcgacg atcaggtcca
      781 catggcccaa tgtgtagcgg gaagggaaaa tctcgttgat gaagcggtcg gcctcgtatt
      841 cgatgtgcgc cttcagttcc gccagcttgc ccgcggagag gcgctcctcc agctccttcc
      901 gctccagctt cttctgctcc agatccctcc ttatcttcac atcgatgatg gggtccagcc
      961 tcctctgctt cttctcgttc tcgagcctcc ttatctcttt atcaaggttg tccaggttcg
     1021 tctggaccgc gtcccttgtg gcctcctcga gcatccgtac gctcctcatc ttccaatcat
     1081 tataatcctg gatgtccttc tcgagatgat cgcgcagttc ctctttgacc tgtgcagcga
     1141 aggtgcgctt cctttcctcg gagtcagctt tgaggacggt cagcagctcg ctgtgccaag
     1201 gcggcggagg ggtctgagag gtagaggagg gccgtgcgct catggtgact ggcgacaaag
     1261 acttggttcg ggaatcgtag agatgctgct cggcctcctc cttgacgacc tcgccccggg
     1321 cgttcctcgc cattttgagc agggtgacga cggcgcggtc gcattcctct gggggcctgt
     1381 cgatgctcca gcgggtgatg cctcccctgc tgccgcccct cgattgccac agatgcctgc
     1441 ggagcctctc gatcgcatgc cggaccacag gatgccccag gctgagcagc actaggtcct
     1501 tcctctcgtc ccacttctgc attccgttct cggtccggag gaaggccgat ctttggaagg
     1561 tcacctttag catcgctgtg gtgaccggct gcctgaggga tccctccacg tgcgcccatg
     1621 tctgaggaaa acgaatcgaa tagatcccct tctcagaggt ttcgttcaac gcccccccgt
     1681 ccaggcgcag ggcggagtcg accgtttgcc gcaactcgtc ctcggagtac tccaggatgt
     1741 ccatggcccg gtccagcccc aacttcattt cggtatagtc ggtccttctc ccccccatcg
     1801 tcgatatgtc atgcgagacc agctcatcca cgtccttcaa ggtctcggcc gggttcggct
     1861 ggcccgcatc ggagatgaag atggactcga tgctttcctc cagtattgga gataggctgc
     1921 cgaggtcgct ccgggcctcc tcgaccttgt ggacgatatg agagaggaat tcgtactcgt
     1981 agatcgtgtc caggacatgg tgccatgtcc tgaccttcct ggtctggccg tgcctgtcca
     2041 ccctgcctac cctctgatct atcctcaatg ggttccaagg caggtcgtga tggaggacgt
     2101 atctgcaggt ctgctgcagg ttcagtccct ccctggcagc atcagtggcc aggaggatgc
     2161 gggagcagtg ccttggatcg ttgaacgctt ccttcactcc ttcgatctgg ccgggatcgc
     2221 tgcctccgtt cactatcaag atgcttcccc tgggcacgcc cgcgtcgagc atcctcttga
     2281 gcagatagtc ctgcgtgtcc cgatattcgg tgaagataat cgccctctcc tccgtggcaa
     2341 tgccctttcc atcgaaaccg agcacctccc tcaaccaaga catcaatgct tggaacttgg
     2401 agtccggcac gtcgagagga cgcttatcat cctccgttac cacatggtca tgcccgatgc
     2461 gcatctccgt gctcaacagc tgtgttatcc tttctgcgat cggccccagc gtcgcccaat
     2521 ggcgggatag cagttcgcca gagaggcttg aggcgtcccg ttccatatcc tcacggacgc
     2581 ggtcatcgcc gatatccgcc ttggaatcca cctccaattt tcttatcacc cggactatct
     2641 tttctgaacc gaagaggtcg cgctgacctg ggtc
//
LOCUS       DAOSYN010000184         2650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_29976_length_2650_cov_3.310212, whole genome shotgun sequence.
ACCESSION   DAOSYN010000184 DAOSYN010000000
VERSION     DAOSYN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29976_length_2650_cov_3.310212"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..1663
                     /locus_tag="PLJ11_06710"
     CDS             341..1663
                     /locus_tag="PLJ11_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOO04391.1"
                     /translation="MKHGMGTKFALILALALVALPLFSSPVAAADGDVDSGDTAWLMI
                     STGLVFVMTPAVAFFYGGMLRKSNFLSMLGQSILIIGVVTIIWVVFGYSLTFGADNGG
                     LIGGAEYLMLNSVNLAPNLIAPTVPHILFMMYQGMFAIITVALIIGGVAERMKLSSLM
                     VFLSIWTVAVYIPVAHWVWGGGWIYDLGALDFAGGTVVHITAGVSVLAAAMVLGKRIS
                     HAGGGQEVPHNIPMVVLGGALLWIGWFGFNGGSALASNGLAANALVVTHISAAMAATV
                     WGLISWLDTGRVSVLGLISGGVAGLVAITPAAGFVNPTGALFIGLGAAVVCYGGILLR
                     KRLGFDDALDVWGVHGLGGTFGAIATGIFATTTVNAAAKDGLLYGGGVDLLVAQLIAV
                     AAVWAFAFGVTYVVLKAMSKVMTLRMTKDEERIGADIIQHGESAYYLR"
     gene            1660..2007
                     /locus_tag="PLJ11_06715"
     CDS             1660..2007
                     /locus_tag="PLJ11_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOO04392.1"
                     /translation="MRKTKKIEAIIRSETLDSVKCGLEKLGMNAMTITDVLGRGEQKG
                     LEFTHRAGKYRVDLLPKVKVEIVVRDEDVRKVIAVIIEKARTGEIGDGKIFVSPVEEV
                     IRIRTGESSNGKA"
     gene            2170..>2650
                     /locus_tag="PLJ11_06720"
     CDS             2170..>2650
                     /locus_tag="PLJ11_06720"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOO04393.1"
                     /translation="MRALAKDIGKGHQLALDLWRTGVHEALILSSLLAESEKLTEEQT
                     KEMVRGFDSRDLCDQCCSNLFSWSLLGFQKSLEWSERNEEFQKRAGFANMAALAVHAK
                     GLSHEELQLLLDAVIRESHDDRNFARKAVNWALRQIGKRDLRCNGMAIDAAERILAKG
                     "
BASE COUNT          533 a          704 c          860 g          553 t
ORIGIN      
        1 agaaggaggg gatcaagccc atccattaca tcctgatcac cgatctggga ctaaagaagg
       61 tcgacgatct tcaccattcg ctggaggacg tggaccgggc ggcgcgcctt tgccttgacg
      121 ctctgcccag gaactgaccc tttactctta ttttcacccc ctttttccta tttttgattc
      181 catacggaat tttatgagtt taactataca tttatacact tttcgattgt aaatatactc
      241 atttgttgcg tatagaaagt atttattagt taattatcta ttgaggaggc gaagtgaccg
      301 attaaatctc ggtcataata gacaaagagg atgctaaatg atgaaacacg gaatgggtac
      361 gaagttcgcg ctcatcttgg ccttggctct ggtggcgctt cccctgttct cttccccggt
      421 agcggcggcg gacggcgatg tcgacagtgg ggacaccgcc tggctgatga tctccacagg
      481 actggtgttc gtgatgacgc ccgcggtggc cttcttctac ggaggcatgc tgcgcaagag
      541 caacttcctg tccatgctgg gacagagcat actgatcata ggggtggtga ccatcatctg
      601 ggtggtcttc ggttactcgc tgaccttcgg cgctgacaat ggcggcctga tcggcggggc
      661 tgagtacctg atgctcaaca gcgtgaacct cgctccgaac ctgatcgcgc cgaccgtccc
      721 gcacatcctg ttcatgatgt accaggggat gttcgctatc atcaccgtgg ccctgatcat
      781 cggcggggtt gcggaacgca tgaagctcag ctcgctcatg gtgttcctgt ccatctggac
      841 cgtagcggtc tacatcccgg tggctcactg ggtgtggggc gggggatgga tatatgacct
      901 gggggcgctg gatttcgccg gtggaacggt ggtgcacatc accgccggcg tctcggtgct
      961 ggcggcggcc atggtcctgg gcaagcgcat cagccacgcc gggggcggac aggaagtgcc
     1021 ccacaacata ccgatggtag tgctgggcgg cgccctgctc tggataggct ggttcgggtt
     1081 caacggcgga agcgccctgg cctcgaacgg gctggcggcg aacgcgttgg tcgtaaccca
     1141 catctcggca gcgatggccg cgacggtctg gggcctcatc agctggctgg acaccggaag
     1201 ggtgagcgtg ctcggtctca tctccggtgg agtggccggg ctggtggcca tcaccccggc
     1261 cgcgggattc gtcaacccca caggcgctct cttcataggt ctcggagcgg cggtggtctg
     1321 ctacggaggc atactgctgc gcaagcggct gggattcgat gacgccctgg atgtctgggg
     1381 cgtgcacggt ctgggcggta ccttcggtgc gatagccacg ggtattttcg ccaccaccac
     1441 ggtgaacgcg gcggcgaagg atgggctgct ctacggcggc ggggtcgacc tgctggtggc
     1501 ccagctgatc gctgtggctg cagtgtgggc cttcgccttc ggtgtgacct acgtggtcct
     1561 gaaggcgatg tccaaggtga tgaccctgcg catgaccaaa gatgaggaga ggataggcgc
     1621 ggacatcatc cagcacggcg agagcgccta ctatctgagg tgaggaagac gaagaagata
     1681 gaggctatta tccgcagtga gaccttagac tcggtcaagt gcggcctcga aaagctggga
     1741 atgaacgcca tgaccataac tgacgtgctc ggccggggag agcagaaagg gctggagttc
     1801 acccaccggg ctggcaagta ccgggtggac ctgctcccca aggtaaaagt ggagattgtg
     1861 gtcagggacg aggacgtcag gaaagtgatt gccgtaatca tcgagaaggc caggaccggc
     1921 gagataggcg acgggaagat attcgtctcc ccggtagagg aggtgatcag gatcaggacc
     1981 ggcgaaagca gcaatgggaa ggcctgaatc tctccttgac cttcccttct tatctttttt
     2041 tccacctctt ccgatgaacg ctgaagaggt cctcgcgcgt ttgggatcca tacccgaccg
     2101 tgcgcgtctg gaaggcgtgc ccgggtatgg gatcaaggtc gatcgcgcct aagggtgagc
     2161 ctgccgaaga tgagagcgtt ggccaaagac atagggaagg gtcatcaact ggccctggac
     2221 ctttggcgca ccggggtgca cgaggcgctc atattgtcga gccttttggc tgagtccgag
     2281 aagctcaccg aggagcagac gaaggaaatg gtgaggggct tcgattccag ggacctctgc
     2341 gaccagtgct gctccaacct gttcagctgg tccctcttag gctttcagaa atcacttgaa
     2401 tggtcggaga gaaatgagga gttccaaaaa cgggctggct tcgccaacat ggccgccctc
     2461 gccgtgcacg ccaagggact gagccacgag gaacttcagc tgctcctgga tgcggtgatc
     2521 agggagagcc atgacgaccg caacttcgcg cgcaaggcgg tgaattgggc gttgcggcag
     2581 atcggcaaga gggacctgcg ctgcaacggg atggccatcg acgcggccga gaggatcctg
     2641 gccaagggcg
//
LOCUS       DAOSYN010000185         2617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_30451_length_2617_cov_3.973068, whole genome shotgun sequence.
ACCESSION   DAOSYN010000185 DAOSYN010000000
VERSION     DAOSYN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30451_length_2617_cov_3.973068"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /locus_tag="PLJ11_06725"
     CDS             <1..2167
                     /locus_tag="PLJ11_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO04394.1"
                     /translation="MARESYDYYKELHSLNTSEFERDLTSGIEFFEVPSSSDPRRDYE
                     ELLNIIRNDALYRLPELTEPQRHLLKIALYRMSPLYQLICRNTRNLLRKYSARGMARI
                     RVPERLLEDPIPIDFTPEERDLYNSVETRYVKPFYRDYAKAGLPVHGVGFILTIYRKR
                     AASSWASVTKSFQRRREKLDEALANWGPDSIHLLFGSVALKDIIEQEEVEEGEEIERE
                     GAMIDEDLSKPNQRIDLEMVREVVERERTVVEQLLDELEDLRQKGVDSKRDALISHLK
                     SIMAERRGVLVFSQYKDTVDDIAESMFYEFGSSMAKYHGSGGEVWDGGRFKLVKKEDV
                     KKRIENGDFRLLIATDAASEGLNLQMMDAVVNYDLPWNPMRIEQRIGRIDRVTQTSPT
                     ISIGVLVPRNTIEMDVYSRCVERLGMFKQALGPLQPILVESFIEDLVLGRDDIASGWD
                     DIEDRWATAKEHALLFEEALSAQEFKGGWQERKDAERMALQHLLSEVGYIEDGGVWER
                     AGHRISIEGIRDGCDWMTTLSHDRLFVALLSEMGQMPQELEREGLQYKVIESGGSMAL
                     AVKHSDGSVRLIKDLTDIAGENEEQVGTDWEAAIREVNAGETDRRVSFSRFRKEQSRV
                     REKEWRKSVERDVIHPLLRLSEYNPDLCADTIILDPALTSLFLIYSEERDASKNSFLK
                     TLRSVDTFKKSRGKPPSLEKIIKSAEELTVQGRGFQRDR"
BASE COUNT          740 a          537 c          751 g          589 t
ORIGIN      
        1 gatggccagg gagagctatg attattacaa ggaactgcac tcgctgaaca catcggagtt
       61 cgaaagggac ctgacatcgg gaatagagtt ctttgaggtc ccctcatcaa gtgacccaag
      121 aagagattat gaggaattgc tgaacatcat caggaacgat gccttatacc gcctcccgga
      181 actcacggag ccacaacggc acctcctcaa gatcgcgctc tatcgtatga gccccctata
      241 ccagcttatc tgcaggaaca cccgcaatct gttgcggaaa tatagtgcga gagggatggc
      301 gaggatcagg gtcccggaac gattgctgga ggaccccatc cccatagact tcacaccaga
      361 ggagcgggac ctctataatt cggtggagac cagatacgtc aaaccattct acagggacta
      421 tgctaaagct ggcctgcctg tgcatggagt gggtttcatc cttacaatat acagaaagag
      481 ggccgcctca agctgggctt ccgtgaccaa gagcttccag aggaggaggg agaaactgga
      541 tgaggcgttg gctaattggg gaccggacag catccatttg ctcttcggct cagtggccct
      601 gaaggacatc attgaacagg aagaagtgga agaaggagag gagatcgaaa gagagggggc
      661 gatgatcgat gaggatctct cgaagcctaa tcaacgtatc gacctggaaa tggtaagaga
      721 ggtggtagaa agggagagga ctgtcgtcga acagcttctt gatgagctag aagaccttcg
      781 gcagaagggt gtggactcca agagggacgc gttgatatcg catctaaaat cgataatggc
      841 cgaacgcagg ggggtcctgg tgttcagtca gtacaaggat accgtggacg acatagctga
      901 atccatgttc tatgagttcg gttcaagcat ggccaagtac catgggagcg gaggggaggt
      961 gtgggacgga ggacgattca aattggtgaa aaaggaggac gttaaaaaga gaatcgagaa
     1021 tggggatttc cgtcttctca tcgctaccga tgccgcctcg gaaggattga acttacaaat
     1081 gatggacgcg gtggtcaatt atgatctccc ttggaatccc atgagaattg aacagaggat
     1141 cgggaggata gaccgtgtca cccaaacctc cccgaccata tccattggtg tactagttcc
     1201 taggaacacc attgagatgg acgtctattc gaggtgtgtc gaacgtttag gtatgttcaa
     1261 acaagccctt ggcccgttac aaccgatatt ggtggagagc ttcatcgagg atttggtact
     1321 gggaagggat gacatcgcgt cgggatggga tgatatcgag gacagatggg cgaccgcgaa
     1381 ggagcatgcc ctgctcttcg aggaggccct ttctgcacag gaattcaaag gaggatggca
     1441 ggagcgtaag gacgccgagc ggatggcatt acagcatttg ttatctgaag ttggttacat
     1501 cgaggatggt ggagtatggg agagagcagg acatcgcata tcaattgaag gaatcagaga
     1561 tggttgcgat tggatgacca ccctgtccca cgatcgattg ttcgtggcat tattatccga
     1621 gatgggacag atgccccagg aattagagcg ggaaggcctt caatacaaag tgatcgaatc
     1681 agggggttcg atggccttgg cggtcaagca ttcggacgga tcggtccgtt tgatcaaaga
     1741 ccttacagac atagcagggg aaaacgagga acaggtcggg acggattggg aggcggcaat
     1801 acgagaggtg aatgctggtg agaccgaccg cagagtatcg ttcagtagat tcaggaagga
     1861 gcagagcagg gtcagggaga aggagtggag aaaatccgtg gaaagggacg tcatccatcc
     1921 cctccttaga ttgagtgaat acaatcctga cctttgtgct gataccatca tcctggatcc
     1981 ggccctaacc tctctattcc tgatatacag tgaagagagg gatgcatcca agaattcctt
     2041 tttgaagact ttaaggtctg ttgatacatt taagaagagc agagggaagc ctccttcatt
     2101 ggaaaagatc atcaagagtg cggaagagtt gacagttcaa ggtagaggat ttcaacgaga
     2161 ccgttaatcg tcgatgtttg atttgataca attgcgacga actgcgcacg ctcgcagatg
     2221 tgatatgtga tacgatcacg atctggctgt tgatatcgag gtattacgtg ccctatgcac
     2281 ttctatatcc atattacctg agttcgacag ttgaccgagc gaaattacac tggttttatg
     2341 atttgaccct aaacccgggt agtttgatac gtgacttcga cacttgatcg tatcaaaatt
     2401 ggggatgttt gatcattgta ttaatgctgg acgcgagagc gtccataatt gaaaacatta
     2461 atcctgactt cgacacttga tcgtatcaaa attgaggaag gttgatcatt gcattaatgc
     2521 tggacgcgag agcgtccaga aaaaattggt agaaattaaa aaggtcctca acttatgcct
     2581 gaattgtgaa taaacaaaca aagctgagga cgaatca
//
LOCUS       DAOSYN010000186         2605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_30642_length_2605_cov_4.463137, whole genome shotgun sequence.
ACCESSION   DAOSYN010000186 DAOSYN010000000
VERSION     DAOSYN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30642_length_2605_cov_4.463137"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..776
                     /locus_tag="PLJ11_06730"
     CDS             102..776
                     /locus_tag="PLJ11_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HOO04395.1"
                     /translation="MQSENDEKFVTALRRIALMGGLHDYVALSSRELGEMLDMSQQSA
                     SKRILELLDAKLIERDLGARRQRIRITSKGADMLRKEYLEYQKIFEMKDELVIKGTVV
                     EGMGEGQYYVTQPGYMEQFVEKLGFRPYEGTLNVKLLPTEVHKLDALRNGRTIDIKGF
                     ERNGRTFGDVHCIPASIQNIECAVVLPRRSHYEDVLEVVCKFHLRRTLGLEDGDQVEV
                     RIHLSF"
     gene            complement(777..1592)
                     /gene="purQ"
                     /locus_tag="PLJ11_06735"
     CDS             complement(777..1592)
                     /gene="purQ"
                     /locus_tag="PLJ11_06735"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOO04396.1"
                     /translation="MKAVRVCVLRIEGTNCEDEAAQAFASVGASPEKVHLKQLLGQCP
                     SDRRRELDSYDVLMLPGGFSAGDYVRAGAIFAARLKSGLRKELVAFVEAGRPVIGVCN
                     GFQVLVELGLLPAMDGTMAEAPEAALYTNDSGRFECRPTLLRQERRGRGPFTAGIPHG
                     SVVMCPSAHAEGKLMFPRGTRRRTLERLLENDQIVFRYVNPDGEYAGYPWCPNGSIYN
                     IAGICNPAGNVLGMMPHPERVLRRETHPDWTRTPGREEGDGLPLFRSIVESVR"
     gene            complement(1597..1779)
                     /locus_tag="PLJ11_06740"
     CDS             complement(1597..1779)
                     /locus_tag="PLJ11_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04397.1"
                     /translation="MVEVEPSREGKFVSAMTVPITRLGTVGGRSLRLIAGKGRASLSL
                     RDMRKAWSGTLPRLMG"
     gene            complement(1731..>2605)
                     /locus_tag="PLJ11_06745"
     CDS             complement(1731..>2605)
                     /locus_tag="PLJ11_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOO04398.1"
                     /translation="VTLGLLADPNIASKEWVFRMYDHEVRGGTVIKPASGRMNRGGPS
                     DATVIRPLPDRERGLALAVGVNPWFCGADAYRGGMASIDEACRNIIAVGGRPDSFTDC
                     LNFGNPERPERLGELRQAVAGMGDLARYLGLPVPSGNVSLYNETASGAVLPTPTVLGV
                     GIVEDVRRCVTTDLKREGNALYLVGETRRELGGSALYRRFGGRGGEVPAVSPERLRRS
                     MDEMLSAMGEGLIASCHDVSDGGLAIAVAEMCLGATWAPPWRWAPNGRPFRRATPAGW
                     WRSSPPEKESSSPP"
BASE COUNT          445 a          918 c          819 g          423 t
ORIGIN      
        1 aacctgcgga aaggttcagc aatctattaa ttgggataac tgataaccac gaaagtgccc
       61 tgttacaggt tgcatagtag cgggccgggg agttgttgcc tttgcagtca gagaacgacg
      121 agaagttcgt taccgccctc cgcaggatag ccctgatggg ggggttgcac gattacgtcg
      181 ccctttcgtc ccgggagctg ggggaaatgc tcgacatgtc ccagcaatcg gcctccaagc
      241 gcatcctgga gctcctggac gcgaagctca tagaacggga cctgggggcc aggcgccagc
      301 gcatccggat caccagcaag ggggcggaca tgctccgcaa ggagtacctc gagtaccaga
      361 agatcttcga gatgaaggac gagctggtga tcaaggggac ggtggtcgag ggcatgggcg
      421 aggggcagta ctacgtcacg cagcccggct acatggagca gttcgtggag aagctcgggt
      481 tccggcccta cgagggaacg ctgaacgtca agctccttcc gaccgaggtc cacaagctcg
      541 acgccctccg gaacggaagg accatcgaca tcaaggggtt cgagcggaac gggaggacgt
      601 tcggggacgt gcactgcatc ccagccagca ttcagaacat cgaatgcgcg gtcgtcctcc
      661 cccggcgctc gcactacgag gacgtgctgg aggtggtctg caagttccat ctccggcgga
      721 ccctcggcct cgaggacggg gaccaggtgg aggtgcgcat ccacctctcc ttctgatcag
      781 cgcacgcttt ccacgatgga gcggaaaagg ggcaggccgt cgccctcctc cctccccggc
      841 gtcctggtcc agtcgggatg cgtctccctt ctcagcaccc tctccgggtg cggcatcatc
      901 cccaggacgt tcccggccgg gttgcagatc ccggcgatgt tgtagatgga gccgttcggg
      961 caccacgggt agccagcata ctccccgtcc gggttcacgt aacggaacac gatctggtcg
     1021 ttctccagca accgctcgag cgtccgccgc cgcgttcccc gtgggaacat gagcttgccc
     1081 tcagcatggg cggaggggca catcaccact gagccgtgcg ggatgccggc ggtgaagggg
     1141 cccctgcccc gcctctcctg ccggaggagg gtcggccggc actcgaacct gccggagtcg
     1201 ttggtgtaca gcgccgcctc gggcgcctcg gccatggtcc cgtccatcgc cgggagcagc
     1261 cccagctcca ccaggacctg gaagccgttg cacacgccga tcaccggcct tccggcctcc
     1321 acgaaggcga cgagctcctt cctgagcccg ctcttcaggc gggcggcgaa gatcgccccc
     1381 gcccgcacgt aatcgccggc cgagaagccc ccgggcagca ttaggacgtc atagctgtcc
     1441 agctccctcc tccgatcgga ggggcactgg cccaggagct gcttcagatg gaccttctcc
     1501 ggggaggcgc cgacggaggc gaaggcctgg gcggcctcgt cctcgcagtt cgtcccctct
     1561 atcctcagca cacagaccct gacggccttc atcgcatcac cccatcagtc gcgggagcgt
     1621 gccgctccag gcctttctca tgtccctcag ggagagggac gccctcccct tcccggcgat
     1681 cagtcgcaag gaacggccgc cgaccgtccc gagccgggtg atgggaacgg tcatggcgga
     1741 gacgaacttt ccttctctgg agggctcgac ctccaccacc cagcgggtgt tgctctccga
     1801 aaaggcctcc cattcggcgc ccacctccac ggcggcgccc aggtcgcgcc caggcacatc
     1861 tcggccaccg cgatcgccag tcccccatcg gagacatcgt ggcaggaggc gatcagcccc
     1921 tcccccatcg cggagagcat ctcgtccatg gagcgtctca gccgctccgg ggacacggcc
     1981 gggacctcgc ccccccttcc cccgaacctg cggtagaggg cagagccccc gagctcccgc
     2041 ctggtctcgc ctacgaggta cagggcgttc ccttccctct tcaggtcggt ggtcacgcac
     2101 ctgcgcacat cctccactat ccccacgccg agcacggtgg gagtggggag cacggccccg
     2161 ctggccgtct cgttgtagag cgagacgttg ccggacggca ccggcaggcc caggtacctg
     2221 gccaggtcac ccatgcccgc cacggcctgc cgtagctccc ctagccgctc aggtctctcc
     2281 gggttcccga agttgaggca gtccgtgaac gaatcagggc gcccgcccac ggcgatgatg
     2341 ttacggcacg cctcgtcgat ggaggccatc ccgccgcggt acgcatccgc cccgcagaac
     2401 cagggattga cgccgaccgc cagggccagc cccctctccc ggtccggaag gggcctgatg
     2461 accgtcgcgt ccgaggggcc gcccctgttc atccttcccg aggccggttt gatgacggtg
     2521 ccgccgcgca cctcgtggtc gtacatgcgg aacacccact ccttgctggc gatgttgggg
     2581 tccgccagca gccccaacgt cacgg
//
LOCUS       DAOSYN010000187         2604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_30666_length_2604_cov_2.577481, whole genome shotgun sequence.
ACCESSION   DAOSYN010000187 DAOSYN010000000
VERSION     DAOSYN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30666_length_2604_cov_2.577481"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..502)
                     /locus_tag="PLJ11_06750"
     CDS             complement(215..502)
                     /locus_tag="PLJ11_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO04399.1"
                     /translation="MSTPSGYTIEHDIKGEARKFRCGSKELREFRNDRRKAAVRLALE
                     RGVGKVVELIRCSARSVRNWLIRWRRGGEAALDEKSRRPKNVRYLEREKVD"
     gene            766..>2604
                     /locus_tag="PLJ11_06755"
     CDS             766..>2604
                     /locus_tag="PLJ11_06755"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOO04400.1"
                     /translation="MKGVIEVCKPREDIIKGDVSDEIYAAHLGQVRDERAPSVYTDPN
                     TFFRNTYPTDGLKTTIREVFSRLSGSSKSGSPLIKLETSLGGGKTHTLIALYHLSKYG
                     SRCPGADRFIGDMDFGPVNVSVTIGTEMSVKQTGSGPRTLWGHIAQNLFGDSGYQKVL
                     EEDREMFSPDELTLRNLFGDERCLILIDELALYLAKAVTVDVKGSNLAKQTTVFLQTL
                     TEVAASCRNVVVVLTSLSKDSVFKEESEEILRHLDSDIKKERAKDAIGDAEKIVSRMV
                     MTLTPTKGEEFSAVVRHRLFESVDMEEAESVIQAYMAYLSSEGNVDYLPQHARGKKYL
                     EDLRSAYPFHPELINILRTKTSSVPNFNKTRGVLRLLSKVVRNIWNDGRNVLLIHPYE
                     IDFRKQDFVEEVVSRLDKGEYQSALAADISNQREPPRSARVDEQFSEPLGTMISNVVF
                     LNSITGAVGTDIEHGVKEVEIYLSMARPGFDLKMAKDALRLLEDNCFYMVRQGSKFAY
                     QTEPNLVKLIEDAKDTVERTRAREEVRDRIKNLYSGKKYFQPIFFPNEPVKVPDDTER
                     TKLCVMDFDDCSIKGKSPKVPPDVRSIYEQAGSQKVPRIFANNLLFL"
BASE COUNT          613 a          712 c          701 g          578 t
ORIGIN      
        1 ggtatcggtt ctggagcgca tcgggtacca tcacgtccat ctgccacagg gagttcgaat
       61 gcttccgctc gaaggagcgg aagcgacgcc tccccgggct ctggcggttg agatgatgag
      121 catccagata tctatgcacg gtgctcgagg agcaccgcac gtcgaaggag atcttcgagc
      181 acccgtaacc ctgtctttta cgaatgtcca gtatctagtc gaccttctcc ctttccagat
      241 aacgaacgtt cttcggccgt ctgctctttt cgtccagggc ggcctcaccg ccccttctcc
      301 atcgtataag ccagttcctg acggacctgg cggagcatcg tatgagctcc acgacctttc
      361 cgacgcctct ctccaatgcc agtctgaccg ccgccttgcg gcggtcgttg cggaactcgc
      421 gcaactcctt gcttccgcaa cggaactttc tcgcctcacc tttaatatcg tgctcgatcg
      481 tatacccact gggtgtgctc attttttctg tctcctaaac aaaagaatga acacacccac
      541 ccttactgat ttctgagctt tttcaggacc tcttccccaa gctcatctgg accagcttcg
      601 gaaagatgtc ttcgaacagc tcactcatat atttaaattc ggtcatatct ataatcatat
      661 tgtattatgt aacttttttc ttatcgatca atcttcgttt cgaaaatcaa gggcgaatag
      721 cataataact gaagggtatt cattcccatc tcagagggat tggaaatgaa aggggtcatt
      781 gaggtctgca aaccgcgaga ggacataatc aaaggggacg tttcagacga gatatatgca
      841 gcgcatctgg gtcaagtaag ggacgaaagg gctcctagtg tctacactga cccgaacact
      901 ttcttccgaa acacctatcc caccgatggg ttaaagacca ccatcaggga ggtgttctcc
      961 cgcttgtcag gctccagcaa gagcggttcc cctcttatta aactggaaac ttctttgggt
     1021 ggcggcaaga cccacacact gatcgcttta taccatcttt ccaagtacgg ctccagatgt
     1081 ccaggcgcgg acaggttcat cggtgacatg gatttcggtc cggtgaacgt ctcggtcacc
     1141 atagggacgg agatgagcgt gaagcagaca gggagtggac cgaggacgct atgggggcac
     1201 atagcccaga acctgttcgg tgacagcggc tatcagaagg tgttggaaga ggaccgggag
     1261 atgttctctc ccgatgagct caccctgcgc aaccttttcg gagatgaacg ttgcctgata
     1321 ttgatcgatg agctggcatt gtacttggcg aaggcggtga ccgtagatgt aaagggcagc
     1381 aatctagcca agcagacgac agtgttcctt cagaccctta ccgaagtggc cgcctcctgc
     1441 aggaacgttg tcgtggtcct taccagcttg agcaaggact cggtgttcaa ggaggaatcc
     1501 gaggagatac tcaggcatct ggacagcgac atcaagaagg agagggccaa ggacgccatc
     1561 ggtgatgctg agaagatcgt ttcccgcatg gtgatgacct tgaccccgac caaaggcgag
     1621 gagttctcgg cggtggtacg ccatcgtctc ttcgaatctg tggacatgga ggaggcagag
     1681 tcggtcatcc aggcatatat ggcctacctt tccagcgaag gcaacgtcga ttatctcccc
     1741 cagcatgccc gaggaaagaa gtaccttgag gacctgcggt ccgcctatcc tttccatccc
     1801 gaacttatca atatcctccg gacgaagacg agttccgtgc ccaacttcaa caagacccgc
     1861 ggcgtgctca gattgctaag caaggtcgtg cgcaatattt ggaacgatgg gaggaacgtt
     1921 cttctcatcc atccttacga gatcgacttc cgcaagcagg acttcgtgga ggaggtggtc
     1981 tcccgattgg acaagggcga gtaccagtca gcgttggccg ccgacatatc caaccagcgg
     2041 gaaccaccca ggtcggcaag ggtggacgag cagttctccg agcctctagg gaccatgata
     2101 agcaatgtgg tcttcctcaa cagcatcacc ggagcggtgg gaacggacat cgagcacggg
     2161 gtgaaggagg tggagatcta cctctccatg gcccgtccag ggttcgacct gaagatggcc
     2221 aaggacgcgc ttaggctcct ggaggacaac tgcttctata tggtacggca gggctctaag
     2281 ttcgcctacc agaccgagcc taaccttgtc aagcttatcg aggacgccaa ggacaccgtg
     2341 gagaggacca gggccaggga ggaggtgcgc gacaggatca agaaccttta ctcgggcaag
     2401 aagtacttcc agcccatctt cttcccgaac gagccggtca aggtccccga cgataccgag
     2461 aggaccaagt tgtgtgtcat ggacttcgat gactgctcta tcaagggcaa gagcccgaag
     2521 gtgccacccg atgtgcgttc gatctatgaa caggccggtt cgcagaaggt cccacggata
     2581 tttgcgaaca acctcctctt cctg
//
LOCUS       DAOSYN010000188         2599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_30753_length_2599_cov_2.743711, whole genome shotgun sequence.
ACCESSION   DAOSYN010000188 DAOSYN010000000
VERSION     DAOSYN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30753_length_2599_cov_2.743711"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLJ11_06760"
     CDS             <1..286
                     /locus_tag="PLJ11_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176763.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOO04401.1"
                     /translation="TMFDLGRDVPLKDIIQKAKDENAQIIATSTLMTPTLAGMKEIER
                     MLVESGLKGKIKTMIGGGATSQDFAKSIGADGWGYDANEAVKVAVELLKK"
     gene            422..1216
                     /locus_tag="PLJ11_06765"
     CDS             422..1216
                     /locus_tag="PLJ11_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOO04402.1"
                     /translation="MSDMERELFQLRQRVDELGMELRYISTLLEGSQLGINGRSEVFE
                     LLRDEAREKAESRLTAGMTKRCEMRPECRRRFMELLDENLDMMGQRRISEEDMCRQSQ
                     KLETLRSTAVPGRCDACFKEVGALFKDQTELMRQLRLYMSRDEVRESIEALPEEAVVR
                     GLLEPMANVQRLVIMKALSRSQRSFSELSNLTNLRGGNLLFHLQRLTSSGMVFQRGGR
                     GDYALTPKGHMALELINDLYLRTMAGGEGSNIHTPLVDQGVVGKVE"
     gene            1271..>2599
                     /locus_tag="PLJ11_06770"
     CDS             1271..>2599
                     /locus_tag="PLJ11_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04403.1"
                     /translation="MRGITSRTLLGTLLSWTIIFEGLFALSIAAQASIEGLGTILAST
                     FTLAAAQLTSLGIFIGTMWTLKIAFPDPRRTVLVRLFGLLTYLAMVMLAMEGLVMVLI
                     AGNVNVEGFGGVGKKWIVLIGAQLFAIAVLSLRLWRLRDIRPVNWLADTLGSLASAML
                     MLEGLTAIGIAGNATITGLGTVLERTMVYAGAGLFLLGALIFLVWTITSDPWAAPRLQ
                     KRIGLGWALPVMGVLGGLVAAAAVAVSTVAGSVTVAGAGSATKGYVVAGIAQLFALGL
                     VSPILWKLSLERLDRRSLVQFIAAASMAMLAFEGVFAIALAANTHIEGIGGILEGTFR
                     LAGAQLLALSVVALSMWIIRDSPRLGGRAKLVVSALSLLSLTVIALEGLAVMVLAGNL
                     RIDDLGSVRERYVLMGGAQMALLASLALLCHARPEGVRALKVAGTAAAFFS"
BASE COUNT          513 a          727 c          842 g          517 t
ORIGIN      
        1 caccatgttc gacctgggcc gtgacgtgcc cctgaaggac atcatacaga aggccaagga
       61 cgagaacgcc cagatcattg cgacctccac tctgatgact ccgaccctgg cagggatgaa
      121 ggagatagag aggatgctgg tcgagagcgg cctgaagggc aagataaaga ccatgatcgg
      181 cggcggtgct acctcccagg actttgctaa gagcatcggg gccgatggct ggggctacga
      241 cgcgaacgag gccgtcaagg tagccgtcga actcttgaag aagtaggggg cttcgccccc
      301 gatctctttt tttattcaaa aatattaaaa taagttatta tgaatatatt taatggtaca
      361 ttattttata ctccgagggt atcattatgt agcgataatg atgcaaaata cgagcactga
      421 gatgagcgat atggaaaggg agctgttcca gctgaggcag agggtggatg agctggggat
      481 ggagctccgg tacatcagca cccttctgga aggctcgcag ctcggcatca acggccggtc
      541 tgaggtgttc gagctgttgc gcgatgaggc gcgcgaaaag gcggagagcc ggctcacggc
      601 cggaatgaca aagcgatgcg aaatgcgccc cgagtgccgg cggaggttca tggagctgct
      661 cgatgagaac ctggacatga tggggcagcg gaggatctcc gaggaggaca tgtgcaggca
      721 atcgcagaag cttgaaacgc tccgttccac tgccgtccct ggcaggtgcg atgcatgctt
      781 caaggaggtc ggcgcccttt tcaaggacca gacggagctg atgcgccagc ttaggctcta
      841 tatgagccgg gatgaggtca gagagagcat cgaggccctg ccggaggagg cggtggtgcg
      901 ggggctgttg gagccaatgg ccaacgtcca aaggctggtc atcatgaagg cattgagcag
      961 gtcccagcgc tccttctccg agctctcgaa cctaaccaac cttcggggcg gcaacctact
     1021 gttccatctc caaaggctga ccagctcggg catggtcttc cagaggggag gccggggtga
     1081 ctacgcccta acacccaagg ggcatatggc gctagagctg atcaacgatc tatacctccg
     1141 gacgatggcg ggcggggaag ggtccaacat ccacacccct ctcgtcgacc agggggtggt
     1201 tgggaaggtt gaatgattgc acaagttgta taaatcagga gattggtaac tgggagggta
     1261 gggggaagag atgaggggaa tcaccagccg aacattgctc ggaacgcttt tgagctggac
     1321 catcatcttc gaggggctct tcgccctcag tatcgctgct caagcaagca tagagggcct
     1381 cggtaccatc ctcgcctcca cattcacact agcggccgct caactcacgt ccctcggcat
     1441 tttcattggg accatgtgga ccttgaagat cgctttccct gacccgaggc gaacggtgct
     1501 ggtcaggctg ttcggcctcc tcacctatct ggccatggtc atgttggcca tggaggggtt
     1561 ggtcatggtc ctcatcgccg ggaacgtgaa cgtggaaggc ttcgggggcg ttggaaagaa
     1621 atggatcgtc ctgatagggg cgcagctctt cgccatcgcc gtcctctccc tgaggctgtg
     1681 gcgcctaagg gacatccgcc cggtcaattg gctcgcggac accctgggct cgctggcctc
     1741 cgccatgctt atgctggagg gcctaaccgc aatagggatc gcgggcaacg ccaccatcac
     1801 tggcctcgga acggtcctgg aaaggacgat ggtgtatgcc ggagcagggc tcttcctgct
     1861 cggggccctc atctttctcg tttggacgat caccagcgat ccctgggctg ctccccgcct
     1921 tcaaaagcgc atcgggctgg gttgggcctt gcccgtcatg ggcgtgctcg ggggcttggt
     1981 cgccgccgcc gcggtcgccg tctccactgt ggcaggctcg gtgaccgttg ccggcgccgg
     2041 cagcgccacc aaagggtacg ttgtagcggg catcgcccag ctgttcgccc tggggctggt
     2101 ctcacccatc ttatggaagc tcagccttga gaggctcgac cgccggtccc tggtccaatt
     2161 catagcggcc gcctcgatgg cgatgctggc cttcgagggc gtcttcgcaa tagccctcgc
     2221 ggcgaacacc cacatcgagg gcatcggagg gatattggag ggaaccttcc ggcttgccgg
     2281 agcgcagctg ctcgccctct cagtggtagc cttgtccatg tggatcattc gagacagccc
     2341 gaggctggga ggaagggcaa agctcgtcgt ctccgcgctc tccctccttt cgctgacggt
     2401 gatcgccctc gagggactgg cggtaatggt cctggccggg aacctccgca tcgacgatct
     2461 cgggagcgtc agggaaaggt acgttctgat ggggggtgcg caaatggcgc tcctggcgtc
     2521 gctggcgctg ctctgccatg ctaggccgga gggcgtgagg gcgctcaagg tggccggtac
     2581 ggccgccgcc ttcttctcc
//
LOCUS       DAOSYN010000189         2590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_30867_length_2590_cov_4.201183, whole genome shotgun sequence.
ACCESSION   DAOSYN010000189 DAOSYN010000000
VERSION     DAOSYN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30867_length_2590_cov_4.201183"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLJ11_06775"
     CDS             <1..317
                     /locus_tag="PLJ11_06775"
                     /inference="COORDINATES: protein motif:HMM:NF039705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HOO04404.1"
                     /translation="IISSLERGGARVTRALAKDVTAIWAKRFDKVHVKVSINDVHKTI
                     PAEVDALPEEEFFIGDLMTIGRYDVVITQIKTRDAMARRGAVMARDIVRIYAKRARTT
                     CS"
     gene            286..969
                     /locus_tag="PLJ11_06780"
     CDS             286..969
                     /locus_tag="PLJ11_06780"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF010549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOO04405.1"
                     /translation="MPSGPGPPARDAQEGERRRMVADLKARGAIRSPQVEAAMLRVPR
                     HLFVPERCRPLAYRDSPLPIDAGQTISAPHMVAVMAEALDLRPGQKVLEVGTGSGYNA
                     AVMAELVGDGKVVTIERHPSLAEGARKALQEAGYDNVQVVVGDGSLGLEAEAPYDRIS
                     VTCGAPEVPGPLLEQLKEGGTMVVPVGGLTYQSLLRVRKREGRVETDDLGPVVFVPLL
                     GEKGHHRSQ"
     gene            complement(961..1617)
                     /locus_tag="PLJ11_06785"
     CDS             complement(961..1617)
                     /locus_tag="PLJ11_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOO04406.1"
                     /translation="MRENGRRFDLVAFDMDGVLVDYLSSWTWVHDRFGVSNEEAVVSY
                     MDGRIDDLEFMRRDIGLWLKHRPGLGIPDVERELAMLPINPGIESTVRTLHARGTRAV
                     IISGGLDLIAAKLAERYGFDAQWSNGLAADEGGRLTGEGVLRVELCNKRKALEEAQGM
                     FGASPERTAAVGNSAIDVSMFKGAALGIAFNPIDEDVVRGADHVVRAASLSAILPFLL
                     "
     gene            1671..2369
                     /locus_tag="PLJ11_06790"
     CDS             1671..2369
                     /locus_tag="PLJ11_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOO04407.1"
                     /translation="MIVLVGVAHVIDVSEQIGALVAHHSPGAVGVELDPGRYRALREK
                     GEHGNAPLPYRLLAFMQGRLAEQFGGEVGAEMLAAVEAARRSGAQVLFLDMEAGKVFA
                     AMLAQMPLKEKVLMALSALPGLFMGRERVEKELERFQEDEEAYLGSMAKNYPTVKRVL
                     IDERNEHMAREILMAEGTHGSVLAVVGDGHVEGIRRLLARDDLIVYRLRDLKDLRLDA
                     PAGTAEASFSFFLE"
     gene            2408..>2590
                     /locus_tag="PLJ11_06795"
     CDS             2408..>2590
                     /locus_tag="PLJ11_06795"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOO04408.1"
                     /translation="MKVVLLSYTRDAERLCAAAARSCYSPDPASKLLEEVEEGKAGRL
                     IDQVVERGHHSVIEHAS"
BASE COUNT          432 a          849 c          913 g          396 t
ORIGIN      
        1 tcatcatcag ctcgttggag aggggagggg ccagggtgac cagggccctg gccaaggacg
       61 tcacggccat ctgggccaag cgcttcgaca aggtgcatgt gaaagtctcc atcaacgacg
      121 tgcacaagac catccccgcg gaggtggacg ccctgccgga ggaggagttc ttcatagggg
      181 acctgatgac catcggacgg tatgacgtcg tgatcaccca gataaagacc agggacgcca
      241 tggcccggcg cggcgccgtg atggcccggg acatcgtcag gatctatgcc aagcgggccc
      301 ggaccacctg ctcgtgatgc ccaggagggg gagaggcggc ggatggtcgc tgacctcaag
      361 gcccgggggg ccatccgctc cccgcaggtc gaggcggcca tgctgcgcgt cccccgccac
      421 ctgttcgtcc ccgagcgctg ccgccccctg gcctatcgcg acagccccct tccgatcgac
      481 gccggccaga ccatatccgc tccgcacatg gtcgcggtga tggccgaggc cctggacctg
      541 aggccggggc agaaggtcct ggaggtcggc acaggatcgg ggtacaacgc cgcggtgatg
      601 gccgagcttg tgggggacgg aaaggtcgtg acgatcgagc gccacccgtc gctggcggaa
      661 ggggcgagga aggcgctgca ggaggccggc tatgataacg tccaggtcgt ggtaggggat
      721 ggctccctgg ggctcgaggc ggaggccccc tacgaccgga tcagcgtgac ctgcggggcg
      781 cccgaggtcc ccgggccgct cctcgagcag cttaaggagg gcgggacgat ggtggtcccc
      841 gtgggagggc tcacctacca atccctgctg agggtgagga agcgcgaggg gcgcgtggag
      901 accgacgacc tggggccggt ggtcttcgtc cccctcctgg gggagaaggg gcaccaccgc
      961 tcacaatagg aacggaagga tggcgctgag ggaggcggcg cgcaccacat ggtccgcgcc
     1021 cctgaccacg tcctcgtcga tcgggttgaa ggcgatgccc agggcggcgc ccttgaacat
     1081 cgagacgtcg atggcgctgt tgcccaccgc cgcggtgcgc tccggcgagg ccccgaacat
     1141 cccctgggcc tcctccagcg ccttgcgctt gttgcacagc tccacccgca ggaccccctc
     1201 ccccgtgagc ctgcccccct cgtcggcggc cagcccgttc gaccactggg cgtcgaagcc
     1261 gtaccgctcg gccagcttcg cggcgatgag gtccagcccg ccgctgatga tgaccgccct
     1321 ggtcccccgg gcgtgcaggg tccggacagt gctttcgatg ccgggattga tggggagcat
     1381 tgccagctcc ctctccacgt cagggatccc gagcccgggg cgatgcttga gccagagccc
     1441 gatgtcccgg cgcatgaact ccaggtcgtc gatcctaccg tccatgtagg acaccaccgc
     1501 ctcctcgttg ctcaccccga agcggtcatg cacccacgtc caggacgaga ggtaatcgac
     1561 cagcaccccg tccatgtcaa aggccaccag gtcgaaccgg cgcccgttct ctctcatcgt
     1621 ccaaagccag gagggaatca ttataagaag gttgctgcga tcctcggggc atgatcgtgc
     1681 tggtgggggt ggcccatgtc atcgacgtct ccgagcagat aggcgcgctg gtcgcccatc
     1741 attcccccgg ggcggtgggt gtggagctgg acccaggccg ctaccgcgcc ctgcgggaga
     1801 agggggagca cgggaacgcc cctctcccgt accggctcct ggcgttcatg caggggcgcc
     1861 tggccgagca gttcggcggg gaggtggggg cggagatgct cgcggccgtc gaggccgccc
     1921 gccggagcgg ggcgcaggtc ctgttcctgg acatggaggc cggaaaggtc ttcgccgcga
     1981 tgctggcgca gatgcctctc aaggagaagg tgctcatggc gctctccgcc ctgcccggcc
     2041 ttttcatggg cagggaacgg gtggagaagg agctcgaaag gttccaggag gacgaggagg
     2101 cctacctggg gtccatggcc aagaactatc ccacggtcaa gcgggtgctc atcgacgagc
     2161 ggaatgagca catggcgagg gagatcctca tggccgaggg gacccacggc agcgtgctgg
     2221 cggtggtggg ggacgggcac gtcgagggca tccgcaggct cctggcccgc gatgacctga
     2281 tcgtctatcg gctgagggac ctcaaggacc tgaggctgga tgccccggcg ggcaccgccg
     2341 aggcgtcctt ctccttcttc ctggaatagg gcaaaccctt ttccccggca tcgcataccc
     2401 ttcatcgatg aaggtcgtcc tgctctccta caccagggac gcggagcgcc tctgcgccgc
     2461 ggccgcccgc tcctgctact ccccggaccc ggcgagcaag ctcctggagg aggtggagga
     2521 gggcaaggcc ggcaggctca tcgaccaggt ggtggagagg gggcatcatt ccgtcatcga
     2581 gcacgcctcc
//
LOCUS       DAOSYN010000190         2573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_31103_length_2573_cov_2.964654, whole genome shotgun sequence.
ACCESSION   DAOSYN010000190 DAOSYN010000000
VERSION     DAOSYN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31103_length_2573_cov_2.964654"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1771
                     /locus_tag="PLJ11_06800"
     CDS             425..1771
                     /locus_tag="PLJ11_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOO04409.1"
                     /translation="MAEAPRVVIAGERSGTGKSTVTIGLLMALRRKGLRVQPFKVGPD
                     FLDPMHHTRAAGRPSRNLDTWMFPSYVPQAFANGMRGADMGVIEGVMGMHDGLDGRSE
                     EGSTAHLAKTLRAPVVLVLDARASARSLGAVALGLQAYDPEVTVAGVIFNNVAGGRHL
                     ETLEDALRGEIPCLGGIPSAEDMGLESRHLGLVPAGEVRDEGRYEAIRRTVEGNVDMD
                     ALRAVAEAAPPLELPPPLPLDAPVRAKVGVAMDEAFNFYYQDNLDLLRAHGAEVVPFS
                     PLRDSLPAVDGLYFGGGYPELFAEGLQANEGMREAVRAFAEDRPVYAECGGLMYACSE
                     LVDLEGRRWRMSGLFDAEVTMAPRLEALGYVEVRMVRDCLLGRAGTVVRGHVFHYSRV
                     TRAGGAFAYDLGKAKGIAGAADGLVRGNALASYAHLHFGTDPALADNLVTACARHP"
     gene            complement(1790..>2573)
                     /gene="gpmA"
                     /locus_tag="PLJ11_06805"
     CDS             complement(1790..>2573)
                     /gene="gpmA"
                     /locus_tag="PLJ11_06805"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOO04410.1"
                     /translation="TVFTTPGDWEGRTMIKVVLLRHGESVWNKENRFTGWTDVGLSER
                     GVEEAHEAARLLQGAGFEFDVAFTSVLKRAIKTLDIVLEDMDQCWLPVHKSWRLNEKH
                     YGALQGLNKGEMARRYGEEQVLLWRRAYDVRPPALDPDDPRHPRHDRRYADLPPEAVP
                     ATECLKDTVARFVPYWEGSIVPELQRGRRTIISAHGNSLRALVKYLDGIPDGEIVALN
                     IPTGVPLVYELDEGMRPLRSYYLGDQDAVRSAMDAVARQGRA"
BASE COUNT          385 a          904 c          853 g          431 t
ORIGIN      
        1 gacgccgatg atgagtctgt caagcttgga gtggtcgatc catccggcgg aggccttcat
       61 gccctccacc ctctccaact gctcccttaa aaccttcccg aaatgctcct tggcggcctc
      121 tatctctgcg tcgttcatca tgaccaggga acggcagccc atcaccgctt attacgtttt
      181 tgcatcgggc gccgtccctt gcgcacatca agaaatgtca gcgtccgggg ggatcggcgg
      241 aggatcgtac gatggttcag ggagagaggt gtgggatggg cccttcttcc ggacgcctta
      301 gcctataagg cgttaaaact attgcacccc ctccctaata aaggcttgcc ctcgcctatc
      361 aaatggcgcc tccgccgctc ggacggatag gtagatatcg ccccatgccc ctgcccaggg
      421 gcgtatggcc gaggcaccga gggtggtcat cgcgggggag cggagcggga ccgggaaatc
      481 gaccgtcacc atcggcctcc tcatggccct ccggcggaag gggctgaggg tccagccctt
      541 caaggtcggt ccggacttcc ttgaccccat gcatcacacc agggccgcgg ggcgcccctc
      601 gcgcaacctc gacacctgga tgttcccctc ctacgtcccc caggccttcg cgaacggcat
      661 gcggggcgcg gacatgggcg tgatcgaggg cgtcatgggg atgcacgatg gcctggacgg
      721 ccgctccgag gagggctcca ccgcgcacct ggccaagacg ctccgggcgc cggtggtgct
      781 ggtcctcgac gcccgtgcct ccgcccgctc gctcggggcg gtggcgctcg gcctccaggc
      841 ctacgaccct gaggtgacgg tggcgggggt catcttcaac aacgtggccg gcggaaggca
      901 tttggagacg ctcgaggacg ccctgcgcgg cgagatccct tgcctggggg gcatcccctc
      961 cgccgaggac atggggctgg agagccggca tctcggcctc gtgccggccg gggaggtgag
     1021 ggacgagggc aggtacgagg cgatccgcag gacggtggag gggaacgttg acatggacgc
     1081 gctgagggcg gtcgccgagg cggccccccc gctcgagctt ccgccccccc tcccccttga
     1141 cgcgccggta agggcgaagg tcggcgtggc gatggacgag gccttcaact tctactacca
     1201 ggacaacctg gacctgctca gggcgcacgg ggcggaggtg gtgcccttct cccccctgcg
     1261 ggacagcctt ccggccgtgg acggcctcta cttcgggggc ggctaccccg agctcttcgc
     1321 ggaggggctg caggccaacg aggggatgag ggaggcagtg cgcgcattcg ccgaggaccg
     1381 cccggtctac gcggaatgcg gcggcctcat gtacgcctgc tccgaactgg tggacctcga
     1441 gggccgccgc tggcggatga gcgggctgtt cgacgcggag gtgacgatgg ccccccgcct
     1501 ggaggcgctc gggtacgtgg aggtgcggat ggtccgggac tgcctcctcg gccgcgcagg
     1561 cacggtcgtc cgggggcacg tgttccatta ctccagggtc accagggccg ggggcgcgtt
     1621 cgcctacgac ctggggaagg ccaagggcat cgccggggcg gccgacggcc tcgtccgggg
     1681 gaacgccctc gcctcctacg cccacctgca cttcgggacc gaccccgcct tggccgataa
     1741 ccttgtcacg gcgtgcgccc ggcacccata acccccatcg ttccccgccc taggccctgc
     1801 cctgtctggc caccgcgtcc atggcgctcc ggaccgcgtc ctggtccccc agatagtagc
     1861 tgcgcagcgg ccgcatcccc tcgtccagct catagacgag gggcacgccc gtggggatgt
     1921 tcagggcgac gatctccccg tccggaatgc cgtccaggta cttcacgagc gcccggaggg
     1981 aattgccgtg cgcggagatg atggtcctcc tcccccgctg gagctcgggg acgatgctcc
     2041 cctcccaata ggggacgaag cgggcgacgg tgtccttcag gcactccgtc gccggcaccg
     2101 cctccggcgg caggtccgcg tacctgcggt catgacgggg atgcctaggg tcgtccgggt
     2161 ccagggcggg cggccgcacg tcgtacgccc ggcgccagag cagcacctgc tcctccccat
     2221 atctccgggc catctccccc ttgttcagcc cctgcagcgc cccgtagtgc ttctcgttga
     2281 gccgccagga cttgtgcact gggagccagc actggtccat gtcctccagc acgatgtcga
     2341 gcgtcttgat cgcccgcttc agcacggagg tgaacgccac gtcgaactcg aagccggccc
     2401 cctgcagcag ccgggcggcc tcgtgggcct cctccacgcc cctctccgag aggccgacgt
     2461 ccgtccagcc ggtgaacctg ttctccttgt tccagacgct ctccccatgc cgcaacagta
     2521 cgaccttgat cattgtcctc ccctcccaat caccgggtgt ggtaaaaaca gtt
//
LOCUS       DAOSYN010000191         2570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_31156_length_2570_cov_2.100596, whole genome shotgun sequence.
ACCESSION   DAOSYN010000191 DAOSYN010000000
VERSION     DAOSYN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31156_length_2570_cov_2.100596"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1167
                     /gene="pylB"
                     /locus_tag="PLJ11_06810"
     CDS             58..1167
                     /gene="pylB"
                     /locus_tag="PLJ11_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylornithine synthase PylB"
                     /protein_id="HOO04411.1"
                     /translation="MNPLTLSEDRWSTPPFELDDILDRALAEEGPNLRETAFLLGLTQ
                     RQDLDRLYDTASKVREIHFSNEIFLYGFVYFSTYCKNRCAFCYYNCKNSVSPRYRKSP
                     EEVLAISTMLERSGVHLIDLTMGEDPYYHGPVGWQRLVDLASKVHDTVGLPLMISPGV
                     LPDRVLFDLTKAGVDWYACYQETYNRQLFRSLRVGQDFDVRLDQRLSAARLGMLVEDG
                     MLLNVGESIGDRAYAIHQMKAYNMHQVRAMTFVPQVGTPLFREDRPTIEDELKMISVM
                     RLVCQDRLIPASLDVEGTDGLKARIAAGANVVTSIIPPEGGLAGVAQHDLDIGNGRRT
                     VPAVTRILETMDMEVASISHYKDYIQKWREGRAVG"
     gene            1507..2361
                     /gene="pylD"
                     /locus_tag="PLJ11_06815"
     CDS             1507..2361
                     /gene="pylD"
                     /locus_tag="PLJ11_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methylornithyl-N6-L-lysine dehydrogenase PylD"
                     /protein_id="HOO04412.1"
                     /translation="MTRLTKEMIEEIEGDLEITEHRLRKAIGMDLRSLALRAVGLEAD
                     ADVSKFSAAIVPVTSGLGVTPGFVSSVATILARLGMEAAVTNATDVAGMAEGIRMGSD
                     MVFMADDNVFMAHPVGTWEVIDNAACTALGFVQALEEAADGLGGKEVVVIGLGRVGSF
                     AVAELLARGARPIGVDIDPVALMRARERFGIPCSSDLKRVMPAADLVVHACPAPVRGE
                     LVKEGAIISAPGVPCGFDAIGYGRAGLIMHDMLPTGVAVMAAQACRALMAANVANYPV
                     EWGLAVHE"
     gene            2354..>2570
                     /locus_tag="PLJ11_06820"
     CDS             2354..>2570
                     /locus_tag="PLJ11_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOO04413.1"
                     /translation="MSELGIALDIGTSGFRAQAIDLATGEVLSTAITVRHPIPGSNVM
                     DHVSFAIEVGEDECNRLMVSTINQLIRR"
BASE COUNT          525 a          698 c          859 g          488 t
ORIGIN      
        1 caacattaag cgagtgggca gaagcctggt ctaccttgat ggggtgaggt tggacatatg
       61 aacccgttaa cattgtcgga ggaccgttgg tcgacccccc ctttcgaact tgacgatatc
      121 ctcgatcggg cattggccga ggaagggcca aacctgaggg agacggcctt cctgcttgga
      181 cttacccaac ggcaggacct ggataggcta tacgatacgg ccagtaaggt tcgggaaatc
      241 catttctcga atgagatatt cctctatggc tttgtctact tcagcaccta ttgcaagaac
      301 agatgcgcct tctgctacta caattgcaag aacagcgtga gcccgcgcta tcgcaaatcg
      361 ccggaggaag tgctcgccat ctcaactatg ctcgaaagga gcggggtcca tctcatagac
      421 cttaccatgg gggaggaccc ctactatcac ggacccgtcg gatggcaaag gctggtagat
      481 ctggccagca aggtccatga taccgttgga ttgccgctga tgatctcgcc cggggtgttg
      541 ccggaccgcg tcctgttcga cctgaccaag gcgggggtgg attggtacgc gtgctaccag
      601 gagacctaca acaggcagtt gttccgaagc ctgcgggtgg ggcaggactt cgatgtaagg
      661 ctggaccagc gcctctcggc cgctaggctg ggcatgctcg ttgaggacgg gatgctgttg
      721 aacgtgggcg aatccatagg ggaccgggcc tacgccatcc atcagatgaa ggcctataac
      781 atgcaccagg tccgggcaat gaccttcgtt cctcaggtcg gcaccccgct gtttagggag
      841 gataggccca ccatagagga tgagctgaag atgatctccg tgatgcgctt ggtatgccag
      901 gacagactga taccggcgag cctggacgtc gaggggacgg atggtttgaa ggcccggatc
      961 gcggccggag ccaatgtcgt cacctcgatc atccctccgg aggggggatt ggcgggggtt
     1021 gcccagcatg acctggacat agggaacggc aggaggaccg tccctgcggt gaccaggatc
     1081 ttggagacga tggacatgga ggtcgcatcc atctcgcatt acaaggatta catccagaag
     1141 tggagggagg gcagggccgt gggctagctc acggggttga gggaagggac cgatggtccc
     1201 ttgcacgttg aaaggcgaag gacgccgttt acgcagatct gtagctccga gcgcaagcgt
     1261 tggtcgcacg gcgtcgcgcc tgcccccccc cacctggaag aggacgcctc gtcccattgg
     1321 accgggggga cacgccctgt tccgagcttt tgggccgagc gaacggtcgg aggcacagaa
     1381 tatgaaagca aaaccatctc acgatgaacg ccagggggcc cgagccattt ctatctctcc
     1441 ggtccaagag tcccttcgac cccctggcaa tgcatttaat cctttcatgc gaaacggagg
     1501 gttatcatga ccagactgac caaggagatg attgaggaga tcgaggggga tttggagatc
     1561 acggagcacc gtcttcgaaa ggcgataggc atggacctac ggtccctggc gctgagggcg
     1621 gtcggcctag aggccgatgc agatgtttcg aagttcagcg cagcgatcgt cccggtcacg
     1681 agcgggttgg gggtgacccc cggtttcgtc agctccgtcg cgaccatcct tgcccgcctg
     1741 ggaatggagg cggcggtcac caacgccacg gatgtggcag ggatggcgga ggggatcagg
     1801 atgggctcgg acatggtgtt catggccgat gacaatgtat tcatggccca tccggtagga
     1861 acctgggagg taatcgacaa tgccgcatgc acggccctgg gattcgttca ggccttggaa
     1921 gaggcggcgg acgggctggg cgggaaggag gtggtcgtta taggactggg ccgggtcgga
     1981 tccttcgcgg tcgctgaact cttggcaaga ggagcgaggc ccatcggcgt ggacatcgac
     2041 cccgtggcat tgatgagggc cagggagagg ttcggcatcc cttgttcgag cgatctaaag
     2101 cgggtgatgc cagccgcgga cctggtcgtc catgcatgtc ccgccccggt aaggggggag
     2161 ctggtcaagg agggggcgat catctccgcg ccaggggttc catgcgggtt cgatgccatt
     2221 ggttatggaa gagcggggct gatcatgcac gacatgctcc ccacgggcgt ggccgtgatg
     2281 gcagcgcagg cctgccgggc gctaatggcg gccaatgtgg cgaactatcc cgtggaatgg
     2341 gggctagccg ttcatgagtg agctcggcat cgccttggac atcgggacca gcggcttccg
     2401 ggcccaggcg atcgacctcg cgaccggcga ggtcctgtcc acggccatca ccgtgcgaca
     2461 tcccatacct ggctccaacg tcatggacca tgtcagcttc gccatcgagg tgggcgagga
     2521 cgaatgcaac cgtctgatgg tgagcaccat caatcagctc attcgcaggc
//
LOCUS       DAOSYN010000192         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_31194_length_2567_cov_3.023885, whole genome shotgun sequence.
ACCESSION   DAOSYN010000192 DAOSYN010000000
VERSION     DAOSYN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31194_length_2567_cov_3.023885"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1254)
                     /locus_tag="PLJ11_06825"
     CDS             complement(310..1254)
                     /locus_tag="PLJ11_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOO04414.1"
                     /translation="MLFDVLFLLGGLGLLIIGANVLVKGASALAASLGVSTFVIGLTV
                     VAMGTSAPEASLAVISSWEGDASISFGNIIGANLTNIGVVLASACLVCPIPSGMTLLR
                     REAVFLVVSIAAVASLGLNGSIDLGDSLVLIVAGLAFNYFVVRSAKLERAAKYAEEGG
                     GRVGMGRGKMAISIAGGLALLIIGSELVLQGGVGLATALGVDEFIIGLTVVAIGTTMP
                     ELAVSLSSACRLEPDLLLGNVLGSNIFNSLFIIGLAGTFSPVSVSSSFYTIIMPFTLV
                     MYLVLIAFMYKGRKLNRATGGMLATIYVAYLLGVLLMA"
     gene            1316..2563
                     /locus_tag="PLJ11_06830"
     CDS             1316..2563
                     /locus_tag="PLJ11_06830"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HOO04415.1"
                     /translation="MKFLVETYGCTMNHGESTEFSAHLRSLGHEEVLSEGEAEVVLVN
                     TCTVIASTERKILRRLRHLRDEGKGLVIVGCMASVDRPRLQEEFPGALICGTADYPRL
                     MSVLDDRFERCGPATPLNRISPLVLPIAQGCNGACTYCITRLARGDLRSYPLDTLVQR
                     AAKALEQGAKEVLVTSQDTAAYGMDGDERLPDLLKALCALPGDFRVRVGMMNPDNLRE
                     ILDEMVEAYRHPKVYRFLHLPVQSGSDPIIERMGRAYSADAFLSMVRRMRSAVPELTL
                     STDVITGFPGETDEDHRATVALMEALRPNIINVTRFSPRPGTVAAAMKGQVPGWVAKE
                     RSRELTRLRFRISSEINASKVGRTYSALVDERGKGESVMARTDDYLPVVLRGEHRLWQ
                     RVNVRITGHAPTHLYGVVQRMED"
BASE COUNT          562 a          819 c          751 g          435 t
ORIGIN      
        1 catcaactgg cagtgaccgg gctagcccgg tcactgtaat tttgcaacaa ggcccactaa
       61 tatgaccgag accacctacg gacgtatcaa gagcggtcaa acgctgatga tcctcaataa
      121 actttgggag aacgatgcag cttcatcgaa cccccaatat gacaaaaacc ctctgattga
      181 aagtaaatat gaaatgactg gctatcaata gaggcgcgtc ccccccttcg cgccatcctc
      241 gtcctcattt tcaatccttc cgaccagctc gcacccacgc tccatgccag ggcgaatttc
      301 ctgtgttgtt caggccataa gcagcacgcc aagcaaatat gcgacgtata tcgtggccag
      361 cattcctccg gtggccctgt tgagcttccg tcccttgtac atgaacgcga tcagcacaag
      421 atacatcacc aaggtgaagg gcatgatgat cgtgtagaag ctcgatgaaa cggagaccgg
      481 cgagaacgtt ccagccagac cgatgatgaa caggctgttg aagatgttgc tccccaacac
      541 gttccccaac agcaggtccg gctcgagccg gcatgcggag gaaagcgaga cggccagctc
      601 gggcatggtg gtccctatgg ccaccacggt caggccgatg atgaactcgt caacccccag
      661 ggcagtggcc aaccccaccc ctccctggag gacgagttcc gacccgatta tcaagagggc
      721 caatcccccg gcgatcgata tggccatctt ccccctcccc atgcccaccc ttcctccgcc
      781 ctcctccgca tacttagccg cccgctccag cttggcggac ctcaccacga agtagttgaa
      841 ggccaggcca gcgacgatca ggaccagcga atcgccgagg tcgatgctgc cattcaaccc
      901 gagggaggcc accgctgcga tggatacgac gaggaacacc gcctccctcc gcagaagggt
      961 catcccagat gggatcgggc agacgagaca ggccgaggcc aggacgaccc caatattcgt
     1021 tagattcgcc ccgatgatgt taccgaagga gatggaggcg tccccttccc agctggagat
     1081 gaccgccaag gaggcctccg gagccgatgt gcccatggcc accacggtca ggccgatgac
     1141 gaacgtgctt acccccaggg aggcggccag ggcggaggcg cctttgacca gcacgttcgc
     1201 gccgatgatc aggagaccga gacctcccaa caaaaaaagg acatcgaaca acatgctcca
     1261 tcgcctttag agtgttcaga gtatctataa tagaaggctt gcgtatcagc cgcccgtgaa
     1321 gttcctagtg gagacctacg gctgcacgat gaaccacggg gagtcaacag agttcagcgc
     1381 ccacctgagg tccctggggc atgaggaggt cctctcggaa ggggaggcgg aggtggtgct
     1441 cgtcaacaca tgcacggtga tcgcctcgac cgagaggaag atcctccgac gccttcgcca
     1501 tctcagggac gaggggaaag ggctcgtcat cgttgggtgc atggcctcgg tggaccgccc
     1561 ccgccttcag gaggagttcc ccggggcgct catatgcggc acggccgact atccgaggct
     1621 catgtccgtt ctggacgatc ggttcgaaag gtgcggaccg gcgacccctt tgaacaggat
     1681 ctcgcccttg gtgctcccca tcgcccaggg ctgcaacggc gcctgcacct attgcatcac
     1741 caggttggcc cggggggacc tgaggagcta tccattggac acgttggtcc aaagggcggc
     1801 caaggcgctc gagcaagggg ccaaggaggt cctcgtcacc tcccaggaca ccgcagccta
     1861 cggcatggac ggggacgagc gactgcccga cctcctcaag gccctatgcg cccttcccgg
     1921 ggacttccgg gtccgggtcg ggatgatgaa cccggacaac ctcagggaga tactggacga
     1981 aatggtagag gcgtatcgcc accccaaggt ctaccgcttc ctgcacctcc cagtgcagag
     2041 cgggtccgat ccgatcatcg agaggatggg cagggcatat tcggcggatg ccttcctatc
     2101 aatggtgcgc aggatgcgct ccgccgttcc cgagctcacg ctctccacgg acgtcatcac
     2161 cgggttcccg ggcgagaccg atgaggacca cagggcgacg gtcgccctca tggaggccct
     2221 gcggcctaac atcatcaacg tcacgaggtt ctcccctcgc ccggggacgg tggccgccgc
     2281 catgaaagga caggtccccg gctgggtggc caaggagagg tcccgggagc tcacccgcct
     2341 ccgcttccgc atctcctccg agatcaacgc ctccaaggtc ggccggacct attcggcctt
     2401 ggtggacgag cggggaaagg gcgagagcgt catggcccgc accgatgact atctcccggt
     2461 cgtactgagg ggagagcaca ggctatggca aagggtgaac gtccgcataa cgggccacgc
     2521 ccccacccat ctgtatgggg tcgtccaacg gatggaagat taaatac
//
LOCUS       DAOSYN010000193         2549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_31474_length_2549_cov_4.292702, whole genome shotgun sequence.
ACCESSION   DAOSYN010000193 DAOSYN010000000
VERSION     DAOSYN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31474_length_2549_cov_4.292702"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1253
                     /locus_tag="PLJ11_06835"
     CDS             192..1253
                     /locus_tag="PLJ11_06835"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOO04416.1"
                     /translation="MEDFSHNLNPYGPPAALPQLVEEAVEHLGHYPDDSSTALREAIA
                     AHHGVAPENVIAGAGSTELIRLFPEVFLSRRARAIIPRPTFSEYAHACRMQGANIMDV
                     PLIEGEGFRLDMNAIKERLPLATALYLCNPNNPTGTVEPRRRIMELVDECEAFGKLVF
                     LDETLLELVEGHEALSCMGEVRNHSNLFIISSLTKSFGIPGLRVGYGVGSAEIVEMLD
                     RARPSWNLGAIEQHVGARLVREHQGHVQRAAEMMAAERGRIKRAVDRTGLLSPLPDQC
                     FFFFARVSDPKLSAADIVERLLPRKVLVRDCSTFGRPFDKFVRFSIKAPDRNDLLISA
                     IEGTAQEMRSTAFGARMHV"
     gene            1246..2253
                     /gene="cbiB"
                     /locus_tag="PLJ11_06840"
     CDS             1246..2253
                     /gene="cbiB"
                     /locus_tag="PLJ11_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOO04417.1"
                     /translation="MYELVPYALLTLAVALLIDRFLGEPPNRWHPVVWMGRAIAFLDR
                     RAPRGRAAREKAYGTLMALAVILLFGLATLFLTAAVRHTLGPLAWALVGGVVLKTTFA
                     IGAMAKHTEPVRAALLAGDLELGRQKAAMMVSRDVSQLDEGHVLSCAAESAAENTVDS
                     ILSPLFYHGLLGVPAAVAYRASNTLDAMVGYMDERHRHVGWFSARLDDCTNWLMARVA
                     VPFILLALALLGKDWRAGWAAARKHHDRTLSPNKGWHMAAFAGGLGIRFEKIGWYVLG
                     DGPLPSDPEVLRDTIKVMTLTAYLFVLVVVVPLSLLVGVHLQVLMEDMLWGLIAGCIG
                     G"
     gene            2255..>2549
                     /locus_tag="PLJ11_06845"
     CDS             2255..>2549
                     /locus_tag="PLJ11_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HOO04418.1"
                     /translation="MRLSEIRIDRGTVDGYRYVAIRLPRRMRTFASTILNGGLGETDS
                     VLMVQVPLHYDHAAPVEHLRTMLRELTLPEDTACFMTAAELDKAGIRLLVEPIN"
BASE COUNT          423 a          822 c          883 g          421 t
ORIGIN      
        1 cgaggagatc atccgcgccg tgtacggcgt ggaggtgatg ctcttcaagg agaagggcga
       61 gatcttcgtc atcccattaa ggccagtgga cacggggaag ggcgccgatg cagatcaaag
      121 accttgtgag aaaggaattg ttgccgatta agcggccggt ccacggggga cagggctgga
      181 gggtcccggg ggtggaggac ttcagtcaca acctgaaccc gtacggcccg ccagccgcgc
      241 tgccgcaact ggtcgaggag gccgtcgagc acctgggcca ctaccccgac gacagctcga
      301 cggccctccg ggaggcgatc gccgcccacc acggcgtggc gccggagaac gtcatcgctg
      361 gcgccggctc caccgagctc atccgcctct tcccggaggt gttcctctcc agaagggcga
      421 gggcgatcat ccccaggccg acgttctccg agtacgccca cgcgtgccgc atgcaggggg
      481 cgaacatcat ggacgtgccc ctgatcgagg gggagggctt ccgcctggac atgaacgcca
      541 tcaaggaaag gctccccctc gccacagcgc tctacctctg caaccccaac aaccccaccg
      601 gcacggtgga gcccaggcgc cggatcatgg agctggtgga cgagtgcgaa gcgttcggca
      661 agctggtctt cctggacgag accctcctgg agctcgtgga ggggcacgag gccctgtcct
      721 gcatgggcga ggtccggaac cattccaacc tgttcatcat ctcctcgctg acgaaatcgt
      781 tcggcatccc cgggctacgg gtcggctacg gcgtgggcag cgcggagatc gtggagatgc
      841 tcgaccgggc ccgcccctcc tggaacctgg gggcgatcga gcagcatgtg ggcgctaggc
      901 tggtccggga gcatcagggc cacgtgcaac gggcggcgga gatgatggcc gccgagcgcg
      961 ggaggataaa gagggcggtc gaccgcaccg ggctgctctc ccccctcccg gaccaatgct
     1021 tcttcttctt cgcccgggtg agcgatccca agctctccgc ggcggacatc gtcgagcgcc
     1081 tcctcccccg caaggtgctg gtccgggact gctccacgtt cggccgcccc ttcgacaagt
     1141 tcgtgcgctt cagcatcaag gccccggata ggaacgacct gctcatctcc gccatcgagg
     1201 gaaccgccca ggagatgcgc agcactgctt tcggggcgag gatgcatgta tgagctggtc
     1261 ccgtacgcac tgcttaccct ggccgtcgcc ctgctgatcg accggttcct cggggagcct
     1321 ccgaaccgct ggcacccggt ggtgtggatg gggagggcga tcgccttcct ggaccgaagg
     1381 gcgccgcggg ggagggccgc gcgggagaaa gcgtacggaa cgctcatggc gctcgccgtc
     1441 atcctcctct tcggccttgc aacgctcttc ctcacggccg cggtgcggca cacgctcgga
     1501 ccgctggcct gggcccttgt cggcggagtg gtcctgaaga cgacgttcgc catcggggcc
     1561 atggccaagc acaccgagcc ggtgcgcgca gcgctgctgg cgggggacct ggaactgggg
     1621 aggcagaagg cggcgatgat ggtctcgagg gacgtgagcc aattggatga ggggcacgtg
     1681 ctctcctgcg cggcggagag cgcggcggag aacaccgtgg acagcatcct ctcccccctc
     1741 ttctaccacg ggctgctcgg ggtgcccgcg gccgtggcct accgggcctc caacaccctg
     1801 gacgccatgg tcggctatat ggacgaaagg caccgccacg tgggctggtt ctccgccagg
     1861 ctggatgact gcacgaactg gctgatggcc agggtggcgg tgcccttcat cctgttggcg
     1921 ctggcgctcc tgggcaagga ctggcgggcc ggatgggcgg ccgcgcggaa gcaccacgac
     1981 cggacgctct ccccgaacaa gggctggcac atggcggcct tcgccggcgg gctcgggatc
     2041 cggttcgaga agatagggtg gtacgtcctg ggcgacgggc ccctgccctc ggacccggag
     2101 gtgctcaggg acaccataaa ggtcatgacc ctgacggcgt atctcttcgt gctcgtcgtg
     2161 gtggtgccgc tctccctgct ggtcggcgtg cacctgcagg ttcttatgga ggatatgctc
     2221 tgggggctca tagctggttg cataggtggt tgacatgagg ctgagcgaga tcaggatcga
     2281 cagggggacg gtggacgggt acaggtacgt ggcgatcagg cttccccgcc gcatgaggac
     2341 ctttgcctcc accattctca acggagggct cggggagacg gactccgtgc tcatggtcca
     2401 ggtccccctg cactatgacc atgcggcccc ggtggagcat ctgcgcacta tgttgcgtga
     2461 gctgacgctc cccgaggaca cggcatgctt catgaccgcc gccgagctgg acaaggctgg
     2521 catccgcctg ctggtggagc cgatcaaca
//
LOCUS       DAOSYN010000194         2548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_31493_length_2548_cov_2.993983, whole genome shotgun sequence.
ACCESSION   DAOSYN010000194 DAOSYN010000000
VERSION     DAOSYN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31493_length_2548_cov_2.993983"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..1697
                     /locus_tag="PLJ11_06850"
     CDS             381..1697
                     /locus_tag="PLJ11_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOO04419.1"
                     /translation="MTGTSEIVYEEVPLRLSPAEAYLRLRAAGEPSFLLESAEGASRT
                     VAYSFLGAAPVSVLRAEDEREGLDKVRRRMALRARQHSPFPFMGGLVGYFSYHLVNGI
                     EEGLSVRKGGFPAYELGEYDSGLIYDHSAFKVYHFHPRDGAGRLPLLEQEGEVEDFQA
                     GELLSATSRESYEAGVEDLHRRISEGEVLQTVLSGHEERRFSGDPFRMYLRLRAINPS
                     PYMFYLDFGERKVLGSSPETLVTVRDRGAVTFPIAGTRPLGTDLRDRMRNRSSMLTDQ
                     KERAEHMMLVDLARNDLGKVCEFGSVEVADLMRVEEYSHVQHMVSRVRGILREDRDAL
                     DALAAVFPAGTVSGAPKHRAMRLIEMLEERGRGPYAGAVGYVSDSGDLDTAISIRSAF
                     VNGDRMVLQAGAGIVLDSVPEREYQECQGKLAALHSALGLAEGAGP"
     gene            1694..2284
                     /locus_tag="PLJ11_06855"
     CDS             1694..2284
                     /locus_tag="PLJ11_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOO04420.1"
                     /translation="MRPRVLVVDNYDSFVHNLAHCLARAGAEPVVVRNDHLDLGSLRD
                     RFQGIVVSPGPGHPANGTDFGACLELLRTVSPSVPTLGVCLGHQGIAQAFGGRVGRAP
                     EPLHGKATPIHHDGRGVFKGLPSPMLVGRYHSLCVLDVLPACMVVTARAADGTVMGLR
                     HLAYPIEGVQFHPESVLTEHGQGIVDNFVASLGVGG"
     gene            2281..>2548
                     /locus_tag="PLJ11_06860"
     CDS             2281..>2548
                     /locus_tag="PLJ11_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04421.1"
                     /translation="MSEAERTVQARAFAYEIIEGRMSDRAIADRLLSMRARGESPEDL
                     LGMLSVFYPQSQRVRTRHPVVIDLCGTGGAAVRTFNISTISSFVV"
BASE COUNT          415 a          786 c          883 g          464 t
ORIGIN      
        1 gccattggcc cgggggcttc cattgaacgc caccactttg attgccatcc ctccctccaa
       61 cgctccggac cgtcttatcg tttgtccttt tccagcccat accttttttt gggatgccga
      121 cagcccctcg gggctccccc ttgaacgggc gacggtccgg gaaggcggcc gggcctgccc
      181 tcctccgttc gcctgtcgcc acggaaggac cgtcctcgtt ccaaccggcg ccagggcagg
      241 cctgaactgt cctggagcct ccgctctgta cgggtcgggg tcgcccgcag tttcggggtg
      301 gtcgaggcta ttatagattc atttatgtat tataataatc aataatgctt atatatgttc
      361 actaatgtgc atgaaataca atgaccggaa catcggagat cgtatatgag gaggttcccc
      421 tccggcttag tcccgcggag gcctaccttc gactgcgggc ggcgggcgaa ccctcgttct
      481 tgctggagag cgcagaagga gcctcgagga cagtggccta ctcgttccta ggggccgcgc
      541 ccgtatccgt cctcagggcg gaggacgagc gggaaggcct ggataaggtc cggcggcgga
      601 tggccctccg ggcccgccag cactccccgt tcccgttcat gggtgggctc gtgggctatt
      661 tctcatatca tctggtcaac gggatcgagg aagggttgag tgtgcggaag ggcgggtttc
      721 ccgcctatga gctgggggaa tacgatagcg gcctgatcta cgaccattcg gccttcaagg
      781 tctaccattt ccatccccgc gatggggcgg ggaggctgcc ccttctggag caggaagggg
      841 aggtagagga ctttcaagcg ggcgaactgc tatcggccac gtccagggag agctacgaag
      901 cgggggtcga ggacctgcat cgacggatat cggaggggga ggtgctgcag acggtccttt
      961 cagggcatga ggagcgccgc ttctccggcg accccttccg gatgtacctg cgcctgcggg
     1021 ccataaatcc ctcgccctac atgttctatc tggacttcgg ggagcgcaag gtgctcggct
     1081 caagccccga gacgctggtc accgtgcgcg accggggagc ggtgactttt ccaatcgcgg
     1141 gcacgcgccc cctcggcacg gaccttaggg acaggatgcg caaccgcagt tcgatgctga
     1201 ccgatcagaa ggagagggcc gagcacatga tgctggtgga cctcgcccgc aacgacctgg
     1261 ggaaggtgtg cgagtttggg agcgtcgagg tggccgacct gatgagggtg gaggagtaca
     1321 gccacgtgca gcacatggtc tccagggtgc gcggcatcct tcgggaggac cgcgatgcgc
     1381 tggacgcgct ggcggcggtg ttcccggcgg gaacggtgag cggggcgccc aaacatcggg
     1441 cgatgcgcct catcgagatg ctggaggaga gagggagggg gccgtacgcg ggcgcggtcg
     1501 gctatgtttc ggacagtggg gaccttgaca ccgccatctc catccgctcc gccttcgtca
     1561 acggcgaccg gatggtgctg caggccggcg ctgggatagt gctcgattcc gtcccggaga
     1621 gggaatatca ggaatgccag gggaagctgg ccgccctgca cagcgcccta ggcctggccg
     1681 agggggcggg gccatgaggc cgagggtgct ggtcgtggac aattacgatt cgttcgtgca
     1741 caacctcgcc cattgccttg cccgggcagg cgcggagcca gtggtggtcc gcaacgacca
     1801 tctggacctg ggatcgctcc gggaccgctt tcaggggatc gtggtctccc cggggccggg
     1861 ccatcccgcg aacggaacgg acttcggcgc atgcctcgaa ctgctgagga ccgtttcccc
     1921 ctccgtcccc acactggggg tgtgcctggg gcaccagggc atagcgcagg ccttcggcgg
     1981 cagggtgggg cgggcgcccg agcccctgca cgggaaggcc accccgatcc accatgacgg
     2041 gaggggggtg ttcaagggat tgccgtcccc catgctcgtc gggaggtacc attccctgtg
     2101 cgtcctcgac gtccttcccg cctgcatggt cgtcaccgcc cgcgccgcgg acggtacggt
     2161 catggggctc cgccatctcg cctaccccat cgagggcgtg cagttccacc cggaatcggt
     2221 gctcaccgag cacgggcagg ggatcgtgga caacttcgtg gcctccctgg gggtgggggg
     2281 gtgagcgagg cggagcggac cgtgcaggcc cgcgcgttcg cgtacgagat catcgagggg
     2341 cggatgtcgg accgggccat cgccgatcgc ctgctctcca tgcgggcccg gggcgagagc
     2401 cccgaggacc tcctgggcat gctctccgta ttctatcccc aaagccagcg ggtccggacc
     2461 cgtcatccgg tggtcattga cctctgcggg accggagggg cggcggtgcg gaccttcaac
     2521 ataagcacca tcagctcctt cgtggtgg
//
LOCUS       DAOSYN010000195         2542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_31596_length_2542_cov_3.630076, whole genome shotgun sequence.
ACCESSION   DAOSYN010000195 DAOSYN010000000
VERSION     DAOSYN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31596_length_2542_cov_3.630076"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /gene="glmS"
                     /locus_tag="PLJ11_06865"
     CDS             <1..1423
                     /gene="glmS"
                     /locus_tag="PLJ11_06865"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOO04422.1"
                     /translation="VVVHLIEGHFKGDLRHAMADTLKEIKGTYAIAAVLEGSEEIVAA
                     RKENPLVIGLGVQENFLASDVTALLSYTNKVLYVMDGETVSLTPNGVAIFDSEGAQVK
                     REPSLVSWSAEDAQKGGFEHFMLKEIFEGPNAIHNTLLGALDLIENGEVLPRTDFQSV
                     KLVACGSSYHAAMVGKYAMEEFAGVPTTLELASEYRYSPGARETPLVILITQSGETAD
                     TLAAAREARRRGCHTLAVTNVVGSTITREVDEVLYTRAGPEIGVAATKTFLTQLVAMY
                     LVAIRIGYGNGTLSHASMRSALSSLRQSSNMVQSVLNRTHSIEEASRMLDRAGHCFFI
                     GRNINYPTMLEGALKLKEISYIHAEGFAAGELKHGPLALLDATTPVIAVAIKGDHTYE
                     KMLANISEVAARDSPVLGIGVEGDTDLGRLCERMIAIQPVEPVFSPLPVTVALQALAY
                     YVARRRGCSIDKPKNLAKSVTVE"
     gene            complement(1424..2413)
                     /locus_tag="PLJ11_06870"
     CDS             complement(1424..2413)
                     /locus_tag="PLJ11_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOO04423.1"
                     /translation="MDRDEVISASDLERYGYCPLSWWLGRTFQSHTPEQERGELVHRN
                     KAEEYRRIRQMEQRAWDWERMVFLFSLVATVLAVTGISLMRATGTTDGDWLLPLLSII
                     WFFAVIAMLYLSAQRGRAIIEVRRWQTVAILGLVLMVVAMNIGNLFEADPAVSMIMEV
                     LALVWLIAASVALYISLSTSRQVEDRKDEEGLEGEVRYVGTDDSPLYYSERYGLSGRP
                     DFVLDINGQLIPGDLKTGRTPKGPLFSHILQVAAYCLLIAEDSGRRPPYGLLRYGEEE
                     HEIEFDPELERILLEKLEEMRALMRTGDVHRNHNRPGKCAGCSRRDLCPERLV"
BASE COUNT          502 a          813 c          784 g          443 t
ORIGIN      
        1 ggtggtagtg caccttatcg aaggccattt caagggcgat ctccgccatg ccatggccga
       61 caccctcaag gagataaagg gtacctatgc catcgcggcg gtgctcgaag gcagcgagga
      121 gatcgtcgcc gcccgcaagg agaaccccct tgtgatcggc ctcggcgtcc aggagaactt
      181 cctggcttcg gacgtcaccg ccctccttag ctataccaac aaggtccttt acgtcatgga
      241 tggggagacc gtgtccctta ctcccaatgg agtggccata ttcgacagcg agggggccca
      301 ggtcaaacgc gaaccctcct tggtgtcctg gtccgccgag gatgcccaga agggagggtt
      361 cgagcacttc atgctcaagg agatattcga ggggccgaat gccatccaca acactctgct
      421 gggggcattg gacctcatcg agaacgggga ggtcctcccg cgcacagact tccagtccgt
      481 gaagctggtg gcctgtggct cctcctatca tgcggccatg gtgggcaagt acgccatgga
      541 ggagttcgcc ggcgtgccga ccaccctgga gctggcctcg gagtaccgat actcgcccgg
      601 cgcccgggag acgccattgg tcatcctcat cacccagagc ggggagaccg ccgacacgct
      661 cgccgccgcc agggaggcca ggcgcagggg gtgtcacact ctggcggtga caaacgtggt
      721 cggctcgacg atcaccaggg aggtggacga ggtgctgtac acccgcgctg ggccggagat
      781 aggggtggcg gccacgaaga ccttcctcac ccaattagtg gccatgtacc tggtggccat
      841 acgcatcggc tatgggaacg gaacgctgag ccatgcctcc atgcgcagcg cgctatcctc
      901 cctcaggcaa tcctccaaca tggtgcagtc agtgctcaac cgcactcaca gcatagagga
      961 ggcgagccgc atgctggaca gggcaggtca ttgcttcttc atcgggcgta acatcaatta
     1021 cccgacgatg ctggaaggcg ccttgaagct caaggagatc tcctacatcc atgccgaagg
     1081 gttcgccgcc ggggagctga agcatggccc gctcgccctg ttggatgcca ccacccctgt
     1141 catcgccgtc gccataaagg gggaccatac ctacgagaag atgctcgcca acatctccga
     1201 ggtggcggcc agggacagcc ctgtactagg gataggggta gagggggaca ccgacctggg
     1261 aaggctctgc gaacggatga tagccattca gccggtcgag ccggtattct cgcccctccc
     1321 cgtcaccgtg gccctgcaag ccctcgccta ttacgtggca aggcgccgcg gatgctccat
     1381 cgacaagcct aagaacctgg ccaagagcgt caccgtggaa tgatcatacc agccgctccg
     1441 ggcaaaggtc acgacgggaa cagccggcgc acttcccggg gcggttgtga ttgcgatgca
     1501 catccccggt tcgcatgagg gcacgcatct cctccagctt ctccaggagg atccgctcga
     1561 gttcggggtc gaactctatc tcatgctcct cctccccgta gcgcaggagt ccatagggag
     1621 ggcgcctacc ggaatcctcg gcgatgagga ggcagtaggc cgccacctgg aggatgtgcg
     1681 agaacagcgg ccccttcggg gtcctccccg tcttcaggtc ccccgggatg agctgtccgt
     1741 tgatgtccag gacgaagtcg gggcggccgc tcagtccgta cctctcagag taatagagcg
     1801 gggagtcatc ggtcccgacg taccggacct ccccctccag tccctcctcg tccttccggt
     1861 cctcgacctg cctggaggtc gagagcgaga tgtagagggc aacagaggcg gcgatgagcc
     1921 ataccagggc caggacctcc attatcatcg atacggccgg gtcggcctcg aaaaggttcc
     1981 cgatgttcat ggccacgacc atgagcacga gcccaaggat ggccacggtc tgccagcgac
     2041 ggacctcgat tatcgcgcgc cccctctgcg ccgacaggta cagcatggcg atcaccgcga
     2101 agaaccagat tatgcttagg agcggcagca gccaatcccc gtcggtggtc ccggtggccc
     2161 tcatgaggga gatgccggtg accgccagga cagtggccac cagggagaag aggaagacca
     2221 tcctctccca gtcccaggcc ctttgctcca tctgacggat gcggcggtac tcctccgcct
     2281 tgttcctgtg gacaagctcc ccccgctcct gctcaggcgt gtgggactgg aacgtcctgc
     2341 ccagccacca gctgagcgga caatagccat accgctccag atcgctggcg gagatgacct
     2401 cgtcccggtc catgggctaa aatatggatg gctggtatat aatgggtgtt gggccgtcag
     2461 tccgcgcacc ggcagctcag cccggtcagc gaggagtccg agatgaagac cccgtcctcc
     2521 atggttatct cgtcgacgaa gc
//
LOCUS       DAOSYN010000196         2542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_31599_length_2542_cov_3.129071, whole genome shotgun sequence.
ACCESSION   DAOSYN010000196 DAOSYN010000000
VERSION     DAOSYN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31599_length_2542_cov_3.129071"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..>2542
                     /locus_tag="PLJ11_06875"
     CDS             314..>2542
                     /locus_tag="PLJ11_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04424.1"
                     /translation="MQGTSRTFGLAKKAVMYIGILALALVLLLLLGAGQAGAEEVTGD
                     ITTDTTWVSGDVYNISSSVTVVDGATLEIQAGATVIFNGTNYLNVEGAMFVNGTAGQP
                     VIFTNNASKGMGGGIVFLGGSDGSVSFAHMSNCTMAIYFDNVTIPCSDIVIDSSNCGL
                     YYSFYGMEQNITLALSDITMTDTDYAIIILQYNGTVDLAVTDSMLKGGMYGLYVETWN
                     GGSAVITNTQFLGQEYLAYHFYSLYGEMDVNIADSVFDGSEAADMTVYTVEEVENTFE
                     VIGWNNWNWGVDAWSPSTTWVDEALIDLPFAFAYNGFKYSSVTISSDGYLNFGSDNCI
                     EPVGNTDLIYSGYSYARQCYGYKVAEDNRSVMFHWYASDWYSGPYLSIAFQVVLFADG
                     TIQFNYGDMEAYEYYYADHSYGLRTDGSTIYDLRSLYGMPKYEADGMAFLFTPLPLSN
                     GVAAQLWAEEGAINVEITDSTFTSYYGGGISVRANNGGLALTVTGSEFSKIYNNEFNA
                     LDLYNFNGAAELTMTDNTFRLIWGLAISLDLTSTEGWEGTIDVSDSVFDKVAYVLYAE
                     INVNDNSGRTGDDSLNATVIFRNNMLTDSFGMFCEIGLELDDPVNWTVTVAQTMTGNT
                     MVQENYMGSWSFPDNYPEAPGLGVFVWIEQEGEDENSVTLEQTATVTGNHIECPWSMG
                     IVGIAVGNEIVNLYGDTTRTFAVDISRNFVNCTDLAGIAAVSMMTAGTGFVVDDAIVT
                     MND"
BASE COUNT          584 a          695 c          717 g          546 t
ORIGIN      
        1 gcctgttctc ggccaggacg tccgtctcca tgctgatgtg ggtgatcttg tgcacaaggg
       61 cggattcccg ctcttccact aagaaccttt cccccgaacg ttcgagccgt ccgccgcgcg
      121 tgcccatcgg gaacggcaca ctgacatggg aacaaaggta cagcaatacg ccttcgttcc
      181 gtttggttcg tttggttcaa ttaatcaaaa gaatctataa atattgttat gtaaatatga
      241 gagtcaacca atccaaggtt ccgaaaggtg gatgggacca aaatggggga ctcttatgaa
      301 cgattcgggt ttattgcaag gtaccagtag gactttcggc ctggccaaga aggccgttat
      361 gtacatcggc atattggcgc tcgccttggt cctgctcctt ctcctagggg caggccaggc
      421 gggcgctgag gaggtcacag gggacatcac gacggacacc acatgggtct ctggcgacgt
      481 gtataatatc tcttcgagcg tgacagtggt cgatggggcc acgttggaga tacaggccgg
      541 agcgaccgtt atcttcaacg gcaccaatta tctcaatgtc gaaggagcta tgttcgtgaa
      601 cgggaccgcg ggccaaccgg tcatcttcac gaacaatgcg tcaaagggga tgggcggcgg
      661 catagtgttc ctcggagggt ccgatggctc tgtatccttt gcgcatatgt ccaattgcac
      721 catggcgata tactttgaca atgttaccat tccctgctcc gacatcgtga ttgactcttc
      781 caattgcgga ctgtactata gtttctacgg aatggaacag aacatcacat tggccctttc
      841 ggacataacg atgaccgata ctgactacgc gatcatcatc cttcagtata atggaaccgt
      901 ggacctggct gtgacggatt ccatgctgaa gggcggtatg tatgggctgt atgtcgaaac
      961 ctggaatggt ggctcggccg taataaccaa cacccaattc ctcgggcagg aatatctggc
     1021 ctaccatttc tactccttgt acggagagat ggacgtgaat atagccgaca gcgtgttcga
     1081 cggcagcgag gccgcggaca tgaccgtgta cacggtcgag gaggtggaga acacctttga
     1141 ggtgatcggc tggaacaact ggaactgggg ggtggacgcc tggagccctt ccaccacgtg
     1201 ggtggatgaa gcactgatag acctcccctt cgccttcgcc tataacgggt tcaaatattc
     1261 ttccgtcacc ataagctccg atggctatct taactttggc agcgacaact gcatcgaacc
     1321 tgtggggaac accgacctaa tctattccgg ctactcctat gccaggcagt gctacggcta
     1381 caaggttgcc gaggacaaca gatcggtgat gttccactgg tacgcgtccg attggtattc
     1441 cggcccctat ctaagcatag ccttccaggt cgtgctgttc gcggacggca ccatccagtt
     1501 caactacggg gacatggagg cctatgagta ctattatgcc gatcactcat atggactaag
     1561 gacggacggc agcaccatct acgacctccg aagcctgtat ggcatgccca agtacgaagc
     1621 cgacgggatg gccttcctct tcaccccgct gcccctctcc aatggggtgg ctgcgcagct
     1681 atgggcggaa gagggagcaa tcaacgtgga gattaccgac agcaccttca ccagctacta
     1741 tggtggtggg atctcggtac gggccaacaa cgggggcctc gccctgacgg tcaccgggag
     1801 cgagttcagc aagatctata acaatgagtt caacgccctc gacctctaca acttcaacgg
     1861 ggcggccgaa ctgaccatga ccgacaacac cttcaggctc atctggggcc tggccatctc
     1921 cctcgacctg acctcgacgg agggctggga agggaccata gacgtgtccg attccgtgtt
     1981 cgataaggtc gcctacgtcc tctacgcaga gatcaatgtc aatgacaaca gcggccggac
     2041 cggcgacgac tctttgaacg caacggtcat cttccggaac aacatgctga ccgactcctt
     2101 cggcatgttc tgcgaaatcg ggctggagct cgacgatccg gtcaactgga cggtcacagt
     2161 ggcccagacg atgacgggca acacgatggt ccaggagaac tatatggggt cctggtcgtt
     2221 cccggacaac tatccggaag cccccggcct gggagtcttc gtttggatcg aacaggaggg
     2281 cgaggacgag aacagcgtga ccctggagca gaccgccacc gtgaccggca accacatcga
     2341 gtgcccctgg agcatgggca tagtgggcat agcggtgggc aacgaaatcg taaatcttta
     2401 tggggacacc acaaggacct ttgcggtcga catctctaga aacttcgtaa attgcactga
     2461 tctggcgggc atcgctgcgg tatccatgat gaccgcgggc accgggttcg tcgtggacga
     2521 cgcgatcgtc accatgaatg ac
//
LOCUS       DAOSYN010000197         2525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_31846_length_2525_cov_3.894737, whole genome shotgun sequence.
ACCESSION   DAOSYN010000197 DAOSYN010000000
VERSION     DAOSYN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31846_length_2525_cov_3.894737"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1316
                     /locus_tag="PLJ11_06880"
     CDS             54..1316
                     /locus_tag="PLJ11_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOO04425.1"
                     /translation="MGGMLGTVVISTFYVVPQIVGAGALLQLLLGWNYTFSVLVIGVL
                     VIAYVTTGGMRATTYNQIIQGLVLWSAMLIIMVLMLGVSFGFDPGEVLSHADDMIPPK
                     LASDLLVNDPSVPDLSTLTPDEAISFVSSRLTGEATALTPGTFAPNWMNTMALAAGLV
                     FGTAGLPHVLTRYYTVKRPKDARTSTIGVLLMIGSFYIMTVFVGLGAMYVLYPDLMGY
                     FLGGNSSIAQNMAVPLLAERTGGEIMMGIAIGGAFCAILSTVAGLLITVGTTVSHDFY
                     KEVINKSASDRREVLVAKLSIVAMGALAVVISLGMADQNVSYLVTLAFGMAASVIFPV
                     LFMSIWWKRYTRQGALVTMTVGLIVSVVFVAATLMGVESVLGLPVLVNPALYSLPAAI
                     IAGVAVSYLTKDVGDVENFMRKAHSRTE"
     gene            complement(1439..>2525)
                     /locus_tag="PLJ11_06885"
     CDS             complement(1439..>2525)
                     /locus_tag="PLJ11_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOO04426.1"
                     /translation="TERLAEIAKGLPRVARVSTVDFACSPAARAAIANDVKEHGLDRV
                     LVAACSPRLYLREFQQTLEEADRKGCMVEMCNIREQCAWVHFDDREAATAKAEDMLRM
                     SHARLQDQTWTEKANVSRVNKLRCTGCGICESVCNFNAIHVSPDKEFNGLRKANVNIN
                     ACEGCGACVAACPTAALDQACFSNQQMVSQIETFLRDGRMAFPKVLVFSCNWCSYAAA
                     DTAGLKRMAMDPHFCALRTMCSARVDPEWVLKALSKGADGVLVLAGHPGRCHYEIGNL
                     RTRKRMALLHNYLDQMGFDPDRFRIAYVDSEEAEAYVRTVNEYVDKVRALGPNPVDPR
                     TVVSPKRLEMPWLDIKPPKRWDEGLEL"
BASE COUNT          417 a          803 c          817 g          488 t
ORIGIN      
        1 ccaggaccga gaaggtgtag ttccagccca ggagcagctg caggtccctg cgtatgggtg
       61 gaatgctggg gacggtggtg atctcgacct tctacgtcgt cccgcagatc gtgggggcgg
      121 gcgcactcct gcagctgctc ctgggctgga actacacctt ctcggtcctg gtcatcggag
      181 tgctcgtgat agcgtacgtc accacgggag ggatgcgcgc caccacctac aaccagatca
      241 tccaagggct ggtgctctgg agcgccatgc tcatcataat ggtcctgatg ctgggcgttt
      301 ccttcgggtt cgacccgggc gaggtgctct cgcatgcgga cgatatgatc ccgcccaagc
      361 tggcctccga tctgctggtc aatgatccca gcgtcccgga cctctccacc ctgacgccgg
      421 acgaggcgat ctccttcgtc agctcgaggc tcacggggga ggccaccgcg ctcacgcccg
      481 gaacgttcgc gcccaattgg atgaacacca tggccctggc cgccgggctc gtcttcggaa
      541 cggccgggct gccgcacgtg ctcacccggt attacacggt gaagaggccc aaggacgccc
      601 gcacctccac catcggggtg ctgctcatga tcgggtcctt ctacatcatg accgtgttcg
      661 tcgggctcgg ggcgatgtac gtcctttacc cagacctgat ggggtacttc cttggaggga
      721 attcgtccat cgcccagaac atggccgtgc ctcttctggc cgagcgtacc gggggcgaga
      781 taatgatggg gatcgccatc gggggggcgt tctgcgccat cctcagcacg gtggccgggc
      841 tgctcatcac cgtcgggacg acggtgagcc acgacttcta caaggaggtc atcaacaaga
      901 gcgcgtccga ccggcgcgag gtgctagtgg cgaagctgtc catcgtggcg atgggggcgc
      961 tggccgtggt catcagcctg ggcatggccg accagaacgt gtcctacctc gtcaccctgg
     1021 cgttcggcat ggcggccagc gtgatcttcc cggtgctctt catgagcatc tggtggaaga
     1081 ggtacacccg gcagggcgcc ctggtgacca tgacggtcgg cctgatcgta tccgtggtgt
     1141 tcgtggcggc caccctgatg ggggtggaga gcgtgctggg gctcccggtc ctggtcaacc
     1201 ccgcgctgta cagccttccc gcggccataa tcgccggggt ggcggtgagc tacctgacca
     1261 aggacgtggg ggacgtggag aacttcatgc gcaaggccca cagcaggacc gaatgaaacc
     1321 tcttatccca acttttttcc ttgtttttat ttcatatatt tatatttatc tcactatgaa
     1381 tgatcgcggg cgaggatggt caaagaagga caggggaaca ggagggcggg gaccggggct
     1441 agagctccag tccctcgtcc catcgtttgg gcggcttgat gtccagccag ggcatctcca
     1501 gccgtttcgg ggacaccacg gtgcgcgggt cgaccgggtt cggccccagc gcccggacct
     1561 tatcgacgta ctcgttgacc gtgcggacgt acgcctcggc ctcctctgag tccacgtagg
     1621 cgatgcggaa gcggtcggga tcgaagccca tctggtccag gtagttgtgc agcagcgcca
     1681 tcctcttccg cgtgcgcagg ttgccgatct cgtaatggca ccggccgggg tggccggcga
     1741 ggaccagcac gccgtccgcg cccttggaca gcgccttgag gacccattcc gggtccacac
     1801 gggcggagca catggtgcgc agcgcgcaga agtgggggtc cattgccatc cgcttgagcc
     1861 cggccgtatc cgctgccgcg taggagcacc aattgcacga gaagacgagg accttgggga
     1921 aggccatcct cccgtccctc aggaacgtct cgatctggga gaccatctgc tggttcgaga
     1981 aacatgcctg gtccagggcg gccgtagggc acgccgccac gcacgccccg cagccctcgc
     2041 aggcgttgat gttgacattg gccttcctca ggccgttgaa ctccttgtcc ggggagacgt
     2101 ggatggcgtt gaagttgcac accgattcgc agatcccgca gccggtgcag cggagcttgt
     2161 tgaccctgga gacgttggcc ttctccgtcc atgtttggtc ctgcaggcgg gcgtgggaca
     2221 tccgcagcat gtcctcggcc ttggcggtgg ccgcctcccg gtcatcgaag tggacccagg
     2281 cgcattgctc ccggatgttg cacatctcca ccatgcatcc tttgcggtcg gcctcctcga
     2341 gcgtctgctg gaactccctc aggtagagcc tcggggagca ggcggcgacc agcacccggt
     2401 ccaacccgtg ctccttgaca tcattggcga tcgccgcgcg cgccgccggc gagcaggcga
     2461 aatccaccgt ggagacgcgc gccaccctgg gtagcccctt cgcaatctcg gccaaccttt
     2521 cagtg
//
LOCUS       DAOSYN010000198         2495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_32319_length_2495_cov_3.684426, whole genome shotgun sequence.
ACCESSION   DAOSYN010000198 DAOSYN010000000
VERSION     DAOSYN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32319_length_2495_cov_3.684426"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLJ11_06890"
     CDS             complement(<1..440)
                     /locus_tag="PLJ11_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04427.1"
                     /translation="MFHPDRDLKQTIISMLSEEGRSISYLSKELKKRGFDMHRLILTG
                     YLRALSDLNILKEKEVPPAKVYTAVKGKEKDIYIRIAEHARSISPERSGELTLYALYR
                     LFRRPIFKEELERAGVLGLSPGVQAKEEEIADARKFMLKAGFKVP"
     gene            654..>2495
                     /gene="nrdD"
                     /locus_tag="PLJ11_06895"
     CDS             654..>2495
                     /gene="nrdD"
                     /locus_tag="PLJ11_06895"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOO04428.1"
                     /translation="MKSAEATRNANMDRESTEISLFVRTSDEEIKKWDKSRIFDALMR
                     ETDISEDAAAIVAREVEKMIGELQLDVITAPLIRELTNAKLVEYGLSKIRRQHTRLGV
                     PLYDARQIIMSPNKENANVPHGPEATNLTLAEHIKKEFALLEVFSQNLADAHMRGDIH
                     LHDLGMVDRPYCSGQSIEYVKKFGLNLPNAISIAKPAKHPEVLIEQVVKFSAALQGNF
                     AGAIGWDAFNLFLAPYLTNVDDARMKQLAQILIFEFAQQAVARGGQSIFSDLNLYWEV
                     PDHFQNVEAIGPRGQFTGKVYGDYAEESQRFVNALFDVYLEGDAMGRPFFFPKPNVHM
                     TEKFFKTEGHEEFLHKICQTATEKGNTYFVFDRGGTAKISECCRLTFKLDDQDLNDAK
                     TPWKMRYSAMQNVTINLPRIAYEAHQDDAKLFQLLEDRVDMVAQAHLQKREFIGKLLG
                     MGKFGPLALLTMNLDGEPYYRFNKASFLVGMVGLNELVQYHTGEQMHESKDATKFGLK
                     VISVMKKCADELGSKYGIRMPLEQTPAESTAYRFAKLDMKYYPLQAPTVIRGNKSTGE
                     IYYTNSTYLNVGADVSAIDRVKMEGRFHPLIDAGSMTHVWLGESKPPA"
BASE COUNT          578 a          725 c          713 g          479 t
ORIGIN      
        1 gggaccttga accccgcctt gagcatgaac ttccgtgcat cggcaatctc ctcctccttc
       61 gcttgcactc cgggcgacag ccccaggaca ccggcgcgct ccagctcctc tttgaagatc
      121 ggcctcctga acaatctata cagggcatac agggtgagct ctccggatcg ctccggggag
      181 atggaccggg catgctcagc tatccggatg tagatgtcct tctccttgcc cttgacagcc
      241 gtatatacct tggcaggagg gacttccttc tccttcagga tgttgaggtc gctcagggcg
      301 cgaagatagc cggtcaggat gagccggtgc atatcgaacc ctcgcttctt cagctctttg
      361 gagagatagc ttatggacct gccctcctct gagagcattg agatgatcgt ctgcttaagg
      421 tccctatctg gatggaacat atggtaacag atatggtaac agatatggta atgatatagc
      481 ttttctcgcc cggtttatgg gcgttaattg ttaacagaac cagttcagaa attcatttaa
      541 acttaggtgc tgaatgtcat aatccccgag ttggatggat gcaataggac cttctaaata
      601 ggccctcctt tctaattcat gaacgaggag atcattgaga agaacgtgag acaatgaaaa
      661 gcgcagaggc cacccggaac gcaaatatgg atagggagtc aacagagata tctctcttcg
      721 tccgcacctc ggacgaggag atcaagaagt gggacaagag caggatattc gatgccctga
      781 tgagggagac ggacataagc gaggacgcgg cggccatcgt cgccagggag gtggagaaga
      841 tgatcggcga gctgcagctc gacgtcatca ccgctccgct gataagggaa ctcaccaacg
      901 ccaagctagt cgagtacggc ctgtccaaga tccgcaggca gcatacccgc ctcggcgttc
      961 cgctctatga cgctcgccag atcataatga gcccgaacaa ggagaatgcg aacgtccccc
     1021 acgggccgga ggccaccaac ctgaccctcg cggaacatat caagaaggag ttcgccctat
     1081 tggaggtatt cagccagaac ctcgccgacg cgcatatgcg aggggacatt cacctgcacg
     1141 accttggcat ggtcgaccgc ccctactgca gcgggcagtc catagagtat gtgaagaagt
     1201 tcggcctcaa ccttcccaac gccatctcca tagccaagcc cgccaagcat cccgaggtgc
     1261 tcatcgagca ggtggtcaag ttctccgccg cactccaggg gaacttcgcc ggtgccattg
     1321 gctgggacgc ttttaacctg ttcctggcgc cgtaccttac caatgtggac gacgcccgaa
     1381 tgaagcagct ggcccagata ttgatcttcg agttcgccca gcaggcggtg gcccgcggag
     1441 ggcagtccat cttcagcgac ctgaaccttt actgggaggt gccggaccat ttccagaacg
     1501 tggaggccat cgggcccagg gggcagttca ccggaaaggt ctatggggat tatgccgagg
     1561 agagccagcg gttcgtgaac gccctgttcg acgtctacct tgagggggat gcgatgggcc
     1621 ggccgttctt cttcccgaaa ccgaacgtgc acatgacgga gaagttcttc aagaccgaag
     1681 ggcacgagga gttcctgcac aagatctgcc agacggccac ggagaaggga aacacctact
     1741 ttgtcttcga tcgcggcggg accgcgaaga tctccgagtg ctgccggctg accttcaagc
     1801 tggacgacca ggacctcaac gatgcgaaga cgccttggaa gatgagatac tcggccatgc
     1861 agaacgtgac catcaacctt ccgcgcatcg cttacgaggc ccaccaggac gacgcgaagc
     1921 tcttccagct gctggaggac cgcgtcgaca tggtggccca ggcccacctg cagaagcgcg
     1981 aattcatcgg caagctgctg ggcatgggca agttcggtcc cctggcgctc ctgaccatga
     2041 acctggacgg cgaaccgtac taccgcttca acaaggcgtc cttcctcgtc gggatggtcg
     2101 ggctgaacga gctggtgcag taccataccg gagagcagat gcacgagagc aaggatgcca
     2161 ccaagttcgg tctcaaggtc atctcggtca tgaagaagtg cgccgacgag ctggggagca
     2221 agtatggcat caggatgcct ctggagcaga cgcctgccga aagcaccgcc taccgcttcg
     2281 ccaagctgga catgaagtat tacccgctgc aggcccccac cgtcatcagg ggcaacaaga
     2341 gcaccgggga gatctactac accaactcca cctatctcaa cgtgggagcg gacgtctcgg
     2401 ccatagaccg cgtcaagatg gagggcagat tccatccgct catcgacgct ggctccatga
     2461 cccatgtctg gctcggagag tccaagccgc ctgcg
//
LOCUS       DAOSYN010000199         2488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_32436_length_2488_cov_4.056720, whole genome shotgun sequence.
ACCESSION   DAOSYN010000199 DAOSYN010000000
VERSION     DAOSYN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32436_length_2488_cov_4.056720"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..703
                     /locus_tag="PLJ11_06900"
     CDS             212..703
                     /locus_tag="PLJ11_06900"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HOO04429.1"
                     /translation="MYLFKSAEEKVRNHALSRVEEGLEVMGFLLGEVFTHDGHEYTVV
                     RDVATTGLESTSVSVRFDPDGFEALFASLECARFRYVLVGWYHSHPGHGCFLSSTDIA
                     TQKGMFARPYHTALVVDPVRREMDAFCLVDGEVRRRKFLVYWDEYQNPYYGESVRSRK
                     MRA"
     gene            complement(704..1243)
                     /locus_tag="PLJ11_06905"
     CDS             complement(704..1243)
                     /locus_tag="PLJ11_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO04430.1"
                     /translation="MKETTVYILEEVKRTIERVDEAVIDEIVGTLASAPKIFIYGVGR
                     SGLVGEAFAARLVQIGLNVHFVGDTTTPIVEKGHVVLIISNTGETMSAVQTANIVRRI
                     GAVVIGLTGSAHSKLGIASNIVLELVQPRDEQKKRMAPLGTIFELSSMVILDSMVPLL
                     MDRLHQDEASLRRRHAIWV"
     gene            complement(1240..2229)
                     /locus_tag="PLJ11_06910"
     CDS             complement(1240..2229)
                     /locus_tag="PLJ11_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOO04431.1"
                     /translation="MKDGIEDVHGLGLNAMEVQMVRVNLMSRSPDEEEVGRTPRTLEG
                     DLVVEIHRRKGKKEVRITNLDEKIKEDDTLISLSSGLAHNYEELSKLGVMGSELDVAL
                     SMHTPYYIDLAGEGDLTRKSMDSIRWAGMMTQEMGGNMVVTHMGLYGNLSKREAFENI
                     ADRLGEIVAWWESNKLAPRLGLETSGRKEVFGGLDEILELCDRFDRLVPIVNFAHLHA
                     RNNGMLREPEDFAEVFDQVRSYVGDLNYTHFSGVEHEGGNERRVTPIKKGDLRFEPLA
                     EYLADANPNAVIISSSPLLEHDAMYMKVIYERVLTKRVSKGKKGKGIKDEDEE"
BASE COUNT          480 a          798 c          694 g          516 t
ORIGIN      
        1 agtacttcaa cgccacgccg aggaggacgc ccccgatcat cctctccccc acccccaagg
       61 tggtgcgcca atgaggaggc cgccggtcat cctcgagggg aaggacatgg agatcgagga
      121 gatgcagccc cccgccccgg aaaggacgct tccacataaa tggctgccca atgacgctaa
      181 gcctctctat tccgagccag ggggcgccgg gatgtacctt ttcaagtcag ctgaggagaa
      241 ggtgcggaac cacgccctct cccgggtgga agaagggctg gaggtcatgg gctttctgct
      301 gggagaggtc ttcacccacg atggccacga atacacggtg gtgcgggacg tggccaccac
      361 aggcctggag tccacctccg taagcgtccg cttcgatcca gatggcttcg aggccctgtt
      421 cgcctcattg gaatgcgctc gcttccgcta cgtcctggtc ggatggtacc actcccatcc
      481 gggccatggc tgcttcctct cctcaacgga catagccacc cagaagggca tgttcgcccg
      541 gccgtaccac accgccctgg tggtggaccc tgtgcggcgg gagatggacg ccttctgcct
      601 ggtcgacgga gaggtgcgca gacgcaagtt cctggtctac tgggatgagt accagaaccc
      661 ctactatggg gagagcgtga gatccaggaa gatgcgcgcc tgatcagacc cagatggcgt
      721 ggcggcgccg tagggatgcc tcgtcctgat ggagtctatc catgagaagg gggaccatcg
      781 aatccaggat gaccatgctg gacagctcga agatggtccc taggggcgcc atcctcttct
      841 tctgctcatc cctcggctgt acaagctcaa ggacgatatt gcttgcgatc ccaagcttcg
      901 aatgggcgct gccggtaagg cctatgacca cggcccctat cctccgcacg atgttggccg
      961 tctgcaccgc ggacatggtc tctccagtgt tcgagattat gaggacgaca tgccccttct
     1021 ccacgatggg cgtcgtggtg tccccaacga aatgaacgtt aaggccgatc tgcacaaggc
     1081 gcgcggcgaa ggcctcgccg accaggcctg acctgcccac cccgtagatg aagatcttgg
     1141 gggcggatgc cagcgttccc acgatctcgt cgatgaccgc ctcatccacc cgttcgatgg
     1201 tccgcttgac ctcctctaga atatagacgg tggtctcctt cactcctcat cctcgtcctt
     1261 gatgcccttg cccttctttc ccttggacac ccgcttggtc agcaccctct cgtatatgac
     1321 cttcatgtac atggcgtcat gctccagcag gggcgagctg gagatgatca ccgcattggg
     1381 attggcatcc gccaagtact cggccaaagg ctcgaagcgc agatcgcctt tcttaatagg
     1441 cgtcaccctg cgttcgttgc cgccctcgtg ctccaccccg gagaagtggg tgtagttgag
     1501 gtccccaaca tagctacgga cctggtcgaa gacctccgcg aagtcctccg gttcccggag
     1561 catgccattg tttcgggcgt gcaggtgcgc gaagttgact attggaacga gccgatcgaa
     1621 ccgatcgcac agttcaagga tctcatccaa gccgccgaag acctccttgc ggccactggt
     1681 ctccaggccc agtctggggg cgagcttgtt gctctcccac caggctacga tctcccccaa
     1741 acggtcggct atgttctcga aggcctcccg cttgctgagg ttcccgtaga ggcccatgtg
     1801 cgtcacgacc atgttgccgc ccatctcctg ggtcatcatt cccgcccagc ggatagagtc
     1861 catgctcttg cgggtgaggt ccccctcccc cgccaggtcg atgtagtagg gggtgtgcat
     1921 ggagagggcg acatccagct cgctccccat gacccccagc ttggaaagct cctcgtaatt
     1981 gtgcgccaac ccggaggaca ggctgatgag cgtatcatcc tccttgatct tctcgtcaag
     2041 gttggttatc cggacctcct tcttgccctt tcgacgatgg atctcgacca ccaggtctcc
     2101 ttccagcgtt ctgggcgtac ggccgacctc ctcctcgtcc ggagagcgtg acataaggtt
     2161 cacccgtacc atctggacct ccatggcgtt gaggcctaga ccgtgcacgt cctcgatgcc
     2221 gtccttcaaa gtacgaccct tacaagagag cggaatacca gctggaccaa agcgaatcat
     2281 cgataacccc gagcaaacta aacgatatgt ccaacgacat atataatgac tattggaaat
     2341 taatttaaag gcgttaatat tatctgtccc gcccttcgat caccgcatgc ggcatccgtg
     2401 caatggatgt acgcatggcc gctcgcgccg tcctccgcag ggcatggcca acaggccctg
     2461 ggccctcggt cccatggcac gcttcgat
//
LOCUS       DAOSYN010000200         2476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_32612_length_2476_cov_3.684841, whole genome shotgun sequence.
ACCESSION   DAOSYN010000200 DAOSYN010000000
VERSION     DAOSYN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32612_length_2476_cov_3.684841"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="PLJ11_06915"
     CDS             complement(<1..2000)
                     /locus_tag="PLJ11_06915"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04432.1"
                     /translation="MRPRPHPCIDIINGEMVLPIDYTDEDIAARWEEFLEAEENRLRL
                     LELTDRLPEMRSLTFTYSEIDRFDPDFASFILGHPDRALRKGEEVVRRLSDAARTTAE
                     VHLRIKELPRDSRIEIRNLRSEHLGRLISVEGLVRKATEVRPRLVLGVFRCMRCPARI
                     LEPQDGLYFREPMECYKEQEGCGRTAGSTKFQLLTEESVYIDTQKVEVQESPEGLRGG
                     AQPERLTAFLEDDLAGALSPGDRVVLNGVLRSQQKGDRIKSTLFDIDLAVLSTEFEQH
                     EYEEVVISEEDEEAIKREAKDPEVFQRIVGSIAPAIHGYEEVKESIALQLFGGTPKQL
                     EDGTKIRGDIHILLVGDPGVAKCVTGEAMVLMGDRTERNIQELVEHWLSVNGATEVED
                     GEYAEADFEVMTFTAQGRLEKGRAVRVWRRDSPRHLLRFTTEGGRTLTVTSTHPLFVQ
                     NAYFLAQRPAGKIAEGNYLAVASDDVPEGNYCGFDRGIGWDQVVRKEEVRAETPFVYD
                     IEVADTHIFVANGIISHNSQLLRYMSEMAPRGIYASGKSSSAAGLCVAPETLIEVDGR
                     AQPIGEFVEARMRSPIEVEAGVWRQEVRVNGVATAAGDRHTDVRPVISVFRLRTPSFL
                     VELRTEAGNTIRVTPETMVRAQRGGRPRWVKASDILAGDHVL"
     gene            complement(2038..>2476)
                     /locus_tag="PLJ11_06920"
     CDS             complement(2038..>2476)
                     /locus_tag="PLJ11_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HOO04433.1"
                     /translation="LRAAPYGLLVLDPFAQRAISHEDGARAREHGILVMDLSWENIER
                     FPKVRGDAAHRALPYLLAANPINWGRPLRLTSAEAVAAALYIVGEEGQAREVLSKFTW
                     GEQFLVLNREPLERYAAARTSQEVVQVQWDYCPPPEDGGRDEG"
BASE COUNT          352 a          869 c          804 g          451 t
ORIGIN      
        1 tggaggacgt ggtcgcctgc caaaatgtca gacgccttga cccatcgggg acggccccct
       61 ctttgggcgc ggaccatggt ctccggggtg acccggatgg tgttaccagc ctcggtgcgc
      121 agttccacaa gaaatgaagg ggtccgtagc cggaagaccg agatcaccgg tcggacgtcc
      181 gtatgacggt cccccgccgc cgtggctacc ccgttcaccc ggacctcctg ccgccagacc
      241 ccggcctcga cctcgatcgg ggagcgcatg cgcgcctcga cgaactcgcc gatgggctgt
      301 gccctcccgt ccacctcgat gagcgtttcc ggcgcgacgc acagcccggc ggcggatgag
      361 ctcttcccgg aggcgtagat cccgcggggg gccatctccg acatgtacct gaggagctgg
      421 ctgttgtgcg agatgatgcc gttcgccacg aagatgtgcg tgtcggccac ctcgatgtcg
      481 tatacgaacg gcgtctccgc ccttacctcc tccttcctca cgacctggtc ccagccgatg
      541 ccgcggtcga agccgcagta gttgccctcc gggacatcgt ccgacgccac tgccagatag
      601 ttgccctcgg cgatcttgcc ggcggggcgc tgcgccagga agtaggcgtt ctgcacgaag
      661 aggggatggg tggaggtgac ggtcagcgtc cgcccgccct cggtggtgaa ccggaggagg
      721 tgccgggggg agtccctcct ccagacgcgg accgccctgc ccttctccag ccgtccctgc
      781 gcggtgaacg tcatgacctc gaagtccgcc tcggcgtact ccccgtcctc cacctccgtt
      841 gccccgttga ccgagagcca atgctccacc agttcctgga tgttcctttc cgtgcggtcg
      901 cccatgagca ccatggcctc gccggtgacg cacttggcga ccccggggtc gccgacgagg
      961 aggatgtgga tgtcccccct gatcttcgtc ccgtcctcga gctgcttggg ggtgcccccg
     1021 aacagctgca gggcgatgct ctccttgacc tcctcatagc cgtggatggc cggggcgatg
     1081 gagcccacga tcctctggaa gacctcgggg tccttcgcct ccctctttat cgcctcctcg
     1141 tcctcctcgg agatgaccac ctcctcgtac tcgtgctgct cgaactccgt ggagagcacc
     1201 gcaaggtcga tgtcgaagag ggtggacttg atgcggtcgc ccttctgctg gctcctgagg
     1261 acgccgttca ggacgacgcg gtccccgggg gagagcgccc ccgccaggtc gtcctcgagg
     1321 aaggctgtga ggcgctcggg ctgggcccct cccctcagcc cctcggggga ctcctgcacc
     1381 tccaccttct gcgtgtcgat gtacacggac tcctccgtca gcagctggaa cttggtgctt
     1441 ccggccgtcc tcccgcaccc ctcctgctcc ttgtagcact ccatcggctc ccggaagtac
     1501 agcccgtcct gcggctccag gatccgggcg gggcagcgca tgcagcggaa gacgccgagg
     1561 acgagccgcg ggcgcacctc ggtggccttg cggaccagcc cctccaccga gatgagcctc
     1621 ccgaggtgct cgctcctcag gttgcggatc tcgatgcggc tgtccctggg gagctccttg
     1681 atgcgcaggt gcacctccgc ggtggtgcgg gcggcgtcgc tgagccgccg gaccacctcc
     1741 tcgccctttc gcagggcgcg gtccgggtgg cccaatatga agctggcgaa gtcggggtcg
     1801 aaccggtcga tctccgagta ggtgaaggtc aggctccgca tctcaggaag cctgtccgtg
     1861 agctcgagca gcctcagccg gttctcctcc gcctccagga actcctccca gcgcgcggct
     1921 atgtcctcgt cggtgtaatc gataggcaaa accatctccc cgttgatgat gtcgatgcag
     1981 ggatgcggcc ggggccgcat caaggaggtg gggtattccc ttgggccatt tttaccttta
     2041 cccttcgtcc cgcccgccgt cctcgggagg ggggcagtag tcccattgga cctggaccac
     2101 ctcctggctg gtgcgggcgg ccgcgtaccg ctcgaggggc tcgcggttca ggacaaggaa
     2161 ctgctcgccc caggtgaact tggagaggac ctcccgcgcc tgtccctcct ccccgacgat
     2221 gtagagggcg gcggccaccg cctccgcgct ggtgagccgc aggggcctcc cccagttgat
     2281 cgggttagcg gccagcagat aggggagggc gcggtgggcg gcgtccccgc gcaccttcgg
     2341 gaaccgctcg atgttctccc atgagaggtc catgaccagg atgccgtgtt ccctcgccct
     2401 cgccccgtcc tcgtgggaga tggccctctg ggcgaaggga tcgagcacca ggaggccgta
     2461 gggcgccgcc ctgagg
//
LOCUS       DAOSYN010000201         2427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_33429_length_2427_cov_4.178752, whole genome shotgun sequence.
ACCESSION   DAOSYN010000201 DAOSYN010000000
VERSION     DAOSYN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33429_length_2427_cov_4.178752"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..1173)
                     /locus_tag="PLJ11_06925"
     CDS             complement(376..1173)
                     /locus_tag="PLJ11_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO04434.1"
                     /translation="MPGEPIVEMRNVTVTKGSATLLRGMDLTVRQGERLVILGPNGSG
                     KSSLIKTMMGELRHDTSVPGAYVRLRGKDLWNLFDVRKAFGLVSADLQVDLARDIDCL
                     DAVLSGFFGSMGTNRSQEVDRGMRRRAMESLAAVGSAHLAGRRFHTLSTGEARRVLMA
                     RALVGSPEALVLDEPMSSLDLIGKRLVRQAIRSLAQEGRSLVLVTHDPSDIVPEIGRV
                     VMVKDGSVLRDGGLDLLTEENLTILYDGPIGLRRIEGMYFAWPRNGE"
     gene            complement(1431..2096)
                     /locus_tag="PLJ11_06930"
     CDS             complement(1431..2096)
                     /locus_tag="PLJ11_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOO04435.1"
                     /translation="MLYFEYGQKEIGHLRRRDMRLGEVIERMGMLNAELYPDLFTGLM
                     TSVVGQQVSGKAAATVMGRMCERFEITPEGLSKASPQDIRSCGTSLRKAEYIKGIADA
                     CLSGDLDLNALRSMTDEEVVRALRSLRGIGEWTAEMMLIFSLGRKDVVSWGDLAIRRG
                     MATLYGSEGLSREDFLRIRQTYSPYGTVASLYIWELDQERNGPGVSRKAVRRSSLSRG
                     PPM"
     gene            2243..>2427
                     /locus_tag="PLJ11_06935"
     CDS             2243..>2427
                     /locus_tag="PLJ11_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04436.1"
                     /translation="MHGNAVDGRGQRHPPFQSPGLAQWRKALRMGVPMRCRPLGGSLE
                     QARRLQLLGDLVDRCAHV"
BASE COUNT          376 a          813 c          763 g          475 t
ORIGIN      
        1 gcgccgcggg gcgccggtct gcgagagcgc gtccttgccg aggaggacct cgaccatctc
       61 gtcggggacg tcgaacgcct cgatatagac cgtctccccc tccttgaggc gaatggaatg
      121 gtcgatgagc acgtccgcca atttgcgcat cctagggtcg tacattggga cgcctaatcc
      181 cgaggagggt atatcagcgt tgggtaacta cgctgggagg ggcggcgggg gaccgctccc
      241 ggcatgcggc gcgtgggcct gtgccccttc gcagggccga tggctggagg ttggtggccg
      301 tgagggcgcc gcggtcgatg cgggcctttc tttaccttgg ggaaaatgat aggggacgag
      361 gttccgcctt ccgcctcatt ccccgttcct gggccaggcg aagtacatgc cctcgatcct
      421 gcggaggcct atcgggccgt cgtaaaggat ggtgaggttc tcctcggtga ggaggtccag
      481 ccctccgtcc cggagcacgc tcccgtcctt gaccatgacc acccgcccga tctcggggac
      541 gatgtccgag gggtcgtgcg tcaccagcac cagggagcgg ccctcctggg cgagggagcg
      601 gatggcctgc ctgaccagcc tcttcccgat caggtccagg gaactcatcg gctcgtccag
      661 gaccagggcc tccggggagc ccaccagggc ccgcgccata agcacccggc gcgcctcgcc
      721 tgtggatagt gtatggaagc gccggccggc cagatgggcg gagcctacgg ccgccaggct
      781 ctccatggcc cttcgcctca tcccccggtc cacctcctgc gagcggttgg tgcccatcga
      841 gccgaagaag ccggacagca cggcgtccag gcagtcgatg tccctggcga ggtcgacctg
      901 caggtcggcg gagacgagcc cgaaggcctt ccggacgtcg aagaggttcc agaggtcctt
      961 ccctctcagc ctgacatacg cgccgggcac ggacgtgtcg tgcctgagct cccccatcat
     1021 ggtcttgatc agggaggact ttccggagcc gttcgggccg aggatgacca gcctctcgcc
     1081 ctgccggacc gtgaggtcca tccccctgag gagcgtggcg ctgcccttgg tcacggtgac
     1141 gttccgcatc tcgacgatcg gttcccccgg cattcgggac cgtatcttcc agggaatata
     1201 aacgagatcg ctgaggcagg gccgcgcctg atgccttaga acataatgga gaatggggcc
     1261 gtcgtcctct taggagcggc ggagagcggc catcgcgggc cggaaggcct gaggaatgcc
     1321 tatttcttct tccccttcgc cggggcggcc ttgtcggcag cggccgcctt cttctcctcg
     1381 ttccttctct tctgcttctg caggtccttc ttggacatcg ccatgggccc tcacatcggc
     1441 ggacccctac ttaacgatga tcgtcgtacc gccttcctgg agaccccggg gccgttccgt
     1501 tcctggtcca gttcccaaat gtataacgag gcgaccgtgc catacgggga gtaggtctgc
     1561 cggatcctca ggaagtcctc cctgctcagc ccctccgatc cgtagagggt ggccatcccc
     1621 cggcggatgg caaggtcccc ccagctcacc acgtccttcc ggcccaggga gaagatgagc
     1681 atcatctccg cggtccattc ccctatcccg cggagggagc ggagggccct caccacctcc
     1741 tcgtccgtca tggaacggag ggcgttgagg tccaaatccc cactgaggca ggcgtccgct
     1801 attcccttta tatactcggc cttgcggagg gacgttccgc acgatcgtat gtcctggggg
     1861 ctggccttcg acagcccttc gggggtgatc tcgaagcgct cgcacatccg acccatgacc
     1921 gtggccgcgg ccttccctga gacctgttgg cccaccacgc tcgtcattag gcctgtgaat
     1981 aggtcggggt acagctcagc gttgagcatt cccatgcgct cgatgacctc gcccagcctc
     2041 atgtcccggc gtcgcagatg accgatctcc ttctggccgt actcaaagta aagcattggc
     2101 gtcccgcccc cgttccctgg gccgtgttcg ggagggcgtc cgtcgattcg aatagggaga
     2161 catccttcct tggtgctcaa ggccgcttcc aatagctatt tttgccatat aagagcgctg
     2221 tttgccggga tccgatccct tcatgcacgg gaacgccgtc gatggccgcg ggcagcggca
     2281 tccgcccttc cagagcccgg gtctcgccca atggcggaag gcccttcgta tgggcgttcc
     2341 gatgcgctgt cgtcctttgg gcggatcact tgagcaggcc cgccgccttc agctcctcgg
     2401 tgatcttgtc gaccgctgcg cccacgt
//
LOCUS       DAOSYN010000202         2427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_33432_length_2427_cov_3.543002, whole genome shotgun sequence.
ACCESSION   DAOSYN010000202 DAOSYN010000000
VERSION     DAOSYN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33432_length_2427_cov_3.543002"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1257)
                     /gene="hypE"
                     /locus_tag="PLJ11_06940"
     CDS             complement(211..1257)
                     /gene="hypE"
                     /locus_tag="PLJ11_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOO04437.1"
                     /translation="MKRVTMGHGAGGEMMQELIAKSIAPFLPKVPTEVPLASMDDSAV
                     VDDIVFTTDGHTVKPLFFPGGDIGSLAVSGTVNDIAVMGGRPLALSMAMVLEEGLEME
                     VLERVAQSIGRTSEIAGVPVVTGDTKVMEKGAMDGMVIAAAAVGRRHPSLDRNLEVAS
                     SYRKVDSRWLTDDNVRDGDAILVSGTLGDHGIALLSFREGYGFESKVQSDCAPLNHMI
                     GRLLEVGGVTSMKDATRGGFANAINEWCSKSKVGMELVEKDIPVSEAVRNASEMLGLD
                     PLTIGNEGKVIIACVPEMAEELLKALRADEHGRDAAIVGRASKAAKHVVLRTEVGGRR
                     ILEPPVGDPVPRIC"
     gene            1316..2044
                     /locus_tag="PLJ11_06945"
     CDS             1316..2044
                     /locus_tag="PLJ11_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04438.1"
                     /translation="MEMGDEGDLHREGRPLRVLCLGGGSICEAVMNHLASTNASFVVL
                     DTDPRCRVAGRCQVHEGLWEGPPALVIGDAIEGAMRLMEEHEVDLIVPAVPGHAMGML
                     TMAWGKGRLSPRGSMTVLDGLNRVLAGSGRAAMDEGNGTIIATLNTTALPCPLDCPQE
                     GPCPITGQPRKVDMGVVLEDVIARMGIAGVVIRPVRVGHFGAVTAEQARALRGLVEKL
                     RAGEVVAVATSCSCHAIMNVFKVV"
     gene            2102..2179
                     /locus_tag="PLJ11_06950"
     tRNA            2102..2179
                     /locus_tag="PLJ11_06950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2138..2140,aa:Arg,seq:tcg)
BASE COUNT          448 a          775 c          742 g          462 t
ORIGIN      
        1 ccggcagggg gctgcccttc cttatcaggg taatggccac cggttcatgc ctcaagccaa
       61 gcgtttcctt cagatcgttc gccatcgcag catagtccga cattggatct ccagtgagac
      121 actggagctg acggataata aattttatta tatatcaaat aaaaaattat atgccttccg
      181 gcctctgtca gattcaagac agcgtgctcg tcagcatatc ctcggcaccg gatcacccac
      241 gggcggttcc aatatgcgcc ttccccccac ctcggtcctc agcaccacat gcttggcggc
      301 cttggatgcc cggccgacga tggccgcgtc ccttccgtgc tcgtcggccc tcagggcctt
      361 cagcagctcc tcggccatct ccggcacgca ggcgatgatg accttgccct cgttcccgat
      421 ggtcagggga tcgaggccaa gcatctcgct ggcgttgcgc actgcctcag agaccggaat
      481 gtccttctcc acgagttcca tgcccacctt ggatttcgag caccactcgt tgatggcgtt
      541 agcgaatccc ccgcgggtgg cgtccttcat ggaggttaca ccacccactt ccaggagcct
      601 gccgatcata tggttgagcg gggcgcagtc gctctgcacc ttgctctcga agccatagcc
      661 ctcgcggaag gagagcaggg cgatgccatg gtcgcccaag gtgcccgaga cgaggatggc
      721 gtccccgtcc ctgacattat cgtcggtgag ccatctggaa tccacctttc ggtaggatga
      781 ggcgacctcg aggttgcggt ccagggacgg atgcctcctg cccacggcgg ccgccgcgat
      841 gaccatcccg tccatcgccc ccttctccat gaccttggta tccccagtca ccacggggac
      901 gccggcgatc tccgaggtcc ttccaatgct ctgggccacc cgctccagca cctccatctc
      961 cagcccctcc tcgaggacca tcgccatgga gagcgccaga ggcctccctc ccatcaccgc
     1021 gatgtcgttc accgtgccgc tgacggccag ggagccgatg tccccccccg ggaagaacag
     1081 gggcttgacg gtatggccat cggtggtgaa gacgatgtcg tccaccacgg ccgagtcgtc
     1141 catggaggcc agcgggacct cggtcggcac cttgggaaga aacggggcga tggacttcgc
     1201 gatgagctcc tgcatcatct ccccgcccgc cccatgcccc atggtgaccc gcttcattgg
     1261 gggtgcgaaa cggaaggcag attattaaga ttaccaaaga cgaataggat aagcggtgga
     1321 aatgggcgac gagggcgatc tccacaggga agggcggccc ctccgcgtgc tatgcttggg
     1381 tggggggtcg atatgcgagg cggttatgaa ccacctggcc tccaccaatg ccagcttcgt
     1441 ggtgctcgac acggacccgc ggtgcagggt ggccggccgt tgccaggtcc acgaagggct
     1501 ttgggagggc ccgccggccc tggtcatagg cgatgccatc gaaggggcga tgaggctcat
     1561 ggaggagcac gaggtcgatc tcatcgtccc cgccgttccg gggcacgcca tgggaatgct
     1621 gaccatggca tggggcaagg gaaggctgtc cccccggggc tcgatgaccg tcctcgacgg
     1681 cctgaacagg gtgctcgccg gctctgggag ggcggccatg gacgagggga acggaacgat
     1741 catagccacg ttgaacacca ccgcgctccc gtgcccgttg gactgccctc aggaaggccc
     1801 gtgtccaatc actggccagc ccaggaaggt ggacatgggg gtagtcctgg aggacgtcat
     1861 cgcccggatg gggatcgccg gggtggtcat ccggccggtc cgggtggggc atttcggcgc
     1921 ggtgaccgcc gagcaggccc gcgcccttcg agggctcgtg gagaagctga gagcgggcga
     1981 ggtggtggcc gtggccacca gctgctcctg ccacgccata atgaacgtgt tcaaggtcgt
     2041 ctgagccctc cggcgcccgg gccaatagcc ttatctccac ggccctcata ccgggaacga
     2101 cgggctcgta gggtagcctg gattatcctt tcggccttcg aagccgaaga ctggggttcg
     2161 aatccccacg ggcccgccat catgcatatc caatcgttga aaattattcg gctaatgacc
     2221 atggttcgat aagtattgcg ctccttgaac tccacgtctc ccgcaacgat cccatcttaa
     2281 cggccctgcc gaaggccacg ggcctcgtac cgttgtaaat aagtaatgga agcgcatttc
     2341 ccgtttcgtc cgagcttgct tggtgatagc agaaggccgg tcggaccccg aggaggttga
     2401 agcatggacc ccatggtact attgatc
//
LOCUS       DAOSYN010000203         2424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_33479_length_2424_cov_4.383284, whole genome shotgun sequence.
ACCESSION   DAOSYN010000203 DAOSYN010000000
VERSION     DAOSYN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33479_length_2424_cov_4.383284"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLJ11_06955"
     CDS             complement(<1..180)
                     /locus_tag="PLJ11_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04439.1"
                     /translation="MKKALCFTCLHRENRPELGVNDQLIYCRKKEMVVRPKGECPLYS
                     RATARAVQELNRALYG"
     gene            complement(215..1207)
                     /locus_tag="PLJ11_06960"
     CDS             complement(215..1207)
                     /locus_tag="PLJ11_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOO04440.1"
                     /translation="MTDHGCIKCGSCLRACPVLSDPNGTGFPGPRTVGVDVPRFPERG
                     RIGERALMCTMCHACEWACPSGLEITGAMLQLRTEVSPGLQLPGHLRMRENVRSFGRA
                     VRTEFTGWREEGNRTLYFPGCIGLGPLAAMTTTASSLLTAFGIEHDISSRAVCCGSPL
                     LKVGALAEAKALQHRNMETLAAYERVITSCPGCTYQLREVYGVRAEHMVEVLKAKDLR
                     SKVRGKWALQLPCHLKRGLSPWSAEALVHVLTGAGVDIVRLPVEDRCCGGGGGMLAGF
                     PATARSMAHNKADAYRAAGVEGVITACPFCALNLGREGLRVLDVSEAVHAEKMR"
     gene            complement(1204..2253)
                     /locus_tag="PLJ11_06965"
     CDS             complement(1204..2253)
                     /locus_tag="PLJ11_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOO04441.1"
                     /translation="MNKALVVGSGAAGLFSSWLLARDGWPVIVVGKGTPSSAMSTGCL
                     RRMPEACHEKALNFLADEGMPMVTGERTGISKLGTPFECWLSPSHSTWSPEGAPGSVT
                     VVGFQGHPSLHPNLISSVLGHRGIRARPLTLPSTLPPDIPLTALFRDEGAWEGVATEV
                     QRTEGGTVLLPAFLSRRDYWRLDWLERRCGRTVLEAVTPLEAPGQRLLDLMRARAIEA
                     GASIWDGRTVTSLTVENGSVRTAIVRGGLEVREMEVGALVMATGGALVNGLGLNGREL
                     ADPFTAFKVLDDGDVLRGGYATEKGRLVSRSGNVVRNAAGAGDCVRAPCKGYGDGLPH
                     AFESAWTAVKALEGA"
BASE COUNT          386 a          883 c          732 g          423 t
ORIGIN      
        1 cccgtacagg gcgcggttga gctcctgcac cgcacgggcg gtcgctctgg agtacaacgg
       61 gcattccccc ttcggacgga ccaccatctc cttcttgcga cagtagatca gctgatcgtt
      121 gacccctagc tcaggcctgt tctcacgatg cagacatgta aaacatagcg ctttcttcat
      181 ccaggccgtt ctaatgtgca ttctatattt gatgctatct catcttttca gcgtgaacgg
      241 cctcgctgac gtccaggacc ctgagcccct cccgccccag gttgagggcg cagaacgggc
      301 aggccgtgat gaccccctct acccctgcgg cgcggtatgc gtccgccttg ttatgggcca
      361 ttgacctggc ggtggctggg aagccggcga gcatccctcc ccctccgccg cagcagcggt
      421 cctccaccgg gaggcggacg atgtccaccc ccgccccagt caggacatgc accagggcct
      481 cggcgctcca cgggctgagc ccccttttga gatgacaagg gagctggagc gcccattttc
      541 ccctcacctt ggaacggaga tccttggcct taaggacctc caccatgtgc tccgccctca
      601 ccccgtagac ctcccgaagc tgataggtgc agccagggca ggaggtgatc acgcgctcgt
      661 aggccgccag cgtctccatg ttgcggtgct ggagggcctt ggcctccgcc aacgcgccca
      721 ccttgaggag cggtgagccg cagcagaccg ccctgctcga tatgtcgtgc tcgatgccga
      781 aggccgtcag cagggacgag gccgtggtgg tcatggcagc gagggggccg aggccgatac
      841 agcccgggaa gtacagcgtg cgattgcctt cctcccgcca gcctgtgaac tccgtgcgaa
      901 cggcgcggcc gaaggaacgg acgttctccc tcatccggag atgcccaggc agctggaggc
      961 cgggggagac ctccgtcctg agctggagca tggcccctgt gatctccagt ccggaggggc
     1021 aggcccattc gcatgcatgg cacatggtgc acatcagcgc ccgctccccg atcctccccc
     1081 gctcagggaa gcgcgggacg tccaccccga ccgtcctcgg cccagggaac cccgtcccgt
     1141 tcgggtcgct caggactggg cacgcccgca ggcagctgcc gcatttgatg catccgtgat
     1201 cggtcatgcg ccctccaggg ccttgaccgc cgtccaggcg ctctcgaatg cgtgggggag
     1261 cccatctccg tagcccttgc atggggcgcg gacgcagtcc ccggcccctg cggcgttcct
     1321 gaccacgttc cctgacctgg agacaagcct gcctttctcg gtcgcgtatc cgcccctcag
     1381 gacgtcaccg tcgtccagca ccttgaaggc ggtgaacggg tccgcaagct ccctcccgtt
     1441 caggccgagc ccgttcacca acgccccgcc ggtggccatg accaacgccc ccacctccat
     1501 ttcccggacc tcgaggccgc cccgcacaat ggcggtgcgg accgagccgt tctcgaccgt
     1561 cagggacgtg accgtccttc cgtcccagat actggccccg gcctcgatcg ccctggcgcg
     1621 catgagatcc aggagccttt ggcccggggc ctccaggggg gtgaccgcct ccagcaccgt
     1681 ccttccgcac cggcgctcca gccagtccaa tctccaatag tcccgtctcg aaaggaacgc
     1741 ggggaggagc accgtcccgc cctcggtcct ttgcacttcg gtcgccactc cctcccacgc
     1801 cccctcgtcc ctgaagagtg cggtcagggg gatgtccggg gggagggtcg aggggagcgt
     1861 cagcggcctt gcccttatcc cccgatggcc gaggacggag gagatgaggt tcggatgcag
     1921 ggagggatgc ccctggaagc cgacgaccgt cacgctgcct ggcgcccctt cggggctcca
     1981 tgtggaatga gagggcgata gccagcattc aaaaggggtc ccgagcttac ttatcccggt
     2041 gcgctccccc gtcaccatcg gcatcccctc gtcggccagg aagttgaggg ccttctcatg
     2101 gcacgcctct ggcatcctcc ttaggcagcc ggtggacatg gcggaggacg gggtgccctt
     2161 ccctaccacg atgaccggcc agccatccct ggccagcagc caggaggaga acagcccggc
     2221 ggccccgctc ccgaccacga gcgctttgtt cattccactc ctcccaggcc atacacgccc
     2281 cgcagcacgt gcctgcggag gacctcctgc cgtagctggt cgccgctggc caatgcttgc
     2341 cccctttccc gttcttccag gaactcggtc agggaggcca acggctccgt ccctccggcc
     2401 tccatcatca tctcttgaag ggag
//
LOCUS       DAOSYN010000204         2414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_33673_length_2414_cov_2.725307, whole genome shotgun sequence.
ACCESSION   DAOSYN010000204 DAOSYN010000000
VERSION     DAOSYN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33673_length_2414_cov_2.725307"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLJ11_06970"
     CDS             <1..309
                     /locus_tag="PLJ11_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HOO04442.1"
                     /translation="IGKKIGDTIDGIFVGLPGYKLTITGGSDKDGFAMRKDLPGPRRK
                     RLLLTTSTGFYTTEGGLRRRKSVRGHAIATDTVQINMKIISQGPKPVEELIKKEEKKK
                     "
     gene            306..1535
                     /locus_tag="PLJ11_06975"
     CDS             306..1535
                     /locus_tag="PLJ11_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HOO04443.1"
                     /translation="MKVSRQPEINIGMIGHVDHGKTTLTKALSGDWTDRHSEEIKRGI
                     SIRLGYADAAFYKCKRCEDAYSNSPVCKVCGGEAEFVRSVSFVDAPGHETLMATMLSG
                     AAMMDGALLLVAANEECPQPQTKEHLMALSIIGVERIIIVQNKIDIVTREEAMENYNQ
                     IRQFIKGTIAEGAPIIPVSAHHDVNIDKLIGAIQKYIPTKEHDERKPARMFVARSFDI
                     NQPGMSLEELKGGVLGGTLIQGVLSVGDEIEISPGRKVEQPGGKFTWETITTTIESLQ
                     TGGGFVDRIKPGGLVAIGTKLDPSLTKSDGLTGRVVGKPGTLPQVHFKFRMNTHLLDR
                     VVGTSEDLVVDNIKTNEPLMLSIGTATTVGLVTSARGDQSEVALKIPVCAEEGQRVAI
                     SRKIANKWRLIGYGIVV"
     gene            1542..1895
                     /locus_tag="PLJ11_06980"
     CDS             1542..1895
                     /locus_tag="PLJ11_06980"
                     /inference="COORDINATES: protein motif:HMM:NF037434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HOO04444.1"
                     /translation="MQKVVLDTNALLMPFEFKMNLDLELAHLFGEFEAYVPGPVIGEL
                     KRSGSKHAKAALMLAGKYKRFETSTQGDAGVLEAAKALDAMVVTNDIILRRKMRKEGV
                     HAVLLRSRRHLVIEE"
     gene            complement(1896..>2414)
                     /locus_tag="PLJ11_06985"
     CDS             complement(1896..>2414)
                     /locus_tag="PLJ11_06985"
                     /inference="COORDINATES: protein motif:HMM:NF021398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HOO04445.1"
                     /translation="GSGTAVVDDPELIQGIGGRLSGLVETSPIQSVLAEIGRDRVLDP
                     ETARIDQVAGARKAWAMGYRKIGVTVVKGADAEALRREFGDHVVLFAVHTSGVTEEEA
                     EALFAHCDIATACASKHMWALGKRLGAMQVGTKVPVFAITELGREICTVRLRQIGKEA
                     RSGPDCPARPLI"
BASE COUNT          481 a          789 c          773 g          371 t
ORIGIN      
        1 atcgggaaga agatcgggga caccatcgat ggcatattcg tagggctgcc cggctacaag
       61 ctgacgatca ctggcggaag cgacaaggac ggcttcgcca tgaggaagga cctgccaggc
      121 ccgcgccgga agaggctgct gctgaccacc agcaccggct tctacaccac cgagggcggc
      181 ctcaggagga ggaagtccgt ccgggggcat gccatcgcca ccgacacggt ccagatcaat
      241 atgaagatca tctcccaggg ccccaagccg gtcgaggagc tgatcaagaa agaggagaag
      301 aagaaatgaa ggtgtcccgc cagcctgaga tcaacatcgg gatgatcggc cacgtggacc
      361 acggcaagac cacgctcacc aaggccctct ccggtgattg gaccgaccgc cattcggagg
      421 agatcaagcg aggcatctcc atcaggctgg ggtacgcgga cgcggccttc tacaagtgca
      481 agcggtgcga ggacgcctac tccaactccc ccgtgtgcaa ggtgtgcggc ggggaggccg
      541 agttcgtccg ctccgtgtcc ttcgtggacg cccccgggca cgagaccctg atggccacca
      601 tgctgtcggg cgcggccatg atggacgggg cgctcctgct ggtcgccgcc aacgaggaat
      661 gcccccagcc ccagaccaag gagcatctca tggccctctc catcatcggc gtggagagga
      721 tcatcatcgt gcagaacaag atagacatag tcacccgcga ggaggccatg gagaactaca
      781 accagatcag gcagttcatc aagggcacca tcgcagaggg cgcgccgatc atcccggtct
      841 ccgcccatca cgacgtcaac atagacaagc tcatcggcgc catccagaag tacatcccga
      901 ccaaggagca tgacgaaagg aagccggccc ggatgttcgt ggcccggtcc ttcgacatca
      961 accagccggg gatgtccctg gaggagctca aggggggcgt gctcggcggc accctgatcc
     1021 agggcgtgct gagcgtaggc gacgagatcg agatctcccc cgggcgaaag gtggagcagc
     1081 ccggcggcaa gttcacctgg gagacgataa caaccaccat cgagtcgttg cagacgggcg
     1141 gagggttcgt ggacaggatc aagcccggcg ggctggtggc catcggtacg aagctggatc
     1201 cctccctcac caagtccgac gggctcaccg gaagggtggt gggcaagccc ggcaccctcc
     1261 ctcaggtcca tttcaagttc aggatgaaca cccatctcct ggaccgcgtt gtggggacca
     1321 gcgaggacct ggtcgtcgac aacatcaaga cgaacgagcc cttgatgctg agcatcggga
     1381 cggccaccac cgtggggctg gtcaccagcg ctcgcggcga tcagtccgag gtcgccctca
     1441 agatcccggt ctgcgccgag gaggggcagc gcgtggcgat ctcccgcaag atcgccaaca
     1501 aatggcgcct catcggctac ggcatcgtcg tgtgaggggc catgcagaag gtggtcctgg
     1561 acaccaacgc gctcctcatg cccttcgagt tcaagatgaa cctggacctt gagcttgccc
     1621 accttttcgg ggagttcgag gcgtacgtcc ccggccctgt gatcggggaa ctcaagcgct
     1681 cggggagcaa gcatgccaag gccgccctga tgctcgccgg caagtacaag aggttcgaga
     1741 cgtccactca gggcgacgcg ggcgtgctgg aggccgcaaa ggccctggac gcgatggtgg
     1801 tcaccaatga catcatcctg cggcggaaga tgaggaagga gggcgtgcat gccgtgctcc
     1861 tccgctcccg acggcacctg gtcatcgagg aatgatcaga tgagcggccg ggccgggcag
     1921 tcagggccgc tcctcgcctc cttgccgatc tgcctcagcc ggacggtgca gatctccctc
     1981 cccagctcgg ttatcgcgaa cacaggcacc ttagtgccga cctgcatggc gcccagccgc
     2041 tttcccaggg cccacatgtg cttggaggcg caggcggtgg cgatgtcgca gtgggcgaag
     2101 agcgcctcgg cctcctcctc ggtcaccccc gaggtgtgca ccgcgaagag cacgacgtgg
     2161 tccccgaact ccctgcgcaa tgcctcggcg tccgcgccct tgaccaccgt gacccctatc
     2221 ttcctatagc ccatggccca cgccttgcgg gcgccggcca cctggtctat gcgggccgtc
     2281 tcggggtcca gcacccggtc gcggccgatc tccgcgagca cgctttggat cgggctcgtc
     2341 tccacaagcc cggagagcct tcctccgatg ccctggatga gctccgggtc atcgacgacg
     2401 gccgtaccgg agcc
//
LOCUS       DAOSYN010000205         2409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_33757_length_2409_cov_3.999575, whole genome shotgun sequence.
ACCESSION   DAOSYN010000205 DAOSYN010000000
VERSION     DAOSYN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33757_length_2409_cov_3.999575"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..2101)
                     /gene="lonB"
                     /locus_tag="PLJ11_06990"
     CDS             complement(140..2101)
                     /gene="lonB"
                     /locus_tag="PLJ11_06990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOO04446.1"
                     /translation="MAEEGDERSAEIPDVETWIEGQAFTTTADIKIPEKLSDQVIGQD
                     SAVEVVRKAAEQKRHVMLIGEPGTGKSMLAKAMTEYLPKDELQDVIAYHNPEDPNEPR
                     IRTVPAGKGKEIVAAQKREALAKKSQKASMVTAIVMMVVVLSIILYIQTPDPMILLFG
                     IMAAAMIFFATRYVGQRQENYMIPKLLISHEEGATIPFIDATGAHAGALLGDVKHDPF
                     QSGGLETPAHERLEVGAIHKASKGVLFVDEINMLRMESQQSLLTALQEGKFSITGQSE
                     RSSGAMVKSEPVPCDFILVCAGNMDAIQGMHPALRSRIRGYGYEVYMRSTMDDTDENR
                     TKLVRFVAQEVAKDKKIPHFDRRAVMEIIKEAQRRAGRRGQLTLRLRELGGLIRVAGD
                     MAREESAPIVTQEHVIKAKKVARSLEQQIVDRYIQAHKLYPTFTTEGGAVGVVNGLAA
                     MNSQSSMSELSGIVLPIVAEVTPAQTKNGGQIIATGKLGEIAKEAVQNVSAIIKKYTG
                     EDITNHDVHVQFIGTYDGVEGDSASISIATAVISALEEVPVDQSVAMTGSLSVRGQVL
                     PVGGVTAKIEAAAESGLKKVLIPKANQYDVVLEERYIGKIEIVPVTNMGEVLSHALVS
                     GSKKDGLLRKLAALVERSALKPAAKPAAP"
BASE COUNT          365 a          786 c          765 g          493 t
ORIGIN      
        1 gcgccccttc aatccggttc aggtgataaa atccttgtca ttgggaaggg gggaggaagg
       61 ggtgcagagg gataggtgct ggcttactaa atggggatag gggaaagtgg aaaaggggga
      121 aaggggttgg ggagggcgtt caaggggcgg ccggcttggc cgcgggcttc agggcgctcc
      181 gctccacgag cgcggcgagc ttcctcagca ggccgtcctt cttgctcccg ctcaccaggg
      241 cgtgggagag cacctcgccc atgttcgtca ccggcacgat ctcgatcttg ccgatgtagc
      301 gctcctccag caccacgtcg tactggttcg ccttggggat gagcaccttc ttcagcccgg
      361 actccgcggc ggcctcgatc ttggccgtca cgccgccgac cgggagcacc tggccgcgga
      421 cgctcagcga gccggtcatg gccaccgact ggtccaccgg cacctcctcc agggcggaga
      481 tcaccgccgt ggcgatggag atggaggcgg agtcgccctc cacgccgtcg tacgtcccta
      541 tgaactgcac gtgcacgtcg tggttggtga tgtcctcgcc cgtgtacttc tttattatgg
      601 cggaaacgtt ctgcaccgcc tccttggcga tctccccgag cttgcccgtc gcgattatct
      661 gcccgccgtt cttcgtctgc gccggcgtga cctcggccac gatcgggagc acgatgccgg
      721 acagctccga catgctggac tgggagttca tcgccgcgag gccgttcacc acgccgacgg
      781 cgcccccctc cgtggtgaag gtgggataca gcttgtgcgc ctggatgtag cggtccacga
      841 tctgctgctc cagggagcgc gccaccttct tggccttgat cacgtgctcc tgggtgacga
      901 tcggggccga ctcctcgcgg gccatgtccc cggccacccg gatgagaccg cccagttcgc
      961 gcaggcggag ggtcagctgc ccgcgcctgc cggcgcgccg ctgcgcctcc ttgatgatct
     1021 ccatgaccgc cctcctgtcg aagtggggga tcttcttgtc cttggcgacc tcctgggcca
     1081 cgaaccgcac cagcttggtg cggttctcgt ccgtgtcgtc catggtggag cgcatgtaca
     1141 cctcgtaccc gtacccgcgg atgcgggagc ggagggcggg gtgcatcccc tggatcgcgt
     1201 ccatgttccc cgcgcacacg aggatgaagt cgcaggggac cggctctgac ttcaccatcg
     1261 cccccgagct cctctcggac tgcccggtga tgctgaactt gccctcctgc agcgcggtga
     1321 ggagcgactg ctgggactcc atcctcagca tgttgatctc gtcgacgaag aggacgccct
     1381 tggacgcctt gtggatggcg ccgacctcca gccgctcgtg ggcaggggtc tccaggccgc
     1441 ccgactggaa cgggtcgtgc ttgacatccc ccagcagcgc gccggcgtgg gcgccggtgg
     1501 cgtcgatgaa cgggatggtg gccccctcct cgtgggagat cagcagcttg gggatcatgt
     1561 agttctcctg ccgctgcccg acgtagcggg tggcgaagaa gatcatcgcc gcggccatga
     1621 tgccgaacag caggatcatc gggtccgggg tctggatgta caggatgatg cttaggacca
     1681 ccaccatcat gacgatggcg gtgaccatag aggccttctg ggacttcttg gcgagggcct
     1741 cccgcttctg ggcggcgacg atctccttgc ccttcccggc gggcaccgtc cggatgcgcg
     1801 gctcgttcgg gtcctctgga ttgtgatagg cgatgacgtc ctgcagctcg tccttgggca
     1861 ggtattccgt catggccttg gcgagcatcg acttgcccgt ccccggctcc ccgatgagca
     1921 tcacgtggcg cttctgctct gcagccttgc gcacgacctc gaccgcggaa tcctggccga
     1981 tgacctggtc ggacagcttc tccgggatct tgatgtcggc ggtggtcgtg aacgcctggc
     2041 cctcaatcca ggtctccacg tcagggatct ccgccgaccg ttcatctcct tcttctgcca
     2101 tgcttcacct tatgctcagg tcgaacccat gcagggactg ctaaataaag ctattcctct
     2161 ggaacagcgg aacctgcaac aggccagctt aacactccgt tcataattag gcgttccgtg
     2221 ccctttaaat gttcgccccc ggtatcgcgc gatcgggcgg ccgggggcat cccggccctt
     2281 ttcccccatt tcctcttctt caaggagggg cgggcacggc ggccggcccg gacgctggca
     2341 tcgcttcagc atcgatcact gatgatcgtt cacatgccag ttgtcatttt tcagctgtta
     2401 tcagtgata
//
LOCUS       DAOSYN010000206         2387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_34153_length_2387_cov_4.239708, whole genome shotgun sequence.
ACCESSION   DAOSYN010000206 DAOSYN010000000
VERSION     DAOSYN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34153_length_2387_cov_4.239708"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="PLJ11_06995"
     CDS             complement(<1..1617)
                     /locus_tag="PLJ11_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04447.1"
                     /translation="MTEGGLSTPCRKGVSVLFFFLMITSGLVLATSPASVQAEPPLVR
                     DEYEWGTDVLDHVGNMGLYNSLAVDDDGTLHAVYYDATNNDLKYATNPGGAWNITTID
                     SAGDVGAYCSVALDGRGDVHISYRDGTEGTLVHLTNSGGAWQREVVEAASAQYTDIAV
                     DASDAAAISYFDEDSFSLKYAFDAGSSWTIQTVDTGNVGAYSSLAIGHDGVARIAYYD
                     LANHDLKFANNTGGYWSSYTVDTVGDVGMHASLALDRAGEPYIAYYDLTNTNPKWAWN
                     DGTWHTQTLYAGPDVGQYTCIAIGANGEAHVSFQDLSGFALRYMSNQYGNWAPNLFGT
                     EPSGWTSMVVDASGRTHIIYYEFNSGDLKYVTDGTWFKELIKLFGDVAPYSSLVIDDQ
                     GNRHCCYVKAGTYDLYYANDTSGQWIRQLVEGGAGQEAVTLDMAVDSKGFAHICYYER
                     INGDLRYLNNTGGSWSNWTFTQDIVGWSPSIALDANDKAYISFYDLTDGGLMYATNRD
                     GVWAAAGLDYTGMSGFYSSLAVDDQGYMHVA"
     gene            2021..2314
                     /locus_tag="PLJ11_07000"
     CDS             2021..2314
                     /locus_tag="PLJ11_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04448.1"
                     /translation="MAIALLIATNGYWVLADGGLGSGLLGSDDEFEYLELVRLDSTRS
                     DYYQTLRENLCPSDPTYEEGVQFCTDLVRHDLGDTYWLTYDSSYYQATGGHAY"
BASE COUNT          474 a          688 c          737 g          488 t
ORIGIN      
        1 ggcgacgtgc atatatccct ggtcgtcaac cgcaagggac gagtagaagc cggacatccc
       61 ggtatagtcc agacccgccg ccgcccagac cccgtcccgg ttggtcgcgt acatcagacc
      121 gccgtcggtg aggtcataga aggagatgta ggccttgtca ttggcgtcca gggcgatgga
      181 gggggaccac cctacgatat cctgggtgaa tgtccagttg gaccagctcc ctccagtgtt
      241 gttcagatag cgcaggtcgc cgttgatcct ttcgtaatag cagatgtgcg cgaacccctt
      301 ggagtccacg gccatgtcca aagtgaccgc ctcctggccc gcgccgccct ccaccagttg
      361 gcggatccac tgccccgatg tgtcgttggc gtagtacagg tcgtacgttc cggccttcac
      421 gtagcagcaa tgccggttgc cctgatcatc gatgaccagc gagctgtaag gggcgacgtc
      481 cccgaacagc ttgatcagct ccttgaacca ggtcccgtca gtgacgtact tgagatcacc
      541 ggagttgaac tcgtaataga tgatgtgcgt ccgcccggag gcgtccacga ccatcgacgt
      601 ccagccggaa ggctccgtcc cgaagagatt gggcgcccag ttgccgtact ggttgctcat
      661 gtaccgcagg gcgaagccgc tgaggtcctg gaaggagacg tgggcttccc cgttcgcacc
      721 gatggcgatg catgtgtatt gaccgacatc cggtccggcg tatagggtct gggtgtgcca
      781 cgttccatcg ttccaggccc acttggggtt ggtgttggtg aggtcgtaat aggcgatata
      841 gggctctccg gccctgtcca gggccaggga cgcatgcata ccgacatccc cgacggtatc
      901 caccgtataa gatgaccagt agcccccggt gttgttggcg aacttgaggt cgtggttcgc
      961 caggtcgtaa taggcgatgc gggcgacacc gtcgtggcct atggccaatg atgaataggc
     1021 cccgacgttc ccggtgtcga cggtctggat cgtccaagag ctgccggcat cgaacgcgta
     1081 cttcaggctg aagctgtcct cgtcaaagta ggagattgcc gccgcatccg aggcatccac
     1141 ggcgatgtcg gtgtactgag cggacgccgc ctcgacgacc tccctctgcc aggccccgcc
     1201 ggagttggta agatggacca gggtcccctc ggttccgtcg cggtaggata tgtgaacatc
     1261 ccccctaccg tccagggcca cggagcagta ggcccccacg tccccggcgg aatcgatggt
     1321 ggtgatgttc caggcgcctc cgggattggt ggcgtacttg aggtcattgt tagtcgcgtc
     1381 gtaatagacc gcatgcaaag tgccgtcatc gtccacggcc agggaattat agagtcccat
     1441 gttccccacg tggtccagca catcggtccc ccattcatac tcgtcacgga ccagaggggg
     1501 ttctgcctgc acggaggcgg gggaagtggc caataccaag cctgacgtga tcatcaagaa
     1561 gaagaatagc accgagaccc ccttccggca aggggtggag agaccgcctt ccgtcatgga
     1621 tgatactact ctcgggatgg ggtattataa gtaatggtgt tggggcattt tcacgctggg
     1681 gatgtgatct cgttccatcg ctatccatat gcggccgagg tcggctgacg gttagtcagt
     1741 cagatggtgg tgattgactg cggtatagcg ttcgcacgga gaagggtcga ggtcttggtg
     1801 atgacccatt ccaacgtagg tccaataaaa atgagaacga tagtaattaa tcgttggatt
     1861 cgctcgagtt catgagatcg ccatgagcgc caatcccgga aataactttg gcccggacca
     1921 gggtcaatcg cagtatccgc cggttcaata caatggccag ccccagccac cttatgtccc
     1981 gcaggggagg aagcgcagcc cgtggctggc ggtcgcctgc gtggccatag ccctcctgat
     2041 agccaccaac ggctactggg tcctggcgga cgggggcttg gggagcggcc tcctagggag
     2101 cgatgatgag ttcgaatatc tggaactggt ccgcttggac agcaccaggt cggactacta
     2161 ccagaccctc agggagaacc tgtgcccttc cgatcccact tacgaggagg gcgtccagtt
     2221 ctgtacggac ctggtgaggc acgacctggg cgatacctat tggctgacct atgactccag
     2281 ctattaccag gccaccggag gccacgccta ctaggaggcg tcggaggaga tcgatgccat
     2341 ccttgatctc atcggcgtct cggattcgaa cgaccaggtg gagaact
//
LOCUS       DAOSYN010000207         2381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_34266_length_2381_cov_3.443680, whole genome shotgun sequence.
ACCESSION   DAOSYN010000207 DAOSYN010000000
VERSION     DAOSYN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34266_length_2381_cov_3.443680"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLJ11_07005"
     CDS             <1..305
                     /locus_tag="PLJ11_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04449.1"
                     /translation="RQEREGPRLFGQDERELYDTIYPPKEKGKKGPTDELQGRRCLFH
                     GALAVSACPNCNSMLCKECQAGGKCPRCGLVLGEKAHKEEEPPAPAEPEERDWSRL"
     gene            complement(306..1607)
                     /gene="truD"
                     /locus_tag="PLJ11_07010"
     CDS             complement(306..1607)
                     /gene="truD"
                     /locus_tag="PLJ11_07010"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOO04450.1"
                     /translation="MVRPSAQREREIGLEVFYTDTPGTGGKLKACPEDFVVEEVSKYP
                     PEKTDGRYCVARVTATNWETNRLVRHMAKALGISRNGIGFAGTKDKRAVTTQLMSFEA
                     PVERVMALRIPQVRVEGGYMARRGMALGDLVGNTFKIYVRDTELRGSELRSSLEGTQE
                     ALRELGGFPNFFGVQRFGAVRPVTHLVGRAIVKGDMERAVLLYIGNPSEDEEGPSREA
                     REALERDRDYSAALRTFPRTLTFERMVIGYLERNPEDYAGAIRVLPPNLQMMFVHAYQ
                     SYLFNVILSERMRRGIPLNAPIVGDVVLPADRDGNPDHDKPVPVTTANLDLVEEQVRE
                     RRAFVSATLFGYGSELAEGEMGEIERRAIEREGLSPRDFLVPQVPHCSSKGSRRELVC
                     EYRDLRLEVGEDGYVATFFLGKGCYATVLLRELMKADVCAY"
     gene            complement(1613..1888)
                     /locus_tag="PLJ11_07015"
     CDS             complement(1613..1888)
                     /locus_tag="PLJ11_07015"
                     /inference="COORDINATES: protein motif:HMM:NF025040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpoL/Rpb11 RNA polymerase subunit family
                     protein"
                     /protein_id="HOO04451.1"
                     /translation="MEIELLEKEKDSIKVRIKGADMTLITPMVQGLLEDKDVAEVKYT
                     IGHSQMEDPTLFVRVRTGKPQSALKKTAKALANEFKEAREALLKDLK"
     gene            complement(1930..>2381)
                     /locus_tag="PLJ11_07020"
     CDS             complement(1930..>2381)
                     /locus_tag="PLJ11_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04452.1"
                     /translation="ERSLDVQYYIWHGDLTGTLLMQALGQAAGRGVRVRLLLDDNNTT
                     GMDATLAALPEMLANDRLAARHHLVDSKMEADFWFQPRLVLEVRGAEITLSPIHTCCF
                     GAIRPDAGLAIRFPRFTGRFREDKEGRDATTSQEILAMYQAQLKRVE"
BASE COUNT          378 a          830 c          766 g          407 t
ORIGIN      
        1 accggcagga gcgggagggg ccgcgcctct tcggacagga cgaacgggag ctatacgaca
       61 cgatctaccc ccccaaggag aaggggaaga aggggccgac cgacgaactc caggggcgca
      121 gatgcctgtt ccacggcgcg ctcgccgtct cggcgtgccc gaactgcaac agcatgctct
      181 gcaaggagtg ccaggcaggg gggaaatgcc cacggtgcgg gctagtcctc ggggaaaagg
      241 cgcacaagga ggaagagcct cccgcccccg cggaaccgga ggaacgggac tggagccggc
      301 tgtgatcaat aggcgcagac atccgccttc atgagttcgc gcaggagcac ggtagcgtag
      361 caccccttgc ccaggaagaa cgtggcgacg tacccgtcct ccccgacctc cagccggagg
      421 tcccggtact cgcacaccag ctcccggcgg gagcccttgg aggagcagtg gggcacctgc
      481 ggcacgagga agtccctggg cgaaagcccc tcgcgctcga tcgccctccg ctcgatctcc
      541 cccatctccc cctccgccag ctccgagccg tagccgaaga gggtcgcgga gacgaaggcc
      601 ctgcgctccc tcacctgctc ttcaacgagg tccaggttgg ccgttgtcac aggcacgggc
      661 ttgtcgtggt ccgggttgcc gtccctgtcc gccggaagga cgacgtcgcc gacgatcggg
      721 gcgttcaacg gaatgccccg gcgcatccgc tccgagagga tgacgttgaa caggtaggac
      781 tgataggcgt gcacgaacat catctgcagg ttcggcggga ggacgcggat ggccccggcg
      841 tagtcctccg gattgcgctc caggtatccg atgaccatcc gttcgaaggt gagcgtccgg
      901 gggaaggtcc gcagcgccgc cgagtagtcg cggtcccgct ccagggcctc ccgcgcctcc
      961 cgggacggcc cctcctcatc ctcgctcggg ttgcctatgt acagcagcac cgcccgctcc
     1021 atgtccccct tcactatcgc ccttccgacc agatgcgtga ccgggcgcac cgccccgaag
     1081 cgctgcacgc cgaagaagtt ggggaagccg cccagctccc gcagggcctc ctgagtccct
     1141 tccagggagg accggagctc gcttcccctc agctcggtgt ccctgacata gatcttgaag
     1201 gtgttcccga ccaggtcgcc gagggccatc ccccgcctgg ccatgtatcc cccctcgacc
     1261 ctcacctgcg ggattcggag cgccatcacc cgctccaccg gggcctcgaa ggacatcagc
     1321 tgggtggtca cggcccgctt gtccttggtg ccggcgaacc cgatcccgtt gcgggagatg
     1381 cccagggcct ttgccatgtg ccgcacgagg cggttggtct cccagttcgt cgcggtcacc
     1441 cgggccacgc agtacctgcc gtccgttttc tccggcgggt acttggatac ctcctcgacc
     1501 acgaagtcct cggggcacgc cttgagcttg ccgccggtgc ccggggtgtc ggtgtagaag
     1561 acctccagcc cgatctcccg ctcgcgctgc gcgctgggcc gcaccatgaa aattacttca
     1621 ggtccttcag caaggcctcc ctggcctcct tgaactcgtt ggccagggcc ttcgccgtct
     1681 tcttcaaggc cgactggggc ttgcccgtcc ggacccggac gaaaagggtg gggtcctcca
     1741 tctggctgtg cccgatggtg tacttgacct ccgccacgtc cttgtcctcg agcagcccct
     1801 gcaccatggg ggtgatcagc gtcatgtccg cgcccttgat gcggaccttg atggagtcct
     1861 tttccttctc taagagctcg atctccatgg agtggaccaa tatgggggcg ggctataaac
     1921 cttttgacat cactccaccc gcttgagctg ggcctggtac atggccagta tctcctggct
     1981 cgtggtggcg tcccgccctt ccttgtcctc ccggaagcgc ccggtgaagc gggggaagcg
     2041 gatggccagg cccgcgtcgg gcctgatcgc cccgaagcag caggtgtgga tgggcgagag
     2101 ggtgatctcc gccccccgca cctccagcac cagccggggc tggaaccaga agtccgcctc
     2161 catcttcgag tccaccaggt ggtgcctcgc cgccagccgg tcgttcgcga gcatctcggg
     2221 cagtgccgcc agggtggcgt ccatgccggt ggtgttgttg tcgtcgagca gcaggcgcac
     2281 gcgtacgccg cgcccggcgg cctggcccag ggcctgcatg agcagggtgc cggtgaggtc
     2341 gccgtgccag atgtagtact gcacatccag actgcgctcg g
//
LOCUS       DAOSYN010000208         2359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_34667_length_2359_cov_3.128472, whole genome shotgun sequence.
ACCESSION   DAOSYN010000208 DAOSYN010000000
VERSION     DAOSYN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34667_length_2359_cov_3.128472"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..935)
                     /locus_tag="PLJ11_07025"
     CDS             complement(117..935)
                     /locus_tag="PLJ11_07025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO04453.1"
                     /translation="MSRREEASSGRSDTSYEEPDLYEVLGKYYDVWYEDQVEDTQFYL
                     RLAEQVGGPVLECMCGTGRTLVPFAEKGYEIWGFDRSHSMLDRLTAKMELYDEEVQAR
                     VSVHHADMRTFRCPRRFKLIVVPFNAFLHLLEREGQEAALRNVAEHLEEGGRFVMSVF
                     NPRLDRPEELVRHRGTRTMLNGEIISKFEAQTFDRQRRRTTVHYFLDISRQDREMRRV
                     TACFTIRYLAHQEVLGLMAASGLEVEATYGDWHGGPFTEESDIMVFVAKRAHAP"
     gene            complement(886..1368)
                     /locus_tag="PLJ11_07030"
     CDS             complement(886..1368)
                     /locus_tag="PLJ11_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOO04454.1"
                     /translation="MRQYNVPVEKPADVNVIIGQSHFIKTAEDLYEAIVTSVPQARFG
                     VAFCEASGERLVRVEGNDPELRECAQRNALKVGAGHTFFIVMRDAYPLNVLPRIRQVP
                     EVCCIFCATANELELVVAESALGRGVVGVIDGQMPVGVEQDADVEKRRGFLRKIGYKL
                     "
     gene            1454..2245
                     /locus_tag="PLJ11_07035"
     CDS             1454..2245
                     /locus_tag="PLJ11_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOO04455.1"
                     /translation="MLRTGPAGYPEGSRTPADAVRKVSEAGLDALEVQFVRDVRFNPE
                     RAEEARREAEARKVALSVHAPYYINLNSASQATVGKSEAWALRAMRGAEAFGAWIVVV
                     HAAMYGDDREGATRKVIGALRRVRRAAEEEGLRAAIGLETMGRRAAWGTLAEIRTVME
                     EVEGVLPVVDFAHLQARGPPLDRERLDTLLTDLADVPRLHCHLSGIEGGDAGERRHLR
                     LGEGLDHRMVLDALRRSGREATVICESPAPMEDARAIRRYLDGGG"
BASE COUNT          405 a          793 c          777 g          384 t
ORIGIN      
        1 ccaccctcgg gccgtcctgc tccagcacga ccatcctccc gcccactacc cccttccata
       61 ggcccccctc cttccgccag cggaacacct gcccgcagcc gagggtgtgg tccagatcaa
      121 ggagcatggg cccgcttggc cacgaacacc attatgtcgc tctcctcggt gaaggggcct
      181 ccgtgccagt ccccgtacgt cgcctctacc tccaggccgg aggcggccat cagccccagc
      241 acctcctggt gcgccaggta cctgatggtg aagcaggctg tgacgcgccg catctcccgg
      301 tcctggcggg agatgtcgag gaagtagtgc accgtggtgc gccgccgctg cctgtcgaag
      361 gtctgggcct cgaacttgga gatgatctcc ccgttgagca tggtccttgt cccgcgatgg
      421 cgcaccagct cctccgggcg gtccaggcgg gggttgaaga cgctcatgac gaagcgccct
      481 ccctcctcca ggtgctcggc gacgttgcgc agcgcggcct cctgcccctc cctctccagg
      541 aggtgcagga acgcgttgaa cgggacgact atcagcttga acctgcgggg gcaacggaag
      601 gtgcgcatgt ccgcatgatg gacgctcacc cgcgcctgga cctcctcatc gtacagctcc
      661 atcttggcgg tgaggcggtc cagcatggaa tggctgcggt cgaagcccca gatctcatac
      721 cccttctccg cgaacgggac caatgtgcgg ccggtgccgc acatgcactc caggacaggc
      781 ccgccgacct gctccgccag gcgcaggtag aactgggtgt cctcgacctg gtcctcatac
      841 cacacgtcgt agtacttgcc caggacctcg tagaggtccg gttcctcata gcttgtatcc
      901 gatcttccgg aggaagcctc ttctcttctc gacatctgca tcctgctcaa cccccactgg
      961 catctggcca tcgatcacac ccacgacccc acgccccaag gcgctctccg ccaccaccag
     1021 ctcaagctcg ttggccgtgg cgcagaaaat gcagcacacc tccgggacct gcctgatcct
     1081 cgggagcaca ttgagcggat aggcgtcccg catgacgatg aagaacgtat gccccgcccc
     1141 caccttcagg gcgttgcgct gcgcgcactc cctgagctcg gggtcgttgc cctccacccg
     1201 cacgagccgc tccccggacg cctcgcagaa ggccaccccg aacctggcct ggggaacgga
     1261 ggtcacgatc gcctcgtaca ggtcctcggc ggtcttgatg aaatgggact ggccgatgat
     1321 cacgttcacg tccgcgggct tctccaccgg cacgttgtac tgcctcattt ccctcccagt
     1381 ccaccaatcc tcggccggat atatacattg tacgccccgg cagctcctta aggcgggaat
     1441 aggaatatac gtcatgctcc gcaccggtcc ggccggctat cccgaaggct ccaggacgcc
     1501 cgccgacgcc gtgcggaagg tgagcgaggc tggactggac gcgctggagg tccagttcgt
     1561 gcgcgacgtg cggttcaatc ccgagagggc ggaggaggcc cggcgcgagg cggaggcgcg
     1621 aaaggtggcg ttgagcgtgc acgcccctta ctacatcaat ctgaactccg cgtcccaggc
     1681 caccgtgggg aagagcgagg cgtgggcgct ccgggcgatg cgcggggcgg aggcgttcgg
     1741 ggcctggata gtggtggtgc acgccgccat gtacggcgac gacagggagg gggctacccg
     1801 gaaggtcatc ggcgcgctga ggcgggtccg gagggcggcc gaggaggagg ggctgcgcgc
     1861 cgccataggc ctggagacca tgggccggag ggcggcctgg ggcacgctcg ccgagataag
     1921 gacggtcatg gaggaggtgg agggtgtcct cccggtggtc gatttcgccc acctgcaggc
     1981 gcgaggcccg ccgctggacc gggagcggtt ggacaccctc ctgacggacc tcgcggacgt
     2041 cccccggctc cattgccacc tcagcggcat cgagggcggc gatgccgggg agcggaggca
     2101 tctgcgcctc ggggagggtc tggaccaccg aatggtgctg gacgccctcc gccggagcgg
     2161 gagggaggcc acagtcatct gcgagtcccc cgcgccgatg gaggacgcca gggcgatcag
     2221 gcgctatctg gacggcgggg gctagtacgc gtactgaaaa gtacgcaggt tgaaataatg
     2281 tcaaggcaat aatggcccaa ggcggatggt catatgccag gtgcaaaaag ggaggccgag
     2341 ggggaagagg tcttcgagt
//
LOCUS       DAOSYN010000209         2357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_34702_length_2357_cov_5.420504, whole genome shotgun sequence.
ACCESSION   DAOSYN010000209 DAOSYN010000000
VERSION     DAOSYN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34702_length_2357_cov_5.420504"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLJ11_07040"
     CDS             <1..284
                     /locus_tag="PLJ11_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase subunit GuaA"
                     /protein_id="HOO04456.1"
                     /translation="AAARNGSMVRPWQYFAVLLPVQSVGVQGDRRAYGRTIAIRAVES
                     VDAMTAAFSRVPYDVLERISLRITNEMKANVNRVVYDITNKPPATIEWE"
     gene            complement(285..998)
                     /locus_tag="PLJ11_07045"
     CDS             complement(285..998)
                     /locus_tag="PLJ11_07045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO04457.1"
                     /translation="MKAVLFDLGHTLIDYYHDWSGPEERIVSRLHRLAMEAGGRMQEP
                     EFRRSVLEILRQNRDRKAQDMVEIPLERVLESIFGSAGCDWDESLVQDGLELFYEALR
                     EDRSLVPGTVEMLERVREKGYSIGLISDVAWGLPSEYPLRDIRHFGLDRYFDDMIFSS
                     DVGLRKPNPRMFKMAMLNLGVGRDEAMYVGNSLQADIRGARAVGIRAVLKRSGYFQPD
                     DSIVPDHTVDDWKELDGLL"
     gene            1095..1670
                     /locus_tag="PLJ11_07050"
     CDS             1095..1670
                     /locus_tag="PLJ11_07050"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HOO04458.1"
                     /translation="MRVYVVDNGGQWTHREWRVLRDLGVEARIVPNTTPFDHLGQVDA
                     LVLSGGAPDVASEAARMGNNGEYLDRADCPILGICAGMQFMAAHFGGRTGPAARAEFG
                     RTRLYVDEEDQLFVGIPREFDVWESHNDEVKDVPEGFQALAHSETCAVEAMRSLRRPL
                     YAVQFHPEVENTAFGTDIFRNFLRAVEEWRR"
     gene            1658..1876
                     /locus_tag="PLJ11_07055"
     CDS             1658..1876
                     /locus_tag="PLJ11_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04459.1"
                     /translation="MAPLREELRPALAAALEKRRRDDTVEMAVGREMRRMGLGYQDYL
                     EAMEAVRETARRRGLPPWKAVEAVTEGR"
     gene            1975..>2357
                     /locus_tag="PLJ11_07060"
     CDS             1975..>2357
                     /locus_tag="PLJ11_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04460.1"
                     /translation="MTTLNEVLKEIMEARGFLVDQVGPYVRGRREDIALYFYPYPRHS
                     PAAMEEIARTAKGLQGKVVLAALVPLAESSLANLPSGVVVWDREAVEHEVGRTRIERL
                     VGEEDHGLIDDLYADDVPVPPSETP"
BASE COUNT          406 a          783 c          787 g          381 t
ORIGIN      
        1 aggccgccgc aaggaacggt tccatggtcc ggccatggca gtacttcgcc gtgctcctcc
       61 ccgtgcagtc ggtgggggtg cagggggacc ggcgcgccta tggcaggacg atcgccatcc
      121 gggccgtgga atcggtggac gccatgaccg cggccttctc ccgggtcccc tacgacgtgc
      181 tggagcgcat ctccctccgc ataaccaacg agatgaaggc caacgtgaac cgcgtggtct
      241 acgacatcac caacaagccg ccggcgacga tagagtggga gtgatcacag cagcccgtcg
      301 agctccttcc aatcgtccac ggtgtggtcg gggacgatgg agtcgtccgg ctggaagtag
      361 ccgctccgct tgagcacagc cctgatcccc actgccctcg cccccctgat gtccgcctgc
      421 agggagttgc cgacgtacat tgcctcgtcc cttccgacgc cgaggttgag catggccatc
      481 ttgaacatgc gcgggttcgg cttgcgaagc ccgacatcgc tggagaagat catatcgtcg
      541 aagtacctgt ccagaccgaa atgccggatg tcgcggagcg ggtattcgga cgggagcccc
      601 caggctacgt ccgagatcag gccgatggaa tatccctttt ccctgaccct ctcgagcatc
      661 tccacggtcc cgggcacgag ggaccggtcc tccctcagcg cctcgtagaa gagctccaga
      721 ccgtcctgca cgaggctttc gtcccaatcg caccccgcgg agccgaatat gctctccaag
      781 accctttcca gagggatctc caccatgtcc tgggccttgc ggtcccggtt ctgccgcagg
      841 atctcgagga ccgagcgccg gaactccggc tcctgcatgc gcccccccgc ctccatggcc
      901 agcctgtgga ggcggcttac tatgcgctcc tccggccccg accagtcgtg gtagtagtcg
      961 atcagggtgt gccccaggtc gaatagcacg gccttcatgg tagtggataa cgagcaggcc
     1021 ggaaataatc gtttcccggg cccgttccgg ccccgaccca tcgttttata ccgcgcaccg
     1081 acgattcggc cccgatgcgg gtctacgtcg tggacaacgg ggggcaatgg acgcaccgcg
     1141 aatggcgcgt cctgagggac ctcggggtgg aggcgaggat cgtcccgaac accaccccct
     1201 tcgaccacct gggccaggtg gacgcgctgg tcctctcagg gggcgccccg gatgtggcct
     1261 cagaggccgc gcggatgggc aacaacgggg aatacctgga cagggccgat tgccccatcc
     1321 tcggcatctg cgccggcatg cagttcatgg ccgcccactt cgggggacgg accggtcccg
     1381 ccgcgcgcgc cgagttcggg cgcacccgac tgtacgtgga cgaggaggac cagctgttcg
     1441 tcggcatccc aagggagttc gacgtctggg agtcccacaa cgacgaggtg aaggacgtcc
     1501 cggaggggtt ccaggcactg gcgcactctg agacatgcgc ggtggaggcg atgcgctccc
     1561 tccgccgccc gctgtacgcc gtgcagttcc atcccgaggt ggagaacacc gccttcggaa
     1621 cggacatatt ccgtaacttc ctgagggcgg tggaggaatg gcgccgctga gggaggaact
     1681 ccgaccggcg ctggcggccg cgctggaaaa gcgccgtcgg gatgacacgg tggagatggc
     1741 ggtcggccgc gagatgcgcc gcatgggcct ggggtaccag gactacctcg aggcgatgga
     1801 ggcggtccgg gagacggccc gaaggagggg gctgcccccg tggaaggccg tcgaggccgt
     1861 cacggagggg cggtagcgcc cggtatcata cgccgccctc ggtggtcccc gtccccaacc
     1921 cttcggcgag gccgataagc tattaatccg ctcggcgcga tccctgtccc gagaatgacc
     1981 acgctcaacg aggtgctcaa ggagatcatg gaggccaggg gcttccttgt ggaccaggtc
     2041 ggcccctacg tcagggggcg ccgggaggac atagcgctct atttctaccc ctaccccagg
     2101 cactcccctg cggccatgga ggagatcgcc aggaccgcca agggcctcca gggcaaggtc
     2161 gtgctggccg cgctcgtgcc attggcggaa tcctccttgg cgaacctccc gtcaggcgtg
     2221 gtggtctggg accgggaggc ggtggagcac gaggtcggac gcacccgcat cgagcgactg
     2281 gtcggggagg aggaccacgg actgatcgac gacctctatg cggacgacgt gccggtccct
     2341 ccctcggaga ccccgga
//
LOCUS       DAOSYN010000210         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_34839_length_2350_cov_3.096296, whole genome shotgun sequence.
ACCESSION   DAOSYN010000210 DAOSYN010000000
VERSION     DAOSYN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34839_length_2350_cov_3.096296"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLJ11_07065"
     CDS             complement(<1..465)
                     /locus_tag="PLJ11_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04461.1"
                     /translation="MHNLEEIARTIQGSLEEKDEVREIAIKSSRALIRMASGVVHGMH
                     KGLDCAPELAEAIDEAHRLKSVLSEHVDIWHSGIVQDALVEVAEAALLNAIVKGQELP
                     DPYRLSMSGTAYLMGMADAIGELRRFALEALRRGDIPSAERYLDIMEELFLVI"
     gene            complement(500..1822)
                     /gene="purD"
                     /locus_tag="PLJ11_07070"
     CDS             complement(500..1822)
                     /gene="purD"
                     /locus_tag="PLJ11_07070"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOO04462.1"
                     /translation="MRTKVLVVGGGGREHAIVEALVRDGAEVYAAMRNRNPGIARAAK
                     GFRLLNETDVPEVAEFASSTGAEIAVIGPEAPLEAGLVDELLRRGIGCVGPSQAAARL
                     ETSKRFARRLLKKHGIPGNVAFGTFQDYEAAKLFVEGFPGELVVKPEGLTGGKGVKVE
                     GEHLRGKEEVLAYVREVLDNRIGGGGVVLEERLVGEEFTLQAFCDGRSVRGMPLVQDH
                     KRAYEGDVGPNTGGMGSYTDADHLLPFVTAEDRERAMDIMRRTVQAMRAEGTPYRGVL
                     YGQFMLTRDGPKVIEFNARFGDPEAMNVLTLLPNGLLDACLGVADGHLKGDVPFLRKA
                     TVCKYVVPAGYGTAPRAGMPVKVDEGALARTGAQLYYAAVEERDGTIYTTTSRALGVV
                     GMADSIEEAEAICEAGLANVAGDVLVRHDIGRAELVRKRVEHMRQVRG"
     gene            complement(1866..>2350)
                     /gene="hypB"
                     /locus_tag="PLJ11_07075"
     CDS             complement(1866..>2350)
                     /gene="hypB"
                     /locus_tag="PLJ11_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOO04463.1"
                     /translation="DAMAIMGLTNIAGDVTGQDDYDRFQAAGVRAYNLNTGKECHLDA
                     NLLDRALDGIDLLAIDFLFVENVGNLVCPADFPLGTDKRVVVISVTEGDDMVRKHPMI
                     FQGADVAVVNKMDLASYMGVDVERIKADYHRLSGGKELLPVSVRTGDGLEALADRLLG
                     "
BASE COUNT          340 a          903 c          705 g          402 t
ORIGIN      
        1 gatgaccagg aagagctcct ccatgatgtc caggtatctc tcggcggagg ggatgtcccc
       61 ccgccgcagg gcctccaatg caaaccgccg cagctcgccg atggcgtccg ccatgcccat
      121 aaggtaagcg gtgccggaca tgctcaggcg gtaggggtcc ggcagctcct gccccttcac
      181 tatggcgttc aggagggccg cctccgccac ctccacgagg gcgtcctgca ctatcccgga
      241 gtgccagatg tccacgtgct ccgagagcac gctcttgagc cggtgcgcct cgtctatggc
      301 ctcggcgagc tcgggagcgc agtccaatcc cttgtgcatg ccgtggacca ccccgctggc
      361 catgcgaatc agcgcgcggg aggacttgat ggcgatctcc cgcacctcgt ccttctcctc
      421 cagggacccc tggatcgtcc gcgcgatctc ctccaggttg tgcatggaag ggaatcgacc
      481 ggggatatga aaagcttgct cagccccgga cctggcgcat gtgctccacc cgcttccgca
      541 ccagctccgc cctgccgatg tcatgccgga cgaggacgtc ccccgcgacg ttcgccagcc
      601 ccgcctcgca tatggcctcg gcctcctcta tgctgtccgc catgcccacc acccccaggg
      661 cccgggaggt ggtggtgtag atcgtgccgt cccgctcctc caccgccgca tagtacagtt
      721 gggcgccggt cctcgcgagc gccccctcgt cgaccttcac cggcatcccg gccctcggcg
      781 ccgtgccgta gccggccggc acgacgtact tgcacaccgt ggccttcctc aggaagggca
      841 cgtccccttt caaatggccg tccgccacgc ccaggcacgc gtcgaggagg ccgttcggca
      901 ggagggtaag gacgttcatc gcctccgggt ccccgaaccg ggcgttgaac tcgatgacct
      961 tcgggccgtc cctcgtcagc atgaactgcc cgtacagcac cccgcggtac ggggtcccct
     1021 ccgcccgcat ggcctgcacc gtcctccgca tgatgtccat cgcccgctcc cggtcctccg
     1081 cggtcacgaa ggggaggagg tggtccgcgt cggtgtagga gcccatcccc ccggtgttcg
     1141 gccccacgtc cccctcgtac gcccgcttgt ggtcctgcac caggggcatg ccccggacgc
     1201 tccgcccgtc gcagaaggcc tgcagcgtga actcctcccc cacgaggcgc tcctcgagca
     1261 ccactcctcc cccgcctatc ctgttgtcca ggacctccct gacgtaggcg agcacctcct
     1321 ccttcccccg gaggtgctcc ccctcgacct tgaccccctt cccgccggtc agcccctccg
     1381 gcttcacgac cagctcgccc gggaacccct ccacgaacag cttggcggcc tcgtagtcct
     1441 ggaacgtgcc gaaggccacg ttgcccggga tgccgtgctt cttcaggagc cttcgggcga
     1501 agcgcttgga cgtctcgagg cgcgccgcgg cctgggaggg cccgacgcat ccgatgcccc
     1561 gcctcaggag ctcgtctacc aggcccgcct ccagcggggc ctccgggccg atgacggcga
     1621 tctccgcccc cgtcgaggag gcgaactcgg ccacctcggg cacgtccgtc tcattgagca
     1681 gccggaaccc cttggccgcc cgggcgatgc ccgggttgcg gttcctcatc gccgcgtaga
     1741 cctccgcccc gtccctgacg agcgcctcca cgatggcgtg ctccctcccc cctccgccga
     1801 ccaccagcac cttggtcctc atgcggggcg gggatgggca gggccgataa taaataaatg
     1861 ggcgctcagc ccagcaggcg gtccgccagg gcctctagcc cgtccccagt gcgcacgctg
     1921 accggcagga gctccttccc cccggagagc cggtggtagt cggccttgat ccgctccacg
     1981 tcgacgccca tgtacgacgc caggtccatc ttgtttacga cagccacgtc cgccccctgg
     2041 aagatcatgg gatgcttccg caccatgtcg tccccctcgg tgaccgagat gacgaccacg
     2101 cgcttgtccg tgcccagggg gaagtccgcc gggcagacga ggttccccac gttctcaacg
     2161 aagaggaagt cgatggccag caggtcgatc ccgtccaagg cgcggtctag caggttcgcg
     2221 tcaagatggc attccttgcc ggtgttgagg ttgtacgccc tcaccccggc ggcctggaac
     2281 cggtcgtagt cgtcctgccc ggtgacgtcc ccggcgatat ttgtcaggcc catgatggcc
     2341 atggcgtcgt
//
LOCUS       DAOSYN010000211         2343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_34950_length_2343_cov_3.851399, whole genome shotgun sequence.
ACCESSION   DAOSYN010000211 DAOSYN010000000
VERSION     DAOSYN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34950_length_2343_cov_3.851399"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLJ11_07080"
     CDS             <1..436
                     /locus_tag="PLJ11_07080"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOO04464.1"
                     /translation="GLPMGLGEPFFDTVEGELAKMMFAVPGVKGIEFGAGFAAAGMRG
                     SQHNDPFVLVDGKVRTAKNDAGGILGGITNGMPVVLRVAIKPTASIARGQRSLDLETG
                     EQTDLRIEGRHDPCIAPRAVPVVEAAAALVLADLSVRGGMLD"
     gene            429..1505
                     /locus_tag="PLJ11_07085"
     CDS             429..1505
                     /locus_tag="PLJ11_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOO04465.1"
                     /translation="MTDSVEGIRWKIEEIDNQILDLIKRRMEIALSMGQQKVEKALPV
                     RDLRVEEQVRTRYLARAKEVGISEAAAIELATLLVRESVEAQARLPRPMRPRKVLVVG
                     GGGAMGQWLCRFFASRGHEVTVSDAQPGGGFPNVGLEEGVRGADVIAVSTHISATEEA
                     LRKVLRLRPKGLVFDIASVKAPFIDVLREGAAQGLLVCSIHPMFGPSASSMFARNLIV
                     CNCGSDAAVGRFMPLLNGTGANVVEMPVEDHDRLIAIVLGMSHALNLAFFGALRASGF
                     SYEELSRVASTTFERQMSISKSVAMESPNLYYEIQHLNPHNREILDRLIDALREVQQA
                     GCDEDREHMIRLMQEGRKYFEVKR"
     gene            1502..>2343
                     /gene="asd"
                     /locus_tag="PLJ11_07090"
     CDS             1502..>2343
                     /gene="asd"
                     /locus_tag="PLJ11_07090"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOO04466.1"
                     /translation="MRKVQVAVLGATGMIGQRFVQLLEGHPYFEIGGLYASERSEGKT
                     LGEVLRVKGVRFQDETLAMRIEALEPKAIARRCRLAFSGLPTEVAKDAEAALADAGVA
                     VFSNAASHRMRADVPLLIPEVNPDHLELARSQATFPQGGFIVTNANCSTTGLAVPLKA
                     VHAAFGLRACYVSTYQAVSGAGYPGVPSLDILGNVVPFIRNEEEKMEEEIHKMLGTYK
                     GGRVEPAAFDMVASCARVPVLDGHLESVVLRLGREAAAAEVVEALETFRPEPQALGLP
                     TAPLR"
BASE COUNT          380 a          734 c          883 g          346 t
ORIGIN      
        1 cgggctcccg atggggctgg gggagccctt cttcgacacg gtcgagggag agctggcgaa
       61 gatgatgttc gccgtgccgg gggtgaaggg catcgagttc ggggcgggct tcgccgccgc
      121 tgggatgcgg ggctcgcagc acaacgaccc cttcgtgctg gtggacggga aggtgcggac
      181 ggcgaagaac gacgccgggg ggatcctcgg ggggatcacc aacggcatgc ccgtggtcct
      241 ccgggtggcg atcaagccga cggcgtccat cgcccggggg cagcgctccc tggacctgga
      301 gacgggcgag cagaccgatc tccggataga ggggcggcat gacccgtgca tcgcccccag
      361 ggcggtgccg gtcgtggagg cggccgccgc gctagtgctg gcggacctga gcgtgcgagg
      421 tgggatgctt gactgattcc gtggagggca ttcgctggaa gatagaggag atcgacaacc
      481 agatcctgga cctgatcaag aggcgcatgg aaatcgccct gagcatgggg cagcagaagg
      541 tggagaaggc cctgccggtg cgggacctgc gggtggagga gcaggtgcgg acgaggtacc
      601 tcgcgcgggc gaaggaggtg gggatcagcg aggcggccgc gatcgagctg gcgaccctgc
      661 tcgtcaggga gtcggtcgag gcccaggccc ggctgccccg cccgatgcgg ccgcgcaagg
      721 tcctggtggt cggcggcggg ggggcgatgg ggcagtggct gtgccgcttc ttcgcctccc
      781 gcgggcacga ggtgacggtg agcgacgccc agcccggggg aggattcccg aacgtggggc
      841 tggaggaggg ggtgcggggg gcggacgtca tcgcggtgtc cacgcacatc tccgccaccg
      901 aggaggcgct gcggaaggtg ctccggctga ggccgaaggg gctggtgttc gacatcgcct
      961 cggtcaaggc ccctttcatc gacgtcctga gggagggcgc ggcccagggg ctgctggtct
     1021 gctccatcca ccccatgttc ggcccgagcg cctcctccat gttcgcccgg aacctcatcg
     1081 tgtgcaactg cggctccgac gcggccgtcg gccggttcat gcccctgctg aacgggacgg
     1141 gggcgaacgt cgtcgagatg ccggtcgagg accacgaccg gctcatcgcc atcgtcctcg
     1201 ggatgagcca tgcgctcaac ctggcgttct tcggggcgct gcgggcgagc gggttcagct
     1261 acgaggagct gagcagggtg gcctccacca cgttcgagcg gcagatgagc atcagcaaga
     1321 gcgtggccat ggagagccca aacctttact atgagatcca gcatctgaac ccgcacaacc
     1381 gggagatcct ggataggctg atcgatgccc tgagggaggt ccagcaggcg gggtgcgacg
     1441 aggaccggga gcatatgatc aggctcatgc aggagggaag gaagtacttc gaggtgaaac
     1501 gatgaggaag gtgcaggtgg cggtcctagg cgccacagga atgatcggcc agaggttcgt
     1561 gcagctgctc gagggccatc cctatttcga gatcggcggc ctatacgcct ccgagcggtc
     1621 ggaggggaag acgctcgggg aggtcctgag ggtgaagggc gtccgcttcc aggacgagac
     1681 cctcgccatg agaatagagg cgctggagcc caaggccatc gcccggcggt gccggttggc
     1741 gttctcgggc ctccccaccg aggtggctaa ggacgccgag gccgcgctcg ccgatgccgg
     1801 cgtagcggtg ttctccaacg ccgcctccca ccgcatgcgg gcggacgtcc cgctgctcat
     1861 ccccgaggtc aacccggacc acctggagct ggcgcgctcc caggcgacct tcccccaggg
     1921 cgggttcatc gtcaccaacg cgaactgctc gaccaccggc ctcgccgttc cgctcaaggc
     1981 cgtccacgcc gcgttcgggc tcagggcctg ctacgtctcc acgtaccagg ccgtctccgg
     2041 cgccggctac ccgggcgtcc cgtccctgga catcctcggg aacgtggtcc ccttcatccg
     2101 gaacgaggag gagaagatgg aggaggagat ccacaagatg ctcggcacat ataagggcgg
     2161 cagggtcgag cccgccgcct tcgacatggt ggccagctgc gcccgcgtcc cggtgctgga
     2221 cgggcacctg gaatcggtcg tcctgaggct ggggagggag gccgccgccg ccgaggtcgt
     2281 cgaggccctg gagacgttca ggcccgagcc ccaggcgctc ggcctgccca ccgctcccct
     2341 ccg
//
LOCUS       DAOSYN010000212         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_35123_length_2334_cov_3.142168, whole genome shotgun sequence.
ACCESSION   DAOSYN010000212 DAOSYN010000000
VERSION     DAOSYN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35123_length_2334_cov_3.142168"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="PLJ11_07095"
     CDS             complement(<1..2281)
                     /locus_tag="PLJ11_07095"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO04467.1"
                     /translation="MTILLLLTFLVGVDLPAGEVSAIQYTITVLDPNGGESLVGATDF
                     EIMVYTSTVNGVIVLSYSTNGGETYPNGIDVIPNVGGTHRYSWRVPNNVNSTHVKVRA
                     EWRSQEGDPSLLWASDESNGNLTITPGVTLEFLEVPDVMSYGRYSLVRWGLWDGLQIV
                     GGLSLQARYRTDTTWGDWFDLGGIFANIAPSQGGIWYMPDYYESAYGQLRITAYTEIP
                     GGTLITDAESEEFEISSPWVELVYPTGGQTLVGGSVCTITWASANDEGGIISGAFLEY
                     SIDGGVNWVFIDGGPNDWSYDWTVPSGMSSDHVRVRVGLYHTEWAILTTDESDSDLRI
                     IESADVPSVTLITPNPHIPGNIVYRHGEVTWIRWSATCFEGGISDFRILLSTDNGSTY
                     SPLMNASAASRAKAWTVPALDTMEAKIKIEMRLTDTRVVSAESSNPFYIFTETVWNRP
                     PVAIAPEDLNVLEGQSVTLDGSASYDPDGDALTYQWEVVDSLGFPVTLAGANTAVATF
                     VPNIRDITITMTFRLTVSDGQEINVEHYTDHMKVTSVRVAPAGPSISGFTPSNGFEGT
                     KVCINGTNLMGAQVRINGVLTATVPTAPTPSDPNPDRMYNFTLVPGIPIAPGPITVTT
                     RAGTATSGEDLDVHPRPWYCLDHGFNMYNTNKYFLSYPWAPWNDGDYRRTFGNDVYIN
                     IWVCVGIPYWTFWDGWECAGYLIEEPIAPDPFAALYYGAAYCYLARSGECFGFSSVSL
                     EFYHDLIDPNDLQPGAYDVD"
BASE COUNT          443 a          728 c          700 g          463 t
ORIGIN      
        1 cgtccacatc gtacgcgcca ggttgcaggt cattggggtc gatcagatcg tggtagaact
       61 ccaagctgac cgagctgaag ccgaagcact cccccgaccg agcgagatag caataggccg
      121 ctccataata aagggcggca aaggggtcgg gggcgatggg ctcctcgatc aggtaccccg
      181 cgcactccca cccgtcccag aacgtccaat aggggatccc gacgcagacc catatgttga
      241 tgtatacatc attgccgaag gtgcggcgat agtccccatc gttccaaggc gcccaggggt
      301 aggaaaggaa gtacttgttc gtgttgtaca tgttgaatcc gtggtccagg cagtaccacg
      361 gtctgggatg gacatcaagg tcctccccgg aggtcgccgt ccccgccctc gtcgtcacgg
      421 ttatgggacc gggggcgatc ggaatcccgg gcacgagggt gaagttgtac atccggtcgg
      481 gattggggtc ggagggcgtg ggggcggtgg gcaccgtggc cgtgagcacc ccgttgatgc
      541 gcacctgcgc ccccataagg ttcgtcccgt tgatgcacac cttggtgccc tcgaagccat
      601 tggagggggt gaagccactg atgctcggcc cggcgggggc gaccctcacg ctggtgacct
      661 tcatgtggtc agtgtaatgc tccacattga tctcctgccc atcgctcacc gtaagccgga
      721 acgtcatggt gatcgtgatg tcgcggatgt tagggacgaa cgtggccacc gccgtgttgg
      781 ccccggccaa ggtcaccggg aagcccaacg aatcgaccac ctcccattga taggtcaacg
      841 cgtccccgtc cgggtcgtag cttgccgagc cgtccagcgt cactgactgc ccctcgagca
      901 cgttcaggtc ttcaggggcg atcgcgacag gaggcctgtt ccataccgtt tcggtgaaga
      961 tgtagaaggg attggagctc tccgcactga ccaccctggt atcggtgagc ctcatctcga
     1021 tttttatctt cgcctccatg gtgtccagcg ccggaacagt ccaggccttg gctcgactgg
     1081 ccgccgatgc gttcatcagg ggggaatagg tgctgccgtt gtccgtggac agtaggatcc
     1141 taaaatcgga gatgcccccc tcgaagcagg tggcgctcca ccgtatccac gtgacctccc
     1201 cgtgccggta gacgatgttc cccggaatgt gcgggttcgg ggtgatcaag gtgaccgagg
     1261 ggacgtccgc gctctcaatt atcctgaggt cggaatcgct ctcgtccgtg gtcaagatgg
     1321 cccactcggt atgatatagg cctacgcgga cccgcacatg gtcgctgctc ataccggacg
     1381 gaaccgtcca atcgtagctc cagtcattgg gcccgccatc tatgaaaacc caattcacgc
     1441 ccccatcgat ggaatattcc aggaaggcgc ctgagatgat cccgccctcg tcgttggccg
     1501 aagcccacgt gatggtgcac acgctcccgc ccaccaacgt ctgtccgccc gttggataga
     1561 cgagctcgac ccagggcgag ctgatctcaa actcctcgct ctcggcatcg gtgatcaggg
     1621 tcccgccagg gatctcggtg taggccgtga tccgcagctg tccgtacgcg ctttcataat
     1681 aatcgggcat gtaccagata cccccttgcg aaggggcgat attggcaaag atgcctccaa
     1741 ggtcgaacca gtccccccag gtcgtatcgg tcctatagcg cgcctgcagg ctcagcccgc
     1801 ctacgatctg caggccgtcc cacagccccc atcgtaccag ggagtacctc ccgtagctca
     1861 tgacgtccgg cacttccagg aactctaggg tcaccccagg ggtgatggtg aggttgccat
     1921 tgctctcgtc cgatgcccag agaagggacg ggtccccctc ctggctgcgc cactccgccc
     1981 tcaccttgac atgagtggaa ttgacgttat tgggcacccg ccaggaatag cgatgggtgc
     2041 caccgacgtt gggaatgaca tcgatcccgt tgggatatgt ctccccgccg ttcgttgagt
     2101 aggagaggac tatcaccccg ttgaccgtag aggtgtacac catgatctcg aagtccgtgg
     2161 cgccgaccag tgactcgccc ccgttcgggt ccaatacggt tattgtatac tggatcgcgc
     2221 tgacctcgcc cgcgggaagg tcgaccccca caaggaatgt caatagcaat aggatagtca
     2281 cggtcgtagc cccggccttc tggcctgggc tgcaacgctc ctgaccggtc gccg
//
LOCUS       DAOSYN010000213         2332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_35158_length_2332_cov_3.776460, whole genome shotgun sequence.
ACCESSION   DAOSYN010000213 DAOSYN010000000
VERSION     DAOSYN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35158_length_2332_cov_3.776460"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..2042)
                     /locus_tag="PLJ11_07100"
     CDS             complement(144..2042)
                     /locus_tag="PLJ11_07100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF018483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOO04468.1"
                     /translation="MELFPYPPRPHQADFVRTVERTMLANGHLVVESGTGTGKTVCAL
                     TGALQAALATGRKVLYLTRTNSQERQVILEVRRINERAPVFGVALQGRQGTCPLIRRD
                     KELSAGTPDELSRICQDKRRRVLEGRSGGCRFFKGLQDLDMEGFVDRCRQTLPTAEEL
                     IDQCDALNICPHEVMKELAKEARVVAAPYAYFFVPFIRNSLLDWMGCPMEDVLLIIDE
                     AHNLPDYTREIESFQLSRYMLEMVRKETEEFGDPDISKGVGLNDLCEGCSKLLEEALR
                     DYLIDEDGLVPPSFLEEGLMSAFTCTSRSLQAMAKLVMEQGEVIREARFAQGRLPRSY
                     THSLGAFLSYWFSLDEECYVKLVVGGENPCLKAYCIDPVVTTSALLGCGGSLHMSGTL
                     APLNEYRDSIGLPMDTALVSFPSPFPKENRRVFYVQDVSTKYEEMQSSPEMVEAMEDH
                     VVQVCNGVRRNTVVFFPSYALMDRFLADGVSRRISRKVHVEERGMAQLELMDVVTQFK
                     RAAPEGQVLFAVMGGRISEGIDFPEEELEVAVVVGIPYPRPTAKQKALMHFYELRFHR
                     GWEYTVKGPAIRKLLQAIGRLIRTERDVGAALVLDRRISQFGDRLDLVPTEHPLQDIQ
                     AFFRERGR"
     gene            complement(2110..>2332)
                     /locus_tag="PLJ11_07105"
     CDS             complement(2110..>2332)
                     /locus_tag="PLJ11_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04469.1"
                     /translation="EDRDAFYQLEDTGILTTEREETTLYDGREWRIHYWLFRKDRIYQ
                     LINSYGSDDSCDVDAYDCYADPSAWKRST"
BASE COUNT          397 a          775 c          735 g          425 t
ORIGIN      
        1 ggccgaggtc cacgggcgtc cctcccccga agatggtcgc cgcaggattg gggagcaggg
       61 aagggagaac gagccagagg cctgcaagga ccagcatggg gatgtccatg ggcgcgaatc
      121 ctccccgccg gttatgaacc tttctacctg cccctttcgc ggaagaacgc ctggatgtcc
      181 tgcagcggat gctcggtcgg caccaggtcc aaacggtccc cgaactggga gatgcggcgg
      241 tccagcacca aggccgcccc cacgtccctc tccgtgcgga tcagccggcc gatggcctgc
      301 agcagcttgc ggatggccgg ccccttcacc gtgtactccc accccctgtg gaacctgagc
      361 tcgtagaagt gcatcaatgc cttctgctta gccgtcggcc tggggtaggg gatgcccacg
      421 accaccgcca cctccagctc ctcctccggg aaatcgatgc cctcgctgat ccgtccgccc
      481 atgaccgcga acagcacctg cccctccggg gcggccctct tgaactgcgt tactacgtcc
      541 atcagctcca gctgggccat tcccctctcc tctacgtgca ccttgcggga gatgcgccgg
      601 gacaccccat cggcgaggaa gcggtccatc agcgcgtagg aggggaagaa gacgacggtg
      661 ttccgcctca ccccgttgca tacctggacg acgtggtcct ccatcgcctc caccatctcc
      721 ggggaggact gcatctcctc gtacttggtg gagacgtcct gcacgtagaa cacccggcgg
      781 ttctccttgg ggaaggggga agggaaggag accagggcgg tgtccatggg caggccgatc
      841 gagtcccggt actcgttcag gggcgccagc gtcccggaca tgtgcaggct gccgccgcag
      901 cccaggaggg ccgaggtggt caccaccggg tctatgcaat aggccttgag gcaggggttc
      961 tccccgccca ccacgagctt cacgtagcac tcctcgtcca aggagaacca gtaggagagg
     1021 aaggccccga gggaatgcgt gtacgacctt ggcagccgcc cctgggcgaa gcgggcctcc
     1081 ctgatcacct ccccctgctc catcaccagc ttggccatgg cctgaaggga gcgcgaggtg
     1141 caggtgaacg cgctcatcag cccctcctcc aggaaggagg ggggcacgag gccgtcctcg
     1201 tcgataaggt agtcccgcaa cgcctcctcc agcagcttcg agcacccttc gcacaggtcg
     1261 ttcaggccga cccccttgga gatgtcaggg tcgccgaact cctccgtctc cttccgtacc
     1321 atctccagca tgtagcggga tagctggaag ctctcgatct ccctcgtgta gtcgggcagg
     1381 ttgtgcgcct cgtctatgat gaggagcacg tcctccatcg ggcatcccat ccagtccagg
     1441 agggagttgc ggatgaaggg gacgaagaag taggcgtagg gagcggccac gaccctggcc
     1501 tccttcgcca gctccttcat cacctcgtgc gggcagatgt tgagcgcatc gcattggtcg
     1561 atgagctcct cggccgttgg cagggtctgg cggcacctgt cgacgaaccc ctccatgtcg
     1621 aggtcctgca gccctttgaa gaaccggcat ccccccgagc gcccctcgag cacccggcgg
     1681 cgcttgtctt ggcagatcct ggagagctca tccggggtcc cggcgctaag ctccttgtcc
     1741 ctccggataa gggggcaggt gccctggcgg ccttgcaggg ctacaccgaa taccggcgcc
     1801 ctctcgttga tcctcctgac ctcgaggatg acctgcctct cctgggagtt ggtgcgggtg
     1861 agatagagca ccttcctgcc cgtggcaagg gcggcctgca gggctcccgt aagggcgcat
     1921 accgtcttgc cggtgcccgt gccgctctcc accaccaaat ggccattggc cagcatggtg
     1981 cgctccacgg tgcgcacaaa gtcggcctga tggggacggg gaggataggg gaacagctcc
     2041 acgttcggtc agaatgccgt tgggataaat agtgttgccc tggggccgtt gaaagtgggc
     2101 ctagccggcc tacgtggacc ttttccacgc cgacgggtcg gcgtagcagt cataggcgtc
     2161 cacgtcgcag ctgtcgtcgc tgccgtagct gttgatcagt tgatatatac ggtccttgcg
     2221 gaagagccag taatggatgc gccattccct gccgtcgtac agggtggtct cctcgcgctc
     2281 ggtggtcagg atgcccgtgt cctcgagctg atagaacgcg tcccggtcct cc
//
LOCUS       DAOSYN010000214         2323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_35318_length_2323_cov_3.149030, whole genome shotgun sequence.
ACCESSION   DAOSYN010000214 DAOSYN010000000
VERSION     DAOSYN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35318_length_2323_cov_3.149030"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1214)
                     /locus_tag="PLJ11_07110"
     CDS             complement(84..1214)
                     /locus_tag="PLJ11_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO04470.1"
                     /translation="MSLIGAETFNWEDGNKRNNIMYRMPRNIKFNDNIVVREDEIAVF
                     YRDGKALAYLDRPDRYALTSLNAPIIGKVIEALSGARQQAEVIYLQKRAFDGKYGSKQ
                     PYQFRDAEFGMVNLRVFGEFRYKVGSPENFVNQFVGTFNFATSAEVEERIKEQMVMLI
                     YNAIGELKNRGMGVADLASQLMNIEQIVLTKAKDHFELYGVVVDKVSGMYISLPDEVQ
                     KAVDMRASMQILNTSYATYQAGQAMREAASNPAGGAAAAGVGVGAGFGAGYMMVDAMR
                     QGGTAPPAGAPAVAVPPVAGTVACPKCSTPNPQDAKFCRSCGSRLQNGTVKCGRCGSE
                     VPAGSKFCAECGQQLKGSKSCPKCGKESPVTAKFCPECGTGL"
     gene            complement(1219..1752)
                     /locus_tag="PLJ11_07115"
     CDS             complement(1219..1752)
                     /locus_tag="PLJ11_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04471.1"
                     /translation="MPDIRDRVEEDRGLLKKIQTVVPGFRGYRIREDLRDSDRMLRAE
                     LAKRLRAQRAQLEDARSALVRTDPMSKALEEIGGVVNAMKRVEAEMLHSEVGYSGVAA
                     DVRIRDDELNMLYEYDASMIESLGFIDQALVRVPEAIRAGNDGDLRQAVGTLRARVDG
                     LEDRFKRRMTAITGTGL"
     gene            complement(1805..2005)
                     /locus_tag="PLJ11_07120"
     CDS             complement(1805..2005)
                     /locus_tag="PLJ11_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04472.1"
                     /translation="MALNLLWLGTIWGAAVGIILADILYYFTDSLASFLLFIPCALVG
                     YLIAWRALRKTKTSEEHAEEKR"
BASE COUNT          418 a          776 c          632 g          497 t
ORIGIN      
        1 gagcatttca cgaacctatc gccggcatcg aactccaccg gggcggcgca cttggggcac
       61 ttcatgctgg ccattggagc acctcagagg ccggtgccgc actccgggca gaacttggcg
      121 gtgacggggg actcctttcc gcacttgggg cagctcttgc tgcccttgag ctgctgcccg
      181 cactcagcgc agaacttgga gcccgcgggc acctcactgc cgcaccttcc gcacttgacc
      241 gtgccattct gcagcctgct cccgcagctg cggcagaact tagcgtcctg ggggttcggc
      301 gtgctgcatt tcgggcatgc gaccgttccc gcgaccgggg ggaccgcaac ggccggcgcc
      361 cccgccgggg gggccgtccc gccctggcgc atcgcgtcca ccatcatgta ccccgccccg
      421 aagcccgcgc ccacgccgac cccggccgcg gccgccccgc cggcggggtt ggaggccgcc
      481 tcccgcatcg cctggcccgc ctggtaggtg gcgtagctgg tgttgagtat ctgcatggac
      541 gcccgcatgt ccaccgcctt ctgcacctcg tccggcaggg agatgtacat gcccgagacc
      601 ttgtccacca ccaccccgta cagctcgaag tggtccttgg ccttggtcag cacgatctgc
      661 tcgatgttca tcagctggct tgccaggtcc gccaccccca tcccccggtt cttgagctcc
      721 ccgatggcgt tgtagatgag catcaccatc tgctccttta tccgctcctc gacctccgcg
      781 gacgtggcga agttgaaggt gccgacgaac tgattgacga agttctccgg ggacccgacc
      841 ttgtacctga actccccgaa gacgcggagg ttgaccatcc cgaactcggc gtcccggaac
      901 tggtacggtt gcttgctgcc gtacttcccg tcgaacgccc tcttctgcag atagatgacc
      961 tcggcctgct gccgggcccc cgagagcgcc tcgatcacct tccctattat gggggcgttg
     1021 agggacgtga gggcgtaccg gtccggacgg tccaggtagg caagcgcctt cccgtcccga
     1081 tagaacacgg cgatctcgtc ctctctgacc acgatattgt cgttgaactt gatgttgcgg
     1141 ggcatgcggt acatgatgtt gttccgcttg ttcccgtcct cccaattgaa tgtctcagca
     1201 ccgataaggc tcatctgctc acagccccgt tcctgttatt gcggtcatcc ggcgtttgaa
     1261 gcggtcctcc agcccatcga ccctggccct cagtgtcccc accgcctgcc tcaggtcgcc
     1321 atcgttcccc gccctgatcg cctccgggac cctcaccagc gcctggtcga tgaatcccag
     1381 gctctcgatc atcgaggcat cgtactcata gagcatgtta agttcatcgt ccctgatccg
     1441 tacgtccgcg gcgaccccgg aatagcccac ctcggaatgg agcatctccg cttcgaccct
     1501 cttcatcgca ttgacgaccc cgccgatctc ctccaaggcc ttggacatcg ggtccgtcct
     1561 caccaatgcg ctccgggcat cctcaagctg ggccctctgc gccctcagcc tcttggccag
     1621 ctcggccctg agcatgcggt cggagtccct gaggtcctcc ctgatcctgt acccgcggaa
     1681 ccctgggacg accgtttgga tcttcttgag aagcccccgg tcctcctcga ccctgtccct
     1741 gatgtctggc aaaggaatcc ctctcctggc gtggatatcg cagggacgaa ttaaaaatgt
     1801 tggcctagcg tttctcctca gcatgctcct cggaggtctt cgttttacgg agggccctcc
     1861 aggcgatgag gtagccgacg agggcgcacg ggatgaacag caggaatgat gccagtgaat
     1921 ccgtgaaata gtacaggata tcggccagga tgatccccac cgccgccccc catatggttc
     1981 cgagccatag cagattgaga gccatttacc gcacctcgcg cttaggaacg attctaacga
     2041 taaggaattt actatcgggc agttcataga caaatgacta atattaacgg tgttataggt
     2101 tatatacacg ttcatttgtt tattcttatt atatagtata tagacattta tctattttta
     2161 atctaagaac attcaaataa ttgacattgg atgagcaggc ccgaacccta tgaggggagc
     2221 gtgatggcct tgaacagcat cgagaacagc agatcgacca cgggcaccaa gacccgggtg
     2281 cctgacgtca gcccattgaa cggcatgtgc cccatatgca tcg
//
LOCUS       DAOSYN010000215         2307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_35620_length_2307_cov_3.811723, whole genome shotgun sequence.
ACCESSION   DAOSYN010000215 DAOSYN010000000
VERSION     DAOSYN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35620_length_2307_cov_3.811723"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLJ11_07125"
     CDS             complement(<1..261)
                     /locus_tag="PLJ11_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04473.1"
                     /translation="MGKDMRPFFVMPGSALKDLREELQLLNGADSARTMERYGYRAGV
                     GLVRTLGLECADISEAKAIIEQVWTETGLSRMSIEMINETEIV"
     gene            complement(263..769)
                     /locus_tag="PLJ11_07130"
     CDS             complement(263..769)
                     /locus_tag="PLJ11_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HOO04474.1"
                     /translation="MAKRRFVKKVCLLGDGEVGKTSLIRRYVLDQFDDQYLQTFGAKV
                     SKKVLELQDVTLTLMIWDVLGQKTQKALHSTYYAGANGALLVGDMTRPETVEHLEHWA
                     RDLREVAGDIPIILLGNKCDLDMRLDLMGLERLASALGCRSMLTSAKTGQGVQEAFRA
                     LGEMTMEG"
     gene            920..1429
                     /locus_tag="PLJ11_07135"
     CDS             920..1429
                     /locus_tag="PLJ11_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO04475.1"
                     /translation="MTRSEKRFEDVTVGDVFDKVVLKPSQVQRGATIGTVIDQMLANP
                     LSRKVYVTEEDGRYAGTVSTETILRLMGYRLGIREYGGMSFIRFLRDTLKERADEIMV
                     KGRTVTRETRLPAAMRIMIEDHLNDLPVVDGANRLMGELVSLELFLVGKKVFEAKDPS
                     TFAIMADEA"
     gene            complement(1438..2199)
                     /locus_tag="PLJ11_07140"
     CDS             complement(1438..2199)
                     /locus_tag="PLJ11_07140"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOO04476.1"
                     /translation="MSEGEIFIHEYGEVDLKDGMAIIGFPSVGLVSSIASNFIVRTMR
                     LERRAAIISKHFPPFTIIHDGVPSPPVRIYAGHRDSEKKGERCEKLAVITCEFMPQPE
                     LIKELSDDILGWCKKKGIATILTLEGINMGGEGEAKVLGVGTTERTRGMLTSYGVKEL
                     REGMVMGISGLLLSEGERYQMDVICLLGSARTDVPDARGAANLLEVVGDMLPEIKLDP
                     EPLIKEAEQIENEMQKAMESVQQPKKQVEYSQLYG"
BASE COUNT          444 a          725 c          662 g          476 t
ORIGIN      
        1 cacgatctcc gtctcgttga tcatctcgat gctcatcctg ctgaggcctg tctcggtcca
       61 cacctgctct attatagcct tggcctcgct gatgtcggcg cactccaggc ccagggtgcg
      121 caccagcccc acgccggccc gatagccgta gcgctccatg gtgcgcgcag agtccgctcc
      181 gttcagaagc tgaagctcct cgcgcaggtc cttcagggcc gagccgggca tgacgaagaa
      241 ggggcgcatg tccttgccca tgtcaaccct ccatggtcat ttcccccagg gctcggaagg
      301 cctcctgaac cccctgtccg gtcttggcgc tcgtgagcat cgagcggcac ccgagggcgg
      361 atgcgagcct ctccaacccc atcaggtcca acctcatatc gaggtcgcac ttgttcccga
      421 gcaagatgat cgggatatcg ccagccacct cccgcaggtc cctcgcccaa tgttcgagat
      481 gctccacggt ctcgggcctg gtcatgtcgc ctacgaggag ggcgccgttc gcgccggcgt
      541 aataggtgga atgcagggcc ttctgggtct tctggcctag aacgtcccag atcatcaggg
      601 taagggtcac gtcctgaagc tccaggacct tcttgctgac cttggccccg aaggtctgca
      661 ggtactgatc gtcaaattgg tccagcacat accgtcggat gaggctggtc ttccccacct
      721 ccccatctcc aagcaggcag accttcttga cgaaccgacg cttagccatc ccgcattcag
      781 tatgcggcca gcgtctatat attcatttca cctcagccct tgcccccacc ctccccagaa
      841 cggggtttca gagccgttcc ttcccttgga acataaaagc gcccaaataa taatgtacct
      901 ccgttcagaa tcacttgcca tgacgcgaag cgagaagagg ttcgaggacg tcaccgtggg
      961 agacgtattt gacaaggtcg ttctgaagcc ctcccaggtg cagagagggg ctaccatcgg
     1021 aacggtgata gaccagatgc tcgcgaaccc gctttcccgc aaggtctacg tcaccgagga
     1081 ggacggaagg tacgccggca cggtgagcac ggagaccatc ctgaggctga tgggctaccg
     1141 attggggata cgggaatatg gcgggatgtc cttcatccgc ttcctgcggg acacattgaa
     1201 ggagagggcg gacgagatca tggtcaaggg aaggacggtg acccgggaga ccaggctccc
     1261 ggcggcgatg aggatcatga tcgaggacca tctgaacgac cttccagtgg tggacggcgc
     1321 caacaggctg atgggcgagc tggtcagcct ggagctgttc cttgtcggga agaaggtctt
     1381 cgaggccaag gacccctcca ccttcgccat catggccgac gaggcctgag gttagcgtta
     1441 gccgtagagt tgggagtact ccacctgctt cttgggttgc tgcaccgatt ccatggcctt
     1501 ctgcatctcg ttctcgatct gctcggcctc ctttatcaac ggctcggggt ccagctttat
     1561 ctccggcagc atgtcgccca ctacctccaa gaggttcgcc gctccccggg catcgggaac
     1621 gtccgtacgg gcggagccca ggaggcagat gacatccatc tgatacctct ccccttcgga
     1681 gagcagaaga ccggagatgc ccatgaccat gccctctctc agctccttta ctccgtagga
     1741 agtgagcatc cccctagtcc gctcggtggt gcccaccccc aggaccttgg cctccccctc
     1801 ccctcccatg tttatgccct ccagggtcag gatggtggcg atgcccttct tcttgcacca
     1861 gcccaggata tcatcggaaa gctcctttat cagctcgggc tggggcatga actcgcaggt
     1921 gatgacggcc agcttctcgc acctctcccc ctttttctcg gagtccctat gcccggcgta
     1981 gatgcggacg ggaggggagg gtacaccgtc atggatgatg gtgaagggcg gaaagtgctt
     2041 ggatatgatg gcggcccggc gctccagcct catcgtgcgg acgatgaagt tggaggcgat
     2101 cgagctcaca aggcccacac tcggaaagcc gatgatggcc atgccgtcct tgaggtccac
     2161 ctctccatac tcgtggatga atatctcgcc ttcgctcatt gggttcactc gcgtgaagag
     2221 gtaggtcagg ggcttaatag ttgacggtgc ccacacccca aaaccattat ccagggaaag
     2281 cgttccaggg ctgtggctga atctgga
//
LOCUS       DAOSYN010000216         2305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_35670_length_2305_cov_2.889778, whole genome shotgun sequence.
ACCESSION   DAOSYN010000216 DAOSYN010000000
VERSION     DAOSYN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35670_length_2305_cov_2.889778"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1747)
                     /locus_tag="PLJ11_07145"
     CDS             complement(95..1747)
                     /locus_tag="PLJ11_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO04477.1"
                     /translation="MPRESITREATIGQLLDETVAKVPDQDAVVYVDRDYRQTWSELR
                     ETVDQVAKGLMALGVRRGEKVAVWATNVPHWLTLQFATARIGAILLTININYRAEEIS
                     YLLRQSECENIFIIDGYRDTDYVNTLYELVPELRTQPRDRLRSERFPHLRRVLFMGPE
                     KHRGMYSWNEVLAMSVAVDDEEYRRRQEGLSPHDVVNMQYTSGTTGFPKGVMLTHFNI
                     TNNGYWVGVNENLTSADRVCLPVPLFHCFGCVLGVMACLNHGATMVILERYDPVNVMM
                     SVEHEKCTALYGVPTMFIGVLDHKLFDRFDLSSLRTGIMAGSPCPVKTMRECVDRMNM
                     REVTIVYGLTEGSPGMTQTRYDEPSLERKCSTVGKAIPGIEVAIIDPETGEHLGPGRH
                     GELCCRGHNVMKGYYNMPEETAKAIDKDGWLHSGDIGMVDEDGYYSVTGRLKDMIIRG
                     GENIYPKEVEDFIHYLEGVADVQVVGVPSVKYGEVPAAFVILRPGARLTAEDIIDHCR
                     GRIAFYKIPRYVHFLEEYPMTASGKIMKVKLREMASSLWPDA"
     gene            complement(1757..>2305)
                     /locus_tag="PLJ11_07150"
     CDS             complement(1757..>2305)
                     /locus_tag="PLJ11_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO04478.1"
                     /translation="KLGTRIRTYRERLGMSVEDLAKNAGVEVSLIMRAEGGEVYPPLG
                     VLLKLSRALGQRLGTFMDDQVRLDPVITRAEERCEETAAHKEGAVGKYHYYPLAKGKT
                     DRHMEPLFIIVQPGADPCPSSHEGEEFVIVVDGEVELVYGKETFHLKEGDSMYYNSVV
                     PHHLGAANGEPAEIYAVVYTPI"
BASE COUNT          378 a          798 c          667 g          462 t
ORIGIN      
        1 gctcgatgcc cttcgcagcc ccatcgaacg ctccgtcctg gctcaggcga cgcgcttcca
       61 tgcgcccccg ctcggacccg ttcgtccgcc ctcgtcaggc gtcgggccac aggctggagg
      121 ccatctccct cagcttgacc ttcattatct tcccgctggc ggtcatgggg tactcctcga
      181 ggaaatgcac atacctgggg atcttgtaga aggcgatccg cccgcggcaa tggtcgatta
      241 tgtcctcggc cgtgagcctc gcccccggcc gcaggattac gaacgccgcc ggcacctccc
      301 cgtacttgac cgaaggcacc cctacgacct gcacgtccgc cacgccctcg aggtaatgga
      361 tgaagtcctc cacctccttg gggtatatgt tctccccgcc ccggattatc atgtccttca
      421 ggcgccccgt caccgagtaa tagccgtcct cgtccaccat tccgatgtcc ccggaatgca
      481 gccaaccgtc cttgtcgatg gccttggcgg tctcctccgg catgttatag tagcccttca
      541 tcacattgtg cccgcggcaa catagctcgc cgtgcctgcc cggcccaagg tgctcccccg
      601 tctccgggtc gatgatcgcc acctctatgc ccggaatggc cttcccgacc gtggagcact
      661 tccgctcgag gctcggctca tcgtacctgg tctgggtcat gcccgggctc ccctccgtga
      721 ggccgtagac gatggtgacc tcccgcatgt tcatgcggtc cacgcactcc ctcatggtct
      781 tcacggggca gggcgagccg gccattatcc ccgttctgag gctggagagg tcgaacctgt
      841 cgaacagctt gtggtccagg acgccgatga acatggtcgg caccccgtac agggcggtgc
      901 acttttcatg ttcgaccgac atcatcacgt tcaccgggtc atacctctcc aatatcacca
      961 tggtcgcccc gtggttcagg caggccatga cgcctaggac gcagccgaag cagtggaaga
     1021 gggggacggg gaggcacacc cggtcggcgg acgtaaggtt ctcgttgacc ccgacccagt
     1081 acccgttgtt ggttatgttg aagtgggtga gcatcacccc cttggggaag ccggtggtgc
     1141 cggaggtgta ctgcatgttg acgacgtcgt ggggggacag cccctcctgc cgccggcggt
     1201 actcctcgtc gtccacggcc acgctcatgg ccagcacctc gttccaggag tacatccccc
     1261 ggtgcttctc agggcccatg aacagcaccc tccggaggtg cgggaaccgt tcggagcgca
     1321 gtcggtcccg cggctgcgtc ctgagctcgg ggacgagctc gtacaacgtg ttaacgtagt
     1381 ccgtgtcgcg gtagccgtcg atgatgaata tgttctcgca ttccgactgc ctaagaaggt
     1441 agctgatctc ctccgcgcga tagttgatgt tgatcgtcag caggatggcc ccgatccggg
     1501 cggtggcgaa ctgcagcgtg agccaatgcg ggacgttggt ggcccacacg gccaccttct
     1561 ccccccgtct gacgcccaaa gccatgagcc ccttggcaac ctggtccacg gtctccctga
     1621 gttccgacca ggtctggcgg taatcacggt ccacgtagac gaccgcgtcc tggtccggaa
     1681 ccttcgcgac cgtctcatcg agcagctggc cgatggtcgc ctcccttgtg atgctctccc
     1741 tgggcatggg atcgcctcat atcggggtat agacgaccgc gtagatctcg gccggctccc
     1801 cgttcgccgc cccgaggtga tgggggacga cggagttgta atacatggaa tcgccctcct
     1861 tcagatggaa cgtctccttc ccatagacga gctctacctc cccatcgacg acgatcacga
     1921 actcctcgcc ctcatgggag gacgggcagg gatcggcgcc cggctgaacg atgatgaaga
     1981 gcggctccat gtgccggtcc gtcttgccct tcgccaacgg atagtaatga tacttgccga
     2041 ccgccccctc cttgtgcgcg gcggtctcct cgcatcgctc ctccgcccgg gtgatgaccg
     2101 gatcaaggcg cacctggtca tccatgaacg tccccaggcg ctgtccgagg gcccgcgata
     2161 gcttcaggag gaccccgagg ggagggtaca cctccccgcc ctccgccctc atgatgaggc
     2221 tgacctcaac gccagcgttc ttcgcaaggt cctccacgct catgcccaac cgttcgcggt
     2281 acgtccgtat gcgcgtccca agttt
//
LOCUS       DAOSYN010000217         2303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_35711_length_2303_cov_3.123665, whole genome shotgun sequence.
ACCESSION   DAOSYN010000217 DAOSYN010000000
VERSION     DAOSYN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35711_length_2303_cov_3.123665"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLJ11_07155"
     CDS             complement(<1..322)
                     /locus_tag="PLJ11_07155"
                     /inference="COORDINATES: protein motif:HMM:NF039705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HOO04479.1"
                     /translation="MRGTNVPNAIYVECPDCGEETLHEVLKGRLGKDGSTLEGTLRCQ
                     ECGRVHPAVVREGRTIKVPVIVSDRRESRRAEVELPEDEELQVEDELQLDDLPIIISS
                     LEREG"
     gene            complement(319..903)
                     /locus_tag="PLJ11_07160"
     CDS             complement(319..903)
                     /locus_tag="PLJ11_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO04480.1"
                     /translation="MSANRVEVLCPGMIRRDGPVVLEARSTATLVSRGGRRFLVDTSG
                     PENREALLEALEARGVPPGGNEAVVLTHAHGDHAGNLSLFPDAKVIAHRLEGGSLALE
                     GEVELWEGARLVPTPGHTPGSISVVVRAEETYALAGDAIPTEDNVRRWVPPGHHYDRE
                     RAMESMALLVGMADVIVPGHGPPFRVEGRKGGGR"
     gene            complement(900..1793)
                     /locus_tag="PLJ11_07165"
     CDS             complement(900..1793)
                     /locus_tag="PLJ11_07165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOO04481.1"
                     /translation="MDPFSLLSMGIIAMGLGLAYGKRYPLAQTLVLIDLAVFLVCAIA
                     AWDHPYLLSPVELELGVRPSSLGDPASWYTIITHMFVHADIMHVLFNMLFLFLVGVPL
                     EERIGRRAFALCFFVPGLLALALEVLVRGLASPVLILGASGAISGTMGALLYLYPRDE
                     IPMFLGPLFLPRVPVWLSVGAWFAVQVVNVLTLPSGVASGGTAYSAHIGGFVAGMALA
                     SLLPGARPKEEGTVDLSELATTDELRELKARIEGESEPEVRKAWLEHFVEKASCPSCG
                     SRPSLEGNRIKCACGWEKRVR"
     gene            1866..>2303
                     /locus_tag="PLJ11_07170"
     CDS             1866..>2303
                     /locus_tag="PLJ11_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOO04482.1"
                     /translation="MQRDPSIVLEVGPGVYPPAEDTFLMLSAMSVGKGERALEMGCGS
                     GLLALHMAKAGAEVTAVDVDGRAVTSTLRNAAANGLRLTVLRSDLFAEVEGEFDLLVF
                     NPPYLRGVAEGEDDLCWAGGPGGTEVTARFLAQAKERLAPGGRV"
BASE COUNT          366 a          826 c          751 g          360 t
ORIGIN      
        1 ccccttccct ctccagcgag ctgatgatga tggggaggtc gtccagctgc agctcgtcct
       61 ccacctgcag ctcctcatcc tccggaagct ccacctccgc cctgcgggac tccctccggt
      121 cgctgactat gactggcacc ttgatggtcc tgccctcccg gaccacggcc ggatgcaccc
      181 ggccgcactc ctggcagcgg agggtaccct ccaaggtgct cccatccttg cccagccttc
      241 ccttgagcac ctcgtggagg gtctcctcgc cgcagtccgg gcattccacg tagatagcgt
      301 tcggcacgtt cgttcctctc acctccctcc tcccttcctt ccctccacgc ggaacggtgg
      361 cccgtggccg ggcacgatca cgtcggccat gccgaccagc agggccatgc tctccatggc
      421 cctctcgcgg tcgtagtgat gcccgggggg cacccatctc cgcacattgt cctcagtggg
      481 tatggcgtcc ccggccaagg cataggtctc ctcggcccgc accaccacgc ttatgcttcc
      541 cggggtgtgc ccgggagtgg ggacgagcct cgccccctcc cacagctcca cctccccctc
      601 cagggcgagg gagccgccct ccaggcgatg ggctatgacc ttggcgtccg ggaaaaggga
      661 caggttgccg gcatggtccc cgtgggcatg ggtcagcact accgcctcgt tccccccggg
      721 gggcacgccc ctcgcctcca gggcctccaa aagggcctcc ctgttctccg ggccgctcgt
      781 gtccaccagg aagcgcctgc ccccccggct caccagagtc gcggtggagc gggcctccag
      841 gacgaccggc ccatcgcggc ggatcatccc cgggcagagc acctccaccc tgttcgcgct
      901 catctcactc tcttctccca tccgcatgcg cacttaatac ggtttccctc caagctgggc
      961 cgggacccgc aggaggggca ggaggccttc tccacgaaat gctcgagcca ggccttccgt
     1021 acctcgggtt cgctctcgcc ctcgatgcgc gccttgagct ccctgagctc gtctgtggtg
     1081 gccagctccg agaggtccac cgtcccctcc tccttgggcc ttgccccggg gagcagggag
     1141 gcaagggcca tgccggccac gaagccccct atgtgcgcgg agtaggccgt cccgccggag
     1201 gcgacccccg aggggagggt gaggacgttg accacctgga cggcgaacca ggcgcccacg
     1261 gacagccaga cgggcacgcg ggggaggaag agcgggccca ggaacatcgg gatctcgtcc
     1321 ctggggtata gatatagaag cgcccccatg gtcccggaga tggccccgga cgcccctagt
     1381 atgagaacgg gggaggcgag gcccctgacc agcacctcca gggccagggc gaggaggccg
     1441 gggacgaaga agcagagggc gaaggcccgc cggcctatcc gctcctccaa cggcactccc
     1501 accaggaaca ggaagagcat gttgaacagc acatgcatta tgtcggcgtg cacgaacatg
     1561 tgggtgatga tcgtgtacca tgaggccggg tccccgaggg agctgggcct gacccccagc
     1621 tccagctcca cgggggacag gagatagggg tggtcccagg cggcgatggc gcagacgagg
     1681 aagaccgcca ggtcgatcag caccagggtc tgcgccaacg ggtaccgctt gccgtaggca
     1741 agcccaagcc ccatggctat gatccccatg ctgagcaggc tgaacgggtc caccttccct
     1801 ccatgccccg ccccgcttta aaggcttcgg gaaaggttaa tagcccgcgc agggctgggt
     1861 cccccatgca acgggatccc tccatcgtcc tcgaggtcgg cccgggcgtg taccccccgg
     1921 cggaggacac gttcctcatg ctctccgcaa tgtccgtcgg gaagggggag agggccctcg
     1981 agatggggtg cgggagcggc ctcctcgccc tgcacatggc caaggccggg gcggaggtca
     2041 ccgcggtgga tgtggacggg agggcggtca cgagcacgct aaggaacgcc gccgccaacg
     2101 gcctgcgcct cactgtcctg cgcagcgacc tgttcgccga ggttgaaggg gagttcgacc
     2161 tcctggtctt caatcccccc tacctccggg gcgtggcgga gggagaggac gacctgtgct
     2221 gggccggggg gcctgggggg accgaggtga cagcccgctt cctcgcccag gcgaaggagc
     2281 gtctagcgcc gggcggcagg gtg
//
LOCUS       DAOSYN010000218         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_35883_length_2293_cov_3.842270, whole genome shotgun sequence.
ACCESSION   DAOSYN010000218 DAOSYN010000000
VERSION     DAOSYN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35883_length_2293_cov_3.842270"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLJ11_07175"
     CDS             <1..631
                     /locus_tag="PLJ11_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO04483.1"
                     /translation="PCIANFPFMPVKPGSMGKALPGVVAAVVDENGLELPPGKEGFLA
                     LRPGWPSMMVGIWNNVTRYREYFYIPGWYSSGDQAYRDEDGYFWFLGRADDVIKTSGE
                     RLGPFEVESALIEHPAVAESAVVGKPDELRGEIVKAFIVLRQGIAPSDRLKEEITNFV
                     RVRLAYYAYPREIEFVEALPKTRSGKIMRRVLKAKEGGHETGDLSMLED"
     gene            complement(837..2036)
                     /locus_tag="PLJ11_07180"
     CDS             complement(837..2036)
                     /locus_tag="PLJ11_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO04484.1"
                     /translation="MSKVLFVSGSVGLGHVQRDVRIARELMALRRDAAVVWLAGDPAT
                     RVLEEEGMVLLPECQERDVGASVIESVARGPRVSMTEVLYRVRKEDRYAQAVRAFDAL
                     VERERPDLVVADEAYELEGAHAQGAGRDWPSFVFLWDFVKVYPGSWRWRDVRTARLVN
                     KGWDRAFRKGREAGWTDLFLGEPDDVPDERLGIGMVNARRGAEGRYHFVGNPLPFDPA
                     GYADRAAVRARLGYGQGRLLICSTGGTAVGADLLRLCLRAVPLMRASAPDLKVLVVKG
                     PRMEADLGEVPPGVEVRGYVPRLFEHFAAADMAIVQGGGTSTLELAALRVPFLYFPLK
                     GHSEQEVNVARKLERNGLGIKCDIARTSPGELASLVLGNIGKVPGPPALSFEGCRNAA
                     RAIASLL"
BASE COUNT          388 a          769 c          747 g          389 t
ORIGIN      
        1 gccgtgcatc gccaacttcc cattcatgcc cgtcaagccg gggtcgatgg gcaaggcctt
       61 gcccggggtg gtggcggcgg tggtggacga gaacggcctg gagcttccgc cggggaagga
      121 ggggttccta gcccttcgcc ccggctggcc ttccatgatg gtcggcattt ggaacaacgt
      181 caccaggtac cgggagtact tctacattcc tggctggtac tcctccgggg accaggccta
      241 ccgggacgag gacggctact tctggttcct ggggcgggcg gacgacgtga tcaaaacatc
      301 tggagagcgc cttgggccgt tcgaggtcga atccgctctc atcgagcatc ccgcggtggc
      361 cgaatcggcc gtcgtcggca agccggatga gctgcgcggg gagatcgtga aggccttcat
      421 cgtgctgcgc caggggatag cgccctcgga ccggttgaag gaggagatca ccaatttcgt
      481 ccgggtaagg ctggcgtact atgcctatcc cagggagatc gagttcgtag aagctcttcc
      541 gaaaacgcgc tcaggcaaga tcatgcgccg ggtgctgaag gccaaggagg gcgggcatga
      601 gaccggggac ctttccatgc tcgaggacta gccgtctttc atctgccctc cggtcatttt
      661 caagggctgc agggtaggcg gtcctggcaa ggcgaggggg agggcaaggc cctctcgatc
      721 cggggatagg acgtggggcg cggccatcag agcgcatgat cttgcgaatg gtgcatgatt
      781 ggggcgacgg ccatgggcca tgccgttcga agagacgcgg gaagagggcc tgggggctag
      841 agcaggcttg cgatcgcccg cgctgcgttc ctgcatccct cgaaggacag ggcgggaggg
      901 cccgggacct tgccgatgtt ccccaagacc agcgatgcca gttccccggg ggatgtccgg
      961 gcgatgtcgc attttatccc gagcccgttc ctctccagct tcctggcgac gttcacctcc
     1021 tgctccgagt gccccttgag cgggaagtac aggaacggca ccctcagtgc ggccagctcc
     1081 aacgtggagg tccccccgcc ctgcacgatg gccatgtccg cggcggcgaa gtgctcgaag
     1141 agccggggga cgtagccgcg gacctcaacc cccgggggga cctcccccag gtccgcttcc
     1201 atgcgcggcc ccttcacgac cagcactttc aggtcgggcg cactcgccct catcaggggg
     1261 accgccctca ggcagagccg gaggaggtcg gcgcccaccg ccgtgccgcc ggtggagcat
     1321 atcaacaggc gtccttgccc atacccgagg cgggcgcgga ccgccgccct gtcagcgtac
     1381 ccggcgggat cgaacggcaa cgggttcccc acgaaatgat agcgcccctc cgccccccgc
     1441 ctggcgttca ccatgcctat ccccaggcgc tcgtcgggaa catcatccgg ctcccccagg
     1501 aagaggtccg tccatccggc ctccctcccc ttgcggaagg cccggtccca ccccttgttc
     1561 acgaggcggg cggtgcggac gtccctccac cgccagcttc cggggtacac tttgacgaaa
     1621 tcccacagga atacgaacga cggccaatcc ctcccggccc cctgggcgtg cgccccctcc
     1681 agctcatagg cctcgtcggc gacgacaagg tccggccgct cccgctcgac cagggcgtcg
     1741 aacgcccgca cggcctgggc gtagcggtcc tccttcctca ccctgtacag cacctcggtc
     1801 atgctcaccc gggggccgcg ggccaccgac tcgatgaccg aggcgccgac atccctttct
     1861 tggcattctg gaagtaggac catcccctcc tcctccagga ccctggtggc agggtccccc
     1921 gccagccaga ccacggcggc gtcgcgcctg agggccatga gctcccgtgc gatgcgcacg
     1981 tccctttgga cgtggccgag gcccacggag ccgctgacga agagaacctt gctcatcagg
     2041 gacctcattc gtctgaatgc tctttagcgt atgcggaggc ttgtccgata ggacggcggg
     2101 gcgatgatgc ggtcgtccct aggtgagata tcatacccgc cgaacgagga ccaaaaccgg
     2161 ttaataggcg ccagggaatg gcatcaagga tgtccgttcc tcgaagggat gtcccctggc
     2221 acctcagaac cgctggggcc ttgatcatgg cggcggcgtt catcgttctc atcggcatca
     2281 taaccgcgga ggc
//
LOCUS       DAOSYN010000219         2290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_35949_length_2290_cov_2.892617, whole genome shotgun sequence.
ACCESSION   DAOSYN010000219 DAOSYN010000000
VERSION     DAOSYN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35949_length_2290_cov_2.892617"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PLJ11_07185"
     CDS             complement(<1..1186)
                     /locus_tag="PLJ11_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO04485.1"
                     /translation="MASRRGVLTGLSTNVLVLGLVSLLTDVSSEMIYPILPLFLTGIG
                     ATGAVIGLIEGAAETTASLLKVVSGRLSDMLGKRKPFLTSGYGLSTVAKPLLFFATSY
                     WHVFAVRILERTGKGLRSAPRDALIADSTDKEIMGKAYGVHKAMDSTGAVIGPLLVVP
                     VLLAAVTVTTDTYRSIFLLATIPAALAVGVLFLFVKEPERKAPRATGRMWKDVNRLGG
                     GFWALIAVVLIFFAGEISYSFFVLQSQAQGMGTIATILLYTLFNAVFVLVSLPSGIYS
                     DRVGRRPILSLSFVLFAVTCLVMASADGLPLLIAGFMLYGVYKGMSEGAFKAFVVDMV
                     PQDLRGTALGVYHTAVGLVMLPGGLVAGLLWDAAGAWGTFGYGMATSVLALALMLFFT
                     RSA"
     gene            1613..1686
                     /locus_tag="PLJ11_07190"
     tRNA            1613..1686
                     /locus_tag="PLJ11_07190"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1647..1649,aa:Ile,seq:cat)
     gene            complement(1736..1972)
                     /locus_tag="PLJ11_07195"
     CDS             complement(1736..1972)
                     /locus_tag="PLJ11_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04486.1"
                     /translation="MKGGTPQKEAGRDNERALLIVSESYGHEDLRQTHQYLELPIDDL
                     NEVQQKRSEYLAKIRNRMREGLPPEKDFSVRISR"
BASE COUNT          441 a          675 c          709 g          465 t
ORIGIN      
        1 cagcgcttct ggtgaagaag agcatgaggg ccaacgccag gacgctggtc gccatcccgt
       61 agccgaacgt gccccacgcc cccgccgcgt cccagagcag gccggccacc aggccgccgg
      121 gcagcatgac cagccccacg gcggtatggt agacgcccag ggcggtgccg cggaggtcct
      181 ggggcaccat gtccacgacg aatgccttga acgccccctc gctcatcccc ttgtacacgc
      241 cgtaaagcat gaaccctgcg atcagaagcg gcagcccatc cgccgatgcc atgaccagac
      301 atgtgacggc gaagaggacg aaggagaggc tcagtatcgg ccttcgcccg acccggtcgg
      361 aatagatgcc cgagggcagc gagaccagca cgaagacggc gttgaagagc gtgtacagca
      421 ggatggtggc tatcgtgccc atgccctgcg cctgcgattg gaggacgaag aaggagtagc
      481 tgatctcccc cgcaaagaag atgaggacga cggcgatcag cgcccagaag ccgccgccaa
      541 ggcgattgac gtccttccac atccggccgg tggccctggg cgccttcctt tccggttcct
      601 ttacgaaaag gaataggaca cccacggcca gggccgctgg gatggttgcc agcaggaaga
      661 tcgaccggta ggtgtcggtg gtgaccgtga cggcggccaa caggacaggg acgacgagca
      721 gggggccgat gaccgccccg gtggagtcca tcgccttgtg caccccgtag gccttcccca
      781 ttatttcttt gtccgtggaa tcggcgatca gcgcgtcgcg cggtgcgctc ctgaggccct
      841 tcccggtgcg ctcgaggatg cgcaccgcga acacgtgcca gtaggatgtg gcgaagaaga
      901 ggagcggctt ggccacggtg ctgaggccat atcccgaggt gaggaagggc ttccgtttcc
      961 ccagcatgtc cgagagcctt ccggacacga ccttcagcag ggaggcggtg gtctccgccg
     1021 ccccttcgat cagccctatg actgccccgg tggcccctat gccggttagg aagaggggca
     1081 atatcgggta gatcatctcg ctgctcacgt ccgtgagcaa ggagacgaga ccgagcacga
     1141 gcacgttggt ggacagcccg gtaagcaccc ctcttctgct agccatccta aaggataccg
     1201 ttcttgggac attaagggct tatcggcctt ctcaatgcgc atgtggaagc gccgagcggg
     1261 gttgcgatgc tcccgccttg gaggcgggcc cgcccgtcaa gatgcggtga ggatggaggg
     1321 cgcgggcttg gcacgggagc gccgcccctc ccgcaatagc catggccatc gggagcaggg
     1381 ggagccgaac ttcgccggca ttccctacgc atatcggcct ggcgatccct gatggatgga
     1441 cggtgcctgc tttgggaatg agcacgttga aaagctcaac gcccatggtc ccaatggatg
     1501 gtccattgcc gtgatctact ctgaggcata tagagaaagg ttctggcgcc cccgactcag
     1561 gatccgaggc atatttttat atcgtttctt gctttcagtt catccgcgaa gcgggtccgt
     1621 agcttagtct ggtggagcga ccggctcata accggttggt cgtcggttca aatccgaccg
     1681 ggcccatcac actcactggc ttttcctatt tttcccctaa ggggtgcaga aaggttcatc
     1741 gggagatcct cacggagaag tccttctccg ggggaagccc ctccctcatc cgattccgaa
     1801 tcttggcgag atattcgctc cgtttctgct gaacctcgtt caaatcgtcg attggaagtt
     1861 ctaagtactg gtgcgtctgc ctgaggtcct cgtgcccgta ggattctgac actatgagca
     1921 gggccctctc gttgtccctg ccggcctctt tttgaggtgt accgcccttc atcgtggccc
     1981 gtgggggtcc gctttatcct cttcagcttc catggggtct ttcgtccgtt ccttggtcca
     2041 gatattcatc gagcgtattc gatagctgtc attatcgctc gctctacctg gcatcaaata
     2101 gattatcatg aatgaaacac aatggaggta catctcactt ggaagatggg ggagaagatg
     2161 gaatggaacc tgtgaaggga tgcctatgaa cttctttggg agaaggagag cgttcgtatc
     2221 gatatccgtc ttcttggtcg tgatggtcgt catctccgcc gggtgcgcat ttttgcagat
     2281 cgcgggggcg
//
LOCUS       DAOSYN010000220         2263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_36496_length_2263_cov_3.837409, whole genome shotgun sequence.
ACCESSION   DAOSYN010000220 DAOSYN010000000
VERSION     DAOSYN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36496_length_2263_cov_3.837409"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="wecB"
                     /locus_tag="PLJ11_07200"
     CDS             complement(<1..1055)
                     /gene="wecB"
                     /locus_tag="PLJ11_07200"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOO04487.1"
                     /translation="MRFSIVLGTRPEMVKMSPVVRELQRRGEEFDLVHTGQHYSFEMD
                     RVFFRDLDLPQPAVNLEVGSGTHGAQTGRMLIGLERTFQACRPDIVLVQGDTNTVLAG
                     ALAAAKMGIAVGHVEAGLRSFDRSMPEEVNRVLADHASDLLFAPTEVSARNLRVEGLP
                     PERIFVTGNTIVDAVRQNLRLAMDRENALRALGLSKGRYALATLHRQENVDSPERLAS
                     IVHGLEATALRTGLDIIWPMHPRARKGLEAFGIGLPRGIRGVPPLGFLEFLRLESEAA
                     LVLTDSGGVQEECCIMGVPCVTLRDSTERPETVDVGANVVVGTEAARIVEGAMVMLAR
                     KGGWSCPLGEEGVAQRI"
     gene            complement(1052..1849)
                     /locus_tag="PLJ11_07205"
     CDS             complement(1052..1849)
                     /locus_tag="PLJ11_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOO04488.1"
                     /translation="MRVVKRSGEMEEFDPSKAIASILKVGTSPEEAEAILEAVRPFLY
                     DGMTTEELYRRIRAHLPSPKAQQYSLKKAILLLGPDGHTFETFVGRLFQDTGYEVLVR
                     QILVGKCISHEVDVVAVKDGRKAIVECKFHNSLGTKSSVQEALYTWGRFLDLEGYDGL
                     AAPWLVTNTKFSSDVVRYAKCVGMNLIGWEYPEDGGLERIIDRTRAYPVTVLNLRRSE
                     QRTLLSHGLVLCRDILERRGELPTLFPREVVERIVRKAEEFQGSVRA"
     gene            complement(1947..>2263)
                     /locus_tag="PLJ11_07210"
     CDS             complement(1947..>2263)
                     /locus_tag="PLJ11_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04489.1"
                     /translation="IVGLPPATCYKLIYQMEQMGLVAHCGAGRNGGRGKATAYTSVLK
                     EMRLEVRSGMIVLTATWKNGTTDEFRKELVFMKAKAEGARAPPASKGADEGLSEREAQ
                     EA"
BASE COUNT          371 a          850 c          635 g          407 t
ORIGIN      
        1 attatccgct gcgctacccc ctcctccccc aacgggcagc tccagccccc cttccttgca
       61 agcatgacca tggcgccctc aacgatgcgc gcggcctcgg tccccaccac gacgttcgct
      121 cccacgtcca cggtctccgg cctctcggtg ctgtcccgca gagtgacgca tggcaccccc
      181 attatgcaac attcctcctg gacgcccccg gagtcggtca gcaccagggc ggcctcgctc
      241 tccaaccgca ggaactccag gaagccgagg ggcgggaccc cccttatccc ccggggcaat
      301 cctatcccga aggcctccag ccccttcctg gcccggggat gcatcggcca tatgatatcc
      361 aaccccgttc tcagggcagt ggcctccagc ccgtgcacga tgctcgccag gcgctccggc
      421 gagtccacat tctcctgcct gtgcagcgtg gcgagggcgt accgcccctt cgacagcccc
      481 agggcacgca gggcgttctc ccggtccatg gccaacctca ggttctgcct caccgcgtcc
      541 acgatcgtgt tcccggtgac gaagatgcgc tcggggggca acccctccac cctcaggttg
      601 cgcgccgaca cctccgtcgg cgcgaacagc aggtccgagg cgtggtccgc cagcacccgg
      661 ttgacctcct cgggcatgga ccggtcgaag gagcgcagcc ccgcctccac gtggccgacg
      721 gcgatcccca tcttcgcagc ggcgagcgcc ccggccagca ccgtgttcgt gtccccctgc
      781 acgaggacga tgtccggcct gcaggcctgg aacgtccttt ccagcccgat cagcatcctc
      841 ccggtctgcg ctccgtgcgt cccagagccg acctcgagat tgacggccgg ctgcggcagg
      901 tccaggtccc ggaagaacac cctatccatc tcgaacgagt agtgctgccc cgtgtgcacc
      961 aggtcgaact cctccccgcg ccgctgcagc tcgcggacca caggggacat cttgaccatc
     1021 tccggccgag tgccgagcac tatgctgaac ctcacgccct cacgcttccc tggaactcct
     1081 cggcctttct gactatcctc tccacaacct ccctggggaa cagcgtgggc agctcgcccc
     1141 tccgctccaa tatgtcgcgg cagagcacga ggccatgaga gaggagggtc ctctgctcgg
     1201 accttctcag gttcaggacg gtcacgggat acgccctcgt ccggtcgatg atccgttcta
     1261 gccctccgtc ctcgggatac tcccacccta tcaggttcat ccccacgcac ttggcgtagc
     1321 gaaccacgtc gctcgagaac ttcgtgttgg tcacaagcca gggggcggcg agcccgtcat
     1381 acccctcaag gtccaggaaa cggccccagg tgtaaagggc ctcctgcacc gaggacttgg
     1441 tcccaagcga attatggaac ttgcactcca cgatcgcctt cctcccgtcc ttgacagcga
     1501 ccacgtccac ctcgtgcgag atgcatttcc cgacgaggat ctggcggacc aggacctcgt
     1561 accccgtgtc ctggaacagc cgccccacga acgtctcgaa ggtgtggccg tcggggccca
     1621 agagcagtat ggccttcttg agggagtact gctgggcctt gggcgacggg agatgggcgc
     1681 ggatgcgccg atagagttcc tcggtggtca tcccgtcata caggaacggc cgcaccgcct
     1741 ccaggatggc ctcggcctcc tcgggggagg tccccacctt aaggatcgag gcgatggcct
     1801 tggaaggatc gaactcctcc atctcaccgg agcgtttgac cactcgcata tccatctcta
     1861 aagcgctgat ggctaatcaa gcttcctggg catccctttc agaccaaccc tgctgtcacc
     1921 ctcgctcgga tgatgacgat ggccgatcag gcttcctgag cctccctctc ggagaggcct
     1981 tcgtccgccc ccttggaggc ggggggcgcg cgggcgccct ccgccttggc cttcatgaac
     2041 accagctcct tacggaactc gtccgtggtc ccgttcttcc aggtcgcggt caggacgatc
     2101 atgccactcc tcacctccag gcgcatctcc ttcagcaccg aggtgtaggc ggtcgccttc
     2161 ccgcgcccgc cgttcctgcc cgcgccgcaa tgggccacca gccccatctg ctccatctgg
     2221 tagatgagct tgtagcaggt cgccggcggt aggcccacaa tcg
//
LOCUS       DAOSYN010000221         2255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_36662_length_2255_cov_3.925000, whole genome shotgun sequence.
ACCESSION   DAOSYN010000221 DAOSYN010000000
VERSION     DAOSYN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36662_length_2255_cov_3.925000"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLJ11_07215"
     CDS             <1..612
                     /locus_tag="PLJ11_07215"
                     /inference="COORDINATES: protein motif:HMM:NF017570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF835 domain-containing protein"
                     /protein_id="HOO04490.1"
                     /translation="SLMRKRYDAYEASCLSDGAEKCVHVLTPSTTYPADRMDATKREG
                     ERGRKYQLEPGTAYLVESSSLDAAYQMYRDQIEDGCTGMVLAREYPEKVQRLYGIKEG
                     AILWLSYERGKRYAREPTDIPLIYSEIKNFFEATGRAVVLLSGLEYLISQNNFLKVLK
                     LLQLLNDNVSLTGATLIIPVVPEALNPQDVKMLERELRVLEVD"
     gene            653..1123
                     /locus_tag="PLJ11_07220"
     CDS             653..1123
                     /locus_tag="PLJ11_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HOO04491.1"
                     /translation="MNLVPKKFFVTQGAAVSKVSDLNAFDEALMKAHIGELNLVSVSS
                     VLPVGAKKVAVRELPMGAITHCVLAQMRGGEGEMISAGIAYAFRKDGKGGYVAEGHMH
                     GTKKALKEVLEWKMNEMSTLRGIKLMRINYAIEELSIPMDHYGTCLGALVFVEY"
     gene            1123..1518
                     /locus_tag="PLJ11_07225"
     CDS             1123..1518
                     /locus_tag="PLJ11_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04492.1"
                     /translation="MYAAVVCPRCRKGFAVDLEHRTSSCPHCGRQVRTGRMRARFTSP
                     SAVLTAQAVAELNRGGRALKGFHGKVVEEGETAPPLPLEELIKGLGEFDVRGLEAALR
                     GRMDAEEALRRLLAVGMVYEVSPGRYRMA"
     gene            complement(1515..>2255)
                     /locus_tag="PLJ11_07230"
     CDS             complement(1515..>2255)
                     /locus_tag="PLJ11_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOO04493.1"
                     /translation="DTMVWEGEGLTGMFTVQMTIEVAQARHVWELDEGSVLDRDEVPQ
                     YYLDNYLDDQWKIVVNDPAIRSLREELVGDETNVYLIAKAIYDWIVENIEYPDSPSPG
                     EPRSSVETLRAREGDCDEQSMLFCALARSAGVPAWVQLGAIYDRKDEELGGHGWVQMF
                     MPTADGGHNVTIDPVNEAFLVWMPTLFCEYTDTGDADDLYSAYHHISISFLPEGATPI
                     YTEGWRVIAYEEGEGRASFASTIVRGPS"
BASE COUNT          422 a          682 c          729 g          422 t
ORIGIN      
        1 tccctcatgc gcaagcgcta tgatgcctac gaggcgtcct gcctctccga tggggcggag
       61 aagtgcgtgc atgtgctcac gccctccacc acctatccgg cggataggat ggatgccacc
      121 aagagggagg gggagagagg gcgcaagtac cagctggagc cggggacagc ctacctggtg
      181 gagtcgtcgt ccctcgatgc agcgtaccag atgtatcggg accagatcga ggatggctgc
      241 accggtatgg tgctcgcccg tgagtatccg gagaaggtac agaggctgta cggcatcaag
      301 gagggggcca tcctctggct ttcgtacgag aggggcaaga ggtatgccag ggagccgacc
      361 gatatccccc tgatctatag cgagatcaag aacttcttcg aggccaccgg gcgcgcggtg
      421 gtgctgttgt ctggcctgga atatctgata agccagaaca acttcctcaa ggtgctcaag
      481 ctcctgcagc tcctgaacga caacgtctcc ctgaccggcg ccacgctcat catccctgtc
      541 gtgcctgagg cgttgaaccc ccaggacgtc aagatgctgg agcgtgagct gcgcgtgctc
      601 gaggtcgact gaaggcaaac cttaaatcag gattgcgatt tacatcgcaa ttatgaattt
      661 ggtccctaag aaatttttcg tcacccaggg agcggccgtc agcaaggttt ccgacctcaa
      721 cgccttcgac gaggcgttga tgaaggcgca catcggagag ctgaacctgg tgtccgtatc
      781 ctccgtcctg cccgtggggg ccaagaaggt cgcggtgcgg gagctgccca tgggcgccat
      841 cacccattgc gtcctggccc agatgagggg cggcgagggg gagatgatct ccgctgggat
      901 cgcctacgcc ttccgcaagg acggcaaggg cggttatgtc gctgaaggac atatgcacgg
      961 gaccaagaaa gcgttgaagg aggtgctgga gtggaagatg aacgagatgt ccaccctgcg
     1021 cgggataaag ctgatgcgca tcaactacgc catcgaggag ctctccatcc ccatggacca
     1081 ttacggcacc tgcctcggcg cgctcgtgtt cgttgagtac tgatgtacgc tgccgtggtc
     1141 tgcccacggt gccgcaaggg cttcgcagtg gacctggagc atcggacctc ctcgtgcccg
     1201 cactgcgggc gccaggtccg cacggggcga atgagggcgc gtttcacctc cccctcggcg
     1261 gtcctcaccg cccaggcggt ggcggagctg aaccgcgggg ggcgcgccct gaagggcttc
     1321 catggcaagg tggtggagga aggggagacc gccccgccgc tcccgctgga ggagctgatc
     1381 aaggggctgg gggagttcga cgtcaggggg ctggaggcgg ccttgcgggg gaggatggac
     1441 gccgaggagg ccttacgccg gctgctggcc gtgggcatgg tctacgaggt ctcccccggc
     1501 cggtaccgga tggcctagga ggggcccctg acgatcgtgg aggcgaagga tgcccttccc
     1561 tcgccctcct catatgcgat caccctccat ccctccgtgt atatcggagt ggccccctcg
     1621 ggcaggaagg aaatgctgat gtggtgatag gcgctgtaaa ggtcgtccgc gtcccctgtg
     1681 tccgtatact cgcagaagag ggtgggcatc cacacaagga aggcctcgtt gacggggtcg
     1741 atggtcacgt tgtgcccccc atcggccgtg ggcatgaaca tctggaccca tccgtgcccg
     1801 ccgagctctt catcctttct gtcatagatc gccccgagct gcacccaggc cggcaccccg
     1861 gccgaacggg ccagggcgca gaacagcatc gactgctcat cgcagtcccc ctccctggcc
     1921 cggagcgtct caacggagga cctgggctcc cctggcgagg ggctgtcagg atattcgatg
     1981 ttctccacga tccaatcgta gatggccttg gcgatcaggt acacattggt ctcatcgccc
     2041 accaattcct cgcggaggga tcggatggcg ggatcgttca cgactatctt ccattgatcg
     2101 tccagatagt tgtccagata gtattggggg acctcgtccc tatcgagcac gctcccctcg
     2161 tcgagctccc agacatgccg ggcctgagcg acctcgatgg tcatttgcac ggtaaacatg
     2221 ccggtgaggc cctccccctc ccagaccatt gtatc
//
LOCUS       DAOSYN010000222         2250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_36773_length_2250_cov_2.603645, whole genome shotgun sequence.
ACCESSION   DAOSYN010000222 DAOSYN010000000
VERSION     DAOSYN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36773_length_2250_cov_2.603645"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLJ11_07235"
     CDS             complement(<1..427)
                     /locus_tag="PLJ11_07235"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176800.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOO04494.1"
                     /translation="MRKDIKFTLGIDDIPKQWYNIAADSPVPLPPPLNPVTRQPMDPK
                     DLEAIFPRNLIEQEMSQERYIDIPDELREAYIMLNRPSPLVRAVRLEKMLKTPAKIFY
                     KREDLSPCGSHKPNTAVPQAYYNMLAGTEHITTETGAGQW"
     gene            659..892
                     /locus_tag="PLJ11_07240"
     CDS             659..892
                     /locus_tag="PLJ11_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04495.1"
                     /translation="MRYGATTRLSPERALEKAQEYFGKLGLALEEKTERHALFRNDAG
                     CVRVAVATEHGTELEIETKGFDRQVKQFLQRIG"
     gene            complement(893..1759)
                     /locus_tag="PLJ11_07245"
     CDS             complement(893..1759)
                     /locus_tag="PLJ11_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04496.1"
                     /translation="MSELLELLRKVADDGTSGSSEIYSKLLDGLLGALAERPTVPEWR
                     EFSDGLAKVKRSMAPLQNVAGSIRVLLNAQIPEEQFNGFMRGFLNDLAERERLAPSRI
                     AEGLLSSYRPAKVVTLSYSGTVLRSLMALPRGTAVSVAESLPMGEGAITCQRLIGNGI
                     DAKLFRDSMIVRETASADIALVGADGVCPEGVVNKVGTYPLALAAKECGKPFVVLCST
                     SKLIPVLEMDPGDTETERDGLRLKETVFELTPLSLVTAIVTEEGVLSGEEMRQRLLRD
                     RGNMKVTECQSC"
     gene            complement(1813..>2250)
                     /locus_tag="PLJ11_07250"
     CDS             complement(1813..>2250)
                     /locus_tag="PLJ11_07250"
                     /inference="COORDINATES: protein motif:HMM:NF017570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF835 domain-containing protein"
                     /protein_id="HOO04497.1"
                     /translation="KGQYCEEHQAQHEMLRRELRKGRKALYISKNPPRQLQVQLEPVK
                     ENMTALWLSPRPEEDCIPPMNLESFEARITEFLRENESSIVVLNGVDVLCSWNGLRPV
                     IESLKRARGRLGKAELFISLDPSEQNPGYVGALERISDEVVCT"
BASE COUNT          422 a          687 c          690 g          451 t
ORIGIN      
        1 cccattgccc cgccccggtc tcggtggtta tatgctccgt cccggcgagc atgttgtagt
       61 aggcctgagg gaccgccgtg ttcggcttat gggagccgca ggggctcagg tcctccctct
      121 tgtagaagat cttggctggt gtcttgagca tcttctcgag cctcaccgcc ctcaccaacg
      181 gggagggacg gttgagcatg atgtacgcct ccctgagctc gtcggggatg tcgatgtacc
      241 gctcttggga catctcctgc tcgatcaggt tacgggggaa gatggcctca aggtccttgg
      301 ggtccatcgg ttggcgggtc actgggttca agggaggggg gagaggcacc ggggagtccg
      361 cggctatatt gtaccactgc ttcggtatgt cgtcgatgcc cagggtgaac ttgatgtcct
      421 ttcgcatggg agagggagag cggtaattcg atatatatca cttatgggta ttattgtaca
      481 gaaatataca atatcacccc tgccaaggag ggattgacca tgcgatcccc tcacggcacg
      541 cggagggctc gccctccttt cgaccctcat tgaccgatgc ttccctgggc aacatgcggg
      601 gcgggccttt cagggcataa aatagttatt tcctggatac gttgtccaca gggatcgcat
      661 gagatatgga gcaaccacaa ggctgagccc ggagagggcc ctggagaagg cccaggagta
      721 cttcggaaag ctggggctgg cattggagga gaaaacagag cgtcacgccc tgttccgaaa
      781 cgacgcgggg tgcgtgaggg tggccgtcgc aacggagcac ggcacggagc tggagatcga
      841 gaccaaaggc ttcgatcggc aggtcaagca gttccttcag cggatcggtt gatcaacagc
      901 tctggcactc ggtcaccttc atattgccgc ggtccctcag cagccgttgg cgcatctctt
      961 ccccggagag gacgccctcc tcggtgacga tcgctgttac caaggagagg ggcgtaagct
     1021 caaagaccgt ttccttgagc ctcaggccat cgcgctccgt ttccgtgtcc ccagggtcca
     1081 tctccaggac cgggatgagc ttggaggtgg agcagaggac gacgaagggc ttcccgcact
     1141 ccttcgccgc cagggccaaa gggtacgtgc ccaccttgtt caccaccccc tccgggcata
     1201 cgccatccgc cccgacgagc gcaatgtcgg cggaagcggt ttcccggacg atcatggagt
     1261 cccggaagag cttggcgtcg atgccattcc cgataagcct ctggcaggtt atcgcccctt
     1321 cccccatcgg cagcgattcg gccaccgaga ccgctgtccc ccttggcagc gccatcaggg
     1381 agcgcagcac ggtaccggaa taggaaaggg tgacaacctt cgcgggccga taggaactga
     1441 gaagcccctc ggctattctg gagggggcta agcgctcccg ctccgccagg tcgttcagga
     1501 agccccgcat gaagccgttg aactgctcct cggggatctg cgcgttcagc agcacccgga
     1561 tggagccggc cacgttctgc aagggggcca tggacctctt gaccttggcc aggccatccg
     1621 agaattcccg ccactccgga accgtcggcc tttccgccag ggcgcccagg agcccatcga
     1681 gcagcttgga atagatctcg gacgagccgg aggtcccgtc gtcagccacc ttccgcagga
     1741 gctccaagag ttcggacatg gagcagggga aagcgtatgg gctctaaaaa ggatgggctc
     1801 aatgtttccg gctcaggtgc acaccacctc gtccgagatg cgctcaagcg cgccaacata
     1861 gcccgggttc tgctcgctgg ggtccaggct tatgaacagc tccgccttgc cgagccgtcc
     1921 tctggcccgc ttcaaggact ctatgaccgg gcgcagcccg ttccaggagc acagcacgtc
     1981 cactccgttc agcaccacga tcgaggattc gttctccctc aggaactcgg ttatccgggc
     2041 ctcgaagctc tcgaggttca taggagggat gcaatcctcc tccggccggg gggacagcca
     2101 tagggcggtc atgttctcct tgaccggttc gagctgcacc tgcagctgcc tcggcggatt
     2161 cttggagata tacaacgcct tccgcccttt cctaagctcc cgcctgagca tctcgtgttg
     2221 cgcttgatgt tcctcgcagt attggccctt
//
LOCUS       DAOSYN010000223         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_37023_length_2237_cov_3.780477, whole genome shotgun sequence.
ACCESSION   DAOSYN010000223 DAOSYN010000000
VERSION     DAOSYN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37023_length_2237_cov_3.780477"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1594)
                     /locus_tag="PLJ11_07255"
     CDS             complement(68..1594)
                     /locus_tag="PLJ11_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO04498.1"
                     /translation="MGDIGRVFGNRVMELRFRTGEAEDLQVGEMLVVEEEGARFLVRT
                     MDVLYGADSEDEGYLERQAGAALRGGLDYERVKGDLYKVGVCTPLGCLKEGEYRKAKS
                     IPRHFSAVRRADQRDYDFLRPMMGDVEVGRLRSGDRVLDFRVGVASRSFPHHVGIFAT
                     TGMGKSNLMKSLALACMESRRCGFLILDPHGEYFDGGERGKKGLTHSKLAPEALDVYS
                     SRRLDGPHSSIHLSSAEIEIPDLANLYEFSGAQLECLQASQWKYGERWLEELRDRSVA
                     AIVSDLNGRFHEGTVNVIKRRLDSIFQFDLVTTDRNLSMTRAVIAALQGGKTVLVDTS
                     GMFEAEELLVSTVLARAVFEHNKAMYSDKPKFERAPPVLIAMEEAQRVLTQSKGTVFA
                     QIAREGRKFKVGLCAVSQQPKLISEEVLSQFNTLFILGLADKRDRDILRNSAKQDISM
                     LDNEVQMLMPGEALIASPFTPFAIPCRVHLYEERVEESNARAGERGSKVKRDTEQTFF
                     "
     gene            1679..>2237
                     /locus_tag="PLJ11_07260"
     CDS             1679..>2237
                     /locus_tag="PLJ11_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04499.1"
                     /translation="MRPDIVELPSGPSLPRPGLLVVGVGGAGRHMAAQMPGERLTICQ
                     EGDTGIPGQRQVLLRKGELAVARTSSATALHVSDLPWKARLVDAIGRPDVMLLFSGMG
                     GETGSCIAPLIAELAPFTTLTFASVCLPFSVEGPNRRSMAREGLTRLRGAAHLVAVYA
                     NDPLIRLAPNLPLAKAFKVMDQIMLA"
BASE COUNT          341 a          794 c          722 g          380 t
ORIGIN      
        1 gctccccctg ttggatggga aacggtcacc ggtccaaggg caccgccgtt ccttccccgc
       61 gaggggctca gaagaacgtc tgctccgtgt cccgcttcac cttgctcccc ctctcccccg
      121 cccgggcgtt ggactcctcc acgcgctcct cgtagagatg gacccggcac gggatggcga
      181 agggggtgaa cggcgaggcg atcagcgcct ccccaggcat gagcatctgg acctcgttgt
      241 cgagcatgga gatgtcctgc ttcgccgagt tccgcaggat gtcccggtcg cgcttgtccg
      301 ccagccccag gatgaagagg gtgttgaact gggaaaggac ctcctcgctg atgagcttag
      361 gctgctgcga aacggcgcac agcccgacct tgaacttgcg cccctcccgg gctatttggg
      421 cgaacaccgt gcccttcgat tgcgtcagca cgcgctgcgc ctcctccatg gcgatgagca
      481 cgggcggcgc ccgctcgaac ttgggcttgt cggagtacat ggccttgttg tgctcgaaca
      541 ccgcccgggc gaggacggtc gagaccagca gctcctcggc ctcgaacatg ccggaggtgt
      601 cgacgagcac ggtcttgccg ccctgcagcg ccgcgatcac cgccctggtc atgctcaggt
      661 tgcggtccgt ggtgacgagg tcgaactgga atatggagtc gaggcggcgc ttgatgacgt
      721 tcacggtgcc ctcgtggaac ctcccgttga ggtcgctcac aatggccgcc acgctccggt
      781 cccgaagctc ctccagccat cgctccccgt acttccactg ggacgcctgc aggcattcca
      841 gctgcgcccc ggagaactcg tagaggttgg cgaggtccgg gatctcgatc tccgcgctgg
      901 agaggtggat ggagctgtgc gggccgtcca gcctgcggga cgagtatacg tcaagggcct
      961 cgggcgccag cttggagtgc gtcagcccct tcttgccccg ctccccgccg tcgaagtact
     1021 ccccgtgcgg gtcgaggatg aggaagccgc accgcctgga ctccatgcac gccagggcaa
     1081 gcgacttcat caggttgctc ttccccatgc cggtcgtggc gaatatcccc acgtggtgcg
     1141 ggaaggagcg cgatgccacg cccacgcgga aatcgagcac gcggtccccg gagcgcagcc
     1201 tgccgacctc cacgtccccc atcatcggcc tcaggaaatc gtagtcgcgc tggtccgccc
     1261 tgcggacggc ggagaagtgc cgggggatgg acttggcctt gcggtactcc ccctccttga
     1321 ggcagcccag gggcgtgcac acgcccacct tgtagaggtc ccccttgacg cgctcgtagt
     1381 cgagcccgcc cctgagggcc gcgcccgcct gccgctccag gtacccctcg tcctcggaat
     1441 cggccccgta gagcacgtcc atcgtccgca cgaggaagcg cgccccctcc tcctcgacga
     1501 cgagcatctc ccccacctgg aggtcctccg cctccccggt gcggaagcgc agctccatga
     1561 ccctgttccc gaacaccctg ccgatgtcgc ccatgtgccc aggccatgcc ccgaaggggt
     1621 catagtagtt tctccgggca cggtgaaaat gatatccgag gagggcgtta ctcggtccgt
     1681 gcggcctgac atcgtcgagc tcccgagcgg tccctccctt ccccggccag ggctactggt
     1741 ggtcggcgtc gggggggcgg ggaggcacat ggccgcgcag atgccagggg agcggctcac
     1801 catctgccag gaaggggata ccggcatccc cgggcaacgc caggtgctcc tccgcaaggg
     1861 cgagctggcg gtggcccgca ccagttcggc gaccgccctg cacgtgtccg acctcccttg
     1921 gaaggcccgg ctggtggacg ccatcggcag gccggatgtc atgctcctgt tcagcggcat
     1981 gggaggggag accggaagct gcatcgcccc cctcattgcc gagctggccc ccttcaccac
     2041 ccttaccttc gccagcgtct gcctcccgtt ctcggtggag gggccgaacc ggcgctcgat
     2101 ggcccgggag gggctcacgc ggctgagggg cgcggcgcac ctggtggcgg tgtacgcgaa
     2161 cgaccccctg ataaggctgg ccccgaacct tcccctggcc aaggccttca aggtcatgga
     2221 ccagatcatg ctcgccc
//
LOCUS       DAOSYN010000224         2236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_37035_length_2236_cov_4.045851, whole genome shotgun sequence.
ACCESSION   DAOSYN010000224 DAOSYN010000000
VERSION     DAOSYN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37035_length_2236_cov_4.045851"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLJ11_07265"
     CDS             <1..741
                     /locus_tag="PLJ11_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOO04500.1"
                     /translation="QGLLVPGGKVIEPTSGNTGIGLAMVCAAKGYDLVLTMPEDMSVE
                     RVKLLRSLGAEVVLTPAEEGMTGAIARAKALQASGGHYMPDQFNNPANVEAHYLTTGP
                     EVLADLPDIGAFVAGIGSGGTITGVGRFLKERKDVLIVGVEPAGSPVLSGGRPGRHAI
                     QGIGAGFVPSILDLKVVDRVVPVTDEDALRTARALARREGIMAGISSGAALWAGLRLS
                     QELEGKRKIAVLLPDSSERYMSTALFQE"
     gene            745..1404
                     /gene="cysE"
                     /locus_tag="PLJ11_07270"
     CDS             745..1404
                     /gene="cysE"
                     /locus_tag="PLJ11_07270"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOO04501.1"
                     /translation="MSWKEDVRTVLERDPATRSFREALFFSPGLHAILLQRVSHRQYL
                     KGNFGLARAINYFARFLTGADIHPGATIGKGLFIDHAVGVVIGETTVIGDNVSIFQGV
                     TLGGVSTSKGKRHPTIGDNVTIGAGAKVLGNITIGDNVKIGAGSVVIKDVPPNSTVVG
                     VPGRVVRREGAAVKVDLRHDELPDPLRDAMMQLSNSIAELDHRLERLERLVGQEKEGK
                     G"
     gene            1401..>2236
                     /gene="cysS"
                     /locus_tag="PLJ11_07275"
     CDS             1401..>2236
                     /gene="cysS"
                     /locus_tag="PLJ11_07275"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOO04502.1"
                     /translation="MTLQIYNTMAKAKQEFRPIEDKKVKMYVCGVTVYDDVHMGHARF
                     MVVFDMVARYLRYRGYDVTLVINFTDVDDKIIARANEEGTDPLAIAQRYIDAYFRDSD
                     ALRVRRADAYPRASETIPEIIAMVQEIVAAGFGYVAADGSVYFDVSKAPEYGKLSGNR
                     LEDMQAGARIEVDAAKRNPMDFCLWKAAKPGEISWPSPWGPGRPGWHIECSAMVRKLL
                     GETIDIHGGGNDLIFPHHENEILQSEAVTRRPLANYWMHNGMLQVADAKMSKSLRNFF
                     SV"
BASE COUNT          411 a          712 c          794 g          319 t
ORIGIN      
        1 caaggcctcc tcgtcccggg agggaaggtc atcgagccca cgtccgggaa caccggcatc
       61 gggctggcga tggtctgcgc ggccaaggga tacgacctcg tcctcactat gccggaggac
      121 atgagcgtcg agcgcgtcaa gctcctccgc tcgttgggcg cggaggtggt cctcaccccg
      181 gcggaggagg ggatgaccgg ggcgatcgcc cgggcgaagg cgctccaggc ctccgggggc
      241 cactacatgc cggaccagtt caacaacccg gcgaacgtgg aggcgcacta cctcaccacc
      301 gggccggagg tcctcgcgga cctgccggac atcggcgcct tcgtggccgg catcggctcc
      361 ggggggacga tcaccggcgt cgggcgcttc ctcaaggaga ggaaggacgt gctgatcgtc
      421 ggcgtggagc ccgccggctc ccccgtgctc tccgggggaa ggccggggag gcacgccatc
      481 cagggcatcg gcgccggctt cgtgccctcc atcctggacc tcaaggtggt ggaccgcgtc
      541 gtccccgtca cggacgagga cgccctccgg accgcccgcg ccctcgcccg ccgggagggc
      601 atcatggccg ggatctcgtc gggcgcggcc ctctgggccg ggctgaggct aagccaggaa
      661 ctcgaaggga agaggaagat agcggtgctg ctcccggact cctcggaaag gtatatgagc
      721 acagcgctgt ttcaagagta ggccatgagc tggaaggagg acgtgcggac ggtcctggaa
      781 agggacccgg ccaccaggtc gttccgggag gccctcttct tcagcccggg gcttcacgcc
      841 atcctgctcc agcgcgtctc gcaccggcag tacctcaagg gcaacttcgg cctggcgcgg
      901 gcgatcaact atttcgcccg gttcctgacc ggggcggaca tacatccggg ggcgacgatc
      961 ggcaagggcc tcttcatcga ccacgccgtc ggcgtggtca tcggggagac gacggtcatc
     1021 ggcgacaacg tgtccatatt ccagggcgtc acgctggggg gcgtgagcac cagcaagggc
     1081 aagcgccacc cgaccatcgg cgacaacgtg acgatcgggg cgggggcgaa ggtgctcggc
     1141 aacatcacga tcggggacaa cgtcaagata ggcgccggct ccgtggtgat caaggacgtc
     1201 ccgcccaact ccacggtcgt cggcgtcccc ggccgcgtcg tgcggaggga gggggcggcg
     1261 gtcaaggtgg acctgcgcca cgacgagctg cccgaccccc tgcgggacgc gatgatgcag
     1321 ctgagcaaca gcatagcgga gctggaccac cgccttgagc ggctggaaag gcttgtggga
     1381 caggaaaagg agggaaaggg atgacgctgc agatctacaa caccatggcc aaggccaagc
     1441 aggagttccg gccgatcgag gacaaaaagg tcaagatgta cgtctgcggc gtaacggtct
     1501 acgatgacgt gcacatgggg cacgcccgct tcatggtggt gttcgacatg gtggcccggt
     1561 acctccgtta ccgcggctac gacgtcacgc tcgtaatcaa cttcacggac gtggacgaca
     1621 agatcattgc ccgggcgaac gaggagggga cggaccccct ggccatcgct caaaggtaca
     1681 tcgacgcgta cttccgggac tcggacgccc tgcgggtgag gcgggcggac gcctacccca
     1741 gggcgagcga gacgatcccg gagatcatag cgatggtcca ggagatcgtc gccgcgggct
     1801 tcgggtacgt cgccgcggac ggcagcgtct acttcgacgt ctccaaggcg ccggagtacg
     1861 ggaagctgtc ggggaacagg ctggaggaca tgcaggcggg cgcgcggatc gaggtcgacg
     1921 ccgccaagcg caatcccatg gacttctgcc tgtggaaggc ggcgaagccg ggggagatct
     1981 cctggccctc gccctggggc cccggccgcc ccggatggca catcgagtgc tcggccatgg
     2041 tccgcaagct gctcggggag acgatcgaca tccatggcgg gggcaacgac ctcatcttcc
     2101 cgcaccacga gaacgagatc ctgcagtccg aggcggtcac caggcggccg ctggcgaact
     2161 actggatgca caacgggatg ctccaggtgg cggacgccaa gatgtccaag tccctgagga
     2221 acttcttctc ggtgag
//
LOCUS       DAOSYN010000225         2235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_37064_length_2235_cov_3.885780, whole genome shotgun sequence.
ACCESSION   DAOSYN010000225 DAOSYN010000000
VERSION     DAOSYN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37064_length_2235_cov_3.885780"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLJ11_07280"
     CDS             <1..641
                     /locus_tag="PLJ11_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04503.1"
                     /translation="RRFGARFLVGADGWNSLVRRTFFPGGPRLLWAEQFVLAERSEED
                     AMHFFYDERYRGGYRWVFPHRSGSRVGFTRGADERPKCLERHGRPIPYAYGGTVMGNA
                     CLIGDAGGQANPITFGGIRIGMMAARMVAESLAKGDLADYARRWPRSPYASPVYIKAF
                     ETLQGMDNEELYRSVRPFRDGYGRMAGLRALLNGRRYLGLYRAYDLGAKWGW"
     gene            643..1842
                     /locus_tag="PLJ11_07285"
     CDS             643..1842
                     /locus_tag="PLJ11_07285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO04504.1"
                     /translation="MLDCDVLVVGAGPAGSGAARASALQGARTLMVDKKKEIGTPVQC
                     GEVIGLELVKRSGMRIPESAIVARHAFTRFVLGREVIIDNHEPYWRAVTVERKIFDKH
                     LAFRAASAGAAVQADTRLLSVRKDGERVAGCMLSHHGEEMEVSPKAIVAADGVHSTLA
                     KELGVEFFSPKDVARGVEFELVAKRDIPQAMQIFIEEEIGLGYGWIIPKGKRRANVGL
                     GLVGTNASRRTFLEEWITGHPVVSRYFDVDKVLEVKMGDAPVPGFLGGPVRGNVLFCG
                     DAAGQTLAFVGEGIMPSYICGGLAGKHAALLAQGKGERYEEELVENMADQLMMGAALR
                     DALVELWSSGPYDGRARALLSGAVMNELVSPDALDDVIGPMVADPLRTFRDLAKGSGM
                     TVRVRRC"
     gene            2044..>2235
                     /locus_tag="PLJ11_07290"
     CDS             2044..>2235
                     /locus_tag="PLJ11_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6e family ribosomal protein"
                     /protein_id="HOO04505.1"
                     /translation="MVEFKAVVNDMKTGRSYSVPVSGHHANSLIGKKIGDTIDGIFVG
                     LPGYKLTITGGSDKDGFAMR"
BASE COUNT          383 a          658 c          825 g          369 t
ORIGIN      
        1 ggaggaggtt cggcgcaagg ttcctggtgg gagcggacgg ctggaattcc ctggtcagga
       61 ggacgttctt cccgggcggt ccccgcctgc tctgggccga gcagttcgtc ctggcggaga
      121 ggagcgagga ggacgccatg cacttcttct acgacgagag gtaccgggga gggtatcgct
      181 gggtgttccc ccaccgctcc ggaagccggg tcggcttcac ccggggcgcg gacgagagac
      241 cgaagtgcct ggagaggcac ggccggccga tcccctacgc ctatggcgga acggtcatgg
      301 gcaacgcatg cctgatcggg gatgcggggg gtcaggccaa cccgatcacc ttcggcggca
      361 tccgcatcgg gatgatggcc gccaggatgg tcgccgagtc cttggccaag ggcgatcttg
      421 cggactacgc gcgcagatgg ccgcgctctc cgtacgcctc gcccgtctat attaaggcgt
      481 tcgagaccct acagggcatg gacaacgagg agctgtaccg ttcggtgcgg ccgttccgcg
      541 atgggtacgg ccggatggcg gggctgaggg ccctcttgaa cggaaggagg tacctgggcc
      601 tgtaccgtgc ctacgacctg ggggccaaat gggggtggtg acatgttgga ctgtgatgtg
      661 ctggtggttg gtgccggtcc ggccggcagt ggggcagctc gggcctccgc gctgcagggc
      721 gcaaggacgc tgatggtgga caagaagaag gagataggga cgccggtgca gtgcggggag
      781 gtcatcgggc tggagctggt gaagcgctcc gggatgcgca tccccgagag tgccatcgtc
      841 gccaggcacg ccttcacccg cttcgtgctg ggaagggagg tcatcatcga caatcacgag
      901 ccgtactggc gggcggtgac cgtggagcgg aagatattcg acaagcacct ggccttcagg
      961 gcggcctccg ccggggcggc ggtccaggcg gacaccaggc tgctgtcggt aaggaaggac
     1021 ggggagcgcg tcgcaggctg catgctctcc catcacggcg aggagatgga ggtgtccccg
     1081 aaggccatag tggccgcgga cggggtgcac tccacgctcg ccaaggagct cggcgtcgag
     1141 ttcttctccc ccaaggacgt ggccaggggc gtggagttcg agctggtggc caagcgggac
     1201 atcccccagg ccatgcagat cttcatcgag gaggagatcg ggctggggta cggatggata
     1261 atccccaagg ggaagaggag ggcgaacgtc gggctcggcc tagtggggac caacgcctcc
     1321 cgccgcacct tcctggagga atggataacg ggccaccccg tcgtgtcccg gtacttcgat
     1381 gtggacaagg tgctcgaggt caagatgggg gacgcccccg tccccgggtt cctcggcggc
     1441 ccggtcaggg ggaacgtgct cttctgtggg gacgccgccg gccagaccct ggccttcgtg
     1501 ggcgagggca taatgccctc ctacatctgc ggcgggctcg cgggcaagca cgccgccctc
     1561 ctggcccagg gcaaggggga gaggtacgag gaggagctgg tggagaatat ggccgaccaa
     1621 ctgatgatgg gggcggcgct cagggacgcc ctggtggagc tgtggtcctc gggcccctat
     1681 gacgggaggg cccgcgccct cctgtccggg gcggtgatga acgagctggt ctcccccgat
     1741 gccctggatg atgtcatcgg gcccatggtc gccgaccccc tgcgcacctt ccgggacctc
     1801 gccaagggca gcgggatgac ggtgagggtc aggcggtgct gagccccctg ctgcaccgcg
     1861 gcgagcgcgc aggggcggac ggtcctagac ccgagtaacg tctcctttga aacgcccggg
     1921 cgccttggtc cccttccacc cagtggtcgg tgcggcccct gggggcgcgc cccccgtacc
     1981 aataattatt taagtgatac tccattaggg ccgagtgctc acaacgctcg aaaggtgttt
     2041 ggaatggttg aattcaaggc cgttgttaac gacatgaaga ccggcaggtc ctacagcgtc
     2101 ccggtttccg ggcatcacgc caattccctg atcgggaaga agatcgggga caccatcgat
     2161 ggcatattcg tagggctgcc tggctacaag ctgacgatca ctggcggaag cgacaaggac
     2221 ggcttcgcca tgagg
//
LOCUS       DAOSYN010000226         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_37236_length_2228_cov_3.522780, whole genome shotgun sequence.
ACCESSION   DAOSYN010000226 DAOSYN010000000
VERSION     DAOSYN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37236_length_2228_cov_3.522780"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..716)
                     /locus_tag="PLJ11_07295"
     CDS             complement(381..716)
                     /locus_tag="PLJ11_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04506.1"
                     /translation="MTKLFDELRREPILLAYHPLCGRYDDHFIEIGGRKVCRGCVTIY
                     PVALGVFLALAWVRPGFMPSFVLSLALFAVQLLRFASSRGRMRTVLNVLLGSSLAAAS
                     VPPSPALPR"
     gene            842..1954
                     /locus_tag="PLJ11_07300"
     CDS             842..1954
                     /locus_tag="PLJ11_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO04507.1"
                     /translation="MNRKGIVAVLLVIALVAVGTIVVLGMNPSDEGEEGPVTVIDAAG
                     RAVTTSEAPKRIVSCSPSLTEIVYALGIGDRLVAVTDYCDWPEDVVARKANGSLVSIG
                     GYFTPSGEAVIAAEGDLILIDKGVQAQMDMVSQLEASEQRYIVLEKGTTFDEIYAAIS
                     MIGEICWAEEEAEALIDSMKGRLAAIEDALGDAEDIPSLVFAVWLDPVYLSGNGTFTQ
                     DVILHAQGENVYSNMTGWPEVSIESLLVQDPEYLLVSMMQLPTPGEEIISELENDTMW
                     AEVSAVRNGNVYIFTGQADNVFCRPGPRMIDAVELMAKILHPEVFDMALPHVIGNDYT
                     DWVTSAPVGEAVTGSEVNSPALIGLIQARTDQVPLP"
BASE COUNT          493 a          634 c          697 g          404 t
ORIGIN      
        1 gttccagcag ctgcttccct gcggaagggg tcgctgatag gacctgacac ttccatagta
       61 gggcggtacg tgctgcggcc tttcctcatt tcatgaggtg gaaggtcggg gcagcgggcc
      121 aacagaacaa tggcaggaag ggaacgatca gccaacttcc agtgacctcc ttgctcaagg
      181 cgctcccgcg ctaaggtgtc caatgccgat gagcgatcgc tatccgtttc caattacttc
      241 ccatcctcta gctccataga ctccctagcg catctgggga agacggcgca acaggggcat
      301 ccttagcacc gggaaagcgc ccgccttccc ttgagatact caaagctgat gatcaccgcg
      361 gtgatgaagg gatagagtcc tcagcgtgga agggcaggcg atggcggaac agatgccgcg
      421 gccaagctgc tccccaagag gacgttgaga acggtcctca tcctgccacg ggaggaggcg
      481 aacctcagga gctgcaccgc gaacagagcg agggagagga cgaaggaggg catgaatcca
      541 ggtcgcaccc acgcaagcgc taggaacacc cccagggcca cagggtagat ggtgacgcag
      601 cccctgcaga ccttccgccc tccgatctca atgaaatgat cgtcgtacct gccgcaaaga
      661 ggatggtagg ccaataggat cggctccctc cggagctcat cgaagagctt ggtcacccat
      721 caaccaccat agggtatagc ggcccctcac ggataaaagg ccactgccaa gggctttata
      781 tttggatgat atatccaatc tagttggata atacatccaa taatgctgaa agggatacac
      841 catgaacaga aagggaatcg tcgctgtatt actggtcata gccctggtgg ccgtagggac
      901 catagtcgtt ctgggaatga acccctccga tgaaggggag gaggggccgg tgaccgtgat
      961 cgatgccgct ggaagggctg tcactacctc tgaggcgccg aagcgcatcg tgtcctgttc
     1021 cccctccttg accgagatcg tgtacgcgtt gggcatcggc gaccggctgg tggccgtgac
     1081 ggactactgc gattggccgg aggacgtggt cgcaaggaag gccaacggct ccttggtctc
     1141 gatcggaggg tatttcactc cgagcggaga ggcggtcatc gccgctgaag gggacctcat
     1201 attgatcgat aagggcgtac aggcccagat ggatatggtc tcccagttgg aggcctccga
     1261 acagaggtac atagtgctcg agaaggggac caccttcgac gagatctatg cggccataag
     1321 tatgatcggg gagatatgct gggccgagga ggaggccgag gccctcatcg attccatgaa
     1381 ggggcgcctc gccgctatcg aggacgcgct gggggatgcc gaggacatcc cctccctggt
     1441 ctttgccgtc tggctcgacc ccgtctatct gagcggcaac ggcaccttca cccaggacgt
     1501 gatcctccac gcccaggggg agaacgtcta ctccaacatg accggatggc cagaggtcag
     1561 tatcgagtca ttgctcgttc aggacccgga atacctgctg gtctccatga tgcaactgcc
     1621 aactccaggg gaggagatca tctccgagct ggaaaacgat acaatgtggg ctgaggtctc
     1681 cgcggtgagg aacggcaacg tctacatctt caccggccag gccgacaacg tgttctgccg
     1741 ccccgggccc cggatgatcg atgccgtgga actgatggcc aagatattgc atcccgaggt
     1801 gttcgacatg gccctgccgc acgtgatcgg gaacgactac accgactggg tgaccagcgc
     1861 cccggtcggt gaggcggtca ccgggtccga ggtgaacagc cctgccctca tagggctgat
     1921 acaggccagg acagaccagg tcccattgcc gtgatgaggg cgcagatata ataatggccg
     1981 cgtgctatgg gaagcaaccc cgcccgaggc tgacgcgata cgccagcctc ggggtaaagg
     2041 atccgaggga cagatgatgg acggaaccaa acatgaccgg gcaaagttca agctggcgct
     2101 catccttggg ctagggtcgg tcagcctggt cgtgctcttc atcctctccc tcaccaccgg
     2161 ggtgatggac atctcgttcg gggaggccat gacgggcgtg tggaacctca tacggaacgg
     2221 cggggtgc
//
LOCUS       DAOSYN010000227         2224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_37321_length_2224_cov_3.426003, whole genome shotgun sequence.
ACCESSION   DAOSYN010000227 DAOSYN010000000
VERSION     DAOSYN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37321_length_2224_cov_3.426003"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="PLJ11_07305"
     CDS             complement(<1..1207)
                     /locus_tag="PLJ11_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04508.1"
                     /translation="MPDIEFRLTATDEASPVIDKARKKMDQFNAQQTSASRKSSAAYG
                     RMGGGTKESGGMSGIIGKGVIGNLAGLAIIAKAGLDIMKKAWAVLNQASPYLQSVMSQ
                     FKTAVNIYLRPLGDAIAKMLAPRSEYAIKKAEERSEAITQLEDDYGAIGALLGTFGFG
                     LVDAYSAIAEFKYGMMNAMSKIAMWPLKKLGDLIGIKIPGSLSELQESIFGIEGGFQG
                     LKEWFEGLPDKLSKRLNEAWENIKSGLSNAWSNLSNLGSDIWNGITGTLNNAWSVLSN
                     FGSWLWTKITDAFSGAWSIISNFGTWLWEKITNAFGGAWSIISNFGNWIWTKITDALS
                     NIGASISGFGTWLWNKITGALSDITTKISGFGTWLWTKITDSLSTGWETLKTIGTTIY
                     YSIKNATSGI"
     gene            complement(1289..1675)
                     /locus_tag="PLJ11_07310"
     CDS             complement(1289..1675)
                     /locus_tag="PLJ11_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04509.1"
                     /translation="MSEQIEFDPVRLAEPRIKTIHVEGVGDVKYSMLTLEDYLALKEL
                     PNETEKDTYIMGLEMAHRMLVKAYPELTIDHMLHWPHEELTKITEALLDIPDFRARPS
                     ENGSGSQTRPKHSGRSAGSSKSSPPK"
     gene            complement(1685..2101)
                     /locus_tag="PLJ11_07315"
     CDS             complement(1685..2101)
                     /locus_tag="PLJ11_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04510.1"
                     /translation="MAKYVIKTPQITVNGVDLSSYLEDVTLTYEADEIESTSSGTTVN
                     DKSFVAGLRSWSVDATLHQDYAASQVDATLFPLVGADPFTILLKPTSAAVGAGNPSFS
                     GSAILLSYPPMTGKVGELAKVTIKFRGTGALTRATS"
BASE COUNT          482 a          637 c          476 g          629 t
ORIGIN      
        1 caatccctga ggtagcattc ttgatcgaat aatatatggt ggtaccgatc gtctttaatg
       61 tttcccatcc tgtagataac gaatctgtta tcttggtcca taaccaggtt ccgaagccgg
      121 atatcttggt tgtaatatca ctaagagcgc cagttatttt attccatagc caggttccga
      181 acccggatat actagctccg atattgctta aggcatcggt tatcttggtc catatccaat
      241 ttccgaaatt agatatgatc gaccatgctc ctccaaaggc attggttatc ttttcccata
      301 gccatgtccc aaagttagag ataatggacc aggcaccgct aaaggcatct gttatcttag
      361 tccataacca cgagccaaag ttcgataata cgctccaggc attattgaga gttccagtta
      421 ttccattcca gatgtccgag ccgagattag atagattgct ccaagcattg cttaatccac
      481 ttttgatatt ctcccaagcc tcatttaatc gtttgctaag cttgtcaggc aatccctcga
      541 accactcttt cagtccctga aatccacctt ctatgccaaa gatcgactct tgaagttcac
      601 taagagaacc tggtatcttg atcccaataa gatcgccgag cttcttcaat ggccacatgg
      661 caatcttgct cattgcattc atcatgccat atttgaactc ggctatagct gaataggcat
      721 cgacaagccc aaatccaaat gttccgagca atgctccgat ggctccgtaa tcgtcctcta
      781 attgagtaat ggcttcagac cgttcttcag cctttttgat ggcatactca gaccttggag
      841 cgagcatctt cgcgatcgca tccccaagcg gcctgagata gatatttacg gcggtcttaa
      901 actggctcat cactgactgg agatatggcg atgcttgatt caggacagcc caggccttct
      961 tcattatgtc caatccagcc ttggcgatta tggccaatcc tgctaagttt cctattaccc
     1021 cttttcctat tatgccagac attcctcctg attctttagt tccgccgccc atcctgccat
     1081 aggccgccga gctctttctg gatgcggacg tctgctgagc attgaattgg tccatcttct
     1141 tgcgggcctt gtcgatgact gggctggcct cgtccgttgc cgtcagccta aattcaatgt
     1201 ctggcattct ctatggcctc tttctccaat gtcctatgcc tttccagccc gctcagcatg
     1261 aatgcatact catcgaaccc gcatttcatc atttcggagg ggagcttttt gaactcccag
     1321 cagatcgtcc agaatgcttg ggcctcgtct gagagccaga cccattttct gacggcctcg
     1381 cccgaaaatc cggtatgtcc agcaatgcct ccgtgatctt cgtcagttct tcatgcggcc
     1441 aatggagcat atgatcgata gtcagctcag gatatgcctt taccagcatc ctgtgcgcca
     1501 tctccagccc cattatgtat gtgtccttct cggtctcgtt tggaagctcc ttgagcgcta
     1561 ggtagtcctc gagggtgagc attgaatatt tcacatcgcc caccccttcg acatggatgg
     1621 tctttattct gggctcggcc aatcgaacag gatcgaactc tatctgttcg ctcattcgat
     1681 caccttagct cgtcgcccgc gttagtgctc cagtcccacg gaacttgatc gtcaccttcg
     1741 ccaactctcc caccttcccg gtcatgggag gataggacaa caggatcgcg gacccggaga
     1801 aactgggatt gccagccccg acggccgcag acgtcggctt gagcaggatc gtgaatggat
     1861 cggcccccac caatgggaac agagtcgcgt ccacttggct tgccgcatag tcctgatgca
     1921 atgttgcgtc cacgctccac gatcgaagtc ctgctacgaa gctcttgtcg ttcacggtcg
     1981 tgcccgagct ggtgctctct atctcgtccg cctcgtaggt caaggtcacg tcctccaaat
     2041 agcttgagag gtccactcca ttgaccgtta tctgaggcgt cttgatcaca tatttcgcca
     2101 tttttcctca ctccttagat tattcccaat cctacatcga atgttgcgct cggacttgcg
     2161 ccgctaatgg tccaatccac ccggtaccac gtgtcggtta tcgctccggc tttgctcttg
     2221 acct
//
LOCUS       DAOSYN010000228         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_37442_length_2218_cov_3.470643, whole genome shotgun sequence.
ACCESSION   DAOSYN010000228 DAOSYN010000000
VERSION     DAOSYN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37442_length_2218_cov_3.470643"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /gene="csx17"
                     /locus_tag="PLJ11_07320"
     CDS             <1..2055
                     /gene="csx17"
                     /locus_tag="PLJ11_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csx17"
                     /protein_id="HOO04511.1"
                     /translation="EEEVRSSVGPSFERLREVGRGAGALLEEMERLGVPKGKRSLYQE
                     FLVALNIRDELQASGRWDEVKNMPIEAASSRKDPSLSALLPKVKKIRTHIDGKARKGM
                     KEVIVRKCRSTLPEGCIQWIDAAMILDADGKTKCPAVLGGSGGNEGNLDYSRTFIKNV
                     TDLLLSSDEGRCRELLANSLFDERTDKLPISPMGMFDPGRAGGFNQGVGIELKDFPSN
                     PWDFVLTLEGSILWASSITRREGDRAGTMSSPFTTRSKGVGYSSASELDAGSDAYEVW
                     APVWGSPATLAEVRYLMAEGRAYIGKAQPQHSIQFAIALANLSVDRGIREFVRYGILK
                     RRGDSKVMMPLGRFEVRKVKYAYLLEELDAILAPIDRMLARFVGEPPASLRVARRKVD
                     VAILNVVAYDSVASYRALLQAIGGLEEVLALRDPEAEPRLARPPGGMSLEWLLACDDG
                     TPEVRMAGAISSLYDKGLGPWRAHMAPIDPKRPYSWAKDRGMLSWEGGDAYRRMAGSL
                     HRRIIVAVREGNAHPLRSRLQLHPADVMPLVRGEVDERALCELLWGMNWLNWWDLDRS
                     APVLRQVRSQWSRPLGIEMVPRGWTMLAAALSPDPIIEEGDDRKGITPDASLVSLLMA
                     NRYQEAVAAARRKLFSQGVPTRQAVPREPLVGMNVAASLLVPVHLRPVLRSIVKRNEG
                     DE"
BASE COUNT          432 a          666 c          751 g          369 t
ORIGIN      
        1 gaggaggagg tccggtccag cgtcgggccc tccttcgagc ggttgcggga ggtcggccga
       61 ggggcgggcg cattgttgga ggagatggag aggctcgggg ttccgaaggg caaacgcagc
      121 ctttaccagg aattcttggt cgccctcaac atcagggacg agctgcaggc ctctggcagg
      181 tgggacgagg ttaagaacat gccgatcgag gccgcttcga gccgcaagga tcccagcctc
      241 tccgcccttc tcccgaaggt gaagaagata cggacccaca tcgatggcaa ggcaaggaag
      301 gggatgaagg aggtcatcgt gaggaaatgc cgctccacat tgcccgaggg atgcatccag
      361 tggatcgatg cagcgatgat cctggatgcc gatggcaaga ccaagtgccc cgcggtcctg
      421 gggggcagcg gcgggaacga agggaacctg gattactcca ggacgttcat caagaacgtt
      481 accgacctcc tgctatcctc ggacgaaggg agatgcaggg agctgctggc caattccctt
      541 ttcgacgagc gtaccgacaa gctcccgata tcccccatgg gcatgttcga ccccggtcgg
      601 gccgggggct tcaaccaggg ggtggggatc gagctcaagg atttcccatc gaacccctgg
      661 gacttcgtgc tcacgctcga gggcagcatc ctctgggcaa gctcaataac ccgcagggag
      721 ggcgatcgcg ccggcaccat gtcctcgccg ttcaccacaa ggtccaaggg ggtggggtac
      781 tcctccgcct cggagctcga cgctggaagc gatgcgtatg aggtctgggc ccccgtgtgg
      841 ggaagcccag ccacgctcgc cgaggtcagg tacctgatgg ccgaggggcg ggcgtacata
      901 gggaaggccc aaccccagca cagcattcag ttcgccatcg cccttgccaa tctgagcgtg
      961 gaccgcggga tcagggagtt cgttcggtac ggcatcctaa agcgccgcgg ggacagcaag
     1021 gtaatgatgc cgctcgggcg gttcgaggtg aggaaggtca aatacgccta cctcctggag
     1081 gaattggacg ccatcctcgc ccctatcgat cggatgctcg cccggttcgt cggtgaaccg
     1141 cccgcctccc tccgggtcgc ccgacggaaa gtggatgtcg cgatattgaa tgtggtggcg
     1201 tacgactcgg tggcctccta tcgcgccctc ctccaagcga tcggagggct cgaggaggtc
     1261 ctggcgctca gggaccccga ggccgagccc cggctagcgc gtcccccggg agggatgagc
     1321 ctcgaatggc tgctggcctg cgatgatggc acccccgagg tgcggatggc gggggccatc
     1381 agctccctct atgacaaagg gctcggcccc tggagggcgc acatggcccc gatcgatccg
     1441 aagaggccct attcctgggc gaaggacagg gggatgctca gctgggaagg gggggacgcc
     1501 tatcgcagaa tggcggggtc cctgcatcgc cgcatcatcg tcgcggtcag agaggggaac
     1561 gcccaccccc tccgctccag gctccagctt catcctgccg atgtgatgcc gttggtcaga
     1621 ggggaggtgg atgagagggc cctatgcgaa ctcctatggg gaatgaattg gctcaactgg
     1681 tgggacctgg accgatccgc ccccgtcctc agacaggtac gctcccaatg gtcccgcccc
     1741 ctgggcattg agatggtccc acggggatgg accatgctcg ccgcagccct ttccccagac
     1801 ccgatcatcg aggagggcga tgaccgcaag gggatcacac ccgacgcatc cctggtctcc
     1861 ctgctgatgg ccaacagata ccaggaggcg gtggccgccg cccgtcgcaa gctgttctct
     1921 cagggcgtcc caacgcgtca ggccgttccc cgggagccct tggtgggcat gaacgtcgcg
     1981 gcatccctgc tggtgccggt gcatttgagg ccagtgctaa gatcgatagt caaaaggaac
     2041 gaaggagatg aatgaatgcc gaattggttg caggagtgca ggaacaagaa caggatgata
     2101 atcgaggccg atctggcgcc cgtggtaggc gaccggttcc aaccaactgg tttcgcggac
     2161 ctcggcgcag ccatatacga acgcccggac gggacgagga tgctcctcgt ggagagcg
//
LOCUS       DAOSYN010000229         2212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_37578_length_2212_cov_3.455726, whole genome shotgun sequence.
ACCESSION   DAOSYN010000229 DAOSYN010000000
VERSION     DAOSYN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37578_length_2212_cov_3.455726"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLJ11_07325"
     CDS             <1..917
                     /locus_tag="PLJ11_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO04512.1"
                     /translation="VPPEDLVVGARVALNKQTLAVMSVLPQSLDPIVTGAEIIEKPTT
                     TYDMIGGLDEQILEVREAVEDPLLRPELYKRVGIEPPKGVLLVGPPGTGKTLIAKAVA
                     NHTNATFIRFVGSELVQKYIGEGARLVRELFELAREKAPSIIFIDELDSVGAKRLDVA
                     TSGDREVQRTLMQLLAELDGFNPLDNIKIIGATNRPDILDEALLRPGRFDRIIEVPIP
                     NYEARLEIFRIHTARMSVEEGLNLETLAMKTELATGADIKSICTEAGMFAIRDNRDVV
                     SMADFERAVIKVLANETSKCTESGVMFA"
     gene            complement(897..1070)
                     /locus_tag="PLJ11_07330"
     CDS             complement(897..1070)
                     /locus_tag="PLJ11_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04513.1"
                     /translation="MKAWVLTCPDCGSADVIYEAGMVLGQKYRCLKCGYMGSLALERE
                     IEVSEKGSGEHDA"
     gene            complement(1101..1898)
                     /locus_tag="PLJ11_07335"
     CDS             complement(1101..1898)
                     /locus_tag="PLJ11_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOO04514.1"
                     /translation="MIRTTAFLSGDTMRFIYFVGTAGSGKSSLIYAFQEWMTLQGLDS
                     VIINLDPGVEHMPYEPDIDIRDWVRVDDVMEEYGLGPNGAQIVAADMMALNAKDLAES
                     MEQFRTNYVLVDTPGQIELFAFRQSSEVIMEELGKGDSCLVFLADPNLCKSPSGFVSA
                     IMLAATVHFRFSIPFFTVLSKSDLLKEEELEQITEWSESTDALYAALTGKIDPRAVLG
                     IELFKALEAIGAYRRVVPASAVEPSGLEDIYDMVQESFEGGEDLSDD"
     gene            complement(1935..>2212)
                     /locus_tag="PLJ11_07340"
     CDS             complement(1935..>2212)
                     /locus_tag="PLJ11_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04515.1"
                     /translation="QSVGGSVEDLRKRLGRMARKLLQGGIAGISRAEPQNLRERMGRL
                     DDPPTRSYPMAMKLTGDRGGRKAASASTRRRADKVEGLLKALERLTR"
BASE COUNT          395 a          742 c          679 g          396 t
ORIGIN      
        1 acgtcccgcc agaggacctg gtggtggggg cccgggtcgc ccttaacaag cagaccctgg
       61 ccgtgatgtc ggtcctgccc cagtccctcg accccatcgt caccggggcg gagatcatcg
      121 agaagccgac caccacctac gacatgatcg gggggctcga cgagcagatc ctggaggtgc
      181 gcgaggcggt ggaggacccc ctgctccgac cggaacttta caagcgcgtg ggcatcgagc
      241 cccccaaggg cgtactgctg gtcggtccgc cgggcacggg gaagacgctc atcgccaagg
      301 cggtggccaa tcacactaac gccaccttca tccgcttcgt cggctcggag ctggtgcaga
      361 agtacatcgg agagggggcc aggcttgtcc gtgagctctt cgagctcgcc cgcgagaagg
      421 ccccgagcat cattttcatc gatgagctgg actcggtcgg cgccaagcgc ctggacgtcg
      481 ccacgtccgg agaccgggag gtccagcgca ccctcatgca gctgctcgcg gagcttgatg
      541 gcttcaatcc cttggacaat atcaagatca tcggggccac caaccgtccg gatatcttgg
      601 acgaggctct gctccgtccc ggccgcttcg accgcataat cgaggtgccc atcccgaact
      661 acgaggcccg gctggagatc ttccgcatcc acacggcccg catgagcgtg gaggaggggc
      721 tgaacctgga gacactggcg atgaagaccg agctggccac cggcgcggac atcaagtcca
      781 tatgcacgga ggcgggcatg ttcgccatcc gcgacaatcg ggacgtggtg agcatggccg
      841 acttcgagag ggctgtcatc aaggtcctgg cgaacgagac ctccaagtgc accgagtcag
      901 gcgtcatgtt cgcctgagcc cttctccgag acctctatct ccctctccag cgcaagggag
      961 cccatgtacc cgcacttcaa gcagcggtac ttctggccca gcaccatccc cgcctcatat
     1021 atcacgtccg cggagccgca gtccgggcag gtgagcaccc aggccttcat gccctaagca
     1081 ctcgctaccg ggaagataaa tcaatcgtcg gaaaggtcct ccccgccctc gaaggactcc
     1141 tgcaccatgt cgtagatgtc ctccaggccg gacggctcca cggccgaggc gggcacgacc
     1201 ctccgatagg cgccgatggc ctccaaggcc ttgaagagct cgatcccgag gaccgcccgg
     1261 ggatcgatct tgccggtgag cgcggcatag agagcgtcgg tggactcgga ccattccgtg
     1321 atctgttcca gctcctcctc cttgagcagg tcgctcttgg acagcacggt gaagaaaggg
     1381 atgctgaagc ggaagtgcac ggtggccgcc agcattatcg ccgagacgaa ccccgagggg
     1441 gacttgcaca ggttggggtc ggccaggaac accaggcagg agtccccctt gcccagctcc
     1501 tccatgatga cctcgctgct ctgccggaag gcgaagagct cgatctggcc gggggtgtcc
     1561 accaggacgt agttcgtgcg gaactgctcc attgattcgg ccaggtcctt ggcgttgagg
     1621 gccatcatgt ccgccgccac gatctgcgcg ccattggggc cgaggccgta ctcctccatg
     1681 acgtcgtcca cccgcaccca gtcccggatg tcgatgtccg gctcgtaggg catgtgctcc
     1741 actccggggt ccaggttgat gatgacggag tccaggccct gcagggtcat ccattcctgg
     1801 aaggcgtaga tcagggagct cttgccgctc ccggcggtac ctacgaagta gatgaagcgc
     1861 attgtatcgc ccgaaaggaa cgccgtcgtc cttatcatac ttgcgccgcg cctcggaaga
     1921 aaggccaagg gcgttcacct cgtcagcctt tctaatgcct tgagcagccc ctccaccttg
     1981 tccgcccttc tcctggtcga ggcgctggcc gccttcctcc cgcccctgtc cccggtcagc
     2041 ttcatggcca tcgggtagga gcgggtggga gggtcgtcta ggcgccccat cctttcccgg
     2101 aggttctgcg gctcggccct ggagatgccg gcgatccctc cttgcaggag cttcctggcc
     2161 atccttccca ggcgtttccg gaggtcctcc acagatcccc ccacgctctg gt
//
LOCUS       DAOSYN010000230         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_37812_length_2201_cov_2.732060, whole genome shotgun sequence.
ACCESSION   DAOSYN010000230 DAOSYN010000000
VERSION     DAOSYN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37812_length_2201_cov_2.732060"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLJ11_07345"
     CDS             <1..231
                     /locus_tag="PLJ11_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04516.1"
                     /translation="PESDRDITLEVELPHEAETYLNANIAKSAITAITLPIGNKTLTL
                     SNGRLIGGNSPELKQALLSETLRFQFKTLSIA"
     gene            240..845
                     /locus_tag="PLJ11_07350"
     CDS             240..845
                     /locus_tag="PLJ11_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04517.1"
                     /translation="MDLIEINSGDLPGDMRRIALAPLLEQHRKRWMLRTCAGDVLLKR
                     ITALEAEQVTLDAMADPDFVGMVERAHVLSAKAAGPGLEGDELREYLEVGARLAPFAK
                     RIALKCIVSPEVRSDEEYDVLLNALSTDERRALEDLLRQLSRTDLDGEVTTSALALSE
                     KYGIPLAPDLTVENMTAQQSAALSQRVSAENDRLAAMLRRK"
     gene            847..1380
                     /locus_tag="PLJ11_07355"
     CDS             847..1380
                     /locus_tag="PLJ11_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04518.1"
                     /translation="MPAEDWYYDVTEGMRENIVALDRYNATSGSKSELEQGDLGASLM
                     ASQAAYRSINGGLQRGLASAAALGLFTDENNEVMQGLYKALTATSAAFAAYQVIRGMV
                     AAKTALEASMAAAEVTLHTLALDFVGIAVAAGAAGAAYGMTQYVQGEWTFPQPDISTV
                     SGRHQAADAVVRASTGA"
     gene            1382..1969
                     /locus_tag="PLJ11_07360"
     CDS             1382..1969
                     /locus_tag="PLJ11_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04519.1"
                     /translation="MAAGMDTSINWTFNMVDNVSATTKQMADSQAQLAGNASAAKASI
                     DSQNISFIAQTTAVMATYGGVRRLTSSLTELGLVSEGDAVKLQKLNATLGLVAGGFQL
                     MKGATQIIKMLTQAEIGLAAVESFRAVLNNPAKIALVGAGLGLAAGVGAALLATSGGG
                     GGGGTTVNQTITFEGAATQDQRSVARETYEAMGGF"
     gene            1969..>2201
                     /locus_tag="PLJ11_07365"
     CDS             1969..>2201
                     /locus_tag="PLJ11_07365"
                     /inference="COORDINATES: protein motif:HMM:NF021665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HOO04520.1"
                     /translation="MAEIHSAGTGNWHTPGTWTGGVVPGTGDTAVVDAGHTVTIAADV
                     NLIYKIIVYGNLVMNADITMDNSNGCYIWVTDNG"
BASE COUNT          454 a          642 c          767 g          338 t
ORIGIN      
        1 ccggagagcg accgggacat aacgctggag gtcgagcttc cccatgaggc ggaaacgtac
       61 ctgaacgcca acatcgccaa gagcgccatc accgccatca ccctcccgat cggcaacaag
      121 acgctgacgc tgagcaatgg gcggctgatc ggcgggaact cccccgagct gaagcaggcg
      181 ctgctaagcg agacgctgag gttccagttc aagaccctga gcatcgcctg aggtggaaca
      241 tggacctcat cgaaataaac agcggggacc tgcctggaga catgcggagg atcgccctcg
      301 cacctctact ggagcagcac cggaagcggt ggatgctgcg gacctgcgcc ggggacgtcc
      361 tgctcaagcg cataaccgcc ctggaggccg agcaggtcac gctcgacgcc atggccgacc
      421 cggacttcgt gggcatggtg gagagggcgc acgtcctgag cgccaaggcc gccggtccgg
      481 ggctggaggg tgatgagctg agggaatacc tggaggtagg cgctaggctc gcccccttcg
      541 ccaagcgcat cgccctgaaa tgcatcgtca gtccggaggt gcggtccgat gaggaatacg
      601 acgtcctgct gaacgctctg tccacggacg aacggagagc gctggaggac ctgctcaggc
      661 agctctcgcg cacggacctg gacggggagg tgaccacgtc ggcgctggcg ctgtccgaga
      721 agtacggcat accgctggct ccggacctca cggtggagaa catgaccgcg cagcagtcgg
      781 cggcgctgtc gcagcgggtg agcgccgaga acgataggct ggccgcgatg ctcaggagga
      841 agtgagttgc ccgccgagga ctggtactac gatgtgacgg aggggatgcg cgagaacatc
      901 gtggctctgg accgctacaa cgccacgtcc ggatctaagt ccgaactgga gcagggagac
      961 ctgggcgctt cgttgatggc gtctcaggca gcgtaccggt ccatcaatgg agggctgcag
     1021 aggggtctcg cttcggccgc agctcttggc ttgttcaccg atgagaacaa cgaggtcatg
     1081 caggggctgt acaaggcatt gacggccacc agcgcggcct ttgccgcgta tcaggtcatc
     1141 aggggcatgg tggcggccaa gacggccctg gaggcatcca tggccgccgc ggaggtcact
     1201 ctgcacacac tggcgctgga cttcgtcggg atcgccgtgg ccgccggtgc agccggggcg
     1261 gcctacggga tgacgcagta tgtccagggc gaatggacct tcccgcagcc ggacatatcc
     1321 accgtttccg gcaggcatca ggcggcggac gcggtggtcc gcgcatcaac gggggcatga
     1381 catggcggca gggatggaca ccagcattaa ttggaccttc aacatggtgg acaacgtgag
     1441 cgccaccacc aagcagatgg ccgactcgca ggcgcagctg gccgggaacg cgtcagcggc
     1501 caaagcatcc atcgactcgc agaacatctc gttcatagcg cagaccaccg cggtcatggc
     1561 gacgtacggc ggagttagac gcttgacctc atcgctcacc gaactggggc tggtcagcga
     1621 aggggacgcc gtgaagctcc agaagctcaa cgccacgctg gggctggtgg ctggcggctt
     1681 ccagctgatg aagggcgcga cgcagataat caagatgctc acgcaggccg agatcgggct
     1741 ggcggcggtg gagtcgttcc gcgccgtgct gaacaatccg gccaagatcg cattggtagg
     1801 cgcgggtctg ggacttgcgg ctggcgtggg cgcggctctc ttggccacca gcggaggcgg
     1861 cgggggcggt ggaacgacgg taaatcagac gataaccttc gagggagcgg ccacccagga
     1921 ccagcgcagc gtggcgcgtg aaacgtacga agcaatggga gggttctgat ggcagagatc
     1981 catagcgcgg gaacgggcaa ctggcacact ccggggacat ggaccggggg agtggtcccc
     2041 ggaacgggcg atactgcggt cgtagatgct ggtcacaccg ttaccatagc cgccgacgtg
     2101 aatctgatat acaagataat tgtttatggc aatctggtga tgaacgccga catcaccatg
     2161 gacaactcga acgggtgcta tatctgggtc accgataacg g
//
LOCUS       DAOSYN010000231         2170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_38504_length_2170_cov_2.008511, whole genome shotgun sequence.
ACCESSION   DAOSYN010000231 DAOSYN010000000
VERSION     DAOSYN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38504_length_2170_cov_2.008511"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLJ11_07370"
     CDS             complement(<1..410)
                     /locus_tag="PLJ11_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOO04521.1"
                     /translation="MAEKVAHEALEAKIAPLDAIENGLGKGMMTLSDRFDRAEVYLPQ
                     ILAASLAMEKAMEVFKPAMKTGEATSKGKVVIATVEGDIHEIGKNVVAALLVGSGYTV
                     YDLGRDVPIEKFIETAAENDADVVGASALMTTTVL"
     assembly_gap    452..461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(617..1999)
                     /locus_tag="PLJ11_07375"
     CDS             complement(617..1999)
                     /locus_tag="PLJ11_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine methyltransferase corrinoid protein
                     reductive activase"
                     /protein_id="HOO04522.1"
                     /translation="MAVCGNPFQLSLFQNIEIRDLAYAGQNKLKALNVVPPQRDGDIL
                     PAETLGLCIDPTAKVIIPPAVTHEIGADAMAMLWETGTYSAKVPTVIIDYGTNAEMSF
                     IDGGRIITGSAAAGPALEGQEIERGMLASCGAISDINPEGAGWRNYVLDEEFNVVPGD
                     VVDPRTGEVIETGEAHGRARGITGTGVIAAVHLAMEGGLAKPPRIITEEGILRLQDGV
                     ELSSKDLEEAGKAIGAIRAGYMTLAYEAGRNPNDIRVAYMSGASGTYVDARKAQRIGL
                     APFSANELHQVGNTSLAMARDLVRQPGLYERLREFAKTLRASHCMFATSEIFKAIYTI
                     ELSHWCYGMPMGMYNEMLAMSQLSPIDIRSADGKVHKDVIKDIPDLGRMGLCILDEIA
                     VQLRSELPGCKHCLKCVKACPENALVLVREEGEWVATIRSDLCNGTACRRCEQECPEG
                     LIKVKHFHYC"
     gene            1983..>2170
                     /locus_tag="PLJ11_07380"
     CDS             1983..>2170
                     /locus_tag="PLJ11_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO04523.1"
                     /translation="MPQTAISENSLAERIGVSRTPIREALQRLEADGLVRVFRQRSSY
                     VAPLDDQAIRSAYFVRLSL"
BASE COUNT          412 a          704 c          549 g          495 t
ORIGIN      
        1 aggaccgtag tggtcatcag ggcagaggcg cccaccacgt ccgcgtcgtt ctccgctgca
       61 gtctcgatga acttctcgat ggggacatcc cttcctaggt catagaccgt gtaccccgac
      121 cctaccaaaa gggccgcgac cacgttcttt cctatctcat ggatgtctcc ctcgaccgta
      181 gcgatgacaa ccttgccctt ggacgtggcc tcaccggtct tcatggccgg tttgaacact
      241 tccatggcct tctccatcgc caacgaagcg gccaatattt ggggcagata cacttccgcc
      301 cgatcgaatc ggtcgctcaa ggtcatcatt cctttcccaa gcccgttctc tattgcatcc
      361 aacggagcta ttttggcttc gagagcttca tgggcgacct tttcggccaa tttccaatcc
      421 catgtctcga ctgccttctg tagatccgcc annnnnnnnn ngatgtcctt cttggatcta
      481 ggcatgtttt acctccgggt gtatatagtc aatccatata tttataatta acgatatgca
      541 ttatatatac tttacgtaat acttatatag taataacact aaccctttgt aatgatataa
      601 atacttttcc tgtatatcaa caataatgga aatgcttgac cttgatcaat ccctccggac
      661 actcctgttc acatctcctg caggccgtcc cattgcagag gtccgatctg atggtggcga
      721 cccactcccc ctcctcccgt acgaggacca aggcgttctc tggacaggcc ttcacgcatt
      781 tcagacagtg cttgcacccc ggtagctcag accgtagctg gaccgcgatc tcatcaagga
      841 tgcagagccc catcctgcca aggtctggga tgtccttgat cacatccttg tgcaccttcc
      901 cgtcagcgga ccgaatgtcg atcggggaca gctgcgacat cgccagcatc tcattgtaca
      961 tccccatggg catgccatag caccaatggc tcagctcgat cgtgtagatg gccttgaaga
     1021 tctcggaggt ggcgaacatg caatggctcg ccctcagcgt tttggcaaat tccctcagcc
     1081 tctcatagag gcctggctgc ctcaccaggt ccctcgccat ggccaatgag gtgttaccta
     1141 cctgatggag ttcgttcgct gagaacggcg caagcccgat acgctgggcc ttcctggcat
     1201 cgacataggt cccggacgct ccggacatgt aggccacccg aatgtcgtta gggttcctgc
     1261 cagcctcata ggccaaggtc atgtacccag cccgaatggc cccgatggcc ttgcccgcct
     1321 cctccaggtc cttcgaggaa agctcgacgc catcctgcag cctcaagatc ccctcctctg
     1381 tgattatcct ggggggcttc gccaggccgc cttccatggc caaatgcacg gcggcgatca
     1441 cccccgtgcc cgttatgccc ctcgcccttc cgtgggcctc cccggtttca atgacctccc
     1501 cggtgcgcgg atcgacgaca tctcccggca cgacattgaa ctcctcgtca agcacgtagt
     1561 tcctccagcc agcgccttca ggatttatgt cggagatcgc cccgcaggag gcgagcatgc
     1621 ccctttcgat ctcctgtccc tccaaggcag gccctgccgc cgccgagccc gtgatgatcc
     1681 gcccgccatc tatgaaggac atctcggcgt tcgttccata gtctatgatc acggtgggca
     1741 cctttgcgga ataggttcct gtctcccaca acattgccat ggcatccgcg ccaatctcat
     1801 gcgtcaccgc cggagggatg atgaccttgg ctgtggggtc gatgcacagc ccgagggtct
     1861 ccgccggaag gatgtcccca tcccgctgtg gggggaccac gttcagcgcc ttgagcttgt
     1921 tctgacctgc ataggccagg tcccggatct cgatgttctg gaaaagcgag agctggaacg
     1981 gattgccgca gaccgccatc agcgagaact cgctggcgga gcggatcgga gtcagccgaa
     2041 cgccgatccg cgaggcgctg cagcggctcg aggccgacgg gctcgtccgg gtgttccggc
     2101 agcgctcgag ctatgtcgcg ccgctcgacg accaggcgat ccgctcggcc tatttcgtcc
     2161 gcctgtcgct
//
LOCUS       DAOSYN010000232         2158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_38760_length_2158_cov_4.145506, whole genome shotgun sequence.
ACCESSION   DAOSYN010000232 DAOSYN010000000
VERSION     DAOSYN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38760_length_2158_cov_4.145506"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLJ11_07385"
     CDS             <1..209
                     /locus_tag="PLJ11_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOO04524.1"
                     /translation="IAGKGIANPCATILSACLMLTFLRRMDASARLQAALVQTLMEGE
                     RTRDLGGSLGTSEFTDRVAERLVR"
     gene            249..1049
                     /locus_tag="PLJ11_07390"
     CDS             249..1049
                     /locus_tag="PLJ11_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO04525.1"
                     /translation="MGADLGRTILDRCREWGVRMVGFAAADAWDEPPFEPWPPIEFHP
                     KAIFPGCRTVIVLGLPVTLPILETSPSIWYQELYKNLNAQLDERAYRLSQLLNEFGHA
                     SVYVPRDGYGTVELLVESPYAFFSHRHAAYLAGLGTFGINNMLLTREYGPRVRFVSIL
                     TTAELPPGGPMAKGLCVRCMRCAKACPVQAIGDVAYPEASIDKRRCAARSVELKKRYR
                     SPCGICIKVCPVGEDRKLFGHGNMTYDEEGTPPELWRAWEHVRRYGSL"
     gene            1116..1346
                     /locus_tag="PLJ11_07395"
     CDS             1116..1346
                     /locus_tag="PLJ11_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO04526.1"
                     /translation="MSAKRGPSRPIIDESECKGCGRCVAACPKQVLRLSGRTNRRGYN
                     AVEYAGEGCTGCFACFYNCPEPYALQVDDGRE"
     gene            1349..>2158
                     /gene="vorB"
                     /locus_tag="PLJ11_07400"
     CDS             1349..>2158
                     /gene="vorB"
                     /locus_tag="PLJ11_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOO04527.1"
                     /translation="MRRFVKGNEAVAIGAIYAGCDVYFGYPITPASDVAHAAAEYFPQ
                     LGREFLQAECETGTINMMFGAASAGKLAMTASSGPGISLMQEGISYMAGAQLPAVIVN
                     VQRVGPGLGNIGPEQGDYNQAVKGGGHGNYHCLVLAPSSVQEMCDLTMKAFELAFKYR
                     NPAIVLADAVLGQMMEFLELPEREMPRPDTASWAVQGDAVTRGNLVSSIYLDSDLQEK
                     HNWALQGKYETMEQDAMAECYLTEDAEIVLTGYGTSSRVARSAVDELRGLGV"
BASE COUNT          385 a          642 c          754 g          377 t
ORIGIN      
        1 acatagccgg gaaagggata gcgaacccct gcgcaaccat cctttcagca tgcctgatgc
       61 tcacgttcct gaggaggatg gacgcgtccg cccggctcca ggccgccctg gtgcagaccc
      121 tgatggaggg ggaacgcacc cgggacctgg gagggagcct cggcacctcg gagttcacgg
      181 acagggtggc ggagaggctt gttcggtgaa gggcaattat ttttattgcc ccctccaatc
      241 catccgccat gggcgcggac ctggggcgta ctattttgga caggtgccgg gagtgggggg
      301 tgcggatggt ggggttcgcc gcggcggacg cctgggacga gccgcccttc gagccctggc
      361 cgcctattga gttccatccc aaggcgatct tccccggctg ccgcacggtg atcgtgctgg
      421 ggcttccggt caccctgccc atcctcgaga cttcgccctc gatctggtac caggagctct
      481 acaagaacct caacgcccag ctggatgaga gggcgtatcg gctctcccag ctcctgaacg
      541 agttcgggca cgcctctgtc tacgtcccgc gggacgggta tgggacggtg gagctgctcg
      601 tggagagccc gtacgccttc ttctcccacc ggcacgcagc ctacctggcc ggcctgggga
      661 ccttcggcat caacaatatg ctattgacca gggaatacgg gccgcgggtg cgcttcgtct
      721 ccatcctcac caccgccgag ctgccccccg gcggcccgat ggcgaagggg ctctgcgtgc
      781 ggtgcatgcg ctgcgcaaag gcctgcccgg tgcaggcgat cggggacgtc gcgtatccgg
      841 aggcctccat tgacaagcgc aggtgcgccg ccaggagcgt ggagctcaag aagaggtacc
      901 ggtcgccctg cggcatttgc atcaaggtct gcccggtggg cgaggaccgc aagctgttcg
      961 ggcatgggaa tatgacgtat gacgaggaag ggacgccccc cgagctttgg agggcgtggg
     1021 agcatgtgcg caggtacggc agcctttgat gggatgcgac cacggcgaag ctcattgaag
     1081 taggtttaag taccgcccat ccatccagag gggggatgag cgcaaagagg gggccgtccc
     1141 gtccgatcat cgatgagagc gagtgcaagg gctgcggccg atgcgtggcc gcctgcccca
     1201 agcaggtatt gcgcctctcg ggccgcacga accgccgagg atacaatgcc gtcgagtacg
     1261 ctggcgaggg gtgcacgggc tgcttcgcgt gcttctacaa ctgcccggag ccgtacgccc
     1321 tgcaggtgga tgacgggagg gagtgaccat gcgcaggttc gtcaagggca acgaggcggt
     1381 ggccatcggc gccatctacg ccgggtgcga cgtttacttc ggctatccga tcacgccggc
     1441 gagcgatgtt gcgcacgccg cggcggagta cttcccgcag ttgggaaggg agttcctcca
     1501 ggcggagtgt gagaccggga ccatcaacat gatgttcgga gcggcctccg ccgggaagtt
     1561 ggccatgacc gcctcctcgg gccccggcat ctccctgatg caggagggca tatcgtacat
     1621 ggcaggggcg cagctgcctg ctgtcatagt aaatgtgcag cgtgtcgggc cagggcttgg
     1681 caatatcggg ccggagcagg gggactacaa tcaggcggtg aaggggggcg ggcatggcaa
     1741 ttatcactgc ctagtgcttg cgccctcctc cgtacaggag atgtgcgacc tgaccatgaa
     1801 ggcgttcgag ctggccttca agtaccgcaa tccggccata gtgcttgcgg acgcggtcct
     1861 cggccagatg atggagttcc tagagcttcc ggagagggag atgccccggc cggacacggc
     1921 ctcctgggcc gtacaagggg atgcggtgac ccgtggcaat ctcgtctcgt cgatatacct
     1981 ggacagcgac ctgcaggaga agcataactg ggcccttcag gggaagtacg agaccatgga
     2041 gcaggacgcc atggccgaat gctatctcac cgaggatgcg gagatcgtcc tgacaggata
     2101 tggtacgagc tcgcgggtgg cccgctcggc cgtggacgag ctcaggggcc tgggcgtt
//
LOCUS       DAOSYN010000233         2154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_38853_length_2154_cov_3.531205, whole genome shotgun sequence.
ACCESSION   DAOSYN010000233 DAOSYN010000000
VERSION     DAOSYN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38853_length_2154_cov_3.531205"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1630)
                     /locus_tag="PLJ11_07405"
     CDS             complement(113..1630)
                     /locus_tag="PLJ11_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO04528.1"
                     /translation="MRLTFYGGAGTIGGNKVHVELGGYGAFLDFGEPFDIGAMYCSEY
                     VRPRASRGLRDFLRLGMLPPLDCYRPDLCPADLPASEMRAVRVDALFLSHAHSDHYGH
                     AGFLRHDIPIVASPMTYAILRASSEIGRGGPPTELFDHTVRELKEDEPRVLVTPRTKG
                     RQVHGREWVLTGRAIEDAPEWRKGGSCMVREGEGELNGIEYRAYPVDHSVYGATAFAL
                     REGDGEWLVYSGDLRMHGTNGGSTAMFADEARRLAPKALIIEGTRLGRPSSGVEMTEA
                     MVRENCLAAVEASDGLVIADFSANNFERLDTFFDIARRTGRRMVVTHRDVAFLESVGR
                     VDGNVRKDLLWVYRSLGRDDVEEVGRLGREGWQVADPDDIANAPDEHMLCLSFWDMPK
                     LLDLDRRGGTYIYSNSMAYDLRRTDDLQKLMNWTRLLGFECRGLAVVDGTLGFEEGFH
                     ASGHLAPEDLWRVISTISPEVVVPVHCACPELFAELCPVPTVLPEKGRPIDLGSD"
     gene            complement(1930..>2154)
                     /locus_tag="PLJ11_07410"
     CDS             complement(1930..>2154)
                     /locus_tag="PLJ11_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04529.1"
                     /translation="ALAGVSLMVGIAVWFYRHPDGDQGRTGTQTMGPSTPYEMEKGHN
                     MVKQNELNRIGLSGYWVDRRYFGRFKGPKD"
BASE COUNT          396 a          762 c          612 g          384 t
ORIGIN      
        1 ccgggccaag cagggcaggg tggtccttgt gggctcgata ggcgtccccc tcctgtcatc
       61 caccgaagac caaggaccgt cggccgaacg aaggccccct ccgacctaag gatcaatccg
      121 aacccaggtc gatcggcctt cccttctccg gcaggacggt cggcacaggg cacagctctg
      181 cgaacagctc cgggcaggcg cagtgcaccg ggaccaccac ctcgggcgag atcgtgctga
      241 tgaccctcca gaggtcctcc ggggcgagat ggccggaggc atggaaaccc tcctcgaacc
      301 ctagcgtccc atccaccacg gccagccccc ggcactcgaa ccccagaagc ctggtccagt
      361 tcatcagctt ctgcaggtcg tccgtccgcc tcaggtcgta ggccatgctg ttggagtaga
      421 tgtacgtgcc ccccctccgg tccaggtcaa gcagcttggg catgtcccaa aaggagaggc
      481 agagcatatg ctcgtcggga gcgttggcga tatcgtcagg gtccgccact tgccacccct
      541 cccttccgag ccgccccacc tcctcgacgt catcccggcc cagggagcgg tagacccaga
      601 gcaggtcctt gcggacgttg ccgtcgaccc tccctacgct ctccaggaag gccacgtccc
      661 tatgggtgac gaccatccgc ctgccggtcc tgcgggcgat gtcgaagaat gtgtccaagc
      721 gctcgaagtt attggccgag aaatcggcga tgacgagccc atcgctcgcc tccacggccg
      781 ccaggcagtt ctcccgtacc atcgcctcgg tcatctcgac gccggacgac ggtctgccga
      841 gccgggtgcc ctcgatgatc aacgccttgg gggcaagcct cctcgcctca tccgcgaaca
      901 tggcggtgga ccctccattg gtcccgtgca tccgcaggtc cccggaatag acaagccatt
      961 ccccatcccc ctcccggagg gcgaaggccg tcgccccgta cacggaatgg tcgaccggat
     1021 acgccctgta ctcgatgccg ttcagctctc cctcgccctc ccggaccatg caggaaccgc
     1081 ccttcctcca ttcaggggca tcctctatgg ccctgccggt aagaacccac tccctcccgt
     1141 gcacctgcct gcccttggtc cgaggggtca caagcacccg gggctcgtcc tccttcagct
     1201 cccttaccgt atggtcgaag agttccgtcg gcggaccgcc cctgccgatc tcgctggacg
     1261 ccctcaatat ggcatacgtc ataggactgg cgacgattgg gatgtcatgc ctgaggaacc
     1321 ccgcgtggcc atagtggtcc gagtgggcat gtgagaggaa cagggcgtcg acccgcactg
     1381 ccctcatctc cgatgccggg aggtcggcgg ggcacaggtc cgggcgatag cagtccaggg
     1441 gcggaagcat gcctaaccgc aggaagtccc tcagcccccg gctggcccgc ggcctgacgt
     1501 actcggagca gtacattgca ccgatatcga acggctctcc gaagtccagg aaggctccgt
     1561 agcctcccag ctcgacgtgc accttgttac cgccgatcgt ccccgccccg ccgtagaacg
     1621 tgagccgcat gtcctcgccc ttcaaaggcc atgccaggat ttatcgatat ccacatcctt
     1681 ggcgaacccc gtcatccaac agaaagggac gtaaatggca tctgacggca tatcatgagc
     1741 ccggggggcc ctgtccccca tcgggcattg cccgtggaga aatgggaagc gcgaacgcct
     1801 tgcataagga cgatgggccg gccaacgttg cacgggcaat gggcccgtcc ggacgaacac
     1861 gatatcaaga tcctctcgcg gtcatatcca tcgacgatgg cgatcgagcg accgttccgg
     1921 gattcgggat caatccttgg gacccttgaa ccttccgaaa taccttctgt ccacccagta
     1981 cccgctcagg cctatccggt ttagctcgtt ctgcttgacc atattgtgtc ctttctccat
     2041 ctcgtatgga gtgctggggc ccatggtctg tgtcccggtg cggccctgat caccatccgg
     2101 atgccgatag aaccagaccg caatcccgac catcaggctc acaccagcca gggc
//
LOCUS       DAOSYN010000234         2152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_38903_length_2152_cov_3.564616, whole genome shotgun sequence.
ACCESSION   DAOSYN010000234 DAOSYN010000000
VERSION     DAOSYN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38903_length_2152_cov_3.564616"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLJ11_07415"
     CDS             complement(<1..310)
                     /locus_tag="PLJ11_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HOO04530.1"
                     /translation="MELGLGIDTGGTYTDAALVELGTGKVIAKAKALTTRNDLSIGIA
                     GSLRNLGDVPLKDVKLVSLSSTLATNSVVEGKGCRVALIIAGDAYNGSVPVDHIIQVQ
                     G"
     gene            complement(377..1558)
                     /locus_tag="PLJ11_07420"
     CDS             complement(377..1558)
                     /locus_tag="PLJ11_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04531.1"
                     /translation="MAVLLECQGVSYDYPGRGAALRDVHLEIVKGRKTAIIGPNGAGK
                     STLFLHLNGVLRPKGGQVRFQGEPIVYRNKDLSLLRKEVAVILQNPDDQIFSATVEED
                     VAFGPLNLGLPKEEIEARVDEALALVDMTGERERPSQQLSFGQKKRLALAGALAMRPK
                     VLIMDEPTAGLDPHMVQEVLELTEELHMKGITLVMSTHELEVAYSWADDFVVMNLGKV
                     VFSGPAEGLFANRSLLELIGFQAPDVYRMNEEMHRIGLFELTPVPKHMTDLRMKFCRM
                     NGRAMGGMSIRRVDKGELPSITEVHTLISQGRAVGYYGSRAKHLLATMLPSDVRLPGL
                     RGCLDKMVDGRDAMLYTDGMLVPAIMDHINNLAQRYGLAINVMDGWTEEGHAADGWPT
                     G"
     gene            complement(1562..>2152)
                     /gene="cbiQ"
                     /locus_tag="PLJ11_07425"
     CDS             complement(1562..>2152)
                     /gene="cbiQ"
                     /locus_tag="PLJ11_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOO04532.1"
                     /translation="ALVLEGIGIMVLGGAVIAAVTPGETVGALDLALLKITFSGEGIT
                     TASMVTLRALAGISVMLFFATSTPIPHFADMLVRFKVPKLFAELMVMVYRYSFMLLDE
                     VGRMHLAAECRLGFHGRMNTLRTYAKMMTGMFIRSIETSERSNIGMQCRNYQGGVGML
                     RQPQGMSAPWAVLCLAVFLALLELNLFCERCGILLG"
BASE COUNT          419 a          710 c          587 g          436 t
ORIGIN      
        1 cgccctggac ctgtatgata tggtccacgg gaacgctccc attataggcg tcgccagcta
       61 tgatgagggc gaccctgcac cccttgccct ccaccacgga gttggtggcc agggtggatg
      121 acagggaaac gagcttgaca tccttgaggg gcacatcccc caagttcctc agggagcccg
      181 cgatcccgat gctcaggtcg ttcctggtcg tcagggcctt ggccttggcg atgacctttc
      241 cagtcccaag ctccaccaat gccgcgtccg tgtatgtgcc tccagtgtcg atccccaatc
      301 ccagttccat aggctgcctc caagcgtagg ccttgaatta attttttaaa gccatccagc
      361 ccttataaac aaatcgttag ccggtgggcc atccatcggc agcatgccct tcctcggtcc
      421 atccatccat cacattgatg gctaggccgt acctctgggc caaattattt atatggtcca
      481 tgatagcggg caccagcatt ccatccgtat agagcatggc atccctgccg tccaccatct
      541 tgtccaagca gcccctcagc cctgggagcc ggacatccga aggcaacatg gtggccaaca
      601 gatgcttggc ccgggagccg tagtatccga cggcccttcc ctgggagatg agggtgtgca
      661 cctcggtgat cgatggcagc tcacccttgt ccaccctcct gatcgacata ccccccatcg
      721 ctcgtccatt catccggcag aatttcatcc tgagatcggt catgtgcttt ggcacgggcg
      781 taagttcgaa caggccaatg cgatgcatct cctcgttcat ccgatagacg tctggagcct
      841 ggaacccgat gagctccagc agggagcggt tggcgaagag cccttccgcg gggcctgaga
      901 agaccacctt ccccaggttc atgaccacaa agtcatcggc ccaggaatag gccacctcca
      961 gctcatgggt cgacatcacg agcgtgatcc ctttcatgtg cagctcctcc gtcagctcca
     1021 ggacctcctg caccatgtgt gggtccagcc ctgccgtcgg ctcatccatg atcaggacct
     1081 tggggcgcat ggccagcgcc cctgccaagg ccaggcgctt cttctgcccg aaggacagct
     1141 gctgggacgg tcgctccctc tccccggtca tatcgaccaa agctaatgct tcgtccaccc
     1201 tggcctcgat ctcctccttt ggcagtccca gattcaatgg gccgaaggcc acgtcctcct
     1261 ccaccgtggc ggagaatatc tgatcgtccg ggttctgaag gatcaccgcc acctccttgc
     1321 ggagaaggct gaggtccttg ttcctgtaca ctatcggctc gccttggaac cgcacctgcc
     1381 cgcccttcgg cctcaggacg ccgttgagat gcaggaacag cgtggacttg ccggcgccgt
     1441 tagggccgat gatcgcagtc ttcctgccct tcactatctc caagtgcacg tcccgcaggg
     1501 cggcccctcg cccggggtaa tcgtagctta ccccttggca ctcgaggagg acggccatgg
     1561 ttcaccccag caatatgccg catcgttcgc agaacaggtt cagctccagg agcgccagga
     1621 aaacagcgag gcagagcacg gcccagggag cgctcatccc ctggggctgc cggagcattc
     1681 ccactccgcc ctggtagtta cggcattgca tgccgatgtt ggacctttcc gatgtctcta
     1741 tggaccggat gaacatcccc gtcatcatct tggcataggt gcggagggtg ttcatccgac
     1801 cgtggaaacc gaggcggcat tcagcggcca gatgcatccg ccccacctcg tccagcagca
     1861 tgaaggaata cctgtagacc atgaccatca gctccgcgaa gagcttgggc accttgaagc
     1921 ggacgagcat gtcggcgaaa tgggggatgg gcgttgaggt ggcaaagaaa agcatgacgc
     1981 tgatccccgc gagggcccga agggtcacca tcgaggcggt ggttatgcct tccccagaga
     2041 atgttatctt gaggagcgca aggtctagcg ccccaaccgt ctccccgggc gtgaccgccg
     2101 cgatcaccgc ccctcccagg accattatcc ctatcccttc aaggaccagt gc
//
LOCUS       DAOSYN010000235         2104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_39992_length_2104_cov_3.735481, whole genome shotgun sequence.
ACCESSION   DAOSYN010000235 DAOSYN010000000
VERSION     DAOSYN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39992_length_2104_cov_3.735481"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLJ11_07430"
     CDS             complement(<1..455)
                     /locus_tag="PLJ11_07430"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOO04533.1"
                     /translation="MVDFRPFRPFIPRLGSGERMLDRVSPPYDVISAEELGRLRANRF
                     NVTNITLGGVDGDYSAAGLALSSWIEQGALAQDRRDAFYTYRQTFCEGEHYWQRTGIV
                     GIMAAEGYSERVVPHEETFPKVKEDRLNLLRGTGTHCESIFGIFDELSPG"
     gene            complement(493..1608)
                     /locus_tag="PLJ11_07435"
     CDS             complement(493..1608)
                     /locus_tag="PLJ11_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO04534.1"
                     /translation="MADPKASKVYFTDMRTCQGNDLLHKMERLLRRAGLDRMDLDGKL
                     VAIKLHMGEPGNLAYVRPSLAAVLVRMVKEKGGRPFLTDCNTLYMGRRANAVDHLNSA
                     MENGFNRIAVGCDVIIADGLRGNEHREVLIEKELCRTAKIGSAIADADVVLSLTHFKG
                     HDLTGFGGTLKNLGMGSGSRCGKMEMHASSKPTVDRRKCVGCGLCARSCPEGAIAIDE
                     KRKAVIDHGKCVGCGQCVAVCQHGAPEVEWEGSSEIVNKKIAEYAYAVLHDKPHFHVS
                     VLMDISPNCDCWTFNDVPIVPDIGFAASRDPVALDMACARLVNEAEAERGSVLGTRGT
                     EGDKFKAVHPATDWMTGLEHAERIGLGKRAYELVRLK"
     gene            complement(1690..>2104)
                     /locus_tag="PLJ11_07440"
     CDS             complement(1690..>2104)
                     /locus_tag="PLJ11_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOO04535.1"
                     /translation="GDDTDGHLDDFARFVSRSTVLCARAEEGEDAPVLRRNWERLRQY
                     KDRFGLEVQALPMPRPIFLEEEERNLPASYANFYIGNRVVLAPVFGDPADRAALETLS
                     SHFPGREVVPIPARELVYGYGGVHCVTQQEPAEPD"
BASE COUNT          329 a          753 c          630 g          392 t
ORIGIN      
        1 ccgggggaga gctcgtcgaa gatcccgaaa atggattcgc agtgcgtccc cgtcccgcgc
       61 aaaaggttga ggcgatcctc cttgaccttg gggaaagttt cctcgtgcgg gaccaccctc
      121 tcggaatagc cctccgcggc catgatgccg acgatccccg tccgctgcca gtaatgctcg
      181 ccctcgcaga atgtctgccg gtaggtgtag aacgcgtccc tgcggtcctg ggccagcgcg
      241 ccctgctcta tccaggagga gagcgcgagg ccggcggcgg agtagtcgcc gtccactcct
      301 cccagcgtga tgttggtcac gttgaagcgg ttcgccctca gcctaccaag ctcctccgcc
      361 gagatcacgt cgtagggcgg ggagacgcgg tcgagcatcc gctcgccgct gccaaggcgg
      421 gggatgaacg gccggaaggg acggaaatcg accatgggcc tggaacgcgc tagccgctat
      481 tagcttttgc aatcacttga gccggaccag ctcatacgcc ctcttcccga gcccgatgcg
      541 ctccgcatgt tccagcccgg tcatccagtc cgtggcgggg tgcacggcct tgaacttgtc
      601 gccctccgtc cccctggtcc ccagcacgct ccccctctcc gcctccgcct cgttcaccag
      661 ccgggcgcag gccatgtcca gggccaccgg gtccctggag gcggcgaagc cgatgtccgg
      721 gacgatgggc acgtcattga aggtccagca gtcacagttc ggggagatgt ccatgagcac
      781 gctgacgtgg aagtggggct tgtcgtgcag caccgcgtag gcgtattcgg cgatcttctt
      841 gttgacgatc tccgacgacc cctcccattc cacctccggg gcgccatgct ggcagaccgc
      901 cacgcattgg ccgcagccca cgcatttgcc gtggtcgatg accgccttcc gcttctcgtc
      961 gatggcgatc gccccctcgg ggcagctcct ggcgcagagc ccgcagccca cgcacttcct
     1021 gcggtccacc gtgggcttgg aggaggcgtg catctccatc ttgccgcatc tgctcccgct
     1081 ccccatcccc aggttcttca gcgtcccccc gaagccggtc aggtcgtgcc ccttgaagtg
     1141 ggtgagggag agcacgacat cggcgtcggc tatggcgctc ccgatcttgg ccgtgcggca
     1201 cagctccttc tctatcagca cctcccggtg ctcgttcccc ctgagcccat ccgcgatgat
     1261 gacgtcgcac cccaccgcga tgcgattgaa gccgttctcc atggcgctgt tcaggtggtc
     1321 caccgcgttc gccctcctcc ccatgtagag cgtgttgcag tcggtgagga acggccttcc
     1381 ccccttctcc ttgaccatcc tcaccagcac cgcggccagg ctggggcgga cgtaggccag
     1441 gttgcccggc tcccccatgt gcagcttgat cgccaccagc ttgccgtcca aatccatgcg
     1501 gtccaggcct gccctgcgca gcaatctctc catcttatgc agcagatcgt tgccctgaca
     1561 ggtgcgcatg tccgtgaagt agaccttgga tgccttggga tcggccatgg cattgaatcg
     1621 gcctcggcgc tattaattat gtgccatccg aaccgtccca ggcccccggg gcgcccttgc
     1681 cgatcagcct caatcaggct ccgccggctc ctgctgggtc acgcaatgga cccccccgta
     1741 cccgtagacc agctcccggg ccggaatggg cacgacctcc ctccccggga aatgggagga
     1801 gagagtctcc agggccgcgc ggtcggccgg gtccccgaac accggggcaa ggaccaccct
     1861 gttgcctatg tagaaattgg catagctcgc tggcaggttc cgttcctcct cctccagaaa
     1921 gatgggccgg ggcattggaa gggcctggac ctccaggccg aagcggtcct tgtactgcct
     1981 caagcgctcc cagttcctcc tcagcaccgg ggcgtcctcc ccctcctcgg cccgggcgca
     2041 cagcaccgtg gagcgggaga cgaagcgggc gaaatcgtct aagtgcccgt cagtgtcgtc
     2101 cccc
//
LOCUS       DAOSYN010000236         2100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_40081_length_2100_cov_4.082152, whole genome shotgun sequence.
ACCESSION   DAOSYN010000236 DAOSYN010000000
VERSION     DAOSYN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40081_length_2100_cov_4.082152"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLJ11_07445"
     CDS             <1..228
                     /locus_tag="PLJ11_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04536.1"
                     /translation="SNKEGLKFCSYQVGYNILVGARHDSNKEGLKFCSYCGTAYNIEA
                     MVRVIQHALELDSAHPGRWGRMHHDYIQEQV"
     gene            255..596
                     /gene="eif1A"
                     /locus_tag="PLJ11_07450"
     CDS             255..596
                     /gene="eif1A"
                     /locus_tag="PLJ11_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HOO04537.1"
                     /translation="MSNEPYTQGDQGAESEEILRCPYPNKKEGEMFGIADQLLGASRI
                     KIMCADGKSRMGRIPGKIKKRMWIREGDLVIIRPWDFQEDKCDVLYRYTRTQASHLSR
                     KKALPKNLDIF"
     gene            604..1395
                     /locus_tag="PLJ11_07455"
     CDS             604..1395
                     /locus_tag="PLJ11_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HOO04538.1"
                     /translation="MPFEDKRILKLDREVEGLRLTRSYERSPEGRKVTEEVFDRLTLE
                     VISKLIREGSIAKLDFPVSTGKEGNVFRATAPDGSFVALKIFRVSNSTFKNIARYIEG
                     DPRFSGITGSTRKLIYAWTTKEFKNLCRLEAARVCVPHPVRFLRNVMVMEYIGDEAMA
                     APQLRSVRLEKPQKVYREVVRFMRRAYQRAGLVHGDLSEYNILMHEGRPYIIDCGQAM
                     MADHPNAQGLLERDIRNINRYFSGLGVRVWGDGTVLRYVKGVRER"
     gene            1392..1937
                     /locus_tag="PLJ11_07460"
     CDS             1392..1937
                     /locus_tag="PLJ11_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOO04539.1"
                     /translation="MKVVKVPKERVGVLIGQGGETKRYLEERTGLRIDVDAEEGEVSF
                     DEMKAEDPLLPLKVIDIVRAIGRGFAPQKAFRLLEDDEYLEIMNIDNYVGKGSNQLAR
                     MRARVIGSGGKTRRIIEDLVGVHVSVYGDTVSIIGNSVQMPVARTAVDMLLSGSEHAT
                     VYRYLERKRAELRIAEMGFEL"
BASE COUNT          452 a          599 c          687 g          362 t
ORIGIN      
        1 tcaaataagg aagggctcaa gttctgttcc taccaggtcg gctataatat cctggtgggt
       61 gcgcgtcatg actcaaataa ggaagggctc aagttctgtt cctactgcgg cacggcgtac
      121 aatatcgagg ccatggtcag ggtcatccag catgcgttgg agttagacag cgcccacccg
      181 ggacgatggg gccggatgca ccatgattat atacaggaac aggtttaatg gaatcctatc
      241 gttggaggat tccattgagc aacgaacctt acacccaggg ggaccaaggc gccgagagcg
      301 aggagatcct tcgctgcccg tatccgaaca agaaggaggg cgagatgttc gggatagcgg
      361 accagctgct gggtgcctcc cgcatcaaga tcatgtgcgc tgatggcaag tcccgaatgg
      421 gtcgcatccc aggcaagatc aagaagagga tgtggatccg agagggggac ctggtcatca
      481 tccgcccctg ggactttcag gaggacaagt gcgacgtcct ctatcgctat accaggacgc
      541 aagcgagcca tctgagccgc aagaaggcct tgcccaagaa ccttgacatc ttctgagggg
      601 cctatgccgt tcgaggacaa gcggatcctc aagctggaca gggaggtgga ggggctccgc
      661 ctcacccgtt cctatgagcg ctcgcccgag gggcgcaagg tcaccgagga ggtgttcgac
      721 cggctcacgc tggaggtgat ctccaagctc atccgcgagg gaagcatcgc caagctggac
      781 ttcccggtgt cgaccggaaa ggaggggaac gtgttccggg ccaccgcccc cgacgggtcc
      841 ttcgtcgccc tgaagatatt ccgcgtctcc aattccacgt tcaagaatat agcgcgctac
      901 atagagggcg acccccgctt ctccggcatc actgggagca cccggaagct catatacgcc
      961 tggacgacga aggagttcaa gaacctctgc cgcctggagg cggcgagggt ctgcgtcccg
     1021 caccccgtcc gcttcctgag gaacgtaatg gtcatggagt acatagggga cgaggccatg
     1081 gccgccccgc agctgcgctc ggtccgactt gagaagcccc agaaagtgta ccgcgaggtc
     1141 gtgcgcttca tgaggcgcgc gtaccagagg gcggggttgg tacacggcga cctgagcgag
     1201 tacaacatcc taatgcacga aggtcggcct tatatcatag actgcggaca ggccatgatg
     1261 gccgaccacc ccaacgccca ggggctgctc gagcgggaca tccggaacat caaccgctac
     1321 ttcagcgggc tgggggtcag ggtgtggggc gatggcaccg tcctcagata tgtaaagggg
     1381 gtcagggagc gatgaaggtt gtgaaggtcc cgaaggagcg cgttggcgtg ctcatcggtc
     1441 agggcgggga gaccaagagg tatctggaag agcggacggg cctcaggatc gatgtggacg
     1501 ctgaggaggg ggaggtctcc tttgacgaga tgaaggcgga ggacccgctg ttgcccctca
     1561 aggtcatcga tatcgtgagg gcgatcggcc ggggattcgc cccgcagaag gcgtttcggc
     1621 ttctggagga cgacgagtac ctggagataa tgaacatcga caactatgtg ggcaagggca
     1681 gcaaccagct cgcccgcatg cgcgccaggg tcatcggctc cggcgggaag acccgccgca
     1741 taatcgagga cctggtcggc gtgcacgtct ccgtctacgg ggacaccgtc agcatcatcg
     1801 gcaactcggt gcagatgccc gtcgcgcgca ccgcggtcga catgctgctc agcgggagcg
     1861 agcatgccac ggtgtatcgc tacctggaaa ggaaacgggc ggagctgcgc atcgccgaga
     1921 tggggttcga gctctaatac ctaatcgccg ttcctcagga gggccatcag gttccggggg
     1981 aacagctgca ggtccagccc caacggtgcc aagcgctcgg ccatccgcac ggccctcggg
     2041 ccaacgaaat cgcccttcag gaggtactcc gtgtcctcct cgtcgaactc ggtgcaatgc
//
LOCUS       DAOSYN010000237         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_40302_length_2090_cov_3.823096, whole genome shotgun sequence.
ACCESSION   DAOSYN010000237 DAOSYN010000000
VERSION     DAOSYN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40302_length_2090_cov_3.823096"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLJ11_07465"
     CDS             complement(<1..482)
                     /locus_tag="PLJ11_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOO04540.1"
                     /translation="MTLRLYESDPYLKEFDATVTKVEGEWVVLDRTAFYPGGGGQERD
                     QGTLAGLPVLGMRGKDEIAHRVPGHAFTVGDKVHGTLDWVNRYALMRAHTGEHLLFSS
                     LSRRTDMELVKISLARERKVLVVKGHLDWGIVREAVQEVNSIIAQGAKVSSHLVDRES
                     "
     gene            626..1090
                     /locus_tag="PLJ11_07470"
     CDS             626..1090
                     /locus_tag="PLJ11_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOO04541.1"
                     /translation="MLDELDKRIIEELCRSSQGSSRAIAKRLGIHPTTLIQRVRNLES
                     RNIIRGYRANVDYMKLGYEFMAIVHIYVEGDLMEVQRRITELKNVMAVLDVTGECDSI
                     AWVACRSREEFSQVVKSMLTIKGVKKTNTYVILNVIKDPFNFLPDFSPIEQK"
     gene            1264..>2090
                     /locus_tag="PLJ11_07475"
     CDS             1264..>2090
                     /locus_tag="PLJ11_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOO04542.1"
                     /translation="MLRTHHCGQLTKGDLGKRVTLAGWVRFYRDHGGVLFYDIADSHG
                     STQAVFDPEATTSGDLDRLEGVLNSIGREYVVAVTGTVRDRVEGTTDPRNPTGEIEVL
                     IEDAEVLNRSKPIPFEVAEQKNSMLPGEDLRMRYRYLDLRRKEMNANLRFRSRLISSA
                     RRFLESEGFMEVETPMLTRSSREGARDFLVPSRTMPGHFYALPQSPQLYKQMLMVGGV
                     ERYFQIARCFRDEDSRADRQPEFTQLDLEMSFVDMDDILSMVERMYAFVWKELFNEP"
BASE COUNT          416 a          627 c          640 g          407 t
ORIGIN      
        1 atgctctccc ggtccacgag atggctgctg accttcgctc cctgggcgat gatggagttg
       61 acctcctgca ccgcctcccg cacgatcccc cagtccagat ggcccttgac gaccaggacc
      121 ttcctttccc gggccaggga gatcttgacc aactccatgt cggtgcgtct ggagagggag
      181 gagaagagca ggtgctcgcc ggtgtgggcc ctcatcaggg cgtagcggtt cacccagtcc
      241 aaggtcccgt ggaccttgtc cccgacggtg aaggcgtgcc ctggtacgcg gtgcgctatc
      301 tcgtccttcc ccctcattcc gaggacggga agccctgcca acgttccctg gtccctttcc
      361 tgccccccgc cccccgggta gaaggcggtg cgatcgagca cgacccattc cccttccacc
      421 ttggtcactg tggcatcgaa ctccttcagg taggggtcgc tctcgtacag cctcagggtc
      481 atgggcggct gattggtgtt ccctatgcat aaagtcctcg ttaagtcttg gaaggaagtg
      541 ttagacaatt gcgcactcta ttcccgtctt aaaagacata tatctaagga aggcattgta
      601 gaggcgggac cacggggcgt tcatcatgct tgacgaattg gacaagagga taatcgagga
      661 gctgtgcagg tccagccagg ggtcctccag ggccattgcc aagaggctgg gcatccatcc
      721 caccaccctg atccaaaggg tgcgcaacct ggagagccgc aatatcatcc gcggctaccg
      781 ggccaacgtc gactatatga agctgggcta tgagttcatg gccatagtgc atatctatgt
      841 cgagggggac ctcatggagg tgcagcggag gatcacggag ctgaagaacg tcatggccgt
      901 cctggacgtc acgggcgagt gcgactccat cgcctgggtg gcctgccgct cccgcgagga
      961 gttcagccag gtggtgaagt ccatgctcac cataaagggg gtcaagaaga ccaataccta
     1021 tgtcatcctg aatgtgatca aggacccctt caacttcctg ccggacttca gcccgatcga
     1081 gcagaaatga gggctgcaga ggagcgttcc gtccatagcg cctctcatcc cttggcagtt
     1141 tggacctgtt gaaccgatta cattgccatt cgggaggctc ggccaggtca gtagtggata
     1201 agggtttaat agggcgtcca cgttactccg gcctaccgac catatccaac cagaggaatg
     1261 gagatgttga ggacacacca ttgcggacaa ctgacgaagg gggaccttgg caagagagtg
     1321 accctcgctg gttgggtaag gttctacagg gaccatgggg gggtgctgtt ctacgatata
     1381 gcggactccc acgggagcac ccaagcggtg ttcgaccctg aggccaccac cagcggggac
     1441 ttggaccggt tggagggtgt cctcaatagc atcggccgcg agtacgtcgt cgccgtcacc
     1501 ggcaccgtgc gggacagggt ggaggggacg accgatccca ggaacccgac cggggagatc
     1561 gaggtgctca tcgaggacgc ggaggtcctc aaccgctcga agcccatccc gttcgaggtg
     1621 gccgagcaga agaactccat gctgcccggc gaggacctca ggatgcggta tcgctacctg
     1681 gacctcaggc gcaaggagat gaacgccaac ctgaggttcc gttcgaggct catctcctcc
     1741 gcccgccgct tcctggagtc cgagggcttc atggaggtgg agaccccgat gttgacccgc
     1801 agcagccggg agggcgccag ggacttcctg gtcccctccc ggaccatgcc agggcatttc
     1861 tacgctctcc cccagtcccc ccagctctac aagcagatgc tgatggtcgg tggcgtcgag
     1921 cgctacttcc agatcgcccg atgcttccgc gacgaggaca gcagggcgga ccgtcagccc
     1981 gagttcacgc agctcgacct ggagatgtcc ttcgtggata tggacgacat cttgagcatg
     2041 gtcgagcgca tgtacgcctt cgtctggaag gagcttttca acgagccgtt
//
LOCUS       DAOSYN010000238         2051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_41249_length_2051_cov_3.583166, whole genome shotgun sequence.
ACCESSION   DAOSYN010000238 DAOSYN010000000
VERSION     DAOSYN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41249_length_2051_cov_3.583166"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLJ11_07480"
     CDS             <1..293
                     /locus_tag="PLJ11_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOO04543.1"
                     /translation="AEAQAAFGKAFRAPGHIHLLNSTPHLLSERKGHTELATAMMVMA
                     GVTPTATICEMMGDDGDALSKEKAKRYAARFDLCFLEGKDIIDFWKRGGNGK"
     gene            290..736
                     /locus_tag="PLJ11_07485"
     CDS             290..736
                     /locus_tag="PLJ11_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOO04544.1"
                     /translation="MTRVMASGVFDILHPGHLRYLQEAREQGDELVVVVATDATVRRR
                     KHEPITPERMRLELVSALKMVDHAYLGTDGDMFTVVEHIRPDIIALGYDQDFDERQIE
                     EALRQRGLEVKVVRLSKHGEDLNGTRKIISKIIDWYTTNRSKVEGG"
     gene            736..1200
                     /gene="ribC"
                     /locus_tag="PLJ11_07490"
     CDS             736..1200
                     /gene="ribC"
                     /locus_tag="PLJ11_07490"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOO04545.1"
                     /translation="MRTIGIVDTTFARADMGAAAIDELKRYGTGFNIVRRTVPGIKDL
                     PVAAKVLLDEGCDIVMALGMPGAKPTDKTCAHEASLGIIAAQLMTNKPIVEVFVHEDE
                     AEEGRLAWLMEQRAREHARNAYDMIFKPERLTKNAGRGLRQGYEDVGPIRER"
     gene            1203..1613
                     /gene="ribH"
                     /locus_tag="PLJ11_07495"
     CDS             1203..1613
                     /gene="ribH"
                     /locus_tag="PLJ11_07495"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOO04546.1"
                     /translation="MTRYNIGIVVSEYNYDITSMMLERAKAHAEFLEVNVTKVVHVPG
                     VYDIPLAVKMLLKDNSIDGVVTLGCVIEGETEHDEIVMQNAARKITDLSVEFMKPVAL
                     GITGPGMTRLQAEDRIENAKNAMEACVKMLKRLY"
     gene            complement(1660..>2051)
                     /locus_tag="PLJ11_07500"
     CDS             complement(1660..>2051)
                     /locus_tag="PLJ11_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO04547.1"
                     /translation="ARLRLSANAPCQMAVIEALKHPKDHLEGMRSKLKARGEFAYKRV
                     NEIPKLSCTRPQAAFYMFPRVESDHWKNDRDFVLDILNNCHVLLVPGTGFCPVYGKDH
                     FRMVFLPNEEILGQAFDAIEAYMRKVA"
BASE COUNT          441 a          568 c          709 g          333 t
ORIGIN      
        1 aggcggaggc ccaggccgcc ttcggaaagg cgttccgggc ccccgggcac atccatctgc
       61 tcaattccac gccccatcta ttgagtgagc gcaaggggca tacggagctg gccaccgcca
      121 tgatggtcat ggcgggggtg accccgacgg cgaccatctg cgagatgatg ggggacgacg
      181 gggacgccct cagcaaggaa aaggctaaaa ggtatgcggc gcgctttgac ctttgcttcc
      241 tggagggaaa ggacataatc gacttctgga agagaggcgg gaacgggaaa tgaccagggt
      301 gatggccagc ggagtgttcg acatcctgca tccagggcat ctgcgctatc tgcaggaggc
      361 gagggagcaa ggggacgaac tcgtcgtggt ggtggcgacc gacgcgacgg tgcgccgaag
      421 gaagcatgaa ccgatcacgc cggagaggat gcgcctggag cttgtgtccg cgctcaagat
      481 ggtcgaccac gcctatcttg ggaccgacgg ggacatgttc accgtggtgg agcacatccg
      541 cccggacatc atagccttgg gctacgatca ggactttgac gagcgtcaga tcgaggaggc
      601 ccttcggcag agggggctcg aggtcaaggt cgtccggctg tccaagcatg gcgaggacct
      661 caacggcacg cggaagatca tcagcaagat catagattgg tacacaacca atagatcgaa
      721 ggtcgaggga ggctgatgag gaccatcggg atcgtggaca ccaccttcgc ccgcgcggac
      781 atgggcgccg cggcgatcga cgagctgaag cggtacggaa cggggttcaa tatcgtccgg
      841 cgaacggtcc cggggatcaa ggacctccca gtggcggcca aggtcctgct ggacgagggg
      901 tgcgacatcg tgatggcgct ggggatgcct ggcgccaaac cgacggacaa gacgtgcgcg
      961 cacgaggcat ccctcgggat catcgccgcc cagttgatga cgaacaagcc catcgtcgag
     1021 gtgttcgtgc atgaggacga ggcggaggag ggtaggctgg cctggctcat ggagcagcgg
     1081 gctagggagc atgcccggaa cgcctatgac atgatattca agcccgagcg gctgaccaag
     1141 aacgccgggc gggggcttag gcagggctac gaggacgtcg gcccgatcag ggagagatga
     1201 gcatgacaag gtacaacatt gggatagtgg tctcggagta caactacgac attaccagca
     1261 tgatgctgga gcgggccaag gcgcacgccg agttcctgga ggtgaacgtc accaaggtgg
     1321 tgcacgtgcc tggcgtctac gatatcccgt tggcggtcaa gatgctcctc aaggacaaca
     1381 gcatcgacgg ggtcgtcacc ctggggtgcg tgatagaggg ggagaccgag catgacgaga
     1441 tagtcatgca gaacgccgcg aggaagatca ccgacctttc ggtcgagttc atgaagccgg
     1501 tggccctggg cataaccggc ccgggaatga ccaggctgca ggccgaggac cgcatcgaga
     1561 acgccaagaa tgccatggaa gcgtgcgtca agatgctgaa gcggctctac tgaggccctc
     1621 gacaggaccg ccagatagga gaaggggaac ggggcggcct caggccacct tcctcatgta
     1681 cgcctcgatg gcatcgaacg cctgcccgag gatctcctcg ttgggcagga acaccatgcg
     1741 gaagtggtcc ttgccgtaca cggggcagaa gcctgtgcct ggaacgagca ggacatgaca
     1801 gttgttcaat atgtccagca cgaagtcccg gtcgttcttc caatggtccg actcgacccg
     1861 ggggaacatg tagaaggccg cctgcggcct cgtgcagctg agcttgggga tctcgttcac
     1921 ccgtttgtag gcgaactccc ccctggcctt gagcttgctg cgcatcccct ccaggtggtc
     1981 cttggggtgc ttgagcgcct cgatcaccgc catttggcag ggggcattgg cgctcagccg
     2041 aaggcgcgcc t
//
LOCUS       DAOSYN010000239         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_41408_length_2045_cov_4.528643, whole genome shotgun sequence.
ACCESSION   DAOSYN010000239 DAOSYN010000000
VERSION     DAOSYN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41408_length_2045_cov_4.528643"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLJ11_07505"
     CDS             complement(<1..753)
                     /locus_tag="PLJ11_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO04548.1"
                     /translation="MSASDKVLKVSEAKSKDAGRGIARVDPAVMEALGLNAGDVVQIE
                     GKKKTVALVWPGYNEDANRGVIRIDGTIRRNAQTSIDEKVSIRKVTVKPAQKITFAPT
                     EELRIMGGEEYLAQMMEGRVFTRGDVVELNVMGRRIDLVVMAHSPAADAVLLTRDTEI
                     KLSDKPTKEAPSGIPKVTYEDIGGLGDEVRKVREMIELPLKHPELFERLGVEAPKGVL
                     LHGPPGTGKTLLAKAVAGETSANFVSISGPEIM"
     gene            complement(782..1270)
                     /locus_tag="PLJ11_07510"
     CDS             complement(782..1270)
                     /locus_tag="PLJ11_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOO04549.1"
                     /translation="MAIMKRERKEPVDLSPRAWAWSPVSMFEEMDRLLNDLRSGLVMP
                     GYDVLPMEGARVPTLDLKEEADRFLVQAELPGMTKDDVSIEMDDEVLVITANKEQERE
                     EEEGGYIRRERGCMRFYRQLRLPENVDRGGIKAKMENGVLEVVLPKVKASEEKRSKIE
                     VE"
     gene            complement(1351..1644)
                     /locus_tag="PLJ11_07515"
     CDS             complement(1351..1644)
                     /locus_tag="PLJ11_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04550.1"
                     /translation="MESDLDRYVKEIRRIGGDLERALRAAAQDLDRLGREALTEAAMN
                     AKQGGKEAEQALLRLERELREGGPNIRRGMEELQRRMNEAAAKLEEELKRPSK"
     gene            complement(1625..>2045)
                     /locus_tag="PLJ11_07520"
     CDS             complement(1625..>2045)
                     /locus_tag="PLJ11_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04551.1"
                     /translation="ARRRYFPTMGFTMVVDIGPGLFNAEVATRPFEERATEREGTGRR
                     LMDLRKDIEALDRELEELGQRRAQLIAEKERLLEEAGHLVEEAVPDYRSRRVAYEYIL
                     HPEMDVKELASELGMRDDVVEDIISSLEDDNDGERSG"
BASE COUNT          307 a          761 c          513 g          464 t
ORIGIN      
        1 catgatctcc gggccgctga tggaaacgaa gttggcgctc gtctcccccg ctacggcctt
       61 ggccaagagg gtcttgcccg ttccaggcgg gccgtgcagg agcacgccct tcggcgcctc
      121 gacgcccagg cgctcgaaca gctccggatg cttgagcggg agctcgatca tctcccgaac
      181 cttcctgacc tcatctccca ggcctccgat gtcctcgtag gtgaccttcg gaatgccaga
      241 tggggcctcc ttagtaggct tgtcgctaag cttgatctca gtgtccctgg tcagcagcac
      301 cgcgtccgcc gcgggggaat gcgccatcac caccaggtct atgcgccggc ccatcacgtt
      361 cagctcgacc acgtccccgc gggtgaagac ccgcccctcc atcatctggg ccaggtattc
      421 ctccccgccc atgatgcgga gttcctccgt gggagcgaag gtgatcttct gggccggttt
      481 gacggtcacc ttccggatgg acaccttctc atcgatgctg gtctgggcgt tccgccttat
      541 cgttccgtcg atgcggatca ctccgcggtt ggcgtcctcg ttgtacccag gccataccag
      601 ggccacggtc ttcttcttgc cctcgatctg cacgacgtcc cccgcgttca ggcccaaggc
      661 ctccatgacc gcggggtcca ctctggctat ccccctacca gcgtccttgg atttggcctc
      721 ggacaccttc aataccttgt cggacgcgct catgttatcg ttcctcctta tgaggtggag
      781 atcactccac ctcgatcttg ctcctcttct cctcgctcgc tttcaccttg ggcaggacca
      841 cctctaggac cccgttctcc atcttggcct tgatcccgcc acggtccacg ttctctggca
      901 ggcgcagctg gcggtagaag cgcatgcagc ccctctccct ccggatgtac ccgccttcct
      961 cctcctcgcg ttcctgctcc ttgttcgccg ttatcaccag gacctcatca tccatctcta
     1021 tggacacgtc atccttggtc atcccgggca gctcggcctg caccaggaag cggtcggcct
     1081 cctccttgag gtccaaggta ggcaccctgg ccccctccat tggcaagacg tcatacccag
     1141 gcatgactag cccagagcgc aggtcgttca acagccggtc catctcctcg aacatggaga
     1201 cggggctcca ggcccaagca cggggactaa ggtccaccgg ctctttcctc tctctcttca
     1261 ttattgccat gttgcatcac ctccaacatg ttaactaaga gttgtatact cttagtgcaa
     1321 tactatagtg tttgaacttc tatatatact ttacttagaa ggccgtttaa gctcctcctc
     1381 gagcttggcc gcggcctcgt tcatgcgccg ctgcagctcc tccatccccc ggcggatgtt
     1441 cggcccgccc tccctgagtt ccctctcgag ccgcagtagg gcctgctcgg cctccttccc
     1501 tccctgcttg gcgttcatcg ccgcctcggt gagcgcctcc cgccccaacc ggtccaggtc
     1561 ctgagcggcg gcacgcaggg ccctctccag gtcccccccg atgcgcctaa tctccttcac
     1621 gtatctatcc agatcgctct ccatcgttat cgtcctccaa ggatgagatg atgtcctcga
     1681 ccacatcgtc ccgcatcccc agctcgctcg ccagctcctt gacgtccatc tccgggtgta
     1741 ggatgtactc gtacgccacc ctccggctac ggtagtccgg aacggcctcc tccaccaggt
     1801 gccccgcctc ctccagcagc ctctccttct ccgcgatgag ctgggcccgc ctctgcccaa
     1861 gctcttccag ctcgcggtcg agcgcctcta tgtccttgcg gaggtccatc agcctcctgc
     1921 ccgtcccttc cctctcggtc gcccgctcct cgaagggccg cgtcgccacc tcggcattga
     1981 acaggccggg accgatgtcc accaccatgg tgaagcccat ggtcggaaag taccgtctac
     2041 gggcg
//
LOCUS       DAOSYN010000240         2039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_41569_length_2039_cov_1.874496, whole genome shotgun sequence.
ACCESSION   DAOSYN010000240 DAOSYN010000000
VERSION     DAOSYN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41569_length_2039_cov_1.874496"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLJ11_07525"
     CDS             complement(<1..692)
                     /locus_tag="PLJ11_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04552.1"
                     /translation="MSCEDELTVRVDNVSFSYGTKRNPCGRTLKDITISARKGQLIGI
                     VGPNGCGKTTFMRCINKVLKPQEGAIYIDGKDLDRLRMMEVAKLCANIPADVPDDFHL
                     SVEEFVSLGRYPYVTGIWWEGEKDVRLVREAMEAYHVTHLKDRKLAELSSGEKARVLL
                     AKGAVQQPKILLADEPSAHLDLKFKLQVMQALKDLSRQGVTVITASHDINLIAKYADM
                     VIIISDGGIISYG"
     gene            complement(682..1731)
                     /locus_tag="PLJ11_07530"
     CDS             complement(682..1731)
                     /locus_tag="PLJ11_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO04553.1"
                     /translation="MEGKKAAHPKIKLLMIFILGSISLAFLIILSLSTGTGFNSMSLT
                     NTLGALSHLIENGLEASSTYELVILNLRMPRTLAVIAVGVGLAVAGSAMQALIRNPLV
                     DPYITGVSSGAALGATLAMLAGITVTEISSYSIPIMAFLGAVGAFLFTMLLAEAAGGR
                     PINYVLGCMIVSIALQAGTTLLIYFNTDKLHGVLFWLFGSFAYVEWKSTLIIIIVVAV
                     LALVMLLFAKEFNVILLGDEQAKQLGMNVRRFKTTMFMIVSAMAAVCVAFTGVIGFVG
                     LIVPHLVRMVVGGDHRLLLPASMMLGANVLLVADIVCKTAVAPSELPIGAIISIIGAP
                     FFGYLMVRVGKEYVM"
     gene            complement(1835..>2039)
                     /locus_tag="PLJ11_07535"
     CDS             complement(1835..>2039)
                     /locus_tag="PLJ11_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04554.1"
                     /translation="EIGLESLLERDPEFLLISMMALPASPDQIIQDLKEGSLSSSASI
                     MADNSSVIGLGTDLIVGMKDALV"
BASE COUNT          477 a          576 c          517 g          469 t
ORIGIN      
        1 ccgtagctga ttatcccgcc gtcgcttatg atgatgacca tatcggcgta cttcgctatg
       61 aggtttatat cgtgcgaggc ggtgatcacc gtcaccccct gacgggagag gtccttgagg
      121 gcctgcatca cctgcagctt gaacttgagg tccaaatgag cgctcggctc gtccgccagc
      181 agtatcttcg gctgctgcac ggcccccttg gccaggagca cgcgcgcctt ctccccgctg
      241 ctgagctccg caagcttgcg gtccttgaga tgggtgacgt gataggcctc cattgcctcc
      301 cgcacgagcc gtacgtcctt ctccccctcc caccagatgc ccgtgacgta cggatatctg
      361 cccagggaga cgaactcctc tactgagagg tggaagtcgt cgggcacgtc cgcggggatg
      421 ttggcgcaga gcttcgccac ctccatcatc ctgaggcggt ccaggtcctt accgtcgatg
      481 tagatcgccc cctcctgggg cttgaggacc ttgttgatgc agcgcatgaa ggtcgtcttc
      541 ccgcacccgt tcgggccgac gatccctatc agctgcccct tcctggcgct gatggtgatg
      601 tccttcagcg tcctcccgca cgggttccgt ttggtgccgt agctgaagga gacgttatcg
      661 accctcaccg tcagctcgtc ctcacatgac atattcctta cccacccgga ccattagata
      721 accaaagaac ggcgctccga ttattgatat tatggccccg atcgggagct ccgagggggc
      781 caccgcggtc ttgcatacga tgtcggccac caacaggacg ttcgccccga gcatcatgga
      841 ggccgggagc aacagtcggt ggtcgccccc gacaaccatg cggaccaggt gcgggacgat
      901 gagcccgacg aagccgatga cgccggtgaa ggccacgcac accgcggcca tggcggagac
      961 tatcataaac atggtggttt tgaaccttcg gacgttcatt cctaattgtt tggcctgttc
     1021 atcccccagg agaatcacat tgaactcctt ggcaaaaagc agcataacta atgccaaaac
     1081 tgcaaccact atgatgatga tcagtgtgga tttccattct acataggcga aagaaccgaa
     1141 aagccagaat aatactccat gcaatttatc tgtgttaaaa tagatcaata gggtcgtacc
     1201 agcttggaga gcgatgctta cgatcataca acctagtaca taatttatgg ggcgacctcc
     1261 cgcagcctcg gcgaggagca tggtgaagag gaaggctccg actgcaccta agaacgccat
     1321 aatcggaatc gaatagctag atatttcggt aacagtaatc ccggccagca tagctaatgt
     1381 tgcacccaat gcagcccctg atgacacacc ggttatgtat gggtctacga ggggatttct
     1441 gatgagagcc tgcatggcac tccctgcgac agccagtcca acccccacgg ctattaccgc
     1501 taaagtgcgc ggcatcctta agtttaatat caccaactca taggtgctac ttgcctccaa
     1561 tccattttcg ataagatgag acagtgctcc aagtgtgttt gttagtgaca tgctgttgaa
     1621 gcctgttcct gttgatagtg aaagaattat aaggaaggcg aggctgatcg atccaagaat
     1681 aaatatcata agcagcttga tcttaggatg agcagccttt tttccttcca tcttatccct
     1741 catagtgacg ttgggcggtt tgctatcact accaccgtgg cgcagtgacc tctgcgtatc
     1801 gatgaccaat taataaatca gtgcttcatt acaactaaac caatgcatcc ttcataccga
     1861 cgatcaaatc cgttcctaga ccaatgacgc tgctattgtc cgccataatc gaagctgagg
     1921 aggacaagga gccctccttc aggtcttgaa taatctggtc gggagaggcg ggaagagcca
     1981 tcattgatat aaggaggaat tcgggatccc tttccaaaag agattctagc ccaatctct
//
LOCUS       DAOSYN010000241         2038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_41580_length_2038_cov_3.359556, whole genome shotgun sequence.
ACCESSION   DAOSYN010000241 DAOSYN010000000
VERSION     DAOSYN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41580_length_2038_cov_3.359556"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLJ11_07540"
     CDS             <1..788
                     /locus_tag="PLJ11_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04555.1"
                     /translation="SGEVEDEEGNRLAIGADGEWVMEIANGTPLPAFGDVMGSQYSGY
                     YMPAGNYTVELTGREEGSYNCVLFSGAKAAYAIENVEGGEGTHDTLRLFQRDGNPFMG
                     TMTYQTSDEEKGYSATMTKRFGERERVFKIINATLFEGDRAIINTTEDYCKLVFQNDG
                     DHSFAFDVRFQGNVLSAEAWERLNGTLTDLPTCEAFGIEIGPHETLTIYPSDWLDLES
                     AEVIVEREGDGGLDVLYIALLVAALVAAVAVLWYLAVGRKKRD"
     gene            complement(785..1531)
                     /locus_tag="PLJ11_07545"
     CDS             complement(785..1531)
                     /locus_tag="PLJ11_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOO04556.1"
                     /translation="MDSETEVARSIDGLREEIGGLSKAVLGVRFEDFKKVVLAQIQYV
                     ISDHYRHYLSSTLDRIDSLSACPLRKDCRRELDSIFQGISACFLRDDFSTAQQDLDRA
                     EALICGLRSPCQNRECHNLQVGMVQDVRSLIAFIQRIYERIGGQGAEGIARAEGDQLD
                     PGQVSRMVAPLAHPARLQILEALSLRDRSFTDLSRELDMRTGHLQFHLRPLLDEGYVS
                     KLRNRGDYSLTPRGRAALDLLAVFSQRLSV"
     gene            1594..1806
                     /locus_tag="PLJ11_07550"
     CDS             1594..1806
                     /locus_tag="PLJ11_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04557.1"
                     /translation="MNNVEILNFVQNASKEQLTALGTLGAWMQEKVPVYCTCPSKCNQ
                     KCQLAKELNEALALAGERLQAERARP"
BASE COUNT          414 a          578 c          690 g          356 t
ORIGIN      
        1 cctccgggga ggtcgaggac gaggaaggga accgcctggc cataggggcg gatggcgaat
       61 gggtgatgga gatcgcgaac gggacgcccc tgccggcgtt cggcgatgta atgggatccc
      121 aatactctgg ctactacatg ccggcgggca actacaccgt cgagctaacg ggcagggaag
      181 agggaagcta caactgcgtc ctgttcagcg gggccaaggc cgcctacgcc atcgagaacg
      241 tcgagggtgg cgagggaaca cacgacaccc tcaggctgtt ccaaagggac ggcaacccgt
      301 tcatgggcac catgacctac cagacctcgg acgaggagaa gggctactcg gccaccatga
      361 ccaagcggtt cggggagcgc gagagggtct tcaagattat caatgccacg ctcttcgagg
      421 gcgaccgggc gatcatcaac acgaccgagg actactgcaa attggtgttc cagaacgacg
      481 gggaccacag cttcgcgttc gacgtgcgct tccagggaaa cgtgctgagc gcggaggcgt
      541 gggagcgatt gaacggcacg cttaccgatc ttcccacctg cgaggccttc ggaatcgaga
      601 tagggccgca tgagacgctg accatctacc cgagcgattg gctggacctt gagtcggcgg
      661 aggtcatcgt ggagcgggag ggggacggcg gccttgacgt gctgtacata gcgctgctcg
      721 tcgcggcgct ggtggcggcc gtggccgtgc tgtggtacct tgcggtcggg aggaagaaga
      781 gggactaaac ggaaagcctt tgggagaaca cggccagcag gtccagggcg gcccttcccc
      841 ggggggtcag ggaatagtcc ccccggttcc tcagcttact tacgtaccct tcgtccagca
      901 acggccggag atggaactgc aggtgccccg ttctcatgtc cagctcccgg ctcaggtcgg
      961 tgaaggaacg gtcccttagg ctaagggcct cgaggatctg caagcgggcg ggatgggcga
     1021 gcggggcgac catccttgag acctgccccg ggtccagctg gtccccctct gcgcgggcga
     1081 tgccctcagc cccctggccg cctatccttt cgtagatcct ctggatgaag gcgatcaggg
     1141 atcgcacgtc ctggaccatg cccacctgca ggttgtggca ttccctgttc tggcaggggg
     1201 aacggagtcc gcagatcaac gcctcggccc tatccagatc ctgctgggcg gtgctgaagt
     1261 cgtccctcag gaagcaggcc gaaatgcctt ggaagatgga atcgagttcc ctgcggcagt
     1321 ccttgcgcag gggacaggcg gaaagggaat cgatgcggtc gagggtggag gacagatagt
     1381 gccggtaatg atcggagatc acatactgga tctgggcgag gacgaccttc ttgaagtcct
     1441 cgaagcgcac gcccagcacc gccttgctca ggccgcctat ctcctctctc agcccgtcga
     1501 tcgacctcgc cacctccgtc tcgctgtcca ttttagtcaa gtaagatatt aattcataat
     1561 attaatgtat tattatgagg ttctccgaac taggtgaaca atgtggagat attgaacttc
     1621 gtacagaacg cgagcaagga gcagttgacg gccctgggaa ccctgggggc atggatgcag
     1681 gagaaggtgc ccgtgtattg cacgtgcccc tccaagtgca accagaagtg ccagctggcc
     1741 aaggagctca atgaggcctt ggcgctggcg ggggagaggc tgcaggctga gcgagcacgc
     1801 ccgtagccct ccggaaaaga aggggtcgac gttaccctga ccgatccgcc ctgtcgtcgg
     1861 aacgtccatc ctcggatcgg ccaggtatgc tatgggagat ggccggtcga ggagcggtcc
     1921 acgcgacacc ggaggtggat gggcggtcga agggagctag agggagccag ggcatggccg
     1981 atcggcaggc cggcgcacct ctggacctcg gtggaaggct gcccatcgtg ctagtcgg
//
LOCUS       DAOSYN010000242         2028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_41836_length_2028_cov_4.673087, whole genome shotgun sequence.
ACCESSION   DAOSYN010000242 DAOSYN010000000
VERSION     DAOSYN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41836_length_2028_cov_4.673087"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PLJ11_07555"
     CDS             complement(<1..971)
                     /locus_tag="PLJ11_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04558.1"
                     /translation="MIVDSYDVVFGQLDPEEMRRVNRSRNYAQALISDPRDIYSAFDE
                     VEQEFGARKVKYAAQGYDPFSTFNPDAPSGVDPFTSATARDYAELLYPVIKAATVGAG
                     ENLLGSSYQAAGHMLSRINDLIDYAGKQLGIPRVELMDKMAKATASRGDEFRKMAKED
                     GIGPITNWFTEVLGQAPAGIAEFIPTITPVGTVAAMGIHGAMGAAEAEKAGENELIGF
                     MKGALERGLMGQVLHAASTLSRPLRMGVTGGVFEEQARHEGITDPEELFKAFATGSLF
                     GVVGGKGKYGPHEIRKELANLEMEARERIEGLDPRAVIGERGSNKGS"
     gene            complement(968..>2028)
                     /locus_tag="PLJ11_07560"
     CDS             complement(968..>2028)
                     /locus_tag="PLJ11_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04559.1"
                     /translation="LAIRNAAGDKKLIQDAVDRHLLNLNVIYHGQDVTDYKTGDASRL
                     NLEALTQLKHWQHTQVANELMTKFEGDHESMYRHLMDPNNYKELSIEEKHTIGNIITS
                     EFSKLKAFKKEEQAATAADLWAEYYEVRFGSRRGEKGALAQWHAKVADMTRRGLVDGH
                     VMQNAIALEAQEQASIRAATAAQRAQEEWERKNSPLLRDDPEVAARWTEMVIKDPASP
                     ELDKMSEYLGRGLSYKTFEHLSGLRKSKEKAIWATPNGKLVGNQLSEYRRLGKFSTDL
                     QKNNEEFTIAVKRMEKFLLRHPDATAEQINQEMNNLMRPYLRSTVLKMFDKLEDWWKS
                     PPKAGSQESPPNVLEDNP"
BASE COUNT          333 a          677 c          523 g          495 t
ORIGIN      
        1 taggacccct tattgcttcc cctttccccg atgacggccc tcgggtccaa cccctcgatc
       61 ctctctcggg cctccatctc caggttggcc agttccttgc ggatctcatg gggaccgtac
      121 ttcccctttc cccccaccac cccgaacagg gaccctgtgg cgaacgcctt gaacagctcc
      181 tcggggtccg taatgccctc gtgccgggcc tgttcctcaa acaccccgcc cgtgaccccc
      241 atgcgcaggg gacgggaaag ggtactcgcg gcgtgcagca cctgtcccat caggccccgc
      301 tcgagcgccc ccttcataaa cccgatgagt tcgttttccc cggccttctc ggcctctgct
      361 gcccccatgg ctccatgtat acccattgcc gccacggtac ccaccggggt gatagtaggg
      421 ataaactccg cgataccagc aggggcctga ccaagcacct cagtaaacca gttggtaata
      481 ggcccaatac cgtcttcttt cgccatcttg cgaaactcat cacctctcga ggcagtagct
      541 ttggccatct tgtccataag ctctactcga ggtatcccaa gctgcttccc ggcgtagtca
      601 atcaggtcat taatcctgga aagcatatga cctgcagcct gatatgacga ccctaacagg
      661 ttctctcctg cccccactgt agccgctttt attaccgggt aaagcagttc cgcatagtcc
      721 ctcgcggttg cgctagtaaa cgggtccacc ccgctgggcg cgtccggatt gaaggtagag
      781 aagggatcgt atccctgcgc cgcgtacttc accttccggg caccgaactc ctgctccacc
      841 tcgtcaaatg cggagtatat gtccctcgga tcggagatga gggcctgcgc gtagttgcgg
      901 gaccggttca ccctcctcat ctcctccggg tccagctgcc cgaacacgac gtcgtaggag
      961 tccactatca tggattatcc tccagaacgt taggaggtga ctcctgactc cctgccttgg
     1021 gaggtgactt ccaccagtcc tccagcttgt caaacatctt cagcacggtg gacctcaagt
     1081 acggcctcat caggttgttc atctcctgat tgatctgctc ggcggtggcg tccgggtgtc
     1141 ggagcaggaa tttctccatc ctcttgacgg cgatggtgaa ctcctcgttg ttcttctgca
     1201 ggtcggtgct gaacttgccc aacctcctgt actcggacag ctggtttccc accagcttcc
     1261 cgttgggcgt ggcccaaatg gccttctcct tggactttct cagcccactg agatgctcga
     1321 acgtcttgta cgacaggcct cttcccaggt actcggacat cttgtccagt tcggggctgg
     1381 ccgggtcctt tatcaccatc tccgtccagc gtgccgccac ctccgggtcg tcccggagca
     1441 gcgggctgtt cttgcgctcc cactcctcct gcgccctctg cgcagcagtt gcagcgcgga
     1501 tggatgcctg ttcctgcgct tccagggcta tcgcgttctg catcacgtgc ccgtcgacca
     1561 gacctcttcg ggtcatgtcg gccaccttcg cgtgccactg ggcaagagca cctttctccc
     1621 ctctccggga tccgaatctc acttcgtagt attcggccca caggtcggcg gcagtggcag
     1681 cctgctcttc cttcttgaac gccttcagct tggagaactc ggaggtgatt atattcccga
     1741 tcgtgtgctt ctcctcgatg gacaattcct tgtagttgtt ggggtccatg aggtgcctgt
     1801 acatggactc atgatccccc tcgaacttgg tcatgagttc gttggccacc tgcgtgtgct
     1861 gccagtgctt aagctgggta agggcctcca ggttgagtct cgatgcgtcc cccgtcttgt
     1921 agtccgtcac gtcctgcccg tggtatatca cgttcaggtt gaggaggtgc ctgtctaccg
     1981 cgtcctggat aagcttcttg tcccctgccg cgttgcgtat ggcgagaa
//
LOCUS       DAOSYN010000243         1998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_42658_length_1998_cov_3.424601, whole genome shotgun sequence.
ACCESSION   DAOSYN010000243 DAOSYN010000000
VERSION     DAOSYN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42658_length_1998_cov_3.424601"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PLJ11_07565"
     CDS             complement(<1..1185)
                     /locus_tag="PLJ11_07565"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO04560.1"
                     /translation="MTGPEHKIIHDSVHGSVKVSGVFLELLHRPELQRLHGVKQLGLA
                     YLVFPGANHTRFEHSLGTYYVAGLMCEALELSPEDRAQALAAALLHDLGHPPFSHTLE
                     EVLENRFSMDHMELGAALVRGQRSAVRDWEQGMIGCEGTIAELLERNGIPAEAVADII
                     VSPGHKARRGQRLITEASGQSHFNSRDHLNQMLHGPVDADQMDYLLRDAHYTGVAHGA
                     IDLDRLVHTIALHHGDIVVRKNGLVAAEGLMVARALMYSSVYFHKTVRIAEMMLCKAV
                     EAADDSIAEGLQQENDPSLLERLVAQGGRPRKLVTLLKYRHLYKRAFSVYVPDMTEED
                     AERLLPITEYRKRKATEALIADRAGVDPSEVILDAPSRELITGEARRGKTEVPILNDG
                     RVR"
     gene            complement(1220..1597)
                     /gene="sepF"
                     /locus_tag="PLJ11_07570"
     CDS             complement(1220..1597)
                     /gene="sepF"
                     /locus_tag="PLJ11_07570"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOO04561.1"
                     /translation="MPILKKPMGKGKEDVKSVETDQYIDLGALYFPDESTVSAGGIKV
                     AEIYRYEDVGPITQPVYQGNILMIDYSPIANDQNTLKRITNELKSVARDTNGDVAAIG
                     NNIIVVAPNGIRIDRKKIKGGYQ"
     gene            complement(1674..>1998)
                     /locus_tag="PLJ11_07575"
     CDS             complement(1674..>1998)
                     /locus_tag="PLJ11_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOO04562.1"
                     /translation="GMIVDGQPFPTIRGLLRHKATKRYVTVDMGAVPFVTNGADVMAP
                     GIVEADPEVREGDLVWIRDVTHERPLAIGRALVPAEVMATKAPGKAIENLHFVGDKIW
                     KYDER"
BASE COUNT          306 a          671 c          620 g          401 t
ORIGIN      
        1 cctgaccctg ccgtcgttca ggatcggcac ctccgtcttc ccccgccgcg cctcgccggt
       61 gataagctcg cgggagggcg cgtccagtat gacctcggac gggtccaccc ctgcccggtc
      121 cgcgatcagg gcctcggtgg ccttgcgctt ccggtactcg gtgatgggaa gcagacgctc
      181 ggcgtcctcc tcggtcatgt ccggcacgta gaccgagaac gcccttttgt acagatgacg
      241 gtacttaagc aacgtgacca gttttcgggg gcggccccct tgggcgacca accgctccag
      301 gagggagggg tcgttctcct gctgcagccc ctcggcgatc gaatcgtcgg cggcctccac
      361 cgccttgcat agcatcatct ccgcgatgcg caccgtcttg tgaaagtata ccgaggagta
      421 catgagcgcc cgggcgacca tcaggccctc cgcggccaca agcccgttct tgcgcaccac
      481 gatgtccccg tggtgcaggg cgatcgtatg gaccaggcgg tccaggtcga tggcgccgtg
      541 cgcgacgccg gtgtagtgcg cgtcgcgcag caggtagtcc atctggtccg cgtccaccgg
      601 gccgtgcagc atctggttca gatgatccct ggagttgaaa tgggactgcc cgctggcctc
      661 ggtgatcagc cgctgcccgc gccgggcctt gtgccccggg gacacgatga tgtccgcgac
      721 cgcctcggcc gggatgccgt tccgctccag gagctcggcg atggtcccct cgcatccgat
      781 catcccctgt tcccagtccc ggaccgcgct tcgctgtccc ctaaccagcg cggcgccgag
      841 ttccatgtgg tccatggaga accggttctc caacacctct tcgagggtgt gggagaaggg
      901 gggatgcccg aggtcgtgca gcagggcggc cgccaaagcc tgcgcgcggt cctcggggct
      961 cagctccagt gcctcgcaca ttaggcccgc cacataatag gtgcccaggg aatgctcgaa
     1021 gcgggtgtgg ttcgcccccg ggaacaccag gtacgcaagg ccgagctgct tgacgccatg
     1081 cagccgctgc agctccgggc gatgcaacag ttccaggaag accccgctga ccttcacgct
     1141 tccgtgcacg ctgtcgtgga ttatcttatg ctcaggcccg gtcatggaac ggagggcccc
     1201 ttgcgctggg acaggatgcc tactggtagc cgcccttgat cttcttccgg tcgatgcgga
     1261 tgccgttggg ggccaccacg atgatgttgt tcccgatggc ggccacgtcc ccattcgtgt
     1321 ccctggccac cgacttcagc tcgttggtga tgcgcttgag ggtgttctgg tcattggcga
     1381 tgggggaata atcgatcatc aggatgttgc cctgatagac cggctgggtg atcgggccca
     1441 cgtcctcgta tcgatagatc tcggccacct tgatgcctcc ggcggagacg gtgctctcgt
     1501 ccgggaagta cagggcgcca aggtcgatgt actggtcggt ctccacgctc ttgacgtcct
     1561 ccttgccctt gcccatcggc ttcttgagaa tcggcattgc atccctcggt cggacagtta
     1621 tggcatctaa tcaatattaa tctatctcaa tcaatcctgg accgggcggg gcctcatcgc
     1681 tcgtcgtact tccagatctt gtcgcccacg aagtgcaggt tctcgatcgc cttcccgggg
     1741 gccttggtgg ccatgacctc tgcgggcacg agcgcccttc ctatcgcaag agggcgctcg
     1801 tgcgtcacgt cccggatcca gacgaggtcc ccctccctca cctcggggtc cgcctccact
     1861 atgcccgggg ccatgacgtc cgccccgttg gtcacgaacg ggacagcgcc catgtccacc
     1921 gtgacatagc gtttggtggc cttgtgcctg agcagcccac ggatggtcgg gaacggctgc
     1981 ccgtccacga tcatgccc
//
LOCUS       DAOSYN010000244         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_43745_length_1960_cov_1.843570, whole genome shotgun sequence.
ACCESSION   DAOSYN010000244 DAOSYN010000000
VERSION     DAOSYN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43745_length_1960_cov_1.843570"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..409)
                     /locus_tag="PLJ11_07580"
     CDS             complement(80..409)
                     /locus_tag="PLJ11_07580"
                     /inference="COORDINATES: protein motif:HMM:NF039801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6506 family protein"
                     /protein_id="HOO04563.1"
                     /translation="MSENKFITAFVAMSPDADPGKHRCVIETSVQRLYSVLVRDERQA
                     EEVCAELVRKEGVCSIILCPGFTNQAVGRLSEALGRDVAVNVARGDGPSGAVAHRAME
                     RAGFLRH"
     gene            complement(479..781)
                     /locus_tag="PLJ11_07585"
     CDS             complement(479..781)
                     /locus_tag="PLJ11_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04564.1"
                     /translation="MVAPSLASLAFQGSALGIGRLRQAPFLASRASERSCGVRRSQRR
                     GAGRRSIPRLSSPASSNTCVYAPVGRPSEIGSPTIAPVQEAARRSRSTQATLSLAG"
     gene            919..>1960
                     /locus_tag="PLJ11_07590"
     CDS             919..>1960
                     /locus_tag="PLJ11_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04565.1"
                     /translation="MRVALLSLAAAAALLVLSIAPSSSASAPEWEELGDMPVGLTHAT
                     CVELLDGRVLVTLGGDGDEVPSDLTWLYDPKTDGWSEGADAPAPFMGAEGVAMPDGKV
                     YVFGGLWMDQDTMTLVRQTEVLIYDVGDDTWTTGTAVPGQLSLTEVAALDDERVLLAG
                     GMDADMQATDACLIYDTVSGAFEAAAPLPAPRNTGVAFAYQGDAYYAGGSDAASLMAM
                     PEVFRYDVPSGTWSLYGKMPEGRYYDEGAMGDDGLLYIYGGKTGATTDLTGTGTVWIV
                     DMADWIQRNTPDVFAAITQGAALPAGSGQAQFLTELERLKIKVTRPGGAKPTITLRTA
                     IFELRNRLGLVQG"
BASE COUNT          317 a          639 c          691 g          313 t
ORIGIN      
        1 tcctcgaggt ccagggaggc catgtccagc tcaggcggcc actccccacg ggcatcttcc
       61 ctgtgagggg ggccgtccct cagtgcctga ggaatccggc cctctccatg gcccgatggg
      121 ccacggcgcc gctcggaccg tcgcccctcg ccacgttgac cgccacgtcc cttcccaacg
      181 cctcggacag ccttcctact gcctggttgg tgaacccggg gcagaggatg atggagcaca
      241 ccccctcctt ccgcaccagc tcggcgcata cctcctcggc ctgccgctcg tccctcacca
      301 gcacgctgta aagcctctgg acggaggtct cgatcacgca tcgatgcttc ccggggtcgg
      361 cgtccgggct catggccacg aaggcggtga tgaacttgtt ctcgctcatg ggggatagat
      421 cgtccggcgg gataataaat catgaaggcg agggcgagta gggcatcggc ggggcgacct
      481 atccggccag ggacagcgtg gcctgggtgg agcgggatct cctcgccgct tcctggacgg
      541 gagcgatggt cgggctgccg atctccgaag gcctgccgac aggggcatac acgcaagtgt
      601 tcgacgacgc cgggctggac agcctgggaa tggaccggcg gcctgccccc cggcgctggc
      661 tgcgacggac cccgcaggac cgttcggacg ccctggaggc gaggaacggg gcctgccgga
      721 gccgtccgat gcccagggcc gagccctgga aggccaggga cgccaggctg ggcgcgacca
      781 tcgggtgcat gtcccgatcc cggtggtcat accggggtgg gcgtgccggt cctggtgcgt
      841 gaggggcgat agccttccgt tcatcatttt tatatgctca ccgggaccta atcacattga
      901 catcggagga ggagcaccat gcgtgttgca ttgcttagcc tggccgccgc ggcggcgttg
      961 ctggtcctgt cgatagcacc gtcgtcatcg gcgtccgcgc cggaatggga ggagctgggg
     1021 gacatgccgg tgggattgac gcacgccaca tgcgtggagc tgctggacgg cagggtgctg
     1081 gtgaccttgg gcggggacgg cgacgaggtc ccttcggacc tcacctggct atatgaccct
     1141 aagacggacg gatggagcga gggggccgac gcccccgccc ccttcatggg cgccgagggg
     1201 gttgcgatgc ccgacggcaa ggtgtacgtc ttcggcgggc tctggatgga ccaggacacc
     1261 atgacccttg tgcgccagac cgaggtcctc atctacgacg tcggggacga cacctggacc
     1321 accgggacgg cggtgccggg gcagctcagc ctgaccgagg tcgcagcgct ggacgatgaa
     1381 agggtccttc tggcgggagg aatggacgcc gacatgcagg ccacggacgc ctgcctcatc
     1441 tatgacaccg tgagcggggc cttcgaagcg gccgccccgc tccccgctcc caggaacacc
     1501 ggcgtggcct tcgcttacca aggggacgcc tactatgccg gagggtcgga cgccgcctcc
     1561 ctgatggcca tgccggaggt gttccggtac gatgtgccct cggggacatg gtccctgtac
     1621 ggcaagatgc ccgaaggacg ttactacgac gagggggcga tgggcgatga cggcctgctg
     1681 tacatttacg gggggaagac cggggcgacc accgacctca ccgggaccgg gacggtctgg
     1741 atcgtggaca tggccgactg gatccagcgc aacacgcccg atgtctttgc ggccatcacg
     1801 cagggcgcgg cgctgcccgc cggctcgggc caggcgcagt tcctgaccga actggagcgg
     1861 ctgaagatca aggtgacgcg ccccggcggc gccaagccga cgatcacgtt gcgcaccgcg
     1921 atcttcgagc ttcgcaaccg gctgggcctg gtccaaggcg
//
LOCUS       DAOSYN010000245         1954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_43900_length_1954_cov_1.823065, whole genome shotgun sequence.
ACCESSION   DAOSYN010000245 DAOSYN010000000
VERSION     DAOSYN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43900_length_1954_cov_1.823065"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLJ11_07595"
     CDS             <1..502
                     /locus_tag="PLJ11_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04566.1"
                     /translation="PAEGFVEAHLREHSDPRVLAVRGRCLPRTRNLPNRLAFHYDLGP
                     ERIPSPIDLEGNSSFKRAELLACGGFNETLPLAGGWEGVDLSYRLVMRSGDPASVLYS
                     PGPTIRHDYKANAFELLTKLVRHRRNLGKMSQGQVDLNAFLLRYERERNPLQKMRRAL
                     LVYRSP"
     gene            complement(487..1695)
                     /locus_tag="PLJ11_07600"
     CDS             complement(487..1695)
                     /locus_tag="PLJ11_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04567.1"
                     /translation="MARKVVVLDLHTFYDNRINKHIALVGQRYAVLRINVNLFHGRRV
                     EGTQGYQAHIIDFTPTRGQYLNGLLFTLGTLLGTVPRRLHRRLREDFLQEGDEVLIHV
                     HDPYLLGLAVRLSGRLPGSRVVYDRHDHFETWKNRLGVSVPGLFERWYGRRVEELIIA
                     NPGTEGLPKALSGKRVTQVPNYPLSRYFDREAVERKAAALGPESGMDLAYFGALSLGF
                     DRDIDLMLRLMAALMREDPRTTCTVAGRIFERGVAGRLEEMRAEFGERFRYLGELPYQ
                     EVIARSKEAHIGFFLMNPERPTWSPRRPYSANKIYEYLQTGTVPVVRAVIEEPEGVRD
                     CSLFFDERSTFDEMLGSLRALCADRERLRRMVLQCFETGLKYSWEEVSPRYLECYERV
                     FEKVGAQGER"
BASE COUNT          326 a          679 c          623 g          326 t
ORIGIN      
        1 ccctgccgag gggttcgtgg aggcgcacct gagggagcat tcggacccgc gggtgctcgc
       61 ggtgagagga aggtgcctgc cgaggacccg caacctcccg aaccgcctgg cgttccatta
      121 cgacctcggg ccggagcgca tcccctcccc gatcgacctg gaagggaact cctccttcaa
      181 acgggccgag ctgctggcct gcgggggctt caacgagacg ctccccctcg ccggcggatg
      241 ggagggcgtg gacctctcct accgcctggt catgcggagc ggggaccccg cctccgtgct
      301 ctactccccc ggcccgacga tccgtcacga ctacaaggcc aacgccttcg agcttctgac
      361 caagctggtc aggcaccgga ggaacctggg gaagatgtcc caggggcagg tggacctgaa
      421 cgccttcctc ctccggtacg aaagggaaag gaacccgttg caaaagatgc gaagggcgct
      481 gctggtctac cgctccccct gagcgccgac cttttcgaac acccgctcgt agcactccag
      541 gtaccggggg gagacctcct cccaggagta cttgaggccg gtctcgaagc attggaggac
      601 catccgcctc aaccgttccc ggtcggcgca cagggcccgc agggagccga gcatctcgtc
      661 gaacgtgcta cgctcgtcga agaagaggga acaatcgcgc accccctccg gctcctcgat
      721 caccgccctg acgaccggga ccgtccctgt ctgcaggtac tcgtagatct tgttggccga
      781 gtacggccgg cgcggggacc acgtcggccg ctccgggttc ataaggaaga agccgatgtg
      841 tgcctccttc gagcgggcga tcacctcctg gtacggcagc tcgcccaagt agcggaaccg
      901 ctcgccgaac tccgccctca tctcctccag tcggcccgcg acgccccgct cgaagatccg
      961 gcccgcgacc gtgcaggtgg tcctcgggtc ctcgcgcatg agcgcggcca tcagccggag
     1021 catcaggtcg atgtcgcggt cgaagcccag gctcagggcc ccgaagtagg ccaggtccat
     1081 cccgctctcc gggccaagcg ccgcggcctt ccgctccacc gcctcccggt cgaagtacct
     1141 tgacagagga tagttgggga cctgcgtgac ccgcttcccc gagagggcct tgggcagccc
     1201 ctccgttccg gggttggcga tgatgagctc ctccaccctc ctcccgtacc accgctcgaa
     1261 gagccccggg acggagacgc ccagccggtt cttccacgtc tcgaagtgat cgtgccggtc
     1321 gtagacgacc cttgagccag ggagccttcc gctcaatcgg accgccaggc cgagcaggta
     1381 ggggtcgtgc acgtggatga gcacctcgtc cccttcctga aggaagtctt cgcgcagccg
     1441 ccggtgcagg cggcggggca cggtcccgag gagggtgcca agcgtgaaga gcagtccgtt
     1501 caggtactgc cccctcgttg gggtgaagtc gatgatatgg gcctggtacc cctgtgtgcc
     1561 ctccaccctc cggccgtgga agaggttgac gttgatgcgg agcacggcat agcgctgccc
     1621 cacgagcgcg atgtgcttgt tgatgcgatt gtcgtagaag gtgtgcaggt ccaggaccac
     1681 gacctttcgt gccacgggat agccgattcg aaaggtcggt atattaagct atggcggctc
     1741 ccgtcccccc tctcgcgccc ccgctcccat cccggtgcgg tccctgcggt gcgagctggt
     1801 cgtttccctt agcaaatgac ccatcgggaa caggccctat gcggggaggg aacgctcggc
     1861 cgccggccat ggcgtacaaa gggagacgca ccgggcgcct ctgacgccgc ctcggcatcg
     1921 cctatttatc catggtagca catggccccc tggg
//
LOCUS       DAOSYN010000246         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_44911_length_1920_cov_4.452547, whole genome shotgun sequence.
ACCESSION   DAOSYN010000246 DAOSYN010000000
VERSION     DAOSYN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44911_length_1920_cov_4.452547"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1005)
                     /locus_tag="PLJ11_07605"
     CDS             complement(31..1005)
                     /locus_tag="PLJ11_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOO04568.1"
                     /translation="MFPQGLPAYMGNLNVAAVGALGFAKELGKKGTVSDITVYDIKKG
                     HDTVSILEPSKYPERISSLYFCVNLADMALVVVDEVNAALGETILMLDCAQVDRGILI
                     LRNYVTPDRIAPLIKGTVLANYTHLPDDPIQVRELLLDEAAKVKAHGEGKGVVPIDHH
                     FNVKGVGTVILGLVAKGSVNRHDTLRVLPTDRTALVRSIQKHDDDFEVAYEGDRVGFA
                     LKGVEAEELDRGYVLTNDPEVRCVSELEARLDLVKYWQAPIKEGMVLHLGHWMQFLPC
                     RVTAVSGEPRSPHIMLSLERPLVHTPGSRAVLHYLEGGKLRIVGTMDL"
     gene            1058..>1920
                     /locus_tag="PLJ11_07610"
     CDS             1058..>1920
                     /locus_tag="PLJ11_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04569.1"
                     /translation="MLEHVDPGKRLDQKGYDKAAPGLKERLGELQRRARDAGMPIIVI
                     FEGWDTVGMSEIVNRFILPLDPRGFLVHPITAPSPEERARPFLWRFMVRIPARGRIAV
                     FDRSWYFRSLARAVERKEEDARAVWREIADFEEMLSNDGYLIMKFFLHIGKKEHRKRL
                     ERYRGSDMDMCAISMAEVELFKKYDRMLPLIEEMLEATDREHAPWTIVEAEDQRFASG
                     KVLASSVQMVERGLASREGRSQASMDGLAGDDRQFSSLRAKADLAVSIPQEEYRARLE
                     ELQGRVAELQCA"
BASE COUNT          340 a          606 c          625 g          349 t
ORIGIN      
        1 catcctgcac cggacggcct tcgggatcga ctagaggtcc atcgtgccca ctatccgaag
       61 cttgccgcct tccaggtagt gcaggaccgc ccttgagcct ggggtgtgga ccaagggcct
      121 ctccaggctc agcatgatat ggggggagcg gggctccccc gaaaccgcgg tcaccctgca
      181 gggcaggaac tgcatccaat gccctagatg cagcaccatg ccctccttga tgggggcctg
      241 ccagtatttg acgaggtcca gcctggcctc gagctccgat acgcacctca cctcggggtc
      301 gttggtcagc acgtatcccc ggtccagctc ctccgcctct acgcccttca gggcgaaccc
      361 cacccggtcc ccctcgtagg cgacctcaaa gtcgtcatca tgcttctgga tggacctgac
      421 caacgcggtg cggtccgtgg ggagcaccct caaagtgtca tgtcggttca ccgagccctt
      481 ggccacgagg ccgaggatga ccgtgcccac gcccttgacg ttgaaatggt ggtctatggg
      541 gaccaccccc ttcccctctc cgtgcgcctt caccttcgct gcctcgtcca ggaggagttc
      601 gcgcacctgg atgggatcgt ccgggaggtg ggtgtagttg gcgaggacgg tgcccttgat
      661 caggggggcg atgcggtccg gggtcacgta gttgcgaagg atgaggatgc cccggtcgac
      721 ctgggcgcag tccagcatga ggatggtctc cccgagggcg gcgttcacct cgtcgaccac
      781 caccagggcc atgtccgcca ggttcacgca gaagtacagc gagctgatcc gttccgggta
      841 cttggaaggc tccaggatgc tgacggtatc gtggcccttc ttgatgtcgt agaccgtgat
      901 atcgctgacg gtgcccttct tgcccagctc cttggcgaac cccaaggccc ccaccgccgc
      961 cacgttcaga ttgcccatgt acgcaggcaa gccctggggg aacatatgcc tttcgccgag
     1021 ccaagcgtta tctatcctgc caggccatta tcatcggatg ctcgagcatg tggacccggg
     1081 caagcgcctg gaccagaaag ggtacgacaa ggccgcccct ggcctcaagg agcgcctggg
     1141 ggaactccag aggcgtgccc gggacgccgg catgccgatc atcgtgatct ttgagggctg
     1201 ggacaccgta ggcatgagcg agatcgtgaa ccggttcatc ctccctctgg acccccgcgg
     1261 gttcctggtg catccgatca ccgcccccag tcccgaggag agggcgaggc ccttcctgtg
     1321 gaggttcatg gtccgcatcc cggcccgtgg aaggatcgcc gtgttcgacc gctcctggta
     1381 cttcaggtcc ctggccaggg cagtggagcg gaaggaggag gacgcccgag cggtctggcg
     1441 ggagatcgcg gacttcgagg agatgctctc caacgacggc tacctcataa tgaagttctt
     1501 tctgcacatc gggaagaagg agcacaggaa gcgattggag aggtatcgcg ggtccgatat
     1561 ggacatgtgc gccatcagca tggccgaggt ggagctattc aaaaagtacg atcggatgct
     1621 gccgctcatc gaggagatgc tcgaggccac ggaccgcgag catgcgccct ggacgatcgt
     1681 ggaggccgag gaccagcggt tcgcctccgg caaggtcctc gcctcctcgg tccaaatggt
     1741 ggagcgcggc ctcgcctcgc gggaggggcg gtcccaggcc tccatggatg gcctggctgg
     1801 ggatgaccgc cagttcagct ccttgcgcgc gaaggccgac cttgccgtaa gcattcccca
     1861 ggaagagtac agggctaggc tggaagagct tcagggcagg gtcgcggagc tgcagtgcgc
//
LOCUS       DAOSYN010000247         1916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_45039_length_1916_cov_4.736163, whole genome shotgun sequence.
ACCESSION   DAOSYN010000247 DAOSYN010000000
VERSION     DAOSYN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45039_length_1916_cov_4.736163"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..638
                     /locus_tag="PLJ11_07615"
     CDS             129..638
                     /locus_tag="PLJ11_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOO04570.1"
                     /translation="MTDSMDILGILAAARRIEEFGIEFYRRFSECTGDETGAALLRGL
                     GRDEEGHKAMVEEEMRRLAPALDPASVPPAPSALGIAPEKAFPFPPDRCMALEDELRA
                     LEIGIQVEESSSDMYRRAALSVEEPRVRLLLERLAGIEEGHRRLLEESMYMLRTQGAW
                     YGYTPILEG"
     gene            814..>1916
                     /locus_tag="PLJ11_07620"
     CDS             814..>1916
                     /locus_tag="PLJ11_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOO04571.1"
                     /translation="MVKLPNIEREISACLQCGYCIRTCETWRQTPWESVTPRGKIFYI
                     SQLGKRTVLDRLLGRKVEIDDEFVEALYMCTTCAACASVCHVGIDFAEFWEKVREWCI
                     ENGQGPMPAHVKIRERLEESRNPYGEPMEKRGAWYPDVPKADNPDVVFFAGCTASYRM
                     QNLAKASVVFLNRCGVSTNIMEGDEWCCTSPALRTGQTGLTYRFSEHNISTVEHMGAK
                     AMVTACAGCYKTTVHDYGKYYAHPTFEVFHFAQYANRLLKEKRVKFTKELKAKVTYHD
                     PCHLGRHAKVYDEPREVIRAIPGVEFVEMKNIRENSRCCGAGGGYKSQYNDMAIAIAI
                     ERVKEAVATGADILATTCPFCVLNLQQAAKKIGA"
BASE COUNT          406 a          551 c          630 g          329 t
ORIGIN      
        1 tcgatgggcg ccgcaccatg gaaagcctca taaacatttc catctgacca aaaatccatt
       61 atgcgagggg cgagagggct ggcaaaagca ttatcagcgc cggcagtgca tagctcacag
      121 gagctatgat gacggacagc atggacatcc tggggatcct ggccgcagcg aggcgaatag
      181 aggagttcgg catcgagttc taccgccgct tcagtgagtg caccggggac gaaacggggg
      241 cggccctcct tcggggattg ggacgggacg aggaagggca caaggcgatg gtggaggagg
      301 agatgcgccg gctggcgccc gccctggacc cggccagcgt gccgcccgcc ccctcggcct
      361 tggggatagc ccctgagaag gccttcccct tccccccgga ccggtgcatg gccctggagg
      421 acgagctgcg agcgctggag atcgggatcc aggtggagga atcctcctcc gacatgtacc
      481 gtcgcgccgc cctcagcgtg gaggagccga gggtgcgcct cctgctcgag cggctcgcgg
      541 ggatagagga agggcaccgc aggctcctcg aggagagcat gtacatgctg cgcacccagg
      601 gggcgtggta cggttacacg ccgatattgg aggggtaggc tgggaacgga ggcccgttat
      661 aaaaataaaa taatgcatat tttatttccc gtcccctttg gccatcacga tggatgggct
      721 gaaaaggctt taatacccgt cgggcatgca tagtgcacga tctgaacagg gtcggccccg
      781 gcccatcccc tcactggcct cagaggtatc acaatggtta agttaccgaa catcgagaga
      841 gagatttcag cttgtcttca atgcggctat tgcattcgca cctgcgagac gtggaggcag
      901 acgccctggg agtccgtcac tcccagggga aagatcttct acatatccca attgggcaag
      961 cgcaccgtcc ttgacaggtt gctgggccga aaggtggaga tcgacgatga gttcgtggag
     1021 gccctgtaca tgtgcacgac ctgcgccgcc tgcgcaagcg tatgccatgt ggggatcgac
     1081 ttcgccgagt tctgggagaa ggtgcgggag tggtgcatcg agaacggcca gggcccgatg
     1141 cccgcccatg tgaagatccg ggagcgcctg gaggagtccc gcaatcccta cggggagccg
     1201 atggagaaga ggggcgcctg gtatcccgac gtcccgaagg ccgacaatcc cgacgtggtg
     1261 ttcttcgccg gctgcaccgc ctcctaccgt atgcagaacc tggcgaaggc cagcgtcgtc
     1321 ttcctgaacc gctgcggcgt ctcaaccaat atcatggagg gcgacgagtg gtgctgcacc
     1381 tcccccgccc tgaggacggg gcagacggga cttacctatc gtttctcgga gcacaacatc
     1441 agcacggtcg agcacatggg cgcgaaggcg atggtcaccg cctgcgcggg atgctacaag
     1501 accaccgtgc acgactacgg caagtactac gcccatccga cctttgaggt gttccatttc
     1561 gctcaatatg ccaacaggct gctgaaggag aagcgggtca agttcaccaa ggagttgaag
     1621 gccaaggtca cctaccatga tccttgccac ctcggccgtc acgccaaggt ctatgatgag
     1681 ccgcgcgagg tcatcagggc gatcccgggc gtggagttcg tggagatgaa gaacatacgg
     1741 gagaactcga ggtgctgcgg cgctggcggc gggtacaaga gccagtacaa cgacatggcc
     1801 attgccatag ccattgagcg ggtcaaggag gccgtggcga cgggggcgga catcctggcg
     1861 accacctgcc ccttctgcgt gctcaacctg cagcaggcgg caaagaagat aggggc
//
LOCUS       DAOSYN010000248         1911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_45192_length_1911_cov_3.546336, whole genome shotgun sequence.
ACCESSION   DAOSYN010000248 DAOSYN010000000
VERSION     DAOSYN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45192_length_1911_cov_3.546336"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLJ11_07625"
     CDS             <1..194
                     /locus_tag="PLJ11_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOO04572.1"
                     /translation="ENYDRCLYVDTDVGDGERYEVLSRKFAEELGLRHENRPCGTANL
                     AKFLVRAREMARANARKAQ"
     gene            232..1356
                     /locus_tag="PLJ11_07630"
     CDS             232..1356
                     /locus_tag="PLJ11_07630"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOO04573.1"
                     /translation="MMNGDFKVTPWEVTGEIDYDQLITRFGTKKVEQDLLDRMAKYGE
                     LHPKLRRGVVYSHRDFDWLLDQYDAGNRFFLYTGRGPSGGTHLGHLMPWMFNRYLQDL
                     FKVNMYFQLTDDEKFLFNENLSLNDTKEAAYENALDFIALGFEPESTKVIIDTKDIGL
                     LYPLALQVAKRVTFSTAKAVFGFDNSNNIGSIFYTSIQSAPCFIEGALYGKRTPCLIP
                     CGIDQDAHFRVARDVAPLLGYPKPALIYNKMFPSLTGADKMSSSQPQTTIFTTDRPKE
                     VKRKIGNAFTGGQVSVEEQRRLGGKPEVCSVFKYEYYLFEKDDRALDELADRCRRGEV
                     LCGECKQLLIQRINAFLEEHQRKREEAKERLHEYLLPSTS"
     gene            1371..1772
                     /gene="tsaA"
                     /locus_tag="PLJ11_07635"
     CDS             1371..1772
                     /gene="tsaA"
                     /locus_tag="PLJ11_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOO04574.1"
                     /translation="MVSVHGDVSEIEVRPEFAEGLYRIELLKELDVLFLFDRSEGFDL
                     KVHPKGDPSIPLTGLFGTRSPRRPTPIGLTRVELLERKGNVLTVRGLDAFPGTPVVDI
                     KGAMGKGFSWDPNEMRGAVRRAPVRRARKSP"
BASE COUNT          402 a          544 c          629 g          336 t
ORIGIN      
        1 ttgagaacta cgatcgctgc ctttacgtgg acactgacgt cggggacggg gagcgctatg
       61 aagtgctgtc ccgcaagttc gccgaggagc ttggcctccg tcacgagaac cggccctgcg
      121 gcacggccaa cctcgccaag ttcctggtcc gggcgcggga gatggccagg gcgaacgcgc
      181 gcaaggctca ataagccttt tgaaccagga agccgttcct gccgcctgga aatgatgaac
      241 ggcgacttca aggtcacccc ctgggaagtg acgggggaga tcgattacga ccagctgatc
      301 accaggttcg gcaccaagaa ggtggagcaa gacctgctgg accgcatggc caagtacggg
      361 gagctgcatc cgaagctccg tcggggcgtc gtctactcgc atcgcgactt cgattggttg
      421 cttgaccagt acgatgcggg caacaggttc ttcctgtaca cgggccgggg accttcggga
      481 gggacccatc tggggcatct aatgccgtgg atgttcaacc ggtacctaca agacctgttc
      541 aaggtcaaca tgtacttcca gctcacggac gacgagaagt tcctcttcaa cgagaatctg
      601 agcttgaacg acaccaagga ggccgcctat gagaacgcgc tggacttcat cgccctgggc
      661 ttcgagccgg agagcaccaa ggtgatcatc gacaccaagg acatcgggtt gctctatccg
      721 ctggccctgc aggtggccaa gcgggtgacc ttctccacgg ccaaggcggt gttcggcttc
      781 gacaacagca acaacatcgg gagcatattc tacacgagca tccaatcggc gccctgcttc
      841 atcgaggggg ccctctatgg caagaggacg ccctgcctca ttccctgcgg catcgaccag
      901 gacgcccact tccgggtggc cagggatgtc gccccattgt tggggtaccc caagccggcg
      961 ctgatctaca acaagatgtt tcccagcctt accggggcgg acaagatgtc gtcctcccaa
     1021 ccgcagacca cgatattcac cactgaccgg cctaaggagg tcaagaggaa gataggcaac
     1081 gccttcaccg gcgggcaggt ctcggtggag gagcagcgga ggctgggagg caagcccgag
     1141 gtctgttccg tgttcaagta cgagtattat ctcttcgaga aggatgacag ggcgttggat
     1201 gaacttgcgg acaggtgccg caggggggag gttctgtgcg gggagtgcaa acagttgctg
     1261 atccagagga taaacgcgtt cctggaggag catcagagga agagggagga ggcgaaggag
     1321 cgcctccacg agtacttgct cccgagcacc tcctgaccgt catcggtcgg gtggtctcgg
     1381 tgcatgggga cgtctcggag atagaggtgc gcccggagtt cgcggagggg ctctaccgga
     1441 tcgaactgct gaaggagctg gacgtccttt tcctcttcga ccgcagcgaa gggttcgacc
     1501 tcaaggtgca ccccaaggga gacccgtcca taccgctcac cgggctgttc gggacccgga
     1561 gcccccggag gccgaccccg atagggctga cgagggtcga gctgctcgag cggaagggga
     1621 acgtgctcac tgtcaggggc ctggatgcgt tccctggaac cccggtggtg gacatcaagg
     1681 gagctatggg caaggggttc tcctgggatc ccaatgagat gcggggggcg gtccgcaggg
     1741 cgcccgtacg ccgggcccgt aagagcccat aagtaacata tatcgacgac caaatcccgt
     1801 aggacatgaa cgccgccgag gtgttggaag gcttgagccc ttccgagaag aaggtcctgg
     1861 tggccctgca gaggttggac ggcagggcat cgcctgagag gatcttcgag g
//
LOCUS       DAOSYN010000249         1898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_45570_length_1898_cov_4.902333, whole genome shotgun sequence.
ACCESSION   DAOSYN010000249 DAOSYN010000000
VERSION     DAOSYN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45570_length_1898_cov_4.902333"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLJ11_07640"
     CDS             <1..242
                     /locus_tag="PLJ11_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04575.1"
                     /translation="IGLALGFDPEGYQGLHRFILPLAALKGMTITEVPIVHYARPSGS
                     SYIKFYTVPFITLRDYSKFKKDHKDEIATLRGKGR"
     gene            252..1493
                     /locus_tag="PLJ11_07645"
     CDS             252..1493
                     /locus_tag="PLJ11_07645"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOO04576.1"
                     /translation="MSLEGRWQRLHAWLRALSPARKLAFYAIITLLVYGTTIVVDNAV
                     LVSMLDEDLSHPDLGVYRARAQTILDGGLLYRDVHTETPPVINYLLVPPQLLGGAEHD
                     WVWSIYFSLFAFLLAALMYLGLRPFDEDKAYLAGLLVLLSPYTIVESGTGEDESIVAF
                     VFMLSVVLMQLRKKHLASASIGVGVWTKMFPILLFPVHFLQQRNWRDRILSVAIVGAA
                     TALIALPFAVLCWNDFSFFLNFYFLGEEGRPTGGHSIWHFLWMGGWAVPSTVQLALLG
                     GAMAVAYLYPHHKGMPVWQSVTVMLMAFFIFYPKVHGGYWIMLVAMLSVWAVQDRKVL
                     LRTFLAFVPMILATMFARGEVKAPPVEFYGSWFLGFLLALLGLLLFVDAVRLAMRQSP
                     FTDGDQAQSSRLPASGPERQS"
     gene            complement(1448..>1898)
                     /locus_tag="PLJ11_07650"
     CDS             complement(1448..>1898)
                     /locus_tag="PLJ11_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOO04577.1"
                     /translation="RTPPRAYYHSCPCLSMVALTPEMQELFKATKHFAFATASKDGEP
                     NVVPIGMAIMMDPETIWIGDQFFKRTLANVKENPRVALLVWTSGAPGCLKLKGDVTLV
                     QKGPDYERMYRMVKERKPNLECKGLLIMKVTEVYDCRSGPDAGKRLL"
BASE COUNT          305 a          625 c          571 g          397 t
ORIGIN      
        1 tgatcgggct ggccctgggc ttcgaccccg aaggatatca ggggctgcac cgcttcatcc
       61 ttccgctcgc cgccctcaag ggaatgacga taactgaggt gccgatcgtg cactacgccc
      121 ggccctccgg cagctcgtac atcaagttct acaccgtgcc gttcatcacc ctccgggact
      181 actccaagtt caagaaggac cacaaggacg agatcgcaac cctacgcggc aaggggcggt
      241 aggggatagg gatgtcgttg gaagggcgat ggcagcggct ccatgcctgg ctgagggcgc
      301 tctcccccgc gaggaagctg gccttctatg ccatcatcac cctgttggtc tatggcacca
      361 cgatcgtggt ggacaacgcc gtcctcgtgt ctatgttgga cgaggacctc tcccatccgg
      421 acctgggcgt ctaccgtgcc cgggcccaga ccatcctgga tgggggactg ctctacaggg
      481 acgtgcacac cgagaccccg ccggtcatca actacctcct ggtgcccccc cagctgctgg
      541 gaggggcgga gcacgactgg gtgtggagca tctacttctc cctgttcgcc ttcctgctgg
      601 ccgccctgat gtacctcgga ctgcgcccct tcgacgagga caaggcctac ctcgccgggc
      661 tgctggtcct gctctccccc tataccatcg tggagtcggg cacgggggag gacgagtcca
      721 tcgtcgcctt cgtcttcatg ctctccgtcg tacttatgca actgaggaaa aaacaccttg
      781 ccagcgcctc catcggggtg ggggtctgga ccaagatgtt ccccatcctg ctcttcccgg
      841 tccatttcct gcagcaacgc aactggaggg accgcatcct ctccgtggcc atagtggggg
      901 cggccaccgc cctcatcgcc cttcccttcg cggtcctgtg ctggaacgac ttctccttct
      961 tcctcaattt ctacttcctc ggggaggagg gcagacccac cggggggcac agcatttggc
     1021 acttcctctg gatgggcgga tgggcggtgc ccagcaccgt gcagctggcg ctcttgggcg
     1081 gggccatggc cgtcgcctac ctctatcccc accacaaggg gatgccggtc tggcagagcg
     1141 tgaccgtcat gctcatggcc ttcttcatct tctaccctaa ggtccatggg gggtactgga
     1201 tcatgctggt ggccatgctg tccgtgtggg cggtgcagga ccgcaaggtg ctgctcagga
     1261 ccttcctggc cttcgtcccg atgatcctgg ccacgatgtt cgccaggggc gaggtcaagg
     1321 ccccgcccgt ggagttctac ggcagctggt tcctcggctt ccttctggcc ctgctcggcc
     1381 ttctcctctt cgtggacgcc gtccggcttg ccatgcggca gagccccttc accgatgggg
     1441 accaggctca gagcagccgc ttgcctgcat cgggcccgga gcggcagtcg tagacctcgg
     1501 tgaccttcat tatcagcagg cccttgcatt ccaggttggg cttgcgttcc ttgaccatcc
     1561 ggtacatcct ctcgtagtca gggcccttct ggacgagggt gacgtccccc ttgagcttca
     1621 ggcacccagg cgcgccggag gtccaaacga gcagcgccac cctggggttc tccttcacat
     1681 tggccaaggt cctcttgaag aactggtccc cgatccagat ggtctcgggg tccatcatta
     1741 tcgccatgcc gatggggacc acgttcggct ccccgtcctt ggaggcggtg gcgaaggcga
     1801 agtgcttagt ggccttgaaa agctcctgca tctcaggtgt cagagcgacc atggacaggc
     1861 atggacatga atgataataa gcccttggag gggtccgg
//
LOCUS       DAOSYN010000250         1892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_45769_length_1892_cov_4.550354, whole genome shotgun sequence.
ACCESSION   DAOSYN010000250 DAOSYN010000000
VERSION     DAOSYN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45769_length_1892_cov_4.550354"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLJ11_07655"
     CDS             complement(<1..925)
                     /locus_tag="PLJ11_07655"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOO04578.1"
                     /translation="MKKEMTAFDVAAIASELQAVRGGFLDKIFHWDGRNVLLRINVQG
                     EGRKELMLKDGRWLHLAAERPETPDTPSGFAVHLRKVLYNSRITAVSQREFDRIITLD
                     LTNREGSYQLIFELIGDGNLIVVHEGRIVNALEQKRWRHRDVVIGAEYSYPPSRFDPR
                     TASAEEFERAVLTSRSDLVRTLATSVNLGGQYGEEVCLRTGMDKGRKASGLTAEELSA
                     LRTAILGLFRELEAPMAQLVLDGEEAVDATPVPLKVNEGFVSQGMPSLSAALEAFLAQ
                     RRGEVEEDAELQRLQRQLEQQLRGVEAMEREA"
     gene            1018..1092
                     /locus_tag="PLJ11_07660"
     tRNA            1018..1092
                     /locus_tag="PLJ11_07660"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:Thr,seq:ggt)
     gene            complement(1244..1411)
                     /locus_tag="PLJ11_07665"
     CDS             complement(1244..1411)
                     /locus_tag="PLJ11_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04579.1"
                     /translation="MRSNRCLDPKRYFPQYLSMSFRSSFVRGGAFGRLSLEGLGAHLP
                     DDTGMSTAAPF"
     gene            1687..>1892
                     /locus_tag="PLJ11_07670"
     CDS             1687..>1892
                     /locus_tag="PLJ11_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04580.1"
                     /translation="MDASISFNSFICEVNTDRGSEFFSDRPKDERRTRNSAVSKNGFQ
                     PFLEANGVRHAVSRVNDPQTNGKL"
BASE COUNT          343 a          617 c          597 g          335 t
ORIGIN      
        1 ccgcctcccg ctccatggcc tcgacgcccc tgagctgctg ctccagctgc cgctgcagcc
       61 gctggagctc ggcgtcctct tcgacctccc cccttcgctg tgccaggaac gcctcgaggg
      121 cggcggagag cgaaggcatc ccctgggaca cgaacccctc gttcaccttg agcggtacgg
      181 gggtggcgtc caccgcctcc tccccgtcca ggacgagctg ggccataggg gcctcgagtt
      241 ctcggaagag gccgaggatg gcggtgcgca gggcggacag ctcctccgcc gtaaggccgg
      301 aggccttcct gcccttgtcc atcccggtcc tcaggcacac ctcctccccg tactgtcccc
      361 cgaggttgac ggaggtggcc agggtgcgca ccaggtcgga ccgggaggtg aggacggccc
      421 gctcgaactc ctccgcgctg gcggtgcggg ggtcgaagcg cgacggaggg tacgagtact
      481 ccgcgccgat gaccacgtcg cggtgccgcc accgcttctg ctccagggcg ttgactatcc
      541 tcccctcatg caccacgatg aggttcccgt ccccgatgag ttcgaagatg agctgatagc
      601 tcccctccct gttcgtcagg tccagggtga tgatgcggtc gaactcccgc tgcgacaccg
      661 ccgttatgcg ggagttgtac agcaccttgc ggaggtggac cgcgaagccc gagggcgtgt
      721 cgggggtctc aggccgctcg gcggccaggt gcagccaccg cccatccttg agcatcagct
      781 ccttcctccc ctccccctgg acgttgatcc tgagcagcac gttccgtccg tcccaatgga
      841 atatcttgtc caggaagcct ccgcgcaccg cctgcagctc cgaggcgatg gccgccacat
      901 cgaacgccgt catctccttc ttcatcgccc cctctagcat ccccccaatc aaaagggttt
      961 tgccctattc cagtggcaaa aggtttatga acgacgaccg ggctgtgggt gaagcaggcc
     1021 actgtggctt agaggtatag cgacgccttg gtaaggcgta ggtcgagggt tcgatcccct
     1081 ccagtggctc catccccttc tctgtagagc acagaaatta tttcggtacg atagtttcat
     1141 catcgtgacg aagctgaacg atacgaagat acgcttcatc tgcaggcaga tctccgcggg
     1201 aacgatgagc tgccggcagg tagtggcctc tatggcgtca gcgtcagaag ggtgcagcag
     1261 ttgacatgcc agtatcgtcg gggaggtgag cccccaagcc ctccaaggag aggcgcccga
     1321 aggcgcctcc cctcacgaac gacgagcgga agctcataga taggtactgg gggaagtacc
     1381 gcttcgggtc caggcatcta ttcgacctca tgcgctctga gggggtccag atgcctcatc
     1441 ccaaggcgcg ggcgcgcctt gtgcggaccg gacgcgtgct ggacgcccga acaagcggaa
     1501 gaagcgcggc cgatgccgtt acgagcggga gcgttccttc tcgctcatac acggagactg
     1561 gcacaggacc tcggaagagc acccccacgt catcctgtgg caggacgacg tcagtcggat
     1621 ggtcctcgcc gggggcgagc atccgagcgg tcgagggagc attccatcga cacgctgagg
     1681 caggcgatgg atgcgtcaat atcgttcaac tcgttcatct gcgaggtgaa caccgaccgg
     1741 gggtcggagt tcttctccga tcgtccgaag gatgagagga ggaccaggaa cagcgccgtt
     1801 tccaagaacg gcttccagcc gttcctggaa gcgaacggcg tacggcacgc ggtcagccgg
     1861 gtgaacgacc cgcagaccaa cgggaagctg ga
//
LOCUS       DAOSYN010000251         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_45939_length_1886_cov_4.176406, whole genome shotgun sequence.
ACCESSION   DAOSYN010000251 DAOSYN010000000
VERSION     DAOSYN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45939_length_1886_cov_4.176406"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLJ11_07675"
     CDS             <1..495
                     /locus_tag="PLJ11_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenobyrinic acid a,c-diamide synthase
                     (glutamine-hydrolyzing)"
                     /protein_id="HOO04581.1"
                     /translation="RLPEADLYYLGGGYPEAHLEELASNRDFLEGLRTAAEDGKAIYA
                     ECGGLMVLCSAIQAGERRDMAGVFPYVAELSSRRQGLSYVRARGTAESPFFAGWEVRG
                     HEFHYSRLVPEPPGPFGFLVKRGTGIDGRHDGLVQRKVLGCYLHQHALSRLDWGQRML
                     ETAR"
     gene            531..989
                     /locus_tag="PLJ11_07680"
     CDS             531..989
                     /locus_tag="PLJ11_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOO04582.1"
                     /translation="MPIGDPGGGEAPPQEKGWLTGVRIAAVPVRDLDVAVEFYTERVG
                     LRLRRRGGGWAELGNSEGPGFVLLYVPRPGERRPGDGSGAMLSCDSIYDLHRRLVDEG
                     VTFKLKPQRQPWGGLMAVFLDQDGNELMVLEGSDWGHSGGIRSDSINYNG"
     gene            1013..1792
                     /locus_tag="PLJ11_07685"
     CDS             1013..1792
                     /locus_tag="PLJ11_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04583.1"
                     /translation="MLEIENLTVEVAGKEILHNVSLSVKSGYTTVLFGPNGSGKSTLL
                     MTIMGFSGYKVRSGRILLNGEDITHLPVNERAKRGIGMMMQRPPNITGVRLKDLVRVA
                     SKGKVDIAKIAEELDMTNFLERDVNVGFSGGEIKRSEILQLTAQNPCLLLLDEPESGV
                     DMVSIEKLGAKVRELLQGPSHCAGWRERSGKAALIITHTGQILDYVEADRGYVMCNGT
                     VACSGNPRELLAEIRKMGYQECIRCKLNQPNLVVENVASEG"
BASE COUNT          356 a          553 c          683 g          294 t
ORIGIN      
        1 cggctgccag aggccgacct gtactacctg gggggagggt accccgaggc ccatttggag
       61 gaactcgcct ccaaccggga cttcctggaa ggcctccgga ccgccgcgga ggacggcaag
      121 gccatctacg ccgagtgcgg ggggctcatg gtgctgtgct cggccatcca ggccggggag
      181 cggagggaca tggccggggt gttcccctac gtggcggagc tctcctcccg ccgccaaggg
      241 ctctcgtacg tgcgggcacg ggggacggcc gagagtccct tcttcgccgg ctgggaggtc
      301 cgggggcatg agttccacta ctcccgcctc gtcccggaac cgcctggacc gttcggcttc
      361 ctggtcaagc gggggaccgg catagacggc cgccacgacg gcctggtgca gaggaaggtc
      421 ttaggctgct acctccacca gcatgccctg tcccgattgg actgggggca gcggatgctc
      481 gagacggcgc ggtgaagaac gcttttctat ccgcaaggcc atcatcgatc atgccgatcg
      541 gcgaccccgg agggggcgag gcgcccccgc aggagaaggg ctggctaacg ggagtgcgta
      601 tagccgccgt gcccgtgcgg gatctggacg tagccgtgga gttctatacc gaaagggtgg
      661 ggctgcggct ccgccggcgg ggcgggggat gggcggagct tgggaactcc gaggggccgg
      721 gcttcgtcct gctctatgtc ccccggccag gcgagaggcg cccgggggat gggagcgggg
      781 cgatgctctc ctgtgacagc atctacgacc tgcaccgccg gctggtggac gaaggagtga
      841 cattcaagct caagccccag cgccagccct ggggcgggct gatggcggtc ttcctggacc
      901 aggacggaaa tgaactcatg gtccttgaag gctcggactg ggggcattcc ggtggtatta
      961 ggtccgatag cattaattac aatgggtaac aatcgctgag ggagatgagc ccatgctgga
     1021 gatagagaac cttaccgtgg aggtcgcagg caaggagatc ctgcacaacg tgtccctgag
     1081 cgtgaagagc ggatacacca ccgtattgtt cgggcccaac ggctccggga aatccacctt
     1141 gctgatgacc atcatggggt tctccgggta caaggtgagg agcggccgca tcctgctgaa
     1201 cggggaggat atcacccatc tgccggtgaa cgagcgggcc aagcggggca ttggcatgat
     1261 gatgcagcgc cccccgaaca tcacgggggt gaggctgaag gacctggtcc gcgtagccag
     1321 caagggcaag gtggacatcg ccaagatcgc cgaggagctg gacatgacca acttcctgga
     1381 gcgggacgtc aacgtcggct tctccggcgg ggagatcaag cgctcggaga tcctccagct
     1441 caccgcccag aacccgtgcc tgctgctgtt ggacgagcct gagagcggcg tggacatggt
     1501 ctccatcgaa aagctcgggg ccaaggtgcg ggagctgctc cagggcccat cgcactgcgc
     1561 cggatggagg gagaggagcg gcaaggccgc gctcatcatc acccacacag gccagatatt
     1621 ggactacgtg gaggcggacc gcggctacgt catgtgcaac ggcactgtgg cctgctccgg
     1681 caacccccgc gagctcctgg cggagatccg caagatgggg tatcaggagt gcatccgctg
     1741 caagctcaac cagcctaatc tggtcgtaga gaacgtcgcg tccgagggat gagcatggcc
     1801 agcgacgagg agcggagggc gagggaagcc ctgaggaagg cggcgaggtt cggcgacgac
     1861 atcgacctgg aggcgtacag cgaggg
//
LOCUS       DAOSYN010000252         1867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_46565_length_1867_cov_2.682671, whole genome shotgun sequence.
ACCESSION   DAOSYN010000252 DAOSYN010000000
VERSION     DAOSYN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46565_length_1867_cov_2.682671"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLJ11_07690"
     CDS             complement(<1..675)
                     /locus_tag="PLJ11_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOO04584.1"
                     /translation="MNARFGEYGGQYVPEVLVPALMELEREYANAMADPAFRKELNDY
                     NRHYGGRETPLYFARRLTEMCGGAKVYLKREDLCHGGAHKFNNVMGQALLARRMGKER
                     LIAETGAGQHGVATAMAAAVLGLRAEVYMGERDVARQRMNVFRMELLGAKVHEVRSGS
                     RLLKDAINEALRDWAGSVESTHYLMGTAAGPHPYPTMVRDFQSVIGREIRAQLLAAEG
                     RLPDLPV"
     gene            complement(672..1286)
                     /locus_tag="PLJ11_07695"
     CDS             complement(672..1286)
                     /locus_tag="PLJ11_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOO04585.1"
                     /translation="MTRLKVCGMRSLEDLERCHAADYLGFVVMADSPNELGMEEAAAL
                     MSSCGPRRVAVTTEARPERLRGLVRALDPDVLQLHTPKYPEALRAAGDLGVPVWGMMA
                     IGPGTVGPPSLEGIEALVLDSPGPRAGGNGRPHDWARSREVREALAPFPVVLAGGLGP
                     GNAALAIRTVRPYALDVSSGVSGEEGKDPEKVRELIAAMRGALE"
     gene            complement(1283..>1867)
                     /locus_tag="PLJ11_07700"
     CDS             complement(1283..>1867)
                     /locus_tag="PLJ11_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04586.1"
                     /translation="PGEPVAMIGISLGGAAALSVLTEPRYFRGSLRYLEMAVRTGLPV
                     LMKDFVVDDRQVEAAARAGASAVLLIEGAFDRGMAFGRDGLISHAHGLGLEVVLETSD
                     LQEFEAARASEADILAYNRRDLRTFRPGPDLLEEALGALRADGRPSLAMSLMASAEDV
                     RRARDLGASGVLVGSALSTAPCPRDRLRALQVGR"
BASE COUNT          264 a          747 c          567 g          289 t
ORIGIN      
        1 cacgggcagg tccgggagcc ggccctctgc tgccagcaac tgcgccctga tctcccggcc
       61 gatgacgctc tggaagtccc gcaccatggt ggggtaggga tggggcccgg cggccgtgcc
      121 catcaggtaa tgggtgctct cgacgctccc cgcccaatcg cgcagcgcct cgtttatggc
      181 atctttgagc agccgggagc cggaccgcac ctcatgcacc ttggccccca ggagctccat
      241 gcggaagacg ttcatgcgct gcctcgccac gtcccgctcg cccatgtaca cctccgcccg
      301 gaggccgagc acggcggcgg ccatggcggt ggccacccca tgctgccccg cccccgtctc
      361 cgcgatgagc cgctccttcc ccatgcgcct ggccaggagc gcctgcccca tcacgttgtt
      421 gaacttgtgc gccccgccgt ggcagaggtc ctcccgcttc aggtagacct tcgccccgcc
      481 gcacatctcc gtgaggcgcc gggcgaagta aaggggggtc tccctccctc cgtaatgccg
      541 gttgtaatcg ttcagctcct tcctgaaggc cgggtccgcc atggcgttcg cgtattccct
      601 ctccagctcc atcagggcgg gcaccaggac ctctggcacg tactgccctc cgtactcccc
      661 gaaccgggcg ttcattccaa tgcacctctc atcgctgcga tcagctctct taccttctcg
      721 gggtccttgc cctcctcccc gctcactccg ctggaaacgt ccagcgcata gggccgcacc
      781 gtccttatgg ccagggcggc gttccctggc cccaggcccc cggccaggac caccgggaac
      841 ggcgccaggg cctcgcgcac ctccctgctc ctggcccagt cgtgcggtct cccgttcccc
      901 cccgcccgcg ggccggggga gtccagcacc agggcctcga tgccctccag ggacggcggc
      961 ccgacggtcc ccgggcctat ggccatcatt ccccacaccg gtaccccgag gtccccggcg
     1021 gccctgagtg cctctggata tttaggtgta tgcagttgta gcacgtccgg gtccagcgcc
     1081 cgcaccagcc cgcgcagcct ctcaggccgg gcctccgtgg tcaccgccac tcgccgcggg
     1141 ccgcagctgg acatcagggc ggccgcctcc tccataccca gctcgttggg ggaatcggcc
     1201 atcaccacga acccgaggta gtccgccgca tggcaccgct ccaggtcctc gagggaccgc
     1261 atcccgcaga ccttgagcct ggtcatcggc ccacctggag cgctctcagc cggtcgcggg
     1321 ggcagggcgc cgtgcttagg gcggagccga cgagcacacc cgaggccccc aggtccctcg
     1381 ccctgcgcac gtcctccgcg ctggccatca ggctcatcgc gagcgagggg cggccgtccg
     1441 ccctcagggc gcccaacgcc tcctccagca ggtccggccc cggccggaag gtgcggaggt
     1501 cccggcggtt gtacgccagg atatcggcct cggaggcgcg cgccgcttcg aactcctgaa
     1561 gatcggacgt ctccaagacg acctccaggc caagcccatg agcgtgggag atcagcccat
     1621 cccttccgaa ggccattccc cggtcgaagg ccccttcgat gagcaggacc gccgacgccc
     1681 cggcccgcgc cgcggcctcc acctgccggt cgtcgaccac gaagtccttc atcagcacgg
     1741 gcagccctgt cctgaccgcc atctccagat agcgcaggga cccgcggaag taccggggct
     1801 ccgtcagcac cgagagcgcc gcggccccgc cgagcgagat accgatcatc gcgaccggct
     1861 cccctgg
//
LOCUS       DAOSYN010000253         1861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_46767_length_1861_cov_2.864341, whole genome shotgun sequence.
ACCESSION   DAOSYN010000253 DAOSYN010000000
VERSION     DAOSYN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46767_length_1861_cov_2.864341"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1141
                     /locus_tag="PLJ11_07705"
     CDS             197..1141
                     /locus_tag="PLJ11_07705"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO04587.1"
                     /translation="MRAVVTGAAGFIGSTLTDRLLAEGHEVVAVDNFDDYYSGKMRFL
                     RRHLENERFRLLEIDILDLDALRKSFEGADVVFHMAAQAGVRISVRDPLKAHHANTTG
                     TLHVLLAARDAGVRRVINSSSSSVYGNAVRLPAREEDPTVPISPYAASKLAAEHYCRL
                     FHSLYGLDSVSLRYFTVYGPRQRPDMAIRTFTDRALDGKRPQIYGDGEQTRDFTFITD
                     VVDAIMRCADCPDLKGAPLNICSGSTVSVNQVVHAILKTVGREDITPEHLPPQPGDVD
                     HTWGDNTRARELLEWEPRVGIEEGLARFIEWYRTVGRA"
     gene            complement(1138..1734)
                     /locus_tag="PLJ11_07710"
     CDS             complement(1138..1734)
                     /locus_tag="PLJ11_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04588.1"
                     /translation="MKAYIRVIYSSEGGSPWKVDAVLQGKGFKKVPGVPGYEIDVLNE
                     TELAQVLEGLHEALRGLGVRYVPTLGAMEGQPATGYRQRLARLRELNLDPVDLGGLLE
                     EDVEAFKAAVHEGVGTFLDTIIAERQEEVAEAEAKLRAERSRAVLMEQLREGRTFHEL
                     LKAVPMEEEELSQRLLELVEKGAIRAEQRGRSVVYVAL"
BASE COUNT          309 a          615 c          601 g          336 t
ORIGIN      
        1 ccccagggtc ctaatggtcg cgcccatgcc tatatcacct ggaggcgatg atgtgggccg
       61 tcttatttaa ggtttgttag cggttctata aaatggcttg ggaatgcggc cgccccttcc
      121 catcgccatg gaggcgttgg agaggcggtg aggtccaagc aatcattaat acggcgtaga
      181 cattccccga agcagcatga gggctgtggt caccggggcc gccgggttca ttgggagcac
      241 gctcaccgac cgcttgttgg ccgaggggca cgaggtggtg gcggtcgaca atttcgatga
      301 ttactactct ggcaagatgc gcttcctccg tcggcacctg gagaacgaac gcttccggct
      361 gctcgagatc gacatcctgg acctggacgc cctccgcaag agcttcgagg gcgcggacgt
      421 ggtcttccac atggccgcgc aggcgggcgt ccgcatctcc gtgagggacc ccttgaaggc
      481 gcaccatgcc aacaccaccg gcaccctgca cgtgctgttg gccgcgaggg acgcgggggt
      541 gcgccgggtc atcaactcct cgtcctcctc cgtctacggg aacgcggtcc ggctgcctgc
      601 gagggaggag gaccccaccg tcccgatctc cccctacgcc gccagcaagc tcgccgccga
      661 gcattattgc aggctcttcc attcgctcta cgggctggat agcgtgtccc tgcgctattt
      721 caccgtgtac ggcccgaggc agcgcccgga catggccatc cggaccttca ccgaccgggc
      781 cttggacggg aagcggccgc agatctacgg ggacggggag cagacccggg acttcacctt
      841 catcacggac gtggtcgatg cgatcatgcg gtgcgccgat tgcccggacc tcaagggcgc
      901 gccgttgaac atttgcagcg gctccaccgt gagcgtgaac caggtcgtcc atgccatcct
      961 gaaaacggtg gggagggagg acattacgcc ggagcacctt ccgccccagc ccggggacgt
     1021 ggaccatacc tggggcgata ataccagggc cagggaactc ctcgaatggg agccgagggt
     1081 ggggatcgag gaggggctgg cccgcttcat cgaatggtac aggaccgtgg ggcgtgccta
     1141 gagggctacg tacacgacgc tgcggcccct ctgctcggcg cggatcgccc ctttctcgac
     1201 cagctccaat agcctctggg agagctcctc ctcttccatc ggcacggcct tcagcagctc
     1261 gtggaaggtc cgcccctccc tcagctgctc catcaggacc gcgcgggagc gctcggcccg
     1321 gagcttggcc tcggcctcgg ccacctcctc ctgccgctcg gcgatgatgg tgtcgaggaa
     1381 cgtcccgacc ccctcatgga cggcggcctt gaacgcttcg acgtcctcct cgagcaggcc
     1441 gcccaggtcc accgggtcca ggttgagttc ccgcagccgg gcgagccgct gccggtagcc
     1501 cgttgccggc tggccctcca tcgcccccag ggtcgggacg tagcgcacgc caagccccct
     1561 cagggcctcg tgcaggccct cgagcacctg ggccagctcc gtctcgttca ggacgtcgat
     1621 ctcatagccg gggaccccgg gcaccttctt gaagcccttg ccctggagga ccgcgtccac
     1681 tttccagggg gagccgcctt cgctggagta tatgaccctg atgtacgcct tcatgccgat
     1741 cggggtaaaa tattcagggt tgtctatata gtttggtatc ccggcggatc ggcgggaacg
     1801 cccgtcacaa ctatttatcc ggcggaggcc atggggaggg gcgtgagcag agaggccgag
     1861 t
//
LOCUS       DAOSYN010000254         1847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_47188_length_1847_cov_3.154576, whole genome shotgun sequence.
ACCESSION   DAOSYN010000254 DAOSYN010000000
VERSION     DAOSYN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47188_length_1847_cov_3.154576"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PLJ11_07715"
     CDS             complement(<1..1165)
                     /locus_tag="PLJ11_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO04589.1"
                     /translation="MEEPLPPQSTGNLSDYPREYREFTWHTIEWEFDWCRGGPYNVAA
                     EAVDRHARQRPDKVAIHSVRANGEVQSLTFADVSRSSSKFADGLSRLGVRKGDRVFVY
                     LDRRSELFISIIGIAKMGAVACPLFSALGPEAVKDRLLDGAPSAIITSPYLYERISSV
                     LKEASPAPLVVCVGEGCPANAVPYPQLIGSGSPDFVAVSMRPDEPYIIHYTSGSTGKP
                     KGVMLPHRAMLQQLAGAKYVADLREDDVLWCTADPGWVTGTSMGIFGPWYLGSTIVSF
                     EGRFDARTWYSLIDRFKVSVWFTAPTALRMLMRAGDEVAREFDLTSLRHICSAGEPLN
                     PHVVRWGMEVLKRRIHDNWWQTETGAPCIANFPCMPIKPGSMGKALPGVVAAVV"
     gene            complement(1279..>1847)
                     /locus_tag="PLJ11_07720"
     CDS             complement(1279..>1847)
                     /locus_tag="PLJ11_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOO04590.1"
                     /translation="EEGVQFCTDLVRHDLGDNYWPTYDSSYYQATGGHAYQEASEEID
                     AILDLIGVSDSNDQVENLALVLSFINEHVVYSPDLDNRYSAPVETLAYGSGDCDDFAA
                     LAAALFERIGVESAIAFFANDADEGHAMNLVKLNDLGDYGYYFYGDLTGQGLDDGRWI
                     IIEPQSLIDDQYDPEWLEQWDLKVAAEI"
BASE COUNT          370 a          572 c          555 g          350 t
ORIGIN      
        1 ccaccaccgc cgccaccacc ccgggcaagg ccttgcccat cgaccccggc ttgatgggca
       61 tgcatgggaa gttggcgata cacggcgccc ccgtctcggt ctgccaccaa ttgtcgtgga
      121 tccggcgctt cagcacctcc atcccccacc ggaccacgtg agggttcagg ggttcccccg
      181 cggaacagat gtgcctgagg gacgtcaggt cgaactccct cgccacctcg tcccccgccc
      241 tcataagcat gcgaagggcg gtcggcgcgg tgaaccagac gctgaccttg aaccggtcga
      301 tcagggaata ccaggtgcgg gcgtcgaagc ggccctcaaa ggagacgatc gtgcttccga
      361 ggtaccaagg cccgaatatg cccatggacg tgcctgtcac ccacccaggg tcggccgtgc
      421 accagaggac atcatcctcg cgaaggtccg ccacatactt cgcgccggcg agctgctgca
      481 gcatggcccg atgcgggagc atgaccccct tgggcttgcc ggtggaaccg gaggtgtaat
      541 ggattatgta gggctcgtcc ggcctcatgc tcaccgcgac gaagtccggc gatcccgatc
      601 cgatgagctg tgggtatggt acggcgttcg caggacaccc ttccccgacg cagaccacca
      661 ggggtgcggg ggaggcctcc ttgagcacac tggatatcct ttcgtagaga tagggcgagg
      721 taatgatggc cgacggggcg ccatcaagca gacggtcctt aacggcctcg gggccaagag
      781 cggagaacag cggacaggcc accgcgccca tcttcgctat gccgatgatg gagatgaaga
      841 gttcggagcg tcggtccagg tatacaaaca cacggtcccc cttcctcaca cctaacctgc
      901 tcaggccgtc cgcgaacttg ctggatgaac gtgagacgtc cgcgaaggtg agggactgga
      961 cctcaccgtt ggcccgcacg gaatggatcg cgaccttgtc ggggcgctgt ctggcatgac
     1021 ggtccaccgc ttccgccgcc acgttgtagg gaccgccgcg acaccaatcg aactcccatt
     1081 cgatggtgtg ccaggtgaat tccctgtatt cccgagggta atcggaaagg ttgcccgtgc
     1141 tctgcggagg caatggctct tccatatcag ttctcctggg aatgccaggt ccaacggcga
     1201 tagattattt aaaaggatac agaaaagatg gcggggaatc ggaaaaaagg agtgtagtcc
     1261 ggacaattgt ccggtttttc agatttcagc cgccaccttg aggtcccatt gctccagcca
     1321 ctccggatcg tattggtcat cgatgaggga ttgcggttcg atgatgatcc atctgccgtc
     1381 gtccaatccc tgtccggtca ggtcaccgta aaaatagtac ccgtaatcgc ccaggtcgtt
     1441 cagtttcacc aggttcatgg catgcccctc gtccgcgtcg ttagcgaaga aggcgatggc
     1501 gctctccacg cctatcctct cgaagagggc ggccgccagg gcggcgaagt catcgcaatc
     1561 gccagaccca taggccaagg tctccaccgg ggcgctgtat cggttgtcca ggtccgggga
     1621 gtatactacg tgctcgttaa tgaaggacag caccaaggcc aagttctcca cctggtcgtt
     1681 cgaatcggag acgccgatga gatcaaggat agcatcgatc tcctccgacg cctcctggta
     1741 ggcgtggcct ccggtggcct ggtagtagct ggagtcatag gtcggccaat agttatcgcc
     1801 caggtcgtgc ctcaccaggt ccgtacagaa ctggacgccc tcctcgt
//
LOCUS       DAOSYN010000255         1838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_47493_length_1838_cov_2.551318, whole genome shotgun sequence.
ACCESSION   DAOSYN010000255 DAOSYN010000000
VERSION     DAOSYN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47493_length_1838_cov_2.551318"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLJ11_07725"
     CDS             complement(<1..188)
                     /locus_tag="PLJ11_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04591.1"
                     /translation="MTGIRDTIPLSELMYGFYGSDMLDGLFAKTDYPVLTTTTGVHNA
                     IFGPKVMLIALMPAALQNS"
     gene            complement(214..1446)
                     /locus_tag="PLJ11_07730"
     CDS             complement(214..1446)
                     /locus_tag="PLJ11_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04592.1"
                     /translation="MFQSNSELPVNVRRALPDAEQSIFREAYNRTGSLDQAWRAVEPS
                     DNVRWFTCYASVQQVDSAGEMFSIDGLANAMPDYLARGGPMFFEHRAYEIGTAYRGES
                     VIHERKGLPAIKLYCAAYLDCRIADRAWAKIKSGEFPDVSIGGDALSQYWKCDDRSCY
                     NYIDRVEWFDTSPTRKGANPGSSIIDVNMKAKSEQEAPVMVEACPCEDKEKMTEGPPT
                     EGTPAPAPDDAGLVAKSDDPQVVEQLLQKVLAMFSEIKNVMNNLVASQKAPEGQAIED
                     AVDGGEMPKEAAEGGGQEEVPPAQEQAEEKPKEGEPEPMKEEAEPEAAPPKEEDEMPD
                     DEKMKQKAQEMAEAKVAEVKEMKEKAEAAVMTPRPGMAQQPPEMFAKHTLDWSDPATQ
                     RRIARTPARDLIRGILKD"
     gene            complement(1536..>1838)
                     /locus_tag="PLJ11_07735"
     CDS             complement(1536..>1838)
                     /locus_tag="PLJ11_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04593.1"
                     /translation="RKRKLKESEARDMAEAIAWKIRLHGQEPNPWVRPAVDDARSKAA
                     ALFKRNGTRAIADYVITRAQEYIDRKGIADTGKMGQTFTLTDITTGEVLENHGLGE"
BASE COUNT          244 a          611 c          523 g          460 t
ORIGIN      
        1 gagttctgca aggccgccgg catcaaggca atgagcatca ccttcggccc gaagatggca
       61 ttgtgaacgc cggtggtggt cgtgagcacg gggtagtcgg tcttggcgaa cagcccgtca
      121 agcatgtcgg agccgtagaa cccgtacatc agctccgaaa gcggtatggt gtctctgatc
      181 cctgtcattt ctggtattcc ttctcctgta agttcaatcc ttaagtatgc ccctgatgag
      241 gtctctggcc ggggtgcgtg cgatcctccg ctgcgtggcg gggtcgctcc agtcgagcgt
      301 gtgcttggcg aacatctccg gcggctgctg tgccatccct ggcctggggg tcatgacggc
      361 ggcctccgcc ttctccttca tctccttgac ctccgcgacc ttcgcttcgg ccatctcctg
      421 cgctttctgc ttcatcttct catcgtcggg catttcatct tcctctttcg gtggcgcggc
      481 ctccggctcg gcctcctctt tcataggctc aggttcgcct tccttcggct tttcctcggc
      541 ctgttcctgc gcgggcggta cttcctcttg cccgccccct tccgccgctt ccttgggcat
      601 ctctcccccg tccacggcgt cctcgatggc ctggccctcc ggggctttct gcgaagccac
      661 caggttgttc atcacgttct ttatctcaga gaacatggcc agaaccttct gcaacagctg
      721 ttcgacgacc tgcgggtcgt ccgatttcgc caccaggccc gcatcatcgg gcgctggtgc
      781 tggcgtgccc tcagtgggcg gtccttctgt catcttttcc ttgtcctcgc acgggcaggc
      841 ttccaccatc acgggggcct cctgctctga tttagctttc atattgacgt ctatgatcga
      901 gcttccgggg ttcgccccct tccttgtggg ggaggtgtcg aaccactcca cccggtcgat
      961 gtaattgtaa cagctccggt catcgcactt ccaatactgg ctcagtgcgt ccccgccgat
     1021 gctgacgtcg gggaactccc cgctctttat cttggcccac gcccggtccg ctatgcggca
     1081 gtccaggtat gcggcgcagt agagcttgat ggccggcagc cccttgcgct catgtatgac
     1141 cgactcccca cggtacgccg tgccgatctc gtaggcgcgg tgctcgaaga acatgggacc
     1201 gcccctggcc aggtagtcgg gcatggcatt ggccaggccg tctatggaga acatctcccc
     1261 ggcgctgtcc acctgctgga cgctggcgta gcaggtgaac caccgcacgt tgtccgaggg
     1321 ctccacggcc ctccatgcct ggtccagcga gccggtcctg ttgtatgcct cgcggaagat
     1381 gctctgctcc gcgtccggga gggcgcgccg cacgttgacc gggagttcgc tattggattg
     1441 gaacatctaa cttagtggcc ctaccgtgta ctgaatcgag gcgttgttgt cgctctcgac
     1501 ggcgatctcc ttcctttcaa gttcaatgag cattatcatt ctcctagtcc gtggttctcc
     1561 agcacctcgc cggtggtgat gtctgtaagc gtgaacgtct ggcccatctt gccggtgtcc
     1621 gctattccct tgcggtcgat gtactcctgg gcgcgggtga tgacgtagtc ggcaatggcc
     1681 cgcgtgccgt tgcgcttgaa cagcgcggcg gccttgctcc gggcatcgtc taccgccggc
     1741 ctcacccaag ggttcggctc ctgtccgtgg aggcgtatct tccacgctat ggcctcggcc
     1801 atgtccctgg cctcgctctc ctttagcttc ctcttgcg
//
LOCUS       DAOSYN010000256         1836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_47569_length_1836_cov_2.555867, whole genome shotgun sequence.
ACCESSION   DAOSYN010000256 DAOSYN010000000
VERSION     DAOSYN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47569_length_1836_cov_2.555867"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /gene="pheT"
                     /locus_tag="PLJ11_07740"
     CDS             complement(<1..1384)
                     /gene="pheT"
                     /locus_tag="PLJ11_07740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOO04594.1"
                     /translation="MPVINFNLEDLKALVGTKMEDREVLERIPMIGADFHEFDPVTKE
                     TAIEFFPNRPDLYSVEGLARALRSFFDIEQGLRTYDMASSDIVLKVDPSVKAVRPYVV
                     GAVLRGVALDDKGIRSLMELQEKLHLTVGRRRAKVAIGVHDLDKVRPPFVYKAVPPDS
                     LSFVPLAEDEAMTLAEVLERHEKGRDYRHLLEGKPLYPVILDHNNDVLSFPPIINGRL
                     TTVTTETKNLFIDVTGTDQNTIGGVLNIVCTSLAERGGVIETIALRGEEKGISPNLRP
                     KQWLLDVDRTNASLGLDLEPLAMCQCMTRMGYSAEPKGRKVRVWSSPVRMDLLHIADV
                     VEDVAIGYGYENFGNSKPTASTIGGERRIERVTDLVRQMLVGFGYWEVTTLTLSSLEE
                     QFSKVPFPEVEAVEVLNPVSEDHNCLRVHLVPSLLTVLRKSKHRDLPQRIFEVGDVVI
                     EAKRRRHLAVM"
     gene            complement(1447..1713)
                     /locus_tag="PLJ11_07745"
     CDS             complement(1447..1713)
                     /locus_tag="PLJ11_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04595.1"
                     /translation="MDGVRYQDLLELADNMAPEEIDYVMRGVAMNKAIAEYGLASESL
                     SGLGAGKAKKLAEIAAALVLAGELSTLAAQAAGELGKAHKALGR"
BASE COUNT          320 a          600 c          545 g          371 t
ORIGIN      
        1 ccatgaccgc caggtgccgt cggcgcttcg cttcgatgac cacgtcgccc acctcgaaga
       61 tgcgctgcgg caggtcccgg tgcttgctct tcctcaggac ggtcagtagg gacgggacga
      121 gatggacccg gaggcagttg tggtcctcgc tcacggggtt caacacctcc accgcctcga
      181 cctcggggaa cggcaccttg gagaactgct cctcgaggga ggagagcgtc agggtggtca
      241 cctcccagta gccgaaccca accagcatct ggcggacgag gtccgtcacc ctctcgatcc
      301 tgcgctcgcc gcctattgtg ctggcggtag gcttggaatt gccgaagttc tcgtacccgt
      361 acccgatggc cacatcctcg accacgtccg caatgtggag caggtccatc cgcaccgggg
      421 aggaccagac cctgaccttc cttcccttcg gttcggcgga atagcccatc ctggtcatgc
      481 actggcacat ggccaggggc tcaaggtcca gccccaggga ggcgttggtc cggtcgacgt
      541 ccagcagcca ctgcttgggg cgcaggttgg gggagatgcc cttctcctcc ccgcggaggg
      601 cgatggtctc gatgaccccg cccctctcgg ccagggaggt gcacactatg ttcagcactc
      661 cgcctatggt gttctggtcc gtgccggtga catcgatgaa aaggttcttg gtttcggtgg
      721 tgaccgtggt cagcctgccg ttgatgatcg gcgggaacga cagcacatcg ttgttatgat
      781 caaggatgac cggatagagg ggtttccctt ccagaaggtg cctgtaatcc cttcccttct
      841 catgcctctc cagcacctcc gcgagggtca tggcctcgtc ctcggcaaga ggaacgaagg
      901 aaagggagtc gggagggacg gccttgtaga cgaaaggggg gcgcaccttg tccaagtcgt
      961 gcacgccgat ggccaccttg gccctcctgc gccccacggt cagatgcagc ttctcctgca
     1021 gctccatcag ggaacgtatg cccttgtcat ccagggcgac gcctcggagg accgctccga
     1081 ccacgtacgg gcgcaccgcc ttgaccgacg gatcgacctt cagcacgatg tccgaggacg
     1141 ccatgtcgta cgtgcgaagc ccctgctcga tgtcgaagaa cgaacggagc gccctagcca
     1201 gcccttccac cgagtacagg tccggacggt tgggaaagaa ctcgatggcc gtttccttgg
     1261 tgaccgggtc gaactcatgg aagtcggcgc cgatcatcgg gatccgctcg aggacctcac
     1321 ggtcctccat cttggtcccc accaaggcct tcaggtcctc gaggttgaag ttgatgactg
     1381 gcatgcggcc gcaccatgcg gttgaaggta tatgagggtt tttaatttgc gcttagctcg
     1441 ggaacgttat cttcccaagg ccttatgggc cttgcccagc tcgcccgcgg cctgggccgc
     1501 cagggtcgag agttcccccg ctagcactag ggcggcggcg atctccgcca gcttcttggc
     1561 cttgcccgcc cccaggcctg acaatgattc cgatgccaac ccgtactcgg cgatggcctt
     1621 gttcatggcc actccgcgca tcacatagtc aatttcctcg ggcgccatgt tgtcagccag
     1681 ttcgagtaag tcctggtacc tgacaccgtc catgatatcg cggtcacagt cccgagcgcc
     1741 ctcgccgccc tccgcagcct gaccctgacg gcccttgcga aacactgttt taccatcaac
     1801 gcttacactg actatgttag tgtgctcgcc ctcgat
//
LOCUS       DAOSYN010000257         1809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_48538_length_1809_cov_3.506842, whole genome shotgun sequence.
ACCESSION   DAOSYN010000257 DAOSYN010000000
VERSION     DAOSYN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48538_length_1809_cov_3.506842"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PLJ11_07750"
     CDS             <1..964
                     /locus_tag="PLJ11_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO04596.1"
                     /translation="ALFLSSYIFGRASDVHGRKLYLQSGLVLTSIALFLLVFADSKAS
                     IAVVRILIGLCAGIYPSALLTYVYEMEGKIGKFTAYGSLGVAIGNFVAGMIAIYYGIF
                     LMSALMLLVAYFISTKLTFGNERVHKVPLFPATMIRHNFPIYVSIMFRHTGANMIWVV
                     YPLFLADLGASPLFIGLIYAINSGAQYAFMRFIDRFQTYHLIMAGFVFSIITFPSYTL
                     ATRPEEILPMQVSLAAAWSCLYVGSIKYLMERNEEKGTSSGLLQSFLSVSAIIGALVG
                     GACVFALGYHGTMFIATSLAIIGFLIFIIGNRSLDRKEKGVIAS"
     gene            980..>1809
                     /gene="rnz"
                     /locus_tag="PLJ11_07755"
     CDS             980..>1809
                     /gene="rnz"
                     /locus_tag="PLJ11_07755"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOO04597.1"
                     /translation="MDITFLGTGGSVPTTKRSTVAIALRIGPEVILFDCGEGTQRQLM
                     ASSVSFMRISRVFLTHHHGDHFLGLPGLIQSMNFYGRSAPLDVYGPSGTAKMTKAMLE
                     LGIFDLRYEVDGHDLTPGDEVRGEGHSVTAFQAEHTVPALGYVFQEDQRPGRFDVERA
                     LALGVVEGPSFARLVEGGNVKGRGGIVTPAMVMGPPRKGIRVVYSGDTAPCQGLERAC
                     QGADVLVHEATVASELEDKAREFGHSTARDAAELAKRAGVASLYLVHISGRYEDPSPL
                     L"
BASE COUNT          302 a          589 c          564 g          354 t
ORIGIN      
        1 ggcgctcttc ctgtcctcgt acatcttcgg gcgggcctcc gacgtccatg gccgcaagct
       61 ctacctgcag tccggcctgg tgctcaccag catcgccctc ttcctgctcg tgttcgcgga
      121 cagcaaggcc tcgatcgccg tggtgcgcat cctcatcggg ctctgcgcgg gcatctatcc
      181 gtccgcgctg ctgacctacg tctatgagat ggaagggaag atcggcaagt tcaccgccta
      241 tggcagcctg ggggttgcca tcggcaactt cgtcgccggg atgatcgcca tctactacgg
      301 gatcttcctg atgagcgccc tgatgctgct tgtggcctat ttcatctcca ccaagctgac
      361 cttcggcaac gagcgcgtgc acaaggtccc gcttttcccg gccacgatga tcaggcacaa
      421 tttcccgata tacgtctcta tcatgttccg gcacaccggg gccaacatga tctgggtagt
      481 atacccgctc ttcctggccg acctgggggc cagccccctc ttcatcgggc tcatctatgc
      541 gatcaactcg ggcgcgcagt acgccttcat gcgcttcatc gaccgcttcc agacctacca
      601 tctgatcatg gccggcttcg tcttctccat catcacgttc ccgtcctaca ccctcgcgac
      661 caggccggag gagatcctcc ccatgcaggt gtccctcgcc gccgcctggt cctgtctgta
      721 cgtaggctcg ataaagtacc tgatggagag gaacgaggag aagggcacct cgtccgggct
      781 gctccagtcc ttcctcagcg tctcggccat catcggcgcc ctcgtgggcg gggcatgcgt
      841 ctttgcactt gggtaccatg gcacgatgtt catcgcgacg agcctggcga tcatcggctt
      901 cctgatattc atcatcggga accgctcctt ggaccggaag gagaaagggg taatagcctc
      961 ctagggggtt agggtgcgca tggacatcac tttccttggg acggggggca gcgtcccgac
     1021 cacgaaacgg agcaccgtgg ccatcgccct gcgcataggg ccggaggtca tcctcttcga
     1081 ttgcggggag gggacgcaga ggcagctcat ggcctcctcg gtgtccttca tgcgcatctc
     1141 ccgcgtgttc ctcacccatc accacggcga ccatttccta ggcctccctg gcctcattca
     1201 gtcaatgaac ttctatggcc gctccgcgcc gctggacgtg tacgggcctt cgggaacggc
     1261 caagatgacc aaggccatgc tggagttggg gatcttcgac ctgaggtacg aggtggacgg
     1321 gcacgacctc acccctggag acgaggtgag gggcgaaggc cattccgtca cggcgttcca
     1381 ggcggagcat accgtccctg ccctgggcta cgtattccag gaggaccagc ggccagggcg
     1441 cttcgatgtc gaacgggcgc tggcgttggg ggtggtggaa ggccccagct tcgcgcgcct
     1501 cgtggaggga gggaacgtca agggaagggg gggcatcgtg accccggcca tggtcatggg
     1561 cccgccgcgc aagggcatcc gggtcgtgta cagcggcgac accgccccct gccagggatt
     1621 ggagcgcgca tgccaaggcg cggacgttct ggtccacgag gccacggtcg cctcggagct
     1681 ggaggacaag gcaagggagt tcgggcacag caccgcccgg gacgcggccg agctggcgaa
     1741 gagggcgggc gtagcctcgc tctatctggt gcacatcagc gggaggtatg aggacccctc
     1801 cccccttct
//
LOCUS       DAOSYN010000258         1790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_49185_length_1790_cov_4.246686, whole genome shotgun sequence.
ACCESSION   DAOSYN010000258 DAOSYN010000000
VERSION     DAOSYN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49185_length_1790_cov_4.246686"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLJ11_07760"
     CDS             complement(<1..453)
                     /locus_tag="PLJ11_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HOO04598.1"
                     /translation="MELHIDRDAEGSALFRFDPRVKLVCIFALIVLVSMVTDASALAF
                     ALLFAVLLLAFSRVPPVQLARGYLAALPFIALSSLTMLLASGPGSAMAMALRVSTSVL
                     LLLLLVRTTPFMDLLWALRWFKVPALLTDMIMLTYRFIFVLLDELERMR"
     gene            complement(499..789)
                     /locus_tag="PLJ11_07765"
     CDS             complement(499..789)
                     /locus_tag="PLJ11_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HOO04599.1"
                     /translation="MSRRGCLKAAMVVLLLFAIGLVGYFALSAGSPDGLERVMEDNGV
                     EEGDQLWSAPLDYGGDWGTAFVAGLIGFALTFGAVYLYLKVYLKGGSARRKA"
     gene            complement(786..1454)
                     /locus_tag="PLJ11_07770"
     CDS             complement(786..1454)
                     /locus_tag="PLJ11_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOO04600.1"
                     /translation="MGGTATHIPDGLMDPWVVLLGWALAIPVIAMAIYRLDKRVDERT
                     VPFMAILAAGIFVAQMLNFPVGGGTTGHLIGATLAVVLLGLNGAIVVMTAILVIQCLV
                     FGDGGLTALGLNLLNMAVIAPLVAYGVLRALKGHEGAAVPVASWASVFLASLACGLML
                     SASYVISDGAFGIIGSVAIPAMAGWHAVIAIGEAVITSGVVLFLKRVAPEMLEMSGPE
                     EAGA"
     gene            complement(1511..>1790)
                     /locus_tag="PLJ11_07775"
     CDS             complement(1511..>1790)
                     /locus_tag="PLJ11_07775"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOO04601.1"
                     /translation="VTRTGVTGGGGFAADQLEGMVRRGREIDPSMPLAAGFGISRPDQ
                     VREARALGVDGVIVGSALVALALSGAGAEEVREKVRQLAEAGREGTMQ"
BASE COUNT          350 a          612 c          566 g          262 t
ORIGIN      
        1 gcgcatcctc tccaattcat ccagcagcac gaagatgaac ctgtaggtga gcatgatcat
       61 gtcggtgagc agggcgggca ccttgaacca gcgcagcgcc cagagcaggt ccatgaaggg
      121 ggtggtgcgc accaggagga ggagcaggag gacggaggtg gacaccctca atgccatggc
      181 catcgcgctg ccagggcccg atgcgaggag catggtaagg gaggagaggg cgatgaaggg
      241 gagggcggca aggtatccgc gggccagttg cactggtggg acgcgggaga aagctaagag
      301 cagaacagcg aacagaaggg cgaaggccag ggcggacgcg tcggtgacca ttgagacgag
      361 gacgatcagg gcgaagatgc agaccagctt caccctgggg tcgaagcgga agagggcgga
      421 gccctcggcg tcccggtcga tgtgcagctc cattgtcggt ccagccttcc tgtcacgatg
      481 ggatctgaac gacgggcatc atgccttcct tcgggcggaa ccgcccttga ggtacacctt
      541 gaggtacaga tagacggccc cgaaggtcag ggcgaagccg atgaggccgg ccacgaaggc
      601 ggtgccccaa tcgccgccgt agtccagggg ggcgctccat agctggtcgc cctcctcgac
      661 gccgttgtcc tccatcaccc tctccaaacc gtccggggat ccggcggaga gggcgaagta
      721 gccgacgagc ccgatggcga agaggagcag caccaccatc gcggccttca ggcatcccct
      781 gcggctcatg cccccgcctc ctccggcccg ctcatctcca gcatctccgg cgccacgcgc
      841 ttgaggaaca gcaccacccc gctggtgatg acggcctcgc cgatggcgat cacggcgtgc
      901 cagcctgcca tggccgggat ggcgacgcta ccgatgatgc cgaatgcccc gtcggaaatg
      961 acatagctcg cgctcagcat caggccgcac gccagggagg ccaggaacac ggaggcccag
     1021 gaggccaccg ggaccgccgc gccctcgtgg cccttcaagg cccgaagcac gccataggcc
     1081 accaggggcg cgatgaccgc catgttcagg aggttgagcc cgagggcggt cagcccgccg
     1141 tcgccgaaca ctaggcactg aatgaccagg atggccgtca tgaccacgat cgcgccgtta
     1201 aggccgagca ggaccaccgc caaggtcgcg ccgatcagat ggccggtggt ccctcccccc
     1261 accgggaagt tcagcatttg ggcgacgaag atgcccgccg caaggatcgc catgaacggc
     1321 accgtgcgct cgtccaccct cttatcgagc ctatatatgg ccatggcgat gaccgggatc
     1381 gccagggccc agccgagcag gaccacccac gggtccatca gtccatcggg tatgtgcgta
     1441 gcagtccctc ccacctatcg tgttattaat ttgaaattag taacacggta tgctggagcc
     1501 ctatatgtac ctattgcatc gtcccttcgc gtcccgcctc cgcaagctgc ctgaccttct
     1561 cccgtacctc ctccgccccc gcgccgctca aggccaaggc caccagggcg ctacccacga
     1621 tcaccccgtc cacgccgagg gcccgcgcct ccctcacctg gtccgggcgc gagatcccga
     1681 agccggcggc caaaggcatc gaggggtcga tctcccttcc ccttcgcacc atcccttcca
     1741 gctggtcggc tgcgaacccc cctccgcccg tcacgcccgt cctggtgacc
//
LOCUS       DAOSYN010000259         1789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_49236_length_1789_cov_3.037486, whole genome shotgun sequence.
ACCESSION   DAOSYN010000259 DAOSYN010000000
VERSION     DAOSYN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49236_length_1789_cov_3.037486"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..925
                     /locus_tag="PLJ11_07780"
     CDS             86..925
                     /locus_tag="PLJ11_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04602.1"
                     /translation="MKYDSGHFEGGYRLMHIHRTKRNLFLVQAVLVLVFAAYLVYAGG
                     GFSARPFYLGIDSFLYFVLIMALLMMVEAYVFTVLEMRFVKSASAKFIITQRAFRSAL
                     AWTLVWVVVLMVFALPFVPEAVQDATRSRGELVAGPSDAPGQFELFNTDLFGLTEVST
                     INVRAQGAVEVFVLTEHNYLLFKDDGMDMLAVYRVNVNDHLAAPELAIDFPDMPRGRF
                     YVLFYSVSGGPVEVEYTIEHEIANSLMDYMPLMAIAFIAVNGVAAGYMLLLNRQNRQG
                     IYR"
     gene            complement(916..1725)
                     /locus_tag="PLJ11_07785"
     CDS             complement(916..1725)
                     /locus_tag="PLJ11_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04603.1"
                     /translation="MRTVAVYRPGRQFPSVSVTGARCALMCEHCQGRFLRGMAEAGSP
                     PALLSFAARLEREGGTGLLLSGGCDAQGRVPVRPFLPAIREIKATTRLLVNVHPGLVS
                     PQEGRLLADSGADRIALDLVLDPTAIAAQLHLARSPQDYIDSLDALCRAAPGRVAPHI
                     LLGLGEEGRELEAVRTAGRRGVACVVLLMLIGRKVDDWEARLLRAVREGADGPPVLLG
                     CMRPRGRPDVEIAALEAGAAGIANPSKGTVQVITGKGWALEERPVCCALHR"
BASE COUNT          279 a          606 c          598 g          306 t
ORIGIN      
        1 tcaggaagtg ggccaccctg gcctgatgcg ctcggccctt acaggaacct ttattaagtg
       61 gccagggaat tatttgtggg ttcccatgaa atatgatagc ggccatttcg agggcgggta
      121 ccgccttatg cacattcatc gcaccaagcg gaacctgttc ctcgtgcagg ccgtcctggt
      181 cctggtgttc gccgcctacc ttgtctatgc gggcgggggc ttcagcgcca gaccgttcta
      241 cctgggcatc gactcgttcc tctacttcgt gctcataatg gcgctcctaa tgatggtgga
      301 ggcctacgtg ttcaccgtcc tggagatgag gttcgtgaag agcgcgagcg ccaagttcat
      361 catcacccag cgggcgttcc gaagcgcgct ggcctggacc ctggtctggg tggtggtgct
      421 catggtgttc gccctgccgt tcgtacccga ggcggtgcag gacgccacga ggagtcgagg
      481 ggagctggtc gcgggcccgt cggacgcgcc cgggcagttc gagcttttca acacggacct
      541 cttcgggctc accgaggtct cgaccatcaa tgtccgggcg cagggagcgg tggaggtgtt
      601 cgtgctcacc gagcacaact acctgctctt caaggacgac ggcatggaca tgctggccgt
      661 gtaccgcgtg aacgtcaacg accacctggc cgcgcctgag ctcgccatcg atttcccgga
      721 catgccccgg ggcaggttct acgtgctgtt ctactcggtg agcgggggcc cggtggaggt
      781 ggagtacacc atcgagcatg agatcgccaa cagcctgatg gactacatgc cgctgatggc
      841 catcgcgttc atcgcggtca acggcgtggc cgcggggtac atgctcctgc tcaaccgtca
      901 gaacaggcag ggcatctacc ggtgaagggc gcagcagacc gggcgctcct ccagtgccca
      961 ccccttcccc gtgatcacct ggaccgtgcc cttcgacgga ttggcgatcc ccgcggcccc
     1021 agcctccagc gccgcgatct ccacgtccgg acggccccgg ggccgcatgc aaccgagcag
     1081 gaccgggggg ccgtcggccc cttcgcgcac cgcgcgaagc agccgggcct cccagtcgtc
     1141 cacttttctg ccaataagca ttaggaggac gacgcacgcc acgcccctcc ggccggcggt
     1201 gcgcaccgcc tccagctcgc gcccctcctc tcccaggccc aggaggatgt ggggcgccac
     1261 cctgccgggg gcggcccggc agagcgcgtc cagggagtcg atgtagtcct gcggggagcg
     1321 ggcgaggtgc agctgcgccg cgattgccgt ggggtccagc acaaggtcga gggcgatgcg
     1381 gtcggccccg ctgtccgcga gcagcctccc ctcctgggga gagacgagcc cggggtgcac
     1441 gttcaccagc aggcgggtcg tcgcctttat ctccctgatc gccgggagga acggcctgac
     1501 cggcaccctg ccctgcgcgt cgcacccccc gctcaggagc agccccgtcc ccccctcccg
     1561 ctccagtcgc gcggcgaagg agagcagggc cggcggggac ccggcctcgg ccatcccccg
     1621 caggaagcgc ccctggcagt gctcgcacat gagggcgcac ctggccccgg tgacggagac
     1681 ggaggggaac tgccttccgg gacggtagac ggcgacggtc ctcatggcag gggatcgatg
     1741 gcgcccgggg gctttgttct attggtcggt ggatgacatc ccacggaac
//
LOCUS       DAOSYN010000260         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_49760_length_1775_cov_2.345349, whole genome shotgun sequence.
ACCESSION   DAOSYN010000260 DAOSYN010000000
VERSION     DAOSYN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49760_length_1775_cov_2.345349"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PLJ11_07790"
     CDS             <1..1089
                     /locus_tag="PLJ11_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04604.1"
                     /translation="RNVLKLLNMGFLQIIRPFADVLAIMLRPIAMFFRIVGREVMKRV
                     REKRKELKAQGYSGIALGGALMAELPSIYLDVLMEQLGKIDWGTFFSKILNVLWENLT
                     VRLPAFLGKVFEILNGLGKWIWENIILAIGNLALTELAKFGWWLWTEITEAIGDMAIA
                     IGTFGVWLWTEITDGIGNLGSALSGFGNWLWKSITQGIGNVGSILGGFGDWVYKVLTG
                     AITLPQALLDIGTTFYDMFVKVYNGFADFANMFSLFTGITVQKVLTSAEKNAWSNASH
                     QSEKDRFYANNPQFKSMAVGGLMTSDGLIYAHAGEAVIGTDSLRGMIADAISVNNSNT
                     IVIPGSVIADAIDRRVDYYYKYKVANRR"
     gene            complement(1172..>1368)
                     /locus_tag="PLJ11_07795"
     CDS             complement(1172..>1368)
                     /locus_tag="PLJ11_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04605.1"
                     /translation="RDCESSYWLPVSSAATFCIQLRTVDHDEDVYLPLTVTVRDAPLS
                     SQNSCSLAGVSGQPIGEHGF"
     assembly_gap    1369..1378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1524..>1775
                     /locus_tag="PLJ11_07800"
     CDS             1524..>1775
                     /locus_tag="PLJ11_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04606.1"
                     /translation="MTYSWTYATAVYIDNTNVTDALLSYSLSGNQTSTIVSGEIVFSK
                     RALDAILTPVTNMSVIISRIYQVTEDGVTTTTDITIFRGH"
BASE COUNT          405 a          482 c          500 g          378 t
ORIGIN      
        1 cgtaacgtcc tgaagctcct taacatgggc ttcctgcaga taatacgccc gttcgctgac
       61 gttctggcca tcatgctccg ccccatagcc atgttcttcc ggatagtggg cagggaggtg
      121 atgaagcggg tcagggagaa aaggaaggag ctgaaggccc aagggtatag cggaatagcc
      181 ctgggcggtg ctttgatggc agagcttcca tccatctacc ttgacgtcct catggagcag
      241 ttggggaaga tagactgggg gacgttcttc tccaagatac tcaacgtgct gtgggagaac
      301 ctcaccgtcc ggctccccgc cttcctgggc aaggtgttcg agatactgaa cggcctgggg
      361 aagtggatat gggagaacat catcctggcc atcgggaatc tggccctcac agagctggcc
      421 aagttcggat ggtggctttg gacggagata actgaggcaa ttggcgatat ggccattgca
      481 ataggcacgt tcggggtctg gctatggacg gagataaccg acggcatcgg caatcttggt
      541 tcggcgctta gtggatttgg taactggctt tggaagtcca taacccaagg gataggtaat
      601 gtaggaagca tattgggcgg cttcggggat tgggtatata aggtcctcac gggggccata
      661 acgctccctc aggcattgct ggacatcggg acaacgttct atgacatgtt cgtgaaggtg
      721 tacaacggct tcgccgactt cgcaaatatg ttcagcctgt tcactggaat aacggtccag
      781 aaggtgctta catctgctga aaagaacgcg tggtccaacg cttcccatca atcagagaag
      841 gataggttct acgccaataa tcctcagttc aagagcatgg cggttggcgg cctgatgacc
      901 agcgatggcc tgatctacgc ccacgcgggc gaggcggtca tcgggacaga ttcgcttagg
      961 ggcatgatag ccgacgccat ttccgtgaac aattccaata ccatcgtcat tccaggctcg
     1021 gtcattgccg atgccattga cagacgggtg gactattatt acaagtacaa ggtggcgaac
     1081 cgcagatgag cttttcagga tacatttcta catcaaccaa ctatgcatca gggtacagcc
     1141 tcggctacat cgaaaagctg tccctctcgt atcagaaccc atgttccccg atgggctggc
     1201 cagacactcc ggccagcgag caggagttct gtgatgacag cggggcatcc ctcaccgtca
     1261 ccgtgagcgg caagtacacg tcctcatcat ggtccacggt cctgagctgg atgcagaacg
     1321 ttgccgcgct gctgaccggt agccagtacg atgattcgca atcccgctnn nnnnnnnnat
     1381 ctggccgtct atgaggggag cacctacgtg tttccctccc agggcatcca tgtcatagtg
     1441 gagtcgttcg agcctaccat aagcgagggg acgcagcccc tcatcgagta caccctcaag
     1501 gtgaaccagc aggtgagcta ctaatgacct actcctggac atatgccacg gcggtgtaca
     1561 tcgacaacac taacgtcacc gacgccctgc tgtcgtattc gctcagcggc aaccagacgt
     1621 cgaccatcgt gtccggcgag atagtgttct ccaagcgggc gctggacgcg atactgacac
     1681 cggtcaccaa catgtcggtc atcatatccc gtatctacca ggtgaccgag gacggggtca
     1741 ccaccaccac cgacatcacc atctttcgcg gccac
//
LOCUS       DAOSYN010000261         1774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_49799_length_1774_cov_2.726003, whole genome shotgun sequence.
ACCESSION   DAOSYN010000261 DAOSYN010000000
VERSION     DAOSYN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49799_length_1774_cov_2.726003"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLJ11_07805"
     CDS             complement(<1..446)
                     /locus_tag="PLJ11_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HOO04607.1"
                     /translation="MKVSIIGTGYVGLVSGVCFAELGHQVLCVDAVREKVESINAGEP
                     PIFEEGLEEMLRRHLSNGTFRATTDYGEVPGTDITFLCVGTPSLEDGSLDLRYVRRAT
                     EDLGKALRGKRGRHTVVVKSTVPPNTTRDVVGPLLAETSGKGLDEIG"
     gene            609..1433
                     /locus_tag="PLJ11_07810"
     CDS             609..1433
                     /locus_tag="PLJ11_07810"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HOO04608.1"
                     /translation="MNRLQQVQLDILQELGRVCQRHGIRYFLFSGTLLGAIRHNGFIP
                     WDDDVDVVMLRDEYERFVHLPKEEFRPNYFLQTNATDPEYHNVYAKLRRSDTTFVETY
                     TEHRRINHGVFIDIFPLDGVPEGKVAQSLFWWPISILGYTATLKEFKKEPKPYLRFPF
                     LLFKWFFPFDGASMMRAYGKLIKIIDIKSTSKVGFLTHPGFPLDHVVYEKGWFDSAVT
                     VDYEGLQFSAPVEHHKVLMHLFGDYMQAPPQEMRVSHHGATVIDTERPYKERVNEV"
     gene            1465..>1774
                     /locus_tag="PLJ11_07815"
     CDS             1465..>1774
                     /locus_tag="PLJ11_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOO04609.1"
                     /translation="MTVSAIILAGGAGERFGTEIPKQFIKLAGKPIIGHTLDTFNGNP
                     SIDGIVVVVNQGYLGQMRALVNENGWSKVRAVVPGGATRQASSYEGLKACPPDTTCVL
                     I"
BASE COUNT          381 a          533 c          484 g          376 t
ORIGIN      
        1 ccgatctcat ccaggccttt cccggaggtc tccgccagga gcggccccac aacgtcccgg
       61 gtggtgttcg gggggacggt ggacttgacc acgacggtat gcctccctct cttcccccga
      121 agggccttcc ccaggtcctc ggtcgccctc ctcacatacc ttaggtccaa ggagccgtcc
      181 tccagggagg gcgtccccac gcacaggaag gtgatgtcgg tgcccggcac ctccccgtag
      241 tcggtggtcg ccctgaaggt cccattggaa aggtgtctcc gcagcatctc ctccagcccc
      301 tcctcgaaga tcgggggctc cccggcgttg atgctctcca ccttctccct gacggcgtcg
      361 acgcagagca cctggtgccc aagctccgcg aagcacactc cagagacaag ccctacatag
      421 ccagtgccga tgatagagac cttcatcctt ccgaccaggc gtatgcaagg ccttcagtat
      481 atttaaggtc cgctcagggt tcccctagga actcctgcta ttcccatgga ctacgtcctt
      541 tctccttaga tccatggcct aaaacctata aattgagaga tgtccttcaa gggtaccgtt
      601 gatggccgtt gaacagactg cagcaggtcc agctcgatat tcttcaggag ttggggaggg
      661 tatgccaaag gcacggcata cgatactttc tcttctctgg cacgctcctg ggggcgatca
      721 ggcataatgg tttcatcccc tgggacgacg atgtggacgt agtgatgttg agggacgagt
      781 atgagcgctt cgtccatttg cccaaggagg agttcaggcc caattacttc ctgcagacaa
      841 acgcaaccga ccccgagtac cataatgtct acgccaagct gcgccgctct gataccacgt
      901 tcgtagagac atacacggag catcggcgca tcaatcacgg tgtattcatc gatatcttcc
      961 ccctagacgg agttccagag ggtaaagtgg cgcagagcct cttctggtgg cccatctcca
     1021 tacttggata cacggctaca ttgaaggagt tcaagaagga gcccaagccc tatctgcgct
     1081 tccccttcct gctgttcaag tggttcttcc catttgatgg ggctagcatg atgagggcat
     1141 atggaaagct tatcaaaatt atcgatatca agagcacctc caaggtggga ttcctcactc
     1201 atcctggctt tccattggac catgtggtgt atgagaaggg ctggttcgat tcggcggtga
     1261 cggtggacta cgagggcctt cagttctcag ccccggtcga gcaccacaag gtgctgatgc
     1321 acctcttcgg cgactacatg caagcgcccc ctcaggagat gagggtttcc catcatgggg
     1381 caacggtcat cgacaccgaa cggccctaca aagaaagggt caatgaggtt taataagctg
     1441 gaatcggatt gacccgcatc gaccatgacc gtttctgcaa taatactcgc agggggcgcc
     1501 ggagagcgct tcggcactga gatcccgaaa cagttcatca agctggccgg caagccaatc
     1561 atcggccaca ccctagatac gttcaacggc aacccttcca tcgacgggat cgtggtggtg
     1621 gtcaaccaag ggtacctcgg gcagatgagg gcccttgtga acgagaacgg atggagcaag
     1681 gtccgtgcgg tggtccctgg gggcgccacc cgacaggcat cctcttacga agggcttaag
     1741 gcatgcccac ccgacacgac ctgcgtgctc atcc
//
LOCUS       DAOSYN010000262         1766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_50061_length_1766_cov_2.900058, whole genome shotgun sequence.
ACCESSION   DAOSYN010000262 DAOSYN010000000
VERSION     DAOSYN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50061_length_1766_cov_2.900058"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLJ11_07820"
     CDS             complement(<1..414)
                     /locus_tag="PLJ11_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO04610.1"
                     /translation="MEDASYDANSIQVLEGLEAVRKRPGMYIGSTDARGLHHLVYEIV
                     DNAIDEVMGGFCTRIDITINRDGSVTVADDGRGIPTGLVPKYERPAVEMVLTVLHAGG
                     KFDRKSYKVSGGLHGVGMSVVNALSVLLELEIKRDG"
     gene            684..>1766
                     /gene="thiC"
                     /locus_tag="PLJ11_07825"
     CDS             684..>1766
                     /gene="thiC"
                     /locus_tag="PLJ11_07825"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOO04611.1"
                     /translation="MFQARGGASEEIVRAARAEGADPEWVRRVVATGRAVVPFNPLHS
                     PEPCAIGEGLTVKVNANVGTSRDLPDLGPELEKARIAVRYGADTLMDLSTGGDIDEVR
                     RAILKETRVPVGTVPIYQEGLRAARRSAVVDMDEDDMFNGIERHAKDGVDFMTVHCGI
                     TLESVAHLRRAERLTDVVSRGGAFLTAWVLHNEQENPLYENFDYLLELAQRYEFVLSL
                     GDGLRPGCVADATDRAQVQELIVLGELVQRCREAGVQCMVEGPGHVPLSQIEANVLLE
                     KRVCDSAPFYVLGPLVTDIAPGYDHIVGAIGGAVAALAGADFLCYVTPAEHLSLPDGE
                     DVKEGVIASKIAAHAADLALGRGVERD"
BASE COUNT          308 a          559 c          593 g          306 t
ORIGIN      
        1 cccgtcgcgc ttgatctcga gctcgagcag caccgagagg gcgttgacca ccgacatgcc
       61 cacgccatgg agccccccag agaccttgta gctcttgcga tcgaacttgc ctccggcatg
      121 gagcacggtg agcaccatct ccaccgccgg cctctcgtac ttggggacca gccccgtcgg
      181 gatgccccgg ccgtcgtcgg ccaccgtcac gctcccatcc ctgttgatgg tgatgtcgat
      241 gcgcgtgcag aagcccccca tgacctcgtc gatggcattg tccactatct catagaccag
      301 gtgatggagc cctctggcat cggtgctccc tatgtacatt ccgggccgct tccgaacggc
      361 ctccaggcct tccagcacct gtatgctgtt cgcgtcgtag ctcgcgtcct ccatgctcct
      421 taccctcttg agatcgatgg tggtgcctct gttcgatgtc gatacctagg gttcgaccgc
      481 atcccatcct ctgattgggg aagagcgtct tactattaat accctggctt tagcatctgg
      541 ctatataagc aaacgcacaa atttttcacc tttggttcac gctgttaaca gatgatcccg
      601 gcgaagggaa aggtacatat ccggcggggg ccgagaggga agcaataaat caggaaaacg
      661 ggttggtggc ggcgatgagc atcatgttcc aagcgagggg cggggcctcg gaggagatag
      721 taagggcggc cagggccgag ggggcggacc ccgagtgggt caggcgcgtg gtggccacgg
      781 gccgagccgt cgttccgttc aacccgttgc actcgcccga gccctgcgcc atcggggagg
      841 ggctcaccgt caaggtgaac gccaatgtgg gcacctcccg ggaccttccg gacctggggc
      901 cggagctgga gaaggcccgc atcgccgtca ggtacggcgc ggacacgctc atggacctca
      961 gcacgggcgg ggacatcgac gaggtccgaa gggccatcct caaggagacg agggtgccgg
     1021 tgggcaccgt tcccatctac caggagggcc ttcgggcagc ccgccgcagc gccgtggtcg
     1081 acatggacga ggacgacatg ttcaacggca ttgagcgcca cgccaaggat ggcgtcgact
     1141 tcatgaccgt gcactgcggg atcacgctgg agtccgtggc ccacctccgc agggcggaga
     1201 ggctgaccga cgtcgtgtcc cgcggagggg ccttccttac cgcctgggtc ctgcacaacg
     1261 agcaggagaa cccgctgtac gagaacttcg attacctgct ggaactcgcg cagaggtacg
     1321 agttcgtcct gagcctgggg gacggcctgc gccctggatg cgtggcggac gccaccgatc
     1381 gggcgcaggt gcaagagctc atagtcctgg gggagctggt acagcgctgc cgggaggccg
     1441 gcgtgcagtg catggtcgag gggcccgggc acgttcccct ttcccagata gaggcgaacg
     1501 tgctcctgga gaagcgggtc tgcgacagcg cgccgttcta cgtcctgggg ccgctggtca
     1561 cggacatcgc ccccgggtac gatcacatag taggggcgat cggcggggcg gtggccgccc
     1621 tcgccggggc ggacttcctc tgctacgtca cccccgccga gcatctctcg ctcccggacg
     1681 gggaggacgt caaggaaggg gtgatcgcca gcaagatcgc ggcgcacgcc gcggacctcg
     1741 cgctggggcg cggggtggag cgcgac
//
LOCUS       DAOSYN010000263         1765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_50084_length_1765_cov_5.186550, whole genome shotgun sequence.
ACCESSION   DAOSYN010000263 DAOSYN010000000
VERSION     DAOSYN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50084_length_1765_cov_5.186550"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLJ11_07830"
     CDS             <1..313
                     /locus_tag="PLJ11_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO04612.1"
                     /translation="KVTDGLKEGGGVIANYPDDNAKLMRAVGRSENVHAVNATKIAVE
                     EIGRPMTNTAMLGALVKVTNVVNLDTIIEEMRDKMSGKLGKDAVERNIKAIRRAYEEV
                     Q"
     gene            314..598
                     /locus_tag="PLJ11_07835"
     CDS             314..598
                     /locus_tag="PLJ11_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO04613.1"
                     /translation="MADEGSSSLPLGGMIIEPGNSRRYRTGEWRSQRPEVDKEKCTNC
                     LTCWIYCPDNSVLVENEKMMGFKYSHCKGCGICANQCPAKAITMREEGVE"
     gene            595..1758
                     /gene="porA"
                     /locus_tag="PLJ11_07840"
     CDS             595..1758
                     /gene="porA"
                     /locus_tag="PLJ11_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOO04614.1"
                     /translation="MSKPIAVNGDTAIAMAWKQINPDVVAAYPITPQTIIVEAYSDYV
                     ADGLVDTEYICAESEHSALSICVGSSAAGARVATATASAGLAFMWEMLYVSASMRTPI
                     VMAVANRTLSGPINIHCDHSDAMGARDSGWLQIFGENVQEAYDNTLMAFRIAENMKVR
                     LPIMTCLDGFIITHALERFEPLEDEKAKAFVGPYQPVRPLLDVKSPTTYGPIAMPEYY
                     YEIKYQLVTAMANAMDVARQVMAEYGELTGRRYDLVEGYRTEDAEYLMVAMGSTAGTV
                     RFVVDQLRAEGKKVGAVKVRLFRPFPTEDLVALLRGKKAVAVLDRAMSPGTGAPLYMD
                     VVEAASRIGSPPRISNYIYGLGGRDIMPEQLSSVFGEIIEGRGERVNYLGVRK"
BASE COUNT          388 a          481 c          605 g          291 t
ORIGIN      
        1 caaggtgacg gatggcctca aggagggagg gggcgtcatc gccaactatc cggatgacaa
       61 cgccaagctg atgcgcgctg tggggcggag cgagaacgtt catgcggtga acgcgaccaa
      121 gatcgccgtc gaggagatcg gccgaccgat gacgaacacc gccatgcttg gagcgttggt
      181 caaggtcacc aacgtggtca acctagatac gatcatcgag gagatgaggg acaagatgtc
      241 cggaaagctt ggcaaggacg cggtggagag gaacatcaag gccataaggc gggcctatga
      301 ggaggtgcag tgaatggcgg acgagggatc gagctcatta cccttaggcg ggatgataat
      361 cgagcctggc aattccagga ggtacaggac gggggagtgg cgctcgcagc ggcccgaggt
      421 ggacaaggaa aaatgcacca attgcctgac gtgctggatc tactgcccgg ataacagcgt
      481 tctggtggag aatgagaaga tgatggggtt caagtattcg cattgcaaag gatgcggcat
      541 ctgcgccaac cagtgcccgg ccaaggccat aacgatgagg gaggagggag tggaatgagc
      601 aagcccatcg ccgttaacgg ggacacggcc attgccatgg cctggaaaca gatcaacccg
      661 gacgtggtgg ccgcctaccc tatcacccct cagaccatca tcgtggaggc gtactcggac
      721 tacgtggccg atggcctggt ggacacggag tacatatgcg cggagtcgga gcacagcgcc
      781 ctgagcatct gtgtcggctc gtccgcggcc ggggcccgcg tggccacggc cacggcctcc
      841 gccgggctcg cgttcatgtg ggagatgctg tatgtctccg cctccatgag gaccccgatc
      901 gtcatggcgg tggccaatcg aaccctgagc ggccccatca acatccactg cgaccacagc
      961 gatgccatgg gagcccggga ctccggatgg ctacagatct tcggggagaa cgtgcaggag
     1021 gcctatgaca ataccctgat ggccttccgc atagcggaga acatgaaggt ccgtctgccc
     1081 ataatgacct gcctcgatgg gttcatcatc acccacgcct tggaacggtt cgagcccctt
     1141 gaggacgaga aggcgaaggc cttcgtcggg ccataccaac cggtgaggcc actgctggac
     1201 gtcaagagcc ccacgaccta tgggcccata gccatgcccg agtactacta tgagataaag
     1261 taccagctgg tgaccgccat ggcgaacgcc atggatgtcg ctcgccaggt gatggccgag
     1321 tacggagagc tcaccggccg caggtatgat ctggtcgagg gctatcgcac cgaggacgcc
     1381 gagtatctga tggtggccat gggctccacc gcgggcaccg tccgctttgt ggtggaccag
     1441 ctcagggcgg aggggaagaa ggtgggggcg gtgaaggtcc gcctgttccg cccgttccct
     1501 accgaggacc tggtcgcgct cttgagggga aagaaggctg tggcggtgct ggacagggcc
     1561 atgagccccg ggacaggcgc cccgctttac atggacgtcg tggaggcggc gtcccggatc
     1621 gggagtccgc cgcgcataag caactatatc tatggacttg gaggaaggga catcatgcca
     1681 gagcagttga gctcggtgtt cggggagatc atcgagggca ggggagagcg cgtcaactac
     1741 ctgggggtga ggaaatgaac ctacg
//
LOCUS       DAOSYN010000264         1753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_50527_length_1753_cov_8.095406, whole genome shotgun sequence.
ACCESSION   DAOSYN010000264 DAOSYN010000000
VERSION     DAOSYN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50527_length_1753_cov_8.095406"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..628)
                     /locus_tag="PLJ11_07845"
     CDS             complement(212..628)
                     /locus_tag="PLJ11_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOO04615.1"
                     /translation="MKGRRIRVIVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     PESIVETAIQEDADAIGVSILSGAHEHYFREIIRLLKEKNAEDIILFGGGVIPEGDIP
                     SLTGMGVGAVFGPGTPTSTCIEWLENKVREKRAGES"
     gene            complement(658..>1753)
                     /locus_tag="PLJ11_07850"
     CDS             complement(658..>1753)
                     /locus_tag="PLJ11_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOO04616.1"
                     /translation="NDILKEYIARGTYIFPPKSSMRLITDIFGFCSDNVPKWNTISIS
                     GYHIREAGSTASQEVAFTLADGMAYVQAAVAAGLDPDVFGQRLSFFFNAHNDFLEEVA
                     KFRAARRLWARLMKERVGVTEPKALMLRFHTQTAGSTLTAQQPENNIVRVAIQALASV
                     MGGTQSLHTNSFDEALALPTEKSVGIALKTQQIIACESGATSTVDPLAGSYAIEALTN
                     EIERQALEYIDKIEEMGGMLSAIERGYPQQQIQDAAYQYQRSVERGERVVVGVNKFQV
                     DEESGARELLRVDPSVGDDQVKALKAMKQGRDNVLVGDRLEAVRAAARDEGVNLMPAI
                     IDAVKVYATEGEICGVLREEFGEYRENIVL"
BASE COUNT          261 a          567 c          548 g          377 t
ORIGIN      
        1 ccacctcggt gtcgccgagg ggcaggaagg ccgtcctgac cttctgctcc gccacttctt
       61 cgacccccgt gcacttgatc cccagcgatt gctcccagaa cttgagagct tcgtcgaggt
      121 tggcaacggc tatgccgata tgatctatcg ctgttggctt catgctgtcc ctcctatatg
      181 gggcgaggcc ggccctctcc cggttttcac cctaggattc cccggccctc ttctcccgga
      241 ctttgttctc tagccactct atgcaggtgc tggtgggggt cccggggccg aagacagccc
      301 cgacgcccat cccggtcaag ctgggtatat caccctcggg gatgacgccc ccgccgaaaa
      361 ggatgatgtc ctcggcgttc ttctccttca atagccggat tatctcacgg aaatagtgct
      421 cgtgggcgcc gctcaggatg ctcaccccga tggcgtcggc gtcttcctgg atggcggtct
      481 ccacgatgct ctccggggtc tgccttagcc cggtgtagat gacctccatg ccggcgtccc
      541 tgagagccct ggccaccacc ttggcccccc tgtcatggcc gtcgaggccg ggcttggcca
      601 cgataacacg gatcctccgt cccttcattt cccgtccctc ccgacaccag gcgtccccta
      661 gaggacgata ttctccctgt attcgccgaa ctcctcgcga aggacgccgc aaatctcgcc
      721 ctccgttgcg tagaccttca ccgcatcgat gatggccggc atgaggttga ccccctcgtc
      781 gcgggcggcg gccctgaccg cttcgaggcg gtcgccgacg aggacgttgt cccgcccctg
      841 cttcatggcc ttcagggcct tcacctggtc atcgccgacg gaggggtcaa ccctgaggag
      901 ttcacgggcg ccgctttcct catcgacctg gaacttgttg acgcccacca cgacgcgttc
      961 gcctctctcg acggacctct ggtactggta ggccgcgtcc tggatctgct gctgggggta
     1021 gcccctctcg atggccgaga gcatgccgcc catttcttcg atcttgtcga tgtattccag
     1081 ggcctggcgc tctatctcgt tcgtgagggc ctcgatggcg tagctcccgg cgagggggtc
     1141 caccgtcgag gtggccccgg attcgcaggc gatgatttgc tgcgtcttga gcgctatgcc
     1201 cacgctcttt tccgtgggga gggcgagggc ctcgtcgaag ctgttggtgt ggagagactg
     1261 ggtccccccc atgacggagg ccagggcctg gatcgctacg cggacgatgt tgttctcggg
     1321 ctgctgggcc gtgagggtgc tccccgccgt ctgggtgtgg aagcggagca tgagcgcctt
     1381 aggctcggtg acgccgacgc gctccttcat gagcctcgcc cagagcctcc tggcggcccg
     1441 gaatttggcc acctcctcga ggaaatcgtt gtgggcgttg aagaaaaagg aaagccgctg
     1501 gccgaagacg tcggggtcga gaccggcggc cacggccgcc tggacgtagg ccatcccgtc
     1561 ggccaaggtg aaggccacct cctgggaggc cgttgatccg gcttccctta tgtggtagcc
     1621 cgaaatggat atggtgttcc acttggggac gttgtcgctg cagaacccga aaatgtcggt
     1681 gatgaggcgc atggaggatt tgggggggaa aatgtaggtg ccgcgggcga tgtactcctt
     1741 gaggatgtcg ttc
//
LOCUS       DAOSYN010000265         1745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_50858_length_1745_cov_2.572781, whole genome shotgun sequence.
ACCESSION   DAOSYN010000265 DAOSYN010000000
VERSION     DAOSYN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50858_length_1745_cov_2.572781"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..323)
                     /locus_tag="PLJ11_07855"
     tRNA            complement(251..323)
                     /locus_tag="PLJ11_07855"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Pro,seq:cgg)
     gene            479..1186
                     /locus_tag="PLJ11_07860"
     CDS             479..1186
                     /locus_tag="PLJ11_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO04617.1"
                     /translation="MNLVRLGMREQLMEKIAGEIVLSSDPGKTLRKWREEFGISQQDL
                     ARHLSISPSVISDYESGRRRSPGIVIIRKIVTGLLDIDQQNGSKVLKKYDLGQKSDCI
                     ISIHEFRSSMPAQGFMKVIEGEVLNKGISLDKDIHGYTIIDSMKAITALGSMDYLKIY
                     GWSSERALIFTGVKFGRSPMIAIRAHPMKPALVVYQRPEHVDELAMRLATLENIPLLR
                     TDIDVPKLVERLEGLDR"
     gene            1191..>1745
                     /locus_tag="PLJ11_07865"
     CDS             1191..>1745
                     /locus_tag="PLJ11_07865"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOO04618.1"
                     /translation="MPVGIVSYGAYIPRYRIAPKEIGRVWGNDGEAMGKNLMIYSKSV
                     PSPDEDVITISVAAARNMMRRCEVDPKEIGALYVGSESHPYAVKPTGTIVAEAIEASP
                     EITVADYEFACKAGTAAIQTCMGLVGSGMIKYGVAIGADTSQGAPGDALEYSASAGGA
                     AFLIGSERIIAELNLTYSFTTDTPD"
BASE COUNT          425 a          511 c          478 g          331 t
ORIGIN      
        1 cctgatcatt ttcaggaaaa gataaaaaaa tcttttttga tattccgaat tttgaaaaat
       61 caaactcatt cgatattttt gaatatttcc gcattttaca gaaagcgtta taagggatcc
      121 acgtcaaact ttcaaaaatt ggaatagaat ctctaatcat atatatacac ccaatctaaa
      181 cagactcaaa aacacttaga taatataata gttctgcccc actataacta ttagataata
      241 aatattaaaa tgggtctgtc cggatttgaa ccggaggcta taggtcccga acctacaagt
      301 ttaccaagct aacccacaga cccgtggtag gcggttaaat cgtccctata ttaaatagct
      361 tatggaacgc tctactgaaa gcgtcccggc ctcggcctct ccatcactcc tcacgggccc
      421 attcccacgg catccacgca atgatcgtcg ataggcgtgt tcgacagtta tatataatgt
      481 gaacctagtg cgtctgggca tgcgcgagca gctcatggag aagatcgccg gggagatcgt
      541 gctctcgtcc gaccccggca agacgcttcg caaatggcga gaggagttcg gcatatccca
      601 acaggacctt gcccggcacc tctccatctc cccctcggta ataagcgact acgagtccgg
      661 caggaggagg tccccgggca tcgtgatcat ccgcaagatc gtcaccggcc tcctagacat
      721 cgaccagcag aacggctcca aagtgctcaa gaagtacgac ctggggcaga agtccgactg
      781 catcatctcc atccacgagt tccgaagcag catgcccgcg caagggttca tgaaggtcat
      841 agagggggag gtgctcaaca agggcatctc cctggacaag gacatacacg gttatacgat
      901 catagactcc atgaaggcga taacggcgct gggctccatg gactacctca agatctacgg
      961 ctggagctcc gagcgggcgc tgatcttcac gggcgtcaag ttcgggcgct ccccaatgat
     1021 cgccatccgc gcgcacccga tgaagcccgc cctggtcgtc taccagcggc cggagcacgt
     1081 ggacgagctg gccatgcgcc tcgcgacgct tgagaacatc ccgctgctca gaacggacat
     1141 cgacgtcccc aagctggtcg aaaggcttga ggggctggac aggtgatcgc atgcccgtcg
     1201 ggatagtgag ctacggcgcc tacatccccc gctaccgcat cgcccccaag gagatcggga
     1261 gggtctgggg gaacgacggg gaggcgatgg gcaagaacct aatgatctac agcaagagcg
     1321 tcccgtcccc ggacgaggac gtcataacca tcagcgtcgc ggcggcgcgc aacatgatga
     1381 ggaggtgcga ggtcgacccc aaggagatcg gggcgctgta cgtcggctcc gagtcccatc
     1441 cgtacgccgt gaagccgacg ggcaccatcg tcgccgaggc gatcgaggcc tcgccggaga
     1501 tcaccgtcgc ggattacgag ttcgcctgca aagcgggaac ggcagccatc cagacgtgca
     1561 tgggcctcgt cggctccggg atgatcaagt acggcgtggc gatcggcgcg gacacgtccc
     1621 agggggcccc cggcgacgca ttggagtact ccgcgtccgc cggcggggcg gcgttcctga
     1681 tcggctctga gaggatcata gcggagctga acctcaccta ctcgttcacc acggacaccc
     1741 ccgac
//
LOCUS       DAOSYN010000266         1741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_50992_length_1741_cov_3.457888, whole genome shotgun sequence.
ACCESSION   DAOSYN010000266 DAOSYN010000000
VERSION     DAOSYN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50992_length_1741_cov_3.457888"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLJ11_07870"
     CDS             <1..550
                     /locus_tag="PLJ11_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF835 domain-containing protein"
                     /protein_id="HOO04619.1"
                     /translation="GREASAGELVCQDCGTSLRPAAQEEEPERPKVQGLQEGCSYLLK
                     DDRPDRSYALFVEQLKKGRRGFCITRNYPVKVRSKYELGDTPMIWLSNVGKEDSLRPK
                     DLEKLNYTLEQFFSQGQGIVLLDGLEYLITNNNFLTVLRFVQSLRDQVAISNSIMLMV
                     LNPSTLDANELNLLEKEVDGVL"
     gene            complement(545..1318)
                     /locus_tag="PLJ11_07875"
     CDS             complement(545..1318)
                     /locus_tag="PLJ11_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOO04620.1"
                     /translation="MIMKGRAIFKGKAIGRLLVLDEAFSFLGGVDPRTGLLTVASGRE
                     GESIDNRILVFPHGKGSTVGSYTLMDLKKNGHLPAAIINAEAETIVATGAVMAEVPMI
                     DSIDISLLREGDRAVVNGDSLDMMDLVERKVVTCVLRRKGQILLLKRSQKVGTNRGRW
                     AGVSGFIERGERPEETALREVVEETGIATARLEGRGGTLRIRDGTYVWTIHPFLFEVG
                     DEEPRLDWEHTEFAWVEPKELSGYDTVPGFQKVLGTLGL"
     gene            complement(1315..>1741)
                     /locus_tag="PLJ11_07880"
     CDS             complement(1315..>1741)
                     /locus_tag="PLJ11_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HOO04621.1"
                     /translation="PEQPVTLEGLPVVRISRQDIEATVQRLKAGDRPDLIAIGCPHLS
                     ESEMKELAAYLDGKSRKGGTEVWFCTSRGVWLRCPKERAVLERFGKVLCDTCVVVAPI
                     EGLHRVTASDSAKAYAYLPGLCSQRALYGSQRELLEMIL"
BASE COUNT          295 a          612 c          521 g          313 t
ORIGIN      
        1 cggaagggag gcctccgccg gggagctggt ctgtcaggat tgcgggacct cgctgcgccc
       61 ggcggcgcag gaagaggagc cggagcgccc caaggtccag gggcttcagg aagggtgcag
      121 ctatctcctc aaggacgatc ggcccgaccg ctcgtacgcg ctcttcgtgg agcagttgaa
      181 gaaggggcgc cggggcttct gcatcacccg caactacccg gtcaaggtcc gcagcaagta
      241 cgagctcggg gacacgccca tgatctggct gagcaacgtc ggcaaggagg acagcctgcg
      301 cccgaaggac cttgagaagc ttaactacac gttggagcag ttcttctcgc aggggcaggg
      361 gatcgtattg ctggacggtc tggagtacct catcacgaac aacaacttcc tgaccgtgct
      421 gcggttcgtg cagtcattgc gggaccaggt ggccatcagc aactcgatca tgctgatggt
      481 gctgaacccc tccaccctcg acgccaacga gctgaacctg ctggagaagg aagtggacgg
      541 ggtcctatag gcccaaggtc ccgaggacct tttggaagcc gggcacggtg tcgtaccccg
      601 aaagctcctt cggttccacc caggcgaact cggtatgctc ccagtccagc ctgggctcct
      661 cgtcgcccac ctcgaacagg aagggatgga tcgtccatac gtacgtcccg tcccggatgc
      721 gcagcgtccc gccccttccc tccagcctcg ccgtggcgat gccggtctcc tcgaccacct
      781 cccgcagggc ggtctcctcc ggcctctccc cgcgctcgat gaagccgctc acccccgccc
      841 acctccccct gttggtgccg accttctggc tgcgcttcag gagcaggatc tggccctttc
      901 ggcggagcac gcaggtgacc accttcctct ccaccaggtc catcatgtcc agggaatccc
      961 cgttcaccac cgcccggtcc ccctcgcgca gcaacgagat gtcgatggaa tcgatcatgg
     1021 ggacctcggc catgacggcg cccgtggcca ctatcgtctc cgcctcggcg ttgatgatgg
     1081 ccgccggaag gtggccgttc ttcttgaggt ccatgagcgt gtacgagccc acggtggacc
     1141 ccttcccatg cgggaacacc aggatgcggt tgtctatgct ctccccttcc cgccctgagg
     1201 ccacggtgag cagccctgtc cgggggtcca ctcctccgag gaagctgaac gcctcgtcca
     1261 gcaccagaag ccgcccgatc gccttgccct tgaatatcgc ccgtcccttc attatcatag
     1321 tatcatctcc aacagttcgc gctgggagcc atagagcgcc cgttgcgagc acaggccggg
     1381 aaggtaggca taggccttgg cggaatcgct cgccgtgacg cgatgcagcc cctctatggg
     1441 cgcgaccacc acgcatgtgt cgcacagcac cttgccgaac ctctccagca ccgccctctc
     1501 cttcgggcag cgcagccaca cgcccctcga ggtgcagaac catacctccg tccctccctt
     1561 cctgctcttg ccgtcaaggt atgcggccag ctccttcatc tcgctttcgg agagatgggg
     1621 gcagccgatg gcgatcaggt ccgggcggtc gccggccttc agcctctgga ccgtggcctc
     1681 gatgtcctgg cgggagatgc gcaccacggg gagcccctcc agcgtcacgg gctgctcggg
     1741 c
//
LOCUS       DAOSYN010000267         1737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_51140_length_1737_cov_2.654578, whole genome shotgun sequence.
ACCESSION   DAOSYN010000267 DAOSYN010000000
VERSION     DAOSYN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51140_length_1737_cov_2.654578"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1132
                     /locus_tag="PLJ11_07885"
     CDS             158..1132
                     /locus_tag="PLJ11_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOO04622.1"
                     /translation="MTNKTLETLLRMCVSGPAERKVLAERLPNKMRAYQDGKVYEIDN
                     VREFILSEDLEGSGLVQYEIDATVRRGAVAAKCVRDVFPIINMTTDQMRIPYGNSEVV
                     APKKKEGAEFQAMYQDYNYRTLTAEVYGNFVPITEELIDDAKFDIVAIELEKMGRNLE
                     GTLNQMGITDLLDNAGKEHDTTGSNQGLTAVIKAKALCMAAGFIPDSVIMTPEAWGMV
                     FLDYKPAYNPSAEDTLRAGVMPQIVGLKPFVCSVPDASSTYTWEYNSDGDIGMLVVDA
                     VNAGVIGMRKDITVKMHEDPLKMLRAPTVSARWDFAYLQANAICRVKY"
     gene            1184..1444
                     /locus_tag="PLJ11_07890"
     CDS             1184..1444
                     /locus_tag="PLJ11_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04623.1"
                     /translation="MVLSHGNSGKVLSKTMSRMRLEYLEYPDAMTDEEKAFLESTTVN
                     NAGEANEDNWTISTIPFEWLESRGPDMVDIRKIDPLKDDMRD"
     gene            1446..>1737
                     /locus_tag="PLJ11_07895"
     CDS             1446..>1737
                     /locus_tag="PLJ11_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04624.1"
                     /translation="MTIAQLAGGKITGGEFEYRQGYKFEASDLSIDANTTWSTGSSYV
                     NVEGAGLAHVVIRAKGANASSAGTVTFNFTAVGDPTTMTTPTTTSFSQTLTVA"
BASE COUNT          439 a          441 c          519 g          338 t
ORIGIN      
        1 aacgctgtca atgaggaggc ttctggagtt gcgaccgcag ttctgcaccc gctcatcatc
       61 ggcaaggcaa tcgaggacat cgctggaggc ggtgtcggac ttgggatcgt gtgctacggc
      121 gctctgaccc aggcgaactc gagctgaggt gatggaaatg accaacaaga cattagagac
      181 cctgcttagg atgtgcgtct ccggaccggc cgagaggaag gttctggccg agaggctccc
      241 taataagatg cgggcatatc aggacgggaa ggtctacgag atcgataatg tgcgggagtt
      301 catcctctcg gaggaccttg aaggctccgg gctagtacag tatgagatcg atgcgacggt
      361 caggcgcggg gctgttgcgg cgaagtgcgt cagggacgta ttccccataa tcaacatgac
      421 cacggaccag atgcgcatcc cctatgggaa ctccgaggtc gtggccccga agaagaagga
      481 gggcgccgag ttccaggcca tgtatcagga ctacaactac cgtaccttga ccgccgaggt
      541 ctatgggaac ttcgtcccca tcaccgagga actcatcgac gacgccaagt tcgatattgt
      601 ggcgatcgag ctggagaaga tgggcaggaa ccttgagggg acgctcaacc agatgggcat
      661 taccgacctc ttggacaatg ccggtaaaga gcatgatacg accggctcca accaggggct
      721 aactgcagtc ataaaggcca aggccctatg catggcggct gggttcattc ccgactctgt
      781 cataatgacg cctgaggcct ggggtatggt attccttgac tacaagcctg cgtataatcc
      841 gagcgccgag gacactctgc gggccggagt gatgccacaa atcgtcggtc tgaagccgtt
      901 cgtctgctct gttcccgacg catcctccac ttatacctgg gagtataaca gcgatggcga
      961 cattgggatg ctcgtggtcg atgcggtcaa tgctggcgtc atcggcatga ggaaggatat
     1021 caccgtgaag atgcacgagg accccctgaa gatgctccgc gccccgaccg tgagcgccag
     1081 gtgggacttc gcgtatcttc aggcgaacgc catatgccgg gtgaaatact aaacttgaac
     1141 gggagggcaa cctcccaacc aaatcattta acaggaggat agcatggtac tgagccacgg
     1201 gaactccgga aaggtccttt caaagacgat gtcgcgcatg agattagaat atctcgaata
     1261 tccagacgcg atgactgatg aggagaaggc ctttctcgaa tcgaccaccg taaataacgc
     1321 tggagaggcc aacgaggata actggaccat aagcactatc ccgttcgagt ggctcgagtc
     1381 taggggaccg gacatggtgg atataagaaa gatcgatcca ttgaaggatg atatgaggga
     1441 ttaatatgac gatcgcgcaa ttggccggtg gaaagataac cggcggggag ttcgaatatc
     1501 ggcagggata caagttcgag gcctctgatc taagcataga tgcgaatacc acatggtcca
     1561 caggatcatc ttacgtcaat gtggaagggg cagggctggc gcatgttgtc atccgggcca
     1621 aaggagctaa tgcatcgagc gcagggaccg taacattcaa ctttacggca gtaggcgatc
     1681 cgacgaccat gaccactccc actacaacaa gcttctcgca gacgcttaca gttgcag
//
LOCUS       DAOSYN010000268         1733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_51278_length_1733_cov_4.307509, whole genome shotgun sequence.
ACCESSION   DAOSYN010000268 DAOSYN010000000
VERSION     DAOSYN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51278_length_1733_cov_4.307509"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLJ11_07900"
     CDS             complement(<1..247)
                     /locus_tag="PLJ11_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HOO04625.1"
                     /translation="MMKKMWKKNTKAVSPVIATILMVAITVVLAAVLYVMVMGFGGSD
                     TTQAPAVTLSKTTTGGGTAIVISIDRQVDIGKIGIAID"
     gene            complement(425..994)
                     /locus_tag="PLJ11_07905"
     CDS             complement(425..994)
                     /locus_tag="PLJ11_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04626.1"
                     /translation="MEEVAPQVLGRLEELGPAVSLDLNISVEAYFDTLTALIDRFCNK
                     KQLTCIYISVTVPASTIKAALGSLEIATDRLRFVDCISFATMECTPSDDAQTLFVESP
                     AMLEYIVLKMEYLFRKGRGERFMVILDSVNSLAAHNEVRMLYEFMQVLMASAKSRGAY
                     PVLLSIGEQMKPEVHEMLQLVCDQIVTLK"
     gene            complement(996..1706)
                     /locus_tag="PLJ11_07910"
     CDS             complement(996..1706)
                     /locus_tag="PLJ11_07910"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOO04627.1"
                     /translation="MAASPGAKLCTGMECIDRLVGGGLPVGGMVSVCGPCGSGKTFLA
                     MEYLLRGALAGQQGVYISTVHSPEKLLASTPRLDRAEGDPFRDGRLAIKHIDDMGSGE
                     REDGKITRPEASAIVEDIAEAIDGNKAQRLVLDATNPLMLEMQDWVARAFLVSLSKLL
                     FKTKCTGLLITEGDDMQGLEQTMADGIIRLGTAVRRGDNYRVLEVVKMAGAAHSRAKY
                     VMDMTSEGILVTPMLRGL"
BASE COUNT          292 a          579 c          505 g          357 t
ORIGIN      
        1 catcgatagc aatcccaatt ttccctatat cgacctgcct gtcgatcgag atgacgatgg
       61 cagtcccacc acccgttgtg gtcttggaca aggtcactgc cggggcctgg gtggtgtcgc
      121 tcccgccgaa gcccatgacc atcacgtaca gcacggcggc caggacgacg gtgatcgcga
      181 ccatcaggat ggtggcgatg accggggaca cggccttcgt gttcttcttc cacattttct
      241 tcatcatatg ctttcagtct cctggtaggt ttccctacca ggctctgttt gcacttctca
      301 ggataaataa ttatctatcc ggggatcata atatattcgg ttccccgatg cggcccgagg
      361 gggcggagcg ttacttacga tacttcgctc cgagctctgc gctctgccat catagtccct
      421 tgcgtcattt caaggtgact atctgatcgc agaccagctg cagcatctcg tggacctccg
      481 gcttcatctg ctccccgatg ctcagcagca ccgggtacgc gcccctgctc ttggcgctgg
      541 ccatgagcac ctgcatgaac tcgtagagca tgcggacctc gttgtgcgcg gccagcgagt
      601 taaccgagtc caggatgacc atgaaccgct cccccctccc cttgcggaag aggtactcca
      661 tcttgagcac gatgtactcg agcatggccg ggctttccac gaagagtgtc tgggcgtcgt
      721 ccgagggcgt gcattccatg gtggcgaagg agatgcaatc gacgaaccgc agcctgtcgg
      781 tcgcgatctc cagggacccg agcgccgcct tgatggtcga ggcggggacg gtcacggaga
      841 tgtagatgca cgtaagctgc tttttgttgc agaagcggtc gatcagcgcc gtcagcgtgt
      901 cgaagtacgc ctccacgctt atgttcaggt ccagggagac cgccggcccg agctcttcga
      961 gcctgccgag gacctgcggg gccacctcct ccatgtcaga gccccctgag catcggggtg
     1021 accagtatgc cctccgaggt catgtccatc acgtacttcg cgcgggaatg cgcggccccg
     1081 gccatcttga ccacctccag cacgcggtag ttgtcccccc gccgcaccgc cgtccccagc
     1141 ctgatgatgc catcggccat ggtctgctcc aatccctgca tgtcgtcccc ctcggtgatc
     1201 agcagcccgg tgcacttggt cttgaacagc agtttcgaca gggacacgag gaacgccctg
     1261 gccacccagt cctgcatctc cagcatcagg gggttggtgg cgtccagcac cagccgttgg
     1321 gccttgttcc cgtctatggc ctccgcgatg tcctcgacga tggccgaggc ctccggccgg
     1381 gtgatcttgc cgtcctccct ctcccccgag cccatgtcgt ctatgtgctt gatcgccagc
     1441 cttccgtccc tgaacgggtc gccctcggcc cggtccagcc tcggcgtgga ggccaggagc
     1501 ttctcggggc tgtgcacggt ggagatgtac acgccctgct gcccggcgag cgccccgcgg
     1561 aggaggtact ccatggcaag gaacgtcttc cccgagccgc agggcccgca gacggagacc
     1621 atcccgccca cggggagccc ccctcccacc aggcggtcta tgcactccat gccggtgcag
     1681 agcttggcgc caggggatgc agccatgcga tgatgatgcc cctatgcgga tat
//
LOCUS       DAOSYN010000269         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_51712_length_1723_cov_2.197842, whole genome shotgun sequence.
ACCESSION   DAOSYN010000269 DAOSYN010000000
VERSION     DAOSYN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51712_length_1723_cov_2.197842"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1723
                     /locus_tag="PLJ11_07915"
     CDS             <1..>1723
                     /locus_tag="PLJ11_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04628.1"
                     /translation="QCYAWPRILALFNLIYSGCRHNELYLQQYGGELFSPPSPSSPDP
                     VLRALTLFEDRLAMTDADVLELLDYIRRGGVKGASGIEAGGEAIDFSDLRTEYIGIMY
                     EGFLDFFPRLVHEEDIAYVELRGKGNHAYPLARLEEIATDRARLRDFIKKVQEGSKRA
                     DELDEAEAEEEERPEEEEEGQPEEEEGEERGEVAIDVFLARPKQPAELEARVHRWAIE
                     AAKAARLIRASASEEEQERTVASLVKNVYGPGKVFLISWKGLRKGSGTYYTRPGLVIP
                     LVRRTLEPLVLADEGTKEPRRPEEIVALKVADPAMGSGSFLVASANYLAEELYRSWKY
                     HGYMDRLANGERLLLPDGRQATGLEEEDIVALSPNGIGEERFEEYLLTRLKRHVIERC
                     IYGVDINPLAVELAKLSLWLETMDPHLPFEFLDHKLKVGNSLVGTWLFEAFDYPIAAW
                     GRYENVKKDETDPLKVALLNQNEWIKQAGKECKEELKKIAGGLSSAPLFGMDSSALSH
                     QVKDRFESVHRARERDRARLFDELTKSEDYQRLKRQMDRWCAIWFWPGKDKFREEDVP
                     FPSWFYRT"
BASE COUNT          382 a          450 c          586 g          305 t
ORIGIN      
        1 ccagtgctat gcatggccta ggatactggc cctgttcaac ctgatataca gcggctgcag
       61 gcacaacgaa ctgtacctgc agcagtacgg cggggagctt ttcagtcccc cctccccctc
      121 ttcgccggac cccgtcctcc gtgccctcac tctgttcgag gaccgtctgg caatgaccga
      181 cgccgacgtg ctggagcttc tcgactacat ccgcagggga ggggtgaagg gagcgagcgg
      241 catcgaggca ggaggggagg cgatcgattt ctccgacctt cggacggagt acatcggaat
      301 aatgtacgag ggcttcctgg acttctttcc caggctggtg cacgaggagg acatagccta
      361 cgtcgagctc aggggcaagg ggaaccacgc ctatcccctc gcccgattgg aggaaatagc
      421 gaccgatagg gcaaggctca gggactttat caagaaggtc caggagggct cgaagagggc
      481 ggatgagctt gatgaggcgg aggccgaaga ggaagagcgg cccgaagagg aggaagaagg
      541 gcagccagaa gaggaggaag gggaggagcg gggagaggtc gcgatcgatg tcttcctggc
      601 ccgtcccaag cagcccgctg agctggaggc ccgcgtgcac cgatgggcca ttgaggccgc
      661 aaaggccgcc cggctgatcc gcgcttccgc atccgaggag gagcaggaac ggacggtcgc
      721 ttcgctggtc aagaacgtgt acgggcccgg caaggtcttc ctcataagtt ggaaagggct
      781 gcgcaagggg agcggcacgt actacacaag gccggggctc gtgatcccgc tcgtgcggag
      841 gactttggag ccccttgtcc tcgctgatga gggaacgaag gaaccgagga ggcctgagga
      901 gatcgtcgcc ttgaaggtgg cggacccggc catgggcagc gggtcgttcc ttgtggcgag
      961 cgccaactat ctggcggagg agctctaccg ctcatggaag taccacggct acatggacag
     1021 gctggccaac ggcgagaggc tgttgctgcc ggacggcagg caggccaccg ggctggagga
     1081 ggaggacatc gtggccctga gccctaacgg catcggggag gagaggttcg aggagtacct
     1141 cctcacccgc ctgaagcggc atgtcatcga gaggtgcatc tacggcgttg acatcaatcc
     1201 gttggcggtg gagcttgcca agctcagcct ctggttggag acgatggacc ctcaccttcc
     1261 cttcgagttc ctggaccaca agctcaaggt gggcaacagc ctggtgggga catggctctt
     1321 tgaagccttt gattatccaa tcgccgcctg gggcagatat gagaatgtga aaaaggacga
     1381 gacagatcct ctcaaagtcg cgctgttaaa tcagaatgaa tggatcaaac aggccggaaa
     1441 ggaatgcaag gaagagctga agaagatagc aggcggcctc tcatcggcgc cgcttttcgg
     1501 catggattcg tctgcattgt ctcatcaggt caaggatcgt ttcgagagtg tgcatcgggc
     1561 gagggaaagg gacagagcgc gtctgttcga cgaactgacg aagagcgaag attatcagag
     1621 gctcaagagg caaatggacc gctggtgtgc gatttggttc tggcccggta aggataagtt
     1681 cagagaagag gacgttccat ttccatcctg gttctatcgt acg
//
LOCUS       DAOSYN010000270         1722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_51728_length_1722_cov_4.183563, whole genome shotgun sequence.
ACCESSION   DAOSYN010000270 DAOSYN010000000
VERSION     DAOSYN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51728_length_1722_cov_4.183563"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLJ11_07920"
     CDS             <1..313
                     /locus_tag="PLJ11_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion system protein E"
                     /protein_id="HOO04629.1"
                     /translation="EIVGYEPETNELITNTVYEWDPATDRFIFKGHAFLFDKIMEMRS
                     LTNDEMMAEFDRRVEVINYLVKKDVRNHREIWNIVNSYYKDSEKTLARLRADLAAMEV
                     G"
     gene            313..1353
                     /locus_tag="PLJ11_07925"
     CDS             313..1353
                     /locus_tag="PLJ11_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO04630.1"
                     /translation="MAGESDLFTRIDSTKRSYLRTKSATPAHSKEVGARVIKLPEGSV
                     PEYVKLTPYQEFCWRTIGKTVVKRMRPNPRLELALMQAHMRLRPEEYTAYVWMTTLIV
                     GILAAVLGALFGGVLIAFLGVGAGMVTVATVLMVVIPAVLTYVVLSAIPSSRAKTRMR
                     DINKRLGPAMSFISAMASADVNIDVIFKELARQPIYGEIKNEAEWITRDTDLLGTDIL
                     TAISKAAQRSPSTRFQEFLQGVVTTSTSGGQLKPYFLQKADQFEKEYKLEIKSQLETL
                     GLMAETFVTVVVAFPLFLVVIMAIMAIVPGAGGNTDMTILLLYLVVGLMIPFSQFGFI
                     FFIWNMTKESTM"
     gene            1363..>1722
                     /locus_tag="PLJ11_07930"
     CDS             1363..>1722
                     /locus_tag="PLJ11_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04631.1"
                     /translation="MADEELLDIGQLSLKKRDYSKILLILGVAGLALCCVIGLIDAIG
                     GFETGLEWIDWLAIGLMVMCGPYGFYTSYNHRKIVEIESRLPDFLRDVSEGGRFGMTL
                     ADSIKASSRGRYGHLTPE"
BASE COUNT          354 a          483 c          551 g          334 t
ORIGIN      
        1 ggagatagtc ggctacgagc cggagaccaa cgagctcatc acgaacaccg tgtacgaatg
       61 ggacccggcc accgaccggt tcatattcaa aggccatgcc ttcctgttcg acaagatcat
      121 ggagatgagg tccctgacca acgatgagat gatggcggag ttcgaccgcc gggtggaggt
      181 catcaactat ctggtcaaga aggacgtgcg caaccatcgc gagatctgga acattgtgaa
      241 ctcctactac aaggacagcg agaagaccct tgcccggctg agggcggacc tggcggccat
      301 ggaggtgggc tgatggctgg ggagagtgat ctgttcaccc gcattgactc caccaagagg
      361 tcctaccttc gcacgaagag cgccaccccc gcccactcca aggaggtggg ggcgcgtgtc
      421 atcaaactgc ccgagggaag cgtgcccgaa tatgtgaagc tcaccccgta tcaggagttc
      481 tgctggagga ccatcggcaa gaccgtggtc aaaaggatga ggccgaaccc gcggttggag
      541 ctggcattga tgcaggccca tatgcgcctg cggcccgagg agtacacggc ctatgtctgg
      601 atgaccacgc tgatcgttgg catcctcgcc gctgtgctcg gggcgctttt cggcggagtg
      661 ctgatcgcat tcctgggcgt gggggcaggc atggtgacgg tcgccaccgt gctcatggtc
      721 gtcatccccg ccgtgctgac ctatgtcgtc ctcagcgcca ttccgagttc cagggccaag
      781 accaggatgc gggacatcaa caagcgtctg gggccggcga tgagcttcat atccgcgatg
      841 gccagcgcag acgtgaacat cgacgtgatc ttcaaggagc tggcgcgcca gcccatctac
      901 ggggagataa agaacgaggc ggagtggatc accagggaca cggacctctt gggcacggac
      961 atcctgacgg cgatcagcaa ggcggcccag aggagcccct cgaccaggtt ccaggagttc
     1021 ctgcagggag tggtcaccac ctccacctcc ggggggcagc tgaagcccta tttcctgcag
     1081 aaggccgacc agttcgagaa ggaatacaag ttggagataa agtcccagct ggagacgctc
     1141 gggctcatgg cggagacgtt cgtgaccgtg gtggtggcct tccccctctt cctggtggtg
     1201 atcatggcca tcatggccat cgtcccgggg gctgggggga acacggacat gaccatcctg
     1261 ctgctttacc tggtggtggg gctgatgatc cccttctccc agttcggctt catcttcttc
     1321 atctggaaca tgaccaagga gtccaccatg tgagggcttg atatggctga cgaagagctt
     1381 ttggacatcg ggcaactgag cctcaagaag agggactaca gcaagatcct tctcattctc
     1441 ggcgtggctg gcctcgccct ttgctgcgtg atcggcctga tcgatgccat cggtgggttc
     1501 gagacgggtc tggaatggat agattggctg gccatagggc tgatggtgat gtgcggcccc
     1561 tacggcttct ataccagcta taatcaccgg aagatcgtgg agatcgagtc caggttgccg
     1621 gactttctgc gcgatgtatc ggaaggcggc cgattcggga tgacgctggc agattccatc
     1681 aaggcgtctt cccgtggcag gtacgggcat ctgacgcccg aa
//
LOCUS       DAOSYN010000271         1689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_53035_length_1689_cov_2.209302, whole genome shotgun sequence.
ACCESSION   DAOSYN010000271 DAOSYN010000000
VERSION     DAOSYN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53035_length_1689_cov_2.209302"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="cas7u"
                     /locus_tag="PLJ11_07935"
     CDS             <1..983
                     /gene="cas7u"
                     /locus_tag="PLJ11_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HOO04632.1"
                     /translation="SSSFVPASSRFATTHPRDVMVSPRQFGPFIEKRSLRKNASGPAQ
                     SVANRLEKVIIDDDGVNIRPELEGLPYVRSKLTGASEAVTSSLIEPHRLNSPFIIGNK
                     EFEKRLKEEMEYKKGANLDWRRVARVLMRYDPNSLVHGVFFSNLEDGRVRLARLLTGF
                     IEAEDIKEAYSGGVKNNSIDPSGKIRLADSQIKAGVYSNIPYHRVEYVARSIRAYFNL
                     DLSMLRSYALPPEGYDLITALSLYKVAKFLEDDLRLRTMCDLKVLGKRMVSAQEVAFP
                     SSDEVLAQLRPVIDACRDKGLFAKPPVTDLTVATKMEKEEKEGPVPEEEQ"
     gene            1003..>1689
                     /gene="csb2"
                     /locus_tag="PLJ11_07940"
     CDS             1003..>1689
                     /gene="csb2"
                     /locus_tag="PLJ11_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HOO04633.1"
                     /translation="MICIKLRLLTGRYHATPWGRNVNEGVAEWPPSPFRLLRAIYATW
                     KRQFSELDEATVDRILTALASERPEYRLPPAKSSHLRSYLHSNLSNPEKRFSGRQKVF
                     DQFVVVMPGHEITMTWPNASLDEGDLKALRELLGAMNYLGRSESWCEIGLGEPIAPSN
                     CWPDAGGPLEQGGSRIRVAGVAAPADSEGSRDRWWDTLALDSNDLARLGLDLPPALQY
                     HDYLLGPVDKV"
BASE COUNT          360 a          501 c          542 g          286 t
ORIGIN      
        1 ggtccagcag cttcgtgccg gcctcttcgc gcttcgcaac cacccacccg cgagacgtga
       61 tggtatcgcc cagacagttc ggcccgttca tcgagaagcg cagcttgcgc aagaacgcct
      121 ccgggccggc ccagtccgtc gccaatcgcc tggagaaggt gatcatcgac gatgatgggg
      181 tgaacatacg ccctgaactc gaaggactgc catacgttcg aagtaagttg acaggggcgt
      241 ccgaggcggt gacgtcctcc ttgatcgaac cgcaccggct caactccccg ttcataatcg
      301 ggaacaagga gttcgagaag cggttgaagg aggagatgga atacaaaaaa ggagcgaacc
      361 tcgactggag gagggtggcc agggtgctga tgaggtacga ccccaactct ctggtgcatg
      421 gggtgttctt ctccaacctc gaggatggaa gggtacggct cgcccggctg ctcaccggct
      481 tcatcgaggc cgaggacatc aaggaggcgt actccggcgg ggtaaagaac aacagcatcg
      541 acccgagcgg caagatcagg ctcgccgatt cccagatcaa ggccggagtg tactcgaaca
      601 tcccctatca ccgggtggag tacgtggccc ggtccataag ggcgtacttc aacctcgatc
      661 tgagcatgct ccgctcctat gccctccccc cagaggggta tgacctcatc acagcgctct
      721 ccctttacaa ggtcgccaag ttcctcgagg acgatctgcg cctaaggacc atgtgcgacc
      781 tgaaggtgct ggggaagcgc atggtctcgg cacaggaggt cgcgttccca tcaagcgacg
      841 aggtgctggc ccaactgcgc ccggtcatcg atgcctgccg tgataaagga cttttcgcca
      901 agccgccggt gaccgacctg acggtggcca cgaagatgga gaaggaagag aaggaagggc
      961 cggtgcctga ggaagagcag tagaagcccc aggaggacgc atatgatctg catcaagctc
     1021 cgactactga caggaaggta ccatgccacg ccatggggga ggaacgtcaa cgaaggggtg
     1081 gctgaatggc ccccctcccc gttcaggctg ctccgggcga tctatgccac ctggaaaaga
     1141 cagttctccg agctggacga ggcgacggtc gataggattc tgaccgccct cgcaagcgaa
     1201 aggccggagt accggctgcc tccagctaag agctcgcacc ttcgcagcta cctccactcg
     1261 aacctgtcca atccagagaa gcgcttctca ggaaggcaga aggtgttcga tcagttcgtt
     1321 gtggtcatgc ccggccatga gatcaccatg acctggccta acgcgtcgct ggatgagggc
     1381 gacctcaagg ccctgcggga gctgctcggg gcgatgaact acctcggccg ctcagagtcc
     1441 tggtgcgaaa taggtctggg ggagcccatc gcgccatcga actgctggcc ggacgccggg
     1501 ggaccgttgg agcagggggg gtcacggatc cgggtggcgg gggtggccgc ccctgcggac
     1561 agcgaggggt ccagggaccg atggtgggac acattggcgt tggacagcaa cgacctggcg
     1621 aggctggggc tcgacctgcc tcccgccctg caatatcatg actatctcct cggtcccgtg
     1681 gataaggtc
//
LOCUS       DAOSYN010000272         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_53499_length_1678_cov_3.146026, whole genome shotgun sequence.
ACCESSION   DAOSYN010000272 DAOSYN010000000
VERSION     DAOSYN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53499_length_1678_cov_3.146026"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..536
                     /locus_tag="PLJ11_07945"
     CDS             150..536
                     /locus_tag="PLJ11_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOO04634.1"
                     /translation="MNSYPTRWTKQMKVIVDIVYDGQSPITADQVYERARATLPRISL
                     GTVYRNLNRLVQAGLISESKVGTVQCFFPHPYSNTHLECRVCGHLFSVPYDLSVHQVS
                     QETGLAIDGYELRLKGVCKGCASKCT"
     gene            527..1189
                     /locus_tag="PLJ11_07950"
     CDS             527..1189
                     /locus_tag="PLJ11_07950"
                     /inference="COORDINATES: protein motif:HMM:NF006184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOO04635.1"
                     /translation="MHIMEGFLSLEWALFWTVVSAPFVVYGFYHVRRIIREDPAQRTM
                     LALSGAFIFVMSSFKLPSVTGSSSHPTGTGFSTMFFGVGVTSVMSLIVLIFQALLLAH
                     GGLTTLGANVFSMGIAGPFVAYAAFRAMTKVRVPTPAKVFITVFLADVFTYITTAVQL
                     ALAYGPFLSSLTSFLAVFAITQIPLGIVEGILFTIFVDYLERARPDLTERILPRRVAD
                     EA"
     gene            1179..1463
                     /locus_tag="PLJ11_07955"
     CDS             1179..1463
                     /locus_tag="PLJ11_07955"
                     /inference="COORDINATES: protein motif:HMM:NF002780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HOO04636.1"
                     /translation="MRRSHYYLLGLLAVAVLLVSAVILNPSSDFTGTDDSGSDVINDV
                     DPDYEPWFESIWEPSGELQTLLFTLQAAIGALIIGYFIGTNRAHKERGDG"
     gene            1465..>1678
                     /locus_tag="PLJ11_07960"
     CDS             1465..>1678
                     /locus_tag="PLJ11_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04637.1"
                     /translation="MFELDEMAYRSAALRWSPAGKFVLALTMLVASLLANTLVVPLMV
                     LLVGTILLGYSVRFRFPKVIGALVLEG"
BASE COUNT          325 a          508 c          467 g          378 t
ORIGIN      
        1 ctttctcctc ttatatatca tttaaccaca aggccggagg cgtccctcct tcagcttcga
       61 taaggaaaag aagcgcaaag ctacatgata ttttttccgt gaatgagcga agtgtttaat
      121 tagcagacaa aatagtaata attactatta tgaattcata tcctacacga tggaccaagc
      181 agatgaaggt catcgtggac atcgtatacg atgggcagtc ccccatcacc gcggaccagg
      241 tctatgaaag ggcaagggcc accttgcccc ggataagcct aggcaccgtc taccggaacc
      301 tgaaccggct ggtgcaggcg gggctcatct ccgagtccaa ggtcggcacg gtccagtgct
      361 tcttccccca tccctattcc aacacccatc tggaatgccg ggtctgcggt cacctgttca
      421 gcgtgcccta cgacctgtct gtccatcagg tctcccagga aacaggcctt gccatcgacg
      481 ggtacgagct gaggctgaaa ggggtttgca aggggtgtgc atccaaatgc acataatgga
      541 ggggttcctg agcctggaat gggcgctttt ctggacggtc gtctccgccc cgttcgtggt
      601 gtatggattc tatcacgttc gaaggatcat aagggaggac ccggcccaac ggaccatgct
      661 ggccctgtcc ggcgccttca tattcgtgat gtcctcgttc aagctccctt ccgttacggg
      721 ctcctcctcc catcccactg gaaccgggtt ctcgaccatg ttcttcgggg tgggcgtaac
      781 gtccgtcatg tcgctgatcg tccttatctt ccaagccctg ctgctggccc atgggggcct
      841 caccacactg ggggcaaacg tcttctccat gggcatagcc ggcccgttcg tggcctacgc
      901 ggccttcagg gccatgacca aggtccgcgt cccaaccccc gccaaggtct tcatcaccgt
      961 gttcctggcc gatgtcttta catacatcac cacggccgtc cagctcgcgc tggcctatgg
     1021 gccgttccta agctcgctga caagcttcct ggcagtcttc gccatcaccc agattccgtt
     1081 ggggatcgtg gaggggatcc tgttcactat cttcgtggac tacctggaga gggcccggcc
     1141 agacctgacc gagaggatat tgccgaggag ggtggccgat gaggcgtagc cactattacc
     1201 tgttggggct attggccgtg gcggtcctgc tcgtctcggc cgtcatattg aaccccagct
     1261 ccgatttcac cgggacggat gacagcggga gcgatgtgat aaacgatgtg gaccccgact
     1321 acgagccctg gttcgagagc atctgggagc cttccgggga gctccaaacg ctgctcttca
     1381 cgcttcaggc cgcaatcggc gccctgatca tcgggtactt cataggcacc aacagagctc
     1441 acaaagagag aggggatgga tgatatgttc gaactggacg agatggccta ccgttccgcg
     1501 gcgctgcgat ggtcgcccgc aggaaagttc gtcctcgccc tgacaatgct ggtggcgtcc
     1561 ctcctggcga acaccctcgt cgtccccctg atggtgctgc tggtcggcac catcctcctg
     1621 ggctattccg ttaggttccg cttccccaag gtcatcgggg cactggtcct tgaaggga
//
LOCUS       DAOSYN010000273         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_53546_length_1677_cov_2.954377, whole genome shotgun sequence.
ACCESSION   DAOSYN010000273 DAOSYN010000000
VERSION     DAOSYN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53546_length_1677_cov_2.954377"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PLJ11_07965"
     CDS             <1..1293
                     /locus_tag="PLJ11_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO04638.1"
                     /translation="VEMRTEAGNTIRVTPETMVRAQREGRSRWVKASDIMAGDHVLQA
                     PSGPSKGRLSLGRVEGVAEVRAALPPFVYDLTVEGAHSFIANGFLVHNTAAAVKDEFG
                     DGRWTLEAGALVLADKGLAAIDELDKMTEQDRSAMHEAMESQRVSVAKAGITASLQCR
                     CSMLGAANPKLGRFDDAEALASQINMPPALLSRFDLIFALTDKPNAARDQKIAEHILK
                     GHVRGELMNVPNPEEIKGIDYQRIMEETGVLRPVYDREFMRKYVAFAKRYTPVLGDEA
                     RQMIIDKYLSIRKLGEKAGSSVPITARQLEAFIRLSEASARIRLSQVVEAEDAERAVR
                     IVEHYLKKMASDEGTIDVDKLMTGTTRNERNRIALVRQLIHDNSEPGTGASEITLIQR
                     AADRNLTEAELKAAIAKLKQSGDIYEPQAGHYKLTTLN"
     gene            1342..1641
                     /locus_tag="PLJ11_07970"
     CDS             1342..1641
                     /locus_tag="PLJ11_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 family protein"
                     /protein_id="HOO04639.1"
                     /translation="MCEVLVKVYRRGGDMVIAACDRGLLGRRLREGELRLEVSTEFYG
                     GDQGGEAMLLSRLAMATVANLVGEDVCRIAAEHGYIEMASVIRIEGVPHAQMVRW"
BASE COUNT          321 a          545 c          579 g          232 t
ORIGIN      
        1 gtggaaatgc gcaccgaggc tggcaacacc atccgcgtca ccccggagac catggtccgc
       61 gcccaaaggg aagggcgttc ccggtgggtc aaagcgtctg acattatggc gggcgaccac
      121 gtcctccagg ctccatctgg cccatccaag ggaaggctct cgctcgggag ggtcgagggc
      181 gtcgcggagg tgcgcgccgc cctgccgccg ttcgtctacg acctgaccgt ggaaggggcc
      241 cacagcttca tcgccaacgg gtttctcgtg cacaacaccg cggccgcggt caaggacgag
      301 ttcggggacg ggcggtggac gctggaggcg ggggcgctgg tcctcgcgga caaggggctg
      361 gcggcgatcg acgaactgga caagatgacg gagcaggacc gctccgccat gcatgaggct
      421 atggagtcac agcgggtgag cgtcgccaag gccggcatca ccgcctccct gcagtgccgc
      481 tgctccatgc tgggggcggc gaaccccaag ctggggcgct tcgacgacgc ggaggccctg
      541 gcctcccaga tcaacatgcc accagcgctc ctctcgcgct tcgacctcat cttcgccctc
      601 acggacaaac cgaacgcggc cagggaccag aagatcgccg agcacatcct caaggggcac
      661 gtgcgcggcg aactgatgaa cgtgccgaac cccgaggaga tcaagggcat cgactaccag
      721 cgcataatgg aggagacggg cgtcctccgc cccgtctacg accgggagtt catgcgcaag
      781 tacgtggcct tcgccaagcg ctacacgccg gtgcttgggg acgaggcgag gcagatgatc
      841 atcgacaagt acctctcgat ccgcaagctg ggggagaagg ccggctcctc cgttccgata
      901 accgccaggc agctggaggc cttcatccgc ctctcggagg cgtccgcccg catccgcctc
      961 tcgcaggtgg tcgaggcgga ggacgcagag cgcgcggtca ggatcgtgga gcactacctg
     1021 aagaagatgg catcggacga ggggacgatc gacgtggaca agctcatgac cgggacgacg
     1081 cgcaacgagc ggaaccgcat cgcgctcgtc aggcagctca tccacgacaa ttccgagccc
     1141 gggacgggcg cctccgagat cacgctcatc cagagggcgg cggaccggaa cctcacggag
     1201 gccgagctga aggccgccat cgccaaactg aagcagagcg gggacatcta cgagccccag
     1261 gcgggccact acaagctcac caccctcaac tgaggcccta cagaggaaag gtttatctcc
     1321 ctgccctatg actcccatcc gatgtgcgag gtcctggtga aggtgtaccg gcggggcggg
     1381 gacatggtca tcgccgcgtg cgaccgcggc ctcctcggcc gcaggctcag ggaaggggag
     1441 ctacgcctgg aggtcagcac cgagttctac ggcggggacc aggggggcga ggcgatgctg
     1501 ctcagccgcc tggccatggc cacggtggcc aacctggtgg gggaggacgt ctgccgcatc
     1561 gccgccgagc acggctacat cgaaatggcg agcgtcatcc gcatcgaggg cgtcccgcac
     1621 gcccagatgg tgaggtggtg agatgccctt ctgtgtcagg tgcggggccg agggccc
//
LOCUS       DAOSYN010000274         1669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_53905_length_1669_cov_2.700743, whole genome shotgun sequence.
ACCESSION   DAOSYN010000274 DAOSYN010000000
VERSION     DAOSYN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53905_length_1669_cov_2.700743"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="PLJ11_07975"
     CDS             <1..1472
                     /locus_tag="PLJ11_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOO04640.1"
                     /translation="YFGLSDETLCRVVAASSGQLFSRLRKFADEGPDEEVRRAYTVLS
                     GLLRRCRTVPVSEVLSALLQETGVLGVYAGLPNGRQMIANVEQLLGTVRRKEQEGPFT
                     LYDLIDWLNASMANSEREGQAQLEAVGDAVRIMTVHASKGLEFPVVLVPEAEAAPREE
                     RDPAALTPAGLFAEVPTPDLSTTYPPAPLRPALRELKARMEAESLRLFYVAATRARDH
                     LVVLGGRKKEGAWEPIDKDRRDWFALTLNGLGIADAEAGTIPLDEDGRYALSLETFEA
                     AGGGLSLEPVRPCVVPEGLERTPAARVRPEPRVELVFPSALDERTEGRLKGGEGSELK
                     RLLTSRGMDAAEYGTLVHEALRGKEAGTLLRFSRASLSPEETEWAARELQGIVARFMA
                     SEPMASRAEGGRDLTELPFELREGDRLYRGTIDRLVRLRDGTWALIDYKTVGLREPAA
                     VAEAFRGQMDVYIKAAQGLVGERVRAFLYLTEAGTVVEL"
BASE COUNT          280 a          490 c          667 g          232 t
ORIGIN      
        1 cctacttcgg cctctcggac gagacgctgt gccgcgtggt cgccgcctcc tccggccagc
       61 tcttctcccg cctgcggaaa ttcgccgatg aggggccgga cgaggaggtc agaagggcat
      121 acacggtcct gagcgggctg ctgagacggt gccggaccgt gcccgtgagc gaggtgctga
      181 gcgccctgct gcaggagacg ggcgtgctgg gcgtgtacgc cggcctgccg aacggcaggc
      241 agatgatagc gaacgtggag cagctcctgg gaacggtccg gaggaaggag caggaggggc
      301 cgttcaccct gtacgacctg atagactggc tgaacgcgag catggcgaac tcggaaaggg
      361 aggggcaggc ccagctggag gcggtggggg acgcggtgcg catcatgacc gtgcacgcct
      421 cgaaggggct ggagttcccg gtggtcctag ttccggaggc ggaggcggcg ccccgggagg
      481 agagggaccc ggccgccctg acgcccgcgg ggctgttcgc ggaggtgccg accccggacc
      541 tctccacgac gtatcctccg gcgcccctgc gccccgccct gcgggagctg aaggcaagga
      601 tggaggcgga gagcctgcgg ctgttctacg tcgccgcgac gagggcgagg gaccatctgg
      661 tggtcctcgg aggaaggaag aaggaaggcg cttgggagcc gatcgacaag gacaggcggg
      721 actggttcgc gctcacgctg aacgggctgg gcatagcgga cgccgaggcg gggacgatac
      781 cattggacga ggacggccgg tatgccctgt cgctcgagac gttcgaggcc gccggaggag
      841 gcctttccct cgagccagtc cgcccgtgcg tggtgccgga ggggctggaa cggacgcccg
      901 cggcccgcgt ccgcccggag ccgagggtgg aattggtgtt cccctcggcc ctcgacgaga
      961 ggacggaggg gaggctgaag gggggcgagg gctcggagtt gaagcggctg cttacctcca
     1021 gggggatgga cgccgccgag tacggcaccc tggtgcacga ggcgctgcgc gggaaggagg
     1081 cggggacgtt gctgcgcttc agccgcgcct ccctctcccc cgaggagacg gaatgggcgg
     1141 cgagggagct gcaggggatc gtagcgaggt tcatggcctc cgagccgatg gcgtcccggg
     1201 ccgagggcgg cagggacctg accgagctgc ccttcgagct gagggagggg gacaggctct
     1261 acagggggac gatcgaccgg ctggtgcgat tgagggacgg cacgtgggcg ctgatcgact
     1321 acaagaccgt gggcctcagg gagccggcgg cggtcgcgga ggcgttcagg gggcagatgg
     1381 atgtctatat caaggcggcc caagggctcg tcggcgaacg ggtgcgtgcg ttcctctatc
     1441 tgacggaggc cgggacggtg gtcgagctgt gaagggcggg aggcgaggac cggcctccca
     1501 tcacgcttcc ataggaccgt cttccagctg tcaggaaggc cgccagggac ggacgaacaa
     1561 cctggtccaa gggggcctgg cgctccccgc acgttccacg taaacaaaag ggttcggcgc
     1621 taatggggga tgtccgaaga ggcccaagga agtcccccgt ttccttcag
//
LOCUS       DAOSYN010000275         1657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_54445_length_1657_cov_2.683521, whole genome shotgun sequence.
ACCESSION   DAOSYN010000275 DAOSYN010000000
VERSION     DAOSYN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54445_length_1657_cov_2.683521"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLJ11_07980"
     CDS             <1..329
                     /locus_tag="PLJ11_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04641.1"
                     /translation="SKYDLALGRLPKNVVEAGTNDNDLDFIQLFASSEEELRREFPKL
                     MRSLKREGVLWISYPRTSSEIDSDLTGVTVRDVGNSYGMTEVKISSLDEDWAGIKFVS
                     KRKDTD"
     gene            379..546
                     /locus_tag="PLJ11_07985"
     CDS             379..546
                     /locus_tag="PLJ11_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04642.1"
                     /translation="MKLSGREMTVFIALIAPLLFLIAALLVPVNIWVIMLAILWLGTG
                     LTILYLPSAEE"
     gene            complement(547..756)
                     /locus_tag="PLJ11_07990"
     CDS             complement(547..756)
                     /locus_tag="PLJ11_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOO04643.1"
                     /translation="MRDEHGPPGKRPVAEGGVYDVTITDIGERGDGIGKIEGLVIIVP
                     DATPGETVRVRITRVERKVAFGRRL"
     gene            820..>1657
                     /gene="fen"
                     /locus_tag="PLJ11_07995"
     CDS             820..>1657
                     /gene="fen"
                     /locus_tag="PLJ11_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HOO04644.1"
                     /translation="MGVDLSDLVVAKDLEPRDLMGRTVAVDAYNAIYQFLSVIRQPDG
                     TPLMDPRGRITSHLEGLLNRNANLVEMGLRPAYVFDGIPPERKEATVRERRERRESAL
                     RDWERAKAEGDIKRAYSKASQSSRITNEIVESARILLIHLGLPVVQAPEEGEAQSAYM
                     CAKGDVWASASQDFDSLLFGAPRLVRNLTLAGRRKMPGREEYREVRMQLIDRQETLAT
                     LGLSGPQLVDLCILMGTDFNPGIAGVGPKKALRLIKQHGTLVGALQALGVDDPDMLEV
                     RGI"
BASE COUNT          308 a          523 c          530 g          296 t
ORIGIN      
        1 cttccaagta cgacctggcc ttgggacgcc tgcccaagaa cgtggtggaa gcggggacca
       61 acgacaacga cctggacttc atccagcttt tcgcctcatc ggaggaggag ctgcgtcgcg
      121 agttccccaa gctgatgcgg tccttgaagc gggagggggt cctttggatc tcctacccaa
      181 ggacctcctc cgagatcgat tccgacctga cgggggtcac cgtgcgggat gttgggaaca
      241 gctacggcat gaccgaggtg aagatctcct ccctggacga ggattgggcc gggatcaagt
      301 tcgtgagcaa gcggaaggac acggactgag ctcagtaaag tacatatcaa gaggccggca
      361 ttgcggggtg agatgcacat gaagctatct ggcagggaga tgacggtatt catcgcgctc
      421 atcgccccgt tgctcttcct catcgccgcc ctccttgtcc cggtcaacat ctgggtcata
      481 atgcttgcca tcctctggtt gggtacgggg cttacgatcc tctacctgcc gagcgcagag
      541 gaatgatcag agcctcctgc cgaaggccac cttcctctcc acacgcgtga tccgcaccct
      601 cacggtctcc cccggagtgg cgtccggaac gattatgacc agcccttcga tcttgcctat
      661 gccgtccccc ctctccccta tatcggttat ggtgacgtcg tagaccccgc cctccgccac
      721 cggccttttc ccgggcggac catgctcatc gcgcatgcgc gaaccattgg tcccgtggac
      781 aataacttta tggctgagag ggatacgccc tcgatcgata tgggagtgga cctatccgac
      841 ctggtcgtgg ccaaggacct ggagcccagg gatctgatgg gacggacggt cgccgtcgac
      901 gcctacaacg ccatctacca gttcctgtcg gtcataaggc agccggacgg caccccgctc
      961 atggaccccc gcggccgcat aacctcccat ctggaggggc ttttgaaccg aaacgccaac
     1021 ctggtggaga tggggctgcg cccagcctac gtcttcgacg gcatcccccc ggagaggaag
     1081 gaggccacgg tccgggagcg gcgggagcgg cgggaatcgg ccctgaggga ttgggagcgg
     1141 gccaaggccg aaggggacat caagcgggcg tactcgaagg cgagccagtc ctcccggatc
     1201 accaacgaga tcgtggaatc ggcgcgcatt ctgctcatcc atctcggcct tccggtggtc
     1261 caggcccccg aggaggggga ggcccagtcc gcctacatgt gcgccaaggg cgacgtgtgg
     1321 gcctcggcct cccaggactt cgattcgctg ctcttcggag ccccccgcct cgtccgcaac
     1381 cttacgctcg cgggccggcg caagatgccg gggagggagg agtataggga ggtcaggatg
     1441 cagctcatcg accgccagga gacgctggca acgttggggc tcagcggccc acagctggtg
     1501 gacctctgca tcctcatggg cacggacttc aaccccggca tagcgggggt ggggccgaag
     1561 aaggcgctga ggctgatcaa gcagcatggg acgctcgttg gagcgcttca ggccctgggc
     1621 gtcgacgacc cggacatgct cgaggtgcgg ggcatct
//
LOCUS       DAOSYN010000276         1655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_54519_length_1655_cov_3.212500, whole genome shotgun sequence.
ACCESSION   DAOSYN010000276 DAOSYN010000000
VERSION     DAOSYN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54519_length_1655_cov_3.212500"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLJ11_08000"
     CDS             complement(<1..249)
                     /locus_tag="PLJ11_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04645.1"
                     /translation="MKGRAEARGAVTIVNAIAAGKGCAVGVDLRTWAEVELVPGEGAE
                     VRLEGLPDEPTTLAELCALEVLERFSVRRRAIVRTGSQL"
     gene            complement(246..>1655)
                     /locus_tag="PLJ11_08005"
     CDS             complement(246..>1655)
                     /locus_tag="PLJ11_08005"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOO04646.1"
                     /translation="LAVAREALARGAECLELRFDAMAELPGDLTPFSQLEGRKIATLR
                     EREQGGGWEGTTEAKWEFLRRAAKAGFDIDLELGHPLVSRAHLLRPAKVIVSYHDLKK
                     TPSVARIIEVLVECSARSDMAKAAFQVRTVHDLAQLVEAAQLFALTRERFTLIGMGEL
                     GMATRVLAHRMGSLLSYAAVSPGKETAPGQLDLETAKWLGKEPMVTGIVGSPLDHTVS
                     PALHTAAYRASAVRGTYLKWVTMVDELEDLLDVVDALGIRGFNVTIPHKEGILPLLDS
                     VDAVAEGIGAVNTVINEGGRLVGRNTDVIGVQRTFEHHKVEVKGRPALVLGAGGASRA
                     VLQFLTTEGAEVDITNRTMGKAEALARMFGARPVALRAAEERRYDIIVNCTPLGMKGF
                     PQEAPISPHALHEGQFVMDTVYNPPVTPLLAEASRKGARAVNGMDMLIYQALASFELW
                     TGKAVEREVMERAFREALP"
BASE COUNT          233 a          646 c          515 g          261 t
ORIGIN      
        1 gagctgggag ccggtgcgga cgatcgccct ccgcctaacg gagaagcgct ccagcacctc
       61 cagggcgcag agctcggcca acgtcgtcgg ctcgtccgga aggccctcca gccggacctc
      121 ggccccctcc cccgggacca gctccacctc cgcccaggtg cgcaggtcca cgccgacggc
      181 gcaccccttc ccggcggcga tggcgttgac gatggtgacg gcccccctgg cctccgccct
      241 ccccttcacg gcagcgcctc cctgaaggcc ctctccatga cctcccgctc cacggccttc
      301 ccggtccaca gctcgaagga ggccagggcc tggtagatca gcatgtccat gccgttgacc
      361 gccctcgccc ctttgcggga ggcctcagcc agcagcggcg tcaccggcgg gttgtacacg
      421 gtgtccatga cgaactgccc ctcatggagc gcgtgcgggg agatcggcgc ctcctgcggg
      481 aagcccttca tgcccagggg ggtgcagttc actatgatgt cgtaccgccg ctcctccgcc
      541 gcccgcagcg ccaccgggcg ggcgccgaac atcctggcca gggcctccgc cttccccatg
      601 gtccggttgg tgatgtccac ctcagccccc tcggtcgtca ggaactggag gacggcacgg
      661 gaggcgcccc ccgctcccag caccagcgcc ggcctcccct tcacctccac cttgtggtgc
      721 tcgaacgtcc gctgcacgcc gatgacgtcc gtgttcctgc cgaccagcct ccccccctca
      781 ttgatcacgg tgttcaccgc cccgatcccc tcggccacgg cgtccacgct gtccaggagc
      841 ggcaggatgc cctccttgtg cgggatggtc acgttgaagc cccggatccc cagggcgtcc
      901 accacgtcca ggaggtcctc cagctcgtcg accatcgtga cccatttgag gtacgtcccg
      961 cggaccgccg aggccctgta ggcggcggtg tgcagggccg gggatacggt atggtccagc
     1021 ggggacccca ctatgccggt caccatcggc tccttcccca gccacttggc cgtttccagg
     1081 tccagctggc cgggggcggt ctccttgccg ggcgagacgg ccgcgtagga gagcagggag
     1141 cccatcctgt gggccaggac cctcgtcgcc atgcccagct cccccatgcc tatgagggtg
     1201 aagcgctccc gggtgagggc gaacagctgc gcggcctcca ccagctgcgc caggtcatgc
     1261 accgtccgga cctggaaggc ggccttggcc atgtccgagc gggcggagca ctccacgagg
     1321 acctcgatga tcctggcgac ggagggcgtt ttcttcaggt catggtagga gacgatgacc
     1381 ttggccggcc ggaggagatg cgccctgctc accagcggat gcccgagctc caggtctatg
     1441 tcgaacccgg ccttggcggc ccgccgcagg aactcccatt tcgcctcggt cgtcccctcc
     1501 cagccccctc cctgctccct ctcccgcagg gtcgcgatct tccggccctc aagctgagag
     1561 aacggcgtga ggtcgccggg cagctcagcc atggcgtcga agcggagctc gaggcactcc
     1621 gcccccctcg ccagcgcctc cctggccacc gccaa
//
LOCUS       DAOSYN010000277         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_54567_length_1654_cov_3.632270, whole genome shotgun sequence.
ACCESSION   DAOSYN010000277 DAOSYN010000000
VERSION     DAOSYN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54567_length_1654_cov_3.632270"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..622)
                     /locus_tag="PLJ11_08010"
     CDS             complement(188..622)
                     /locus_tag="PLJ11_08010"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOO04647.1"
                     /translation="MEMPTGYDLIGHSHDFRMHLLKRLAACLVDVAVVFVPVTVAIYL
                     LDLEPKDLLAGVLSGFVWFLYSALAEARTGTTFGKRIVGLVVVSKDGPMTLSKGIVRN
                     VPKMFWFIFLPVDFLVGLSREGDPRQRWVDGVAGTTVIKENQ"
     gene            932..1258
                     /locus_tag="PLJ11_08015"
     CDS             932..1258
                     /locus_tag="PLJ11_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04648.1"
                     /translation="MLNGKVEPKAIAGGLLAAIGIAGLLLTNLWALWLVPLAAAAVLL
                     AVAVTDRYEYECQKCHAKYRLGARGLLASRHGRDANGSWAISRCPHCGAITKAREARS
                     HDQEKG"
     gene            1239..>1654
                     /locus_tag="PLJ11_08020"
     CDS             1239..>1654
                     /locus_tag="PLJ11_08020"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOO04649.1"
                     /translation="MTRRRDRGSGSKRTILFICKGNASRSQMAEGYVNARMGDRYQAS
                     SGGLSPERVSRKAVAVMAEIGIDISGQRSKSVEEFYGKEFDCVVTLCDEAEEECPHFL
                     RGKDYIHKNFEDPCKARGTEEELLAIYRRTRDDIIR"
BASE COUNT          378 a          499 c          482 g          295 t
ORIGIN      
        1 gcgaatggat tggaccatac ataaatattt tataataaat attgataaca atataaatca
       61 attaaaatct aattgcctga agttggcaaa gtgtcaggtt aaattttgca acaaggcctc
      121 ctcaaaaaat tcgataggca ttccgctgtc gatcatactt catgaccaca cgttattgaa
      181 ccacgcgtca ttgattttct ttgataacgg tcgttcctgc cacgccatcc acccaccgct
      241 gcctcgggtc cccctcgcgg ctcaggccga ccaggaagtc gacaggcagg aagatgaacc
      301 agaacatctt cgggacgttc ctgacgatcc ccttcgacag cgtcatcggc ccgtccttgg
      361 agacgaccac gagtcctacg atccgtttac cgaacgtggt cccggtccgc gcctccgcca
      421 gcgcggaata gaggaaccaa acgaacccgg agagcacccc ggccagcaga tccttcggct
      481 ccaggtccaa gaggtagatc gccacggtca ccgggacgaa caccactgcc acgtccacca
      541 ggcaggccgc tagccgcttg agcaggtgca tgcggaaatc atggctgtgc cctatcagat
      601 cgtacccggt cggcatctcc atcccgaggg tatatatggc tcacgctatt ttatttttta
      661 tgcaaaaaaa ttaatctagc cactcagctc gcccgtctct catcacctgt cagagctggg
      721 tctatcatca tcgtggcggg cgtccatcgc ccccctcgga gataaagcta ttccgacaag
      781 gccctcgaca aggcctacgt cccatgcgaa ccgccggcac cctcccttat atttgggaac
      841 gcgatatccg gccgagcgaa gaggaacgaa gcgtcccgtc ggcgctcgaa ggctccgggc
      901 cgtcatgggc gccgccgtca ggtgtcattt catgctcaac ggaaaggtcg aacccaaggc
      961 gatcgcgggc gggctgctgg ccgccatcgg catagcgggg ctcctgctta cgaacctgtg
     1021 ggccctgtgg ctggtgccgc tcgcggccgc cgccgtccta ctggccgtgg ccgtcacgga
     1081 tcgctacgag tacgaatgcc agaagtgcca tgccaagtac aggctcggag ccaggggcct
     1141 cctggcctcc aggcatggac gggacgcgaa cgggagctgg gccatctccc gctgccccca
     1201 ctgcggggcg ataaccaagg cccgggaggc acgatcacat gaccaggaga agggataggg
     1261 gatcggggag caaacggacg atactcttca tatgcaaagg gaacgcctcc cgctcgcaga
     1321 tggccgaggg gtacgtcaac gccaggatgg gcgacaggta ccaggcgagc agcggcggcc
     1381 tgagcccgga gagggtcagc cgcaaggcgg tggccgtaat ggccgagatc gggatagaca
     1441 tctccgggca gcggtccaag agcgtggagg agttctacgg caaggagttc gactgcgtcg
     1501 tcacgctctg cgacgaggcc gaggaggaat gcccccactt cctacgtggg aaggactaca
     1561 tccataagaa cttcgaggac ccatgcaagg cccgagggac ggaggaggag ctcctcgcga
     1621 tatacaggag gacgcgggac gacatcatca ggtg
//
LOCUS       DAOSYN010000278         1647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_54868_length_1647_cov_3.152010, whole genome shotgun sequence.
ACCESSION   DAOSYN010000278 DAOSYN010000000
VERSION     DAOSYN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54868_length_1647_cov_3.152010"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1157)
                     /locus_tag="PLJ11_08025"
     CDS             complement(117..1157)
                     /locus_tag="PLJ11_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOO04650.1"
                     /translation="MLEPLDDLPGMKATDPSDLLSQLTSFPRQIEWSKEVTFPELPGV
                     DNVVICGVGGSAIAGDVIIDLLSPSIPVPVIVSRNVVLPGFAGKGTLVVVVSYSGNTA
                     ESLEQYSDALRRGCRVAVVTSGGKLEEFAIADSIPLIKVPAGNQPRASLGYLLSALAL
                     VFQRAGLGQLHDELLRAVPDLRSYIASIAPDVPYGMNQAKRLAKAMAGGVVVVYAPRQ
                     VRSVALRWQNQLNENAKAVAFSGEVPEMDHNQLVGWLEGGKGCPCRPLLLMPSDMGPT
                     VRRMSEVTLQMLNERGLDPVLVTLPGESLLENVLHGVALGDMASYYLAIIRGVDPAPV
                     ASITEFKGRIAP"
     gene            1280..>1647
                     /locus_tag="PLJ11_08030"
     CDS             1280..>1647
                     /locus_tag="PLJ11_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOO04651.1"
                     /translation="MSDEMIERALEDIRKGRMVMIFDADDRERETDMVIASQFITPAS
                     IREMRKNAGGLICTTAHNDICRKLDLPFLADVLACNHDRHPVLKGLAPNDIPYDTKSA
                     FSITINHRRTFTGITDKDRAL"
BASE COUNT          313 a          513 c          537 g          284 t
ORIGIN      
        1 ccgcacccgc caggggcagc ttaggaggct tggctggacg ctgcaccgtg tctggagcgt
       61 cgaatggttc cgggatccgg agggggaaat ggagaggata gaggaggcgc tgcgcgctag
      121 ggggcgatgc gccccttgaa ctccgtgatg gaggctacgg gggcggggtc cacccccctt
      181 atgattgcca ggtagtagct cgccatgtcc ccgagcgcga ccccatggag gacgttctcc
      241 agcaggctct ccccaggcaa cgtcacgagc accgggtcca ggcctcgctc gttgagcatc
      301 tgcagcgtca cctccgacat gcggcggacg gtcggcccca tgtcggaggg catgagcagc
      361 agggggcggc acgggcagcc cttcccaccc tccagccatc cgaccaactg gttgtggtcc
      421 atctccggca cctcgccgct gaaggccacc gccttggcgt tctcgttgag ctggttctgc
      481 catcgcaggg caacggaccg gacctggcgc ggggcgtaca caaccaccac gcccccggcc
      541 atggccttgg ccagccgctt ggcctggttc atgccatagg gcacgtccgg agcgatggat
      601 gcgatatagg agcggaggtc tgggacggcc ctcaggagct cgtcgtgcag ttgcccgagg
      661 ccggccctct ggaagacgag cgcaagggcg ctgagcaggt acccgagcga ggcccgcggc
      721 tggttgcctg cgggcacctt gatgaggggg atggaatcgg cgatcgcgaa ctcctcgagc
      781 tttccgccgg aggtgaccac cgcgacacgg catccccgcc gcagggcgtc gctatactgc
      841 tccaggctct ccgcggtgtt gcctgagtag ctcaccacca cgaccagggt tcccttccct
      901 gcgaagcccg gaaggacaac gttccgggac acgatgaccg gaacggggat ggatggggaa
      961 agcaggtcga tgatgacgtc ccccgctatg gccgagcccc cgacgccgca tatcacgaca
     1021 ttgtccacgc ctgggagttc ggggaacgtt acctcctttg accattcgat ctggcgcggg
     1081 aaggaggtga gctgagagag caggtctgag gggtccgtag ccttcattcc gggcagatca
     1141 tccagcggtt cgagcatcag ggagcatacg cgatggcctc tattttattt tgccctgcgg
     1201 gccctggccc gttctcatta ccatttgaat tgtagtaacg caaggaatat actagtacct
     1261 ccctgttgag ggagccatca tgagcgacga gatgatcgag agagcgttgg aggacatacg
     1321 caagggaagg atggtcatga tcttcgatgc ggacgaccgg gagagggaga cggacatggt
     1381 catcgcctcc cagttcatca cccccgcgtc catacgggag atgcgcaaga acgccggggg
     1441 gctgatatgc acgaccgccc ataatgacat ctgccgcaag ctggacctgc cgttccttgc
     1501 ggatgtgctt gcatgcaatc acgacaggca ccctgtgctg aaggggctgg cgccgaacga
     1561 catcccgtac gacacgaagt ccgccttctc catcaccatc aaccaccgcc ggaccttcac
     1621 cgggatcacg gacaaggaca gggctct
//
LOCUS       DAOSYN010000279         1641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_55167_length_1641_cov_2.044767, whole genome shotgun sequence.
ACCESSION   DAOSYN010000279 DAOSYN010000000
VERSION     DAOSYN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55167_length_1641_cov_2.044767"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLJ11_08035"
     CDS             <1..517
                     /locus_tag="PLJ11_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO04652.1"
                     /translation="DQYGLSKYIMSKYTERAENIVDLRVFGCFGPHEDWEIRFISNAM
                     CKALYGMPITLRQNVFFDYLWVGDLVQMTRWAMRHHTRYNHYNACSGSHIDLLTIAEM
                     VKEVTGSEVPIIVAEPGLKAEYSGTNARLRREMVDLQITPMREALERLRDYYQENLAL
                     IDRSLLMRDKH"
     gene            527..1171
                     /locus_tag="PLJ11_08040"
     CDS             527..1171
                     /locus_tag="PLJ11_08040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO04653.1"
                     /translation="MRIEAILLDLDGTIVDSFRDIHRALGDAAEDLGAQRPSEGSVRA
                     LLHLRLDQLVPRLMPQLNAQDVIDRFRAHYDGGSYVETAPYPQVERTMRELARRNVKL
                     FVVTNKRRMAAEAILDRLGLCQYIKGLATADDTDPPSDKASLVARLLASHSLQVERTI
                     LVGDTISDREAAEANGLRFVLAAYGYGGPESRGPNFAPVRISHFGQLLDVCNSF"
BASE COUNT          366 a          443 c          508 g          324 t
ORIGIN      
        1 tgaccagtat gggctatcaa agtacatcat gtcaaagtac acggagaggg cggaaaatat
       61 cgtggacctc cgggtgttcg gatgcttcgg cccccatgag gattgggaga tacgcttcat
      121 ctccaacgcc atgtgcaagg ccttgtacgg gatgcccatc accctccggc agaacgtgtt
      181 cttcgactat ctttgggtgg gggacctggt gcaaatgacc cgatgggcca tgcgccatca
      241 tacccggtac aaccattaca atgcctgcag cgggtctcac atcgacctat tgaccatcgc
      301 cgagatggtc aaggaggtca cagggagcga agtgccgatc atagtggcgg agcccgggct
      361 taaagcagag tacagcggga ccaacgctcg gttgagaaga gaaatggtgg atctgcagat
      421 cacacccatg cgggaggcgc tggagaggct cagggactat taccaagaaa acctggccct
      481 catcgatcga tcgttgctga tgagggataa acattgaggc ggtgcaatga ggattgaggc
      541 catcctgctc gatctggatg gcacgatcgt ggattcattc cgtgatatcc atcgtgcctt
      601 gggggatgcg gccgaagacc tgggcgccca gaggcccagc gagggctcgg tgcgagccct
      661 cctgcatctc cggttggacc agctcgtccc ccggctcatg ccccaactga acgcgcagga
      721 cgtcatcgac cgcttccgcg cccattatga tgggggcagc tatgtggaga ccgcccccta
      781 cccgcaggtc gagcggacaa tgcgcgaact ggccagacgc aatgtaaagc tgttcgtggt
      841 caccaataag cgaaggatgg ccgcggaggc gatccttgac cgtctcggtc tatgccaata
      901 tatcaagggg ttggccaccg ccgacgacac ggacccgcca agcgacaaag cgtcattagt
      961 ggcccgattg ctggccagcc acagtttgca ggtggagcgc accatcctcg tgggggatac
     1021 gatcagcgat cgggaggcag cggaggccaa tggcctgcgg ttcgtgctgg ccgcctacgg
     1081 ttacggaggg cccgagtcac gtggaccaaa cttcgccccg gtccggatat cccatttcgg
     1141 gcagcttctg gacgtctgca attccttttg atggggatgg agagggcatc cagtccctcg
     1201 ccgctccccg gaggcgaatg ccatttgctt tacttcgttt cagtgctgga ggttctgggg
     1261 ccatgggcgg tgcaaggccg aaggcgtgag atgtacaaag tgacctcgtc gcgtgctatt
     1321 ggggcgaggt tcccagtccc agtatggatc gccatgcgga ccatgcaatg catttcagca
     1381 catataatgg ctcaggccgc agtaggtcga tgcgcattcc ggggatagaa caggtcatca
     1441 ggacggtctc cctggggtgc aaggatgaca aggggcatgc atgaccttcc cgaagaaatc
     1501 atcggaattg catgtcagag aatggcgtcc aacacattgg gaaaacataa agggataggt
     1561 tcatgagtga tgttccacat ataagtccaa ggttcgaggg caccaagcca tatcgatttc
     1621 cctcgtggca gggggatcaa c
//
LOCUS       DAOSYN010000280         1628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_55737_length_1628_cov_2.744437, whole genome shotgun sequence.
ACCESSION   DAOSYN010000280 DAOSYN010000000
VERSION     DAOSYN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55737_length_1628_cov_2.744437"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..>1628)
                     /locus_tag="PLJ11_08045"
     CDS             complement(149..>1628)
                     /locus_tag="PLJ11_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylamine methyltransferase corrinoid protein
                     reductive activase"
                     /protein_id="HOO04654.1"
                     /translation="IIIETVDKLIGTMGAKPEEIIRVSVCGNPAQMSMFEKIEIRDLA
                     FAGQSILKRLNVKLPERKAHTIKAEELGMRSVKGTAEVRIPPSIRHEIGADALAMVMK
                     TGLMEKNETCMVTDYGTNAEMGLFHKGELYTGSAAAGPAMEGQSIDFGMLAAPQAISD
                     LALADDGKWYNIVLNDNLHPVKSSLVDPTTGEERRLDGVVARGITGTGTVAAMALGLE
                     TGVIKLPYVATPDHRIHLTNGIYFGEEDVREAGKAIGAIRAGHRTLIEEVGVEDEAIK
                     TMYMAGASGTYVDPIKAQTVGLIPRVLDKTVQAGNTSLMMSYDILVDDDGLDKMQDVA
                     SAISSKHIMFATSKVFEEIFVNEIAYWTEGMSLELYNDMVKHAGLRPLPEIKRPKETV
                     RLVLSDIPVIGARGLRTLDDVGVYLIGSFEGCIGCKKCQKECPERALQVSPLGDRKDK
                     RFQIKVNTEHCLGTACKKCQSVCPESVYDFSALKIVEKGVDA"
BASE COUNT          264 a          541 c          477 g          346 t
ORIGIN      
        1 cggcgtccat cagtaccttc ttccccctgt cggtctccac caggagggag aggccgtgct
       61 cggagaggaa ccccccgccc gccgttgaga tggagcggtc ggggtcctcc ctaccgctgt
      121 tatcgtcgac cacgcaggta aggcgcacct acgcgtccac tcccttctcg acgatcttca
      181 gtgcgctgaa gtcgtagacg ctctccgggc atacggactg gcatttcttg cacgccgttc
      241 caaggcaatg ttcggtgttc acctttatct ggaacctctt gtccttcctg tccccgaggg
      301 gagagacctg cagcgccctc tctggacatt ccttctggca tttcttgcag ccgatgcagc
      361 cctcgaagga gccgataagg tacaccccga catcgtccag ggtccgcaat ccgcgggccc
      421 cgatcaccgg aatgtccgag aggacgaggc gcacggtctc cttgggacgt ttgatctcgg
      481 gcagggggcg cagccccgca tgctttacca tgtcattgta caactccagg gacatgccct
      541 ccgtccagta ggctatctcg ttgacgaaga tctcctcgaa caccttgctc gtggcgaaca
      601 tgatgtgctt gctggagatc gcgctcgcca catcctgcat cttgtccagg ccgtcatcgt
      661 ccaccagtat gtcgtaggac atcatcaggg aggtgttgcc agcctgtacg gtcttgtcca
      721 gcacccgggg gatgaggccg acggtctgcg ccttgatggg atcgacgtac gtcccggacg
      781 ccccggccat gtacatggtc ttgatcgcct cgtcctccac ccccacctcc tcgatgaggg
      841 tccgatgccc ggcccggatg gccccgatcg cctttcccgc ctcccggacg tcctcctcgc
      901 cgaagtagat gccgttggtg agatggatcc tgtggtcggg ggtggcgaca tagggcagct
      961 tgatcacccc cgtctccagg cccaatgcca tcgcggcgac cgtcccggtc ccggtgatgc
     1021 cccgggcgac caccccgtcc aagcgccgct cctcaccggt ggtgggatcc acgagagagg
     1081 acttgactgg gtgaaggttg tcgttgagca cgatattgta ccatttaccg tcgtccgcca
     1141 aggccaggtc ggaaatggcc tgcggagcgg ccagcatgcc gaagtctatg gactgcccct
     1201 ccatggccgg gcccgcggcg gcggagcctg tgtagagttc gcccttgtgg aacagcccca
     1261 tctcggcgtt ggtcccatag tccgtgacca tgcacgtctc gttcttctcc atgagcccgg
     1321 tcttcatgac cattgcgagc gcgtcggcgc ctatctcgtg ccggatggac ggggggatgc
     1381 gcacctcggc ggtgccctta acggacctca tccccagctc ctcggccttg atggtatggg
     1441 ccttcctctc cggcaacttg acgttgagcc tcttgaggat cgactgcccc gcgaaggcca
     1501 ggtcgcggat ctctatcttc tcgaacatag acatctgggc ggggttgccg caaacggaca
     1561 cccggatgat ctcctcgggc ttggcgccca tggtcccaat gagcttgtct acggtctcga
     1621 tgatgata
//
LOCUS       DAOSYN010000281         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_56047_length_1621_cov_2.789911, whole genome shotgun sequence.
ACCESSION   DAOSYN010000281 DAOSYN010000000
VERSION     DAOSYN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56047_length_1621_cov_2.789911"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..514)
                     /locus_tag="PLJ11_08050"
     CDS             complement(5..514)
                     /locus_tag="PLJ11_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04655.1"
                     /translation="MQPRVRSALLALAIMFVLVSTAYLAAGALHDRLAPEPEEPVADV
                     RLVAVAIGGLGGEEERSPLLATLNVGGEYDIGIWVQGLRSEGGVRTMFSLECEGIDKG
                     DVDVYYYDPGSGSWKPVAMVDRGDSLQGIVGPPNGVAVYYGYDQLYRLLIISHIDGKC
                     TPDAWVEWG"
     gene            complement(572..1036)
                     /locus_tag="PLJ11_08055"
     CDS             complement(572..1036)
                     /locus_tag="PLJ11_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOO04656.1"
                     /translation="MLEVEVLDGLRGAGLTIAIAESCTGGRICDRLTDVPGSSERFMG
                     GVVAYSNASKVSLLGVSPSTLKASGAVSEECAREMAAGVAKAFGADMGVAVTGIAGPG
                     GGTPEKPVGLVFIAIARKGERAAFRMVFDGDRERVKAQATEEALRLTLSFIP"
     gene            complement(1029..1565)
                     /locus_tag="PLJ11_08060"
     CDS             complement(1029..1565)
                     /locus_tag="PLJ11_08060"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HOO04657.1"
                     /translation="MSDPKRVDGLLIGRFQPFHLGHLEVVRKIARECDELIIGIGSAQ
                     ISHTLDNPFTAGERHLMISRALREDGIRDFFLVPIVDINQYGIWVSHVRSMVPPFERV
                     YTNNPLTKRLFREAGYEVSASPMFNRSQYSGTEIRRRMIAGDDWRGYVPRAVAEVIDS
                     IDGVRRLRDLAGKGEPDA"
BASE COUNT          265 a          577 c          519 g          260 t
ORIGIN      
        1 gggctcagcc ccactccacc caagcatcgg gcgtgcattt cccgtctata tgcgagatga
       61 tgagcaatct gtagagctga tcgtaaccgt agtacaccgc caccccgttc ggggggccta
      121 caatgccctg gagggaatcg cccctgtcca ccatggcaac gggcttccag gaaccgctgc
      181 cgggatcgta gtagtaaacg tccacatcgc ccttgtcgat gccctcgcat tccagggaga
      241 acatggtccg gaccccgccc tcgctgcgaa ggccctggac ccagatcccg atgtcgtatt
      301 cgccgccgac gttcagcgtc gccaggaggg gcgaacgctc ctcctcccct ccgagcccgc
      361 cgatggccac ggccaccagg cgaacgtcgg cgacgggctc ctccggctcc ggggcgaggc
      421 ggtcgtgcag cgcccccgcg gcgaggtagg ccgtggagac gagcacgaac atgatcgcca
      481 aggccaggag cgccgagcgg acgcggggct gcatcgcacc atcaccttgg gacagatggc
      541 catccggcgg atatatcggt ttgccccggc gtcaggggat gaaactcagg gtgagccgca
      601 gggcctcctc ggtcgcctgc gccttcaccc gttcccggtc cccgtcgaag accatcctga
      661 acgccgccct ctcccccttc cgggcgatgg ctatgaagac gagcccgacg ggcttctccg
      721 gcgtcccgcc ccccgggccg gcgatgccgg tgaccgcgac gcccatgtcc gccccgaacg
      781 ccttggcgac gcccgcggcc atctccctgg cgcactcctc gctgaccgcc ccggacgcct
      841 tcagggtgga gggcgagacc ccgaggaggc tgaccttgga ggcgttgctg tacgccacca
      901 cgccgcccat gaagcgctcc gaggagcctg ggacgtcggt caggcggtcg catatgcgcc
      961 cgccggtgca cgactcggcg atggcgatgg tgagcccggc ccccctgagc ccgtccagca
     1021 cctcgacctc aagcatccgg ctcccccttc ccggcgaggt cccggaggcg cctgacgccg
     1081 tcgatcgagt cgatcacctc cgcgaccgcc cggggcacgt atccgcgcca gtcgtccccg
     1141 gcgatcatgc gcctgcggat ctccgtcccc gagtactgag agcggttgaa catcggggag
     1201 gctgacacct cgtaccccgc ctccctgaac agcctcttgg tcagcggatt gttcgtgtac
     1261 acgcgctcga acggggggac catcgagcgc acgtgcgaca cccagatgcc gtactggttg
     1321 atgtcgacga tgggcacgag gaagaagtcc cggatgccgt cctcccgcag ggcgcgggag
     1381 atcatgaggt gccgctcgcc ggcggtgaag gggttgtcga gggtgtgcga gatttgcgcg
     1441 ctcccgatac ctatgatgag ctcatcgcat tccctggcga tcttccgcac cacctccagg
     1501 tgcccgaggt ggaagggctg gaaccgcccg atcagcaggc cgtccacgcg cttggggtcg
     1561 ctcatagcgc cccttcaatc cggttcaggt gataaaatcc ttgtcattgg gaagggggga
     1621 g
//
LOCUS       DAOSYN010000282         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_56482_length_1611_cov_3.438946, whole genome shotgun sequence.
ACCESSION   DAOSYN010000282 DAOSYN010000000
VERSION     DAOSYN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56482_length_1611_cov_3.438946"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLJ11_08065"
     CDS             complement(<1..370)
                     /locus_tag="PLJ11_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04658.1"
                     /translation="MYRFQMEQRVLSVGDVRVGGQPGKKRTMLVGSLFYPRHSVVLDP
                     RRGTLDHARLTDRLDRYLEAVRACACPSALMLYAESEEATRPYLEAVCDRVTGPLFAD
                     AGTFQVRSAFLRNAEEMGLLD"
     gene            complement(372..1139)
                     /locus_tag="PLJ11_08070"
     CDS             complement(372..1139)
                     /locus_tag="PLJ11_08070"
                     /inference="COORDINATES: protein motif:HMM:NF016124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04659.1"
                     /translation="MEGTNAWPVVSGDYVLGNEASPVAVLIVGRGAVDVPKDLFCIKG
                     ILKTENIGLEKVIANIVSNPRIRLLVLCGREEFGHFPGEAIHALMAHGVDEHKRIRNT
                     RSAIPFLCDLPFEAIERFRQQVELVDIMDASPAKEIAAYDPIYEFDEEGRGRLVSTLR
                     TLAEQPLEPFPEEAVIVRSKALTGEGGSIATQLHLASDDFITPMLRLPSDGLNTGMGM
                     VLVSEEFGVVLEPLRGKVLTVPSVELALRLRSYLMGV"
     gene            1298..>1611
                     /locus_tag="PLJ11_08075"
     CDS             1298..>1611
                     /locus_tag="PLJ11_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04660.1"
                     /translation="MGVSMAICELDSDSALCKAGKAQLLKVHLKDVSGFEDLAEYDIV
                     VPLDRATQVLGAEWEAFLKRNRLDPEVGMVYLDKIKNEADRQLLAAEGRKRYTGWIAL
                     DKV"
BASE COUNT          331 a          509 c          487 g          284 t
ORIGIN      
        1 ggtccaggag ccccatctcc tcggcgttcc tgaggaaggc cgatcggacc tggaaggtcc
       61 cggcatcagc gaaaagcggc cccgtgacgc ggtcgcagac cgcttcaagg tatggtcggg
      121 tggcctcctc gctctccgcg tacagcatca gggcggaggg gcaggcgcag gcgcgcacgg
      181 cctcgaggta gcggtccagt cggtccgtta accgagcatg gtccaatgtc ccccggcgag
      241 ggtcgaggac gaccgaatgc cggggataga aaagggaacc aacaagcatc gtccttttct
      301 taccgggctg ccccccgacc cgcacgtccc cgacgctcag gacccgctgc tccatctgga
      361 accggtacat gtcacacccc catgagatag gagcgcaatc gcaaggccag ctcaacggac
      421 gggacggtca gcaccttccc tcgcaggggc tctaggacca ccccgaactc ctcggagacc
      481 aggaccatgc ccattcctgt gttcagccca tccgagggca ggcggagcat aggggtgatg
      541 aaatcatccg aggccaggtg caactgggtg gcaatgcttc ccccctcacc ggtaagggcc
      601 ttggagcgga ctattacagc ctcctccggg aacggctcaa ggggctgctc cgcaagtgtc
      661 ctcaacgtgc tcaccaaccg cccccgccct tcctcgtcga actcatagat agggtcgtac
      721 gccgcgatct ccttggccgg agaggcgtcc atgatgtcca ccagttccac ctgttgccgg
      781 aagcgttcga tggcctcgaa cggaagatcg cacaggaagg ggatggccga tctggtgttc
      841 ctgatgcgtt tgtgctcgtc gaccccgtgg gccatgagcg cgtggatcgc ctccccggga
      901 aagtgcccga actcctccct gccgcagagg acaaggagcc ggatgcgcgg gttggagacg
      961 atgttggcga tcaccttctc gagcccgatg ttctctgtct tcagtatgcc ctttatgcag
     1021 aagaggtcct tcgggacgtc caccgccccc ctgcccacta tgagcaccgc caccggcgag
     1081 gcttcgttcc ccaggacgta gtccccactg accaccggcc acgcgttcgt cccttccata
     1141 gtcgctccca gtacaaatcc gatttcaaga atatatagaa cgtccccttg tcacgttccc
     1201 ttaaggacca tcgggtcccg caagccccaa tttcaacgtt acgaagggaa gcgataaata
     1261 cagccctttt tgtactacgc ggcagaaggg atgtaaaatg ggagtctcaa tggccatatg
     1321 cgaactggac agcgacagcg ccctatgcaa ggcgggcaag gcccagctgc tcaaggtcca
     1381 cctgaaggac gtctcgggct tcgaggacct cgcggagtat gatatcgtgg tgccgctcga
     1441 ccgggccacg caggtccttg gcgcggaatg ggaggcgttc ttgaaaagga acaggctgga
     1501 cccggaggtg gggatggtgt acctggacaa gatcaagaac gaggcggacc ggcagttgct
     1561 ggccgccgaa ggccgaaagc gctacaccgg ctggatagca ttggacaaag t
//
LOCUS       DAOSYN010000283         1605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_56762_length_1605_cov_3.331613, whole genome shotgun sequence.
ACCESSION   DAOSYN010000283 DAOSYN010000000
VERSION     DAOSYN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56762_length_1605_cov_3.331613"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>1605
                     /locus_tag="PLJ11_08080"
     CDS             196..>1605
                     /locus_tag="PLJ11_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOO04661.1"
                     /translation="MLGRRSLLLFLSRMVSSALAYIGLYFMTRYLGTEIYGTVTYTMA
                     FVATFNAVSDLGFGSAHVKKVSEGEDPNACISTYAAIKLILTGAMVVLVLTSVFIWTG
                     PMGHTLSGTSIDLILLFVLYQVLYDLSAIATTTFQARMEMAKMPLVTLIDPLVRVPLI
                     IFVSVNRMGVWELALAYVIGAIAVALLALFLLFRERVRWQRPVLMRQYFNFALPLILI
                     TIISTVSGSADKLIIGAFWTASDVGLYTAPAVFLGVFATVSTAVSTLTFPSFSKLHAE
                     GNLAEIREMSKQAERYIAMISLPITVLIIMFPYEVCLILLGPLFIESGDAIAIMAVTN
                     FLTMMNAVHGSQIVAVGRPDISARITILTVAINISLMLLFIPQYGLGLSYVGAALALM
                     IGNIIGFIMVRYAVWRLTRTPPNPRLALHLVAGAAAALVMFALDMIISVDRFYTLILF
                     GLVAFAAYLPVLALVGEFKR"
BASE COUNT          253 a          542 c          481 g          329 t
ORIGIN      
        1 ccgctcacgt agaagagcca ggggccgagg gacgccatgg cgcatgggaa tatggagggg
       61 gcgtacccca acccggccat ggtggccatc gtcactgtgg acctgatgat cggagctgct
      121 ctgattgcca ggggggaggt gggtgagggg ctggcgcagg gttaaatagc cccgcgcatc
      181 tctccagccg attacgtgct aggcagacgc tccctcctgc ttttcctttc ccggatggtc
      241 tcttcggccc tggcctatat cgggctgtac ttcatgaccc ggtacctggg cacggagatc
      301 tacgggacgg tcacctacac catggccttc gtggccacct tcaatgcggt gtccgacctg
      361 gggttcggga gcgcgcacgt caagaaggtc tcggagggag aggacccgaa cgcgtgcata
      421 agcacctacg cggccatcaa gctcatcctg accggggcga tggtggtcct cgtcctcacc
      481 tccgtattca tctggacggg gccgatgggg cacaccctca gcggcaccag catcgacctc
      541 atcctcctgt tcgtcctgta ccaggtgctg tacgacctct cggccatagc caccaccacc
      601 ttccaggccc ggatggagat ggccaagatg ccgttggtca ccctgatcga tcccctggtc
      661 cgggtgcccc tcatcatctt cgtctccgtc aaccgcatgg gggtctggga gctcgcgctc
      721 gcctacgtca tcggggccat cgccgtcgcc ctcctcgccc tcttcctgct gttccgggaa
      781 agggtgcggt ggcagaggcc cgtgctcatg cgccagtact tcaacttcgc cctgccgctc
      841 atcctgatca cgatcatcag caccgtgagc gggagcgcgg acaagctgat aatcggggcc
      901 ttctggaccg cctccgacgt cggcctgtac acagccccgg cggtgttcct cggggtgttc
      961 gccaccgtga gcaccgcggt gtccacgttg accttcccct cgttctccaa gctgcacgcc
     1021 gaggggaacc tggccgagat ccgggagatg tcgaagcagg ccgagcggta catcgccatg
     1081 atcagcctgc ccatcacggt cctgataatc atgttcccct acgaggtctg cctgatcctg
     1141 ctcgggccgc tgttcatcga gtccggggac gcgatcgcga tcatggcggt gaccaacttc
     1201 ctgacgatga tgaacgcggt gcacggctcg cagatcgtgg ctgtgggccg accggacatc
     1261 tccgcccgca tcaccatcct caccgtggcc ataaacatct ccctcatgct cctgttcatc
     1321 ccgcagtacg gcctcggtct ctcgtacgtg ggggcggccc tcgccctgat gatcggcaac
     1381 atcattgggt tcataatggt ccgctacgcg gtgtggcgcc tcaccaggac ccccccgaac
     1441 ccccgcctcg ccctccatct cgtggccggg gcggcggccg cgctggtcat gttcgctctg
     1501 gacatgatca tctctgtcga tcgcttctat acgctcatcc ttttcgggct ggtcgccttc
     1561 gcggcgtacc tgccggtgct ggcgttggta ggggagttca aaagg
//
LOCUS       DAOSYN010000284         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_56994_length_1600_cov_3.212298, whole genome shotgun sequence.
ACCESSION   DAOSYN010000284 DAOSYN010000000
VERSION     DAOSYN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56994_length_1600_cov_3.212298"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLJ11_08085"
     CDS             <1..562
                     /locus_tag="PLJ11_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04662.1"
                     /translation="EVREIAIKSSRALIRMASGVVHGMHKGSDCTPELAEAIDEAHRL
                     KSVLSEHVDIWHSGMVQDALVEVAEAALLNAIVKGQELPDPTRLGMSGTAYLMGMADT
                     IGELRRFALEALRRGDIPSAERYLDIMEELFLVIMRFDYPDAIVSIRRKQDIARSILE
                     KTRGDVAIAAMTERLQERMDQLERRL"
     gene            complement(563..1330)
                     /locus_tag="PLJ11_08090"
     CDS             complement(563..1330)
                     /locus_tag="PLJ11_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOO04663.1"
                     /translation="MVALMAMVLLAALPGVQGEEYGPLYAHIEGPLVVSTGSVTMYTL
                     EMVGGPAEVGAGNYSYKATMTGDGDTYGAFFLPGTVEPSPDGTFHINLTAPSAAQTLT
                     ISIECSSAGAVTVKSTITLKVKVVEPIVLKATVRNTGEVAAIGVPLSLQVYEGGEWRE
                     FYSTTVDLAAGEAYAFQYNWTAIGLGSGEHKVRMVLDPGDRIVAFEGGSTVYGTTIYY
                     DMPGYGWANTLMGVLVVALALVLIYVWRRPAKGKRKR"
     gene            complement(1330..>1600)
                     /locus_tag="PLJ11_08095"
     CDS             complement(1330..>1600)
                     /locus_tag="PLJ11_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04664.1"
                     /translation="PLRANPDPKASVQLVAVSTADPAVQEVMEFQPDLVGLSASGLSV
                     SGAGVSGSAPGLGSTAIALMGVAIALAVALVVVSVQKGVFSRRKR"
BASE COUNT          263 a          544 c          536 g          257 t
ORIGIN      
        1 cgaggtgcgg gagatcgcca tcaagtcctc ccgcgcgctg attcgcatgg ccagtggggt
       61 ggtccacggt atgcacaagg gatcggattg caccccagag ctcgccgagg ccatcgacga
      121 ggcgcaccgg ctcaagagcg tgctctcgga gcatgtggac atctggcact ccgggatggt
      181 gcaggacgcc ctcgtggagg tggccgaggc ggccctcctg aacgccatag tgaaggggca
      241 ggaactcccg gaccccaccc gtttgggcat gtccggcacc gcctacctga tgggcatggc
      301 ggacaccatc ggcgaacttc ggcggttcgc attggaggcc ctgcggcggg gggacatccc
      361 ctccgccgag agatatttgg acatcatgga ggagctcttc ctggtcatca tgcgcttcga
      421 ctatcccgac gccatcgtct ccatccggcg caagcaggac atagcccgct ccatcctgga
      481 gaagacccgg ggggacgtgg cgatcgccgc gatgaccgag cggctgcagg aaaggatgga
      541 ccagctggag cggaggctgt gatcaccgtt tcctcttgcc cttggccggg cgcctccaga
      601 cgtagatgag cacgagggcg agcgccacga cgagcacgcc catcagggtg ttggcccagc
      661 cgtagccggg catgtcgtag tagatcgtgg tcccgtagac ggtcgagccg ccctcgaagg
      721 cgacgatccg gtccccgggg tccaggacca tgcgcacctt gtgctccccg gagccgaggc
      781 cgatggcggt ccaattgtac tggaaggcgt acgcctcccc ggcggccagg tcgacggtcg
      841 tgctgtagaa ctcccgccat tccccgccct cgtacacctg gaggctgagc gggacgccga
      901 tcgcggccac ctcgccggtg ttccggacgg tcgccttgag gacgatcggc tccacgacct
      961 tcaccttgag ggtgatggtg ctcttcaccg tcacggcgcc cgcggagctg cactcgatgc
     1021 ttatcgtaag ggtctgcgcg gcgctggggg cggtcaggtt gatgtggaac gtcccgtcgg
     1081 gggatggctc caccgtcccg ggcaggaaga aggccccgta ggtgtccccg tccccggtca
     1141 tggtggcctt gtaggagtag ttgcccgctc ccacctccgc cggcccgcca accatctcca
     1201 gcgtgtacat ggtcacgctg cccgtcgaga cgacgagcgg gccttcgata tgcgcgtaca
     1261 gcggtccata ttcctccccc tggacgcccg gcagggcggc aaggagcacc atcgccatga
     1321 gcgcgaccat cagcgctttc ttcgtgagaa cacccccttc tgcaccgaga cgaccacgag
     1381 cgccaccgca agggcgatcg caacccccat gagggcgatg gcggtgctgc ccaggcccgg
     1441 ggcggagccc gagacgccgg ccccgctcac gctcaggcca ctggcgctca ggcccaccag
     1501 gtcgggttgg aactccatga cctcctgcac ggccggatcg gccgtggaca cggccaccag
     1561 ctgcacgctc gccttggggt ccgggttggc ccgcagaggc
//
LOCUS       DAOSYN010000285         1594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_57234_length_1594_cov_4.212476, whole genome shotgun sequence.
ACCESSION   DAOSYN010000285 DAOSYN010000000
VERSION     DAOSYN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57234_length_1594_cov_4.212476"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..881
                     /locus_tag="PLJ11_08100"
     CDS             465..881
                     /locus_tag="PLJ11_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO04665.1"
                     /translation="MNRQIERKKVKDVMTTGVITVSPETDIRTLKEMFERYDFNSFPV
                     VKDGKVVGIVSKLDLLKAFTAGLNVSLGRMLKLYSKSASDIMHTAIISVGPEDDVSTA
                     ADYMVEYQIRSMPVIEKGKLKGMISREDIIRCLRIE"
     gene            complement(856..1488)
                     /locus_tag="PLJ11_08105"
     CDS             complement(856..1488)
                     /locus_tag="PLJ11_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOO04666.1"
                     /translation="MIIDTINQFIIDLIGTTGYAGVFLAMMIEGIFTPIPSELIMPFA
                     GYVAYTGELMFFLVILIGSIGAMVGSFIAYMLALKLGRPLIDRYGLLFGLDQEKMGAA
                     ERWFERWGVWGIFIGHSLPGIRSIISFPAGLAKMDRTKFAGFTFLGALVWNTVLVTAG
                     YTLGDEWIRFWESTDGLDYIVLGAAGAVLVAYLLYRRWKKPKLTRSADSG"
BASE COUNT          367 a          435 c          445 g          347 t
ORIGIN      
        1 tgcaccggtc cgtttttcaa tgggttcccg ataaggacgg cccgctgatc tctcgggacc
       61 cttcatcgga tttactgatc tatttgctgg tcccgttttg ttattccaag cataggtaag
      121 aacctggaca tcgttgtcca ttcagccagt tcgatgttca cggacattca ttcgagggtc
      181 ctggaaacgc catcccgatc ccatccccgt tacctagaac ggcttgtcag gaacatcccc
      241 tccttacctg accggtttca gcccagcagg atcgaactgg acaaaacctt agtgcagtct
      301 gctgggaaga tgggctggga gcgaggggtg ggaatatgag gatcgtccca tcccaccgct
      361 gaccgttgtt cttggaggcc attccgctgc gtcctatccg ctcccgaaga gcattgttgc
      421 gatagttgtt tatgggccca tggtcctatt taataggagg accgatgaac cgccagatcg
      481 aacgtaaaaa ggtgaaggac gtgatgacca ccggagtgat caccgtatcc cccgagaccg
      541 acatccgcac cctcaaggag atgttcgaac gatacgattt caacagcttt ccggtcgtga
      601 aggacggcaa ggtcgtaggg atcgtctcca agctggacct gttgaaagcc ttcaccgcgg
      661 gattgaacgt gagccttggt cgcatgttga agctctacag caagagcgct tcggacatca
      721 tgcacaccgc tatcatctcc gtcgggccag aggatgacgt ctccacggcc gccgattata
      781 tggtggaata tcagatacgc agcatgccgg tcatcgaaaa gggaaagctg aagggaatga
      841 tctcccggga ggacatcatc cgctgtctgc ggatcgagtg agcttgggct tcttccatct
      901 gcggtagagg aggtaggcca cgagcaccgc gcccgccgcc cccagcacga tatagtcgag
      961 gccgtcggtg ctctcccaga acctgatcca ttcgtcccca agggtatacc cggcggtgac
     1021 caggaccgtg ttccatacca atgcgccgag gaaggtgaag cctgcgaatt tcgtgcggtc
     1081 catcttcgcc agccctgctg ggaaggagat gatcgagcgg atgcctggaa gggaatgccc
     1141 gatgaatatg ccccagaccc cccagcgctc gaaccagcgc tcagctgccc ccatcttctc
     1201 ttggtcgagg ccgaagagga gcccgtagcg gtcgatgagc ggtctgccta gtttgagggc
     1261 gagcatatac gcgataaagg agccgaccat ggccccgatg cttccgataa ggatgacaag
     1321 gaagaacatc agctccccgg tataggcgac atacccggcg aacggcatga tcaattcgct
     1381 tgggatgggg gtgaatatcc cttctatcat catcgccagg aatactcctg cgtatcctgt
     1441 tgtgccaatg aggtcgatga tgaactgatt gatcgtgtcg attatcatgc gcccaccaag
     1501 cgtcgaacga gtgagttaat gctcccttat ataaaaaagg aacaggacct agattgaaga
     1561 tgggtctaga cagaagggcg attcgatttg tatc
//
LOCUS       DAOSYN010000286         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_57878_length_1581_cov_2.693316, whole genome shotgun sequence.
ACCESSION   DAOSYN010000286 DAOSYN010000000
VERSION     DAOSYN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57878_length_1581_cov_2.693316"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1581)
                     /locus_tag="PLJ11_08110"
     CDS             complement(<1..>1581)
                     /locus_tag="PLJ11_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO04667.1"
                     /translation="SCRANGSFIPVHASVYTSDEGYAPKNYCLACGSYSRKEKPVLVE
                     ARAVQVADVHILAQEMIQAMPKAHRKLLVFADSRQDAAFQAAWMQDHARRYRMRQLFY
                     NVLLGQGPMTTGDAVARIIEALDREPSLQEVIAPDLIEKHHGNIEGQEFLRDRKRYFH
                     IHFLREVCEGAGRIDTLENWGLLGVGYSGLGEDHEIVQGLSERFSLDPAAVLDFILGF
                     LDYWRTRGFLHSPATLLYSRAWDDTMAEVRDGWLSKNSMYPRALVLESAKDLPNSRIK
                     GVMSSRGQTWSMQFAKKAFPQLEGVQSLLSEVWGRLIEKGYLVEGEIGQTDSGRRGGR
                     RTFGGFQINEEKLRVCTQRELYVCERCGKVASRQTPGLICPSWNCYGTMRREEPSEEV
                     YDVSVLMNPDFNVMVPREHSAQVPHQLREKVEREFKQGKGVNCIVATPTLELGIDIGD
                     LDGILMRNMPPDPSNYWQRAGRAGRRQRMAVIYTYCRNTHHDLAYFERPLEMLGGRIS
                     PPRINLRNKHMVTRHVHGT"
BASE COUNT          281 a          507 c          454 g          339 t
ORIGIN      
        1 ggtcccatgc acatgccggg tgaccatgtg cttgttgcgg agattgatcc tcggaggcga
       61 tatccttccg ccgagcatct ccaggggcct ctcgaaatac gcaaggtcgt gatgggtgtt
      121 ccggcagtag gtgtagatca cggccatcct ttgcctcctg ccagctcttc ccgccctctg
      181 ccaatagttc gaagggtcgg gaggcatgtt cctcatgagg atgccgtcca ggtccccgat
      241 gtcaattcca agctccaatg tgggggtggc gacgatgcag ttcactccct tgccctgttt
      301 gaactccctc tccaccttct cccgtagctg atgcgggacc tgcgcagaat gttcccgggg
      361 caccatgacg ttgaaatcgg ggttcatcag cacggagacg tcatagacct cttccgaggg
      421 ctcctccctt ctcatcgtcc catagcaatt ccaggaggga cagatgaggc ctggcgtttg
      481 gcgggaggcg acctttccgc acctttcgca tacatacagc tcccgctggg tgcagaccct
      541 gagcttctcc tcgttgatct ggaatccgcc gaacgtacgg cgtccgcccc tccgtccgct
      601 gtccgtctgt cctatctccc cttccaccag atagcccttc tcgatcaatc tgccccacac
      661 ctcgctcagc aggctctgta cgccctcgag ctgcgggaac gccttcttgg cgaactgcat
      721 ggaccaggtc tgccccctag aggacatcac cccttttatc ctggaattgg gaaggtcctt
      781 cgccgattcc aataccaatg cccgggggta catcgagttc ttgctcaacc agccgtcgcg
      841 cacctctgcc atggtgtcat cccatgccct ggaatagagc agggtggcgg gggaatgaag
      901 gaagcccctg gtccgccaat agtccaggaa gccgaggatg aaatcaagga cggcggcggg
      961 atcgagcgag aaccgctcgg aaagcccctg tacgatttcg tggtcctcgc ccagaccgga
     1021 gtagccgacc cccagtaggc cccagttctc cagggtgtcg atgcgccccg ccccctcaca
     1081 cacctcccgc aggaagtgga tatggaaata ccgcttgcgg tcgcgcagaa actcttggcc
     1141 ctcgatgttg ccatgatgct tctcgatgag gtcgggggcg ataacctcct gcaaactcgg
     1201 ttccctgtcc aaagcctcta ttattctagc cacggcgtcc cccgtggtca tcggcccttg
     1261 gcctaggagc acgttgtaga agagctgcct catgcggtag cgccgggcat ggtcctgcat
     1321 ccaggcggcc tggaaggcgg cgtcctggcg gctgtcggcg aagaccagga gcttgcgatg
     1381 tgccttgggc atggcctgga tcatctcttg ggctaggatg tggacgtccg ccacctggac
     1441 cgccctggcc tcgaccagca cgggcttctc cttcctggag taggagccgc aggccaggca
     1501 gtagttcttg ggcgcatacc cttcgtccga ggtgtatacg ctcgcgtgta ctggtatgaa
     1561 ggagccgttg gcacgacacg a
//
LOCUS       DAOSYN010000287         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_58030_length_1578_cov_3.485227, whole genome shotgun sequence.
ACCESSION   DAOSYN010000287 DAOSYN010000000
VERSION     DAOSYN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58030_length_1578_cov_3.485227"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLJ11_08115"
     CDS             <1..192
                     /locus_tag="PLJ11_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethyl-7,8-dihydropterin
                     pyrophosphokinase"
                     /protein_id="HOO04668.1"
                     /translation="KNVYNFGGFTDGDRAFFMAKHFEAKEVRFLGWDLDRPSPKRGRD
                     PSLKERKLRWAKRLLQPYI"
     gene            498..>1578
                     /locus_tag="PLJ11_08120"
     CDS             498..>1578
                     /locus_tag="PLJ11_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO04669.1"
                     /translation="MEGSADAEPKKVGGSKIIAIFIVGLLVGAALGAGIMMVLGGGED
                     EETVYKTVVVATIGGNPESLDPAYDYETVGGEIIQNVYETLMFYDGGSAEVLVPQLCT
                     EVPTIENGLITDDGHIYTYNLREGVTFHDGTVMTSEDVFYSFARVLRLNDPHGPAWMV
                     GELLIPNYYDYVWANNRTIATEFTSQDLKDKLVVPLYPAAEFDSSGIIKEYTGVSMDT
                     IREHIWAKDDYTVQFNLTVAFPGWNYVITFGVGSIISKDYVELHGGMTKAGYDYMFNH
                     MCGTGAFSLEEYTPDSQFVLLRYDDYWKGPAKINKVIIMQVPDDNTRLLLLKSGQVDI
                     AAIPRTLRNSVVGDANIEITEGLPTF"
BASE COUNT          442 a          318 c          417 g          401 t
ORIGIN      
        1 aagaacgtct acaacttcgg ggggttcacc gacggggacc gcgccttctt catggccaag
       61 catttcgagg ccaaggaagt acggttcctt ggatgggacc tggacaggcc gtccccgaag
      121 cggggacggg acccctccct gaaggaaagg aaattgagat gggccaagcg gcttcttcaa
      181 ccctatatat aatttttcca ggtgaattgg aggtcggtcg atcagggcct gatgaacagg
      241 aatggccgaa caggcctaga gaggtaataa tacaatgtga caatctatcc cttctatttg
      301 ggttcatagt gtcaaataaa ttggaatttt gttagacaca aaatatgata ataaggcggt
      361 tcatcggacc ctccattcgt aatactggac aatgctggtt gggcctggaa aatcgacaaa
      421 ggtatatata gcgacaaaat gtttaaagaa gtcaatctcc cagtcgttca cggcgtggga
      481 ggaaaggagg attgagaatg gaaggaagtg ctgatgctga acctaaaaag gtcggtggtt
      541 ccaaaatcat cgcgatcttc atagtggggc tcttggttgg tgccgcactt ggagctggaa
      601 tcatgatggt cttaggggga ggcgaggatg aggaaacggt ttataagacc gttgttgtag
      661 ccacaatcgg tgggaatccg gaaagcctgg accccgctta tgactatgaa actgttggcg
      721 gggagattat tcaaaatgtg tatgagaccc tgatgttcta tgatggcggc agtgcagaag
      781 tacttgtacc gcaactttgc accgaggttc cgacaataga aaatggttta attaccgacg
      841 atggacatat atatacctac aacctgcgtg agggtgtcac attccacgat ggtactgtaa
      901 tgacctctga ggatgtgttc tacagcttcg ctagagtcct gaggttgaac gacccacatg
      961 gtcccgcatg gatggttggt gaactgttaa tcccgaatta ctatgattat gtctgggcga
     1021 acaatagaac cattgcaaca gaatttacat ctcaagattt aaaggataaa cttgttgttc
     1081 cgttgtaccc agcggctgag tttgactcct ctggcattat aaaggaatat acgggggttt
     1141 caatggatac tatccgtgaa catatttggg caaaagatga ttacacggta caattcaatt
     1201 tgaccgttgc cttcccagga tggaactatg tgatcacatt cggagtaggt tctatcattt
     1261 cgaaggacta cgtagaactt catggtggca tgacaaaggc tgggtatgat tacatgttca
     1321 accacatgtg tggaactgga gcgttctcat tagaagaata taccccagat tcacagtttg
     1381 tgctgctccg gtatgatgat tattggaaag gaccggcaaa gatcaacaag gtcattatca
     1441 tgcaggtacc tgatgataat acaaggttgc tcctgcttaa gagcggccag gtagacattg
     1501 ccgccatacc caggaccttg agaaattctg tcgtgggcga tgccaacatt gaaataactg
     1561 aagggctccc aacgttca
//
LOCUS       DAOSYN010000288         1557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_59104_length_1557_cov_3.503329, whole genome shotgun sequence.
ACCESSION   DAOSYN010000288 DAOSYN010000000
VERSION     DAOSYN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59104_length_1557_cov_3.503329"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(364..720)
                     /locus_tag="PLJ11_08125"
     CDS             complement(364..720)
                     /locus_tag="PLJ11_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO04670.1"
                     /translation="MKVLETSRLLTEEYTAKILMATMGRPKSAFELSDALGIPIAACY
                     RKIRVLEEAGLIRCCERRLTQSGKRMSMYKSMVRNANITFERNRIRARIEMEDGSSTD
                     DCFDIDLGVMFKETHA"
     gene            complement(1156..>1557)
                     /locus_tag="PLJ11_08130"
     CDS             complement(1156..>1557)
                     /locus_tag="PLJ11_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO04671.1"
                     /translation="LVLLSAMAIGVSVVLSRIALRTLSPEQWSLGLHLLLPLALLALY
                     FLVPMDGGLGAGALPGVLFIGLVCTTVPTVLWTTGLRHLSTVTSATVLLVESAFAVLL
                     SWLLLGESMDVYVLAGAAMIFLAILLMARSD"
BASE COUNT          374 a          444 c          408 g          331 t
ORIGIN      
        1 acatctcccg gtactgctcc atgctcttga tgtaggcctt ctcgctgaac tcctttgagg
       61 gcgggaaccg ccgcacctcc tccatcacgc tctcaatggt cttgccgtcg ctcatcagtc
      121 gctcctcctg tcggcgtaaa tccgccggaa ccgaacggat cgcagccctc cttccgtagg
      181 attattaata aagattatgg aaaacgtata ggacaagcgg aattaattat aatttaccac
      241 tggtcctact gatattgtcg atttttttac gtattgcgat tccattacga aaggaatgag
      301 tccaatgaga attagcccgc agcgatagag ggccgtcgaa ggaaaaggcg cgccaagggg
      361 gtctcaggcg tgggtctcct tgaacatcac cccgaggtcg atgtcaaaac aatcatcggt
      421 actgctgccg tcctccatct cgatgcgggc gcggatccgg ttgcgctcga aggtaatgtt
      481 ggcgttgcga accatggact tgtacatgct catgcgcttc ccggactgtg taagccgccg
      541 ttcacagcag cggataaggc cagcctcctc cagcaccctg atcttgcggt agcaggccgc
      601 tatggggatc cccagcgcgt cgctgagttc gaaggcgctc ttggggcggc ccatggtagc
      661 catcaatatc ttggctgtat actcctcggt cagcaggcgc gacgtttcca ggaccttcat
      721 cgttcccaca aaagaattct accgctcaga gtaatatata atcaaaatct tcatagaatc
      781 acttttgata ttgaacgaat gcgcccggcc agcatacggg atgaaggtca tcatgaaggg
      841 cgttttatcc gctctggtaa tcaacgttgt cttacagcgc tactttggag gtagcaaaat
      901 ttccgataac gtattacttt taacctactg aattaacatt tatattactc attggataag
      961 attgaaatat agtccgagcg atcacttccc catgggtctc ccccatagga ggtagaacat
     1021 gtcctgtgga aagtgcggca cgaagggtga aaagaagaag gaggaaaaga agaagtgatt
     1081 cggcaacgtc ttctcaaaac cttcttaaaa ccctttcctg cctttctatt tctcattgag
     1141 gaccgcccct aacgctcaat cggacctggc catcaacagg atggcgagga agatcatcgc
     1201 cgccccagcg agcacgtaga catccatgct ctccccgagc agcaaccagc tcaacagcac
     1261 cgcgaaggcc gactcgacca gcaggaccgt cgccgaggtc accgtgctca ggtgccgcag
     1321 ccccgtcgtc cagagcacgg tgggcacggt ggtgcacacc aggccgatga agaggactcc
     1381 cggcagagcc cctgccccca ggcccccgtc catcggcacc aggaagtaca acgcgagcaa
     1441 tgcgagcggg aggagcaggt gcagccccag gctccactgt tccgggctca gggtccgcag
     1501 cgcgatgcgc gagaggacca ccgaaacgcc gatggccatg gcggagagga ggaccag
//
LOCUS       DAOSYN010000289         1544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_59776_length_1544_cov_3.887844, whole genome shotgun sequence.
ACCESSION   DAOSYN010000289 DAOSYN010000000
VERSION     DAOSYN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59776_length_1544_cov_3.887844"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..597
                     /locus_tag="PLJ11_08135"
     CDS             61..597
                     /locus_tag="PLJ11_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOO04672.1"
                     /translation="MSLWKKVPSGRNPPEIINVIIETPKGSKNKYEVSKEFDCILLDR
                     VLHSSVVFPLAYGLIPRTFYEDGDPLDALVLISEPTFPGCVVEARPIGVLEMSDEKGQ
                     DDKILTVACGDPKNSQYLELEDIPQHYLDEISEFFKTYKKLEENKGTNVIGWQGRVRA
                     TEIVTDSLALFRNKFGYK"
     gene            complement(611..>1544)
                     /locus_tag="PLJ11_08140"
     CDS             complement(611..>1544)
                     /locus_tag="PLJ11_08140"
                     /inference="COORDINATES: protein motif:HMM:NF021416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HOO04673.1"
                     /translation="AAVIDAGLEGRIDMSERALPSETIGREAHRSDLLLLVNQGKDIG
                     SSLAFGRLVMSKVNVGQVPVVQVEEGLSIIWSGDEDALRRAGPFLDEEVLDQRGMAVP
                     AAPEGTRTISGVRPGENIWVNGHVIGRAEGTEVSLSQAPDGTLLAKGVRLKPTGIQRL
                     GGFDLRTAIVRSGQIRRTRSQPRSLPAGGKAVYLIDHCAERVLFLCRDAAYVVTVGDD
                     TSKIASSLLYRLGIPVLAITDGDEDGISDERLLYPGSHVFRLVPGTDDLVGAEIASSY
                     FEEGRGSSAPAKIEEMASIIRAACGEWLLWEERS"
BASE COUNT          298 a          505 c          464 g          277 t
ORIGIN      
        1 tgttgaacgg aaaagcataa gtaaggctag gcgatcgaag cgctcgaaga ggaaagtgct
       61 atgtcgctat ggaagaaggt cccatctgga cgcaatcccc ctgagatcat caacgtcata
      121 atcgagaccc ccaagggcag caagaacaag tacgaggtct ccaaggagtt cgactgcata
      181 ctcctggacc gcgtcctcca ttccagcgtg gtgttccccc tggcctatgg cctgatcccc
      241 cgtacgttct acgaggacgg ggaccccctc gacgcgctcg tcctgataag cgagcccacc
      301 ttccccgggt gcgtcgtgga ggcccggccc atcggggttc tggagatgag cgatgagaag
      361 gggcaggacg acaagattct aaccgtggcc tgcggagacc ccaagaacag ccagtacctc
      421 gaactcgagg acattccaca gcattatctg gacgagatat cggagttctt caagacctac
      481 aagaaactgg aggagaacaa ggggacgaac gtcatcgggt ggcagggccg ggtccgggcc
      541 acggagattg tgaccgattc cctggcattg ttccggaaca agttcgggta caagtgagcc
      601 aaggccatcc ctaggaccgt tcctcccata gcagccattc cccgcaggcc gcacggatta
      661 tggatgccat ttcctctatc tttgcgggcg cgctcgagcc tcgcccctcc tcgaaatagg
      721 agctggcgat ctccgccccc acgaggtcat cggtgccggg gaccagccgg aagacgtggg
      781 agcccgggta cagcagcctt tcatcggata ttccgtcctc gtctccgtca gtgatcgcca
      841 ggacagggat gcccaggcgg tagagcaggg aggacgctat cttgctggtg tcatccccga
      901 cggtcacaac gtaggccgcg tcccgacaga ggaagaggac gcgctcggcg caatggtcga
      961 tgagatagac ggccttcccc ccggcgggca ggctacgcgg ctgagatctc gtcctcctga
     1021 tctggccgct gcggacgatc gcggtgcgta ggtcgaaccc tcccaggcgt tggatgccgg
     1081 tgggcttgag cctgacgccc ttcgcgagca gcgttccgtc gggggcctgg gagagggaga
     1141 cctcggtccc ctccgcccga ccgatcacgt ggccgttcac ccatatgttc tcgcccggcc
     1201 gcaccccgct tatcgtcctt gtcccctcgg gggcggcggg cacggccatg cctcgttggt
     1261 ccagcacttc ttcgtcgagg aaaggccccg ccctccgcag agcgtcctcg tccccggacc
     1321 agatgatgct gagcccctcc tcgacctgga ccactggcac ctgccccacg ttcaccttgg
     1381 acatcaccaa tcgtccgaac gccagggagg agccgatgtc cttcccctgg ttcaccagaa
     1441 gcaacaggtc cgaacggtgt gcctctcggc ctatggtctc ggagggaagc gccctctcgc
     1501 tcatatcgat cctcccctcg aggcccgcgt cgatgaccgc ggcc
//
LOCUS       DAOSYN010000290         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_59826_length_1543_cov_3.045699, whole genome shotgun sequence.
ACCESSION   DAOSYN010000290 DAOSYN010000000
VERSION     DAOSYN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59826_length_1543_cov_3.045699"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1543
                     /locus_tag="PLJ11_08145"
     CDS             <1..>1543
                     /locus_tag="PLJ11_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOO04674.1"
                     /translation="AAEIIEENFQGTVANSTLIIVITSDNMTSPEARDFVLQLQKELE
                     AADLAYFEGVSSIYSYSGMVLYAAITELGPQMYEAEEQVNQSAFLLWGIPAMHAWNWA
                     GYHDSDPNETNATVYAYQSTSATLRTYLPGMDANITAIALGYYSAFADAWNATSDNTT
                     WVANPLGRAEHCVGEVAPGFITGLPLDASYQQAMLAVLYGFNITNFNSQQEVHNFTMG
                     MVSTMAGISNTTFLQQIYDLGPNYSSFQTQSRIYALAEDIVSFGTLDTYPIPIPDQLL
                     KGFVSPDNRTMLMTLSFSKDVAYITDEGETPMTDMVPDIRDIIREVGAETGFEGSTYL
                     TGAAAISKDMEDQSMDDMMLIEPITIIVILVLMGYLFRTVVGQFVPLGAVGIALGVSQ
                     AMVFVLASTIMDVNYITTTLLFALLMGVGTDYSIFIMTRYREERIKGANREQAVHTAL
                     TWAGESVATSAATVMIAFLAMTTVNFSFIRNMGIIVALAIFIALMVALTLIPSFLMLV
                     GNRIF"
BASE COUNT          314 a          490 c          441 g          298 t
ORIGIN      
        1 tcgccgcgga gatcatcgaa gagaacttcc agggcacggt ggcgaacagc accctcatca
       61 tcgtgatcac ctcggataat atgacgtccc ctgaggccag ggacttcgtg ctccagttac
      121 agaaggagct cgaggccgcc gacctagcct attttgaagg ggtcagctcc atctactcct
      181 attccgggat ggtcctctac gccgccatca ccgagctggg gccgcagatg tacgaggcgg
      241 aggagcaggt caaccagagc gccttcctcc tgtggggcat cccggcgatg catgcgtgga
      301 actgggccgg gtaccatgac tccgacccaa acgagaccaa cgccaccgtt tacgcttatc
      361 agagcacgag cgccacgctc agaacctatc tcccgggtat ggacgccaac atcaccgcca
      421 ttgcgctcgg gtactacagc gcgttcgccg atgcctggaa cgccacctcg gacaacacaa
      481 cctgggtggc caatcccctc ggccgagcgg agcactgcgt gggagaggtg gcgcccgggt
      541 tcatcaccgg cctccctctg gatgcctctt atcaacaggc catgctcgcc gtcctgtacg
      601 gcttcaacat tacgaatttc aacagccagc aggaggtgca caacttcacg atgggcatgg
      661 tgtccaccat ggccggcatc tcgaacacga ccttcctaca gcagatatat gatctggggc
      721 cgaactactc cagctttcag acgcagagca ggatctacgc cctggccgag gacatagtgt
      781 cctttgggac cctcgacacc tatcccatcc caatacccga ccagctgctc aaggggttcg
      841 tctcccctga caatcggacc atgctcatga ccctttcctt cagcaaggac gtggcctaca
      901 tcacggacga gggtgagacc ccgatgaccg acatggtccc tgacatcagg gacatcatca
      961 gggaggtcgg ggccgagacc gggttcgagg gcagcaccta tctgaccggg gcggcggcca
     1021 tctccaagga catggaggac cagtcgatgg atgacatgat gctcatcgag ccgatcacca
     1081 tcattgtgat cctggtcctg atgggctacc tgttccggac cgtcgtcggc cagttcgtgc
     1141 ccctgggggc ggtgggcatc gccctggggg tcagccaggc catggtgttc gtgctggcct
     1201 ccacgataat ggacgtgaac tacatcacca ccaccctcct attcgccctc ctgatggggg
     1261 tgggcaccga ttactccatc ttcatcatga cccggtaccg cgaggagcgc atcaagggag
     1321 cgaacaggga gcaggcggtg catacggcgc tgacatgggc aggggaatcg gtggctacga
     1381 gcgccgcgac cgtgatgatc gcgttcttgg ccatgacgac cgtgaacttc tccttcatca
     1441 ggaacatggg cataatcgtc gcccttgcca tcttcattgc tctgatggtg gcgctcacgc
     1501 tcatcccgtc cttcctgatg ctggtgggca accggatatt ctg
//
LOCUS       DAOSYN010000291         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_59864_length_1543_cov_1.920027, whole genome shotgun sequence.
ACCESSION   DAOSYN010000291 DAOSYN010000000
VERSION     DAOSYN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59864_length_1543_cov_1.920027"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..>1543
                     /locus_tag="PLJ11_08150"
     CDS             388..>1543
                     /locus_tag="PLJ11_08150"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOO04675.1"
                     /translation="MTFTESELEEAVLEWFKGLGYEYAPGPEIGPEGTRPERMDHREV
                     ILKDRLVRGLRDINPNVPDPAIDDAVAKTLTISSSSLIESNHSFHRMLVEGVDVEYKR
                     KDGTIAGDKVRLIDFEDVHNNEFLVVNQFTVHESDRARRPDVVVFVNGLPLIVMELKN
                     PTDENATIWSAFNQLQTYKRDIPSLFHFNELLVISDGLEARYGTISSDRERFMCWRTM
                     DDEREAPANMLESEVLLHGIFDKERLLDLLHHFIVFEENDRGTKTKKVAAYHQFHAAR
                     KAVNATIRAVSPGGDRRIGVVWHTQGSGKSLSMAFYAGMMIQHPVLANPTLVVITDRN
                     DLDDQLYNTFCSCHELLRQTPVQAESREHLQSLLNVASGGVVFTTIQKFFP"
BASE COUNT          398 a          394 c          402 g          349 t
ORIGIN      
        1 acatggtacg gtccgaattc tttattaaac caacgagttt gtatgttcgt acctaaagac
       61 aatgtaccta gattctatct aaagtatata ctggaaaagc caatttcctt tttcgaacga
      121 agtaaagttg gtacaacaat tatccatctc tgtaagtctg atattgacat tattagggta
      181 ttggtaccca atcatcgaat tctatcttgt tacaataccc taacaaataa tttgtacaat
      241 atcatattat ataatcataa tcagaataat attctgatgt gcatccggaa cttgctactt
      301 cctaagttaa tatctggcaa aattcgaatc gatccatcca agttcggctt cgattctgaa
      361 gaaaacaagg cggtggaggt ctaatcgttg acgttcacgg aatcggaatt ggaggaagcg
      421 gtcctagaat ggttcaaagg gctgggctac gagtacgccc ccggcccgga gatcgggccg
      481 gaggggacgc gcccggagcg catggaccat cgcgaggtca tattgaaaga ccgactggtc
      541 aggggattgc gggatatcaa tcccaacgtg cccgatcccg ccatagacga tgcggtggct
      601 aagactctga ccatatcatc gtcctcccta atcgaatcga accactcctt ccaccgcatg
      661 ttggtggaag gcgtggacgt ggagtacaag cgcaaggacg ggaccatcgc gggggacaag
      721 gtgcgcctca tcgacttcga ggatgtccac aacaacgagt tcctcgtcgt caaccaattc
      781 accgttcacg agagcgatcg ggctcggagg ccggacgtgg tcgttttcgt caacgggctt
      841 cctctgatcg taatggaact caagaaccct acggatgaga acgcgaccat ctggagcgcc
      901 ttcaatcaat tgcagacgta caagagggac atcccttcgc tctttcattt caacgagctg
      961 ctggtaatct ctgacggatt ggaagctagg tatggaacga tctcctcaga ccgagagcgt
     1021 ttcatgtgct ggaggacgat ggatgatgag cgggaagctc ccgcgaacat gctggagagc
     1081 gaagtgctgc tccacggcat cttcgacaag gaacggctgc tcgatcttct gcatcatttc
     1141 atcgtcttcg aggagaacga ccgaggaacg aagacgaaga aggtcgcggc gtaccatcaa
     1201 ttccacgcag cgcgcaaggc ggtcaacgcg accataaggg ccgtttcgcc aggcggggac
     1261 aggaggatag gcgtcgtatg gcacacgcaa ggctccggca agagcctctc catggccttc
     1321 tacgccggaa tgatgataca gcatccggta ctggccaatc cgacgctcgt cgtcatcacg
     1381 gaccgcaacg accttgacga tcagctgtac aacacattct gctcctgtca tgagctcctc
     1441 aggcagacgc cggtgcaggc tgaatcgaga gaacatctgc aatccttgct gaatgtggct
     1501 tccggagggg tggtgttcac cactatccag aagttcttcc ccg
//
LOCUS       DAOSYN010000292         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_60182_length_1536_cov_5.325456, whole genome shotgun sequence.
ACCESSION   DAOSYN010000292 DAOSYN010000000
VERSION     DAOSYN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60182_length_1536_cov_5.325456"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLJ11_08155"
     CDS             complement(<1..306)
                     /locus_tag="PLJ11_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOO04676.1"
                     /translation="MSAVLDVNVIRHDPEAVRTMLRDRKYDEDILNRFLELDREWRAV
                     VDEGNRLRKERNEASLRISKLSGEEKAEAVTRMREVAVRIGQLEAKVAELEAERDDAV
                     "
     gene            complement(308..598)
                     /locus_tag="PLJ11_08160"
     CDS             complement(308..598)
                     /locus_tag="PLJ11_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOO04677.1"
                     /translation="MPRRTLKDVKRVGHGLQPTVHVGKEGVTASLIDEVTRQVKMRKV
                     VKVRLLPSVEKDREEVAEELAERSGSVLIEVVGHIVLLCDRRYMEGRGDIDL"
     gene            complement(580..924)
                     /locus_tag="PLJ11_08165"
     CDS             complement(580..924)
                     /locus_tag="PLJ11_08165"
                     /inference="COORDINATES: protein motif:HMM:NF015965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 4"
                     /protein_id="HOO04678.1"
                     /translation="MSRKKVTNGQVRGIARKRMAILVRMSEEEALRGNLERAKRYMGL
                     ARRISVRTKVPMPEGALYCRRCSAPLVPGLNCRVRLRNHRIGTHCLECGSVRRRPYLR
                     ETKERRTCREER"
BASE COUNT          273 a          548 c          376 g          339 t
ORIGIN      
        1 cacggcgtca tcccgttcgg cctccagctc ggcgaccttc gcctccagct ggccaatgcg
       61 aacagcgacc tccctcatcc tcgtcaccgc ctcggccttt tcttccccgc tcagcttcga
      121 tatgcgcagg gaggcctcgt tccgttcctt cctgagccgg ttgccctcgt ccacgaccgc
      181 ccgccactcc ctatccagct ccaagaacct gttcaggata tcctcgtcat acttgcggtc
      241 cctgagcatc gtccggactg cctcggggtc gtgtctgatc acattgacgt ccaacacagc
      301 gctcacccta aagatcgatg tctcctctgc cttccatgta acggcggtcg catagcaata
      361 cgatgtggcc aaccacctct atcagcaccg agcctgacct ttcggccagc tcttcggcca
      421 cctcttccct gtccttctcg acgctcggca gcagcctgac cttgacgacc ttgcgcatct
      481 tgacctgcct ggtcacctcg tcgataaggg aggccgtgac cccttccttg cccacatgca
      541 cggtaggctg caggccatgc cccacccgtt tcacgtcctt cagcgttctt cgcggcatgt
      601 cctcctctcc ttggtctccc tgaggtacgg ccttcttctg acggagccac attcaagaca
      661 gtgcgtccct atcctatggt tccgcaggcg tacccggcaa ttgaggccgg gcacgagggg
      721 cgctgagcag cgccggcagt ataaggcgcc ttcgggcatg ggaaccttgg tgcggacgga
      781 gatgcgcctt gcaagtccca tatagcgctt tgcccgctcc aagttccctc tcaaagcctc
      841 ctcctccgac atccggacca ggatggccat cctcttacgg gcgatgcctc ggacctggcc
      901 gttcgtcacc ttcttcctgc tcacagggat ttccttcggg gtgccataat gccatctcgt
      961 taaataggtt tgtccgcttc ccatccaggc cgccgcccta agggaacgcc ccccgatgaa
     1021 aggccaggca gtacatggaa gggaacgatc ctttctcccc ccgacggtca atagcacccg
     1081 ccctccaatg cccaggtcat tccaatactg cgtcccgcca tcgaccgatc gccggtaccc
     1141 atcaggttat aggcgattgt gacgatagag agttgcgaca acgatcggac cgatccagct
     1201 atcatgacag gtataaggga gaaatagggc gggttaactc ctggccatat cctgagcggg
     1261 ctcgaccctc ctgaggtcgg tgaccgttct cttcgacgcc tctctccggc ggtccccgaa
     1321 aggcctaggg cccaacaccc tccgaggatc gggtacccag tcctatccat cttcccaagc
     1381 ttggaccaag gcaggctgag agtgcccaac ccacccctat cattgtaccg gatgcgttgc
     1441 atggcctggc ctaatgggag atgatcccct caatcgtccc tccccactcg gtcagggcta
     1501 tgccctccca gttgcccttt ccatcagtct tatata
//
LOCUS       DAOSYN010000293         1525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_60781_length_1525_cov_2.227211, whole genome shotgun sequence.
ACCESSION   DAOSYN010000293 DAOSYN010000000
VERSION     DAOSYN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60781_length_1525_cov_2.227211"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLJ11_08170"
     CDS             <1..316
                     /locus_tag="PLJ11_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOO04679.1"
                     /translation="GLIAGAISALSFIFLQPWLCKKAGVLDVMGVHNLHGVGGWLGAL
                     TAAIVVSGAFSANVAAAILVVVIGLGTGAICGGVIRLTRKEQEWFTDDTDFIDNPAPK
                     TQ"
     gene            649..1290
                     /locus_tag="PLJ11_08175"
     CDS             649..1290
                     /locus_tag="PLJ11_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178518.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOO04680.1"
                     /translation="MGKVEDILNDLRVSVETWDKELAIRAVDRAVEAGISPFVAIEAG
                     LARGMSTISVLFDEGRLYLPQVLAASQTMQLALERFGPLKLSESLSKGLIVLGTVQGD
                     IHEIGKNVIKAFLRGGGYTVIDLGKDVPPEEFSAAAKEHQADIVGASALMTTTLICQK
                     QVVDVLKDEGLDDVKTMFGGAPCNQGWVNSIGGDGYCACGAEVIDLVRRLLNE"
     gene            1302..>1525
                     /locus_tag="PLJ11_08180"
     CDS             1302..>1525
                     /locus_tag="PLJ11_08180"
                     /inference="COORDINATES: protein motif:HMM:NF017207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monomethylamine:corrinoid methyltransferase"
                     /protein_id="HOO04681.1"
                     /translation="MEVAERPLTVYDAYMRSTEGPKLAESHWDAKVVPEGVACMKEKY
                     DIRFGSEFIPVDRDLKERLFLAGMELLVTTG"
BASE COUNT          290 a          405 c          512 g          318 t
ORIGIN      
        1 ggggttgatc gcgggggcca tctcggcgct cagcttcatc ttcctgcagc cttggctgtg
       61 caagaaggcc ggtgtgctgg acgtcatggg cgtgcacaac ctccacggag tgggcggatg
      121 gctgggcgcg ctgaccgcgg ccatcgtcgt ctctggtgcc ttctcggcca atgtcgcggc
      181 ggccatactg gtggtcgtga tcggcctagg taccggcgca atatgcggcg gggtgatcag
      241 gctgacccgc aaggaacagg agtggttcac ggacgacacg gacttcatcg acaaccctgc
      301 accaaaaacc caatgagacc tttgtccaag gcctggcatg aatcccctca tgccggccct
      361 ttttctttct ttttagatcc ccgttcacgt agccaaaggt tattatcaat gagagttgtt
      421 aggatattcc aacatcggtt ggtccctagc ccgttcatcg catgatcgtg gccccattgg
      481 taccgcggat gtcgggtgtg gaaatggcct cctaggtggt tttagggcag aacgggcgtg
      541 caggagcgcc cgtttccccc ggacgttcct caagagggcg gtccgtccgt cccgtgggaa
      601 cgtatttgtc agccttggtg ctattcattg cgtggaggcg ggcttcccat gggtaaggtg
      661 gaggacatcc tcaacgatct ccgggtgtcg gtggagacct gggacaagga gctggccatc
      721 agagcggtgg accgtgcggt ggaagcgggc atatccccgt tcgtggccat cgaggccggc
      781 ctggcgaggg ggatgtcgac catcagtgtt ctgttcgatg aggggcgcct atacctgccc
      841 caggtgctgg ccgcatccca gaccatgcag ctggccctgg agaggttcgg tccattgaag
      901 ctcagcgaat ccctgagtaa gggcctgata gtgctgggga ccgttcaggg ggacatccat
      961 gagatcggga agaatgtgat caaggcgttc ctgaggggag ggggttatac cgtcatcgac
     1021 cttgggaagg acgtcccgcc ggaagagttc tcggctgccg ccaaggagca ccaggcggac
     1081 atcgttgggg cttccgccct catgactacc acgctcatct gccaaaagca ggtcgtcgac
     1141 gtcctgaagg acgagggact cgatgatgtc aagaccatgt tcggtggggc cccgtgcaat
     1201 cagggttggg tcaactccat cggcggagat ggctattgtg cctgcggggc cgaggtcatc
     1261 gatctggtgc gcaggttgct gaacgaatga gggagaacaa catggaggta gccgagaggc
     1321 ctttgacggt ctatgatgcc tacatgcgct caaccgaagg cccaaagttg gctgaatccc
     1381 actgggatgc caaggtggtg ccggagggcg tggcatgcat gaaggagaag tacgacatca
     1441 ggttcgggag cgagttcatt ccggtcgacc gggaccttaa ggagcgtctg ttcctagcgg
     1501 gaatggagct gctggtcacc actgg
//
LOCUS       DAOSYN010000294         1524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_60817_length_1524_cov_3.066712, whole genome shotgun sequence.
ACCESSION   DAOSYN010000294 DAOSYN010000000
VERSION     DAOSYN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60817_length_1524_cov_3.066712"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLJ11_08185"
     CDS             <1..688
                     /locus_tag="PLJ11_08185"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HOO04682.1"
                     /translation="GTGPYTWTFAEANVPPSLTITNGVDLSTDAVMAYLGCVCTDCKI
                     SCDNDGPAHATMNFVYAKETKTTSGLSSQVAETEAPFPFAYNTWDLGSSAIATTQSVE
                     ISIENNAELIYGLGSRLGTGPVFKQRAYKISLVNQFSDASVYLEKVYGASTGPAATQA
                     STEVTTAHLILDNGLSSTNQRKYTFTFSGVKFDSEAIPQSLDEIITESVDGLAKSLSV
                     AVINNTSTMP"
     gene            727..1056
                     /locus_tag="PLJ11_08190"
     CDS             727..1056
                     /locus_tag="PLJ11_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04683.1"
                     /translation="MFEKYKELDLKEFGVDEPVRIKRLSAKEQADLTNEITKLTNAKL
                     AGKSISTDLQPGMFNLLYAEKVIAGGPLPADRNKLGELDWELVAFIAEEGQRFNSPLV
                     MKVSESE"
     gene            1053..1274
                     /locus_tag="PLJ11_08195"
     CDS             1053..1274
                     /locus_tag="PLJ11_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04684.1"
                     /translation="MRFGYKHPRAVSDPRVLEAINRFQLLMLGFDSQQIDNMYVSDIQ
                     LITALYPLYYEREFERDAGAVNKGMFGGR"
     gene            1274..>1524
                     /locus_tag="PLJ11_08200"
     CDS             1274..>1524
                     /locus_tag="PLJ11_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04685.1"
                     /translation="MDGAGGDLGLKVRITPIIDQGKLSGEAKKVEKQINKTMPKDGGG
                     ILGRLGGFANKMGMGGGVGSAAIAGAVGGIIASVMNVVMG"
BASE COUNT          355 a          430 c          486 g          253 t
ORIGIN      
        1 aggcacaggg ccatatacct ggaccttcgc ggaggccaac gtccccccgt cgctcacgat
       61 aaccaacggg gtcgacctga gcacggacgc ggtcatggcc tacctgggct gtgtgtgcac
      121 cgactgcaag ataagctgcg acaacgacgg cccggctcac gcgaccatga acttcgtcta
      181 cgccaaggag accaagacca ccagcggcct cagctctcag gtggccgaga cggaagctcc
      241 cttcccgttc gcgtacaaca cctgggacct gggttccagc gccatcgcca caacgcagag
      301 cgtggagata agcatcgaga acaacgccga gctgatatac gggctcggca gcaggctggg
      361 gaccgggccg gtgttcaagc agcgcgccta caagatcagc ctggtcaatc agttctcgga
      421 cgcaagcgtg tatctggaga aggtgtacgg ggcttctacc ggaccggcgg cgacacaggc
      481 gtccactgag gtcaccacgg cccacctgat actggataat gggctgtcat ccacgaacca
      541 gcggaagtac accttcacct tcagcggtgt caagttcgac agcgaggcca tcccgcaatc
      601 gttggacgag ataataacgg agagcgtgga cggcctggcc aagtcgctca gcgttgcggt
      661 catcaacaac acctcgacaa tgccgtaaac cccttcccaa tctcttagcg atgcaacgga
      721 gtatgtatgt tcgagaagta caaggaactg gaccttaagg agttcggcgt agatgagccg
      781 gtcaggatca agcggctgtc cgccaaggag caggccgacc tgaccaatga gataacgaag
      841 ttgacgaacg ctaagctggc cggtaagtcc atatccaccg acctacagcc gggaatgttc
      901 aatctgctgt acgcggagaa ggtgatcgcg ggcgggccgc taccggcgga ccgaaataag
      961 ctgggcgaac tggactggga gctggtcgcg ttcatagcgg aggaggggca gaggttcaac
     1021 agccctttag tgatgaaggt ttccgagagc gagtgaggtt cgggtacaag catccgaggg
     1081 ccgtgagcga cccccgtgta ttggaggcca tcaaccgctt ccagctcttg atgcttggct
     1141 tcgattcgca gcagatcgac aatatgtacg tctctgacat ccaactgata accgctctat
     1201 atccactata ctatgagagg gagttcgagc gggacgccgg ggccgtgaac aagggcatgt
     1261 tcggggggcg gtgatggacg gcgcgggcgg cgaccttggc ctaaaggtcc gcataacccc
     1321 catcatcgac cagggcaagc tcagcgggga agcgaagaag gtcgagaagc agatcaacaa
     1381 gaccatgccc aaggacggcg ggggcatcct cggaaggctg gggggcttcg ccaacaagat
     1441 gggcatggga ggcggcgtcg gaagcgcggc catagcgggg gccgtgggcg gcatcatcgc
     1501 ttccgtgatg aacgtcgtca tggg
//
LOCUS       DAOSYN010000295         1517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_61193_length_1517_cov_3.640219, whole genome shotgun sequence.
ACCESSION   DAOSYN010000295 DAOSYN010000000
VERSION     DAOSYN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61193_length_1517_cov_3.640219"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1467)
                     /locus_tag="PLJ11_08205"
     CDS             complement(244..1467)
                     /locus_tag="PLJ11_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOO04686.1"
                     /translation="MDCRSPVMMNDAEIEAAKEHFGKVLREQLERVERMKASPGWIDH
                     SKLDRLIIGVVGGDGIGPYICRHAEGVLRELLKEEIAAGKVEVRPIDGLTIENRARQL
                     KAIPDEVLAELRKCHVILKGPTTTPKKGDGWPNIESANVAMRRELDLFANVRPVRIPE
                     QGIDWTFFRENTEGAYVLGSRGVDVNEDIAIDFTVATTQGCERIIRLAFEHARRTGVN
                     RVTVVTKANVIKRTDGKFLSVAERIAKDYPEVKWDDWFIDIMTAKLLDPYRRAEFKVM
                     VLPNLYGDIITDEAAQIQGGVGTAGSANIGARYAMFEAIHGSAPRMVDEGRAQYADPS
                     SIIKAAAMLLNHVGFVEKARRLEMALDICTQFERRVRVTGRADGATGEEFGRYVLETV
                     AREDLETEWKKASGC"
BASE COUNT          244 a          528 c          393 g          352 t
ORIGIN      
        1 atgggagata ctgatattcg gatcggttcc atttcaacac catatcggaa cgacctctgt
       61 gttacgcttt caaaatccat ccccgcttat cgtattggct tctgtccccg tttctatcat
      121 gggcggtcac caagtaaata agttgataaa aatttcttcc ttagcccttc cctgggagct
      181 cgatcgctcc gaggatgctt tcattatccc ctcgtttcaa tggaatgcta cctgaaaggt
      241 tttttagcag ccgctggcct tcttccattc cgtttccagg tcctcccttg ccacggtctc
      301 cagcacgtac ctcccgaact cctcgcccgt ggcgccgtcg gccctccccg tcaccctcac
      361 cctccgctcg aactgcgtgc atatgtccag ggccatctcc agccttctgg ccttctccac
      421 gaagccgaca tggttcagca gcatcgccgc cgccttgatg atggagctcg gatcggcgta
      481 ctgcgccctt ccctcgtcca ccatgcgcgg ggcggagccg tggatggcct cgaacatcgc
      541 gtagcgggcg cctatgttcg ccgagccggc cgtacccacc cctccctgga tctgcgccgc
      601 ctcgtccgtg atgatatccc catataggtt aggaagcacc atcaccttga actccgccct
      661 gcggtaggga tctaggagct tggcggtcat gatgtcgatg aaccagtcgt cccacttgac
      721 ctccgggtag tccttggcta tgcgctccgc aacgctcagg aacttgccgt ccgtcctttt
      781 tatcacgttc gccttcgtga ccacggtgac gcggttgacg cccgtccttc gggcatgctc
      841 gaacgccagc cggatgatgc gctcacagcc ttgggtggtg gcgacggtga agtcgatggc
      901 gatgtcctcg ttcacgtcca cgcccctgct cccgagcacg tacgccccct cggtgttctc
      961 cctgaagaac gtccagtcga tgccctgctc cggaatgcgc accggccgca cgttcgcgaa
     1021 gaggtccagc tcccgccgca tggcgacgtt ggcggactcg atgttcggcc agccgtcccc
     1081 cttcttgggg gtggtggtcg gccccttgag gatgacatgg catttcctca gctccgccag
     1141 cacctcgtcc gggatggcct tgagctgccg ggcgcggttc tctattgtaa gcccgtcgat
     1201 cggccgcacc tcgaccttcc cggccgcgat ctcctccttg agcagctccc gcagcacgcc
     1261 ctccgcatgc ctgcagatgt acggcccgat gccgtccccg cccaccacgc cgatgatgag
     1321 cctgtccagc ttggagtggt cgatccagcc gggggaggcc ttcatacgct ccaccctctc
     1381 cagctgttcc ctcaaaacct ttccgaaatg ctccttggcg gcctctatct cggcatcgtt
     1441 catcatgacc ggggaacggc agtccatcac cgcttattac gtttttgcat aaggggccgt
     1501 cgcgcgcatc aagaaat
//
LOCUS       DAOSYN010000296         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_61352_length_1514_cov_2.708019, whole genome shotgun sequence.
ACCESSION   DAOSYN010000296 DAOSYN010000000
VERSION     DAOSYN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61352_length_1514_cov_2.708019"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..>1514)
                     /locus_tag="PLJ11_08210"
     CDS             complement(60..>1514)
                     /locus_tag="PLJ11_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04687.1"
                     /translation="LMRRVEDLAKLRTEGGKGAIVVSTQVIEAGVDLSAGKMVTELAP
                     WSSLVQRFGRCNRYGEHPVGEIKVVLPPEGKMERFAEPYSPEQLEHSLELLAAMPGDF
                     DSATLSKEFDHPEQLDVLRRTDLLELFDTTADLFGKFTDVSRFVRAAEDVSVQVFWRD
                     LKESGPATQPMPLKRELCPAPLYEVRDMMKRAGAPRFFTIDPTLGEWVPARANEVVPG
                     MVLMLDAAEGMYTSSLGWDKGSKARVTPLLDRAAEQEVEPPDSYSWDRPGREERTIEE
                     HSMDVLGVARRVITNLPIPERTKAEALEAALWHDAGKAHAVFQHMLGNGEGKVLLAKA
                     RGMLTGKAREEAIREGKVRPFFRHELVSGLMCLELGKGFLTSYLTAAHHGKVRVSIRS
                     VPTERGPPDGERFARGVWENDEVPIPLEVPEGKPRTFRVDLSVMEMGERDGRPSWTRQ
                     VLELRGSPEMGPFRLAMLEGLIKSADERASGGER"
BASE COUNT          239 a          552 c          432 g          291 t
ORIGIN      
        1 gagcccgcgc aggtaggagg cccacggctc ggtgcggcag ttgctcagct cgaccctcat
       61 catcgctccc ccccgctcgc cctctcgtcc gcggatttga tcagcccttc cagcatcgcc
      121 aaccggaagg ggcccatctc cggcgagccc cggagctcca ggacctgcct tgtccagctt
      181 gggcggccgt cccgctcccc catctccatg acggagaggt cgaccctgaa cgtccgcggc
      241 ttcccttcag ggacctccaa cggaatcggc acctcgtcgt tctcccagac ccctcgggcg
      301 aaccgttccc catcaggcgg tcccctttcc gttgggacgg acctgatgga taccctcacc
      361 ttgccgtgat gggcggcggt gagatagctt gttaggaagc ccttgccgag ctccaggcac
      421 atgagccccg acacgagctc gtgccggaag aaggggcgga ccttcccctc gcgtatcgcc
      481 tcctcccgcg ccttcccagt gagcatgccc ctcgccttgg cgaggagcac cttcccttcc
      541 ccgttcccga gcatgtgctg gaagaccgcg tgcgccttac cagcatcatg ccagagggcc
      601 gcctccagag cttcggcctt cgttctctct ggtattggaa ggttggtgat gacccgtctc
      661 gcgacgccta gcacgtccat cgaatgctcc tctatggtgc gctcttccct tccaggtcgg
      721 tcccaggaat atgagtcggg aggttccacc tcctgctccg cggcccggtc gaggagcgga
      781 gtgaccctgg ccttcgaacc cttgtcccat cccagagagc tggtgtacat tccctcggcc
      841 gcatcgagca tgagcaccat ccccggaacg acctcattgg cacgggcggg aacccattcc
      901 cccagggtcg gatcgatggt gaagaatcgg ggagcccccg ctctcttcat catgtccctg
      961 acctcgtaga ggggagcggg gcacagctcc cttttcaagg gcatgggctg tgtggccggt
     1021 ccgctttcct tgaggtccct ccagaacacc tggacgctca catcctccgc ggcccgaacg
     1081 aaccgggaga cgtcggtgaa cttgccaaag aggtccgccg tggtgtcgaa gagctcgagc
     1141 aggtccgtcc tgcgcaggac gtccagctgc tcggggtgat cgaactcctt ggagagcgtc
     1201 gccgagtcga aatcccctgg catcgcggcg aggagttcca ggctatgctc cagctgctct
     1261 ggggaatacg gctctgcgaa ccgctccatc ttcccctctg ggggcaggac caccttgatc
     1321 tcccccaccg gatgctcccc gtacctgttg caccggccga acctttgcac cagcgaggac
     1381 cacggcgcaa gctccgtgac catttttccg gccgagaggt ccacccccgc ctcgatcacc
     1441 tgcgtggata cgacgatggc gcccttgccc ccctcggtcc tgagctttgc caggtcctcc
     1501 acccgcctca tcag
//
LOCUS       DAOSYN010000297         1511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_61508_length_1511_cov_4.224588, whole genome shotgun sequence.
ACCESSION   DAOSYN010000297 DAOSYN010000000
VERSION     DAOSYN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61508_length_1511_cov_4.224588"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLJ11_08215"
     CDS             complement(<1..661)
                     /locus_tag="PLJ11_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOO04688.1"
                     /translation="MAKVSAKAKAAAKPAGRKVAPKRASKNIVVECMTAVPLASQEVE
                     LVERKGIGHPDSISDGIAEAVSLALCKMYLERFGEILHHNTDQNEVVGGQSTPRFGGG
                     EVLEPIHIMLSGRAITQVNGERLPYSTTAINATKEYLKRFPNLDVYRHVTVDCRIGKG
                     SLDLIKNFEALKDKCKMDGHGNHCFLANDTSFGCSYAPYSDTELVCLETERYVNGAMK
                     KR"
     gene            783..1346
                     /locus_tag="PLJ11_08220"
     CDS             783..1346
                     /locus_tag="PLJ11_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO04689.1"
                     /translation="MRFPSESEIKRLRKSLDITQLELARLSGVSQSTIAKLERGGIKG
                     SYETVTKIFIALQEVARKRSKASKAGDLATRRIVGIQANEPVKAATELMRSSGISQMP
                     VFQGRQHVGSISENRILERLREGERMEAIGERRVSDLMEDAFPIINEDTPIEAVTALL
                     SSSHAVMVGRNGNMTGIITSADLLKLL"
BASE COUNT          299 a          442 c          456 g          314 t
ORIGIN      
        1 gccgcttctt catggccccg ttcacgtatc tttccgtctc caggcacacc agctcggtgt
       61 ccgagtaggg ggcgtaggag cagccgaagg acgtgtcgtt ggccaggaag cagtggttgc
      121 cgtggccgtc catcttgcac ttgtccttca gggcctcgaa gttcttgatc aggtccaagg
      181 accccttgcc gatgcggcag tccaccgtga catgccggta cacgtccagg ttggggaagc
      241 gcttcaaata ttctttggta gcatttatgg cggtggtgct gtacggcagc ctctcgccat
      301 tgacttgggt gatcgccctc cccgagagca ttatatgtat aggttctaaa acctccccgc
      361 ccccgaacct gggggtgctc tgccctccca ccacctcgtt ctggtcggtg ttgtgatgga
      421 ggatctcccc gaacctttcc agatacatct tgcagagggc gagggagacc gcctctgcga
      481 tgccgtccga gatgctgtcc ggatggccga tccccttcct ttcgaccagc tcgacctcct
      541 gacttgccaa cgggacggcg gtcatgcatt caaccacgat gttcttggat gcccgcttgg
      601 gggcgacctt gcggcccgcc ggcttggcgg cggccttcgc cttcgcgctt accttagcca
      661 tgagatgctc accttattgc gaacggaccc cttccgggcc ctggagggtt cttggaattt
      721 cctgttctga tataagggtg attgcctcaa atattcatat atgaataatc atatcccgtg
      781 tcatgaggtt cccttcagag agcgagatca agcgcctccg caagtccctg gacatcaccc
      841 agctcgagct ggcgaggctc agcggggtga gccaatccac cattgccaag ctggaaaggg
      901 ggggtataaa gggcagttac gagacagtca cgaagatctt catcgccctg caggaggtgg
      961 cgcgcaagcg ttccaaggcc agcaaggccg gggacctcgc cacccgaagg atagtgggca
     1021 tccaggccaa tgagccggtc aaggcggcca cggagctgat gcgctcctct ggcatatcgc
     1081 agatgcctgt gttccaggga cgccagcatg tgggcagcat aagcgagaac cggatcctgg
     1141 aaaggctgag ggagggcgaa cggatggagg cgatcgggga acggagggtc agcgacctca
     1201 tggaggacgc ctttcccatc atcaacgagg acaccccgat cgaggcggtg accgcgctcc
     1261 tgagcagctc gcacgcggtc atggtcggca ggaatgggaa catgaccggg ataatcacct
     1321 cggccgacct gctcaagctc ctctgaagga tgctcctgaa gattattctt agatgaatta
     1381 caacgctcgc cggaccacga aggaatccag gtcgttcggt atgatcgtgc tggggaatat
     1441 tcccctggcc tcctccagaa ggggggaggg gtcctcatac ctcccgctga tgtgcaccag
     1501 atagagcgag g
//
LOCUS       DAOSYN010000298         1508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_61700_length_1508_cov_2.742602, whole genome shotgun sequence.
ACCESSION   DAOSYN010000298 DAOSYN010000000
VERSION     DAOSYN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61700_length_1508_cov_2.742602"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLJ11_08225"
     CDS             <1..369
                     /locus_tag="PLJ11_08225"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOO04690.1"
                     /translation="RSDRGTKALPRALVECGWAVTEVPVYSPLLSQSEDMELLLDRME
                     RREVAALAFPTPLQTEAFFVQFFERFDREEGMALLDGVAIAAMSAEVVKRLDDHGVQA
                     DIIPKMATAELLIEAVADHL"
     gene            complement(360..1163)
                     /locus_tag="PLJ11_08230"
     CDS             complement(360..1163)
                     /locus_tag="PLJ11_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO04691.1"
                     /translation="MITLRNVSFEYPGGHRALEDVTLEVGEGERVALIGPNGAGKSTL
                     LHIIAALEFPRVGEVRVMGQRVDRRSAAEARRKVGFLFQDPDDQVFMPSVYEDVAFGP
                     LNMGLPEEEVRRRVEGALEASGAAHLARRVPQNLSLGEKKRVAIAGLLSMAPPLLLLD
                     EPTANLDPQGRRDLVNVLAGLGQSMVIATHDMSVAFELAERAVVLKGRVLFDGDVRTL
                     LKRPEVLREANLELPSFSRLMMMYREHIGADFEPPLTVEEALALLKAHR"
     gene            complement(1160..>1508)
                     /locus_tag="PLJ11_08235"
     CDS             complement(1160..>1508)
                     /locus_tag="PLJ11_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO04692.1"
                     /translation="LLTDMIMLTYRFIFVLLEELERMRLARKARGFQGGRSLLDRKAF
                     QVLANTIGMVFLRSYRRAGQVYAALLSRGYDGKARTIATYRVGGRDLALGIVFVITGV
                     WTLALQMGWYVWP"
BASE COUNT          256 a          526 c          482 g          244 t
ORIGIN      
        1 cgctccgaca ggggcaccaa ggcactcccc cgggccctgg tcgaatgcgg atgggccgtg
       61 accgaggtcc ccgtctactc cccccttctt tcccagtccg aggacatgga gctgctgctc
      121 gaccgcatgg agaggcggga ggtggccgcc ctggccttcc ccaccccgct ccagaccgag
      181 gcgttcttcg tgcagttctt cgaaaggttc gatagggagg agggcatggc cctgctggac
      241 ggcgtggcca tagcggccat gagcgccgag gtggtcaaga ggctggacga tcatggcgtc
      301 caggcggaca tcatcccgaa gatggccacg gcagaactcc tgatcgaggc cgtggcggat
      361 cacctgtgag ccttgaggag cgccagcgcc tcctcgacag tgagcggggg ctcgaagtcc
      421 gccccgatgt gctccctgta catcatcatc aggcgggaga aggagggcag ttcgagattg
      481 gcctccctga gcacctcagg ccgtttgagc agcgtacgga cgtcgccgtc gaagagcacc
      541 ctgcccttca gcaccaccgc ccgctccgcc agctcgaacg ccaccgacat gtcgtgggtg
      601 gcgatgacca tgctctgccc cagccccgca aggacgttga ccaggtccct cctcccctgg
      661 gggtcgaggt tggcggtcgg ctcgtccagg agaagcaggg gaggggccat ggagagcagg
      721 ccggcgatgg ccacccgctt cttctccccc agggaaaggt tctgcgggac cctccgtgcg
      781 aggtgggcgg ccccgctggc ctccaatgcg ccctccaccc tcctccggac ctcctcctcc
      841 ggcagcccca tgttgagggg gccgaaggcc acgtcctcgt agacgctggg catgaacacc
      901 tggtcatcgg ggtcctgaaa gaggaagccg accttgcggc gcgcctccgc ggcggaccgc
      961 ctgtccaccc gttggcccat gacccgcacc tcccctacgc gtgggaactc gagggccgct
     1021 atgatgtgga gcagcgtgct cttccccgcc ccgttcggac cgatgagggc cacgcgctcc
     1081 ccttccccca cctccagggt gacatcctcc agggcgcggt gccctcccgg gtattcgaag
     1141 gagacgttcc tgagcgtgat cacggccaaa cataccaccc catctgcagc gccaatgtcc
     1201 atacgcctgt tataacgaac acgatgccca gggccaagtc cctgcccccg acccggtagg
     1261 tggcaatggt cctggccttt ccatcgtacc cccgggagag cagggcggcg tacacctggc
     1321 cggcccggcg ataggagcgc aggaagacca tgcctatggt gttcgccagg acctggaagg
     1381 ccttccggtc caggagggag cggccgccct ggaagcccct ggccttgcgg gccaggcgca
     1441 tcctctccaa ttcctccagc agcacgaaga tgaacctgta ggtgagcatg atcatgtcgg
     1501 tgagcagg
//
LOCUS       DAOSYN010000299         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_61945_length_1504_cov_1.876467, whole genome shotgun sequence.
ACCESSION   DAOSYN010000299 DAOSYN010000000
VERSION     DAOSYN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61945_length_1504_cov_1.876467"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1090)
                     /locus_tag="PLJ11_08240"
     CDS             complement(29..1090)
                     /locus_tag="PLJ11_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04693.1"
                     /translation="MDPDTPHDKVATKEIAPGISVILYIKDDKSEAQAYRFDKDKFTA
                     AQAKKWLKDNGIKCKGFEPAEGTRDESKKQSNGRPRYLNININDRMFERSDKGMLIHD
                     AILLAEGTWTDSYQQTPCKYKSEVLEKYSNNWLDNSIWSRHPGGTPRSVDEKIGQVVN
                     PHYDPKYKAVMGDISFHMMSQKSRDIAEMIDAGLVNAVSVELGGTEKYNAKDKCYEAE
                     TIDFFGLAVVDKGACETCLIRHKSNGDLVILPDAGQDGMPPQEGESMDIEELAKAVQD
                     LAARVAAIEDKLTPKEEAPEGPAAEEKEMSKKLDDAIRKLSEAEERIRKLENDPKPRA
                     YAGGGNAAKYTKTLISEEI"
     gene            complement(1126..>1504)
                     /locus_tag="PLJ11_08245"
     CDS             complement(1126..>1504)
                     /locus_tag="PLJ11_08245"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HOO04694.1"
                     /translation="RNLAALKGITDDMSKKISSELSEGILKGEDMRRLSKRITESTNI
                     PIERARVMARTETMYAFNIAANEEYKAYGVGKVEWVAALDERMCDICGKYHGKIYPID
                     EAPDAPAHPNCRCVKIPYIEEVA"
BASE COUNT          276 a          437 c          340 g          451 t
ORIGIN      
        1 agttatgtca gccatgtcaa tctctccttc agatctcctc gctgatcaaa gtcttcgtgt
       61 acttcgcagc gttccctccg cccgcatacg ctcggggctt ggggtcgttc tcgagctttc
      121 tgatgcgctc ctcggcttcc gacaactttc ggatggcatc atccagcttc ttgctcattt
      181 ccttctcctc tgccgcgggc ccctcgggag cctcctcttt gggagtcagc ttatcctcga
      241 tggcagccac tctagcggcg aggtcctgca ctgctttcgc aagttcctct atgtccatac
      301 tttctccctc ctggggaggc attccatctt ggcccgcatc gggcaaaata accaagtcac
      361 cattagattt atgccggatg aggcatgttt cgcaagcgcc cttatccacc acggccaggc
      421 cgaagaagtc gatcgtctcg gcctcatagc atttatcctt ggcattgtat ttttcggtcc
      481 cgcccaattc gacggatacc gcattgacaa gccctgcatc tatcatctcc gcgatgtccc
      541 gggacttctg cgacatcata tggaatgata tgtcgcccat tacggccttg tatttcggat
      601 catagtgagg gttgacaact tgcccgatct tctcatccac cgatcgcgga gtgcccccgg
      661 gatgcctgga ccagatgctg ttatcaagcc aattattgct gtatttctcc aatacttcgg
      721 acttatattt gcatggtgtc tgctgatagg aatccgtcca ggtcccctcg gccaagagta
      781 tcgcatcatg tatgagcatt cccttatcgc tgcgctcgaa catgcggtca ttgatattga
      841 tattgagata gcgcggcctt ccattgctct gcttcttcga ttcgtcccga gtcccttcgg
      901 ctggctcgaa ccctttgcat tttatgccat tgtccttgag ccacttcttg gcctgagctg
      961 ccgtgaattt gtccttgtcg aacctatagg cctgcgcttc ggacttgtcg tccttgatgt
     1021 ataggatgac ggatattcca ggcgcgatct ctttcgtcgc caccttgtca tgaggcgtgt
     1081 ccgggtccat cactctgcat gcatgctgat taggatatgg cattattatg caacctcctc
     1141 aatatatggg attttaacgc atcggcaatt aggatgagca ggggcatctg gcgcttcatc
     1201 gatcggatat atttttccgt gatattttcc gcagatatcg cacattcttt catctagcgc
     1261 ggccacccat tcgaccttcc ctactccata tgctttatat tcctcattgg ccgcgatatt
     1321 gaaagcgtac atcgtttcgg tcctggccat tacccgcgcc ctttctatcg ggatattggt
     1381 cgattcagtt atgcgcttgg acaaccggcg catatcctcg cctttaagga ttccctcgga
     1441 tagttcggat gatatcttct tcgacatatc atctgttatg cccttgagcg cagccaggtt
     1501 ccgt
//
LOCUS       DAOSYN010000300         1495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_62434_length_1495_cov_3.866667, whole genome shotgun sequence.
ACCESSION   DAOSYN010000300 DAOSYN010000000
VERSION     DAOSYN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62434_length_1495_cov_3.866667"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1495)
                     /locus_tag="PLJ11_08250"
     CDS             complement(<1..>1495)
                     /locus_tag="PLJ11_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04695.1"
                     /translation="TKIAEAAALLAKVKGMDAAAAETERLLTLADQSYKEKEYKEALA
                     YATKSVEASVRARRERLQGMLREASALLERVAPLGRADEGTLRAIARAAEAINAGDLD
                     RALELSREAWDAAERAANRVLSDAFGRVQSSLLFAEGLELKVEKQRAALRGCREALEA
                     GNVAKTVDLILSLTEALRALALGRFAERAGRAEAAFALGDRFPMELAEAKERLAKGRE
                     LMTSGSVEEAFAALDAAEGAFSSAFSKAVGRRARELRERAERVSWLRKVDLGDLEGRA
                     RRLELEESYPEAVAVLEEGREKVRAVEREVLLRRLAALQPRLRLAHMTRRDVSAALRR
                     MEEAREALRRDEPEAAFALVEEASALVEEGLQGYAEVEAELLSVQELRSRCDGLGLAC
                     AEGDKLMTSAKRLVLRGEFAQAAEALRGAQRAHRLSLETHFATGIMRLEMQLATAIRL
                     GAEVAAESAALEALTAKVRSGDFELVHGSLVTIADTVEERTRAAVGE"
BASE COUNT          173 a          652 c          444 g          226 t
ORIGIN      
        1 tgctcgccca ccgccgcccg cgtccgctcc tccaccgtgt cggcgatggt caccagggag
       61 ccgtggacga gctcgaagtc cccgctcctg acctttgccg tcagcgcctc cagggcggcg
      121 ctctccgccg ccacctccgc ccccagccgg atcgcggtgg ccagctgcat ctccagccgc
      181 atgatgccgg tggcgaagtg cgtctccagg gagaggcggt gcgcgcgctg cgccccccgc
      241 agcgcctccg ccgcctgggc gaactccccg cgaaggacga gacgcttcgc ggacgtcata
      301 agcttgtccc cctcggcgca ggcgaggccg aggccgtcgc accgcgaacg gagctcctgg
      361 acgctcagga gctccgcctc cacctcggcg tagccctgca gcccttcctc caccagggcg
      421 gacgcctcct ccaccagcgc gaacgcggcc tcgggctcgt ccctccgcag ggcctcccgc
      481 gcctcctcca tgcgccgcag cgccgcggag acgtcccggc gcgtcatgtg cgccagcctg
      541 agccggggct gcagggctgc caggcgccgc aggaggacct cccgctccac cgcccggacc
      601 ttctcccgcc cctcctccag caccgcgacc gcctcgggat agctttcctc cagctccagc
      661 cgcctcgccc tcccctcgag gtcgcccagg tccaccttcc ggagccagga tacccgctcg
      721 gcccgctccc tcagctccct cgcccttctt ccaacggcct tgctgaacgc gctggagaac
      781 gcgccctccg ccgcgtccag ggcggcgaac gcctcctcca cgcttcccga ggtcatgagt
      841 tccctcccct tggcgaggcg ctccttcgcc tccgcgagct ccatcgggaa cctgtccccg
      901 agggcgaacg ccgcctccgc cctccccgcc cgctccgcga acctgcccag ggcaagcgcc
      961 ctcagcgcct cggtcaggga gaggatgagg tcgaccgtct tggccacgtt ccccgcctcg
     1021 agggcctcgc ggcacccgcg cagggctgcc cgctgctttt ccaccttgag ctccagcccc
     1081 tcggcgaaga gcagggagga ctgcacccgc ccgaaggcgt ccgagagcac gcggttcgcg
     1141 gcgcgctccg ccgcgtccca ggcctccctg ctcagctcga gcgcgcggtc gaggtccccc
     1201 gcattgatgg cctccgccgc cctcgcgatc gccctgagcg tcccctcgtc cgccctcccc
     1261 agcggggcca cgcgctccag gagcgcggac gcctcgcgca gcatcccctg cagccgctcc
     1321 ctccgggccc ggacggacgc ctccacgctc ttcgtggcgt acgccagcgc ctccttgtac
     1381 tccttttcct tatagctctg gtccgccagg gtcaggagcc tctccgtctc cgcggcggcg
     1441 gcgtccatcc ccttgacctt ggccaggagg gcggccgcct ccgctatctt cgttt
//
LOCUS       DAOSYN010000301         1490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_62709_length_1490_cov_4.101742, whole genome shotgun sequence.
ACCESSION   DAOSYN010000301 DAOSYN010000000
VERSION     DAOSYN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62709_length_1490_cov_4.101742"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PLJ11_08255"
     CDS             <1..565
                     /locus_tag="PLJ11_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04696.1"
                     /translation="GGAPTGSVSSSAPALPPQERTPRADVKGEKGALPRRELLIALVA
                     MLTMAPLSPWVTTFGGLIGCCALESSALSGRYIALLGIGGLAVLFSERLVPALAMRRA
                     ALLGVGAGAVGLTAMDIVDIIGYNADAFSSLEMAAPGLGMYIAIALGAGVIVAVLVPA
                     RHGSPSSRRAHAPVRRGSPGSALSARR"
     gene            complement(522..>1490)
                     /locus_tag="PLJ11_08260"
     CDS             complement(522..>1490)
                     /locus_tag="PLJ11_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOO04697.1"
                     /translation="GVPMVALRSARVMRKLLLGADQRTLAQIGEVELAKMKRAQAYIG
                     IRGSFNITENSDVPAEKMDLYMKHWSGPVHTDWRVPNTKWVVLRWPTASMAQQAQMST
                     EGFEDFYFNTCTLDYSKMSRAMDPLVELMERTDRVRIVGPGTDLGFSIKGIPVVKCDG
                     ERNIPDGEVYTAPVKGSVNGTISYNTKTLYQGKVFENVVLRFKDGRIVEATSSNTKDM
                     NAILDTDEGARYIGEFAIGVNPFINKAMLDILFDEKIAGSIHFTPGNAYKEADNGNRS
                     TVHWDLVLCQTPEWGGGEIYFDGRLVRKDGRFVIDELKGLNPESLV"
BASE COUNT          221 a          488 c          481 g          300 t
ORIGIN      
        1 gggcggggct ccgacgggat cggtgtcatc gagcgcgccc gcccttccgc cccaggaaag
       61 gacgccaagg gcggacgtca agggcgagaa gggggcgctg ccccggaggg agctgctgat
      121 agcactggtg gcgatgctca ccatggcgcc gctcagtccg tgggtcacca ccttcggggg
      181 cttgatcggg tgctgcgccc tcgagagcag tgccctgtcc ggaaggtaca tcgccctcct
      241 gggcatcggc ggcctggccg tgctcttctc cgagcgcctg gtgccggccc ttgcgatgag
      301 aagggccgcc ctgctcggcg tcggggcggg ggcggtggga ttgaccgcga tggacatcgt
      361 ggacataatt gggtacaatg ccgatgcctt ctcctcccta gaaatggccg cacctggtct
      421 gggcatgtac atcgccatcg cgctcggggc gggcgtgatc gtcgccgtcc tggtccctgc
      481 aaggcatggc agcccctcct cccggcgcgc gcatgcgccc gtcagacgag gctctccggg
      541 ttcagccctt tcagctcgtc gatgacgaag cggccgtcct tgcgcaccag ccttccgtcg
      601 aagtagatct ccccgccgcc ccattccggg gtctggcaca gcacgaggtc ccaatggacg
      661 gtggacctgt tgccgttgtc ggcctccttg tacgcgttcc cgggggtgaa atggatggag
      721 ccggcgatct tctcgtcgaa gaggatgtcc agcatggcct tgttgatgaa cgggttgacg
      781 ccgatggcga actccccgat gtacctagcc ccctcatccg tgtccaggat cgcgttcatg
      841 tccttggtgt tggagctggt ggcctcgact atccttccat ccttgaacct caggaccaca
      901 ttctcgaaca ccttcccctg gtacagggtc ttggtgttgt agcttatggt cccgttgacg
      961 gagcccttca cgggggcggt gtagacctcc ccgtccggaa tgttccgctc cccgtcgcac
     1021 ttgaccaccg ggatcccctt gatcgagaag cccaggtccg tgccggggcc gacgatgcgc
     1081 accctgtccg tgcgttccat gagttccacc agggggtcca ttgcccggga catcttggag
     1141 tagtcgagcg tgcaggtgtt gaaatagaag tcctcgaagc cctcggtgga catctgggcc
     1201 tgctgggcca tggaggcggt gggccagcgc agcaccaccc acttcgtgtt cggcaccctc
     1261 cagtcggtat gcacaggccc ggaccagtgc ttcatgtaga ggtccatctt ttccgccggg
     1321 acgtcggagt tctcggtgat gttgaacgat ccccggatcc cgatgtacgc ctgggccctc
     1381 ttcatcttgg ccagctcgac ctcgcctatc tgggcgaggg tcctctggtc ggcgccaagg
     1441 agcagcttcc gcatcaccct ggcggacctg agggcgacca tcggcacccc
//
LOCUS       DAOSYN010000302         1484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_63076_length_1484_cov_3.380686, whole genome shotgun sequence.
ACCESSION   DAOSYN010000302 DAOSYN010000000
VERSION     DAOSYN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63076_length_1484_cov_3.380686"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..943)
                     /locus_tag="PLJ11_08265"
     CDS             complement(422..943)
                     /locus_tag="PLJ11_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HOO04698.1"
                     /translation="MATAPSSGSGPRPRWKARSWGATAPEGEGGHGPGPTRKLSAVHR
                     PGRSRPPQGRGAGGAGLPGGDVHLLRFGPERSTCPGGTAPPPGEAHWHVDHLLAQGQP
                     VGALLVYSEARLECRLAEELSRLEGVRRHVPGFGASDCRCPGHLLLCEEPVQAVGGAI
                     QGVLNRLQDGPLG"
     gene            complement(867..>1484)
                     /locus_tag="PLJ11_08270"
     CDS             complement(867..>1484)
                     /locus_tag="PLJ11_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase family protein"
                     /protein_id="HOO04699.1"
                     /translation="MGLARGRGRKTCQISAAEKAIIEEYDAAIFMLGNFKECVTHEKI
                     NLYKDIQVPVVVVIGPELDSLPDCEALVCGVGRKVERMRRSEEIAKLEEIADRVKEVV
                     DRRRKEIEEDPLFVHPSEVKDALEDLDAVQECLRPSPVVLHVDGLRVKIPYKDHVREL
                     EEVEVYGHKLKDIATISDCRLGDSTIIRIRTKAEVEGKVLGCDRP"
BASE COUNT          249 a          533 c          397 g          305 t
ORIGIN      
        1 cgaaaccgcc tttcagtctc ctcaagctca tcccgatact cggccatcaa agcgttcagt
       61 tccttgatct tcctttccag cttggtgcgc ttgttaggaa acatcgtacg aataaataat
      121 taaatgagcg aataaaaggt tttgggacca catccactat atggacggca atgctctcct
      181 tggcccgtat gcgaccccct tggcctacca gtgagaccag gcggtctaga gcgctccggc
      241 cgcctatcgc ccttgcccct ttccaaatgc ggtcccccca ttcaccttga gggggccgtc
      301 cgacctggga aagggatgtc catctataac aggctctcct gcccggttcc ccgtctcaat
      361 gccattggcg cgcctccgcc ctaaaggcaa gcgtcccatc ctcgtcgcct tggccctgcc
      421 tttaccctag gggaccgtcc tgcagtctat tgagcacgcc ctggatcgcc cctcccaccg
      481 cctggacggg ttcctcgcac agcagcagat gcccggggca gcggcagtcc gaggcgccga
      541 agccgggaac gtgcctcctc actccctcca agcgggagag ctcctcggcg aggcggcatt
      601 ccagcctcgc ctccgagtac acgagcaggg ccccgaccgg ttggccctgg gcaagaagat
      661 ggtccacatg ccaatgggcc tctccgggcg gaggtgccgt gccacccggg catgtagacc
      721 gttcagggcc gaaccgcagt agatgtacgt ccccgccggg aagtcctgct cccccagcgc
      781 ccctaccctg aggcggacgg gaccgtccag ggcgatgaac agcagatagc ttccgggtcg
      841 gtcctggccc atggcctccc tccccctcag gggcggtcgc accccaggac cttgccttcc
      901 acctcggcct tggtcctgat cctgatgatg gtgctgtcgc caaggcggca gtccgagatg
      961 gtggctatgt ccttcagctt atgcccgtag acctccactt cctccagctc cctcacgtgg
     1021 tccttgtacg ggatcttgac cctcagcccg tccacgtgca gcaccaccgg cgatgggcgc
     1081 aggcattcct gaacggcgtc taggtcctcg agcgcgtcct tgacctccga ggggtgcacg
     1141 aacagggggt cctcctcgat ctccttgcgg cgcctgtcca cgacctcctt gaccctgtcg
     1201 gcgatctctt cgagcttggc gatctcctcc gaacgcctca tgcgctccac cttgcgcccc
     1261 actccgcaga cgagggcctc gcagtccggg agcgagtcca gctccggacc tattacgacc
     1321 accaccggga cctggatgtc cttgtagagg ttgatcttct catgggtcac gcactccttg
     1381 aaattgccca gcatgaagat ggccgcgtcg tactcctcga ttatcgcctt ctctgccgcc
     1441 gatatctggc atgtcttccg gccccttcct ctggccaaac ccat
//
LOCUS       DAOSYN010000303         1479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_63358_length_1479_cov_3.448034, whole genome shotgun sequence.
ACCESSION   DAOSYN010000303 DAOSYN010000000
VERSION     DAOSYN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63358_length_1479_cov_3.448034"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLJ11_08275"
     CDS             complement(<1..704)
                     /locus_tag="PLJ11_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOO04700.1"
                     /translation="MALALLADGESVLRRPLLGEDTLATLEAIKAFGAQVTGGDELRI
                     VGGGLRCPDDVIDARNSGTTIRLMAGVASLLPCSTVLTGDASVRSRPMQPLIDALCML
                     GVRCESTRGNGLAPLIVRGPNLGTATEVRGDVSSQFISALLLPSPLKAVDTTVRLTSP
                     LRSRPYVDITREMMLRFGARSEALPDGFLVPGGQRYRPQTYTVPGDFSSAAFPLAAGA
                     LTGRVTVRNLDPADAQG"
     gene            complement(773..>1479)
                     /locus_tag="PLJ11_08280"
     CDS             complement(773..>1479)
                     /locus_tag="PLJ11_08280"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR01920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOO04701.1"
                     /translation="EGLPDEPTALAELCALEVLERFSVRRRAIVRTGSQLPVSRGLKS
                     SSAAANAVIMATLRALGESMPALDVLRMNADVSVRAGVSVTGAMDDAAASLLGGVVLT
                     DNHARALLRRERFGLAPRAVIGVPERMVRKAGLPRERIALHRPLVEEAFRMAWNGEYA
                     KAMYLNSLCYGAALGLDLEPPLRALAAGALGAGISGTGPAVAAFVDLGTERKVAEALG
                     MPTLAADVYQGVEGSG"
BASE COUNT          202 a          566 c          520 g          191 t
ORIGIN      
        1 ccctgggcgt ccgccgggtc gaggttgcgc acggtcaccc ggccggtgag tgcccccgcg
       61 gccagcggga aggcggcgga ggagaagtcc cccggcaccg tgtaggtctg cgggcggtag
      121 cgctgccctc ccggcaccag gaacccgtcg ggaagggcct cgctcctcgc cccgaagcgg
      181 agcatcatct cccgggtgat gtccacatag ggcctcgacc gcagcgggga ggtcagcctg
      241 accgtcgtgt ccacggcctt cagcggcgag ggcagcagga gcgcggagat gaactgggag
      301 cttacgtcgc cgcggacctc ggtcgccgtg ccgaggttcg gcccccgcac gatcagcggc
      361 gccagcccgt tcccgcgggt gctctcgcac cggacgccca gcatgcacag cgcgtcgatg
      421 agcggctgca tcgggcggct gcggacgctc gcgtcccccg tcagcacggt cgagcagggg
      481 aggagggacg ccaccccggc catgaggcgg atggtcgtcc ccgagttccg ggcgtctatg
      541 acgtcgtccg ggcagcgcag accgccgccg acgatgcgca gctcgtcccc cccggtcacc
      601 tgggcgccga aggccttaat ggcctccaag gtggccaggg tgtcctcccc cagcaggggc
      661 cgccgcagca cgctctcccc gtcggcgagc agcgccaggg ccatcgcccg gtgggtgtag
      721 ctcttggagg gggaggcggc gatgcttccc tccgccctcg agccgatgac cctcagcctg
      781 atccttccac cccctggtac acgtccgcgg ccagcgtggg catccccagg gcctcggcca
      841 ccttcctctc cgttcccagg tccacgaagg cggcgaccgc cggccccgtg ccggagatcc
      901 ccgcgcccag ggcaccggcg gccaaagccc tcagcggcgg ctccaggtcc agccccaggg
      961 ccgcgccgta gcacagcgag ttcaggtaca tggccttggc gtactccccg ttccaggcca
     1021 tccggaaggc ctcctccacc aggggccggt ggagcgcgat gcgctcccgg ggcagccccg
     1081 ccttccggac catccgctcc gggaccccga tgaccgccct gggggcgagg ccgaaccgct
     1141 cccggcggag cagcgcccgg gcatggttgt cggtgaggac gacccccccg agcagggagg
     1201 cggcggcgtc gtccatcgcc ccggtgacgg agacgcccgc ccgcacggag acgtcggcgt
     1261 tcatccgcag cacgtccagg gcgggcatgc tctcccccag ggcgcgcagc gtcgccatta
     1321 tcacggcgtt ggccgccgcg ctgctgctct tgagcccccg ggagaccggg agctgggagc
     1381 cggtgcggac gatcgccctc cgcctaacgg agaagcgctc cagtacctcc agggcgcaga
     1441 gctcggccag tgccgttggc tcgtccggaa gcccctcca
//
LOCUS       DAOSYN010000304         1471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_63842_length_1471_cov_3.295198, whole genome shotgun sequence.
ACCESSION   DAOSYN010000304 DAOSYN010000000
VERSION     DAOSYN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63842_length_1471_cov_3.295198"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..911
                     /gene="larB"
                     /locus_tag="PLJ11_08285"
     CDS             144..911
                     /gene="larB"
                     /locus_tag="PLJ11_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOO04702.1"
                     /translation="MNVRDVLEAYRQGRIDLQRAEQLLRLDFLERVGQHTLFDHAREA
                     RRGIPEVIWGENKPPSMVAEIVSTALRDRDAVLVSRASPEHVGAIIKVVDPRHVRYLE
                     GCRLIVIDRQPDRPMVGKIGILTAGSSDIAVAEEAKAMAEVMGCEVLTAYDIGVAALH
                     RFLDPLARMLDEGVDALIVVAGMEGALPTVVSGLADVPIIGVPTSVGYGFGGGGEAAL
                     MGMLQTCSPGLVVVNIDNGIAAGATAALISLRCRRKE"
     gene            916..>1471
                     /locus_tag="PLJ11_08290"
     CDS             916..>1471
                     /locus_tag="PLJ11_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO04703.1"
                     /translation="MSLVRPLRAEDVPAAQALMADTWSCLLRARTGLNVQYPVRPASW
                     YLARLRHEPLGCLCVEDEGEIIGTGFALSSGNVGWIGPMEVGPRHQGKGHGTRLLGAL
                     EAHLVGSGCGAIGLETMKDVPRNLDFYARNGYVKEQDTLYLEKGLLKPSPTRSKGEGA
                     DLEEVRRLAATINPGFDPSSEFQMH"
BASE COUNT          252 a          484 c          509 g          226 t
ORIGIN      
        1 gaagcttgcc acggcggtcc aatgcaggtc aaggcttatt ccgtggcccg tccatcaacg
       61 gtgctcatac cgcaatctcc cgaggatcgg tccctgtacc tccgcataac cgtaaaatag
      121 cccactcccc ctcccagggg acgatgaacg tccgcgacgt gttggaggcg taccggcagg
      181 ggaggatcga cctgcagagg gcggagcagc tcctgagact ggacttcctg gagagggtgg
      241 ggcagcatac gctattcgac cacgcccggg aggcccgccg cgggatcccc gaggtcatct
      301 ggggggagaa caagcccccc tccatggtcg ccgagatcgt gagcacggcg ctgagggaca
      361 gggacgcggt cctcgtctcc agggcatccc ccgagcacgt gggggcgatc atcaaggtcg
      421 tggaccccag gcacgttcgg tatttggagg ggtgcaggct catcgtgatc gaccgccagc
      481 cggaccggcc aatggtcggg aagataggca tcctcaccgc cggctcctcc gacatagcgg
      541 tggcggagga ggccaaggcc atggccgagg tcatgggttg cgaggtgctc acggcgtacg
      601 acatcggcgt cgccgccctg caccgcttcc tcgaccccct cgcacggatg ctggacgagg
      661 gcgtggacgc gctgatcgtg gtggccggaa tggagggtgc gctccccacg gtcgtctccg
      721 ggctggccga cgtgccgatc atcggcgtgc ccacctcggt cggctacggc ttcgggggcg
      781 ggggagaggc ggcgctcatg ggcatgctgc agacctgctc ccccggtctg gtggtcgtca
      841 acatcgacaa cgggatcgcg gccggcgcca ccgctgccct catcagcctc cgttgccgac
      901 gaaaggagtg aggcgatgtc cctcgtccgc cccctgcggg cggaggacgt tccggccgcc
      961 caggcgctca tggccgacac gtggtcctgc cttcttaggg cccgcaccgg cctgaacgtc
     1021 cagtaccccg tgcgccccgc ctcctggtac ctcgccaggc tcaggcacga gcccctgggg
     1081 tgcctatgcg tcgaggacga gggggagatc atcggcacgg gcttcgccct cagctccggg
     1141 aacgtgggat ggatagggcc catggaggtc ggccccaggc atcaggggaa gggccatggg
     1201 acgcggttgc tgggcgcgct cgaggcgcac ctggtaggca gcggctgcgg agcgatcggc
     1261 ctggagacca tgaaggacgt gccccgcaac ctggacttct acgctcgcaa tggatacgtc
     1321 aaggagcagg acacgctcta cctggaaaag ggactgctga aaccctctcc cacccgttcc
     1381 aaaggggaag gggcggacct ggaagaggtc cgcaggctgg cggccaccat caacccgggg
     1441 ttcgacccat cctccgagtt ccaaatgcac g
//
LOCUS       DAOSYN010000305         1464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_64239_length_1464_cov_4.645848, whole genome shotgun sequence.
ACCESSION   DAOSYN010000305 DAOSYN010000000
VERSION     DAOSYN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64239_length_1464_cov_4.645848"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..429)
                     /locus_tag="PLJ11_08295"
     CDS             complement(58..429)
                     /locus_tag="PLJ11_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HOO04704.1"
                     /translation="MDRILLRTRYDRWEIELNDTLAAFKLYEALPLEREINVWGGEIY
                     FAVPVECKLENGRKIMEEGEVAFWPEGNALCIFFGRTPVSTTSRPEAYSPVTPVGRVL
                     GGLDALAALPDRTRVTLERLD"
     gene            complement(476..1462)
                     /gene="ilvC"
                     /locus_tag="PLJ11_08300"
     CDS             complement(476..1462)
                     /gene="ilvC"
                     /locus_tag="PLJ11_08300"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOO04705.1"
                     /translation="MARIYHESDADLGVLKGKKVAVIGFGSQGKAQALCLHDSGLDVT
                     VGVRKDGKSWTDAKRNGLKVDTVAGAVKGADVVMVLIPDEVQGDVFKAEIGPNLKEGA
                     ALEFAHGFAITFGTIKPPKTVDVIMMAPKAPGPMERKVFLEGFGVPALIAVEQDHTGK
                     AKAIALALAKGLGSTKAGVLETTFREEATSDLFGEQAVLCGGVTALINAGFQTLVKRG
                     YQPEIAYFECLHEVKLIVDLIYSGGMMNMWRSVSNTAEYGGLTARDQVINEQSKAAME
                     RLLDRICSGEFAKEWLEDAQKGMPRMKALEKAESESQIEKVGAEIRALFESK"
BASE COUNT          235 a          496 c          436 g          297 t
ORIGIN      
        1 cttccgtacg gttcgacggg ttggagatag tgggaggcgc ctgacgggcg aaggccctca
       61 gtccaatctt tccagcgtca cccgggtgcg gtccggcagc gctgccagcg catctaggcc
      121 cccgagcacc cgccccacgg gcgtcacagg cgagtaggcc tcgggcctgg aggtggtgga
      181 cacgggggtc ctcccgaaga agatgcacag ggcgttccct tccggccaga aggccacctc
      241 accctcctcc atgatcttcc ttccgttctc gagcttacac tccaccggga cggcgaagta
      301 gatctccccg ccccaaacgt tgatctcccg ctccaacgga agcgcctcgt acagcttgaa
      361 ggcggctagg gtgtcgttca gctcgatctc ccatcggtcg tagcgggtgc gcaacaggat
      421 cctgtccatg ccctttcccg atgagggaaa gggttgaaaa gggaaaatgg tttgcctact
      481 tgctttcgaa gagggcccgg atctcggcgc ccaccttctc tatctggctc tcgctctcgg
      541 ccttctccag ggccttcatt cggggcatgc ccttttgggc gtcctcgagc cattccttcg
      601 caaactcccc ggagcatatg cggtccagca acctctccat ggccgccttg ctctgctcgt
      661 tgatgacctg gtcgcgagcg gtcaggccgc cgtactcggc ggtgttggag acgctcctcc
      721 acatgttcat catgcctccg gagtagatga ggtccacgat gagcttcacc tcgtggaggc
      781 actcgaagta cgcgatctcg ggctggtagc ccctcttgac cagcgtctgg aagccggcgt
      841 tgatgagggc ggtcacgccc ccgcacagga cggcctgctc cccgaacagg tccgaggtgg
      901 cctcctcgcg gaaggtggtc tccagaacgc cagccttggt ggagccgagc cccttggcga
      961 gcgccagggc gatggccttg gccttaccgg tatggtcctg ctccacggcg atcagggcgg
     1021 gaacgccgaa gccctcgagg aagaccttcc tctccatggg gccaggcgcc ttcggcgcca
     1081 tcattatgac gtccacggtc ttggggggct tgatggtccc gaaggttatc gcgaacccgt
     1141 gggcgaactc caacgccgcg ccctccttca ggttcggtcc tatctcggct ttgaagacat
     1201 cgccctgcac ctcgtcaggg atgaggacca tgaccacgtc cgcgcccttg acggcgccgg
     1261 ccacggtgtc caccttcagc ccgttccgct tggcatccgt ccaggacttg ccgtccttgc
     1321 ggacgccgac ggtcacgtcc agtccgctgt cgtgcaggca aagggcttgc gccttgccct
     1381 ggcttccgaa accgatgacc gcgaccttct tgcccttcag aacgcctaga tcggcgtccg
     1441 attcgtgata tatccttgcc atta
//
LOCUS       DAOSYN010000306         1457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_64675_length_1457_cov_3.101284, whole genome shotgun sequence.
ACCESSION   DAOSYN010000306 DAOSYN010000000
VERSION     DAOSYN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64675_length_1457_cov_3.101284"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..>1457)
                     /locus_tag="PLJ11_08305"
     CDS             complement(344..>1457)
                     /locus_tag="PLJ11_08305"
                     /inference="COORDINATES: protein motif:HMM:NF037142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HOO04706.1"
                     /translation="REAMLRELDLIKHMISVGRMREFVELRCHSSPWNVAALRVFDDD
                     HYEHQKQDFAVIGPRFYCNAKQSLVRPDVRRFRELVQSRFLRPAHRKVLLLIPCSAKK
                     PYYTSKSHQLFRQVLWEVPNSQVVQEMIITSPLGTVPRELELFYPAAQYDIPVTGHWD
                     REEQAMVRSMALSMATQGYEHVVLHLGEEGKIVREVLDGTVTSAGSPTSAEALDTLRQ
                     TLRDLCSQYERVDPGLDRRAAMSSVARFQFGPGGEALLEGARVSGSYPYLKVMRGKDQ
                     LGMLSPERGMLSLTIEGAEAILPVVPKVAIGDFDVKGSLFAVGVEDADPAIRAGEEVA
                     LVRDGAVAGVGVAMMGGREMVEMRRGEAVKVRHRRR"
BASE COUNT          255 a          480 c          469 g          253 t
ORIGIN      
        1 catcaactgg cagtgaccgg gctagcccgg tcactgtaat tttgcaacaa ggccctacaa
       61 gtgatgagaa ggtgggttca tcgttgccgg ggcatagtgc atgcggagcg cgttcgttga
      121 ggcccgtgag ctggatcgag cttcctagat gaaaaaatag gcgcaggggg gatcgggggc
      181 ttcctatccc ccttggcctt gggatggatc tcataggaac gggccgaagc ctgtgatgcc
      241 atcaacccct cgtgcatatt aaaggggaac ggggaggggg gagcgaaggt cggatcggtc
      301 ggcgaggacc tggccatacc aaccggccat tgaaagtgtg ccctcacctg cgcctgtgcc
      361 ggaccttcac cgcctcgccc cggcgcatct cgaccatctc cctcccgccc atcatggcca
      421 cgcccacccc cgccacggcg ccgtcgcgga ccagcgccac ctcctccccg gcgcggatcg
      481 cggggtcggc gtcctccacc cctacggcga agaggctgcc cttcacatcg aaatcgccga
      541 tggcgacctt ggggacgacc ggaaggatgg cctccgcccc ctcgatcgtc agggaaagca
      601 tcccccgctc cgggctcagc atccccagtt ggtccttgcc acgcatgacc ttcaggtacg
      661 gatagctgcc tgagacgcgc gcgccctcca gcagtgcctc ccccccgggc ccgaactgga
      721 atctggccac ggaggacatc gcggcccggc ggtccagccc cgggtccacc ctttcgtatt
      781 gggagcacag gtccctgagc gtctgcctga gcgtgtccag ggcctcggcg gacgtcgggg
      841 agccggcgga ggtgaccgtt ccgtccagca cttcgcggac gatcttccct tcctccccaa
      901 gatgcaggac cacgtgctcg tacccctggg tggccatgga gagcgccatg gaccgcacca
      961 tggcctgctc ctccctgtcc cagtgccccg tgaccgggat gtcgtactgc gcagctgggt
     1021 agaagagctc cagctccctg gggacggtgc ccagggggga ggtgatgatc atctcctgca
     1081 ccacctggga gttcgggacc tcccagagca cctggcggaa tagctggtgg ctcttcgagg
     1141 tgtagtaggg cttcttggcc gagcagggga tcaggagcag caccttgcga tgggcgggcc
     1201 ggaggaacct gctctggaca agctcccgga agcgccgtac gtccgggcgc acgagcgatt
     1261 gcttggcgtt gcagtagaag cgaggaccga tgaccgcgaa gtcctgcttc tgatgttcat
     1321 aatgatcgtc gtcgaacacc cgcagggcgg ccacgttcca gggggaggaa tggcagcgga
     1381 gctctacgaa ctcgcgcata cggccgaccg agatcatgtg cttgatcagg tccagctccc
     1441 tgagcatcgc ttcccgg
//
LOCUS       DAOSYN010000307         1457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_64709_length_1457_cov_2.028531, whole genome shotgun sequence.
ACCESSION   DAOSYN010000307 DAOSYN010000000
VERSION     DAOSYN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64709_length_1457_cov_2.028531"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..>1457
                     /locus_tag="PLJ11_08310"
     CDS             41..>1457
                     /locus_tag="PLJ11_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04707.1"
                     /translation="MEDGHCSFKIVKASSSFDPEVGTIKRGIATCPNPACNNVIEGDA
                     IKGEAQAGRMGHQLAAIGYKMKGRSGRHFREVTQTDLDGVVKAEEMLKKRFPEWEAKG
                     LVPTENIPEGNKTREPRNVGIMKWYQMFNPRQLLVHLTTLETIISSTKIQELDDEKRE
                     AIRTYLQISFDKCVDYNALLNTWESTRVIVKHVFQRHDYAFTWSYGEIDGAGNLFRFG
                     YSQISDAYAGICKLINGSKSNIQFLNGDASSLTSITDGSVGLIATDPPYYDNVMYSEC
                     SDFFYVWMKRGLGDVFPELFSSELTDKDLEAVANVSRFKGSSTSASKLAARDYEAKML
                     AAFREMHRVLKDDGVLNIMFTHKRVEAWDTLSHALLEAGFMITAAWPIHTESEHSLHQ
                     AKKNAAASTILLVCRKRPAEAETSWWEDLQPLLDEVVRTRAKEFASSGLRGQDTFIAC
                     FGPALQVISESWPVKKRDGTEI"
BASE COUNT          392 a          338 c          409 g          318 t
ORIGIN      
        1 cgctgaggaa ttgggatacc aacttcaatt gccggataag atggaagacg gtcattgctc
       61 attcaagatc gtcaaggcgt caagctcctt cgatccagag gtaggaacga tcaagcgtgg
      121 catagccaca tgcccgaacc ccgcctgcaa caatgtcata gagggagatg caatcaaagg
      181 ggaggcgcag gccgggagga tgggacatca gctcgctgcc atcggttata agatgaaggg
      241 aaggagcgga aggcacttcc gcgaggtcac gcagacggac ctcgacggcg tggtcaaggc
      301 cgaggagatg ctcaagaaga ggtttccgga atgggaggcg aaggggctgg taccaaccga
      361 aaatattcca gagggcaata agaccagaga gccccggaat gttggaataa tgaaatggta
      421 ccaaatgttc aatcccaggc aattgctggt tcatttgacc actttggaaa cgataataag
      481 ctcgaccaag atccaagaat tggacgacga gaagagggaa gccatacgaa cttacctgca
      541 aatttcgttt gataaatgtg ttgattacaa cgcccttctg aatacctggg aaagtacacg
      601 agtgattgta aaacatgtat ttcagaggca tgattatgca tttacctggt cgtatggaga
      661 gatagatggt gctggcaatc tgttcagatt cggttatagt caaatttctg atgcctacgc
      721 aggaatatgc aagttaatca atggttcgaa gagcaatatc caattcctga atggggacgc
      781 ctcctctctg acatcgatta cagatggaag tgtgggccta atcgccaccg atcctccata
      841 ttacgacaac gtcatgtatt cagagtgttc cgacttcttt tacgtctgga tgaagcgcgg
      901 tcttggcgat gtcttccccg aactgttctc ttccgaactg accgataagg atttggaggc
      961 agtggcgaac gtttccagat tcaaagggtc aagtaccagc gcgagcaaat tggcggcgcg
     1021 cgactatgaa gcgaagatgc tagcagcgtt cagggaaatg catcgagttc tgaaggacga
     1081 tggcgttctc aatatcatgt tcacccataa gagggtagag gcttgggaca ccctctctca
     1141 cgctctgctc gaggcggggt tcatgatcac cgccgcctgg cccatccaca ctgagagtga
     1201 acattctcta catcaggcca agaagaacgc cgccgcatcg accattttgc tcgtttgcag
     1261 gaaaaggccc gccgaagctg aaacatcttg gtgggaggac ctgcagccat tgttggatga
     1321 ggttgttaga acgagggcta aggagttcgc ctcctccgga ttgcgcggtc aagacacctt
     1381 catcgcctgc ttcgggcctg cattgcaggt catctccgaa agctggccgg tgaagaagag
     1441 ggacgggacc gagatca
//
LOCUS       DAOSYN010000308         1454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_64869_length_1454_cov_2.907791, whole genome shotgun sequence.
ACCESSION   DAOSYN010000308 DAOSYN010000000
VERSION     DAOSYN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64869_length_1454_cov_2.907791"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLJ11_08315"
     CDS             <1..241
                     /locus_tag="PLJ11_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04708.1"
                     /translation="HRDRPRGGQSPMGVLVMLLDLEDDEDRDGKFVVYFFDTNEYALV
                     PLSDLQDEAWPDRDRPYILLGPAEDWECWDDEEEW"
     gene            241..>1454
                     /locus_tag="PLJ11_08320"
     CDS             241..>1454
                     /locus_tag="PLJ11_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04709.1"
                     /translation="MLANGSVARGEVLAGDLDRSGPCATLKTVGPWELYELEGELRLT
                     RADRGLIADIALDEIKDYDKENAKLVLNMARKKPFWPLPKAIAPLKAMAEQDDPMLLA
                     DLTQFIRERVLFLNEDEYHTLAAWIAATYLKELFTKAPRWYLFGGRSSGKSTCQHWIG
                     LTAYRGIKTVNMTPPAFFRVMNRFSPTLIREESQDEKGELADAVDQVEKVGYDNDNQI
                     FRCNPNDPDIIDSFDPFGYLARSYRGERPKTDSLSRGFCVVMVNKPEEVRLKGWDDGI
                     EEAGELRARLLAFRLRALTGRVDIPGLRGSARAMAAKRFANRSLDVVVPVLATALAFG
                     EGDQVMTTAARTLRALNDATATEPEAQVFQAITRVYERQPIKFEKGTIDINGMPRTFA
                     HRDISKITTNDI"
BASE COUNT          304 a          428 c          458 g          264 t
ORIGIN      
        1 gcaccgtgac cggccccgag gcggtcaatc tcctatgggg gtactggtga tgctcctgga
       61 cctcgaggac gacgaggatc gggacggcaa gttcgtagtc tatttctttg atacgaacga
      121 gtacgccctg gtccccttgt ccgatctgca ggacgaagcg tggcccgatc gcgatcgccc
      181 atatatcctc cttggaccgg cggaggattg ggaatgctgg gacgacgagg aggagtggtg
      241 atgctcgcca atggttccgt ggctcggggg gaggtcctcg ctggggacct tgatcgttca
      301 ggtccctgcg ccaccctcaa gaccgttgga ccgtgggaac tgtacgaact cgaaggggag
      361 ctccggctga cgcgggccga tcgtggcctg atcgcagata tcgctcttga cgagatcaag
      421 gattacgaca aggagaacgc gaagctggtc ctgaacatgg cgaggaaaaa acccttctgg
      481 ccccttccga aggcgatcgc tccgctcaaa gccatggccg agcaggacga ccccatgctc
      541 ctcgccgacc ttacccagtt catccgagag cgcgtcctct tcctcaacga ggacgaatac
      601 cataccttgg ccgcatggat cgccgccacc tatcttaagg agctattcac taaagcgccc
      661 cgatggtatc tgttcggggg ccgcagctcg ggaaaatcga cgtgccagca ttggatcggg
      721 ctcaccgcct accggggaat aaagacggtg aacatgaccc cgcccgcatt cttccgcgta
      781 atgaataggt tctcccccac attgatcagg gaggagtccc aggacgaaaa gggggaattg
      841 gccgacgcgg tggaccaggt cgagaaggtg ggctatgaca acgataacca gatattcaga
      901 tgcaacccca acgacccgga catcatcgac tcgttcgatc ccttcggcta tcttgcccgc
      961 tcctatcggg gggagaggcc caaaacggac agcctttcca ggggcttctg cgtggtcatg
     1021 gtcaataagc ccgaggaggt ccggctgaag ggttgggatg atgggatcga ggaggccgga
     1081 gagctgcggg cccgcctgtt ggccttccgg ctgcgggcat tgaccgggag ggtggacatt
     1141 ccgggcctcc gaggatcggc gagggccatg gccgctaagc ggttcgccaa tcgctccctg
     1201 gacgtggtcg tcccggtcct ggcgaccgcg ctggcattcg gagagggcga tcaggtcatg
     1261 accaccgccg cgcggaccct ccgggccttg aacgatgcca cggccaccga gccggaggcg
     1321 caggtattcc aggcgatcac gcgggtatat gagcgccagc ctatcaagtt cgagaagggg
     1381 acgatcgaca tcaatggaat gccacggacc ttcgctcatc gcgatatatc caagatcacg
     1441 accaacgaca taat
//
LOCUS       DAOSYN010000309         1450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_65172_length_1450_cov_1.511828, whole genome shotgun sequence.
ACCESSION   DAOSYN010000309 DAOSYN010000000
VERSION     DAOSYN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65172_length_1450_cov_1.511828"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PLJ11_08325"
     CDS             <1..1076
                     /locus_tag="PLJ11_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04710.1"
                     /translation="ERAEVFDYVPTRNEYLNGLLMAAHTYLGGFCARLESRMRREGIE
                     REDAVLFHVHDPYLLGLATKLTKRFPRSRVVYDRHEYYETWTNRFGISVPGLFERRYG
                     KKVSEVVFVSRAIDRLPECLVDKRTTVVPNYPEAGRFDRRLAEEKIESFGKGDVLAVY
                     FGALNLDFDRDMETMFEVARCLMRSDATVRFEVAGRVFDRPLKEMMDHMEKEFGARMR
                     YLGEIPYSEVIERTQRAHLGFFLLRPESPMWSEERPVSPNKVYEFLLSGTIPVVKAVL
                     DDRAMMERCALLFDKEAAAEDIARALLALIEDKERMRGMMRGCLAIAPNFSWERVSQG
                     YLQCYERLFASMRTALESAGGNP"
     gene            1104..>1450
                     /locus_tag="PLJ11_08330"
     CDS             1104..>1450
                     /locus_tag="PLJ11_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOO04711.1"
                     /translation="MNVLSIVGARPQFIKVAPVHRAVSREHRHLILHTGQHYDYQMSQ
                     VFFEELEIPQPNYHLGVGSGSHGEQTGRMLEAIEGVLLKDRPDVVLVYGDTNSTLAGA
                     LAAVKLGVPIGHV"
BASE COUNT          285 a          400 c          484 g          281 t
ORIGIN      
        1 acgaacgggc cgaggtcttc gactacgttc cgaccaggaa cgagtatttg aacgggctgc
       61 tgatggccgc ccatacctac ctgggcggct tctgcgcgcg cctggaatca aggatgcgaa
      121 gggagggcat agaaagggag gacgcggtgc tgttccatgt ccacgacccg tacctccttg
      181 gcttggccac gaaactcacc aagcgctttc ccaggtccag ggtggtctat gaccgccacg
      241 agtactatga gacctggacc aatcgattcg ggatctcggt ccctggcctc ttcgagagaa
      301 ggtacggtaa aaaggtctcc gaggtcgtgt tcgtgagcag ggcgatcgat cgcctgcctg
      361 agtgcctggt cgataaaagg acgaccgtgg tgcccaacta tccggaggcc gggaggttcg
      421 atcgccgctt ggctgaggag aagatcgaga gctttgggaa gggcgatgtg cttgccgtct
      481 actttggcgc gctcaaccta gacttcgacc gggacatgga aacgatgttc gaggtcgccc
      541 ggtgcttgat gcggagcgat gccacggtgc gcttcgaagt tgccggtagg gtcttcgacc
      601 ggcctctgaa ggagatgatg gaccacatgg aaaaggagtt cggggcgcgc atgcggtacc
      661 ttggcgagat cccgtactcc gaggtcatcg agcgcacgca gcgcgcccat ctgggcttct
      721 tccttcttcg gccggagagc ccgatgtggt ccgaggagcg accggtatcg cccaacaagg
      781 tgtacgagtt cctcctatcg gggaccatcc ccgtggtgaa ggcggttctg gatgaccgag
      841 cgatgatgga gcggtgcgcc ctcctcttcg acaaggaggc cgcggccgag gacatcgccc
      901 gcgcccttct tgcactcata gaggataaag aacgcatgag ggggatgatg cgaggctgcc
      961 ttgccattgc accgaacttc tcatgggaga gggtgtccca aggctacctc caatgctacg
     1021 agcgcctctt cgcctcgatg aggaccgcct tggagagcgc ggggggcaac ccatgattat
     1081 caggaacgtg atagaggggc cgaatgaacg tcctatcgat agttggtgct aggcctcagt
     1141 tcatcaaggt cgctccggtg caccgggcgg tgtcgcggga acatcgccat ttgatcctgc
     1201 ataccggaca gcattatgac taccagatgt cccaggtgtt cttcgaggag ctggagatcc
     1261 ctcaacccaa ctatcatctg ggagtggggt ccggcagcca cggggagcag accggccgga
     1321 tgctggaggc gatcgagggc gtgcttctca aggaccggcc ggacgtggtg ctggtatacg
     1381 gcgacaccaa ttccaccttg gccggggcgc tggcggcggt gaagctgggc gttccgatag
     1441 ggcatgtgga
//
LOCUS       DAOSYN010000310         1449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_65184_length_1449_cov_4.004304, whole genome shotgun sequence.
ACCESSION   DAOSYN010000310 DAOSYN010000000
VERSION     DAOSYN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65184_length_1449_cov_4.004304"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PLJ11_08335"
     CDS             <1..738
                     /locus_tag="PLJ11_08335"
                     /inference="COORDINATES: protein motif:HMM:NF017570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF835 domain-containing protein"
                     /protein_id="HOO04712.1"
                     /translation="LGDSIFPDTSISLMKVPDPDALTSLVEDHRQDRAPDLAELIAHF
                     YRDNLRTEVMEGASEGGSILELKQAIIKYHRERSLEMAGKRDLRDVEVELEEGESYLV
                     EERTRDFSHGMFVSYLAKGYAGLAISRTNPRMLRRAYKEIDAELIWLTDHESEEERTI
                     PPSLEKIVVVLEEFLERQGKAVILIDDLQYLISSNSFEGALRFIRSLVDRISERPALF
                     LLSIDPASLSAQERSILEREMRLVRER"
     gene            complement(739..>1449)
                     /locus_tag="PLJ11_08340"
     CDS             complement(739..>1449)
                     /locus_tag="PLJ11_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04713.1"
                     /translation="LLILAKDYEVEVGEGRRLIDRAVQAGKQRDLGTAVQMVKEARTS
                     IRAALDARLEMEEGSLERSVGVVSRAGADPGEVAKALASLRSLREEGDIEGALQAAVK
                     GRKAAERSSAKFLEANDMAESLARLIEVCERFYLDVRDAKRMLQEARDAGDHGDWGMM
                     GILARKGREQLARSLPEVTRTEMRKAKNQLLDAKAEGKDVRTLVKVLKEAGVAMNRER
                     YDVALELLLDFRTELKRT"
BASE COUNT          229 a          517 c          452 g          251 t
ORIGIN      
        1 ctcggcgact cgatcttccc ggacacctcg atcagcctga tgaaggtccc cgacccggac
       61 gccctcacct ccctggtgga ggaccaccgg caggacaggg ccccggacct cgccgagctc
      121 atcgcgcact tctatcgtga caatctgcgg acggaggtga tggaaggggc gagcgagggg
      181 ggcagcatcc tggagctcaa gcaggccata atcaaatatc acagggagag gtcgctggag
      241 atggccggga agcgcgacct cagggacgtg gaggtggagc tcgaggaggg ggagagctat
      301 ctggtcgagg agcggacccg ggacttctcg cacggcatgt tcgtgtccta cctcgccaag
      361 gggtacgccg ggctggccat ctcccggacg aacccgcgca tgctccgccg ggcctataag
      421 gagatcgacg ccgagcttat ctggctgacg gaccatgagt ccgaggagga acggaccatc
      481 cctccctccc tggagaagat cgtggtggtg ctggaggagt tccttgaaag gcaggggaag
      541 gccgtgatcc tgatcgacga cctccagtac ctcataagca gcaattcctt cgagggcgcg
      601 ctgcgcttca tccgctccct cgtggaccgc atcagcgaac ggcccgccct gttcctgctg
      661 tccatcgacc cggccagcct cagcgcgcag gagcggtcca tcctggagag ggagatgcgc
      721 ctggtcaggg agcgctgatc aggtccgctt gagctcggtc ctgaagtcga gcagcagctc
      781 cagggccacg tcgtaccttt cccggttcat cgccaccccg gcctccttaa ggaccttcac
      841 cagggtgcgc acgtccttcc cctcggcctt ggcgtccaac aattggttct tcgccttgcg
      901 catctccgtc cgggtgacct ccggcaggga gcgggcgagc tgctcgcgcc ccttccgggc
      961 cagtatgccc atcatccccc agtccccatg gtccccggcg tcccgggcct cctgcagcat
     1021 gcgcttggcg tcccttacgt ccaggtagaa gcgttcgcag acctcgatca ggcgggccaa
     1081 ggattcggcc atgtcgttgg cctccaggaa cttggcgctg ctgcgctcgg cggccttgcg
     1141 gcccttgacc gcggcctgca acgccccctc gatgtccccc tcctccctca gggagcgcag
     1201 ggacgccagg gccttggcga cctccccggg gtccgctccc gcgcgggaga ccacgcccac
     1261 cgatcgctcc aggctaccct cctccatttc caggcgggcg tccagggcgg cccggatgga
     1321 ggtccgggcc tccttgacca tctggaccgc tgtccccagg tcccgctgct tcccggcctg
     1381 caccgccctg tctatcaggc gccgcccttc ccccacctcc acctcgtagt ccttggccag
     1441 gatgagcag
//
LOCUS       DAOSYN010000311         1441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_65674_length_1441_cov_2.758297, whole genome shotgun sequence.
ACCESSION   DAOSYN010000311 DAOSYN010000000
VERSION     DAOSYN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65674_length_1441_cov_2.758297"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..860)
                     /locus_tag="PLJ11_08345"
     CDS             complement(15..860)
                     /locus_tag="PLJ11_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO04714.1"
                     /translation="MRLTCVVDDNSGVEDPDRSIAKASGGFLSEHGLSLLVETDRGKK
                     VLMDTGSSERAFLHNLDLLGLRPKDLDMVFISHGHYDHLGGLPALLREGVPCYTHPST
                     FLGRRFIETSNRLREIGPSEGLLALLRDNPMTYDDGPRELVPGVSTTGEVQRSSSFEV
                     PSSFIIERDGRTAPDIIMEEQALCVSTKKGIVVLTGCGHAGIVNILSQVRRRSDRKIA
                     MVAGGFHLWKAPPDMLLRSIDGVKRFNVEKVAPMHCSGFAATKAFSDRFPGFELLGTG
                     CQMEL"
     gene            complement(861..>1441)
                     /locus_tag="PLJ11_08350"
     CDS             complement(861..>1441)
                     /locus_tag="PLJ11_08350"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOO04715.1"
                     /translation="RVLDKTVQAGNTSLMMSYDILVDDSGLDKLQDVANAISSKHIMF
                     ATSKVFEEIYVNEIAYWTEGMSMEMYNDMIKHAGLRPLPEIKRPKETVRLVLSDIPVI
                     GARGLRTLDDVGVYLIGSFEGCIGCKKCQKECPERALQVSPLGDRKDKRFQIKVNTEH
                     CLGTACKKCQAVCPEGVYDFTALKIVEKGADA"
BASE COUNT          258 a          472 c          416 g          295 t
ORIGIN      
        1 gcggcgcggg acctctaaag ctccatctgg catcccgtgc ccaggagctc gaacccgggg
       61 aagcggtcgg agaaggcctt cgtggccgcg aatccggagc agtgcatcgg ggccaccttc
      121 tcgacgttga acctcttgac cccgtctatg gaccttagga gcatgtccgg gggcgccttc
      181 cagagatgga accccccggc caccatggcg atcttcctat cgctccgccg ccggacctgt
      241 gagaggatgt tcacgatccc ggcgtggcca cagcctgtca gaaccacgat ccccttcttt
      301 gtcgacacgc aaagagcctg ttcctccatg ataatgtccg gggccgtgcg gccgtcccgc
      361 tcgatgatga acgaggaggg gacctcgaag ctgctgctcc gctgcacctc cccggtggtg
      421 ctcaccccgg gcaccaattc ccttggccca tcgtcatagg tcatggggtt gtccctgaga
      481 agcgccagaa gcccctccga ggggccgatc tccctcagcc tattggaggt ctcgatgaac
      541 ctcctcccca ggaatgtgga gggatgggtg tagcatggga ccccctccct caggagcgcg
      601 ggcaatcctc ctagatgatc gtagtggcca tgggagatga acaccatgtc caggtccttt
      661 ggccgaaggc ccaggagatc gaggttatgg aggaacgccc tctccgagga gccggtatcc
      721 ataagcacct tcttcccccg gtcggtctcc accaggaggg agaggccgtg ctcggagagg
      781 aaccctccgc tcgccttggc gatggagcgg tcggggtcct ccacgccgct gttgtcgtcg
      841 accacgcagg tgaggcgcac ttacgcgtcc gctcccttct cgacgatctt caatgcggtg
      901 aagtcgtaga cgccctccgg gcacacagcc tggcatttct tgcacgccgt tccgaggcaa
      961 tgctcggtgt tgacctttat ctggaacctc ttgtccttcc tgtcccctaa gggagagacc
     1021 tgcagcgccc tctctggaca ttccttctgg catttcttgc agccgatgca gccctcgaaa
     1081 gagccgatga ggtacacccc gacatcgtcc agggtccgca gcccgcgggc cccgatcact
     1141 gggatgtccg agagcaccaa ccgcacggtc tccttgggac gcttgatctc cggcaggggg
     1201 cgcaggcccg catgcttgat catatcattg tacatctcca tggacatgcc ctccgtccaa
     1261 taggcgatct cgttgacata gatctcctcg aacaccttgc tcgtggcgaa catgatgtgc
     1321 ttgcttgaga tcgcgttcgc cacgtcctgc aacttgtcca ggccgctatc gtctaccagt
     1381 atgtcatagg acatcatcag ggaggtgttg ccagcctgaa cggtcttgtc cagcactcga
     1441 g
//
LOCUS       DAOSYN010000312         1438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_65847_length_1438_cov_3.174982, whole genome shotgun sequence.
ACCESSION   DAOSYN010000312 DAOSYN010000000
VERSION     DAOSYN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65847_length_1438_cov_3.174982"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLJ11_08355"
     CDS             complement(<1..222)
                     /locus_tag="PLJ11_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO04716.1"
                     /translation="MADDWTELYRPKRLADVVGNPKVINELRQWALSWEEGRPERRAV
                     VLMGPPGVGKTSAALALANVLPARCTVIAG"
     gene            complement(300..>1438)
                     /locus_tag="PLJ11_08360"
     CDS             complement(300..>1438)
                     /locus_tag="PLJ11_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOO04717.1"
                     /translation="AYYRDDGLAYAEQNGTAWDITLLDANNELGGGISLFIDGQYRSY
                     ITYYNEISTLLKYINNTGGEWNNATVIASEGIAGVDSVISVDVDGEEHIAFVEGSGSG
                     SLNYAERRSGIWMFQKVDVDLCGENISMVMDRQGRAHVSYFDPESKDLRYTTVVSVPS
                     APTNLTVKSEDRYLELEWARPINDGGTNITEYRIYRSNVSGGPYHLLSSVDANVTTYT
                     DMGLTNGVTFYYRVKAANSEGTSPYSNEVAGTPCVLPGAPDVKASGRDGAVVLDWEEP
                     DNGGAAILKYNVYRKNATGVYHLIASVDGDKTSFRDTGLENGVEYYYYVKAVNPAGEG
                     PESDPVSATPNAGMDTAVIIGIVIAVLAVIGVGAFLLLRQRNRV"
BASE COUNT          259 a          437 c          430 g          312 t
ORIGIN      
        1 cccagcgatg acggtgcagc gcgctggcaa tacgttggcc agggccaggg ccgcactggt
       61 ctttcccacg cccggggggc ccatcagcac caccgccctg cgctcggggc ggccctcctc
      121 ccaggaaagg gcccactggc ggagctcgtt gatcaccttg gggttgccca ccacgtccgc
      181 cagccttttc gggcggtaga gctcggtcca atcgtcggcc atcatgcttc acaatgcccg
      241 cttggcttat aaaagtgagg gagtgaaaaa gaaaaaaaag gaggggccgg gccctcgttt
      301 tacactctgt tccgctgacg gaggagcagg aacgcgccca ctccgatcac ggccagcacc
      361 gctattacga tcccgatgat gaccgctgtg tccattccgg cattgggcgt ggcggagacg
      421 ggatcgcttt ccggcccctc gcctgcgggg ttgacggcct ttacgtaata gtagtattcc
      481 acgccgttct ccaggccggt gtcccggaag gaggtcttgt caccgtccac ggaggcgata
      541 agatggtaca ccccggtggc gttcttgcga tagacgttgt atttcagaat ggccgccccg
      601 ccgttgtccg gttcctccca gtcgagaacg accgctccgt cccgtccaga ggccttcaca
      661 tcaggagccc ctggaaggac gcagggcgtt ccggcaacct cgttcgaata ggggcttgtg
      721 ccttccgagt tcgccgcctt cacccggtaa tagaacgtga cgccgttggt cagccccata
      781 tcggtgtagg tggtgacatt ggcatcgacc gagctcaata gatggtatgg ccctccgctg
      841 acgttggagc ggtagatgcg gtattcggtg atattggtgc caccatcgtt gatcgggcgc
      901 gcccattcta gctcaaggta gcggtcctcg gacttcacgg tcagattggt cggggcagaa
      961 ggaacggaga ccaccgtggt gtacctcagg tccttgctct ccgggtcgaa gtagctgaca
     1021 tgggcccgcc cctgccggtc catgaccatc gagatgtttt cgccacagag atcgacatcg
     1081 accttctgga acatccagat gccgctcctc ctctccgcgt agttcaggct tccgcttccg
     1141 gagccttcga caaaggcgat gtgctcttcc ccatcgacat caacgctgat gacgctgtcg
     1201 acgccagcga tcccttcgga tgcgatcacg gtcgcgttgt tccattcgcc ccctgtgttg
     1261 ttgatgtact tgagcagggt cgatatttca ttgtagtagg tgatgtagga ccgatattgc
     1321 ccatcgatga acagggagat ccctccaccg agctcgttgt tggcgtccaa tagggtgatg
     1381 tcccatgccg tgccgttctg ttcggcatag gcaaggccgt catcgcggta gtaggcga
//
LOCUS       DAOSYN010000313         1433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_66158_length_1433_cov_4.506531, whole genome shotgun sequence.
ACCESSION   DAOSYN010000313 DAOSYN010000000
VERSION     DAOSYN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66158_length_1433_cov_4.506531"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLJ11_08365"
     CDS             <1..704
                     /locus_tag="PLJ11_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOO04718.1"
                     /translation="TDSDFPSGSGLGSSAAVTVALLAALSSAEGRLREDVVAKEAFEV
                     ECEVQGRASPIDTSTCAHGGGILVKKEGSDGLLWEIVKDTRRWCVHDIKVPAMTLVVG
                     FTGKAAPTGPLVAKVKRYVDRSTFARDIIDEIGDITLDGAAQLAKGDLEGLGRTMTKD
                     HKLLAILGVSSPELDKLVASVLPHSYGAKLTGAGGGGSMVALTDQPQKVMEVIRRKGG
                     QPFVGVTGVPGVRIE"
     gene            complement(723..>1433)
                     /locus_tag="PLJ11_08370"
     CDS             complement(723..>1433)
                     /locus_tag="PLJ11_08370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO04719.1"
                     /translation="ESQVARWAGIDTSLKTGDITTCYQYRMANLRTEPDYCEFILGSA
                     APGGYIWIFPKGDDTANVGIGVLASKLKKAGEVKRYLDAWIKKDERLSCGQPLEAVAG
                     GVSVSPPVERSVMSGLMLVGDAARIIDPITGGGIANGLLSGKYAGEVLGDCAQANDYS
                     EQALMPYDKRWRDSMENRLWRNWMAKEKFLTLSDQTLDGVIETLSTANVDKMSVHNIL
                     RAIKDRHPDLVKEFEDLI"
BASE COUNT          270 a          431 c          466 g          266 t
ORIGIN      
        1 tgaccgactc cgacttcccc tccggttcag ggctcggatc ctcggccgcg gtcaccgtag
       61 ccctcctggc cgcgctaagc tccgccgaag ggaggttgag ggaggatgtg gtcgcgaagg
      121 aggccttcga ggtggagtgc gaggtgcagg gacgagcaag ccccatcgac acctcgacct
      181 gcgcgcatgg aggcggcata ctcgttaaaa aggagggatc ggacgggctc ctctgggaga
      241 tcgtaaagga caccagaaga tggtgcgtgc atgacatcaa ggtccccgcg atgaccctgg
      301 tcgtaggctt cactggaaag gccgccccca ccggtccgct ggtggccaag gtgaagcggt
      361 acgtggaccg cagcaccttc gctcgggaca tcatcgatga gatcggggac ataacactgg
      421 atggggcggc ccagctcgcc aagggtgacc tggagggtct ggggcgaacg atgaccaagg
      481 accacaagct gctggcgatc ctcggggtat cctctcccga actggacaag ctcgtcgctt
      541 ccgtcctccc ccattcgtac ggagcgaagc tcactggggc ggggggcgga gggagcatgg
      601 tcgccctaac ggaccagccg cagaaggtaa tggaggtgat ccggaggaag ggaggacagc
      661 cgttcgtagg ggtaacgggg gtccccgggg tgaggatcga ataaaaagtg ttcttaagag
      721 gtttagataa ggtcctcgaa ctctttgaca agatcagggt ggcggtcctt gatcgcccgg
      781 aggatgttgt gcaccgacat cttgtccaca ttggcggtgg aaagggtctc gatcaccccg
      841 tccagggtct ggtcggacag ggtcaggaac ttctccttgg ccatccagtt gcgccacagg
      901 cggttctcca tcgagtcgcg ccacctttta tcgtagggca tcagggcctg ctcgctgtag
      961 tcattggcct gcgcgcaatc gcctagcacc tccccggcgt actttccgga caacaggccg
     1021 ttggcgatgc cccctcccgt gatggggtcg atgatccttg cggcgtcccc taccagcatc
     1081 agcccactca tcacggaccg ctccacaggc ggggagacgg aaacgccccc ggcaacagcc
     1141 tccaagggct ggccgcagct caatcgttcg tccttcttga tccaggcgtc caggtaccgc
     1201 ttgacctccc cggccttctt cagcttcgag gccaggacgc cgatgccgac gttggccgtg
     1261 tcatcgccct tgggaaagat ccatatatag ccgcctggag cggccgagcc gaggatgaac
     1321 tcgcagtagt ccggctcggt cctcagattg gccattcggt actggtagca ggtggtgatg
     1381 tccccggtct tcagtgaggt atcgatcccg gcccagcggg ccacctggga ttc
//
LOCUS       DAOSYN010000314         1423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_66787_length_1423_cov_3.113304, whole genome shotgun sequence.
ACCESSION   DAOSYN010000314 DAOSYN010000000
VERSION     DAOSYN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66787_length_1423_cov_3.113304"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1423
                     /gene="acs"
                     /locus_tag="PLJ11_08375"
     CDS             <1..>1423
                     /gene="acs"
                     /locus_tag="PLJ11_08375"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOO04720.1"
                     /translation="KEFSEKAYIKSMEQYREMYRQSIEDPEGFWGKIASEELHWFSKW
                     TDLHSWDKENAIIKWFEGGKLNVSYNCLDRHLENGRKNKAAIIWQGENEDEVKTYTYQ
                     QLHTEVCRFANVLRSLGVKKGDRVSIYLPMIPELAIAMLACARIGAIHSIVFGGFSSE
                     SLKDRIADCGSKILITADGNHRGSKFVPLKANADVALEKGTSIEKVIVVNRAGAECPM
                     EPGRDIWWHEEMAKADRSCEPVWCDAEDPLFILYTSGSTGKPKGVVHTTAGYLLGTCI
                     TFKYVFDYHEDETYWCTADIGWITGHSYIVYGPLCAGATTLMFEGIPTYPQVDRFWNI
                     VEKFKVNIFYTAPTAIRSLMREGEKWPQSRDLSSLRVLGSVGEPINPEAWMWYHKYVG
                     NERCPIVDTWWQTETGSVMITPLPGAITTKPGSATLPFFGVVPEVVKDDGTSAAVNEG
                     GYLCITRPWPAIMRTVWGQHERFK"
BASE COUNT          305 a          407 c          471 g          240 t
ORIGIN      
        1 aaggagttca gcgagaaggc ctacatcaag agcatggagc agtaccggga gatgtacagg
       61 cagtccattg aggaccctga gggcttttgg ggcaagatcg ccagcgagga gctgcactgg
      121 ttctccaaat ggaccgatct gcacagctgg gacaaggaga acgcgatcat caagtggttc
      181 gaggggggga agctgaacgt ttcctacaac tgcctggacc gtcacctgga gaatggccgg
      241 aagaacaagg cggccatcat ctggcagggg gagaacgagg acgaggtgaa gacctacacc
      301 tatcagcagc tgcacaccga ggtctgccgc ttcgccaacg tccttaggtc cctgggagtg
      361 aagaaggggg accgggtgtc catctatctt ccgatgatcc ccgagctggc gatagcgatg
      421 ctcgcatgcg ccaggatcgg ggcgatccat agcatcgtgt tcgggggctt cagctccgag
      481 tcgctgaagg accgcatcgc cgattgcggc tccaagatcc tgatcaccgc cgacggcaat
      541 caccggggca gcaagttcgt gcccttgaag gccaatgccg atgtggcact ggagaagggc
      601 acgtccatcg agaaggttat cgtggtcaat cgggccggcg cggaatgccc gatggagcct
      661 ggccgggaca tatggtggca cgaggagatg gccaaggccg accgctcctg cgagccggtg
      721 tggtgcgacg cggaggaccc gctcttcatc ctgtacacct cggggtccac cggcaagccc
      781 aagggagtgg tgcacaccac cgccgggtac ctgctcggga cctgcatcac gttcaaatat
      841 gtctttgatt accatgagga cgaaacatac tggtgcacgg ccgacatagg ctggatcacc
      901 gggcactcct atatcgtcta cggaccgctg tgcgccgggg ccaccacgct catgttcgag
      961 ggcatcccga cctatcccca ggtggaccgc ttctggaaca tcgtcgagaa gttcaaggtt
     1021 aatatcttct acaccgcccc gacggccatc cgctccctca tgagggaggg cgagaaatgg
     1081 ccgcagagca gggacctgag ctcactgcgg gtgctgggct ccgtgggaga gccgatcaac
     1141 cccgaggcct ggatgtggta ccacaagtat gtggggaacg aacgatgccc gatcgtggac
     1201 acctggtggc agaccgagac cggcagcgtg atgatcacgc cgctgcccgg ggctataacc
     1261 accaagccgg ggtcggccac cctgccgttc ttcggggtcg tgcccgaggt ggtcaaggat
     1321 gatggcacca gcgccgcggt gaacgagggg ggctatctat gcataacaag gccgtggccg
     1381 gccatcatgc ggacggtctg gggacagcac gagcggttca agg
//
LOCUS       DAOSYN010000315         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_67058_length_1419_cov_3.537390, whole genome shotgun sequence.
ACCESSION   DAOSYN010000315 DAOSYN010000000
VERSION     DAOSYN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67058_length_1419_cov_3.537390"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLJ11_08380"
     CDS             <1..554
                     /locus_tag="PLJ11_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04721.1"
                     /translation="GGEASFADFESIPTFESTTSKDEMIVTSKQRALEEARSDANVPH
                     VTFERVIAIPKSISRIYYPVWVVRYSYGERMYMATVDGVTGNVLSGRAPGDPLYQSLA
                     VTAGTSIGGLIAAGGLVFGLGAGEGILALIGLVVGGAVLALTYRFFRHGSEIVEGDFE
                     GKAADMRGKLMKEARRTFRGFHT"
     gene            551..1138
                     /locus_tag="PLJ11_08385"
     CDS             551..1138
                     /locus_tag="PLJ11_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04722.1"
                     /translation="MRVVQVKCPRCQQPIYSKVKDLAFYCQHCRTMHIRNGGTNIIDF
                     DIAEFGPNARGDRVYMPFWRLFSSFTINQANVAGGTFHKLSRMIKGTANGGNLFIYVP
                     AWETAVDEFKRWSMGMTEGQPMYSLRQDFNNVERGPTAITKEEAMQLADFVVVTMEAE
                     RPGTLQYLDYSLQVHDARLVYLPFVRGAQGLVPGL"
BASE COUNT          266 a          435 c          475 g          243 t
ORIGIN      
        1 tggggggcga ggcctccttc gccgatttcg agagcatccc gaccttcgag agcacgacca
       61 gcaaggacga gatgatcgtg acgtccaagc agagggcctt ggaggaggcg cgcagcgacg
      121 ccaacgtgcc gcacgtcacc ttcgagcggg tcattgcgat cccgaagagc atcagccgga
      181 tctactaccc agtctgggtc gtgcgctatt cctacgggga gaggatgtac atggcgacgg
      241 ttgacggggt gacagggaac gtgctgtcgg gaagggcccc gggggacccg ctctaccaga
      301 gcctggcggt gaccgcgggc acatccatag gtgggctcat cgccgccggg gggctggtct
      361 tcgggctcgg ggcgggagag ggcatcctcg ccctcatcgg cctggtcgtc ggaggcgcgg
      421 tccttgccct gacctaccgg ttcttccgcc atggctcaga gatcgtcgag ggggacttcg
      481 agggcaaggc cgccgacatg cgcgggaagc tcatgaagga ggctcggcgg acgttcaggg
      541 ggttccatac atgagggtcg tccaggtcaa atgcccgcgc tgtcagcagc ccatctactc
      601 caaggtcaag gacctcgcgt tctactgtca gcactgccga acgatgcaca tccgtaacgg
      661 cgggacgaac atcatcgact tcgacatcgc ggagttcggc cccaacgccc ggggggaccg
      721 ggtgtacatg cccttctggc ggctgttctc ctccttcacc atcaaccagg ccaacgtggc
      781 cggcgggacc ttccacaagc tcagccgcat gatcaagggc accgccaacg gcgggaacct
      841 cttcatctat gtccctgcgt gggagacggc cgtggacgag ttcaagcgct ggtcaatggg
      901 aatgaccgag gggcagccga tgtactccct caggcaggac ttcaacaacg tggagagggg
      961 gccgaccgcg atcaccaagg aggaggcgat gcagttggcc gacttcgtgg tcgtgaccat
     1021 ggaggcggaa aggcctggca ccctgcagta cctcgactac agcctgcagg tgcacgacgc
     1081 ccggctggtg tacctgccgt tcgtgcgcgg ggcgcaggga ttggtcccag ggctctgagc
     1141 gctccagtgc cccaccggcc ttggcccggg cccagggaat gggctggggc taccgcttcg
     1201 cgagggccag ggtagaagcc cctccctaac aacatttaat taatgttatc ctataatctg
     1261 cccaaaagtc aggtgcatcg atgtctgaga ggtccgtgca gctcgaggcg aagtggcagg
     1321 agcgctggta ttccgcgcgc atcaacgagg ccgagatgga cggccggccc aagttcatgc
     1381 tcatcttcgc ctaccccggg gtcactggct acctgcacg
//
LOCUS       DAOSYN010000316         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_67060_length_1419_cov_3.291056, whole genome shotgun sequence.
ACCESSION   DAOSYN010000316 DAOSYN010000000
VERSION     DAOSYN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67060_length_1419_cov_3.291056"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..>1419
                     /locus_tag="PLJ11_08390"
     CDS             44..>1419
                     /locus_tag="PLJ11_08390"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOO04723.1"
                     /translation="MGWNGPLEQIDAYRWMIPRSYKECMNGDAVIFADERMIRSIVSD
                     NSPEQAANVACLPGIVGRSMAMPDIHWGYGFPIGGVAAMDAEEGVISPGGIGFDINCG
                     VRLLTTALRAEEVAPRIRELIDSLFHAVPAGVGSRGVADVAANQIDRILEEGAEWAVA
                     EGYGWEEDLVRTEEGGRMGNADASKVSSKAKQRGVPQVGSLGSGNHFLEVDQVERIFD
                     PAAAKAFGLVEEGQVTVSIHCGSRGCGHQIATDYLQVMERAVRARGLRLPDRQLACAP
                     VTSQEGQDYHAAMACGANYAWANRQMIMHWVRQSFEHVFGRSAEAMGMELVYDVAHNI
                     AKLEEHVYEGRRRKVYVHRKGATRAFPAGHPEVPAIYRSVGQPVLIPGDMGAGSYVLV
                     GTEQAMRESFGSTCHGAGRLMSREAAIRSYSVKGIVQEMEAKGIYLRGSTKDGIQEEA
                     PGAYKDID"
BASE COUNT          249 a          406 c          543 g          221 t
ORIGIN      
        1 aaatcccgga agcggcatgg gatgggcgtc gaggtagatg gaaatgggct ggaacgggcc
       61 gctcgagcag atcgatgcct atcggtggat gatcccccgc tcctacaagg agtgcatgaa
      121 cggggacgct gtcatcttcg ccgatgagag gatgataagg agcatagtat cggacaattc
      181 gccggagcag gcggccaacg tggcgtgcct tcccggcatc gtcggcaggt cgatggcgat
      241 gcctgacatc cactggggct atgggttccc tatcgggggc gtggcggcca tggatgccga
      301 ggagggggtg atctcccccg ggggcatagg cttcgacatc aactgcggcg tccgcctcct
      361 taccacggcc ctgcgcgccg aggaggtcgc cccgcgcatc agggagctga tagattccct
      421 cttccacgcc gtcccggcgg gggtgggctc caggggagtg gcggacgtgg ccgccaacca
      481 gatagaccgg atccttgagg agggggcgga gtgggccgtg gccgaggggt atggttggga
      541 ggaggacctg gtgcgcaccg aggagggggg gaggatgggg aacgccgacg cctccaaggt
      601 ctcttcgaag gcgaagcaga ggggcgtgcc ccaggtcggc tcgctcggct cgggcaatca
      661 cttcctggag gtggaccaag tggagaggat attcgacccg gcggcggcga aggccttcgg
      721 gctcgtcgag gaggggcagg tcaccgtctc gatccattgc ggctcccgcg ggtgcgggca
      781 tcagatcgcc accgactacc tgcaggtcat ggagcgggcg gtgcgcgccc ggggcctcag
      841 gcttccggac cggcagctgg catgcgcgcc cgtcacctcg caggaggggc aggactacca
      901 tgcggccatg gcctgcgggg cgaactacgc ctgggccaac aggcagatga tcatgcattg
      961 ggtccggcag tcctttgagc atgtcttcgg gcggagcgcg gaggcgatgg gcatggagct
     1021 ggtatacgac gttgcgcaca acatcgccaa gctcgaggag catgtgtacg agggcaggcg
     1081 ccgcaaggtc tatgtgcatc gcaagggcgc gacgagggcg ttccccgcgg ggcatcccga
     1141 ggtcccggcc atctatcgct cggtgggcca gccggtgctc atcccggggg atatgggcgc
     1201 cggctcctac gtgctcgtgg ggacggagca ggccatgcgg gagtccttcg gctccacctg
     1261 ccacggggcg gggaggctca tgtcccgcga ggccgccatc cgctcctatt ccgtgaaggg
     1321 gatcgtccag gagatggagg ccaaggggat ctatctgagg gggagcacca aggacggtat
     1381 ccaggaggag gccccgggag cgtacaagga catagacga
//
LOCUS       DAOSYN010000317         1415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_67293_length_1415_cov_3.988971, whole genome shotgun sequence.
ACCESSION   DAOSYN010000317 DAOSYN010000000
VERSION     DAOSYN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67293_length_1415_cov_3.988971"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLJ11_08395"
     CDS             complement(<1..552)
                     /locus_tag="PLJ11_08395"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOO04724.1"
                     /translation="MSSFLDKCPIREDIIRGKLGISSLAVDLDAIVSKKHATVYDDPK
                     TFFEMTFPTRALCIIARQVFTRISGKDSTVPGIYMLGTALGGGKTHILASLYHMASGG
                     SGILSVEFKKKLADLEFPKCRVAVLTQNSPAGEKNAPRTMWGHMATQLGGYDIVKEFD
                     HNLQAPSKEALLELLEGPPTLILL"
     gene            820..>1415
                     /locus_tag="PLJ11_08400"
     CDS             820..>1415
                     /locus_tag="PLJ11_08400"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO04725.1"
                     /translation="MDENGKVAVNERSFSNVISIGAFFDHLGMTEARVVMEATGFYQY
                     LYEAIESRGYDVTLAHPLKLKAVPTGRVKTDRNDAEMLAELLRIDAVPSSYVPPKDIR
                     ALRDLTRHRSSLIAESTTLKNKMHAELARRGAKPPAEVGAAFSKRFIAWLREMRSMMT
                     DDPLDILEIVREKIKRIDERIREEANKDEDIRLLETIPG"
BASE COUNT          372 a          344 c          381 g          318 t
ORIGIN      
        1 aaggagtatt agagtaggag ggccttcaag tagttccaac aaggcttcct tcgacggggc
       61 ctgaaggtta tgatcgaact ccttgacgat atcatacccg cccaattggg tagccatatg
      121 gccccacatc gtccgagggg cgttcttttc tccagccgga gagttctggg tgagcacggc
      181 cactctacat ttagggaatt ccagatcagc caactttttc ttgaattcca ccgaaagtat
      241 tccagagcca ccactggcca tgtgataaag ggaagccagg atgtgggtct tacctccgcc
      301 aagagcggtc ccaagcatgt agattcctgg gaccgtcgaa tcctttcccg agatacgggt
      361 gaatacctgc cttgcgataa tgcacaacgc tcttgtcggg aaggtcatct cgaagaaggt
      421 cttaggatca tcgtaaacgg tcgcatgttt cttactaaca atggcgtcaa ggtccactgc
      481 aagagaagaa attcctaatt tgcctctaat gatatcttcc cttataggac atttatccag
      541 aaatgagctc aagatctcct actcccgttc gctaaaaggg ctgagatgat atatatgttg
      601 gctagaattt ttaggatatt attatttata aaactgcaat aaatattgat gcaccgaaca
      661 atcaaaataa ttcatatgat tgataccaaa aggccttgtt gatggcatgc taaaagcggc
      721 tccccgccga tacgggtgtt tgcatacatt ccgagggtgg gagaaccatg gtactatata
      781 ggtctggaca ttcataagaa gaacgctcaa gcatgcgtga tggacgaaaa tgggaaggtc
      841 gctgtcaacg agcgatcctt ctccaatgta atatcgatcg gcgccttttt tgaccacctc
      901 gggatgaccg aggcgagggt cgtcatggag gcaactggat tctatcagta cctctacgaa
      961 gcgatcgaat cgagaggata cgacgtcacg ttggctcatc ccctcaagct gaaggcggtg
     1021 cctactggga gggtgaagac cgatcggaac gacgcggaga tgctggccga gctgctgagg
     1081 atcgatgctg taccctcgtc atacgtaccg ccgaaggaca tcagggcgct gcgcgacctg
     1141 acccggcatc gttcgtcgct catcgctgaa tcgactacgc tcaagaacaa gatgcacgcc
     1201 gagctggcga ggcgaggggc gaagcccccg gccgaggtcg gggccgcgtt ctccaagcgg
     1261 ttcatcgcct ggctcaggga aatgagatcg atgatgacag acgatcccct ggacatcctg
     1321 gagatcgtga gagagaagat caagaggatt gatgaacgca tacgcgaaga agcgaacaaa
     1381 gacgaggaca tccggttgct ggagacgatc cctgg
//
LOCUS       DAOSYN010000318         1407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_67844_length_1407_cov_3.399408, whole genome shotgun sequence.
ACCESSION   DAOSYN010000318 DAOSYN010000000
VERSION     DAOSYN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67844_length_1407_cov_3.399408"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..301)
                     /locus_tag="PLJ11_08405"
     CDS             complement(146..301)
                     /locus_tag="PLJ11_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HOO04726.1"
                     /translation="MARNKPAAMKARLMKKVKQNRRVPAWVMMRTNRTFLRHPMRRNW
                     RHSKIKE"
     gene            complement(303..638)
                     /locus_tag="PLJ11_08410"
     CDS             complement(303..638)
                     /locus_tag="PLJ11_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOO04727.1"
                     /translation="MPVEDAELEELRRRKMAELQRQGEQQAVMEQQAQAIEAQRQAIL
                     RQLLTPEARDRLANVRLAYPEIARTVEDQLIRLAQMGQISSQIDDQVLKQILRKVSPQ
                     KREINIERK"
     gene            complement(638..1084)
                     /locus_tag="PLJ11_08415"
     CDS             complement(638..1084)
                     /locus_tag="PLJ11_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HOO04728.1"
                     /translation="MRLVKMVTVYDVPSEKLIANVAQKLKASGAVNPPEWAEFVKTGV
                     HTEKAPIQADWWYTRTASVLRKVYVKGPIGTSKLAAEYGGFNDRGSKPNKAVKGSRSI
                     VRKALMQLEAAGLVAKNKNNGRIVTPKGQAMLDSAAKEVSEPTPAR"
BASE COUNT          223 a          428 c          419 g          337 t
ORIGIN      
        1 acgctgttct catcggcctt catgtgccgg gcgacgtagg cgcggacctc cttcagggcg
       61 cggttggcgc gttcggtggt gggcaccgtt ttggtcttga taaggggaat gacgtacacg
      121 gactccttct cgtctgccat gcctctcact ccttgatctt gctgtggcgc cagttccttc
      181 gcatcgggtg gcgcaggaag gtgcggttgg tcctcatcat cacccatgcc ggcactcggc
      241 ggttctgttt gaccttcttc atcaatcgcg ccttcatggc ggcgggcttg ttgcgtgcca
      301 tattatttcc tctcaatgtt gatctccctc ttctgagggg aaaccttcct gagtatctgc
      361 ttcaggacct gatcatctat ctgggagctt atctgcccca tctgcgccag tcttatcagc
      421 tggtcctcca cggtacgagc gatctcggga taggccagcc ggacgttcgc aaggcggtcc
      481 ctggcctccg gcgtaaggag ctgccggagg atcgcctgcc tctgggcctc gatggcctgg
      541 gcctgctgct ccatgacggc ctgctgctcg ccttggcgct gcagctcggc catcttccgc
      601 cggcgcagtt cctcgagttc ggcatcttcc acgggcatca cctcgccggg gtgggctcgc
      661 tcacttcctt cgcggcgctg tccagcatcg cctgcccctt cggggttacg atacgaccgt
      721 tgttcttgtt cttggccact agccccgcgg cctccagctg catcagggcc ttcctgacga
      781 tggagcggct gccctttacg gccttattgg gcttggagcc gcggtcgttg aaccctccgt
      841 actcggcggc gagcttggag gtcccgatcg ggcccttgac atagaccttc ctgagcacgg
      901 atgccgtcct ggtgtaccac cagtcggcct ggatcggtgc cttctccgtg tgaaccccgg
      961 tcttaacgaa ctccgcccac tcgggagggt tcactgcccc agaggccttc agcttctggg
     1021 ccacgttcgc gatcagcttc tcggagggga catcatagac cgtgaccatc ttaaccaacc
     1081 tcaatgaaca tcagacagac gctgacgaat ggtatgttcg gcctctaata tggtgcccct
     1141 atataaggat tcttgtggtc catctccctt ctgggatgtg aataagggat gggggtgaag
     1201 gggacgaagg ctcgaagcgt tattaggatg cctttccgca cgccccggaa tggacgtagg
     1261 ggttgaaata cgcggcagtt cctactcagc cccgtgaaga cggtagtggc cataacaggg
     1321 gcgtcgggcg tcgtctacgg cgtgcgattg cttgaggagc tcgaggggga gaaggccctt
     1381 attatcaccg agacagccaa gaggata
//
LOCUS       DAOSYN010000319         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_68169_length_1402_cov_5.356347, whole genome shotgun sequence.
ACCESSION   DAOSYN010000319 DAOSYN010000000
VERSION     DAOSYN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68169_length_1402_cov_5.356347"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PLJ11_08420"
     CDS             complement(<1..1307)
                     /locus_tag="PLJ11_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO04729.1"
                     /translation="MVFELLDPRILKVLEDKDIREPTGAQSEAIPLVLAGENVLLVAP
                     TGIGKTEAAMLPLLHKLITTPGKGIQLLYITPLRALNRDMLRRLEEFGDALGLRVAVR
                     HGDTSQSERQRQSKHAPDILITTPETLQVMFTGKNLRAHLSNVRFVVVDEVHELANDE
                     RGAQLAVALERVAELAGEFQRIGLSATVGSHQEVAAFLGGVGRKVRIVRASVAKEMRI
                     TVQFPEVQEGDRDLAGTLQSDPQLIACMRRAKELIEGHRSTLMFVNTRDTAEALAARY
                     HIWDEGFKIGVHHGSLSKETRMEMEDDFKSERLKGLICTSSLELGIDIGSSDFAIQYN
                     SPRQVARLVQRIGRAGHRVGRRTEGAIIASTPDEIAESLVVTRLALAERYEPLVVRKD
                     PLTVLANQLVAMASAGPVPVEKAYALVRRAYPFRELSRQRFDAV"
BASE COUNT          217 a          498 c          439 g          248 t
ORIGIN      
        1 accgcatcga accgctgccg ggaaagctcc ctgaagggat aggcgcgccg aactagggcg
       61 taggccttct ccaccgggac cgggcccgca ctagccattg ccacaagctg attggcgagc
      121 acggtcaggg ggtccttccg gactaccaag ggctcatacc tctcggccag ggccaggcgg
      181 gtaacgacca ggctctcggc tatctcatct ggcgttgagg cgatgatggc cccctccgtc
      241 ctgcggccta cccggtgccc ggcgcggcct atgcgctgca ccagccgggc aacctgcctt
      301 ggggaattgt actggatggc gaagtcggag gagccgatgt cgatcccgag ctccaaggag
      361 gacgtgcaga tcagcccctt cagccgttcg ctcttgaaat cgtcctccat ctccatccgg
      421 gtctccttgc tcagggaccc gtggtgcacc ccgatcttga agccctcgtc ccagatgtga
      481 tagcgggccg ccagcgcctc cgcggtgtcc cgcgtgttca cgaacatgag ggtggagcgg
      541 tggccttcga tgagctcctt ggcgcggcgc atgcacgcta tcagctgagg gtcgctctgg
      601 agcgtccccg ccaggtcccg atcgccctcc tgcacctcgg ggaactgcac cgtgatgcgc
      661 atctccttgg ccacgctggc ccggacgatg cgcaccttgc gccccacccc gcccaggaac
      721 gccgccacct cctggtggga gcctacggtg gccgacagcc cgatgcgctg gaactccccc
      781 gccagctccg cgacgcgttc aagggccacc gccagctgcg ccccccgctc gtcgttggcg
      841 agctcgtgca cctcgtccac gaccacaaag cgcacgttcg agagatgcgc cctcaggttc
      901 ttgccggtga acatgacctg cagggtctcc ggcgtcgtga tcaggatgtc cggggcgtgc
      961 ttggactgcc tctgcctctc cgactgggag gtgtccccat ggcgcaccgc caccctcaag
     1021 cctagggcgt ccccgaactc ctccaggcgc ctgagcatgt cgcggttcaa cgcccgcaga
     1081 ggggtgatgt acaggagctg gatccccttc cccggcgtgg tgatgagctt gtggagcagc
     1141 ggcagcatcg ccgcctcggt cttgccgatg cccgtgggcg ccaccagcag gacgttctcc
     1201 ccggccagca ccaggggtat ggcctcgctc tgagccccgg taggctcccg gatgtccttg
     1261 tcctccagca ccttgaggat gcgggggtcc agcagttcga ataccatgtc cttcccgttc
     1321 aatatcgcgg atgtgtcacg tcgctatgcc ccggggatcc catgagaacg agcgggtgag
     1381 ggccgccagc agcacgccgt cc
//
LOCUS       DAOSYN010000320         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_68180_length_1402_cov_3.266518, whole genome shotgun sequence.
ACCESSION   DAOSYN010000320 DAOSYN010000000
VERSION     DAOSYN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68180_length_1402_cov_3.266518"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..376)
                     /locus_tag="PLJ11_08425"
     CDS             complement(152..376)
                     /locus_tag="PLJ11_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04730.1"
                     /translation="MNEDKNDFKNFGTWTRMKDLMQEIADAREFSPLDPANLDDWKQF
                     SKNPAEVQKLLDASPTYQAMLQERGFRKED"
     gene            complement(378..887)
                     /locus_tag="PLJ11_08430"
     CDS             complement(378..887)
                     /locus_tag="PLJ11_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04731.1"
                     /translation="MARPKKFESEMISKSLLLTKEQIDKAKRIGNGDFSAGIRRAVEF
                     FNEEYTDDFVRKEKERLSALMRCNELQRQKIEDDLKRFDAYKEITVRVEKNNNDIKKK
                     FLNKCVRQFQDTKLRFPNKYGYMFRGYIEGPVLEQEIISIFGSIKAAYPIIAKAVGDS
                     SWLPGVKEE"
BASE COUNT          366 a          326 c          285 g          425 t
ORIGIN      
        1 tgcgcttcca cttgatagct acatagatca agaacgggat gaacagcgtg aagcatccta
       61 tgaggaagaa tatcaccagg cccccggtgc tccatgaatg ctcggtgttc acgttacgct
      121 gacaactagg gcagaatttt attgacatat tctaatcctc cttcctgaac cctctctctt
      181 gaagcatcgc ctgatatgtt ggactggcgt ccaagagctt ctggacctct gccgggttct
      241 tggagaattg cttccaatca tcgagattcg ccggatcgag cgggctgaac tccctggcgt
      301 cagcgatctc ctgcattaga tccttcattc tcgtccatgt tccaaaattc ttgaaatcgt
      361 tcttgtcttc gttcatatca ctcctccttg acgcctggta accaagagct atcgccgacg
      421 gccttggcga tgatgggata tgcggccttg atggagccaa aaatggatat tatttcctgc
      481 tccaatactg gaccctctat atatccccgg aacatatagc catacttatt agggaatctt
      541 aacttggtat cctggaactg gcgaacgcat ttattcagga acttcttttt gatgtcgtta
      601 ttatttttct caacacgaac agttatttcc ttataagcat cgaaccgctt taggtcgtcc
      661 tcgatcttct gccgttgaag ttcgttacat cgcatcagtg cggataggcg ttccttctcc
      721 ttacggacaa aatcatcggt gtattcttca ttgaagaact cgaccgctct ccttatcccg
      781 gcagagaaat ccccatttcc tattcgcttg gccttgtcga tctgctcttt agttaaaagt
      841 aaacttttgg agatcatctc gctttcaaac ttcttaggcc gtgccaatat cgaaccctcc
      901 tcgggctgat gctggcttta tgaacattac acacccgttc atgtcgccta taaggagtta
      961 tatattattt aatgttatct taaggagagg gtggaccgga acgggtgtaa gtgtgctggc
     1021 acaccctccc ctattacgtt atacgattca gtttattaac attttcctta cgttccagga
     1081 agatgataga tagataagat attagataat agataatagg aaatgatatt atctaatatg
     1141 taactccaac cttatcgttt ttagaagttg gatatatcat cttactagaa tgatatatag
     1201 atttttgttc ttattcaaaa aagtgtcatc attttgggta ctggatgctc catcaattat
     1261 ccctcccgca aatatacaca tatagtatat atacctcatg cgtatataga ggtatcagag
     1321 gctgagaaag atggaaacct acgacgtgca cataacgacc gagaaccgcg atgggaagaa
     1381 gcaggaccag atggccatca tg
//
LOCUS       DAOSYN010000321         1401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_68295_length_1401_cov_1.661961, whole genome shotgun sequence.
ACCESSION   DAOSYN010000321 DAOSYN010000000
VERSION     DAOSYN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68295_length_1401_cov_1.661961"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..>895
                     /locus_tag="PLJ11_08435"
     CDS             258..>895
                     /locus_tag="PLJ11_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04732.1"
                     /translation="MGEGKRAYRLFNRSTRSKFIKKDRMGVASTIGTIMALMVFLTII
                     SMFTNTYIPIWMKENERDHMDEVLGQFGELKGKVDVLIVNAQTTQRPTINMFQPINLG
                     SDSVPVFTSATAGFLHLKPSGTYDTGVSVDFWYNYSGTKQHIVIDGGGCLEYYAPNRY
                     FVQQWYGYENGALIIYQKEGMAVRASPSIVLALNPDNTVNVQFDQVDLIGDN"
     assembly_gap    896..905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <906..1174
                     /locus_tag="PLJ11_08440"
     CDS             <906..1174
                     /locus_tag="PLJ11_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04733.1"
                     /translation="YMLSDPTGTHSEGTLVLKFTTRYLDALWSYINTTMLTAGLVKGV
                     ENDYTTSSKLVRAEARPVYELTLTVFDVNEFTHNRAHVKLDLEQ"
     gene            1188..>1401
                     /locus_tag="PLJ11_08445"
     CDS             1188..>1401
                     /locus_tag="PLJ11_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04734.1"
                     /translation="MESTIKKGTAKEGSAHASKPEEGGGWEAVGRAERTRNSYLKLLR
                     RMAPKRASRVMVPVRPSQGEVRPGSAV"
BASE COUNT          365 a          332 c          396 g          298 t
ORIGIN      
        1 caactgagtt actgcggctc agcccacccc ataattgagg acgattaaat aatttaccta
       61 gccgagcccg atgatggata tcgaatggaa gccttctctc gctccaccta cacctacatg
      121 gatacggcag tccgcctagt acgaccttgc aacaggatgt ctttaattgc cagctccatt
      181 ttatcgatct tgagtgttcg acatttttat atagtgaaca gcacctaaat catccaggcg
      241 gttttgaggg caatagcatg ggggagggca aacgggcata tagacttttc aacaggtcta
      301 ccaggtcgaa gttcattaag aaagaccgaa tgggcgtggc ctctaccata ggcaccataa
      361 tggccctgat ggtcttcctg acgatcatta gcatgttcac taacacctat atccctatct
      421 ggatgaaaga gaacgagcgg gaccatatgg acgaggtcct ggggcaattt ggggagctca
      481 agggaaaggt ggacgtgctc atagttaacg ctcagaccac ccagcggccg acgatcaata
      541 tgttccagcc gatcaatctc ggctccgata gcgtgccggt gtttacttcg gccaccgcag
      601 ggttcctgca ccttaaacca agtggtacat atgatacggg agtgagcgtg gacttctggt
      661 acaattacag tggaacgaag cagcacatcg tcatagacgg gggtggatgc ctagagtatt
      721 atgcccctaa caggtatttt gttcaacaat ggtacggcta tgagaatgga gcgttgatca
      781 tatatcagaa ggaggggatg gccgttcgcg cttctccctc catcgtcctc gctttgaacc
      841 cagacaatac agtcaatgtg caattcgatc aggtggacct gatcggggac aatgannnnn
      901 nnnnncatac atgctcagcg accctacggg gacccatagc gaaggaacgt tggtattgaa
      961 gttcaccacg aggtatctgg acgccctttg gtcctacatc aatacaacga tgctaacggc
     1021 agggctagtg aagggcgttg agaatgacta tacgacgagt tcgaaattgg tgagggcgga
     1081 ggcccgaccg gtatatgagc ttacattgac cgttttcgac gtcaatgagt tcacacacaa
     1141 tcgagcccat gtgaaattag acctggagca ataagaggag tggggagatg gagagcacca
     1201 taaagaaagg aacggccaag gaagggagcg cccacgcctc taagccagag gaggggggtg
     1261 gatgggaggc cgttggtagg gcagagcgga cccggaactc ctacctgaag ctccttcgga
     1321 ggatggcccc caaacgtgcc agcagggtca tggttccggt gaggcccagc cagggagagg
     1381 tgaggccggg atcggcggtg a
//
LOCUS       DAOSYN010000322         1399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_68414_length_1399_cov_2.402530, whole genome shotgun sequence.
ACCESSION   DAOSYN010000322 DAOSYN010000000
VERSION     DAOSYN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68414_length_1399_cov_2.402530"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..264)
                     /locus_tag="PLJ11_08450"
     CDS             complement(25..264)
                     /locus_tag="PLJ11_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04735.1"
                     /translation="MSDDITIKAEVKRLKEEQDRLRNMTEGFPSEVEKVLYQEAWRVM
                     GISIKEAPIDTGRLRASARVDMPIRDGNRISIRLS"
     gene            complement(257..571)
                     /locus_tag="PLJ11_08455"
     CDS             complement(257..571)
                     /locus_tag="PLJ11_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04736.1"
                     /translation="MLSGTKTWLKQTVTVKPYSSLSNYAEPSYGTGTSVSCRIQQTDE
                     QLIGEDGTLIHSTARIIVAESASVSIKDLVVLPNNEERPILKLNTIAGPDGTAYLKVL
                     YV"
     gene            complement(565..933)
                     /locus_tag="PLJ11_08460"
     CDS             complement(565..933)
                     /locus_tag="PLJ11_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04737.1"
                     /translation="MAYSTTTELKNLTGTVLTDQILSDIIAQADRTIDAKLRKAGITS
                     ANSDDLKMVSLEYAIAGLFTRYRLDGTKPSSLSLGDLSMSDTIDAAIARHLDNGDKLL
                     NDIIKGKRSYRQIVRVVNKC"
     gene            complement(935..1186)
                     /locus_tag="PLJ11_08465"
     CDS             complement(935..1186)
                     /locus_tag="PLJ11_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04738.1"
                     /translation="MPYQDMTDRELMIRLYARMDGLCEDVQELKDFLKDRPCPSELCA
                     AHGEDIAMLKYRNQLLAWSFGAVSTILAIAIAAIALVVS"
     gene            complement(1198..>1399)
                     /locus_tag="PLJ11_08470"
     CDS             complement(1198..>1399)
                     /locus_tag="PLJ11_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04739.1"
                     /translation="RLLLLAKKAMEMVELSVAGKPEYQEKLKEAKGILAMVEDAWNDS
                     KVRTPEFDELLQALFKVLAELK"
BASE COUNT          290 a          389 c          314 g          406 t
ORIGIN      
        1 taaacgaccg tggctccatt gaagctagct caatctgatg gatatcctgt tgccatcccg
       61 gatgggcata tcgaccctgg cagaagccct caatcttcca gtatctattg gcgcctcctt
      121 gatcgatatg cccattactc gccatgcttc ttgatagagc accttctcga cctcagaagg
      181 gaacccttcg gtcatattcc gtagcctgtc ctgctcctcc ttgagtcgct tcacctccgc
      241 cttgatcgtg atatcatcag acatagagca cctttagata tgccgtccca tcaggtccag
      301 cgatcgtatt caactttagg atcgggcgtt cctcattgtt tggcaggacc acaaggtctt
      361 ttattgatac gcttgcagat tctgctacga ttatcctggc tgtggaatgg atgagcgttc
      421 catcctctcc tatcaattgc tcatctgtct gctggatgcg gcatgatacg ctggtcccgg
      481 tcccgtaact cggttcggcg taattgctca atgacgaata gggcttcact gttacggtct
      541 gcttcagcca tgtcttggtg ccgctcagca tttgttcact actcgcacta tctgcctata
      601 ggatctcttg ccctttatta tatcgttcaa cagtttatct ccgttgtcca gatgccgagc
      661 tatcgcggcg tctatggtat cgctcatgga aagatcgcct agagataatg aggacggctt
      721 agttccgtca agacgatagc gagtgaacag tccagctatc gcatactcta aagataccat
      781 ttttaggtca tcgctgtttg ctgaagttat ccctgccttc ctgagcttcg catcaatagt
      841 acgatcagcc tgagctatta tgtctgaaag tatctggtcg gtcaatactg ttcctgtcag
      901 gttctttaac tcggtcgtcg tggaatatgc catatcagga caccaccagc gcgattgcgg
      961 cgatcgctat ggccaggatg gtcgataccg ccccgaacga ccaggccagc aattgattgc
     1021 gatatttcag catggctatg tcctccccat gggcggcgca tagttcggat gggcatggcc
     1081 ggtctttcaa gaaatccttt aattcctgta cgtcctcgca tagcccgtcc atcctggcat
     1141 atagccggat catcaattcc ctgtcggtca tatcctgata tggcatttta gacgctccta
     1201 ttttagctcc gccaatacct tgaacaatgc ctgtagcaat tcgtcgaact caggggtgcg
     1261 gaccttggaa tcgttccatg catcttcgac cattgccagg attcctttgg cctctttcag
     1321 cttttcctga tattccggct ttcccgctac ggatagctcg accatctcca tggccttctt
     1381 agccaacagg agcaatcgg
//
LOCUS       DAOSYN010000323         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_68561_length_1397_cov_2.049180, whole genome shotgun sequence.
ACCESSION   DAOSYN010000323 DAOSYN010000000
VERSION     DAOSYN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68561_length_1397_cov_2.049180"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLJ11_08475"
     CDS             complement(<1..527)
                     /locus_tag="PLJ11_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04740.1"
                     /translation="MGLAFLLAPTSSWTLLVSLPVLLFIPGDMAQAVFLRRLDHWEGA
                     MVAISFSALISLAIYVALSSFIGPDAYPLTYPIVGGISLALMPLVSLKGGKPLSHTCL
                     PFLLSFNWRSRSLKEKALVLFIVGCSIGTVTVGALVLSEPQAERFSEFYILNAEGKAY
                     DLPRDFVVGVGQEIR"
     gene            1132..>1397
                     /locus_tag="PLJ11_08480"
     CDS             1132..>1397
                     /locus_tag="PLJ11_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04741.1"
                     /translation="MPAGPSPDLSEVAIWTFAAALPLMVTLAAARINSTSDYALMAFA
                     VFVLCLVPLGVLCRPRSYAPLVLSLSMGLLLHRALITNYLIGYD"
BASE COUNT          300 a          364 c          406 g          327 t
ORIGIN      
        1 aatcgaatct cttggccaac gcctaccaca aaatccctgg ggagatcgta tgccttgcct
       61 tcggcattca atatatagaa ctcggagaac ctttcagctt ggggctcgct caggaccaat
      121 gcaccgacag tgaccgttcc tatcgagcat ccaacgatga acaggaccag ggccttctcc
      181 tttaaggaac gggaacgcca attgaaggag aggaggaatg gtagacaagt atgcgagaga
      241 ggtttcccgc ccttcaatga caccaacggc atcaatgcca aggatatgcc cccgacgatg
      301 ggataggtga gcggataggc atcgggaccg atgaaggaac tgagcgcgac gtagattgcg
      361 agggagatga gggcgctgaa agatatcgcc accattgccc cctcccaatg atcaagacgg
      421 cgtaggaaca cggcctgggc catgtcacca gggatgaaga ggaggaccgg tagtgagacc
      481 agcagggtcc aggatgaggt gggtgccaac aggaatgcca aacccatcat cgccaggcaa
      541 aatatcatgg cagattttgc attaagagag tacgtctggg gcggggcatc gtttgtaggg
      601 aagccgtaca atgatattta gcgatatccg catctggtga agcgaagcta cagtcgtcat
      661 tcgctaggcc tatatagtcg cttttcgatg gtaaccggcg cgatggaaga agattgttcg
      721 gtggggggaa ggcggccttt cataaatgat tggccatggc gacggcacgc cgttttgacg
      781 ttggggacgc tcgttttact aggggcactg acgattctcc gtcaagtgca cggagaggat
      841 gtatatacat ccctggccgg gctatttttg gtgcctttca tcgtactttc catcggagca
      901 gggctgctca gggtggtccc ccttcattgc atgggcttcg tccgtagcct ttttctatcg
      961 ttcgccttag gcctgggagc gctcatgctt ctcggagcgg gacttaacct attccattac
     1021 atcggtttgg tagaggaccc gttgcgcttg ggtcctatca tcgtttgtta cgctttcatg
     1081 gccttcgtca tcatgggctt ggccaggtgg cgcgaccggg atcatcgacc catgcctgca
     1141 ggcccttcgc ccgacctaag cgaggtggcg atatggacgt tcgccgctgc actgccgctc
     1201 atggtgacct tggccgctgc acgcatcaat tccaccagtg actacgcttt gatggcattc
     1261 gcggtatttg tcctgtgcct cgtacccctg ggtgtgctct gccgcccccg ttcgtacgcc
     1321 ccattggtac tgagcctgtc gatggggctg ctcctgcatc gagcgcttat aaccaattat
     1381 ttgatcgggt atgacat
//
LOCUS       DAOSYN010000324         1393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_68793_length_1393_cov_3.395366, whole genome shotgun sequence.
ACCESSION   DAOSYN010000324 DAOSYN010000000
VERSION     DAOSYN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68793_length_1393_cov_3.395366"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..377
                     /locus_tag="PLJ11_08485"
     CDS             144..377
                     /locus_tag="PLJ11_08485"
                     /inference="COORDINATES: protein motif:HMM:NF023159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO04742.1"
                     /translation="MDADEREAVLRELRTIPVVGEKVAEPLYLLGIRSVKELVGRSPE
                     DMYGELRSMKGYYVEPCMLNQLKVAVSMAAKMK"
     gene            459..>1393
                     /locus_tag="PLJ11_08490"
     CDS             459..>1393
                     /locus_tag="PLJ11_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO04743.1"
                     /translation="MQRHYFVAKMPDEPGALHRAAEIVKRHGGNIDRIQYDKRIDPCT
                     VFFEARCTDGEYMAIRSELEAIGYLQAQLRVPSFLKFQVVLPNRSGALFEFLGHTTAA
                     RCNIDFLDFDERGKHPERLTVSLTVEEAEAVDRLLEELKSVYPLEILEYDTTGQRLDD
                     TVFYIRFAQELRALIGDAEDAFLLRLLSDINHVAQELMNLGSDPRRAFSNVLLSGKGL
                     RDTSGKGFYADLQEVRLGDVELLGIQLPCGGNCYLMRRGPDVAMVDTGFGIYYRDLDR
                     LMEREGWGGVGTVRKALITHGDADHSGSAGLLSAEV"
BASE COUNT          257 a          400 c          497 g          239 t
ORIGIN      
        1 accttctgat ggggatacgg gaggaagggg gggtcggcgc gtcggtccga gggatcgtcc
       61 gcaaggcggg cgccctccgc ccctgggcca ttgggagacg gccctgaaaa cgattaaatt
      121 acgccggcct cctattcccg tccatggacg cggacgagcg ggaggctgtg ctgagggagc
      181 tgcgcacgat ccctgtcgtg ggggagaagg tggccgagcc gctgtacctc ctgggcatca
      241 ggtcggtgaa ggagctggtg ggcaggtccc cggaggacat gtacggggaa ttgcggagca
      301 tgaagggcta ttacgtggaa ccttgcatgc tcaaccagct gaaggtggcg gtcagcatgg
      361 ccgccaagat gaaatgacga gctccaatcc gatcgcatct gcgaccgcca gctttgagac
      421 ttaaattata aagggcgctc cggccttccc tctcctcgat gcagcggcac tatttcgttg
      481 ccaagatgcc tgacgagcct ggcgccctgc accgggcggc ggagatcgtc aaaaggcatg
      541 gagggaacat cgaccgcatc caatacgaca agaggataga cccatgcacc gtcttcttcg
      601 aggcccggtg cacggatggg gagtacatgg ccatccgctc cgagctggag gccatcggct
      661 acctgcaggc ccagctgagg gtgcccagct tcctgaagtt ccaggtggtg cttccgaaca
      721 ggagcggggc gctgttcgag ttcttgggcc acaccaccgc ggccaggtgc aacatcgact
      781 tcctggactt cgacgagcgg gggaagcacc cggagcggtt gacggtctcc ctgaccgtgg
      841 aggaggcgga ggcggtggac cggctcctgg aggagctgaa gagcgtctac ccgctggaga
      901 tcctggagta cgatacgacg gggcagcgct tggacgacac cgtcttctac atccgcttcg
      961 cgcaggagct gcgcgcccta atcggcgatg ccgaagatgc cttcctgctg cggctgcttt
     1021 cggacatcaa ccatgtggcc caagagctga tgaacctggg cagcgatccg agacgggcgt
     1081 tctcgaacgt gctgctgtcg gggaaggggc tgcgggacac ctccggcaag gggttctacg
     1141 ccgacctgca ggaggtgagg ctgggggatg tggagctgct cggcatccag ctgccctgcg
     1201 ggggcaactg ctacctgatg cgccggggtc cggacgtggc catggtcgat accggcttcg
     1261 gcatatacta ccgggacctg gaccgcctga tggaacggga ggggtggggt ggcgtcggga
     1321 cggtgaggaa ggcgctgatc acccacgggg acgctgacca ttccggctcg gccgggctcc
     1381 tgagcgccga ggt
//
LOCUS       DAOSYN010000325         1392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_68859_length_1392_cov_3.821242, whole genome shotgun sequence.
ACCESSION   DAOSYN010000325 DAOSYN010000000
VERSION     DAOSYN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68859_length_1392_cov_3.821242"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..582
                     /locus_tag="PLJ11_08495"
     CDS             148..582
                     /locus_tag="PLJ11_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04744.1"
                     /translation="MLPNKERDRMDQKGVGGFLESILAVMVVITASSVFLVVLSVDAV
                     PENGGPEEDEILAVLERNGLFAPVEAMELDRLRAAYVSFEPSETVSGIHLVYRLAGNQ
                     TPLLELGGFPPAEADVLSIQRPFLLLVEGRATPAVMEVSAWA"
     gene            573..1040
                     /locus_tag="PLJ11_08500"
     CDS             573..1040
                     /locus_tag="PLJ11_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04745.1"
                     /translation="MGLRKDRRGMAVLLDAFLFLLAMSLLTVMVLDVPTSGVEDDRGE
                     VLRSYHSVMLAGEMPIEGGSALSALSLAEYLVALSMSEGPLADGPIALINRAVAGTLL
                     ELTAMDERAWWELEVDGRTYVFGADVQGEVGDVHADRRELGDGKVICTLLLGG"
     gene            complement(1037..1351)
                     /locus_tag="PLJ11_08505"
     CDS             complement(1037..1351)
                     /locus_tag="PLJ11_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04746.1"
                     /translation="MKFCYCPDCKDLGPTAWYRRKECKLCGKDCQIITVPISAYGVTM
                     YALSAIGAALVVCEVFAIDLGLGTWRLYLMFGSLILAMVFSGLETGRAIEVAKERVGR
                     TL"
BASE COUNT          247 a          402 c          460 g          283 t
ORIGIN      
        1 cgctaaggcc ttcagggaga cggacatgat ggcaatggag gggcttcgcc ggaagtgtgg
       61 acggtgcagc ctcggacact accggaaggg cgttctatga ggtagcctgc gggtaatggg
      121 accttgcttc accctcctag gggcatgatg ctcccgaaca aggagaggga tcgcatggac
      181 cagaagggcg tgggtggctt cctggagtct atactggcgg tgatggtcgt catcaccgcc
      241 tcctccgtgt tcctggtcgt cctatcggtg gatgcagttc cggagaatgg gggccctgag
      301 gaggatgaaa ttcttgcggt gctcgaaagg aacggactgt tcgccccagt ggaggcgatg
      361 gaactggacc gcttgcgcgc cgcatacgtc tccttcgagc cttcggaaac ggtctcaggg
      421 attcatctcg tttacaggct ggcggggaac cagacgccgt tgctggagct aggagggttt
      481 cctcctgccg aggccgatgt cctatccatc cagaggcctt tcctcctttt ggtcgaggga
      541 agggccaccc ctgcggtgat ggaggtcagt gcatgggcct aaggaaggac cgccggggga
      601 tggcggtgct cctcgacgcg ttcctcttcc tactggcgat gtccctgctt accgtcatgg
      661 tgctggatgt ccctacgagc ggcgtcgagg acgatagggg cgaggtgctg cgctcctacc
      721 actccgtgat gttggccggg gagatgccca tcgaaggagg gagcgccctt tccgccctct
      781 ccctggccga atatctggtc gctctttcca tgagtgaggg gccgcttgcg gatggcccca
      841 tagccttgat caatagggcc gtcgccggca cgctcctgga gctgaccgcc atggatgagc
      901 gcgcctggtg ggaactcgag gtcgatggcc gaacgtacgt gttcggcgca gatgtccaag
      961 gcgaggttgg ggacgtgcat gccgaccgca gggagctggg cgatgggaag gtgatatgca
     1021 cgctactgct cggaggttag agcgtcctcc cgaccctttc cttggccact tcgatggccc
     1081 gtcccgtctc gagccccgaa aacaccatgg ccaggatcag gctgccaaac atcaggtaca
     1141 ggcgccaggt cccaagcccc aggtcgatag cgaagacctc gcagaccacc agggccgctc
     1201 cgatcgcgct cagcgcgtac atggtcaccc cataggcgga gatggggacc gtgattatct
     1261 ggcagtcctt accgcacagc ttgcactcct tgcgccgata ccatgctgtc ggtccaaggt
     1321 ccttgcaatc cgggcagtag cagaacttca tgagggggca tccggctggt cggggttaat
     1381 catttccctt tc
//
LOCUS       DAOSYN010000326         1390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_69003_length_1390_cov_5.740075, whole genome shotgun sequence.
ACCESSION   DAOSYN010000326 DAOSYN010000000
VERSION     DAOSYN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69003_length_1390_cov_5.740075"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..560)
                     /locus_tag="PLJ11_08510"
     CDS             complement(225..560)
                     /locus_tag="PLJ11_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04747.1"
                     /translation="MSNDEEFVKMVDKMLKDYVRMADLPPWDKIEYNAPTKAVGFTMY
                     FRRTDNPDAVLDQLDDKIAMCSYVNKKLAGEIVERPKLNAPFVITDDMDDDQAILSIE
                     EQLKRGGKQ"
     gene            complement(557..739)
                     /locus_tag="PLJ11_08515"
     CDS             complement(557..739)
                     /locus_tag="PLJ11_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04748.1"
                     /translation="MQPTYVNYGVVLSIDVEQIGELKSLIKDLGAKMIYQKVSSEHLT
                     IIEKSKLNDEEKEELE"
     gene            complement(757..1161)
                     /locus_tag="PLJ11_08520"
     CDS             complement(757..1161)
                     /locus_tag="PLJ11_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04749.1"
                     /translation="MTQIKAIAVKGWTDRTTQKLDYTLELVADYDYLPMTEEQDRLLI
                     IEANHIKGVLDNLLMTWKNAPTGEFKTAETTVPTKEQKPAEPNEPGLVLCSICKSSTE
                     ERISVKGVPYRYCPQCKDTRRLDGTPFPPKKK"
BASE COUNT          302 a          355 c          292 g          441 t
ORIGIN      
        1 ccatgcggcg aatgtctgaa ctccctatcg ctcattctat cacatttttt atttcgagag
       61 tgcgttcctc atcaggacat attgcggaat cctacagatc gaacagtccc cattctcgca
      121 cattatcctg gaatcgcccc aggaaaactg gcacagatcg aacattctat gcgcttcgtc
      181 attgaacacc tgctcccgaa acagattttc tttgtataat attttcattg cttccccccg
      241 cgcttcaatt gttcctcgat ggataggatc gcttggtcgt catccatatc gtccgtaatg
      301 acgaagggag cattgagctt gggccgttct acgatctccc cagccaactt cttgttcaca
      361 taagagcaca tggctatttt atcgtccaat tggtctagga cggcatcggg attgtcggtc
      421 cgtcggaaat acattgtgaa ccccacagcc ttcgtgggag cgttatattc gatcttatcc
      481 caaggcggaa gatcagccat tctgacgtaa tccttgagca ttttatctac catcttaacg
      541 aattcttcat cattgctcat tccaactcct ccttttcctc gtcattcagc ttggatttct
      601 cgatgatcgt cagatgctcg ctggatactt tctgatagat catcttggcc cccaagtcct
      661 ttatcaagga cttgagttcg cctatctgtt cgacgtctat ggataatact actccatagt
      721 ttacgtatgt tggttgcatt cgttttcctc ctatcgttac ttcttctttg gcgggaacgg
      781 agttccatct aaccgccttg tatccttgca ttgggggcaa tatcggtaag gtacaccctt
      841 tactgatatc cgctcctcag tgctggactt gcatatagaa caaagcacaa gccccggctc
      901 attaggttct gctggcttct gctccttcgt tgggactgtt gtttcagcgg tcttgaactc
      961 gccggtaggc gcgttcttcc aggtcatgag caggttatcg aggacgcctt taatatggtt
     1021 cgcctcaatt attaaaagtc tgtcctgctc ctcggtcatc ggcagataat catagtccgc
     1081 tacgagctct aaagtataat caagcttttg ggtggtccta tcggtccacc cctttactgc
     1141 gatcgctttt atttgcgtca ttcttttttc ctccttattg ttgattgttg atacattccc
     1201 tcgcagatgg aagcgatatg ctccagctca caggaaatcg tcgtccatac gaacgaccaa
     1261 ttcaggaagg aatgggtggg aatcctgcga gggaatgtac aataggatat tgtcttgagg
     1321 gtatatattg attatcattc ttcagttcaa tcctttatat tctctcgact cgctagtcag
     1381 gtggacggcg
//
LOCUS       DAOSYN010000327         1387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_69252_length_1387_cov_3.027778, whole genome shotgun sequence.
ACCESSION   DAOSYN010000327 DAOSYN010000000
VERSION     DAOSYN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69252_length_1387_cov_3.027778"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1306
                     /locus_tag="PLJ11_08525"
     CDS             131..1306
                     /locus_tag="PLJ11_08525"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO04750.1"
                     /translation="MDSRRLTMFIGGYIGPLASNAVLAMVPVLKAEFGAGAEDVLLAV
                     TFYMLPFALFNLISGTVSDIYGRRKVLTFGFLLYALSNVLCALSPNLPAFYLFRSMQG
                     IGYAFANPVLMAVLGDITPPEERGRAMGYFGAFTTAGIATGPMIAGLLIPYGWRWTFM
                     LVAVLALGVIVWIRAVCPAVRPDMEAFRHLKENVEAAVRTRGMAALCLLGFLTFLCYM
                     GALGFLSDHLSLPPLSLDERTIGVMVGMSGVAGIVAAPIGGRLVDGRGRTRTALTGFL
                     TMALALSLLYLSREPLQFAVSLLVLGIGTAFIWTALLTLAVEIVPGLKGTTSSIFNSA
                     RFFGYSLAPLLFAPIYSFQGFGTVLLVGLGLTALALLVVRVLAAQLNPSHSTSTRSS"
BASE COUNT          200 a          488 c          407 g          292 t
ORIGIN      
        1 ctcccttcca tagctatcgc ccatactatc ccgagcccgt agaaatacgt tgcctcagga
       61 caagggaaca tccacggcgt tcccctcgcc ggagatttcc ttattatcgg accagcctct
      121 tcctatggca atggacagca ggcggctgac gatgttcata ggcgggtata tcggccctct
      181 ggccagcaac gccgtcctgg ccatggtccc ggtcctcaag gccgagttcg gggcaggggc
      241 ggaggatgtc ctgctggccg tcaccttcta catgcttccc ttcgccctct tcaacctgat
      301 ctccgggacg gtgtcggata tctacgggcg gcggaaggtc ctcaccttcg gcttcctgct
      361 ctacgcccta agcaacgtcc tgtgcgcgct cagcccgaac ctgcctgcct tctacctatt
      421 ccgttcgatg caggggattg gatacgcctt cgcgaatcca gtgctgatgg cggtgctggg
      481 cgacatcacg ccgcccgaag aaagggggag ggccatgggc tacttcgggg ccttcacgac
      541 cgccgggatc gccaccggtc cgatgatcgc cggtctcctc atcccctacg gctggaggtg
      601 gacgttcatg ctcgtcgccg tcctcgccct cggcgtgatc gtctggataa gggccgtctg
      661 ccccgccgtc cggccggaca tggaggcctt caggcatctc aaggagaacg tggaggccgc
      721 cgtgcgcact aggggcatgg cggccctgtg cctcctcggc ttccttacat tcctgtgcta
      781 catgggcgcc ttgggcttcc tgtccgacca cctttcgctg cccccgctct cgctcgacga
      841 acggacgatc ggggtgatgg tgggcatgag cggcgtggcc ggcatagtgg ccgccccaat
      901 cgggggcagg ctcgtcgacg gaaggggcag gacgcggacg gcgctaactg gcttcctcac
      961 catggccttg gccctctccc tcctctatct atcgagggag cccctgcagt tcgctgtctc
     1021 gctgctggta ctcggtatcg gcactgcctt catctggacc gcgctgctca ccctggcggt
     1081 ggagatcgtt ccgggactga agggcaccac gtcctctatc ttcaacagcg cccgcttctt
     1141 cggctattcc ctcgcccccc ttctcttcgc ccccatctac tccttccagg gcttcggcac
     1201 ggtgctcctg gtgggcctgg ggctcaccgc cttggccctg ctggtcgtcc gcgtactggc
     1261 ggctcagctc aatccgtccc attcaaccag cacccgctcc agctgagagc ggacctcgtc
     1321 catggggccg cggttgtcca ggaccttcca gcctcgggag gagagggcga gcaccttccc
     1381 cctcacc
//
LOCUS       DAOSYN010000328         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_69829_length_1378_cov_5.708995, whole genome shotgun sequence.
ACCESSION   DAOSYN010000328 DAOSYN010000000
VERSION     DAOSYN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69829_length_1378_cov_5.708995"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1378)
                     /locus_tag="PLJ11_08530"
     CDS             complement(<1..>1378)
                     /locus_tag="PLJ11_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOO04751.1"
                     /translation="VEVFIHGAQCYCFSGQCLFSSFHGGRSGNRGMCAQPCRKRYSLG
                     NREGYLLSTADMFAIDAIPALMRSGVKALKIEGRMRSPQYVYLTSMMYRRAIERAREG
                     TSPLITERERELLEVVFNRGFSGGYLKDKELIQPSYPESRGRPLGTASVKDGRLEMPR
                     HTLCPSDGISLYRGDVKVGGFDLKPSDLQVDVVQAVPPFPLADGVYAVYKTKDRELPA
                     IEAAVSKIKFQEGEAVRRAVELPTRRMRRGERESELSFYVSSVAVLKAVLPYADRIYF
                     DGTKQMAEATRLCEGEGKELVQMLPRLSLDEARVESTAVMVHTIDQYELHKDRRVYGS
                     YHMNVFNSHTLPRMWQWTLSPELTREEMFEVLSHTDQRCEVMAFGRLELMLSRDPTLP
                     EGTLIDERGVRYPVYRDKEGFVHILHSADLLLLDKVPELRAMGVDSLGIDVRRRHPDL
                     AAFVAKAFR"
BASE COUNT          257 a          444 c          395 g          282 t
ORIGIN      
        1 cctgaaggcc ttagcgacaa acgccgccag gtccggatgg cgccttcgga cgtcgatgcc
       61 tagtgagtcc actcccatcg ccctcagctc ggggaccttg tccagaagga gcagatcggc
      121 gctgtgcagg atatggacga agccttcctt gtcacggtat acgggataac gcaccccccg
      181 ctcatcgata agggtcccct cgggcagggt cgggtcccgg ctgagcataa gctccagccg
      241 tccgaaggcc atgacctcgc acctttgatc tgtatgggaa agaacctcga acatctcttc
      301 gcgcgtgagc tcaggggaca atgtccactg ccacatcctc gggagcgtgt ggctgttgaa
      361 cacgttcatg tgataggagc catagaccct gcggtcctta tggagctcgt actgatcgat
      421 cgtgtgtacc atgaccgcgg tgctctccac ccttgcctca tcgagggaga ggcgggggag
      481 catctggacc aattcctttc cctccccttc gcagagccgc gttgcctccg ccatctgctt
      541 tgtaccatcg aagtatatcc ggtccgcata gggcagcacg gccttgagga cggctacgct
      601 ggacacgtag aaggaaagct cgctttcccg ttcccctcgg cgcatacgcc tggtcggcag
      661 ctccaccgcc ctcctgacgg cctccccctc ctggaatttg atcttcgaga cggcggcctc
      721 gatggccgga agctccctgt ccttggtctt gtagacggcg tacactccat cggcaagggg
      781 gaagggcggg accgcctgaa ccacatcgac ttggagatcg gagggcttga ggtcgaaccc
      841 tccaaccttc acatccccgc gatagaggct gatgccgtcc gaggggcaga gggtatggcg
      901 aggcatctcc agcctgccat ccttcacgga ggcggttcct agcggtctcc ccctggattc
      961 ggggtaggag ggctgtatta actccttgtc cttgaggtat cctccggaga agcctcgatt
     1021 gaagactacc tccagcaact ccctctcgcg ctccgtgatg agaggggatg tgccttccct
     1081 cgccctctcg atcgccctcc tgtacatcat ggatgtgagg tacacgtact ggggcgaacg
     1141 catccttcct tcgatcttca aggccttcac cccggagcgc atcagcgcag ggatggcatc
     1201 tatggcgaac atgtccgccg tgctgagcag gtagccctcc ctattcccga gcgaatacct
     1261 cttccggcag ggctgggcgc acatccccct gttcccggag cgtcccccgt ggaagctgga
     1321 gaaaaggcat tgcccggaga agcagtagca ttgcgctcca tgaatgaaga cctccacc
//
LOCUS       DAOSYN010000329         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_70487_length_1369_cov_4.282344, whole genome shotgun sequence.
ACCESSION   DAOSYN010000329 DAOSYN010000000
VERSION     DAOSYN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70487_length_1369_cov_4.282344"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PLJ11_08535"
     CDS             <1..1213
                     /locus_tag="PLJ11_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO04752.1"
                     /translation="EGKGMNALQAAAQGSLVGAMAGLIALLLLPFVHAEAGEALERIS
                     PLVPWILLLIPFLLFWAESGKADPKAQLDMRAGRAMPTNVISLKRHVPVHGDSARVIG
                     TVRKRRGRWTVWTPAQSFVIRGRGLKEGHQLLLGQWTVRRDRLPLIGWSALLFLCSGA
                     LGIMAMDGLVPGQDIWTGMEGNMLMPLFTGLFGLPLLIGSSRGRMPEQSGEAGAPPSV
                     TGALKGSFMGGLVGWLPGVTATAGAVLTTMLPRSRAGPEEFICTVSAVGTGAAVLGLG
                     TLVLLGKGRSGTLLAMQGVVPSLSVEDLPALFLCILISSLVSYSLTMRLGRRLLLQAQ
                     GRDLTALNKGALVGMVVLSFLLAGPGGLLLLLLSTLLGMVPGRVGVSRVHLTGCLLFP
                     ILLHYFAPFLR"
     gene            complement(1189..>1369)
                     /locus_tag="PLJ11_08540"
     CDS             complement(1189..>1369)
                     /locus_tag="PLJ11_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04753.1"
                     /translation="EFVLTPGKEVKVAQFLEGTEGYDAGRYEELLFRAAGELLSPFDL
                     GQVRLGEFYRRNGAK"
BASE COUNT          181 a          477 c          458 g          253 t
ORIGIN      
        1 ggagggaaag gggatgaacg ccctgcaggc ggccgcccag ggaagcctgg tgggggccat
       61 ggccggtctc atcgccctgc tccttctccc tttcgtccac gctgaggccg gggaggccct
      121 ggagcggatc tcccccttag ttccgtggat actgctgctc atccccttcc tcctcttctg
      181 ggcggagagc gggaaggccg atcccaaggc gcagctggac atgcgcgccg gccgtgcgat
      241 gccgaccaat gtcatctcct tgaagaggca cgtcccggtg cacggcgact ccgccagggt
      301 gatcgggacc gtgcgcaagc ggcggggaag gtggaccgta tggacgcccg cgcagtcctt
      361 cgtcatccgc ggcaggggat tgaaggaagg ccatcagctg ctcctaggtc aatggaccgt
      421 ccgcagggac aggctgccgc tcatcggctg gtccgcgctc cttttcctgt gctccggcgc
      481 cctgggcatc atggccatgg acggccttgt gccgggccag gacatctgga cggggatgga
      541 gggcaacatg ctcatgcccc tcttcaccgg gctcttcggc cttcccctgc tcatcgggtc
      601 ctcccggggc cgcatgcctg agcagagcgg agaggcgggc gcgcccccgt ccgtgaccgg
      661 ggcgctgaaa gggtccttca tgggcgggct ggtgggatgg ctgccgggcg tcacggcaac
      721 ggccggcgcg gtactgacca ccatgctccc ccgctcccgc gcggggccgg aggagttcat
      781 ctgcaccgtg tccgcggtgg gcaccggggc ggcggtgctc ggcctgggga cccttgtcct
      841 tctcgggaag gggaggagcg gcacgctgct tgctatgcag ggggtcgtgc cctcgctctc
      901 ggtcgaggac ctgccggcgc tgttcctctg catcctcata tcgtcccttg tatcatactc
      961 cctcaccatg cgcctgggca ggaggctcct cctgcaggcc caggggcggg accttacggc
     1021 gctgaacaag ggcgcgctgg tcggcatggt cgtcctctcc tttctcctgg caggcccagg
     1081 tggcctgctg ctcctgctgc tctccaccct cctggggatg gtgcctggaa gggtcggcgt
     1141 cagcagggtg cacctcaccg gctgcctgct gttccccatc ctccttcact atttcgcccc
     1201 gttcctgcgg tagaactccc ccaatctgac ctggccgaga tcgaagggcg agagcagctc
     1261 gccggcggcc cggaacagca gttcctcgta ccgtccggcg tcgtacccct ccgtcccctc
     1321 caggaactgc gccaccttca cctccttccc gggggtcagg acgaactcg
//
LOCUS       DAOSYN010000330         1359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_71295_length_1359_cov_2.399540, whole genome shotgun sequence.
ACCESSION   DAOSYN010000330 DAOSYN010000000
VERSION     DAOSYN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71295_length_1359_cov_2.399540"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PLJ11_08545"
     CDS             complement(<1..1034)
                     /locus_tag="PLJ11_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04754.1"
                     /translation="MSLLLLTVMIYGPGIAFYLSDTSVHYDYDLYRARTQTILDGGLL
                     YRDVPTETPPLINYLLVPAQLLGGAESPYVWGAYEAFFAFLLAGMLYLAFRRYDEWKA
                     YLMGVITVLSPYLIIESATGEDAAIVAFVFFLGAAAMALNSRYAPMAIALGIWTKMWS
                     VLMFPIEFLRAKGLREKAEVVVVVGIITLVIAFPFLALCGDEFLAFLSFYFLGDPSRP
                     SGGRSIWNFLRDGGYGVPATVELALVLGSLLVAYLYSYHRGWGPWRSITLAMMAFMVF
                     YPKMHMGYYVMPFVLLAAWAVSDWRVVARLFLAYVPITFSGGFASDNPNPIIAPAEGG
                     WLIGLLLVLIG"
BASE COUNT          328 a          423 c          369 g          239 t
ORIGIN      
        1 ccgatgagga ctagcaaaag gccaatgagc cagcctcctt ccgcaggggc tatgatgggg
       61 ttcggattgt ccgaggcaaa gccgcctgag aacgttatcg gaacgtaggc aaggaacaag
      121 cgagcgacga ccctccagtc gctgaccgcc caggccgcca aaaggacgaa gggcatcacg
      181 tagtacccca tatgcatctt ggggtagaag accatgaacg ccatcatggc cagggttatt
      241 gaccgccaag gcccccatcc cctatgataa ctgtacagat aggcgacgag cagcgagccc
      301 aaaacgagcg ccagctcgac ggtggcaggc actccatagc cgccatccct cagaaagttc
      361 cagatgctcc ttcctccgga aggtctcgac ggatccccaa ggaaatagaa ggagaggaat
      421 gccaggaact catctccgca gagcgcaagg aacgggaagg cgatcaccaa ggtaatgatc
      481 cccaccacaa ccaccacctc cgccttctcc ctcagccctt tggcgcgaag gaactcgatc
      541 gggaacatga gcaccgacca catcttcgtc caaatgccca aggcgatggc cataggggcg
      601 tacctgctat tcagcgccat ggccgcagcg cccaggaaga agacgaaggc cactatggct
      661 gcgtcctccc cggtggcgga ctcgattata aggtaggggg agaggacggt gatgaccccc
      721 ataagatagg ccttccactc atcatagcgg cggaaggcaa gataaagcat gcctgccagg
      781 aggaacgcga aaaaggcctc gtaagccccc cacacgtagg ggctttccgc tcctcccagg
      841 agctgcgcgg ggaccaatag gtaattgatc agtgggggcg tttcggttgg cacatcccgg
      901 tagagcaatc ccccatccaa aatcgtctgg gtcctggccc ggtagaggtc ataatcataa
      961 tggacggagg tgtccgaaag gtagaacgcg atccccgggc cgtatatcat gacggtcaga
     1021 agcagtagcg acaagacctt ttgccagcgg ctcatcgctc ggattgatga aaggtgccgc
     1081 gcgatccttc gacccagggc cgtccttatc ttttctgttg caaacgcatg cattcccttc
     1141 gccgttcctc tccaatcgat gccaaggcca aaggcaatca tcctagaccg gcgaactatt
     1201 atttctcgat tgacccagtc tcaccccctt cctttgcagc cgaccagcga gccggcttca
     1261 ttcatccctc ccgtccaaat gcggggcgaa cccctccgct tgcaagtttg gacggaccct
     1321 cgtctcgaaa cagcccgtgc cggtgacctt gaccgctcc
//
LOCUS       DAOSYN010000331         1358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_71345_length_1358_cov_2.492709, whole genome shotgun sequence.
ACCESSION   DAOSYN010000331 DAOSYN010000000
VERSION     DAOSYN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71345_length_1358_cov_2.492709"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLJ11_08550"
     CDS             complement(<1..715)
                     /locus_tag="PLJ11_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HOO04755.1"
                     /translation="MNTEIRRVQKTGASTLTVSLPKDWADLSGLKPGDQVAMLVQTDG
                     TIVLDPRSDRRKEALRKEIWTDGAESAEHLKRKLIGAYLAGFNVIQVRSKERMEPEIK
                     GAIREFSRLAIGPEVVEETANSVLLHDLSDPVELPQEKCVRRMHLIVRAMHADAIEAL
                     CQEDKGLAEDVIDRDTDVDRLYWMAVKQNTLIARDRRLGERIGQDIFGSNDLMLVARG
                     VERIGDHAVRIARNALLSAE"
     gene            1014..>1358
                     /locus_tag="PLJ11_08555"
     CDS             1014..>1358
                     /locus_tag="PLJ11_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO04756.1"
                     /translation="MENRMKYLVIGVVAIIIVAAVGVLLFQGNDDDDGEGGVIAQKGS
                     DTMLELCQIWAEEYMAENPDVMIEVSGGGSGTGITALINGQVDIAQASRQIKASEIEA
                     AQANGVNPVEFRV"
BASE COUNT          274 a          416 c          370 g          298 t
ORIGIN      
        1 cctccgcgct cagcaacgcg ttccgggcga tgcgcacggc atggtcccct atgcgctcca
       61 cgccgcgggc gaccagcatg aggtcgttgg agccgaagat gtcctgccct atacgctccc
      121 cgagcctccg gtccctggcg atgagggtgt tctgcttcac cgccatccag tacagccggt
      181 ccacgtccgt gtcccggtcg atgacgtcct ctgccagccc cttatcctcc tggcagagcg
      241 cctcgatggc gtcggcgtgc atggcccgca cgatcaggtg catcctccgc acgcacttct
      301 cctgggggag ctccacaggg tccgagaggt catgcaacag cacgctgttc gccgtctcct
      361 ccaccacctc cggcccaatg gccaggcgtg agaactccct gatggcgccc ttgatctccg
      421 gctccatgcg ctccttggag cgcacctgaa tgacgttgaa gccggcaagg taggctccga
      481 tgagcttcct cttcaaatgc tcggcgctct ccgccccgtc cgtccagatc tcctttctca
      541 aagcctcctt gcggcggtcg ctgcgcgggt ccaggacgat cgtcccatcc gtctggacca
      601 gcatggcgac ctggtcgccc ggcttcaggc cggagaggtc cgcccagtcc ttaggcaggg
      661 aaacggtcag tgtggacgcc ccggtcttct ggaccctgcg gatctcagta ttcattctct
      721 tatcactatt gtcccggttc acaaccatac ttaagtgttt attgtatata ctaaacttac
      781 taaaaatata aaaaattcct gttggtattt tttctatata taaaatacaa gaatatattc
      841 tatttcgcta atgtatatga acagcgccct agtacttcgc tccggagaaa atgcacaaag
      901 agccggtcgg gacacgaaac atgggcatcg tacgcaactc aatcctccgc ttctcgccct
      961 gtatgactgg accgcggctc tttgttcttt ctctacattt caaaggtgaa tgaatggaaa
     1021 acagaatgaa gtacctggtc atcggcgtcg tagccataat catagtggcc gctgtcggtg
     1081 tgctcctctt ccagggcaat gatgatgacg acggtgaggg cggcgtcatt gcgcagaagg
     1141 ggtccgatac gatgcttgag ctctgccaga tctgggcaga ggaatacatg gctgagaacc
     1201 ccgatgtcat gatcgaggtc tccgggggcg gaagcggcac cggcatcacc gccctgatca
     1261 acgggcaggt ggacattgca caggcctcta ggcaaataaa ggccagcgag atcgaggccg
     1321 cccaggccaa tggcgtgaac cccgtggagt tcagggtg
//
LOCUS       DAOSYN010000332         1349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_71958_length_1349_cov_3.598918, whole genome shotgun sequence.
ACCESSION   DAOSYN010000332 DAOSYN010000000
VERSION     DAOSYN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71958_length_1349_cov_3.598918"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLJ11_08560"
     CDS             complement(<1..1000)
                     /locus_tag="PLJ11_08560"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO04757.1"
                     /translation="MSPQVTFHPKGRSIDVPEGTSLLEAAILAGVEVNSACAGKGVCG
                     RCKVIVEGDVESEPSAKVTGEEWSTGYRLACRTKVKGACTVFVPEETQLRPMQIVGGG
                     TGTELKAFTPVVRAVLAKLRPPSLQDNDGDLERLLKGIGCEPGTVEVPLSLLREVPRA
                     LREGEWTATAFLDGGRLVDLQPWDRTSRSFGAAVDLGTTTVALQLVDLGTGEMVAQVS
                     DYNRQLMYGEDVLARIMYCEENGGERLRRLAVDTIDHMLAQVCSSGEGCEGKGSRICA
                     DEVRAMVVAGNTTMVHLLLGLDPRSIRYEPYAPVTNLPPSTTAGELGVAINPRAAVH"
     gene            1082..>1349
                     /locus_tag="PLJ11_08565"
     CDS             1082..>1349
                     /locus_tag="PLJ11_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOO04758.1"
                     /translation="MEEHHPAGSVKMLAYALAITLVFALVEVAGGLISGSLALLGDAG
                     HMFTDVLALGLSLGAALMAGRVATERHTFGFLRAEVLVALVNGIA"
BASE COUNT          194 a          470 c          453 g          232 t
ORIGIN      
        1 ggtgcacggc cgccctgggg ttgatggcga cgcccagctc cccggcggtg gtgcttggcg
       61 ggaggttggt gacgggggcg tagggctcgt accggatgga cctcgggtcc aggccgagga
      121 gcaggtggac catggtggtg ttgcccgcca cgaccatcgc ccggacctcg tccgcgcaga
      181 tcctgctgcc cttcccctcg cacccctccc cggaggagca tacctgggcg agcatgtggt
      241 cgatggtatc cacggccaag cgcctgaggc gctccccgcc gttctcctcg cagtacatga
      301 ttcgggccag cacgtcctcc ccgtacatga gctgccgatt gtaatcggag acctgagcga
      361 ccatttcccc ggtgccgaga tcgacgagct gcagcgccac cgtggtcgtc cccaggtcca
      421 cggccgcccc gaaggagcgg gaggtgcggt cccagggctg gaggtccacc aaccgtccgc
      481 cgtccaggaa ggcggtggcg gtccattccc cctcccggag ggcccggggg acctccctca
      541 aaagggacaa gggcacctcc accgtccctg gctcgcagcc tatccccttc aggaggcgct
      601 ccaggtcacc gtcgttgtcc tgaagcgagg gggggcgcag ctttgcgagg accgcccgga
      661 ccacaggggt gaaggccttg agttccgtgc ctgtcccacc gcccacgatc tgcatcggcc
      721 tcagctgggt ctcctcgggc acgaacacgg tgcatgcgcc cttgaccttg gtcctgcagg
      781 ccaggcggta gccggtgctc cattcctcac cggtcacctt ggcgctgggc tcggattcga
      841 catccccttc cacgatgacc ttgcagcgcc cgcagacccc cttcccggcg caggcgctgt
      901 tgacctccac cccggccagg attgcggcct cgagcaggct ggtgccttcc gggacatcga
      961 tgctccggcc tttgggatgg aacgtcacct gggggctcat cccctgcgaa tccgttcacg
     1021 cgttatttaa ccttgaccgg acaggaaagg ctatcccgcg ggagatggct tgggggagcg
     1081 catggaggag caccaccctg cgggctcggt caagatgctt gcctacgccc tcgccatcac
     1141 cctcgtcttc gccctggtgg aggtggcggg cgggctcatc agcggaagcc tggccctgct
     1201 gggcgatgcc gggcacatgt tcaccgacgt cctggcgctg ggactgagcc tcggggcggc
     1261 gctgatggcg ggccgcgtgg ccaccgagag gcacaccttc gggttcctca gggcggaggt
     1321 cctagtggcc ctggtgaacg gcatcgcgc
//
LOCUS       DAOSYN010000333         1348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_72027_length_1348_cov_4.711524, whole genome shotgun sequence.
ACCESSION   DAOSYN010000333 DAOSYN010000000
VERSION     DAOSYN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72027_length_1348_cov_4.711524"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLJ11_08570"
     CDS             complement(<1..890)
                     /locus_tag="PLJ11_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO04759.1"
                     /translation="MRKTIRINELKAGGVVDDLFAVRFKKPVQQYKNGYSFQIWVSDA
                     GGDMALKYWGEKDERKVRALFDAIDKGSVVHVVGKVNEYNGALEIHVNPTSGDKVEPL
                     REGEYEVSDFVASTTEDVETMRTQLFAAIRKVKNEHLRRLLESFFGDEELVRRFCACP
                     ASITHHSNCIGGLLEHTLRVTKLCEFYAQQYAALDRDLLITGAILHDIGKLSEYTVTS
                     IIGATNEGRLVGHLVIGAGMVHDACRLIEGFPPELDLKVQHMVLSSHGTPEYGSPKGP
                     MFPEAQALALADLTDTRIED"
     gene            complement(942..>1348)
                     /locus_tag="PLJ11_08575"
     CDS             complement(942..>1348)
                     /locus_tag="PLJ11_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04760.1"
                     /translation="RNQPLYLRRSRNLAYCPRGRTHDLVSLGQDLRDLGLVNGVPHSY
                     VSWTKGENRQRVGYCSVIMRVVAISRILDRREVPSFVPSYVLYHELLHLENGLRFGAY
                     HDREFRERERMHPHHEEAERLLRKISASPQFR"
BASE COUNT          229 a          457 c          386 g          276 t
ORIGIN      
        1 atgtcctcga tcctcgtgtc cgtgaggtcc gccagggcca aggcctgcgc ctccgggaac
       61 ataggtccct tcggtgaacc gtactcgggg gtgccgtggc tggagagcac catgtgctgc
      121 accttcaggt cgagctcagg cgggaacccc tctatcagcc tgcacgcgtc gtggaccatc
      181 cccgccccga tcacgagatg gccgaccagg cgcccctcgt tggtcgcccc gatgatgctc
      241 gtcaccgtgt actcgctcag cttccctatg tcgtggagga tggccccggt gatcagcagg
      301 tcccggtcca gcgccgcgta ctgctgggcg tagaactcgc acagcttggt gacccggagc
      361 gtgtgctcca gcagcccccc tatgcaattg gagtgatggg ttatagaagc ggggcaggcg
      421 cagaacctcc ttacgagttc ctcgtcgccg aagaaggact ccagcagccg cctcagatgc
      481 tcgttcttga ccttccttat ggcggcgaag agctgcgtgc gcatcgtctc cacgtcctcc
      541 gtggtcgagg caacgaagtc ggagacctcg tactctccct cccgcagggg ttccaccttg
      601 tccccggagg tggggttgac gtggatctcc aacgccccgt tgtactcgtt gacctttccc
      661 acgacgtgga ccaccgaccc cttgtcgatg gcgtcgaaga gcgccctcac cttccgctcg
      721 tccttttccc cccagtactt cagggccatg tcccccccgg cgtcggacac ccatatctgg
      781 aaggaatagc cattcttgta ttgctggacg ggtttcttga agcgcaccgc gaacaggtcg
      841 tccaccaccc ctccggcctt cagttcgttg atccttatgg tcttgcgcat ctctctcccc
      901 tcctaacaaa ttccttcgac cataaagcaa ttggcatgcc ctcaccgaaa ctgcggggaa
      961 gcggagatct tgcgcagcaa ccgctccgcc tcctcatggt gggggtgcat gcgctccctc
     1021 tcccggaact cccggtcgtg ataggctccg aagcgcagcc cgttctcaag gtgcagcagc
     1081 tcgtggtaaa ggacgtaact agggacgaag gaggggacct ccctgcggtc tagtatgcgg
     1141 ctgatcgcca ccacccgcat gatcacactg cagtagccca ccctttgccg gttctcgccc
     1201 ttggtccagg agacgtagga gtgggggacg ccgttcacca gccccaggtc ccgcaggtcc
     1261 tggcccaatg agacgaggtc gtgcgtccgc ccacgagggc aatatgccag gttacggctc
     1321 ctcctcaggt acaggggctg gttcctca
//
LOCUS       DAOSYN010000334         1347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_72118_length_1347_cov_3.671053, whole genome shotgun sequence.
ACCESSION   DAOSYN010000334 DAOSYN010000000
VERSION     DAOSYN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72118_length_1347_cov_3.671053"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="argF"
                     /locus_tag="PLJ11_08580"
     CDS             complement(<1..885)
                     /gene="argF"
                     /locus_tag="PLJ11_08580"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOO04761.1"
                     /translation="MDEGEVRTMKRDLTTMLDLQDDILDILEMAEAMKKKRDMDAQPL
                     KGKVLAMIFEKSSTRTRVSFEVGMIELGGFAVTLNSKDLQMGRGETVADTARVLSRYV
                     DGIMYRAYEHSMVLELAKHATVPVINGLDNFEHPCQIMADLLTVKERFGRFKGIKMAY
                     MGDGNNVCNSLMLGCAMVGMDLACGCPPGYEPDPEVTKRAKAIAKANGCRLSIVESPK
                     EAAEGADIVYTDVWVSMGQEEEQRVREKVFRPYQVNAELLAGAHERAIVMHCLPAHRG
                     MEITDDVIDSERSVVLDQA"
     gene            complement(1013..>1347)
                     /locus_tag="PLJ11_08585"
     CDS             complement(1013..>1347)
                     /locus_tag="PLJ11_08585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO04762.1"
                     /translation="DLGPLDLGKLMLVMVVFVGFGEELVFRGLLQSSIASNFGKGVAV
                     LVSALLFASMHSGYHNVPYLCFVFGIGLALALAYERTGSLGLAALMHGLLNFFLFSFV
                     PFGYDLLP"
BASE COUNT          249 a          424 c          424 g          250 t
ORIGIN      
        1 ggcctggtcc aggacgacgg agcgctcgct gtcgatgacg tcgtccgtga tctccatgcc
       61 ccggtgggcg gggaggcagt gcattacgat cgcccgctca tgcgctcccg cgagcagctc
      121 cgcattcacc tggtagggcc tgaagacctt ctcgcgcacc cgctgctcct cctcctggcc
      181 catggacacc cagacgtcgg tgtacacaat gtcggcccct tccgcggcct ccttggggga
      241 ctccactatg ctgaggcggc agccgttggc cttggcgatg gccttcgccc tcttcgtgac
      301 ctcggggtcg ggctcgtatc cgggggggca accgcatgcc aggtccatgc cgaccatcgc
      361 gcacccgagc atgagcgagt tgcagacgtt gttgccatcc cccatgtacg ccatcttgat
      421 ccctttgaac cggccaaagc gctccttgac ggtgagcagg tcggccatga tctggcaggg
      481 atgctcgaaa ttgtcaaggc cattgatgac cggcacggtg gcgtgcttgg ccagctccag
      541 gaccatgctg tgctcgtacg ccctgtacat gatgccgtcg acgtagcggg agagcacccg
      601 ggcggtgtcc gccaccgtct ccccgcgccc catctgcagg tccttggagt tcagggtgac
      661 ggcgaagccg ccgagctcta tcatccccac ctcgaaggag acccgcgtac gggtggagga
      721 cttctcgaag atcatggcca gcaccttccc cttcagaggt tgggcgtcca tgtcccgttt
      781 tttcttcatg gcctcggcca tctccaatat gtcgaggatg tcgtcctgga ggtccagcat
      841 cgtggtcagg tcccgcttca tcgttcgcac ctctccttcg tccatcgcgg cgatgggacg
      901 cccatcgccc agggtgatgg aggattcgcc cttgtttaat aaaatgaatg atggggatgg
      961 aacattcggc cccgcttttg tcgggagagg ggtgcgatga cggaacgggc catcagggca
     1021 acaggtcata cccgaagggg acgaaggaga agaggaagaa gttcagcaaa ccgtgcatca
     1081 gggcggcgag gccgaggctg cccgtccgct cgtacgccag ggcgagcgcc agcccgatcc
     1141 cgaagacgaa gcagaggtag ggaacgttgt gatagccgga gtgcatggat gcgaacagga
     1201 gggcggatac gaggaccgcg acccctttcc cgaagttgga ggctatggag ctctgcaaca
     1261 gcccgcggaa gacaagctcc tccccgaagc cgacgaaaac gaccattacc agcatgagct
     1321 tgcccaggtc aaggggccca aggtccc
//
LOCUS       DAOSYN010000335         1337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_72925_length_1337_cov_1.957098, whole genome shotgun sequence.
ACCESSION   DAOSYN010000335 DAOSYN010000000
VERSION     DAOSYN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72925_length_1337_cov_1.957098"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>1337
                     /locus_tag="PLJ11_08590"
     CDS             62..>1337
                     /locus_tag="PLJ11_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04763.1"
                     /translation="MKGITSRTLLGTLLGWAIIFEGLFALSIAAQASIEGIGTILAST
                     FKLAAAQLTALGIFIGAMWALKMAFPNPRQTVLVWLFGLLTYLAMALVAMEELTMVLI
                     AGNVSVEGFGSVGKKWIVLIGAQLFAVAVLSLRLWRLRNVRPVNWLADTLGSLASAML
                     MLEGLTAIGIAGNATITGLGTVLERTMVYAGAGLFMLGALIFLVWTITSDPWAAPRLQ
                     KRIGQGWALPTMAVLGGLVAAAAVAVSTVAGSVAVVGAGSATKGIVVAGIAQLFALGL
                     VSPILWKLSLERLDRRALVQFIAAASMTMLAFEGVFAMGLAANTRIEGIGGILEGTFR
                     FAGAQLLVLSLVALTMWVLRDSPRVGRRAKLVISTLSLLSLTAIALEGLAATVLAGNL
                     RIDDLGSVRERYVLMGGAQMVLLASLALICQAR"
BASE COUNT          214 a          422 c          428 g          273 t
ORIGIN      
        1 agaatgattg cacaagttgt ataaatcagg agattggtaa ccccagagac cggggaagga
       61 gatgaagggc atcaccagcc ggacattgct cggaacgctt ttgggctggg ccatcatctt
      121 cgaggggctc tttgccctca gcatcgctgc ccaggcaagc atagagggca tcggcaccat
      181 cctcgcctcc acgttcaagc tcgccgccgc ccagctcacc gcccttggca tcttcattgg
      241 ggccatgtgg gccttgaaaa tggccttccc caacccgagg caaacagtgc tggtctggct
      301 gttcggcctc ctcacctatt tggccatggc cttggtggcc atggaggagc tgaccatggt
      361 cctcatcgcc gggaacgtga gcgtagaggg cttcgggagc gtcgggaaga aatggatcgt
      421 ccttataggg gcccagctct tcgccgtcgc cgtcctctcc ctgaggctat ggcgcctcag
      481 gaacgtccgc ccggtcaatt ggctcgcgga cactctgggc tcccttgcct ccgccatgct
      541 catgctggaa ggcctcaccg caatagggat cgcaggcaac gccaccatca ctggcctcgg
      601 aacggtcctg gaaaggacga tggtgtacgc cggtgcaggg ctcttcatgc tcggggccct
      661 gatctttctc gtttggacca tcaccagcga tccctgggcc gctccccgcc ttcaaaagcg
      721 catcggccag ggctgggccc tgcccaccat ggccgtgctc ggcggcctgg tcgccgccgc
      781 cgcggtcgcc gtctccactg tagcgggctc ggtggccgtg gtcggcgccg gcagcgctac
      841 caaaggcatt gttgtggcgg gcatcgccca gctgttcgcc ctggggctgg tctcgcccat
      901 cttatggaag cttagtctcg agaggctcga ccgccgggcc cttgttcaat tcatagcggc
      961 ggcctccatg accatgttgg ccttcgaggg cgtcttcgcg atgggccttg cggcgaacac
     1021 ccgcatcgag ggcatcggag ggatattgga ggggaccttc cggtttgccg gagcgcagct
     1081 gctcgtcctt tccttggtag ccttgacaat gtgggtcctt agggacagcc cgagggtggg
     1141 gagaagggcg aagctcgtca tctccacgct ctccctcctt tcgctgacgg cgatcgccct
     1201 cgagggattg gcggcaacgg tcctggccgg gaacctccgc atcgacgatc tcgggagcgt
     1261 cagggagcgg tacgtcctga tggggggagc gcaaatggtg ctcctggctt cgctggcgtt
     1321 gatttgccag gctcggc
//
LOCUS       DAOSYN010000336         1333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_73210_length_1333_cov_2.910016, whole genome shotgun sequence.
ACCESSION   DAOSYN010000336 DAOSYN010000000
VERSION     DAOSYN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73210_length_1333_cov_2.910016"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..>1333
                     /gene="xseA"
                     /locus_tag="PLJ11_08595"
     CDS             258..>1333
                     /gene="xseA"
                     /locus_tag="PLJ11_08595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOO04764.1"
                     /translation="MKVLSVLDLNTIAKQLIAECAELNDVWVAGEVSNLTKHSSGHYY
                     FTLKDSASEVRCTFFQTARARAEVELEENMRIIAFGSMDVYLKKGSYSFNVKKVQAQG
                     LGDLYLAYEALRRKLEAEGLFDPQRKRALPRYPRRVGVVTSPTGAAIRDILNISKRRF
                     PADILLAPALVQGEGAGESIVKGIALLNEAEVDVIIVGRGGGSLEDLWAFNEEVVARA
                     IYASKAPVVSAVGHETDFTISDYVADVRAPTPSAAAELVLPERNEEEKSLYHLLALAR
                     GNLRSVLDRMRQRMERTDRILSKGNIEHGIRDRQMELDILSDELERLMERYLLDRRNK
                     LESLQASLEGLNPINVLQRGYCLV"
BASE COUNT          287 a          366 c          442 g          238 t
ORIGIN      
        1 atcatcggga tgagtagcat gcgggtgctg gcatagggtt ccatcacatt cgacggtccc
       61 ccggaaagca ttatgatgcc cagcggtcct gaggttccga tggaagaagg aatggcatgg
      121 gctgaacggg gatcgggcac aggaggcttg ggcgccggcc cgctcgcgcc cttatcgacc
      181 tccgtggcgc cagagacgat ggccgcatcc gatccataca ccatctgtgg caggcaacat
      241 taccagaggg tttcgccatg aaggtccttt ccgttctcga cctcaacacc atcgccaaac
      301 aattgatcgc ggaatgcgcg gaactgaacg acgtctgggt ggctggggag gtatccaacc
      361 tcaccaagca cagttcgggc cattactact tcacgctcaa ggattcggcc agcgaggtcc
      421 gctgcacgtt cttccagacc gcccgggcga gggccgaggt ggagctggag gagaacatga
      481 ggatcattgc cttcggctcc atggacgtgt acctgaagaa agggagctac agcttcaacg
      541 tcaagaaggt tcaggcgcag ggcttgggcg atctgtacct cgcatacgag gcgctgcgca
      601 ggaaactgga ggcggagggg ctgttcgatc cgcagaggaa gagggcattg ccgcgctatc
      661 cgcggcgggt cggggtggtc acctctccca ccggggcggc catccgcgac atattgaaca
      721 tatccaagcg gcgctttcct gccgacatat tgctggcccc agcgttggtg cagggagaag
      781 gggcggggga aagcatagtc aaaggcatag cgttgctcaa cgaggccgag gtggacgtga
      841 taatcgtcgg ccggggcggg gggtccctgg aggacctgtg ggccttcaac gaggaggtag
      901 tggccagggc gatatacgca tcgaaggccc ccgtggtctc cgcggtaggc catgagacgg
      961 actttaccat aagcgattac gtggccgacg tgcgtgcccc gaccccctcg gccgcggccg
     1021 agcttgtcct tcccgagcga aatgaagagg aaaagagcct gtaccatctt ctagccctgg
     1081 ccaggggcaa cctgcggtcg gtgctggata ggatgaggca gaggatggaa cgcaccgacc
     1141 gcatcctttc caaggggaac atagagcatg ggatcaggga caggcagatg gagcttgaca
     1201 tcctctccga cgaactggaa aggctgatgg agcggtacct gttggacagg cgcaacaaac
     1261 tggaatccct gcaggcgtcg ttggaaggcc taaatcccat taacgtgctc cagcgcggtt
     1321 attgcctggt gca
//
LOCUS       DAOSYN010000337         1326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_73747_length_1326_cov_3.240755, whole genome shotgun sequence.
ACCESSION   DAOSYN010000337 DAOSYN010000000
VERSION     DAOSYN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73747_length_1326_cov_3.240755"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLJ11_08600"
     CDS             complement(<1..250)
                     /locus_tag="PLJ11_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04765.1"
                     /translation="MLELLARSGTARRCLWTVEGAKAETPGAFIVNGGSAGLGLRLTK
                     EGTVLELDGQAVLGIKGDGLLPPLRSHAGMRERCLPPTI"
     gene            complement(299..658)
                     /locus_tag="PLJ11_08605"
     CDS             complement(299..658)
                     /locus_tag="PLJ11_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOO04766.1"
                     /translation="MTDSLLILVTKPPYGFEEAFSGLRAGLGALASGLVPTVNILMVG
                     EGTLNAVAAQRPEAVRMPSNVEAVNDMLDFEAKVYVVREDLLRLVGDVPLLEGVETID
                     WERARQVIEGHGMVTTF"
     gene            complement(664..1020)
                     /locus_tag="PLJ11_08610"
     CDS             complement(664..1020)
                     /locus_tag="PLJ11_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOO04767.1"
                     /translation="MRTMVIQIRSGTMMNMDTNIAVKLARAALDKGYGVRMFGYGEGV
                     TLIKKGQDPKRFPNVGNETKELVEKGLEVVVCETCCKARGIARGEEIEGTKIGSLTND
                     LSRFVAEGDRMVTLAR"
     gene            complement(1030..>1326)
                     /gene="tusB"
                     /locus_tag="PLJ11_08615"
     CDS             complement(1030..>1326)
                     /gene="tusB"
                     /locus_tag="PLJ11_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HOO04768.1"
                     /translation="RSKLFITLKSPHDLGDLEMVRALAKGSRSGLLLLEDAVIFAANA
                     ELGKELLGAVQDVYVIRDDWEARGLPLANPGFKVVDYPEAVDLIMERYEQTITV"
BASE COUNT          218 a          480 c          384 g          244 t
ORIGIN      
        1 ctatggtcgg tggaaggcac ctttccctca tcccggcatg gcttcgaagc ggggggagga
       61 ggccgtcgcc cttgatcccc agaacggcct ggccgtccag ctccagcacc gtcccctcct
      121 tggtgaggcg caggccgagg ccggccgacc ctccgttcac gatgaacgcg ccgggggtct
      181 cggccttggc cccttccacg gtccagaggc agcgccgggc ggtgcccgac ctggccagaa
      241 gctccaacat ggaactggga acggcatccc taataaaaaa gcatgggcgg gaaggggttt
      301 agaaggtggt gaccatgccg tgcccctcga tgacctggcg cgccctctcc cagtcgatgg
      361 tctccacccc ctccaggagc ggaacgtccc cgaccaggcg gaggaggtcc tccctcacca
      421 cgtacacctt ggcctcgaag tccagcatgt cgttgaccgc ctccacgttc gagggcatgc
      481 gcaccgcctc aggccgctgg gccgccacgg cgttcagggt cccctcgccc accatcagga
      541 tgttgacggt cggcacgagc cccgaggcca gggcgccaag cccggcgcgg agcccggaga
      601 aggcctcctc gaagccgtag gggggcttcg tgaccaaaat aagcagactg tccgtcatcc
      661 ttgtcacctc gccagggtca ccatgcggtc gccctcggca acgaacctgg acaggtcgtt
      721 ggtcaggctt ccgatcttgg tgccttcgat ctcctctccc cgggcgatcc cccgggcctt
      781 gcagcaggtc tcgcagacga ccacctctag ccccttctcg accagctcct tggtctcatt
      841 gcccacgttc gggaagcgct tggggtcctg ccccttcttg atcagggtga ccccctcccc
      901 gtagccgaac atgcgcacgc cgtacccctt gtccaaggcg gccctggcca gcttgaccgc
      961 gatgttggtg tccatgttca tcatggtgcc cgacctgatc tgaatcacca tcgttctcat
     1021 ccttccacct cagaccgtta tcgtctgctc gtacctctcc atgatcagat cgaccgcctc
     1081 gggatagtcc acgaccttga acccggggtt ggccaagggc agccccctgg cctcccagtc
     1141 gtccctgatc acgtagacgt cctggaccgc gcccaagagc tcctttccca gttcggcgtt
     1201 ggccgcgaag atgaccgcgt cctcgaggag gagcaacccg ctcctggacc ccttggctag
     1261 agcccgcacc atctccagat cgccgaggtc gtgcggggac ttcagcgtta tgaacagctt
     1321 ggacct
//
LOCUS       DAOSYN010000338         1320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_74258_length_1320_cov_1.802372, whole genome shotgun sequence.
ACCESSION   DAOSYN010000338 DAOSYN010000000
VERSION     DAOSYN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74258_length_1320_cov_1.802372"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLJ11_08620"
     CDS             <1..503
                     /locus_tag="PLJ11_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HOO04769.1"
                     /translation="SYGLRFYRNALLKFYGHMTSSPRTGLRGRPRVPALVPDNGLQYA
                     QVIKRHRNGHLVEVEKRQIFGVDAGSASISTTFIERCNLTILQENKRLTRKTLTFSKR
                     QYRLDAHMTLYFANYNLCRPHGSLRHPDASGKMRKWTPMRELGISDRNWSLKELLTFP
                     YHKTSV"
     gene            674..1123
                     /locus_tag="PLJ11_08625"
     CDS             674..1123
                     /locus_tag="PLJ11_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO04770.1"
                     /translation="MAVEKEIKDINLGLRSEFDARHDAQLIFEIPGIGWYGALTILAE
                     IGKIKRFPDAQHLAAYAGLVPSVHQSANGCYYGHISKQGLKYMRWILVEAVHIHVRTA
                     SDSAITKFFNRKQKRSNRNIATIAAARKMAVAIFHMLDKQEHFRITG"
BASE COUNT          332 a          343 c          357 g          288 t
ORIGIN      
        1 gcagttatgg cctgaggttc tacaggaacg ccttactgaa gttttacggt cacatgacct
       61 cctccccgag aactggcctg cgcggcaggc caagggttcc tgcgttggtc ccggataacg
      121 gtctccaata cgcccaggtc atcaaacgtc atcggaacgg tcatctggtc gaggtggaga
      181 agaggcagat attcggagtg gatgctggct cggccagcat ctccacgacg ttcatcgagc
      241 ggtgcaacct caccatactt caggagaaca aacggttgac gaggaaaaca ctgacgttct
      301 ctaaacgcca gtatcgtttg gatgcgcata tgacactgta tttcgccaat tacaatctct
      361 gccgaccgca tggctcattg aggcatccag acgcttcagg aaagatgaga aaatggacgc
      421 cgatgaggga gcttggcatc agcgatcgga attggagcct gaaagagcta ttgacctttc
      481 cttatcataa aacatcagta taatacaggg tcagtatcat ctcttctttc tttaaaattt
      541 tagtacttac gtatatgccc tatttcagat cttggctacc gtcgttttag caacggtttg
      601 cacttatgat tgaacgttct ttggtttatt aggaacagct aagtcgcggt tctagccacc
      661 ctgccctggg aacatggcgg tggaaaagga gataaaggac atcaacctcg gtctgaggtc
      721 agagttcgat gccagacatg acgctcagct catcttcgag atacctggaa taggatggta
      781 cggggcactg acgatactgg cggagatcgg gaaaatcaag cggttccctg acgcgcagca
      841 tctggccgcc tacgccggac tggtgccttc agtgcaccag tccgccaatg gctgctacta
      901 cggccatatc agcaagcagg gcttgaagta catgcgctgg atattggtgg aggcggtgca
      961 catccatgtg cgcaccgcct ccgatagcgc catcaccaag ttcttcaacc ggaagcaaaa
     1021 gcggtcgaat aggaacatcg ccaccatcgc cgccgcaagg aagatggcgg tcgccatctt
     1081 ccacatgctg gacaagcagg agcacttcag gatcacgggc tagacctcgg gtcttttctt
     1141 gcgtccccga acgccgaaaa aagaatgagg cgggcccgtg acaaggggct gagtgccata
     1201 gtgcgtcctc gaacgcggat agaaacttgt ccgaccgcct cgaattgacc ggcagggacg
     1261 aaaaataaaa gctacgtcga ggggatcctc acttgcgcga gggtcccttc aacatagaag
//
LOCUS       DAOSYN010000339         1316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_74535_length_1316_cov_2.618557, whole genome shotgun sequence.
ACCESSION   DAOSYN010000339 DAOSYN010000000
VERSION     DAOSYN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74535_length_1316_cov_2.618557"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLJ11_08630"
     CDS             complement(<1..343)
                     /locus_tag="PLJ11_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOO04771.1"
                     /translation="MTRVAVIAGDGIGPEVVGATMRLLGMMDLGLEFVEASMGLECFK
                     RTGEYLPRETLRVLEGSKAALFGAITSPVAPDPSYSSPLLELRRRFDLYANVRPCFPL
                     LPELRVTDLNAV"
     gene            complement(340..1272)
                     /locus_tag="PLJ11_08635"
     CDS             complement(340..1272)
                     /locus_tag="PLJ11_08635"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOO04772.1"
                     /translation="MEGREVRDIKVKEGMTVDEFVRAMSDSGGFTAKKLADAVDIVER
                     MVRDEGCLTFLSFPACIMATGTRGVIVELVKRKLVDVVITTCGTLDHDLARTWKRYYH
                     GDFAMDDAELREQGINRLGNVLVPDESYGLVLEERLVPMFEEILQGRDSISTREVIDQ
                     VGARLDSEDSLLYWAHRNGVPIFVPGITDGSFGSQLWMYWQTHRDLKVDLFEDEQELA
                     DLVFRAERSGALIVGGGISKHHVIWWNQFRGGLDYAVYMTTAVEYDGSLSGAQVREAI
                     SWGKVREDAWQVNVEGDATITLPILVAALVERSR"
BASE COUNT          235 a          464 c          385 g          232 t
ORIGIN      
        1 tcacagcgtt caggtccgtg accctgagtt cggggagaag ggggaagcag gggcggacat
       61 tggcgtacag gtcgaagcgc ctgcgcagct ccagcagggg ggagctgtag gaggggtcgg
      121 gggcgacagg ggaggtaata gctccgaaca gcgcggcctt ggagccctcg agaaccctta
      181 gcgtctcccg cgggaggtac tcgcccgtgc gcttgaagca ctccaggccc atggaggcct
      241 cgacgaattc cagcccgagg tccatcattc ccaataggcg catggtggcc ccgaccacct
      301 cggggccgat gccgtccccc gcgatcaccg ctacccgggt catcgcgatc gctcgacgag
      361 cgccgccacc aggatcggca gggtaatggt ggcgtccccc tccacgttca cctgccacgc
      421 atcctccctc accttgcccc aggagatggc ctcgcgcacc tgggccccgc tcagggaacc
      481 gtcgtactcg acggcggtgg tcatgtacac cgcgtagtcc agaccgcccc ggaactggtt
      541 ccaccagatg acatggtgct tggaaatccc cccgcccact atcagggcgc cgctccgctc
      601 cgccctgaag acaaggtccg ccagctcctg ctcgtcctcg aacaggtcca ccttcaggtc
      661 cctgtgggtc tgccagtaca tccatagctg gctcccgaag gacccgtccg ttatcccggg
      721 cacgaagatc ggaacaccgt tccggtgcgc ccagtataga agggagtcct cgctgtccag
      781 gcgggcgccc acctggtcta tgacctcccg ggtggatatg gagtcccgcc cctgcaggat
      841 ctcctcgaac atcgggacca gccgctcctc gagcacaagg ccgtagctct catcggggac
      901 aagcacgttc cccaggcggt tgatgccctg ctcccgcagc tccgcatcgt ccatggcgaa
      961 gtccccatgg tagtagcgct tccacgtgcg ggccaggtca tggtccaagg tgccacacgt
     1021 ggttatgacc acgtcgacca gcttgcgctt gaccagctcc actattacgc cccgggtccc
     1081 ggtggccatg atgcaggccg ggaacgagag gaaggtcagg cacccttcgt cccggaccat
     1141 cctctcaacg atgtcaacgg catcggccag cttcttggcc gtgaacccgc cggaatcgga
     1201 catggcccgg acgaactcgt ccactgtcat gccctccttg accttgatgt cgcgtacctc
     1261 tctcccttcc atagctatcg cccatactat cccgagcccg tagaaatacg ttgcct
//
LOCUS       DAOSYN010000340         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_74993_length_1310_cov_2.941036, whole genome shotgun sequence.
ACCESSION   DAOSYN010000340 DAOSYN010000000
VERSION     DAOSYN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74993_length_1310_cov_2.941036"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PLJ11_08640"
     CDS             complement(<1..1114)
                     /locus_tag="PLJ11_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOO04773.1"
                     /translation="MRELFEPASVAIIGASPDRTKVGNIILGNMIESGFQGPLYPVNP
                     RYEEIMGLRCYPRVTDVPGPVEMAVIVVPAKAVLQVTEECGQKGVGAIVVISAGFKEV
                     GLEGAVLERRLGELAHRYGMRVLGPNCLGLVNTHHRMNATFAREGPREGNIAISSQSG
                     AICVVILDWAANINIGFSKFVSVGNKLDVDEGHLLEYLREDPFTKVIGMYIEGTDRGR
                     EFLRQAELTTRVKPVIALKAGRTSSGAKAASSHTGAMSGSDRVYDAAMRQAGVVRVGN
                     IEEMFDLLQAFSTMPLPQGEGVAIVTNAGGLGVMAADACSDNGLTLASFEPATVERLR
                     EGLPPAASLYNPIDVVGDADAARYDHAIRTVMADPNV"
BASE COUNT          214 a          447 c          414 g          235 t
ORIGIN      
        1 ccacgttggg gtcggccatc accgtgcgga tggcgtggtc gtagcgggcg gcgtcggcgt
       61 cgcccaccac gtcgatggga ttgtagaggc tggccgccgg tggaagcccc tccctgagcc
      121 tttcaacggt ggccggctcg aacgatgcca gggtgagacc gttgtccgag caggcgtccg
      181 cggccatgac ccctaggccg cccgcgttgg tgacaatagc cacgccctcc ccctggggca
      241 ggggcatggt cgagaaggcc tgtagcaggt cgaacatctc ctcgatgttc cccaccctca
      301 ccaccccggc ctgcctcatg gccgcgtcgt agaccctgtc gctcccggac atggcgccgg
      361 tgtgggacga ggcggccttg gcgccgctgc tggtgcgccc cgccttgagg gctatgaccg
      421 gcttcaccct cgtggtgagc tccgcctggc gcaggaactc ccggccgcgg tcggtgccct
      481 cgatgtacat gccgatcacc ttggtgaagg ggtcctcgcg gaggtattcc aaaagatgcc
      541 cctcatccac gtccagcttg ttcccgacgc tgacgaattt ggagaacccg atgttgatgt
      601 tcgccgccca gtccaggatg acgacgcata tcgccccgga ctgggaggat atggcgatgt
      661 ttccctcccg ggggccttcc ctggcaaagg tggcgttcat gcggtgatgc gtgttcacca
      721 gcccgagaca gttcggcccg agcacccgca ttccgtaccg gtgcgccagc tcgcccagcc
      781 tccgttcgag cacggccccc tccagcccca cctccttgaa gccggcgctg atgaccacga
      841 tggccccaac gcccttctgg ccgcattcct ccgtcacctg caggaccgcc ttggccggga
      901 cgacgatgac ggccatctcc accggccctg gcacatccgt cacgcggggg tagcacctca
      961 agcccatgat ctcctcgtag cgcgggttca ccgggtacag ggggccctga aaccccgatt
     1021 cgatcatgtt cccaaggatg atgttcccca ccttggtccg gtccggggag gcgccgatga
     1081 tcgccacgct cgcaggttcg aacagctcac gcatctgggg gagtaatggc caggtgcggg
     1141 acatatcctt tgcggtcgga cctcgggacc ggggaaaata tattaaggac cggggacgtg
     1201 atgccctccg ccatcagtac agatgagggg gaacggtgat ccctccaata gcctgatggc
     1261 gcacagtgcg ccgataggtc cctgcaggaa cggcgatgcg acatcgcgtc
//
LOCUS       DAOSYN010000341         1307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_75244_length_1307_cov_2.035144, whole genome shotgun sequence.
ACCESSION   DAOSYN010000341 DAOSYN010000000
VERSION     DAOSYN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75244_length_1307_cov_2.035144"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLJ11_08645"
     CDS             <1..283
                     /locus_tag="PLJ11_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04774.1"
                     /translation="NIVLGNKQEVKNMKTVKIQNKGPTMSNRKYHDYGCPTWWARVGN
                     GDDAIFLPIPKVRGDYPLNCNVQVNDDVTEIYIGVGPKGKYGVRETIKC"
     gene            504..863
                     /locus_tag="PLJ11_08650"
     CDS             504..863
                     /locus_tag="PLJ11_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04775.1"
                     /translation="MEQNFEILKSLVDEGRVIHGCHGGIGYDIALIEMKDDHVECWEE
                     MIGDFSILLEDFDGCQSEYSIENEIWHAFIEKLKAMEERADGRLMSCARCGHHWFTRV
                     SSPKKCPVCQARLGQVE"
     gene            892..1107
                     /locus_tag="PLJ11_08655"
     CDS             892..1107
                     /locus_tag="PLJ11_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04776.1"
                     /translation="MAKIKRGAIEQILKRINSTDPQYEWLRGSYDTLAQIIAEIEQIR
                     PGIDREVVDVNAPTWNQRLMEIMPTHD"
BASE COUNT          448 a          209 c          333 g          317 t
ORIGIN      
        1 caatatagta ttaggaaata aacaggaggt aaaaaacatg aagaccgtaa aaatacagaa
       61 caagggaccg acaatgagca accgaaaata ccacgactat ggatgcccta cttggtgggc
      121 ccgggtaggc aatggggatg atgccatatt cctcccgatc cccaaggttc ggggggatta
      181 cccgctcaac tgtaatgtac aggtcaatga tgatgtaacc gagatatata ttggagtggg
      241 ccccaagggc aagtatggag ttcgtgaaac gatcaagtgc tgaaggaggg ctaaaaaatg
      301 gaaaaaatac aggtacgaat aaatcataaa gtggttaatg aatttgaaag tatgatcgaa
      361 tttatgagat ttttagatcg tcataattat catactgagg acgatcttgg aggccagata
      421 aagcagacat tcattacaat ggatgacaat ttcggtaggc cgcatctagt aggactagct
      481 gaaatagtca aggaggagta aaaatggaac agaattttga aatattgaaa agtctggttg
      541 atgaaggccg tgtgatccat ggatgccatg gaggcatagg ctatgatatc gcgctcatcg
      601 aaatgaagga tgatcatgtg gaatgctggg aggaaatgat cggagacttc agtattctgc
      661 tcgaagattt cgatgggtgc caaagtgaat acagcataga aaatgaaata tggcatgcat
      721 tcattgaaaa gctaaaggct atggaagagc gagcagatgg gcgattaatg tcttgcgctc
      781 gatgcgggca tcattggttc actagggtat caagtcctaa aaaatgccct gtatgtcaag
      841 ccagattagg acaggtggag taacatggat gaattatacc atctagtaaa gatggcgaaa
      901 ataaagcgtg gagcaattga acagatatta aagcgaatca attcaactga tccgcaatat
      961 gaatggctga gaggtagcta tgacacgctc gcccagatta tcgccgaaat tgaacagatt
     1021 cgacccggaa tagatcgtga agtggtcgat gtaaatgctc cgacatggaa tcagcgcctg
     1081 atggaaataa tgccgactca tgattgaggt gagaaaatga cagcaggact tgatgaatcc
     1141 attatgataa tggattcact aaatgaggaa taatatgata gataattgca tctataatca
     1201 ctggtggatt gcgaacgaat ctaaaaacca caaattctgt ttttttttgt ggtgaaaaca
     1261 tcgaacgttt ccacgatgtg aagatgggag atcgtgatta tttcata
//
LOCUS       DAOSYN010000342         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_75692_length_1301_cov_2.829856, whole genome shotgun sequence.
ACCESSION   DAOSYN010000342 DAOSYN010000000
VERSION     DAOSYN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75692_length_1301_cov_2.829856"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLJ11_08660"
     CDS             <1..1205
                     /locus_tag="PLJ11_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04777.1"
                     /translation="EDHLDQVQQAQAAKESELKEREAKVAPREEAARQMEMELAALGA
                     ELRTREEEMHRLQVDLERRDRDVLEREEQVRELFQQAMEHDAKLREVTRKVSAMEERM
                     HDEVDQVAQALEAVNARREEMLAKIKVMEEQADALANGKRVTMEEQKRLVDWERSLAE
                     REEALSAREAVLMRREGAPLALNEHFEGGEREERIAQSLVGKVEDVPEPIVPAVGPEA
                     PEAKEGSKEAAQETKEAPPVKAPTDVASAPKEEAASPRPRPRKGAAPAPEEAPVPKGE
                     PSTAAPEPQAEEAPIAGTPASQPMPDITCPSCSTINDGSAKRCYACGQRLDGDSIKAF
                     EAKRAARQAEEARKEAERKASTERKDTEHMAEEKGPQAEGAKEGQSPQPPTEEKKPVS
                     IRKIIKRK"
BASE COUNT          294 a          369 c          490 g          148 t
ORIGIN      
        1 aggaggacca tctcgaccag gtgcagcagg cccaggcggc caaggagtcg gagctcaagg
       61 agcgcgaggc caaggtggcc ccgcgcgagg aggctgcaag gcaaatggag atggagctgg
      121 ccgccctcgg cgccgagctc cgcacccgcg aggaggagat gcaccgcctt caagtggacc
      181 tcgagaggag ggaccgggac gtgctggagc gggaggagca ggtcagggaa ttgttccagc
      241 aggccatgga gcatgacgcc aagctgaggg aggtgaccag gaaggtgtcc gccatggagg
      301 agcggatgca tgacgaggtc gaccaggtcg cgcaggccct ggaggcggtg aacgcccgcc
      361 gggaggaaat gctggccaag atcaaggtca tggaggagca ggccgacgcc ctggccaacg
      421 ggaagcgggt caccatggag gagcagaagc gcctggtgga ctgggagcgc tccctggccg
      481 agagggagga ggcgctgtcg gcaagggagg ccgtgctgat gaggagggag ggggcgccgc
      541 tcgcgctcaa cgagcatttc gagggcggcg agagggagga gaggatagcc cagtccctcg
      601 tcggcaaggt ggaggacgtc cccgagccga tcgttccggc agtggggccg gaggcccccg
      661 aggcaaagga ggggtccaaa gaggccgccc aggaaacgaa ggaggctccc ccggtgaagg
      721 cgcctacgga cgttgcttcc gccccgaagg aggaggccgc atcaccaagg ccgaggccga
      781 ggaagggggc cgctcccgct ccggaggagg cccctgtgcc aaagggcgaa ccaagcacgg
      841 ccgcgcccga gccgcaggcg gaggaggcgc ctatagccgg gacgcccgcc tcccagccaa
      901 tgcctgacat cacctgtccc agctgcagca cgatcaacga cggctcggcg aagaggtgct
      961 acgcctgcgg ccaaaggctg gacggggatt cgataaaggc gttcgaggcc aagagggcgg
     1021 cgcgccaggc cgaagaggcc cgcaaggagg cggagcgcaa ggcctccacg gaaaggaagg
     1081 atacggaaca tatggccgag gagaaggggc cccaggccga aggggcgaag gaggggcaat
     1141 cgcctcagcc tcccacggag gaaaagaagc ccgtctcgat ccggaagata ataaagcgga
     1201 agtgacctac ccccttccgg ttttccctta gccagcgctt tcacgaaagc attcaggtag
     1261 acatatttat ttataatcag aacattctat tttacctggc g
//
LOCUS       DAOSYN010000343         1297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_76043_length_1297_cov_1.994364, whole genome shotgun sequence.
ACCESSION   DAOSYN010000343 DAOSYN010000000
VERSION     DAOSYN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76043_length_1297_cov_1.994364"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..902)
                     /locus_tag="PLJ11_08665"
     CDS             complement(270..902)
                     /locus_tag="PLJ11_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOO04778.1"
                     /translation="MVGLGQIPYLEFFVEEPSMSIALSNLLPKVFIDKDVRWDFREFQ
                     GKMDLLKKLPDRLKGRAKCMQPCHKIVVIVDEDREGCHMLKGKLESIAAQQGLLTKTT
                     ASRGDYQVLNRIAVEELEAWFFGDPSAVIAAYPKVSPKAFSKAEYRVPDNIKGGTWER
                     LDKILQKAGYYSTGMIKKEVADRISLHMDVERNTSESFKTFVRGLRDAVR"
     gene            complement(912..>1297)
                     /locus_tag="PLJ11_08670"
     CDS             complement(912..>1297)
                     /locus_tag="PLJ11_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO04779.1"
                     /translation="YASDGTLKMLAYMTLMNDPSPPHLVGVEEPENFIHPSLLGDLLG
                     EFRKASQRTQLLITTHSPFIVNEAKPEEVYVLFRDDKGHTNARRAKDMPGIRELVKAG
                     GKLGDLWMERFFDVGYPSSPSNGRG"
BASE COUNT          291 a          349 c          311 g          346 t
ORIGIN      
        1 tttcgtcgat atgaacggtt catagatggc ttcgcatggt gctttttatc gactcgttta
       61 cttggtgacc gcccatgata gagacgggaa cagaagccga tacgataagc ggggatggat
      121 tttgaaagcg gaatgtaaat gtcgttccga tatggtgttg aaatggaacc gatccgaata
      181 tcagtatctc ccatccttcg tgacatcgac atcgttgtca attgcggacg ttgggaaggt
      241 tgcggcggat agcggagatc gggaccttgt tatcgtactg catccctcag cccccgaacg
      301 aatgtcttga aactttcgga tgtgttcctt tcaacgtcca tatggagaga gatcctatct
      361 gcgacctctt tcttgatcat cccggtagaa taatacccag ctttctgaag tatcttatcc
      421 aggcgttccc acgtgccacc cttgatgttg tccggaaccc tgtattcggc ctttgagaac
      481 gctttcggcg acaccttcgg atacgccgcg atcacagcgg acgggtcccc aaagaaccaa
      541 gcctccaatt cttccacggc gatcctgttg agaacctgat aatcgcccct tgaggctgtg
      601 gtcttggtca acaatccctg ttgggccgct atagattcca gtttcccctt gagcatatga
      661 cagccctccc ggtcctcgtc gacaataacg acgatcttgt gacagggctg catacatttg
      721 gcccttccct tcaatcgatc cggtagtttt ttcaaaaggt ccatctttcc ttggaattca
      781 cgaaaatccc agcggacatc cttatcaatg aatacctttg gcaggagatt tgatagagcg
      841 atgctcatcg agggctcctc aacgaagaac tctaggtagg ggatctgtcc taatccgacc
      901 attgcattcc cttaccctct tccattcgat ggactggatg gataccctac gtcaaagaac
      961 cgctccatcc atagatcgcc caacttacct cctgccttga ccagctcacg tatgccaggc
     1021 atgtccttgg cccttcgtgc attggtgtgc cctttatcgt cgcgaaacag cacgtagacc
     1081 tcttcgggct tggcctcgtt gactatgaac ggggaatggg tggtgatcaa caactgagtc
     1141 ctctgagatg cctttcggaa ctccccaagg aggtctccta aaagggacgg atggatgaag
     1201 ttctcaggct cttcgacccc tacaagatgc ggcggtgatg gatcgttcat cagcgtcatg
     1261 tatgccagca tcttcaatgt tccatcggac gcgtatt
//
LOCUS       DAOSYN010000344         1295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_76177_length_1295_cov_2.387903, whole genome shotgun sequence.
ACCESSION   DAOSYN010000344 DAOSYN010000000
VERSION     DAOSYN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76177_length_1295_cov_2.387903"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..>1295)
                     /locus_tag="PLJ11_08675"
     CDS             complement(137..>1295)
                     /locus_tag="PLJ11_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase GatDE subunit E"
                     /protein_id="HOO04780.1"
                     /translation="QDLGLLPTYVRTEMERQHALIEIKRLLRERGATPVPLAVRDVTA
                     SLRDSRSKVLRSALSKGGKVLCAPLPLFAGALRSADGRLRLGAEMAQHARSRGVAGIF
                     HSDELPGYGITAEEVQALRAELRLGERDAFVLCADMEDRAGPALLAALARANQALVGV
                     PEETRDPLPDGTSVYSRPLPGAGRMYPETDVRPILIEPERLAGIRATLPELPEARIAR
                     LVGEYAINEQQARQLVRDGHDGLFEEVGRGKGMAAVAARTFLSTYPELQKEGVDLEAL
                     SDAKVREAFAALSAGRFAKEALPSVLREMAKGADAEGAMAALGLEAIDQRDAVRVLDA
                     IIAERAEFVREKGEGAVGPLMGAAMKELRGKVDGKAAAELLRERVRAFLEG"
BASE COUNT          160 a          481 c          449 g          205 t
ORIGIN      
        1 gcaccgggcg gcagggcgct cgtgctcgtc tccagcgatg cggagccggg ggcggtggcg
       61 cgcgcgatgg agggctggaa cgtccggaca ttgacctccc ggaccctctt ctttgaggag
      121 ctgagggcgc tggagctcac ccttcgagga acgccctgac cctttccctg agcagctcgg
      181 cggcggcctt gccgtccacc ttgccccgca gctccttcat cgccgccccc atgagggggc
      241 cgaccgcgcc ctctcccttc tccctcacga actcggcgcg ctcggcgatg atcgcgtcga
      301 ggacccggac cgcgtccctc tggtcgatgg cctccaaccc gagggccgcc atggccccct
      361 ccgcgtccgc gcccttggcc atctccctta ggacggaggg cagggcctcc ttggcgaacc
      421 ggcccgcgga gagggcggcg aaggcctccc tgaccttggc gtcggacagg gcctccaggt
      481 ccaccccctc cttctgcagc tccgggtagg tgctcaggaa ggtgcgggcg gctacggcgg
      541 ccatcccctt gccccggcca acctcctcga acagcccgtc gtgcccgtcc ctgacgagct
      601 ggcgcgcctg ctgctcgttg atggcgtatt cgcccaccaa ccgggcgatg cgcgcctctg
      661 gaagctccgg aagggtggcc cttatgcctg ctagtctctc gggctcgatc aggatcggcc
      721 tgacgtccgt ctccgggtac atgcgcccgg cgcctggcag ggggcggctg tacacggagg
      781 tgccgtccgg cagggggtcc ctcgtctcct ccggaacccc caccagcgcc tggttggccc
      841 gggcgagggc ggccaggagg gcgggccccg ccctgtcctc catgtcggcg cataggacga
      901 acgcgtcccg ttcccccagc cgaagctcgg cccgtagggc ctggacctcc tcggcggtga
      961 tgccgtaccc gggcagctcg tcggaatgga agatgccggc gacgccgcgg gagcgggcgt
     1021 gctgggccat ctccgccccc agcctcagcc ttccgtccgc gctgcgcagg gcgccggcga
     1081 agagcgggag gggggcgcag aggaccttcc ctcccttgga gagggcggaa cggaggacct
     1141 tggacctgct gtccctcagc gatgcggtga cgtccctgac ggccaggggg acgggggtcg
     1201 cccccctctc ccgcagcagt cgcttgatct ctatgagcgc gtgctgccgc tccatctcgg
     1261 tgcggacata ggtgggcagc aggcccaggt cctgg
//
LOCUS       DAOSYN010000345         1294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_76242_length_1294_cov_3.271186, whole genome shotgun sequence.
ACCESSION   DAOSYN010000345 DAOSYN010000000
VERSION     DAOSYN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76242_length_1294_cov_3.271186"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLJ11_08680"
     CDS             complement(<1..186)
                     /locus_tag="PLJ11_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04781.1"
                     /translation="MKWRSHISIGKAIADRLELPPGERKAFLEGLVEPDRHKERIGGP
                     RRSYRVGHHHPSHRTFML"
     gene            667..>1294
                     /locus_tag="PLJ11_08685"
     CDS             667..>1294
                     /locus_tag="PLJ11_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04782.1"
                     /translation="MLETIAGAGDALLQILLLIFSYAFLGGTIKYIDQAYDKGIGSLK
                     AAKPLAVVSGILMGVVMVIDGGFSTAFFLAMLISLVLTKKIDNQSFLVGTISGVATFL
                     VGGSVWGMDILPLPLVVFIIAGIVDELADALAHDRNLGRAAGLLLHYRPFSDIALVAL
                     IVATPFDWTYLAPYFAFTLSYLLMGMREEGQVGGSVRGIIRKAGDLRPW"
BASE COUNT          254 a          358 c          369 g          313 t
ORIGIN      
        1 cagcatgaac gttcgatggc tgggatgatg atggcctacc cgatatgaac gcctcgggcc
       61 ccctatgcgc tccttatgcc ggtcgggctc taccagtccc tccaagaagg ccttccgctc
      121 ccccggtggg agctccagcc gatcggctat ggccttgccg atggagatgt gggagcgcca
      181 tttcatcggg tgcgatcgat gggctgcccc ctatttaatt tttgaaggag gatggaaagg
      241 ccgccagggc catttccctg gcaaaaaaag aaaaagaacg gtgaggttcg aagtccttaa
      301 gccttcttct tgggctccgc cttcttctcc ggtttctttg ccatcggtaa accacctccg
      361 ttccccaatg ggactgaggg ttttaatgta tctgaaatat ataagtactt ttgcagttcc
      421 agacatcaat tatcaccatt taaatattaa ttgtctgaaa tcaaactttt acgccgttaa
      481 catgctacgt cgaggagggc gtgcgacgat cggttacaaa atgcccttcc gagaccgtcc
      541 ggagagcact gatgccgtca tccgtcgtac caatggaggc tcaggtgcct gtctggggaa
      601 aatgcttata gaacatcatc gccttgaggc gccgctagga tgggcagcgt ttaggagggt
      661 gtgcacatgc tcgagaccat cgccggagcg ggggatgcgc tccttcagat cctcttgttg
      721 atattttctt acgccttcct tggagggacg ataaagtaca tcgatcaggc atatgacaag
      781 ggcatcggct cgttaaaggc cgccaagccc ctggccgtcg tctctgggat cctcatggga
      841 gtggtcatgg tcattgacgg ggggttctct acagctttct tccttgcgat gctgatcagc
      901 cttgtgctca cgaagaagat cgacaaccag agctttctgg tgggtaccat atccggggtg
      961 gccaccttcc tcgtgggcgg atcggtgtgg ggcatggaca tattgcccct gcccctggtg
     1021 gtcttcatca ttgccggtat agtggacgag ctggccgatg cgttagccca cgatcggaac
     1081 ctaggaaggg ctgcgggcct tcttctgcac taccgcccct tcagcgatat cgccctcgtg
     1141 gccttgatag tggccacccc ctttgactgg acatacctgg cgccctactt cgcgttcacc
     1201 ctcagctacc ttctgatggg aatgcgggag gaagggcagg tcggcgggtc ggtccgcggg
     1261 atcatccgca aggcgggcga cctccgcccc tggg
//
LOCUS       DAOSYN010000346         1287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_76801_length_1287_cov_3.195617, whole genome shotgun sequence.
ACCESSION   DAOSYN010000346 DAOSYN010000000
VERSION     DAOSYN010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76801_length_1287_cov_3.195617"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1279)
                     /locus_tag="PLJ11_08690"
     CDS             complement(113..1279)
                     /locus_tag="PLJ11_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HOO04783.1"
                     /translation="MRDDLIEDISCDGYNIPLFVFHGRWESIRTNIVFSNEDDLNSFV
                     ITLAQRSGKQISVASPILDGTTMEGHRIQATYSREVTTRGSTFTIRRFKNKPFTPTDL
                     LRYRTVSPEMMAYLWIGVENGESMILVGGTASGKTSTLNALAIFIPPGAKIVTIEDTR
                     EINLPHENWIPGATRTGVGEKDAEGRTAGEIDMYDLVRAALRQRPNYIIVGEVRGKET
                     YIMFQAMATGHSTYSTMHADSVKSMVNRLENPPINCPRILLTALRNVLIQSQVKVGNE
                     MVRRMKQIVEIVGYEPETNELITNTVYEWDPATDRFIFKGHAFLFDKIMEMRSLTNDE
                     MMAEFDRRVEVINYLVKKDVRNHREIWNIVNSYYKDGAKTLARLRADLAATEVG"
BASE COUNT          241 a          379 c          348 g          319 t
ORIGIN      
        1 ttgattacgc ggggaccagc ctctttggaa tgggcagggg taacgctctt cgttcgaaga
       61 taggacctct tggtcgagtc catgcgggta aagagatcgc tctcccccac catcatccca
      121 cctccgtggc agccaggtca gctctcagcc tggctagggt cttcgcaccg tctttgtagt
      181 aggagttcac tatattccag atttcgcgat ggttgcgcac atccttcttg accagatagt
      241 tgatgacctc tacacggcgg tcgaactccg ccatcatctc gtcgtttgtg agagacctca
      301 tctccatgat cttatcgaac aggaaggcat gtcccttgaa aatgaaccgg tcggtggccg
      361 ggtcccattc gtacacggtg ttcgtgatga gctcattggt ctccggctca tagccgacta
      421 tctccactat ttgcttcatg cggcggacca tctcgttccc gaccttgacc tgggattgga
      481 tcaggacgtt ccgcagggcg gttagcagga tccttgggca gttgatgggc gggttctcca
      541 gcctgttcac catgctcttg accgaatcgg cgtgcatggt ggaataggtg ctatgccccg
      601 tcgccatggc ctggaacatg atgtacgtct cctttccgcg cacctccccc acgatgatgt
      661 agttcgggcg ctgccggagg gcggcgcgca cgaggtcgta catgtctatc tccccggcgg
      721 tccgcccctc cgcgtccttc tcccccacgc ctgtccttgt cgccccgggg atccagttct
      781 cgtgtggaag gttgatctcc cgggtgtcct cgatggtgac tatcttggcc cccggcggga
      841 tgaatatggc gagtgcgttc aaagtactcg tcttcccgct ggccgtgccc ccgaccaata
      901 tcatggactc cccgttctcc acgcctatcc acagataggc catcatctca gggctgaccg
      961 tcctgtagcg cagaaggtcg gtcggggtga agggcttgtt cttgaaccgg cggatggtaa
     1021 aggtcgaccc tctcgttgta acttcccggg agtatgtcgc ctggatacgg tgcccctcca
     1081 tcgttgtacc atctaaaatg ggggaggcga ccgatatctg tttcccgctg cgctgagcaa
     1141 gagtgatcac gaatgaattt aggtcatcct catttgagaa aacgatgtta gttcgtatgc
     1201 tctcccacct cccatggaag acgaacaagg gaatattata tccgtcacac gagatgtcct
     1261 caataagatc atcccgcatc aggacat
//
LOCUS       DAOSYN010000347         1287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_76804_length_1287_cov_3.140422, whole genome shotgun sequence.
ACCESSION   DAOSYN010000347 DAOSYN010000000
VERSION     DAOSYN010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76804_length_1287_cov_3.140422"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /gene="arsB"
                     /locus_tag="PLJ11_08695"
     CDS             complement(<1..1039)
                     /gene="arsB"
                     /locus_tag="PLJ11_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOO04784.1"
                     /translation="MFIGVLLGTVVPGMADALSSLSIGTTSIPIAVGLILMMFPPLAK
                     VRYEDLPELLRMPGSRRMFSVSLSLNYIVGPLLMFALAWIFLPDHPGYRIGLILTGIA
                     RCIAMVLVWNQLAEGDTEYAAVLVALNSLFQIALYALYAYFLVFVLSDFLVPGSGVEV
                     DITVMEVAETVLIYLGIPFFGGMLTRRLLRPYKGAEWYDGRFMPVMGTVSLLALLFTI
                     VVMFSLKGEYIVQLPLDVLRIALPLLVYFIVMFLLSFTISIRLRFDYPHAAAQSFTAS
                     SNNFELAIAVAIGVFGIGSDVAFAAVVGPLIEVPALISLVNLALWFKKRYYDEDGRVR
                     ERNGKKRGFEGA"
BASE COUNT          309 a          366 c          393 g          219 t
ORIGIN      
        1 aagcgccctc aaaacctctt ttctttccat ttctttctct cacccgtcca tcctcgtcat
       61 agtatctctt cttgaaccaa agcgccaggt tcaccaggct tatgagggcg gggacctcga
      121 tcagcggccc gaccacggcc gcgaacgcta cgtccgagcc gatcccgaag acgccgatcg
      181 ccaccgcgat ggcgagctcg aagttgttgg acgacgcggt gaaggactgc gcggcggcat
      241 gcgggtagtc gaacctcaag cggatggaga tggtgaacga gaggaggaac attactatga
      301 agtacaccag aagggggagg gcgatgcgca gcacgtccag cgggagctgg acgatgtact
      361 cgcctttcag agagaacatc acaacgatgg tgaagaggag cgccagcagg gacaccgtcc
      421 ccatgaccgg catgaaccgt ccatcgtacc actccgcccc tttataggga cggaggaggc
      481 gacgggtcag catccctccg aagaacggga tccccaggta gatcagcacc gtctcggcca
      541 cctccataac ggtgatgtcg acctccactc cagaacctgg gaccaggaag tctgagagca
      601 cgaagacgag gaaataggcg tagagggcat agagcgcgat ctggaacagg gagttcaggg
      661 ccacgaggac ggccgcgtac tcagtgtccc cctcggcaag ctggttccac acgagcacca
      721 ttgcgatgca acgggcgatg ccggtgagga tgagcccgat gcggtacccc ggatggtccg
      781 gaaggaatat ccaagctagg gcgaacatca gcagcgggcc gacgatatag ttcagggaca
      841 gggagacgga gaacatccgg cggctcccgg gcatcctgag caattcggga aggtcctcgt
      901 accggacctt ggccagggga gggaacatca tcaggatcag cccgaccgcg atgggaatgg
      961 aggtcgttcc aatgctcaac gagctgaggg catcggccat accaggaacg acggtgccta
     1021 ggagcactcc gatgaacatt gccaaaaata tccacaaggt cagatagcga ttaaggaaag
     1081 gaaggcctgt cgtagttccg ctcagggcgg tcaccctcta tcaatagagt agttcaaaat
     1141 atattgaaat attttgttaa gcccctatgg caaccgtcgg gcaactttcg tcccgcgccc
     1201 atcggccgcc gctcgcctgc cgaccgggat gagcgatgga tgccttggaa ggaggggacc
     1261 gtcgcatcct gggttgcgtt acttccc
//
LOCUS       DAOSYN010000348         1269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_78331_length_1269_cov_4.467875, whole genome shotgun sequence.
ACCESSION   DAOSYN010000348 DAOSYN010000000
VERSION     DAOSYN010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78331_length_1269_cov_4.467875"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1269
                     /locus_tag="PLJ11_08700"
     CDS             <1..>1269
                     /locus_tag="PLJ11_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOO04785.1"
                     /translation="WVDHNEGPDPLLIVPTLTTALPDRLSWTLEPSCSRLDPNGSCSF
                     YLTFSAEADMYPAVIPMTVVFNITDMAANSSEEVAADIVLEARSAFGAIDRNNKILGV
                     WDNFLPAPLDGNWGAFGLSILIWVGIGLLALKGLEPALHRLARRTRTRWDKVVAEVIK
                     LPLLLLVLAYGIVSSLEILNLSPALLTDLELAYMIAVVLIASLLAYRVLVRIVAVYGA
                     ERSLNGRNGNKKALVNSVSLLGKVVIPVVAAFIIAGMLGMDLGNAILGVGFLGLVIGY
                     ATQSALGNIFAGLQLLLDRPFKVGDRVPLNDGHTARVLDVGLLTTKFLDLDTFEEVIM
                     PNSLIESQAIVNMNAPDARWKSSVKVRVSSDEDPKRIEELMMEAARRTPNILQGREAP
                     VVRVSEVGDGRMLLTIFIWIDDVANRHLART"
BASE COUNT          232 a          387 c          394 g          256 t
ORIGIN      
        1 atgggtggac cacaacgagg gcccagaccc gctgctgatc gtccccacgc tcaccaccgc
       61 ccttccagac cgactcagct ggacgctgga gccttcgtgt tcacggctgg accccaacgg
      121 cagctgcagc ttctatctca ccttcagcgc ggaggcggac atgtacccag ccgtcattcc
      181 gatgacggtc gtcttcaaca tcaccgacat ggcggctaac tcctcggagg aagtggctgc
      241 cgacatagtg ttggaggcga ggtccgcctt cggggccatc gatcgcaaca acaagatcct
      301 cggggtgtgg gacaacttcc tccctgcgcc tctggatggc aactgggggg cctttggcct
      361 tagcatcctg atctgggtgg gcattggcct actggccttg aaggggcttg agccagccct
      421 tcatcgattg gcccgaagga ccaggaccag gtgggacaag gtcgtcgcgg aggtgatcaa
      481 gctcccattg ctcctccttg tattggcata cggtattgtt tcctccctgg agatactgaa
      541 cctttccccc gcccttctga ccgacctcga gctggcatac atgatcgcgg tggtcctcat
      601 cgcctcgctg ctggcctacc gcgtgctcgt taggatcgtg gcggtctatg gcgcggagcg
      661 ctccctcaac ggccgcaatg ggaacaagaa ggccttggtg aactccgtgt ccctgctggg
      721 caaagtggtc atccccgttg tggccgcgtt catcatcgcc gggatgctgg ggatggacct
      781 ggggaacgcc atcctcgggg tcgggttctt agggctcgtc atcgggtacg cgacccagag
      841 cgccctcggt aacatcttcg ctggtttaca gctcctgctg gaccgcccgt tcaaggtcgg
      901 ggaccgggtg ccgttgaacg acggccatac cgcccgggtc ctcgatgtag ggctccttac
      961 caccaagttc ctggacctgg acacgttcga ggaggtcatc atgccgaact ccctgatcga
     1021 gagccaggcg atagtcaaca tgaacgcccc tgacgcccgc tggaagtcaa gcgtcaaggt
     1081 gagggtgtcc tcggacgaag atccgaagag gatagaggaa ttgatgatgg aggcagcccg
     1141 ccggacaccg aacatattgc aggggcgcga ggccccagtg gtcagggtct cggaggtggg
     1201 ggacggccgc atgcttctga cgatattcat ttggatcgac gatgtcgcga acaggcatct
     1261 ggcccgtac
//
LOCUS       DAOSYN010000349         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_78688_length_1265_cov_2.783471, whole genome shotgun sequence.
ACCESSION   DAOSYN010000349 DAOSYN010000000
VERSION     DAOSYN010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78688_length_1265_cov_2.783471"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLJ11_08705"
     CDS             complement(<1..292)
                     /locus_tag="PLJ11_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOO04786.1"
                     /translation="MIYMRLDAKDRIIISMYAEDPDVSQESIAKRVGLSQPSVAARIA
                     KLKEGGALASQFGIDPLRMGLYLAKVDISSTRPDEILGMFRGCPYFANGFTIS"
     gene            395..>1265
                     /gene="hcp"
                     /locus_tag="PLJ11_08710"
                     /gene_synonym="priS"
     CDS             395..>1265
                     /gene="hcp"
                     /locus_tag="PLJ11_08710"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOO04787.1"
                     /translation="MSMYCNQCQQTAKGIACTTRGVCGKTEDVQSLQEMLIYGLKGIA
                     AYAYHARVLGKRDEQIDAFMHEALFKTLTNVDFSTEDHWNMVLKAGIVNYRAMEMLQE
                     GNTEHFGDPAPTSVFTGVRKGPGILVTGHDLLDLEALLKQTEGTGVNVYTHGEMLPAH
                     AYPKLKKHSHLVGNYGSAWQKQKTEFAEFGGPILATTNCVLIPPESYNDRLYTTGITA
                     LDNVKHLERNDYSEIIEHAKRIGDLAERQGINVMTGFHHKAVLSLAPQIIDAVNTGKI
                     RRFFLIGGCDGATP"
BASE COUNT          292 a          387 c          353 g          233 t
ORIGIN      
        1 ccgagatcgt gaacccattg gcgaagtagg ggcatcctct gaacatgcca aggatctcat
       61 ctggcctcgt ggaggagata tcgaccttgg ccaggtagag ccccatcctc agggggtcga
      121 tcccaaactg gctcgccagc gcccctccct ccttgagctt ggctatccgg gcagccacgg
      181 acggctgcga aaggcccacc ctcttggcta tgctctcctg ggagacatca gggtcctcgg
      241 cgtacatgga gattatgatc ctgtccttag cgtccaaacg catgtagatc atccatttac
      301 ctattttaaa taaggaatct gtgcaatatc attattattc atagtaactg acttgatata
      361 ttataactta attgtacgga acaagggatg gaacatgtcg atgtactgca accagtgcca
      421 acagaccgcg aaggggatcg cctgcacgac ccgtggggtc tgcgggaaga ccgaggacgt
      481 ccagagcctg caggagatgc tcatctatgg gctgaagggc atcgccgcct acgcctatca
      541 tgcccgcgtc ctgggcaaga gggacgagca gatcgacgcc ttcatgcatg aggcgctgtt
      601 caagaccctg accaacgtgg acttctccac cgaggaccat tggaacatgg tcctcaaggc
      661 cggaattgtc aactatcggg ccatggagat gctgcaggag ggcaacaccg agcacttcgg
      721 cgatcccgcc cctaccagcg tcttcaccgg cgtgaggaag ggacctggta tattggtcac
      781 cggccacgac ctcctggacc tggaggcgct gctgaaacag accgagggca ccggcgtcaa
      841 cgtctacacg cacggcgaga tgctgccggc ccacgcctac cccaagctga agaaacactc
      901 ccacctcgtg ggcaactacg gctccgcctg gcagaagcag aagaccgagt tcgcggagtt
      961 cgggggaccc atccttgcca cgaccaactg cgttctgatc cccccggaga gctacaatga
     1021 ccgcctgtac accactggaa ttaccgccct ggacaacgtc aagcacctgg agcggaacga
     1081 ttactcggag ataatcgagc acgccaagcg catcggcgac ctggccgaga ggcagggcat
     1141 caacgtcatg accgggttcc accacaaggc ggtactttcg ttagccccgc agatcatcga
     1201 cgcggtcaat accggcaaga taaggcgctt cttcctgatc ggcggctgcg acggcgccac
     1261 gcccg
//
LOCUS       DAOSYN010000350         1250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_80011_length_1250_cov_2.994979, whole genome shotgun sequence.
ACCESSION   DAOSYN010000350 DAOSYN010000000
VERSION     DAOSYN010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80011_length_1250_cov_2.994979"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLJ11_08715"
     CDS             <1..852
                     /locus_tag="PLJ11_08715"
                     /inference="COORDINATES: protein motif:HMM:NF017482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOO04788.1"
                     /translation="TSVDPPTRSFTMRLGDAQLSLDYTVGIDENATRLYDQAKELRAK
                     GAGAKEAVEETRKAIERREARREGAALAKGRAAPTKRFWFESYKWFLTSGGRLVLAGR
                     DAKTNDQVVKRHLGERDRYAHADLHGAASVVLKDGAGASEEEMREVCQFALCHSKAWN
                     AGAAEGTAYWVLPDQVSKRPEAGEFAPRGAFIVRGKRNYAHHLPLEMAVAEVEVEGAR
                     KIMCAPRESVEGRTERYVLIVPGKMPRGKAAAALAKAFQVPEEEVSRILPPGDVEVRE
                     AHGVAME"
     gene            complement(859..932)
                     /locus_tag="PLJ11_08720"
     tRNA            complement(859..932)
                     /locus_tag="PLJ11_08720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(895..897),aa:Arg,seq:gcg)
     gene            complement(1086..1244)
                     /locus_tag="PLJ11_08725"
     CDS             complement(1086..1244)
                     /locus_tag="PLJ11_08725"
                     /inference="COORDINATES: protein motif:HMM:NF002242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HOO04789.1"
                     /translation="MATELVDKYPRAFSADFENNKVLVQRLTTVKSISMRNRIAGYIS
                     RYWQSKKF"
BASE COUNT          233 a          360 c          457 g          200 t
ORIGIN      
        1 acctccgtgg accccccgac gaggagcttc accatgcggc tgggcgacgc ccagctgtcc
       61 ttggactata cggtcggcat cgacgagaac gccacccgcc tctatgacca ggcgaaggag
      121 ctgcgggcca agggggccgg ggccaaggag gccgtggagg agacccgcaa ggccatcgag
      181 cggcgggagg ccagaaggga gggggccgcc ctggcgaagg ggcgggccgc cccgaccaag
      241 cgcttctggt tcgaatccta caagtggttc ctcacctccg gagggcggct ggtgctggcg
      301 ggcagggacg ccaagaccaa cgaccaagtg gtgaagaggc acctggggga gagggacagg
      361 tacgcccacg ccgacctcca cggcgccgcg tcggtggtgc tcaaggacgg cgccggggcc
      421 tccgaggagg agatgcggga ggtatgccag ttcgccctgt gccattccaa ggcctggaac
      481 gccggggcgg cggaggggac cgcgtactgg gtcctgccgg accaggtgtc caagaggccg
      541 gaggcggggg agttcgcccc ccgcggcgcc ttcatagtcc gggggaagag gaactacgcg
      601 caccatcttc ccttggagat ggcggtcgcc gaggtcgagg tggagggcgc tagaaagatc
      661 atgtgcgcgc caagggagag cgtggagggg cggacggagc gctatgtcct gatcgtcccc
      721 ggcaagatgc ccagagggaa ggccgcggcc gcccttgcca aggccttcca ggtcccggag
      781 gaggaggtgt cgaggatact gccccccggg gacgtggagg tcagggaagc gcacggagtg
      841 gcgatggagt gaggaaggcg cccgggccgg aattcgaatc cgggtctaga ggtccgcagc
      901 ctcttaggat atccaaacta ccccacccgg gcttggttct gtggacaata tgctccgatt
      961 actaaagcgt tgttatgtgc gttgttattt ccaccggtgg gacgccccca cctgtccaat
     1021 gcggaatagg gatttcatct gggatatgac gagcgaggcg aagcgcctcg ctgaaaggtg
     1081 cgatcctaga acttcttgga ttgccagtaa cggctgatgt acccggcgat gcggttcctc
     1141 atggagatgg acttcacggt ggtgagtctc tgcactagca ccttgttgtt ctcgaagtcg
     1201 gcactgaagg cgcgggggta cttgtcgacc agctccgtgg ccaccctctt
//
LOCUS       DAOSYN010000351         1249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_80078_length_1249_cov_4.665829, whole genome shotgun sequence.
ACCESSION   DAOSYN010000351 DAOSYN010000000
VERSION     DAOSYN010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80078_length_1249_cov_4.665829"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLJ11_08730"
     CDS             <1..798
                     /locus_tag="PLJ11_08730"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO04790.1"
                     /translation="DLMRMDGQASTVDGWLVSRMANRAGWVREAMKGYDIRTLANEVF
                     FEAFNDLRWYQRRGGGNAATVRRVMDILLPLMMPITPHLAEELWEMSGHEGFVSTTTY
                     PEALEGEIDPAKERAEDYLMALMEDANEILKVTGIRPRRMALFTSPAWKHKVLAMAVS
                     LHAKGELRVPTLTKMAMADEEVRVHGKEASDYARRLAEELMKRSSSEVQKMAHTFDEH
                     AFLEASKGFLEREYNCIVEVHGAGEHGADPQHKARQAAPGRPAIIVE"
     gene            complement(815..>1249)
                     /locus_tag="PLJ11_08735"
     CDS             complement(815..>1249)
                     /locus_tag="PLJ11_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOO04791.1"
                     /translation="ILEAETDHRIAQVEELADEVYHEDELFAPVASGSHRFERMVICP
                     CSESTLAKVSLGISDNLVTRVAAVCLKEGRRLIVVPRETPLSPAMLRNSLRLSEDGAV
                     ILPASPGFYPRPQSVEDMVDFVVGKVLDQLGQPHELFRRWGD"
BASE COUNT          238 a          392 c          437 g          182 t
ORIGIN      
        1 gacctcatgc ggatggacgg gcaggcgagc acggtggacg gctggcttgt gtccaggatg
       61 gccaaccgcg ccggctgggt ccgggaggcg atgaaagggt acgacattcg aaccctcgcg
      121 aacgaggtct tcttcgaggc cttcaacgac ctccgctggt accagcggag gggagggggc
      181 aacgccgcga ccgtccggag ggtcatggac atcctgcttc ccctcatgat gccgatcacc
      241 ccgcacctcg cggaggagct ctgggagatg tcagggcacg aggggttcgt ctccaccacg
      301 acatacccag aggcgctgga gggggagatc gacccggcca aggaaagggc cgaggactac
      361 ctcatggcac tgatggagga cgccaacgag atcctgaagg tgaccgggat caggccgagg
      421 aggatggcgc tcttcacctc ccccgcctgg aagcacaagg tcctggcgat ggccgtcagc
      481 ctccacgcca agggcgagct gagggtcccc acgctgacca agatggccat ggccgatgag
      541 gaggtgcgcg tgcatggcaa agaggcctcc gactacgccc ggaggctggc cgaggagctg
      601 atgaagcgct cctcctctga ggtccagaag atggcgcaca cgttcgacga gcatgcgttc
      661 ctcgaggcga gcaaggggtt cctcgaacgg gagtacaact gcatcgtcga ggtgcacggg
      721 gcgggggagc atggggccga tcctcagcac aaggccaggc aggcggcccc ggggcggccg
      781 gcgatcatcg tggagtaagc cggtccataa tgggtcagtc cccccaccgg cggaacagct
      841 catggggctg gcccagctgg tcgaggacct tcccgaccac gaagtcgacc atgtcctcaa
      901 cgctctgcgg cctggggtag aacccggggc tcgccgggag gatgacagcc ccgtcctcgg
      961 agaggcgcag cgagttgcgc agcatcgcgg ggctgagcgg ggtctcccgc ggcacgacga
     1021 tcaacctcct gccctccttc aggcagaccg cggccaccct ggtgaccagg ttgtccgaga
     1081 tcccgaggga gaccttggcc agggtggact cggagcacgg gcatatgacc atgcgctcga
     1141 agcggtggct gccggaggcc acgggggcga agagctcatc ctcatgatag acctcgtccg
     1201 caagctcctc cacctgggcg atcctgtgat cggtctccgc ctccaatat
//
LOCUS       DAOSYN010000352         1239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_81026_length_1239_cov_2.724662, whole genome shotgun sequence.
ACCESSION   DAOSYN010000352 DAOSYN010000000
VERSION     DAOSYN010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81026_length_1239_cov_2.724662"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..>1239
                     /locus_tag="PLJ11_08740"
     CDS             165..>1239
                     /locus_tag="PLJ11_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOO04792.1"
                     /translation="MRIAHVADTHVGFTAYRRTDPETGMNQREVDVYRAFERLVDSLI
                     AKRPDVVLHSGDLFDSVRPSNRALSVVLEQLLRLSREGIPIVLIAGNHSTPRLRETGS
                     VFRLFEHIDGVHPFYAPGMHRLELGDLTVTAFPHQDREGMMEALKGWRRGRARFEVGM
                     VHAGIQGLTRYSMGEANELNLPSSLLNLDVDYVAMGHFHEMERVTSNAWYSGSLERLS
                     FAEAGQEKGYLLVDLDRGKREFVRLETRPMYALGPIQAKGRGAGELQEEIAETLASVD
                     LEGAVVRLVVEDVPAALYRSLDLNALRAMAERAVHLELKFGIAQGPAAVQWRSASLGS
                     LEQEFQSYLSQVPVEGVDREALRV"
BASE COUNT          201 a          405 c          439 g          194 t
ORIGIN      
        1 aaggtgaagc gggacacgga gcagacgttc ttctgagccc ctcgcgggga aggagcggcg
       61 atgcccttgg acgggtgacc attccccatc caacaggggg agcgcccact ttcggccctc
      121 cccggcgaat ggttatttaa gcgccccccg ctacaccaca tcggatgagg atcgcgcacg
      181 tcgccgacac gcacgtcggc ttcaccgcct atcgccgcac ggacccggag acggggatga
      241 accagcggga ggtggacgtg taccgcgcct tcgagcgcct ggtcgactcg ctcatcgcca
      301 agcgcccgga cgtggtcttg cactccggag acctgttcga ctcggtccga ccgtccaacc
      361 gcgcgctctc cgtcgtgctg gagcagctcc tccgcctctc ccgggagggg ataccgatag
      421 tgctgatcgc cggcaaccac tccaccccca ggctgcggga gacggggagc gtgttccgcc
      481 tcttcgagca catcgacggc gtccatccct tctacgcccc gggcatgcac cggctcgagc
      541 ttggcgatct caccgtcacg gcgttccccc atcaggaccg ggaggggatg atggaggcct
      601 tgaaggggtg gaggcgcggc cgcgcccgct tcgaggtagg gatggtgcac gcgggcatcc
      661 aagggctgac cagatacagc atgggcgagg ccaatgagct caacctgccc tcctccctcc
      721 tcaatctgga cgtggactac gtcgccatgg ggcacttcca cgagatggag cgggtcacct
      781 cgaacgcctg gtacagcggc tccctggagc ggctctcctt cgccgaggcc ggccaggaaa
      841 aggggtacct gctggtggac ctcgacagag ggaagaggga gttcgtgcgc ctggagacca
      901 ggccgatgta tgcgctgggc ccgatccagg ccaaggggag gggggcgggg gagctgcagg
      961 aggagatcgc ggagaccctg gcctcggtgg acctggaagg ggccgtggtg cggctggtgg
     1021 tggaggacgt gcctgccgcc ctctaccgga gcctggacct caacgccctg cgggcgatgg
     1081 cggagagggc ggtgcatctg gagctcaagt tcggcatcgc ccaggggccc gccgcggtgc
     1141 agtggcgctc ggcctccctc ggctccctgg agcaggagtt ccagtcctat ctttcgcagg
     1201 tcccggtgga gggcgtggac agggaggcgc tgcgcgtcc
//
LOCUS       DAOSYN010000353         1232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_81666_length_1232_cov_2.916737, whole genome shotgun sequence.
ACCESSION   DAOSYN010000353 DAOSYN010000000
VERSION     DAOSYN010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81666_length_1232_cov_2.916737"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /gene="cas4"
                     /locus_tag="PLJ11_08745"
     CDS             complement(<1..507)
                     /gene="cas4"
                     /locus_tag="PLJ11_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOO04793.1"
                     /translation="MRETEPVIPARMLNEYVYCPRLCYLEYVQGEWRDSQDTVEGDNA
                     HRRVDREKGKVDEERFEATSVSMESEMEGIGCRVDLLRGEGGEVIPIDYKKGGAPDLP
                     SGAWEADMVQLCAQVLVLRDNGLACRGGHIYYAGSKKRVWIECDDELVERTREVVRTV
                     RNMVAEGAI"
     gene            complement(504..>1232)
                     /locus_tag="PLJ11_08750"
     CDS             complement(504..>1232)
                     /locus_tag="PLJ11_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04794.1"
                     /translation="PRDNLDSQGIREVEYRVEGKVVVPITDAVIVSERVRRKLMGIHK
                     RLMGDPSKVSPKFSGKDPDGVPLKGHPHCYICPLDTDRDGFVDHLSIRCHEPFTTMEL
                     KALDLLEGIPWKGGHTVLLTPVCTHKEVEERAGVMASRTPYVSPRHYRKGRGDFLEWM
                     KAELVRDIVDLGLPRPTSVEPVPFLGTARGDSPWFEFVRSRKEEAANFGYGFRVTFDV
                     PVPRMINAGYGAHYGLGLLMPEGP"
BASE COUNT          198 a          433 c          335 g          266 t
ORIGIN      
        1 gatggcccct tccgccacca tgttcctgac ggtgcgcacg acctcccggg tcctttcaac
       61 gagctcgtcg tcgcactcta tccagaccct cttcttagac ccggcgtagt agatgtgccc
      121 ccctctacat gccaatccgt tgtccctcag taccaggacc tgggcgcaga gctggaccat
      181 gtccgcctcc caggcacctg agggcagatc cggtgctcct cccttcttgt aatcgatggg
      241 gatgacctcg cccccctctc ccctgaggag gtccacccga cagcctatcc cctccatctc
      301 ggactccatg ctcaccgagg tggcctcgaa ccgttcttcg tcgaccttgc ccttttcgcg
      361 gtccaccctt ctatgggcat tgtccccctc gacggtgtcc tgggagtccc tccattcgcc
      421 ctgaacgtac tccaggtagc agagccgggg gcagtacacg tactcgttca acatcctggc
      481 cggtataacc ggttccgttt ctctcatggt ccttcaggca tgaggaggcc gagcccgtag
      541 tgcgcaccgt agcctgcgtt gatcattcgg gggacgggaa cgtcgaacgt gaccctgaac
      601 ccgtagccga agttcgccgc ttcctccttg cgggaccgaa cgaactcgaa ccagggggag
      661 tccccgcggg cggtccctag gaaggggacg ggctccaccg atgtcggcct tggcaacccc
      721 aggtccacga tgtcccggac cagttcggcc ttcatccatt ccaggaagtc cccccttccc
      781 ttcctatagt gtctggggct gacgtagggg gtcctgctgg ccatgacgcc cgcccgttcc
      841 tccacctcct tgtgggtgca taccggggtt agcaggaccg tatggccccc tttccaggga
      901 atgccttcga gcaggtccag ggctttgagc tccatggtgg tgaacggctc atggcaacgt
      961 atcgatagat ggtcgacgaa cccgtccctg tccgtgtcca gcgggcatat gtagcaatgg
     1021 ggatgcccct tcaacggaac gccgtccgga tccttgccgc tgaacttggg ggagaccttg
     1081 gaggggtccc ccatcaaccg cttgtggatc cccatgagct tccttcggac cctctccgag
     1141 acgatgacgg cgtccgtgat cggcacgacg acctttccct ccaccctgta ctccacctcc
     1201 ctgatgccct ggctgtccaa attgtccctc gg
//
LOCUS       DAOSYN010000354         1220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_82794_length_1220_cov_2.017167, whole genome shotgun sequence.
ACCESSION   DAOSYN010000354 DAOSYN010000000
VERSION     DAOSYN010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82794_length_1220_cov_2.017167"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLJ11_08755"
     CDS             <1..236
                     /locus_tag="PLJ11_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04795.1"
                     /translation="LFGAIYMGSEPLDYYFGPKQSSYSLGWGLISTFIGLVMLNVFVF
                     EGLSITILFAILLLVVGAVALVSFLKGGKKGGA"
     gene            238..792
                     /locus_tag="PLJ11_08760"
     CDS             238..792
                     /locus_tag="PLJ11_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04796.1"
                     /translation="MGAEEKYVQKGMDDRSLLEHIAMFVPGYHGYRERNIRRDVDQLI
                     RKNVSTVLKLGIKDLEWAYRETTNFGPSARVDEVNRIMMKTDKISQQIYHAKAGYSGI
                     WQGLKVKEGELSELLRFDASLIDQANKVKDSTGKIREHAKKHEFDQLFDLVDQYDIDL
                     DEIVTLISRRDEVILGLEKEGGEQ"
     gene            789..>1220
                     /locus_tag="PLJ11_08765"
     CDS             789..>1220
                     /locus_tag="PLJ11_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO04797.1"
                     /translation="MSETKDTMIIKWENPGPEDMIWRYPYAEIRFGAALVVREYEGAA
                     FLRDGKLFDVFEPGRHILSTQNLPILTKTYKLLMGYKESPFKADVIFFSKKMFNGNWG
                     LRTMVKAAQTMDAPIPLMANGIYQFRIEDPVLFLTQVVGGMS"
BASE COUNT          308 a          293 c          384 g          235 t
ORIGIN      
        1 tcctgttcgg cgcgatatac atgggttcgg aaccgctcga ttactatttc ggcccgaagc
       61 aatcgtccta ctcgctggga tgggggctca tatcgacgtt catcggactg gtaatgctca
      121 atgtcttcgt gttcgagggt ctgagcatca cgatcctgtt cgcaatactg ttactggtgg
      181 tcggagcggt cgcccttgtg agcttcctaa agggcggcaa gaaaggaggc gcgtgagatg
      241 ggtgctgaag aaaaatacgt tcaaaaaggc atggacgaca ggtccctgct cgagcatatc
      301 gcaatgttcg tccccggtta tcatggatac cgcgagagga acatcagaag ggacgtggac
      361 caactgatcc ggaagaacgt ctccacggta ttgaaattgg ggatcaagga ccttgaatgg
      421 gcgtacaggg agacgacgaa tttcggcccc tctgccaggg tggacgaggt gaaccgcatc
      481 atgatgaaga cggacaagat ctcgcagcag atctatcatg cgaaggccgg ttactcgggc
      541 atctggcagg gcctcaaggt aaaggaaggg gagctcagcg agctcctgag gttcgacgcc
      601 agtctcatcg accaggccaa caaggtgaag gattccacgg ggaagatccg agaacacgcc
      661 aagaagcatg agttcgacca gttgttcgac ctcgtggacc agtacgacat cgacctagac
      721 gagatcgtca ctttgatctc gcggagggac gaggtcatcc tcggccttga gaaggaaggt
      781 ggggagcaat gagcgaaacg aaagatacaa tgatcataaa atgggagaac cccggtccgg
      841 aggacatgat ctggagatac ccgtacgccg agattaggtt cggtgcggcc ctcgtcgtca
      901 gggaatatga aggcgcggcg ttcctgaggg acggaaagct cttcgacgtg ttcgagccag
      961 gaaggcacat actgtcgacg cagaacctgc cgatactgac gaagacgtac aaactgctga
     1021 tggggtacaa ggagtccccg ttcaaggcgg acgtcatatt cttctcgaag aagatgttca
     1081 acgggaactg ggggctcagg acgatggtca aggcggcgca gacgatggac gccccgatac
     1141 cgcttatggc caacggaata taccagttca ggatcgagga cccggtgctg ttcctgacgc
     1201 aagtggtcgg agggatgagc
//
LOCUS       DAOSYN010000355         1219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_82836_length_1219_cov_2.898625, whole genome shotgun sequence.
ACCESSION   DAOSYN010000355 DAOSYN010000000
VERSION     DAOSYN010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82836_length_1219_cov_2.898625"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1219)
                     /locus_tag="PLJ11_08770"
     CDS             complement(<1..>1219)
                     /locus_tag="PLJ11_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04798.1"
                     /translation="VLDRAGNAHIIYLDDIDDQLMYANNIDGSWNITVVDTFDVYATA
                     LTISDEGQLHAAYVGQNGKVNHAYRNNDVWLTEEIDNATGISPGVDIDIIGTRVCIAY
                     SSQSKELKFAIRDGANDWRTEEVDSSTNTVSWVSMDIDSYARVHITYYSNLNLNYALY
                     DGEWHLSILDTNSGLCSSIVSDLNDKQHVLYLKPIGESNALSYMTNSGARWVSEVVDE
                     GGAFDKVSSIAVDSEGNVHIAFFDEYKVNNVSCGQLCYANNVNGSWTVVTVDNSTSMV
                     GMNPSLAVDSKGWVHISYYDDTGLKVLKYINNVGGDWSIPVLLDQSGNSGMYSSLAID
                     ANDSVHVAFLNQGSKNLMYVTNARGFWDPDMIDESKTVSGRMSLALDPQGVPHIAYYR
                     DDGLAYAEQNGTA"
BASE COUNT          253 a          340 c          321 g          305 t
ORIGIN      
        1 catgccgtgc cgttctgttc ggcataggca aggccgtcat cgcggtagta ggcgatgtgc
       61 ggcactcctt gaggatcaag ggccagggac atccgtccgc tcactgtctt ggactcgtcg
      121 atcatatcgg ggtcccagaa gccgcgagcg ttggtcacgt acatgaggtt cttcgatccc
      181 tggtttaaaa aggcgacatg gaccgaatcg ttggcatcga tggccaacga gctgtacatc
      241 ccactgtttc cggactggtc caagaggacc ggtatgctcc aatcgccacc aacgttgttg
      301 atgtacttca ggaccttcag gccggtgtca tcgtagtagc tgatgtggac ccatcctttc
      361 gagtccaccg ccagggaggg gttcattccg accatggaag tggagttgtc gaccgttacc
      421 acggtccagc tgccattcac gttgttggca tagcacagct gcccgcagct gacgttattc
      481 actttatact catcaaagaa cgctatgtgc acgttgccct cggagtccac cgcgatcgag
      541 cttaccttat caaatgcccc gccctcgtcc acgacctcgc tcacccatct tgcccccgag
      601 ttggtcatgt aagagagcgc gttggattcg ccgatcggct tcagatagag cacgtgctgc
      661 ttatcgttca ggtcggacac tattgagctg cacagcccgc tattggtgtc caggatggat
      721 aggtgccatt ccccgtcgta caatgcgtag ttaaggttca gatttgagta ataggtgatg
      781 tgcacccggg catacgaatc gatgtccatg gagacccacg atacggtatt ggtgctgctg
      841 tcgacctctt ctgtcctcca gtcgttggca ccgtccctta ttgcgaactt caattccttt
      901 gactgactgg aatacgctat gcagaccctg gtgccaatga tgtctatgtc gaccccagga
      961 gagatgcctg tggcattatc gatttcctca gttaaccaga catcattatt gcggtaggcg
     1021 tgattgacct ttccgttctg accaacgtac gccgcatgga gctgcccctc atcggagatc
     1081 gtcaatgcgg tggcgtagac atcgaaggta tcaacaacgg tgatgttcca gctcccatcg
     1141 atattgttgg cgtacatcaa ttgatcgtcg atatcgtcca gatagatgat gtgcgcgttc
     1201 ccggcacggt ccaacacca
//
LOCUS       DAOSYN010000356         1219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_82838_length_1219_cov_2.828179, whole genome shotgun sequence.
ACCESSION   DAOSYN010000356 DAOSYN010000000
VERSION     DAOSYN010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82838_length_1219_cov_2.828179"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..>1219
                     /locus_tag="PLJ11_08775"
     CDS             260..>1219
                     /locus_tag="PLJ11_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HOO04799.1"
                     /translation="MTHPTEGAQDDSEGNYARHVAMLRAQMERGIVGGNRECPYFPCH
                     HPDQDCTFCFCPLYPCGDPLLGEYVVSQRTGGQVWSCQQCLWVHRGDVAASLYALMPE
                     LGPEGLDQGTRERLKGELEGKHRRRAKAIMVLGATSGAGKSLMVAALCRALRNRGLTV
                     APFKSQNMSLNSVVTEQGEEIARIQELQARAAGTRPRGELNPILLKPKGGVTSQIIVE
                     GRPYKDCTVQEYYSDFVRNEAPAIISRNLERLLRSNDVVVIEGAGSPAEINLRGRDVA
                     NMYVAKLAHADCLLVVNVERGGAFAYAVGTLALLDEEEKRLFKG"
BASE COUNT          231 a          369 c          422 g          197 t
ORIGIN      
        1 caccccgtcc tcctggcgga ggagggactc caggagcacc tttatggaga acgggagagg
       61 gtcggtcgaa gcccccagct tcttcaagga ataatagctc agggggccgt ctttaagctc
      121 gagaacgctc ttggtgtgga aggagtccga tgtcattgcc atcccgctcg gatggcagta
      181 agcgcgcgcg tccttaaaac attgcgggaa cgaaggggag ggaaaggcta ttatcgtatc
      241 agggggctct cccgattgga tgacccatcc aaccgagggg gcccaggacg attccgaggg
      301 gaactacgcg aggcacgtgg cgatgctgcg ggcgcagatg gaaaggggga tcgtaggcgg
      361 taacagggaa tgcccctatt ttccatgcca tcatcccgac caggactgca ccttctgctt
      421 ctgccccttg tacccctgcg gggacccgct cttgggggag tacgtggtat cccagcgcac
      481 gggcgggcag gtctggtcct gccagcagtg cctctgggtg caccgggggg acgtggccgc
      541 ctccctctac gccctcatgc cggagctagg gccggagggg ttggaccagg ggacgcggga
      601 aaggctcaag ggcgaactcg aggggaagca tcgccggagg gccaaggcga tcatggtcct
      661 gggggcgacc tctggggccg ggaagtccct catggtcgcc gcgctctgcc gcgccctgcg
      721 caaccgaggc ctgaccgtgg cccccttcaa atcccagaac atgtcgctca actcggtggt
      781 cacggaacag ggggaggaga tcgcccgcat ccaggagctc caggcccggg cggccggcac
      841 ccggcccagg ggggaactca accccatcct cctgaagccc aagggcgggg tcacctccca
      901 gatcatcgtg gagggaaggc cgtacaagga ttgtacggtg caggagtact attccgactt
      961 cgtccgcaac gaggccccag ccatcatatc ccgcaatctg gagcgcttgc tgcgatcgaa
     1021 cgacgtagtg gtcatcgagg gcgcgggctc ccccgcggag atcaacctac ggggaaggga
     1081 cgtggccaac atgtacgtgg ccaagctggc ccatgcggac tgtctcctgg tggtcaacgt
     1141 cgagcggggg ggcgccttcg cctacgcggt ggggaccctc gccctcctgg acgaggagga
     1201 gaagcgcctc ttcaagggg
//
LOCUS       DAOSYN010000357         1215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_83274_length_1215_cov_2.242241, whole genome shotgun sequence.
ACCESSION   DAOSYN010000357 DAOSYN010000000
VERSION     DAOSYN010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83274_length_1215_cov_2.242241"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1215
                     /locus_tag="PLJ11_08780"
     CDS             <1..>1215
                     /locus_tag="PLJ11_08780"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOO04800.1"
                     /translation="GPNKVYQKVKNGDSGLQLGFPVCPVCGAVRSPSLQLQSNDASID
                     EFFRKHREELHHENAGWYCLEAAFDADYLSLGPFASYEEAVSTSWAIVIGAMDHIELE
                     SDDLEVRAIPSEGTSFSAWIVDRHPGGSGLLDQIVQSWRAIIASALVLCTECPSQCES
                     SCQSCLRLYENQRDHPVLSRSLAMGPLAKLDARPEFGGGRGEAAQYVSPTGDPDTGSV
                     RTLAKKLEQAHLLGGAEMDPRIPVDSLGFSVRPDIHWLSGSGKHVCIYVDGSIHDVPE
                     VRSRDDHIRKTLRQKEGWAVHVIRNESVNDEAYVKDLIKRITNDLADGAYAPSESLRD
                     KVIKQIEGMGMPKPAEGQALNVTVLGASTRIRADFTWDLPGRVRMALLLLDAQGRTAE
                     GIDGEDVKYAMGK"
BASE COUNT          262 a          331 c          400 g          222 t
ORIGIN      
        1 ggtccgaaca aggtgtacca gaaggtgaag aatggcgata gcggccttca gctcggattc
       61 cctgtctgcc cggtctgtgg ggcagtgcgc tctccgagcc ttcagcttca atccaacgac
      121 gcgtccatcg atgagttctt ccggaagcac cgcgaggagc tgcaccatga gaacgcaggc
      181 tggtactgtt tggaggccgc tttcgacgcc gattacctgt cgctcggccc ctttgcatcc
      241 tacgaggagg cggtgagtac gtcctgggcc atcgtcatcg gggcgatgga ccatatcgaa
      301 ctggagagcg atgacctcga ggtgagggcg atccccagcg aagggacgag cttctcggcc
      361 tggatcgtgg accggcatcc cggtgggtcg gggctgctgg accagatagt acaaagttgg
      421 agggcgataa tagcctctgc actggtgctg tgcacggaat gcccttcgca atgcgagagc
      481 agttgccaat catgccttag gctctacgaa aaccaaaggg accatccggt cctttcgcgt
      541 agcctggcca tgggtccatt ggccaaactg gatgcgaggc ctgagttcgg tggcggtcgc
      601 ggagaggcgg cccaatatgt cagtccgacc ggggaccccg ataccggctc ggttagaacg
      661 ctcgcaaaga agctggaaca ggcacatctt ctcggagggg cggagatgga ccctaggatc
      721 cccgtcgact cactgggctt ctcggtgcgg ccggacatcc attggctatc cggttcgggg
      781 aagcacgtct gcatctatgt ggacggctcg atccatgatg tgccagaggt ccgaagcagg
      841 gacgatcata tccgaaagac cctccgccaa aaggagggtt gggcggtgca tgtgatcagg
      901 aacgagagcg tcaacgacga ggcgtatgtt aaggacctca tcaagaggat cactaacgat
      961 ctagcggacg gcgcctatgc accctctgaa tcgctccgag acaaggtcat caagcagata
     1021 gaaggcatgg ggatgcccaa acctgcagaa gggcaggcac tgaatgtgac ggtcctcggc
     1081 gcctccaccc ggataagggc ggacttcact tgggacctgc caggaagggt caggatggcc
     1141 cttctcctac tcgatgcgca ggggaggacg gccgagggga tcgacggtga ggacgtcaag
     1201 tacgcgatgg ggaag
//
LOCUS       DAOSYN010000358         1209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_83864_length_1209_cov_3.222704, whole genome shotgun sequence.
ACCESSION   DAOSYN010000358 DAOSYN010000000
VERSION     DAOSYN010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83864_length_1209_cov_3.222704"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PLJ11_08785"
     CDS             <1..1003
                     /locus_tag="PLJ11_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04801.1"
                     /translation="IELRGPVDERTSKRIESTIIHKETTPPKNVGIREADDIGRALRE
                     LDEQASSPKTRGTERTDMPMSSGSASDGLMGNIPTTVDVAVIPTEPGPPVKEEAASAP
                     EEALGTGPAIEDGAPRPTGEGIEVRQAAPADGGQVPSAEPVVSEAEEQRGPDTVGEPG
                     MPSPLGEDGAMKAGPETAVPMETVEVPISFEEEAAPKEDAKIGPQLATAPPALEEGAP
                     TVPSPPIAVQTLSFESDVPEEEGWKGVVPIAFLALGIVGYLSVLTLIALGVLGTAIGL
                     IGGAVSSLMIICGAIGIYPSARGKDGGEVFICPRCHENVGKRAGSCPACGAELASED"
     gene            1031..>1209
                     /locus_tag="PLJ11_08790"
     CDS             1031..>1209
                     /locus_tag="PLJ11_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO04802.1"
                     /translation="MHSCFEVSDEVTVVIDPHDGRSIGVKPPQLRADLVLISHDHFDH
                     NAIRVVKGDYTVIRDV"
BASE COUNT          247 a          348 c          418 g          196 t
ORIGIN      
        1 gatcgagctg cgtgggccgg tggacgaacg gacgtccaag cgcatcgagt ccaccatcat
       61 ccacaaggaa acgacccccc cgaagaacgt gggtatcagg gaagcggacg atattggtag
      121 ggcgctgcgg gaacttgacg agcaagcctc atccccaaag accagaggga ctgagaggac
      181 ggacatgccg atgtcctcag gatcagcctc ggacgggttg atggggaaca tccctaccac
      241 agtcgatgtg gcggtcatcc ccaccgagcc cgggcctccg gtgaaagagg aggcggcctc
      301 agcgccggag gaggcgttgg gaactggacc tgcgatcgag gatggagcgc cgcgaccgac
      361 cggcgagggg attgaggtac ggcaggcggc cccggcagat gggggtcagg tcccctcggc
      421 cgaacccgtc gtctcggagg ctgaggagca gagaggaccg gatacggtcg gtgaacctgg
      481 aatgccgtct cccctcgggg aggacggggc aatgaaggcc gggccggaaa cggccgttcc
      541 catggagacc gtcgaggtcc ctatttcctt cgaagaggag gccgccccca aggaagatgc
      601 caagatcggc cctcagctag cgacggcccc tccagcgctg gaggaaggcg caccaacggt
      661 tccctcccct ccgatcgccg tccaaacgct gtcctttgaa agcgatgtcc cggaagagga
      721 aggatggaaa ggtgtcgtcc ccatcgcctt cttggcgttg ggcatcgttg gctacctctc
      781 cgtcctgacg ctcatagcgc tgggggtcct cggcacagca atcggcctaa tagggggggc
      841 ggtgagttcg ctgatgataa tctgcggagc gattggcatc tatccttccg caagagggaa
      901 ggacggcggc gaggtcttca tttgcccgcg atgccatgag aacgtaggga agcgggccgg
      961 cagctgcccc gcgtgcgggg ccgagctcgc ctccgaggac taggtggggc agatgcggct
     1021 catatggcac atgcattcct gcttcgaggt gagcgatgaa gtgaccgtgg tcatcgaccc
     1081 tcatgacggc cgttcgatag gggtgaagcc gcctcagctc agggcggacc tggtgctcat
     1141 cagccatgac catttcgatc ataacgcaat cagggtggtg aagggcgatt acaccgtgat
     1201 cagggacgt
//
LOCUS       DAOSYN010000359         1208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_83954_length_1208_cov_2.984389, whole genome shotgun sequence.
ACCESSION   DAOSYN010000359 DAOSYN010000000
VERSION     DAOSYN010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83954_length_1208_cov_2.984389"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLJ11_08795"
     CDS             <1..822
                     /locus_tag="PLJ11_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOO04803.1"
                     /translation="DDGAWDVAALAALDIPILQGLCLTWGRAEWEASDDGMSPLDVAT
                     QVAVPEFDGRIITTAFSFKETGEDVKVMCSRKGKRINMTIACAMVDRYIPDADHYLSV
                     VEEMKERVLDNAAKYTDYDVNVDINHADNPTTGNFYLTVTGLSAENGDDGSVGRGNRV
                     NGLITPYRPMSMEASAGKNPITHVGKMYNILANWIANEIVKEGKGNVIEADVRILSQI
                     GRPISDPETCSVQLFMAPGSDTAKWEKEALAIADGWLEDIGKVTEKVVKGQVTVF"
     gene            826..>1208
                     /locus_tag="PLJ11_08800"
     CDS             826..>1208
                     /locus_tag="PLJ11_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04804.1"
                     /translation="MKLVDLPRYGPVHRFGNHHVYRTLMVLSDQKRKGRKQIADAVGI
                     GEGSMRTIIEYLRELDMIDVRQTGVKITRHGNAFLSELPIKMANIGQGSIAVGKECVA
                     VQMSNNGDKIRMGVEQRDSAIMAGA"
BASE COUNT          275 a          351 c          396 g          186 t
ORIGIN      
        1 gacgacggtg cctgggacgt cgccgcgctc gcggccctcg acatcccgat cctccaggga
       61 ctgtgcctga catggggacg cgccgagtgg gaggcgagcg acgacggcat gtcccccctg
      121 gatgtcgcaa cgcaggtcgc ggtgccggag ttcgacggtc gcatcatcac gacggccttc
      181 tccttcaagg agaccggcga ggacgtaaag gtcatgtgct cccgcaaggg gaagaggatc
      241 aacatgacca tcgcatgcgc gatggtcgac cgttacatcc cggacgcaga ccattacctc
      301 agcgttgtcg aggagatgaa ggagagggtg ctggacaatg ctgcgaagta cacggactat
      361 gatgtgaacg tggacatcaa ccatgccgac aaccccacaa ccggcaactt ctatctgacg
      421 gtgacggggc tcagcgccga gaacggggac gatggctccg tcggccgcgg gaacagggtg
      481 aacgggctga tcacccccta ccgccccatg tccatggagg cctccgccgg caagaacccc
      541 atcacccatg tgggcaagat gtacaacatc ctggccaact ggatcgcgaa cgaaatcgtg
      601 aaggagggca agggcaacgt gatcgaggcc gatgtgcgca tcctatccca gatcggccgt
      661 ccgatcagcg accccgagac atgcagcgtc cagctgttca tggcgcccgg ttccgacacc
      721 gcgaaatggg agaaggaggc cctggcgatc gcagatggat ggctcgagga cattggcaag
      781 gtaacggaga aagttgtaaa agggcaggtc acggtattct aagacatgaa gctcgtcgac
      841 ctgccccggt acggaccggt gcaccgcttc gggaaccatc acgtttaccg cacgctcatg
      901 gtcctctcgg accagaagcg caaggggcgc aagcagatag cggacgcggt gggcattggg
      961 gaggggagca tgcggacgat aatcgaatac ctccgcgaac tggacatgat cgatgtccgc
     1021 cagacgggcg ttaagatcac ccgtcacggt aacgccttcc tgtccgaact gccgatcaag
     1081 atggccaata tcgggcaggg ctccatcgcc gtgggcaagg agtgcgtggc cgtgcagatg
     1141 agcaacaatg gcgacaagat caggatgggg gtcgagcagc gggactcggc catcatggcc
     1201 ggggccga
//
LOCUS       DAOSYN010000360         1206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_84170_length_1206_cov_3.339705, whole genome shotgun sequence.
ACCESSION   DAOSYN010000360 DAOSYN010000000
VERSION     DAOSYN010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84170_length_1206_cov_3.339705"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLJ11_08805"
     CDS             <1..860
                     /locus_tag="PLJ11_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine(15) transglycosylase"
                     /protein_id="HOO04805.1"
                     /translation="YTGPETLNRPVFLRYQKRLAERYAHPATEVGVTFEEAEKPYSRT
                     LRPLIDSILAVSDAHFHVISPFGPVPIELDETYPISQSLFPAQRDSQTVERTKELMEV
                     LAHTHRYKMALIWDGDGTLEFLRTFAPGKGTFDIDMARVRAVADLQFGRGAAAALLDG
                     EVSLVKSKNVDRVRNVLVDGEHVLSMRAEDGFFTVRPAGARRLLRGFPSPRLRVMVQD
                     DAVEFNRQGKNVFCPFVIDADPDIHPQEEVLVVDKDDALVAIGRAQMTRGEMLSFRKG
                     VAVKVREGV"
     gene            complement(857..1084)
                     /locus_tag="PLJ11_08810"
     CDS             complement(857..1084)
                     /locus_tag="PLJ11_08810"
                     /inference="COORDINATES: protein motif:HMM:NF011470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HOO04806.1"
                     /translation="MQASCLLELDMGSEERARTINRSLEMDNGDHARSWVEGPVLKVD
                     CKAEGLMSLLRTIEDLMACLKVAEEITDVED"
BASE COUNT          217 a          355 c          406 g          228 t
ORIGIN      
        1 tctacaccgg ccccgagacg ctgaatcggc ccgtgttcct gagataccag aagcggttgg
       61 cggagcgcta tgcgcatccg gcgacggagg taggcgtcac cttcgaggaa gcggagaagc
      121 cctattcccg gacgctgcgc cccctcatcg attccatcct ggcggtcagc gatgctcatt
      181 ttcacgtcat ctccccattc ggtccggtgc caatcgagct ggacgagacc tatccgatct
      241 cccagtccct gttcccggcc caacgggact cccagacggt ggagcggacc aaggaactga
      301 tggaggtatt ggcgcatacc cacaggtata aaatggccct gatctgggat ggtgatggga
      361 ccctggagtt cctgaggacg ttcgccccgg ggaaagggac attcgacatc gacatggcca
      421 gggtcagggc ggtggcggac cttcagttcg ggaggggggc tgcggccgcc ctgctcgatg
      481 gggaggtgtc cctagtgaag tcgaagaacg tggaccgcgt gcggaatgtg ctggtcgacg
      541 gcgagcacgt cctgtccatg cgggccgagg atggcttctt taccgttagg ccggcagggg
      601 cccgaaggct gctgagaggg tttccctccc cccggctgcg ggtaatggtc caggacgatg
      661 ccgtggagtt caataggcag gggaagaacg tattctgccc cttcgtgatt gatgccgacc
      721 cggacatcca tccgcaggag gaggtgctgg tcgtggacaa ggacgacgcc cttgtggcca
      781 tcggccgggc ccagatgacc aggggggaga tgctcagctt caggaagggc gtggcggtca
      841 aggtgcgaga gggcgtctag tcctccacat cggttatctc ctccgcgacc ttcaggcagg
      901 ccatgaggtc ctcgatcgtg cgcagcaggg acatcaggcc ttcggccttg cagtcgacct
      961 tgagcaccgg cccctccacc caggacctgg cgtggtcccc gttgtccatc tccagggagc
     1021 ggttgatggt tcgggcccgt tcctcgcttc ccatgtccag ctcgagaagg cagctcgctt
     1081 gcacgaaggg tcgatgcccg gggagggata aaatcccttt tgtcgtggga gcgaggagag
     1141 ggatgatagc cccgtccaga ttcgaactgg agtcccctgc tccaggggca gggatgattg
     1201 cccact
//
LOCUS       DAOSYN010000361         1204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_84342_length_1204_cov_3.856397, whole genome shotgun sequence.
ACCESSION   DAOSYN010000361 DAOSYN010000000
VERSION     DAOSYN010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84342_length_1204_cov_3.856397"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /gene="amrB"
                     /locus_tag="PLJ11_08815"
     CDS             <1..443
                     /gene="amrB"
                     /locus_tag="PLJ11_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOO04807.1"
                     /translation="TAHLYEHSLEVQLPFLQYLFPDIKIVPIVMGRQDIVMARETAKV
                     LREACAGKDVIFLASSDLSHYVPPEVARERDALALDRVLHLDAEGLIDVVEANDISMC
                     GYGPVATMLMACGGTKARLLHYGSSGDVRPMRDVVGYASLVVER"
     gene            525..>1204
                     /locus_tag="PLJ11_08820"
     CDS             525..>1204
                     /locus_tag="PLJ11_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04808.1"
                     /translation="MDGTKTCPYCGRLASADAQFCQGCGRSISSPPQYAEQPAQQYNQ
                     PPQQGGWQQPPPSYGAPGQPYGQSFGGAKGQIAGAIQNRNVTDEVMSGIWILWPIFAT
                     VVGVILMVVLAIFIGLVGLVIGAVITLVLYCVLNYKLLKRMDEHRKREMALRMAIISY
                     LKDKGAETQRAQLIVNEVSSLESINQEAMYKEKERTPVLWTIISLFPLLNLIVMYFLA
                     EFPFDHDKR"
BASE COUNT          239 a          367 c          380 g          218 t
ORIGIN      
        1 gcaccgccca tctctacgag cacagcctgg aggtgcagct gccgttcctc cagtacctct
       61 tcccggacat caagatcgtg cccatcgtca tggggcgcca ggacattgtc atggccaggg
      121 agaccgccaa ggtgttgcgg gaggcctgcg ccggaaagga cgtgatcttc ctggcctcct
      181 ccgacctctc ccattacgtt cccccggagg tcgcccggga gagggatgcg ctggcattgg
      241 acagggtcct gcacctggac gccgaagggc tgatcgacgt cgtggaggcc aacgacatca
      301 gcatgtgcgg gtacgggccg gtcgccacca tgctcatggc ctgcgggggc accaaggccc
      361 gtcttctgca ctatggaagc tcgggggacg tccggccgat gcgggacgtg gtgggctacg
      421 cgtccctcgt cgtggagcga tgaccgttcc atcgatgcta taattatatg agaaagagca
      481 tatacggatt ggcctaggtc aggccatgga acggagagat tcaaatggac ggaacgaaga
      541 catgtcctta ctgcgggagg ttggcaagcg ccgacgccca gttctgccag ggctgcggaa
      601 ggagcatcag ctccccgccc cagtatgctg agcagccggc gcagcagtac aaccaaccgc
      661 ctcagcaggg aggttggcag cagccgcccc cctcctacgg agcccccggg cagccctacg
      721 ggcagtcctt cggcggggcc aaggggcaga tcgccggggc gatacagaat cgcaatgtga
      781 cggacgaggt catgagcggg atctggatcc tgtggccgat attcgccacc gtggtcgggg
      841 taatactcat ggtggtcctc gcgatcttca tcgggctcgt tggcctcgtc attggcgcag
      901 tgatcaccct cgtgctctac tgcgttttga actacaagct cctgaagagg atggatgagc
      961 atcggaagcg cgagatggcg ctccgcatgg cgatcatctc ctatctcaag gacaagggcg
     1021 ccgagaccca gcgggcccag ctcatcgtca acgaggtgtc ctccttggaa agcatcaacc
     1081 aggaggcgat gtacaaggag aaggagagga cccccgtcct ctggaccata atcagcctct
     1141 tcccgctgct caacctgatc gtgatgtact tcctggccga gttcccgttc gaccacgaca
     1201 agcg
//
LOCUS       DAOSYN010000362         1189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_85836_length_1189_cov_8.237213, whole genome shotgun sequence.
ACCESSION   DAOSYN010000362 DAOSYN010000000
VERSION     DAOSYN010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85836_length_1189_cov_8.237213"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1003)
                     /locus_tag="PLJ11_08825"
     CDS             complement(86..1003)
                     /locus_tag="PLJ11_08825"
                     /inference="COORDINATES: protein motif:HMM:NF033579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOO04809.1"
                     /translation="MSRRRGRFTENKILRAVRELKARLVRLAFPLYRTVRSNHLYTQH
                     QHLVLLVLRQWMGKSYREFCEWMGVCTKVLRLLGIVRMPHFTTLQKFSGRLDGDLLDR
                     LLGSYAEQLADIQLAVDSTGFSCTSASHYFVEVLKRSREKGAEIGMRAIRRHLKQTIG
                     VDVDTQLILAMHDRVGPANDFPDMIPLLQKVPGTARVRAVLADKGYDSEEIRRFIWYS
                     MKAESHIPLRSSRGARVNHIQRRKQAAEFDERKYHRRALVETVHSVLKRVMRDDVLAR
                     SRSGQHVELELRTIAYNARRAAIMGYGFY"
BASE COUNT          236 a          384 c          309 g          260 t
ORIGIN      
        1 aaaacatcgg tataatacag ggtcagtatc ggttttaata actagcctga cgatttactg
       61 atgttattcg atgctggcta tgtgctcagt agaaaccata acccatgatc gccgccctcc
      121 tcgcgttgta cgctatcgtc ctcagttcca gttcgacgtg ctgcccggac ctgctccggg
      181 ccagcacgtc gtcgcgcatc acgcgcttca ggaccgaatg caccgtctcc accagcgccc
      241 gccggtgata cttgcgttca tcgaactccg ccgcctgctt tcggcgctgg atgtggttca
      301 cccgggcgcc cctggacgat ctgagcggaa tatgcgactc cgccttcata gagtaccaga
      361 tgaaccggcg gatctcctct gagtcgtatc ctttgtcggc cagcaccgcc cgcacccggg
      421 cggtgcctgg caccttctgc agcagcggga tcatgtccgg gaagtcgttg gcagggccga
      481 cgcggtcatg catcgccaat atcaactggg tatccacatc cacgcctatc gtctgcttca
      541 gatgccggcg gatcgccctc atcccgatct ccgccccctt ctccctgctc cgcttcagca
      601 cctccacgaa ataatgactg gccgaggtgc agctgaagcc ggtggaatcc acggccaatt
      661 gaatatccgc aagctgctcg gcatacgagc cgagcagcct gtccagcagg tcgccgtcca
      721 gcctcccgga gaacttctgc aaggtcgtga aatgaggcat gcgaacgatg cccaagagcc
      781 tcagcacctt cgtgcacacg cccatccatt cgcagaactc ccggtaggac ttgcccatcc
      841 attgccggag aacgagcagc accaggtgct ggtgctgcgt gtacaggtgg ttcgatctca
      901 cagtccggta cagggggaag gccaggcgta ccagcctggc cttcagctcc ctcaccgccc
      961 gaagtatctt attctcggtg aatcttcctc ttctcctcga cacccaggca tcccatcccc
     1021 gggggcaggt ttgtcttgct acgatcgggc ctgcccccaa gggactggac agtgtcgagg
     1081 ctaattaatt ttatcattat tcgttcaata aaatgcgatg gtttctacag agcaaagtag
     1141 gggaaccctt tttatcccgc ggcaggatga cctacctatg ctgcccgag
//
LOCUS       DAOSYN010000363         1176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_87161_length_1176_cov_3.578055, whole genome shotgun sequence.
ACCESSION   DAOSYN010000363 DAOSYN010000000
VERSION     DAOSYN010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87161_length_1176_cov_3.578055"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLJ11_08830"
     CDS             complement(<1..293)
                     /locus_tag="PLJ11_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOO04810.1"
                     /translation="MPVTGKVMFQGVTSGAGKTTLTAALCRHLALQGREVVPFKAQNV
                     SLNSFVTADGREIAIAQAYQAWACGLEPEADMNPVLVKPKGNGRCQIMLGGRP"
     gene            complement(277..>1176)
                     /locus_tag="PLJ11_08835"
     CDS             complement(277..>1176)
                     /locus_tag="PLJ11_08835"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobQ"
                     /protein_id="HOO04811.1"
                     /translation="KGVIINNMCPGGGSLGPALEIIHTLTGVPVIGEVPHVPDLYLPM
                     EDSMDLDHREEGEGGTVIAVVRLPMISNFTDLDALALEEGVRVAYVRSPAELEGADAI
                     VLPGTKNTVQDLLWLHRSGMAAAIKERAGKVPILGICGGYQMLGRTISDVRGLEGGAP
                     SEVEGLGLLDAATVFDAYAKRTVQVTGSLAEGGGEVRGYEIHMGRTEGRSPPLFLLAP
                     DGRGEGAWDRERKVMGTYLHGAFDLPAFRRLFLSQAGRKDAVPGPSGDHDAQVEASLD
                     RLAFEVEGHLDLDALLGGDARDG"
BASE COUNT          166 a          450 c          376 g          184 t
ORIGIN      
        1 cagggcctgc ccccaagcat gatctggcat cgcccgttcc ccttcggctt gaccagcacg
       61 gggttcatgt cggcctcagg ctccagcccg caggcccacg cctggtacgc ctgggcgatg
      121 gcgatctccc tcccgtccgc ggtgacgaag gagttgaggg agacgttctg ggccttgaac
      181 gggacgacct cccggccttg cagggctaga tggcggcaca gcgccgcggt cagggtcgtc
      241 ttccccgcgc cggaggtgac cccctggaac atgaccttac ccgtcacggg catcaccccc
      301 gagcagggca tccaggtcca gatgcccctc cacctcgaac gccaggcggt ccagcgaggc
      361 ctcgacctgc gcgtcgtggt ccccggacgg cccggggacg gcatccttcc ggcccgcctg
      421 cgagaggaag agcctgcgga aggccgggag gtcgaaggcc ccgtggaggt acgtccccat
      481 gaccttccgt tccctgtccc aggccccctc cccgcgcccg tccggcgcca gcaggaagag
      541 cgggggggag cggccctccg tccgccccat gtggatctcg taccccctca cctcgccccc
      601 gccctccgcg agggagccgg tcacctgcac cgtccgcttg gcgtaggcgt cgaagaccgt
      661 ggcggcgtcc aacagcccga ggccctcgac ctcgctgggg gccccgccct ccagcccccg
      721 gacgtccgag atggtcctgc cgagcatctg gtacccgccg catatgccga gtattggcac
      781 cttccccgcc ctttccttga tggccgcggc catccccgac ctgtggagcc agagcaggtc
      841 ctgcaccgtg ttcttggtcc cgggaaggac gatggcgtcc gccccttcca gctcggccgg
      901 ggagcggacg tatgccaccc tgaccccctc ctccagggcc agcgcgtcga ggtcggtgaa
      961 gttggagatc atcggcagcc ggacgacggc tatgaccgtg ccgccctccc cctcctcccg
     1021 atggtccagg tccatcgagt cctccatcgg gaggtagagg tccgggacat gggggacctc
     1081 cccgatcacc ggcacccctg tcagcgtgtg gatgatctcg agggccggcc cgagggaccc
     1141 gccgccgggg cacatgttgt tgatgatcac cccctt
//
LOCUS       DAOSYN010000364         1176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_87191_length_1176_cov_2.473684, whole genome shotgun sequence.
ACCESSION   DAOSYN010000364 DAOSYN010000000
VERSION     DAOSYN010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87191_length_1176_cov_2.473684"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1176
                     /locus_tag="PLJ11_08840"
     CDS             <1..>1176
                     /locus_tag="PLJ11_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOO04812.1"
                     /translation="IQVAPPDEDGYCNLGISVDVTLSAAMNADTVIAEVNPNMPRVGG
                     DSRLHIREIDHVVESDQPLLEYRGEGEVARETPRIGKYVADLVPDGATIQIGYGSIPD
                     AILPFLEEKRDLGVHTEMFSDGLIDLVEKKVITGRRKGFHEGKVVSSFCMGTKRLYDY
                     IDGNEMLEMYPSAYTNDPCIIARNRNMIAINSALEVDLTGQVCSDQIGDRFYSGIGGQ
                     ADFTRGAARSPGGKAIIALPATAQHYTVSRIVPTLSTGGGVTTSRGDVQFVVTEFGVA
                     DLRGKSIMERALALIGIAHPRFRAELLMHAKSLHYVYQDQMEAGYVGVYPEQYETVKV
                     IADREMYFRPAKPTDEPLLKEMFYSLSKDTVYNRFMGLKRSMPHRELQRIVNIDYDTR
                     "
BASE COUNT          236 a          373 c          384 g          183 t
ORIGIN      
        1 catccaggtc gccccgccgg acgaggacgg gtattgcaat ctcgggatct ccgtcgacgt
       61 cacgctgtcc gcggccatga acgcggacac cgtgatcgct gaggtgaacc cgaacatgcc
      121 acgggtgggc ggggatagcc gattgcacat cagggagatc gaccacgtgg tggagagcga
      181 tcagcccctg ctggaataca ggggcgaggg ggaggtggcc cgggagaccc cccgcatcgg
      241 caagtacgtg gcggacctcg tcccggacgg ggcgaccatc cagatcgggt acggtagcat
      301 cccggacgcc attctgccgt tcctcgagga gaagagggac ctcggggtcc acaccgagat
      361 gttctcggac gggctcatcg acctggtgga gaagaaggtc ataaccggca ggaggaaggg
      421 cttccacgag gggaaggtcg tctcctcctt ctgcatgggg accaagcgcc tctacgacta
      481 catcgacggg aacgagatgt tagagatgta cccctcggcc tacacgaacg acccgtgcat
      541 catcgccagg aacaggaaca tgatcgccat caactccgcc ctcgaggtgg acctcacagg
      601 ccaggtctgc tccgaccaga tcggcgaccg cttctattcg ggcatagggg ggcaggccga
      661 cttcacccgg ggggcggccc gctcccccgg cgggaaggcc atcatcgccc tgccggccac
      721 cgctcagcat tacaccgtgt cccgcatagt ccccaccctg tccaccgggg gcggggtgac
      781 caccagcagg ggggacgtgc agttcgtcgt caccgagttc ggggtggctg acctccgggg
      841 gaagagcatc atggagcggg ccctcgccct catcggcatc gcccaccccc gcttccgcgc
      901 cgagctgttg atgcacgcca agtcgctcca ctacgtctac caggatcaga tggaggccgg
      961 gtacgtcggc gtgtacccgg agcagtatga gacggtgaag gtgatcgcgg acagggagat
     1021 gtacttccgc ccggcgaagc ccacggacga gccgttgctc aaggaaatgt tctattccct
     1081 gtccaaggac acagtgtaca acaggttcat ggggctgaag cgcagcatgc cccaccggga
     1141 gctgcagcgc atcgtgaaca tcgactacga tacacg
//
LOCUS       DAOSYN010000365         1174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_87423_length_1174_cov_1.523682, whole genome shotgun sequence.
ACCESSION   DAOSYN010000365 DAOSYN010000000
VERSION     DAOSYN010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87423_length_1174_cov_1.523682"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..>1174)
                     /locus_tag="PLJ11_08845"
     CDS             complement(135..>1174)
                     /locus_tag="PLJ11_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04813.1"
                     /translation="GGDSHVVPDQRGNFKIGPERVVPLSVASAAHPWLKERMGATGET
                     NYYLPVERGTPKLSPKEREVYEFVKANPLCTLEEIRRGAPEVYTYRDVVKHLKDRGFL
                     QMTGLTATDFMHVQGHFTRFDVEAARLGVQFIADRARMTVGECLESFQQEFVTRVGEE
                     VMKKIVLDESGEMPDSPGFNYLIRAAVNGNPGSGLVLRTRLDRPLVGIGAPTPIYMPP
                     LEKRMDVRVVIPRDHDVGNAVGAVCSKISETVTAQVYPNMDNVFYVLGPFGSTGTYSH
                     VEQAISSARRQAEEYVWNRAYEAGAENIKVRSDVYENKFYGGDNVEGEQTAWVDIVAR
                     ATGDPAAKRGK"
BASE COUNT          184 a          405 c          353 g          232 t
ORIGIN      
        1 ctaaccccta tgtggcaatc gttgggcagc tttcgtttcg cgcccatcgg ccggcgcccc
       61 ggtcgccgtt cgcctgccgt ccgggatgag cgatggatgt cttgagagga agggacgctg
      121 cacccttggc tgcgtcactt cccccttttg gccgcgggat cgccggtcgc ccgggccacg
      181 atgtccaccc aggcggtctg ctcaccctcg acgttatcgc ccccgtagaa cttgttctcg
      241 tagacgtcgc tgcggacctt gatgttctcg gcccctgcct cgtacgcgcg gttccacaca
      301 tactcctcgg cctgccgccg ggcggatgat atggcctgct ccacatgact gtacgtaccg
      361 gtcgagccga agggaccgag gacgtagaag acgttgtcca tattgggata gacctgggcg
      421 gtgacggtct cggagatctt actgcatacc gccccgacgg cgttgccgac gtcgtggtcc
      481 cttggaatga ccaccctgac gtccatgcgc ttctcgagcg ggggcatgta gatcggggtc
      541 ggcgccccta tcccgaccag cggccggtcc agccgggtcc tgaggaccaa gccgctcccc
      601 ggattcccat tgaccgcggc gcggatgaga tagttgaagc ccggactgtc cggcatctct
      661 cccgattcgt caaggacgat cttcttcatt acctcctccc cgacccgggt cacgaactcc
      721 tgctggaagg actccaggca ttccccgacg gtcatacggg cccgatcggc gatgaactgc
      781 acgcccagcc gggccgcctc cacatcgaag cgggtgaagt ggccctgcac gtgcatgaag
      841 tccgtggcgg tgagccccgt catctgaagg aagccgcggt ccttgagatg cttgaccacg
      901 tcccggtatg tgtacacctc gggggcgcct cgcctgatct cctccagggt gcagagcgga
      961 ttggccttga cgaactcgta cacctccctc tccttggggg agagcttcgg cgtccccctc
     1021 tccacgggaa ggtagtagtt ggtctcaccg gtcgcgccca tcctctcctt cagccaggga
     1081 tgggccgccg aggccacgga caacggcacc acccgttctg gcccgatctt gaagttgccc
     1141 ctctggtcgg gaacgacatg cgagtccccg ccca
//
LOCUS       DAOSYN010000366         1171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_87652_length_1171_cov_3.063620, whole genome shotgun sequence.
ACCESSION   DAOSYN010000366 DAOSYN010000000
VERSION     DAOSYN010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87652_length_1171_cov_3.063620"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PLJ11_08850"
     CDS             <1..991
                     /locus_tag="PLJ11_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO04814.1"
                     /translation="MAPTTMIGQRTATTPFGRDPVKEGYPLKVCEVLDQLQAPVFIER
                     VSVADTKRIMQARRAVRKALEVQRDGKGYAFVEFLSPCPTNWHMDPVTSAQFVTEQME
                     KVFPLGNLRDRSAEPAPVPISIPRATVPQLMEVGREGVSAKTDPSFKERRLKFSGFGG
                     QGVLSLGLVVAEAAGAAGRFVSWLPSYGPEQRGGYASCSVVISGKEVGSPAVDAPDVL
                     VAMSQQALDRFASTVVDGGTVVYEASLSAPKVREGVRTIAFPASRIAAESGTPKAANT
                     ALLGALTALGLHGVPEDKVLEALDASFSAKPALVQRNRKVYEAAYGWAKGNVR"
BASE COUNT          195 a          362 c          419 g          195 t
ORIGIN      
        1 gatggccccg accacgatga tcgggcagag gaccgcgacc acgcccttcg gccgggaccc
       61 ggttaaggag ggttaccccc tgaaggtatg cgaggtgctc gatcagctgc aggcccccgt
      121 gttcatcgag cgcgtgtccg tggccgacac caagcgcata atgcaggcga ggagggcggt
      181 ccgcaaggcg ctcgaggtcc agagggacgg caaggggtac gccttcgtgg agttcctttc
      241 cccctgcccc acgaactggc atatggaccc cgtcacctcg gcgcagttcg tcaccgagca
      301 gatggagaag gtgttcccgc tgggcaatct cagggacagg agcgccgagc cggcgccggt
      361 gccgatctct atcccgaggg cgacggtccc acagttgatg gaagtggggc gggagggcgt
      421 ctcggcaaag accgacccat cgttcaagga gcggcggctc aagttctccg ggttcggcgg
      481 ccagggggtc ctctccctgg ggctggtggt cgccgaggcg gccggcgcgg ccggacgctt
      541 cgtctcctgg ctcccgagct acggcccgga gcagcgggga ggctacgcct cctgctcggt
      601 ggtcatcagc ggaaaggagg tcggatcccc cgctgtggac gcccccgatg tgctggtggc
      661 catgagccag caggctttgg acaggttcgc ttcgaccgtg gtcgacggcg ggacggtggt
      721 ctacgaggcg tccctgtcgg ccccgaaggt ccgggagggc gttcggacca tagccttccc
      781 cgcctcgcgc atagccgcgg agagcgggac gcccaaggcg gccaacacgg ccctgctcgg
      841 ggcgctcacc gccctgggcc tgcacggggt gccggaggat aaggtgctgg aggcgttgga
      901 cgccagcttc tccgccaagc ccgccctggt gcagcggaac cggaaggtct atgaggcggc
      961 ctatgggtgg gcgaagggga acgttcgtta gcgcccctcc ctttcattta tccgatcgta
     1021 atcggtaagc ttcttaagcg cccgcctgtt taggtatggc cattacgaac ccgagcgtta
     1081 gggctgaacg gaggattgcc attgaccaat tctgacaaac ttgggacgcg catacggacg
     1141 taccgcgaac ggttgggcat gagcgtggag g
//
LOCUS       DAOSYN010000367         1161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_88702_length_1161_cov_3.494575, whole genome shotgun sequence.
ACCESSION   DAOSYN010000367 DAOSYN010000000
VERSION     DAOSYN010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88702_length_1161_cov_3.494575"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..345)
                     /locus_tag="PLJ11_08855"
     CDS             complement(1..345)
                     /locus_tag="PLJ11_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04815.1"
                     /translation="MAYRPNRNKSRARKPNNYRNRDAPSTRVGMLNQRRVGEFKYDVR
                     ELFVENKLDPDQASTFLASVIAKASRQSIKDAKDYVREQQATGAISKEIMDGLVYYLD
                     RYTKYQSQQTGD"
     gene            complement(460..924)
                     /locus_tag="PLJ11_08860"
     CDS             complement(460..924)
                     /locus_tag="PLJ11_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOO04816.1"
                     /translation="MNDCRKRAEMAVDELGEQMGGYIPPTLHHIKENHPALAVIIKDM
                     DKVINTDGALDRKTKRLIALACVAVRMCDGCIYPQAKVAKNCGATREEIMEALNIAVL
                     TGGVPAWSTAREGIAQLFEEWDAEDREKEGAKAVPRKVSVKPGGRKGAPKKK"
BASE COUNT          178 a          363 c          339 g          281 t
ORIGIN      
        1 tcaatcgccg gtctgctggc tctggtactt ggtgtaccgg tccaaataat agacgaggcc
       61 gtccatgatc tccttggaga tggcgccggt ggcctgctgc tcgcggacgt agtccttggc
      121 gtccttgatg ctctggcgcg acgctttggc gatcacgctt gccaggaagg tcgatgcttg
      181 gtcggggtcc agcttattct ccacgaacag ttcccggacg tcgtacttga actcgcccac
      241 ccggcgctgg ttcagcatgc cgacccgggt cgagggtgcg tccctgttcc tgtagttgtt
      301 cggtttcctc gccctgctct tgttcctgtt gggtctgtag gccaaagtga tgtctccggc
      361 ataaagtgat gagcatacaa tataagaact tttctaaaat aataataagt aaaacaaaat
      421 ataaaagatg aaatgaccgg gcggacgatc ccgcctggct cacttcttct tgggcgcgcc
      481 cttgcgcccc ccgggcttta cggagacctt cctcggaacg gccttcgcgc cttccttctc
      541 cctgtcctcc gcgtcccatt cctcgaacag ctgcgcgatg ccctccctgg cggtggacca
      601 ggccggcacg ccgccggtca gcaccgcgat gttgagcgcc tccattatct cctcgcgcgt
      661 cgcgccgcag ttcttggcca ccttcgcctg cggatatatg cagccgtcgc acatgcgcac
      721 cgccacgcac gccagggcga tcagccgctt ggtcttcctg tccaacgcgc cgtcggtgtt
      781 gatgaccttg tccatgtcct tgatgatgac cgccagcgcg gggtggttct ccttgatgtg
      841 atgcagcgtc ggggggatgt accctcccat ctgctcgccc agctcgtcca cggccatctc
      901 ggccctcttc ctgcagtcgt tcattgggaa tgcaatgcgg ctgccgactt attatcctta
      961 tggcctatgg gggatggaag cggttcgggg accgtccagg tgctttctca tcgtcacttc
     1021 aggtccagct cggtgcgggg gttgggatgc tcgaatatcc cgcggggcgg ccgttcgctg
     1081 gccccggtcc tgagcttggg gtccaggcgg tcgccggtca ccatgtggta gacgtattcc
     1141 ccaatgttgc aggcgtggtc g
//
LOCUS       DAOSYN010000368         1153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_89592_length_1153_cov_2.831512, whole genome shotgun sequence.
ACCESSION   DAOSYN010000368 DAOSYN010000000
VERSION     DAOSYN010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89592_length_1153_cov_2.831512"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1057
                     /locus_tag="PLJ11_08865"
     CDS             35..1057
                     /locus_tag="PLJ11_08865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtaA/CmuA family methyltransferase"
                     /protein_id="HOO04817.1"
                     /translation="MNLPEKLTPRERVLDALKGKKHDRPPVAVFTQSATMGQMDALGV
                     SWPDAHYNADLMVKLGAGQAKVFGFEAVRAPFCLTVEAETLGMTVDKGRKDRTPMIKG
                     HSFQMEDEPNLMDVKTFLKSGRAAICQEAIRKMKATYGAEYPVIAGNTGPFTLAGHMV
                     GTENLVLYIMVDPDLVHKWVKAVNVICTGYSQALADAGGDVVQMSEPSASTDLLSPEM
                     FDEYAGAYVKGSLAPVKGATGVLHICGNTTPILNNMINTGVKALSIEEKVVPEEAVKI
                     VNHRAALIGNVGVVNPLLQGTPADVMAHGKRCAAAGFDVVSPGCGLSALISNDNLAAL
                     VKAVKG"
BASE COUNT          246 a          349 c          348 g          210 t
ORIGIN      
        1 ggacaggtca gattcctact ggactatagg gggaatgaat ttgcctgaaa aattgactcc
       61 aagggaaaga gtattggacg cccttaaggg caagaagcat gacaggccgc cggtcgcggt
      121 tttcacccag agcgcaacca tgggacagat ggacgccctc ggcgtctcct ggccggatgc
      181 ccattacaac gcggacctta tggtcaagct gggcgctggc caggccaagg tcttcgggtt
      241 cgaggccgtt cgggcgccgt tctgcctgac ggttgaggcc gagaccctgg gaatgaccgt
      301 tgacaagggc cgcaaggaca ggacgcctat gataaagggg cactccttcc agatggagga
      361 cgagcccaac ctcatggacg tcaagacctt cctgaagagc ggccgggctg ccatatgcca
      421 ggaggccata aggaagatga aggcgaccta tggggccgag taccctgtga tcgcgggcaa
      481 caccggcccg ttcaccctgg cggggcacat ggtcggcacc gagaacctcg tgctgtacat
      541 catggtggac ccggacctgg tgcacaagtg ggttaaggcc gtcaacgtga tatgcactgg
      601 atacagccag gctctggccg atgccggagg ggacgtggtc cagatgtccg agccctccgc
      661 aagcaccgac ctgctttcac cggagatgtt cgatgagtac gctggcgcct acgtgaaagg
      721 gtccctggcc cccgtaaaag gggcgacagg tgtactacac atctgcggga ataccacccc
      781 catcctcaac aacatgatca acaccggcgt gaaggccctc tccatcgagg agaaggtcgt
      841 tcccgaggag gcggtcaaga tcgtcaacca ccgggcagcc ctgataggga acgttggcgt
      901 cgtcaacccg ctgctccaag gaaccccggc ggacgtcatg gcccatggca agcgctgcgc
      961 tgccgccggc ttcgacgtgg tgtccccggg atgcgggctg tccgccctta tctcgaacga
     1021 caacctggcc gcgctggtca aggccgtcaa gggctaagcg ccctcaaacc ttttttttcc
     1081 attcctttct ctcgccctac catcctcgtc atagtatctc ttcttgaacc aaagcgccag
     1141 gttcaccagg ctt
//
LOCUS       DAOSYN010000369         1152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_89714_length_1152_cov_2.589790, whole genome shotgun sequence.
ACCESSION   DAOSYN010000369 DAOSYN010000000
VERSION     DAOSYN010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89714_length_1152_cov_2.589790"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(461..>1152)
                     /locus_tag="PLJ11_08870"
     CDS             complement(461..>1152)
                     /locus_tag="PLJ11_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOO04818.1"
                     /translation="SVCLWSDLVRMTKRFEFAFENAGYMKDGRAFTIKVAPTYDRGNG
                     PRNCTLGSVLQKHVDARYIIPPEWIGDETERGTEAFKRTWRYCKGAKSEARVHQDGHR
                     YSYNEGGIPFPDRLDQPSRTILTSEGNRRPNRIGHIIPVDQANNMYRVLTPIELERLN
                     CFEDDWTIGMPERWRYFCMGNALVVGLIEQMGNRLSEVYSMAEEQGSGSIEIRRAAGR
                     IIGPLDMCVQA"
BASE COUNT          277 a          334 c          247 g          294 t
ORIGIN      
        1 taggaaggac ccacggggac acattccaat agttggatgg tagaacgttc tgaccaacga
       61 tcccttccac aaaaagcttc ctgaatagaa cattagaggt atccaaaacg tcaaacaccc
      121 ctgtcatttg aatacagacg ccatccccaa tagtgctcat ggcaccccat catcgaaata
      181 aaggccctca gctgaacggt ccaattagtt cccaattagg tcttgtcgac cctaatgctt
      241 gggactgacc aagaatgttg cgatgcggga ccatatgcaa cctagcgaaa ccccctctat
      301 aatggaatgg acccgccacg tcgtccaaac ggtcaggtca caaaccaatc aatatcgttg
      361 aatgggtgac cataggcaca tgggctttat tgatagaccg gccttcaata tctttgtcat
      421 agctatcatt gacgactttg acaggcaatc gattggatgg ttaggcttgg acgcacatgt
      481 ccaacggtcc aatgatgcgc ccggccgccc ttctgatctc gatgcttccc gaaccctgtt
      541 cctcggccat tgaatagacc tcgcttaacc tgttccccat ctgttcgatc agacctacga
      601 ccaaggcatt gcccatacag aagtacctcc atctctctgg cattccaatg gtccaatcgt
      661 cctcgaagca gttcaatctc tccaattcaa tcggggtgag gactcggtac atgttgttcg
      721 cctggtccac agggataatg tgcccgatcc ggttcggtct acggttccct tcgcttgtta
      781 gaattgttct cgaaggttgg tccaacctat ccgggaacgg tatgccaccc tcattatagc
      841 tatagcgatg cccatcctga tggacccttg cttcgctctt agccccttta cagtacctcc
      901 acgttctctt gaaagcttcg gttcccctct ccgtctcatc accgatccat tccggaggga
      961 ttatataacg ggcatcgaca tgtttttgaa gaacgctacc caacgtacag ttcctgggtc
     1021 cattccctct gtcatatgtg ggggcgacct ttattgtgaa cgctctacca tctttcatat
     1081 agccagcgtt ctcgaatgcg aactcgaacc gcttggtcat cctcacaagg tctgaccaca
     1141 ggcagaccga gg
//
LOCUS       DAOSYN010000370         1142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_90825_length_1142_cov_4.061638, whole genome shotgun sequence.
ACCESSION   DAOSYN010000370 DAOSYN010000000
VERSION     DAOSYN010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90825_length_1142_cov_4.061638"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1007
                     /locus_tag="PLJ11_08875"
     CDS             162..1007
                     /locus_tag="PLJ11_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOO04819.1"
                     /translation="MPRVALYTRVSTEDQAKEGYSLSAQMERLEAYCDAQGWDIAERY
                     VDDGHSGRSTRRPAYRRMMEERDRWDIILVMKMDRIHRNSKNFMGMMEDLERWGKKFS
                     SMSESLDTSNAVGRFVVDIIQRIAQLESEQIGERTYMGMRQKAESGQGPLGFRVPFGY
                     RLQDGKLLQEGSEAATVRFIYDRYLSGRSMGMIAWELNGMDVTTGTGGQWTVWSISRI
                     LHNPVYAGFLRWDDVVVPSGHEAVVSVETFNEVQSRVAARTKNPRHSKAQLLPLDGKL
                     ALCGQ"
BASE COUNT          263 a          288 c          371 g          220 t
ORIGIN      
        1 cggcaaactt gtctgggatg gcatcgagca ggaggccccc cacctgaagg tcatcgagcc
       61 cgccctcttc gaggaggtcc aggggctgat cgctctccgg atgaagtcca ggcggaagca
      121 gaggcggata ccggccaaga ccctcttccg gagcgaggcc catgcctaga gtggccctat
      181 acacccgggt atcgacagag gaccaggcca aggaaggcta ttccctgtcg gctcagatgg
      241 aaagactgga ggcatattgc gatgcccagg gatgggacat cgcggaacgg tatgtggacg
      301 atgggcattc cggtcgcagc accagaaggc ctgcgtaccg gcggatgatg gaggaacggg
      361 acagatggga catcatcctg gtcatgaaga tggaccgcat ccaccgcaac tcgaagaact
      421 tcatgggcat gatggaggac ctggaaaggt ggggaaagaa gttcagttcc atgagcgagt
      481 ccttggacac ctccaatgcg gtgggcaggt tcgtagtcga cataatccaa aggatagcgc
      541 aattggagag cgagcagata ggcgagcgca cctacatggg gatgaggcag aaggccgagt
      601 ccgggcaggg gccgctgggc ttcagggtcc ccttcggata caggcttcag gacggcaagc
      661 tgctccagga gggatcggaa gcggcgacgg tgcgcttcat ctatgatcga tatctttccg
      721 ggcgatcgat ggggatgatc gcctgggaat tgaacgggat ggatgtgacc acaggcacgg
      781 gcggccagtg gacggtatgg tccatcagtc gcatcctgca caaccctgtg tacgccggct
      841 tcctacggtg ggatgatgtg gtggtgccct cggggcatga ggccgtggta agtgtggaaa
      901 cattcaatga ggttcagtca agggttgcgg caaggacgaa gaaccccagg cattcaaagg
      961 cgcaattgtt gccgctggat gggaaacttg ctctctgcgg ccaatgaacg ttagtctgaa
     1021 acctgttcat tgccccctcc ctttacggac catatacggt tttcattgct acaggaatat
     1081 ttggatggtc ttcataccct tcgcaccgat atggctatgt tccgatggca gaacataaca
     1141 tc
//
LOCUS       DAOSYN010000371         1142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_90830_length_1142_cov_3.528059, whole genome shotgun sequence.
ACCESSION   DAOSYN010000371 DAOSYN010000000
VERSION     DAOSYN010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90830_length_1142_cov_3.528059"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLJ11_08880"
     CDS             complement(<1..935)
                     /locus_tag="PLJ11_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO04820.1"
                     /translation="MDQVAPDRRSFETVFKDATRHEPYPYQSRVAQGDALPDLIEATT
                     GMGKTECAVLAWVWRRRYADQTIRDSTSRRLVYCLPMRALVEQTQTKVTLWLERLGLL
                     ETSPTPGPGIRVVKVLGGEVWKDWDTNVEEDQIIIGTQDMLLSRALNRGYAMSRYRWP
                     LHFGLLNNDCTWVIDEPQLMGVGLHTTAQLCWLRKALGTYGPTHTLWMSATIKPDWLR
                     TIDFPAGSVLKAAGLDATDESSEAVAKRLHARKHLAPARAPMDDPKGLAAEITEFHDG
                     HLTLVVVNTVRRAKELYKALASKKGPEVVLLHSQFR"
BASE COUNT          211 a          355 c          327 g          249 t
ORIGIN      
        1 cggaactggg agtgcagaag caccacctcc ggtcctttct tggacgctag cgctttgtag
       61 agttctttag ccctcctcac cgtgttgacc accaccaggg tgaggtggcc atcgtggaac
      121 tccgtgatct cggccgccag tcccttaggg tcgtccatgg gcgcgcgggc gggtgccaga
      181 tgcttcctgg cgtgcaagcg cttggccacg gcctcggagc tttcatccgt cgcgtcgagc
      241 cctgccgcct tgaggaccga gcccgcgggg aagtcgatgg tcctgagcca atcaggtttt
      301 atcgttgcgc tcatccacag ggtgtgcgtg ggaccgtatg tcccaagggc cttccttagc
      361 cagcagagct gggcggtggt atggaggcct acccccatca actgaggctc gtctatcacc
      421 caggtgcaat cgttgttgag gaggccgaaa tgcaggggcc agcgatatct ggacatggca
      481 tacccccggt tcagggccct tgagagaagc atatcctgcg tgccaatgat gatctggtcc
      541 tcctccacgt tggtgtccca gtccttccag acctcccccc cgaggacctt caccacgcgg
      601 atgcccggcc ccggggtggg ggaggtctcc aataggccca accgctccag ccagagggtg
      661 accttcgttt gggtctgctc gaccagggcc ctcatgggaa ggcagtagac aaggcggcgg
      721 gacgtcgagt cccgtatcgt ctggtctgca tatctccgtc gccacaccca tgcgagaacg
      781 gcgcactcgg tcttccccat gcccgtcgtg gcctcgatca ggtctggaag ggcgtccccc
      841 tgcgccaccc tgctctggta gggatagggc tcgtgccttg tagcatcctt aaaaacggtc
      901 tcgaacgatc tgcgatctgg ggcaacttgg tccatctcga ctccctttga cgaattccgt
      961 tcgagactat aatttttatt gcaaacggtt tatttattta taatactaaa taaaataatt
     1021 gcatgcacaa aaaaatgctg agctaggcca tgcgccgccc tgcgcctcat ccctgctcga
     1081 aatggaacgc ccccgttccc tccaagggca cccctcttgg tcaagaggat gggccgatga
     1141 cc
//
LOCUS       DAOSYN010000372         1141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_90937_length_1141_cov_4.601289, whole genome shotgun sequence.
ACCESSION   DAOSYN010000372 DAOSYN010000000
VERSION     DAOSYN010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90937_length_1141_cov_4.601289"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLJ11_08885"
     CDS             <1..766
                     /locus_tag="PLJ11_08885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOO04821.1"
                     /translation="RKRRMWDALVKMGFTEIDDEYVQPAFWNMDALFTPQDHPARDLQ
                     DTFYLKSPSRLPLEDEEIVQRVKAVHEDGGDTGSLGWRYAWSREEAEKALLRTHTTVN
                     TVKYLAKHPDPPAKVFSISRIFRNESIDMTHLPEFMQIEGIIMEEGASFDMLVSWLKE
                     FYRRMGFDKVRVRPGPFPYTEPSLEVDILYNGSWMELGGAGVFRPEVTGPLGVRHPVL
                     AWGLGFERLAMLRWNLKDIRELYITDMELLRQSPIF"
     gene            complement(744..>1141)
                     /locus_tag="PLJ11_08890"
     CDS             complement(744..>1141)
                     /locus_tag="PLJ11_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO04822.1"
                     /translation="LIDGKAQPALDLLLEVRKARSDPLLDCDIAKCYSLLGQKEEALQ
                     MVEAIGRRGGIEDPDVLEEVYHRKGQVLASLGRYQEAIANLSKAIGLARPGDKREIFK
                     AMAACYEALGMDEKAREYNRKAVRRSGSA"
BASE COUNT          203 a          373 c          347 g          218 t
ORIGIN      
        1 gcgcaagcgg cggatgtggg acgccctggt gaagatgggg ttcaccgaga tcgacgacga
       61 atatgttcag cccgcgttct ggaacatgga cgcgctgttc accccccagg accatcctgc
      121 ccgggacctg caggacacct tctacctcaa gagccccagc cggctgccgt tggaggatga
      181 ggagatcgtc caacgggtca aggccgtgca cgaggacggg ggggacaccg gctcgctcgg
      241 ctggcggtac gcgtggtccc gcgaggaggc ggaaaaggct cttcttcgca cacatacgac
      301 cgttaatacc gtaaagtacc tcgccaagca ccccgaccct cctgcgaagg tgttctcgat
      361 ctcccgcatc ttccggaacg agagcatcga tatgacccat ctgcccgagt tcatgcagat
      421 cgagggcatc atcatggagg agggggccag ctttgacatg ctggtcagct ggctgaagga
      481 gttctaccgc cgcatggggt tcgacaaggt gcgcgtccgc ccgggcccat tcccttacac
      541 cgagccgtcc ctcgaggtcg acatcctcta caacggctcg tggatggagc tgggcggggc
      601 gggcgtcttc cgccccgagg tcacaggccc gctgggcgta aggcaccctg tgctggcctg
      661 gggcctgggg ttcgaacggc tcgcgatgct cagatggaat ctgaaggaca tccgcgagct
      721 ctacatcacc gatatggagc tgctcaggca gagcccgatc ttctgaccgc cttgcgattg
      781 tactcccggg ccttctcgtc catccccagg gcctcgtagc aggctgccat cgccttgaat
      841 atctcccgct tgtccccagg cctggccagt cctatggcct tgctcaggtt ggctatggcc
      901 tcctggtacc tgcccaagga ggcgagcacc tggcctttgc gatggtacac ctcctccagc
      961 acgtcagggt cctcgatgcc gcccctccgg cctatggcct cgaccatttg gagggcctcc
     1021 tccttctgcc ccaggagact gtagcacttg gcgatgtcgc aatcgagcaa cgggtcggac
     1081 ctggccttcc taacctccag tagaaggtcc aacgcgggct gggccttgcc atcgatcaga
     1141 a
//
LOCUS       DAOSYN010000373         1138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_91317_length_1138_cov_2.677747, whole genome shotgun sequence.
ACCESSION   DAOSYN010000373 DAOSYN010000000
VERSION     DAOSYN010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91317_length_1138_cov_2.677747"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..748)
                     /locus_tag="PLJ11_08895"
     CDS             complement(200..748)
                     /locus_tag="PLJ11_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO04823.1"
                     /translation="MVDIDLDGMLKGLRSTDVEECRAALEDGRELLTEGWADRWLLQA
                     LIPLTAAEDDQVRRDASWCIGKLALLKLGDPRSVERLIALTADQDEEVRSNAAWALGE
                     LAGLRIGDTASIEALNALLTDEDGEVRGMAAWALGRMAERMRITSPASIPLLQAMRRE
                     GSEYLRKGAEWSLERIERIRAR"
     gene            complement(777..965)
                     /locus_tag="PLJ11_08900"
     CDS             complement(777..965)
                     /locus_tag="PLJ11_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04824.1"
                     /translation="MAEEQGERPACLWHAPDGRCANCKARRFRLDALGMKDTYGECRG
                     QMDYGTCEFYKARPVPKG"
BASE COUNT          178 a          403 c          345 g          212 t
ORIGIN      
        1 gaagtacggc gccgtgaaga gctcagggag acagaccacc ttggcgcccc gccgtgcggc
       61 ctcctccgtc atcctgaccg ccttccgcag gttgtccgac ggatcggagg ccatggccat
      121 ctggaccagc cccactctca gcttgctctc ggagggcata ccctagcgga ttaggggcgc
      181 tatgatttaa ccattcccct cagcgcgccc ggatcctctc gatccgctcc aaggaccatt
      241 ccgccccctt gcgcaggtat tcgctcccct ccctgcgcat ggcctggagg aggggaatgg
      301 aggcggggga ggtgatccgc atcctctcgg ccatccgccc cagggcccag gcggccatcc
      361 cccggacctc cccgtcctcg tcggtgagga gggcgttgag ggcctcgatg gaggcggtat
      421 cccctatcct cagcccggcc agctccccca gggcccaggc ggcgttggac ctgacctcct
      481 cgtcctgatc ggcggtcagg gcgatcagcc gctccacgga gcgggggtcc cccagcttga
      541 gcagggccag cttgccgatg caccaggagg cgtcccggcg gacctggtca tcctcggcgg
      601 cggtcaaagg tatcagcgcc tgcagcagcc agcggtcggc ccatccttcc gtgaggagtt
      661 cgcggccgtc ctcgagggca gcccggcact cctcgacgtc ggtcgagcgc aggcctttga
      721 gcatgccatc caggtcgatg tccaccattt cccgcccctc cacgccggtc agcctatcat
      781 cctttgggca ccggtctggc cttgtagaac tcgcaggtac cgtagtccat ctggcctctg
      841 cattccccat acgtgtcctt catgcccagc gcatcaaggc ggaagcgtcg ggccttgcag
      901 ttcgcgcatc tgccatccgg tgcatgccat aagcatgccg gccgctcgcc ctgctcctcg
      961 gccatggtac tccaggataa tatcgttcgt atatttacga ataatccgct tcgaggtata
     1021 tttaaatgcc cttcggcccc gccttgatca gggtgcgcag gggcaggcat cgctcgctgg
     1081 cccatagggg cgcctggaaa agggaggtgg gcccagccgg attcgaaccg gcggccta
//
LOCUS       DAOSYN010000374         1131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_92063_length_1131_cov_2.579926, whole genome shotgun sequence.
ACCESSION   DAOSYN010000374 DAOSYN010000000
VERSION     DAOSYN010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92063_length_1131_cov_2.579926"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..225
                     /locus_tag="PLJ11_08905"
     CDS             46..225
                     /locus_tag="PLJ11_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04825.1"
                     /translation="MGVAADRQEGDLRCNGMAIDAAERILANGDRAFRWVATDVLREL
                     RGEAVQQRLKSRQKR"
     gene            232..909
                     /locus_tag="PLJ11_08910"
     CDS             232..909
                     /locus_tag="PLJ11_08910"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOO04826.1"
                     /translation="MTEIEYLSHSAFAIRNDGKEVLIDPFLSDNLKAPASALEVRPDL
                     ILVTHAHHDHLGDAVDLSKRFGCPVLSVVEVSDRLEELGAKVVPGNIGGETAFPFCRV
                     KVYPAVHTSSFDDGRYGGAPCSFLVRMGGKAVYHAGDTALFGDLALVGEEAEIDAALL
                     PVGGVYTMGIRDAVKAMRLLGAKRMVPMHYDTWDVISVAPDEMRRASAGEGFELCILA
                     PGERLRL"
     gene            951..>1131
                     /locus_tag="PLJ11_08915"
     CDS             951..>1131
                     /locus_tag="PLJ11_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04827.1"
                     /translation="MAGRGSISCLALCAALLVLSLCPISSASSGDVSIVQVNDGRTIE
                     AGGSASFAWVVHNEGP"
BASE COUNT          206 a          303 c          406 g          216 t
ORIGIN      
        1 cagggagagc catgatgatc gcgatttcgt gcgcaaggcg gtgaattggg cgttgcggca
       61 gatcgacaag agggggacct gcgttgcaac gggatggcca tcgacgcggc cgagaggatc
      121 ctggccaatg gcgaccgggc cttccggtgg gtggctacgg acgtcctaag ggagctgaga
      181 ggcgaggcgg tgcagcaaag attgaagagc cgtcagaaga gatgagattc catgacggag
      241 attgaatatc tgagccactc ggccttcgcc atacgtaacg acggtaagga ggtcctgatc
      301 gaccccttcc tatcggacaa tctgaaagct ccagcctcgg ccttggaggt gcggccggac
      361 ctgatactgg tcacgcatgc ccaccatgac cacctcgggg atgccgtcga cctctccaag
      421 cgctttggat gccccgtgct ttccgtggta gaggtgagcg atcgccttga ggagctcggg
      481 gcgaaggtgg tgccgggcaa catcggaggg gagaccgcct tccccttctg tagggtgaag
      541 gtctacccgg cggtccatac gtcatcgttc gatgatgggc ggtacggagg ggcgccgtgc
      601 tcgttccttg tgcggatggg agggaaggcc gtgtaccatg caggggacac cgcgctcttc
      661 ggcgatctcg ccttggtggg ggaggaggcc gaaatagacg cggcgctgct tcccgtggga
      721 ggggtgtata ccatgggcat ccgcgacgcc gtcaaggcaa tgcgcctgct cggcgccaag
      781 cggatggtgc ccatgcatta cgatacttgg gatgtgatct cggtcgctcc cgatgagatg
      841 cgcagggcga gcgcggggga ggggttcgag ctgtgcatct tggcccccgg ggagcgcttg
      901 aggctgtgaa gcaaaggttt tatccttctt ggccatgaag ggatatggcc atggcgggaa
      961 ggggaagcat ttcgtgcctg gcgctgtgtg cggcgctcct cgtgctcagc ctctgcccta
     1021 tctcctcggc ctcctcgggc gatgtttcca tcgtgcaagt gaacgacgga cggaccatcg
     1081 aagcgggagg aagcgcgtcg ttcgcatggg tggtccacaa cgagggccca g
//
LOCUS       DAOSYN010000375         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_92152_length_1130_cov_3.880930, whole genome shotgun sequence.
ACCESSION   DAOSYN010000375 DAOSYN010000000
VERSION     DAOSYN010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92152_length_1130_cov_3.880930"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..>1130
                     /locus_tag="PLJ11_08920"
     CDS             126..>1130
                     /locus_tag="PLJ11_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04828.1"
                     /translation="MAVVAVVAILLIASLAVLFWPDGGGDEGSATIGTQGGVLSIGSG
                     ALKGLRIEVPTGATSTPVDFKVTSREVDTLAGLPDIGSIVSRLITIGTDGTDDWNTFK
                     IFDKTLTVTLPYDEDLVDDERAVRFYQYDAENEHLEPVGFLSQDYEADTITFNAATFS
                     QFVAVELAMSIFEGMNVSFAVDTGFRPKTDGWFIPNYGSYLESGGLCLGMTSYAKWYY
                     TYQKGDGPGLYNSYRQGDRDEWRDDATAIELATRVQLGVQGIWGAMTAEERRNCSSVQ
                     TGMSIIHGMLVSGEPQLVGLKTFYVDGTWASGGHAILAYKYDNGRFYVYDPNNPGSEP
                     S"
BASE COUNT          242 a          315 c          352 g          221 t
ORIGIN      
        1 atttcgctgg acgcgggagc gtccagcatt aacgcaataa tcaactctcc ctaatttcga
       61 tctgaccaag tgtcgaagtc aggtactacc agcctcccaa gcggaagcgg aacactaagc
      121 tcatagtggc ggtggtcgcc gtcgtggcga tcctgctcat cgcctcctta gcggtgctct
      181 tttggcctga tggagggggc gacgagggga gtgcgaccat tggcacccag ggcggagtgc
      241 tctcaattgg cagcggtgct ttgaagggac tgcggatcga ggtgcccacg ggcgccacgt
      301 cgactccagt tgatttcaag gtgacgagca gggaggtgga caccctggca ggccttccgg
      361 acatcggaag cattgttagc cgtctcataa ccataggcac tgacggcacc gatgattgga
      421 acacttttaa gatattcgac aagaccctga ccgtcaccct gccctacgac gaggacctag
      481 tggatgatga gagggcggtg cgcttctatc agtatgatgc tgagaacgag catctggagc
      541 cggtcggctt tttgtcccag gactacgaag ccgataccat tacgttcaac gccgccacct
      601 tctctcagtt cgtggccgtg gagctggcca tgagcatatt tgaagggatg aacgtgagct
      661 tcgcggtgga cacgggcttc cgcccgaaga ccgacgggtg gttcataccc aactacggca
      721 gttacctgga gagcgggggc ctctgccttg gcatgacctc ctacgccaag tggtactaca
      781 cataccagaa gggcgacggc cccggtctat ataacagcta taggcagggg gacagggatg
      841 aatggaggga cgatgccacg gccatcgagc tcgccacaag ggtgcagctg ggcgtccagg
      901 gcatctgggg ggcgatgacc gccgaggagc ggagaaactg ttcctccgtc cagaccggca
      961 tgagcatcat tcatggaatg ctcgtctccg gcgagccgca gctcgtcggg cttaagacct
     1021 tttacgtcga cggcacttgg gcttcgggag ggcatgccat cctcgcctac aagtacgaca
     1081 atgggcgctt ctacgtgtac gacccgaaca acccggggag cgagccgagt
//
LOCUS       DAOSYN010000376         1123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_92963_length_1123_cov_2.772472, whole genome shotgun sequence.
ACCESSION   DAOSYN010000376 DAOSYN010000000
VERSION     DAOSYN010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92963_length_1123_cov_2.772472"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLJ11_08925"
     CDS             <1..709
                     /locus_tag="PLJ11_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOO04829.1"
                     /translation="QFISTLRADLAEAKANEPMLATSDVLVGGTSAALYDTSVFTARE
                     FTNIEILVVIGIFIVLMIVLGSILLPTFAIISIAMSITWSFALTYLVFGVWLNLPVLW
                     LIPLILFVILMGIGIDYNVFILTRIREEIHKGKETKVAVVDAVDWTGGIITALALIMT
                     GAFGALMLSSNAMLQEFGFALTVAVLLDAMVVRTYIVPAALAVMGEKAWWAPGRLQRE
                     GREEKMRRKAEEKAKRK"
BASE COUNT          212 a          328 c          360 g          223 t
ORIGIN      
        1 gcaattcata agcacattga gggccgacct ggccgaggcc aaggccaacg aaccaatgct
       61 ggcaacctcg gatgttctgg tgggagggac ctccgcggcc ttgtatgaca cgagtgtgtt
      121 caccgccagg gagttcacca acatcgagat tctcgtggtc atcggtatct tcatcgtgct
      181 gatgatcgtg ctcggctcca tcctcctccc gaccttcgcg atcatctcca tcgcgatgag
      241 catcacgtgg tccttcgccc tcacctacct tgtgttcggt gtgtggctga atctgccggt
      301 gctgtggctg atcccgctga tcctgttcgt catcctgatg ggcatcggga tagactacaa
      361 cgtcttcatc ctgacccgca tcagggagga gatccacaag ggcaaggaga ccaaggtggc
      421 ggtggtggac gccgtggact ggacgggcgg catcatcacc gcccttgcct tgataatgac
      481 gggggcgttc ggggccctga tgctctccag caacgccatg ctacaggagt tcggcttcgc
      541 gttgaccgtg gcggtgctcc tcgatgcgat ggtggtgcgc acctacatcg tcccggccgc
      601 gttggcagtg atgggcgaaa aggcctggtg ggcccccggc cgcctgcagc gggaggggcg
      661 cgaggagaag atgcgcagga aggccgagga gaaggcgaag cggaagtgag cgatcgaacg
      721 tggccggccg gccacctttt ccttttacat attctttttt tgagtggacc ggtcaatgtt
      781 cggaacgcgc tcgcctcaag aagggcgtat acctggacag cgatcccatg acgtgcggga
      841 tgcaacctcg acatgggcgt cccgatcacg cccccagtac cggcaatgga gctggcctga
      901 agacggagcg ggcgaacgga ccttccgccg gaagaggctg cgccgccacg gcgtcgcctt
      961 gaacgaggac cgcgttctcg ccgtcggcat gcccgggggt ggggaggggc gaagcaagcc
     1021 ctgagccttg ccaatcgagg ataatgtttt tgtgcccgca gattaaaccc tatccgaggt
     1081 gtgagaagat gcacccatat aacctcctgg tgaccttcca cgg
//
LOCUS       DAOSYN010000377         1112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_94324_length_1112_cov_2.465468, whole genome shotgun sequence.
ACCESSION   DAOSYN010000377 DAOSYN010000000
VERSION     DAOSYN010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94324_length_1112_cov_2.465468"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLJ11_08930"
     CDS             complement(<1..727)
                     /locus_tag="PLJ11_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO04830.1"
                     /translation="MDGAAKRKGRTPAVFAALFLLLFVFMVLDLTQAYDPLTFKEAFW
                     ALLGKGTETHRFIVWEMNLPRVIMACLVGAGLGIAGAVMQAVFRNPMASPYILGLSSG
                     ASLGAAVGLTFTVAWLPQMLMIPLLAFVSCMATLFLVYYISNIGGRVPTETLLLAGIA
                     VGSLLSALVSLLTYLAGEQMQGIVYWTMGNLTNATWENILIVAPLIFAGSLIMVSSSR
                     DLNAMMLGDAHALDLGVEVKRVRL"
     gene            complement(780..>1112)
                     /locus_tag="PLJ11_08935"
     CDS             complement(780..>1112)
                     /locus_tag="PLJ11_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04831.1"
                     /translation="RSAFLQFPDLDVVLLDGYYPGTAEEFKRWIGSDDITVYKLNKSW
                     NSYCPDATEGLWAVACLFYPEYFEGELPVEEGEAADDGWNMMIYGAIGIAVVAVAVAA
                     VLLTRRRD"
BASE COUNT          219 a          359 c          356 g          178 t
ORIGIN      
        1 gcaggcggac ccttttcact tccacaccca ggtcaagggc gtgggcgtcc ccgagcatca
       61 tggcgttcag gtccctggag gaggacacca tgatcaggct gcccgcgaat atgagcgggg
      121 ccacgatgag gatgttctcc caggtggcgt tggtcaggtt gcccatcgtc cagtacacga
      181 tgccctgcat ctgctctccc gccaggtacg tgaggaggga cacgagggcg gagagcaggg
      241 aaccgaccgc gatacccgcc aggagcaacg tttccgtggg caccctcccc ccgatgttgg
      301 agatgtaata gaccaggaac agggtggcca tgcaggacac gaacgccagc agggggatca
      361 tgagcatctg cgggagccag gcgacggtga acgtgaggcc caccgccgcc cccagcgagg
      421 ccccggagga caggcccagg atgtacgggg aggccatggg gttccggaac accgcctgca
      481 tcaccgcccc ggcgatcccc agccctgcgc ccaccaggca ggccatgatc accctgggga
      541 ggttcatctc ccatactatg aagcgatgcg tctcggtgcc cttccctagc agcgcccaga
      601 aagcctcttt gaaggtgagc gggtcgtacg cctgcgtcag gtccaggacc atgaacacga
      661 agaggaggag gaacagcgct gcgaagaccg cgggggtccg tcctttcctc tttgccgcac
      721 cgtccaagcc atctcacctc acggagtgcg ctggaacatg gattggcagg gcaaggagct
      781 caatccctcc ttctcgtgag caggacggcg gccacggcca cggcgaccac cgcgatgccg
      841 attgcaccgt agatcatcat attccatcca tcgtcggccg cctccccttc ctcgacgggg
      901 agttcgccct cgaagtactc ggggtagaac aggcaggcca ccgcccacag gccctcggtc
      961 gcgtccgggc agtaggagtt ccaggacttg ttcagcttgt agacggtgat gtcgtcgctg
     1021 ccgatccagc gcttgaactc ctccgcggtc ccggggtaat agccatcgag cagtacgacg
     1081 tcgaggtccg ggaactggag gaaggccgag cg
//
LOCUS       DAOSYN010000378         1109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_94707_length_1109_cov_2.072106, whole genome shotgun sequence.
ACCESSION   DAOSYN010000378 DAOSYN010000000
VERSION     DAOSYN010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94707_length_1109_cov_2.072106"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /gene="istA"
                     /locus_tag="PLJ11_08940"
     CDS             complement(<1..805)
                     /gene="istA"
                     /locus_tag="PLJ11_08940"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOO04832.1"
                     /translation="MRDAMDWSEMREMRRKGMSITQISRVSGCDRKTVSKYIGAESPP
                     RYVRGPSRSVLDPFKDHIRARLAEHPLSAARLYREIRELGYAGSLTTVKRFTSPIKDA
                     SLVLAEVRFETKPGEQAQVDWIDLGRMPVGGTVEHIYVFVMVLSWSRMRFIRMTTDCR
                     TDTFIDCHLKAFEHFGGWTSSILYDNTKNVVLKRALLSSESTFNPMYLDFARHHGITP
                     RLCRPGIAGAKTKGKVERTIQYVERDFLLGSTFNDIQHANSQGMAWCDRV"
     gene            complement(847..>1109)
                     /locus_tag="PLJ11_08945"
     CDS             complement(847..>1109)
                     /locus_tag="PLJ11_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04833.1"
                     /translation="SKEIERFYPHVPILKAGGNYDWKSYDSKRLFILETKKPRIILST
                     MQTASTPDFLSFIGQASEMALIADEVHRLVSRATEFSSISTI"
BASE COUNT          242 a          294 c          307 g          266 t
ORIGIN      
        1 tgacgcggtc gcaccatgcc atcccttggg agttggcatg ctgaatgtcg ttgaacgttg
       61 aaccgagcag gaagtcccgt tcgacgtact ggatggttcg ttcgaccttc cccttcgtct
      121 tcgcacccgc tatgccggga cggcataggc ggggcgttat gccgtgatgc cgggcgaagt
      181 ccaggtacat ggggttgaag gtcgactctg aggacaggag ggcgcgcttg agcaccacgt
      241 tcttggtgtt gtcatagagg atggatgacg tccatcctcc gaagtgttcg aacgccttca
      301 gatggcagtc gatgaaggtg tcagtgcggc agtcggtggt catgcggatg aaacgcatgc
      361 gggaccagga gagcaccatg acgaagacgt agatatgttc cacagtgccg ccgaccggca
      421 tgcggcccag gtcgatccaa tcgacctggg cctgctcacc cggcttcgtc tcgaaacgta
      481 cctccgccag taccagcgag gcatccttga tgggggaggt gaaacgtttg accgtggtga
      541 ggcttccagc ataccctagt tccctaatct cgcggtaaag cctcgccgcc gagaggggat
      601 gttcggccag acgcgccctg atatgatcct tgaatgggtc gaggacgctt ctgctaggtc
      661 ctcgtacata tcggggcggg gactccgccc cgatgtactt gctgacggtc ttgcgatcac
      721 atccagacac tctggatatc tgcgttatcg acattccttt cctgcgcatc tccctcatct
      781 ccgaccaatc catggcgtcc ctcacttcct ccccctcacg ggggtattaa cagggggtga
      841 ggaaatttaa atggtcgaaa ttgaggaaaa ttcagtggcc cgtgacacta gcctatgcac
      901 ttcgtcagca atcagagcca tctcacttgc ctgaccaatg aaggatagaa aatcaggtgt
      961 ggatgcagtt tgcatcgttg acagaataat tctaggtttt tttgtttcta atataaacaa
     1021 tcttttcgaa tcgtaggatt tccaatcata attcccacca gctttaagaa ttggaacatg
     1081 gggatagaat cgctcaatct ctttagacc
//
LOCUS       DAOSYN010000379         1108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_94783_length_1108_cov_3.045584, whole genome shotgun sequence.
ACCESSION   DAOSYN010000379 DAOSYN010000000
VERSION     DAOSYN010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94783_length_1108_cov_3.045584"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /gene="trpA"
                     /locus_tag="PLJ11_08950"
     CDS             complement(<1..513)
                     /gene="trpA"
                     /locus_tag="PLJ11_08950"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOO04834.1"
                     /translation="MEDAFERARGEGRTALICYLMAGAPDMRRSMEIALACLDGGADV
                     LEVGIPFSDPVADGPVIQEAGAKALEGGTTPLKVLEMVAELRTRTDRPLVLMGYYNPI
                     FRRGEERFVREAVTAGADGLIVPDLPLHEGAGLRAICMREGLPLVQLCTPLTPLVRQR
                     KLMEATSGFLY"
     gene            complement(516..>1108)
                     /locus_tag="PLJ11_08955"
     CDS             complement(516..>1108)
                     /locus_tag="PLJ11_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOO04835.1"
                     /translation="PTMVRDFQSVIGQEIRARLLEAEGRLPDLLVACVGGGSNAIGTF
                     HPFLGDEGVRLLGAEAAGEGLETGHHSASLTRGEVGVLHGCKAYLLQDEYGMIKEAHS
                     VAPGLDYPGVGPEHCLLKDIGRAEYVGVTDRQALDAFVTLSRVEGIIPALESAHAVHA
                     GMAEAARMKRDDIVVITVSGRGDKDLETVFSLGVVP"
BASE COUNT          170 a          437 c          332 g          169 t
ORIGIN      
        1 atacaggaag ccggaggtgg cctccatgag cttgcgctgc cgcaccaggg gcgtcagcgg
       61 ggtgcacagc tgcacgaggg gcagtccctc cctcatacag atggcccgaa ggcccgcgcc
      121 ctcgtggagc ggcaggtccg ggacgatcag gccgtccgcg ccggccgtaa cggcctcgcg
      181 cacgaaccgc tcctccccgc ggcggaagat ggggttgtag tagcccatga ggaccagggg
      241 cctgtcggtg cgcgtgcgca attccgctac catctcgagc accttcagcg gcgtcgtccc
      301 tccttccagg gccttcgctc cggcctcctg gatcaccggg ccgtccgcca cggggtcgga
      361 gaacgggatg cccacctcga ggacgtccgc tcccccgtcc aggcaggcaa gggcaatttc
      421 catcgaccgc cgcatgtccg gagcgccggc catcaggtag catatcaggg ccgtccgccc
      481 ctcccccctc gcccgctcga aggcgtcctc cagcctcagg gcaccacccc cagggagaac
      541 acggtctcca ggtccttgtc cccgcgcccg gagacggtta tgacgacgat atcatccctt
      601 ttcatcctcg ccgcctccgc catccccgcg tgcaccgcgt gcgcgctctc caaggcgggg
      661 atgatgccct ccaccctcga caacgtaacg aacgcatcga gcgcctggcg gtcggtcacc
      721 cccacgtact ccgcgcgccc gatgtccttg agcaggcaat gctcgggccc cacccccggg
      781 tagtcgagcc ccggggcgac gctgtgggcc tccttgatca tcccgtactc gtcctgcagc
      841 aggtaggcct tgcacccgtg gagcaccccc acctcgcccc gggtaaggga ggcggaatga
      901 tgccccgtct caaggccttc ccccgccgcc tccgccccca gcagccgcac gccctcgtcc
      961 cctaggaacg gatggaaggt gcctatggcg ttgctccccc ctcccacgca ggccacgagc
     1021 aggtccggga gccggccctc cgcttccaac aaccgcgccc tgatctcctg gccgatgacg
     1081 ctctgaaagt cccgcaccat ggtggggt
//
LOCUS       DAOSYN010000380         1100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_95858_length_1100_cov_2.104306, whole genome shotgun sequence.
ACCESSION   DAOSYN010000380 DAOSYN010000000
VERSION     DAOSYN010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95858_length_1100_cov_2.104306"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..980
                     /locus_tag="PLJ11_08960"
     CDS             204..980
                     /locus_tag="PLJ11_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04836.1"
                     /translation="MIEEAIGWDGSPATPVKHLIGRCRNGSLLMFHKPGKEAFEGTNE
                     NDMRPMIIIDGKELAPPTFSFIWSDLTNVAMADFEAFRAILLLIYRSAYLLDHQYDVN
                     GNLRYAPSPDIMRCIHALDDLVGHRTEFKSIEGLLRFIDLLGWNEDVKYHSFGRTASF
                     TEGRYANKTKIGRINTFLTCIRIPYEVSRFMREYNDRKGDDATKFVELYDIMQALIAS
                     RGVCTPKRRSDLFDFLSPYLVGTRPEREQLVVGSLQRRIV"
BASE COUNT          282 a          239 c          299 g          280 t
ORIGIN      
        1 gggtggattt aacctattat taccaaatgg ttatcattat gcatggtatt tatgatttat
       61 atgtaattat ttggtaataa tcgatttcac aagcgaccga ttgcaatacc catatggcct
      121 ggactgccgt agattgggag aacgtactaa ggacggagta tatttcgggc actccagcgg
      181 tactccagag aagacggctg cagatgatcg aggaggcgat tggatgggac ggatcgcccg
      241 ccaccccggt caaacatctg atcggtcgat gccgcaatgg gtccctattg atgttccaca
      301 aaccggggaa ggaagcgttt gaaggcacca atgagaacga tatgcgtcct atgatcatca
      361 ttgatggaaa ggagcttgcc ccacctacgt tctcttttat atggagcgac ctcaccaacg
      421 tagcaatggc ggacttcgag gcctttcgcg ccatccttct attgatttat cgaagcgcat
      481 accttttgga ccatcagtat gatgtgaacg gaaatctaag atatgcgcca tcccctgaca
      541 tcatgcgctg catccatgcg ctggatgatc tggtcggcca cagaacagag ttcaagagca
      601 ttgaggggct ccttaggttc atagacctct taggttggaa cgaggacgtg aaatatcatt
      661 ccttcggtcg aaccgcctcc tttacggaag ggaggtatgc aaacaagacg aagattggcc
      721 gaataaatac gttcctgacc tgtataagga taccctatga ggtgtccagg ttcatgcgtg
      781 agtacaatga ccggaaaggg gacgatgcca caaagtttgt ggagctctat gacatcatgc
      841 aggctttgat tgctagccgg ggggtatgca cgccaaagag aaggtcggac ctgtttgatt
      901 ttctgagccc gtaccttgtt gggacaaggc cagaaaggga gcagctggtc gtaggttccc
      961 tccagaggag gatagtatga ggacgttcat tcttaatagc aataacgtta ttgatatggt
     1021 ctgccatcgg gatttggatg cacaagacgg tcatagagct gttcgctggc gtgggcggtt
     1081 ttcatcttgg gctttcgaaa
//
LOCUS       DAOSYN010000381         1096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_96340_length_1096_cov_2.636888, whole genome shotgun sequence.
ACCESSION   DAOSYN010000381 DAOSYN010000000
VERSION     DAOSYN010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96340_length_1096_cov_2.636888"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLJ11_08965"
     CDS             complement(<1..627)
                     /locus_tag="PLJ11_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04837.1"
                     /translation="MRLPLLATRVGILAVKLFVVVLVALSIFPLLMGGIGIDLEDEEE
                     GDWALDGSTIRLEAPLMVSNDGFFDITDLRVGFTFKDKDGNVLAVSEMAPMDIPAGRE
                     TAVPIEIALDLYDLDHQTRSDLIFNGTDIAFDLSVQVRYTWNLVRMSVDGESEMGWDA
                     LLGNLQVRSDMAYLREMDGNHTVVVPFTFYANDMVQGGQALALTEYWDS"
     gene            732..>1096
                     /locus_tag="PLJ11_08970"
     CDS             732..>1096
                     /locus_tag="PLJ11_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOO04838.1"
                     /translation="MSEIRVRKRRRLREKELKALAETLFSVTGAEMLGPKDTIDVAEG
                     PGCELILVNNVIEGMIVDGQPFPTIRGLLRNKATKRYVTVDMGAVPFVTNGADVMAPG
                     IVEVDPEVKEGDLVWIRDMT"
BASE COUNT          225 a          325 c          355 g          191 t
ORIGIN      
        1 ggagtcccaa tattcagtga gcgcaagcgc ttgtcccccc tggaccatgt cattggcata
       61 gaaggtgaag gggaccacca ccgtgtggtt cccgtccatc tccctcaggt acgccatgtc
      121 ggaccggacc tgcaggttcc ccagcagcgc gtcccagccc atctcgctct ccccatcaac
      181 gctcatccgg accaggttcc atgtgtacct gacctgaacg ctgaggtcga aggcaatgtc
      241 ggtgccgttg aagatgaggt cggaccgcgt ctggtggtcg aggtcgtaga ggtcgagggc
      301 gatctcgatg ggaacggccg tctcccggcc ggcggggatg tccatcgggg ccatctcgct
      361 gactgccagc acattgccgt ccttgtcctt gaaggtgaag cccacccgca ggtcggtgat
      421 gtcgaagaag ccgtcgttgc tcaccatcag gggcgcctcc aaacggatgg tgctgccgtc
      481 cagggcccaa tcgccctcct cctcgtcctc gaggtcgatc ccgatcccgc ccatcaaaag
      541 cggaaagatg ctcaatgcca cgaggacgac gacgaacagc tttaccgcca atatccccac
      601 ccgggtggcc aataggggaa gcctcatgta aggacggagg atgttcgatg cttataaaac
      661 aatgcggtgc cagtatcaag acggtcccta aaggcgcaaa gttcattggc gatcggacca
      721 tagggaagga cgtgagcgag atcagggtaa ggaagcgcag gcggctgcgg gagaaggagc
      781 tgaaggcctt ggcggagacg ctgttctccg tgaccggcgc ggagatgctg ggccccaagg
      841 acaccattga cgtcgccgag gggccgggct gcgagcttat cctcgtcaac aacgtgatcg
      901 agggcatgat cgtggacggg cagccgttcc cgaccatccg cgggctgctc aggaacaagg
      961 ccacgaaacg ctatgtcacg gtggacatgg gcgccgtccc gttcgtgacc aacggggcgg
     1021 atgtcatggc cccgggcata gtggaggtgg accccgaggt gaaggagggg gacctcgtct
     1081 ggatccggga catgac
//
LOCUS       DAOSYN010000382         1089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_97222_length_1089_cov_3.080271, whole genome shotgun sequence.
ACCESSION   DAOSYN010000382 DAOSYN010000000
VERSION     DAOSYN010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97222_length_1089_cov_3.080271"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..>1089
                     /locus_tag="PLJ11_08975"
     CDS             38..>1089
                     /locus_tag="PLJ11_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOO04839.1"
                     /translation="MIERGILEWRVAIPEKRGIYHEKGMVFHDTCEHVVFLSGSWNET
                     LSGYQRNAERIDVHLEEWDPIRCEEARRWFDSVWHGEERWLRILPIQEALKRGDVEQR
                     EDDVPISTKRKTCDPGSWTPELLFLAIQDKAPWSLKDPYIGTLVEPLPHQVHVHQRTF
                     SRPPLRFLLADEVGLGKTIEAGLILSTMVATGLARRVLILAPANVLEQWEDELRRKFR
                     LEPWVLKGKSWRRGEEEWRTDDLPFNHLPGDSPHILLVSQGIAFRANRRGEFERSSWD
                     LIIVDEAHRARGNKEGEQYRKNNLLKTLDLLSLNTASILLLTATPMQLGVHELFDLLY
                     LLGLPPRWRRREAFQL"
BASE COUNT          287 a          244 c          306 g          252 t
ORIGIN      
        1 aaatgaatat gaatggtgcg ggcgggtgct cgccacgatg atcgagagag gaatattgga
       61 atggagagtg gccatacccg agaagagagg catctaccat gagaagggaa tggtcttcca
      121 cgatacttgt gaacatgtgg tattcctgag cggaagctgg aatgagaccc tatcaggata
      181 tcagcgcaac gctgagagga tcgatgtaca tctagaggaa tgggacccca taagatgtga
      241 ggaagcccga aggtggtttg atagcgtatg gcatggcgaa gagagatggc taaggatcct
      301 tcctattcag gaggccttaa aaagagggga tgttgaacag agggaggatg atgttcctat
      361 ctccacaaaa agaaagactt gcgatcccgg ttcttggacc ccggaactcc tatttttagc
      421 cattcaggat aaagcacctt ggtcgctgaa ggacccctat ataggtaccc tagttgaacc
      481 tctacctcat caggtccacg tccaccagag gaccttttcc agaccgcccc ttaggttcct
      541 tttggccgat gaggttggtc tggggaagac catagaagct ggtctgatat tatccacaat
      601 ggttgctact ggcctagcac gaagggtgct catcttagcc cctgccaatg tcctagagca
      661 atgggaggat gagttgagga ggaagttccg tttggaacct tgggtcctaa agggaaaaag
      721 ctggagaaga ggagaggagg agtggaggac tgatgatctt ccgttcaatc atcttccagg
      781 tgactcgccg cacattcttc tagtaagcca gggcatcgca tttcgagcca atcggagagg
      841 agaattcgag agaagttcct gggacctgat aatcgtagat gaagcccatc gcgccagagg
      901 taacaaggag ggagaacaat atcgtaagaa caaccttctg aagacccttg atctgttatc
      961 tttgaacacg gcgtccattc tgttactgac cgccacccct atgcagctgg gcgtccatga
     1021 gctattcgac ctcctttatc tcttaggtct ccctcctcgt tggagacggc gggaggcgtt
     1081 ccaattatt
//
LOCUS       DAOSYN010000383         1084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_97906_length_1084_cov_2.348882, whole genome shotgun sequence.
ACCESSION   DAOSYN010000383 DAOSYN010000000
VERSION     DAOSYN010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97906_length_1084_cov_2.348882"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLJ11_08980"
     CDS             <1..187
                     /locus_tag="PLJ11_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04840.1"
                     /translation="QLKVMVLVDEAAGVAIIVIMASSAGNTSATRLVGVMVAPAAAVT
                     KEVKENGVRVSPVLLKR"
     gene            complement(207..494)
                     /locus_tag="PLJ11_08985"
     CDS             complement(207..494)
                     /locus_tag="PLJ11_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04841.1"
                     /translation="MTTPVCTYTIDKQTVVGGHKQVFVTFTFSTYATGGKAFAPSLFG
                     IHDYYGCTGNYAPTKGWYTYNGTTLKVYTAYNTEFSDAGDAGSCQLIIYGK"
     gene            complement(526..>1084)
                     /locus_tag="PLJ11_08990"
     CDS             complement(526..>1084)
                     /locus_tag="PLJ11_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04842.1"
                     /translation="QTTNDLDIYGLNRDAAAAWADAYVSQNGNTDRTFTFSLLDSVFS
                     GVRPYWDNNYYDGKFMLTGFDTLERMQQLGMSQLRFTQQDKVQFTVGDGVKTIKGADV
                     GFDTSSYKGVPIVPDAYATADTISRIFVCDGNNLSMCWLQPIQYVETVDPLIAASHRK
                     WGEYHAQGEVVCTRFKAQGKVRDLK"
BASE COUNT          190 a          301 c          349 g          244 t
ORIGIN      
        1 ccagttgaag gttatggtgc tcgtagacga ggcggcgggg gtggccatca tcgttattat
       61 ggcctcctcc gccgggaaca cgtccgcgac gaggctggtg ggggtgatgg tcgctccagc
      121 cgccgccgtg acgaaggaag taaaggagaa cggggttcgg gtgtcccccg tcttgctaaa
      181 gaggtaggcg aagcgaccca tgtgcctcac ttcccgtaga ttatgagctg gcagctcccc
      241 gcgtccccgg cgtccgagaa ctcggtgttg tacgcggtgt acaccttcag ggtcgtccca
      301 ttgtaggtgt accacccctt cgtcggtgca tagttcccgg tgcatccgta atagtcgtgg
      361 atgccgaaca gggacggggc gaacgccttt cctccggtgg cataggtgct gaaggtgaat
      421 gtcacaaaga cctgcttatg ccccccgacg accgtctgct tatctatcgt gtatgtacat
      481 actggtgtgg tcattttttt acctcatgga aagggaggaa ggggtttact taaggtctcg
      541 gaccttcccc tgcgctttga acctggtgca gacgacctcg ccctgcgcgt ggtactctcc
      601 ccacttgcgg tgagaggcgg cgatgagcgg gtcaacggtc tcgacgtact ggatgggctg
      661 gagccagcac atgctcaggt tgttcccgtc gcacacgaat atcctggata tggtgtcggc
      721 ggtggcatat gcgtcaggca cgatgggcac gcccttgtag ctggaggtgt cgaagccaac
      781 gtccgcgccc ttgatggtct tgaccccgtc cccgacggtg aactgcacct tgtcctgctg
      841 agtgaaccta agctggctca tgccgagctg ctgcatcctc tccagggtgt cgaagccggt
      901 gagcatgaac ttgccgtcgt agtagttgtt gtcccagtac ggccttaccc cggagaacac
      961 ggagtccagc agggagaagg tgaacgtcct gtcagtgttg ccgttctgcg agacataggc
     1021 gtcggcccag gcggctgcgg cgtcacggtt caggccgtag atgtccaggt cgttggtggt
     1081 ctgc
//
LOCUS       DAOSYN010000384         1083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_98103_length_1083_cov_1.704280, whole genome shotgun sequence.
ACCESSION   DAOSYN010000384 DAOSYN010000000
VERSION     DAOSYN010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98103_length_1083_cov_1.704280"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..697)
                     /locus_tag="PLJ11_08995"
     CDS             complement(59..697)
                     /locus_tag="PLJ11_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04843.1"
                     /translation="MNARDMRQYENFRRQLGPVPRPGLMVLDVRTTQFVVVELVLLKA
                     LIGEGNPGLFISVDRPHQYMVHLLDMHGIEHTSVTFIDTVSQFASSDAHRQAKVGFFK
                     GPNNIDTLPSALWEWSSEDHGFDLAQCRFALIDNISNLLTFNSHQKVQRFIREFLDLL
                     GDRAAVLLVIDRQRNPNLYHSVLSMGGTELRPEAEICNGNGEGSFKDSMTDS"
     gene            834..>1083
                     /locus_tag="PLJ11_09000"
     CDS             834..>1083
                     /locus_tag="PLJ11_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04844.1"
                     /translation="MLGGDALGLIMVYAYVGAIAALAAKWRFLRERSLQRKFIHVMVG
                     NIALFWWVFDTRYAMALLAAAPFIPVLLLFSRKAGEGDG"
BASE COUNT          189 a          321 c          319 g          254 t
ORIGIN      
        1 cgggatcctc tgtagcccca cccggtccct ctcattgcct tccatggtca attccctctt
       61 agctgtccgt catgctgtcc ttaaagctac cctctccgtt cccattgcat atctcggcct
      121 caggcctcag ctcggtcccg cccattgaga ggaccgaatg atagaggttc gggttcctct
      181 gcctgtcgat gacaagcagg accgccgccc tgtcccccaa taggtccagg aactccctta
      241 tgaacctttg gaccttctgg tggctattga acgtcagaag gttggatata ttgtcgatga
      301 gcgcgaaccg acattgggcc aggtcgaagc cgtggtcctc cgaggaccac tcccacaggg
      361 cgctgggaag cgtgtctatg ttgttcggcc ccttgaagaa gcctaccttc gcctgccggt
      421 gcgcgtcgga ggacgcgaac tgagagaccg tgtcgatgaa cgtcacgctg gtatgctcga
      481 tcccatgcat gtccagcaga tggaccatgt actgatgcgg gcggtccacc gagatgaaca
      541 ggccggggtt gccctcgccg atgagggcct tgaggaggac cagctccacc accacgaact
      601 gggtggtcct gacgtccagt accatgagcc ctggtcttgg gactggcccg agctgcctgc
      661 gaaagttctc gtattggcgc atgtccctgg cgttcatcgg cgtccgttcc tgggtagcat
      721 ggtccggggt atttagctac tttctggtgc ttattggaat tgatatcgac cctgctggtg
      781 gctggagccc attctctatt aaatatccag agcgagatgg aggagggatc gctttgctag
      841 gtggcgacgc cctcgggctg atcatggtct atgcctatgt aggtgccata gcggcgctcg
      901 ccgccaagtg gcgcttcctc agggaacggt ccctccagag gaagttcatc cacgtgatgg
      961 tcggcaacat cgccctgttc tggtgggtct tcgacaccag gtatgccatg gccctgttgg
     1021 cggccgcccc tttcatcccc gtgctcctcc tgttctctag aaaggcgggg gaaggggatg
     1081 gga
//
LOCUS       DAOSYN010000385         1078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_98722_length_1078_cov_2.128055, whole genome shotgun sequence.
ACCESSION   DAOSYN010000385 DAOSYN010000000
VERSION     DAOSYN010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98722_length_1078_cov_2.128055"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLJ11_09005"
     CDS             complement(<1..998)
                     /locus_tag="PLJ11_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO04845.1"
                     /translation="MPLTNEQVLNPIVFAEKVNRQYLRYRLSRLPLKDVRLKEQSERL
                     VGSPDRSPFIKGPYVSLSRPILPGGTVEDLCRDGTFHSALHGQGIAEFAQLYKHQERA
                     ARAIRGGRNLIISTGTGSGKTEAFLYPIISKCLELKEQGRLRGVAAVLLYPMNALAND
                     QNERLRRLLAGTGITFGTYTGATIEERSKADQAFRRLAPGQGREVYLNALRNPGGDRR
                     TVIPWEERASVEEMRESPPNLIISNVKQLELILTRAEDVGIFVDNSLSFIVVDEAHTF
                     TGVGGSEVAILVRRLREFTGRRPEDVVHVGTSATIVDPEGDSNRAAIRFASRFFGVR"
BASE COUNT          176 a          337 c          327 g          238 t
ORIGIN      
        1 cgaacaccga agaaccgcga ggcgaacctg atggcagctc tattgctgtc cccctcgggg
       61 tccacgatcg tcgcgctcgt gcctacatgg acgacatcct ccggtcttct gcctgtgaac
      121 tcccttaggc ggcggaccag gatggcgacc tcggagccgc cgacccctgt gaatgtgtgg
      181 gcctcgtcca cgacgatgaa ggatagcgaa ttgtccacga agatccccac gtcctcggcc
      241 ctggtgagaa tgagctcgag ctgcttgaca ttgctgatga tcaggttggg aggggactcg
      301 cgcatctcct caacgctcgc cctctcctcc catgggatga ccgttcgcct gtccccgcca
      361 gggttcctga gggcgttcag gtagacctcc ctgccctgcc ctggggcgag ccttctgaag
      421 gcctggtccg ccttgctcct ctcctcgatc gtggcaccgg tataggtacc gaaggtgatg
      481 ccggtcccgg caagcaggcg gcgcaggcgc tcgttctggt cgttggccaa ggcattcatg
      541 gggtagagga ggacggcggc gacccctcgc aatcgccctt gctccttgag ctccaggcat
      601 ttgctgatga tgggatagag gaacgcctcc gtcttcccgc tgcctgtacc ggtggagatg
      661 atgaggttcc tgccccccct gatggctctc gccgccctct cctgatgctt gtagagctgg
      721 gcgaactcag cgatcccctg cccatgcagg gcgctgtgga aggtgccgtc cctgcacagg
      781 tcctccacgg tcccgcccgg aaggatcggg cgggagagcg agacgtaggg gcctttgatg
      841 aagggcgagc ggtccgggct gcccaccagc ctctctgact gctccttcag gcggacgtcc
      901 ttgaggggga gcctggaaag acggtagcga aggtactgtc gattcacttt ttcggcgaac
      961 actatgggat ttaggacctg ttcattggtc aagggcatca cttcatgtaa ctttcaagcg
     1021 tgggcacgga tatctttccc tcggcccttc caatgttatt gttctttcct tcctttct
//
LOCUS       DAOSYN010000386         1072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_99463_length_1072_cov_3.548673, whole genome shotgun sequence.
ACCESSION   DAOSYN010000386 DAOSYN010000000
VERSION     DAOSYN010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99463_length_1072_cov_3.548673"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLJ11_09010"
     CDS             complement(<1..476)
                     /locus_tag="PLJ11_09010"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOO04846.1"
                     /translation="MNTIGSELRLTLFGASHGPCVGAVLDGVPPGLTVDLARLQGEVD
                     LRRPRAGIGTPRAEEDRVEVLSGLLDGRSTGAPITLLVMNRDIDSQRYERFRTVPRPG
                     HADLTARSKYSECADLRGGGQFSGRMTVGIVAAGAIAKMLLEERGVRIAAYVRQVGG"
     gene            complement(473..>1072)
                     /locus_tag="PLJ11_09015"
     CDS             complement(473..>1072)
                     /locus_tag="PLJ11_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOO04847.1"
                     /translation="RPQTYTVPGDCSSAAFPLAAGALTGRVTVRNLDPADAQGDRAFL
                     DILTGLGAEVEWRGDALTCSRGELRGAVIDLGDAPDLFPITAVMCAYARGESRIYNAA
                     HVRLKESDRIAATVQFLRAMGAEVRETADGCVVEGGRPLKGAHVDARNDHRILMAAAV
                     AAMAAEGETVIGDGECHRISYPDFVKDMRSLGARMEMIP"
BASE COUNT          138 a          416 c          351 g          167 t
ORIGIN      
        1 cccccgacct ggcggacgta ggcggcgatg cgcacccccc gctcctccag gagcatcttg
       61 gcgatggcgc cggcggccac gatcccgacg gtcattcggc cggagaactg ccctccgccg
      121 cgcaggtcgg cgcactcgga gtacttcgag cgcgcggtga ggtcggcgtg ccccggccta
      181 gggaccgtcc ggaaccgttc gtatctctgc gagtcgatgt cccggttcat cacaagcagg
      241 gtgatcggcg cccccgtgct ccttccgtcc agaaggccgg agaggacctc cactcggtcc
      301 tcctccgccc tcggggtccc gatgcccgcc ctcggccgtc tgaggtccac ctcgccctgc
      361 aggcgggcga ggtccaccgt caggccgggg gggaccccgt ccagcaccgc gccgacgcag
      421 ggcccgtggc tggccccgaa cagggtgagg cgcagctcgc ttcctatcgt gttcatggta
      481 tcatctccat cctggccccc agggaccgca tgtccttgac gaaatcgggg tatgagatgc
      541 gatggcattc gccatcgcct atgaccgtct ccccctcggc cgccatggcc gccacggccg
      601 cggccatcag gatgcggtgg tcgttccttg cgtccacatg cgcccccttc agcggccttc
      661 ccccctccac cacgcacccg tccgccgtct cccgcacctc cgcccccatg gccctcagga
      721 actggaccgt ggcggcgatg cggtcgctct ccttgaggcg gacgtgcgcc gcgttgtaga
      781 tgcggctctc cccccgggcg tacgcgcaca tcaccgcggt gattgggaat aggtccggag
      841 cgtcacccag gtcgatgacc gcgccgcgga gctccccgcg ggagcaggtg agggcgtccc
      901 ccctccattc cacctccgcc cccagccccg tcaggatgtc gaggaacgcg cggtccccct
      961 gggcgtccgc tgggtcgagg ttgcgcacgg tcacccggcc ggtgagggcc cccgcggcca
     1021 gcgggaaggc ggcggaggag cagtcccccg gcaccgtgta ggtctgcggc cg
//
LOCUS       DAOSYN010000387         1066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_100372_length_1066_cov_1.912957, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000387 DAOSYN010000000
VERSION     DAOSYN010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100372_length_1066_cov_1.912957"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..909
                     /locus_tag="PLJ11_09020"
     CDS             139..909
                     /locus_tag="PLJ11_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO04848.1"
                     /translation="MDGRGSLGLLAMGYDRDEYRTKQPQDFISMFYPELSKELKDCTV
                     LDVGCGRGAVSLSIAPYAKSVLGIDIDEGNIELCRERAEAMGLTNVRFVKLSAFDLRP
                     VERYDVVVLSDVLEHVQEQKALLERCIDMMAPGGVMYLNTPNRWFPLEPHKRLPFLSY
                     LPKGMADHYAKAFGKGSYDGYHLLSYGQFCSLLDSLPIRYSFKVQPNPRRRLYRIAAP
                     LVTNAPCLWRFANAFQVIVQRNASLIDANILDNKIINP"
BASE COUNT          229 a          306 c          303 g          228 t
ORIGIN      
        1 cgatcattag gggggatagg aaatagaagg ggcacggccg cgcaacgagg gcgagggcgg
       61 cctccatcgc ccgcccttct ctggggatat aatcccttcc ctcaggccta tagaacgata
      121 tacatcccag gtccatccgt ggacgggaga ggttccttgg gcctgttggc aatgggctat
      181 gatcgtgacg agtaccgcac caagcagccg caggacttca tttcgatgtt ctatcccgag
      241 ctgagtaagg agctcaagga ctgcaccgtg ctcgacgtcg ggtgcgggag gggtgcggtc
      301 tctctcagca tcgctcccta cgccaagagc gtcctgggga tcgacataga cgagggaaac
      361 atcgagcttt gccgggaaag ggctgaggcg atggggctga ccaacgtccg cttcgttaag
      421 ctctccgcct tcgacctgcg gcccgtggaa cggtacgacg tggtggtcct gagcgacgtg
      481 ctcgagcacg ttcaggaaca aaaggcgctg ctggagcggt gcatcgatat gatggccccg
      541 ggaggggtga tgtacctgaa cacgccgaac agatggttcc cactggagcc gcacaagcgg
      601 cttccgttcc tgtcctatct ccctaagggg atggcggacc attatgcaaa agcgttcggg
      661 aagggctcct atgacggcta tcacctcctc agctacggcc agttctgcag tcttctcgac
      721 tccctcccca tacggtactc gttcaaggtt cagcctaacc cgcgacgtag actgtaccgc
      781 atcgccgccc ccttggtcac caacgccccc tgcctctggc gcttcgccaa cgccttccag
      841 gtgatcgttc agaggaatgc atctctaata gatgctaaca ttttggataa taaaataatc
      901 aatccttaat ccaataatga gttatttctt atccaccgac ttcgtgaaag ctaatcgatc
      961 tcatgcaagg tcctcccgga ttataaagtc tgccagatag acctccatcg gagttctgag
     1021 gtcgagggcc cagtggggtc tctcggcgtt gtagaacgca aggctg
//
LOCUS       DAOSYN010000388         1065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_100452_length_1065_cov_3.586139, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000388 DAOSYN010000000
VERSION     DAOSYN010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100452_length_1065_cov_3.586139"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLJ11_09025"
     CDS             <1..367
                     /locus_tag="PLJ11_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04849.1"
                     /translation="EVETGYVPPAHALDPMDYIRARIASKIARYSNHCHKFSLAAPPH
                     YVMPVMPFFIRPPRDRGMDEVAVVKHYCDLYYKSPPVSVEEIMNSRIHSVSIIDVEDV
                     TLRDMAPDEYINLTVDWYL"
     gene            377..997
                     /locus_tag="PLJ11_09030"
     CDS             377..997
                     /locus_tag="PLJ11_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOO04850.1"
                     /translation="MDSALLFLGSVAVISLSGVLMPGPVFAVTIACGFQDRWSGWKIA
                     LGHALVEVPTIAAIFLGLSAFLKDEALLTAIGLLGGAVLLYLGFDVVRTRKEVVMDCN
                     GKYKSPFWAGVMTTAANPGWLLWWATVGAGLTATAVTFGLWMLPLFVIVHVTCDFLWE
                     GFVGMTVFGTKGRWSTAWHQRVIAASGALMVVFALLFIYDALGNLL"
BASE COUNT          172 a          336 c          339 g          218 t
ORIGIN      
        1 ggaggtggag accgggtacg tgcccccggc gcacgccctg gatccgatgg actacatccg
       61 ggcgcggata gcgtccaaga tcgcaaggta ttcaaatcac tgccacaagt tctcgttggc
      121 cgcccctccg cactacgtca tgccggtgat gcccttcttc atccgccctc cgagggaccg
      181 ggggatggac gaggtggcgg tggtgaagca ctactgcgac ctgtactaca agagcccgcc
      241 ggtgagcgtg gaggagatca tgaactcgcg catccacagc gtctcgatca tcgacgtgga
      301 ggacgtcacc ctccgggaca tggccccgga cgagtacatc aacctcacag tggactggta
      361 cctctgaggg gatgccgtgg actccgcgct cctcttcctc ggctccgtcg ccgtcatctc
      421 cctttccggc gtcctgatgc ccggtcccgt cttcgcggtc acgatcgcct gcggcttcca
      481 ggaccggtgg tccggatgga agatagccct ggggcacgcc ctcgtcgagg tgccgaccat
      541 cgccgccata ttcctcggcc tgagcgcctt cctcaaggac gaggcgctgc tgacggccat
      601 aggccttttg ggcggcgccg tgctcctcta cctggggttc gatgtcgtgc gcacgcggaa
      661 ggaggtcgtc atggattgca acgggaaata caagagcccc ttctgggcgg gggtgatgac
      721 caccgccgcc aaccctggct ggctcctgtg gtgggcgacc gtgggggcgg gcctgacggc
      781 cacggcggtc acgttcggcc tgtggatgct gcccctcttc gtcatagtgc acgtgacgtg
      841 tgacttcctg tgggaggggt tcgtgggcat gacggtcttc ggcaccaagg gaaggtggag
      901 caccgcctgg catcaacggg tcatcgctgc ctccggggcg ctcatggtcg tcttcgccct
      961 gctgttcatc tatgacgcct tggggaacct gctgtgacct catcttttta aacgtcccct
     1021 gtcttactga catgcggaga tgtccaggac ttataaggac tataa
//
LOCUS       DAOSYN010000389         1060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_101183_length_1060_cov_2.149254, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000389 DAOSYN010000000
VERSION     DAOSYN010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101183_length_1060_cov_2.149254"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLJ11_09035"
     CDS             <1..428
                     /locus_tag="PLJ11_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO04851.1"
                     /translation="IPLLYALAMGADAISAMLLGKIFDRGGPRVLLAVIIPAAFFPVL
                     AFSDSMVLIAAGMGLFGLGMGAQESIMRAVVADLAPVGKRATAYGYYNTIFGSFWFIG
                     STALGVLYDVSIPTMITVSVGLQLLFIPVFLMFLRDRGG"
     gene            complement(598..>1060)
                     /locus_tag="PLJ11_09040"
     CDS             complement(598..>1060)
                     /locus_tag="PLJ11_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOO04852.1"
                     /translation="NEAQMFKAMALGDLGEGPLVKAIAMARAPLLTVMKSANFLRLAE
                     TNALPGSFTAAYGSDPRTFFVAAQEIERRYPGLRLGKDIPYGAVGEYTYLVDRLGEGL
                     KQLMAGSRKFSLDRIRRTDIASLTPYAEKVTGIETLENMAERVMLPMLADW"
BASE COUNT          170 a          336 c          343 g          211 t
ORIGIN      
        1 tcatccccct cctatacgcc ttggccatgg gggcggacgc catctcagcc atgctgctgg
       61 ggaagatctt cgaccgcggg ggccccaggg tgcttctggc ggtgatcatc cccgcagcct
      121 tcttcccggt gctggccttc tccgattcca tggtgctgat cgcggcaggg atggggctct
      181 tcggactggg gatgggggcc caggaatcga taatgagggc ggtggtggcg gacctcgccc
      241 ctgtggggaa gagggcgacg gcctacggct attacaacac gatcttcggt tcgttctggt
      301 tcatcggctc gacggcattg ggggtactgt atgacgtgtc catcccaacg atgataacgg
      361 tctccgtggg cctgcagctg ctcttcatcc cagtgttcct gatgttcctg agggaccggg
      421 gcggatgacc ggaggccgga aggaggcttg accgcccgat atgaccggca gttctatccg
      481 cgtcctcgct cagcatgatg gcgggagcgg cagcaatgcc tcccgagacc tattcggggg
      541 tgggcgcatc cgtccgggac catgtctaaa aaaaggaaaa gggttttcgc agggccgtca
      601 ccaatccgcc agcatcggca gcatcaccct ttcggccatg ttctccaggg tctcgatccc
      661 ggtgaccttc tcggcgtagg gcgtgaggga ggcgatgtcg gtgcgcctga tccggtccaa
      721 ggagaacttc ctgctccccg ccatcagctg cttcaatccc tcccccaggc ggtccacgag
      781 gtaggtgtac tctcccacgg ccccataggg gatgtccttc ccaagcctca gccccggata
      841 gcggcgctcg atctcctggg cggcgacgaa gaaggtacgg gggtcgctgc cgtaggcggc
      901 cgtgaagctg cccggcaggg cgttggtctc cgccaacctc aggaagttgg cgctcttcat
      961 taccgtaagg aggggcgccc gggccatggc gatcgccttc accagcggcc cctccccgag
     1021 gtcccccaag gccatcgcct tgaacatctg ggcctcgttg
//
LOCUS       DAOSYN010000390         1056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_101716_length_1056_cov_2.167832, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000390 DAOSYN010000000
VERSION     DAOSYN010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101716_length_1056_cov_2.167832"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1056)
                     /locus_tag="PLJ11_09045"
     CDS             complement(<1..>1056)
                     /locus_tag="PLJ11_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOO04853.1"
                     /translation="LLQDLKKAGRVGSTDPRRIDRGDIQALQGLYKSKRLDPSTQKKY
                     NQLLNGLLKFFGNFIIEQMKAEGVRFPKESRKPIRALSEESTDMLLNALKDYDDWEGS
                     IGLGIVALGLSTGCRPKEIRMAHLEDLDLKRGRFYVRYPKGEGSWASPQWISIVREDL
                     IPFLESYLQAREEHLAHKGVKEHHALFPAVGYGNKTGFYSASNLNRIKRNLEERTGVE
                     FRIKDLRPTMASQTVDMDPTLLNDVSSQLRHESPKTTSRYYAEIKVDKAGERLKRAWA
                     SNRLLSTRIISLILSQHSTHLQNNPMMHSGRSLRCMAIAGVSAISQHRPPCMRPLYQS
                     MNSARLANRQRWRSYSS"
BASE COUNT          186 a          311 c          311 g          248 t
ORIGIN      
        1 tggctggagt acgaccgcca tcgctggcgg ttcgccagcc tggcggagtt catcgattgg
       61 tacaacggcc gcatgcacgg cggcctgtgc tgggagatcg ccgagacgcc ggcgatggcc
      121 atgcagcgca agctgcggcc agagtgcatc attggattgt tctggaggtg cgtggaatgc
      181 tgagaaagaa tgagcgaaat aattcttgtg ctcaacagtc gattgctggc ccatgcccgc
      241 ttgagccttt cccccgcctt gtccaccttg atctcggcgt agtaccggga ggtggtcttg
      301 gggctttcat gccgcaattg ggagctcacg tcgttaagaa gcgtcgggtc catgtccact
      361 gtttggctgg ccatggtcgg ccttaggtcc tttatacgaa actccacgcc ggtcctctcc
      421 tccaaattcc gttttatgcg gttgaggttc gaggcggagt agaaccccgt tttatttcca
      481 taaccaacgg ccggaaacag ggcatggtgc tccttcacgc ccttatgggc taagtgctcc
      541 tccctcgcct gcagatagct ctccaagaag ggtataaggt cctccctgac gatcgatatc
      601 cactgcgggg aggcccagct gccctcgccc ttgggatagc gcacgtagaa ccttcccctc
      661 ttgaggtcaa ggtcctccag gtgcgccatc ctgatctcct tgggtctgca cccggtcgac
      721 aagccgaggg cgacgatacc gagccctatg gacccttccc aatcgtcata gtccttcagc
      781 gcgttgagca gcatgtccgt gctttcctcg ctcaaagccc ggatgggctt gcgggactcc
      841 ttgggaaaac gcacgccctc ggccttcatc tgttcgatga tgaagttgcc gaagaacttc
      901 agaaggccgt tcagaagctg attgtatttc ttctgcgtgc tggggtccag gcgcttcgat
      961 ttgtacaggc cctggagggc ctgtatatcg cccctgtcga tccgtcgggg gtcggtggaa
     1021 ccgaccctgc ccgccttctt gaggtcctgg aggagg
//
LOCUS       DAOSYN010000391         1053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_102116_length_1053_cov_2.263527, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000391 DAOSYN010000000
VERSION     DAOSYN010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102116_length_1053_cov_2.263527"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1053)
                     /locus_tag="PLJ11_09050"
     CDS             complement(<1..>1053)
                     /locus_tag="PLJ11_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04854.1"
                     /translation="SGSISLAIGGISVRFTAQGGSAVNGTAVTTADGSFTASLYPGMY
                     TVSVDQDIDPGDPSERYQSLSAMDLEVKVGSDPEPLAIEVVTRVLVNGAVSPNGSATI
                     VFDGPERTVISARATYSLYLLRGSYSVYVQVNTSALRSASLQRMDVSGPTTEDLVAES
                     AAQVVLRAQLSGVSAQAVELSIEHAGAYYNTTTATSGTATVYLPNGAYTVSVDHATTA
                     TLRGVLRHVRYIGQANFSVSGTFKNVVISTTYALNNATVHGQVLSSGAPTAASLRFEA
                     QSATAISLSVDALGSYSVQLAVGSYSVYAVSTDSSHAYLGTLEVGAPGDIPFNISLRE
                     AFRLSGVTFANDEGVRA"
BASE COUNT          171 a          324 c          376 g          182 t
ORIGIN      
        1 gcccgcaccc cctcatcgtt ggcgaaggtc accccgctca gccggaaggc ctcccgcagc
       61 gatatgttga acggaatgtc ccccggcgcg ccgacctcca acgtccccag gtaggcatgg
      121 gagctgtccg tgctgacggc gtagacgcta taggagccga cggcgagctg caccgaatag
      181 cttccaagag catcaacgga aagggagatg gccgttgcgc tctgcgcctc gaaccgcagg
      241 cttgccgccg taggggcgcc agaggagagc acctgcccgt gcacggtggc gttgttgagg
      301 gcgtatgtag tgcttataac cacgttcttg aacgtcccgc tgacggagaa gttggcctgg
      361 ccgatatagc gcacgtgcct gaggacgcct ctaagcgtgg ccgtggtggc atggtccacg
      421 gagacggtgt aggcgccgtt gggcaggtag accgtcgcgg tcccgctagt ggccgtggtg
      481 gtgttgtaat aggccccggc atgctcgatg ctcagttcga ccgcctgggc gctgaccccc
      541 gagagctggg ctcgcaggac cacctgggcg gcgctctccg cgaccaggtc ctcggtggtc
      601 ggaccgctca cgtccatgcg ttgaagggag gcggagcgca gggcggaggt gttgacctgg
      661 acatatacgg aatagctccc cctgagcagg tagagggagt atgttgccct ggccgagatg
      721 accgtccgtt cggggccgtc gaagacgatg gtggcgctcc cgttcgggct cacggcgccg
      781 ttgaccagca ccctggtgac gacctcgatc gcgagcggtt cggggtcgct gccgaccttg
      841 acctccaggt ccatggccga cagggactgg tagcgctccg acgggtcgcc ggggtcgatg
      901 tcctggtcca cgctgacggt gtacatgccg gggtacaggg acgcggtgaa gctcccgtcc
      961 gcggtggtga ccgcggtccc attgaccgcg gagccgccct gggcggtgaa tcggacggaa
     1021 atgccgccta tggccaggga gatgctcccg ctg
//
LOCUS       DAOSYN010000392         1052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_102217_length_1052_cov_4.720160, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000392 DAOSYN010000000
VERSION     DAOSYN010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102217_length_1052_cov_4.720160"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..950
                     /gene="dcd"
                     /locus_tag="PLJ11_09055"
     CDS             462..950
                     /gene="dcd"
                     /locus_tag="PLJ11_09055"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOO04855.1"
                     /translation="MCLVPDHEIEARLRDGSLVITDFMLECLTPNGYDLRVAEARLEG
                     EESVTLGTLVIPPGRLFFVSTVERVELPSDLAAQLWARTSWLRKGLLMGLGKVDAGFH
                     GTLTFSGLNASSAPVDLPIGSRMVQMVLEHMHSPVERTYERRSGNYQDQAGITLEPSG
                     EQ"
BASE COUNT          176 a          338 c          364 g          174 t
ORIGIN      
        1 gtgaagtaga gcatgtcgcc caccttgacc ttgccatagg gggagcggcg cccgttgaac
       61 gcccggccga ccattgtctt cctgccctgg cgcatcgcct ccagggcgcc ggcggccttg
      121 tccaagtaaa cgatgtggtc cataccgtgc cctcatcggc ttggaaggat aaatgttgat
      181 tgccccgggg tccgcctgga agggaaggag caccggagga aatgcccctt aggagagggg
      241 gcgatgccca ggcgcctcca ggatgcctcc gcgcagctca ggaggggcgt ctgccccgcg
      301 gttcatggga acgcatggcc ataggagggc gcgacgggcg tgacctgccg ggggcggtgg
      361 ggccgtcccc ttccccgggg atgcggcccc tccaccgccc tcgttccgtc agggagggcg
      421 taagcgcaac ctctaaccgt tccgacggcc tacgggcatc catgtgcctg gtccccgacc
      481 atgagataga ggctcgcctc agggatggtt ccctggtgat caccgacttc atgctggagt
      541 gcctgacccc caacggctac gacctgcggg tggccgaggc gaggttggag ggggaggaaa
      601 gcgtcacctt gggcacgctc gtcatcccgc ctgggaggct cttcttcgtc tccaccgtgg
      661 agagggtgga gctcccttca gacctggcgg cgcagctatg ggcccggaca tcatggttga
      721 ggaaggggct gctcatgggg ctgggcaagg tggacgcggg cttccacggg acactgacct
      781 tcagcgggct caacgcctcc tccgccccgg tcgacctgcc catcggaagc cggatggtgc
      841 agatggtgct tgagcacatg cactcccccg tggagcgcac gtacgagcgg cgcagcggga
      901 actaccagga ccaggcgggc atcacgctag agccatccgg agagcagtag gtcggcggag
      961 aagaccgagg ccgtgggcga gtaggtcctg agttcctcgc ccctgagcac ctccagctgc
     1021 acgcccatgc cgttcgcctt cagcacgagg tc
//
LOCUS       DAOSYN010000393         1047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_102954_length_1047_cov_2.941532, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000393 DAOSYN010000000
VERSION     DAOSYN010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102954_length_1047_cov_2.941532"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLJ11_09060"
     CDS             <1..542
                     /locus_tag="PLJ11_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO04856.1"
                     /translation="DLDKLAQISSLKKQYFPVYMFKRDVNGMEHVYIEPAASTTLPGL
                     HNLKVPGGDLKIFDDRFDTGGAELVKVDIEMGPYLSSLPGTAKEQALVYFPIWRLDYA
                     FEQRAYVAVISASSGEVFSGDFPKRSSGAYMAVAGLGFLAFLAEGLLAIASPVLAGAL
                     MAMTVAGVFGASNYVARRM"
     gene            545..>1047
                     /locus_tag="PLJ11_09065"
     CDS             545..>1047
                     /locus_tag="PLJ11_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04857.1"
                     /translation="MTISVGLSCPACGGAIQVAEGDTVLNCGYCGSLLWAEGDAGVMT
                     VAYRNVQGRDSVLRATEQWWRKGWKARDLRAKGRLLECYPIYLPFWSTTTRVAGWICG
                     YEERRHTDREGNVRTERVPMEEMVLNDYRYTSIACDPGDLGIRYLRNVGGEASFADFE
                     SIPTFEST"
BASE COUNT          194 a          310 c          362 g          181 t
ORIGIN      
        1 aggacctgga caagctggcc cagatctctt ccctgaagaa gcagtacttc cccgtctaca
       61 tgttcaagag ggacgtcaac ggtatggagc acgtctacat tgaaccggcc gcctcgacga
      121 ccctccctgg cctgcacaac ctcaaggtgc cggggggcga cctgaagata ttcgacgacc
      181 gcttcgacac cggcggcgcc gagctcgtca aggtcgacat cgagatgggg ccctatctaa
      241 gcagccttcc tgggacggca aaggagcagg ccctcgtcta tttcccaata tggaggcttg
      301 actacgcctt cgagcagagg gcgtacgtcg cggtgatcag cgcctcctcc ggggaggtct
      361 tctccggcga tttcccgaag cggagctccg gagcgtacat ggccgtggcc ggtctgggct
      421 tcctggcctt cttggccgaa gggcttctgg caatagcgag cccggtcctg gcgggggcgc
      481 tgatggccat gaccgtcgcc ggcgtcttcg gggcgtccaa ctacgttgcg cggaggatgt
      541 aggcatgacg atcagcgtgg ggctgagctg cccggcatgc ggcggtgcca tccaggtcgc
      601 cgaaggggat accgttctca attgcgggta ctgcggctcg ctgctttggg ccgaggggga
      661 cgccggggtg atgaccgtgg cctaccgcaa cgtgcagggc cgcgattccg tgctcagggc
      721 gacggagcag tggtggagga agggatggaa ggccagggac cttagggcca agggaaggct
      781 gctggaatgt taccccatct acctcccgtt ctggagcacc accacccgcg tggccggctg
      841 gatatgcggc tatgaggagc ggaggcatac ggaccgcgaa ggcaacgtcc ggacggagag
      901 ggtcccgatg gaggagatgg tgctgaacga ctaccgctac acgagcatcg cctgcgaccc
      961 gggcgacctg gggataagat acctcaggaa cgtggggggc gaggcctcct tcgccgattt
     1021 cgagagcatc ccgaccttcg agagcac
//
LOCUS       DAOSYN010000394         1044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_103387_length_1044_cov_3.194135, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000394 DAOSYN010000000
VERSION     DAOSYN010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103387_length_1044_cov_3.194135"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1044
                     /locus_tag="PLJ11_09070"
     CDS             <1..>1044
                     /locus_tag="PLJ11_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04858.1"
                     /translation="QKVPRIYANNLLFLVADEEQKALMEAKAIEYLALSQLVKDIEEG
                     HSYLASMSSQQKDRLKKAKQEAELYIKVAIVVCYRHVVLPSVQSNLTSQGGRPMRILS
                     MRVSDTEAKNNLANNMGQDQVLVDFLRDNKVAMTSDDSISPEYILSQLWEKRKESMTT
                     DDFRKMFFKNPVCGLILTEDIIRKSLKEGVKDGDWVVIVGKEIYDKKNHSSFTAPFDS
                     TSTIILTGTETYKEQTAEFYCAKCGARRPCKEHEGEGEISLPGPSMEPKCPKCGRKVK
                     ECICPSQRTLRFDRLKIDRIQEDLKLKIIDDDIKKFEWIRIKPTNRNSLIKLTNALPQ
                     FGKVDMELHVYLVI"
BASE COUNT          295 a          267 c          280 g          202 t
ORIGIN      
        1 cagaaggtcc cacgcatcta tgccaataat ctcctgttcc tggtggccga cgaggagcaa
       61 aaggcattga tggaggccaa ggccatcgaa taccttgccc tcagccagct ggtgaaggac
      121 atcgaagaag ggcattccta cctcgcctcc atgtcctccc agcagaagga ccgcctgaag
      181 aaggcgaagc aggaggccga gctctacatc aaggtggcca tcgtggtctg ctatcgccac
      241 gtcgtgctgc cgtccgtgca atcaaaccta acatctcagg gagggagacc gatgcgcatc
      301 ctttcgatgc gcgtatcgga cactgaagcg aagaacaacc tcgccaacaa catgggccag
      361 gaccaggtcc tggtggactt cctgcgggac aacaaggtgg ccatgaccag tgatgacagc
      421 atctctccag agtacatact gtcccaactc tgggaaaagc gtaaggaatc aatgaccacc
      481 gatgacttcc gcaagatgtt cttcaagaac ccagtctgcg gtctgatatt gaccgaagat
      541 attattcgta agtcgttgaa ggaaggggtg aaggatggtg actgggtggt catcgttgga
      601 aaggagatct acgataaaaa gaaccacagt tcgttcaccg cccccttcga ttccacttcg
      661 accatcatct tgacggggac cgagacgtac aaggaacaga ccgccgagtt ctactgcgct
      721 aaatgcggtg ctagaagacc atgcaaggag catgagggcg aaggtgagat atccctccca
      781 ggtccgtcca tggaaccgaa atgcccgaaa tgcggaagga aggtcaaaga gtgcatatgc
      841 ccctcacaaa ggacgctgag gttcgatcgt ttaaagatcg ataggataca agaggatctg
      901 aagctgaaaa taatcgatga cgatataaaa aagttcgaat ggatacggat caagccgacg
      961 aaccggaact cattgatcaa attgaccaac gccttgccgc agttcggcaa ggtggacatg
     1021 gaactccatg tgtacctcgt tatc
//
LOCUS       DAOSYN010000395         1040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_104066_length_1040_cov_1.563452, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000395 DAOSYN010000000
VERSION     DAOSYN010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104066_length_1040_cov_1.563452"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PLJ11_09075"
     CDS             complement(<1..901)
                     /locus_tag="PLJ11_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04859.1"
                     /translation="MTAEPMVEPKPRKDRTINTPGRVFPDGSIIEQCWDGNKAYYYYM
                     DVNGNIKAEDQHVGENGFTYAPINEEILEKRIVLLPTYPAPYDSEAELLRDIQLYIHQ
                     WCEVDMGTERLLALYVMLTWIHDHDDCQTMPIINARGRSGTGKSRLLETLRQICYRGM
                     RASGCLSFSSMFRTAERWKGTLAINEGDLKNSSEKSDMVKYLNERYEKGGNVWRTNPE
                     TLKSEYFNAYGPTIVTTRHQFKDDALESRCFTIPMKERTRRDIYLNLPKEFYDAGATL
                     RNHLLCFRFKRLGSFVNDYTLEFD"
BASE COUNT          229 a          274 c          250 g          287 t
ORIGIN      
        1 cgtcaaattc tagggtatag tcatttacga agctgcctag ccgcttgaat ctgaagcaca
       61 gaaggtggtt gcgcaaggtg gctccagcat catagaattc cttcggcaga ttcaggtaaa
      121 tgtcccgcct tgtacgctcc ttcataggga tggtgaagca cctggactct agcgcatcgt
      181 ccttgaattg atgacgggtg gtgacgatgg tgggcccgta agcattaaaa tattcgctct
      241 tgagggtttc agggttggtc ctccatacat tacccccctt ttcataccgc tcgttgagat
      301 atttcaccat atccgatttt tcactcgagt tcttcaagtc gccctcatta atggctagag
      361 ttcccttcca tcgctcggcg gtcctgaaca tagaagagaa gcttaggcag ccggatgctc
      421 tcatcccacg gtagcatatc tgcctaagcg tctccaatag ccgggacttg cccgttccag
      481 agcgtcccct ggcattgatt atgggcatgg tttggcaatc gtcatggtca tggatccagg
      541 tgagcatgac atataaagcc aggagccttt cggtgcccat atctacctcg caccactgat
      601 ggatatagag ttgaatgtcc ctcagcagtt cggcctcact atcgtagggg gccggatatg
      661 ttggtaatag gactatccgc ttctccagga tctcttcatt aatgggagca tatgtgaacc
      721 cgttctcgcc aacgtgctgg tcctcggcct taatgttacc attcacatcc atatagtagt
      781 agtaagcctt gttaccgtcc cagcattgtt cgataatact gccgtcaggg aacactctgc
      841 ccggtgtatt aatggtccta tcctttctgg gctttggctc gaccatcggc tcggccgtca
      901 ttcgaccacc tcctttttct cgtcctttgc accttttcga aggggtcgta ctaggagctc
      961 gccatcgctg aaaacttcca cccaaggatg cttatctaga tcatgctcgt gcacgaaagg
     1021 gattggaaga acaagaagat
//
LOCUS       DAOSYN010000396         1038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_104271_length_1038_cov_3.490336, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000396 DAOSYN010000000
VERSION     DAOSYN010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104271_length_1038_cov_3.490336"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLJ11_09080"
     CDS             complement(<1..980)
                     /locus_tag="PLJ11_09080"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOO04860.1"
                     /translation="MEGTPLSNGYYMPAEWTRHDATWLSWPKNTLTFPEEVLGAVEGT
                     FAQMAAALSEGEAVRVLVDDAQAEERAEAIIARAGANMARIEFVRIRSADVWIRDYGP
                     TFLLHRETGRKAAVKWTFNAWGGKYDDLLYDDVTGREIVERSGVPCFQPGIVLEGGSI
                     DVNGEGLVLTTEQCLLNRNRNPHLDRSQIEGFLRHYLSVEDVLWLSSGIAGDDTDGHI
                     DDFARFVSRSTVLCARAEKGEDAPVLARNWELLGRYKDRFGLEVQALPMPRPLFLEEE
                     EQNLPASYANFYIGNRVVLAPVFGAPADRAALETLSSHFPGREVVPIPAR"
BASE COUNT          163 a          372 c          322 g          181 t
ORIGIN      
        1 tcccgggccg gaatgggcac gacctcccgt cccgggaaat gggaggagag ggtctccagg
       61 gccgcgcggt cggcgggggc cccgaacacc ggggcaagga cgaccctgtt gccgatgtag
      121 aagttggcat agctcgctgg taggttctgc tcctcctcct ccagaaagag gggccggggc
      181 attggaaggg cctggacctc caggccgaag cggtccttgt accgcccgag gagctcccag
      241 ttcctcgcca gcaccggggc gtcctccccc ttctcggccc gggcgcagag caccgtggag
      301 cgggagacga agcgggcgaa atcgtctatg tgcccgtcag tatcatcccc cgcgatcccg
      361 gaggagagcc acaggacgtc ctctacggaa aggtagtggc gtaggaaccc ctcgatctgc
      421 gaccggtcca gatgggggtt ccggttcctg ttcagcaggc actgctcggt cgtcagcaca
      481 agcccctcgc cgttcacatc tatggagccg ccctccagca ctatcccggg ctggaagcag
      541 gggacgcccg accgttccac tatctcccgc cccgtcacgt cgtcgtagag caggtcgtcg
      601 tacttgcccc cccaggcgtt gaaggtccac ttcaccgcgg ccttccttcc ggtctccctg
      661 tgcagcagga acgtcggccc gtagtccctg atccaaacgt ccgcgctcct gatgcggacg
      721 aactctatgc gggccatgtt cgcccccgcc cgggcgatga tggcctcggc cctctcctcc
      781 gcctgtgcgt cgtccacgag gaccctcacg gcctccccct cgctcagggc ggcggccatc
      841 tgggcgaagg tgccctccac cgcccccagg acctcctccg ggaaggtcag ggtgttcttc
      901 ggccaggaga gccaggtcgc atcgtgcctc gtccactcgg cgggcatgta atatccgttc
      961 gacagcggtg ttccctccat gtcctccccc tcatggcgaa aggcctgtgc ggacgatcgc
     1021 cccataacat tcagggcg
//
LOCUS       DAOSYN010000397         1036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_104569_length_1036_cov_3.913354, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000397 DAOSYN010000000
VERSION     DAOSYN010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104569_length_1036_cov_3.913354"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLJ11_09085"
     CDS             complement(<1..365)
                     /locus_tag="PLJ11_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HOO04861.1"
                     /translation="MAREVAWRVFAAEFNSSSYEIKGEGEKAPSYLISPLGAKINRVF
                     VVGVLTDKENVGNGNEPVWRARITDPTGTFYLTAGQYQPEAAMALAKMEPPCFVAVVG
                     KFRTYSPEEGKLYVSIRPER"
     gene            complement(365..>1036)
                     /locus_tag="PLJ11_09090"
     CDS             complement(365..>1036)
                     /locus_tag="PLJ11_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04862.1"
                     /translation="DDTGSAELTIWKDAELQEGAEVRIVGAYVKSWRGIPKLNLGDKA
                     EVQVLAAGTLGDLAGIAPKARTLLDVELSGGGMDVVVRGTIVDVREGSGLVMRCPECR
                     RVLQKGTCRVHGKVKGVDDLRIKAILDDGTSTMNVVFGKELTEALLNMSIDEAVRLTT
                     EERNPDAVKELAEERLFARTLEVRGMVRSDDFGPMMIAREARAVEVDAREEGAKLLTE
                     LEGFE"
BASE COUNT          154 a          406 c          272 g          204 t
ORIGIN      
        1 cgctccggcc ggatggacac gtagagcttc ccctcctccg gcgagtatgt tctgaacttg
       61 cccaccaccg ccacgaagca cggcggctcc atcttcgcca gggccatggc cgcctccggc
      121 tggtactgcc ccgcggtcag gtagaacgtt ccagtgggat cggtgatcct ggcccgccag
      181 accggctcgt tgccgttgcc cacgttctcc ttgtccgtca gcaccccgac gacgaagacg
      241 cggtttatct tggccccgag gggggatatc aggtaggacg gggccttctc cccctccccc
      301 tttatctcgt agctggagga gttgaactca gcggcgaaaa ccctccaggc gacctccctg
      361 gccatcactc gaacccctcc agctcggtaa gcagcttggc gccctcctcc ctcgcgtcca
      421 cctccaccgc cctggcctcc cgggcgatca tcataggtcc gaagtcatcg ctccgcacca
      481 tgccccgcac ctccaaggtt cgggcgaaga gcctctcctc cgccagctcc ttgacggcgt
      541 ccgggttgcg ctcctcggtg gtcagcctca ccgcttcatc gatgctcatg ttcagcagcg
      601 cctcggtcag ctccttgccg aacacgacgt tcatcgtgga tgtcccatcg tccaatatcg
      661 ccttgatccg aaggtcgtcc acccccttca ccttgccatg cacccggcac gtgcccttct
      721 ggaggaccct gcggcattcg gggcagcgca tcacaagccc gctcccctcc cgcacgtcca
      781 ctatggtgcc cctcaccacg acgtccatgc ccccgccgct cagctccacg tccagtaggg
      841 tgcgcgcctt gggggcgatg cccgccaggt ccccaagggt gcccgcggca agcacctgga
      901 cctcggcctt gtccccgagg ttgagcttgg gtatgcccct ccagcttttt acataggccc
      961 ctacgatgcg cacctcggcg ccctcctgga gctcagcatc cttccagatg gtcaactccg
     1021 cgctccctgt gtcatc
//
LOCUS       DAOSYN010000398         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_104808_length_1035_cov_1.098980, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000398 DAOSYN010000000
VERSION     DAOSYN010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104808_length_1035_cov_1.098980"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1035
                     /gene="rfbH"
                     /locus_tag="PLJ11_09095"
     CDS             <1..>1035
                     /gene="rfbH"
                     /locus_tag="PLJ11_09095"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HOO04863.1"
                     /translation="LTGGRFTDRFEKGLGEYLGMKYVSLVNSGSSANLLSFMALTSPR
                     LKDRRILPGDEVITVAAGFPTTVSPIIQYGAVPVFLDVTLPEYNIDARMLDEALTDRT
                     KAVMLAHTLGNPFDLQRVKDFCDQNDLWLIEDNCDSLGSRYNYNGEWRYTGTIGDLGT
                     SSFYPPHHITMGEGGAIYTNRKDLAMIVNSMRDWGRDCHCATGQDNACGRRFMQQFGE
                     LPYGYDHKYVYSHFGYNMKATDMQAAIGCAQLEKLPAFNDARKRNWKVLRDGLADLER
                     FFIMPEPAPRSDPSWFGFMMTVRPGSDISRDSIVDHLESRQVQTRMLFAGNLIKHPCF
                     DEMRKGRKGYR"
BASE COUNT          248 a          301 c          285 g          201 t
ORIGIN      
        1 ctgaccgggg gccgtttcac cgatcggttc gagaaagggc tcggtgaata cctagggatg
       61 aaatacgttt cccttgtgaa ctccggctcc tccgcaaacc tgttgagctt catggccttg
      121 acatcgccca gattaaagga ccgaaggata ctcccgggcg atgaggtcat cacagtcgct
      181 gcagggttcc ctaccacggt atcccccata atccaatacg gggccgtccc agtgttcttg
      241 gacgtcaccc ttcccgagta caacatcgac gctcgaatgc tggacgaagc gctcacggac
      301 aggaccaagg ccgtaatgct ggcccacacc ctcggaaacc cattcgacct ccagagggtc
      361 aaggacttct gcgatcagaa cgacctctgg ctaatagagg ataactgcga ctccctgggc
      421 tctcgctaca attacaatgg cgaatggagg tatactggga cgatcggcga tctgggcacc
      481 tcctccttct atccgcccca tcacataacc atgggggagg gcggggcgat ctacaccaac
      541 agaaaggact tggccatgat cgtgaactcg atgagggact ggggccgcga ttgccactgc
      601 gccacaggac aggacaacgc ctgcggaagg cgcttcatgc agcagttcgg agagctcccc
      661 tacggctatg accacaaata tgtgtattcc cacttcggct ataatatgaa ggccacggac
      721 atgcaggccg ccatcgggtg cgcccagctc gaaaagcttc cagccttcaa cgatgcgagg
      781 aaaaggaatt ggaaggtcct gcgggacggg cttgcggacc ttgaaagatt cttcataatg
      841 ccagagccag ccccccgttc cgatccaagc tggttcgggt tcatgatgac cgtcaggccc
      901 ggttcggaca tttctcggga cagtatagtt gaccatttgg aatcaagaca ggtccagacc
      961 cgcatgcttt tcgcaggcaa cctgatcaag cacccatgct tcgacgagat gcgcaagggc
     1021 aggaaaggat acagg
//
LOCUS       DAOSYN010000399         1034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_104854_length_1034_cov_2.814096, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000399 DAOSYN010000000
VERSION     DAOSYN010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104854_length_1034_cov_2.814096"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLJ11_09100"
     CDS             complement(<1..738)
                     /locus_tag="PLJ11_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04864.1"
                     /translation="MDPPTPLTFNDKVAAFLADASTELNGQQGLRALVREEARHQWRR
                     ALPPLALMLLSAVLILQYPRALLGWFIACLFLYSWNFVFLLIPSKDGNERPKGMAGGG
                     WKQGWKAVGKLLSKPRLAVEIGLTLFLGRMVPLTIGFTLILGTGLVVLVCLVLNDYTA
                     VAGLANLIALQVGMIITFYVLVNVLRPQAQGISVIARSWKRRMGEAKGRGRLASFLVK
                     GAAVGIVVVVAVLIIGALVLPGFTFLTL"
BASE COUNT          209 a          348 c          306 g          171 t
ORIGIN      
        1 aagggtcaag aaggtgaagc ctggtagcac caacgccccg atgatgagaa ccgccacaac
       61 aaccactatg cccacagcgg cgcccttgac caagaaagag gcgagccgtc cccgtccctt
      121 cgcctccccc atccgccgtt tccaggagcg ggctatgacc gagatccctt gggcctgagg
      181 cctgaggacg ttgaccagga catagaaggt gatgatcatg cccacctgga gcgcgatcag
      241 attggccagg cctgcgaccg ccgtgtagtc attgagcacc aggcacacca atacgaccag
      301 ccctgtcccg aggatgaggg tgaacccgat ggtcaggggg accatccgcc ccaggaagag
      361 cgtgagcccg atctccacgg ccagcctggg cttgctcaac agcttcccga ccgccttcca
      421 tccctgcttc cagccgcccc ccgccattcc cttcggccgc tcgttcccat ccttcgaagg
      481 gatgagcagg aagacgaagt tccaggaata gaggaaaagg caggcgatga accagcccag
      541 gagcgcccgc gggtattgga ggatcaggac tgcgctcagg agcatcaagg ccaacggcgg
      601 cagcgccctc cgccactggt gccgcgcctc ctcccgtacc agcgcacgca gcccctgctg
      661 accgttcagc tccgtggagg catccgccag gaaggcggcg accttgtcgt tgaaggtgag
      721 gggggtggga gggtccatgc ctttcgaaca aaaaggctgg ccatctaaaa aaggattcgc
      781 atctccctcg cggcccaatg ggagggaggc ctattccttg cccggaagcc cgggtgcagc
      841 ctcgatcgga cgccccggcc ttggcagtgc actgcccatg cgtcccgttc tcctgtccct
      901 cacccgtgtc aggcgtcggg ccacaggttg gcggccatct ccctcagctt gaccttcatt
      961 atcttcccgc tggcggtcat ggggaactca tccaggaaat gcacgtacct agggaccttg
     1021 tagaaggcga tccg
//
LOCUS       DAOSYN010000400         1029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_105687_length_1029_cov_1.571869, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000400 DAOSYN010000000
VERSION     DAOSYN010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105687_length_1029_cov_1.571869"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..>1029)
                     /locus_tag="PLJ11_09105"
     CDS             complement(272..>1029)
                     /locus_tag="PLJ11_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04865.1"
                     /translation="KILEAVRTAYECGFRTMIVESHPRMKEFMKLYEKDGTFKMNFIL
                     QLPFVSGYVRHMAEGGVRGVAREVLAGTPRWELVKAPFALLSPLARKDYYAIGVKGLD
                     LEMSKYSDFDISGVLLHNIITDVLMSLDAETAFAGYFDRVQRRFGVKAGLITLNLPLL
                     SSHLNQWGLEPSVIMSPMNPYGFDMNPSQEVVEEYVRGKKVRTLAMNVLGGGSVPLSE
                     AATYIKGLGGVEGVVIGASSRKHMEELTSAFRE"
BASE COUNT          230 a          323 c          248 g          228 t
ORIGIN      
        1 ggaaacgttc tttggaaagg ctccgtcggc cgaggccaac ttttcgcccc catcatgccc
       61 aatgaacttc gagcgtcgca atgccagtat atgcaattgc acggatcgtg gcagatgtcg
      121 ggccaattcg gtcacatacg tgccggcccc accccagacc gggaagaaat caggcgctag
      181 aagaagaacg ttcatatcat ccccagtcca ccaggggcgg cattcctgta tggtggcgta
      241 tcgacctgca gcatattgaa cgccctcctc gctattctcg gaaggccgat gtcagctctt
      301 ccatatgctt tctgctagat gcccctatca ccactccttc caccccaccc aagcctttga
      361 tgtacgtggc agcctcagaa agggggaccg accctccgcc caatacgttc atggccaagg
      421 ttcgcacctt ttttccccgt acgtactcct ccaccacctc ttgggagggg ttcatatcaa
      481 atccatatgg attcataggg gacatgatca cgctaggttc cagcccccac tggttaagat
      541 ggctggatag gagcggaagg ttcagtgtga tcaaccccgc ttttacccca aaacggcgtt
      601 gaacccgatc aaagtacccg gcgaaggccg tctctgcgtc tagggacata agcacatccg
      661 ttattatatt atgcaacagc accccgctga tgtcgaagtc gctgtacttg gacatctcaa
      721 ggtcgagccc cttcactcct atggcgtagt agtccttgcg tgccaatggg gacagcaacg
      781 cgaacggggc cttgaccagc tcccatctag gggttcctgc caagacctcc ctggccacgc
      841 cgcgcacgcc tccctcagcc atatgccgga cataccctga aacgaaggga agctgaagga
      901 tgaagttcat cttgaacgtt ccatccttct catagagctt catgaactcc ttcatgcgtg
      961 gatgggattc cactatcatc gtcctgaacc cgcactcgta cgcagtgcgc accgcctcga
     1021 ggatcttcg
//
LOCUS       DAOSYN010000401         1028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_105733_length_1028_cov_3.918808, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000401 DAOSYN010000000
VERSION     DAOSYN010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105733_length_1028_cov_3.918808"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLJ11_09110"
     CDS             complement(<1..441)
                     /locus_tag="PLJ11_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOO04866.1"
                     /translation="MSDLGLVHVYTGNGKGKTTAALGMCVRAVGHGLRALVVQFMKTG
                     GTYGEGSLDLPGLRVVPSGHSCLVFSKEITQQDRDMAAEGLATAAEAMRARGYDIVVL
                     DEVNVAIKLGLLDARDVVEAVRSRAAGVEVVLTGRYAPPEILDIA"
     gene            complement(443..>1028)
                     /locus_tag="PLJ11_09115"
     CDS             complement(443..>1028)
                     /locus_tag="PLJ11_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO04867.1"
                     /translation="GGMAIKVVAFNGSPRANGNTHQALQVVLDELAREGIGTELFDIG
                     REEIRPCRACGSCGKNRDGRCVITEDRMNEHIQRIVEAQGVIIGSPVYFGNMTAQTKA
                     FADRAFYVAKGNGDMFRRKVGAAVAVNRRAGALDTFDEINKLFLISQMIVPGSSYWNT
                     VTALRPGDLAKDEEGLRTLRTLGQNMAWLLKRLE"
BASE COUNT          151 a          388 c          296 g          193 t
ORIGIN      
        1 ggcgatgtcc aatatctccg gcggggcgta cctgccggtg agcacgacct ccactcccgc
       61 agctcgggag cggaccgcct cgaccacgtc ccgtgcgtcc aggagcccga gcttgatggc
      121 cacgttgacc tcgtccagta cgacgatgtc ataccccctg gcccgcatcg cctccgccgc
      181 cgtcgcaagc ccctcggccg ccatgtccct gtcctgctgg gtgatctcct tcgagaacac
      241 caggcagctg tgccccgagg gcacgacgcg cagccctgga aggtccaggg acccttcccc
      301 gtaggtgccc cccgtcttca tgaactgcac caccaacgcg cggagcccgt gccccaccgc
      361 ccggacgcac atgcccaggg cggccgtcgt cttccccttg ccattcccgg tgtagacgtg
      421 caccagcccc aggtccgaca tctcattcca gccttttcag gagccaggcc atgttctgcc
      481 cgagggtgcg cagggtgcgt aggccctcct cgtccttggc caggtcgcct ggcctgaggg
      541 cggtgaccgt gttccaatag ctgctgccgg gcacgatcat ctggctaatg aggaagagct
      601 tgtttatctc atcgaacgtg tccaaggccc cggcgcgccg gttcaccgcc accgccgccc
      661 cgaccttgcg gcggaacatg tcgccgttgc ccttggcgac gtagaaggcg cggtccgcga
      721 acgccttcgt ctgcgcggtc atgttcccga agtacacggg ggagccgatg atgaccccct
      781 gggcctccac gatcctctgg atatgctcgt tcatccggtc ctcggtgatc acgcacctcc
      841 cgtccctgtt ctttccgcag gagccgcacg cccggcaggg cctgatctcc tcgcggccga
      901 tgtcgaacag ctccgtcccg atgccttccc tggccagctc gtcgagcacc acctgaaggg
      961 cctgatgggt gttgccattg gcccgggggc ttccattgaa cgccaccact ttgattgcca
     1021 tccctccc
//
LOCUS       DAOSYN010000402         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_106046_length_1026_cov_2.693100, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000402 DAOSYN010000000
VERSION     DAOSYN010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106046_length_1026_cov_2.693100"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..786
                     /gene="rpe"
                     /locus_tag="PLJ11_09120"
     CDS             139..786
                     /gene="rpe"
                     /locus_tag="PLJ11_09120"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOO04868.1"
                     /translation="MVKVAPSILSADFARLGEEVRRVQAANADWVHVDVMDGVFVPNL
                     TIGPEVIRALRPYATIPFDVHLMIEMPERYLERFAESGADLLTVHVEATHDVKGTLAH
                     IRRLGMRAGVSLNPGTELARVEPYLKDVDLLLIMAVQPGFGGQPFRPEVLPKIAQARE
                     ARDRDGLEFEIVIDGGINRETGRMCAERGATVLAAGSALFKNKDMASEIALWHRF"
BASE COUNT          217 a          274 c          347 g          188 t
ORIGIN      
        1 cgctcggcgg ggtggcagga caggtgagcc agaacatcaa ggtgcccttg ctcttggtgc
       61 cttagcccgg ccgatgggcg catccaaagt atttttaccg aggggggcat ctctggaccg
      121 ttcatccgag gtcgcacgat ggtgaaggtc gctccctcca tactttcagc cgacttcgcc
      181 cgattgggcg aggaggtccg ccgggtccag gcggccaatg ccgattgggt ccacgtggac
      241 gtgatggacg gcgtcttcgt gccgaacctg acgatcggcc cggaggtcat aagggccctc
      301 aggccgtacg ccaccatccc tttcgacgtc catctcatga tagagatgcc tgagaggtac
      361 ctggagcggt tcgctgaatc gggcgcggac ctgctgacgg tgcatgtgga ggccacccac
      421 gacgtcaagg ggacgctagc gcacatcagg aggctcggga tgagggcggg cgtctccttg
      481 aacccaggaa cggagctggc cagggtggag ccatatctaa aggacgtcga tttgctgttg
      541 atcatggccg tacagcctgg gttcggaggg cagcccttca ggcccgaggt gcttccaaag
      601 atagcccagg ccagggaggc cagggacagg gacgggctcg agttcgagat cgtcatcgac
      661 ggcgggatca accgggagac cggccggatg tgcgcggaaa ggggggcgac cgtcctagct
      721 gccggttccg ccctgttcaa gaacaaggac atggcctccg agattgccct ttggcacagg
      781 ttctgatacg cgggaccgat agcaagtata ataatggtga accgaaatct agggcaacct
      841 tgtcgtattt aagcgatggg gtgaggtcaa catcgggagg ggtccgatca tggcgcgaat
      901 aggttccaag aaatcattgg ttgccaagct gggcataaag gaaggtcaga gaataaggat
      961 aatcaacgcc ccttccaagt acgacctggc cttgggacgc ctgcccaaga acgtggtgga
     1021 agcggg
//
LOCUS       DAOSYN010000403         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_107343_length_1018_cov_1.950156, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000403 DAOSYN010000000
VERSION     DAOSYN010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107343_length_1018_cov_1.950156"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1018)
                     /locus_tag="PLJ11_09125"
     CDS             complement(<1..>1018)
                     /locus_tag="PLJ11_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04869.1"
                     /translation="ELVGSEDYLRLKRQMDRWCALWFWPSREEFSTQDVPLPSWFYRT
                     PADDEGALNVIAREVAREMRFFHWEIEFPDVFVRDNPGFDAVVGNPPWDRVKPESVPF
                     FTRHDPLYPTYGKQAAMRRAAEMFERNAAVKEEWREASLRTKNMAFWAKSARSPFAAS
                     LGGGRRKESDWDAHIERRIGGREAWYQSDEGGKSLRPFSLQGEGDVNLYKLFAEQGLF
                     LTREKGRMGMVLPGAVYSDYGARELRKAFLDNYGWEWLFSFENRLKIFPVDSRYIFCC
                     IVLQGGKEAEGLRTAFMVHELERWGEEGFFGVRMGKDMIKRFSPWAMVPPAVNPPDRT
                     QHGTS"
BASE COUNT          183 a          351 c          275 g          209 t
ORIGIN      
        1 gaggtgccgt gctgggtccg gtcgggagga tttaccgccg gcggcaccat ggcccaaggg
       61 ctaaacctct tgatcatgtc cttgcccatt cgtacgccga agaagccctc ctcgccccac
      121 ctctccagct cgtggaccat gaaggcggtc ctcagccctt cggcctcctt tcccccctgc
      181 aggacgatgc agcagaagat gtatctcgaa tcaacgggga atatcttaag ccggttctcg
      241 aagctgaaca gccattccca gccgtaattg tccaggaagg ccttgcgcag ctccctcgcc
      301 ccgtaatcgg aatagaccgc gcctgggagc accatcccca tccgcccctt ttcccgcgta
      361 aggaagaggc cttgctcagc gaagagcttg tagaggttga catccccctc cccttgcagg
      421 gagaacggcc tcaaggactt gcccccctca tcgctttgat accacgcctc tcggccgcct
      481 atcctgcgct cgatatgtgc atcccagtcg ctctccttcc tcctcccgcc accgaggctg
      541 gcagcgaagg gcgaacgtgc gctcttggcc caaaaggcca tgttctttgt cctgagggat
      601 gcttccctcc attcctcttt tacagccgcg ttccgttcga acatctcggc cgcgcggcgc
      661 atcgccgcct gcttcccgta ggtgggatag agggggtcgt ggcgcgtgaa aaagggcacg
      721 ctctccggtt ttaccctgtc ccaaggggga ttgcccacca cggcatcgaa gccggggtta
      781 tcgcgaacga acacgtccgg gaactcgatc tcccagtgga agaagcgcat ctcccgtgcc
      841 acctccctgg cgatgacgtt caatgcgcct tcatcgtccg ccggggtgcg atagaaccag
      901 gagggcaggg gcacatcctg tgttgagaac tcctccctgc tcggccagaa ccagagggcg
      961 caccatcggt ccatctgcct tttgagcctc agatagtcct cgctcccgac cagttcgt
//
LOCUS       DAOSYN010000404         1007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_109055_length_1007_cov_4.281513, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000404 DAOSYN010000000
VERSION     DAOSYN010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109055_length_1007_cov_4.281513"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLJ11_09130"
     CDS             complement(<1..195)
                     /locus_tag="PLJ11_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HOO04870.1"
                     /translation="MRVVLDTSGLFGLEQLPPDWEAFVTEGVLAELRRYGDRRAETLI
                     PLLKVSQATSGSLAVVREAAG"
     gene            290..553
                     /locus_tag="PLJ11_09135"
     CDS             290..553
                     /locus_tag="PLJ11_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO04871.1"
                     /translation="MPSIVVEQGVIDFILQTGQDYRICTSCSGPVMLPVALKSPKTSD
                     IVIEVGENKLYVSRVQARYLRAITSDMIYDPEVGLSCQAYPGL"
     gene            complement(538..>1007)
                     /locus_tag="PLJ11_09140"
     CDS             complement(538..>1007)
                     /locus_tag="PLJ11_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOO04872.1"
                     /translation="QKMPLLIAPHGENQALIKEGCRSAYQCVLCKRLMLLTAQGVAKG
                     IGAEGMVMGDSLGQVASQTLGNIRAEQHGLELPVLRPLIGLDKLEIEHLAKTIGTYDI
                     SIMSDGGKDCTAVPLRVVTNASYRDMMAEEGKLDLMGIAARSAERALKDYRPG"
BASE COUNT          178 a          338 c          286 g          205 t
ORIGIN      
        1 ccctgcggcc tccctcacga ccgcgagcga gccggaggtg gcctgggaga ccttgagcag
       61 cgggatgagg gtctccgccc tccggtcgcc gtatctcctg agctccgcca gcacgccctc
      121 cgtgacgaac gcctcccaat ccggagggag ctgctccagc ccgaagaggc ctgacgtgtc
      181 caataccacc ctcatgccaa agggattgcc taaggcgcta gaaaactttg cgcagggcga
      241 gggttcctcc cgtggaggtt aaatagcagc tcctgctaaa cggggcgtga tgccatcaat
      301 agttgtggag cagggcgtga tcgatttcat ccttcagacc ggacaggact atcgcatatg
      361 cacctcgtgt tccggacctg tcatgttgcc cgtcgccctc aagtccccca agaccagcga
      421 catcgtgatc gaggtcgggg agaataagct gtacgtttcc agggtgcagg ccaggtacct
      481 gcgcgcaata acctcggaca tgatctacga ccccgaggtc gggctgtcct gccaggccta
      541 tcccggccta taatccttca atgccctttc cgcggagcgg gctgctatgc ccatcaggtc
      601 cagtttccct tcctccgcca tcatgtccct gtaggaagcg ttggtgacga cccgcaacgg
      661 gaccgcggtg cagtccttgc ccccgtcgct cattattgat atgtcatagg tcccgatcgt
      721 cttggccaaa tgctcgatct ccagcttgtc cagtccgatg agcggcctca gcacgggcag
      781 ctccaggcca tgctgttccg cccttatgtt gcccagggtc tgcgaggcca cctgccccag
      841 ggaatcgccc atgaccatgc cctcggcccc tatccccttc gccactccct gcgcggtgag
      901 aagcatgagc cgcttgcata gcacgcactg gtaggcgctc ctgcagccct ctttgatcag
      961 ggcctggttc tccccgtggg gcgcgatgag aaggggcatc ttctggc
//
LOCUS       DAOSYN010000405         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanomassiliicoccales archaeon isolate AMR_MDS_2084
            NODE_109553_length_1004_cov_10.017914, whole genome shotgun
            sequence.
ACCESSION   DAOSYN010000405 DAOSYN010000000
VERSION     DAOSYN010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864151
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanomassiliicoccales archaeon (wastewater metagenome)
  ORGANISM  Methanomassiliicoccales archaeon
            Archaea; Candidatus Thermoplasmatota; Thermoplasmata;
            Methanomassiliicoccales.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,786
            Genes (coding)                    :: 1,785
            CDSs (with protein)               :: 1,785
            Genes (RNA)                       :: 43
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Methanomassiliicoccales archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109553_length_1004_cov_10.017914"
                     /isolate="AMR_MDS_2084"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906667"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1004
                     /gene="argH"
                     /locus_tag="PLJ11_09145"
     CDS             <1..>1004
                     /gene="argH"
                     /locus_tag="PLJ11_09145"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOO04873.1"
                     /translation="ITLMDALLDRATAHRHIIVPGYTHLQQAQPISMGHYWLAHFWAL
                     ARDFRRVLFAMESVNECPLGSGALAGSTLPLDRGFTTVMLGFDRTSDNSMDSVAHRDH
                     LLDCQYAMTVLMVHASRMAEDLVIYNSREFGWVVLPDAFCTGSSMMPQKKNPDVLELV
                     RGRTGRVIGDLVDLLTIIKGLPLAYNRDMQEDKRALWSSTQIVKDVLDVLPQLIARVE
                     IDAERAAEAFSDGFTLATDVAEYLVMKGIPFRTAHEQVGEAVRWCLDRKRTLLSLTRE
                     EWNELIPYSGDDLMPILNIESSVSRRNTQGGTSFKQVALQIERGLETLEVFRKKLAAY
                     P"
BASE COUNT          204 a          312 c          311 g          177 t
ORIGIN      
        1 ataacgctga tggacgccct gctcgacagg gccacggctc acaggcacat catcgtgccg
       61 ggctatactc acctccagca ggcccagccg atcagcatgg gtcattactg gctggcccac
      121 ttctgggccc ttgccaggga cttccgcagg gtgctcttcg ccatggagtc ggtcaacgaa
      181 tgccccctcg ggagcggagc cctggcaggc tccacccttc ccctggaccg tgggttcacc
      241 acggtcatgc tgggtttcga caggaccagc gacaacagca tggactctgt ggcgcacagg
      301 gaccacctcc tggactgcca gtacgccatg acggtcctga tggtccacgc gagcaggatg
      361 gccgaggacc tggtgatcta caacagccgc gaattcgggt gggtcgtcct tcccgacgca
      421 ttctgcaccg gctccagcat gatgccccag aagaagaacc ccgatgtcct cgagttggtg
      481 agggggcgga cgggacgggt catcggcgac ctggtggatc tgcttacaat catcaagggc
      541 cttcccctgg cctacaacag ggacatgcag gaggacaagc gggccctgtg gtcgtcgacc
      601 cagatagtca aggatgtcct cgacgttctc ccccagctca tcgcccgcgt tgagatcgac
      661 gcagagaggg ccgcggaggc cttttcggac ggcttcaccc tggcgacgga cgtggccgag
      721 tacctggtta tgaaggggat tcccttcagg accgcccatg aacaggtggg cgaagcggtc
      781 cggtggtgcc tcgacaggaa gaggaccctc ctgagcctga ccagggagga gtggaatgaa
      841 ctcatcccct atagcggcga tgacctgatg ccgatcctga acatcgagag ttccgtatcc
      901 aggaggaaca cccagggagg gacttccttc aagcaggtag ccctacagat agagcggggg
      961 cttgagacgc tcgaggtatt ccgtaaaaaa ctcgccgcct accc
//
